Morphology of two pairs of identified peptidergic neurons in the buccal ganglia of the mollusc Tritonia diomedea.
The morphology of two pairs of identified peptidergic neurons (B11 and B12) located in the buccal ganglia of Tritonia diomedea was described. Both pairs of neurons contained a large quantity of a small cardioactive peptide (SCP) in their somata. One of the pairs (B11), the large dorsal white cells, contained ACh in their somata along with SCP. Both pairs of cells appeared white in live preparations under epi-illumination. Each B11 and B12 was a unipolar neuron and sent its major axonal branch through the ipsilateral gastro-esophageal nerve to the gut. In addition, B12 sent a small branch to the contralateral buccal ganglion. A characteristic feature of both neuron pairs was their vesicular content. Three types of vesicles were observed in B11. Vesicles with electron-lucent core (LCV) and electron-dense core (DCV) were found in the somata. The axon hillock and the beginning of axon contained vesicles with variable electron dense core (VDCV) in addition to LCV and DCV. The ratio of DCV: LCV: VDCV changed from 5:95:0 for the perinuclear cytoplasm to 8:55:37 for the beginning of axon. The average maximum diameters were 97 +/- 23 nm for DCV, 103 +/- 32 nm for LCV and 106 +/- 29 nm for VDCV. B12 somata contained DCV (average maximum diameter 100 +/- 26 nm), LCV (109 +/- 23 nm) and elliptical vesicles with eccentric electron-opaque core (115 +/- 20 nm).
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D001369:Axons / Q000648:ultrastructure; D004947:Esophagus / Q000294:innervation; D005724:Ganglia / Q000166:cytology*; D007447:Invertebrate Hormones / Q000378:metabolism*; D008854:Microscopy, Electron; D008974:Mollusca; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000145:classification / Q000648:ultrastructure; D009479:Neuropeptides; D009842:Oligopeptides / Q000378:metabolism*; D013270:Stomach / Q000294:innervation; D013572:Synaptic Vesicles / Q000648:ultrastructure
D007447:Invertebrate Hormones; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D009842:Oligopeptides; C041307:small cardioactive peptide B; D000109:Acetylcholine