Plasma membrane isolation and fatty acid analysis of membrane lipids from murine lymphocytes.
Plasma membranes have been isolated in high yield by a novel method from splenic lymphocytes of BALB/c mice. These membranes were purified in predominantly envelope form, essentially right-side out, by zinc stabilization and sucrose density gradient centrifugation. A simple, rapid method was used for derivatization and analysis of fatty acids in plasma membrane lipids.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000032:analysis / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000097:blood*; D005260:Female; D008214:Lymphocytes / Q000032:analysis* / Q000648:ultrastructure; D008563:Membrane Lipids / Q000097:blood*; D051379:Mice; D008807:Mice, Inbred BALB C
D005227:Fatty Acids; D008563:Membrane Lipids