Creatine kinase and lactate dehydrogenase in the muscles encountered during median sternotomy and in the myocardium of the cardiac chambers.
The validity of using creatine kinase MB and lactate dehydrogenase serum isoenzymes to confirm the diagnosis of perioperative myocardial infarction in patients who have had cardiac operations has been questioned, since both have been detected in skeletal muscles. Little is known concerning the concentration of either isoenzyme in the muscles routinely encountered during median sternotomy. Since we have previously shown that the dog is an adequate model in which to study creatine kinase and lactate dehydrogenase isoenzymes, eight healthy dogs were placed under general anesthesia and 1 gm blocks were resected from selected muscles (intercostals, rectus abdominis, diaphragm, and sternothyroid) and from the walls of all four cardiac chambers. Each 1 gm block was homogenized individually in Ringer's lactate, centrifuged, and the supernatants were analyzed for total creatine kinase and lactate dehydrogenase activity by spectrophotometry. Isoenzymes were determined by agarose gel electrophoresis. The study shows that the chest wall muscles and atrial myocardium have appreciable quantities of creatine kinase MB. Hence, serum creatine kinase MB bands in the perioperative period can be generated by manipulation of chest wall muscles and the atrial wall as well as by infarction of the ventricular myocardium.
D000818:Animals; D003402:Creatine Kinase / Q000032:analysis*; D004285:Dogs; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D009132:Muscles / Q000201:enzymology*; D009203:Myocardial Infarction / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology*; D013249:Sternum / Q000601:surgery*
D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase