Rat coagulation factor V purification and production of the monospecific antiserum.
An original method was applied to purify rat factor V. The final preparation had a sp. act. of 45 U/ml for a 2500-fold purification with a yield of 43%. The final product is partially activated since it is 4.7-fold activable by RVV-VAE vs 6.3 in plasma. It can explain the presence of some of the four slightly stained additional bands found in SDS-electrophoresis. Finally, results of the purification suggest that rat factor V is a 338,000 single chain glycoprotein with a strong molecular asymmetry. A factor V deficient fraction was produced and used to adsorb an anti-factor V antiserum. This adsorbed antiserum was found monospecific against purified rat factor V.
D000818:Animals; D001777:Blood Coagulation; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D005165:Factor V / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D007106:Immune Sera; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G; D051381:Rats; D011919:Rats, Inbred Strains
D007106:Immune Sera; D007074:Immunoglobulin G; D005165:Factor V