title: Clotting factors secreted by monocytes and macrophages: analytical considerations.
abstract: The secretion of clotting factors by rat spleen macrophages and human peripheral blood monocytes has been studied. The results show that the amount of clotting factors measured depends critically upon the characteristics of the assay system used. The presence of warfarin, salicylic acid or thrombin in the culture medium is shown to decrease the vitamin K dependent clotting factor activity in the supernatant after in vitro culture of rat spleen macrophages and human peripheral blood monocytes.
mesh_terms: D000818:Animals; D001779:Blood Coagulation Factors / Q000032:analysis*; D002478:Cells, Cultured; D006801:Humans; D008264:Macrophages / Q000032:analysis* / Q000378:metabolism; D009000:Monocytes / Q000032:analysis* / Q000378:metabolism; D051381:Rats; D013917:Thrombin / Q000494:pharmacology; D010738:Type C Phospholipases / Q000494:pharmacology
chemical_list: D001779:Blood Coagulation Factors; D010738:Type C Phospholipases; D013917:Thrombin