Production in yeast of hepatitis B surface antigen by R-DNA technology.
Yeast synthesizes the surface antigen protein of Hepatitis B virus when the structural gene is fused to the promoter from the ARG3 gene. Analysis of extracts and total cells shows that the primary translation product can be identified as a poorly antigenic monomer with an estimated molecular weight of 22K. In cell extracts Y-HBsAg is in the form of 20 nm particles which, like serum derived particles, are highly immunogenic in mice and monkeys. Yeast derived surface antigen is thus a viable alternative to the present serum derived HBV vaccines.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002522:Chlorocebus aethiops; D004274:DNA, Recombinant; D004591:Electrophoresis, Polyacrylamide Gel; D006514:Hepatitis B Surface Antigens / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D011863:Radioimmunoassay; D015003:Yeasts / Q000378:metabolism*
D004274:DNA, Recombinant; D006514:Hepatitis B Surface Antigens