Lymphonodal response to sensitization with 2,4-dinitrochlorobenzene (DNCB) in laboratory rats.
The authors investigated the lymphonodal response to peripherally administered 2,4-dinitrochlorobenzene (DNCB). DNCB is known as a strong irritant binding to membrane proteins of the Langerhans cells of the skin. The conjugate is a potent neoantigen able to induce local tumor-destruction and raise cellular immune defence. White laboratory rats were used for the experiments. Their popliteal lymph nodes were investigated after sensitization with DNCB and i.c. injection of H3-thymidine and Patent Blue Violet at several intervals following the challenge with the hapten DNCB. Changes of weight of lymph nodes further uptake of H3-thymidine by the cells were investigated by autoradiography, while the content of H3-DNA indicating an increased DNA-metabolism due to blastic transformation of the small lymphocytes, was determined quantitatively by a liquid scintillation method. The data obtained speak for the usefulness of DNCB as an immunostimulant, comparable to BCG but of less toxicity.
D000818:Animals; D001345:Autoradiography; D004247:DNA / Q000378:metabolism; D004137:Dinitrochlorobenzene / Q000494:pharmacology*; D005260:Female; D007114:Immunization; D008198:Lymph Nodes / Q000187:drug effects / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D009578:Nitrobenzenes / Q000494:pharmacology*; D009929:Organ Size; D051381:Rats; D012588:Scintillation Counting; D013936:Thymidine / Q000378:metabolism
D004137:Dinitrochlorobenzene; D009578:Nitrobenzenes; D004247:DNA; D013936:Thymidine