Distribution of sialoglycoconjugates on the luminal surface of the endothelial cell in the fenestrated capillaries of the pancreas.
Sialic acid-bearing molecules on the luminal surface of the vascular endothelium in mouse and rat pancreatic capillaries were detected electron microscopically by using a procedure with ferritin hydrazide (FH), after preferential oxidation of sialyl residues with sodium periodate. The distribution of FH on the endothelial surface demonstrated the existence of microdomains with various densities of sialoglycoconjugates oxidizable by sodium periodate and accessible to the tracer. On the plasmalemma proper, FH binding sites were heterogeneously distributed. Their concentration on various microdomains decreased as follows: plasmalemma proper greater than coated pits greater than stomal diaphragms of plasmalemmal vesicles and transendothelial channels, and fenestral diaphragms. The membrane of plasmalemmal vesicles and transendothelial channels was not labeled by FH. Nonspecific binding of FH to the nonoxidized endothelial surface or that oxidized after neuraminidase treatment was relatively low.
D000818:Animals; D002196:Capillaries / Q000032:analysis / Q000648:ultrastructure; D002462:Cell Membrane / Q000032:analysis; D004727:Endothelium / Q000032:analysis / Q000648:ultrastructure; D005293:Ferritins / Q000031:analogs & derivatives; D006651:Histocytochemistry / Q000379:methods; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D019158:N-Acetylneuraminic Acid; D010179:Pancreas / Q000032:analysis / Q000098:blood supply* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D012794:Sialic Acids / Q000032:analysis*; D012795:Sialoglycoproteins / Q000032:analysis
D012794:Sialic Acids; D012795:Sialoglycoproteins; C045101:ferritin hydrazide; D005293:Ferritins; D019158:N-Acetylneuraminic Acid