[Amine inhibition of serum-induced DNA synthesis in 3T3 and 3T6 cell cultures].
Several amines were shown to inhibit growth stimulation in quiescent confluent and sparse cultures of Swiss 3T3 and 3T6 cells by changing for a fresh medium containing 10-20% serum. Proliferation was inhibited by dansylcadaverine (0.1 mM), chloroquine (0.05 mM), 5-methoxytryptamine (0.1 mM), cystamine (0.1 mM), dimethylurea (100 mM), methylurea (100 mM), and in some experiments--by urea (100 mM). The inhibitory activity was not associated with a direct influence of amines on DNA synthesis or thymidine phosphorylation. The findings suggest that amines may influence the process of clustering of growth factor-receptor complexes, or the fusion of plasma membrane and intracellular vesicles containing some components required for cell proliferation.
D000588:Amines / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D004247:DNA / Q000037:antagonists & inhibitors*; D007106:Immune Sera / Q000494:pharmacology*; D051379:Mice; D009132:Muscles / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010766:Phosphorylation; D013936:Thymidine / Q000378:metabolism
D000588:Amines; D007106:Immune Sera; D004247:DNA; D013936:Thymidine
Podavlenie aminami sinteza DNK, indutsirovannogo syvorotkoĭ v kul'ture kletok 3T3 i 3T6.