Resonance Raman spectroscopic studies of adriamycin and copper(II)-adriamycin and copper(II)-adriamycin-DNA complexes.
Characteristic resonance Raman spectra are observed on ionization of the phenolic groups in adriamycin. On the basis of these results, vibrational assignments for the Raman bands of adriamycin are reported. Distinct Raman spectra are observed for Cu(II)-adriamycin complexes at pH approximately 5 and pH approximately 13. The data indicate that at lower pH a bis complex of Cu(II) is formed, which transforms to a polymeric Cu(II) chelate at higher pH. Upon interaction of the metal-drug complex with calf thymus DNA at pH approximately 5, a ternary complex is formed in which the Cu(II)-complexed adriamycin is intercalated into DNA.
D000818:Animals; D002417:Cattle; D003300:Copper; D004247:DNA; D004317:Doxorubicin; D006863:Hydrogen-Ion Concentration; D013059:Spectrum Analysis, Raman; D013329:Structure-Activity Relationship; D013950:Thymus Gland
D003300:Copper; D004317:Doxorubicin; D004247:DNA