Degradation and covalent cross-linking of glutathione reductase by hemin.
Hemin (ferriprotoporphyrin IX-chloride) can mediate the covalent cross-linking and degradation of yeast glutathione reductase. This reaction requires both NADPH and oxygen suggesting the involvement of a reduced oxygen species in the cross-linking and degradation process. During the course of the reaction the enzymatic activity of glutathione reductase is rapidly destroyed. Implications of these findings for a regulatory role of hemin in cell biology are discussed.
D004591:Electrophoresis, Polyacrylamide Gel; D005980:Glutathione Reductase; D006418:Heme / Q000031:analogs & derivatives*; D006427:Hemin / Q000494:pharmacology*; D009249:NADP / Q000494:pharmacology; D010100:Oxygen / Q000494:pharmacology; D015003:Yeasts / Q000201:enzymology
D006418:Heme; D009249:NADP; D006427:Hemin; D005980:Glutathione Reductase; D010100:Oxygen