Trypanosoma musculi: population dynamics of erythrocytes and leukocytes during the course of murine infections.
Cells of the hemocytic and lymphoreticular series located in the blood, bone marrow, spleen, and peritoneal space have been analyzed throughout the course of Trypanosoma musculi infections of intact and splenectomized C3H female mice. Following an early (within 2 days after trypanosome inoculation intraperitoneally) shift of leukocytes from the blood to the peritoneal space, there occurred a more gradual, prolonged infusion of leukocytes into the peritoneal space, the primary site of infection, that continued until the infection was terminated. There was intense cytogeneractive activity in the spleen that included erythrocytes, lymphocytes, myelocytes, and megakaryocytes. The marrow became primarily a site of monocytopoiesis and, to some extent, of lymphopoiesis. During the first 8 days (approximately) of infection, there was a decline in mature erythrocytes in the blood (the well-known anemia) and development of a profound thrombocytopenia. In splenectomized mice, the depletion of these elements continued unabated until the mice died; the marrow of infected, splenectomized mice failed to provide these elements, as was also the case in intact mice. In the peritoneal space, the intense battle between leukocytes and trypanosomes was reflected in a gradual, impressive rise in the number of dead and fatigued cells and, late in infection, in the development of ascites. Both of these abnormal conditions disappeared shortly after cure of the infection. We conclude that infections of mice with T. musculi result in dedication of the entire lymphoreticular system to the generation of cells that are exported to the peritoneal space to combat the major infection the occurs in that locale. This is consistent with the evidence that the belated immune elimination of T. musculi is a cell-mediated (probably antibody-dependent) process. The disruption of the normal histoarchitecture, the shift in the normal proportions of cells and in cells of different degrees of maturity, and probably, a block imposed on precursor cell maturation, account to a large extent for the well-known failure of immune responses commonly associated with trypanosome infections.
D000818:Animals; D001853:Bone Marrow / Q000469:parasitology; D004906:Erythrocyte Count; D004912:Erythrocytes / Q000469:parasitology*; D004920:Erythropoiesis; D005260:Female; D006098:Granulocytes / Q000469:parasitology; D007958:Leukocyte Count; D007962:Leukocytes / Q000469:parasitology*; D008214:Lymphocytes / Q000469:parasitology; D051379:Mice; D008809:Mice, Inbred C3H; D009000:Monocytes / Q000469:parasitology; D010529:Peritoneal Cavity / Q000469:parasitology; D010976:Platelet Count; D013154:Spleen / Q000469:parasitology; D013156:Splenectomy; D014345:Trypanosoma; D014352:Trypanosomiasis / Q000097:blood / Q000469:parasitology*