[Physicochemical properties of the RNA and proteins of an influenza virus H1N3 isolated from an ill child and antigenically analogous to A/whale/TO/19/76].
A comparative analysis of RNA and proteins of influenza A/Baku/799/82, A/whale/TO/19/76, and A/PR8/34 viruses was carried out. The viruses were shown to be similar in their polypeptide composition and oligopeptide maps of the heavy (HA1) and light (HA2) chains of hemagglutinin; in their migration properties of RNA fragments in polyacrylamide gel the A/Baku/799/82 and A/whale/TO/19/76 viruses were similar but not identical. Marked differences in the electrophoretic mobility in gel of RNA fragments coding for P proteins, HA, NP, and NA polypeptides were demonstrated. All these fragments of A/whale/TO/19/76 virus had higher electrophoretic mobility in gel. RNA fragments coding for M and NS proteins had a similar electrophoretic mobility. The A/Baku/799/82 and A/whale/TO/19/76 viruses differed considerably in migration properties of the RNA fragment coding for neuraminidase from the epidemic A/PR8/34 virus. In the latter, this fragment had a higher electrophoretic mobility in gel. Experiments of RNA-RNA hybridization demonstrated a high degree of homology of the primary structure of all RNA fragments of A/Baku/799/82 and A/whale/TO/19/76 viruses.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002591:Cetacea / Q000382:microbiology*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002648:Child; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D009980:Influenza A virus / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D007251:Influenza, Human / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D009842:Oligopeptides / Q000032:analysis; D010455:Peptides / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis*; D014764:Viral Proteins / Q000032:analysis*; D014907:Whales / Q000382:microbiology*
D000956:Antigens, Viral; D009842:Oligopeptides; D010455:Peptides; D012367:RNA, Viral; D014764:Viral Proteins
Izucheniefiziko-khimicheskikh svoĭstv RNK i belok virusa grippa H1N3, izolirovannogo ot bol'nogo rebenka i antigenno analogichnogo virusu A/kit/TO/19/76.