A motility test of leukocytes under agar.
A migration test under agar for leukocytes was developed. Leukocytes moved quite a distance under anaerobic Blood Agar Base (blood agar), a Gibco product. Migration on stained and coloured plates was visualized by projection with a profile projector, making the use of a light microscope superfluous. A migration index was defined. Reproducibility was good enough to allow paired comparisons of leukocyte populations subjected to different treatments. Migration was the result of spontaneous and chemotactically directed migration. Cell-labelling complexes as 111In-oxinate and 111In-tropolonate--ligand concentration 3.5 micrograms/mL in the ultimate cell preparation--did not affect leukocyte migration. 111In-pyrithionate (mercapto pyridine-N-oxide) significantly impaired cell motility. The motility test described could be used as retrospective analysis in abscess localization studies using 111In labelled leukocytes.
D000362:Agar; D002464:Cell Migration Inhibition; D002634:Chemotaxis, Leukocyte; D006801:Humans; D007204:Indium; D007962:Leukocytes / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D011868:Radioisotopes
D011868:Radioisotopes; D007204:Indium; D000362:Agar