Persistence of echovirus 6 in cloned human cells.
Establishment of a persistent infection by echovirus 6 in cloned human WISH cells (PI) was demonstrated. The cloned human WISH cells were maintained for 3.0 years (over 125 passages) and released virus continuously without cellular destruction. Neither temperature-sensitive virus mutants nor interferon appears to play a role in either establishment or maintenance of viral persistence. The majority of the virus produced by cloned human WISH cells is defective (2 X 10(6) particles per PFU) and differs from standard virus in its polypeptide profile and its inability to attach to parental WISH cells.
D002478:Cells, Cultured / Q000382:microbiology*; D002999:Clone Cells / Q000382:microbiology; D003673:Defective Viruses / Q000032:analysis / Q000254:growth & development; D004458:Echovirus 6, Human / Q000254:growth & development*; D004457:Echovirus Infections / Q000382:microbiology*; D029822:Enterovirus B, Human / Q000254:growth & development*; D006801:Humans; D013696:Temperature; D014764:Viral Proteins / Q000032:analysis; D014779:Virus Replication
D014764:Viral Proteins