Characteristics of methanogens isolated from bovine rumen.
Six strains of methanogens were isolated from 10(-8) and 10(-9) ml of bovine rumen contents. All strains had the morphologic and physiologic characteristics of Methanobrevibacter spp. Four strains required coenzyme M; two did not. Growth of all strains either depended on or was stimulated by a mixture of isobutyric, isovaleric, 2-methylbutyric, and valeric acids. None of the strains reacted with antiserum against the type strain of Methanobrevibacter ruminantium.
D000818:Animals; D002417:Cattle / Q000382:microbiology*; D019605:Euryarchaeota / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*; D008297:Male; D012417:Rumen / Q000382:microbiology*