Two examples of low ionic strength-dependent autoagglutinins with anti-Pr1 specificity.
Two low ionic strength-dependent autoagglutinins were studied and found to have anti-Pr1 specificity. This specificity was determined by studies with enzyme-treated and neuraminidase-treated human red blood cells (RBCs), animal RBCs and chemically-modified sialoglycoproteins, all suspended in a low ionic strength solution (LISS). Both IgM complement-binding cold agglutinins had a wide thermal range and caused in vitro hemolysis of some LISS-suspended RBCs at 37 degrees C. Compatible blood was found for these patients by using techniques that did not employ LISS.
D000372:Agglutination Tests; D000918:Antibody Specificity; D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology; D001789:Blood Group Antigens; D003080:Cold Temperature; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009439:Neuraminidase / Q000494:pharmacology; D009994:Osmolar Concentration; D012795:Sialoglycoproteins / Q000494:pharmacology
D001323:Autoantibodies; D001789:Blood Group Antigens; D012795:Sialoglycoproteins; D009439:Neuraminidase