The interaction of calmodulin with the carbocyanine dye (Stains-all).
The dye "Stains-all" combines with calmodulin to yield a series of complex species whose absorption and circular dichroism spectra are sensitive to the presence of Ca2+ or Mg2+. At high dye:calmodulin ratios, the dominant complex formed is characterized by a strong absorption band at 600-650 nm, which is associated with a biphasic circular dichroism band. These spectral features are abolished in the presence of Ca2+.
D001665:Binding Sites; D002118:Calcium; D002147:Calmodulin; D002232:Carbocyanines; D002942:Circular Dichroism; D008274:Magnesium; D011804:Quinolines; D013053:Spectrophotometry
D002147:Calmodulin; D002232:Carbocyanines; D011804:Quinolines; C012040:stains-all; D008274:Magnesium; D002118:Calcium