title: Heparin inhibition of ferredoxin-NADP reductase in chloroplast thylakoid membranes.
abstract: Heparin, an anionic polysaccharide, inhibited the ferredoxin-catalyzed reduction of NADP in spinach chloroplast thylakoid membranes. Under the same conditions of assay, heparin did not interfere markedly with photoreduction of methyl viologen, anthraquinone sulfonate, or ferredoxin. A kinetic analysis of the heparin-induced interference with NADP photoreduction showed partial competitive inhibition. Heparin also interfered with NADPH oxidation by membrane-bound ferredoxin-NADP reductase (with dichlorophenol-indophenol as the acceptor) by a mechanism that involves partial competitive inhibition. This reaction was sensitive to the presence of salts; increasing ionic strength increases the heparin Ki for inhibition of NADPH oxidation. These results show that heparin binds to ferredoxin-NADP reductase, and in doing so interferes with binding to the reductase by both ferredoxin and NADP(H). Since heparin is redox inactive and does not interfere with the photophosphorylation reaction, it is a useful inhibitor of thylakoid membrane reactions which require the catalytic activity of ferredoxin-NADP reductase.
mesh_terms: D002384:Catalysis; D002736:Chloroplasts / Q000201:enzymology*; D004579:Electron Transport / Q000187:drug effects; D005287:Ferredoxin-NADP Reductase / Q000037:antagonists & inhibitors*; D005288:Ferredoxins / Q000378:metabolism; D006493:Heparin / Q000494:pharmacology*; D007425:Intracellular Membranes / Q000201:enzymology; D007700:Kinetics; D009247:NADH, NADPH Oxidoreductases / Q000037:antagonists & inhibitors*; D009249:NADP / Q000378:metabolism; D010944:Plants; D011485:Protein Binding / Q000187:drug effects
chemical_list: D005288:Ferredoxins; D009249:NADP; D006493:Heparin; D005287:Ferredoxin-NADP Reductase; D009247:NADH, NADPH Oxidoreductases