title: Protein mixed-disulfides in cardiac cells. S-thiolation of soluble proteins in response to diamide.
abstract: Protein mixed-disulfides in cultured rat heart cells were analyzed by gel electrophoresis under conditions that eliminated artifactual formation of these protein derivatives. Protein S-thiolation (protein mixed-disulfide formation) was detectable under normal culture conditions. Diamide oxidized intracellular glutathione in these cells and produced extensive protein S-thiolation. The specificity of this protein modification indicates a role in the regulation of cardiac metabolism.
mesh_terms: D000818:Animals; D001391:Azo Compounds / Q000494:pharmacology*; D003553:Cystine / Q000378:metabolism; D003958:Diamide / Q000494:pharmacology*; D004220:Disulfides; D005978:Glutathione / Q000378:metabolism; D008970:Molecular Weight; D009124:Muscle Proteins; D009206:Myocardium / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats
chemical_list: D001391:Azo Compounds; D004220:Disulfides; D009124:Muscle Proteins; D003958:Diamide; D003553:Cystine; D005978:Glutathione