title: Novel insertion mutation in Caenorhabditis elegans.
abstract: The mutation e1662 is an allele of the Caenorhabditis elegans unc-54 gene induced with the difunctional alkylating agent 1,2,7,8-diepoxyoctane. unc-54 encodes the major myosin heavy chain isozyme of body wall muscle cells. Filter-transfer hybridization and DNA sequence analysis show that e1662 is an insertion of 288 base pairs of DNA within unc-54. The inserted DNA is identical to a 288-base pair region of unc-54 located ca. 600 base pairs from the insertion site. Thus, e1662 is a displaced duplication. A 14-base pair sequence located at one end of the duplicated segment is found adjacent to the site of insertion. These homologous sequences are juxtaposed head-to-tail by the insertion event. e1662 thus contains a tandem direct repeat extending across one of its junctions.
mesh_terms: D000477:Alkylating Agents / Q000633:toxicity*; D000818:Animals; D001483:Base Sequence; D002107:Caenorhabditis / Q000235:genetics*; D004852:Epoxy Compounds / Q000633:toxicity*; D004988:Ethers, Cyclic / Q000633:toxicity*; D005796:Genes; D009154:Mutation / Q000187:drug effects*; D009218:Myosins / Q000235:genetics
chemical_list: D000477:Alkylating Agents; D004852:Epoxy Compounds; D004988:Ethers, Cyclic; C012896:1,2,7,8-diepoxyoctane; D009218:Myosins