IgG purification to measure the level of an iodinated thyroglobulin peptide, the 3,5,3',5' tetraiodo-l-tyrosyl-l-tyrosine in human serum.
Antibodies reacting with 3,5,3',5' tetraiodo-l-tyrosyl-l-tyrosine (I2Tyr-I2Tyr) were elicited in rabbits by immunization with an oxidized yeast conjugate coupled with I2Tyr-I2Tyr. Ion-exchange chromatography was used to purify immunoglobulins, in order to improve the specificity in measurement of I2Tyr-I2Tyr level in patient serum. IgG binding capacity versus I2Tyr-I2Tyr was considerably increased after immunoglobulin purification.
D000818:Animals; D000918:Antibody Specificity; D002852:Chromatography, Ion Exchange; D004105:Diiodotyrosine / Q000031:analogs & derivatives* / Q000097:blood / Q000276:immunology; D004151:Dipeptides / Q000097:blood* / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology / Q000302:isolation & purification*; D011817:Rabbits / Q000276:immunology
D004151:Dipeptides; D007106:Immune Sera; D007074:Immunoglobulin G; C007441:3,5-diiodo-tyrosyl-3,5-diiodo-tyrosine; D004105:Diiodotyrosine