[Ultracytochemical study of adenylate cyclase localization in the adenohypophysis of rats].
Using an electron cytochemical method and adenylylimidodiphosphate (AMP--PNP) as substrate, the localization of adenylate cyclase activity was determined in the rat's adenohypophysis. This activity was discovered in the perinuclear space, in the canaliculi of the endoplasmic reticulum and Golgi complex, in mitochondria, on the external surface of the plasma membrane. In sinusoidal capillaries, the reaction product was localized on the plasma membrane, in perinuclear space, endoplasmic reticulum and mitochondria. The addition of isoproterenol and sodium fluoride to the incubation medium led to a rise in adenylate cyclase activity.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000266:Adenylyl Imidodiphosphate / Q000378:metabolism; D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects; D006651:Histocytochemistry; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D008854:Microscopy, Electron; D010903:Pituitary Gland, Anterior / Q000201:enzymology / Q000648:ultrastructure*; D051381:Rats; D012969:Sodium Fluoride / Q000494:pharmacology; D013379:Substrate Specificity
D000266:Adenylyl Imidodiphosphate; D012969:Sodium Fluoride; D000262:Adenylyl Cyclases; D007545:Isoproterenol
Ul'tratsitokhimicheskoe izuchenie lokalizatsii adenilattsiklazy v adenogipofize krys.