title: Improved production of oestradiol antibodies of high affinity and specificity by hormonal manipulation of the immunised animals and affinity chromatography of the antisera.
abstract: The titre, affinity and specificity of oestradiol antisera were increased by hormonal manipulation of immunised rats. Castration was as effective as daily injection of the aromatase inhibitor, 1,4,6-androstene-3,17-dione. Chromatography on the affinity matrix oestrone-3-CME/Sepharose-4B was less successful.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000915:Antibody Affinity; D000918:Antibody Specificity; D002369:Castration; D002846:Chromatography, Affinity; D004958:Estradiol / Q000276:immunology*; D004970:Estrone / Q000276:immunology; D051381:Rats
chemical_list: D000906:Antibodies; D004970:Estrone; D004958:Estradiol