title: Proline isomerization during refolding of ribonuclease A is accelerated by the presence of folding intermediates.
abstract: The trans----cis isomerization of Pro 93 was measured during refolding of bovine ribonuclease A. This isomerization is slow (tau = 500 s) under marginally stable folding conditions of 2.0 M GdmCl, pH 6, at 10 degrees C. However, it is strongly accelerated (tau = 100 s) in samples which, prior to isomerization, had been converted to a folding intermediate by a 15 s refolding pulse under strongly native conditions (0.8 M ammonium sulfate, 0 degree C). The results demonstrate that extensive folding is possible before Pro 93 isomerizes to its native cis state and that the presence of structural folding intermediates leads to a marked increase in the rate of subsequent proline isomerization.
mesh_terms: D007536:Isomerism; D007700:Kinetics; D008958:Models, Molecular; D011392:Proline; D011487:Protein Conformation; D012259:Ribonuclease, Pancreatic
chemical_list: D011392:Proline; D012259:Ribonuclease, Pancreatic