In vivo electrochemical monitoring of apomorphine penetration into the rat brain.
Differential normal pulse voltammetry was used together with electrochemically treated carbon-fibre electrodes to monitor apomorphine (APO) penetration into the cortex of anaesthetized rats after a peripheral administration of this drug. In vivo cortical APO extracellular concentration and post-mortem APO levels both measured by means of this technique were estimated to be, respectively, 105 +/- 8 nM and 14.6 +/- 1.1 nmol/g of tissue. The maximal extracellular concentration was reached 16 min after a s.c. injection and reached a plateau beyond a dose of 4 mg/kg (s.c.). This suggests that APO is mainly trapped within brain cells.
D000818:Animals; D001058:Apomorphine / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D004563:Electrochemistry / Q000379:methods; D005110:Extracellular Space / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008991:Monitoring, Physiologic / Q000379:methods*; D009994:Osmolar Concentration; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D001058:Apomorphine