Induction of selective phosphorylation of a fat body protein of Sarcophaga peregrina larvae by 20-hydroxyecdysone.
20-Hydroxyecdysone was shown to induce selective phosphorylation of a fat body protein of Sarcophaga peregrina larvae with a molecular mass of 30 kDa. This phosphorylation was not associated with synthesis of new protein. Fractionation of 32P-labelled fat body by differential centrifugation showed that this protein was mainly present in the membrane-rich fraction, although we could not specify the membrane. Thus, 20-hydroxyecdysone may modify the function of the fat body by inducing phosphorylation of a specific membrane protein.
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D004175:Diptera / Q000187:drug effects / Q000378:metabolism*; D004441:Ecdysterone / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D004640:Emetine / Q000494:pharmacology; D005216:Fat Body / Q000187:drug effects / Q000378:metabolism*; D007814:Larva / Q000378:metabolism; D010766:Phosphorylation; D011506:Proteins / Q000378:metabolism*
D011506:Proteins; D004441:Ecdysterone; D003513:Cycloheximide; D004640:Emetine