Purification of a cytotoxic protein produced by the murine macrophage-like cell line J774.1 in response to Sarcophaga lectin.
A tumor specific cytotoxic protein produced by the murine macrophage-like cell line J774.1 in response to stimulation with Sarcophaga lectin was purified to homogeneity in three steps from the culture medium. This cytotoxin, named tumor killing factor (TKF), was a protein with a molecular weight of 15,000, and aggregated forming an oligomer with a molecular weight of 48,000. Its amino acid composition was similar to that of human TNF. Purified TKF had a significant effect on transplanted murine ascites tumor sarcoma 180. The biological significance of TKF in terms of ontogeny is discussed from the view point of developmental biology.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002460:Cell Line; D055598:Chemical Phenomena; D002621:Chemistry; D004175:Diptera; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000096:biosynthesis* / Q000302:isolation & purification; D019476:Insect Proteins; D037102:Lectins / Q000494:pharmacology*; D037181:Lectins, C-Type; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D014409:Tumor Necrosis Factor-alpha
C043030:20kDa lectin protein, Sarcophaga; D000596:Amino Acids; D006023:Glycoproteins; D019476:Insect Proteins; D037102:Lectins; D037181:Lectins, C-Type; D014409:Tumor Necrosis Factor-alpha