Effects of factors derived from a tumor clonal cell line on DNA synthesis of transformed and non transformed cells.
Conditioned medium from neoplastic thyroid cell cultures, extracts of tumors developed by identical cells in isogeneic Fisher 344 rats and serum from those tumor-bearing animals, were tested in pulse thymidine labelled experiments on a transformed and two non transformed cell lines. Tumor extract and conditioned medium inhibited DNA synthesis. Tumor-bearing rat serum increased DNA synthesis in a cerebellar transformed cell line, but no in chick embryo fibroblasts or in aorta non transformed cells.
D000818:Animals; D001769:Blood; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002531:Cerebellum / Q000187:drug effects; D002642:Chick Embryo; D003470:Culture Media; D004261:DNA Replication / Q000187:drug effects*; D051381:Rats; D011916:Rats, Inbred F344; D013964:Thyroid Neoplasms / Q000032:analysis*; D014020:Tissue Extracts / Q000494:pharmacology*
D003470:Culture Media; D014020:Tissue Extracts