[Distribution of spontaneous and 8-methoxypsoralen-induced sister chromatid exchanges along the length of the 1st chromosome of Chinese hamster cells].
Distributions of spontaneous, induced by monoadducts and induced by crosslinks sister chromatid exchanges (SCE) along the first chromosome of Chinese hamster cells are nonrandom. All experimental distributions have low frequency of SCE in centromeric and telomeric regions. It can be explained by specific structural organization of the chromosome. However, there are some differences between experimental distributions. Distribution of SCE induced by crosslinks differs from that of spontaneous SCE. Distribution of SCE induced by monoadducts, unlike other distributions, has an increased frequency of exchanges in the q11 region. This region contains several narrow closely disposed G+ bands. It is possible that monoadducts lead to increasing SCE frequency on G+-G- junctions. Distribution of SCE induced by crosslinks resembles random distribution, except centromeric and telomeric regions. These results lead to conclusion that the mechanisms of formation of spontaneous, induced by monoadducts and induced by crosslinks SCE differ from each other.
D000818:Animals; D002478:Cells, Cultured; D002875:Chromosomes / Q000187:drug effects* / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics; D007834:Lasers; D008730:Methoxsalen / Q000494:pharmacology*; D012854:Sister Chromatid Exchange / Q000187:drug effects* / Q000528:radiation effects; D014466:Ultraviolet Rays
D004247:DNA; D008730:Methoxsalen
Raspredelenie spontannykh i indutsirovannykh 8-metoksipsoralenom sestrinskikh khromatidnykh obmenov po dline pervoĭ khromosomy kletok kitaĭskogo khomiachka.