Standard lecithin/sphingomyelin and phosphatidylglycerol techniques compared with immunologic slide test.
A commercially available, rapid, technically simple, immunologic slide agglutination test for amniotic fluid (AF) phosphatidylglycerol has been compared with the lecithin/sphingomyelin (L:S) ratio and phosphatidylglycerol determined by thin-layer chromatography. The immunologic test lacks sensitivity when compared with phosphatidylglycerol measured by thin-layer chromatography, and both phosphatidylglycerol measurements are considerably less sensitive than the L:S ratio. The authors' results suggest that the immunologic test is probably useful as a screening test, but all negative results should be immediately followed up with an L:S ratio and phosphatidylglycerol measured by thin-layer chromatography.
D000372:Agglutination Tests / Q000379:methods*; D000653:Amniotic Fluid / Q000032:analysis*; D002855:Chromatography, Thin Layer / Q000379:methods*; D006801:Humans; D010713:Phosphatidylcholines / Q000032:analysis*; D010715:Phosphatidylglycerols / Q000032:analysis*; D013109:Sphingomyelins / Q000032:analysis*
D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D013109:Sphingomyelins