Depression of hepatic microsomal enzyme systems by lentinan in mice.
Studies were performed to determine the effects of an immunopotentiating agent, lentinan, on the hepatic drug-metabolizing enzymes in mice. Lentinan was injected twice a day for two days, and the enzyme activities were determined 12 hr after the last injection of lentinan. A lentinan dose of over 0.25 mg/kg was required to cause a significant decrease (20-40%) in the hepatic microsomal aminopyrine N-demethylase and aniline hydroxylase activities. The loss of drug-metabolizing activity by the treatment with lentinan agreed with the loss of cytochrome P-450 content in many cases. Strain and substrate differences concerning the effect of lentinan on the metabolism of drug were also observed. That is to say, the loss of cytochrome P-450 content by the treatment with lentinan was observed in the ddY, C57BL/6 and BDF1 strain mice, but was not observed in the DBA/2, C3H/He and C57BL/10 strain mice. The decrease in the activities of 7-ethoxycoumarin O-deethylase and biphenyl 2-hydroxylase by the treatment with lentinan was considerably less than that of aminopyrine N-demethylase and aniline hydroxylase in ddY mice.
D000633:Aminopyrine N-Demethylase / Q000378:metabolism; D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D000983:Antipyrine / Q000378:metabolism; D001500:BCG Vaccine / Q000494:pharmacology; D001835:Body Weight / Q000187:drug effects; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003864:Depression, Chemical; D007649:Ketamine / Q000494:pharmacology; D007912:Lentinan / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008862:Microsomes, Liver / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000037:antagonists & inhibitors*; D009929:Organ Size / Q000187:drug effects; D010424:Pentobarbital / Q000494:pharmacology; D011134:Polysaccharides / Q000494:pharmacology*; D012890:Sleep / Q000187:drug effects; D013045:Species Specificity; D013997:Time Factors
D000814:Aniline Compounds; D001500:BCG Vaccine; D011134:Polysaccharides; D007912:Lentinan; D007649:Ketamine; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D000633:Aminopyrine N-Demethylase; D010424:Pentobarbital; D000983:Antipyrine