title: Influence of different anesthetic agents on the release of cholecystokinin in dogs.
abstract: The purpose of this study was to investigate the effect of pentobarbital, halothane, and chloralose anesthesia on the endogenous release of cholecystokinin-33 (CCK-33) in dogs prepared with duodenal fistulas. Release of CCK-33 was induced by intraduodenal infusion of a medium-chain triglyceride (corn oil, 1 gm/kg/hr). Plasma CCK-33 concentrations were measured by means of a specific radioimmunoassay. Pentobarbital and chloralose were administered intravenously, and halothane was administered by a vaporizer (semiclosed technique), with O2 and N2O used as carriers. No incidence of hypotension was found with the use of these anesthetic agents. Basal concentrations of plasma CCK-33 were elevated, although not significantly, during pentobarbital or chloralose anesthesia. In conscious dogs (control study), peak plasma CCK-33 concentrations of 529 +/- 53 pg/ml were measured 30 minutes after intraduodenal infusion of fat. Under pentobarbital anesthesia, peak plasma CCK-33 concentrations of 452 +/- 264 pg/ml were found 80 minutes after infusion of fat. Under halothane anesthesia, fat-induced release of CCK-33 was abolished, whereas chloralose anesthesia did not influence fat-induced release of CCK-33. These findings may have implications for the design of future studies of gastrointestinal physiology. In CCK-33 studies that require anesthesia, chloralose appears to be an appropriate anesthetic agent.
mesh_terms: D000768:Anesthesia, General; D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D002698:Chloralose / Q000494:pharmacology; D002766:Cholecystokinin / Q000097:blood / Q000378:metabolism*; D003314:Corn Oil; D004285:Dogs; D005260:Female; D006221:Halothane / Q000494:pharmacology; D008297:Male; D009821:Oils; D010424:Pentobarbital / Q000494:pharmacology; D011863:Radioimmunoassay
chemical_list: D000777:Anesthetics; D009821:Oils; D002698:Chloralose; D003314:Corn Oil; D002766:Cholecystokinin; D010424:Pentobarbital; D006221:Halothane