Enzyme amplification--a general method applied to provide an immunoassisted assay for placental alkaline phosphatase.
A method is described in which enzyme amplification is used for the quantitative determination of placental alkaline phosphatase. In this particular application, placental alkaline phosphatase is captured on a solid surface by means of a monoclonal antibody. The enzyme is then determined by dephosphorylation of NADP to NAD which activates a strictly NAD-dependent redox cycle leading to the formation of a coloured product.
D000469:Alkaline Phosphatase / Q000032:analysis* / Q000276:immunology; D000911:Antibodies, Monoclonal; D005260:Female; D006801:Humans; D009243:NAD / Q000032:analysis; D009249:NADP / Q000378:metabolism; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy
D000911:Antibodies, Monoclonal; D009243:NAD; D009249:NADP; D000469:Alkaline Phosphatase