Studies on beta-lactoglobulin as an experimental allergen in rats.
The allergenicity of bovine beta-lactoglobulin (beta-LG) in rats was investigated by measurement of both mast cell-bound and circulating IgE antibody produced in response to immunisation with this cow's milk constituent. The responsiveness of the animals' peritoneal mast cells to in vitro challenge with beta-LG was determined by measurement of release of radiolabelled 5-hydroxytryptamine. Using aluminium hydroxide gel as adjuvant, the responsiveness of mast cells to beta-LG was transient, peaking at 21 days after immunisation. The optimal dose of injected beta-LG was 100 micrograms. Circulating IgE antibody to beta-LG, measured by ELISA, was also detected transiently during the 3rd week post-immunisation.
D000276:Adjuvants, Immunologic; D000485:Allergens; D000536:Aluminum Hydroxide / Q000008:administration & dosage; D000818:Animals; D001666:Binding Sites, Antibody; D004797:Enzyme-Linked Immunosorbent Assay; D007117:Immunization, Secondary; D007073:Immunoglobulin E / Q000276:immunology; D007782:Lactoglobulins / Q000276:immunology*; D008407:Mast Cells / Q000276:immunology / Q000378:metabolism; D051381:Rats; D012701:Serotonin / Q000378:metabolism; D013997:Time Factors
D000276:Adjuvants, Immunologic; D000485:Allergens; D007782:Lactoglobulins; D012701:Serotonin; D007073:Immunoglobulin E; D000536:Aluminum Hydroxide