The inhibition of human carbonic anhydrase II by some organic compounds.
The inhibition of human carbonic anhydrase II (carbonate hydro-lyase, EC 4.2.1.1) by tetrazole, 1,2,4-triazole, 2-nitrophenol, and chloral hydrate has been investigated. These inhibitors, together with phenol which has been studied previously (Simonsson, I., Jonsson, B.-H. and Lindskog, S. (1982) Biochem. Biophys. Res. Commun. 108, 1406-1412), can be classified in three groups depending upon the kinetic patterns of inhibition of CO2 hydration at pH near 9. The first group, represented by tetrazole and 2-nitrophenol, yields predominantly uncompetitive inhibition under these conditions in analogy with simple, inorganic anions. The second group, represented by 1,2,4-triazole and chloral hydrate gives rise to essentially noncompetitive inhibition patterns, whereas phenol, representing the third group, is a competitive inhibitor of CO2 hydration. These diverse inhibition patterns are discussed in terms of the kinetic mechanism scheme originally proposed by Steiner et al. (Steiner, H., Jonsson, B.-H. and Lindskog, S. (1975) Eur. J. Biochem. 59, 253-259.
D001667:Binding, Competitive; D002257:Carbonic Anhydrase Inhibitors; D002697:Chloral Hydrate / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D009596:Nitrophenols / Q000494:pharmacology; D013777:Tetrazoles / Q000494:pharmacology; D014230:Triazoles / Q000494:pharmacology
D002257:Carbonic Anhydrase Inhibitors; D009596:Nitrophenols; D013777:Tetrazoles; D014230:Triazoles; C045575:1,2,4-triazole; C045574:1H-tetrazole; D002697:Chloral Hydrate; C045573:2-nitrophenol