Isolation and primary structure of novel neurointermediate pituitary peptides derived from the C-terminal of the rat vasopressin-neurophysin precursor (propressophysin).
Four novel peptides were isolated from rat neurointermediate lobes by gel filtration and high-pressure liquid chromatography. Analyses of amino acid composition and sequence showed that all four peptides were derived from the C-terminal portion of propressophysin (CPP); they were identified as the glycopeptides CPP 1-19, CPP 1-20, CPP 22-37 and CPP 22-39. Processing of CPP 1-39 could thus produce the four isolated peptides by specific post-Arg or post-Leu cleavages.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001127:Arginine Vasopressin / Q000032:analysis*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D008297:Male; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D009481:Neurophysins / Q000032:analysis*; D010121:Oxytocin; D010902:Pituitary Gland / Q000032:analysis*; D011498:Protein Precursors / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D000596:Amino Acids; D009419:Nerve Tissue Proteins; D009481:Neurophysins; D011498:Protein Precursors; D001127:Arginine Vasopressin; D010121:Oxytocin