Demonstration of cathepsin B in the rat kidney using fluorescence histochemistry.
The procedure of mounting freeze-dried sections with celloidin was adapted for the fluorescent-histochemical demonstration of cathepsin B in the rat kidney. A good localization of reaction products was shown in freeze-dried, 5-micron sections which had been mounted free floating with 1.5% celloidin solution on albuminized slides. Using this procedure, the reaction products were localized in the lysosomes, particularly those of the convoluted proximal tubule.
D000818:Animals; D002401:Cathepsin B; D002403:Cathepsins / Q000378:metabolism*; D005453:Fluorescence; D006651:Histocytochemistry; D007668:Kidney / Q000201:enzymology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D002403:Cathepsins; D002401:Cathepsin B