Formation of radiation-induced cross-links between thymine and tyrosine: possible model for cross-linking of DNA and proteins by ionizing radiation.
A model for radiation-induced cross-linking of DNA and proteins has been developed. It is based on initial formation of free radicals on a DNA base, i.e., thymine, and on an amino acid, i.e., tyrosine. It was demonstrated that interaction of these radicals is highly favored as measured by their kinetics and the cross-linked products. The gas chromatography-mass spectrometry methodology used for the identification of the thymine-tyrosine cross-links is suggested as an experimental approach in the measurements of biological cross-links.
D002849:Chromatography, Gas; D004247:DNA / Q000528:radiation effects*; D008401:Gas Chromatography-Mass Spectrometry; D008954:Models, Biological; D011506:Proteins / Q000528:radiation effects*; D013936:Thymidine / Q000528:radiation effects; D013938:Thymidine Monophosphate / Q000528:radiation effects; D013941:Thymine / Q000528:radiation effects*; D014443:Tyrosine / Q000528:radiation effects*; D014498:Uracil / Q000528:radiation effects
D011506:Proteins; D013938:Thymidine Monophosphate; D014443:Tyrosine; D014498:Uracil; D004247:DNA; D013941:Thymine; D013936:Thymidine