[Identification of influenza virus type A by solid-phase radioimmunological analysis].
High sensitivity and specificity of solid-phase radioimmunoassay (SPRIA) in identification of influenza A (H1N1 and H2N2) viruses in the infected allantoic culture were demonstrated. Mixtures of influenza hyperimmune sera free from antibodies to host cell antigens and antineuraminidase antibodies were used as active test sera. The test serum a-H1N1 consisted of antisera to A/FmI/47, A/Netherlands/36/56, and A/USSR/090/77 strains; it detected practically all tested variants of H1N1 virus isolated in 1947-1982 in allantoic cultures containing virus-specific protein in amounts of 1.4 to 0.7 ng/ml. For the detection of H2N2 subtype viruses (1957-1967), a mixture of antisera to A/Singapore/1/57, A/Leningrad/2/63, A/Gorkiy/62/65, and A/Tokyo/3/67 viruses was used. This test serum could detect H2N2 virus in the allantoic fluid containing 0.7-035 ng/ml of virus protein.
D000818:Animals; D002642:Chick Embryo; D005069:Evaluation Studies as Topic; D006385:Hemagglutination Inhibition Tests; D006389:Hemagglutinins, Viral / Q000032:analysis; D007106:Immune Sera / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000032:analysis; D009980:Influenza A virus / Q000276:immunology / Q000302:isolation & purification*; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D014776:Virus Cultivation
D006389:Hemagglutinins, Viral; D007106:Immune Sera; D007074:Immunoglobulin G
Identifikatsiia virusa grippa tipa A metodom tverdofazovogo radioimmunologicheskogo analiza.