Biphasic regulation of detergent-solubilized muscarinic acetylcholine receptors from rat cerebral cortex by N-ethylmaleimide.
Muscarinic acetylcholine receptors were solubilized from rat cerebral cortex with 1% digitonin. Treatment with N-ethylmaleimide decreased or increased agonist affinity for the receptor, depending on the extent of alkylation. This indicates that certain determinants of receptor state do not depend on interactions with other membrane structures.
D000818:Animals; D001923:Brain Chemistry; D002217:Carbachol / Q000378:metabolism; D004074:Digitoxin / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005033:Ethylmaleimide / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D008566:Membranes / Q000378:metabolism; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011976:Receptors, Muscarinic / Q000378:metabolism*; D012995:Solubility
D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate; D002217:Carbachol; D004074:Digitoxin; D005033:Ethylmaleimide; D004229:Dithiothreitol