A simple one-step HPLC procedure for the purification of the NH2-terminal plasmin-derived B beta 1-42 peptide of human fibrinogen.
Simplified procedures were developed to produce and isolate the plasmin-derived B beta 1-42 fragment from human fibrinogen. Peptides generated from fibrinogen by plasmin were separated by reverse phase HPLC chromatography. Two distinct peaks were identified as having amino acid compositions identical to B beta 1-42. The average final yield of HPLC-purified B beta 1-42 was 1.2 mg per 100 mg of fibrinogen. Recovery of the peptide (40-45% when compared to the theoretical yield) was significantly higher than yields obtained by open column techniques demonstrating the advantages of this simple one-step HPLC procedure.
D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005338:Fibrin Fibrinogen Degradation Products / Q000302:isolation & purification*; D005340:Fibrinogen / Q000032:analysis*; D006801:Humans; D010446:Peptide Fragments
D000596:Amino Acids; D005338:Fibrin Fibrinogen Degradation Products; D010446:Peptide Fragments; C035285:fibrinogen Bbeta (1-42); D005340:Fibrinogen