Studies on phosphagen synthesis by mitochondrial preparations.
Mitochondrial preparations from muscles of a crab (Cancer pagurus), two fish (Trachurus trachurus and Scyliorhinus canicula) and a bird (Columba livia) are able to synthesise, through ATP, the phosphagen related to that species. This indicates the presence of a bound phosphagen kinase. Addition of creatine kinase and creatine to crab mitochondria results in the synthesis of phosphocreatine. Similarly, the addition of arginine kinase and arginine to mitochondrial preparations from the fish and bird results in the synthesis of phosphoarginine. In the crab, the mitochondrial form of arginine kinase released by sonication had the same kinetic affinity constants and electrophoretic mobility and could not be distinguished immunologically from the cytosolic form. The close similarity of bound and cytosolic forms of arginine kinase in this crustacean suggests that the two forms have not evolved separately as has creatine kinase in the mammal.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D001122:Arginine Kinase / Q000378:metabolism; D003386:Brachyura; D010856:Columbidae; D003402:Creatine Kinase / Q000378:metabolism; D005399:Fishes; D008931:Mitochondria, Muscle / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000096:biosynthesis; D010725:Phosphocreatine / Q000096:biosynthesis*
D009943:Organophosphorus Compounds; D010725:Phosphocreatine; C015441:phospho-L-arginine; D000255:Adenosine Triphosphate; D001120:Arginine; D003402:Creatine Kinase; D001122:Arginine Kinase