An analysis of class I antigens of man and other species by one-dimensional IEF and immunoblotting.
A procedure for the molecular identification of MHC class I products based on 1-D IEF and subsequent immunoblotting is described. Optimal conditions for 1-D IEF, the electrophoretic transfer of proteins out of denaturing, nonionic detergent-containing gels to nitrocellulose, and the requisite antibodies, both polyclonal and monoclonal, for the visualization of class I heavy chains have been established. Cross-reactivity of antibodies has enabled the biochemical analysis of class I heavy chains in the dog. The procedure reported here requires modest amounts of cells and allows a rapid molecular characterization of class I heavy chain polymorphisms in man and other species without the need for radiochemical methods.
D000818:Animals; D002415:Cats / Q000276:immunology; D004285:Dogs / Q000276:immunology; D006680:HLA Antigens / Q000032:analysis*; D006649:Histocompatibility Antigens / Q000032:analysis*; D006801:Humans; D007122:Immunoelectrophoresis; D007525:Isoelectric Focusing; D008285:Major Histocompatibility Complex; D051379:Mice / Q000276:immunology; D013045:Species Specificity
D006680:HLA Antigens; D006649:Histocompatibility Antigens