Effect of vitamin E on the balance between pro- and antioxidant activity of ascorbic acid in microsomes from rat heart, kidney and liver.
The effect of the vitamin E status of membranes on the balance between pro- and antioxidant activity of ascorbic acid was studied in microsomes from rat heart, kidney and liver. Lipid peroxidation was initiated by 5 microM ferrous ions, in combination with amounts of ascorbic acid ranging from 0-4 mM. Lipid peroxidation was assessed after 1 h of incubation as production of thiobarbituric acid reactive material. It was found that the vitamin E status of the microsomal membranes had little effect on the balance between pro- and antioxidant activity of vitamin C. The sensitivity of the membranes to ferrous ions/ascorbic acid-induced lipid peroxidation, however, was highly dependent on the vitamin E content of the membranes. Vitamin E depletion, in combination with different ascorbic acid concentrations, showed that vitamin E deficiency is not an incontestable model system for enhanced sensitivity to lipid peroxidation in all organs.
D000818:Animals; D000975:Antioxidants; D001205:Ascorbic Acid / Q000494:pharmacology*; D004347:Drug Interactions; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008054:Lipid Peroxides / Q000096:biosynthesis; D008297:Male; D008861:Microsomes / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D014810:Vitamin E / Q000494:pharmacology*; D014811:Vitamin E Deficiency / Q000378:metabolism
D000975:Antioxidants; D008054:Lipid Peroxides; D014810:Vitamin E; D001205:Ascorbic Acid