Partial amino acid sequences of the heavy and light chains of botulinum neurotoxin type E.
The dichain type E botulinum neurotoxin, a product of nicking the single chain protein by trypsin, is composed of a heavy and light chains. Sequence of the first 13 and 20 N-terminal residues of these two chains were determined. Also, proof is provided here that (i) the light chain of the nicked (dichain) is derived from the N-terminal one-third of the parent single chain neurotoxin, and (ii) molecular events leading to the activation, of the single chain neurotoxin cannot involve tryptic cleavage at or very close to the N-terminal of the single chain protein. The partial amino acid sequence of the light chain of botulinum type E and tetanus neurotoxins show significant similarity between the two clostridial neurotoxins.
D000595:Amino Acid Sequence; D001905:Botulinum Toxins; D008970:Molecular Weight; D010446:Peptide Fragments; D013744:Tetanus Toxin; D014357:Trypsin / Q000378:metabolism
D010446:Peptide Fragments; D013744:Tetanus Toxin; D014357:Trypsin; D001905:Botulinum Toxins