Conversion of dense lysosomes into light lysosomes during hepatocytic autophagy.
Incubation of isolated rat hepatocytes under conditions which support maximal autophagy (amino acid-free medium) caused a marked alteration in the density distribution of lysosomes in continuous metrizamide gradients (mean peak density reduced from 1.14 to 1.09 g/ml). The autophagic sequestration inhibitor 3-methyladenine (3MA) partially prevented the density shift, presumably by stopping the formation of light autophagosomes which otherwise fuse with dense lysosomes and thereby alter the lysosomal density.
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001343:Autophagy / Q000187:drug effects; D002499:Centrifugation, Density Gradient; D008099:Liver / Q000502:physiology / Q000648:ultrastructure*; D008247:Lysosomes / Q000378:metabolism / Q000502:physiology*; D008297:Male; D008928:Mitochondria; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013395:Sucrose / Q000378:metabolism
C025946:3-methyladenine; D013395:Sucrose; D000225:Adenine