Radioenzymatic assay for trimethoprim in very small serum samples.
A modification of the methotrexate radioassay kit (supplied by New England Enzyme Center) enabled determination of trimethoprim levels in 5-microliter serum samples. An excellent correlation between this assay and high-pressure liquid chromatography assay was found. These preliminary results suggest that with this method rapid determination of trimethoprim levels in very small samples (5 to 10 microliters) can be achieved.
D006801:Humans; D007457:Iodine Radioisotopes; D008727:Methotrexate / Q000097:blood; D011933:Reagent Kits, Diagnostic; D013762:Tetrahydrofolate Dehydrogenase; D014295:Trimethoprim / Q000097:blood*
D007457:Iodine Radioisotopes; D011933:Reagent Kits, Diagnostic; D014295:Trimethoprim; D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate