The Kidney Invasion Test: an assay allowing macroscopic quantification of malignant invasion in vivo. Comparison between the microtubule inhibitor tubulozole and the podophyllotoxin congener etoposide (VP-16-213).
When MO4 tumor fragments were implanted under the renal capsule of syngeneic or allogeneic mice, invasion of the renal parenchyma was observed. Inhibition of invasion by the microtubule inhibitor tubulozole could be macroscopically quantified and compared with the invasion permissive drug etoposide (VP-16-213). These results have been histologically confirmed, and the use of this assay in vivo for the rapid macroscopical screening of compounds with potential anti-invasive properties is discussed.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004148:Dioxolanes / Q000627:therapeutic use* / Q000633:toxicity; D004149:Dioxoles / Q000627:therapeutic use*; D004353:Drug Evaluation, Preclinical; D005047:Etoposide / Q000627:therapeutic use* / Q000633:toxicity; D007680:Kidney Neoplasms / Q000188:drug therapy / Q000473:pathology*; D008297:Male; D051379:Mice; D009361:Neoplasm Invasiveness / Q000473:pathology*; D009368:Neoplasm Transplantation; D011034:Podophyllotoxin / Q000031:analogs & derivatives*
D004148:Dioxolanes; D004149:Dioxoles; C040422:tubulazole; D005047:Etoposide; D011034:Podophyllotoxin