Carbohydrate metabolism of malarial parasites--I. Metabolism of lactate in Plasmodium knowlesi infected monkey erythrocytes.
Lactate metabolism by Plasmodium knowlesi infected erythrocytes was examined after careful removal of leucocytes from cell preparations. Infected cells were able to metabolize glucose, pyruvate and lactate. Respiration of infected erythrocytes was maximally stimulated by lactate and to a lesser degree by pyruvate and glucose. Respiration of infected cells was insensitive to stimulation by succinate or glutamate or inhibition by malonate. Mepacrine was found to be a potent respiratory inhibitor. Chromatographic analysis of end products of lactate metabolism showed incorporation of carbon from [2-C14]lactate into phosphoenol pyruvate, 3-phosphoglycerate and malate. Experimental data failed to provide evidence for the presence of a functional citric acid cycle activity in infected cells.
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects / Q000502:physiology*; D005947:Glucose / Q000378:metabolism; D007773:Lactates / Q000378:metabolism*; D008253:Macaca mulatta; D008297:Male; D010101:Oxygen Consumption; D010961:Plasmodium / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism
D000962:Antimalarials; D007773:Lactates; D011773:Pyruvates; D005947:Glucose