Pyruvate prevents the ATP depletion caused by formaldehyde or calcium-chelator esters in the human red cell.
Formaldehyde released during hydrolysis of calcium-chelator esters incorporated into cells blocks glycolysis in the human erythrocyte (Tiffert, T., García-Sancho, J. and Lew, V.L. (1984) Biochim. Biophys. Acta 773, 143-156). This blockade is due to the inhibition of glyceraldehyde-3-phosphate dehydrogenase by NAD+ depletion caused by enzymatic oxidation of formaldehyde coupled to NADH production. The addition of pyruvate to the incubation medium prevents or reverts ATP depletion.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000634:Aminoquinolines / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D005557:Formaldehyde / Q000494:pharmacology*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D009243:NAD / Q000378:metabolism; D011773:Pyruvates / Q000494:pharmacology*; D019289:Pyruvic Acid
D000634:Aminoquinolines; D011773:Pyruvates; D009243:NAD; D005557:Formaldehyde; C035738:Quin2-acetoxymethyl ester; D019289:Pyruvic Acid; D000255:Adenosine Triphosphate; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases