(6-4)Photoproducts are removed from the DNA of UV-irradiated mammalian cells more efficiently than cyclobutane pyrimidine dimers.
A polyclonal antiserum raised against UV-irradiated DNA can be used to assay cyclobutane pyrimidine dimers and Pyr(6-4)Pyo photoproducts specifically by changing the nature of the 32P-labelled antigen. Pyr(6-4)Pyo photoproducts were removed faster than cyclobutane dimers in UV-irradiated human, hamster and mouse cells. Xeroderma pigmentosum cells from complementation groups A, C and D were deficient in the repair of both lesions.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D006224:Cricetinae; D003503:Cyclobutanes; D004260:DNA Repair / Q000528:radiation effects*; D006801:Humans; D051379:Mice; D009691:Nucleic Acid Denaturation; D010777:Photochemistry; D011740:Pyrimidine Dimers / Q000528:radiation effects*; D011863:Radioimmunoassay; D014466:Ultraviolet Rays; D014983:Xeroderma Pigmentosum / Q000235:genetics
D003503:Cyclobutanes; D011740:Pyrimidine Dimers