Microchemical synthesis of the serotonin receptor ligand, 125I-LSD.
The synthesis and properties of 2-[125I]-lysergic acid diethylamide, the first 125I-labeled serotonin receptor ligand, are described. A novel microsynthesis apparatus was developed for this synthesis. The apparatus employs a micromanipulator and glass micro tools to handle microliter to nanoliter volumes on a microscope stage. This apparatus should be generally useful for the synthesis of radioligands and other compounds when limited amounts of material must be handled in small volumes.
D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism; D002855:Chromatography, Thin Layer; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods; D008238:Lysergic Acid Diethylamide / Q000138:chemical synthesis*; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods; D051381:Rats; D011985:Receptors, Serotonin / Q000378:metabolism*
D007457:Iodine Radioisotopes; D011985:Receptors, Serotonin; D008238:Lysergic Acid Diethylamide