Detection of sugar-binding proteins in membrane-depleted nuclei.
Nuclear sugar-binding proteins were detected in membrane-depleted nuclei isolated from hamster BHK cells and mouse L 1210 leukemia cells by means of fluorescein-labelled neoglycoproteins. In fluorescence microscopy, the fluorescence was seen throughout the nucleus but was generally brighter over the nucleoli than over the rest of the nucleus. Flow cytofluorometry analysis demonstrated the presence of nuclear sugar-binding proteins for synthetic glycoproteins associated with different sugar residues. Among the nine neoglycoproteins used, four neoglycoproteins (namely alpha-rhamnosylated, alpha-glucosylated, N-acetyl-beta-glucosaminylated and alpha-mannosylated-6P-serum albumin) strongly labelled nuclei. Various controls strongly argue for the specificity of the nuclear labelling. The possibility that some of the sugar-binding proteins might correspond to endogenous nuclear lectins is considered.
D000818:Animals; D002460:Cell Line; D002466:Cell Nucleolus / Q000032:analysis / Q000378:metabolism; D002467:Cell Nucleus / Q000032:analysis* / Q000378:metabolism; D006224:Cricetinae; D005434:Flow Cytometry; D006023:Glycoproteins / Q000378:metabolism; D007668:Kidney; D037102:Lectins / Q000032:analysis*; D007939:Leukemia L1210; D008856:Microscopy, Fluorescence
D006023:Glycoproteins; D037102:Lectins