Quantitation of the transgenome size in chromosome-mediated gene transfer lines.
Twenty-two HPRT-selected chromosome-mediated gene transfer lines were characterized by quantitative "dot" blotting. The range of human sequences in these lines extended from over 120,000 kb to less than 5,000 kb. One-half of these lines carried less than 16,000 kb.
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D005796:Genes; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics; D006367:HeLa Cells / Q000201:enzymology; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics; D007739:L Cells / Q000201:enzymology; D051379:Mice; D009693:Nucleic Acid Hybridization; D010735:Phosphoglycerate Kinase / Q000235:genetics
D000954:Antigens, Surface; D007527:Isoenzymes; D005954:Glucosephosphate Dehydrogenase; D007041:Hypoxanthine Phosphoribosyltransferase; D010735:Phosphoglycerate Kinase