Evaluation of microtitre plates for the routine assay of oestrogen and progesterone receptors in tissue extracts.
A time- and material-saving assay of oestrogen and progesterone receptors in tissue extracts using enzyme-linked immunosorbent assay quality microtitre plates and microtitre-compatible equipment is described. The results from simultaneous determinations in microtitre plates and in conventional tubes show the former to yield higher receptor values. Furthermore, the results of a large series of measurements in microtitre plates compare favourably with previously obtained results from assays in tubes.
D001943:Breast Neoplasms / Q000032:analysis*; D003600:Cytosol / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation; D005260:Female; D006801:Humans; D011960:Receptors, Estrogen / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis*
D011960:Receptors, Estrogen; D011980:Receptors, Progesterone