Rapid microbiochemical method for presumptive identification of gastroenteritis-associated members of the family Enterobacteriaceae.
A method for rapid screening of isolates of pathogenic members of the family Enterobacteriaceae is described. Flow charts are used in conjunction with triple sugar iron agar, o-nitrophenyl-beta-D-galactopyranoside-phenylalanine-motility sulfate screening media, oxidase test, and six rapid biochemical tests, namely, lysine decarboxylase, urease, indole, esculin hydrolysis, malonate, and xylose. This scheme is used to provide an inexpensive but rapid presumptive identification of Salmonella, Shigella, Edwardsiella, Aeromonas, Plesiomonas, Vibrio, and Yersinia isolates from stool cultures.
D003470:Culture Media; D004755:Enterobacteriaceae / Q000145:classification*; D004756:Enterobacteriaceae Infections / Q000175:diagnosis / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005759:Gastroenteritis / Q000382:microbiology*; D006801:Humans; D008832:Microchemistry
D003470:Culture Media