The acute effects of ethanol on protein synthesis in isolated rat liver cells are pH-dependent.
Acute administration of ethanol to isolated rat liver cells induced a pH-dependent inhibition of protein synthesis. The effect of the alcohol was highest at pH 7.0 and nil at pH 7.8. 4-methyl-pyrazole partially reversed the action of ethanol only below pH 7.4. Time-course experiments suggested that ethanol could act preventing the initiation of new polypeptide chains stimulated by D-glucose, and that this effect is abolished at pH 7.8.
D000818:Animals; D000431:Ethanol / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium; D014633:Valine / Q000378:metabolism
D014316:Tritium; D000431:Ethanol; D014633:Valine