Gas-chromatographic determination of cholesterol in serum: candidate reference method.
We present a candidate Reference Method for determination of total cholesterol in serum. The method is based on high-resolution capillary gas chromatography, and we took special precautions with respect to weighing, calibration, and chromatographic peak integration. Analytical recovery of added cholesterol was essentially complete (99.99%, SD 0.48%) and reproducibility was excellent (total CV 0.35-0.50%). When cholesterol was determined in a reference serum certified by means of an established candidate Definitive Method, no significant bias could be detected.
D055598:Chemical Phenomena; D002621:Chemistry; D002784:Cholesterol / Q000097:blood*; D002849:Chromatography, Gas; D006801:Humans; D006868:Hydrolysis; D012015:Reference Standards
D002784:Cholesterol