The structural basis for C-banding. A scanning electron microscopy study.
The same C-banded human polymorphic chromosomes were observed in the light microscope (LM) and then in the scanning electron microscope (SEM) to investigate the structural changes produced by the C-banding technique. C-banded regions, which stained positively in LM, were highly condensed with tightly packed chromatin fibres, resembling non-banded chromosomes. In striking contrast, adjacent non-C-banded regions were represented by loosely arranged fibres, resembling G-banded chromosomes. The significance of these observations in relation to current theories on the effects of C-banding on chromosome structure is discussed.
D001399:Azure Stains; D002503:Centromere / Q000648:ultrastructure; D002871:Chromosome Banding; D002875:Chromosomes / Q000648:ultrastructure*; D002900:Chromosomes, Human, 1-3 / Q000648:ultrastructure; D002902:Chromosomes, Human, 16-18 / Q000648:ultrastructure; D002906:Chromosomes, Human, 6-12 and X / Q000648:ultrastructure; D006570:Heterochromatin / Q000648:ultrastructure*; D006358:Hot Temperature; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D011110:Polymorphism, Genetic; D014998:Y Chromosome / Q000648:ultrastructure
D001399:Azure Stains; D006570:Heterochromatin