Cell-cycle differences of HL-60 leukemia cells fractionated by centrifugal elutriation.
HL-60 leukemia cells were fractionated into G1 and S/G2 populations using a rapid centrifugal elutriation technique, and studied for differences between the cell-cycle phases. The G1 fraction was found to contain smaller cells with a sedimentation velocity of 7 mm/h. The S/G2 fraction consisted of larger cells with a sedimentation velocity of 125 mm/h. The latter fraction was found to have a peak level of the enzyme (2'-5')An-binding protein, as compared to the G1 fraction, indicating a possible role for (2'-5')An-binding protein and its products in the growth regulation of these leukemic cells. In addition, cytofluorometric analysis of fractionated HL-60 cells indicates that elutriation is an effective fractionation method, rapidly yielding large numbers of cells for study, without the use of chemical treatments.
D002352:Carrier Proteins / Q000378:metabolism; D002453:Cell Cycle; D002460:Cell Line; D002469:Cell Separation / Q000379:methods; D002498:Centrifugation; D004722:Endoribonucleases; D006801:Humans; D007399:Interphase; D007938:Leukemia / Q000378:metabolism / Q000473:pathology*
D002352:Carrier Proteins; D004722:Endoribonucleases; C031956:2-5A-dependent ribonuclease