[Macrolide antibiotics: characteristics of their interaction with organoids, intracellular distribution and the effect of sulfalene].
Interaction of erythromycin and oleandomycin with isolated subcellular fractions of rat liver homogenates and intracellular distribution of the antibiotics were studied. It was found that the macrolides were bound by the cell organoids. The binding was partially reversible. The intracellular distribution was characterized by accumulation of erythromycin and oleandomycin in the mitochondrial fraction while erythromycin preserved higher activity in cytosol as compared to oleandomycin. Sulfalen induced redistribution of the macrolides. It decreased absorption of the macrolides by the organoids and increased the concentration of free erythromycin and especially oleandomycin in cytosol.
D000818:Animals; D001665:Binding Sites; D002463:Cell Membrane Permeability; D002467:Cell Nucleus / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004917:Erythromycin / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism; D009827:Oleandomycin / Q000378:metabolism*; D009940:Organoids / Q000378:metabolism; D051381:Rats; D013415:Sulfalene / Q000494:pharmacology
D004917:Erythromycin; D009827:Oleandomycin; D013415:Sulfalene
Antibiotiki-makrolidy: osobennosti vzaimodeĭstviia s organoidami, vnutrikletochnoe raspredelenie i vliianie na nego sul'falena.