title: An improved electrofusion technique for production of mouse hybridoma cells.
abstract: An experimental procedure is described for the reproducible production of hybridoma cells using the electrofusion technique. High yields can be obtained when fusion is performed in isotonic inositol solutions containing Ca2+ and Mg2+ in a ratio of 1:5 in the millimolar range. The hybridoma cells are transferred 10 min after the field pulse application into a balanced salt solution for 30 min at 37 degrees C.
mesh_terms: D000818:Animals; D002459:Cell Fusion; D002999:Clone Cells / Q000166:cytology; D004560:Electricity; D006825:Hybridomas / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012996:Solutions
chemical_list: D012996:Solutions