title: In vitro production of metacyclic forms of Trypanosoma musculi and their infectivity in CBA mice.
abstract: Bloodstream forms of Trypanosoma musculi, cultured in Schneider's drosophila medium at room temperature, multiply and differentiate through a series of developmental stages into infective metacyclic trypomastigotes in 10 days. Oral inoculation with these culture forms into CBA mice produced a parasitemia similar to that produced by intraperitoneal infection with bloodstream forms except for a three-day longer prepatent period. Attempts to induce parasitemia with bloodstream forms given orally were unsuccessful.
mesh_terms: D000818:Animals; D003470:Culture Media; D051379:Mice; D008808:Mice, Inbred CBA; D013997:Time Factors; D014345:Trypanosoma / Q000254:growth & development* / Q000472:pathogenicity; D014352:Trypanosomiasis / Q000209:etiology*
chemical_list: D003470:Culture Media