title: Effects of antimycin, glucose deprivation, and serum on cultures of neurons, astrocytes, and neuroblastoma cells.
abstract: The resistance of cultured mouse neuroblastoma cells, primary cultures of rat cerebellar neurons, and rat brain astrocytes to a block of aerobic metabolism was studied. Parameters such as lactate production and ATP content were measured in the presence of antimycin A and under various conditions of glucose, oxygen, and serum supply. The following conclusions can be drawn: (1) All cell types studied were characterized by an active production of lactate; (2) Incubation of the various cell types in the absence of glucose at normal oxygen tension did not affect ATP levels; (3) Respiration blocked by antimycin led to a Pasteur effect; (4) Neuroblastoma cells, but not the other cell types, were fully resistant to inhibition of respiration provided that sufficient glucose was supplied; (5) In the absence of glucose no stores of energy or utilizable substrate were present in the cell types studied when respiration was blocked; (6) In the presence of fetal calf serum anoxic neurons showed irreversible signs of degeneration.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000968:Antimycin A / Q000031:analogs & derivatives* / Q000494:pharmacology; D001253:Astrocytes / Q000166:cytology*; D001769:Blood; D002460:Cell Line; D002478:Cells, Cultured; D005947:Glucose; D006019:Glycolysis; D007773:Lactates / Q000096:biosynthesis; D019344:Lactic Acid; D051379:Mice; D009447:Neuroblastoma / Q000473:pathology*; D009474:Neurons / Q000166:cytology*; D010100:Oxygen / Q000378:metabolism
chemical_list: D007773:Lactates; C032456:antimycin; D019344:Lactic Acid; D000968:Antimycin A; D000255:Adenosine Triphosphate; D005947:Glucose; D010100:Oxygen