title: Changes in the structure of somato-sensitive neurons in cat following retrograde uptake of HRP.
abstract: Neurons of cat cerebral cortex, area S1, labelled by retrograde transport of HRP, have been studied by electron microscopy; the animals were sacrificed 48 h after HRP injection. The cells studied are those in which the enzyme is found both in granular and in diffused form in the cytoplasm (diffuse-granular HRP neurons, DGN), or in diffused form in the cytoplasm (Golgi-like labelled neurons, GLN). The observations have shown that the certain recognition of DGN through electron microscopy, requires a comparison with a semithin section adjacent to the ultrathin section since the labelling granules are light-scattering in dark field. Instead the GLN are not light-scattering in dark field but are directly and unmistakably identifiable even by electron microscopy. The DGN are well preserved on the whole in all their organelles; the GLN show a highly damaged aspect in relation to both the Golgi apparatus and the smooth reticulum, to the cytoplasmic organelles and to the nucleus. Our hypothesis is that the latter neurons take on this appearance because of the combined action of: a) the peripheral mechanical lesions caused by the needle and b) the enormous amount of HRP that the cell is forced to take up (through these pathways) because of its lost integrity and because of the large quantity of marker around the axons. In conclusion intracellular HRP is identifiable by electron microscopy only when it is found in diffused form in the cytoplasm.
mesh_terms: D000818:Animals; D002415:Cats; D005260:Female; D006056:Golgi Apparatus / Q000648:ultrastructure; D006735:Horseradish Peroxidase / Q000378:metabolism*; D008297:Male; D008854:Microscopy, Electron; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure; D010544:Peroxidases / Q000378:metabolism*; D013003:Somatosensory Cortex / Q000378:metabolism* / Q000648:ultrastructure; D014018:Tissue Distribution
chemical_list: D006735:Horseradish Peroxidase; D010544:Peroxidases