Evidence for production of 1-acyl-2-acetyl-sn-glyceryl-3-phosphorylcholine concomitantly with platelet-activating factor.
The presence of 1-acyl-2-acetyl-sn-glyceryl-3-phosphorylcholine in a sample of platelet-activating factor from stimulated rabbit neutrophils was demonstrated by a gas-liquid chromatography/mass spectrometry technique coupled with selected ion monitoring. The ions chosen for identification were those of acetyl and long-chain acyl moieties and molecular weight. Species containing palmitic, oleic and stearic acids were detected. A good correlation was observed between the productions of 1-acyl-2-acetyl-sn-glyceryl-3-phosphorylcholine and 1-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine by neutrophils stimulated with ionophore A23187.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D008401:Gas Chromatography-Mass Spectrometry; D066298:In Vitro Techniques; D008297:Male; D009504:Neutrophils / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000031:analogs & derivatives* / Q000096:biosynthesis*; D011817:Rabbits; D012015:Reference Standards
D010972:Platelet Activating Factor