Colorimetric analysis of some diuretic drugs: hydrochlorothiazide and spironolactone.
Two convenient spectrophotometric methods were developed for the determination of hydrochlorothiazide and spironolactone. A specific colour reaction for the determination of hydrochlorothiazide is reported. One method is based on the reaction of hydrochlorothiazide with n-butylamine and cobaltous chloride in anhydrous methanol. A blue-violet colour is produced and is measured at 570 nm. A highly selective colorimetric method was adopted for the analysis of spironolactone by reacting with isoniazid forming a coloured hydrazone derivative and subsequent measurement of the coloured product at 405 nm.
D055598:Chemical Phenomena; D002621:Chemistry; D003035:Cobalt; D003124:Colorimetry; D006852:Hydrochlorothiazide / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D013148:Spironolactone / Q000032:analysis*; D013607:Tablets; D013997:Time Factors
D013607:Tablets; D006852:Hydrochlorothiazide; D013148:Spironolactone; D003035:Cobalt; C018021:cobaltous chloride