Difficulties in the normalization of aminotransferase measurement with enzyme standards.
Commercial control sera contain varying amounts of pyridoxal-5'-phosphate. Normalization of aminotransferase activity measurement with enzyme "standards" is possible only when pyridoxal-5'-phosphate is added to the reaction medium. Pyridoxal-5'-phosphate addition should be compulsory in national recommendations.
D000410:Alanine Transaminase / Q000032:analysis; D001219:Aspartate Aminotransferases / Q000032:analysis; D002384:Catalysis; D004798:Enzymes / Q000592:standards*; D006801:Humans; D011732:Pyridoxal Phosphate / Q000032:analysis; D012015:Reference Standards; D000637:Transaminases / Q000032:analysis* / Q000592:standards
D004798:Enzymes; D011732:Pyridoxal Phosphate; D000637:Transaminases; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase