Separation of dansyl hydrazine-derivatized oligosaccharides by liquid chromatography.
A sensitive method has been developed for the rapid separation of neutral oligosaccharides derivatized with dansyl hydrazine by liquid chromatography on silica gel modified with 1,4-diaminobutane. Malto-oligosaccharides from corn syrup in amounts ranging from 7 to 280 nmol and ovalbumin-derived oligosaccharides in amounts ranging from 0.25 to 250 nmol were easily detectable and gave linear responses over the respective ranges.
D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003619:Dansyl Compounds; D006834:Hydrazines; D009844:Oligosaccharides / Q000032:analysis*; D010047:Ovalbumin / Q000032:analysis; D013050:Spectrometry, Fluorescence
D003619:Dansyl Compounds; D006834:Hydrazines; D009844:Oligosaccharides; C027913:dansyl hydrazine; C019193:maltopentaose; D010047:Ovalbumin