Determination of protein in polysaccharide-antibody complexes.
We describe a simple and sensitive method for determining antibody protein complexed with polysaccharide using binding of Amido-black to antigen-antibody complexes and bovine serum albumin as protein standard. The method, which is a modification of the method used for the determination of total nitrogen or the protein concentration in milk, has shown a consistent quantitative relationship with the Lowry procedure and has been developed for its particular application to serological precipitates.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000936:Antigen-Antibody Complex; D002417:Cattle; D005260:Female; D008894:Milk Proteins / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D011425:Propionibacterium acnes / Q000276:immunology*; D011506:Proteins / Q000032:analysis*; D012710:Serum Albumin, Bovine
D000906:Antibodies; D000936:Antigen-Antibody Complex; D008894:Milk Proteins; D011135:Polysaccharides, Bacterial; D011506:Proteins; D012710:Serum Albumin, Bovine