Aromatization of androstenedione by normal and neoplastic endometrium of the uterus.
The ability of human uterine endometrium to aromatize androstenedione to estrogens was investigated using 10 normal and neoplastic tissues. Normal and neoplastic endometrial homogenates were incubated with [6,7-3H]androstenedione (A) and NADPH. Estrone (E1) and estradiol (E2) were subsequently isolated in amounts ranging from 0-17600 fmol/h/g and 0-377 fmol/h/g, respectively, from the incubates after purifications by using Bio-Rad AG1-X2 column, thin layer chromatographies and co-crystallization. The conversion of A to E1 and E2 was significantly higher in neoplastic tissues.
D000328:Adult; D000368:Aged; D000735:Androstenedione / Q000378:metabolism*; D001141:Aromatase / Q000378:metabolism*; D004717:Endometrium / Q000201:enzymology*; D004958:Estradiol / Q000378:metabolism; D004970:Estrone / Q000378:metabolism; D005260:Female; D006801:Humans; D007889:Leiomyoma / Q000201:enzymology; D008598:Menstruation; D008875:Middle Aged; D010060:Ovulation; D010088:Oxidoreductases / Q000378:metabolism*; D014594:Uterine Neoplasms / Q000201:enzymology*
D004970:Estrone; D000735:Androstenedione; D004958:Estradiol; D010088:Oxidoreductases; D001141:Aromatase