Characterization and purification of lupus antigen La, and RNA-binding protein.
HeLa cell La antigen, an RNA-binding protein, was characterized by using two-dimensional gel electrophoresis. Eight isoelectric forms (pI 6 to 7) were observed, many containing phosphate. An in vitro translation product similar in size and antigenicity was identified. The HeLa cell protein purified by using an assay based on ribonucleoprotein reconstitution with adenovirus VA RNAI also comprised several isoelectric forms.
D000941:Antigens / Q000302:isolation & purification*; D001324:Autoantigens / Q000302:isolation & purification*; D002467:Cell Nucleus / Q000032:analysis; D006367:HeLa Cells / Q000032:analysis; D006801:Humans; D010750:Phosphoproteins / Q000302:isolation & purification; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D012367:RNA, Viral / Q000378:metabolism; D012261:Ribonucleoproteins / Q000378:metabolism; D014157:Transcription Factors / Q000302:isolation & purification*; D000097585:SS-B Antigen
D000941:Antigens; D001324:Autoantigens; D010750:Phosphoproteins; D011494:Protein Kinases; D012367:RNA, Viral; D012261:Ribonucleoproteins; D014157:Transcription Factors; D000097585:SS-B Antigen