Toxicity determined in vitro by morphological alterations and neutral red absorption.
A method is described which combines the use of a visual morphological cytotoxicity assay with a quantitative neutral red (NR) spectrophotometric test, for the assessment of the effect of toxic agents on 3T3 cells in culture. These sensitive and reproducible assays lend themselves to a screening procedure of potential toxicants which can help reduce the use of animals for toxicity testing.
D000818:Animals; D002460:Cell Line; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009499:Neutral Red; D010619:Phenazines; D014116:Toxicology / Q000379:methods*
D010619:Phenazines; D009499:Neutral Red