5,6-Dichlorobenzimidazole-1-beta-D-riboside, specific inhibitor of cytokinin activity in tobacco cell suspension cultures.
Tobacco cell suspension cultures were supplemented with 4 to 10 microM 5,6-dichloro-1-beta-D-ribofuranosyl-benzimidazole (DBR). The cell suspensions were cultured in the presence or without a cytokinin, kinetin (N6-furfuryladenine). The responses of the cultures to DRB and (or) kinetin were measured by the evolution of cell mass or cell counts in the cultures, as a function of time or kinetin concentration. Chlorophyll biosynthesis was also used as a test-response to cytokinin and (or) DRB activities. It was concluded that DRB behaves as a specific inhibitor of the physiological responses to cytokinins. The evolution of the inhibition ratio versus kinetin concentration shows that DRB is not a competitor of the cytokinins.
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002734:Chlorophyll / Q000096:biosynthesis; D002736:Chloroplasts / Q000378:metabolism; D004004:Dichlororibofuranosylbenzimidazole / Q000494:pharmacology*; D007700:Kinetics; D007701:Kinetin; D059828:Plant Cells; D010944:Plants / Q000378:metabolism*; D010947:Plants, Toxic; D012263:Ribonucleosides / Q000494:pharmacology*; D013997:Time Factors; D014026:Nicotiana
D012263:Ribonucleosides; D002734:Chlorophyll; D004004:Dichlororibofuranosylbenzimidazole; D000225:Adenine; D007701:Kinetin