The effect of Alcide, a new antimicrobial drug, on rat blood glutathione and erythrocyte osmotic fragility, in vitro.
Alcide is an antimicrobial drug which has been demonstrated to kill a variety of common pathogenic bacteria as well as fungi, in vitro. This agent is supplied in liquid and gel forms and consists of two parts, one of which contains sodium chlorite, while the other contains lactic acid as the active ingredients. Mixing of the two parts prior to use produces chlorine dioxide (ClO2), a strong oxidizing agent. A dose-dependent decrease in glutathione content and erythrocyte osmotic fragility occurred after incubation of whole blood with Alcide. Glutathione concentration and erythrocyte osmotic fragility approached the control values after 240 min of incubation with Alcide containing 1 mM NaClO2. The addition of exogenous glutathione (50 mg 100 ml-1) or glutathione reductase and NADPH to rat blood in the presence of Alcide returned erythrocyte osmotic fragility to control values. Treatment of rat blood with Alcide did not change glutathione reductase or glutathione peroxidase activities after 1 h of incubation.
D000818:Animals; D002713:Chlorine / Q000633:toxicity*; D017606:Chlorine Compounds; D004202:Disinfectants / Q000633:toxicity*; D005978:Glutathione / Q000097:blood*; D005979:Glutathione Peroxidase / Q000097:blood; D005980:Glutathione Reductase / Q000097:blood; D066298:In Vitro Techniques; D008297:Male; D009249:NADP / Q000378:metabolism; D009996:Osmotic Fragility / Q000187:drug effects; D010087:Oxides / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D017606:Chlorine Compounds; D004202:Disinfectants; D010087:Oxides; D002713:Chlorine; D009249:NADP; C025109:chlorine dioxide; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005978:Glutathione