[Certain characteristics of the rat liver transketolase].
Modification of the method for transketolase purification in the rat liver was used to reveal the existence of two molecular forms of this enzyme. One of the forms does not react to development of avitaminosis in animals caused by oxythiamine in different doses. The method is developed for isolation of the basic transketolase form in the rat liver with the 50-80% yield of activity. Km values of two sites for coenzyme binding on protein do not depend on the extent of holoenzyme reconstruction from apoenzyme.
D000818:Animals; D001051:Apoenzymes / Q000378:metabolism; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D010119:Oxythiamine / Q000494:pharmacology; D051381:Rats; D014174:Transketolase / Q000302:isolation & purification* / Q000378:metabolism
D001051:Apoenzymes; D007527:Isoenzymes; D010119:Oxythiamine; D014174:Transketolase
Nekototye kharakteristiki transketolazy pecheni krysy.