The potency of heparin-like activity of glycosaminoglycans released by human endothelial cells in culture.
Glycosaminoglycans isolated from culture medium conditioned by human endothelial cells showed heparin-like anti-thrombin III cofactor activity measured by Xa inhibition. Their activity was relatively weak, 0.1% of the potency of heparin, but was approximately 5-fold more potent than that of glycosaminoglycans derived from vascular smooth muscle cells.
D001777:Blood Coagulation / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D004727:Endothelium / Q000502:physiology*; D005170:Factor X / Q000037:antagonists & inhibitors*; D006025:Glycosaminoglycans / Q000494:pharmacology*; D006801:Humans; D013329:Structure-Activity Relationship
D003470:Culture Media; D006025:Glycosaminoglycans; D005170:Factor X