Nerve growth factor. A structural relationship between its proteolytic and leukocyte-chemotactic active sites.
High molecular weight mouse nerve growth factor (HMW-NGF), in addition to its effects on certain neural elements, is also chemotactic for human polymorphonuclear leukocytes. One of the subunits of HMW-NGF is a protease of the serine family and its active site contains a serine residue and a closely-neighboring histidine residue that are both essential for proteolysis. Elimination of enzyme activity by irreversibly blocking the single serine has no effect on leukotaxis, but blocking the histidine abolishes leukotaxis. These results suggest the possibility that part of the proteolytic active site of this enzyme may have evolved to perform more than one, completely different, biologic function-proteolysis as well as nonproteolytically mediated chemotaxis.
D000818:Animals; D001665:Binding Sites; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D046911:Macromolecular Substances; D051379:Mice; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000032:analysis* / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects
D046911:Macromolecular Substances; D009414:Nerve Growth Factors