Bovine serum eliminates rapid nonspecific toxic reactions during bioassay of stored fish for Clostridium botulinum toxin.
When stored fish or some fish products were tested for the presence of Clostridium botulinum toxin, nonspecific toxic reactions in mice often occurred, rendering the bioassay inconclusive. The nonspecific toxic reactions were mediated by the gram-negative microbiota, inherent to the fish, which were the source of lethal, heat-stable endotoxins. The treatment of assay samples with bovine serum eliminated nonspecific reactions through the interaction of constituent serum immunoglobulin M (IgM) with endotoxic material. Removal of IgM from bovine serum through treatment with protein A or concanavalin A resulted in a loss of protective activity.
D000818:Animals; D001681:Biological Assay; D001770:Blood Bactericidal Activity; D001905:Botulinum Toxins / Q000032:analysis*; D002417:Cattle; D003208:Concanavalin A / Q000494:pharmacology; D005396:Fish Products; D005399:Fishes / Q000382:microbiology*; D005516:Food Microbiology; D007075:Immunoglobulin M / Q000276:immunology; D051379:Mice; D013205:Staphylococcal Protein A / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
D007075:Immunoglobulin M; D013205:Staphylococcal Protein A; D003208:Concanavalin A; D014357:Trypsin; D001905:Botulinum Toxins