title: Time-dependent changes in the binding parameters of muscarinic cholinergic receptors in the rat brain.
abstract: Using quantitative receptor autoradiography, effects of several incubation times on the binding parameters for [3H]quinuclidinyl benzilate [(3H]QNB) binding were investigated in 5 discrete regions of the rat brain. There were no differences in Bmax-values between 3 incubation times. On the other hand, Kd(app)-values markedly depended on the duration of incubation time. Furthermore, Scatchard plots at low [3H]QNB concentrations showed an abnormal binding behavior, i.e. deviation from the straight line representing a single population of the binding site.
mesh_terms: D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000378:metabolism*; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D051381:Rats; D011976:Receptors, Muscarinic / Q000502:physiology*; D013003:Somatosensory Cortex / Q000378:metabolism
chemical_list: D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate