Metabolism of protein-free lipid emulsion models of chylomicrons in rats.
Emulsions were prepared by ultrasonication of mixtures of triolein, cholesteryl oleate, phosphatidylcholine and cholesterol in aqueous dispersions, then purified by ultracentrifugation. After injection into rats, the metabolism of the artificial, protein-free emulsions was comparable to the metabolism of chylomicrons collected from rat intestinal lymph during the absorption of fat. Like chylomicrons, the emulsion triacylglycerol was removed from the plasma more quickly than emulsion cholesteryl ester. Also like chylomicrons, much more emulsion cholesteryl ester than triacylglycerol appeared in the liver 10 min after injection, and only trace amounts appeared in the spleen. Because the artificial emulsions gained apolipoproteins when incubated with plasma, their metabolism was probably facilitated by the recipient rat plasma apolipoproteins and so, in rats made apolipoprotein-deficient by treatment with estrogen, the removal of emulsions from the plasma was slowed. Removal was also slowed in hyperlipidemic rats fed a high-fat, high-cholesterol diet to expand the plasma pools of the triacylglycerol-rich lipoproteins and remnants. The results indicate that the metabolism of lymph chylomicrons can be modeled by artificial, protein-free lipid emulsions not only in the initial partial hydrolysis by lipoprotein lipase, but also in the delivery of a remnant-like particle to the liver.
D000818:Animals; D002784:Cholesterol; D002788:Cholesterol Esters; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D002914:Chylomicrons / Q000378:metabolism*; D004041:Dietary Fats / Q000008:administration & dosage; D004967:Estrogens / Q000494:pharmacology; D005217:Fat Emulsions, Intravenous / Q000378:metabolism*; D006949:Hyperlipidemias / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008854:Microscopy, Electron; D008954:Models, Biological; D010713:Phosphatidylcholines; D051381:Rats; D014304:Triolein; D014461:Ultracentrifugation
D002788:Cholesterol Esters; D002791:Cholesterol, Dietary; D002914:Chylomicrons; D004041:Dietary Fats; D004967:Estrogens; D005217:Fat Emulsions, Intravenous; D010713:Phosphatidylcholines; D014304:Triolein; C014409:cholesteryl oleate; D002784:Cholesterol