Heterogeneity of cross-reactivity of mouse anti-benzylpenicilloyl antibodies against cephalothin haptens or polymers.
The cross-reactivity of mouse anti-benzylpenicilloyl antibodies against cephalothin-haptens and cephalothin-polymers was divergent depending on the source and isotype of antibodies and the methods to raise them. High cross-reactivity nearly equal to the reactivity to homologous haptens and polymers was obtained only with the IgE antibody of C57BL/6J mouse raised shortly after an immunizing injection of the emulsion containing Freund's complete adjuvant and benzylpenicilloyl-bovine gamma-globulin conjugate. With the other antibody preparations, cross-reactivity was moderate to negligible.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D002512:Cephalothin / Q000276:immunology*; D003429:Cross Reactions; D005260:Female; D006241:Haptens; D007074:Immunoglobulin G / Q000032:analysis; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D010400:Penicillin G / Q000031:analogs & derivatives* / Q000276:immunology; D011108:Polymers; D051381:Rats
D000906:Antibodies; D006241:Haptens; D007074:Immunoglobulin G; D011108:Polymers; C030990:benzylpenicilloic acid; D010400:Penicillin G; D002512:Cephalothin