Leukocyte concentration and the yields of metaphase cells in cultured peripheral blood of leukemic patients with hyperleukocytosis.
The optimal concentration of leukocytes for maximizing the yields of metaphase cells was examined using PHA-stimulated and unstimulated peripheral blood cells of various leukemic patients with hyperleukocytosis, including 5 acute nonlymphocytic leukemias, 4 Ph-positive chronic myelocytic leukemias, one acute lymphoblastic leukemia, and one malignant lymphoma at a leukemic phase. The yields of metaphases reached maximum at leukocyte concentrations of either 1 X 10(6) or 2 X 10(6)/ml in both stimulated and unstimulated blood cultures of each patient, except for one patient with malignant lymphoma who exhibited no metaphases at any cell concentration level in the unstimulated culture. Metaphase yields in the stimulated and unstimulated cultures correlated with neither the percentages of blast cells and lymphocytes in the peripheral blood of the leukemia patients nor with the type of leukemia studied.
D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007938:Leukemia / Q000150:complications / Q000473:pathology*; D007958:Leukocyte Count; D007964:Leukocytosis / Q000150:complications / Q000473:pathology*; D008297:Male; D008677:Metaphase; D008875:Middle Aged