Cytosolic redox potential and phosphate transport in the proximal tubule of the rabbit. A study in the isolated perfused tubules.
Recently it has been proposed that cytosolic NAD+ may play a regulatory role in phosphate transport across the renal proximal tubules. To test this thesis, we have examined the effect of altering the cytosolic redox potential (NAD+/NADH) on phosphate flux across the isolated perfused rabbit proximal tubules. The cytosolic redox potential was shifted to a more oxidized state either by changing the substrate in the bathing medium from lactate to pyruvate or by adding methylene blue to the bath medium. On the other hand, cytosolic redox potential was shifted to a more reduced state by changing the substrate in the bath medium from pyruvate to lactate. In either case the phosphate flux across the proximal tubule was unaffected. It was concluded that cytosolic redox potential may not play a significant role in regulating the phosphate transport in renal proximal tubules.
D000818:Animals; D001693:Biological Transport, Active; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism; D008297:Male; D008751:Methylene Blue / Q000494:pharmacology; D009243:NAD / Q000378:metabolism*; D010084:Oxidation-Reduction; D010477:Perfusion; D010710:Phosphates / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism; D011817:Rabbits
D007773:Lactates; D010710:Phosphates; D011773:Pyruvates; D009243:NAD; D008751:Methylene Blue