Evaluation of absorption and first- and second-derivative spectra for simultaneous quantification of bilirubin and hemoglobin.
We discuss the relative merits of absorption and first- and second-derivative spectra for the simultaneous quantification of bilirubin and hemoglobin, and evaluate single-, two-, and multiwavelength methods. Although both species can be quantified from single- or two-wavelength absorption data, lipids or other absorbing or light-scattering components introduce systematic errors that can be substantially decreased by using first- or second-derivative spectra. Multi-wavelength data-processing methods with derivative spectra permit quantification of components with overlapping spectra and decrease the random error usually associated with derivative methods. A typical least-squares equation for quantifying bilirubin in the presence of hemoglobin and bovine serum albumin from multi-wavelength second-derivative data is y(computed) = 0.999x(prepared) + 0.00 mg/L.
D001663:Bilirubin / Q000032:analysis*; D005069:Evaluation Studies as Topic; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D008055:Lipids / Q000032:analysis; D008838:Microcomputers; D012044:Regression Analysis; D012710:Serum Albumin, Bovine / Q000032:analysis; D013053:Spectrophotometry / Q000379:methods
D006454:Hemoglobins; D008055:Lipids; D012710:Serum Albumin, Bovine; D001663:Bilirubin