Cloning of sequences induced and suppressed by administration of PSK, antitumor protein-bound polysaccharide.
To elucidate the effects of PSK, a protein-bound polysaccharide from Coriolus versicolor, on gene expression in tumor cells, we prepared cDNA clone libraries from PSK-treated and untreated cells of a rat ascites hepatoma line, AH66, which was previously shown to be susceptible to the antitumor action of this compound. Two PSK-induced and one suppressed cDNA clones were selected from these libraries by using a differential colony hybridization and RNA blot hybridization. PSK was thus shown to have a direct effect on the transcription and consequently on the translation of tumor cells.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001483:Base Sequence; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000378:metabolism; D005260:Female; D008114:Liver Neoplasms, Experimental / Q000378:metabolism; D009363:Neoplasm Proteins / Q000096:biosynthesis; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis / Q000187:drug effects*; D011509:Proteoglycans / Q000494:pharmacology*; D012334:RNA, Neoplasm / Q000378:metabolism; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects*
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D011509:Proteoglycans; D012334:RNA, Neoplasm; C010770:polysaccharide-K