[Study of the kinetics of thermoinactivation of lysyl-tRNA-synthetase from the liver of x-irradiated rats].
A study was made of the kinetics of thermoinactivation of lysyl-tRNA-synthetase isolated from rat liver at early times of radiation damage development after the effect of a minimum absolutely lethal X-radiation dose (0.21 C/kg). The thermostability of a dimer form of the enzyme was shown to be higher than that of a monomer. It was established that substrates had a stabilizing effect on the enzyme during thermoinactivation. On the basis of the data obtained from the studies in the kinetic properties of the enzyme and the thermoinactivation a conclusion is made that lysyl-tRNA-synthetase is stabilized during subunit aggregation. The thermostability of the enzyme was decreased by irradiation.
D000604:Amino Acyl-tRNA Synthetases / Q000528:radiation effects*; D000818:Animals; D007700:Kinetics; D008099:Liver / Q000201:enzymology* / Q000528:radiation effects; D008250:Lysine-tRNA Ligase / Q000037:antagonists & inhibitors / Q000528:radiation effects*; D011833:Radiation Injuries, Experimental / Q000201:enzymology*; D051381:Rats; D013379:Substrate Specificity; D013696:Temperature
D000604:Amino Acyl-tRNA Synthetases; D008250:Lysine-tRNA Ligase
Izuchenie kinetiki termoinaktivatsii lizil-tRNK-sintetazy iz pecheni krys pri deĭstvii rentgenovskikh lucheĭ.