Influence of silymarin and some flavonoids on lipid peroxidation in human platelets.
Sixteen natural phenolics and semisynthetic derivatives thereof, including silymarin, flavonoids, catechines and phenolic acids, together with 6 standard drug substances with anti-inflammatory, antioxidant and peroxide radical scavenging properties have been tested in an in vitro model using human platelets for their inhibitory action against N-ethyl maleimide-induced lipid peroxidation. The malondialdehyde formed during the reaction was estimated by means of the thiobarbituric acid method. In all compounds tested, the inhibitory action was clearly dose-dependent; IC50 values were calculated from the dose-activity curves. The IC50 found ranged from between 1.47 nM and approximately 900 mM.
D001792:Blood Platelets / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000097:blood*; D008315:Malondialdehyde / Q000097:blood; D010544:Peroxidases / Q000037:antagonists & inhibitors; D012838:Silymarin / Q000494:pharmacology*
D005419:Flavonoids; D008054:Lipid Peroxides; D012838:Silymarin; D008315:Malondialdehyde; D010544:Peroxidases