Continuous-flow analysis for glucose with use of glucose dehydrogenase immobilized in glass tubes.
We describe a method for determining glucose in serum with glucose dehydrogenase immobilized on the inner walls of glass tubes. The reactor was incorporated into a channel of a continuous-flow analyzer (the Technicon SMAC) and used daily for four weeks in routine analysis for serum glucose. During this period we tested the linearity, precision, accuracy, and durability of the reactor. Results correlated well (r = 0.9949) with those obtained by routine methods with free (nonimmobilized) glucose dehydrogenase. The method is shown to be practicable for use in the routine laboratory.
D001322:Autoanalysis; D001786:Blood Glucose / Q000032:analysis*; D002237:Carbohydrate Dehydrogenases; D004800:Enzymes, Immobilized; D005069:Evaluation Studies as Topic; D005898:Glass; D042843:Glucose 1-Dehydrogenase; D005948:Glucose Dehydrogenases; D006801:Humans; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
D001786:Blood Glucose; D004800:Enzymes, Immobilized; D002237:Carbohydrate Dehydrogenases; D005948:Glucose Dehydrogenases; D042843:Glucose 1-Dehydrogenase