[Role of calcium-dependent and calcium-independent mechanisms in the adhesion of retinal and pigment epithelium cells in the chick embryo].
The mechanisms of adhesion of the retinal and pigment epithelium cells, as well of cell interaction within each of these tissues were studied during development. It was shown by means of separation of retina from pigment epithelium in different dissociation media that the adhesion of these tissues in 5-6 day old chick embryos is realized via a Ca2+-independent mechanism. The adhesion of these tissues decreases between days 7 and 16. Starting from day 16, both Ca2+-independent and Ca2+-dependent mechanisms are involved in the interaction of the retinal and pigment epithelium cells. By measuring the output of single cells into the suspension after the treatment of retina and pigment epithelium with different dissociating agents, it was shown that from the 5th day of incubation on the adhesion of pigment epithelium cells is mediated by Ca2+-dependent mechanism. In the retina three types of cells were found: interacting via Ca2+-dependent mechanism only, Ca2+-independent mechanism only, and both the mechanisms. In the course of differentiation, the numbers of the population of cells interacting only via Ca2+-dependent mechanism increase, while those of cells interacting via Ca2+-independent mechanism decrease. It is suggested that at each developmental stage those retinal cell possess Ca2+-dependent mechanism of adhesion which are closest to the definitive state.
D000818:Animals; D002118:Calcium / Q000502:physiology*; D002448:Cell Adhesion; D002450:Cell Communication; D002469:Cell Separation; D002478:Cells, Cultured; D002642:Chick Embryo / Q000166:cytology*; D010857:Pigment Epithelium of Eye / Q000166:cytology*; D012160:Retina / Q000166:cytology*; D013997:Time Factors
D002118:Calcium
Rol' kal'tsiĭ-zavisimogo i kal'tsiĭ-nezavisimogo mekhanizmov v adgezii kletok setchatki i pigmentnogo épiteliia kurinykh zarodysheĭ.