title: Injection of diethylstilbestrol on the first day of incubation affects morphology of sex glands and reproductive behavior of Japanese quail.
abstract: Japanese quail eggs were injected with DES (0.9-1,000 micrograms) dissolved in 50-microliter of corn oil on day 1 of incubation. Higher doses of DES (250-1,000 micrograms) reduced hatchability to 37-33% compared to 61% for corn oil-injected controls. Lower doses of DES (0.9-125 micrograms) had no effects on hatchability. In a second study, eggs were injected with 0.9 or 1.9 micrograms of DES and the survivors were assessed up to 12 weeks posthatch. DES did not affect hatchability, but did increase mortality during the first 4 weeks posthatch. Females were affected more than males. At 10 days of age, open-field activity of some birds was examined. The acquisition and reversal of a visual discrimination task was studied at 6 weeks of age. DES had no effect on these measurements. Ten females from each group were chosen randomly to determine egg production over a single 28-day period beginning at 6 weeks of age. Exposure to DES blocked egg production in these birds. The oviduct weights of 12-week-old females were decreased by 50%, but ovarian weights were not affected. Testicular weights were not affected. In a third study reproductive behaviors and social-dominance behaviors of males were markedly attenuated in birds exposed to 0.48 or 1.9 micrograms DES in ovo.
mesh_terms: D000818:Animals; D003370:Coturnix; D004054:Diethylstilbestrol / Q000009:adverse effects*; D004193:Discrimination Learning / Q000187:drug effects; D005260:Female; D005835:Genitalia / Q000033:anatomy & histology* / Q000187:drug effects; D007267:Injections; D009026:Mortality; D009043:Motor Activity / Q000187:drug effects; D009929:Organ Size; D010058:Oviposition / Q000187:drug effects; D010063:Ovum; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D012193:Reversal Learning / Q000187:drug effects; D012726:Sexual Behavior, Animal / Q000187:drug effects*; D012930:Social Dominance
chemical_list: D004054:Diethylstilbestrol