Automated determination of organic sulfur compounds. I. Spectrophotometric measurement of inorganic sulfate by low-pressure exchange chromatography.
A method for determination of inorganic sulfate is described which represents the terminal step of an automated assay of organic sulfur compounds in biological fluids. Nanomole quantities of inorganic sulfate were applied to a barium chloranilate column. Corresponding amounts of chloranilate ion, released as a result of an exchange reaction, were then measured by uv absorption at 313 nm. The reproducibility and sensitivity of the method and a calibration curve are reported.
D000645:Ammonium Sulfate / Q000032:analysis; D001322:Autoanalysis / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D013053:Spectrophotometry; D013431:Sulfates / Q000032:analysis*
D013431:Sulfates; D000645:Ammonium Sulfate