[Mouse oocyte maturation in vitro at a low concentration of pyruvate in the culture medium].
Mouse oocytes isolated from antrum--containing follicles were cultivated for 17-19 hours in the media with different concentrations of pyruvate. Decrease of pyruvate concentration down to 0.03 mM significantly increased the number of oocytes with intact germinal vesicles. However, in 50% of oocytes desintegration of nuclear membrane and condensation of chromosomes occurred even at a concentration of 0.02 mM.
D000818:Animals; D003470:Culture Media / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development*; D011773:Pyruvates / Q000378:metabolism*; D019289:Pyruvic Acid
D003470:Culture Media; D011773:Pyruvates; D019289:Pyruvic Acid
Sozrevanie ootsitov mysheĭ in vitro pri nizkoĭ kontsentratsii piruvata v srede kul'tivirovaniia.