title: A simple two-step procedure for the preparation of the first component of human complement (C1) in its native form.
abstract: Native human C1 was purified from fresh human serum by affinity chromatography on protein A-bound Sepharose in the presence of 4-nitrophenyl-4-guanidinobenzoate hydrochloride (NPGB) taking advantage of the successive binding of IgG to protein A followed by C1 binding to IgG. After elution the C1 preparation contained IgG as a major contaminant as shown by SDS-PAGE. C1 was further purified by gel filtration. The yield of C1 was 12% and less than 4% of this C1 was activated during purification as assessed by a C4 consumption assay.
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods; D003172:Complement C1 / Q000302:isolation & purification*; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D011485:Protein Binding; D013205:Staphylococcal Protein A / Q000378:metabolism
chemical_list: D003172:Complement C1; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A