Targeting of enzyme immobilized on erythrocyte membrane to collagen-coated surface.
It is suggested to use 'enzyme(s)-erythrocyte-antibody' complex for modulation of the microenvironment in definite compartments of blood circulation. A model system including peroxidase, human erythrocytes and anti-collagen antibodies was chosen to illustrate the principle. Peroxidase was conjugated to the erythrocyte surface via periodate-oxidized enzyme carbohydrate moiety; biotinylated antibodies were linked by avidin to the biotinylated erythrocytes. The properties of the immunocomplexes obtained have been investigated in an artificial system simulating an injured blood vessel wall. The advantages in using erythrocyte-mediated immunoenzyme complexes for enzyme (drug) targeting are discussed.
D000906:Antibodies; D003094:Collagen; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000201:enzymology*; D004912:Erythrocytes / Q000276:immunology; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D010084:Oxidation-Reduction; D013499:Surface Properties
D000906:Antibodies; D004800:Enzymes, Immobilized; D003094:Collagen; D006735:Horseradish Peroxidase