Characterization of sialyltransferases from serum of normal and hepatoma Mc-29 bearing chickens.
Sialyltransferase was measured in serum of normal and hepatoma Mc-29 bearing chickens. By preparative isoelectric focusing the multiple forms of sialyltransferase from both kind of serums was studied as well. By using influenza virus neuraminidase an attempt was made for partial structural characterization of the sialylation sites in asialofetuin applied as exogenous acceptor for sialyltransferase determination. It was established an elevated serum sialyltransferase activity in tumor bearing chickens with tumor an enzyme form was detected with pI-4.99 identical with an enzyme form described previously in solubilized plasma membrane preparations from hepatoma Mc-29. Monitoring of multiple forms of serum glycosyltransferases may be of value in answering the problem concerning the tissue origin of serum enzymes.
D000818:Animals; D002645:Chickens; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000097:blood / Q000302:isolation & purification; D008114:Liver Neoplasms, Experimental / Q000097:blood / Q000201:enzymology*; D012016:Reference Values; D012799:Sialyltransferases / Q000097:blood* / Q000302:isolation & purification; D014166:Transferases / Q000097:blood*
D007527:Isoenzymes; D014166:Transferases; D012799:Sialyltransferases