title: Column ion exchange chromatographic production of human immune serum globulin for intravenous use.
abstract: Column ion exchange chromatographic processes were developed for the production of immune serum globulin (ISG) for intravenous use. Sequential chromatography on two cross-linked agarose gel anion exchangers yielded the same immunoelectrophoretic pure ISG as a single column DEAE-Sephadex procedure with the advantage of repeated 'in column' cycling. ISG with a low content of aggregated IgG and low anticomplementary activity was prepared from plasma with 53-69% efficiency. The ISG prepared by these processes was characterized with respect to the content of IgA, prekallikrein activator (PKA), plasminogen, fragmentation, and heavy chain subclass distribution.
mesh_terms: D002852:Chromatography, Ion Exchange; D006801:Humans; D007106:Immune Sera / Q000008:administration & dosage / Q000302:isolation & purification*; D007116:Immunization, Passive / Q000009:adverse effects; D007074:Immunoglobulin G / Q000302:isolation & purification; D007275:Injections, Intravenous; D012449:Safety
chemical_list: D007106:Immune Sera; D007074:Immunoglobulin G