Light microscope based analysis of three-dimensional structure: applications to the study of Drosophila salivary gland nuclei. I. Data collection and analysis.
Many biological structures of interest are large enough that they may be viewed by light microscope methods, yet they are sufficiently complicated that interpretation of what is seen is quite difficult. The salivary gland nuclei from Dipterans are an example of this. Previous attempts at determining the path of the giant chromosomes in these nuclei have depended on the laborious construction of models by hand. A unified Computer Aided Modelling and Analysis system (CAMA) has been implemented, allowing data collection and analysis of structures visible by light microscopy. This system is extendible to the analysis of electron micrographs of serial sections or of other data consisting of images present in a stack.
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D002875:Chromosomes; D003201:Computers; D004330:Drosophila; D066298:In Vitro Techniques; D008853:Microscopy / Q000379:methods; D008954:Models, Biological; D012469:Salivary Glands / Q000166:cytology