[Action of neurotropic substances on gamma-aminobutyric acid release from brain synaptosomes during K+ depolarization].
The effects of aminazine (0.25 mM), phthoracyzine (0.5 mM), trifluperidole (0.5 mM) and imipramine (0.5 mM) on GABA release from rat brain synaptosomes depolarized with K+ (50 mM) were investigated. Incubation of synaptosomes with aminazine led to a 2-fold and that with phthoracyzine, trifluperidole and imipramine to a 1.5-fold increase in GABA release from synaptosomes as compared with its basic level. The raising of K+ in the incubation medium to 50 mM brought about a 2-fold augmentation of GABA release. Exposure of synaptosomes to drugs and a higher K+ concentration at a time did not change GABA release as compared to its basic level. Introduction into the incubation medium of the Ga-ionophore A23187 together with 50 mM K+ and trifluperidole or with K+ and imipramine led to the same increase in GABA release from synaptosomes as that produced by the psychotropic drugs as regards native synaptosomes. It is assumed that the lack of the influence of the psychotropic drugs under study of GABA release from synaptosomes depolarized with K+ is caused by blockade of synaptic membrane conductibility for Ca2+.
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002746:Chlorpromazine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007099:Imipramine / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D010640:Phenothiazines / Q000494:pharmacology; D011188:Potassium / Q000502:physiology*; D011619:Psychotropic Drugs / Q000494:pharmacology*; D051381:Rats; D013574:Synaptosomes / Q000187:drug effects* / Q000378:metabolism; D014272:Trifluperidol / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D010640:Phenothiazines; D011619:Psychotropic Drugs; C001228:fluoracizine; D005680:gamma-Aminobutyric Acid; D007099:Imipramine; D014272:Trifluperidol; D011188:Potassium; D002118:Calcium; D002746:Chlorpromazine
Deĭstvie neĭrotropnykh veshchestv na vysvobozhdenie gamma-aminomaslianoĭ kisloty iz sinaptosom golovnogo mozga na fone K+-depoliarizatsii.