A novel type of Chinese hamster ovary cell mutant defective in oligosaccharide-lipid synthesis.
A novel type of Chinese hamster ovary cell mutant has been isolated with a constitutive defect in the synthesis of lipid-linked oligosaccharides. This mutant, designated AS15-1, incorporates 30-fold less glucosamine into an oligosaccharide-lipid fraction than the wild type. A gel filtration analysis has shown that a small amount of oligosaccharide-lipid corresponding to Man5GlcNAc2-lipid is formed in the mutant. This mutant shows temperature sensitivity for both growth and adhesion to substratum, and constitutively secretes several unusual proteins in large amounts.
D000818:Animals; D002999:Clone Cells / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D008055:Lipids / Q000096:biosynthesis / Q000235:genetics*; D009154:Mutation; D009844:Oligosaccharides / Q000096:biosynthesis / Q000235:genetics*; D010053:Ovary; D011506:Proteins / Q000378:metabolism; D013696:Temperature
D008055:Lipids; D009844:Oligosaccharides; D011506:Proteins