Enzymatic microdetermination of free fatty acids in plasma of animals using paraoxon to prevent lipolysis.
A simple, sensitive and rapid enzymatic method, manual as well as automated, is described for the determination of free fatty acids in plasma of animals. Spontaneous hydrolysis of esterified fatty acids present in the plasma samples is prevented by adding a specific lipase-esterase inhibitor.
D000818:Animals; D002417:Cattle; D002498:Centrifugation / Q000295:instrumentation; D004285:Dogs; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D008066:Lipolysis / Q000187:drug effects*; D010261:Paraoxon / Q000494:pharmacology*; D011817:Rabbits; D051381:Rats; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods
D005230:Fatty Acids, Nonesterified; D010261:Paraoxon