Quantitative determination of methyltestosterone and methyltestosterone-d3 in serum by gas chromatography-mass spectrometry.
A method for the quantitative estimation of methyltestosterone and methyltestosterone-d3 in biological fluids has been developed using gas chromatography-mass spectrometry-selected-ion monitoring. Methyltestosterone-d6 was used as an internal standard. Methyltestosterone and methyltestosterone-d3 in serum were determined based on the peak height ratios of the molecular ions of methyltestosterone, methyltestosterone-d3 and methyltestosterone-d6. Sensitivity, specificity, precision, accuracy and reproducibility of the present method were demonstrated to be satisfactory for application to pharmacokinetic and bioavailability studies.
D001682:Biological Availability; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007700:Kinetics; D008777:Methyltestosterone / Q000097:blood*
D008777:Methyltestosterone