Topical glucocorticoids and suppression of contact sensitivity. A mouse bioassay of anti-inflammatory effects.
A mouse model for assessment of the anti-inflammatory effect of topical glucocorticoids is described. Mice, sensitized to picryl chloride, had both ears painted with the sensitizer followed 2 hours later by the application of the topical steroid to one ear and the corresponding vehicle to the other. The contact sensitivity reaction, measured by swelling of the ear, was recorded 24 hours later. Dilutions of the steroid formulations inhibited the ear swelling in a manner related to dose-response. Suppression of the contact sensitivity reaction of the vehicle-treated ear as well was regarded as a systemic effect of the glucocorticoid. There seems to be a good correlation between the efficacy of the topical steroids assessed in this mouse model and the vasoconstrictor test on intact human skin.
D000287:Administration, Topical; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D001681:Biological Assay; D002990:Clobetasol / Q000031:analogs & derivatives / Q000627:therapeutic use; D003877:Dermatitis, Contact / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D004195:Disease Models, Animal; D004306:Dose-Response Relationship, Immunologic; D004431:Ear, External / Q000473:pathology; D005938:Glucocorticoids; D006854:Hydrocortisone; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D010853:Picryl Chloride
D000893:Anti-Inflammatory Agents; D005938:Glucocorticoids; D002990:Clobetasol; D006854:Hydrocortisone; D010853:Picryl Chloride