title: Aromatization of androstenedione by normal and neoplastic endometrium of the uterus.
abstract: The ability of human uterine endometrium to aromatize androstenedione to estrogens was investigated using 10 normal and neoplastic tissues. Normal and neoplastic endometrial homogenates were incubated with [6,7-3H]androstenedione (A) and NADPH. Estrone (E1) and estradiol (E2) were subsequently isolated in amounts ranging from 0-17600 fmol/h/g and 0-377 fmol/h/g, respectively, from the incubates after purifications by using Bio-Rad AG1-X2 column, thin layer chromatographies and co-crystallization. The conversion of A to E1 and E2 was significantly higher in neoplastic tissues.
mesh_terms: D000328:Adult; D000368:Aged; D000735:Androstenedione / Q000378:metabolism*; D001141:Aromatase / Q000378:metabolism*; D004717:Endometrium / Q000201:enzymology*; D004958:Estradiol / Q000378:metabolism; D004970:Estrone / Q000378:metabolism; D005260:Female; D006801:Humans; D007889:Leiomyoma / Q000201:enzymology; D008598:Menstruation; D008875:Middle Aged; D010060:Ovulation; D010088:Oxidoreductases / Q000378:metabolism*; D014594:Uterine Neoplasms / Q000201:enzymology*
chemical_list: D004970:Estrone; D000735:Androstenedione; D004958:Estradiol; D010088:Oxidoreductases; D001141:Aromatase