A coupled amidolytic assay for thromboplastin (tissue factor) using a fluogenic substrate: its application to monkey leukocyte tissue factor.
A sensitive and quantitative amidolytic assay for thromboplastin (tissue factor) coupled to thrombin formation was established. A fluogenic peptide substrate, Boc-Val-Pro-Arg-MCA, was found to be suitable for the coupled amidolytic assay. The amidolytic assay was applied to measure TF activity of endotoxin-stimulated mononuclear leukocytes and monocytes. The amidolytic assay showed good correlation of 0.97 with the currently used clotting assay upon measuring TF activity of the cellular samples.
D000818:Animals; D001780:Blood Coagulation Tests; D003374:Coumarins; D005456:Fluorescent Dyes; D007962:Leukocytes / Q000032:analysis*; D008251:Macaca; D009842:Oligopeptides; D013050:Spectrometry, Fluorescence; D013925:Thromboplastin / Q000032:analysis*
D003374:Coumarins; D005456:Fluorescent Dyes; D009842:Oligopeptides; C036903:tertiary-butyloxycarbonyl-valyl-prolyl-arginyl-7-amino-4-methylcoumarin; D013925:Thromboplastin