Characteristics of the asparagine-linked oligosaccharide from honey-bee venom phospholipase A2. Evidence for the presence of terminal N-acetylglucosamine and fucose in an insect glycoprotein.
Eighty-eight % of phospholipase A2 from honey-bee (Apis mellifica) venom is glycosylated. Its single oligosaccharide exists in several structural variants, which represent consecutive stages of the "N-glycan processing pathway". The carbohydrate carries terminal fucosyl and N-acetyl-glucosaminyl residues. This is in contrast to earlier reports which suggest the lack of respective glycosyl-transferases in insects.
D000117:Acetylglucosamine / Q000032:analysis*; D000818:Animals; D001216:Asparagine; D001514:Bee Venoms / Q000032:analysis*; D001516:Bees / Q000201:enzymology; D002240:Carbohydrate Sequence; D005643:Fucose / Q000032:analysis*; D005944:Glucosamine / Q000031:analogs & derivatives*; D006020:Glycopeptides / Q000032:analysis; D006023:Glycoproteins; D008358:Mannose / Q000032:analysis; D008745:Methylation; D009844:Oligosaccharides / Q000032:analysis*; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2
D001514:Bee Venoms; D006020:Glycopeptides; D006023:Glycoproteins; D009844:Oligosaccharides; D005643:Fucose; D001216:Asparagine; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D005944:Glucosamine; D008358:Mannose; D000117:Acetylglucosamine