S-100 proteins and microtubules: analysis of the effects of rat brain S-100 (S-100b) and ox brain S-100a0, S-100a and S-100b on microtubule assembly-disassembly.
Rat brain S-100 (S-100b) and ox brain S-100a0, S-100a and S-100b have been tested for their ability to control the assembly and disassembly of brain microtubule proteins in the presence of either Ca2+ or Zn2+, in vitro. In the presence of Ca2+, single S-100 isoforms have similar, if not identical, effects, i.e. they inhibit assembly and promote disassembly. In the presence of Zn2+ from 0.1 to 1 mM (free concentration), rat S-100 and ox S-100a and S-100b inhibit assembly, while S-100a0 is without effect. These data are briefly discussed in relation to the cellular localization of single S-100 isoforms in the brain.
D000818:Animals; D015415:Biomarkers; D002118:Calcium / Q000494:pharmacology; D002417:Cattle; D004357:Drug Synergism; D007700:Kinetics; D046911:Macromolecular Substances; D008870:Microtubules / Q000187:drug effects* / Q000502:physiology; D009414:Nerve Growth Factors; D011487:Protein Conformation / Q000187:drug effects; D051381:Rats; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000494:pharmacology*; D015032:Zinc / Q000494:pharmacology
D015415:Biomarkers; D046911:Macromolecular Substances; D009414:Nerve Growth Factors; C030564:S-100 calcium-binding protein alpha subunit; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D015032:Zinc; D002118:Calcium