A comparison between centrifugation and filtration as a means to separate bound and unbound ligand during 3[H]-spiroperidol binding.
A centrifugation method for the separation of bound and unbound ligand during 3[H]-spiroperidol binding in rat brain was compared with the more widely used filtration method. The centrifugation method yielded significantly greater total and specific binding, and lower nonspecific binding, than filtration. Assay reproducibility was also found to be superior when the centrifugation method was used as compared to the filtration binding method. Scatchard plots produced by both methods yielded comparable Kd and Bmax values that were consistent with those previously reported.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002090:Butyrophenones / Q000378:metabolism*; D002498:Centrifugation; D003342:Corpus Striatum / Q000378:metabolism; D005374:Filtration; D066298:In Vitro Techniques; D007700:Kinetics; D008024:Ligands; D008297:Male; D009714:Nucleus Accumbens / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013134:Spiperone / Q000378:metabolism*
D002090:Butyrophenones; D008024:Ligands; D013134:Spiperone