Segment alpha 182-198 of Torpedo californica acetylcholine receptor contains second toxin-binding region and binds anti-receptor antibodies.
The area around Cys-192 and Cys-193 is thought to be a functionally important part of the alpha-subunit of the acetylcholine receptor. We have synthesized peptide alpha 182-198 of the alpha-chain of the Torpedo californica acetylcholine receptor and investigated the binding to the peptide of alpha-bungarotoxin, cobratoxin and antibodies raised against acetylcholine receptor. The results showed that the synthetic peptide alpha 182-198 contains a second toxin-binding region and also binds a considerable fraction of anti-receptor antibodies. We also report here the toxin-binding activity of synthetic peptide alpha 125-148 of the human acetylcholine receptor which has been previously localized as a toxin-binding region in the alpha-chain of the Torpedo receptor.
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002038:Bungarotoxins / Q000378:metabolism; D004557:Electric Organ / Q000032:analysis; D010455:Peptides / Q000138:chemical synthesis; D011950:Receptors, Cholinergic / Q000032:analysis* / Q000276:immunology; D014101:Torpedo / Q000378:metabolism
D002038:Bungarotoxins; D010455:Peptides; D011950:Receptors, Cholinergic