New medium for solubilizing proteins of the eye lens core (nucleus).
The eye lens core (nucleus) is a practical source of proteins for use as genetic markers. A new, simple extraction solution for these proteins is described. The solution was tested on fish (mullet, rockfish, and barracuda) lens nuclei, which produced weak extracts with other extraction media. A new solution, in addition to producing the strongest extracts, demonstrated desirable qualities relating to electrophoresis and storage. This solution may serve as a general extraction solution for nuclear lens proteins.
D000818:Animals; D003459:Crystallins / Q000235:genetics / Q000302:isolation & purification*; D004586:Electrophoresis; D005399:Fishes; D007907:Lens Nucleus, Crystalline / Q000032:analysis*; D007908:Lens, Crystalline / Q000032:analysis*; D012995:Solubility
D003459:Crystallins