A monoclonal-antibody-based immunoradiometric assay for apoprotein B in low-density lipoprotein.
Five different mouse monoclonal antibodies were raised against apoprotein B, the major protein in human low-density lipoproteins (LDL). Competitive binding studies suggested that these antibodies recognized four different epitopes on the lipoprotein particle. All antibodies bound to very-low-density and intermediate-density lipoproteins, which are the metabolic precursors of LDL and also contain apoprotein B. One antibody (P1C1) displayed a relative specificity for apoprotein B in LDL as compared with that in very-low-density lipoprotein and was used as a radiolabeled first antibody in an immunoradiometric assay for this lipoprotein. Measurements, either on isolated LDL or directly on plasma samples, demonstrated that this assay provides a rapid and precise method of quantification.
D000818:Animals; D000911:Antibodies, Monoclonal; D001055:Apolipoproteins B / Q000032:analysis* / Q000276:immunology; D001667:Binding, Competitive; D006801:Humans; D008077:Lipoproteins, LDL / Q000032:analysis* / Q000276:immunology; D008079:Lipoproteins, VLDL / Q000032:analysis / Q000276:immunology; D051379:Mice / Q000276:immunology; D008807:Mice, Inbred BALB C / Q000276:immunology; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D001055:Apolipoproteins B; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL