Solutions for preservation of the heart at 0 degrees C.
Solutions developed for cardioplegia and myocardial protection during elective ischemic cardiac arrest have also been used for the storage of heart grafts at 0 degrees C. In this study we show that a solution based upon the principles established in renal preservation experiments gives superior preservation of rabbit hearts at 0 degrees C. The solution contained K+ 25 mmol/L, Mg++ 10 mmol/L, sufficient glucose to prevent fluid uptake during storage (30 mmol/L), and a low concentration of calcium (0.1 mmol/L). The complete omission of calcium was detrimental to function after prolonged storage.
D000818:Animals; D001639:Bicarbonates; D002118:Calcium; D002122:Calcium Chloride; D005615:Freezing; D005947:Glucose; D006321:Heart; D006324:Heart Arrest, Induced; D008274:Magnesium; D009926:Organ Preservation; D011188:Potassium; D011189:Potassium Chloride; D011817:Rabbits; D012965:Sodium Chloride; D013997:Time Factors
D001639:Bicarbonates; C041711:St. Thomas' Hospital cardioplegic solution; D012965:Sodium Chloride; D011189:Potassium Chloride; D008274:Magnesium; D005947:Glucose; D002122:Calcium Chloride; D011188:Potassium; D002118:Calcium