title: Morphological changes in the blastocyst of the western spotted skunk during activation from delayed implantation.
abstract: Blastocysts collected from the spotted skunk during delay of implantation, early activation and late activation demonstrate three-tiered growth and developmental changes. The slow-growing blastocyst from the several months of delay is small (less than 1.1 mm) with a rounded inner cell mass consisting of clusters of rounded, lipid-filled cells. During the several days of early activation, the lipid in both inner cell mass and trophoblast diminishes, polyribosomes increase in number, and the endodermal layer differentiates as the blastocyst grows (1.2-1.6 mm). At activation the inner cell mass flattens, becomes uncovered by polar trophoblast, and forms a disc of columnar epiblast cells. The blastocyst expands rapidly during the last 24-48 h prior to implantation to 1.7-2.0 mm, and the trophoblast becomes cuboidal with a marked endocytotic apparatus. The morphological evidence, together with previous studies of protein and RNA synthesis, suggests a tooling-up period during early activation with progressive increases in rates of growth and differentiation in the last hours as implantation approaches.
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology*; D002335:Carnivora / Q000502:physiology*; D010064:Embryo Implantation; D010065:Embryo Implantation, Delayed; D005260:Female; D012889:Mephitidae / Q000502:physiology*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008871:Microvilli / Q000648:ultrastructure; D011247:Pregnancy; D011270:Pregnancy, Animal; D014327:Trophoblasts / Q000166:cytology