Studies of translatable mRNA for rabbit C-reactive protein.
C-reactive protein (CRP) mRNA was assayed by cell-free translation of poly(A)-containing liver RNA isolated both from rabbits stimulated to undergo the acute-phase response and from unstimulated control rabbits. No CRP-related translation products were identified until the denaturant methylmercury hydroxide (CH3HgOH) was added to the RNA before cell-free translation. In the presence of the denaturant, a 24000-Da translation product was synthesized which was immunochemically identifiable as the CRP primary translation product. It is likely that rabbit CRP mRNA can form a stable intramolecular duplex which interferes with its translatability in vitro. The 24000-Da CH3HgOH-facilitated cell-free translation product was not detected in poly(A)-containing liver RNA from unstimulated animals, indicating that the concentration of translatable CRP mRNA was dramatically induced during the acute-phase response. On the basis of absorption experiments, the 24000-Da CRP primary translation product was immunochemically more closely related to denatured CRP than to native CRP.
D000818:Animals; D002097:C-Reactive Protein / Q000235:genetics*; D002474:Cell-Free System; D011232:Chemical Precipitation; D004591:Electrophoresis, Polyacrylamide Gel; D008767:Methylmercury Compounds / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects; D011489:Protein Denaturation / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism
D008767:Methylmercury Compounds; D012333:RNA, Messenger; D002097:C-Reactive Protein; C020771:methylmercury hydroxide