Effect of free fatty acids on renal metabolism of very low density lipoprotein triglyceride.
Metabolism of very low density lipoprotein-triglyceride (VLDL-TG) was studied in the isolated perfused rat kidney (IPRK). Recirculating perfusate consisted of Krebs-Henseleit bicarbonate buffer containing 6% defatted albumin and VLDL-TG which has been labelled in vitro with 14C-tripalmitin. Results show that the IPRK catabolizes VLDL-TG. Renal removal of VLDL-TG from perfusate was impaired in the presence of 1.0 mM palmitate. A lower palmitate concentration, 0.4 mM, was without effect. It is concluded that high concentrations of circulating free fatty acids impair renal metabolism of fatty acids derived from VLDL-TG.
D000818:Animals; D002788:Cholesterol Esters / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008079:Lipoproteins, VLDL / Q000378:metabolism*; D008297:Male; D009392:Nephrectomy; D010168:Palmitates / Q000494:pharmacology; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D014280:Triglycerides / Q000378:metabolism*
D002788:Cholesterol Esters; D004075:Diglycerides; D005230:Fatty Acids, Nonesterified; D008079:Lipoproteins, VLDL; D010168:Palmitates; D010743:Phospholipids; D014280:Triglycerides; C005859:tripalmitin