Inhibitory effect of cytochalasins on the motility of rat epididymal spermatozoa in vitro.
Cytochalasins A, B, C, D and E at a concentration of 5.0 microgram/ml significantly inhibited the motility of spermatozoa collected from the cauda epididymidis of rats when diluted in Hank's solution containing BSA by 21.6-38.1% within a few min incubation. The motility was significantly inhibited (approx. 16.7%) by Cyto. E at a concentration as low as 1.0 microgram/ml within 30 min. This inhibitory effect of Cyto. E was a dose-dependent at the concentrations ranging from 1.0-20.0 microgram/ml. These findings demonstrate that cytochalasins are powerful inhibitors on the motility of rat epididymal spermatozoa.
D000818:Animals; D001681:Biological Assay; D003571:Cytochalasin B / Q000494:pharmacology; D015638:Cytochalasin D; D003572:Cytochalasins / Q000032:analysis / Q000494:pharmacology*; D004822:Epididymis / Q000166:cytology / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D013081:Sperm Motility / Q000187:drug effects*
D003572:Cytochalasins; C031218:cytochalasin A; C031582:cytochalasin C; D015638:Cytochalasin D; C007215:cytochalasin E; D003571:Cytochalasin B