Induction of self-tolerance and enhanced stress protein synthesis in L-132 cells by cadmium chloride and by hyperthermia.
The effect of heat shock or cadmium treatment on protein synthesis and cell survival in L-132 cells has been examined. After cadmium treatment, the synthesis of a polypeptide of Mr 68000 (P68) was greatly enhanced over that of untreated cells. Besides P68 the synthesis of another polypeptide of Mr 89000 (P89) was also enhanced in heat-shocked cells. Both heat shock and cadmium treatment induced self-tolerance. The kinetics of the synthesis of induced polypeptides correlated well with the development of self-tolerance. The patterns of peptide maps obtained after partial proteolytic digestion from P68 induced in cadmium-treated and heat-shocked cells were virtually identical. However, neither heat-shocked cells did not confer cadmium tolerance nor did cadmium-treated cells induce thermotolerance.
D000199:Actins / Q000096:biosynthesis; D000818:Animals; D002104:Cadmium / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D007739:L Cells; D008099:Liver / Q000378:metabolism; D051379:Mice; D010446:Peptide Fragments / Q000032:analysis
D000199:Actins; D006360:Heat-Shock Proteins; D010446:Peptide Fragments; D002104:Cadmium