Binding and activation of hemolytic complement by IgG antibodies: cooperativity between antibodies of different hapten specificity.
The ability of IgG antibodies with different hapten specificities to fix C1 and activate C as a function of hapten density on a red cell surface was investigated. Rabbit anti-methotrexate and anti-folinic acid IgG antibodies in a mixture were highly efficient in fixing C1 and activating C when cells carried simultaneously high levels of both haptens. We wished to find out whether in a C-activating IgG complex both IgG molecules had to be in a form that could activate C1. By reducing hapten density of one of the haptens on a double labeled cell, complexes were generated where only one in a pair of IgG molecules was in the activating form; such a pair had the same activating efficiency as a pair in which both IgG molecules were in the activating form. It was concluded that cooperative activation of C in C1-binding IgG complexes required only one IgG in the complex to be in the activating form.
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D003167:Complement Activation; D003172:Complement C1 / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D006168:Guinea Pigs; D006241:Haptens; D006461:Hemolysis; D007074:Immunoglobulin G / Q000276:immunology*; D012756:Sheep
D000906:Antibodies; D000936:Antigen-Antibody Complex; D003172:Complement C1; D006241:Haptens; D007074:Immunoglobulin G