Generation of purpura-producing principle from pneumococcal cell walls.
The in vitro kinetics of muramic acid-alanine bond hydrolysis and pneumococcal purpura-producing principle generation by incubation of Streptococcus pneumoniae cell wall preparations with the bacterial autolysin N-acetylmuramyl-L-alanine amidase were similar. The generated purpura-producing principle preparation had a weight-average molecular weight of ca. 2.6 X 10(7) and possessed the glycan and teichoic acid constituents of the pneumococcal cell wall. The results support the idea that the pneumococcal purpura-producing principle is a high-molecular-weight, glycan-teichoic acid fragment released by hydrolysis of the muramic acid-alanine bonds in the bacterial cell wall.
D000596:Amino Acids / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D002473:Cell Wall / Q000032:analysis; D002794:Choline / Q000032:analysis; D008970:Molecular Weight; D010457:Peptidoglycan / Q000302:isolation & purification*; D010710:Phosphates / Q000032:analysis; D011693:Purpura / Q000382:microbiology*; D013296:Streptococcus pneumoniae / Q000032:analysis* / Q000472:pathogenicity; D013329:Structure-Activity Relationship
D000596:Amino Acids; D002241:Carbohydrates; D010457:Peptidoglycan; D010710:Phosphates; C029323:pneumococcal purpura-producing principle; D002794:Choline