Quantifying adductive modification of hemoglobin from mice exposed to benzo[a]pyrene.
The present work describes a method for the detection of minute amounts of benzo[a]pyrene, as the diolepoxide metabolite, bound covalently to the hemoglobin of erythrocytes isolated from mice previously exposed to the carcinogen. The technique consists of the acid-induced removal of the pyrenyl moiety from the hemoglobin as the strongly fluorescent free tetrols and their isolation by bonded-phase extraction methods and subsequent quantitation by fluorescence/HPLC. With this procedure as little as 5 pg of tetrol can be detected. The assay was used to determine the amount of benzo[a]pyrene-hemoglobin adduct formation in mice bearing a carcinogen-induced fibrosarcoma.
D000818:Animals; D001564:Benzo(a)pyrene / Q000097:blood* / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid; D005354:Fibrosarcoma / Q000097:blood / Q000139:chemically induced; D006454:Hemoglobins / Q000378:metabolism*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D013050:Spectrometry, Fluorescence
D006454:Hemoglobins; D001564:Benzo(a)pyrene