Effects of serum and serum components on the binding of DNA to thioglycollate-stimulated mouse peritoneal macrophages.
Binding of ssDNA and dsDNA to thioglycollate-stimulated peritoneal macrophages derived from normal CBA mice was increased not only by ssDNA-specific antibody but also by albumin and gamma-globulin preparations. Fresh mouse serum inhibited ssDNA binding, even if the ssDNA and serum were preincubated at 37 degrees C. Heat decomplemented mouse serum also depressed DNA binding. These results are significant to interpretations of DNA clearance rate data from serum or blood, and subsequent inferences concerning blood DNA levels, autoimmune response, and systemic lupus erythematosus.
D000818:Animals; D001769:Blood; D004247:DNA / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008808:Mice, Inbred CBA; D010537:Peritoneum / Q000166:cytology; D012709:Serum Albumin / Q000494:pharmacology; D012712:Serum Globulins / Q000494:pharmacology; D013864:Thioglycolates / Q000494:pharmacology*
D004277:DNA, Single-Stranded; D012709:Serum Albumin; D012712:Serum Globulins; D013864:Thioglycolates; D004247:DNA