Determination of the concentration and specific activity of acetone in biological fluids.
The concentration of acetone dissolved in liver perfusion medium was determined by injection of the sample into a gas chromatograph equipped with a Carbopack/Carbowax-packed glass column. Interference from labile acetoacetate which readily decomposes to acetone was eliminated by treating the samples with NaBH4 prior to the analysis. Acetone was detected and quantified as 2-propanol. Separation of labeled 2-propanol in the sample by high-performance liquid chromatography allowed the determination of its specific activity. These methods make possible the convenient and rapid determination of acetone concentration and specific activity in biological samples.
D000433:1-Propanol / Q000032:analysis; D000090:Acetoacetates / Q000032:analysis; D000096:Acetone / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D000818:Animals; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008099:Liver / Q000378:metabolism; D010477:Perfusion; D051381:Rats
D000090:Acetoacetates; D000096:Acetone; C016635:acetoacetic acid; D000433:1-Propanol