The separation of globin chains by high pressure cation exchange chromatography.
A high performance cation exchange system is described for isolating globin chains. The method is rapid and inexpensive, using total lysate, a 14 min gradient, and a 3 cm column. The running buffers contain 6 M urea to dissociate and unfold the globin, and a NaCl gradient is employed for elution. The procedure provides a convenient way of detecting mutant globins with an intrinsic alteration in their charge.
D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005914:Globins / Q000032:analysis*; D006455:Hemoglobins, Abnormal / Q000032:analysis*; D006801:Humans
D006455:Hemoglobins, Abnormal; D005914:Globins