Irreversible inhibition of trypsin by TLCK. A continuous method for kinetic study of irreversible enzymatic inhibitors in the presence of substrate.
This work presents a kinetic study on irreversible inhibition of trypsin by TLCK, using a new experimental approach. The method consists of the incubation of the enzyme with an irreversible inhibitor in the presence of a substrate which allows enzyme turnover as well as continuous measurement of the appearance of the product, a simultaneous change in the initial concentrations of the irreversible inhibitor and enzyme being undertaken, though a constant ratio between the latter, is maintained. This new approach enables the kinetic constants for TLCK, k2 and K1, to be determined.
D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology*; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D007700:Kinetics; D008433:Mathematics; D011485:Protein Binding; D014107:Tosyllysine Chloromethyl Ketone / Q000494:pharmacology*; D014361:Trypsin Inhibitors
D000590:Amino Acid Chloromethyl Ketones; D014361:Trypsin Inhibitors; D014107:Tosyllysine Chloromethyl Ketone