Chromatin degradation in isolated nuclei of normal and transformed baby hamster kidney cells.
In a transformed cell line, derived from baby hamster kidney cells by treatment with ethylnitrosourea, degradation of DNA in isolated nuclei by endogenous nuclease was studied. Compared to the nontransformed cell line, the nuclear DNA of the transformed cells was found to be degraded to a much greater extent. This was reflected by a markedly lower proportion of DNA attached to the nuclear protein matrix in the transformed compared to the nontransformed cells. These observations can be accounted for by assuming that the chromatin of the transformed cell line has a conformation different from that of the nontransformed cells.
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism*; D006224:Cricetinae; D004247:DNA / Q000378:metabolism; D003851:Deoxyribonucleases / Q000494:pharmacology; D007668:Kidney / Q000378:metabolism*
D002843:Chromatin; D004247:DNA; D003851:Deoxyribonucleases