Mutagenic potential of anticonvulsant diphyenylhydantoin (DPH) on human lymphocytes in vitro.
Diphenylhydantoin (DPH)-treated human peripheral blood cultures were examined for sister chromatid exchange (SCE) frequency and mitotic index. A significantly enhanced SCE frequency in DPH-treated cultures was observed at a 15 micrograms/ml concentration (10-20 micrograms/ml DPH therapeutic range) and above. These results suggest an enhanced SCE response of lymphocytes in the therapeutic blood level of DPH. A significant linear fall in mitotic index was observed with increasing DPH concentrations. It is postulated that even in therapeutic doses DPH possibly affects nucleic acid metabolism.
D002478:Cells, Cultured; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D009153:Mutagens; D010672:Phenytoin / Q000633:toxicity*; D012854:Sister Chromatid Exchange / Q000187:drug effects
D009153:Mutagens; D010672:Phenytoin