Inhibition of hyphal development in Trichophyton mentagrophytes arthroconidia by ketoconazole and miconazole.
In the presence of 0.5 mg/l ketoconazole and miconazole, arthroconidia from three isolates of Trichophyton mentagrophytes produced germ tubes, which continued to grow for 12 h before further elongation ceased. Lower concentrations reduced the rate of elongation, but did not halt the process. Higher concentrations had no greater effect than 0.5 mg/l. The inhibitory effect of ketoconazole was independent of time of addition of drug during the first six hours of germination. Ketoconazole inhibition was reversible after 12 h contact, whereas miconazole inhibition was not. Morphological effects of drug treatment were investigated by scanning electron microscopy. In the absence of imidazoles, hyphae were straight and regular; ketoconazole treated hyphae were short, irregular and branching; miconazole treatment produced extensive debris and spores forming within the hyphae were visible.
D004875:Ergosterol / Q000096:biosynthesis; D007654:Ketoconazole / Q000494:pharmacology*; D008825:Miconazole / Q000494:pharmacology*; D014249:Trichophyton / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure
D008825:Miconazole; D007654:Ketoconazole; D004875:Ergosterol