title: Simple method for preparing the cellular intermediate EAC14, and its use for estimation of the second component of complement.
abstract: Sensitized erythrocytes carrying the first and fourth components of complement (EAC14) were prepared by incubating optimally sensitized sheep erythrocytes with normal serum appropriately diluted in Mg2+-free diethylbarbiturate buffer containing Ca2+. EAC14 cells so prepared were found to be suitable for use in estimating the second component of complement (C2) in human serum, and the method is described here.
mesh_terms: D000818:Animals; D002021:Buffers; D003167:Complement Activation; D003175:Complement C2 / Q000032:analysis* / Q000172:deficiency; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D008297:Male; D012756:Sheep
chemical_list: D002021:Buffers; D003175:Complement C2