Receptor binding of 6 alpha-methylprogesterone in mouse kidney.
6 alpha-Methylprogesterone (6MP) is an androgenic progestin that binds to the androgen receptor. However, results from an in vivo study suggested that 6MP was also bound by a second receptor. In the present study, we found that 6MP was bound in kidney cytosol from adrenalectomized/ovariectomized female mice as well as Tfm/Y mice, which lack androgen receptors. 6MP was bound with high affinity (Kd = 1.2 X 10(-8) M) by a binder that was present in 7-8 times greater concentration than the androgen receptor and had the specificity of a glucocorticoid receptor. 6MP was bound with similar specificity in liver cytosol. These data indicate that, despite its androgenic effects, 6MP binds primarily to a glucocorticoid receptor in mouse kidney.
D000818:Animals; D001667:Binding, Competitive; D003593:Cytoplasm / Q000378:metabolism; D003907:Dexamethasone / Q000378:metabolism; D005260:Female; D007668:Kidney / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D011374:Progesterone / Q000031:analogs & derivatives* / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*
D011965:Receptors, Glucocorticoid; D011980:Receptors, Progesterone; D011987:Receptors, Steroid; D011374:Progesterone; D003907:Dexamethasone; C020408:6-alpha-methyl-progesterone