Use of a urea and guanidine-HCl-propanol solvent system to purify a growth inhibitory glycopeptide by high-performance liquid chromatography.
Reversed-phase high-performance liquid chromatography was used to purify an inhibitory glycopeptide where resolution and recovery were enhanced by using urea or guanidine-HCl-isopropanol-water as a solvent system. Isopropanol alone or other solvent systems that have been proposed for such purification steps were not effective in eluting hydrophobic proteins from the reversed-phase column. The application of the urea or guanidine-HCl solvent systems in the separation and purification of membrane proteins, and other hydrophobic macromolecules, could greatly enhance recovery and efficiency of purification.
D000433:1-Propanol; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D006131:Growth Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D019791:Guanidine; D006146:Guanidines; D008565:Membrane Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D012997:Solvents; D014508:Urea
D006131:Growth Inhibitors; D006146:Guanidines; D008565:Membrane Proteins; D012997:Solvents; C031717:growth inhibitory proteins; D014508:Urea; D000433:1-Propanol; D019791:Guanidine