Measurement of glycosylated whole-blood protein for assessing glucose control in diabetes: collection and storage of capillary blood on filter paper.
We present data on the use of filter-paper blood collection for measurement of glycosylated whole-blood proteins (gWB) (hemoglobin and plasma proteins). A capillary blood sample, obtained by fingerprick, is spotted directly onto filter paper (Schleicher & Schuell 903). The blood spot is washed briefly with alcohol (ethanol or isopropanol) to remove free glucose and dried before shipment to the laboratory. In the laboratory, the blood is eluted from the paper and analyzed for gWB by a colorimetric method. The gWB is primarily a measure of glycosylated hemoglobin (gHb) with a small contribution from glycosylated plasma protein. Concentrations of gWB and gHb are highly correlated (r = 0.91). The filter-paper method offers advantages over currently available methods for quantifying gHb and may be particularly useful in screening for diabetes and for assessing glycemic control in patients from remote areas.
D001798:Blood Proteins / Q000032:analysis*; D001800:Blood Specimen Collection / Q000379:methods*; D002196:Capillaries; D002851:Chromatography, High Pressure Liquid; D003920:Diabetes Mellitus / Q000097:blood*; D004912:Erythrocytes / Q000032:analysis; D005374:Filtration; D006442:Glycated Hemoglobin / Q000032:analysis; D006023:Glycoproteins; D006801:Humans; D010209:Paper; D013048:Specimen Handling; D014680:Veins; D000093422:Glycated Serum Proteins
D001798:Blood Proteins; D006442:Glycated Hemoglobin; D006023:Glycoproteins; D000093422:Glycated Serum Proteins