Purification, affinity to anti-human arginase immunoglobulin-Sepharose 4B and subunit molecular weights of mammalian arginases.
The affinities of anti-human liver arginase antibodies raised in rabbits to liver arginases from man, bovine, pig, dog, guinea pig, rat and mouse were investigated by Scatchard analysis of the binding of the arginases from crude liver extracts to Sepharose-bound immunoglobulins. All arginases bound with good affinity, but the binding capacities of the immunosorbent for the enzymes from various species decreased with decreasing phylogenetic relationship of the species. Arginase from murine peritoneal macrophages did not bind to the immunosorbent at all. A simple two-step purification method for the liver arginases of all species mentioned above is given. All arginases were purified to electrophoretical homogeneity. The molecular weights of their subunits were estimated.
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D001119:Arginase / Q000302:isolation & purification* / Q000378:metabolism; D002417:Cattle; D002846:Chromatography, Affinity; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D006168:Guinea Pigs; D006801:Humans; D007120:Immunochemistry; D007700:Kinetics; D046911:Macromolecular Substances; D008264:Macrophages / Q000201:enzymology; D051379:Mice; D008970:Molecular Weight; D051381:Rats; D012685:Sepharose; D013045:Species Specificity; D013552:Swine
D000906:Antibodies; D000936:Antigen-Antibody Complex; D046911:Macromolecular Substances; D012685:Sepharose; D001119:Arginase