Characterization of the Fc(IgG) receptor on the pluripotential leukaemia cell K-562.
K-562 cells express an Fc receptor that is murine isotype IgG specific. The receptor was defined by rosette formation using sheep erythrocytes sensitized with murine monoclonal haemagglutinin (EA) of known isotype. Optimal rosette formation occurred at 4 degrees C or ambient temperature; however, the number of rosettes formed at ambient temperature decreased after 8 h whereas formed rosettes were stable at 4 degrees C. EA in which IgG2b was the immunoglobulin isotype gave high numbers of rosettes while IgG2a gave lower but significant numbers. Aggregated human IgG inhibited rosette formation of EA(IgG2a) more easily than those of EA(IgG2b), indicating a higher affinity of the Fc receptor for IgG2b.
D000818:Animals; D002460:Cell Line; D007074:Immunoglobulin G / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology; D007938:Leukemia / Q000276:immunology*; D051379:Mice; D011961:Receptors, Fc / Q000276:immunology*; D012397:Rosette Formation
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011961:Receptors, Fc