Phosphatidyl glycerolphosphate serves as glycerolphosphate donor in polymer synthesis.
Phosphatidyl glycerolphosphate was found to serve as the glycerolphosphate donor for polymer synthesis. When CDP-diglyceride and radiolabeled glycerolphosphate were incubated with the membrane enzyme prepared from Streptococcus sanguis, active syntheses of radiolabeled lipids and polymers were observed. The synthesis of polymer was not inhibited by low concentration of unlabeled phosphatidylglycerol. When [3H, 32P]glycerolphosphate was used, the polymer synthesized contained both 3H and 32P. The lipids formed were characterized as phosphatidylglycerol and phosphatidyl glycerolphosphate. The polymers formed from the latter were characterized as lipoteichoic acid like compounds by sodium dodecylsulfate-polyacrylamide gel electrophoresis.
D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D003567:Cytidine Diphosphate Diglycerides / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D010715:Phosphatidylglycerols / Q000378:metabolism*; D011108:Polymers / Q000378:metabolism*; D013298:Streptococcus sanguis / Q000201:enzymology
D003567:Cytidine Diphosphate Diglycerides; D010715:Phosphatidylglycerols; D011108:Polymers; C034358:phosphatidylglycerophosphate