[Density and age of cells in the population of the methanol-assimilating yeast Candida boidinii].
A population of the yeast Candida boidinii growing in the chemostat in a medium with methanol was separated in dextran solutions of different density. The density of cells was found to vary from 1.04 to 1.11 g/cm3, being 1.08 g/cm3 on the average. Changes in the cell density correlated with variations in the content of water and dry matter in the cells and its density. Cell fractions of different density contained cells which mainly belonged to a certain phase in the cell cycle. However, the fractions were not homogeneous. The authors propose a scheme to account for changes in the cell density during the cell cycle. According to this scheme, within the cycle, the density of the cell rises twice (when the cell prepares for the budding and in the budding phase II) and drops down twice (in the budding phases I and III).
D002175:Candida / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D002453:Cell Cycle; D002499:Centrifugation, Density Gradient; D000432:Methanol / Q000378:metabolism; D013997:Time Factors
D000432:Methanol
Plotnost' i vozrast kletok populiatsii metanolusvaivaiushchikh drozhzheĭ Candida boidinii.