Granulocytic and stromal progenitors in the bone marrow of patients with primary myelofibrosis.
Quantitative and qualitative changes in granulocyte-macrophage (CFU-GM) and fibroblast colony-forming cells (CFU-F) were studied in 7 patients with primary myelofibrosis (MF). Marrow cells were collected from bone biopsy specimens after treatment with collagenase. The number of CFU-GM correlated with the amount of haemopoietic tissue noted in the bone marrow histology and ranged between 0-400/mg of bone. CFU-F were increased in 2 patients with moderate fibrosis. Circulating CFU-GM were increased in all patients studied (169-3749/ml of blood). There was no significant correlation between the number of CFU-GM in the bone marrow and that in the blood. Cytochemical studies showed a high incidence in eosinophil progenitors in the bone marrow and especially in the blood of patients with MF. These data suggest a functional abnormality of myeloid progenitors in this disease.
D000368:Aged; D001854:Bone Marrow Cells; D001856:Bone Marrow Examination; D003470:Culture Media; D004804:Eosinophils / Q000166:cytology; D005260:Female; D005347:Fibroblasts; D006412:Hematopoietic Stem Cells; D006801:Humans; D008297:Male; D009504:Neutrophils / Q000166:cytology; D055728:Primary Myelofibrosis / Q000097:blood* / Q000150:complications; D013163:Splenomegaly / Q000209:etiology
D003470:Culture Media