[Study of cellular autofluorescence of cultured human cancer cell lines using a fluorescence-activated cell sorter (FACS-IV)].
Quantitative cellular autofluorescence measurement of viable cultured human cancer cells and freshly prepared peripheral blood lymphocytes (PBL) obtained from gastric cancer patients and their cultured PBL with T-cell growth factor (CTC) was undertaken on a FACS-IV. The number of cells with fluorescence and its intensity were significantly higher in CTC than in PBL (p less than 0.001). It can be seen that cancer cell lines have different amounts of autofluorescence in values of individual cells. Although the autofluorescence intensity in cancer cells was generally low, one cell line (SC-1) displayed a higher autofluorescence (7.2%) than the others. Our preliminary results suggest that autofluorescence measurement of cells excited by laser beam for early detection of cancer may be limited, and further induction of highly advanced biological techniques will be needed.
D002460:Cell Line; D002469:Cell Separation / Q000295:instrumentation*; D005434:Flow Cytometry / Q000295:instrumentation*; D005453:Fluorescence; D006801:Humans; D009369:Neoplasms / Q000175:diagnosis / Q000473:pathology*; D013274:Stomach Neoplasms / Q000473:pathology