[Migration of polynuclear neutrophils in vivo. Standardization of the technic].
The migration of neutrophils across a skin barrier is a commonly employed method for the study of the inflammatory response. This report aims at comparing the leukocyte migration in the presence of two different chemoattractants : autologous serum and pool of serum. The reproducibility of this method has proved to be increasing by using duplicate skin chambers in 30 healty volunteers. The neutrophil migration with the pool of serum appears more homogeneous than with autologous serum. These results permit to obtain a standard curve of the neutrophil migration using a pool of serum.
D002464:Cell Migration Inhibition / Q000379:methods / Q000592:standards*; D006801:Humans; D009504:Neutrophils / Q000276:immunology*; D010539:Permeability; D012867:Skin
Contribution à l'étude de la migration des polynucléaires neutrophiles in vivo. Standardisation de la technique.