31P nuclear magnetic resonance studies of crayfish (Orconectes virilis). The use of inversion spin transfer to monitor enzyme kinetics in vivo.
31P nuclear-magnetic resonance (NMR) has been used to observed in vivo the steady-state levels of phosphorus-containing metabolites in the crayfish Orconectes virilis and the intracellular pH of the abdominal muscle was determined. Measurement of spin-lattice relaxation times and spin transfer experiments have enabled calculation of unidirectional rate constants and activation energies for the arginine kinase reaction in vivo.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000378:metabolism; D003400:Astacoidea / Q000378:metabolism*; D004734:Energy Metabolism; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008433:Mathematics; D008956:Models, Chemical; D009943:Organophosphorus Compounds / Q000378:metabolism; D010084:Oxidation-Reduction; D013696:Temperature
D009943:Organophosphorus Compounds; C015441:phospho-L-arginine; D000255:Adenosine Triphosphate; D001120:Arginine