Cleavage of the polysialosyl units of brain glycoproteins by a bacteriophage endosialidase. Involvement of a long oligosaccharide segment in molecular interactions of polysialic acid.
Polysialosyl chains containing alpha 2-8-linked N-acetylneuraminic acid have been suggested to modulate the biological activity of a neural cell adhesion molecule. Polysialosyl glycopeptides isolated from developing brain were incubated with a bacteriophage containing endosialidase. Sialic acid oligomers up to 7 residues long were liberated both from the glycopeptides and colominic acid. The substrate specificity of the endosialidase was studied with sialic acid oligomers of different sizes prepared from colominic acid. It was found that the endosialidase required the simultaneous presence adjacent to the site of cleavage a minimum of 3 sialic acid residues on the distal side and a minimum of 5 sialic acid residues on the proximal (reducing end) side. From the fragments liberated by the enzyme the existence of polysialic acid chains up to at least 12 residues long in the glycopeptides were concluded. This was also supported by the interaction of the glycopeptides with a meningococcal group B polysaccharide antiserum, which was found to require 10 residues or more for binding. The results indicate that the brain polysialosyl glycopeptides contain a long polysialic acid segment, which is also specifically needed for certain molecular interactions. The implications of the findings for the biological properties of the neural cell adhesion molecule are discussed.
D000818:Animals; D000907:Antibodies, Bacterial; D016667:Bacterial Capsules; D001923:Brain Chemistry; D002448:Cell Adhesion; D002855:Chromatography, Thin Layer; D003090:Coliphages / Q000201:enzymology*; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D009439:Neuraminidase / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000276:immunology; D051381:Rats; D012794:Sialic Acids / Q000378:metabolism*; D013379:Substrate Specificity
D000907:Antibodies, Bacterial; D006023:Glycoproteins; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; D012794:Sialic Acids; C039455:capsular polysaccharide, meningococcal group B; C021319:polysialic acid; C014420:colominic acid; C035355:endo-N-acetylneuraminidase; D009439:Neuraminidase