Transformation of human bronchial epithelial cells transfected by Harvey ras oncogene.
Transfection of normal human bronchial epithelial (NHBE) cells with a plasmid carrying the ras oncogene of Harvey murine sarcoma virus (v-Ha ras) changed the growth requirements, terminal differentiation, and tumorigenicity of the recipient cells. One of the cell lines isolated after transfection (TBE-1) was studied extensively and shown to contain v-Ha ras DNA. Total cellular RNA from TBE-1 cells hybridized to v-Ha ras structural gene fragment probes five to eight times more than RNA from parental NHBE cells. The TBE-1 cells expressed phosphorylated v-Ha ras polypeptide p21, showed a reduced requirement for growth-factor supplements, and became aneuploid as an early cellular response to v-Ha ras expression. As the transfectants acquire an indefinite life-span and anchorage independence they became transplantable tumor cells and showed many phenotypic changes suggesting a pleiotropic mechanism for the role of Ha ras in human carcinogenesis.
D000818:Animals; D001980:Bronchi / Q000166:cytology* / Q000382:microbiology; D002283:Carcinoma, Bronchogenic / Q000235:genetics; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000378:metabolism; D002472:Cell Transformation, Viral; D003470:Culture Media; D004273:DNA, Neoplasm / Q000235:genetics; D004847:Epithelial Cells; D004848:Epithelium / Q000382:microbiology; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics; D051379:Mice; D008819:Mice, Nude; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D051381:Rats; D014162:Transfection
D003470:Culture Media; D004273:DNA, Neoplasm