Acute effects of pentobarbital-anaesthesia on bile secretion.
Male Wistar rats were equipped with permanent catheters in the bile duct and the duodenum under ether anaesthesia, at least seven days before the experiments. By this technique, the enterohepatic circulation can be interrupted for bile collection without direct surgical intervention. 14C-Pentobarbital (26.6 mumole/100 g body wt) was injected intraperitoneally immediately before interruption of the enterohepatic circulation (NBD, Non-Bile Diverted) or after eight days of bile diversion (BD, Bile Diverted). In NBD rats, bile flow and biliary bile acid excretion were significantly reduced during the first hour after pentobarbital administration when compared to unanaesthetized controls, but markedly increased thereafter. Pentobarbital treatment slightly decreased biliary bile acid excretion in BD rats, but caused a 60% increase in bile flow. Within four hours 22.3 +/- 0.4% and 26.0 +/- 2.7% of the injected radioactivity was excreted into bile in NBD and BD rats, respectively. The calculated osmotic activity of pentobarbital and its metabolites was 47.8 +/- 5.2 microliter/mumole in NBD rats and 37.8 +/- 1.3 microliter/mumole in BD rats. Consequently, pentobarbital treatment affected the bile acid independent fraction of bile flow (BAIF). The calculated BAIF was 2.68 microliter/min/100 g body wt in unanaesthetized animals, but 4.27 microliter/min/100 g body wt in pentobarbital treated NBD rats. Corresponding values for BD rats were 1.70 and 2.38 microliter/min/100 g body wt. It is concluded that pentobarbital anaesthesia affects bile production in the rat by direct and indirect means. Firstly, pentobarbital and its metabolites are rapidly excreted into bile and exert a significant choleretic effect, thereby increasing the BAIF. Secondly, pentobarbital anaesthesia retards the exhaustion of the intestinal bile acid pool, which leads to secondary changes in the biliary excretion process.
D000758:Anesthesia; D000818:Animals; D001646:Bile / Q000187:drug effects / Q000378:metabolism*; D001647:Bile Acids and Salts / Q000378:metabolism; D002250:Carbon Radioisotopes; D004764:Enterohepatic Circulation; D008297:Male; D010424:Pentobarbital / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D001647:Bile Acids and Salts; D002250:Carbon Radioisotopes; D010424:Pentobarbital