Human marrow stromal cells in short-term semi-solid bone marrow culture in aplastic anaemia.
A short-term methylcellulose technique was used to study the proliferation of marrow-derived stromal, erythroid and myeloid colonies from normal controls and from patients with aplastic anaemia. There was a significant reduction in all colony types in patients with aplastic anaemia when compared with normal controls. In 4 patients who achieved remission following treatment with ATG + oxymetholone or with oxymetholone alone there was a return to normal range of stromal colonies and also of CFU-E. There was no change in the numbers of BFU-E's and only a slight increase in the numbers of CFU-C's.
D000293:Adolescent; D000328:Adult; D000741:Anemia, Aplastic / Q000473:pathology*; D001853:Bone Marrow / Q000473:pathology*; D002448:Cell Adhesion; D002454:Cell Differentiation; D002469:Cell Separation; D002478:Cells, Cultured; D002999:Clone Cells / Q000473:pathology; D003114:Colony-Forming Units Assay; D005260:Female; D005347:Fibroblasts / Q000473:pathology / Q000648:ultrastructure; D006412:Hematopoietic Stem Cells / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D013997:Time Factors