Staggered stable isotope administration technique for study of drug distribution.
By infusing intravenously a series of different stable isotope labeled forms of a drug at different times prior to performing a single collection of a body fluid via puncture (or tissue via biopsy), one can obtain the same information about a drug's distribution as would be obtained by infusing a single dose of drug and performing serial collections of fluid (or tissue). Application of this technique of "staggered stable isotope administration" is illustrated with a study of entry of phenobarbital into the cerebrospinal fluid of a dog. Advantages and disadvantages of this technique are discussed.
D000818:Animals; D004285:Dogs; D006207:Half-Life; D007553:Isotope Labeling; D007700:Kinetics; D008297:Male; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D010634:Phenobarbital / Q000097:blood / Q000134:cerebrospinal fluid; D013997:Time Factors
D004364:Pharmaceutical Preparations; D010634:Phenobarbital