Computerized analysis of TV images for ultrasensitive monitoring of the reaction of fluorochrome with protein.
Preparation of protein-specific fluorescent probes with the desired degree of fluorochrome can be greatly facilitated by a technique that combines thin-layer chromatography (TLC) with quantitative image analysis (QIA). Using TLC/QIA, the investigator can determine the fluorochrome/protein ratio on-line with only a few micrograms of protein as fluorochrome is conjugated to protein. In addition, this technique allows rapid quantitation of dye noncovalently adsorbed to fluorochrome-labeled protein.
D055598:Chemical Phenomena; D002621:Chemistry; D002855:Chromatography, Thin Layer / Q000295:instrumentation; D003201:Computers; D004591:Electrophoresis, Polyacrylamide Gel; D005453:Fluorescence; D005456:Fluorescent Dyes; D007074:Immunoglobulin G / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D013690:Television; D013997:Time Factors
D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D011506:Proteins