Induction of SCEs by some pyrrolizidine alkaloids in V79 Chinese hamster cells co-cultured with chick embryo hepatocytes.
Genotoxicity of heliotrine, monocrotaline, seneciphylline and senkirkine was studied with the sister-chromatid exchange assay in V79 Chinese hamster cells. Exposure to these pyrrolizidine alkaloids, in the presence of co-cultured primary chick embryo hepatocytes, resulted in a high induction of SCEs. It was also shown for heliotrine that co-cultivation with chick embryo hepatocytes resulted in a much higher response than addition of activated rat-liver homogenate to the test medium.
D000818:Animals; D001711:Biotransformation; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002642:Chick Embryo; D006224:Cricetinae; D003412:Cricetulus; D008099:Liver / Q000166:cytology / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D009153:Mutagens; D011763:Pyrrolizidine Alkaloids / Q000378:metabolism / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D009153:Mutagens; D011763:Pyrrolizidine Alkaloids