3-Hydroxyacyl-CoA epimerase is a peroxisomal enzyme and therefore not involved in mitochondrial fatty acid oxidation.
The subcellular location of 3-hydroxyacyl-CoA epimerase (EC 5.1.2.3) was studied by differential centrifugation and Percoll density gradient centrifugation of a rat liver homogenate. The enzyme was found to be associated with peroxisomes but not with mitochondria. This observation proves that 3-hydroxy-acyl-CoA epimerase does not function in mitochondrial beta-oxidation of polyunsaturated fatty acids which are degraded by a modified pathway.
D000818:Animals; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D002994:Clofibrate / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D005227:Fatty Acids / Q000378:metabolism*; D007535:Isomerases / Q000032:analysis*; D008099:Liver / Q000201:enzymology / Q000648:ultrastructure*; D008297:Male; D008830:Microbodies / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000201:enzymology*; D010084:Oxidation-Reduction; D019998:Racemases and Epimerases / Q000032:analysis* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D005227:Fatty Acids; D007535:Isomerases; D019998:Racemases and Epimerases; C029601:3-hydroxybutyryl-CoA epimerase; D002994:Clofibrate