Distinction of G0 cells from senescent cells in cultures of non-cycling human fetal lung fibroblasts by anti-MAP-1 monoclonal antibody staining.
On staining with a monoclonal antibody raised against microtubule-associated protein-1 (MAP-1), dot-like structures were seen in the nuclei of interphase cells, but not in those of non-cycling G0-arrested cells. Dots were also not seen in the nuclei of non-cycling senescent human cells (IMR-90). A SV40-DNA-transformed subline of IMR-90 with a limited growth potential showed progressive decrease of cells with nuclei containing dots in the final stage of their lifespan. The dots appeared in G0-arrested IMR-90 cells when these cells were incubated in medium of high osmotic pressure for 3 min. In contrast, no dots appeared in senescent cells or X-ray-irradiated young cells when they were incubated in medium of high osmotic pressure. Thus irreversibly non-cycling cells could be distinguished from G0-phase cells on the level of whole cultures. The results suggest that senescent cells lose their division potential by entering an irreversible cell-cycle stage differing from G0.
D000911:Antibodies, Monoclonal; D002453:Cell Cycle; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure; D003470:Culture Media; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007399:Interphase; D007700:Kinetics; D008168:Lung / Q000166:cytology* / Q000196:embryology; D008869:Microtubule-Associated Proteins / Q000032:analysis*; D009994:Osmolar Concentration; D011247:Pregnancy
D000911:Antibodies, Monoclonal; D003470:Culture Media; D008869:Microtubule-Associated Proteins