Uninterrupted DNA synthesis during S-phase in untransformed diploid hamster fibroblasts.
Untransformed Syrian hamster fibroblasts in exponential growth were exposed to a pulse of [3H]-thymidine for 5 min, followed immediately by bromodeoxyuridine, and serial samples were taken up to 16 h. Preparations were autoradiographed and stained for replication banding. No cell with replication bands was found without significant [3H]-thymidine uptake, although the extent of uptake varied between sub-phases of S. Thus there is no indication of a total cessation of synthesis at any period during S-phase.
D000818:Animals; D002478:Cells, Cultured; D002875:Chromosomes / Q000378:metabolism; D006224:Cricetinae; D004261:DNA Replication; D005347:Fibroblasts; D007399:Interphase; D008647:Mesocricetus; D012093:Replicon