title: Blockage of tropoelastin secretion by monensin represses tropoelastin synthesis at a pretranslational level in rat smooth muscle cells.
abstract: The blockage of protein secretion in the R22 cultured rat aortic smooth muscle cell strain with monensin repressed tropoelastin gene expression at the mRNA level by ca. 50-fold as measured by biosynthetic pulse-labeling, in vitro translation, and hybridization with a tropoelastin genomic DNA probe. These results suggest that tropoelastin gene expression is autoregulated, and they represent the first reported effect of monensin on gene expression.
mesh_terms: D000818:Animals; D004549:Elastin / Q000031:analogs & derivatives*; D005663:Furans / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D008985:Monensin / Q000494:pharmacology*; D009130:Muscle, Smooth / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012636:Secretory Rate / Q000187:drug effects; D014333:Tropoelastin / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
chemical_list: D005663:Furans; D012333:RNA, Messenger; D014333:Tropoelastin; D004549:Elastin; D008985:Monensin