A low molecular weight extract of bovine serum albumin stimulates rabbit blastocyst cell division and expansion in vitro.
Fraction V bovine serum albumin (BSA) was dissolved in 5% formic acid and filtered through a molecular filter with a cutoff of Mr 10 000. The freeze-dried filtrate stimulated increased cell division of cultured rabbit morulae to blastocysts (up to 8-fold increase in cell number) and increased blastocyst expansion (greater than 2-fold). These results show that some samples of commercial BSA contain an embryonic growth factor of a low molecular weight.
D000818:Animals; D001755:Blastocyst / Q000166:cytology* / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D066298:In Vitro Techniques; D008970:Molecular Weight; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D013268:Stimulation, Chemical
D012710:Serum Albumin, Bovine