Heterogeneous progeny viruses are produced by a budding enveloped phage.
Infection of Acholeplasma laidlawii cells by the temperate enveloped mycoplasma virus L2 results in production of three morphological forms of progeny L2 virus: L2-I, L2-II, and L2-III. These morphological forms can be separated by velocity sedimentation and agarose gel electrophoresis. The latter technique was used to size the quasi-spherical particles: L2-I is 74 nm, L2-II is 88 nm, and L2-III is 132 nm in diameter. The protein composition of the three L2 forms is the same, although there are differences in protein stoichiometric ratios. L2-I, L2-II, and L2-III have the same 11.8 kilobase pair superhelical DNA genome. However, UV inactivation studies and restriction frequency measurements indicate that L2-I and L2-III each contain 1 genome copy, while L2-II contains 2-3 genome copies.
D000128:Acholeplasma laidlawii; D001435:Bacteriophages / Q000032:analysis / Q000254:growth & development* / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008854:Microscopy, Electron; D014771:Virion / Q000032:analysis
D004279:DNA, Viral