Indirect [3H]methyl exchange as a general method for labeling methionine residues: application to calcitonin.
Native porcine calcitonin from Armour is known to contain two components. It is shown that these can be separated by cation-exchange chromatography in 8 M urea. The technique of [3H]methyl exchange on the methionine residue was used to prepare each of these in a tritiated form. The reduced components formed by demethylation were found to readily reoxidize at neutral pH, to regenerate the disulfide bridge. Evidence is provided to show that the two forms were partially interconverted during these steps. The reoxidized 3H-labeled products were found to be indistinguishable in chemical, immunological, and biological properties from the equivalent components in native porcine calcitonin and had specific activities of approximately 20 Ci/mmol. It is concluded that this labeling method can be conveniently applied to peptides containing one or more disulfide bridges, to give products of high specific activity in acceptable yield, provided appropriate conditions are used to ensure correct reoxidation occurs.
D000818:Animals; D002116:Calcitonin / Q000032:analysis*; D002118:Calcium / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D003640:Dealkylation; D004591:Electrophoresis, Polyacrylamide Gel; D007474:Ion Exchange; D007553:Isotope Labeling / Q000379:methods; D008715:Methionine / Q000032:analysis*; D008745:Methylation; D010084:Oxidation-Reduction; D011863:Radioimmunoassay; D013552:Swine
D002116:Calcitonin; D008715:Methionine; D002118:Calcium