The interaction between human pancreatic carboxylester hydrolase (bile-salt-stimulated lipase of human milk) and lactoferrin.
An interaction between lactoferrin and human pancreatic carboxylester hydrolase (carboxylic-ester hydrolase, EC 3.1.1.1) (of bile-salt-stimulated lipase from human milk) has been demonstrated using partition in an aqueous two-phase system. This binding was strongly increased by the presence of sodium taurocholate, giving an apparent dissociation constant of around 10(-7) M. With this constant, significant binding is expected to occur in the intestine of the newborn being breast-fed between lactoferrin and either the pancreatic carboxylester hydrolase or the milk bile-salt-stimulated lipase. For carboxylester hydrolase, the interaction with lactoferrin meant a 1.4-fold increase in hydrolytic activity against p-nitrophenylacetate and cholesterololeate. For the function of lactoferrin we have not studied the importance of this interaction.
D001647:Bile Acids and Salts / Q000494:pharmacology; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D005260:Female; D006358:Hot Temperature; D006801:Humans; D066298:In Vitro Techniques; D007231:Infant, Newborn; D007413:Intestinal Mucosa / Q000378:metabolism; D007700:Kinetics; D007781:Lactoferrin / Q000378:metabolism* / Q000494:pharmacology; D007782:Lactoglobulins / Q000378:metabolism*; D008049:Lipase / Q000378:metabolism*; D008895:Milk, Human / Q000201:enzymology; D010179:Pancreas / Q000201:enzymology; D011247:Pregnancy; D011485:Protein Binding
D001647:Bile Acids and Salts; D007782:Lactoglobulins; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; D008049:Lipase; D007781:Lactoferrin