New assay for the O-alkyl cleavage enzyme with alkyl-lysophospholipids as substrates.
The O-alkyl cleavage enzyme is important for the metabolism of cytotoxic alkyl-lysophospholipids. We have developed a simple new method for the determination of the enzyme activity which is based on the formation of water-soluble phosphate during the enzyme reaction. This is the first assay which avoids the use of radiolabeled substrates.
D000818:Animals; D006867:Hydrolases / Q000378:metabolism*; D008246:Lysophospholipids; D008862:Microsomes, Liver / Q000201:enzymology; D010739:Phospholipase D / Q000378:metabolism*; D010740:Phospholipases / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D011621:Pteridines / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D010738:Type C Phospholipases / Q000378:metabolism*
D008246:Lysophospholipids; D010743:Phospholipids; D011621:Pteridines; C005063:2-amino-4-hydroxy-6,7-dimethyl-5,6,7,8-tetrahydropteridine; D006867:Hydrolases; D010740:Phospholipases; D010738:Type C Phospholipases; D010739:Phospholipase D; C051319:O-alkyl cleavage enzyme