Interaction of reduced glutathione with bovine brain tubulin.
Incubation of phosphocellulose-purified tubulin with GSH at 30 degrees C results in an inhibition of colchicine binding activity. GSSG has a protective effect against the GSH-induced loss of colchicine-binding. Incubation of tubulin with GSH at 30 degrees C results in the formation of abnormal tubulin polymers which are insensitive to cold. Such aggregation is insensitive to antimicrotubular drugs. Aggregation is inhibited by GSSG but not by DTT or mercaptoethanol. GSH-induced aggregation is very sensitive to the ionic strength of the assembly medium; both the aggregation and colchicine binding inhibition induced by GSH are inhibited at higher ionic strength. These results indicate a very complex interaction of GSH with tubulin.
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D003078:Colchicine / Q000378:metabolism; D005978:Glutathione / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D019803:Glutathione Disulfide; D008453:Maytansine / Q000494:pharmacology; D008854:Microscopy, Electron; D009994:Osmolar Concentration; D011034:Podophyllotoxin / Q000494:pharmacology; D011108:Polymers / Q000378:metabolism; D013696:Temperature; D014404:Tubulin / Q000378:metabolism*
D011108:Polymers; D014404:Tubulin; D008453:Maytansine; D005978:Glutathione; D011034:Podophyllotoxin; D003078:Colchicine; D019803:Glutathione Disulfide