Binding of manganese in human and rat plasma.
Albumin, transferrin and 'transmanganin' have all been proposed as the major Mn-binding ligand in plasma. The present investigations were initiated in order to resolve these discrepancies. Compared to other metals tested (109 Cd2+, 65Zn2+, 59Fe3+), 54Mn2+ bound poorly to purified albumin. The addition of exogenous albumin to plasma did not result in an increased 54Mn radioactivity associated with this protein. Also, incubation of 65Zn-albumin in the presence of excess Mn2+ (1 mM) did not result in the displacement of Zn from albumin or Mn binding. In contrast to these results, 54Mn was bound to purified transferrin, not as readily as Fe3+, but better than Zn2+ or Cd2+. Saturation of transferrin with Fe3+ (1.6 micrograms Fe/mg) prevented the binding of 54Mn indicating that Mn probably binds to Fe-binding sites on the protein. Polyacrylamide gel electrophoresis further demonstrated the association of 54Mn with transferrin rather than with albumin in both human and rat plasma. The amount of 54Mn radioactivity recovered with transferrin increased as incubation time was increased, probably due to oxidation of Mn2+ to Mn3+. Mn binding to transferrin reached a maximum within 5 and 12 h of incubation. About 50% of 54Mn migrated with transferrin, whereas only 5% was associated with albumin. A significant portion (20-55%) of the 54Mn radioactivity migrated with electrophoretically slow plasma components whose identity was not determined. Possibilities include alpha 2-macroglobulin, heavy gamma-globulins and/or heavy lipoproteins.
D000818:Animals; D001665:Binding Sites; D002104:Cadmium / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007501:Iron / Q000097:blood; D008345:Manganese / Q000097:blood*; D008670:Metals / Q000097:blood*; D011485:Protein Binding; D051381:Rats; D012709:Serum Albumin / Q000378:metabolism; D014168:Transferrin / Q000378:metabolism; D015032:Zinc / Q000097:blood
D008670:Metals; D012709:Serum Albumin; D014168:Transferrin; D002104:Cadmium; D008345:Manganese; D007501:Iron; D015032:Zinc