A replica method useful in the isolation of mutants of FM3A, a non-adherent cell line from mouse mammary carcinoma.
Existing replica methods designed for adherent somatic cells are not applicable to non-adherent cells of tumor origin. We have developed a new, efficient replica method for the negative selection of mutants of non-adherent mouse FM3A cells. Mutagenized cells are grown clonally on soft-agar plates then allowed to proliferate upwards through layered polyester disks, thus producing replicas with the same colony patterns. Isolation of mutants with increased requirements for myo-inositol by this method is described.
D000362:Agar / Q000502:physiology; D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002469:Cell Separation / Q000379:methods*; D003470:Culture Media; D008325:Mammary Neoplasms, Experimental / Q000145:classification / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains; D009154:Mutation
D003470:Culture Media; D000362:Agar