Generation of acetyl glyceryl ether phosphorylcholine from the rat skin and muscle tissues stimulated by moxibustion.
Platelet-activating factor was obtained from the rat skin and muscle tissues which were stimulated by moxibustion. It showed a typical aggregation pattern on interaction with washed rabbit platelets but when it was treated with phospholipases A2 and C, and CV 3988 the aggregation activity was lost. Platelet-activating factor was hydrolysed with phospholipase C and the resulting lipid product was converted to the tert-butyldimethylsilyl derivative. After purification by thin layer chromatography, the ether type of derivative was analysed by a selected ion monitoring technique of gas chromatography-mass spectrometry. 1-0-Hexadecyl-2-acetyl-sn-glyceryl-3-phosphorylcholine only was identified.
D000818:Animals; D002855:Chromatography, Thin Layer; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D009071:Moxibustion; D009132:Muscles / Q000378:metabolism*; D010741:Phospholipases A; D010972:Platelet Activating Factor / Q000096:biosynthesis*; D010974:Platelet Aggregation / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000378:metabolism*; D010738:Type C Phospholipases
D010972:Platelet Activating Factor; D010741:Phospholipases A; D010738:Type C Phospholipases