Characterization of an insulin-related factor secreted by a teratoma cell line.
The teratoma-derived insulin-independent cell line 1246-3A produces and secretes polypeptide mitogens in its culture serum-free medium. Mitogenic activities were separated by Sephadex G-50 chromatography into two fractions eluted, one in the void volume region, another one with an apparent Mr of 6 kDa. Only the 6 kDa mitogen presents properties similar to pancreatic insulin as estimated by radioimmunoassay, radio-receptor assay, and biochemical characterization. As a consequence, this factor is called insulin-related factor (IRF). Evidence presented in this paper indicate that ectopic IRF binds to insulin receptors on the producer cells, 1246-3A and acts in an autostimulatory manner.
D000818:Animals; D001667:Binding, Competitive; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002850:Chromatography, Gel; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D011863:Radioimmunoassay; D011869:Radioligand Assay; D011972:Receptor, Insulin / Q000378:metabolism; D013002:Somatomedins / Q000378:metabolism*; D013724:Teratoma / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism
D013002:Somatomedins; D011972:Receptor, Insulin; D013936:Thymidine