Formation of phosphatidylethanol in frozen kidneys from ethanol-treated rats.
We recently identified phosphatidylethanol (Pet) in tissues from ethanol-treated rats. Since phosphatidyl esters are formed artefactually during freezing in plants we wanted to examine if PE was elevated during freezing in animal tissues. Rats were treated with 3 g/kg of ethanol, killed after 3 h and PE was isolated from kidneys at once or after storage at 0, -5, -10, -15, -20 and -80 degrees C for 7 days. Kidneys analyzed at once or after storage at -80 degrees C had Pet equivalent to 0.02 mumol Pet/g. Storage at -10 degrees C and -15 degrees C resulted in increases of Pet to 1.5 mumol Pet/g and 1.2 mumol Pet/g, respectively. Thus, Pet is artefactually elevated during storage of tissues from ethanol-treated rats at lower freezing temperatures, reflecting considerable changes in composition of acidic phospholipids.
D000818:Animals; D002250:Carbon Radioisotopes; D000431:Ethanol / Q000494:pharmacology*; D005038:Ethylnitrosourea / Q000378:metabolism; D005260:Female; D005615:Freezing; D008401:Gas Chromatography-Mass Spectrometry; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D051381:Rats; D011919:Rats, Inbred Strains
D002250:Carbon Radioisotopes; D000431:Ethanol; D005038:Ethylnitrosourea