Sequence-specific chemical modification of a 365-nucleotide-long DNA fragment with an alkylating oligonucleotide derivative.
An aromatic 2-chloroethylamino group was attached to the 5'-terminal phosphate of the oligodeoxyribonucleotide pCCCTCTTTCTT. The oligonucleotide derivative prepared was used for modification of the 365-nucleotide-long DNA fragment. It was found that modification of the fragment proceeds in a sequence-specific way at 3 guanosine residues within the sequence complementary to the oligonucleotide reagent.
D000478:Alkylation; D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D003001:Cloning, Molecular; D004247:DNA; D009150:Mustard Compounds; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D010957:Plasmids
D009150:Mustard Compounds; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D004247:DNA