Human beta-interferon gene expression is regulated by an inducible enhancer element.
We have localized the regulatory sequence required for viral or poly(I)-poly(C) activation of human beta-interferon gene expression to a region located between -37 and -77 from the mRNA cap site. This sequence has the characteristics of an inducible enhancer element: it can act upstream or downstream of the beta-interferon gene regardless of its orientation, and at distances up to approximately 1 kilobase from its normal location. Moreover, this element can confer inducibility on a heterologous promoter. Further analysis has identified a minimal regulatory element of 14 base pairs within this enhancer. Sequences closely related to this element are present five times within the 5'-flanking regions of both the alpha- and beta-interferon genes. The number of these minimal regulatory elements required for maximal beta-interferon gene expression appears to differ in different cell lines.
D000818:Animals; D002460:Cell Line; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D005809:Genes, Regulator; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D011070:Poly I-C / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D013937:Thymidine Kinase / Q000235:genetics; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D007370:Interferon Type I; D013937:Thymidine Kinase; D011070:Poly I-C