title: K, Ca, Mg, and Zn in platelets, as determined by atomic absorption spectrometry with use of a sealed decomposition bomb and discrete nebulization.
abstract: In this new method for precise and accurate determination of K, Ca, Mg, and Zn in platelets, small amounts of platelets, prepared by differential centrifugation and cell washing, are decomposed in a homemade mini-vessel, a sealed Teflon bomb. The metal ions in the decomposed sample are measured by atomic absorption spectrometry with discrete nebulization. Overall, CVs ranged from 0.8 to 4.8%. We investigated sex-related differences (none were found) and intra-individual variations.
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000032:analysis*; D002118:Calcium / Q000097:blood; D002469:Cell Separation / Q000379:methods; D005260:Female; D006801:Humans; D008274:Magnesium / Q000097:blood; D008297:Male; D008670:Metals / Q000097:blood*; D011188:Potassium / Q000097:blood; D012737:Sex Factors; D013054:Spectrophotometry, Atomic / Q000295:instrumentation; D013997:Time Factors; D015032:Zinc / Q000097:blood
chemical_list: D008670:Metals; D008274:Magnesium; D015032:Zinc; D011188:Potassium; D002118:Calcium