Dermorphin-like immunoreactivity in guinea pig and rat stomach.
Dermorphin is an opioid peptide containing one D-amino acid isolated from amphibian skin. We examined the presence of dermorphin-like immunoreactivity (DMP-IR) in mammalian brain and tissues using an antiserum developed in our own laboratory which works at a dilution of 1:120,000 for Radioimmunoassay (RIA) and can detect 2.1 pg of dermorphin. We were unable to find DMP-IR in guinea pig, rat and toad brain and rat and toad spinal cord either extracted with methanol or with HCl. The guinea pig and rat stomach contained significant amounts of DMP-IR (228.9 pg/mg protein and 97.5 pg/mg protein respectively) when extracted with HCl but not with methanol. The DMP-IR we detected in the stomach is not dermorphin per se. A single peak of DMP-IR was found on Sephadex G-25 gel filtration. When re-chromatographed on reverse phase HPLC, this peak of DMP-IR gave rise to three peaks of DMP-IR.
D000818:Animals; D002490:Central Nervous System / Q000032:analysis*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004064:Digestive System / Q000032:analysis*; D004723:Endorphins / Q000032:analysis*; D006168:Guinea Pigs; D009842:Oligopeptides / Q000032:analysis*; D018847:Opioid Peptides; D009928:Organ Specificity; D011863:Radioimmunoassay; D051381:Rats; D014551:Urinary Tract / Q000032:analysis*
D004723:Endorphins; D009842:Oligopeptides; D018847:Opioid Peptides; C030058:dermorphin