Complete sulfation of jejunal gastrin in the human fetus.
The degree of tyrosine-O-sulfation and the ratio between large (gastrin-34 and component I) and small (gastrin-17 and -14) molecular forms of gastrin were studied in extracts of human fetal (n = 14) and adult (n = 9) antrum, duodenum, jejunum and pancreas. Boiled water extracts were applied to gel- and ion-exchange chromatography before and after treatment with trypsin and arylsulfatase. The fractions were monitored with sequence-specific radioimmunoassays that distinguish sulfated from non-sulfated gastrins. In antrum and duodenum about half the gastrins were sulfated at all stages of development. In the fetal jejunum gastrin occurred in sulfated form only while in the adult 72% (range, 64-88%) of the jejunal gastrins were sulfated. The larger molecular forms of gastrin predominated in the fetal compared with the adult antrum. In duodenum and jejunum, however, the ratio between small and large forms was the same in fetus and adult. Gastrin was undetectable in both fetal and adult pancreas. The results show that the degree of sulfation of gastrin varies substantially in the different parts of the gut at different stages of development. The differences may have functional significance, since sulfation increases the pancreozyminic and cholecystokinetic potency of gastrin.
D000328:Adult; D002852:Chromatography, Ion Exchange; D005260:Female; D005333:Fetus / Q000378:metabolism*; D005755:Gastrins / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007583:Jejunum / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D011247:Pregnancy; D011863:Radioimmunoassay; D013431:Sulfates / Q000378:metabolism*
D005755:Gastrins; D013431:Sulfates