An application of fluorescein-conjugated monoclonal antibodies to the diagnosis of Chlamydia trachomatis infection in children.
A method for rapid identification of Chlamydia trachomatis (C. trachomatis) from clinical specimens using fluorescein-conjugated monoclonal antibodies was evaluated. Attempts to isolate C. trachomatis by McCoy cell cultures were simultaneously performed. C. trachomatis was isolated from 8 of 62 newborns with neonatal conjunctivitis in tissue culture as compared with 12 positives using monoclonal antibodies. C. trachomatis was isolated from 2 of 7 infants with pneumonia in tissue cultures as compared with 3 positives using monoclonal antibodies. In order to identify either elementary bodies (EB) or reticulate bodies (RB) in clinical specimens, EB and RB of C. trachomatis L2/434 strain were isolated, purified and reacted with monoclonal antibodies. Majority of fluorescence observed in the clinical specimens was considered to be EB.
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis; D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000276:immunology / Q000302:isolation & purification; D003235:Conjunctivitis, Inclusion / Q000151:congenital / Q000175:diagnosis*; D005452:Fluoresceins; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D011014:Pneumonia / Q000175:diagnosis / Q000209:etiology
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D005452:Fluoresceins