The extraction and analysis of quaternary ammonium compounds in biological material by GC and GC/MS.
An injection port pyrolysis method for the analysis of quaternary ammonium compounds is reported. Identification and confirmation of the analyte was accomplished by gas chromatography/mass spectrometry. With minor modifications to pH used and solvent composition, a common ion-pair method of extraction of quaternary ammonium compounds from biological matrices with an iodide counter ion is described. Although the combined extraction-identification methodology was limited to neostigmine, succinylcholine, and paraquat, it should easily be applicable to any of the other compounds examined in this study.
D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D006207:Half-Life; D006801:Humans; D009132:Muscles / Q000378:metabolism; D009388:Neostigmine / Q000097:blood; D010269:Paraquat / Q000652:urine; D000644:Quaternary Ammonium Compounds / Q000032:analysis*; D013390:Succinylcholine / Q000097:blood
D000644:Quaternary Ammonium Compounds; D009388:Neostigmine; D013390:Succinylcholine; D010269:Paraquat