Fast hemoglobins and red blood cell metabolites in citrate phosphate dextrose adenine stored blood.
Blood was drawn from 10 fasted, healthy volunteers and stored under standard blood bank conditions in citrate-phosphate-dextrose-adenine (CPDA-1). Blood was sampled before storage (Day 0) and on Days 5, 12, 19, 26, and 35. Laboratory testing for glucose, HbAla + b, HbAlc, pyruvic acid, lactic acid, adenosine triphosphate (ATP), 2, 3-diphosphoglycerate (2,3DPG), plasma free hemoglobin (Hb) and pH (blood gases) were performed. In addition, P50 was also serially measured in two of the individuals and in their stored blood. Significant elevations of HbAla + b and HbAlc (fast hemoglobins) were found on Days 12 and 19 of storage (p less than 0.05). These elevations of fast hemoglobins are due to hypoxia, acidosis, and hyperglycemia. Following the initial elevation of the fast hemoglobins (Hbs), there was a decline in their concentration, from Day 12, which could partly be explained by cell death.
D000225:Adenine / Q000032:analysis*; D001793:Blood Preservation; D002851:Chromatography, High Pressure Liquid; D002951:Citrates / Q000032:analysis*; D004912:Erythrocytes / Q000378:metabolism*; D005947:Glucose / Q000032:analysis*; D006442:Glycated Hemoglobin / Q000032:analysis; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D010710:Phosphates / Q000032:analysis*
D000225:Adenine; D002951:Citrates; D005947:Glucose; D006442:Glycated Hemoglobin; D006454:Hemoglobins; D010710:Phosphates; C034876:CPDA solutions; C020622:hemoglobin B