Identification of yeast 60 S ribosomal proteins crosslinked to rRNA by 2-iminothiolane.
Saccharomyces carlsbergensis 60 S ribosomal subunits were treated with the hetero-bifunctional crosslinking agent 2-iminothiolane and then subjected to mild UV irradiation to introduce protein-rRNA crosslinks. The major crosslinked products were identified as proteins L2, L3, L5, L19 and L23 of which L5 was found to be crosslinked at a 3-5-fold higher efficiency than the other four. Several additional proteins were cross-linked to a detectable but much lower extent.
D003432:Cross-Linking Reagents / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000378:metabolism; D007096:Imidoesters / Q000494:pharmacology*; D012331:RNA, Fungal / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism* / Q000528:radiation effects; D012269:Ribosomal Proteins / Q000378:metabolism* / Q000528:radiation effects; D012440:Saccharomyces / Q000032:analysis*; D014466:Ultraviolet Rays
D003432:Cross-Linking Reagents; D005656:Fungal Proteins; D007096:Imidoesters; D012331:RNA, Fungal; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; C005905:methyl 4-mercaptobutyrimidate