Assay procedures for cathepsin B, H and L activities in rat tissue homogenates.
Cathepsin B, H and L activities in small amounts of rat tissue homogenates corresponding to 10 micrograms protein were determined with 7-amino-4-methyl-coumarin conjugates as substrates. A new procedure for serum cathepsin H activity was also developed. High cathepsin B and H activities were found in kidney, spleen and liver. Liver cathepsin B, H and L activities in D-galactosamine-injured rats were decreased concomitantly with an increase in serum cathepsin H activity.
D000818:Animals; D002401:Cathepsin B; D056655:Cathepsin H; D056668:Cathepsin L; D002403:Cathepsins / Q000032:analysis* / Q000097:blood; D004796:Clinical Enzyme Tests / Q000379:methods*; D003546:Cysteine Endopeptidases; D010450:Endopeptidases; D005688:Galactosamine / Q000494:pharmacology; D008099:Liver / Q000201:enzymology*; D008107:Liver Diseases / Q000175:diagnosis; D008110:Liver Extracts / Q000032:analysis; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D008110:Liver Extracts; D005688:Galactosamine; D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D002401:Cathepsin B; D056668:Cathepsin L; C534280:Ctsl protein, rat; D056655:Cathepsin H; C534128:Ctsh protein, rat