Long-term cryopreservation of red cells from patients with sickle cell disease.
Twelve units of red cells (RBCs) from patients with sickle cell disease were glycerolized and frozen for periods of 1 to 3 years. The freeze-thaw hemolysis (mean 11.6% +/- 2.1% SEM) and in vitro process recovery (mean 74.4% +/- 3.7% SEM) were similar to those of sickle RBCs frozen for shorter periods of time. There was a weak positive correlation between duration of frozen storage and cell losses during deglycerolization. The adenosine triphosphate and 2,3-diphosphoglycerate levels of the thawed RBCs were within an acceptable range (mean 3.4 +/- 0.2 mumol/g Hb and 18.0 +/- 0.8 mumol/g Hb, respectively). Long-term cryopreservation of sickle (Hb SS) RBCs opens the possibility of exploring autologous transfusion to treat sickle cell disease patients during anemic episodes that are not due to sickling.
D000755:Anemia, Sickle Cell / Q000097:blood* / Q000628:therapy; D001793:Blood Preservation; D004906:Erythrocyte Count; D017707:Erythrocyte Transfusion; D004912:Erythrocytes / Q000378:metabolism; D005615:Freezing; D005990:Glycerol; D006461:Hemolysis; D006801:Humans; D013997:Time Factors
D005990:Glycerol