Protein/platelet interaction with an artificial surface: effect of vitamins and platelet inhibitors.
Protein adsorption and platelet adhesion are two important biological processes arising at the blood-prosthetic interface. The effect of Vitamins and antiplatelet drugs to modulate the surface induced platelet adhesion to polycarbonate was investigated using washed calf platelets in presence and absence of fibrinogen. This study also demonstrated the effects of Vitamins and antiplatelet drugs towards protein adsorption to an artificial surface. It seems Vitamin B6, Vitamin E, combinations of Aspirin-Persantine, Aspirin-Vitamin C, a synthetic Polyelectrolyte and Galactosamine reduced the fibrinogen (fg) surface concentration from a mixture of proteins. These antiplatelet agents also enhanced the albumin surface concentration. This itself may be one of the parameters to reduce the platelet adhesion towards an artificial surface. A combination of Aspirin-Vitamin C-Vitamin B6-Vitamin E inhibited the fibrinogen surface binding, which might be beneficial to improve the blood compatibility of an artificial surface.
D000327:Adsorption; D000606:Amino Sugars / Q000494:pharmacology; D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects; D001798:Blood Proteins / Q000378:metabolism*; D002417:Cattle; D004176:Dipyridamole / Q000494:pharmacology; D005340:Fibrinogen / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D010973:Platelet Adhesiveness / Q000187:drug effects*; D011076:Polycarboxylate Cement; D013927:Thrombosis / Q000209:etiology; D014815:Vitamins / Q000494:pharmacology*
D000606:Amino Sugars; D001798:Blood Proteins; D011076:Polycarboxylate Cement; D014815:Vitamins; C005506:polycarbonate; D004176:Dipyridamole; D005340:Fibrinogen; D001241:Aspirin