Specific binding of [3H] mepyramine to histamine H1-receptors in the sarcolemma from porcine aorta and coronary artery.
We studied the subcellular distribution and the properties of [3H] mepyramine binding in the porcine vascular smooth muscle. A close correlation was observed between the specific binding activity of [3H] mepyramine and the extent of the enrichment of sarcolemmal marker enzyme in 4 subfractions obtained by sucrose density gradient centrifugation of the aortic microsome. In the binding isotherm in the sarcolemmal fractions from the aorta and coronary artery, there was no difference in the Kd value, but the Bmax of the coronary artery was significantly lower than that of the aorta. Thus, there is a single type of high affinity mepyramine binding site in the porcine vascular smooth muscle sarcolemma and the number of H1-receptors of the coronary artery may be smaller than that of the aorta.
D000631:Aminopyridines / Q000378:metabolism*; D000818:Animals; D001011:Aorta / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D003331:Coronary Vessels / Q000378:metabolism*; D007700:Kinetics; D011738:Pyrilamine / Q000378:metabolism*; D011968:Receptors, Histamine / Q000378:metabolism*; D011969:Receptors, Histamine H1 / Q000378:metabolism*; D012508:Sarcolemma / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D013552:Swine
D000631:Aminopyridines; D011968:Receptors, Histamine; D011969:Receptors, Histamine H1; D011738:Pyrilamine