title: Inhibition of dolichyl phosphate biosynthesis by compactin in cultured rat hepatocytes.
abstract: Isolated rat hepatocytes were cultured in monolayer for about 24 h. During this period the cells exhibited constant protein and lipid synthesis. When the culture medium contained compactin, a competitive inhibitor of the 3-hydroxyl-3-methylglutary-coenzyme-A reductase, dolichyl-P synthesis was inhibited by 91% at the end of the incubation, as estimated by the incorporation of [3H]acetate and by 77% as estimated by the incorporation of 32Pi. These results indicate that in primary cultures of rat hepatocytes dolichyl monophosphate is mainly synthesized through a de novo process, while phosphorylation through the CTP-mediated kinase is of limited functional importance.
mesh_terms: D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000818:Animals; D002478:Cells, Cultured; D002784:Cholesterol / Q000096:biosynthesis; D003570:Cytidine Triphosphate / Q000502:physiology; D004288:Dolichol Phosphates / Q000096:biosynthesis*; D005990:Glycerol / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008148:Lovastatin / Q000031:analogs & derivatives*; D008297:Male; D009281:Naphthalenes / Q000494:pharmacology*; D010743:Phospholipids / Q000096:biosynthesis; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D011106:Polyisoprenyl Phosphates / Q000096:biosynthesis*; D014176:Protein Biosynthesis; D051381:Rats; D014316:Tritium
chemical_list: D000085:Acetates; D004288:Dolichol Phosphates; D009281:Naphthalenes; D010743:Phospholipids; D010761:Phosphorus Radioisotopes; D011106:Polyisoprenyl Phosphates; D014316:Tritium; C028306:dolichol monophosphate; C012258:mevastatin; D003570:Cytidine Triphosphate; D002784:Cholesterol; D008148:Lovastatin; D007930:Leucine; D005990:Glycerol; D019342:Acetic Acid