An ammonium sulphate fraction from rabbit reticulocytes increases the release of proteins from rat liver mitochondria.
Incubation of [35S]methionine labeled mitochondria from rat liver with rabbit reticulocyte lysate under the same conditions as those used in the import of mitochondrial protein precursors results in the release of mitochondrial proteins to the medium. Fractionation of the lysates with ammonium sulphate yields a fraction, essentially free of haemoglobin, which exhibits higher activity for the release of mitochondrial proteins than the starting lysate. The fraction has a molecular mass of greater than 10 kDa and is heat-sensitive. The release is insensitive to inhibitors of reticulocyte lipoxygenase.
D000645:Ammonium Sulfate; D000818:Animals; D002457:Cell Extracts / Q000494:pharmacology*; D006358:Hot Temperature; D008715:Methionine / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects*; D008970:Molecular Weight; D011506:Proteins / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D012156:Reticulocytes / Q000032:analysis*; D014020:Tissue Extracts / Q000494:pharmacology*
D002457:Cell Extracts; D011506:Proteins; D014020:Tissue Extracts; D008715:Methionine; D000645:Ammonium Sulfate