Electrophoretic similarity of the ciliary ganglion survival factors from different tissues and species.
The survival of dissociated ciliary ganglion neurons is promoted by extracts of several different embryonic and adult tissues from two species. The survival-promoting activity in each of these extracts survives exposure to sodium dodecyl sulfate (SDS) and sulfhydryl-reducing agent and can, therefore, be subjected to SDS-polyacrylamide gel electrophoresis. Upon electrophoresis, the survival-promoting activity is recovered in a discrete peak at an apparent molecular weight of approximately 21,800 for all of the tissues examined. These results suggest that a similar molecule in each of these different tissues and species may be responsible for their ability to promote the survival of ciliary ganglion nerve cells in culture.
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002621:Chemistry; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D005724:Ganglia / Q000166:cytology* / Q000187:drug effects*; D006133:Growth Substances / Q000032:analysis; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D009474:Neurons / Q000187:drug effects*; D014020:Tissue Extracts / Q000494:pharmacology*
D006133:Growth Substances; D014020:Tissue Extracts