An improved technique for extracting catecholamines from body fluids.
This paper describes a technique for selectively extracting catecholamines from body fluids prior to quantification by high-performance liquid chromatography with electrochemical detection. The technique is a two-stage process, the first stage involves the extraction of cations from the sample whilst the second stage is a liquid-liquid extraction involving the complexation of the cationic catecholamines with diphenylborate. This technique provides a very specific extraction procedure which results in chromatograms with no interfering compounds, and gives absolute recoveries of 70-80% for noradrenaline (NA), adrenaline (A) and dihydroxybenzylamine (internal standard), with similar relative recoveries of the 3 compounds. The intra-assay coefficients of variation for the measurement of catecholamines in venous plasma taken from resting subjects, are 8-9% for both NA and A, whilst the inter-assay values are 8% for NA and 20% for A.
D000537:Aluminum Oxide; D001826:Body Fluids / Q000032:analysis; D002395:Catecholamines / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods; D004837:Epinephrine / Q000302:isolation & purification; D006801:Humans; D009638:Norepinephrine / Q000302:isolation & purification
D002395:Catecholamines; D000537:Aluminum Oxide; D009638:Norepinephrine; D004837:Epinephrine