Quantitative localization of polystyrene microspheres following microinjection in the avian metencephalic neural crest pathway.
Polystyrene microspheres were microinjected into crest populations at two preotic sites in Hamburger-Hamilton stage 10 and 11 chick embryos to investigate factors modulating cephalic neural crest fate. Analyses of microsphere localization and comparisons with cephalic crest fate maps indicated the following: microspheres injected at stage 10 localized with derivatives reflecting the fate of the crest population at the injection site; microspheres injected at stage 11 exhibited minimal displacement; and localization in ectoderm was similar in embryos injected at either stage. These results suggest that microinjected microspheres can be used to investigate normal and abnormal craniofacial morphogenesis.
D000818:Animals; D001921:Brain / Q000196:embryology*; D002465:Cell Movement; D002642:Chick Embryo / Q000254:growth & development*; D005109:Extracellular Matrix / Q000502:physiology; D008863:Microspheres; D009024:Morphogenesis; D009432:Neural Crest / Q000166:cytology*; D011137:Polystyrenes
D011137:Polystyrenes