Monitoring lens transparency during in vitro incubation by Scheimpflug photography and densitometric image analysis.
Dragomirescu et al. [1983] described a 2-chamber flow system for the incubation of lenses with an inserted photometer to measure changes in lens transparency. Mester et al. [1984] applied the method to simulate conditions occurring during vitrectomy, which occasionally leads to reversible lens opacification. Using Scheimpflug photography (a modified SL 45 Topcon camera) instead of the transmission measurements of incubated lenses has the advantage that disorders in lens transparency can be exactly localized and the sensitivity is much higher than the photometer readings. Application of Scheimpflug photography required a modification of the incubation chamber. The application of the experimental set-up was demonstrated with pig lenses under various incubation conditions. The results obtained by densitometric image analysis of Scheimpflug photos were compared with transmission measurements by photometer.
D000818:Animals; D002386:Cataract / Q000139:chemically induced / Q000473:pathology; D003720:Densitometry / Q000379:methods; D066298:In Vitro Techniques; D007185:Incubators; D007908:Lens, Crystalline / Q000502:physiology*; D008991:Monitoring, Physiologic / Q000379:methods*; D010781:Photography / Q000379:methods; D010865:Pilot Projects; D013552:Swine; D013696:Temperature