title: Maturation of adult rat peritoneal and mesenteric mast cells. A morphological and histofluorescence study.
abstract: Repopulation and maturation of rat mesenteric and peritoneal mast cells were studied after mast cell depletion by intraperitoneal injection of distilled water. Immature mast cells were first identified in the mesentery and peritoneal fluid 5 and 6 days, respectively, after water injection. The most immature mast cells that could be identified contained a few orthochromatic granules. Upon maturation, the granules became metachromatic and increased in size and number. Heparin, revealed by toluidine blue staining and berberine sulfate fluorescence, appeared simultaneously with orthophthaldialdehyde (OPT)-induced histamine fluorescence. Paraformaldehyde-induced serotonin fluorescence appeared somewhat later. Repopulation of mesentery and peritoneal fluid by mast cells seemed to be independent of each other and to occur from undifferentiated precursor cells.
mesh_terms: D000818:Animals; D001599:Berberine; D002454:Cell Differentiation; D005260:Female; D006632:Histamine / Q000032:analysis; D006651:Histocytochemistry; D008297:Male; D008407:Mast Cells / Q000032:analysis / Q000166:cytology*; D008643:Mesentery / Q000166:cytology*; D008856:Microscopy, Fluorescence; D009000:Monocytes / Q000032:analysis / Q000166:cytology; D010529:Peritoneal Cavity / Q000166:cytology*; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000032:analysis
chemical_list: D001599:Berberine; D012701:Serotonin; D006632:Histamine