Do Toxocara canis larval antigens used in enzyme-linked immunosorbent assay for visceral larva migrans cross-react with AB isohemagglutinins and give false positive results?
A variety of helminth parasites have A and B blood group antigens on their surface. These antigens may cross-react with elevated concentrations of A and B isohemagglutinins in some patients and give false-positive results in the serologic diagnosis of visceral larva migrans caused by T. canis. To clarify this point, serum from patients with visceral larva migrans and elevated T. canis antibody titers as determined by ELISA were absorbed with AB blood cells and retested by ELISA without a demonstrable decline in T. canis antibody titers. Similarly, absorption with T. canis embryonated egg antigens of serum containing elevated levels of anti-A or anti-B isohemagglutinins failed to decrease the isohemagglutinin titer. This indicates that the ELISA using T. canis embryonated egg antigen does not give false positive results with sera containing high concentrations of anti-A or anti-B isohemagglutinins.
D000017:ABO Blood-Group System / Q000276:immunology*; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D006388:Hemagglutinins / Q000276:immunology*; D006801:Humans; D007814:Larva / Q000276:immunology; D007816:Larva Migrans, Visceral / Q000175:diagnosis / Q000276:immunology*; D014119:Toxocara / Q000276:immunology*
D000017:ABO Blood-Group System; D000947:Antigens, Helminth; D006388:Hemagglutinins