Erythrocyte heat production in human obesity: microcalorimetric investigation of sodium-potassium pump and cell metabolism.
Changes in overall cellular metabolism, induced by specific inhibition of the Na-K-pump, were determined in erythrocytes from 33 normal and 25 obese subjects. Cellular metabolism was determined by measurement of heat production rates in erythrocytes suspended in plasma with and without the cardioactive glycoside, ouabain. Specific inhibition with ouabain induced the same decrease of the heat production rate in the two groups (14 +/- 5 mW/L for normal subjects and 13 +/- 5 mW/L for obese subjects). In neither group was there a correlation between the ouabain-inhibitable rate of metabolism and body weight. The present study results do not give support to the suggestion that a defect in the Na-K-pump activity would exist in the erythrocyte of human obese subjects and could not therefore be of importance in the pathogenesis of the disease.
D000328:Adult; D001693:Biological Transport, Active / Q000187:drug effects; D002151:Calorimetry / Q000379:methods; D004734:Energy Metabolism / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006358:Hot Temperature; D006801:Humans; D008297:Male; D008832:Microchemistry / Q000379:methods; D008875:Middle Aged; D009765:Obesity / Q000097:blood*; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*
D010042:Ouabain; D012964:Sodium; D011188:Potassium