Labelling of retinal capillaries in mice by repeated [3H]thymidine injections.
Injections of [3H]thymidine intraperitoneally to mice on 3 consecutive days label a small number of retinal vascular cells. A single dose of the tracer is not sufficient for adequate cell marking. Retinal capillaries from obese-hyperglycemic mice of different ages examined by this method showed no significant difference of the labelling index compared to their normal, lean controls. The present method is simple and leaves the eyes undisturbed. It may be considered well suitable for experimental studies on cell replication in the retinal capillaries.
D000818:Animals; D002196:Capillaries / Q000166:cytology*; D002455:Cell Division; D007274:Injections, Intraperitoneal; D007553:Isotope Labeling / Q000379:methods*; D051379:Mice; D008820:Mice, Obese; D012171:Retinal Vessels / Q000166:cytology*; D013936:Thymidine / Q000008:administration & dosage*; D014316:Tritium / Q000008:administration & dosage*
D014316:Tritium; D013936:Thymidine