Characterization and crystallization of delta-crystallin from the eye lens of adult turkey: comparison with delta-crystallin from adult quail and young chick.
delta-Crystallin, purified from either adult turkey or quail lenses, and characterized by amino acid composition and circular dichroism spectroscopy, is shown to be similar to delta-crystallin purified from 3-day-old chick lenses. Crystallization of turkey delta-crystallin is reported along with the characterization of the crystal unit cell by X-ray diffraction techniques. Variation in unit cell parameters is observed and is correlated with hydration.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002645:Chickens; D002942:Circular Dichroism; D003459:Crystallins / Q000302:isolation & purification* / Q000528:radiation effects; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D007908:Lens, Crystalline / Q000032:analysis*; D011784:Quail; D014364:Tryptophan / Q000032:analysis; D014422:Turkeys; D014961:X-Ray Diffraction
D000596:Amino Acids; D003459:Crystallins; D014364:Tryptophan