Plate binding assay for monoclonal anti-receptor antibodies.
A new procedure has been developed for rapid identification of monoclonal antibodies to the insulin receptor. The technique uses the ability of polyvinyl chloride plates coated with anti-mouse antibodies to specifically adsorb antibodies in hybridoma supernatants. Detection of anti-receptor antibodies is accomplished by allowing the adsorbed anti-receptor antibodies to bind radiolabeled hormone-receptor complexes. The method does not require pure receptor and can be adapted for studies of any receptor or binding protein where a labeled ligand is available. With small amounts of the insulin receptor (20-50 ng), the method could detect antibody concentrations as low as 300 pM. In summary, the assay is sensitive, rapid, and requires small amounts of impure receptor.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D005069:Evaluation Studies as Topic; D007118:Immunoassay / Q000379:methods*; D051379:Mice; D011972:Receptor, Insulin / Q000276:immunology*
D000911:Antibodies, Monoclonal; D011972:Receptor, Insulin