Effect of diabetes on the rhythm of [3H]thymidine incorporation in Con-A-stimulated mice splenocytes.
The chronogram of hyperglycaemia in alloxan-induced diabetic DBA/2 mice (living under conditions standardized for light-synchronized periodicity and fed ad libitum) presented an ultradian rhythm (during spring) different from the circadian blood glucose chronogram of normal control mice. Simultaneously, the [3H]thymidine ([3H]TdR) incorporation chronogram of diabetic mouse splenocytes, stimulated or unstimulated with Concanavalin-A (Con-A), was changed and unbalanced, compared to that of normal control mice. Previous experiments showed that the [3H]TdR incorporation chronogram of stimulated or non-stimulated splenocytes of normal DBA/2 mice presented seasonal variations. They were characterized generally by an ultradian rhythm. Yet, during spring, they exhibited a circadian rhythm because one phase was advanced and superimposed on the other, the latter being typically unvarying. It seems probable that the unbalanced rhythm of [3H]TdR incorporated in diabetic mouse splenocytes, stimulated or not, was responsible for a dysfunction of that population in diabetes.
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002940:Circadian Rhythm; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D010507:Periodicity; D012621:Seasons; D013154:Spleen / Q000473:pathology*; D013936:Thymidine / Q000378:metabolism
D001786:Blood Glucose; D013936:Thymidine