Determination of inorganic sulphate in plasma by reversed-phase chromatography using ultraviolet detection and its application to plasma samples of patients receiving different types of haemodialysis.
The determination of sulphate in plasma is described, making use of reversed-phase high-performance liquid chromatography with ultraviolet detection. The concentration of inorganic sulphate determined in plasma of twenty healthy volunteers was 0.307 +/- 0.092 mmol/l (mean +/- S.D.). In one stable chronic dialysis patient the kinetics of plasma sulphate removal were monitored during and after one single pass dialysis. In addition, plasma sulphate concentrations were determined in three stable chronic dialysis patients during a consecutive scheme of two single pass dialyses, five Redy dialyses and three single pass dialyses. As expected, plasma sulphate accumulates in plasma to a high steady-state level under Redy dialysis, whereas during single pass dialysis sulphate is efficiently removed.
D000328:Adult; D000368:Aged; D002852:Chromatography, Ion Exchange / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis; D013056:Spectrophotometry, Ultraviolet; D013431:Sulfates / Q000097:blood*; D013997:Time Factors
D013431:Sulfates