A steady-state gel filtration method on micro-columns for the measurement of percentage free testosterone in serum.
A method is described for measuring the percentage of free testosterone in serum using steady-state gel filtration on micro-columns of Sephadex G50 (fine). The method has a coefficient of variation of 4.0% and up to 20 samples can be processed in a day. Reference ranges have been established for normal women at different stages of their menstrual cycles and for normal men; there is good agreement with values published by workers using other methods. This method combined with a specific and precise method for measuring total testosterone opens up the possibility for the routine measurement of free testosterone concentration in serum.
D000328:Adult; D002850:Chromatography, Gel / Q000295:instrumentation; D005260:Female; D006801:Humans; D008297:Male; D008597:Menstrual Cycle; D008832:Microchemistry; D012016:Reference Values; D013739:Testosterone / Q000097:blood*
D013739:Testosterone