Complete amino acid sequence of the Aspergillus cytotoxin mitogillin.
The complete amino acid sequence of the cytotoxin mitogillin has been determined by sequencing the intact chain and peptide fragments produced by cleavage at methionyl, arginyl, lysyl, and tryptophanyl residues and at one aspartic acid-proline bond. The protein consists of 149 amino acid residues with alanine at the NH2 terminus and histidine at the COOH terminus. The calculated Mr of the native mitogillin was 16 867. The native molecule presents two disulfide bridges, one between cysteine residues at positions 5 and 147 and another one between cysteine residues at positions 75 and 131. The amino acid sequence of mitogillin shows 86% homology with another cytotoxic protein called alpha-sarcin.
D000485:Allergens; D000595:Amino Acid Sequence; D052179:Antigens, Plant; D001230:Aspergillus; D002250:Carbon Radioisotopes; D002268:Carboxypeptidases; D043422:Carboxypeptidases A; D002851:Chromatography, High Pressure Liquid; D003488:Cyanogen Bromide; D003603:Cytotoxins; D005656:Fungal Proteins; D007202:Indicators and Reagents; D007461:Iodoacetates; D019807:Iodoacetic Acid; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000032:analysis; D010455:Peptides; D012260:Ribonucleases; D014357:Trypsin
C081947:ASPF1 protein, Aspergillus fumigatus; D000485:Allergens; D052179:Antigens, Plant; D002250:Carbon Radioisotopes; D003603:Cytotoxins; D005656:Fungal Proteins; D007202:Indicators and Reagents; D007461:Iodoacetates; D010446:Peptide Fragments; D010455:Peptides; C036196:MITF protein, Aspergillus restrictus; D012260:Ribonucleases; D002268:Carboxypeptidases; D043422:Carboxypeptidases A; D014357:Trypsin; D003488:Cyanogen Bromide; D019807:Iodoacetic Acid