Ascorbic acid determination in serum and aqueous humour by high-performance liquid chromatography.
High-performance liquid chromatography on a Supelcosil LC-HN2 analytical column in weak anion exchange mode has allowed separation of ascorbic acid, in less than 5 min, from other interfering substances in serum and aqueous humour. UV monitoring at 254 nm enables ascorbic acid to be detected at 20 pmol level. A method for determination of ascorbic acid concentration in serum and aqueous humour is described, and values from 10 cataract patients are reported.
D000368:Aged; D001082:Aqueous Humor / Q000032:analysis*; D001205:Ascorbic Acid / Q000032:analysis*; D002386:Cataract / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged
D001205:Ascorbic Acid