A novel magnesium-dependent structural transition of chicken erythrocyte chromatin in situ.
Lowering magnesium concentration below the value of 1 mM leads to a structural transition of chicken erythrocyte chromatin in situ, which results in a change in its fragmentation by pancreatic DNAase (DNAase I) from double-nucleosome to 100-basepairs mode. At 0.75 mM MgCl2, the pattern of chromatin fragmentation by DNAase I is similar to that generated by DNAase II, and it is further changed at lower concentrations of magnesium. This transition is, at least partly, reversible, and is, presumably, related to packing of the 25-30 nm chromatin fiber into higher-order structures.
D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000187:drug effects*; D003850:Deoxyribonuclease I / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004912:Erythrocytes / Q000032:analysis; D008274:Magnesium / Q000494:pharmacology*
D002843:Chromatin; D003851:Deoxyribonucleases; D003850:Deoxyribonuclease I; D008274:Magnesium