A compound from uremic plasma and from normal urine isolated by liquid chromatography and identified by nuclear magnetic resonance.
A compound present in normal urine and in ultrafiltrates of uremic plasma in the fraction of so-called "uremic middle molecules" was isolated by liquid chromatography. Preliminary studies, including amino acid analysis, characterization of uronic acids, and ultraviolet spectroscopy, show that the molecule contains glycine, a uronic acid, and an aromatic ring. Characterization by 1H and 13C nuclear magnetic resonance spectrometry shows conclusively that this compound is a double conjugate of glucuronidate--o-hydroxyhippuric acid, which has been previously described by Zimmerman et al., using quite different techniques of isolation and identification (Clin Nephrol 14: 107, 1980; FEBS Lett 129: 237, 1981).
D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange; D005965:Glucuronates / Q000032:analysis; D020723:Glucuronic Acid; D006626:Hippurates / Q000032:analysis; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D013056:Spectrophotometry, Ultraviolet; D014118:Toxins, Biological / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D014511:Uremia / Q000097:blood*
D005965:Glucuronates; D006626:Hippurates; D014118:Toxins, Biological; C026865:uremia middle molecule toxins; C000693:salicylurate; D020723:Glucuronic Acid