In situ nick translation of human and mouse chromosomes detected with a biotinylated nucleotide.
In situ nick translation of human and mouse chromosome preparations has provided further evidence that DNase I sensitivity correlates with transcriptionally active chromatin. Chromosomes from differentiating cells and particular chromosome regions, known to differ in transcriptional activity, generally reveal predicted differences in relative DNase I sensitivity. The application of a biotinylated nucleotide in these studies provides a new level of cytological resolution and offers the potential for further refinement.
D000818:Animals; D001345:Autoradiography; D001710:Biotin / Q000032:analysis / Q000378:metabolism*; D001755:Blastocyst / Q000378:metabolism; D002842:Chromatids / Q000378:metabolism; D002871:Chromosome Banding; D002875:Chromosomes / Q000378:metabolism*; D002877:Chromosomes, Human / Q000378:metabolism*; D004256:DNA Polymerase I / Q000494:pharmacology; D003850:Deoxyribonuclease I / Q000494:pharmacology; D003856:Deoxyuracil Nucleotides / Q000378:metabolism*; D006801:Humans; D008677:Metaphase; D051379:Mice; D008938:Mitosis; D009697:Nucleolus Organizer Region / Q000378:metabolism; D014176:Protein Biosynthesis
D003856:Deoxyuracil Nucleotides; D001710:Biotin; D004256:DNA Polymerase I; D003850:Deoxyribonuclease I