Abrogation of macrophage-mediated suppression of T-lymphocyte proliferation with hemoglobin and hemin is a function of iron content.
Hemoglobin, hemin, and ferric ion (Fe) were shown to reverse peritoneal exudate cell (PEC)-mediated suppression of concanavalin A-elicited murine spleen cell activation. Titration of hemoglobin and hemin relative to Fe showed a direct relationship between Fe content and reversal of PEC suppression. Indomethacin enhanced the capacity of all three compounds to abrogate PEC suppression on the order of five- to eight-fold. The capacity of endogenous Fe-containing substances as hemoglobin and its catabolites, e.g., hemin, to modulate macrophage expression may be of special significance at sites of inflammation.
D000818:Animals; D006418:Heme / Q000031:analogs & derivatives*; D006427:Hemin / Q000502:physiology*; D006454:Hemoglobins / Q000502:physiology*; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007108:Immune Tolerance; D007213:Indomethacin / Q000494:pharmacology; D007501:Iron / Q000097:blood* / Q000494:pharmacology; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010529:Peritoneal Cavity / Q000166:cytology; D013154:Spleen / Q000166:cytology
D006454:Hemoglobins; D006418:Heme; D006427:Hemin; D007501:Iron; D007213:Indomethacin