New method of testing for carbohydrate absorption in man. Xylose and sucrose absorption; effects of sucrase inhibition.
Absorption of carbohydrate was quantitated in 49 subjects without disease of the small bowel using a new technique for ileal perfusion. A double-lumen tube with an attached balloon was inserted retrograde through the colon and used to quantify arrival in the ileum of D-xylose and a nonabsorbable marker which had been taken orally. In the same way, absorption of sucrose and the effects of an inhibitor of alpha-glucosidase were also studied. Insertion of the assembly through the colon and intubation of the terminal ileum was usually possible within 30 min; we have designated the technique, endoscopic retrograde bowel insertion (ERBI). The test meals were 500 ml of water containing either 25 g D-xylose and 5 g polyethylene glycol (PEG 4000), or 100 g sucrose with 5 g PEG. Sucrose meals also contained 0, 100, or 200 mg of an inhibitor of alpha-glucosidase (BAYg5421). At the end of a 5-hr test period, the ratio of recovery of D-xylose relative to that of PEG indicated that 69% of D-xylose was absorbed. Five-hour urinary excretion of D-xylose was 31% of that ingested, or 45% of the D-xylose which was absorbed. Sucrose was recovered in ileal samples only when administered together with inhibitor. Rates of sucrose absorption with BAYg5421, 100 and 200 mg, were 75% and 65%, respectively. The perfusion technique of ERBI is a rapid and reproduceable approach to the distal small intestine of man which could be of value in the investigation of intestinal absorption.
D020909:Acarbose; D000293:Adolescent; D000328:Adult; D000368:Aged; D003113:Colonoscopy; D004040:Dietary Carbohydrates / Q000378:metabolism*; D005260:Female; D006801:Humans; D007082:Ileum; D007408:Intestinal Absorption / Q000187:drug effects; D007441:Intubation, Gastrointestinal / Q000379:methods; D008297:Male; D008875:Middle Aged; D011092:Polyethylene Glycols; D013393:Sucrase / Q000037:antagonists & inhibitors*; D013395:Sucrose / Q000378:metabolism; D014312:Trisaccharides / Q000494:pharmacology; D014994:Xylose / Q000378:metabolism
D004040:Dietary Carbohydrates; D014312:Trisaccharides; D011092:Polyethylene Glycols; D013395:Sucrose; D014994:Xylose; D013393:Sucrase; D020909:Acarbose