Red cell glycolytic intermediates and adenosine triphosphate in preterm infants on the first day of life.
Red cell glycolytic intermediates and ATP were evaluated in 47 appropriate for gestational age preterm infants on the 1st day of life who were divided into three groups on the basis of gestational age: 28-30, 31-33, and 34-36 wk. The results were compared to those previously obtained in term infants. The concentrations of glucose-6-phosphate, total triose phosphates, and ATP were significantly higher than in term infants but appeared to be appropriately elevated for the young mean age of the red cell population. The concentration of red cell 2,3-diphosphoglycerate (2,3-DPG) was significantly decreased when compared to term infants and was lowest at 28-30 wk gestation. The content of red cell 3-phosphoglycerate was increased in term infants and was inappropriately elevated for the age of the red cell population at 28-30 wk gestation. This pattern of glycolytic intermediates was suggestive of a young red cell population metabolizing at an increased glycolytic rate with increased flow through the phosphoglycerate kinase step rather than the 2,3-DPG bypass in "normal" preterm infants. Two preterm infants of 28-30 wk gestation with low red cell intracellular pH were also evaluated and had markedly decreased concentrations of red cell 2,3-DPG and ATP and all phosphorylated intermediates distal to the phosphofructokinase reaction, indicative of a cross-over at the phosphofructokinase step secondary to acidosis.(ABSTRACT TRUNCATED AT 250 WORDS)
D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate / Q000097:blood*; D004163:Diphosphoglyceric Acids / Q000097:blood; D004912:Erythrocytes / Q000201:enzymology*; D005636:Fructosephosphates / Q000097:blood; D005865:Gestational Age; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000097:blood; D005988:Glyceric Acids / Q000097:blood; D006019:Glycolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007231:Infant, Newborn; D007234:Infant, Premature; D010710:Phosphates / Q000097:blood; D010728:Phosphoenolpyruvate / Q000097:blood; D010735:Phosphoglycerate Kinase / Q000097:blood; D014306:Trioses / Q000097:blood
D004163:Diphosphoglyceric Acids; D005636:Fructosephosphates; D005958:Glucosephosphates; D005988:Glyceric Acids; D010710:Phosphates; D014306:Trioses; D019794:2,3-Diphosphoglycerate; C008885:2-phosphoglycerate; D019298:Glucose-6-Phosphate; C027618:fructose-6-phosphate; D010728:Phosphoenolpyruvate; C005156:3-phosphoglycerate; D000255:Adenosine Triphosphate; D010735:Phosphoglycerate Kinase