In vitro synthesis of superoxide dismutases of rat liver.
The syntheses of copper, zinc-superoxide dismutase (Cu,Zn-SOD) and manganese-superoxide dismutase (Mn-SOD) in vitro were studied. Both Cu,Zn-SOD and Mn-SOD were preferentially synthesized by free polysomes. Mn-SOD was synthesized as a large precursor (26,000 daltons), which was processed to the mature size (22,500 daltons) by in vitro incubation with a rat liver mitochondrial fraction. On the other hand, Cu,Zn-SOD was synthesized as the mature size product. It was shown that Cu,Zn-SOD and Mn-SOD synthesized in vitro represented 0.018% and 0.016% of the total translation products of free polysomes, respectively.
D000818:Animals; D001692:Biological Transport; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000201:enzymology*; D008345:Manganese / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology; D013482:Superoxide Dismutase / Q000096:biosynthesis* / Q000276:immunology
D008345:Manganese; D008715:Methionine; D013482:Superoxide Dismutase