title: Prostaglandin requirements are greater for protection in cold restraint-induced than alcohol-induced gastric mucosal injury.
abstract: Exogenous prostaglandins inhibit visible gastric mucosal lesions produced by both absolute ethanol and cold restraint in the rat. Pretreatment with "mild irritants" significantly reduces the magnitude of ethanol-induced lesions presumably by stimulating endogenous prostanoid production. The effect of mild irritant pretreatment on cold restraint-induced lesion formation has not been previously reported. This study was designed to compare the protective effect of pretreatment with two "mild irritants," 4% NaCl and 0.35 M HCl, and the synthetic prostanoid, 16,16 dimethyl PGE2(16,16-dm PGE2), on lesions produced by cold restraint or absolute ethanol. Pretreatment with both mild irritants produced complete visible protection against ethanol-induced injury but had variable effects against cold restraint-induced injury. Whereas 5 micrograms/kg 16,16-dmPGE2 provided complete visible protection against ethanol-induced injury, 20 micrograms/kg 16,16-dmPGE2 was required for complete visible protection against cold restraint-induced injury. We conclude that prostaglandin requirements for protection against cold restraint injury are greater than for protection against ethanol-induced gastric mucosal injury.
mesh_terms: D015064:16,16-Dimethylprostaglandin E2 / Q000494:pharmacology; D000818:Animals; D003080:Cold Temperature; D000431:Ethanol / Q000037:antagonists & inhibitors*; D005753:Gastric Mucosa / Q000187:drug effects / Q000473:pathology*; D006851:Hydrochloric Acid / Q000494:pharmacology; D007509:Irritants / Q000494:pharmacology; D008297:Male; D009336:Necrosis / Q000139:chemically induced / Q000209:etiology / Q000517:prevention & control; D011453:Prostaglandins / Q000494:pharmacology* / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D012149:Restraint, Physical; D012462:Saline Solution, Hypertonic / Q000494:pharmacology
chemical_list: D007509:Irritants; D011453:Prostaglandins; D012462:Saline Solution, Hypertonic; D000431:Ethanol; D015064:16,16-Dimethylprostaglandin E2; D006851:Hydrochloric Acid