Synthesis of a nonacosapeptide (beta-fragment) corresponding to the N-terminal sequence 1-29 of human liver metallothionein II and its heavy metal-binding properties.
A nonacosapeptide (beta-fragment) corresponding to the N-terminal sequence 1-29 of human liver metallothionein II was synthesized by the fragment condensation method. The Cd-binding ability of the beta-fragment was much stronger than that of cysteine as thionein and synthetic alpha-fragment corresponding to the C-terminal sequence 30-61 of human liver metallothionein II. Both the alpha- and beta-fragments bound preferentially to Cu ions rather than Cd ions.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002104:Cadmium / Q000378:metabolism; D002850:Chromatography, Gel; D003300:Copper / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000032:analysis*; D008668:Metallothionein / Q000032:analysis*; D010446:Peptide Fragments / Q000378:metabolism*
D000596:Amino Acids; D010446:Peptide Fragments; D002104:Cadmium; D003300:Copper; D008668:Metallothionein