Determination of the enantiomers of indoprofen in blood plasma by high-performance liquid chromatography after rapid derivatization by means of ethyl chloroformate.
A rapid method for the determination of (+)- and (-)-indoprofen in blood plasma has been developed and applied to pharmacokinetic work. By means of ethyl chloroformate, indoprofen is coupled to leucinamide, a reaction which is complete in less than 3 min at room temperature. The diastereoisomeric derivatives are then separated by reversed-phase high-performance liquid chromatography. In two surgical patients given racemic indoprofen intravenously, the pharmacologically active (+)-enantiomer had a lower clearance and a lower distribution volume than the (-)-enantiomer, which gave it a slightly longer half-life.
D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005561:Formates; D005563:Formic Acid Esters; D006801:Humans; D007216:Indoprofen / Q000097:blood*; D007700:Kinetics; D008297:Male; D013058:Mass Spectrometry; D010666:Phenylpropionates / Q000097:blood*; D013237:Stereoisomerism
D005561:Formates; D005563:Formic Acid Esters; D010666:Phenylpropionates; C007658:ethyl chloroformate; D007216:Indoprofen