Evidence for erythrocyte-microvesiculation in vivo.
During physiologic aging, erythrocytes (RBC) mainly loose membrane as shown by biochemical investigations. The most likely reason for a such loss would be the pinch off of RBC-microvesicles. The detection of RBC-microvesicles generated in vivo is rendered more difficult because of the relatively small amount of vesiculation processes under physiologic conditions accompanied by the fast phagocytosis of RBC-microvesicles in the reticulo-histiocytic system. The latter has been shown after the intravenous injection of heat-induced RBC-microvesicles. Investigations of spleens resulted in the case of PHZ-treatment, up till now in a clear demonstration of RBC-microvesicles. The result of microvesiculation in the PHZ-model led to the conclusion, that also rigid, naturally aged RBC could pinch off microvesicles in physiologic narrows of the blood flow like in the spleen.
D000818:Animals; D004905:Erythrocyte Aging; D004910:Erythrocyte Membrane / Q000648:ultrastructure; D004912:Erythrocytes / Q000187:drug effects; D008264:Macrophages / Q000502:physiology / Q000648:ultrastructure*; D008854:Microscopy, Electron; D010537:Peritoneum / Q000166:cytology; D010587:Phagocytosis; D010659:Phenylhydrazines / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000166:cytology
D010659:Phenylhydrazines; C030299:phenylhydrazine