title: Substrate specificities of the enzymes of the oleate desaturase system from photosynthetic tissue.
abstract: In the microsomal fraction from young pea (Pisum sativum L.) leaves, the oleoyl moieties from oleoyl-CoA are principally transferred to the sn-2 position of phosphatidylcholine by oleoyl-CoA:1-acyl-lysophosphatidylcholine acyltransferase. The major product of this acyl transfer is 1-palmitoyl(stearoyl)-2-oleoyl phosphatidylcholine. The 1-palmitoyl(stearoyl)-2-oleoyl phosphatidylcholine is subsequently converted into 1-palmitoyl(stearoyl)-2-linoleoyl phosphatidylcholine by the oleate desaturase complex without equilibrating with the bulk membrane phosphatidylcholine pool. Hence, both the acyl transfer to phosphatidylcholine and the subsequent desaturation of oleoyl moieties occur on the sn-2 position of phosphatidylcholine, and there is also a functional coupling of the acyltransferase and oleate desaturase.
mesh_terms: D007887:Fabaceae; D044943:Fatty Acid Desaturases / Q000378:metabolism*; D008861:Microsomes / Q000201:enzymology; D044925:Oxidoreductases Acting on CH-CH Group Donors; D010743:Phospholipids / Q000378:metabolism; D010788:Photosynthesis; D010944:Plants / Q000201:enzymology*; D010946:Plants, Medicinal; D013379:Substrate Specificity
chemical_list: D010743:Phospholipids; D044943:Fatty Acid Desaturases; C021904:Phosphatidylcholine desaturase; D044925:Oxidoreductases Acting on CH-CH Group Donors