Cytoprotective effects of Bencianol on porcine vascular endothelial cells in vitro.
Endothelial cells of porcine thoracic aorta were chosen as in vitro model to study the reaction of endothelial cells to injury induced by carbon tetrachloride (CCl4 0.2 microliter/ml). Lactate dehydrogenase (LDH) was used as the biochemical marker of cell injury. The changes in the morphology of the surface endothelial cells were observed by scanning electron microscopy. In addition, cell survival rate after replating cultures was evaluated. The pretreatment with Bencianol (10(-10) M-10(-7) M) produced cytoprotective effects against CCl4 induced cell injury on the above three parameters.
D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects* / Q000648:ultrastructure; D002251:Carbon Tetrachloride / Q000494:pharmacology; D002470:Cell Survival; D002478:Cells, Cultured; D004727:Endothelium / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D005419:Flavonoids / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D013552:Swine
D005419:Flavonoids; C047756:bencianol; D002251:Carbon Tetrachloride; D007770:L-Lactate Dehydrogenase