Fractionation of chromatin fragments on columns of Biogel A50-m at different salt concentrations.
Nuclease fragmented chromatin was chromatographed on Biogel at various NaCl concentrations. The yield of eluted chromatin, and its H1/core histone ratio was minimal at 0.18 M NaCl where the ratio of H1 subtypes H1c/H1ab was maximal. Therefore, the eluted material was aggregation-resistant chromatin while aggregatable chromatin remained on the columns. Previous results were interpreted as H1 depletion of chromatin by ion-exchange properties of Biogel, but the primary phenomenon is now seen as a separation of classes of chromatin that differ in sensitivity to salt-induced aggregation. At very low salt concentrations, Biogel chromatography can be used without concern for H1 depletion.
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000032:analysis; D002843:Chromatin / Q000032:analysis*; D002850:Chromatography, Gel / Q000379:methods; D006657:Histones / Q000032:analysis; D008836:Micrococcal Nuclease / Q000378:metabolism; D009994:Osmolar Concentration; D012965:Sodium Chloride; D012996:Solutions; D013950:Thymus Gland / Q000648:ultrastructure
D002843:Chromatin; D006657:Histones; D012996:Solutions; D012965:Sodium Chloride; D008836:Micrococcal Nuclease