Haemopoietic modulation in tumour-bearing animals: enhanced progenitor-cell production in femoral marrow.
Altered haematopoiesis in the femoral marrow was observed in mice bearing the Lewis lung carcinoma (LLca). During tumour growth, a marked reduction was observed in the myeloperoxidase-positive cells (granulocytes) of the marrow 7 days after inoculation of the LLca tumour reaching a nadir (17% of control) by day 28. Accompanying this suppression of mature white cells was a gradual expansion of the CFUc-GM compartment followed by an increase in the number of femoral CFUs. Humoral-stimulating activity (HSA) increased through day 14 in the serum of these animals; then returned to control levels by day 28. During this same interval, the more primitive erythroid progenitor (BFUe) compartment expanded to 168% of control, while the more differentiated (CFUe) compartment was reduced (45% of control at day 28). Reductions in both 59Fe-incorporation and erythroblasts/femur confirmed the suppression of erythroid differentiation in marrow during tumour growth. Similar results were observed following the daily injection (188 mg equivalent dose; q 24 hr X 10) of the supernatant prepared from LLca tissue. Marked differences were observed between the response of the spleen and the marrow to the supernatant. The data suggest that the growth of the LLca tumour results in a dissociation of the normal continuity of haematopoietic steady-state differentiation in the marrow of tumour-bearing animals.
D000818:Animals; D001853:Bone Marrow / Q000503:physiopathology; D002277:Carcinoma / Q000503:physiopathology*; D003114:Colony-Forming Units Assay; D004920:Erythropoiesis; D006098:Granulocytes / Q000502:physiology; D006410:Hematopoiesis; D008297:Male; D051379:Mice; D009374:Neoplasms, Experimental / Q000503:physiopathology; D013154:Spleen / Q000503:physiopathology