Chemical modification of the brown fat mitochondrial uncoupling protein with tetranitromethane.
Tetranitromethane reacts with the uncoupling protein of intact brown fat mitochondria. The chloride permeability in the absence of the inhibitory nucleotide GDP is not affected, but the affinity with which GDP binds is decreased, and the coupling between binding of nucleotide and inhibition of chloride permeation is broken.
D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002352:Carrier Proteins; D002463:Cell Membrane Permeability / Q000187:drug effects; D002712:Chlorides / Q000378:metabolism; D006224:Cricetinae; D006153:Guanosine Diphosphate / Q000378:metabolism / Q000494:pharmacology; D066298:In Vitro Techniques; D007473:Ion Channels; D008565:Membrane Proteins / Q000378:metabolism*; D008647:Mesocricetus; D008697:Methane / Q000031:analogs & derivatives*; D024101:Mitochondrial Proteins; D008933:Mitochondrial Swelling / Q000187:drug effects; D013774:Tetranitromethane / Q000494:pharmacology*; D000071256:Uncoupling Protein 1; D014634:Valinomycin / Q000494:pharmacology
D002352:Carrier Proteins; D002712:Chlorides; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D000071256:Uncoupling Protein 1; D006153:Guanosine Diphosphate; D014634:Valinomycin; D013774:Tetranitromethane; D008697:Methane