Scanning electron microscopic examination of the secondary constriction of vero cells.
Vero cells (African green monkey kidney in origin) were prepared by the conventional air-drying method and then processed for SEM by a modification of the conductive method based on thiocarbohydrazide-osmium binding. Under SEM, not only metaphase chromosomes but also resting nuclei showed distinct fibres 30 nm in diameter. A few such fibres were found to run across the secondary constriction of the NOR-carrying chromosome.
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure; D002522:Chlorocebus aethiops; D002843:Chromatin / Q000648:ultrastructure*; D002875:Chromosomes / Q000648:ultrastructure*; D007399:Interphase; D007668:Kidney; D008677:Metaphase; D008855:Microscopy, Electron, Scanning; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*
D002843:Chromatin