Synthesis of phospholipids containing photoactivatable carbene precursors in the headgroups and their crosslinking with membrane proteins.
Many integral membrane enzymes require for their activity interactions with the polar headgroups of phospholipids, in addition to the hydrophobic interactions within the lipid bilayer. The interactions with the polar headgroups may have preferential or absolute specificity. To study such interactions, phospholipids have been synthesized which carry photoactivable moieties in their headgroups. Three types of phospholipids, PL-I, PL-II and PL-III, were synthesized. The synthetic phospholipids, PL-I and PL-II were able to reconstitute enzymatic activity of the membrane proteins which were studied. Covalent crosslinking between these phospholipids and the membrane proteins was demonstrated after photolysis of the reconstituted phospholipid-protein complexes.
D001436:Bacteriorhodopsins / Q000378:metabolism; D002850:Chromatography, Gel; D003573:Cytochrome b Group / Q000032:analysis; D015786:Cytochromes b5; D008051:Lipid Bilayers / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D008958:Models, Molecular; D010743:Phospholipids / Q000096:biosynthesis*; D010782:Photolysis
D003573:Cytochrome b Group; D008051:Lipid Bilayers; D008565:Membrane Proteins; D010743:Phospholipids; D001436:Bacteriorhodopsins; D015786:Cytochromes b5