Selective detection of Mycoplasma hyorhinis using cloned genomic DNA fragments.
A 13-kilobase DNA fragment from a genomic library of Mycoplasma hyorhinis demonstrated specific Southern hybridization and dot hybridization when tested against a group of different mycoplasmas. This probe selectively recognized M. hyorhinis sequences in purified DNA, broth-grown organisms, and infected cell cultures, providing a direct method for differential detection of this species.
D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis*; D009174:Mycoplasma / Q000032:analysis* / Q000235:genetics; D009693:Nucleic Acid Hybridization; D013045:Species Specificity
D004269:DNA, Bacterial