Lectin-specific targeting of beta-glucocerebrosidase to different liver cells via glycosylated liposomes.
Galactosylated and mannosylated liposomes were more efficient in transporting liposome-entrapped beta-glucocerebrosidase to liver compared to nonglycosylated liposomes. The enzyme entrapped to glycoside-bearing liposomes was found to be cleared at a much faster rate than that entrapped in liposomes having no sugar on their surface. Asialoorosomucoid and hydrolyzed mannan were found to inhibit both the clearance and the uptake of galactosylated and mannosylated liposomes, respectively, supporting involvement of lectin-sugar interaction. Further studies on the uptake of glucocerebrosidase by isolated liver cells revealed that the enzyme entrapped in mannosylated liposomes has much higher affinity for nonparenchymal cells whereas the assimilation of the entrapped enzyme into hepatocytes is clearly favored for liposomes having galactose on their surface.
D000818:Animals; D005260:Female; D005959:Glucosidases / Q000008:administration & dosage*; D005962:Glucosylceramidase / Q000008:administration & dosage*; D006801:Humans; D037102:Lectins; D008081:Liposomes / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains
D037102:Lectins; D008081:Liposomes; D005959:Glucosidases; D005962:Glucosylceramidase