Influence of organic solvents and pH on some characteristics of influenza virus A(H1N1). Note 2. Effect of pH on some spectral and structural characteristics of influenza virus A(H1N1).
Spectrophotometric determinations as regards absorption at 280 nm, 260 nm and 224-237 nm, emission at 280-350 nm and light scattering at 550 nm were performed with suspensions of influenza virus A/Brasil/11/78 (H1N1) over a pH range from 2 to 12. The results obtained proved that changes in spectral parameters were minimal in the pH range from 6 to 10, a fact which could be correlated both with the preservation of the intact virion structure (as visualized by electron microscopy) and with the domain of biological stability of influenza virus.
D006863:Hydrogen-Ion Concentration; D053118:Influenza A Virus, H1N1 Subtype; D009980:Influenza A virus / Q000254:growth & development / Q000648:ultrastructure*; D008027:Light; D008854:Microscopy, Electron; D012542:Scattering, Radiation; D012997:Solvents; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014764:Viral Proteins / Q000032:analysis
D012997:Solvents; D014764:Viral Proteins