Long time treatment of N1E-115 neuroblastoma cells with atropine induces changes in markers of muscarinic cholinergic function.
N1E-115 neuroblastoma cells were grown in the absence or presence of atropine (1 microM) for 9 days. After 9 days membranes were prepared from control and atropine-treated cells. They were stored frozen until some markers of muscarinic cholinergic function were measured. Atropine treatment increased the number of muscarinic receptors from 100 +/- 10 fmol/mg protein to 145 +/- 20 fmol/mg protein, decreased the cholinesterase activity from 3.5 +/- 2.0 U/mg protein to 1.0 +/- 0.5 U/mg protein and increased the choline acetyltransferase activity from 0.25 +/- 0.13 pmol [3H]acetylcholine synthesized/min X mg protein to 1.80 +/- 0.59 pmol [3H]acetylcholine synthesized/min X mg protein. It is suggested that all these changes are correlates of muscarinic receptor supersensitivity.
D000818:Animals; D001285:Atropine / Q000494:pharmacology*; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D002802:Cholinesterases / Q000378:metabolism; D002999:Clone Cells; D051379:Mice; D009447:Neuroblastoma; D009474:Neurons / Q000032:analysis / Q000187:drug effects* / Q000201:enzymology; D011976:Receptors, Muscarinic / Q000032:analysis / Q000187:drug effects*
D011976:Receptors, Muscarinic; D001285:Atropine; D002795:Choline O-Acetyltransferase; D002802:Cholinesterases