[Alanine metabolism in the skeletal muscle and plasma in endotoxemia].
Utilizing a 24 hour fasting rabbit (N = 30), we measured free amino acids in the femoral artery and vein and the quadriceps femoris muscle. The endotoxin E. coli 026, 3.0mg/kg (LD100) was injected and free amino acid plasma levels were monitored for 6 hours. Changes in free amino acid plasma levels were variable and marked after endotoxin injection. By 360 min. after endotoxin injection: (a) the rate of increase in free amino acid levels in the femoral artery was 366 mumole/l of alanine, 162 mumole/l of glycine and 85 mumole/l of proline; (b) the rate of increase in free amino acid of the quadriceps femoris muscle was 1376 nmole/g of alanine, 156 nmole/g of glycine and 109 nmole/g of serine; and (c) the femoral arteriovenous difference was -225 nmole/l of alanine, -118 nmole/l of glycine and -77nmole/l of proline. Within 10 min. after endotoxin injection, alanine concentration was higher in the femoral vein. This change in concentration became significant by 60 min. The results show the following: Skeletal muscle appears to be an important source of amino acids for amino acid metabolism during endotoxemia, especially plasma alanine which is closely connected with alanine levels in skeletal muscle.
D000409:Alanine / Q000097:blood / Q000378:metabolism*; D000818:Animals; D005998:Glycine / Q000378:metabolism; D009132:Muscles / Q000378:metabolism*; D011392:Proline / Q000378:metabolism; D011817:Rabbits; D018805:Sepsis / Q000378:metabolism; D012772:Shock, Septic / Q000378:metabolism*
D011392:Proline; D000409:Alanine; D005998:Glycine