The effect of different cooling temperatures and immersion fluids on post-burn oedema and survival of the partially scalded hairy mouse ear.
One ear of NMRI hairy mice was first scalded and then immediately immersed in cold water or saline at 8 degrees C, 15 degrees C, 20 degrees C, 25 degrees C or 30 degrees C for a period of 30 min in order to study oedema formation and the therapeutic effect at 4 days. Oedema was determined by wet-dry weight measurements of punch biopsies from the ear and expressed as an increase in tissue water content. The therapeutic effects at 4 days were determined by observing the survival of the ear; the area of necrosis was expressed in per cent of the total area of the ear. Significant oedema was found in all scalded ears. A partial (15 per cent) dry necrosis of the untreated burned ear had developed within 4 days. Cooling in 8 degrees C water or saline for 30 min post-burn significantly reduced oedema in this model as determined 2h post-burn. However, 4 days post-burn all cooled ears showed a 15 per cent necrosis.
D000818:Animals; D002056:Burns / Q000150:complications*; D017679:Cryotherapy; D004423:Ear / Q000378:metabolism / Q000473:pathology; D004487:Edema / Q000209:etiology / Q000517:prevention & control*; D007101:Immersion; D008297:Male; D051379:Mice; D009336:Necrosis; D013997:Time Factors; D014867:Water / Q000378:metabolism
D014867:Water