Measurement of lipase activity by a differential pH technique.
This is a new electrochemical method for determination of lipase activity in biological fluids, including serum, plasma, and duodenal juice. Advantages of turbidimetric methods--short reaction time, and small sample and reagent volumes--are combined with those of titrimetric methods: measurement of absolute activity (i.e., no standardization required), saturated substrate conditions, and direct measurement of reaction products. The proposed method is easy, inexpensive, and takes only 3 min. Precision is good: CV = 3.74% within day and 7.3% between days at the clinical-decision concentration, CV = 1.86% within day and 4.65% between days for above-normal lipase activities. The standard curve is linear up to 4500 U/L. Results (y) correlate well with those by turbidimetry (x): y = 0.9287x - 65.3 (r = 0.9719). Reference values are between 0 and 130 U/L.
D000328:Adult; D000368:Aged; D001647:Bile Acids and Salts; D002021:Buffers; D002412:Cations; D003089:Colipases; D004655:Emulsions; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008049:Lipase / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry; D009821:Oils; D000069463:Olive Oil; D010938:Plant Oils; D012016:Reference Values; D013379:Substrate Specificity; D014304:Triolein
D001647:Bile Acids and Salts; D002021:Buffers; D002412:Cations; D003089:Colipases; D004655:Emulsions; D009821:Oils; D000069463:Olive Oil; D010938:Plant Oils; D014304:Triolein; D008049:Lipase