Isolation and characterization of syncytiotrophoblast plasma membrane from human placenta.
Human full-term syncytiotrophoblast plasma membranes isolated by mechanical procedures (sieving and ultrasonic disintegration), purified by phase centrifugation, form a single band of 1.052 +/- 0.002 g/ml density in percoll gradient. The purity of the preparation was assessed by electron microscopy, enzyme analysis and beta 2-microglobulin determination.
D002458:Cell Fractionation; D002462:Cell Membrane / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient; D005260:Female; D006801:Humans; D008854:Microscopy, Electron; D010920:Placenta / Q000648:ultrastructure*; D011247:Pregnancy; D014327:Trophoblasts / Q000648:ultrastructure*