3-Hydroxybenzo[a]pyrene glucuronidation by cells: a direct fluorometric assay in culture medium.
A direct fluorometric assay for measuring the glucuronidation by cultured cells using 3-hydroxybenzo[a]pyrene (less than 1 microM) as substrate is described. The method is based on the different characteristics in the fluorescence spectra of 3-hydroxybenzo[a]pyrene and benzo[a]pyrene-3-glucuronide. The analytical procedure operates directly on the culture medium without extraction steps. The sensitivity of detection is 1 nmol glucuronide/1 mumol substrate.
D000818:Animals; D001580:Benzopyrenes / Q000032:analysis* / Q000378:metabolism; D002478:Cells, Cultured; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D012457:Salicylamides / Q000494:pharmacology; D012996:Solutions; D013050:Spectrometry, Fluorescence / Q000379:methods*
D001580:Benzopyrenes; D012457:Salicylamides; D012996:Solutions; C039301:benzo(a)pyrene-3-O-glucuronide; C021338:3-hydroxybenzo(a)pyrene; C031060:salicylamide