Measurement of cholecystokinin octapeptide using a new specific radioimmunoassay.
A peptide analogue of CCK-8 (Tyroc) which has a tyrosine in place of the amide group in the C-terminal end, has been used both for raising antisera and for iodination. The antisera produced by immunisation with Tyroc are directed towards the N-terminal end of the CCK-8 molecule. The assay system appears totally specific for the CCK-8 sulphated molecule and shows no significant cross-reaction with other molecular forms of CCK, or with the gastrins. The assay can detect changes between adjacent tubes of 0.25 fmol/tube CCK-8 with 95% confidence. The assay is robust, reliable and reproducible and can be used to measure tissue and plasma levels of CCK-8.
D000818:Animals; D000918:Antibody Specificity; D001923:Brain Chemistry; D006801:Humans; D011817:Rabbits; D011863:Radioimmunoassay; D012844:Sincalide / Q000032:analysis* / Q000276:immunology
D012844:Sincalide