[Mechanism of the radioprotective effect of cysteamine].
The radioprotective effect of cysteamine combined with the modification of the chromatin state by sodium butyrate has been studied using V-79 and CHEL lines of Chinese hamster cells and HeLa cells. Sodium butyrate enhances the chromatin sensitivity to nucleases and removes the radioprotective effect of cysteamine as measured by the yield of cells with chromosome aberrations. As is indicated by changes in the intensity of fluorescence of the DNA-ethidium bromide complex, measured by laser flow cytometry, the protective agent decreases the binding of the dye with both irradiated and nonirradiated DNA whereas ionizing radiation and sodium butyrate increase thereof. It is concluded that the radioprotective effect of cysteamine depends in its ability to reduce the susceptibility of DNA to nucleases.
D000818:Animals; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002460:Cell Line; D002869:Chromosome Aberrations / Q000187:drug effects* / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D003543:Cysteamine / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism / Q000528:radiation effects*; D005434:Flow Cytometry; D005720:Gamma Rays; D006367:HeLa Cells; D006801:Humans
D002087:Butyrates; D020148:Butyric Acid; D003543:Cysteamine; D004247:DNA
K voprosu o mekhanizme radiozashchitnogo deĭstviia tsisteamina.