Adoptive transfer of autoimmune diabetes mellitus in biobreeding/Worcester (BB/W) inbred and hybrid rats.
Adoptive transfer of diabetes was accomplished by the injection of Con A-activated acutely diabetic BB/W rat spleen cells into immunosuppressed diabetes-resistant BB/W control rats and F1 hybrid offspring produced by BB/W X Lewis, BN, Yashida, and NEDH matings. Immune suppression methods that facilitated adoptive transfer of diabetes included neonatal thymectomy, cyclophosphamide, and splenectomy plus rabbit anti-rat lymphocyte serum injections. The successful transfer of BB/W diabetes to otherwise normal (BB/W X inbred)F1 rats and to diabetes-resistant BB/W animals suggests that antigenically normal pancreatic beta cells were destroyed by the injected effector cells. Diabetes-resistant BB/W control rats also evidenced diabetes after the injection of cyclophosphamide alone. The requirement for immunosuppression suggests that an intact immune system protects against adoptive transfer and diabetes in diabetes-resistant BB/W rats.
D000818:Animals; D000961:Antilymphocyte Serum / Q000008:administration & dosage; D001327:Autoimmune Diseases / Q000209:etiology / Q000276:immunology*; D003433:Crosses, Genetic; D003520:Cyclophosphamide / Q000008:administration & dosage; D003921:Diabetes Mellitus, Experimental / Q000209:etiology / Q000276:immunology*; D005260:Female; D007116:Immunization, Passive; D008297:Male; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains / Q000235:genetics / Q000276:immunology*; D013154:Spleen / Q000637:transplantation
D000961:Antilymphocyte Serum; D003520:Cyclophosphamide