The use of monoclonal antibodies for the species and tissues distribution of phospholamban.
Monoclonal antibodies have been raised against canine phospholamban. Two antibodies have been used in the study of phospholamban distribution in tissues, and in different animal species. The antibodies recognized the three different forms of phospholamban: non-phosphorylated, phosphorylated, and dissociated forms. A survey of nine rabbit tissues revealed that phospholamban is a cardiac muscle specific protein. Phospholamban from different mammalian hearts were found to be identical with respect to molecular weight and antigenicity.
D000818:Animals; D000911:Antibodies, Monoclonal; D001345:Autoradiography; D002135:Calcium-Binding Proteins / Q000032:analysis* / Q000276:immunology; D002417:Cattle; D003101:Collodion; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D007120:Immunochemistry; D051379:Mice; D008970:Molecular Weight; D009206:Myocardium / Q000032:analysis*; D009928:Organ Specificity; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D000099211:Phospholamban
D000911:Antibodies, Monoclonal; D002135:Calcium-Binding Proteins; D003101:Collodion; D000099211:Phospholamban