Detection of delta-aminolevulinic acid, porphobilinogen and porphyrins related to heme biosynthesis by high-performance liquid chromatography.
Two new high-performance liquid chromatographic methods are described for the quantitative determination of porphyrins and their precursors. In our method, sub-nanomole quantities of porphyrins, delta-aminolevulinic acid and porphobilinogen derivatized with ophthalaldehyde were injected onto a C18 reversed-phase column and eluted with 0.1 M monobasic sodium phosphate-methanol-tetrahydrofuran (4:6:3) and detected with a spectrofluorometer. A second reversed-phase system using methanol-tetrahydrofuran-22 mM acetate buffer (15:6:11) was also developed.
D000622:Aminolevulinic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006418:Heme / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007982:Levulinic Acids / Q000032:analysis*; D011162:Porphobilinogen / Q000032:analysis*; D011166:Porphyrins / Q000032:analysis*; D009764:o-Phthalaldehyde
D007202:Indicators and Reagents; D007982:Levulinic Acids; D011166:Porphyrins; D006418:Heme; D009764:o-Phthalaldehyde; D011162:Porphobilinogen; D000622:Aminolevulinic Acid