Common structural genes for platelet and plasma fibrinogen.
Fibrinogen from plasma was compared with fibrinogen from platelets using two-dimensional electrophoresis. The source of platelet fibrinogen was isolated alpha-granules, thrombin- and collagen-released platelet material. The B beta- and gamma-chains from the different sources showed similar two-dimensional patterns, while gamma'-chains were not observed in platelet fibrinogen preparations. Furthermore, the A alpha-chain could hardly be identified in platelet preparations. When individual fibrinogen was studied in persons heterozygous for genetic B beta- and gamma-chain variants, the two-dimensional variant pattern could be demonstrated in plasma fibrinogen as well as in platelet fibrinogen. This observation strongly indicates that the structural genes for plasma and platelet fibrinogen B beta- and gamma-chains are identical.
D001792:Blood Platelets / Q000032:analysis*; D003594:Cytoplasmic Granules / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005338:Fibrin Fibrinogen Degradation Products / Q000235:genetics; D005340:Fibrinogen / Q000032:analysis / Q000235:genetics*; D005344:Fibrinopeptide A / Q000235:genetics; D005796:Genes; D006801:Humans; D007525:Isoelectric Focusing; D010446:Peptide Fragments; D010949:Plasma / Q000032:analysis*
D005338:Fibrin Fibrinogen Degradation Products; D010446:Peptide Fragments; C035285:fibrinogen Bbeta (1-42); D005344:Fibrinopeptide A; C031564:fibrinogen (gamma 95-264); D005340:Fibrinogen