Pleiotropic mutants of NIH 3T3 cells with altered regulation in the expression of both type I collagen and fibronectin.
Transformation of NIH 3T3 fibroblasts by v-mos causes a decrease in the levels of type I collagen RNA. In NIH 3T3 cells that have been made resistant to G418 by transfection with a plasmid in which the mouse alpha 2(I) collagen promoter is linked to the neo gene, subsequent v-mos transformation causes a loss of G418 resistance. After mutagenesis of these v-mos-transformed cells, G418-resistant colonies were selected. Two of these G418-resistant mutants showed an increased expression of the neo gene and of the endogenous type I collagen and fibronectin genes, without changes in their levels of v-mos RNA or in their ability to induce tumors. The mutations might alter cellular trans-acting factors that either directly or indirectly control the expression of the type I collagen and fibronectin genes in transformed cells.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003094:Collagen / Q000096:biosynthesis / Q000235:genetics*; D004351:Drug Resistance; D005353:Fibronectins / Q000096:biosynthesis / Q000235:genetics*; D005786:Gene Expression Regulation; D005839:Gentamicins / Q000494:pharmacology; D051379:Mice; D009154:Mutation; D009857:Oncogenes; D011401:Promoter Regions, Genetic; D014162:Transfection
D005353:Fibronectins; D005839:Gentamicins; D003094:Collagen; C010680:antibiotic G 418