Preparation and characterization of monoclonal antibody specific for Naja nivea cardiotoxin VII1.
Monoclonal antibodies against Naja nivea cardiotoxin VII1 were produced using the hybridoma technique. The antibodies of two clones were found to be identical by an avidity test, isoelectric focusing and immunodiffusion typing assay. The monoclonal antibody was focused at a pH range of 7.4-8.1 and belonged to the mouse sub-class IgG1. A dissociation constant of 0.26 nM demonstrated its high affinity to cardiotoxin. The monoclonal antibody had no effect on cardiotoxin lethality or lysis of red blood cells by the toxin and could therefore be assumed to bind to an antigenic site separate from the active centre.
D000818:Animals; D000911:Antibodies, Monoclonal; D000917:Antibody Formation; D002459:Cell Fusion; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D004179:Cobra Cardiotoxin Proteins / Q000032:analysis*; D004546:Elapid Venoms / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006461:Hemolysis / Q000187:drug effects; D006825:Hybridomas; D007525:Isoelectric Focusing; D007928:Lethal Dose 50; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight
D000911:Antibodies, Monoclonal; D004179:Cobra Cardiotoxin Proteins; D004546:Elapid Venoms