Immunological studies of the uncoupling protein of brown adipose tissue.
The immunological relationship of the uncoupling protein from brown adipose tissue of several mammalian species was examined by using a rabbit antibody preparation against the rat protein. Complete cross-reactivity of the antibody to the protein from hamster, mouse, and rat was found, whereas the protein from rabbit cross-reacted only 25%. Cross-reactivity was also found with the human uncoupling protein, although the human protein was found to be about 1 kdalton smaller than the rat protein. No protein of the size of the uncoupling protein was detected in several tumor cell lines examined.
D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D000918:Antibody Specificity; D002352:Carrier Proteins; D006224:Cricetinae; D006801:Humans; D007473:Ion Channels; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice; D008928:Mitochondria / Q000276:immunology*; D024101:Mitochondrial Proteins; D008970:Molecular Weight; D051381:Rats; D014475:Uncoupling Agents / Q000276:immunology*; D000071256:Uncoupling Protein 1
D002352:Carrier Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D014475:Uncoupling Agents; D000071256:Uncoupling Protein 1