Development of chemosensitivity of an identified insect interneurone.
Characteristic features of cockroach embryos (Periplaneta americana) are determined for different stages in development. Morphological changes in giant interneurone 2 (GI 2) in the terminal abdominal ganglion during embryogenesis are described based on cobalt injections. A sequential proliferation of branches preceded by filopodial extension is observed between 45 and 80% embryogenesis, by which time the characteristic arborization of the first instar cell is established. The cholinergic agonist carbamylcholine was ionophoretically applied to the cell body and dendritic region of GI 2 at various stages in development, and the responses were recorded intracellularly. Chemosensitivity of GI 2 appears at 40 to 45% embryogenesis, to a similar degree in both cell body and dendrites. The sensitivity of these areas subsequently diverges, and after the 60 to 65% stage, the dendrites are approximately 1000 times more sensitive than the cell body. At 80 to 85% embryogenesis, there is a temporary peak in dendritic sensitivity, and cell body sensitivity increases during postembryonic development. The changes in sensitivity take place before synaptogenesis, and the decline in dendritic sensitivity after the 80% stage may be related to synaptic maturation.
D000109:Acetylcholine / Q000502:physiology; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D003058:Cockroaches / Q000196:embryology; D003712:Dendrites / Q000187:drug effects / Q000502:physiology; D004594:Electrophysiology; D007395:Interneurons / Q000187:drug effects / Q000502:physiology*; D013569:Synapses / Q000502:physiology
D002217:Carbachol; D000109:Acetylcholine