title: Platelets, leucocytes and buffy layer vitamin C after surgery.
abstract: The changes in buffy layer (BL) vitamin C concentration following surgical operations were studied in relation to changes in the leucocyte and platelet counts. It was found that the postoperative fall and subsequent changes in BL vitamin C concentration corresponded to changes in the platelet/leucocyte ratio (PLR) in whole blood and consequently in the buffy layer preparations from which vitamin C concentrations were measured. The results of the study showed that the fall in BL vitamin C measured following operations is an artifact of the method used rather than a demand for vitamin C. It highlights the importance of measuring the PLR, and suggests the need for a differential leucocyte count, when undertaking BL vitamin C analysis.
mesh_terms: D000328:Adult; D000368:Aged; D001205:Ascorbic Acid / Q000097:blood* / Q000627:therapeutic use; D001792:Blood Platelets / Q000032:analysis*; D001803:Blood Transfusion; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D007962:Leukocytes / Q000032:analysis*; D007964:Leukocytosis / Q000188:drug therapy / Q000209:etiology; D008297:Male; D008875:Middle Aged; D010976:Platelet Count; D011184:Postoperative Period
chemical_list: D001205:Ascorbic Acid