Intravesical cytostatics: pH-dependence of antitumour activity.
The effects of pH on the antitumour activity of six cytostatics--cisplatin, doxorubicin, ethoglucid, mitomycin C, thiotepa and VM-26--used in intravesical instillations were tested in vitro. The activity of cytostatics was determined on the Walker 256 carcinosarcoma cell line by using ATP-bioluminescence method. Cytostatics were incubated at different pH's (5.0-7.4) for 2 and 4 h. The activity of most of the cytostatics was not affected by pH, but mitomycin C had somewhat lower activity at pH 5.0 at 4 h and cisplatin at pH 6-7 at 2 and 4 h. Because the pH of normal urine is within the range used in this test, the use of a buffer solution during the intravesical instillation therapy is not necessary. However, according to our clinical experience, buffering is recommended to avoid chemical cystitis.
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000652:urine; D002279:Carcinoma 256, Walker / Q000378:metabolism / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D051381:Rats
D000970:Antineoplastic Agents; D003470:Culture Media; D000255:Adenosine Triphosphate