On the coordination properties of Eu3+ bound to tRNA.
The luminescence properties of Eu3+ have been used to investigate the binding and coordination properties of the ion with tRNA, as an attempt to resolve the discussion of whether metal ions bind to tRNA in solution only by Debye-Hückel screening, or whether direct coordination to specific sites may occur. Binding studies with Escherichia coli tRNAmet/f (taking advantage of 4-thiouracil-sensitized Eu3+ emission) distinguish three classes of binding affinities. Two of these are single sites with affinities approx. 10(4) and approx. 10(3) tighter than the nonspecific affinity of Eu3+ for native DNA. Mg2+ competes for binding at both these sites. Measurement of the lifetime and excitation spectrum of Eu3+ bound to the highest affinity site shows that the ion has two to five non-phosphate ligands in its inner coordination sphere. The existence of this coordinated site demonstrates that electrostatic screening is not the only mechanism for metal ion interaction with tRNA. The coordination properties of the high-affinity Eu3+ site do not agree with the properties of any of the metal ion sites found in the two tRNAphe crystal forms. Possible reasons for this discrepancy are discussed; it may be that ions bind differently to isolated molecules in solution than to molecules packed in a crystal lattice.
D001665:Binding Sites; D001667:Binding, Competitive; D003461:Crystallography; D004247:DNA; D005063:Europium / Q000378:metabolism; D007700:Kinetics; D008163:Luminescent Measurements; D008274:Magnesium / Q000378:metabolism; D009690:Nucleic Acid Conformation; D012343:RNA, Transfer
D005063:Europium; D004247:DNA; D012343:RNA, Transfer; D008274:Magnesium