Use of mitochondrial inhibitors to differentiate kinetic properties of the ATP-dependent Ca2+ uptake system in synaptic membranes.
Synaptosomal membranes accumulate 3-6 times more Ca2+ in the presence of ATP (50-1000 microM) than basal Ca2+ accumulation (-ATP). The location of this Ca2+ accumulation appears to reside on the cytosolic face of the synaptosome since lysed synaptosomes accumulate 4-times more Ca2+ than intact synaptosomes. The inclusion of mitochondrial inhibitors, oligomycin (0.7 micrograms/ml), sodium azide (100 microM) and dinitrophenol (100 microM) differentiate mitochondrial from nonmitochondrial Ca2+ accumulation under conditions that are [Ca2+]- and ATP-dependent. In the presence of low concentrations of ATP (less than 150 microM) and Ca2+ free (2.5 or 6.8 microM), Ca2+ accumulation occurs as one process in both lysed synaptosomal membranes and purified synaptic plasma membranes in the presence and/or absence of MI. When ATP levels are increased (greater than 200 microM), Ca2+ accumulation process remains independent of the presence of mitochondrial inhibitors when Ca2+ free = 2.5 microM. When Ca2+ free is increased to 6.8 microM, mitochondrial inhibitors differentiate mitochondrial from nonmitochondrial accumulation. These studies suggest that optimal conditions for the measurement of Ca2+ accumulating mechanisms in synaptosomal membranes depend on both [Ca2+] and ATP. Use of these assay conditions provide evidence that ATP-dependent Ca2+ uptake may be a viable mechanism for the regulation of synaptosomal Ca2+ levels.
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D001386:Azides / Q000494:pharmacology; D001921:Brain / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002463:Cell Membrane Permeability; D004140:Dinitrophenols / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D009840:Oligomycins / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D019810:Sodium Azide; D013570:Synaptic Membranes / Q000378:metabolism*; D013574:Synaptosomes / Q000378:metabolism
D001386:Azides; D004140:Dinitrophenols; D009840:Oligomycins; D000255:Adenosine Triphosphate; D019810:Sodium Azide; D002118:Calcium