Sequence-dependent changes in the chiroptical properties of DNA upon interaction with dipyrandium.
Interaction of dipyrandium with DNA and its dependence on the base sequence was studied using circular dichroism. It was found that calf thymus DNA and polynucleotide duplexes with alternating purine-pyrimidine sequences containing GC basepairs underwent similar alterations in the chiroptical properties upon binding of dipyrandium. The alterations suggest that these DNAs have similar B-type structures which may kink at the dipyrandium binding sites. On the other hand, poly(dA-dT).poly(dA-dT) and especially poly(dA-dU).poly(dA-dU) exhibit some features of A-type structure. Poly(dA-dT).poly(dA-dT) changes its chiroptical properties little when complexed with dipyrandium, as if it contained some type of kinks as equilibrium structural elements.
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism*; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D011067:Poly dA-dT / Q000378:metabolism; D011759:Pyrrolidines / Q000494:pharmacology*
D011759:Pyrrolidines; D011067:Poly dA-dT; C004861:dipyrandium; D004247:DNA