Granulocyte concentrates. Glucose concentrations and glucose utilization during storage at 22 degrees C.
Neutrophil metabolism is dependent on the available stores of either exogenous or endogenous glucose. We studied 38 granulocyte concentrates collected by four different centrifugal cytapheresis systems: Haemonetics model 30, IBM 2997, Fenwal CS-3000, and Fenwal Celltrifuge II. Extracellular glucose concentrations were measured immediately following collection and after 24 hours of storage. The Fenwal CS-3000 produced the highest concentrations of platelets and leukocytes, and the units had the lowest initial glucose levels and pH. After 24 hours of storage, there was a mean glucose of 1.7 mg per dl in the Fenwal CS-3000 granulocyte concentrates and 9 of 12 units had no detectable glucose. In contrast, concentrates collected from other systems had sufficient glucose available to support neutrophil metabolism for a longer period.
D001786:Blood Glucose / Q000378:metabolism*; D001793:Blood Preservation; D002469:Cell Separation; D006098:Granulocytes / Q000378:metabolism* / Q000637:transplantation; D006801:Humans; D007937:Leukapheresis / Q000295:instrumentation; D007958:Leukocyte Count; D013696:Temperature; D013997:Time Factors
D001786:Blood Glucose