Biological and biochemical properties of nerve growth factor synthesized by mouse S-180 cells in culture.
Nerve Growth Factor (NGF), a protein involved in the maintenance and differentiation of sensory and sympathetic neuronal cells [1], is synthesized by several different types of cells in culture [2-7]. In this paper, the biochemical and biological properties of NGF synthesized by a mouse S-180 sarcoma cell line were examined. These cells do not appear to produce the 7S-NGF molecule, a form of NGF found in high concentrations in the mouse submandibular gland [8]. The 7S-NGF is comprised of three distinct protein subunits named beta-NGF, alpha and gamma [9]. Although the S-180 cells do not produce 7S-NGF, the cells do synthesize one of the component subunits of 7S-NGF, the beta-NGF subunit. Biological, electrophoretic, immunological and molecular weight criteria were used to establish that the beta-NGF synthesized by the S-180 cells is very similar to the submandibular gland beta-NGF. The S-180 beta-NGF was bound to an unidentified binding component(s) which is not immunologically similar to either the alpha- or gamma-subunit. The functional significance of this interaction is not known.
D000818:Animals; D001681:Biological Assay; D002460:Cell Line; D002642:Chick Embryo; D002850:Chromatography, Gel; D003470:Culture Media; D007526:Isoelectric Point; D051379:Mice; D008970:Molecular Weight; D009412:Nerve Fibers / Q000166:cytology / Q000187:drug effects; D009414:Nerve Growth Factors / Q000032:analysis / Q000096:biosynthesis* / Q000494:pharmacology; D011863:Radioimmunoassay; D012510:Sarcoma 180 / Q000032:analysis / Q000378:metabolism*; D013363:Submandibular Gland / Q000032:analysis
D003470:Culture Media; D009414:Nerve Growth Factors