Coronary venous perfusion of the ischemic myocardium during acute coronary artery occlusion in isolated rat hearts.
Effects of retrograde coronary venous perfusion on oxygen supply and energy metabolism of ischemic myocardium of the isolated perfused rat heart were examined by means of NADH fluorescence photography. Occlusion of the left coronary artery produced regional ischemia of the left ventricular free wall, as evidenced by the sharply demarcated increase in NADH fluorescence. During ischemia, a narrow area of minimal fluorescence (140 +/- 10 microns), indicating sufficient oxygenation for oxidative phosphorylation, was observed around the epicardial coronary veins in the ischemic lesion. Retrograde perfusion was introduced through the coronary vein (left cardiac vein) that drained off the ischemic area, which resulted in a marked reduction of the area of increased NADH fluorescence in the epicardial surface. In the cross-sectional view, although the myocardium of the entire ischemic area induced by left coronary artery occlusion could be perfused by venous retroperfusion, the effect on reduction of the area of increased NADH fluorescence was seen only in the epicardial half of the myocardium. Retrograde coronary venous perfusion also resulted in a small increase in tension development (P less than 0.05), a decrease in resting tension (P less than 0.01), and partial preservation of myocardial high energy phosphate content (P less than 0.01). We propose that coronary venous retroperfusion improves oxygenation, partially preserves oxidative phosphorylation in the epicardium, and improves contractile function in the ischemic region.
D000208:Acute Disease; D000818:Animals; D001157:Arterial Occlusive Diseases / Q000378:metabolism*; D052160:Benzothiazoles; D003327:Coronary Disease / Q000378:metabolism / Q000503:physiopathology*; D003331:Coronary Vessels / Q000503:physiopathology*; D019793:Fluorescein; D005452:Fluoresceins; D066298:In Vitro Techniques; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D010477:Perfusion; D010781:Photography; D051381:Rats; D011919:Rats, Inbred Strains; D013844:Thiazoles
D052160:Benzothiazoles; D005452:Fluoresceins; D013844:Thiazoles; D009243:NAD; C009462:thioflavin T; D019793:Fluorescein