title: Ecdysteroids accelerate mitoses in accessory glands of beetle pupae.
abstract: During the 9-day pupal period of Tenebrio molitor (the mealworm beetle), the cells of the male accessory glands undergo divisions for 7 days. There are two maxima in the mitotic activity in the glands in vivo, one at 1 day and the other at 4 days. The latter peak coincides with the large surge of ecdysterone occurring in the pupal stage. By the use of in vitro culture techniques, it has been demonstrated that the first bout of mitosis in both glands proceeds in basal medium, while the second bout of mitosis requires a physiological level of ecdysterone. Ecdysone was less effective than ecdysterone. Sensitivity to ecdysterone did not change significantly between Day 1 and Day 4 of the pupal stage. The results are discussed in relation to the effects of ecdysterone on cell division in mesodermal and ectodermal derivatives.
mesh_terms: D000367:Age Factors; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D004440:Ecdysone / Q000494:pharmacology; D004441:Ecdysterone / Q000494:pharmacology*; D008297:Male; D008855:Microscopy, Electron, Scanning; D008938:Mitosis / Q000187:drug effects*; D009924:Organ Culture Techniques; D011679:Pupa / Q000033:anatomy & histology / Q000166:cytology; D013712:Tenebrio / Q000166:cytology / Q000254:growth & development*
chemical_list: D004440:Ecdysone; D004441:Ecdysterone