An L-lactate sensor with immobilized enzyme for use in in vivo studies with an endocrine artificial pancreas.
We report the features of a sensor for determining L-lactate. An oxygen sensor, coupled to a nylon net with chemically bound L-lactate oxidase (EC 1.1.3.2), is inserted into an artificial pancreas (Biostator, Miles) "downstream" from the glucose sensor. We used the sensor to continuously monitor the L-lactate concentration in blood after a "glucose clamp" experiment with a diabetic patient. L-Lactate determinations in blood drawn from the patient every 15 min agreed well with results obtained by use of the L-lactate sensor.
D004800:Enzymes, Immobilized; D006801:Humans; D007332:Insulin Infusion Systems; D007773:Lactates / Q000097:blood*; D019344:Lactic Acid; D006899:Mixed Function Oxygenases; D010100:Oxygen / Q000032:analysis
D004800:Enzymes, Immobilized; D007773:Lactates; D019344:Lactic Acid; D006899:Mixed Function Oxygenases; C021497:lactate 2-monooxygenase; D010100:Oxygen