Histamine receptors on leukocytes: the binding of histamine serum albumin conjugates is non-specific.
It is commonly held that certain leukocyte sub-populations adhere selectively to histamine-serum albumin conjugates. A large number of soluble histamine, ethylamine and dimethylaminopropylamine conjugates were synthesised and labelled with fluorescein isothiocyanate (FITC). Although a number of conjugates bound to mononuclear cells and granulocytes our data showed this binding to be non-specific. The binding of histamine conjugates to leukocytes was not inhibited by preincubation of cells with free histamine at concentrations of up to 10(-1) M nor was it pH sensitive. In addition, conjugates of ethylamine and dimethylaminopropylamine showed a similar binding pattern to that of histamine conjugates. We suggest that the observed binding may be due to modifications to the net charge of the carrier protein molecule.
D002352:Carrier Proteins / Q000378:metabolism; D003959:Diamines / Q000378:metabolism; D005021:Ethylamines / Q000378:metabolism; D006632:Histamine / Q000378:metabolism*; D006801:Humans; D007962:Leukocytes / Q000378:metabolism* / Q000648:ultrastructure; D008875:Middle Aged; D011968:Receptors, Histamine / Q000378:metabolism*; D012709:Serum Albumin / Q000378:metabolism*
D002352:Carrier Proteins; D003959:Diamines; D005021:Ethylamines; D011968:Receptors, Histamine; D012709:Serum Albumin; D006632:Histamine; C012928:3-dimethylaminopropylamine; C041564:ethylamine