Potentiation of hybridoma production by the use of mouse fibroblast conditioned media.
L-CM is a conditioned medium prepared from cultures of L-929 cells, a murine fibroblast line. It will promote the growth and antibody secretion of B cell hybridomas after fusion as well as facilitating cloning at limit dilution and the growth of cloned B cell lines in bulk culture. The medium is easy to prepare and stores well at 4 degrees C. It thus provides a convenient alternative to the use of feeder cells in the production of monoclonal antibodies.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002459:Cell Fusion; D002460:Cell Line; D003470:Culture Media; D005347:Fibroblasts / Q000166:cytology; D006133:Growth Substances; D006825:Hybridomas / Q000166:cytology*; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology
D000911:Antibodies, Monoclonal; D003470:Culture Media; D006133:Growth Substances