Determination of total intracellular lipid content by flow cytofluorometry.
It was demonstrated that perylenoyl-labeled triglyceride, rac-1,2-dioleoyl-3-[9(3-perylenoyl)nonanoyl]glycerol (PLTG), specifically stains the intracellular lipids in formalin-fixed and non-fixed cells in situ and in suspension. The fluorescence of labeled cells was registered on a FACS-II cytofluorometer. It was found that the mean intensity of fluorescence in PLTG-labeled cells of various types, both fixed and non-fixed, correlates with their total lipid content. There was no correlation, however, between the mean intensity of fluorescence and intracellular levels of lipids belonging to different classes: phospholipids, triglycerides, cholesterol or cholesteryl esters.
D000818:Animals; D001826:Body Fluids / Q000032:analysis*; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000032:analysis; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006801:Humans; D007424:Intracellular Fluid / Q000032:analysis*; D008055:Lipids / Q000032:analysis*; D051379:Mice; D051381:Rats; D014280:Triglycerides
D005456:Fluorescent Dyes; D008055:Lipids; D014280:Triglycerides; C045507:1,2-dioleoyl-3-(9-(3-perylenoyl)nonanoyl)glycerol