An evaluation of commercial discs for the determination of the growth factor requirements of avian haemophili.
The ability of 2 brands of growth factor discs (Oxoid and Mast) to identify correctly the growth factor requirements of 41 isolates of Haemophilus paragallinarum and 17 isolates of H. avium was evaluated. The percentage of isolates correctly identified as requiring V factor varied with both the brand of the disc and the medium used. On basic nutrient media both brands gave low percentages of correct results: on Isosensitest Agar (Oxoid) the Oxoid discs gave 26% and the Mast discs 24% while on Heart Infusion Agar (Gibco) the Oxoid discs gave 54% and the Mast discs 28%. However, on a more complex medium (TM/S) the percentage of correct results was considerably higher with the Oxoid discs 100% and the Mast discs 52% accurate.
D000818:Animals; D001717:Birds / Q000382:microbiology*; D003470:Culture Media; D005069:Evaluation Studies as Topic; D006190:Haemophilus / Q000187:drug effects / Q000254:growth & development*; D006427:Hemin / Q000494:pharmacology; D009243:NAD / Q000494:pharmacology
D003470:Culture Media; D009243:NAD; D006427:Hemin