Detection of antithrombin III microheterogeneity.
Antithrombin III microheterogeneity was investigated by isoelectric focusing and immunofixation in healthy individuals and in patients with clinical conditions in which antithrombin III is known to vary (liver disease, nephrotic syndrome, after surgery and anticoagulant therapy). In normal plasma microheterogeneity was present with ten bands of varying intensity being visible in a pI range from 5.0-5.7. One variant was observed which was not associated with a clinical disorder. Low concentrations of antithrombin III were detected in some patients with liver disease, nephrotic syndrome and those on anticoagulant therapy and these demonstrated a decrease of intensity in all bands. Alterations in microheterogeneity were seen in patients tested after surgery and those with nephrotic syndrome. This indicates that changes in the subpopulations of antithrombin III can occur and may be relevant to clinical abnormalities.
D000925:Anticoagulants / Q000494:pharmacology; D000990:Antithrombin III / Q000032:analysis*; D006801:Humans; D007525:Isoelectric Focusing; D008107:Liver Diseases / Q000097:blood; D009404:Nephrotic Syndrome / Q000097:blood; D013514:Surgical Procedures, Operative
D000925:Anticoagulants; D000990:Antithrombin III