Demonstration of two erythrocyte populations in young chickens by counter-current distribution of 59Fe-labelled cells in dextran-poly(ethylene glycol) two-phase systems.
The technique of fractionating cells by a counter-current distribution procedure in dextran-poly(ethylene glycol) biphasic systems was modified by increasing the settling time and decreasing the top/bottom two-phase volume ratio. Two sub-populations of 59Fe-labelled erythrocytes, namely definitive medullar cells and primitive embryonic cells, were present in the blood of young chicks until about day 8 of age. The primitive cells were progressively replaced by definitive red cells as age increased.
D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D002469:Cell Separation; D002645:Chickens / Q000097:blood*; D003377:Countercurrent Distribution; D004912:Erythrocytes / Q000145:classification*; D019855:Ethylene Glycol; D005026:Ethylene Glycols; D007504:Iron Radioisotopes
D005026:Ethylene Glycols; D007504:Iron Radioisotopes; D019855:Ethylene Glycol