The contribution of myofibers and fibrocytes to palmitate oxidation in skeletal muscle.
Myofibers and fibrocytes were separated from a cell suspension of rat m. flexor digitorum brevis. The procedure was successful only when myofibers were intentionally damaged prior to separation. Density gradient centrifugation yielded a fraction containing 82% of fibrocytes and 20% of myofibers and a second fraction with the complementary 18% and 80% of fibrocytes and myofibers, respectively. Since the damaged myofibers had a negligible palmitate oxidation activity, the relative contribution of fibrocytes and myofibers to palmitate oxidation in the parent cell suspension with intact myofibers could be established, and amounted to 13% and 87%, respectively.
D000818:Animals; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D066298:In Vitro Techniques; D008297:Male; D009132:Muscles / Q000166:cytology* / Q000378:metabolism; D010084:Oxidation-Reduction; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D010169:Palmitic Acids; D019308:Palmitic Acid