Analysis of [3H]spiperone binding sites in the rat striatum and frontoparietal cortex by means of quantitative receptor autoradiography after inactivation of dopamine receptors by N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline in vivo: selective protection by sulpiride in the striatum.
By means of quantitative receptor autoradiography in combination with inactivation of dopamine (DA) receptors by N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ), it was demonstrated in the male rat that [3H]spiperone predominantly labels D2 receptors in the striatum, nucleus accumbens and tuberculum olfactorium, and predominantly non-DA receptors in the frontoparietal cortex, probably mainly serotonin type 2 (5-HT2) receptors in layer IV. Furthermore, the [3H]spiperone-labelled D2 receptors found all over the dorsal and ventral striatum appear to be similarly inactivated by EEDQ and protected by the D2 antagonist (+/-) sulpiride but not the D1 antagonist SCH 23390, indicating a homogeneity of the striatal D2 receptors.
D000818:Animals; D001345:Autoradiography; D002090:Butyrophenones / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D003342:Corpus Striatum / Q000378:metabolism*; D008297:Male; D011804:Quinolines / Q000494:pharmacology; D051381:Rats; D011954:Receptors, Dopamine / Q000378:metabolism*; D013134:Spiperone / Q000378:metabolism*; D013469:Sulpiride / Q000494:pharmacology
D002090:Butyrophenones; D011804:Quinolines; D011954:Receptors, Dopamine; D013134:Spiperone; C000470:EEDQ; D013469:Sulpiride