A statistical approach to determine monoclonality after limiting cell plating of a hybridoma clone.
One of the standard methods to isolate a hybridoma clone producing a monoclonal antibody requires successive steps of limiting dilution. The probability of obtaining a monoclonal antibody increases with the number of limiting dilution steps. However, without meticulous visual screening monoclonality is hard to prove. Here we describe a statistical analysis, based on Poisson's approximation, which allows one to calculate the number of hybridoma cells at a given plating efficiency so that when seeded a predictable number of mono-, bi-, etc.-clonal cultures are obtained after the first step of limiting cell plating.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D006825:Hybridomas / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013223:Statistics as Topic
D000911:Antibodies, Monoclonal