Effects of temperature and single-stranded DNA on the interaction of an RNA polymerase III transcription factor with a tRNA gene.
It has previously been shown that a yeast RNA polymerase III transcription factor binds stably to tRNA genes. The principal protein-DNA contacts have now been mapped, by dimethylsulfate footprinting, to the vicinity of the A and B block promoter regions of the S. cerevisiae tRNALeu3 gene. Single-stranded DNA preferentially interferes with the binding of the transcription factor to one of the promoter regions. The effect of temperature on the formation of the transcription factor-DNA complex has been examined: the complex has the kind of "opening" property that has been previously associated with prokaryotic RNA polymerase-promoter complexes, with stability increasing as the temperature is raised.
D004277:DNA, Single-Stranded; D012321:DNA-Directed RNA Polymerases; D008745:Methylation; D011485:Protein Binding; D012320:RNA Polymerase III; D012343:RNA, Transfer / Q000235:genetics*; D013696:Temperature; D014157:Transcription Factors
D004277:DNA, Single-Stranded; D014157:Transcription Factors; D012343:RNA, Transfer; D012321:DNA-Directed RNA Polymerases; D012320:RNA Polymerase III