[Conditions for porphyrin formation by propionic acid bacteria].
Porphyrin production by seven species of propionic acid bacteria (Propionibacterium shermanii, its mutant P. shermanii M-82, P. technicum, P. vannielii, P. rubrum, P. thoenii and P. jensenii) was being studied. All the bacteria were cultivated on a glucose-peptone medium. A positive correlation between the amount of the produced porphyrins and the vitamin B12-synthetizing activity was observed for the most of species. Exogenous delta-aminolevulinic acid stimulated the porphyrin accumulation, but the degree of its utilisation decreased as its content in the culture medium increased from 5 to 200 mg/l. A maximum synthesis of porphyrins by P. shermanii M-82 (mainly of coproporpyrin III) was observed at definite concentrations of glucose and cobalt salts.
D002855:Chromatography, Thin Layer; D003470:Culture Media / Q000378:metabolism; D007536:Isomerism; D009154:Mutation; D011166:Porphyrins / Q000032:analysis / Q000096:biosynthesis*; D011424:Propionibacterium / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D014805:Vitamin B 12 / Q000032:analysis / Q000096:biosynthesis
D003470:Culture Media; D011166:Porphyrins; D014805:Vitamin B 12
Usloviia obrazovaniia profirinov propionovokislymi bakteriiami.