Purification and properties of plant cytochrome b5.
Microsomal cytochrome b5 was 352-fold purified from potato tubers with a yield of 10.4%. To our knowledge, this is the first report relating the purification of higher-plant cytochrome b5. Its Mr (16 700) and absorption spectrum are similar to those of animal and yeast cytochrome b5.
D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003573:Cytochrome b Group / Q000302:isolation & purification*; D015786:Cytochromes b5; D004591:Electrophoresis, Polyacrylamide Gel; D008861:Microsomes; D008970:Molecular Weight; D010944:Plants / Q000032:analysis*; D013053:Spectrophotometry
D003573:Cytochrome b Group; D015786:Cytochromes b5