Separation of N1- and N8-acetylspermidine isomers by reversed-phase column liquid chromatography after derivatization with dansyl chloride.
The separation of dansyl derivatives of N1- and N8-acetylspermidine by reversed-phase column liquid chromatography is reported. The influence of organic solvents on the retention of acetylspermidines was studied. Best resolutions were achieved using a C18 column and a ternary mobile phase composed of water, methanol and acetonitrile. The precolumn derivatization method permitted the detection of picomole quantities. A method for the determination of acetylspermidines in rat tissues is described.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003619:Dansyl Compounds / Q000032:analysis; D007536:Isomerism; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D011700:Putrescine / Q000031:analogs & derivatives / Q000302:isolation & purification; D051381:Rats; D013095:Spermidine / Q000031:analogs & derivatives* / Q000302:isolation & purification; D013154:Spleen / Q000378:metabolism
D003619:Dansyl Compounds; C030223:N(8)-acetylspermidine; C017988:N(1)-acetylspermidine; C026212:N-acetylputrescine; C029038:dansyl chloride; D013095:Spermidine; D011700:Putrescine