Gas chromatographic identification of Clostridium difficile and detection of cytotoxin from a modified selective medium.
A modification of an existing selective medium for Clostridium difficile is described. Inclusion in the medium of DL nor-leucine and p-hydroxyphenylacetic acid enables identification of C difficile to be made directly from primary isolation plates by gas chromatographic detection of caproic acid and p-cresol. Plugs of agar withdrawn from the selective medium also allow the detection of cytotoxin production in vitro.
D002208:Caproates / Q000032:analysis; D002849:Chromatography, Gas; D003013:Clostridium / Q000302:isolation & purification*; D003408:Cresols / Q000032:analysis; D003470:Culture Media; D003603:Cytotoxins / Q000032:analysis*; D005243:Feces / Q000032:analysis / Q000382:microbiology; D006801:Humans; D009646:Norleucine; D010648:Phenylacetates
D002208:Caproates; D003408:Cresols; D003470:Culture Media; D003603:Cytotoxins; D010648:Phenylacetates; C032538:4-cresol; C008070:4-hydroxyphenylacetic acid; D009646:Norleucine