Separation and detection of closely related endorphins by liquid chromatography-electrochemistry.
The specific aim of this study was to investigate the selectivity of liquid chromatography--electrochemistry for minor variations in the structure of small endorphins. Using isocratic mobile phases, chromatographic conditions were established for the separation of a series of closely related endorphins. Hydrodynamic voltammetry showed that each peptide exhibited a characteristic oxidative behavior that was also reflected in peak current ratios. Changes in a small moiety altered both the chromatographic behavior and electroactivity of these neuropeptides.
D002853:Chromatography, Liquid; D004563:Electrochemistry; D004566:Electrodes; D004723:Endorphins / Q000032:analysis* / Q000302:isolation & purification; D012834:Silver; D018030:Silver Compounds
D004723:Endorphins; D018030:Silver Compounds; D012834:Silver; C037548:silver chloride