Adenosine uptake sites in rat brain: identification using [3H]nitrobenzylthioinosine and co-localization with adenosine deaminase.
The binding characteristics of [3H]nitrobenzylthioinosine ([3H]NBI) to rat brain membrane preparations was examined, and the autoradiographic distribution of this ligand in brain sections was compared with the immunohistochemical localization of adenosine deaminase (ADA). It was found that [3H]NBI labels sites for which adenosine has far higher affinity than do other nucleosides, that these sites are heterogeneously distributed and that there is an exact correspondence between areas containing [3H]NBI sites and ADA-immunoreactive neurons. Our results indicate that [3H]NBI and ADA are potential markers for revealing anatomical sites at which actions of adenosine may be expressed.
D000241:Adenosine / Q000378:metabolism*; D000243:Adenosine Deaminase / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000201:enzymology*; D007288:Inosine / Q000031:analogs & derivatives*; D008297:Male; D009474:Neurons / Q000201:enzymology; D009700:Nucleoside Deaminases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013868:Thioinosine / Q000031:analogs & derivatives* / Q000378:metabolism
D013868:Thioinosine; D007288:Inosine; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase; C001789:4-nitrobenzylthioinosine; D000241:Adenosine