Feeding of butylated hydroxytoluene to rats caused a rapid decrease in blood coagulation factors II (prothrombin), VII, IX and X.
Male Sprague-Dawley rats were fed a diet containing 1.2% butylated hydroxytoluene (BHT) for 1-7 days, and blood coagulation factors II, VII, VIII, IX and X, and platelet aggregation were measured. The plasma concentrations of factors II, VII, IX and X were significantly reduced in a time-dependent fashion when BHT was administered for 2-7 days and haemorrhages in epididymis were found in rats given BHT for 4-7 days. On the contrary, thrombin-induced and calcium-required aggregation of washed platelets was unchanged throughout the experiment. These results suggest that factors II, VII, IX and X rapidly decrease immediately after the administration of BHT, but hypoaggregability of washed platelets reported previously may be a secondary defect caused by bleeding.
D000818:Animals; D001779:Blood Coagulation Factors / Q000032:analysis*; D002084:Butylated Hydroxytoluene / Q000494:pharmacology* / Q000633:toxicity; D005164:Factor IX / Q000032:analysis; D005167:Factor VII / Q000032:analysis; D005170:Factor X / Q000032:analysis; D006470:Hemorrhage / Q000139:chemically induced; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects; D011516:Prothrombin / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D001779:Blood Coagulation Factors; D002084:Butylated Hydroxytoluene; D005167:Factor VII; D011516:Prothrombin; D005164:Factor IX; D005170:Factor X