title: Testing for the chemical induction of aneuploidy in the male mouse.
abstract: Dyad scores of metaphase II spermatocytes in the mouse have been used as an end point to assess the aneuploidy-inducing potential of three different chemicals; p-fluorophyalanine, phenylalanine and 6-mercaptopurine. The sensitivities of three different spermatogenic stages have been tested; pre-leptotene, zygotene and metaphase I. No effect was found at any treated stage for 6-mercaptopurine and phenylalanine. p-Fluorophenylalanine, when compared to control treatments, did, however, induce non-disjunction when applied at metaphase I. It also caused a delay to spermatogenesis when applied at this stage. The potential of mammalian test systems for the routine screening of chemicals as non-disjunction inducers, is discussed.
mesh_terms: D000782:Aneuploidy; D000818:Animals; D008297:Male; D008540:Meiosis / Q000187:drug effects*; D015122:Mercaptopurine / Q000494:pharmacology*; D008677:Metaphase; D051379:Mice; D009152:Mutagenicity Tests / Q000379:methods*; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D013090:Spermatocytes / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects*; D013997:Time Factors; D010135:p-Fluorophenylalanine / Q000494:pharmacology*
chemical_list: D010649:Phenylalanine; D010135:p-Fluorophenylalanine; D015122:Mercaptopurine