Isolation of a cytopathic factor from Mycoplasma hyopneumoniae.
A cytopathic factor was isolated from M. hyopneumoniae VPP11 membranes. This factor had an isoelectric point of 6.2 and was capable of inducing cytopathic effect in MRC-5 human lung fibroblast culture at a concentration of 250 ng of protein per ml. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of this cytopathic factor showed a major band with a molecular weight of approximately 54,000.
D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival; D005347:Fibroblasts; D006801:Humans; D007526:Isoelectric Point; D008168:Lung; D008565:Membrane Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D008970:Molecular Weight; D009174:Mycoplasma / Q000032:analysis*
D001426:Bacterial Proteins; D008565:Membrane Proteins