Ultrathin-layer polyacrylamide gel isoelectric focusing for the identification of hemoglobin variants.
An ultrathin-layer polyacrylamide gel isoelectric focusing method for typing hemoglobin is described. This method, which uses Pharmalyte pH 6.7-7.7 and an electrode wick distance of 5.0 cm, clearly resolves the A, F, S, C and a number of rare variant allelic products. The technique is ideal for rapid screening of a large number of fresh blood and/or bloodstained samples.
D001797:Blood Protein Electrophoresis / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005006:Ethnicity; D005554:Forensic Medicine / Q000379:methods; D006452:Hemoglobinometry / Q000379:methods*; D006455:Hemoglobins, Abnormal / Q000032:analysis*; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods*; D008403:Mass Screening; D044469:Racial Groups
D006455:Hemoglobins, Abnormal