Organ culture of adult cockroach CNS: ultrastructural and physiological characteristics.
The abdominal nerve cord of adult Periplaneta americana has been maintained in culture for up to 5 weeks. The ultrastructural appearance of in vitro cords resembles that of in vivo cords although some degeneration of glia may occur during the first 2 weeks in culture. The glial cells most affected are those which make up the perineurium. The blood-brain barrier, formed by the perineurium in vivo, breaks down in vitro. Despite this, normal action potentials were recorded from giant axons in 1 month old cultured connectives.
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001369:Axons / Q000502:physiology / Q000648:ultrastructure; D002490:Central Nervous System / Q000502:physiology / Q000648:ultrastructure; D003058:Cockroaches / Q000033:anatomy & histology*; D005724:Ganglia / Q000648:ultrastructure; D007365:Intercellular Junctions / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D009457:Neuroglia / Q000648:ultrastructure; D009924:Organ Culture Techniques; D011188:Potassium / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology
D012964:Sodium; D011188:Potassium