A rapid method for the extraction and purification of DNA from human leukocytes.
A rapid method for the extraction and purification of DNA from human leukocytes was developed. Crude nucleic acids were obtained by sodium dodecylsulfate (SDS) lysis and potassium acetate precipitation of other cellular material, and the DNA was purified by ribonuclease digestion, diethylaminoethyl (DEAE) cellulose chromatography and ethanol precipitation. DNA obtained by this method is biologically active as reflected by its ability to act as substrate for various nucleases and T4 DNA ligase. The yield was sufficiently high that DNA from less than 1 ml of blood could be used for a number of reactions.
D002848:Chromatography, DEAE-Cellulose; D004247:DNA / Q000302:isolation & purification* / Q000378:metabolism; D011088:DNA Ligases / Q000494:pharmacology; D006801:Humans; D007962:Leukocytes / Q000032:analysis*
D004247:DNA; D011088:DNA Ligases