Unusual frequencies of certain alternating purine-pyrimidine runs in natural DNA sequences: relation to Z-DNA.
Prokaryotic, eukaryotic and mitochondrial DNA sequences of total Length 300 000 nucleotides have been analyzed to find out whether stretches of alternating purines and pyrimidines are unusual in terms of occurrence, composition and base sequence. Alternating runs longer than 5 nucleotides are significantly under-represented in the natural sequences as compared to random ones. Octanucleotides are the most deficient, occurring at only 60% of the frequency expected in random sequences. An unexpectedly high proportion of these octamers consists of alternating tetramers with the repeat structure (PuPyPuPy)2 or (PyPuPyPu)2. DNA stretches containing such sequences can potentially form a S1 nuclease sensitive slippage (staggered loop) structure, which might serve as a locally unstacked intermediate in the B- to Z-DNA conformational transition.
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D002417:Cattle; D002645:Chickens; D004247:DNA; D004269:DNA, Bacterial; D004272:DNA, Mitochondrial; D004279:DNA, Viral; D004330:Drosophila; D006801:Humans; D051379:Mice; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides; D011687:Purines; D011743:Pyrimidines; D012091:Repetitive Sequences, Nucleic Acid
D004269:DNA, Bacterial; D004272:DNA, Mitochondrial; D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D011687:Purines; D011743:Pyrimidines; D004247:DNA