Detection of anti-contractile antibodies after cardiac surgery using ELISA assay.
Sera from 196 patients were collected before and after cardiac surgery to measure antibodies against heart tissue and against actin and myosin. In the post-operative period antibodies were found in 87 patients (44%) producing a cross-striated fluorescence pattern in heart tissue. Antibodies against the major contractile proteins were found in 91 patients (46%), anti-actin antibodies in 49 patients (25%) and anti-myosin antibodies in 65 patients (33%). We found a significant correlation (P less than 0.0001) between the antibodies producing a cross-striated fluorescence pattern and antibodies recognizing contractile proteins. These results suggest that contractile proteins evoke an immune response after cardiac surgery and that this response may compromise cardiac function.
D000199:Actins / Q000276:immunology; D000328:Adult; D000918:Antibody Specificity; D001323:Autoantibodies / Q000032:analysis*; D006348:Cardiac Surgical Procedures; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D009206:Myocardium / Q000276:immunology*; D009218:Myosins / Q000276:immunology; D011184:Postoperative Period; D013997:Time Factors
D000199:Actins; D001323:Autoantibodies; D009218:Myosins