Determination of serum aspartate aminotransferase with pyridoxal 5'-phosphate in the Technicon SMAC and Du Pont aca compared and correlated with the IFCC recommended method.
Inexpensive improvements in continuous-flow analytical apparatus can eliminate some perplexing inconsistencies and limitations in the use of the Technicon SMAC instrument for measuring the activity in serum of aspartate aminotransferase (EC 2.6.1.1) in the presence of pyridoxal 5'-phosphate. In addition, a calibration procedure based on values for this enzyme obtained by a modified IFCC method can be used to calibrate both the Technicon SMAC and the Du Pont aca instruments to produce excellent correlation between the two.
D001219:Aspartate Aminotransferases / Q000097:blood*; D001322:Autoanalysis; D002138:Calibration; D006801:Humans; D007656:Ketoglutaric Acids; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D009243:NAD; D011732:Pyridoxal Phosphate; D013056:Spectrophotometry, Ultraviolet
D007656:Ketoglutaric Acids; D009243:NAD; D011732:Pyridoxal Phosphate; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D001219:Aspartate Aminotransferases