A simplified procedure for the in vitro assay of the initial linear rate of the reaction of lecithin-cholesterol acyltransferase in human serum.
A simple sensitive method for the determination of the initial rate of the reaction of lecithin-cholesterol acyltransferase by equilibrating [3H]cholesterol with unesterified cholesterol of human serum is described. The resulting serum is incubated for various time periods at 37 degrees C and the increase of the label in the cholesterol ester fraction is measured. The labeling is effected by a filter paper method in which a paper strip containing the labeled cholesterol is placed in serum at 4 degrees C, thereby preventing the formation of labeled cholesterol esters by the action of the enzyme. The rate of the reaction was linear up to 30 min.
D000096:Acetone; D002784:Cholesterol / Q000378:metabolism; D002788:Cholesterol Esters / Q000097:blood; D005374:Filtration; D006801:Humans; D007553:Isotope Labeling; D007700:Kinetics; D008074:Lipoproteins / Q000097:blood; D008722:Methods; D002785:Sterol O-Acyltransferase / Q000097:blood*; D013997:Time Factors; D014316:Tritium
D002788:Cholesterol Esters; D008074:Lipoproteins; D014316:Tritium; D000096:Acetone; D002784:Cholesterol; D002785:Sterol O-Acyltransferase