title: Identification of phorbol ester binding activity in the particulate and soluble fractions of human fetal tissues.
abstract: The presence of specific binding sites for phorbol esters was demonstrated in various human fetal tissues by binding assays of [3H]12-O-tetradecanoylphorbol-13-acetate (TPA) to particulate and cytosol fractions. Maximal binding activity of the soluble receptor is dependent on calcium and phosphatidylserine. Marked differences in receptor levels were found in various human fetal tissues. However, all the tissues tested contained binding sites for TPA. Particulate receptor concentration was highest in the brain, while the cytosolic receptor concentration was high in brain, stomach and liver. Human fetal tissues bound TPA with a Kd between 10 and 30 nM.
mesh_terms: D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D003600:Cytosol / Q000032:analysis; D005260:Female; D005333:Fetus / Q000032:analysis*; D006801:Humans; D010743:Phospholipids / Q000494:pharmacology; D011247:Pregnancy; D011493:Protein Kinase C; D011955:Receptors, Drug; D011971:Receptors, Immunologic / Q000032:analysis*; D013755:Tetradecanoylphorbol Acetate / Q000378:metabolism
chemical_list: D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D010743:Phospholipids; D011955:Receptors, Drug; D011971:Receptors, Immunologic; C083141:phorbol ester binding protein; C027776:phorbol ester receptor; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate