Spinal fluid protein revisited: a reappraisal of the biuret procedure.
In this study our previously described selected method, a biuret procedure with deproteinization, for the determination of spinal fluid protein is thoroughly discussed against the background of the results found with a number of Lowry modifications. The use of various separation techniques, i.e. deproteinization, ultrafiltration and chromatography (HPLC), for protein analysis led to the question as to whether low molecular weight proteins in cerebrospinal fluid play an important role or not with respect to the choice of a selected method for the determination of total protein.
D001738:Biuret Reaction; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D002623:Chemistry Techniques, Analytical; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D014462:Ultrafiltration
D002556:Cerebrospinal Fluid Proteins