The hCG-induced increase in hormone uptake and interstitial fluid volume in the rat testis is not mediated by steroids, prostaglandins or protein synthesis.
The possible mediation by steroids, prostaglandins or protein synthesis on the hCG-induced increase in hormone uptake and interstial fluid volume in the rat testis in vivo was studied following a single iv injection of hCG. A high dose of hCG increased its own uptake in the testis and this uptake coincided with an increase in capillary permeability and accumulation of interstial fluid. The possible role of steroids, prostaglandins and protein synthesis in these changes was studied in vivo using aminoglutethimide, indomethacin and cycloheximide, respectively, as inhibitors. However, none of these prevented the hCG-induced changes in uptake and interstial fluid volume, which suggests that they do not mediate this phenomenon.
D000818:Animals; D002199:Capillary Permeability; D006063:Chorionic Gonadotropin / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D003513:Cycloheximide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005110:Extracellular Space / Q000378:metabolism*; D007106:Immune Sera / Q000494:pharmacology; D008297:Male; D011448:Prostaglandin Antagonists / Q000494:pharmacology; D011453:Prostaglandins / Q000096:biosynthesis; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D013256:Steroids / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D013737:Testis / Q000378:metabolism*
D006063:Chorionic Gonadotropin; D007106:Immune Sera; D011448:Prostaglandin Antagonists; D011453:Prostaglandins; D013256:Steroids; D003513:Cycloheximide