A chick embryo fibroblast protein kinase recognizing ribosomal protein S6. Activity increase after serum stimulation.
We sought protein kinase(ser) activity in DEAE-Sephacel chromatography fractions of the 10000 X g supernatants of chick embryo fibroblasts using 40 S ribosomal subunits as kinase substrate, and detected a new S6-recognizing kinase activity. There was one order of magnitude more enzyme activity in chromatography fractions derived from serum-stimulated than from serum-deprived cells. Known protein kinase regulators and a low dose tryptic treatment did not increase the enzyme's activity.
D000818:Animals; D001790:Blood Physiological Phenomena; D002642:Chick Embryo; D002848:Chromatography, DEAE-Cellulose; D005347:Fibroblasts / Q000201:enzymology*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D038601:Ribosomal Protein S6; D012269:Ribosomal Proteins / Q000378:metabolism*; D013379:Substrate Specificity
D038601:Ribosomal Protein S6; D012269:Ribosomal Proteins; D011494:Protein Kinases