Enzyme-activity mutations detected in mice after paternal fractionated irradiation.
(101/E1 X C3H/E1)F1-hybrid male mice were exposed in a 24-h fractionation interval to either 3.0 + 3.0-Gy or 5.1 + 5.1-Gy X-irradiation, and mated to untreated Test-stock females. The offspring were examined for mutations at 7 recessive specific loci and for activity alterations of erythrocyte enzymes controlled presumably by 12 loci. No enzyme-activity mutant was found in 3610 F1-offspring of the control group. In the experimental groups, no mutant was detected in 533 (3.0 + 3.0 Gy) and 173 (5.1 + 5.1 Gy) offspring from postspermatogonial germ cells treated. After treatment of spermatogonia. I mutant in 3388 F1-offspring of the 3.0 + 3.0-Gy group, and 5 mutants in 3187 F1 offspring of the 5.1 + 5.1-Gy group were found. The mutants were all genetically confirmed. The frequency (expressed as mutants/locus/gamete) of enzyme-activity mutations is 2 (5.1 + 5.1-Gy group) to 10 (3.0 + 3.0-Gy group) times lower than the frequency of recessive specific-locus mutations.
D000818:Animals; D003433:Crosses, Genetic; D004798:Enzymes / Q000235:genetics*; D005260:Female; D005796:Genes / Q000528:radiation effects; D008098:Litter Size / Q000528:radiation effects; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009154:Mutation; D013093:Spermatogonia / Q000528:radiation effects*; D013094:Spermatozoa / Q000528:radiation effects*
D004798:Enzymes