Properties of purified bovine platelet-derived growth factor stimulating proliferation of human and mouse fibroblasts.
Platelet-derived growth factor (PDGF) was isolated from platelets obtained from fresh bovine blood. The platelet lysates were extracted with acid-ethanol, according to the classic procedure that has been applied to the preparation of various protein hormones and transforming growth factors, but not until now to PDGF isolation. Bovine PDGF was further purified by ion-exchange chromatography on DEAE-Sephadex, CM-Sephadex, and finally by molecular sieving on Sephadex G-100. Bovine PDGF has an isoelectric point of 9.45-10.6 and a molecular weight ranging from 28 000 to 31 000. Reduction of bovine PDGF with 2-mercaptoethanol revealed the presence of two polypeptides with molecular weights of approximately 14 000 and 15 000. A simple biologic microassay of cell multiplication-stimulating activity has been developed in our laboratory. Purified bovine PDGF stimulated the proliferation of BALB/c 3T3 or human fibroblasts at minimal concentrations of 0.36-1.5 ng/mL.
D000818:Animals; D001681:Biological Assay; D002417:Cattle; D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007526:Isoelectric Point; D051379:Mice; D008970:Molecular Weight; D010982:Platelet-Derived Growth Factor / Q000302:isolation & purification* / Q000494:pharmacology
D010982:Platelet-Derived Growth Factor