title: Pitfalls in, and new methods for, the radioimmunoassay for digoxin in urine.
abstract: Examining techniques for radioimmunoassay of digoxin in urine, we found that the apparent concentration of digoxin is affected by endogenous substances, independent of the concentration of digoxin itself. We describe a modification of the Becton Dickinson solid-phase RIA that makes it more nearly accurate and more easily performed, and obviates the endogenous interference. Digoxin concentrations from 5.0 to at least 1000 micrograms/L can be measured.
mesh_terms: D004077:Digoxin / Q000652:urine*; D005069:Evaluation Studies as Topic; D006801:Humans; D009994:Osmolar Concentration; D011863:Radioimmunoassay / Q000379:methods; D011933:Reagent Kits, Diagnostic
chemical_list: D011933:Reagent Kits, Diagnostic; D004077:Digoxin