Regional tritium quenching in quantitative autoradiography of the central nervous system.
Direct corrections of regional differential tritium autoabsorption in quantitative autoradiography of the central nervous system are made using chloroform extraction of brain sections from rats receiving tritiated 2-deoxyglucose in vivo. Fifty-two regional quenching coefficients varied from 30% in gray structures to 100% in white structures. These data may be used to correct for regional quenching of tritium emissions in autoradiographic studies of rat brain.
D000042:Absorption; D000818:Animals; D001345:Autoradiography; D001923:Brain Chemistry; D019298:Glucose-6-Phosphate / Q000031:analogs & derivatives*; D005958:Glucosephosphates / Q000032:analysis; D008297:Male; D008433:Mathematics; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium
D005958:Glucosephosphates; D014316:Tritium; C015785:2-deoxyglucose-6-phosphate; D019298:Glucose-6-Phosphate