title: The cellular distribution of calcium in freeze-dried rabbit vas deferens using EM autoradiography.
abstract: The role of calcium in initiating smooth muscle contraction is widely accepted. The sources of this calcium are thought to be located both intracellularly and extracellularly. We have recently developed a method by which the cellular localization of calcium in smooth muscle can be determined. This method involves exposing the tissue to 45Ca, rapidly freezing and vacuum dehydrating the tissue, and preparing the tissue for electron microscopic autoradiography (EM ARG). In the present study the distribution of calcium in control and potassium-contracted tissue of the rabbit vas deferens was compared. No significant differences in distribution were observed in the two treatments. This finding provides morphological support for the hypothesis that the calcium used in potassium-induced contraction is primarily of extracellular origin. In addition, significant sequestration by intracellular organelles does not occur during a potassium contraction. In other experiments, the effect of rinsing tissue in cold calcium prior to freezing was investigated. From these data is appears that calcium is removed from the cytoplasmic matrix, plasma membrane, and organelles in a nonuniform manner. Further investigation into these findings should enable us to characterize more precisely the intracellular redistribution of calcium that occurs as a result of a variety of physiological manipulations.
mesh_terms: D000818:Animals; D001345:Autoradiography; D002118:Calcium / Q000378:metabolism*; D005612:Freeze Drying; D008297:Male; D008722:Methods; D008854:Microscopy, Electron; D009119:Muscle Contraction; D011817:Rabbits; D014018:Tissue Distribution; D014649:Vas Deferens / Q000166:cytology / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure
chemical_list: D002118:Calcium