Induction of sister-chromatid exchanges by N-nitrosocimetidine in cultured human lymphocytes and its inhibition by chemical compounds.
The effect of nitrosocimetidine (NC) on the frequency of sister-chromatid exchanges (SCEs) in human lymphocytes has been studied. The frequency of SCEs induced by a 1-h exposure to 2.6 X 10(-4) M NC was 4-fold greater than that in the solvent control. A 72-h exposure to NC had a similar dose-related effect. We also examined the effect of the sulfhydryl compounds cysteine, cysteamine, cystamine and glutathione, the reducing agent dithionite, and vitamins C and E on the NC-induced SCEs. None of these compounds induced SCEs. Cysteine, cysteamine, and cystamine significantly reduced the number of NC-induced SCEs, and the others did not.
D001205:Ascorbic Acid / Q000494:pharmacology; D002478:Cells, Cultured; D002927:Cimetidine / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000633:toxicity*; D003538:Cystamine / Q000494:pharmacology; D003543:Cysteamine / Q000494:pharmacology; D003545:Cysteine / Q000494:pharmacology; D004227:Dithionite / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008769:Methylnitronitrosoguanidine / Q000633:toxicity; D009603:Nitroso Compounds / Q000037:antagonists & inhibitors / Q000633:toxicity*; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D014810:Vitamin E / Q000494:pharmacology
D009603:Nitroso Compounds; D008769:Methylnitronitrosoguanidine; D014810:Vitamin E; D004227:Dithionite; D003543:Cysteamine; C019503:nitrosocimetidine; D002927:Cimetidine; D005978:Glutathione; D003545:Cysteine; D001205:Ascorbic Acid; D003538:Cystamine