A 46-nucleotide promoter segment from an IFN-alpha gene renders an unrelated promoter inducible by virus.
A hybrid gene consisting of the human IFN-alpha 1 promoter and a beta-globin transcription unit is expressed correctly only after viral induction. To determine the region required for inducibility, 25 hybrid promoters consisting of varying upstream IFN-alpha 1 and downstream beta-globin promoter moieties were analyzed, and 5'-deletion analysis was performed on an inducible hybrid promoter. An IFN promoter region from position -109 to -64 conferred maximal inducibility on downstream beta-globin promoter segments and even on the intact beta-globin promoter. This region is strikingly conserved among human IFN-alpha and -beta genes. As constitutive expression of the beta-globin gene was not diminished by placing IFN promoter fragments in various positions, induction is attributed largely to positive, rather than to negative control.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002872:Chromosome Deletion; D004274:DNA, Recombinant; D005914:Globins / Q000235:genetics; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D009522:Newcastle disease virus / Q000502:physiology*; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D007370:Interferon Type I; D005914:Globins