title: An adenylate cyclase bioassay for parathyroid hormone: some clinical experiences.
abstract: An adenylate cyclase bioassay for parathyroid hormone (PTH) was evaluated in peripheral sera of ten normal subjects, 15 patients with various types of hypoparathyroidism, nine patients with malignancy-associated hypercalcaemia and 60 patients with primary or secondary hyperparathyroidism. The lower limit of detectability was 150 pg/ml in terms of synthetic human PTH (1-34). PTH-like bioactivity was not detected in normal subjects, in patients with surgical hypoparathyroidism, in patients with idiopathic hypoparathyroidism or in patients with malignancy-associated hypercalcaemia. Serum from one untreated pseudohypoparathyroid patient consistently contained PTH-like bioactivity. Bioactivity was detected in sera from 50% of patients with primary or secondary hyperparathyroidism. In two patients with secondary hyperparathyroidism calcium infusions suppressed this activity within 5 min. Both in primary and in secondary hyperparathyroidism there was a significant positive correlation between levels of PTH-like bioactivity, PTH immunoreactivity and the histological severity of the disease.
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D000328:Adult; D001681:Biological Assay / Q000379:methods; D002118:Calcium / Q000097:blood; D006801:Humans; D006934:Hypercalcemia / Q000097:blood; D006961:Hyperparathyroidism / Q000097:blood; D007011:Hypoparathyroidism / Q000097:blood; D007676:Kidney Failure, Chronic / Q000097:blood; D008875:Middle Aged; D010281:Parathyroid Hormone / Q000097:blood*; D011547:Pseudohypoparathyroidism / Q000097:blood
chemical_list: D010281:Parathyroid Hormone; D000262:Adenylyl Cyclases; D002118:Calcium