Measurement of macrophage cellular procoagulant activity.
We developed a simple technique for the measurement of the procoagulant activity exposed on the surface of macrophages. The cells are isolated, adhered to plastic surfaces, and assayed in the same device. This approach allows us to study the microcoagulation on the surface of intact macrophages by sensitive and specific clotting tests.
D000818:Animals; D001202:Ascitic Fluid; D001777:Blood Coagulation; D001779:Blood Coagulation Factors / Q000032:analysis*; D002478:Cells, Cultured; D046508:Culture Techniques / Q000295:instrumentation; D004731:Endotoxins / Q000494:pharmacology; D005260:Female; D008264:Macrophages / Q000032:analysis* / Q000187:drug effects; D011650:Pulmonary Alveoli / Q000166:cytology; D051381:Rats; D013154:Spleen / Q000166:cytology; D013925:Thromboplastin / Q000494:pharmacology; D014812:Vitamin K / Q000494:pharmacology; D014859:Warfarin / Q000494:pharmacology
D001779:Blood Coagulation Factors; D004731:Endotoxins; C045072:macrophage procoagulant activity; D014812:Vitamin K; D014859:Warfarin; D013925:Thromboplastin