Standardization of house dust mite extracts. Liberation of allergenic and antigenic components in relation to extraction conditions.
The spontaneous release of house dust mite components from cultures of Dermatophagoides pteronyssinus into slightly buffered water was studied against time, using both continuous and discontinuous extraction procedures. It was shown that proteins, carbohydrates, IgE binding components and precipitating antigenic components were rapidly released from the house dust mite cultures, reaching a maximal liberation within 1 h of extraction. Repeated extractions of house dust mite cultures (discontinuous extraction) showed an additional release of IgE components but the IgE binding potency declined after successive extractions, while showing increasing release of immunological inactive components. IgE binding to antigens immobilized to polystyrene surfaces (IgE-ELISA) appeared to be less sensitive compared with cyanogen-bromide activated discs (IgE-RAST). It was concluded that extraction procedures of house dust mite cultures with short incubation time of 1 h or less are to be preferred.
D000485:Allergens / Q000276:immunology / Q000302:isolation & purification / Q000592:standards*; D000818:Animals; D000941:Antigens / Q000276:immunology / Q000302:isolation & purification / Q000592:standards; D002457:Cell Extracts / Q000032:analysis / Q000276:immunology / Q000592:standards*; D002478:Cells, Cultured; D004391:Dust / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D007122:Immunoelectrophoresis; D007073:Immunoglobulin E / Q000378:metabolism; D008925:Mites / Q000166:cytology / Q000276:immunology*; D011852:Radioallergosorbent Test; D013997:Time Factors; D014020:Tissue Extracts / Q000592:standards*
D000485:Allergens; D000941:Antigens; D002457:Cell Extracts; D004391:Dust; D014020:Tissue Extracts; D007073:Immunoglobulin E