Acylated proteins in Acholeplasma laidlawii.
The covalent modification of membrane proteins by long-chain fatty acids was determined in two strains of Acholeplasma laidlawii by one-dimensional gel electrophoresis of radiolabeled membranes. Of the more than 50 membrane polypeptides detected, approximately 30 were labeled with [3H]palmitate, whereas covalent binding of [3H]oleate to membrane proteins could not be demonstrated. We suggest that in these wall-less bacteria, membrane protein acylation with saturated fatty acids may serve to ensure the structural integrity of the membrane.
D000128:Acholeplasma laidlawii / Q000032:analysis*; D000215:Acylation; D001426:Bacterial Proteins / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis*; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism
D001426:Bacterial Proteins; D008565:Membrane Proteins; D010169:Palmitic Acids; D019308:Palmitic Acid