An evaluation of ELISA kits for rubella IgG- and IgM-antibodies.
Two ELISA kits for rubella IgG- and IgM-antibodies, respectively, were compared with a haemagglutination inhibition (HAI) test (after ultracentrifugation of the sample if IgM was to be detected). When screening 535 samples from pregnant women, 99.6% agreement was found between the IgG-ELISA and HAI; one true IgM-positive was encountered in this group with the IgM-ELISA. An agreement of 95.5% was found between the IgM-ELISA and HAI after ultracentrifugation. In at least some of the discrepant samples the IgM-ELISA may be assumed to have given the correct answer.
D000914:Antibodies, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation; D005260:Female; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis / Q000517:prevention & control; D012409:Rubella / Q000175:diagnosis* / Q000517:prevention & control
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M