Purification and properties of alkaline phosphatase from human polymorphonuclear leukocytes.
A procedure for the purification of alkaline phosphatase from human polymorphonuclear leukocytes is described, involving enzyme solubilisation with Triton X-100 and chromatography on DEAE-Sepharose CL 6B and Cibacron Red F = B-Sepharose 4B. The final enzyme preparation was 244-fold purified and was shown to be capable of hydrolysis of a wide range of phosphorylated substates.
D000469:Alkaline Phosphatase / Q000097:blood* / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000379:methods; D006801:Humans; D007700:Kinetics; D009504:Neutrophils / Q000201:enzymology*; D011485:Protein Binding; D013379:Substrate Specificity
D000469:Alkaline Phosphatase