Effect of erythropoietic stimulation of the chamber host on the growth of haemopoietic colonies in plasma clot diffusion chambers (PCDC).
Murine marrow cells were cultured in Millipore diffusion chambers implanted into the peritoneal cavity of variously conditioned murine hosts. Preirradiation (350 cGy), bleeding (0.5 ml) and phenylhydrazine injection (75 mg/kg i.v.) when performed together on the chamber host, induced better growth of erythropoietic and granulopoietic colonies inside the PCDCs than either of these manoeuvres alone. Small erythrocytic colonies (CFU-E derived) and small granulocytic colonies were observed at day 3 of marrow culture. Erythropoietic bursts and large granulocytic colonies were observed at day 8 of chamber culture. Colonies of macrophage-like cells, fibroblast-like cells, mixed erythro-granulopoietic colonies and megakaryoblasts were observed less regularly in chambers incubated in these conditions. The study provides a standardized, relatively reproducible PCDC culture system for studies of both erythro- and granulopoiesis, and does not require a hypoxic chamber.
D000818:Animals; D001854:Bone Marrow Cells; D002455:Cell Division; D002478:Cells, Cultured; D004058:Diffusion; D004920:Erythropoiesis; D005260:Female; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D051379:Mice; D010659:Phenylhydrazines / Q000494:pharmacology
D010659:Phenylhydrazines; C030299:phenylhydrazine