[Bioluminescent analysis in medicine and biotechnology].
The possible applications of the immobilized bioluminescent systems of bacteria and fireflies in microassay are shown. The immobilization resulted in 10-100-fold stabilization of luciferases permitting their multiple use in flow column reactors. Reagents containing luciferases coimmobilized with other enzymes were used for determination of the picomolar concentrations of ATP, AMP, ADP, NADH2 and NAD+. ATP-metry was used for monitoring the growth of the microorganisms, determination of the biocide effect and estimation of the activity of ATP-dependent enzymes such as creatine kinase in medical diagnosis.
D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D001419:Bacteria / Q000201:enzymology; D001681:Biological Assay / Q000379:methods*; D001517:Coleoptera / Q000201:enzymology; D003402:Creatine Kinase / Q000032:analysis; D004355:Drug Stability; D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D008156:Luciferases / Q000302:isolation & purification / Q000378:metabolism; D008163:Luminescent Measurements; D013677:Medical Laboratory Science; D009243:NAD / Q000032:analysis; D009249:NADP / Q000032:analysis
D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D009243:NAD; D009249:NADP; D000255:Adenosine Triphosphate; D008156:Luciferases; D003402:Creatine Kinase
Bioliuminestsentnyĭ analiz v meditsine i biotekhnologii.