title: Ultrastructural demonstration of anionic sites in basement membrane zone by cationic probes.
abstract: Anionic sites were demonstrated ultrastructurally in both the dermal and epidermal edges of the lamina densa using strongly cationized polyethyleneimine as a tracer. Enzyme digestion with heparitinase and pronase removed the anionic binding sites, indicating that the sites consisted largely of heparan sulfate proteoglycan.
mesh_terms: D000838:Anions; D001485:Basement Membrane / Q000648:ultrastructure*; D001665:Binding Sites; D006497:Heparan Sulfate; D006801:Humans; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D011094:Polyethyleneimine; D011509:Proteoglycans; D012867:Skin / Q000648:ultrastructure*
chemical_list: D000838:Anions; D006497:Heparan Sulfate; D011094:Polyethyleneimine; D011509:Proteoglycans