Short bed-continuous development thin-layer chromatography of glycosphingolipids.
The technique of short bed--continuous development chromatography has been utilized to increase the separation of glycosphingolipids on high performance thin-layer chromatography plates. The theoretical goal of increasing separation of bands by decreasing solvent strength was achieved within a practical time span as a result of the high solvent velocities in the short bed tank. Examples are given for increased separation of short and long chain neutral glycolipids, acetylated neutral glycolipids, and gangliosides.
D000107:Acetylation; D000818:Animals; D002855:Chromatography, Thin Layer / Q000379:methods; D005260:Female; D005732:Gangliosides / Q000302:isolation & purification; D006017:Glycolipids / Q000302:isolation & purification; D006028:Glycosphingolipids / Q000302:isolation & purification*; D006801:Humans; D007118:Immunoassay; D007202:Indicators and Reagents; D007231:Infant, Newborn; D007940:Leukemia L5178; D008470:Meconium / Q000032:analysis; D051379:Mice
D005732:Gangliosides; D006017:Glycolipids; D006028:Glycosphingolipids; D007202:Indicators and Reagents