Improved recovery of fecal coliforms from the Ottawa River by membrane filters in the presence of food debris.
In the absence of food debris, Sartorius and Millipore HA filters recovered substantially fewer fecal coliforms from Ottawa River water than did Millipore HC. On addition of a small quantity of sterile blended carrot to water samples, recovery by the poorer filters equalled that on Millipore HC. Scanning electron microscopy revealed bacteria sheltered in crevices formed by carrot fibres and thus protected from the normal stresses of exposure. Addition of carrot debris (e.g., 0.03 g carrot to 100 mL of sample) thus provides a convenient and inexpensive means of reducing variations in fecal coliform recovery between brands of membrane filters.
D002170:Canada; D004755:Enterobacteriaceae / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology; D005374:Filtration / Q000295:instrumentation; D005502:Food; D008722:Methods; D014871:Water Microbiology