Improved methodology for the production of monoclonal antibodies against parasites.
A modification of the standard fusion methodology is described which results in greatly increased yields of monoclonal antibodies against certain organ-specific parasites. Several fusions were carried out using mice infected with Schistosoma mansoni or Nematospiroides dubius, using B lymphocytes harvested from either the spleen or the mesenteric lymph nodes. Results indicated a greatly improved yield of positive clones using the lymph nodes as a source of B cells for fusion. A 7-fold increase in the number of positive clones was seen with N. dubius injections, while S. mansoni fusions showed a 2-fold increase.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology*; D005260:Female; D006376:Helminths / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D012550:Schistosoma mansoni / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth