A home-made solid-phase sequencer operating at the nanomole level.
The building and functioning of a fully automated solid-phase sequencer is described. The peptide is coupled via its alpha-carboxyl end to activated glass beads and successively reacted with Chang's and Edman's reagents. All operations are electronically controlled by the automated programmer. All components necessary to build the machine are commercially available. This sequencer has been used at a nanomole level in the final phase of a protein sequence determination. The overall cost as well as the sensitivity and efficiency of the final product compare favourably to those of commercial machines.
D000478:Alkylation; D000595:Amino Acid Sequence; D001226:Aspartate-tRNA Ligase / Q000032:analysis; D001322:Autoanalysis / Q000295:instrumentation*; D003201:Computers; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000032:analysis; D011506:Proteins
D010446:Peptide Fragments; D011506:Proteins; D001226:Aspartate-tRNA Ligase