gamma-Hydroxybutyrate uptake by rat brain striatal slices.
gamma-Hydroxybutyrate uptake by rat brain striatal slices was studied. The uptake was saturable with a Km of 702 +/- 107.10(-6) M. gamma-Hydroxybutyrate uptake was sodium dependent and inhibited by the omission of potassium. In addition, the effect of ouabain suggests that the transport is dependent on a cation gradient. Several analogues of gamma-hydroxybutyrate inhibit the transport system. GABA has no significant effect. This energy and cation dependent transport system is in favor of a transmitter or modulator role of gamma-hydroxybutyrate in the rat brain striatum.
D000818:Animals; D003342:Corpus Striatum / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000494:pharmacology; D012978:Sodium Oxybate / Q000031:analogs & derivatives / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014316:Tritium
D006885:Hydroxybutyrates; D014316:Tritium; D012978:Sodium Oxybate; D012964:Sodium; D011188:Potassium