title: Effects of ethanol ingestion on amino acid uptake in the dog liver in vivo.
abstract: The effect of ethanol ingestion on the uptake of labeled amino acids was studied in the in situ autoperfused dog liver. Ethanol was administered orally, as a 15% water solution, in a dose of 4 g/kg body weight/day as the only source of water for 2 days. Amino acid uptake was measured in anesthetized dogs by means of the single-passage, multiple-tracer dilution technique. In control animals, hepatic uptake of 14C-glycine, 3H-alpha-aminoisobutyric acid (3H-AIB) and 3H-L-leucine were 50, 15 and 66%, respectively. In the ethanol-treated dogs, glycine and AIB uptake was reduced by 70 and 63%, respectively. L-leucine uptake was reduced by only 23%. The plasma concentration of the naturally occurring amino acids was significantly increased after ethanol treatment, probably due to a reduced influx into hepatocytes. Simultaneous measurements of hemodynamic parameters showed a significant increase in the portal vein pressure of ethanol-treated animals, whereas the portal vein blood flow and hepatic extracellular volume were unaffected.
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000621:Aminoisobutyric Acids / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002250:Carbon Radioisotopes; D004285:Dogs; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D005998:Glycine / Q000097:blood / Q000378:metabolism; D006439:Hemodynamics / Q000187:drug effects; D007930:Leucine / Q000097:blood / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D012979:Sodium Radioisotopes; D014316:Tritium
chemical_list: D000596:Amino Acids; D000621:Aminoisobutyric Acids; D002250:Carbon Radioisotopes; D012979:Sodium Radioisotopes; D014316:Tritium; C100049:2-aminoisobutyric acid; D000431:Ethanol; D007930:Leucine; D005998:Glycine