Expression of c-myc oncogene in human fibroblasts during in vitro senescence.
Expression of the c-myc oncogene was determined in pre-confluent early and late passage human (IMR-90) fibroblast by dot blot analysis of cellular mRNA. Significant decreases in c-myc levels were found in late passage when compared to levels found in early passage cells. Cells restimulated with serum after serum restriction also showed reduced levels of c-myc in late passage. Confluent cells expressed levels of c-myc similar to that of pre-confluent cells, when serum stimulation was the same in both cases. Southern blots of Eco R1 digested DNA showed 2 fragments of similar size hybridizing to c-myc sequences in both early and late passage cells.
D002470:Cell Survival; D005347:Fibroblasts / Q000502:physiology*; D005786:Gene Expression Regulation; D005796:Genes; D006801:Humans; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000235:genetics
D012333:RNA, Messenger