An Anolis skin melanophore assay suitable for photoaffinity labeling studies with alpha-MSH.
Visual determination of MSH-induced pigment migration in melanophores of small pieces of Anolis carolinensis skin is standardized by first measuring photoelectrometrically the change in reflection/transmission of the whole dorsal skin in response to different hormone concentrations. This method allows the rapid and precise recording of time-response curves after photo-affinity labeling of MSH receptors or of dose-response curves of large series of synthetic compounds.
D000345:Affinity Labels; D000818:Animals; D001681:Biological Assay; D008116:Lizards; D009074:Melanocyte-Stimulating Hormones / Q000032:analysis* / Q000378:metabolism; D008547:Melanophores / Q000378:metabolism*; D010777:Photochemistry; D012880:Skin Pigmentation
D000345:Affinity Labels; D009074:Melanocyte-Stimulating Hormones