2-Chloroadenosine inhibits complement-induced reactive oxygen metabolite production and recovery of human polymorphonuclear leucocytes attacked by complement.
Luminol-enhanced chemiluminescence demonstrated that 2-chloroadenosine inhibited complement-induced reactive oxygen metabolite production in human polymorphonuclear leucocytes. This inhibition was reversed by 8-phenyltheophylline. Binding of a [125I]-labelled monoclonal antibody against C9 to human PMN demonstrated removal of membrane attack complexes from the cell membranes, and in addition indicated inhibition of this process by 2-chloroadenosine. This compound also increased the percentage of complement-induced cell lysis of polymorphonuclear leucocytes.
D015762:2-Chloroadenosine; D000241:Adenosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000911:Antibodies, Monoclonal; D002462:Cell Membrane / Q000187:drug effects; D003167:Complement Activation; D003169:Complement Inactivator Proteins; D006801:Humans; D007075:Immunoglobulin M / Q000494:pharmacology; D008163:Luminescent Measurements; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D010100:Oxygen / Q000097:blood*
D000911:Antibodies, Monoclonal; D003169:Complement Inactivator Proteins; D007075:Immunoglobulin M; D015762:2-Chloroadenosine; D000241:Adenosine; D010100:Oxygen