[Surface charge of mouse neuroblastoma cells and changes during growth and morphologic differentiation of the cell population].
Microelectrophoresis method was used to study the surface charge of murine neuroblastoma cells (clone C1300-N18TG2). It was shown that the surface charge of these cells was determined mainly by anionic groups of the membrane which were distributed with density 0.2 e/nm3 in the layer covering its outer surface. The thickness of this layer was about 10 nm. These groups interacted with Ca ions (binding constant Kca-10-50 l/mol) and were titrated according to pK-3.8. Trypsin, neuraminidase and N-bromosuccinimide (which irreversibly neutralize the carboxylic groups of proteins) decreased the electrophoretic mobility of neuroblastoma cells while tosylchloride (a specific reagent for aminogroups) slightly increased it. The surface charge depended also on the conditions of cultivation of cell population. Morphological cell differentiation induced by removing the serum from the culture medium increased their mobility by about 30%. During the cultivation of cells in a medium with 10 or 50% of serum variations of the their mean electrophoretic mobility value were observed which were opposite-phase to the value of the daily increment of the number of cells. It was assumed that these effects were connected with partial self-synchronization of cell population. It is concluded that the surface charge of the neuroblastoma cells determined by microelectrophoresis method is mainly determined by the carboxylic groups of membrane periphery proteins and gangliosides and the content of these membrane components depends on the stage of cell development.
D000818:Animals; D001974:Bromosuccinimide / Q000494:pharmacology; D002999:Clone Cells; D004586:Electrophoresis; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008564:Membrane Potentials; D051379:Mice; D009439:Neuraminidase / Q000494:pharmacology; D009447:Neuroblastoma; D009474:Neurons / Q000502:physiology*; D014105:Tosyl Compounds / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
D014105:Tosyl Compounds; C025506:4-toluenesulfonyl chloride; D009439:Neuraminidase; D006821:Hyaluronoglucosaminidase; D014357:Trypsin; D001974:Bromosuccinimide
Poverkhnostnyĭ zariad kletok neiroblastomy myshi i ego izmenenie v protsesse rosta i morfologicheskoĭ differentsirovki kletochnoĭ populiatsii.