Lesions produced by Clostridium butyricum strain CB 1002 in ligated intestinal loops in guinea pigs.
Heated spores (80 degrees C, 10 min) of Clostridium butyricum strain CB 1002 isolated from a fatal case of necrotising enterocolitis in a human neonate were inoculated into ligated intestinal loops prepared in young conventional guinea pigs. Necropsy findings 18 h later included congestion, patchy haemorrhage of the intestinal mucosa and bacteraemia. No abnormalities were observed in control loops given inocula of inactivated spores (heated at 100 degrees C for 10 min) or TYG 6 medium. The results suggest that vascular lesions are produced by C. butyricum in the intestine of young conventional guinea pigs.
D000818:Animals; D003013:Clostridium / Q000302:isolation & purification / Q000472:pathogenicity*; D004761:Enterocolitis, Pseudomembranous / Q000382:microbiology; D006168:Guinea Pigs; D006801:Humans; D007231:Infant, Newborn; D007413:Intestinal Mucosa / Q000473:pathology; D007422:Intestines / Q000382:microbiology* / Q000473:pathology; D008026:Ligation; D008297:Male; D018805:Sepsis / Q000382:microbiology