Blockage of tropoelastin secretion by monensin represses tropoelastin synthesis at a pretranslational level in rat smooth muscle cells.
The blockage of protein secretion in the R22 cultured rat aortic smooth muscle cell strain with monensin repressed tropoelastin gene expression at the mRNA level by ca. 50-fold as measured by biosynthetic pulse-labeling, in vitro translation, and hybridization with a tropoelastin genomic DNA probe. These results suggest that tropoelastin gene expression is autoregulated, and they represent the first reported effect of monensin on gene expression.
D000818:Animals; D004549:Elastin / Q000031:analogs & derivatives*; D005663:Furans / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D008985:Monensin / Q000494:pharmacology*; D009130:Muscle, Smooth / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012636:Secretory Rate / Q000187:drug effects; D014333:Tropoelastin / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
D005663:Furans; D012333:RNA, Messenger; D014333:Tropoelastin; D004549:Elastin; D008985:Monensin