Occurrence of adenosine 2',5'-bisphosphate in rat liver.
Adenosine 2',5'-bisphosphate (pAp) is present in liver from 2-day-fasted rats, at a concentration of around 1 microM. pAp was obtained through perchloric acid extraction of the liver followed by two successive DEAE-cellulose chromatographies and an ion-pair high-pressure liquid chromatography. Both pAp extracted from liver and that obtained from a commercial source showed the same pattern of hydrolysis by alkaline phosphatase, i.e., more 5'-AMP than 2'-AMP was obtained as an intermediate of the reaction.
D000244:Adenosine Diphosphate / Q000032:analysis*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D002851:Chromatography, High Pressure Liquid; D008099:Liver / Q000032:analysis*; D008297:Male; D051381:Rats
C045716:adenosine 2',5'-diphosphate; D000244:Adenosine Diphosphate; D000469:Alkaline Phosphatase