Concomitant purification of three porcine heart mitochondrial enzymes: citrate synthase, aspartate aminotransferase, and malate dehydrogenase.
The mitochondrial enzymes citrate synthase, malate dehydrogenase, and aspartate aminotransferase were purified to homogeneity from porcine hearts by use of Bio-Rex 70, carboxymethylcellulose CM32, and Affi-Gel blue chromatography. This procedure provides relatively rapid, large-scale preparation of the three enzymes based on their differential binding to commercially available cation-exchange resins followed by a final affinity chromatography step.
D000645:Ammonium Sulfate; D000818:Animals; D001219:Aspartate Aminotransferases / Q000302:isolation & purification*; D005591:Chemical Fractionation; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D002950:Citrate (si)-Synthase / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D008291:Malate Dehydrogenase / Q000302:isolation & purification*; D008929:Mitochondria, Heart / Q000201:enzymology*; D007652:Oxo-Acid-Lyases / Q000302:isolation & purification*; D013552:Swine
D008291:Malate Dehydrogenase; D002950:Citrate (si)-Synthase; D001219:Aspartate Aminotransferases; D007652:Oxo-Acid-Lyases; D000645:Ammonium Sulfate