title: Influence of organic solvents and pH on some characteristics of influenza virus A(H1N1). Note 2. Effect of pH on some spectral and structural characteristics of influenza virus A(H1N1).
abstract: Spectrophotometric determinations as regards absorption at 280 nm, 260 nm and 224-237 nm, emission at 280-350 nm and light scattering at 550 nm were performed with suspensions of influenza virus A/Brasil/11/78 (H1N1) over a pH range from 2 to 12. The results obtained proved that changes in spectral parameters were minimal in the pH range from 6 to 10, a fact which could be correlated both with the preservation of the intact virion structure (as visualized by electron microscopy) and with the domain of biological stability of influenza virus.
mesh_terms: D006863:Hydrogen-Ion Concentration; D053118:Influenza A Virus, H1N1 Subtype; D009980:Influenza A virus / Q000254:growth & development / Q000648:ultrastructure*; D008027:Light; D008854:Microscopy, Electron; D012542:Scattering, Radiation; D012997:Solvents; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014764:Viral Proteins / Q000032:analysis
chemical_list: D012997:Solvents; D014764:Viral Proteins