title: The structure of nucleosome core particles as revealed by difference Raman spectroscopy.
abstract: Raman spectra have been observed of nucleosome core particles (I) prepared from chicken erythrocyte chromatin, its isolated 146 bp DNA (II), and its isolated histone octamer (H2A+H2B+H3+H4)2 (III). By examining the difference Raman spectra, (I)-(II), (I)-(III), and (I)-(II)-(III), several pieces of information have been obtained on the conformation of the DNA moiety, the conformation of the histone moiety, and the DNA-histone interaction in the nucleosome core particles. In the nucleosome core particles, about 15 bp (A.T rich) portions of the whole 146 bp DNA are considered to take an A-form conformation. These are considered to correspond to its bent portions which appear at intervals of 10 bp.
mesh_terms: D000818:Animals; D002645:Chickens; D004247:DNA; D004912:Erythrocytes / Q000648:ultrastructure; D006657:Histones; D009707:Nucleosomes / Q000648:ultrastructure*; D013059:Spectrum Analysis, Raman
chemical_list: D006657:Histones; D009707:Nucleosomes; D004247:DNA