A cost-effective system for performing therapeutic drug assays. I. Optimization of the theophylline assay.
We have developed a cost-effective system for performing drug assays by using the COBAS BIO centrifugal analyzer and EMIT reagents. With the theophylline assay as a model, we were able to optimize both instrument and chemical parameters to allow assay of 2470 samples with a 100-test EMIT kit. This decreased current reagent costs from +2.00 per test for the Syva manual method to 9 cents per test for our proposed method. Within-run and between-run CVs are typically less than 3% and 6%, respectively.
D003365:Costs and Cost Analysis; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000378:metabolism; D006801:Humans; D007202:Indicators and Reagents; D008433:Mathematics; D009243:NAD / Q000378:metabolism; D011933:Reagent Kits, Diagnostic / Q000191:economics*; D013696:Temperature; D013806:Theophylline / Q000097:blood*
D005958:Glucosephosphates; D007202:Indicators and Reagents; D011933:Reagent Kits, Diagnostic; D009243:NAD; D019298:Glucose-6-Phosphate; D013806:Theophylline