In vivo acetylation of homocholine and beta-methylcholine in rat brain.
A nitrogen phosphorus-gas chromatographic procedure was modified to determine the extent of in vivo acetylation of the choline analogs homocholine and beta-methylcholine. Infusion of homocholine (18 mumoles) for 2 hours into the lateral ventricle of the rat produced 2.3 nmoles/gram of acetylhomocholine which represented 0.035% of the detected homocholine. Infusion of the same quantity of beta-methylcholine produced 1.0 nmole/gram of acetyl-beta-methylcholine representing 0.025% of the detected beta-methylcholine. Although pretreatment with hemicholinium-3 reduced the amount of acetylated product formed from either analog, the reduction was significant only for acetyl-beta-methylcholine (p less than 0.01).
D000107:Acetylation; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002794:Choline / Q000031:analogs & derivatives* / Q000378:metabolism; D002849:Chromatography, Gas / Q000379:methods; D006426:Hemicholinium 3 / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
C007954:homocholine; D006426:Hemicholinium 3; C044887:beta-methylcholine; D002794:Choline