Selective loss of a family of gene transcripts in a hereditary murine cataract.
The eye lens of the Fraser mouse contains a dominantly inherited cataract with reduced amounts of seven distinct but homologous gamma crystallins encoded by a family of gamma-crystallin genes. The results of experiments with cultured lenses, cell-free RNA translation, and Northern blot hybridization indicated a specific loss of the family of gamma-crystallin messenger RNA's in the Fraser mouse lens. Southern blot hybridization of genomic DNA's from normal and Fraser mice showed no differences in gamma-crystallin coding sequences.
D000818:Animals; D002386:Cataract / Q000235:genetics*; D003459:Crystallins / Q000235:genetics*; D005796:Genes; D007908:Lens, Crystalline / Q000378:metabolism; D051379:Mice; D008817:Mice, Mutant Strains; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics
D003459:Crystallins; D012333:RNA, Messenger