ADP-ribosylation induced changes in the conformation of the chromatin of the brain of developing rats.
Conformational changes in the chromatin of the cerebral hemisphere of 3-, 14- and 30-day old developing rats were studied before and after its ADP-ribosylation using DNase I and micrococcal nuclease (MNase). The rate and extent of digestion of chromatin by DNase I are the highest at 3-day and decline progressively thereafter. The rate and extent of digestion by MNase do not change during development. ADP-ribosylation of chromosomal proteins was carried out by incubating nuclei with NAD+ for 30 min and was followed by endonuclease digestion. Both the rate and extent of digestion by DNase I and MNase were enhanced after ADP-ribosylation which was the maximum for 3-day rats.
D000246:Adenosine Diphosphate Ribose / Q000494:pharmacology*; D000375:Aging; D000818:Animals; D001921:Brain / Q000254:growth & development*; D002467:Cell Nucleus / Q000648:ultrastructure; D002843:Chromatin / Q000187:drug effects / Q000648:ultrastructure*; D004247:DNA / Q000302:isolation & purification; D003850:Deoxyribonuclease I / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008836:Micrococcal Nuclease / Q000378:metabolism; D009702:Nucleoside Diphosphate Sugars / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D002843:Chromatin; D009702:Nucleoside Diphosphate Sugars; D000246:Adenosine Diphosphate Ribose; D004247:DNA; D003850:Deoxyribonuclease I; D008836:Micrococcal Nuclease