Hypoxia and angiotensin II infusion redistribute lung blood flow in lambs.
To assess the effects of alveolar hypoxia and angiotensin II infusion on distribution of blood flow to the lung we performed perfusion lung scans on anesthetized mechanically ventilated lambs. Scans were obtained by injecting 1-2 mCi of technetium-labeled albumin macroaggregates as the lambs were ventilated with air, with 10-14% O2 in N2, or with air while receiving angiotensin II intravenously. We found that both alveolar hypoxia and infusion of angiotensin II increased pulmonary vascular resistance and redistributed blood flow from the mid and lower lung regions towards the upper posterior region of the lung. We assessed the effects of angiotensin II infusion on filtration pressure in six lambs by measuring the rate of lung lymph flow and the protein concentration of samples of lung lymph. We found that angiotensin II infusion increased pulmonary arterial pressure 50%, lung lymph flow 90%, and decreased the concentration of protein in lymph relative to plasma. These results are identical to those seen when filtration pressure increases during alveolar hypoxia. We conclude that alveolar hypoxia and angiotensin II infusion both increase fluid filtration in the lung by increasing filtration pressure. The increase in filtration pressure may be the result of a redistribution of blood flow in the lung with relative overperfusion of vessels in some areas and transmission of the elevated pulmonary arterial pressure to fluid-exchanging sites in those vessels.
D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D001794:Blood Pressure; D002199:Capillary Permeability; D005374:Filtration; D000860:Hypoxia / Q000503:physiopathology*; D008168:Lung / Q000098:blood supply* / Q000000981:diagnostic imaging / Q000187:drug effects; D008196:Lymph / Q000378:metabolism; D008833:Microcirculation / Q000503:physiopathology; D011312:Pressure; D011652:Pulmonary Circulation; D011654:Pulmonary Edema / Q000209:etiology; D011877:Radionuclide Imaging; D012756:Sheep; D013668:Technetium Tc 99m Aggregated Albumin; D014655:Vascular Resistance; D014882:Water-Electrolyte Balance / Q000187:drug effects
D013668:Technetium Tc 99m Aggregated Albumin; D000804:Angiotensin II