Functional disability of rat splenocytes provoked to lipid peroxidation by cumene hydroperoxide.
Rat splenocytes were provoked to lipid peroxidation in a dose-dependent manner by cumene hydroperoxide. After exposure to cumene hydroperoxide, formation of high molecular weight protein, presumably through cross-linking of lower molecular weight protein, was stimulated in splenocytes as well as in erythrocyte ghosts. The mitogenic response to concanavalin A of splenocytes was remarkably depressed by addition of cumene hydroperoxide to cultures. This depression was due rather to failures of splenocytes in responding to concanavalin A than deactivation of concanavalin A molecules. It is notworthy that the viability of splenocytes was unaffected by cumene hydroperoxide under the culture conditions where the mitogenic response was depressed. The addition of alpha-tocopherol or thiourea could block the depression of mitogenic response by cumene hydroperoxide, indicating that the depressed response to concanavalin A was related to radical formation. Overall evidence suggests that the function of immunocompetent cells can be depressed through lipid peroxidation-associated mechanisms without suffering from lethal damage.
D000818:Animals; D001555:Benzene Derivatives / Q000494:pharmacology*; D003208:Concanavalin A / Q000494:pharmacology; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000096:biosynthesis*; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D013154:Spleen / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism
D001555:Benzene Derivatives; D008054:Lipid Peroxides; D003208:Concanavalin A; C007164:cumene hydroperoxide