Preliminary studies on a lectin on the surface of adipocytes.
A lectin-like component on the surface of porcine adipocytes was identified, chiefly by means of the cell adhesion and hemagglutination assays. This component, named porcine adipocyte lectin (PAL), was isolated with Triton X-100 solubilization followed by affinity chromatography. By SDS-polyacrylamide gel electrophoresis, two hands of PAL stained with Coomassie blue were revealed with the apparent molecular weights of 112,000 and 89,000. Moreover, the glycoprotein nature of PAL, was demonstrated by periodic-Schiff reagent staining. PAL was able to recognize maltose, mannose and mannan and glycoproteins with analogous sugar structure in the presence of Ca. By the finding that adipocytes in suspension could be aggregated by adding PAL, the hypothesis was advanced that PAL may be involved in the recognition and adhesion of adipocytes in vivo.
D000273:Adipose Tissue / Q000032:analysis*; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D006386:Hemagglutination Tests; D037102:Lectins / Q000302:isolation & purification* / Q000494:pharmacology; D008565:Membrane Proteins / Q000032:analysis; D013552:Swine
D037102:Lectins; D008565:Membrane Proteins