An experimental model for predicting the synergism of hyperthermia with cytostatics.
A simple prediction test for a combination of hyperthermia and chemotherapy checked on three experimental tumors and cells of the normal murine thymus is described. The testing procedure is based on checking the incorporation of labeled 125I 5-iodo-2-deoxyuridine into the DNA of tested cells affected solely by the enhanced temperature or by the cytostatic itself, or by a combination of both agents. The statistical analysis of repeated results demonstrated the reproducibility of the test. Testing of the potentiation effect of different non-cytostatic substances with enhanced temperatures also gave results corresponding to findings of other authors, performed in vivo or by the use of other techniques. Thus, this simple method is recommended for a screening, before considering the individual use of the combination of hyperthermia and chemotherapy in patients.
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D002460:Cell Line; D003131:Combined Modality Therapy; D004273:DNA, Neoplasm / Q000096:biosynthesis; D006979:Hyperthermia, Induced; D007065:Idoxuridine / Q000378:metabolism; D007939:Leukemia L1210 / Q000628:therapy; D051379:Mice; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D009374:Neoplasms, Experimental / Q000628:therapy*
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D007065:Idoxuridine