Functional properties of polymorphonuclear leukocytes accumulated in a skin chamber.
Polymorphonuclear leukocytes harvested from a skin chamber were compared with peripheral blood leukocytes by examining their migration under agarose, their bactericidal capacity and their chemiluminescence. The chamber leukocytes were chemotactically de-activated and their bactericidal capacity reduced. The chemiluminescent response of chamber leukocytes was increased. In response to stimulation with formylmethionyl-leucyl-phenylalanine (first peak), chemiluminescence was far more marked in chamber leukocytes (10-30 times) than in blood leukocytes, whereas in response to stimulation with opsonized zymosan it was only moderately more marked (3-5 times). Exposed to zymosan activated serum, blood leukocytes showed a similar functional modification as leukocytes harvested from a skin chamber, and our findings suggest that the altered function of leukocytes at an inflammatory focus is largely the result of their exposure to chemotactic factors.
D000328:Adult; D001770:Blood Bactericidal Activity; D002470:Cell Survival; D002634:Chemotaxis, Leukocyte; D005260:Female; D006801:Humans; D007249:Inflammation / Q000276:immunology*; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000502:physiology*; D010587:Phagocytosis; D012685:Sepharose; D012867:Skin / Q000276:immunology*
D012685:Sepharose