Immunochemical relatedness between secretory phospholipase A2 and intracellular phospholipase A2.
The immunochemical relationship between rat pancreatic phospholipase A2 and rat splenic phospholipase A2 was examined with the use of anti-rat pancreatic phospholipase A2 antibody as a probe. The immunoelectrophoretic patterns showed that the antibody cross-reacted with the splenic enzyme. The immuno-crossreactivity was also shown by counter immunoelectrophoresis. The splenic phospholipase A2, whether it was purified from the cytosolic fraction or the microsomal fraction, formed an immunoprecipitin band with the anti-pancreatic phospholipase A2 antibody. The antibody was shown to inhibit the activity of the pancreatic phospholipase A2 as well as that of the splenic phospholipase A2.
D000818:Animals; D000906:Antibodies / Q000276:immunology; D003378:Counterimmunoelectrophoresis; D003429:Cross Reactions; D010179:Pancreas / Q000201:enzymology*; D010740:Phospholipases / Q000276:immunology*; D010741:Phospholipases A / Q000276:immunology*; D054467:Phospholipases A2; D011817:Rabbits; D051381:Rats; D013154:Spleen / Q000201:enzymology*
D000906:Antibodies; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2