Application of the Beckman JE6-B Elutriator System in the isolation of human monocyte subpopulations.
Beckman Instruments recently developed an improved version of their JE-6 Elutriator Rotor. At 3000 rpm (867 X g), lymphocytes were eluted from the chamber at flow rates of 10 to 18 ml/min. Small monocytes (362 mu 3) were collected at 18 ml/min over 400 ml while the larger monocytes (395 mu 3) were retained in the chamber until the flow rate was increased to 28 ml/min. These parameters differ from those previously reported by us when we used Beckman's original commercial version of the JE-6 Elutriator Rotor. The new JE-6B rotor required an increase of centrifugal force from 625 to 867 X g generated by an increase in rpm from 2500 to 3000, as well as an increase in flow rates to collect both small (17 to 18 ml/min) and large (26 to 28 ml/min) monocytes. The monocytes collected using the new rotor with the revised protocol have the same physical and functional properties as those collected with the earlier version using our previously published protocol.
D002469:Cell Separation / Q000295:instrumentation*; D002498:Centrifugation / Q000295:instrumentation; D006801:Humans; D009000:Monocytes