Action of diethylcarbamazine citrate on protective immunity in rats infected with Nippostrongylus brasiliensis.
Rats made immune to Nippostrongylus brasiliensis and treated with diethylcarbamazine citrate (DEC) orally (250 mg/kg X 6) exhibited significant suppression of functional immunity. Similarly, administration of compound 48/80 (100 micrograms/rat i.p.) made the immune rats susceptible to challenge infection. Treatment of rats, with 22-day infection with compound 48/80, histamine (20 mg/rat, per os), or L-histidine (20 mg/rat, orally s.c.) did not accelerate worm expulsion. A massive complement-dependent adherence of peritoneal cells (1 X 10(8], isolated from immune DEC-treated and untreated rats, to infective larvae (L3) was observed. Likewise, heavy congregation of normal peritoneal cells to larvae was noticed when the cells were incubated with sera obtained from immune, DEC-treated or untreated rats. The rats receiving mesenteric lymph node cells (125 X 10(6) i.v.) or sera (0.5 ml or 1 ml X 3 i.p.), obtained from immune DEC-treated rats and challenged with infective larvae developed 50% more worms than those which received cells or serum from untreated immune donors. DEC appears to cause suppression of functional immunity and worm expulsion is not histamine mediated.
D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D004049:Diethylcarbamazine / Q000494:pharmacology*; D006632:Histamine / Q000494:pharmacology; D006639:Histidine / Q000494:pharmacology; D007104:Immune Adherence Reaction; D007110:Immunity, Active / Q000187:drug effects; D007116:Immunization, Passive; D008297:Male; D009349:Nematode Infections / Q000276:immunology* / Q000469:parasitology; D009559:Nippostrongylus / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D013237:Stereoisomerism; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D000871:Anthelmintics; D003189:p-Methoxy-N-methylphenethylamine; D006639:Histidine; D006632:Histamine; D004049:Diethylcarbamazine