Loss of antibody production accompanied by chromosome loss in a cloned hybrid line secreting antibodies to sheep red blood cells.
Somatic cell hybrids between Sp2/O-Ag14 mouse myeloma cells and lymphocytes derived from BALB/c mice hyperimmunized with sheep red blood cells (SRBC) were produced. One hybrid producing IgG1 antibody to SRBC was selected, cloned twice and subsequently transferred to BALB/c mice. After a number of transfers it was found that the antibody titer in ascites fluid gradually decreased. Cytogenetic analysis revealed gradual chromosome loss in the hybrid clone, which produced progressively less antibody.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002875:Chromosomes / Q000648:ultrastructure*; D004912:Erythrocytes / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology* / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D012756:Sheep / Q000276:immunology; D013997:Time Factors
D000911:Antibodies, Monoclonal