Identification and preliminary characterization of a sperm-binding protein in normal human semen.
In the seminal plasma of normal men a protein, immunologically related to a major protein (RSV-IV) secreted from the rat seminal vesicle epithelium, was detected by competition with RSV-IV in a specific radioimmunoassay using a rabbit anti RSV-IV antiserum. The protein was partly (80%) purified by column chromatography; characterization by gel electrophoresis indicated that the protein is slightly basic and has a molecular weight of 140 000. The protein was present on the surface of human spermatozoa as well as in the ejaculates of azoospermic men and it is believed to be a sperm-binding protein.
D000954:Antigens, Surface / Q000032:analysis; D002352:Carrier Proteins / Q000302:isolation & purification* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008297:Male; D008970:Molecular Weight; D009845:Oligospermia / Q000378:metabolism; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000276:immunology / Q000378:metabolism; D011863:Radioimmunoassay; D012661:Semen / Q000032:analysis*; D029589:Seminal Plasma Proteins; D013094:Spermatozoa / Q000276:immunology / Q000378:metabolism
D000954:Antigens, Surface; D002352:Carrier Proteins; D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein; C425042:spermadhesin