Improved production of oestradiol antibodies of high affinity and specificity by hormonal manipulation of the immunised animals and affinity chromatography of the antisera.
The titre, affinity and specificity of oestradiol antisera were increased by hormonal manipulation of immunised rats. Castration was as effective as daily injection of the aromatase inhibitor, 1,4,6-androstene-3,17-dione. Chromatography on the affinity matrix oestrone-3-CME/Sepharose-4B was less successful.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000915:Antibody Affinity; D000918:Antibody Specificity; D002369:Castration; D002846:Chromatography, Affinity; D004958:Estradiol / Q000276:immunology*; D004970:Estrone / Q000276:immunology; D051381:Rats
D000906:Antibodies; D004970:Estrone; D004958:Estradiol