Comparison of Class II HLA antigen expression in normal and carcinomatous human breast cells.
Class II HLA antigen expression in breast carcinoma and normal breast gland cells was compared using a method more accurate than immunofluorescence. This new method involves labeling membrane proteins with 131I and the anti-Class II HLA monoclonal antibody with 125I. The isolation and purification of the doubly labeled (125I-131I) immune complex was performed by affinity chromatography and chromatofocusing successively. When the specific activity of glycoproteins is known, the amount of glycoproteins which bind specifically to the anti-Class II HLA monoclonal antibody can be deduced. In breast carcinoma cells, 1.5 to 2% of the purified glycoproteins bind specifically to the monoclonal antibody, whereas less than 0.3% of normal breast gland cells binds. In contrast, leukemic cells, of which 80 to 90% possess Class II HLA antigens, 2 to 3% of Class II HLA glycoproteins bind specifically with the anti-Class II HLA monoclonal antibody.
D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex / Q000302:isolation & purification; D001940:Breast / Q000276:immunology*; D001943:Breast Neoplasms / Q000276:immunology*; D002846:Chromatography, Affinity; D005260:Female; D006680:HLA Antigens / Q000032:analysis*; D006801:Humans; D007457:Iodine Radioisotopes; D037102:Lectins
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D006680:HLA Antigens; D007457:Iodine Radioisotopes; D037102:Lectins