Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-bisphosphate carboxylase.
Chimaeric genes can be constructed which fuse the transit peptide of a small subunit of the chloroplast-located ribulose 1,5-bisphosphate carboxylase with a bacterial protein. The fusion protein is translocated into chloroplasts and cleaved in a similar way to the small subunit polypeptide precursor.
D000595:Amino Acid Sequence; D001483:Base Sequence; D001693:Biological Transport, Active; D002451:Cell Compartmentation; D002736:Chloroplasts / Q000378:metabolism*; D046911:Macromolecular Substances; D008970:Molecular Weight; D010944:Plants / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics / Q000378:metabolism*
D046911:Macromolecular Substances; D012273:Ribulose-Bisphosphate Carboxylase