An ELISA for the detection of maternal anti-trophoblast antibodies in human pregnancy.
An ELISA has been developed which is able to detect antibodies directed against determinants present on the plasma membrane of the outer layer, the syncytiotrophoblast, of term placentae. The IgG and IgM anti-trophoblast antibodies are present in the sera of women during the course of a normal pregnancy. The ELISA was based on the use of syncytiotrophoblast plasma membranes as the antigenic targets and utilised a developing antiserum conjugated to the bacterial enzyme urease. Although the assay was simple and reproducible, its viability was dependent on several factors including the stability of the antibodies and the proper preparative techniques for the production of the plasma membranes. Failure to adhere to the correct procedures resulted in an inferior non-viable assay.
D000418:Albumins / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007518:Isoantibodies / Q000032:analysis*; D007519:Isoantigens / Q000276:immunology; D008431:Maternal-Fetal Exchange; D011247:Pregnancy; D011309:Preservation, Biological; D014327:Trophoblasts / Q000276:immunology*
D000418:Albumins; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D007518:Isoantibodies; D007519:Isoantigens