Presence of P blood group antigens on human platelets.
Except for ABH antigens, the presence of red cell (RBC) antigens on human platelets has been a source of disagreement among investigators. Because ABH antigens share precursor sequences with P-system saccharides, the authors examined human platelets for evidence of P blood group antigens. Anti-P was directly labeled with 125I and incubated with normal platelets in a one-stage radioimmunoassay (RIA). Alternatively, platelets from donors of known RBC phenotype were incubated with anti-P, anti-P1, or anti-Tja, washed, incubated with FITC-labeled goat anti-human immunoglobulin and evaluated by fluorescence flow cytometry. The results of these assays demonstrate that platelets express P blood group antigens in parallel to the donor's RBCs. The role of these antigens in platelet transfusion is not known.
D001789:Blood Group Antigens / Q000276:immunology*; D001792:Blood Platelets / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007519:Isoantigens / Q000032:analysis*; D010127:P Blood-Group System / Q000276:immunology*; D011863:Radioimmunoassay
D001789:Blood Group Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007519:Isoantigens; D010127:P Blood-Group System