Increased rate of peripheral nerve regeneration using bioresorbable nerve guides and a laminin-containing gel.
The sciatic nerve of adult mice was transected and proximal and distal nerve stumps were sutured into a nontoxic bioresorbable nerve guide. Nerve guide lumens were either empty or filled with a gel containing 80% laminin and additional extracellular matrix components. Two weeks later cells in the L3 through L5 dorsal root ganglia and the ventral horn of the spinal cord were retrogradely filled with horseradish peroxidase. All animals with the laminin-containing gel but none with empty nerve guides displayed labeled cells. This suggests that the laminin-containing gel significantly hastened axonal regeneration in vivo.
D000042:Absorption; D000818:Animals; D001369:Axons / Q000187:drug effects; D004343:Drug Implants; D005260:Female; D005782:Gels; D007773:Lactates / Q000378:metabolism / Q000494:pharmacology*; D019344:Lactic Acid; D007797:Laminin / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009416:Nerve Regeneration / Q000187:drug effects*; D011091:Polyesters; D011108:Polymers / Q000378:metabolism / Q000494:pharmacology*; D012584:Sciatic Nerve / Q000187:drug effects / Q000502:physiology*
D004343:Drug Implants; D005782:Gels; D007773:Lactates; D007797:Laminin; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)