Cerebellar nuclear afferents from feline hypothalamus demonstrated by retrograde transport after implantation of crystalline wheat germ agglutinin-horseradish peroxidase complex.
A hypothalamo-cerebellar projection has recently been discovered in the cat, and its presence has been verified in other mammalian species. Due to methodological limitations only hypothalamic projections to cerebellar cortex had been demonstrated. By means of modification of the new implantation technique described by Mori et al. [10], hypothalamic projections to the cerebellar nuclei have been revealed using retrograde transport of a wheat germ agglutinin-horseradish peroxidase complex (WGA-HRP). This method permits identification of afferents to restricted nuclear regions without uptake of the tracer into passing fibers. The projection from hypothalamus has an ipsilateral predominance and has its main terminal area in the fastigial nucleus. These findings were verified in anterograde transport experiments after hypothalamic injections of WGA-HRP.
D000818:Animals; D002415:Cats; D002529:Cerebellar Nuclei / Q000033:anatomy & histology*; D007031:Hypothalamus / Q000033:anatomy & histology*; D009434:Neural Pathways / Q000033:anatomy & histology