Enzyme-linked immunosorbent assay of black pigment dispersing hormone from the fiddler crab, Uca pugilator.
An enzyme-linked immunosorbent assay was developed for black pigment dispersing hormone (BPDH) obtained from the fiddler crab, Uca pugilator. Immunoreactive BPDH, as high as 8.8 +/- 0.9 ng/50 microliters, was found in the hemolymph of crabs with dispersed black pigment. High potassium saline caused 4.5 +/- 0.4 ng immunoreactive BPDH to be released into the medium from isolated crab eyestalks in 5 min.
D000818:Animals; D003386:Brachyura / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006458:Hemolymph / Q000032:analysis; D007447:Invertebrate Hormones / Q000032:analysis*; D011817:Rabbits; D013053:Spectrophotometry
D007447:Invertebrate Hormones; C005973:black pigment dispersing hormone