Hemichrome binding to band 3: nucleation of Heinz bodies on the erythrocyte membrane.
Hemichromes, the precursors of red cell Heinz bodies, were prepared by treatment of native hemoglobin with phenylhydrazine, and their interaction with the cytoplasmic surface of the human erythrocyte membrane was studied. Binding of hemichromes to leaky red cell ghosts was found to be biphasic, exhibiting both high-affinity and low-affinity sites. The high-affinity sites were shown to be located on the cytoplasmic domain of band 3, since (i) glyceraldehyde-3-phosphate dehydrogenase, a known ligand of band 3, competes with the hemichromes for their binding sites, (ii) removal of the cytoplasmic domain of band 3 by proteolytic cleavage causes loss of the high-affinity sites, and (iii) the isolated cytoplasmic domain of band 3 interacts tightly with hemichromes, rapidly forming a pH-dependent, water-insoluble copolymer upon mixing in aqueous solution. Since the copolymer of hemichromes with the cytoplasmic domain of band 3 was readily isolatable, a partial characterization of its properties was conducted. The copolymer was shown to be of defined stoichiometry, containing approximately 2.5 hemichrome tetramers (or approximately 5 hemichrome dimers) per band 3 dimer, regardless of the ratio of hemichrome:band 3 in the initial reaction solution. The copolymer was found to be of macroscopic dimensions, generating particles which could be easily visualized without use of a microscope. The coprecipitation was also highly selective for hemichromes, since, in mixed solutions with native hemoglobin, only hemichrome was observed in the isolated pellet. Furthermore, no precipitate was ever observed upon mixing the cytoplasmic domain of band 3 with oxyhemoglobin, deoxyhemoglobin, (carbonmonoxy) hemoglobin, or methemoglobin.(ABSTRACT TRUNCATED AT 250 WORDS)
D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000378:metabolism* / Q000648:ultrastructure; D006366:Heinz Bodies / Q000378:metabolism*; D006420:Hemeproteins / Q000378:metabolism*; D006454:Hemoglobins / Q000302:isolation & purification; D006801:Humans; D007700:Kinetics; D010659:Phenylhydrazines / Q000494:pharmacology; D011485:Protein Binding; D013053:Spectrophotometry
D001457:Anion Exchange Protein 1, Erythrocyte; D006420:Hemeproteins; D006454:Hemoglobins; D010659:Phenylhydrazines; C001332:hemichrome; C030299:phenylhydrazine