Immunoassay of 6-hydroxymelatonin sulfate in human plasma and urine: abolition of the urinary 24-hour rhythm with atenolol.
An assessment of the rhythmic characteristics of melatonin secretion in man and other species requires the determination of 24-h secretion profiles. Measurement of a major excreted metabolite would allow noninvasive study of pineal function, applicable in particular to pediatric and long term circadian rhythm studies. This report describes a simple and rapid RIA for 6-hydroxymelatonin sulfate in human plasma and urine. Physiological studies revealed that both plasma and urinary levels of 6-hydroxymelatonin sulfate were closely related to plasma melatonin, and that the urinary 24-h rhythm was abolished by the beta 1-adrenergic anagonist atenolol.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D001262:Atenolol / Q000494:pharmacology*; D002854:Chromatography, Paper; D002940:Circadian Rhythm; D003429:Cross Reactions; D005260:Female; D006801:Humans; D007118:Immunoassay; D008099:Liver / Q000378:metabolism; D008550:Melatonin / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000378:metabolism / Q000652:urine; D051381:Rats; D011919:Rats, Inbred Strains
D000906:Antibodies; D001262:Atenolol; D008550:Melatonin; C012365:6-hydroxymelatonin