Gas chromatographic determination of m- and p-hydroxyphenytoin in the urine of epileptic patients.
An analytical method for determining phenytoin and its metabolites in the urine of epileptic patients is described. The analysis was performed for the m- and p-isomers of hydroxyphenytoin, the oxidative products of phenytoin, using gas chromatography. As an internal standard, 5-(4-methylphenyl)-5-phenylhydantoin was chosen; the extraction solvent from human urine was ether-chloroform (3:7). Phenytoin and its hydroxy isomers were satisfactorily determined by the modified on-column methylation technique on a 2% OV-17 column using temperature programming from 180 degrees C to 240 degrees C at 5 degrees C/min.
D000328:Adult; D000368:Aged; D002220:Carbamazepine / Q000652:urine; D002849:Chromatography, Gas; D004827:Epilepsy / Q000652:urine*; D005260:Female; D006801:Humans; D006868:Hydrolysis; D008297:Male; D010672:Phenytoin / Q000031:analogs & derivatives* / Q000652:urine; D014508:Urea / Q000031:analogs & derivatives / Q000652:urine
C001419:hydroxyphenytoin; D002220:Carbamazepine; C011271:hydantoic acid; D010672:Phenytoin; D014508:Urea