Orotic acid, a promoter of liver carcinogenesis induces DNA damage in rat liver.
Orotic acid, a precursor of pyrimidine nucleotide biosynthesis and a promoter for liver carcinogenesis, when fed at 1% level in a diet for 5 weeks resulted in liver DNA damage. The damage can be monitored as alkali-labile lesions using alkaline sucrose gradients as well as alkaline elution technique. Furthermore, the induced DNA damage persists for up to three weeks after withdrawal of the orotic acid diet. The fact that several skin-tumour promoters also induce DNA damage raises the question whether DNA damage is a component in tumour promotion.
D000818:Animals; D002273:Carcinogens; D004247:DNA; D008099:Liver / Q000187:drug effects*; D008113:Liver Neoplasms / Q000139:chemically induced*; D008297:Male; D009963:Orotic Acid / Q000633:toxicity*; D051381:Rats; D011916:Rats, Inbred F344
D002273:Carcinogens; D009963:Orotic Acid; D004247:DNA