Viability control and oxygen consumption of isolated hepatocytes from thermally acclimated rainbow trout (Salmo gairdnerii).
Hepatocytes were isolated by collagenase perfusion of the liver from rainbow trout acclimated to 10 and 20 degrees C. The suitability of the stimulation of cellular respiration by succinate as criterion of viability was examined and discussed. Endogenous respiration rates of the hepatocytes were a function of cell size to the power of 0.8. Specific oxygen consumption of the hepatocytes and respiratory control ratios of the mitochondria in situ were independent of acclimation temperature.
D000064:Acclimatization; D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D005260:Female; D007700:Kinetics; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008297:Male; D010101:Oxygen Consumption; D013696:Temperature; D014337:Trout