Clonal origin of human basophil/mast cells from circulating multipotent hemopoietic progenitors.
The origin of the human basophil/mast cell lineage from a pluripotent hematopoietic stem cell has been surmised but never demonstrated. By examining individual hemopoietic colonies in methylcellulose under inverted microscopy and using histochemical stains in conjunction with single-colony histamine assays, we have previously identified basophil/mast cell progenitors in human peripheral blood. We now report that a large proportion of normal human peripheral blood mixed granuloerythropoietic (GEMM) colonies contain histamine, in contrast to a significantly lower frequency of histamine positivity among normal neutrophil-macrophage, eosinophil, erythroid, macrophage, or megakaryocyte colonies. Morphological observations confirmed the presence of basophil/mast cells in the majority of GEMM colonies. In our work, the clonal derivation of basophils/mast cells from circulating multipotent (CFU-GEMM) hemopoietic stem cells was formally demonstrated, using combined histamine and G6PD isoenzyme analysis of single colonies grown in methylcellulose from a normal G6PD heterozygote.
D001491:Basophils / Q000166:cytology*; D002999:Clone Cells / Q000166:cytology; D003114:Colony-Forming Units Assay; D004912:Erythrocytes / Q000166:cytology; D006580:Genetic Carrier Screening; D005954:Glucosephosphate Dehydrogenase / Q000097:blood / Q000235:genetics; D006098:Granulocytes / Q000166:cytology / Q000378:metabolism; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006632:Histamine / Q000097:blood; D006801:Humans; D007527:Isoenzymes / Q000097:blood / Q000235:genetics; D008407:Mast Cells / Q000166:cytology*
D007527:Isoenzymes; D006632:Histamine; D005954:Glucosephosphate Dehydrogenase