Analysis of D-penicillamine by gas chromatography utilizing nitrogen--phosphorus detection.
A method is presented for the analysis of the "orphan" drug D-penicillamine (D-Pa), which is used for the treatment of the inherited rare copper metabolism dysfunction known as Wilson's disease, by assaying a derivative of the compound by gas chromatography employing a rubidium sensitized nitrogen--phosphorus detector. Analytical procedures are described for the analyses of residues of D-Pa X HCl salt in animal feed and for the analyses of the salt or free base from aqueous solutions by utilizing a single-step double derivatization with diazomethane--acetone. Stability data for D-Pa X HCl in animal feed and for the free base in water are presented. An ancillary fluorescence derivatization procedure for the analysis of D-Pa in water is also reported.
D000821:Animal Feed / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas / Q000379:methods; D003978:Diazomethane; D004355:Drug Stability; D009682:Magnetic Resonance Spectroscopy; D009584:Nitrogen; D010396:Penicillamine / Q000032:analysis*; D010758:Phosphorus; D013050:Spectrometry, Fluorescence
D010758:Phosphorus; D003978:Diazomethane; D010396:Penicillamine; D009584:Nitrogen