Elemental analysis of individual rat blood platelets by electron probe X-ray microanalysis using a direct quantification method.
The elemental content of individual rat blood platelets and their dense granules was determined by electron probe X-ray microanalysis using a direct quantification method with microdroplets as standards. The quantification procedure was a modification of the 'direct mass' method involving a correction for differences in electron beam intensity in the analysis of standards and specimens. Whole air-dried platelets had a mean magnesium content of 12 X 10(-8) nmol (SE = 1 X 10(-8) nmol, n = 68) and a mean calcium content of 3.2 X 10(-8) nmol (SE = 0.3 X 10(-8) nmol, n = 68). A good correlation was found between the magnesium content and the phosphorus content of the dense granules of the platelets (r = 0.95). There was also good correlation (r = 0.77) between the number of dense granules per platelet and the magnesium content of the platelets.
D000818:Animals; D001792:Blood Platelets / Q000032:analysis*; D002118:Calcium / Q000032:analysis*; D003594:Cytoplasmic Granules / Q000032:analysis*; D004577:Electron Probe Microanalysis; D008274:Magnesium / Q000032:analysis*; D010758:Phosphorus / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D010758:Phosphorus; D008274:Magnesium; D002118:Calcium