Uncoupler- and hypoxia-induced damage in the working rat heart and its treatment. I. Observations with uncouplers of oxidative phosphorylation.
In the isolated working rat heart, the damaging effect of 0.05-0.06 microM Carbonylcyanide-p-trifluoro-methoxyphenylhydrazone (FCCP) was reversible within 20 sec by perfusion with fresh buffer. Cysteine 3 mM restored the aortic flow to the initial value within an additional 15-20 sec. Thereafter, the FCCP effect became irreversible due to a progressive structural membrane change. The structural change by FCCP is probably brought about by 'internalization' of polar groups (R-SH; R-NH3+) of the mitochondrial (and other) membrane.
D000818:Animals; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology*; D003326:Coronary Circulation / Q000187:drug effects; D003327:Coronary Disease / Q000139:chemically induced* / Q000503:physiopathology; D003545:Cysteine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism / Q000187:drug effects*; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects; D009206:Myocardium / Q000378:metabolism*; D009570:Nitriles / Q000494:pharmacology*; D010710:Phosphates / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D011919:Rats, Inbred Strains
D009570:Nitriles; D010710:Phosphates; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D003545:Cysteine