Qualitative and quantitative analysis of ranitidine and its metabolites by high-performance liquid chromatography-mass spectrometry.
Reversed-phase high-performance liquid chromatography systems for the separation of ranitidine and its metabolites ranitidine-N-oxide, ranitidine-S-oxide, and desmethylranitidine have been developed for use in high-performance liquid chromatography-mass spectrometry. A direct liquid introduction-high-performance liquid chromatography-mass spectrometry system to analyse qualitatively and quantitatively solutions containing ranitidine and its metabolites by reversed-phase chromatography is described. A sample of urine collected from a subject given an oral dose of 75 mg of ranitidine and 75 mg of tris-deuterated ranitidine was analysed by this system. Ranitidine and its metabolites were identified by the ion doublets in the mass spectra which were 3 a.m.u. apart.
D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007700:Kinetics; D013058:Mass Spectrometry; D011899:Ranitidine / Q000031:analogs & derivatives / Q000032:analysis* / Q000097:blood; D013056:Spectrophotometry, Ultraviolet
C045908:5-desmethylranitidine; C045907:ranitidine-S-oxide; D011899:Ranitidine; C038169:ranitidine N-oxide