title: Variations in ADP-ribosylation of nuclear scaffold proteins during the HeLa cell cycle.
abstract: Cell cycle variations in ADP-ribosylation of nuclear scaffold proteins were determined. Nuclei of synchronized cells were isolated and labeled with [32P]NAD before nuclear scaffolds were obtained by digestion of DNA with DNase I and extraction of proteins with 2M NaCl. Autoradiograms revealed the three groups of "lamins" and a species identified as poly (ADP-ribose) polymerase to be the primary ADP-ribosylated proteins. The patterns of modification of nuclear scaffold proteins displayed similar features through the cell cycle. Radioactivity in the lamins increased from 20% in early-S phase to 40% in G1 phase of the next cell cycle.
mesh_terms: D000227:Adenine Nucleotides / Q000494:pharmacology; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D001549:Benzamides / Q000494:pharmacology; D002453:Cell Cycle; D002467:Cell Nucleus / Q000378:metabolism*; D015226:Dinucleoside Phosphates; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D034882:Lamins; D009243:NAD / Q000378:metabolism; D009698:Nucleoproteins / Q000378:metabolism*; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*
chemical_list: D000227:Adenine Nucleotides; D001549:Benzamides; D015226:Dinucleoside Phosphates; D034882:Lamins; D009698:Nucleoproteins; D009702:Nucleoside Diphosphate Sugars; D009243:NAD; D000246:Adenosine Diphosphate Ribose; C020733:diadenosine tetraphosphate; C025160:3-aminobenzamide