A simple and rapid method for sequencing DNA.
A simplified technique for DNA sequence analysis has been developed, based on modification of a previous method [(1980) Methods Enzymol. 65, 499-560]. It employs an adsorptive immobilization of terminally labelled DNA on DEAE paper followed by G, A+G, C+T and C specific modification and cleavage reactions. This solid-phase technique is faster and more convenient than the original method. The efficiency is comparable. The total processing time taken to produce cleaved fragments loaded on a gel is less than 2 h.
D001345:Autoradiography; D001483:Base Sequence; D002854:Chromatography, Paper / Q000379:methods; D004247:DNA; D006868:Hydrolysis; D010761:Phosphorus Radioisotopes; D010957:Plasmids
D010761:Phosphorus Radioisotopes; D004247:DNA