A monoclonal antibody-defined membrane antigen complex is required for neutrophil-neutrophil aggregation.
We examined the aggregation responses of normal neutrophils treated with the murine monoclonal antibody (MoAb) 60.3. Addition of MoAb 60.3 to normal neutrophils produced dose-dependent inhibition of neutrophil aggregation in response to phorbol myristate acetate, zymosan-activated plasma, and N-formyl-methionylleucylphenylalanine. We conclude that the membrane glycoprotein complex recognized by MoAb 60.3--designated CDw18--is required for neutrophil-neutrophil aggregation in vitro.
D000911:Antibodies, Monoclonal / Q000502:physiology*; D000936:Antigen-Antibody Complex / Q000502:physiology*; D000954:Antigens, Surface / Q000276:immunology*; D001667:Binding, Competitive; D002449:Cell Aggregation; D006801:Humans; D009504:Neutrophils / Q000276:immunology / Q000502:physiology*
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000954:Antigens, Surface