Development of a new human breast cancer cell line Ia-270.
A new human breast cancer cell line (Ia-270) has been isolated from a malignant pleural effusion from a woman with metastatic infiltrating ductal carcinoma of the breast. This cell line contains cytoplasmic estrogen (ER) and progesterone (PR) receptors. Following estradiol (E2) administration, PR synthesis is augmented and a higher level of saturation density is reached. In an athymic mouse, the cell line produced a tumor morphologically similar to the primary tumor. The results of isoenzyme and karyotype analyses demonstrate Ia-270 to be of human origin and free of HeLa cell contamination. The cell line has been maintained in continuous culture since April 1982 and may provide a useful in vitro system for studying the biology of human breast cancer.
D000368:Aged; D000818:Animals; D001943:Breast Neoplasms / Q000032:analysis / Q000235:genetics / Q000473:pathology*; D002460:Cell Line; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000032:analysis; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D011960:Receptors, Estrogen / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis
D007527:Isoenzymes; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone