title: Contaminating fibrin in CPD-blood: solubility in plasma and distribution in blood components following separation.
abstract: In order to estimate the solubility of contaminating fibrin in CPD-blood, thrombin induced fibrin polymerzation in CPD-plasma was examined by light scattering and fibrinopeptide A (FPA) determinations. In addition, I125 fibrin monomer enriched CPD-blood was used to investigate fibrin monomer retention in blood bags and transfusion filters (170 microns) and fibrin distribution in blood components derived from CPD-blood. Initial fibrin polymerization in CPD-blood occurred after conversion of 15 per cent of the fibrinogen to fibrin, implying that substantial amounts of fibrin may be kept solubilized in CPD-blood bags. Only minor amounts of I125 fibrin monomers were retained in blood bags (2.4 per cent) and in transfusion filters (2.9 per cent) after sham transfusions. After separating I125-fibrin monomer enriched CPD-blood into its constituent components, the major part of fibrin (75.0 per cent) could be traced in the cryoprecipitate.
mesh_terms: D000225:Adenine; D000925:Anticoagulants; D001781:Blood Component Removal; D001793:Blood Preservation; D002951:Citrates; D005337:Fibrin / Q000302:isolation & purification*; D005374:Filtration; D005947:Glucose; D006801:Humans; D008027:Light; D010710:Phosphates; D011108:Polymers / Q000302:isolation & purification; D012542:Scattering, Radiation; D012995:Solubility
chemical_list: D000925:Anticoagulants; C034876:CPDA solutions; D002951:Citrates; D010710:Phosphates; D011108:Polymers; D005337:Fibrin; D005947:Glucose; D000225:Adenine