Inhibitory effect of 3-deazaadenosine on the thromboplastin response of stimulated human monocytes.
Immune complexes (IC), 12-O-tetradecanoylphorbol-13-acetate (TPA), endotoxin (LPS) and phytohaemagglutinin (PHA) induce thromboplastin activity in human peripheral blood monocytes. In the presence of transmethylation inhibitors 3-deazaadenosine (DZA) and homocysteine a dose-dependent inhibition of the thromboplastin response reaching about 60 per cent was observed, when IC, LPS or PHA was used as the stimulant. TPA-induced thromboplastin synthesis was more resistant (maximum 20 per cent inhibition).
D004305:Dose-Response Relationship, Drug; D006710:Homocysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008745:Methylation; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D010835:Phytohemagglutinins / Q000494:pharmacology; D012263:Ribonucleosides / Q000494:pharmacology*; D013925:Thromboplastin / Q000096:biosynthesis*; D014372:Tubercidin / Q000494:pharmacology*
D010835:Phytohemagglutinins; D012263:Ribonucleosides; C018258:3-deazaadenosine; D006710:Homocysteine; D013925:Thromboplastin; C007957:homocysteine thiolactone; D014372:Tubercidin