Platelet-stimulating and membranolytic properties of racemic PAF-acether and analogues.
Three synthetic 1-O-hexadecyl-rac-glycero-3-phosphocholines and the corresponding 1-O-hexadecyl-rac-glycero-3-phosphoric acid methyl esters were tested for platelet-aggregatory and membranolytic properties. Nanomolar concentrations of both the 2-O-acetyl and the 2-O-propionyl derivative of 1-O-hexadecyl-rac-glycero-3-phosphocholine were shown to activate platelets in human platelet-rich plasma. About 625-fold higher concentrations of the corresponding phosphoric acid methyl esters are required to produce the same platelet-stimulating effect. The 1-O-hexadecyl-rac-glycero-3-phosphocholine and the 1-O-hexadecyl-rac-glycero-3-phosphoric acid methyl ester are both nearly ineffective up to final concentrations of 0.1 to 0.2 mM. All tested compounds also cause a graduated lysis of red cells in the range of micromolar concentrations.
D001792:Blood Platelets / Q000187:drug effects*; D002462:Cell Membrane / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008244:Lysophosphatidylcholines / Q000494:pharmacology; D010743:Phospholipids / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000031:analogs & derivatives / Q000494:pharmacology / Q000502:physiology*; D010974:Platelet Aggregation / Q000187:drug effects; D012701:Serotonin / Q000494:pharmacology
D008244:Lysophosphatidylcholines; D010743:Phospholipids; D010972:Platelet Activating Factor; C006065:We 201; D012701:Serotonin; C037145:1-hexadecyl-2-acetyl-glycero-3-phosphocholine