Innervation pattern of muscles of one-legged Xenopus laevis supplied by motoneurons from both sides of the spinal cord.
In Xenopus tadpoles one limb bud was removed before innervation and motoneurons from both sides of the spinal cord were induced to innervate the remaining limb. When examined after metamorphosis the motor innervation of the limb had the following characteristics. In agreement with previous findings a large proportion of contralateral motoneurons survived (51-82% of the ipsilateral numbers) and sent axons to the limb. By acetylcholinesterase staining and intracellular recording from muscle fibers of the response to electrical stimulation of the two limb innervations, the neuromuscular junctions from contralateral motoneurons were indistinguishable from those from the ipsilateral side in their morphology, spacing along the fiber, and physiological properties. Many single muscle fibers shared innervation from both sides of the cord by symmetrically placed spinal nerves. By the same techniques junctions in one-legged frogs were morphologically indistinguishable from those in normal frogs, but the quantal content of transmitter release was increased by up to 63%. Recording twitch and tetanic tensions from individual motor units from the gastrocnemius muscle showed that the one-legged animals had many more and smaller motor units than do normal frogs. We confirm that the hind-limb musculature has the ability, normally unexpressed, to sustain, through the period of normal developmental cell death, up to twice the usual number of motoneurons. In maturity, motoneurons accommodate themselves to the limb muscles by making fewer than the normal number of synapses. The above suggests that developmental motoneuron death is not primarily a mechanism for adjusting the number of motoneurons to the size of the peripheral musculature and is likely to be related to mechanisms for securing specific neuromuscular connections.
D000818:Animals; D000870:Anterior Horn Cells / Q000502:physiology*; D002470:Cell Survival; D004594:Electrophysiology; D006614:Hindlimb / Q000254:growth & development / Q000294:innervation; D007537:Isometric Contraction; D009046:Motor Neurons / Q000502:physiology*; D024510:Muscle Development; D009132:Muscles / Q000294:innervation* / Q000502:physiology; D013127:Spinal Nerves / Q000254:growth & development*; D014982:Xenopus laevis