Cloning of hybridomas by a single-cell transfer technique.
A method for cloning hybridomas is described which involves transfer of single cells with a capillary tubing connected to a suction apparatus. This method enables the efficient recovery of antibody-producing clones of differing abundances from the parent hybridoma cultures. The 4 cell lines submitted to this cloning procedure produced a 100% yield of positive wells upon recloning by the same method.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000917:Antibody Formation; D002478:Cells, Cultured; D002999:Clone Cells; D006825:Hybridomas / Q000166:cytology*; D051379:Mice
D000911:Antibodies, Monoclonal