Determination of 3-mercaptolactic acid by amino acid analyzer after aminoethylation.
A quantitative determination of 3-mercaptolactic acid was performed after its conversion into S-aminoethylmercaptolactic acid by reacting with excess of 2-bromoethylamine. S-aminoethylmercaptolactic acid was quantitated by an amino acid analyzer. Other thiols were shown not to interfere with the determination of 3-mercaptolactic acid. The sensitivity of the method was at the nanomoles level. The application of the method to the determination of 3-mercaptolactic acid in human urine is also reported.
D000603:Amino Acids, Sulfur / Q000032:analysis* / Q000138:chemical synthesis; D001322:Autoanalysis / Q000295:instrumentation; D005021:Ethylamines; D005260:Female; D006801:Humans; D008297:Male; D013438:Sulfhydryl Compounds / Q000652:urine*
D000603:Amino Acids, Sulfur; D005021:Ethylamines; D013438:Sulfhydryl Compounds; C004504:2-bromoethylamine; C008361:beta-mercaptolactate; C037862:S-aminoethylmercaptolactic acid