Use of guanidine hydrochloride in the purification by reversed-phase high-performance liquid chromatography of thyroxinyl- and triiodothyronylpeptides derived from thyroglobulin.
When peptides containing thyroid hormones are first solubilized in 6 M guanidine hydrochloride they can be perfectly separated by reversed-phase high-performance liquid chromatography on an octadecylsilica column using conventional elution conditions (trifluoroacetic acid-acetonitrile).
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D019791:Guanidine; D006146:Guanidines; D010446:Peptide Fragments / Q000302:isolation & purification*; D013552:Swine; D013954:Thyroglobulin / Q000032:analysis*; D013974:Thyroxine / Q000302:isolation & purification*; D014284:Triiodothyronine / Q000302:isolation & purification*
D006146:Guanidines; D010446:Peptide Fragments; D014284:Triiodothyronine; D013954:Thyroglobulin; D019791:Guanidine; D013974:Thyroxine