Amino acid sequence of human liver cathepsin B.
The complete amino acid sequence of cathepsin B (EC 3.4.22.1) from human liver was determined. The 252-residue sequence was obtained by automated solid-phase Edman degradation of the light and heavy chain resulting from limited proteolysis of the single-chain enzyme and of fragments produced by cyanogen bromide and enzymatic cleavage of the heavy chain. Human liver cathepsin B has 83.7% identical residues with the corresponding enzyme from rat liver. Comparison of both mammalian cathepsin B sequences with the sequence of papain provides further evidence that lysosomal and plant cysteine proteinases have evolved from a common ancestor and share a similar catalytic mechanism.
D000595:Amino Acid Sequence; D002401:Cathepsin B; D002403:Cathepsins / Q000032:analysis*; D006801:Humans; D008099:Liver / Q000201:enzymology*
D002403:Cathepsins; D002401:Cathepsin B