Separation of serum proteins on a Fe3+-monohydroxamate adsorbent.
The Fe3+-monohydroxamate adsorbent was tested by chromatography of increasing volumes of serum proteins. The specifically bound proteins were displaced in a stepwise manner by increasing the sodium chloride concentration. The resolved chromatographic fractions were further analysed by gel electrophoresis. A comparison between the properties of Fe3+-monohydroxamate and commercially available adsorbents is presented.
D001798:Blood Proteins / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D003956:Dialysis; D005291:Ferrichrome; D006023:Glycoproteins / Q000302:isolation & purification; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006877:Hydroxamic Acids; D013056:Spectrophotometry, Ultraviolet
D001798:Blood Proteins; D006023:Glycoproteins; D006877:Hydroxamic Acids; D005291:Ferrichrome