Radioimmunoassay for serum and urinary Tamm-Horsfall glycoprotein in the rat.
Rat Tamm-Horsfall glycoprotein (THG) was isolated from urine and disaggregated by gel filtration. Purity was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and autoradiography. Antibodies were raised and used in the development of a specific radioimmunoassay for rat THG. Parallelism could be demonstrated between the standard preparation and urine (diluted to 1:320 with distilled water) and serum (at all dilutions) samples. Recovery of standard added to urine or serum was on average 93% and inter- and intra-assay variation were both below 7%. There was a positive correlation between the mean urinary excretory rate of THG (microgram/24h) and body weight in female rats (p less than 0.001) but not in male rats. Serum THG levels were similar in both sexes and showed a positive correlation with body weight (p less than 0.001; range 43-240 ng/ml). It is concluded that the rat urinary THG excretion rate is similar to that in man and that rat serum also contains a THG-like protein.
D000818:Animals; D001835:Body Weight; D005260:Female; D007106:Immune Sera; D008297:Male; D009088:Mucoproteins / Q000652:urine*; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution; D058949:Uromodulin
D007106:Immune Sera; D009088:Mucoproteins; C548614:Umod protein, rat; D058949:Uromodulin