Correlation between immunosuppressive activity and translation regulatory activity.
An immunoglobulin negative material from the eluate of an anti-idiotype immunosorbent column [1] exhibited potent immunosuppressive activity. This material also inhibited the translation of globin mRNA in a cell-free reticulocyte lysate system. The translation inhibitory activity of this material was not attributable to nucleases which were separable by a blue-dextran agarose column. Further correlation between immunosuppressive activity and translation inhibitory activity was observed when GTP or GTP analogue was included in experimental systems. These results suggest that the immunosuppressive factor (or factors) may contain a translation inhibitory factor. The biochemical mechanism of immunosuppression is discussed.
D000818:Animals; D005914:Globins / Q000096:biosynthesis; D006160:Guanosine Triphosphate / Q000378:metabolism; D007108:Immune Tolerance; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D007166:Immunosuppressive Agents / Q000302:isolation & purification*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism
D007130:Immunoglobulin Idiotypes; D007166:Immunosuppressive Agents; D012333:RNA, Messenger; D006160:Guanosine Triphosphate; D005914:Globins