Further studies on the extrusion of cytosol macromolecules by cultured chick embryo fibroblast cells.
The RNA and lipid-associated macromolecules extruded by chick embryo fibroblast cells have been analysed by chromatography. The results taken with those previously obtained suggest that cultured chick embryo fibroblasts excrete a wide range of cytosol macromolecules into the surrounding medium. As previously found with DNA and protein, the chromatographic patterns found with supernatant and cell cytosol preparations were closely similar, suggesting that the media supernatant macromolecules are cytosal derived. A proportion of the RNA and lipid associated material elutes at the same position as the previously observed for DNA and protein on gel exclusion chromatography, suggesting that cell cytosol contains a discrete DNA-RNA-protein lipid complex (size approx 5 X 10(5) dalton) which is extruded by cultured cells.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003470:Culture Media; D003600:Cytosol / Q000648:ultrastructure; D004247:DNA / Q000032:analysis*; D005347:Fibroblasts / Q000166:cytology*; D008055:Lipids / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D014316:Tritium
D003470:Culture Media; D008055:Lipids; D011506:Proteins; D014316:Tritium; D004247:DNA