Isolation and characterization of Streptomyces venezuelae mutants blocked in chloramphenicol biosynthesis.
Twelve Streptomyces venezuelae mutants blocked in chloramphenicol biosynthesis were isolated. Two of these (Cm1-1 and Cm1-12) were apparently blocked in the conversion of chorismic acid to p-aminophenylalanine and three (Cm1-4, Cm1-5 and Cm1-8) accumulated p-aminophenylalanine and may have been blocked in the hydroxylation reaction that converted this intermediate to p-aminophenylserine. One mutant (Cm1-2) accumulated D-threo-1-p-nitrophenyl-2-propionamido-1,3-propanediol and D-threo-1-p-nitrophenyl-2-isobutyramido-1,3-propanediol, indicating that chlorination of the alpha-N-acyl group of chloramphenicol was blocked. The remaining six strains did not excrete any detectable chloramphenicol pathway intermediates.
D002701:Chloramphenicol / Q000096:biosynthesis*; D002827:Chorismic Acid / Q000378:metabolism; D002855:Chromatography, Thin Layer; D009154:Mutation; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
D002701:Chloramphenicol; D002827:Chorismic Acid