Determination of proenkephalin products in brain tissue by high-performance liquid chromatography and a modified bioassay procedure.
A method is described for the separation of proenkephalin products using gradient high-performance liquid chromatography preceded by Sep-Pak chromatography. Samples can be assayed simply by use of a modified mouse vas deferens bioassay which is sufficiently sensitive for most applications. The preliminary Sep-Pak chromatography method excludes alpha-neoendorphin and the dynorphins and thus provides a suitable procedure for separation of prodynorphin and proenkephalin products.
D000818:Animals; D001681:Biological Assay; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D003342:Corpus Striatum / Q000378:metabolism; D004558:Electric Stimulation; D004723:Endorphins / Q000494:pharmacology; D004745:Enkephalins / Q000032:analysis*; D066298:In Vitro Techniques; D007930:Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D008297:Male; D051379:Mice; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D011498:Protein Precursors / Q000032:analysis*
D004723:Endorphins; D004745:Enkephalins; D011498:Protein Precursors; C029992:proenkephalin; D007930:Leucine; C012211:ubenimex