Determination of the antibiotic fludalanine in plasma and urine by high-performance liquid chromatography using a packed-bed, post-column reactor with o-phthalaldehyde and 2-mercaptoethanol.
Fludalanine is a novel anti-bacterial agent active against gram-negative and gram-positive bacteria. A high-performance liquid chromatographic assay has been developed using ion-pair chromatography to resolve fludalanine and the internal standard 3,3-difluoro-D-alanine from plasma and urine background. The mobile phase contains sodium dodecyl sulfonate and methanol in a phosphate buffer. Fludalanine is derivatized post-column with o-phthalaldehyde via a packed-bed chemical reactor. The adduct is detected fluorometrically. The plasma and urine assays are sensitive to 0.25 and 0.5 micrograms/ml, respectively.
D000409:Alanine / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008623:Mercaptoethanol; D009764:o-Phthalaldehyde
C007742:3-fluoroalanine; D008623:Mercaptoethanol; D009764:o-Phthalaldehyde; D000409:Alanine