title: Chain-shortening of erucic acid and microperoxisomal beta-oxidation in rat small intestine.
abstract: The ability of rat small intestine to chain-shorten C22:1 fatty acids was investigated. Radioactive chain-shortened products, mainly C18:1, were demonstrated in intestinal-lymph lipids after intraluminal injection of [14-14C]erucic acid. Chain-elongation to C24:1 was also observed. Adaptation to a diet containing C22:1 fatty acids (partially hydrogenated-marine-oil diet) slightly increased the percentage of chain-shortened products. Microperoxisomal beta-oxidation activity, measured as CN(-)-insensitive palmitoyl-CoA-dependent NAD+ reduction, was detected in a microperoxisome-enriched fraction from mucosal scrapings. This activity was increased 1.9-fold by a soya-bean-oil diet, and 2.7-fold by a diet containing partially hydrogenated marine oil.
mesh_terms: D000818:Animals; D004883:Erucic Acids / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D005229:Fatty Acids, Monounsaturated; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism*; D050356:Lipid Metabolism; D008196:Lymph / Q000378:metabolism; D008297:Male; D008830:Microbodies / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009821:Oils / Q000378:metabolism; D010084:Oxidation-Reduction; D010171:Palmitoyl Coenzyme A / Q000494:pharmacology; D010938:Plant Oils; D000074262:Rapeseed Oil; D051381:Rats; D011919:Rats, Inbred Strains; D013024:Soybean Oil
chemical_list: D004883:Erucic Acids; D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D005231:Fatty Acids, Unsaturated; D009821:Oils; D010938:Plant Oils; D000074262:Rapeseed Oil; D009243:NAD; D010171:Palmitoyl Coenzyme A; D013024:Soybean Oil