Interaction between bisantrene and radiation.
The interaction between Bisantrene, a new anthracene derivative, and radiation was studied in vitro. Chinese hamster lung (V-79-171) cells grown as monolayers were exposed to three different concentrations (0.4 mcg/ml, 2 mcg/ml, 10 mcg/ml) of Bisantrene for 120 min. during their exponential growth phase. Immediately after drug exposure, the cells were irradiated with escalating doses of radiation (100-1200 rad). Isoeffect curves (99% cell kill) were calculated using dose-survival curves generated for each modality. Curve I assumed that the agents act by independent mechanisms; Curve II assumed that the agents act by a similar mechanism. The dose combinations of the two modalities producing a 99% kill were plotted against the calculated isoeffect curves. The experimental points were found to lie along the calculated lines. Pre-treatment with Bisantrene prior to radiation resulted in an additive effect, similar to that reported with Adriamycin.
D000818:Animals; D000873:Anthracenes / Q000494:pharmacology; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects*; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D066298:In Vitro Techniques
D000873:Anthracenes; D000903:Antibiotics, Antineoplastic; C031404:bisantrene