Estimation of heat production by cultured cells in suspension using semi-automated flow microcalorimetry.
Modifications to a heat conduction flow microcalorimeter are described which allow registration of heat production by cells cultured in suspension. LS cells produced 34 +/- 3 pW per cell. Over an 8.5 h period, cell numbers increased by 9% and heat production per cell by 18%. Oxygen consumption per cell was 0.244 +/- 0.02 mumol min-1 per 10(8) cells and the enthalpy change was -836 kJ/mol O2. An automated pumping system allowed sequential registration of heat production by untreated cells and those exposed to a metabolic inhibitor. The results showed that 0.1 mM 2,4-dinitrophenol caused a greater increase in power (+65% at 1.5 h) than in oxygen consumption (+36%). The opposite occurred in the case of cells treated with 1 mM potassium cyanide, heat dissipation being depressed (-48%) slightly less than oxygen uptake (-52%). The results illustrate the potential of careful calorimetric determinations in studying metabolic events in the growth and division of cells in culture.
D019297:2,4-Dinitrophenol; D000818:Animals; D001322:Autoanalysis; D002151:Calorimetry / Q000379:methods*; D002460:Cell Line; D004140:Dinitrophenols / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D010101:Oxygen Consumption / Q000187:drug effects; D011190:Potassium Cyanide / Q000494:pharmacology; D013816:Thermodynamics
D004140:Dinitrophenols; D011190:Potassium Cyanide; D019297:2,4-Dinitrophenol