An improved method for in vitro perfusion of human skin.
We have developed a procedure for establishing a long-living supravital skin preparation. Our model has made in vitro perfusion of human cutis, fatty tissue and lymph nodes possible. We describe the method of preparation, the technical construction and preliminary results of our experiments.
D046508:Culture Techniques / Q000379:methods*; D006119:Groin; D006801:Humans; D010477:Perfusion / Q000295:instrumentation / Q000379:methods*; D012867:Skin / Q000378:metabolism*; D013048:Specimen Handling / Q000379:methods; D013524:Surgical Flaps