The interaction of slow-release immunoadjuvants with selected antigens measured in vitro.
A simple, rapid in vitro binding assay for measuring the binding of protein preparations to slow release immunoadjuvants is described. This spectrophotometric method requires no unstable radiolabelled or enzyme reagents and replaces the more tedious and subjective indirect hemagglutination procedure. Therefore, it provides a potential supplement to in vivo animal work in adsorbed vaccine production. The assay was used to study the interaction or complexation behaviour of two adjuvants with tetanus toxoid.
D000276:Adjuvants, Immunologic / Q000378:metabolism*; D017607:Aluminum Compounds; D000941:Antigens / Q000378:metabolism*; D003692:Delayed-Action Preparations; D004168:Diphtheria Toxoid / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010710:Phosphates / Q000378:metabolism; D011485:Protein Binding; D013745:Tetanus Toxoid / Q000276:immunology / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
D000276:Adjuvants, Immunologic; D017607:Aluminum Compounds; D000941:Antigens; D003692:Delayed-Action Preparations; D004168:Diphtheria Toxoid; D010710:Phosphates; D013745:Tetanus Toxoid; D014443:Tyrosine; C045245:stearyl tyrosine; C012714:aluminum phosphate