A semi-automated system for quantitative densitometry of autoradiographs.
We describe a semi-automatic, computerized method of quantitative densitometry for regional analysis of whole brain autoradiographs. The technique features menu-driven software and verbal prompting for data via a speech synthesizer. Advantages over manual methods include an 80% reduction in the time required for data collection, easy retrieval and statistical analysis of data, and the production of a large data base for future use. The application of this system to the [14C]2-deoxy-D-glucose autoradiographic method for measurement of local cerebral glucose utilization is described. This system makes available computerized densitometry at a modest cost.
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000378:metabolism*; D003201:Computers; D003720:Densitometry / Q000379:methods*; D003847:Deoxyglucose / Q000378:metabolism
D003847:Deoxyglucose