DNA probe localization at 18p113 band by in situ hybridization and identification of a small supernumerary chromosome.
Recombinant plasmid clone B74 (also named D18 S3) containing a human single-copy DNA segment of 6 kilobases (kb) was localized by in situ hybridization on band p113 of chromosome 18. This probe was then used in cytogenetic diagnosis to identify precisely a small supernumerary chromosome as an isochromosome i(18p).
D001345:Autoradiography; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002902:Chromosomes, Human, 16-18; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D005260:Female; D006801:Humans; D008607:Intellectual Disability / Q000235:genetics; D007621:Karyotyping; D008214:Lymphocytes / Q000648:ultrastructure; D008297:Male; D009693:Nucleic Acid Hybridization; D014314:Trisomy
D004247:DNA