[Effect of metal ions on the glucose isomerase activity of Streptomyces robeus S-606].
The intracellular glucose isomerase produced by Str. robeus S-606 refers to the group of isomerases activated most effectively by Mg2+. Besides, an activating effect of Fe3+, Co2+ and Mn2+ is observed. The optimal Mg2+ concentration for the D-glucose isomerization to D-fructose is 10(-2) M, and that of Co2+ is 100 times as low. Addition of Ca2+ (above 10% of the Mg2+ content) to the reaction mixture with the optimal Mg concentration inhibits the enzyme. At the same time Co2+ increases thermostability of glucose isomerase to a greater extent than Mg2+.
D019747:Aldose-Ketose Isomerases; D002238:Carbohydrate Epimerases / Q000378:metabolism*; D002412:Cations; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D008670:Metals / Q000494:pharmacology*; D013302:Streptomyces / Q000201:enzymology*
D002412:Cations; D008670:Metals; D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase
Vliianie ionov metallov na aktivnost' gliukozoizomerazy Streptomyces robeus S-606.