A new assay method for lipid peroxides using a methylene blue derivative.
To determine the absolute amount of lipid hydroperoxides in biological materials, a simple and sensitive colorimetric method was newly developed, based on the reaction of lipid hydroperoxides with a leucomethylene blue derivative in the presence of hemoglobin. The amount of methylene blue formed was measured by its absorbance at 666 nm to calculate the amount of lipid hydroperoxides using cumene hydroperoxide as external standard. By this method, lipid hydroperoxide concentrations of less than 7.5 nmol/tube were accurately determined.
D003124:Colorimetry / Q000379:methods; D004396:Coloring Agents; D008054:Lipid Peroxides / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008751:Methylene Blue / Q000031:analogs & derivatives*; D013053:Spectrophotometry / Q000379:methods
D004396:Coloring Agents; D008054:Lipid Peroxides; C011010:hydromethylthionine; D008751:Methylene Blue