Immunohistochemical and biochemical studies on DNA ligase from a rat liver parenchymal cell line.
We have recently obtained a monospecific antibody against calf thymus DNA ligase composed of a single Mr = 130,000 polypeptide. Immunohistochemical studies using the antibody and immunoperoxidase detection methods indicated that DNA ligase in a rat liver parenchymal cell line (BB) is localized essentially in nucleus. The specific activity of DNA ligase from growing BB cells was more than 10-fold higher than that from rat hepatocytes. The molecular forms of DNA ligase in these cell-free extracts were also analyzed.
D000818:Animals; D000918:Antibody Specificity; D002460:Cell Line; D002467:Cell Nucleus / Q000201:enzymology; D011088:DNA Ligases / Q000276:immunology* / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D011118:Polynucleotide Ligases / Q000276:immunology*; D051381:Rats; D013950:Thymus Gland / Q000201:enzymology
D011088:DNA Ligases; D011118:Polynucleotide Ligases