High-performance liquid chromatographic analysis of 2-aminofluorene and its metabolites in monolayer cultures of rat hepatocytes.
A high-pressure liquid chromatography method was developed to separate 2-aminofluorene and several ring-hydroxylated metabolites. The acetylated derivatives of these compounds and N-hydroxy-2-acetylaminofluorene were also separated simultaneously. HPLC analyses were performed using a Dupont Zorbax C-8 HPLC column and a solvent mixture of 0.02 M acetic acid and isopropanol. Isopropanol concentrations were increased from 27 to 100% over 45 min using a concave gradient system. Desferal mesylate was added to the aqueous component to improve the resolution of several hydroxylated arylamine metabolites. The method was used to quantitate the metabolites of 2-aminofluorene in monolayer cultures of rat hepatocytes.
D015073:2-Acetylaminofluorene / Q000032:analysis; D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005449:Fluorenes / Q000032:analysis* / Q000378:metabolism; D006881:Hydroxyacetylaminofluorene / Q000032:analysis; D008099:Liver / Q000378:metabolism*; D051381:Rats
D005449:Fluorenes; C012177:2-aminofluorene; D006881:Hydroxyacetylaminofluorene; D015073:2-Acetylaminofluorene