Divalent cation dependent conformations of brain calmodulin detected by 1H NMR.
The effects of the Ca2+, Mg2+, Zn2+, Cd2+, Hg2+ and Mn2+ on the conformation of calmodulin (CaM) have been tested by using 400 MHz 1H NMR. In the aromatic region of the spectrum of CaM with a one molar ratio of cation per CaM, Zn2+, Cd2+, Hg2+ and Mn2+ induced spectral changes which were very similar to those seen for Ca2+. However, the Mg2+-induced change was different. These results are consistent with our pharmacological findings on divalent cations.
D000818:Animals; D001679:Biogenic Amines / Q000378:metabolism; D001923:Brain Chemistry; D002104:Cadmium / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002147:Calmodulin / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008345:Manganese / Q000494:pharmacology; D008628:Mercury / Q000494:pharmacology; D051379:Mice; D011487:Protein Conformation; D015032:Zinc / Q000494:pharmacology
D001679:Biogenic Amines; D002147:Calmodulin; D002104:Cadmium; D008345:Manganese; D008628:Mercury; D015032:Zinc; D002118:Calcium