Ribonuclease P catalysis differs from ribosomal RNA self-splicing.
Two RNA-catalyzed reactions have been described, the Tetrahymena self-splicing ribosomal RNA and ribonuclease P. The Tetrahymena self-splicing reaction proceeds through a transesterification cascade that is dependent upon nucleophilic attacks by ribose 3'-OH groups. Periodate oxidation of the catalytic (or substrate) RNA, which destroys the nucleophilicity of RNA 3' termini, did not inhibit ribonuclease P activity. Thus, catalysis by ribonuclease P differs from the self-splicing reaction.
D000818:Animals; D002384:Catalysis; D004722:Endoribonucleases / Q000378:metabolism*; D010084:Oxidation-Reduction; D012326:RNA Splicing; D012335:RNA, Ribosomal / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D043262:Ribonuclease P; D013329:Structure-Activity Relationship; D013768:Tetrahymena / Q000502:physiology
D012335:RNA, Ribosomal; D012343:RNA, Transfer; D004722:Endoribonucleases; D043262:Ribonuclease P