A new procedure to immobilize polyriboinosinic-polyribocytidylic acid (poly I:C) on the surface of polymethylmethacrylate and the use of poly I:C in clinical hemoperfusion.
A new procedure for covalent immobilization of polyriboinosinic-polyribocytidylic acid (poly I:C) on the surface of polymethylmethacrylate (PMMA) is described. Carboxyl groups are generated on the PMMA surface and react with primary amino groups of cytosine. These amino groups are made accessible by heating. The original polynucleotide structure is partly reformed on cooling. An extracorporeal chamber for in vivo whole blood perfusion made from PMMA has been previously described. Such chambers activated as described above by poly I:C have been used clinically. Considerable increase of cytotoxicity activity was observed and was long-lasting. No significant toxic side effects occurred and no refractoriness ensued during intermission between treatment periods. The same poly I:C chamber could be used several times in the same patient without loss of activity. Controls showed no leakage from the poly I:C mono-layer on the PMMA surfaces.
D000328:Adult; D002460:Cell Line; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D006464:Hemoperfusion / Q000379:methods*; D006801:Humans; D007370:Interferon Type I / Q000008:administration & dosage* / Q000627:therapeutic use; D007694:Killer Cells, Natural / Q000276:immunology; D008297:Male; D008545:Melanoma / Q000188:drug therapy* / Q000276:immunology; D020366:Methylmethacrylate; D008768:Methylmethacrylates; D008875:Middle Aged; D011070:Poly I-C / Q000008:administration & dosage* / Q000627:therapeutic use; D012509:Sarcoma
D007370:Interferon Type I; D008768:Methylmethacrylates; D020366:Methylmethacrylate; D011070:Poly I-C