DNA-damaging properties and cytotoxicity in human fibroblasts of tetrachlorohydroquinone, a pentachlorophenol metabolite.
The DNA-damaging potential of pentachlorophenol (PCP) and its metabolite tetrachlorohydroquinone (TCH) was investigated. TCH was found to bind covalently to calf-thymus DNA and to cause single-strand breaks in PM2 DNA. No DNA-damaging effects were observed for PCP. Exposure of human fibroblasts to PCP and TCH showed that TCH is more toxic, when colony-forming ability after exposure to the agent is used as a measure of toxicity. In the evaluation of the mutagenic and carcinogenic potential of PCP the metabolite TCH should be taken into consideration.
D001711:Biotransformation; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002733:Chlorophenols / Q000633:toxicity*; D006801:Humans; D006873:Hydroquinones / Q000633:toxicity*; D009153:Mutagens; D010416:Pentachlorophenol / Q000031:analogs & derivatives / Q000633:toxicity*; D013329:Structure-Activity Relationship
D002733:Chlorophenols; D006873:Hydroquinones; D009153:Mutagens; C044578:2,3,5,6-tetrachlorohydroquinone; D010416:Pentachlorophenol