Extracellular distribution of radiolabel obscures specific binding of diethylstilbestrol in mouse skeletal muscle.
The extracellular distribution of 3H-diethylstilbestrol (3H-DES) in mouse skeletal muscle was assessed following intraperitoneal injection. Total muscle extracellular space was measured with 14C-inulin, and the vascular space with 125I-albumin. A significant difference in the distribution of native 3H-DES and its metabolites in muscle and blood was found. This could only be explained if these compounds distributed with the albumin space and not the inulin space.
D000418:Albumins / Q000378:metabolism; D000818:Animals; D004054:Diethylstilbestrol / Q000097:blood / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism*; D007444:Inulin / Q000378:metabolism; D008297:Male; D051379:Mice; D009132:Muscles / Q000378:metabolism*; D011485:Protein Binding; D014316:Tritium
D000418:Albumins; D014316:Tritium; D004054:Diethylstilbestrol; D007444:Inulin