Eukaryotic protein synthesis initiation factor eIF-3: determination of concentration and association with ribosomes in rabbit reticulocyte and HeLa cell lysates.
Monoclonal and polyclonal antibodies against eukaryotic protein synthesis initiation factor eIF-3 were produced and used to determine the factor concentration and its association with ribosomes in rabbit reticulocyte and HeLa cell lysates. In rabbit reticulocyte lysate we found 3-5 micrograms eIF-3 per mg total protein and in HeLa cell lysate 8-15 micrograms eIF-3 per mg total protein. The initiation factor eIF-3 was found both associated with 40 S ribosomal subunits and free in the post-ribosomal supernatant. However, no eIF-3 could be detected on mono- or polyribosomes.
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D004591:Electrophoresis, Polyacrylamide Gel; D039621:Eukaryotic Initiation Factor-3; D006367:HeLa Cells / Q000032:analysis*; D006801:Humans; D010448:Peptide Initiation Factors / Q000032:analysis* / Q000276:immunology; D011817:Rabbits; D011863:Radioimmunoassay; D012156:Reticulocytes / Q000032:analysis*; D012270:Ribosomes / Q000032:analysis*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D039621:Eukaryotic Initiation Factor-3; D010448:Peptide Initiation Factors