Validation of techniques for the destruction of dimethyl sulfate.
It has been reported that dimethyl sulfate (DMS) can be degraded with sodium hydroxide solution (1 mol/L), sodium carbonate solution (1 mol/L), or ammonium hydroxide solution (1.5 mol/L). This has now been confirmed. Complete destruction of undiluted DMS or DMS in solvents miscible with water (methanol, ethanol, DMSO, DMF, acetone) or solvents partially miscible or immiscible with water (toluene, p-xylene, benzene, 1-pentanol, ethyl acetate, chloroform, carbon tetrachloride, acetonitrile) could be obtained using any of the above methods. Reaction times were 15 min after homogeneity was obtained for undiluted DMS, 15 min for solutions in methanol, ethanol, DMSO, and DMF, one hour for solutions in acetone, three hours for acetonitrile, and one day for the other solvents listed above. The final reaction mixtures were tested for mutagenicity, and when the solutions were not cytotoxic, no mutagenic response was obtained. DMS in solution was determined by a colorimetric method. The products of the reactions were found to be methanol when NaOH and Na2CO3 were used and methylamine, dimethylamine, trimethylamine, and methanol when ammonium hydroxide was used. The stability of DMS in various solvents was also determined.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D004355:Drug Stability; D066298:In Vitro Techniques; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis*; D051381:Rats; D012997:Solvents; D013463:Sulfuric Acid Esters / Q000032:analysis* / Q000633:toxicity; D013464:Sulfuric Acids / Q000032:analysis*
D009153:Mutagens; D012997:Solvents; D013463:Sulfuric Acid Esters; D013464:Sulfuric Acids; C007482:dimethyl sulfate