Dolichol-sugar derivative synthesis in human breast cancer cell line (T47D). Effects of estrogens and antiestrogens.
In the present paper we report evidence about the formation of polyprenyl-phosphate monosaccharides, their elongation products and the assembly of dolichyl-diphosphate-oligosaccharide to endogenous T47D clone 11 proteins upon incubation with [14C]glucose. The influence of estradiol and two nonesteroidal antiestrogens -nafoxidine and tamoxifen- was examined on the dolichol pathway in T47D cell cultures. Estradiol (1 nM) does not change the rate of synthesis of dolichyl-phosphate-sugar derivatives in contrast to nafoxidine and tamoxifen both a micromolar concentration, which induce a remarkable decrease in the formation of dolichol-sugar derivatives. In addition, T47D cells were pretreated with nafoxidine or tamoxifen during one hour, fresh medium supplemented with estradiol was added to the cells simultaneously with [14C]glucose. Results indicated that estradiol after nafoxidine induces a slight increase in the polyprenyl-sugar derivatives formation, however, estradiol after tamoxifen decreases the synthesis of these substances.
D001943:Breast Neoplasms / Q000378:metabolism*; D002460:Cell Line; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D009256:Nafoxidine / Q000494:pharmacology*; D011103:Polyisoprenyl Phosphate Monosaccharides / Q000096:biosynthesis*; D011105:Polyisoprenyl Phosphate Sugars / Q000096:biosynthesis*; D011759:Pyrrolidines / Q000494:pharmacology*; D013629:Tamoxifen / Q000494:pharmacology*
D011103:Polyisoprenyl Phosphate Monosaccharides; D011105:Polyisoprenyl Phosphate Sugars; D011759:Pyrrolidines; D013629:Tamoxifen; D009256:Nafoxidine; D004958:Estradiol