Purification of liver and hepatoma membrane proteins by high-performance liquid chromatography.
This paper documents the recovery of selected proteins from hepatic plasma membranes. Initial purification, achieved by a series of stepwise extractions, facilitates the subsequent purification by HPLC. Examples are provided to illustrate the recovery of specific proteins from two Morris hepatoma lines and the liver.
D000818:Animals; D002462:Cell Membrane / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D005615:Freezing; D006023:Glycoproteins / Q000302:isolation & purification; D008099:Liver / Q000032:analysis*; D008114:Liver Neoplasms, Experimental / Q000032:analysis*; D008297:Male; D008565:Membrane Proteins / Q000302:isolation & purification*; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000302:isolation & purification; D012995:Solubility
D006023:Glycoproteins; D008565:Membrane Proteins; D012709:Serum Albumin