Mitogen-like monoclonal anti-actin antibodies.
Monoclonal antibodies (IgM kappa) have been produced to actin isolated electrophoretically from L cell extracts. These monoclonal anti-actin antibodies bind to intact L cells and modulate DNA synthesis and cell proliferation, much like affinity-purified polyclonal rabbit antibody to the same Mr 42,000 actin. In addition, monoclonal antibodies specific for actin from Entamoeba histolytica also bound to and modulated the growth of L cells. A monoclonal antibody directed against a neuroblastoma surface antigen did not produce stimulation of L cells, and the binding activity of anti-actin monoclonal antibody to L cells was removed by absorption with actin covalently coupled to Sepharose. These observations demonstrate the specificity of interaction between the anti-actin monoclonal antibodies and the surface of intact L cells. We conclude that a surface actin-like molecule on the L cell, when bound by specific monoclonal antibody, initiates a stimulatory signal which results in enhanced cellular metabolism.
D000042:Absorption; D000199:Actins / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000502:physiology*; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D002453:Cell Cycle; D002645:Chickens; D004247:DNA / Q000096:biosynthesis; D006825:Hybridomas / Q000276:immunology; D007075:Immunoglobulin M / Q000502:physiology; D007739:L Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens; D011817:Rabbits
D000199:Actins; D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M; D008934:Mitogens; D004247:DNA