A method of isolating nick-translated DNA by subsequent separation on low-melting-temperature agarose.
The use of nick translation of cloned DNA segments followed by separation on low-melting-temperature agarose has been used to obtain multiple radiolabeled DNA probes from a single nick-translation procedure. This technique avoids gel matrix inhibition of enzymatic reactions. Examples of the utility of this procedure are presented and the advantages and drawbacks discussed.
D001345:Autoradiography; D055598:Chemical Phenomena; D002621:Chemistry; D003001:Cloning, Molecular; D004587:Electrophoresis, Agar Gel; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D014176:Protein Biosynthesis; D013816:Thermodynamics