title: Modification of vesicle surfaces with amphiphilic sterols. Effect on permeability and in vivo tissue distribution.
abstract: In this paper, we describe the permeability of vesicles prepared with various synthetic cholesterol derivatives. Cholesterol derivatives with side-chains ending in hydroxyl groups reduced the permeability of unilamellar vesicles. However, addition of cholesterol derivatives with terminal amino groups makes the vesicles more permeable. Vesicles prepared with a short-chain amino-cholesterol derivative were found to be less permeable in phosphate-buffered saline, but not in bovine serum, while long-chain amino-cholesterol-containing vesicles were very permeable in both media. Studies in vivo indicate a rapid clearance rate for intravenously administered amino-cholesterol-containing vesicles with a concomitant increase in liver uptake. However, no difference was found in either the clearance or tissue distribution of control vesicles and the less permeable hydroxyl-cholesterol-containing vesicles.
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D003676:Deferoxamine / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D051379:Mice; D010539:Permeability; D010743:Phospholipids / Q000378:metabolism; D013261:Sterols / Q000378:metabolism*; D013499:Surface Properties; D014018:Tissue Distribution
chemical_list: D010743:Phospholipids; D013261:Sterols; D002784:Cholesterol; D005947:Glucose; D003676:Deferoxamine