Development of an affinity chromatography resin for the purification of carcinogen binding proteins from mouse liver.
Pyrene, a structural analog of benzo[a]pyrene, is an effective competing ligand for high affinity carcinogen binding proteins in mouse liver. A pyrene-derivatized Sepharose gel was prepared for affinity chromatography purification of these proteins, and adsorbs all detectable [3H]B[a]P-binding activity from hepatic cytosol with the adsorption of less than 1% of total protein. Specific carcinogen binding activity is recovered from pyrene-derivatized Sepharose columns with the enrichment of a 33 kDa polypeptide. This chromatography resin represents a major step in the isolation of these unusual receptor-like binding proteins for aromatic hydrocarbon carcinogens.
D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism; D001667:Binding, Competitive; D002273:Carcinogens; D002352:Carrier Proteins / Q000302:isolation & purification*; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000032:analysis*; D008297:Male; D051379:Mice
D002273:Carcinogens; D002352:Carrier Proteins; D001564:Benzo(a)pyrene