Measurement of prostaglandin E3 and other eicosanoids in biologic samples using high pressure liquid chromatography and radioimmunoassay.
A method to measure PGE3 in biologic samples is described. Complete resolution of PGE3 from PGE1 and PGE2 is achieved by reversephase high pressure liquid chromatography. Quantification is carried out by radioimmunoassay using an antibody directed against PGE2 that has high cross-reactivity with PGE3. Using this method, a marked increase in PGE3 production by mouse kidney tissue and in rat urine was demonstrated after supplemental feeding of omega-3 fatty acids. This method can also be applied to measurement of 6-keto-PGF1 alpha and TXB2 in the same samples.
D000527:Alprostadil / Q000031:analogs & derivatives*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003429:Cross Reactions; D004537:Eicosanoic Acids / Q000032:analysis*; D005260:Female; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism; D007672:Kidney Cortex / Q000032:analysis; D008297:Male; D051379:Mice; D009392:Nephrectomy; D011453:Prostaglandins; D011458:Prostaglandins E / Q000032:analysis* / Q000096:biosynthesis / Q000652:urine; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains; D013929:Thromboxane B2
D004537:Eicosanoic Acids; D011453:Prostaglandins; D011458:Prostaglandins E; D013929:Thromboxane B2; C033246:prostaglandin E3; D000527:Alprostadil