Increased arachidonate incorporation in perfused heart phospholipids from vitamin E-deficient rats.
When perfused with exogenous arachidonic acid (AA), the rat heart incorporates this eicosanoid precursor into the phospholipids. The incorporation of AA into phosphatidylcholine was 6-fold higher than the incorporation of AA into phosphatidylethanolamine. When vitamin E-deficient rat hearts were perfused with AA, there was a marked increase in incorporation of AA into both phosphatidylcholine and phosphatidylethanolamine compared to rats fed with a vitamin E supplemented diet. The result of this study suggests that vitamin E has a regulatory role in phospholipid biosynthesis in the mammalian heart.
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D002250:Carbon Radioisotopes; D004075:Diglycerides / Q000096:biosynthesis; D005227:Fatty Acids / Q000378:metabolism; D006321:Heart / Q000033:anatomy & histology; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009929:Organ Size; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D010714:Phosphatidylethanolamines / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000096:biosynthesis; D014811:Vitamin E Deficiency / Q000378:metabolism*
D001095:Arachidonic Acids; D002250:Carbon Radioisotopes; D004075:Diglycerides; D005227:Fatty Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D014280:Triglycerides