GAWK, a novel human pituitary polypeptide: isolation, immunocytochemical localization and complete amino acid sequence.
During the course of reverse-phase high pressure liquid chromatography (RP-HPLC) purification of a postulated big ACTH (1) from human pituitary gland extracts, a highly purified peptide bearing no resemblance to any known polypeptide was isolated. The complete sequence of this 74 amino acid polypeptide, called GAWK, has been determined. Search on a computer data bank on the possible homology to any known protein or fragment, using a mutation data matrix, failed to reveal any homology greater than 30%. An antibody produced against a synthetic fragment allowed us to detect several immunoreactive forms. The antisera also enabled us to localize the polypeptide, by immunocytochemistry, in the anterior lobe of the pituitary gland.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D053378:Chromogranin B; D006651:Histocytochemistry; D006801:Humans; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000302:isolation & purification*; D010902:Pituitary Gland / Q000032:analysis*; D010903:Pituitary Gland, Anterior / Q000032:analysis; D014018:Tissue Distribution
D000596:Amino Acids; D053378:Chromogranin B; D009419:Nerve Tissue Proteins; C044449:GAWK