Repair of indirectly induced DNA damage in human skin fibroblasts treated with N-hydroxy-2-naphthylamine.
The DNA lesions induced by active oxygen species generated from N-hydroxy-2-naphthylamine were quantitated by the alkaline elution technique as single-strand breaks using cultured human-skin fibroblasts. When cells were treated at 20 degrees C for 2 h with 0-25 microM carcinogen, the lesions increased biphasically with the concentration; the increase was slight below 10 microM while it was much larger and dose-dependent above this concentration. The dose response was similar for normal and xeroderma pigmentosum fibroblasts of complementation group A. There was no difference in the repair rate of single-strand breaks formed in these fibroblasts. The rates of repair of single strand breaks induced by N-hydroxy-2-naphthylamine and hydrogen peroxide were similar but slower than that of the repair of gamma-ray-induced single-strand breaks.
D015081:2-Naphthylamine / Q000031:analogs & derivatives / Q000494:pharmacology*; D002478:Cells, Cultured; D004260:DNA Repair / Q000187:drug effects*; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D009281:Naphthalenes / Q000494:pharmacology*; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D014983:Xeroderma Pigmentosum / Q000235:genetics
D004277:DNA, Single-Stranded; D009281:Naphthalenes; D015081:2-Naphthylamine; C019424:N-hydroxy-2-naphthylamine