Altered membrane sialoglycoproteins in human erythrocytes lacking the Gerbich blood group antigens.
The sialoglycoproteins (glycophorins) in human red cell membranes of rare individuals lacking totally (Ge-1,-2,-3 phenotype) or partially (Ge-1,-2,3 phenotype) the Gerbich (Ge) blood group antigens and two Ge-1,-2,-3 heterozygotes were studied by dodecylsulfate polyacrylamide gel electrophoretic techniques. Two sialoglycoproteins (components D and E) were not detectable in the membranes from the homozygotes and found to be decreased by about 50% in those from the heterozygotes. Ge--1,-2,-3 and Ge-1,-2,3 cells were found to contain a 'new' component (mol. masses about 29 and 30 kDa, respectively) possibly representing a D/E hybrid molecule. This sialoglycoprotein was not detectable in membranes from the Ge-1,-2,-3 heterozygotes, suggesting that the Ge-1,-2,-3 phenotype may be caused by at least two different alleles at the Ge blood group antigen locus. Hemagglutination or hemagglutination inhibition tests involving anti-Ge 1,2,3 and -Ge 1,2 as well as native and enzyme-treated normal red cells (phenotype Ge 1,2,3) or membrane and sialoglycoprotein fractions from normal erythrocytes indicate that the receptors of these sera are located within the glycosylated domain(s) of the D and/or E sialoglycoprotein(s). Our data suggest that the Ge locus encodes the polypeptide sequences of the D and E sialoglycoproteins.
D001789:Blood Group Antigens; D003720:Densitometry; D004586:Electrophoresis; D004612:Elliptocytosis, Hereditary / Q000097:blood; D004910:Erythrocyte Membrane / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007519:Isoantigens / Q000032:analysis*; D012394:Rosaniline Dyes; D012795:Sialoglycoproteins / Q000097:blood*; D012967:Sodium Dodecyl Sulfate
D001789:Blood Group Antigens; D007519:Isoantigens; D012394:Rosaniline Dyes; D012795:Sialoglycoproteins; D012967:Sodium Dodecyl Sulfate; C024757:Coomassie brilliant blue R