Polymerization of N-(1-pyrenyl) iodoacetamide-labelled actin: the fluorescence signal is not directly proportional to the incorporation of the monomer into the polymer.
In the course of the polymerization of N-(1-pyrenyl) iodoacetamide-labelled actin the increase of the fluorescence is not directly proportional to the amount of the polymer mass formed. This precludes the use of the fluorescence signal as a tool for the quantitative description of the polymerization process.
D000199:Actins / Q000378:metabolism*; D000818:Animals; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D007460:Iodoacetamide / Q000031:analogs & derivatives; D007700:Kinetics; D011108:Polymers / Q000378:metabolism*; D011817:Rabbits; D013050:Spectrometry, Fluorescence
D000199:Actins; D005456:Fluorescent Dyes; D011108:Polymers; C029358:N-(1-pyrenyl)iodoacetamide; D007460:Iodoacetamide