Hepatic clearance of rat liver aspartate aminotransferase isozymes: evidence for endocytotic uptake via different binding sites on sinusoidal liver cells.
Rat liver aspartate aminotransferase (AAT) (EC 2.6.1.1) exists in two isozymic forms, cytosolic (c-AAT) and mitochondrial (m-AAT). The previous study (Kamimoto, Y. et al., Hepatology an accompanying paper in this issue) demonstrated that these isozymes were cleared from blood at different half-lives via adsorptive endocytosis by sinusoidal liver cells. To understand the cellular mechanism for the differential uptake of the isozymes, we have further studied in vivo uptake of 125I-labeled AAT isozymes by sinusoidal cells. The results indicated that the uptake of the isozymes occurred via a typical endocytotic pathway: the initial binding, internalization and subsequent degradation in the lysosomes. Quantitation of the isozymes bound to the cell surface prior to internalization either by binding at 4 degrees C or by a combined use of anti-AAT antibody and 125I-protein A at 37 degrees C revealed that the differential plasma clearance of AAT isozymes could be attributable to the isozymic difference in capacity of surface membranes to bind the isozymes. The uptake of 125I-c-AAT was inhibited by unlabeled c-AAT more significantly than by m-AAT, but not by other ligands known to be taken up by sinusoidal cells via receptor-mediated pathways. Similarly, the uptake of 125I-m-AAT was inhibited predominantly by unlabeled m-AAT. Similar ligand specificity was also demonstrated in the binding study at 4 degrees C. The binding of 125I-m-AAT and c-AAT followed saturation kinetics with an apparent Kd of 1.3 X 10(-6) M and 1.7 X 10(-6) M, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism*; D001665:Binding Sites; D003600:Cytosol / Q000201:enzymology; D004705:Endocytosis; D007527:Isoenzymes / Q000378:metabolism*; D008024:Ligands; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains
D007527:Isoenzymes; D008024:Ligands; D001219:Aspartate Aminotransferases