Lectin blotting of human muscle. Identification of a high molecular weight glycoprotein which is absent or altered in Duchenne muscular dystrophy.
Using a combination of sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting, a high molecular weight Ricinus communis I (RCA I)-binding glycoprotein (approx. Mr 370000) has been identified in human muscle that is consistently altered or absent in muscle from patients with Duchenne muscular dystrophy (DMD). In addition, a Mr 54000 RCA I-binding glycoprotein was identified in 4 out and 8 DMD muscle samples that was not present in normal muscle. The possibility that the Mr 370000 glycoprotein could be a muscle membrane glycoprotein which is altered or absent in DMD is discussed.
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D007223:Infant; D008565:Membrane Proteins / Q000032:analysis; D008970:Molecular Weight; D009132:Muscles / Q000032:analysis*; D009136:Muscular Dystrophies / Q000235:genetics / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate; D013577:Syndrome
D006023:Glycoproteins; D008565:Membrane Proteins; D012967:Sodium Dodecyl Sulfate