Desorption chemical ionization mass spectrometry of 1-O-alkyl-2-O-acetyl-sn-glycero-3-phosphocholines and analogues.
Ammonia desorption chemical ionization of ether-linked phospholipids of the type 1-O-alkyl-2-O-acetyl-sn-glycero-3-phosphocholine (platelet-activating factors) and a series of analogues revealed a systematic fragmentation pattern that is characteristic for these compounds. The predominant ions included the protonated molecular ion and a series of fragments derived from the molecular ion having the following nominal mass losses: MH-14, MH-42, MH-59, and MH-183. Deuterated ammonia was used to elucidate the nature of several fragments. In addition, desorption chemical ionization was used to quantitate 1-O-hexadecyl-2-O-acetyl-sn-glycero-3-phosphocholine at the nanogram/sample level.
D000641:Ammonia; D055598:Chemical Phenomena; D002621:Chemistry; D007477:Ions; D013058:Mass Spectrometry / Q000379:methods; D010972:Platelet Activating Factor / Q000031:analogs & derivatives / Q000032:analysis*
D007477:Ions; D010972:Platelet Activating Factor; D000641:Ammonia