Comparison of enzyme-linked immunosorbent assay, hemagglutination inhibition, and passive latex agglutination for determination of rubella immune status.
Hemagglutination inhibition, enzyme-linked immunosorbent assay (ELISA), and passive latex agglutination were used to test 495 human serum samples for determination of rubella immunity. Overall agreements for immune status were as follows: hemagglutination inhibition versus ELISA, 94.7% (469 of 495); hemagglutination inhibition versus passive latex agglutination (1:10 dilution), 99.2% (491 of 495); and passive latex agglutination (1:10 dilution) versus ELISA, 94.7% (469 of 495). Both ELISA and passive latex agglutination are satisfactory for rubella immunity screening, with the reservation that the ELISA method examined yielded a large number of false-negative results.
D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007841:Latex Fixation Tests; D012409:Rubella / Q000276:immunology*; D012412:Rubella virus / Q000276:immunology*
D000914:Antibodies, Viral