In vitro synthesis of the gene coding for the glycoprotein E1 of Sindbis virus.
Ds cDNA of the 42S virionic RNA of Sindbis Virus has been synthesized and cloned in the plasmid pBR 322. Restriction map analysis and hybridization studies show that two clones cover the 3' end non coding region of the RNA, the whole membrane glycoprotein E1 and a peptide of MW 6,000 daltons. The use of these clones in experiments of gene expression in mammalian cell is discussed.
D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis*; D006023:Glycoproteins / Q000235:genetics*; D010957:Plasmids; D012845:Sindbis Virus / Q000235:genetics*
D004279:DNA, Viral; D006023:Glycoproteins