Cigarette smoke-induced injury of peritoneal mesothelial cells.
Cultured rat peritoneal mesothelial cells have been exposed to plasma obtained from healthy adult male volunteers before and after the smoking of two medium-tar cigarettes. The pre-smoking plasma had little effect on the mesothelial cells as observed by scanning electron microscopy. Post-smoking plasma produced extensive cellular damage evidenced by the production of blebs and microvillus-like structures on the cell surface. Some cells were also found to have a perforated luminal membrane. These results suggest that factors cytotoxic for these cells are present in plasma following exposure of humans to cigarette smoke.
D000328:Adult; D000818:Animals; D001769:Blood; D002470:Cell Survival; D002478:Cells, Cultured; D004727:Endothelium / Q000166:cytology / Q000648:ultrastructure; D004847:Epithelial Cells; D004848:Epithelium / Q000648:ultrastructure; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D009852:Omentum / Q000166:cytology* / Q000648:ultrastructure; D051381:Rats; D012907:Smoking