title: Insulin binding to human ovaries.
abstract: Human ovarian tissue samples were obtained at the time of laparotomy, and plasma membrane fractions were prepared and used in receptor assays. Incubations of the membrane fraction were performed with [125I]insulin (porcine), and Scatchard analysis of binding showed biphasic curves. The high affinity sites had an average concentration of 57.4 +/- 7.9 (+/- SEM) fmol/mg protein and a dissociation constant of 3.5 +/- 0.9 nM (n = 9). Neither affinity nor number of binding sites changed significantly during the menstrual cycle. We conclude that there is high affinity binding of [125I]insulin to human ovarian plasma membranes.
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D005498:Follicular Phase; D006801:Humans; D066298:In Vitro Techniques; D008183:Luteal Phase; D010053:Ovary / Q000378:metabolism*; D011972:Receptor, Insulin / Q000378:metabolism*; D013696:Temperature
chemical_list: D011972:Receptor, Insulin