The determination of molecular weights of biologically active proteins by cetyltrimethylammonium bromide-polyacrylamide gel electrophoresis.
A novel cetyltrimethylammonium bromide-polyacrylamide gel electrophoresis system which is useful for the separation of native forms of proteins consistent with their molecular weights is reported here. Many proteins examined in this system demonstrated the same association patterns which have been shown by other techniques to exist under nondenaturing conditions. In addition, biological activity could be assayed directly in the gel after electrophoresis. Based on the peculiar characteristics of cetyltrimethylammonium bromide, a possible explanation which may account for the behavior of proteins in this system is presented.
D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D055598:Chemical Phenomena; D002621:Chemistry; D003101:Collodion; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis*; D000644:Quaternary Ammonium Compounds
D002593:Cetrimonium Compounds; D003902:Detergents; D011506:Proteins; D000644:Quaternary Ammonium Compounds; D003101:Collodion; D000077286:Cetrimonium