title: Immunogenicity, immunological cross reactivity and non-specific irritant properties of the exudate gums, arabic, karaya and tragacanth.
abstract: An animal model has been used to investigate the immunogenicity and non-specific irritant properties of exudate gums. The materials studied were four preparations of gum arabic (Acacia spp.), two of gum karaya (Sterculia spp.), two of gum tragacanth (Astralagus spp.) and a residue obtained after ethanol extraction of gum arabic. Groups of animals were intradermally immunized with the gum in complete Freund's adjuvant. Serum antibody levels were measured by an ELISA technique and delayed hypersensitivity responses by a footpad swelling test. Antigenic cross-reactivity within each gum species was tested in a crossover fashion. All gum preparations elicited systemic immune responses after immunization. Further processing reduced immunogenicity, although there was no evidence that systemic immunity to these complex polysaccharide antigens responses could be completely abolished by processing or purification. The ethanolic extract, and some of the gum preparations, particularly tragacanth and karaya, caused considerable footpad swelling when injected intradermally. It is concluded that processing and awareness of subspecies differences can reduce the inherent immunogenicity and potential irritant effects of exudate gums.
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D003429:Cross Reactions; D004487:Edema / Q000209:etiology; D004797:Enzyme-Linked Immunosorbent Assay; D006170:Gum Arabic / Q000276:immunology* / Q000633:toxicity; D007114:Immunization; D007509:Irritants; D007618:Karaya Gum / Q000276:immunology* / Q000633:toxicity; D051379:Mice; D011134:Polysaccharides / Q000276:immunology*; D014144:Tragacanth / Q000276:immunology* / Q000633:toxicity
chemical_list: D007509:Irritants; D011134:Polysaccharides; D006170:Gum Arabic; D007618:Karaya Gum; D014144:Tragacanth