Partial characteristics of an analog of pyridinoline isolated from human skin.
The most abundant amine in acid hydrolysates of human skin, eluting in the crosslink region of a reversed-phase HPLC chromatogram, has the same retention time as pyridinoline standard. This amine is not pyridinoline, since it is a weak fluorophore and its U/V spectrum does not agree with that of pyridinoline. The unknown amine was isolated and characterized by fast atom bombardment mass spectrometry and its structure is consistent with a deoxy-analogue of pyridinoline. It may be a crosslink component of some biological importance, since it is not detectable in skin from a patient with Marfan's Syndrome.
D000588:Amines / Q000302:isolation & purification*; D000596:Amino Acids / Q000302:isolation & purification; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008382:Marfan Syndrome / Q000378:metabolism; D013058:Mass Spectrometry; D012867:Skin / Q000032:analysis*
D000588:Amines; D000596:Amino Acids; C015484:pyridinoline