title: Physiological plasma concentrations of cholecystokinin stimulate pancreatic enzyme secretion and gallbladder contraction in man.
abstract: The present study was undertaken to determine whether infusion of cholecystokinin (CCK) to plasma concentrations comparable to those found after a meal stimulates pancreatic enzyme secretion and gallbladder contraction. Plasma CCK concentrations were measured by radioimmunoassay using antibody T204, which binds to all carboxyl-terminal CCK-peptides containing the sulfated tyrosine region. Ingestion of a standardized test meal in 7 normal subjects induced significant increases in plasma CCK from 2.0 +/- 0.2 pmol/l to levels between 4.6 +/- 0.6 and 7.3 +/- 1.0 pmol/l (p less than 0.05-p less than 0.0005). Infusion of 2.5 pmol/kg X h CCK 33 resulted in significant increases in plasma CCK from 2.0 +/- 0.2 to 3.9 +/- 0.3 pmol/l (p less than 0.0005). This infusion of CCK induced significant increases in trypsin secretion from 0.5 +/- 0.1 to 1.4 +/- 0.2 KU/15 min (p less than 0.005) and in bilirubin output from 1.6 +/- 0.7 to 30.3 +/- 8.0 mumol/15 min (p less than 0.05). It is concluded that physiological plasma concentrations of CCK stimulate pancreatic enzyme secretion and gallbladder contraction in man.
mesh_terms: D000328:Adult; D001663:Bilirubin / Q000378:metabolism; D002766:Cholecystokinin / Q000097:blood*; D004386:Duodenum / Q000378:metabolism; D005260:Female; D005502:Food; D005704:Gallbladder / Q000502:physiology*; D006801:Humans; D008297:Male; D009119:Muscle Contraction; D010179:Pancreas / Q000201:enzymology*; D011863:Radioimmunoassay; D012016:Reference Values; D014357:Trypsin / Q000378:metabolism
chemical_list: D002766:Cholecystokinin; D014357:Trypsin; D001663:Bilirubin