Application of derivative spectroscopy to the determination of plasma theophylline in the presence of phenobarbital.
A rapid, simple, and sensitive procedure for the determination of theophylline in plasma using second derivative spectroscopy is described. Second derivative spectra of plasma extracts containing theophylline in concentrations ranging from 2 to 20 micrograms/mL were studied. The method is suitable for the measurement of plasma theophylline in patients treated for cardiorespiratory disorders. Interference from other compounds such as phenobarbital can be avoided.
D055598:Chemical Phenomena; D002621:Chemistry; D004586:Electrophoresis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010634:Phenobarbital / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet; D013806:Theophylline / Q000097:blood*
D013806:Theophylline; D010634:Phenobarbital