Liquid chromatographic determination of the enantiomeric composition of amphetamine and related drugs by diastereomeric derivatization.
A procedure is described for the derivatization of amphetamine and methamphetamine enantiomers with 4-nitrophenylsulfonyl-(S)-prolyl chloride. The resulting diastereomeric amphetamine derivatives were resolved by a reversed-phase procedure. The derivatization yield was maximized in the 95% range using a five-fold molar excess of the chiral derivatizing agent and an approximate 18 to 1 ratio of base (NaHCO3) to total acid generated in the reaction.
D000661:Amphetamine / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D012997:Solvents; D013237:Stereoisomerism
D007202:Indicators and Reagents; D012997:Solvents; D000661:Amphetamine