Activation of the alternative complement pathway by intraocular lenses.
To determine if posterior chamber polymethylmethacrylate lenses with polypropylene loops activate complement, the authors measured levels of C3a, C4a and C5a by radioimmunoassay in human sera incubated with and without these lenses. Human sera incubated with intraocular lenses showed elevated levels of C3a and C5a but no change in C4a. There were no statistically significant differences in the generation of activated complement by polypropylene loops vs polymethylmethacrylate optics. The authors also compared the ability of intraocular lenses to activate complement with that of zymosan and endotoxin, known activators of the alternative pathway. Our results suggest that polymethylmethacrylate lenses with polypropylene loops generate C3a and C5a by activation of the alternative complement pathway.
D000328:Adult; D003167:Complement Activation / Q000187:drug effects; D003176:Complement C3 / Q000032:analysis; D003181:Complement C4 / Q000032:analysis; D003182:Complement C5 / Q000032:analysis; D003170:Complement Pathway, Alternative / Q000187:drug effects; D004731:Endotoxins / Q000494:pharmacology; D006801:Humans; D007910:Lenses, Intraocular / Q000009:adverse effects*; D008768:Methylmethacrylates / Q000009:adverse effects*; D011126:Polypropylenes / Q000009:adverse effects; D011863:Radioimmunoassay; D014605:Uveitis / Q000209:etiology / Q000276:immunology; D015054:Zymosan / Q000494:pharmacology
D003176:Complement C3; D003181:Complement C4; D003182:Complement C5; D004731:Endotoxins; D008768:Methylmethacrylates; D011126:Polypropylenes; D015054:Zymosan