title: Pharmacological study on the effects of some ocular hypotensive drugs on aqueous humor formation in the arterially perfused enucleated rabbit eye.
abstract: The effects of pilocarpine, isoproterenol, epinephrine, timolol, acetazolamide, ouabain, and dinitrophenol on aqueous humor production were investigated in enucleated rabbit eye. The metabolic inhibitors ouabain and dinitrophenol at a concentration of 10(-5) M produced a 50% inhibition in aqueous humor formation. Acetazolamide at the same concentration was without effect, but at 10(-4) M concentration lowered aqueous production by 40%. The autonomic drugs epinephrine and isoproteranol at a concentration of 10 micrograms/ml medium reduced aqueous humor formation by 64 and 52% respectively. Timolol, even at a concentration of 0.05 microgram/ml, suppressed aqueous humor formation by approximately 30%. Pilocarpine on the other hand was ineffective. Except for pilocarpine, these responses are similar to those reported to occur in the cat both in vivo and in vitro.
mesh_terms: D000086:Acetazolamide / Q000494:pharmacology*; D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism*; D001158:Arteries; D001337:Autonomic Agents / Q000494:pharmacology*; D004140:Dinitrophenols / Q000494:pharmacology*; D004837:Epinephrine / Q000494:pharmacology; D066298:In Vitro Techniques; D007429:Intraocular Pressure / Q000187:drug effects*; D007545:Isoproterenol / Q000494:pharmacology; D010042:Ouabain / Q000494:pharmacology*; D010477:Perfusion; D010862:Pilocarpine / Q000494:pharmacology; D011817:Rabbits; D013997:Time Factors; D013999:Timolol / Q000494:pharmacology
chemical_list: D001337:Autonomic Agents; D004140:Dinitrophenols; D010862:Pilocarpine; D010042:Ouabain; D013999:Timolol; D007545:Isoproterenol; D000086:Acetazolamide; D004837:Epinephrine