Demonstration of endogenous "imipramine like" material in rat brain.
The extraction and partial purification of an endogenous "imipramine- like" material from rat brain is described. The endogenous factor obtained after gel filtration and silica chromatography inhibits [3H] imipramine specific binding and mimics the inhibitory effect of imipramine on [3H] serotonin uptake in both brain and platelet preparations. The effects of the endogenous material are dose-dependent and it inhibits [3H] imipramine binding in a competitive fashion. The factor is unevenly distributed in the brain with high concentration in the hypothalamus and low concentration in the cerebellum.
D000818:Animals; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D001923:Brain Chemistry; D002850:Chromatography, Gel; D004305:Dose-Response Relationship, Drug; D007099:Imipramine / Q000032:analysis* / Q000494:pharmacology; D008297:Male; D051381:Rats; D012701:Serotonin / Q000378:metabolism; D014018:Tissue Distribution
D012701:Serotonin; D007099:Imipramine