Effect of brief vascular perfusion on the ultrastructure and activity of mitochondria isolated from the rat heart.
Mitochondria isolated from heart tissue after a 1-min perfusion with Hanks medium were found to have significantly lower rates of State-3 respiration and respiratory control ratios compared to mitochondria isolated from non-perfused hearts. Examination of the mitochondrial preparations by electron microscopy revealed that a large proportion of the mitochondria isolated from perfused heart tissue were swollen and broken compared to mitochondria from non-perfused hearts.
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D003326:Coronary Circulation; D003470:Culture Media; D008297:Male; D008929:Mitochondria, Heart / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure*; D010101:Oxygen Consumption; D010477:Perfusion; D051381:Rats; D011916:Rats, Inbred F344
D003470:Culture Media; D000255:Adenosine Triphosphate