A diffusion limited reaction theory for a solid-phase immunoassay.
Theoretical calculations are presented, describing the kinetics of a solid-phase immunoassay where reactants are allowed to diffuse through a gel wedge in the bottom of a circular basin. The analysis is based on the assumption of a diffusion rate limited reaction at the underlaying surface. The influence of initial concentration, incubation time, temperature and the effect of stirring are predicted by the theoretical model. Experimental data are found to follow the theoretical model with parameters consistent with previous values.
D004058:Diffusion; D005782:Gels; D007118:Immunoassay; D008433:Mathematics; D008962:Models, Theoretical; D012107:Research Design; D013696:Temperature
D005782:Gels