The free cytoplasmic calcium concentration of tumorigenic and non-tumorigenic human somatic cell hybrids.
The fluorescent indicator of Ca2+ concentration, quin-2, has been used to measure the concentration of free Ca2+ in the cytoplasm of tumorigenic and non-tumorigenic human somatic cell hybrids. The cell hybrids were derived from the fusion of a HeLa derivative (D98 AH2) and normal human fibroblasts. The calcium concentration of the tumorigenic cell lines was 180 +/- 7nM and the level in the non-tumorigenic cells was 136 +/- 6nM. This difference was statistically highly significant (P less than 0.001). Control experiments are reported which show that the level of 3a2+ measured is not influenced by cell density or by the concentration of quin-2-tetra-(acetoxymethyl)ester used in these experiments. The possible implications of this elevated level of cytoplasmic calcium in tumorigenic cells are discussed.
D000634:Aminoquinolines / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002452:Cell Count; D002471:Cell Transformation, Neoplastic; D003593:Cytoplasm / Q000378:metabolism; D005347:Fibroblasts; D005456:Fluorescent Dyes / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells / Q000187:drug effects / Q000378:metabolism*
D000634:Aminoquinolines; D005456:Fluorescent Dyes; C035738:Quin2-acetoxymethyl ester; D002118:Calcium