Diminution of circulating polymorphonuclear leucocytes by administration of a monoclonal antibody reacting with granulocytes and monocytes.
The monoclonal antibody 3A35 which binds mouse polymorphonuclear leucocytes (PMN) and monocytes was injected intravenously (i.v.) into normal mice. A great diminution of circulating PMN was observed. The percentage of PMN passed through a minimum (2.5%) 20 min after antibody injection and returned to normal value (18.3%) within 24 h. After repeated daily injections, the ability of the antibody to induce granulopenia attenuated. Moreover, mice bearing the 3A35-producing hybridoma as an ascitic tumor had a normal percentage of blood PMN and a normal granulopoiesis as judged from bone marrow cytological examination. Thus, the monoclonal antibody produced a transitory diminution of PMN but could not induce a lasting granulopenia.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001201:Ascites / Q000097:blood; D005260:Female; D006098:Granulocytes / Q000276:immunology*; D006410:Hematopoiesis; D006825:Hybridomas / Q000276:immunology; D007958:Leukocyte Count; D051379:Mice; D009000:Monocytes / Q000276:immunology*; D009504:Neutrophils / Q000502:physiology*
D000911:Antibodies, Monoclonal