Recurrent inhibition of cat phrenic motoneurons.
These experiments were performed to re-examine the presence of a recurrent inhibitory pathway to phrenic motoneurons in cats. Following intracellular staining of 49 phrenic motoneurons with horseradish peroxidase, initial axon collaterals were demonstrated in six axons (12%). Intracellular recording and averaging of the synaptic responses evoked by stimulation of the phrenic roots with an intensity subthreshold for axons of the examined motoneurons revealed (in spinalized cats with dorsal roots cut) inhibitory postsynaptic potentials (i.p.s.p.s) in 11/28 (40%) motoneurons (amplitude, 115 +/- 69 (SD) microV; latency, 3.0 +/- 0.6 msec; duration from 12 to 25 msec). In the vicinity of the phrenic nucleus, Renshaw-like units were extracellularly recorded, which fired high-frequency bursts of action potentials following the stimulation of the phrenic roots (burst duration, 8 to 21 msec). Antidromic activation of the C5 phrenic root reduced the firing probability of fibers in the C6 root. The duration of the reduced firing probability corresponded to the duration of both the i.p.s.p.s and the Renshaw units' firing period. It is concluded that Renshaw inhibition is present in phrenic motoneurons. The inhibitory effects of this recurrent pathway may be greater than judged from the small amplitude of the i.p.s.p.s recorded in individual motoneurons, particularly when the whole phrenic pool is activated and when motoneurons are near threshold. It is suggested that one of the functions of the pathway is to control phrenic motoneurons supplying the dorsal part of the diaphragm through the C6 root when the more ventral part of the diaphragm is strongly activated.
D000818:Animals; D001369:Axons / Q000502:physiology / Q000648:ultrastructure; D002415:Cats; D003712:Dendrites / Q000502:physiology / Q000648:ultrastructure; D008564:Membrane Potentials; D009046:Motor Neurons / Q000502:physiology / Q000648:ultrastructure*; D009433:Neural Inhibition; D009434:Neural Pathways / Q000033:anatomy & histology / Q000502:physiology; D010791:Phrenic Nerve / Q000502:physiology / Q000648:ultrastructure*; D013116:Spinal Cord / Q000166:cytology / Q000502:physiology; D013569:Synapses / Q000502:physiology / Q000648:ultrastructure