title: Effect of absorbed and nonabsorbed anti-H-2 antiserum on mouse blastocysts.
abstract: Conventional antisera and monoclonal antibodies have been used to demonstrate the presence of H-2 antigens on preimplantation mouse embryos. In this study, the specificity of previously used anti-H-2 conventional antisera was tested by absorption of the antisera on spleen lymphocytes. It was found that absorption of the anti-H-2 antisera removed all of the antibody reactive with lymphocytes, but only about half of the antibody reactive with embryos. The effect of absorbed and nonabsorbed anti-H-2 antisera on mouse blastocysts was tested both by cytotoxicity assays and by electron microscopy. The results suggest that conventional antisera do indeed detect H-2 antigens on mouse embryos, but they may detect other, as yet undefined, antigens as well.
mesh_terms: D000042:Absorption; D000818:Animals; D001755:Blastocyst / Q000187:drug effects* / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D006183:H-2 Antigens / Q000276:immunology*; D007106:Immune Sera / Q000494:pharmacology*; D051379:Mice; D013936:Thymidine / Q000378:metabolism
chemical_list: D006183:H-2 Antigens; D007106:Immune Sera; D004247:DNA; D013936:Thymidine