Morphological and ultrastructural study of megakaryocytopoiesis in liquid cultures of hamster spleen.
A long-term liquid culture system of hemopoietic tissue derived from adult hamster spleen has been described. These primary liquid cultures can maintain stem cell proliferation and differentiation for more than three months without secondary repopulation. A characteristic of the liquid cultures is the formation of clusters of hemopoietic cells around adherent stromal cells. Some islands were composed exclusively of megakaryocytes and adherent cells. Isolation of these clusters of differentiating megakaryocytes and their adherent cellular substrate permitted the analysis of the morphological and ultrastructural features of the interaction between cells of megakaryocytic lineage with the adherent stroma.
D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D006224:Cricetinae; D046508:Culture Techniques; D005260:Female; D006410:Hematopoiesis; D008533:Megakaryocytes / Q000166:cytology / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning; D013154:Spleen / Q000166:cytology*