Measurement of penicillamine and N-acetylcysteine in human blood by high-performance liquid chromatography and electrochemical detection.
A rapid and precise high-performance liquid chromatographic assay for both N-acetylcysteine and penicillamine in blood samples is described using selective reductive electrochemical detection and a high-efficiency C18 reversed-phase column. The use of an internal standard compensated for changes in detector responses during a run and for variable sample recovery. The detection limits for N-acetylcysteine and penicillamine were 25 and 10 ng/ml, respectively, using 500-microliters blood samples. Reproducibility of measurement for both thiols was excellent. This method allows routine monitoring of blood levels and pharmacokinetic studies with N-acetylcysteine and penicillamine.
D000111:Acetylcysteine / Q000097:blood*; D001172:Arthritis, Rheumatoid / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D004563:Electrochemistry; D006801:Humans; D007700:Kinetics; D010396:Penicillamine / Q000097:blood*; D013438:Sulfhydryl Compounds / Q000097:blood
D013438:Sulfhydryl Compounds; D010396:Penicillamine; D000111:Acetylcysteine