Humoral immune responses within the human central nervous system following systemic immunization.
We investigated sequential humoral immune responses in the CSF and blood of 6 stable multiple sclerosis (MS) patients without decreases in the blood-brain barrier. Anti-tetanus toxoid antibodies (anti-TT Ab) increased to a similar relative degree within the CSF and blood starting within 2 weeks after subcutaneous booster injection of TT. In 3 of 4 subjects, CSF lymphocytes obtained at 2 weeks secreted anti-TT Ab to the same degree as autologous blood lymphocytes when cultured with pokeweed mitogen. These findings suggest a prompt antibody response within the CSF to systemically administered antigen, not due to diffusion from the serum, with active trafficking of TT-sensitized lymphocytes into the central nervous system.
D000328:Adult; D000906:Antibodies / Q000276:immunology; D000917:Antibody Formation; D002478:Cells, Cultured; D002490:Central Nervous System / Q000276:immunology*; D002555:Cerebrospinal Fluid / Q000166:cytology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G / Q000032:analysis / Q000134:cerebrospinal fluid; D008214:Lymphocytes / Q000276:immunology; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology / Q000494:pharmacology
D000906:Antibodies; D007074:Immunoglobulin G; D013745:Tetanus Toxoid