Ataxia-telangiectasia homo- and heterozygous cells show a normal repair and fixation response to anisotonic NaCl treatment after irradiation.
The effect of anisotonic NaCl treatment on fixation and repair of radiation-induced potentially lethal damage (PLD) was tested in normal human cells and in three homozygous ataxia-telangiectasia (A-T) and two heterozygous A-T cell strains. Fixation of radiation-induced PLD occurred in all cell strains exposed to 0.05, 0.5, or 1.5 mole/liter NaCl solutions immediately after irradiation. This effect was observed in both plateau-phase and exponentially growing normal and A-T cells. When an incubation period at 37 degrees C was introduced between irradiation and the subsequent anisotonic treatment, recovery was observed in both normal and A-T cells strains. These data show that A-T cells are as proficient as normal cells in repairing PLD that is sensitive to anisotonic NaCl treatment. It is proposed that two PLD repair systems may exist, one that is expressed after irradiation in proliferatively arrested cells and another that occurs in plateau-phase as well as exponentially growing cells, and is expressed by the postirradiation treatments described here and by Raaphorst and Azzam (Radiat. Res. 86, 52-66 (1981].
D001260:Ataxia Telangiectasia / Q000235:genetics*; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D004260:DNA Repair / Q000187:drug effects*; D004307:Dose-Response Relationship, Radiation; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D066298:In Vitro Techniques; D009997:Osmotic Pressure; D012965:Sodium Chloride / Q000494:pharmacology*; D012996:Solutions
D012996:Solutions; D012965:Sodium Chloride