Dual functions of DNA synthesis inhibitory peptide in fetal calf serum.
A novel factor in fetal calf serum was found which showed the dual inhibitory effects on DNA synthesis of murine lymphocytes and some species of bacteria. The factor was purified by gel filtration, ion exchange and thin-layer chromatography and characterized to be a 1 kDa basic peptide. In addition, this factor is not nonspecific to inhibitors, thymidine or polyamines. The modes of action and the spectrum of target cells were analyzed and its biological significance is discussed.
D000818:Animals; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000032:analysis; D004247:DNA / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D006131:Growth Inhibitors / Q000097:blood*; D006801:Humans; D007166:Immunosuppressive Agents / Q000097:blood*; D008213:Lymphocyte Activation; D051379:Mice; D008970:Molecular Weight
D003470:Culture Media; D006131:Growth Inhibitors; D007166:Immunosuppressive Agents; D004247:DNA