title: Determination of rate constants for the irreversible inhibition of acetylcholine esterase by continuously monitoring the substrate reaction in the presence of the inhibitor.
abstract: The kinetics of the irreversible inhibition of acetylcholinesterase (acetylcholine acetylhydrolase, EC 3.1.1.7) by diisopropyl fluorophosphate and paraoxon have been studied by the approach of following the substrate reaction continuously in the presence of both the substrate and the inhibitor based on kinetic equations previously derived (Tsou, C.-L. (1965) Acta Biochim. Biophys. Sinica 5, 387-417). From determinations of the effects of different concentrations of substrate and the inhibitors on the apparent rate constants for the irreversible inhibition reactions it can be shown that these inhibitors are of the competitive complexing type. Both the reversible dissociation constant for the enzyme inhibitor complex and the rate constant for the subsequent phosphorylation step can be obtained from suitable plots of the experimental data.
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D004557:Electric Organ / Q000201:enzymology; D007531:Isoflurophate / Q000494:pharmacology*; D007700:Kinetics; D008433:Mathematics; D014101:Torpedo
chemical_list: D002800:Cholinesterase Inhibitors; D007531:Isoflurophate; D000110:Acetylcholinesterase