Development of a radioimmunoassay for human deoxyribonuclease I.
A reliable radioimmunoassay (RIA) for human deoxyribonuclease I (DNase I) is described. Using delayed addition of tracer antigen, the method is sensitive (9.5 reproducible and specific. A good parallel relationship was observed between the standard curve and dilution curves for human urine and human pancreatic juice. G-actin, a naturally occurring DNase I inhibitor, caused no change in the immunoreactivity of DNase I. In healthy individuals, aged 11-90 yr, the mean serum DNase I was 18.4 ng/ml (SD 6.7 ng/ml). Increased serum DNase I occurred in patients with acute pancreatitis, renal failure, and in about one-third of patients with various malignant tumors.
D000199:Actins / Q000494:pharmacology; D000293:Adolescent; D000328:Adult; D000368:Aged; D000941:Antigens / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003850:Deoxyribonuclease I / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007223:Infant; D008875:Middle Aged; D009369:Neoplasms / Q000201:enzymology; D010189:Pancreatic Juice / Q000201:enzymology; D011863:Radioimmunoassay / Q000379:methods*
D000199:Actins; D000941:Antigens; D003850:Deoxyribonuclease I