Effect of iodoacetate on local cerebral blood flow and glucose metabolism in cats: a double-radionuclide autoradiographic study.
The effect of iodoacetate (IAA), an inhibitor of glycolysis, on local CBF (LCBF) and local CMRglu (LCMRglu) was studied in cats by means of a double-radionuclide autoradiographic method. Artificial CSF containing 5 mM IAA was superfused on the left parietal cortex under a cranial window for 30 min. [14C]2-Deoxyglucose and [123I]iodoantipyrine were injected for the determination of LCMRglu and LCBF, respectively. A marked increase in LCBF, accompanied by a moderate to severe depression of LCMRglu, was observed in the IAA-superfused cortex. This result suggests that LCBF may be closely regulated by the cellular energy state associated with glycolytic activity in brain tissue.
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D002415:Cats; D002560:Cerebrovascular Circulation / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D007461:Iodoacetates / Q000494:pharmacology*; D019807:Iodoacetic Acid; D008297:Male; D011877:Radionuclide Imaging
D007461:Iodoacetates; D005947:Glucose; D019807:Iodoacetic Acid