Microplate solid-phase radioimmunoassay for rat prolactin.
A rat prolactin solid-phase radioimmunoassay has been developed that uses 96-well microtiter plates with removable wells to which the antibody is firmly adsorbed, resulting in a solid-phase antibody. Antigen as either reference or unknown competes with radioactivity labeled antigen for binding sites on the solid-phase antibody. After immunoreaction, free antigen is removed by washing the wells with phosphosaline solution. The solid-phase antibody-antigen complex is counted for quantitation with data reduction methods currently used in routine radioimmunoassay procedures. This microplate solid-phase radioimmunoassay has several advantages over conventional methods without sacrificing specificity, sensitivity, or accuracy. This method is rapid, compact, economical, easily automated, and could be readily established in other laboratories.
D000818:Animals; D005069:Evaluation Studies as Topic; D008832:Microchemistry; D011388:Prolactin / Q000097:blood*; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D012015:Reference Standards
D011388:Prolactin