title: Pyruvate kinase of sea bass liver: interrelationship in regulating properties.
abstract: Pyruvate kinase of sea bass liver show a joint modulation of both pH and temperature for PEP substrate. The effect of F-1,6-P2, alanine and ATP is not fundamentally affected by a variation in pH. The kinetic constants in the presence of ATP are not affected, but the intensity of its inhibitor effect varies with temperature. A study of different buffers: Tris-HC1, Tris-maleic and phosphate, on this enzymatic activity with or without effectors has been made.
mesh_terms: D000409:Alanine / Q000494:pharmacology; D000818:Animals; D005399:Fishes; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D011770:Pyruvate Kinase / Q000378:metabolism*; D013816:Thermodynamics
chemical_list: D011770:Pyruvate Kinase; D000409:Alanine