Isolation of a teratocarcinoma stem cell lectin implicated in intercellular adhesion.
We have previously identified a cell surface teratocarcinoma stem cell lectin with a fucan/mannan specificity. We now report the purification of the hemagglutinin (lectin) from stem cell conditioned medium by exclusion on a Sepharose 2B column, followed by elution with 0.5M NaCl from DEAE-cellulose, providing an overall purification of about 90-fold. When this material was analyzed, by SDS-polyacrylamide gel electrophoresis, a major band of Mr 56000 was consistently observed. Hemagglutination activity was renatured from the gels and localized exclusively to a region of the gel that, as detected by fluorography, contains only the 56-kDa component. This suggested that this polypeptide comprises the lectin.
D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D054278:Embryonal Carcinoma Stem Cells; D005470:Fluorometry; D006386:Hemagglutination Tests; D037102:Lectins / Q000302:isolation & purification*; D014411:Neoplastic Stem Cells / Q000032:analysis*; D017830:Octoxynol; D011092:Polyethylene Glycols; D013234:Stem Cells / Q000032:analysis*; D013724:Teratoma / Q000032:analysis*
D037102:Lectins; D011092:Polyethylene Glycols; D017830:Octoxynol