A simple method to heparinize biological materials.
A new method was developed inducing antithrombogenicity to biologic materials. A basic protein, protamine, was used to increase heparin binding to collagenous materials. Protamine sulfate solution was poured into a collagen-rich material (porcine ureter). The protamine adsorbed to the collagen was then cross-linked to the material by glutaraldehyde. The collagen-protamine complex was then interacted with a heparin solution to ionically bind heparin to the material. The amount and the distribution of the bound heparin can be controlled by changing the protamine fixation to the material. A heparinized porcine ureter as a vascular substitute showed excellent antithrombogenicity in experimental animal studies. This method is expected to be used as method for developing antithrombogenic artificial organs, and as a method for the development of antiadhesive membranes.
D000818:Animals; D001665:Binding Sites; D001705:Bioprosthesis / Q000009:adverse effects; D001807:Blood Vessel Prosthesis / Q000009:adverse effects; D003094:Collagen; D004285:Dogs; D005260:Female; D006493:Heparin; D008297:Male; D008855:Microscopy, Electron, Scanning; D011479:Protamines; D011485:Protein Binding; D013552:Swine; D013927:Thrombosis / Q000517:prevention & control; D014513:Ureter
D011479:Protamines; D006493:Heparin; D003094:Collagen