[Isolation of neutral proteinase of ribosomes (cathepsin R)].
A method for isolation of cathepsin R from rat liver ribosomes allowing for a 264-fold increase of specific activity is described. The purification procedure includes enzyme extraction from ribosomes with 2-4 M LiCl and two-step affinity chromatography on Sepharose with immobilized soy bean trypsin inhibitor and trypsin-Sepharose.
D000818:Animals; D002403:Cathepsins / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000201:enzymology; D008297:Male; D051381:Rats; D012270:Ribosomes / Q000201:enzymology*; D012697:Serine Endopeptidases
C035145:ribosomal neutral proteinase; D002403:Cathepsins; D012697:Serine Endopeptidases
Vydelenie neĭtral'noĭ proteinazy ribosom (katepsina R).