[R-factor from the natural strain of Salmonella derby carrying a DNA-polymerase gene].
A R-factor which determines multiple stability to antibiotics (Cm, Pn, Sm) was found in a Salmonella derby strain isolated from the clinical material. The plasmid was eliminated by treatment with ethidium bromide; the DNA-polymerase activity in the antibiotic-sensitive derivatives measured under conditions optimal for DNA-polymerase I from E. coli was found to be decreased 10-50-fold. Plasmid DNA of S. derby K89 was fractionated by electrophoresis in agarose gel; individual zones I-IV were obtained, using a preparative technique. Upon transformation of S. derby K82 pol- cells, only plasmid DNA in zone II (designed as pSD Cm pol) gave Cm-resistant transformants, in which the DNA-polymerase activity decreased to the normal level. The experimental results pont to the binding of the DNA-polymerase gene to the S. derby plasmid.
D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005796:Genes; D005798:Genes, Bacterial; D011815:R Factors; D012475:Salmonella / Q000235:genetics*; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D004259:DNA-Directed DNA Polymerase
R-faktor iz prirodnogo shtamma Salmonella derby, nesushchiĭ gen DNK-polimerazy.