Isolation of bovine eosinophils by density gradient centrifugation.
A method for the isolation of an enriched population (greater than 60%) of bovine eosinophils was developed, using density gradient centrifugation. Eosinophils were isolated from peripheral blood by centrifugation on a density gradient medium of sp gr 1.092. Viability was greater than or equal to 95%, and the isolated eosinophils were found to be functional in chemokinetic and antibody-dependent cellular-cytotoxicity assays. This method represents a simple, rapid method for obtaining bovine eosinophils that can be used in a variety of assays of eosinophil function.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D002417:Cattle / Q000097:blood*; D002499:Centrifugation, Density Gradient / Q000379:methods; D002634:Chemotaxis, Leukocyte; D004804:Eosinophils / Q000166:cytology* / Q000276:immunology / Q000502:physiology; D015054:Zymosan
D015054:Zymosan