Muscle fluorometry: a determination of the depth of penetration.
Fluorometric recordings of NADH (nicotinamide adenine dinucleotide) were made on rabbit papillary muscles. The specimens were placed between the UV light source and a small window of the detection stage. As the muscle was moved over the window in a transverse direction, simultaneous measurements could be taken of transmitted UV light and fluorescent light for various thicknesses of tissue. It is concluded that a muscle thickness of 0.65 mm is optimal before absorption of the incident light decreases the fluorescence signal.
D000818:Animals; D005453:Fluorescence; D005470:Fluorometry; D008433:Mathematics; D009132:Muscles / Q000032:analysis / Q000033:anatomy & histology*; D009243:NAD / Q000032:analysis; D010210:Papillary Muscles / Q000032:analysis / Q000033:anatomy & histology; D011817:Rabbits; D012542:Scattering, Radiation
D009243:NAD