[Characterization of specific receptors for estradiol, induction of vitellogenin and its mRNA in the liver of rainbow trout (Salmo gairdnerii)].
In all egg laying vertebrates, synthesis and use of vitellogenin (Vg) are intimately bound to the active phase of reproduction. In the liver of the rainbow trout (Salmo gairdnerii), Vg synthesis is influenced by estradiol (E2) which, we believe, acts through the classical mechanism of steroid hormone action. After binding of the hormone to a soluble specific receptor protein, the estradiol-receptor complex can interact with chromatin and modulate the expression of Vg genes, leading to increased synthesis of specific mRNA and Vg. We show here: (i) the presence of specific oestrogen receptors (dissociation constant KD congruent to 1.5 X 10(-9) M for E2) in the cytosol of the male trout liver. (ii) The male liver, offering, an ideal experimental control of "zero" background, we followed-in the liver of male trout--the kinetics of induction of Vg mRNA by hybridization with Vg cDNA, after E2 stimulation, and (iii) the apparition of Vg in the serum by using an original rocket immuno-electrophoretic technique. The male trout liver vitellogenin model and the original techniques we developed will be very useful to study the influence of endogenous and exogenous factors on the different steps (receptors, transcription, translation) of vitellogenesis regulation.
D000818:Animals; D001667:Binding, Competitive; D002855:Chromatography, Thin Layer; D003907:Dexamethasone / Q000378:metabolism; D004054:Diethylstilbestrol / Q000378:metabolism; D013196:Dihydrotestosterone / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D007122:Immunoelectrophoresis; D007700:Kinetics; D008074:Lipoproteins / Q000235:genetics*; D008297:Male; D011374:Progesterone / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D011959:Receptors, Estradiol / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000378:metabolism*; D014337:Trout; D014818:Vitellogenesis; D014819:Vitellogenins / Q000096:biosynthesis / Q000235:genetics*
D008074:Lipoproteins; D012333:RNA, Messenger; D011959:Receptors, Estradiol; D011960:Receptors, Estrogen; D014819:Vitellogenins; D013196:Dihydrotestosterone; D011374:Progesterone; D004958:Estradiol; D004054:Diethylstilbestrol; D003907:Dexamethasone
Caractérisation de récepteurs spécifiques à l'oestradiol, induction de la vitellogénine et de son mRNA dans le foie de truite arc-en-ci el (Salmo gairdnerii).