Determination of ketone body kinetics using a D-(-)-3-hydroxy[4,4,4-2H3]butyrate tracer.
In studies where D-(-)-3-hydroxy[4,4,4-2H3]butyrate is employed as isotopic tracer in vivo, we have described a selected ion monitoring, gas-liquid chromatography-mass spectrometry micromethod which measures [2H3] tracer enrichment in 3-hydroxybutyrate and acetoacetate from 300-microliters blood samples. For plasma samples in the physiologic range, intra- and interassay precisions for each ketone averaged better than +/- 1% and +/- 2%, respectively. The use of the method was validated by comparing kinetic data obtained with the above tracer with simultaneous flux data obtained with conventional D-(-)-3-hydroxy[3-14C]butyrate tracer in five fasted rats.
D020155:3-Hydroxybutyric Acid; D000090:Acetoacetates / Q000096:biosynthesis / Q000097:blood; D000818:Animals; D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry; D006885:Hydroxybutyrates / Q000097:blood; D007657:Ketone Bodies / Q000097:blood / Q000378:metabolism*; D007700:Kinetics; D008832:Microchemistry; D051381:Rats; D011919:Rats, Inbred Strains
D000090:Acetoacetates; D006885:Hydroxybutyrates; D007657:Ketone Bodies; C016635:acetoacetic acid; D003903:Deuterium; D020155:3-Hydroxybutyric Acid