Lack of binding of glyceraldehyde-3-phosphate dehydrogenase to erythrocyte membranes under in vivo conditions.
A filtration method is described for separating membrane-free cytoplasm from concentrated erythrocyte haemolysates. The method has been used to assess glyceraldehyde-3-phosphate dehydrogenase binding to erythrocyte membranes. The relative amounts of glyceraldehyde-3-phosphate dehydrogenase and lactate dehydrogenase in the cytoplasm (either oxygenated or deoxygenated) indicate there is no detectable binding of glyceraldehyde-3-phosphate dehydrogenase to the membranes under physiological conditions.
D000332:Aerobiosis; D000693:Anaerobiosis; D004910:Erythrocyte Membrane / Q000378:metabolism*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000097:blood*; D006461:Hemolysis; D006801:Humans; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000097:blood; D011485:Protein Binding
D007770:L-Lactate Dehydrogenase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases