Non-specific binding of mouse IgM antibodies to lipid antigens.
Galactocerebroside (GC) is a major component expressed on the surface of oligodendrocytes and Schwann cells. It plays a role in neuronal-glial interaction that results in myelinization. In this paper we describe a method to estimate anti-GC antibodies by ELISA. Furthermore we found that IgM antibodies of mice immunized with GC-unrelated antigens, and which are not specific for GC, bind in a non-specific way to this compound. Thus only assays which avoid the detection of IgM antibody can be used to estimate anti-GC antibodies. Moreover, we showed that serum of immunized mice bind also non-specifically to brain cells of newborn mice.
D000818:Animals; D000941:Antigens / Q000276:immunology*; D001921:Brain / Q000166:cytology / Q000276:immunology; D002554:Cerebrosides / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005620:Freund's Adjuvant / Q000378:metabolism; D005699:Galactosylceramides / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000941:Antigens; D002554:Cerebrosides; D005699:Galactosylceramides; D007075:Immunoglobulin M; D005620:Freund's Adjuvant