The induction of chromosome condensation in tsBN2, a temperature-sensitive mutant of BHK21, inhibited by the calmodulin antagonist, W-7.
The induction of premature chromosome condensation (PCC) in tsBN2 cells, a temperature-sensitive (ts) mutant of BHK21/13 which shows PCC at the non-permissive temperature, was almost completely inhibited by 40 microM W-7, an antagonist of calmodulin. The mitotic phosphorylation of histone H1 and H3 was also inhibited by W-7. W-5, a chlorine-deficient analogue of W-7 and which interacts weakly with calmodulin, did not inhibit the induction of PCC, even at a dose of 80 microM. The content of calmodulin in tsBN2 cells was increased by a temperature shift to 40.5 degrees C. All these results suggested that calmodulin is required for the chromosome condensation.
D000818:Animals; D002147:Calmodulin / Q000096:biosynthesis / Q000502:physiology*; D002460:Cell Line; D002875:Chromosomes / Q000187:drug effects / Q000378:metabolism*; D006224:Cricetinae; D006657:Histones / Q000378:metabolism; D007668:Kidney; D008938:Mitosis; D009154:Mutation; D010766:Phosphorylation; D013449:Sulfonamides / Q000494:pharmacology*; D013696:Temperature
D002147:Calmodulin; D006657:Histones; D013449:Sulfonamides; C017967:W 7; C034700:N-(6-aminohexyl)-1-naphthalenesulfonamide