Sensitized photo-oxidation of bovine alpha-crystallin: change in microenvironments of thiol groups.
Methylene blue- or riboflavin-sensitized photo-oxidation of alpha-crystallin caused a major change in the environment of thiol groups of the protein which could not be significantly prevented by inhibitors specific for active species of oxygen, 1O2, .OH, O.2, and H2O2. In contrast, the sensitizer N-formylkynurenine had no such effect. Sensitizer-induced change in the microenvironments of thiol groups was attributed to a conformational alteration, probably an unfolding of the protein.
D000818:Animals; D002417:Cattle; D003459:Crystallins / Q000528:radiation effects*; D007737:Kynurenine / Q000031:analogs & derivatives; D008751:Methylene Blue; D010084:Oxidation-Reduction; D010777:Photochemistry; D011487:Protein Conformation / Q000528:radiation effects; D012256:Riboflavin; D013438:Sulfhydryl Compounds / Q000528:radiation effects
D003459:Crystallins; D013438:Sulfhydryl Compounds; C007772:N'-formylkynurenine; D007737:Kynurenine; D008751:Methylene Blue; D012256:Riboflavin