The effects of treatment with human beta-interferon on the stimulation of thymidine uptake and DNA synthesis by colchicine in human fibroblasts.
The marked inhibition by beta-interferon (IFN) of colchicine-induced incorporation of [3H]-thymidine into DNA of human fetal lung fibroblasts reflects inhibition of uptake of labeled precursor, rather than an effect on DNA synthesis per se. The percent of cells in S phase as measured with flow cytometry was unchanged by a concentration of IFN that reduced the uptake of labeled thymidine by 50% at 30 h after treatment.
D002453:Cell Cycle / Q000187:drug effects; D003078:Colchicine / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007370:Interferon Type I / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism*; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D007370:Interferon Type I; D014316:Tritium; D004247:DNA; D003078:Colchicine; D013936:Thymidine; D014529:Uridine