Localization of glucose-6-phosphate dehydrogenase in mouse and man by in situ hybridization: evidence for a single locus and transposition of homologous X-linked genes.
By hybridizing a tritiated human genomic probe (pGD3) to metaphase chromosomes in situ, we have localized the gene for glucose-6-phosphate dehydrogenase (G6PD) in both the human and mouse complement. The locus on the intact human X chromosome is close to the telomere on the long arm, confirming the assignment based on studies of an X/autosome translocation in human-mouse hybrids. Although the signal:background ratio was reduced for the heterologous hybridization of the human probe to mouse metaphases, 20% of the grains were on the X chromosome and 93% of these were in the A region, relatively close to the centromere. The location of G6PD in mouse and man reflects intrachromosomal transposition of these homologous X loci. Genomic DNAs from mouse and man and from hybrids with human X/autosome translocations were digested with several restriction enzymes including EcoRI, PstI, and HpaII, and Southern blots were probed with 32P-pGD3. The results of the analysis also confirm the human G6PD assignment and are consistent with a single copy of the locus in the haploid genome of both species.
D000818:Animals; D002478:Cells, Cultured; D002871:Chromosome Banding; D002874:Chromosome Mapping; D005260:Female; D005796:Genes; D008040:Genetic Linkage; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics*; D006801:Humans; D008214:Lymphocytes / Q000166:cytology; D008677:Metaphase; D051379:Mice; D009693:Nucleic Acid Hybridization; D013045:Species Specificity; D014960:X Chromosome
D005954:Glucosephosphate Dehydrogenase