Changes in brain energy metabolism and protein synthesis following transient bilateral ischemia in the gerbil.
The time course of the reduction in brain protein synthesis following transient bilateral ischemia in the gerbil was characterized and compared with changes in a number of metabolites related to brain energy metabolism. The recovery of brain protein synthesis was similar following ischemic periods of 5, 10, or 20 min; in vitro incorporation activity of brain supernatants was reduced to approximately 10% of control at 10 or 30 min recirculation, showed slight recovery at 60 min, and returned to 60% of control activity by 4 h. Protein synthesis activity was indistinguishable from control at 24 h. One minute of ischemia produced no detectable effect on protein synthesis measured after 30 min reperfusion; longer periods of ischemia resulted in progressive inhibition, with 5 min producing the maximal effect. Pentobarbital (50 mg/kg) increased by 1-2 min the threshold ischemic duration required to produce a given effect. Whereas most metabolites recovered quickly following 5 min ischemia, glycogen showed a delayed recovery comparable to that seen for protein synthesis. These results are discussed in relation to possible mechanisms for the coordinate regulation of brain energy metabolism and protein synthesis. An improved method for the fluorimetric measurement of guanine nucleotides is described.
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism*; D004734:Energy Metabolism; D005849:Gerbillinae; D006003:Glycogen / Q000378:metabolism; D006150:Guanine Nucleotides / Q000378:metabolism; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008297:Male; D009249:NADP / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D010424:Pentobarbital / Q000494:pharmacology; D010725:Phosphocreatine / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid
D000227:Adenine Nucleotides; D006150:Guanine Nucleotides; D007773:Lactates; D009419:Nerve Tissue Proteins; D011773:Pyruvates; D010725:Phosphocreatine; D019344:Lactic Acid; D009249:NADP; D019289:Pyruvic Acid; D006003:Glycogen; D010424:Pentobarbital