[Purification of dopamine-beta-monooxygenase and extremely acidic, copper-containing proteins from the adrenal medulla. Extremely acidic, copper-containing proteins as electron donors for dopamine-beta-monooxygenase].
A procedure for purification of two copper-containing proteins from bovine adrenal medulla has been developed. The method is based on the extraction of proteins with Triton X-100, DEAE-cellulose chromatography and fractionation with polyethylene glycol. The yield of dopamine-beta-monooxygenase and extremely acidic copper-containing protein per 1 kg of brain medullar tissue is 100 and 150 mg, correspondingly. The ability of the reduced extremely acidic copper-containing protein to act as an electron donor in the reactions of side chain hydroxylation of tyramine and dopamine catalyzed by dopamine-beta-monooxygenase was demonstrated. The kinetics of the products formation and oxygen consumption in the course of these reactions and the specific activities of dopamine-beta-monooxygenase were compared, using the reduced extremely acidic copper-containing protein, ascorbate and ferrocyanide as cosubstrates.
D000313:Adrenal Medulla / Q000201:enzymology* / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D002417:Cattle; D004299:Dopamine beta-Hydroxylase / Q000302:isolation & purification* / Q000378:metabolism; D004579:Electron Transport; D005295:Ferrocyanides / Q000378:metabolism; D006900:Hydroxylation; D007700:Kinetics; D008667:Metalloproteins / Q000302:isolation & purification* / Q000378:metabolism; D010101:Oxygen Consumption
D005295:Ferrocyanides; D008667:Metalloproteins; D004299:Dopamine beta-Hydroxylase; C020354:hexacyanoferrate II; D001205:Ascorbic Acid
Ochistka dofamin-beta-monooksigenazy i kraĭne kislogo med'soderzhashchego belka iz mozgovogo sloia nadpochechnikov. Kraĭne kislyI med'soderzhashchiĭ belok kak donor élektronov dlia dofamin-beta-monooksigenazy.