title: Identification of a mixture of two different monoclonal antibodies by nonlinear regression analysis after solid phase radioimmunoassay with labeled antigen.
abstract: The results of competitive solid phase radioimmunoassay with labeled antigen, when analyzed by nonlinear regression, may reveal the presence of two different monoclonal antibodies, occasionally produced in a hybridoma culture. In fact, a hybridoma-derived antihuman chorionic somatomammotropin antibody prepared in our laboratories showed an unsatisfactory curve fitting when the experimental data were elaborated on the basis of a monoclonal model. The antibody was subjected to recloning, and gave two separate homogeneous monoclonal antibodies with different affinity constants.
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis*; D000915:Antibody Affinity; D013006:Growth Hormone / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D010928:Placental Lactogen / Q000276:immunology; D011863:Radioimmunoassay; D012044:Regression Analysis
chemical_list: D000911:Antibodies, Monoclonal; D013006:Growth Hormone; D010928:Placental Lactogen