Presence or absence of a G1 period in the cell cycle and growth control in a Chinese hamster cell line.
Cells of the V79-8 line grow with little or no G1 period. Serum or isoleucine deprivation results in cell death rather than entry into quiescence. Introduction of a G1 period of 4-5 h by treatment with a mutagen is not sufficient to re-establish the ability to enter quiescence. The experiments described here show that introducing a G1 period by mutation that lowers the rate of protein synthesis does not restore to the cell any competence to arrest in G1 and enter a state of quiescence.
D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media; D007399:Interphase; D007532:Isoleucine / Q000172:deficiency
D003470:Culture Media; D007532:Isoleucine