[Cause of dehydroascorbic acid accumulation in the blood of patients with insulin-dependent diabetes mellitus].
It is known that dehydroascorbic acid (DHAA) produces a diabetogenic effect and its content in the blood increases in diabetes mellitus. It was previously established that the generation of reducing equivalents (RE) in the course of hexosemonophosphate shunt, CO2 production and SH-glutathione regeneration in erythrocytes with and without moderate and maximum oxidation load in vitro were not disturbed in diabetes. The authors have proposed a procedure to study blood and erythrocyte DHAA reductase activity in suspension in health and in insulin-dependent diabetes mellitus by means of redoxstatometry using a device of original design. A significant acceleration of RE transfer through the erythrocyte membrane was detected in diabetes. A lowered participation in this process of the AA in equilibrium DHAA "shuttle" system was recorded in the blood of patients with diabetes mellitus what was mostly expressed under the conditions of acidosis in vitro. Probably "shuttle" function in diabetes was provided by some other redox system which might be located in the plasma. The predominant functioning of this redox system and a decrease of DHAA reductase activity in diabetes resulted in the accumulation of DHAA in the blood of patients with type I diabetes mellitus.
D000328:Adult; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D001692:Biological Transport; D003470:Culture Media; D003683:Dehydroascorbic Acid / Q000097:blood*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D004910:Erythrocyte Membrane / Q000378:metabolism*; D005980:Glutathione Reductase / Q000097:blood; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008657:Metabolic Clearance Rate; D008875:Middle Aged; D010084:Oxidation-Reduction
D003470:Culture Media; D005980:Glutathione Reductase; D001205:Ascorbic Acid; D003683:Dehydroascorbic Acid
O prichine nakopleniia degidroaskorbinovoĭ kisloty v krovi bol'nykh insulinzavisimym sakharnym diabetom.