Prestalk/prespore differentiation tendency of Dictyostelium discoideum cells as detected by a stalk-specific monoclonal antibody.
By the use of a prestalk- and stalk-specific monoclonal antibody, production of prestalk antigen was examined with non-glucose grown [G(-)] and glucose grown [G(+)] cells of Dictyostelium discoideum AX2. Unlike wild type (NC4), some growth phase cells of AX2 were reactive with the antibody. However, G(-) cells contained much more antigen than G(+) cells and the difference between the two remained during the preaggregation period. Besides glucose, the addition of metabolizable, but not nonmetabolizable sugars to both growth phase and preaggregation cells suppressed the production of the prestalk antigen on the one hand and stimulated the accumulation of glycogen on the other hand. When mixed, G(-) cells which produced more prestalk antigen during the preaggregation period remained prestalk cells after aggregation, while G(+) cells which produced less antigen were converted to prespore cells. G(+) cells collected at the stationary phase [G(+)st] were stronger in prestalk sorting tendency than G(+) cells but weaker than G(-) cells. The prestalk antigen content of G(+)st cells prior to aggregation was an intermediate between those of G(-) and G(+) cells. These lead to the conclusion that the prestalk antigen content of preaggregation cells reflect the tendency of the cells toward either prestalk or prespore differentiation after aggregation.
D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis / Q000276:immunology; D002241:Carbohydrates / Q000494:pharmacology; D002454:Cell Differentiation; D002469:Cell Separation; D004023:Dictyostelium / Q000166:cytology* / Q000276:immunology; D005434:Flow Cytometry; D005947:Glucose / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D000941:Antigens; D002241:Carbohydrates; D005947:Glucose