The effect of albumin on the uptake of bromosulfophthalein by isolated rat hepatocytes.
The initial rate of uptake of bromosulfophthalein (BSP) by isolated rat hepatocytes analyzed as a function of the unbound concentration of BSP was greater in the presence of albumin than in its absence, though the presence of albumin in the medium apparently decreased the uptake of BSP by hepatocytes analyzed as a function of the total concentration of BSP. This suggests that the uptake of BSP by isolated rat hepatocytes in the presence of albumin is not only dependent on the unbound concentration of BSP, but also is driven by the bound fraction of BSP.
D000818:Animals; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013448:Sulfobromophthalein / Q000378:metabolism*
D012709:Serum Albumin; D013448:Sulfobromophthalein; D012710:Serum Albumin, Bovine