Localization of the amino acid substitution site in a fast migrating variant of human serum albumin.
Albumin Mi/Fg is an Italian genetic variant of human serum albumin arising from a Lys----Glu substitution which has been located in a CNBr fragment (CNBr VII) corresponding to the -COOH terminal portion of the molecule [(1984) J. Chromatogr. 298, 336-344]. Tryptic peptides of CNBr VII from normal and Mi/Fg albumin have been purified by reverse-phase high-performance liquid chromatography (RP-HPLC) and submitted to comparative structural studies. The amino acid sequence of the tryptic peptide of Mi/Fg variant that differs from the corresponding fragment of the normal serum albumin shows that the Lys----Glu substitution responsible for this variant is located at position 573. This region of the albumin molecule is involved in the binding of long chain fatty acids.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003488:Cyanogen Bromide; D004586:Electrophoresis; D005227:Fatty Acids / Q000378:metabolism; D006801:Humans; D010446:Peptide Fragments / Q000032:analysis; D012709:Serum Albumin / Q000032:analysis* / Q000235:genetics; D014357:Trypsin / Q000378:metabolism
D000596:Amino Acids; D005227:Fatty Acids; D010446:Peptide Fragments; D012709:Serum Albumin; D014357:Trypsin; D003488:Cyanogen Bromide