Anti-I-J alloantisera elicited by immunization of B10.A(3R) (I-Jb) mice with bone marrow-derived macrophages from B10.A(5R) (I-Jk) mice.
In this paper we describe production of alloantisera specific for determinants encoded by I-J gene loci expressed on macrophages. B10.A(3R) (I-Jb) mice were hyperimmunized with pure macrophages grown in vitro from bone marrow stem cells of congenic B10.A(5R) mice. The antisera contained predominantly IgM antibody that was non-adherent to protein-A-Sepharose with a minor component of IgG1, and IgG2a antibodies that were adherent to protein-A-Sepharose. The protein-A non-adherent antibody completely blocked the in vitro generation of humoral immune responses to sheep erythrocytes by spleen cell from B10.A(5R) mice and from inbred strains that share the I-Jk haplotypes, but did not alter the responses of spleen cells of the I-Jb haplotype. In the presence of complement, both protein-A adherent and protein-A non-adherent antibodies eliminated the capacity of B10.A(5R) spleen cells to generate humoral and proliferative responses, but the functional activity of B10.A(3R) cells was unaffected. These data indicate the I-Jk specificity of the antisera. The capacity of the anti-macrophage antibody to block humoral immune induction was removed by absorption with bone marrow-derived macrophages from B10.A(5R) mice, but not from B10.A(3R) mice. Further, the B10.A(5R) macrophages completely restored the humoral responses of antibody- and complement-treated B10.A(5R) spleen cells, but B10.A(3R) macrophages showed only partial restoration that was consistent with a factor-mediated allogeneic effect. These data demonstrate the specificity of our anti-I-J sera for macrophages and indicate that bone marrow-derived macrophages express surface I-J encoded molecules.
D000818:Animals; D000918:Antibody Specificity; D001854:Bone Marrow Cells; D003602:Cytotoxicity, Immunologic; D006649:Histocompatibility Antigens / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology*; D007136:Immunoglobulins / Q000096:biosynthesis; D008264:Macrophages / Q000276:immunology* / Q000637:transplantation; D051379:Mice; D008815:Mice, Inbred Strains
D006649:Histocompatibility Antigens; D007106:Immune Sera; D007136:Immunoglobulins