mRNA transcripts of several plant genes are polyadenylated at multiple sites in vivo.
We have analyzed the polyadenylation sites for the small subunit of ribulose bisphosphate carboxylase and chlorophyll a/b binding protein genes of Petunia (Mitchell) and the bronze gene of Zea mays. Sequence analysis of multiple cDNA clones revealed that polyadenylation of the transcripts occurred at either 2 or 3 sites for all three groups of genes. In the examples where 3 polyadenylation sites were detected, the middle site was the one predominantly used. Putative polyadenylation signals preceding the poly A tails diverged significantly from the animal consensus sequence AATAAA. In all the genes examined the first A residue in the poly A tail of the cDNA clones corresponded to an A residue in the homologous genomic sequence.
D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics; D002734:Chlorophyll / Q000378:metabolism; D005796:Genes; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D011061:Poly A / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000235:genetics*; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*
D002352:Carrier Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D002734:Chlorophyll; D011061:Poly A; D012273:Ribulose-Bisphosphate Carboxylase