Elution of anti-Cra. Superiority of the digitonin-acid elution method.
Six examples of IgG anti-Cra were absorbed onto group O, Cr(a+) red cells and subsequently eluted by five elution methods. Five of the anti-Cra antibodies were eluted only by the digitonin-acid method. The sixth example was recovered by all elution methods; however, the digitonin-acid and Lui freeze-thaw methods recovered more antibody activity than did the dichloromethane, ether, or 56 degrees C heat methods. This is the first report of multiple examples of antibodies with the same specificity demonstrated preferentially by a single elution technique.
D000042:Absorption; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D003298:Coombs Test; D004072:Digitonin; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000302:isolation & purification; D007518:Isoantibodies / Q000302:isolation & purification*
D007074:Immunoglobulin G; D007518:Isoantibodies; D004072:Digitonin