Human somatotropin: noncovalent interactions of the natural NH2-terminal 1-134 segment with synthetic analogs of the COOH-terminal fragment.
Three analogs of the COOH-terminal fragments of human somatotropin (HGH), namely [Nle170,Ala165,182]-HGH-(150-187), [Nle170,Ala165,182]-HGH-(152-187), and [Nle170,Ala165,182]-HGH-(154-187), have been synthesized by the solid-phase method. The synthetic analogs were complemented with the natural NH2-terminal fragment [Cys(Cam)53]-HGH-(1-134) to form recombinants with HGH activities, as revealed by the rabbit liver membrane receptor binding and the Nb2 lymphoma cell assays.
D000595:Amino Acid Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D013006:Growth Hormone / Q000031:analogs & derivatives / Q000032:analysis*; D006801:Humans; D007525:Isoelectric Focusing; D010446:Peptide Fragments / Q000032:analysis*; D011388:Prolactin / Q000032:analysis; D011869:Radioligand Assay
D010446:Peptide Fragments; D011388:Prolactin; D013006:Growth Hormone