D-galactosamine-induced liver injury: a rat model to study the heterogeneity of the oligosaccharide chains of alpha 1-acid glycoprotein.
The effect of D-galactosamine on the structure of the glycan moiety of alpha 1-acid glycoprotein was studied throughout a nine days experiment. It was shown that: D-galactosamine led to an alteration of the Concanavalin A crossed immunoelectrophoresis pattern and to a decreased sialic acid content of alpha 1-acid glycoprotein. The undersialylation of alpha 1-acid glycoprotein was not linked to a change in the relative ratio of various Concanavalin A forms. At the end of the experiment (9 days after galactosamine injection), the Concanavalin A non-reactive forms of alpha 1-acid glycoprotein remained elevated whereas alanine transaminase activity, total protein and alpha-acid glycoprotein had returned to a control level. D-galactosamine-treated rats seem to be a suitable model for the study of the very fast cyclic modulations of the synthesis of the glycan moiety of glycoproteins.
D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood*; D003208:Concanavalin A / Q000032:analysis; D004195:Disease Models, Animal; D005688:Galactosamine / Q000633:toxicity*; D007123:Immunoelectrophoresis, Two-Dimensional; D008297:Male; D009844:Oligosaccharides / Q000032:analysis*; D009961:Orosomucoid / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D012794:Sialic Acids / Q000032:analysis; D013997:Time Factors
D009844:Oligosaccharides; D009961:Orosomucoid; D012794:Sialic Acids; D003208:Concanavalin A; D005688:Galactosamine