Sulfated glycosaminoglycan deposition and processing at the basal epithelial surface in branching and beta-D-xyloside-inhibited embryonic salivary glands.
We investigated whether the inhibition of proteoglycan synthesis and salivary branching morphogenesis by beta-D-xyloside was related to the deposition and processing of newly synthesized glycosaminoglycans at the basal epithelial surface that correlates with normal branching activity. Forty eight-hour cultures of control and 0.5 mM beta-xyloside-treated submandibular rudiments were labeled for 2 hr with [35S]sulfate and fixed and processed for autoradiography, immediately or after 2, 4, 6, or 8 hr of postlabeling chase in nonradioactive medium. The data demonstrated that deposition of chondroitin sulfate-rich material at the basal epithelial surface was strikingly reduced in beta-xyloside-treated rudiments, while patterns of label loss during postlabeling chase were not altered.
D000818:Animals; D001345:Autoradiography; D004848:Epithelium / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism*; D006027:Glycosides / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D012469:Salivary Glands / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism; D013462:Sulfur Radioisotopes
D006025:Glycosaminoglycans; D006027:Glycosides; D013431:Sulfates; D013462:Sulfur Radioisotopes; C010807:xylosides; C013786:A73025