Replication of measles virus in human lymphocytes.
Replication of measles virus was restricted in human peripheral blood mononuclear cells (PBMC). However, in in vitro-infected, unstimulated cells, active synthesis of viral RNA and proteins occurred, while the release of infectious virus could not be detected. Stimulation with PHA caused a productive infection cycle comparable to the lytic infection. Replication of viral RNA was demonstrated in both T and B cells, and in both OKT4+- and OKT8+-depleted T cell subsets. The presence of measles virus RNA was detected in PBMC isolated from measles patients, and the production of the immunoreactive hemagglutinin protein was defective in these cells.
D000328:Adult; D002648:Child; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000378:metabolism / Q000382:microbiology* / Q000648:ultrastructure; D008457:Measles / Q000382:microbiology; D008459:Measles virus / Q000254:growth & development* / Q000378:metabolism; D010835:Phytohemagglutinins / Q000494:pharmacology; D012367:RNA, Viral / Q000096:biosynthesis; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
D010835:Phytohemagglutinins; D012367:RNA, Viral; D014764:Viral Proteins