Evaluation of a double-tracer autoradiographic technique for the measurement of both local cerebral glucose metabolism and local cerebral blood flow.
A solvent washout technique is evaluated that could be used to remove one 14C tracer after a combined autoradiographic CMRglu and CBF study using [14C]2-deoxyglucose (2DG) and [14C]iodoantipyrine (IAP). The technique permits the simultaneous measurement of local CBF and local CMRglu in the same animal without the use of the short-lived tracers of iodine, 123I and 131I, for iodoantipyrine labeling. This report shows that brain tissue sections did not retain 14C from [14C]2DG when chloroform was used as the washout solvent. Chloroform washing removes nearly all the 14C from [14C]IAP. For this study, careful attention was given to the control, prewash measurement of 14C concentration.
D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives; D001345:Autoradiography; D001921:Brain / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002560:Cerebrovascular Circulation; D003847:Deoxyglucose; D005947:Glucose / Q000378:metabolism*
D002250:Carbon Radioisotopes; D003847:Deoxyglucose; D005947:Glucose; D000983:Antipyrine; C008135:iodoantipyrine