Preservation of integrity of the inner mitochondrial membrane after freeze-thawing and freeze-drying.
The results of this paper illustrate that trehalose partially preserves inner mitochondrial membrane integrity after freeze-thawing and freeze-drying with subsequent rehydration in water. The 2,4-dinitrophenol stimulation of ATPase activity was used as a criterion for membrane integrity. The results show that ATPase activity of lyophilized-rehydrated mitochondria was stimulated up to two to three times.
D000818:Animals; D004187:Disaccharides / Q000494:pharmacology*; D005612:Freeze Drying; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000187:drug effects*; D008854:Microscopy, Electron; D008930:Mitochondria, Liver / Q000648:ultrastructure*; D011309:Preservation, Biological / Q000379:methods*; D051381:Rats; D014199:Trehalose / Q000494:pharmacology*
D004187:Disaccharides; D014199:Trehalose