DNA-mediated transfer of actinomycin D resistance into cultured mouse cells.
The transfer of high molecular weight (HMW) DNA isolated from Syrian hamster actinomycin D-resistant cells (C12TSV5/R) to sensitive A9 mouse cells, resulted in the growth in selective medium of transferent clones A9 ActR C11, C12 and C13). The resistant mouse cells have presumably acquired a gene(s) conferring actinomycin D resistance and express a 140-150 K surface glycoprotein, apparently involved in the drug resistance.
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D006224:Cricetinae; D003609:Dactinomycin / Q000494:pharmacology*; D004351:Drug Resistance; D005796:Genes; D006023:Glycoproteins / Q000096:biosynthesis / Q000235:genetics; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008970:Molecular Weight; D014162:Transfection
D006023:Glycoproteins; D008565:Membrane Proteins; D003609:Dactinomycin