Plasma determination of 3-methylclonazepam by capillary gas chromatography.
A capillary gas-liquid chromatographic method for the determination of 3-methylclonazepam in plasma was developed. This method involved a single extraction by butyl acetate followed by analysis of the organic extract on a CP-Sil 5 glass capillary column with detection by electron capture. The detection limit was about 0.1 ng/ml, and the inter- and intra-assay precision did not exceed 8% for the concentration range 0.1-6.0 ng/ml. Specificity towards some of the possible metabolites in human plasma was demonstrated. This method was used for the measurement of the pharmacokinetic parameters of 3-methylclonazepam in healthy volunteers after a single intravenous administration of 1 mg, and oral administrations of 1 and 4 mg.
D001570:Benzodiazepinones / Q000097:blood*; D002849:Chromatography, Gas; D004355:Drug Stability; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007700:Kinetics; D012556:Schistosomicides / Q000097:blood*; D002998:Clonazepam / Q000031:analogs & derivatives
D001570:Benzodiazepinones; D012556:Schistosomicides; C020602:meclonazepam; D002998:Clonazepam