title: Distribution pattern of drained antigens and antibodies in the subcapsular sinus of the lymph node of the rat.
abstract: A recent study of lymph nodes of the rat showed that they are morphologically and physiologically compartmentalized. A compartment of a node includes a portion of subcapsular sinus into which the lymph, entering via the related afferent lymphatic opening, is filtered. The study also showed that colloidal carbon injected locally in a small dose becomes predominantly associated with the areas of the inner wall of the subcapsular sinus that cover the extra-follicular zone of the peripheral cortex. Little carbon is seen over the folliculo-nodules (follicles with a nodule or germinal center). The question arose as to whether drained natural substances, as antigens and antibodies, follow the same pattern of distribution in the subcapsular sinus as the carbon. Therefore, small doses of fluorescein isothyocyanate (FITC)-conjugated antigens were injected locally into normal rats whose own antibodies in the nodes were stained by immunofluorescence. The pattern of distribution of the antigens in the draining nodes was found to be the same as that of the carbon. Furthermore, the lymph-carried antibodies of the rats were found to follow the same pattern. The morphological basis for such a pattern is explained. The results are further discussed with regard to the probable normal entry route of lymph-carried antigens in the parenchyma of nodes.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000941:Antigens / Q000302:isolation & purification*; D007120:Immunochemistry; D008198:Lymph Nodes / Q000033:anatomy & histology / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000906:Antibodies; D000941:Antigens