Effects of storage conditions on bovine carrier erythrocytes.
Bovine carrier RBC containing [14C]sucrose as a marker were stored for 23 days in protein-free media. Cell recovery was highest with a phosphate buffer (pH 7.0) containing 2 mM MgCl2, 5 mM inosine, 5 mM adenosine, and 10 mM glucose. A cell recovery of 60% was observed after 23 days of storage at 4 C. Stored carrier erythrocytes reinjected into cattle had an 18-day half-life, which is less than that for fresh carrier RBC.
D000255:Adenosine Triphosphate / Q000097:blood; D000818:Animals; D001793:Blood Preservation / Q000662:veterinary*; D002021:Buffers / Q000494:pharmacology; D002250:Carbon Radioisotopes; D002417:Cattle / Q000097:blood*; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D005260:Female; D006207:Half-Life; D006454:Hemoglobins / Q000378:metabolism; D013395:Sucrose
D002021:Buffers; D002250:Carbon Radioisotopes; D006454:Hemoglobins; D013395:Sucrose; D000255:Adenosine Triphosphate