In vitro proliferation and terminal differentiation of quail neural crest cells in a defined culture medium.
We report the formulation of a culture medium, medium MCDB202-21, that supports the in vitro proliferation of quail neural crest cells and their differentiation into melanocytes and adrenergic neuroblasts in the complete absence of serum and chick embryo extract. McKeehan & Ham's medium MCDB 202 was supplemented with hormones, stimulators of metabolism, vitamins, trophic factors, transport molecules, and small molecular nutrients.
D000818:Animals; D002395:Catecholamines / Q000378:metabolism; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D009432:Neural Crest / Q000166:cytology* / Q000378:metabolism; D011784:Quail
D002395:Catecholamines; D003470:Culture Media