Differential alterations by chronic treatment with morphine of pro-opiomelanocortin mRNA levels in anterior as compared to intermediate pituitary lobes of rats.
Chronic treatment of rats with morphine by implantation of pellets over a period of 10 days resulted in a differential alteration of pro-opiomelanocortin (POMC) mRNA levels in individual pituitary lobes: Hybridisation studies using a 32P-labelled mouse POMC cDNA fragment of 150 bases as a probe revealed that chronic morphine treatment causes about 50% enhancement of POMC mRNA levels in the anterior lobe and about 40% decrease in the intermediate lobe of the pituitary. Significant changes were observed 3 days following commencement of administration and persisted over the rest of the morphine treatment.
D000818:Animals; D008297:Male; D009020:Morphine / Q000494:pharmacology*; D009693:Nucleic Acid Hybridization; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D011333:Pro-Opiomelanocortin / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
D012333:RNA, Messenger; D011333:Pro-Opiomelanocortin; D009020:Morphine