[Morphogenetic characteristics of the aggregates formed in epithelial and mesenchymal recombinations of the lungs from intact and urethane-exposed mouse embryos].
A study was made of the morphogenesis of organotypic aggregates obtained by epithelial mesenchymal recombinations from the lungs of embryonic mice, intact and treated with urethane. Normal growth and differentiation of organotypic structures were observed in long-term cultures of aggregates obtained by recombinations of the lung epithelium (E) and mesenchyma (M) from intact (i) embryonic mice (EiMi). Hyperplasia and squamous-cell metaplasia (with or without keratinization) of the epithelium were found in aggregates obtained from E and M of the treated mouse embryos (EtMt) and in aggregates obtained by recombinations of lung E and M from intact and treated embryos (EtMi, EiMt). The data obtained suggest that the alterations in epithelial mesenchymal interactions are of great significance for transplacental lung blastomogenesis and that the mesenchymal lung cells play an important part in mediation of the transplacental carcinogenous effects on epithelial target cells via subsequent epithelial mesenchymal tissue interactions.
D000818:Animals; D046508:Culture Techniques; D004848:Epithelium / Q000187:drug effects / Q000196:embryology; D005260:Female; D006965:Hyperplasia / Q000139:chemically induced / Q000196:embryology; D008168:Lung / Q000187:drug effects / Q000196:embryology*; D008175:Lung Neoplasms / Q000139:chemically induced / Q000196:embryology; D008648:Mesoderm / Q000166:cytology* / Q000187:drug effects; D008679:Metaplasia / Q000139:chemically induced / Q000196:embryology; D051379:Mice; D008805:Mice, Inbred A; D009024:Morphogenesis / Q000187:drug effects; D011247:Pregnancy; D014520:Urethane / Q000494:pharmacology*
D014520:Urethane
Osobennosti morfogeneza agregatov, obrazuiushchikhsia pri rekombinatsiiakh épiteliia i mezenkhimy legkikh intaktnykh i podvergavshikhsia vozdeĭstviiu uretana émbrionov mysheĭ.