Maturation of Xenopus oocytes is not accompanied by electrode-detectable calcium changes.
Ca2+-sensitive microelectrodes were used to study changes in cytoplasmic free calcium during progesterone-induced meiotic maturation in Xenopus laevis oocytes. In contrast to previous reports, no changes were detected during the maturation process, although a large activation pulse of Ca2+ was seen when in vitro matured eggs were parthenogenetically activated. The resting level of Ca2+ in the oocytes was 0.14 microM +/- 0.05.
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D005260:Female; D008540:Meiosis / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology; D014982:Xenopus laevis / Q000378:metabolism*
D011374:Progesterone; D002118:Calcium