Inhibition of phosphatidylcholine synthesis does not alter uptake of transferrin by LM fibroblasts.
The receptor-mediated endocytosis of diferric transferrin by LM fibroblasts has been examined to determine if de novo synthesis of phosphatidylcholine is required for this process. To test this possibility, LM cells were allowed to internalize [125I]transferrin in the presence or absence of exogenous choline. Under conditions in which de novo synthesis of phosphatidylcholine was reduced from 51% of the total to less than 10%, the initial rate of transferrin uptake was unaffected. These data suggest that the process of receptor-mediated endocytosis of transferrin can proceed normally in the absence of de novo phosphatidylcholine synthesis.
D000818:Animals; D002794:Choline / Q000502:physiology; D002855:Chromatography, Thin Layer / Q000379:methods; D003642:Deanol / Q000494:pharmacology; D004705:Endocytosis; D005347:Fibroblasts / Q000378:metabolism; D007700:Kinetics; D007739:L Cells; D008563:Membrane Lipids / Q000096:biosynthesis / Q000502:physiology*; D051379:Mice; D010713:Phosphatidylcholines / Q000096:biosynthesis / Q000502:physiology*; D011485:Protein Binding; D014168:Transferrin / Q000378:metabolism*
D008563:Membrane Lipids; D010713:Phosphatidylcholines; D014168:Transferrin; D003642:Deanol; D002794:Choline