Observations on the biochemical basis of ethanol metabolism by human macrophages.
The metabolism of ethanol (0.1 and 1.0 mg/ml) to acetate by blood-monocyte-derived macrophages was only slightly inhibited by pyrazole, 4-iodopyrazole and 3-amino-1,2,4-triazole indicating that it was largely independent of alcohol dehydrogenase and catalase. By contrast, 50-87% of the oxidation of ethanol by these cells was inhibited by carbon monoxide, metyrapone and SKF-525A, all three of which are known to inhibit various species of microsomal cytochrome P-450. The data suggest that most of the ethanol metabolism by blood-monocyte-derived macrophages is mediated via the cytochrome-P-450-dependent microsomal ethanol-oxidising system.
D000640:Amitrole / Q000494:pharmacology; D002248:Carbon Monoxide / Q000494:pharmacology; D002374:Catalase / Q000037:antagonists & inhibitors / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D000431:Ethanol / Q000378:metabolism*; D006801:Humans; D008264:Macrophages / Q000378:metabolism*; D008797:Metyrapone / Q000494:pharmacology; D009249:NADP / Q000378:metabolism; D011335:Proadifen / Q000494:pharmacology; D011720:Pyrazoles / Q000494:pharmacology
D011720:Pyrazoles; D000431:Ethanol; D009249:NADP; D002248:Carbon Monoxide; D011335:Proadifen; D002374:Catalase; D000640:Amitrole; D008797:Metyrapone