In vivo 31-P NMR of photoactivated hematoporphyrin derivative in cat brain.
In vivo 31-P nuclear magnetic resonance (NMR) spectroscopy was performed on cat brains injected with hematoporphyrin derivative (HpD). A 2-cm-diam region of the right parietal lobe was photoactivated with red light. The 31-P NMR spectra of the photoactivated hemisphere revealed increased inorganic phosphate and decreased phosphocreatine and adenosine triphosphate (ATP) levels, compared to spectra obtained from the control hemisphere. In the absence of drug, no difference in spectra was observed between the photoradiated and control lobes. Our studies suggest that in vivo 31-P NMR spectroscopy may be used to monitor the effects of phototherapy on tissue high-energy phosphate metabolism.
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002415:Cats; D005260:Female; D006415:Hematoporphyrins / Q000627:therapeutic use*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010759:Phosphorus Isotopes; D010778:Photochemotherapy
D006415:Hematoporphyrins; D010759:Phosphorus Isotopes