[Effect of cyclophosphane, chlorbutin and degranol on protein biosynthesis in the lymphocytes of donors and chronic lympholeukemia patients in vitro].
The alkylating agents in different concentrations are studied for their effect on protein biosynthesis and the relative content of leu-tRNA. The rate of elongation and termination of polypeptide translation in lymphocytes of donors and of patients with chronic lymphoid leukemia in vitro is studied. The therapeutic doses of cyclophosphamide are shown to inhibit the amino acid incorporation into acid insoluble pool of predominantly leukemic lymphocytes, while chlorbutin and degranol caused the greater suppression of the amino acid incorporation into healthy cells. Chlorbutin, cyclophosphamide and degranol in doses above therapeutic caused approximately 95, 48 and 34% inhibition of the protein synthesis, respectively. The mechanism of action on the translation is different. Only chlorbutin acted directly on the translation rate and also increased leu-tRNA content. Degranol decreased the leu-tRNA content. Cyclophosphamide produces no effect on these processes in vitro.
D000477:Alkylating Agents / Q000494:pharmacology*; D000596:Amino Acids / Q000097:blood; D001782:Blood Donors; D001798:Blood Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D002699:Chlorambucil / Q000494:pharmacology*; D003520:Cyclophosphamide / Q000494:pharmacology*; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007945:Leukemia, Lymphoid / Q000097:blood*; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008357:Mannomustine / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects
D000477:Alkylating Agents; D000596:Amino Acids; D001798:Blood Proteins; D002699:Chlorambucil; D003520:Cyclophosphamide; D008357:Mannomustine
Vliianie tsiklofosfana, khlorbutina i degranola na biosintez belka v limfotsitakh donorov i bol'nykh khronicheskim limfoleĭkozom in vitro.