Studies on activation of benzo[a]pyrene for inhibition of interferon induction.
The induction of interferon-alpha/beta (IFN-alpha/beta) by polyriboinosinic-polyribocytidylic acid (PIC) was inhibited when mouse embryo fibroblasts were pretreated with the carcinogen, benzo[a]pyrene (BP). When BP was added to the cell cultures simultaneously with reduced glutathione (GSH) or beta-naphthoflavone (B-NF), followed 24 hr later by the IFN-alpha/beta inducer, the inhibitory effects of BP on IFN-alpha/beta induction were abrogated. GSH and B-NF both interfere with the activation of BP to its ultimate carcinogenic form. These results suggest that BP must be metabolically activated to inhibit the induction of IFN-alpha/beta.
D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism* / Q000633:toxicity; D001571:Benzoflavones / Q000494:pharmacology; D001711:Biotransformation; D002470:Cell Survival; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000378:metabolism; D005978:Glutathione / Q000494:pharmacology; D007370:Interferon Type I / Q000096:biosynthesis*; D051379:Mice; D008815:Mice, Inbred Strains; D019324:beta-Naphthoflavone
D001571:Benzoflavones; D007370:Interferon Type I; D001564:Benzo(a)pyrene; D019324:beta-Naphthoflavone; D005978:Glutathione