Occurrence of met-enkephalin, met-enkephalin-Arg6-Phe7 and met-enkephalin-Arg6-Gly7-Leu8 in gastrin cells of hog antral mucosa.
Region-specific antisera to three enkephalins: met-enkephalin, met-enkephalin-Arg6-Phe7 and met-enkephalin-Arg6-Gly7-Leu8, together with four region specific antisera to progastrin: C-terminal G17 specific, N-terminal G34 specific, cryptic peptides A- and B-specific, were used in immunohistochemical studies of hog antral mucosa. A sub-population (6-10%) of the gastrin-containing endocrine cells (G-cells) was found to react with antisera to met-enkephalin, met-enkephalin-Arg6-Phe7 and met-enkephalin-Arg6-Gly7-Leu8. About 30% of all the enkephalin-containing cells were identified as G-cells. The results indicate that a fraction of G-cells produces both enkephalin-like peptides and gastrin.
D000818:Animals; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives / Q000378:metabolism*; D005753:Gastric Mucosa / Q000166:cytology / Q000378:metabolism*; D005755:Gastrins / Q000378:metabolism*; D006651:Histocytochemistry; D011706:Pyloric Antrum / Q000166:cytology / Q000378:metabolism; D013552:Swine
D005755:Gastrins; D004744:Enkephalin, Methionine; C027511:enkephalin-Met, Arg(6)-Phe(7)-; C033453:enkephalin-Met, Arg(6)-Gly(7)-Leu(8)-