Natural killer (NK) cells are present in mice with severe combined immunodeficiency (scid).
Spleen cells from C.B- 17 scid mice with severe combined immunodeficiency disease exhibit natural killer cell (NK) activity against YAC lymphoma targets in a standard 4-hr 51Cr release assay. The cytolytic activity is demonstrable only at high effector to target ratios but can be augmented at least sevenfold by the interferon inducer poly I:C. The pattern of target lysis is specific, because splenocytes from poly I:C-primed C.B-17 scid mice lyse NK-sensitive YAC cells and not the insensitive P815 mastocytoma. The presence of several NK-associated antigens on C.B-17 scid splenocytes was tested by pretreating cells with the appropriate antiserum plus complement before testing for NK activity. The results indicate that a proportion of NK effectors in C.B-17 scid mice bear surface NK 2.1 and Asialo GM1 but are negative for Thy-1.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D003602:Cytotoxicity, Immunologic; D005677:G(M1) Ganglioside; D006028:Glycosphingolipids / Q000276:immunology; D007153:Immunologic Deficiency Syndromes / Q000276:immunology*; D007518:Isoantibodies / Q000502:physiology; D007694:Killer Cells, Natural / Q000276:immunology*; D008223:Lymphoma / Q000276:immunology; D051379:Mice; D008817:Mice, Mutant Strains / Q000276:immunology*; D010641:Phenotype; D013154:Spleen / Q000166:cytology
D000911:Antibodies, Monoclonal; D006028:Glycosphingolipids; D007518:Isoantibodies; C035678:anti-Thy antibody; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside