Hybridization with human cells corrects the elevated SCE frequency and BrdU hypersensitivity of hamster cell line EM9.
Cells of the Chinese hamster line EM9 exhibit an elevated SCE frequency and a decreased proliferative response in bromodeoxyuridine (BrdU)-containing medium. Here, these phenotypic features were examined experimentally using polyethylene glycol fusion of EM9 and normal human cells. EM9/human hybrids exhibited both normal SCE frequencies and normal proliferative responses in BrdU-containing medium, suggesting that SCE elevation and BrdU hypersensitivity in EM9 have a common molecular basis.
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002871:Chromosome Banding; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology; D007621:Karyotyping; D008214:Lymphocytes; D010053:Ovary; D010641:Phenotype; D012854:Sister Chromatid Exchange
D001973:Bromodeoxyuridine