Determination of protein absorption coefficients using a refractive index chromatographic monitor.
Using lysozyme as a primary standard, a refractive index monitor designed for column chromatography was used to determine protein concentration and hence absorption coefficients. The method is non-destructive, requires only small amounts of protein approximately 0.2 mg, and could be adapted for smaller samples.
D002845:Chromatography / Q000379:methods*; D009113:Muramidase; D011506:Proteins / Q000032:analysis*; D012031:Refractometry; D013057:Spectrum Analysis
D011506:Proteins; D009113:Muramidase