[Effect of the chromatographic purification method on the isolation of prostaglandins E and F from a natural lipid mixture and their stability].
Separation of PGE2 and PGF2 alpha and their ethyl ethers on silica gel from a natural lipid mixture permits the attainment of the 97-100% purity of the substances. However, the losses for PGE2 amount to 20-25, those for PGF2 alpha to 35-40%. Thus chromatography on silica gel can be used only for analytical purposes. As regards the preparative purposes the best method of PG isolation from a natural lipid mixture is gel filtration with a preliminary separation of phospholipids from the mixture over the oleophilic sulfacationite KO-I. After separation on sephadex the preparations can be stored for up to 4-6 months at 5-8 degrees C.
D000818:Animals; D002417:Cattle; D002849:Chromatography, Gas / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D004355:Drug Stability; D008055:Lipids / Q000302:isolation & purification*; D008297:Male; D010743:Phospholipids / Q000302:isolation & purification; D011458:Prostaglandins E / Q000302:isolation & purification*; D011460:Prostaglandins F / Q000302:isolation & purification*; D012756:Sheep
D008055:Lipids; D010743:Phospholipids; D011458:Prostaglandins E; D011460:Prostaglandins F
Vliianie sposoba khromatograficheskoĭ ochistki na vydelenie prostaglandinov E i F iz prirodnoĭ smesi lipidov i ikh ustoĭchivost'.