ADP-ribosylation of metaphase and interphase nonhistones using [3H]adenosine as a radioactive label.
ADP-ribosylation of HeLa nonhistone proteins was investigated by using [3H]adenosine as an in vivo radioactive label. The aim was to determine basic differences in the patterns of modification of interphase and metaphase nonhistones. Fluorography revealed a relatively small number of modified proteins for isolated metaphase chromosomes. In addition to the core histones, a protein of 116 kDa, which is identified as poly-(ADP-ribose) polymerase, was a primary acceptor of [3H]adenosine. Two-dimensional gels revealed a profound difference in the modification of metaphase and interphase nonhistones. For interphase nuclei, 3H label was distributed among a large number of nonhistone acceptors.
D000241:Adenosine / Q000378:metabolism*; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells / Q000166:cytology; D006801:Humans; D007399:Interphase; D008677:Metaphase; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*
D002868:Chromosomal Proteins, Non-Histone; D009702:Nucleoside Diphosphate Sugars; D000246:Adenosine Diphosphate Ribose; D000241:Adenosine