title: Replication and polypeptide synthesis of Mill Door/79, an orbivirus isolated from ticks from a seabird colony in Scotland.
abstract: The replication and polypeptide synthesis of orbivirus isolate Mill Door/79, a member of the Kemerovo serogroup, were studied. In Vero cells cell-associated virus exceeded cell-free virus by about 2 log10 PFU/ml. Attempts to purify the virus resulted in the demonstration of five polypeptides. Thirteen virus-induced polypeptides and 10 segments of double-stranded RNA were identified in infected cells. Partial proteolysis demonstrated homology between some polypeptides.
mesh_terms: D000818:Animals; D001717:Birds / Q000469:parasitology; D002460:Cell Line; D002522:Chlorocebus aethiops; D005814:Genes, Viral; D012330:RNA, Double-Stranded / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D012087:Reoviridae / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D012606:Scotland; D013987:Ticks / Q000382:microbiology; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis*; D014779:Virus Replication
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins