Inhibition of a soman- and diisopropyl phosphorofluoridate (DFP)-detoxifying enzyme by Mipafox.
Mipafox, N,N'-diisopropylphosphordiamidofluoridate, has been found to be a reversible competitive inhibitor of a diisopropyl phosphorofluoridate hydrolyzing enzyme (DFPase) isolated from hog kidney and Escherichia coli. Heretofore, this DFPase was characterized by its more rapid hydrolysis of Soman (1,2,2-trimethylpropyl methylphosphonofluoridate), its stimulation by Mn2+, and its wide distribution. In sharp contrast, Mipafox did not inhibit the DFPase found only in cephalopod nerve, hepatopancreas, and saliva, and further characterized by its more rapid hydrolysis of DFP than of Soman, and its indifference to Mn2+. Neither of these two DFPases hydrolyzed Mipafox.
D000818:Animals; D004950:Esterases; D006801:Humans; D006867:Hydrolases / Q000037:antagonists & inhibitors*; D006868:Hydrolysis; D007531:Isoflurophate / Q000031:analogs & derivatives* / Q000494:pharmacology; D009943:Organophosphorus Compounds / Q000378:metabolism*; D044345:Phosphoric Triester Hydrolases; D012999:Soman / Q000378:metabolism*
D009943:Organophosphorus Compounds; D007531:Isoflurophate; C005238:mipafox; D012999:Soman; D006867:Hydrolases; D004950:Esterases; D044345:Phosphoric Triester Hydrolases; C020061:diisopropyl-fluorophosphatase