Visualization of the secretory canaliculi of human parietal cells with a peroxidase-labelled peanut lectin. Light- and electron-microscopic observations.
Peanut lectin reactivity was examined in normal fundic glands from human gastric samples, both at light- and electron-microscopic levels, using a peroxidase conjugate. Positive reaction was observed in the glycocalyx of parietal cell secretory canaliculi as well as in the mucous globules of mucous cells and in the luminal cell coat of chief cells. The presence of terminal galactose in the canalicular glycocalyx may be connected with the peculiar function of hydrochloric acid secretion. Peroxidase-labelled peanut lectin is proposed as a marker for visualizing the secretory canaliculus of parietal cells.
D005690:Galactose / Q000378:metabolism; D006801:Humans; D037102:Lectins; D008854:Microscopy, Electron; D010295:Parietal Cells, Gastric / Q000378:metabolism / Q000648:ultrastructure*; D019887:Peanut Agglutinin
D037102:Lectins; D019887:Peanut Agglutinin; D005690:Galactose