Retention and biliary excretion of poly-alpha, beta-[N(2-hydroxyethyl)-D,L-aspartamide] (PHEA) and its tyramine derivative (PHEA-Tyr) by isolated perfused rat liver. The role of molecular weight and chemical structure.
Isolated rat liver was perfused with fractions of fluorescent labelled poly-alpha,beta-[N(2-hydroxyethyl)-D,L-aspartamide] (PHEA), (Mw:8000, 51 400 and 70 000) and its tyramine derivative, PHEA-Tyr (19 mol% tyramine side chains; Mw:8000 and 35 000) under conditions approximating an "in vivo" situation. By an analysis of the recovered polymers it was shown, firstly that accumulation of polymers, which occurred most apparently in the Kupffer cells, was neither affected by the presence of tyramine side chains nor influenced by the molecular weight of polymers; secondly that the tyramine side chains slightly increased the biliary excretion of PHEA-Tyr, and thirdly with both types of polymers the excretion of high-molecular-weight fractions in the bile was strongly suppressed.
D000818:Animals; D001646:Bile / Q000378:metabolism*; D007728:Kupffer Cells / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D010455:Peptides / Q000378:metabolism*; D010477:Perfusion; D010873:Pinocytosis; D011108:Polymers / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012710:Serum Albumin, Bovine; D013329:Structure-Activity Relationship; D013667:Technetium
D010455:Peptides; D011108:Polymers; C046050:alpha,beta poly((2-hydroxyethyl)aspartamide)tyramine; D012710:Serum Albumin, Bovine; D013667:Technetium