Amino acid sequence of chicken gizzard beta-tropomyosin. Comparison of the chicken gizzard, rabbit skeletal, and equine platelet tropomyosins.
Chicken gizzard beta-tropomyosin has the same chain length (284 residues) as other muscle tropomyosins, and is most closely related to the beta component of rabbit skeletal muscle. The majority of the amino acid substitutions are restricted to two regions of the structure, residues 185-216 and 258-284. The altered sequences at the COOH-terminal ends (residue 258-284) of the two gizzard components are very similar to each other and to those in platelet tropomyosin and can be correlated with the reduced affinity of interaction of all three tropomyosins with skeletal troponin T and its T1 fragment. The virtually identical NH2-terminal sequences of all four muscle tropomyosin chains indicates that the gizzard proteins' greater ability to polymerize head-to-tail is due to the sequence changes at its COOH terminus. On the other hand, the weaker head-to-tail aggregation of the platelet protein must be due to its NH2-terminal sequence alterations. Examination of the distribution of amino acids and the frequency of their substitution in the a to g positions of the repeating pseudoheptapeptide for all five tropomyosin sequences (four muscle and one platelet) emphasizes the importance of Glu residues at position e. Examination of those features of the muscle sequences implicated in the stabilization of their coiled-coil structures and in their interactions with F-actin suggest only marginal differences among them, with the possible exception of the chicken gizzard gamma component.
D000595:Amino Acid Sequence; D000818:Animals; D001792:Blood Platelets / Q000032:analysis*; D002645:Chickens; D003488:Cyanogen Bromide; D005895:Gizzard, Avian / Q000032:analysis*; D006736:Horses; D009130:Muscle, Smooth / Q000032:analysis*; D009132:Muscles / Q000032:analysis*; D009928:Organ Specificity; D011817:Rabbits; D013045:Species Specificity; D014335:Tropomyosin; D014357:Trypsin
D014335:Tropomyosin; D014357:Trypsin; D003488:Cyanogen Bromide