[Role of adipocyte precursors in the initiation of nutritional obesity before weaning].
The stroma vascular fraction of adipose tissue partly consists of adipose precursor cells which can convert into adipocytes in vitro. The aim of this study was to investigate the possible contribution of cells from the stroma vascular compartment to the initiation of obesity induced by overfeeding during the early neonatal weeks in rats. Overfeeding during the suckling period was obtained by reducing the litter size. The inguinal adipose tissue of overfed rats raised in litters of 4 pups each was overdeveloped compared to that of controls raised in litters of 8 pups each, and the difference between the two groups became significant as early as 10 days of age. At this age, adipose tissue enlargement was only due to adipocyte hypertrophy; afterwards, hyperplasia of the mature fat cells contributed to the overdevelopment of adipose tissue in 15-day old overfed rats. The cell number in the stroma vascular fraction increased in the overfed group as early as 10 days of age and thus preceded the onset of mature fat cell hyperplasia. The developmental pattern of lipoprotein lipase, glycerol-3-phosphate dehydrogenase, glycerol-acyl-transferase and acyl-CoA ligase activities in stromal cells did not depend on litter size, but specific enzyme activities wee increased in 10-day old overfed rats compared to the controls. These results indicate that early overfeeding induced cell proliferation in the stroma vascular compartment and also induced the enzyme activities involved in adipose conversion to increase in these cells. This strongly suggests that precursor cell differentiation was greater in overfed rats than in control rats.
D000273:Adipose Tissue / Q000473:pathology* / Q000503:physiopathology; D000818:Animals; D000831:Animals, Newborn / Q000033:anatomy & histology*; D000833:Animals, Suckling; D002454:Cell Differentiation; D003066:Coenzyme A Ligases / Q000378:metabolism; D004032:Diet; D005992:Glycerol-3-Phosphate O-Acyltransferase / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D006984:Hypertrophy; D008071:Lipoprotein Lipase / Q000378:metabolism; D009765:Obesity / Q000209:etiology / Q000473:pathology* / Q000503:physiopathology; D051381:Rats; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D000098859:Long-Chain-Fatty-Acid-CoA Ligase
D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D005993:Glycerolphosphate Dehydrogenase; D005992:Glycerol-3-Phosphate O-Acyltransferase; D008071:Lipoprotein Lipase; D003066:Coenzyme A Ligases; C090799:FAA2 protein, S cerevisiae; D000098859:Long-Chain-Fatty-Acid-CoA Ligase
Rôle des précurseurs d'adipocytes dans l'installation d'une obésité nutritionnelle avant le sevrage.