Chronic dietary choline modulates synaptic plasticity in the cerebellar glomeruli of aging mice.
A morphometric investigation was carried out on ethanolic phosphotungstic acid (E-PTA) stained synaptic junctions in the cerebellar glomeruli of adult, old, old choline-deficient and old choline-supplemented mice. Numerical (Nv) and surface (Sv) density as well as average length (L) of the synapses were calculated on 100 pictures per group. A significant reduction of Nv and Sv, as well as an increase of L was found during aging. Choline deficient animals did not show any change as compared to old animals of the same age. In choline supplemented mice Nv and Sv were significantly increased and L significantly decreased, respectively, as compared to old control littermates. No difference was found between adult and choline supplemented mice. In the cerebellar glomeruli only a small fraction of fibers are cholinergic, therefore the present findings support the idea that dietary choline can influence systems other than cholinergic. The possible role of choline supplementation in the modulation of synaptic plasticity via the synthesis and/or turnover of neuronal membrane choline phospholipids, is discussed.
D000818:Animals; D002452:Cell Count; D002531:Cerebellum / Q000166:cytology* / Q000187:drug effects; D002794:Choline / Q000008:administration & dosage*; D004032:Diet; D051379:Mice; D008810:Mice, Inbred C57BL; D013569:Synapses / Q000166:cytology / Q000187:drug effects*
D002794:Choline