title: Transport and steady-state concentration of plasma proteins in the vitreous humor of the chicken embryo: implications for the mechanism of eye growth during early development.
abstract: The nature and origin of the proteins of the vitreous humor were examined in chickens during embryonic and early posthatching stages. The major proteins of the vitreous humor were similar in electrophoretic mobility to plasma proteins at all ages examined. Earlier studies from our laboratory and experiments described below showed that plasma proteins continuously entered and left the eye throughout its development. From these data it was concluded that the majority of vitreous-humor proteins were derived from the blood. The protein concentration of the vitreous humor was 13% of that of the plasma from embryonic Days 6 through 15 (E6 through E15). After E15, the relative protein concentration in the vitreous humor declined with respect to the plasma and reached 4% of the plasma protein concentration at hatching. Several possibilities were considered to account for how proteins can rapidly enter and leave the eye, yet maintain a steady-state concentration in the vitreous humor that is less than one-seventh of that in the blood.
mesh_terms: D000818:Animals; D001692:Biological Transport; D001797:Blood Protein Electrophoresis; D001798:Blood Proteins / Q000378:metabolism*; D002642:Chick Embryo; D002645:Chickens; D005123:Eye / Q000196:embryology* / Q000254:growth & development; D006207:Half-Life; D014822:Vitreous Body / Q000032:analysis*
chemical_list: D001798:Blood Proteins