Cholesterol transport in mesenteric lymph of the preruminant calf: contribution of cholesterol transposition from blood to lymph.
Eight preruminant male calves were prepared surgically with lymphatico-venous shunts and re-entrant gallbladder to proximal duodenum shunts. Liquid diets were formulated to contain 12.5% dried skim milk (SM) or 10.5% SM to which was added 2% soybean oil (SBO), milk fat (MF) or beef tallow (T). Two calves were assigned to each dietary treatment. Transposition of cholesterol from blood capillaries to intestinal lymph was determined by injection of 100 microCi [4-14C]-cholesterol into the blood of calves at feeding time. To avoid recirculation of [4-14C]-cholesterol via the enterohepatic circulation, bile was diverted and replaced with bile from a donor calf fed an identical diet. For the SM, SBO, MF and T diets, respectively, cholesterol transposed from capillaries was 44, 61, 36 and 48% of the cholesterol transported in the mesenteric lymph. When cholesterol synthesis in response to test diets was calculated, we found that intestinal cholesterol synthesis is less when calves are fed SM or SBO than when fed T or MF.
D000042:Absorption; D000818:Animals; D000833:Animals, Suckling / Q000378:metabolism; D001692:Biological Transport; D002417:Cattle; D002784:Cholesterol / Q000096:biosynthesis / Q000097:blood / Q000378:metabolism*; D004041:Dietary Fats / Q000378:metabolism*; D004386:Duodenum / Q000378:metabolism; D008196:Lymph / Q000378:metabolism*; D008297:Male; D008643:Mesentery / Q000378:metabolism; D013997:Time Factors
D004041:Dietary Fats; D002784:Cholesterol