[Studies on somatomedin C in diabetic pregnancies].
Somatomedins have been shown to have potent mitogenic activities in cultured cells and also promote individual growth. We studied the concentrations of somatomedin C in diabetic pregnant women and rats. Serum and liver somatomedin C were measured by RIA double antibody method, after extraction with an ODS silica column. Serum somatomedin C concentrations were 20.45 +/- 5.14 nM/l (mean +/- S.D.) in nonpregnant normal women. In nonpregnant diabetic women, these were 16.96 +/- 4.37 nM/l, which were significantly lower than those of normal nonpregnant women. The concentrations of pregnant normal and diabetic women were similar to those of nonpregnant normal women. Maternal concentrations of somatomedin C significantly correlated with infant weight (r = 0.41, p less than 0.05, n = 41). Serum somatomedin C concentrations in the normal infants were 6.45 +/- 1.97 nM/l, which were considerably lower than those of their mothers. In the infants from diabetic mothers, these were 9.17 +/- 3.28 nM/l, which were significantly higher than those of normal infants. Rat serum somatomedin C concentrations which were 15.86 +/- 2.37 nM/l in nonpregnant normal rats, tended to increase during pregnancy. The mother's concentration significantly correlated with fetus weight (r = 0.491). Liver somatomedin C levels decreased in diabetic and pregnant states. Fetal rats had lower hepatic somatomedin C, which was about 10% of the mother's levels. These were higher in the fetuses from diabetic mothers. These finding suggested that the mother's somatomedins might contribute to fetal growth.
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight; D005314:Embryonic and Fetal Development; D005260:Female; D005312:Fetal Blood / Q000032:analysis; D013006:Growth Hormone / Q000097:blood; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000097:blood*; D008099:Liver / Q000033:anatomy & histology; D009929:Organ Size; D011247:Pregnancy; D011254:Pregnancy in Diabetics / Q000097:blood*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013002:Somatomedins / Q000097:blood*
D001786:Blood Glucose; D013002:Somatomedins; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone