Phenotypic analysis of temperature-sensitive yeast actin mutants.
The consequences of two different mutations in the single essential actin structural gene of yeast (Saccharomyces cerevisiae) were studied. Both conditional-lethal actin mutants exhibit six phenotypes at the restrictive temperature: disruption of the asymmetric staining pattern of actin assembly; delocalized deposition of chitin on the cell surface; partial inhibition of secretion of the periplasmic protein, invertase; an intracellular accumulation of secretory vesicles; death of cells in the budded portion of the cell cycle upon prolonged incubation at the restrictive condition; and osmotic sensitivity. These results implicate actin in the organization and polarized growth of the yeast cell surface.
D000199:Actins / Q000235:genetics*; D002453:Cell Cycle; D002470:Cell Survival; D002686:Chitin / Q000032:analysis; D002875:Chromosomes; D006026:Glycoside Hydrolases / Q000378:metabolism; D009154:Mutation; D009997:Osmotic Pressure; D010641:Phenotype; D013696:Temperature; D015003:Yeasts / Q000235:genetics* / Q000378:metabolism; D043324:beta-Fructofuranosidase
D000199:Actins; D002686:Chitin; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase