Colon adenocarcinoma-associated nuclear antigens.
Colon adenocarcinoma-associated chromosomal nonhistone proteins were determined in rat colon adenocarcinoma chromatin and in colon chromatin of Sprague-Dawley rats treated with 1,2-dimethylhydrazine (DMH) by the enzyme-linked immunosorbent assay method. The specific nuclear proteins are tissue-specific oncofetal antigens. They were also detected in fetal rat colon chromatin but not in adult rat colon chromatin. The specific tumor-associated nonhistone proteins were identified by immunoblot. The molecular weight ranges of these proteins were 79,000-89,000 and 40,000-56,000 daltons. Administration of DMH to Sprague-Dawley rats induced colon tumor and colon tumor-associated nonhistone proteins in the chromatin. However, DMH failed to induce colon tumor and tumor-associated nonhistone proteins in Lobund-Wistar rats.
D000230:Adenocarcinoma / Q000276:immunology*; D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D002467:Cell Nucleus / Q000276:immunology*; D002843:Chromatin / Q000276:immunology*; D003110:Colonic Neoplasms / Q000276:immunology*; D004127:Dimethylhydrazines; D005260:Female; D007668:Kidney / Q000276:immunology; D008099:Liver / Q000276:immunology; D008970:Molecular Weight; D009374:Neoplasms, Experimental / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains
D000951:Antigens, Neoplasm; D002843:Chromatin; D004127:Dimethylhydrazines