In vitro and in vivo induction of neutrophil and eosinophil chemotactic responses by Schistosoma japonicum cercaria.
High neutrophil and eosinophil chemotactic activities of soluble extract of Schistosoma japonicum cercariae (SjCe-ext) were detected in vitro by using blind-well chemotaxis chambers and Millipore filters in a dose-dependent manner. Low doses (total of 0.25 and 2.5 micrograms) of SjCe-ext induced significant neutrophilic infiltrations in normal and S. japonicum-infected (8 week) guinea pig tissues beginning at 2 hr after the intradermal injection. Eosinophilic responses were observed in the infected animals especially in early phase (2-8 hr post-injection of SjCe-ext). SjCe-ext elicited strong immediate skin reactions (at 30 min) in the infected guinea pigs. Possible roles of these chemotactic activities and hypersensitivity reaction in S. japonicum infection are discussed.
D000818:Animals; D002634:Chemotaxis, Leukocyte; D004306:Dose-Response Relationship, Immunologic; D004804:Eosinophils / Q000502:physiology*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007271:Injections, Intradermal; D009504:Neutrophils / Q000502:physiology*; D012549:Schistosoma japonicum / Q000276:immunology*; D012552:Schistosomiasis / Q000276:immunology; D012867:Skin / Q000276:immunology / Q000469:parasitology; D012908:Snails / Q000469:parasitology