Studies on residual antibacterials in foods. III. High-performance liquid chromatographic determination of penicillin G in animal tissues using an on-line pre-column concentration and purification system.
A simple and reproducible method for the determination of residual penicillin G in edible animal tissues by high-performance liquid chromatography (HPLC) is described. The method consists in an off-line clean-up step using a basic aluminium oxide column and a Sep-Pak C18 cartridge and an on-line pre-column concentration and purification system. The procedure shows good sensitivity and precision. The recoveries from cattle liver, kidney and muscle fortified with 1 microgram/g of sodium penicillin G were 75.0-92.6% and the relative standard deviations were 2.35-4.06%. The detection limit corresponded to 0.05 micrograms/g of sodium penicillin G in animal tissues.
D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D005504:Food Analysis; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis; D009132:Muscles / Q000032:analysis; D010400:Penicillin G / Q000032:analysis*
D000890:Anti-Infective Agents; D010400:Penicillin G