A pasteurized antithrombin III concentrate for clinical use.
A method for large-scale production of a pasteurized antithrombin III (AT III) concentrate for therapeutic use has been adapted from published methods. It includes the following steps: (1) batchwise adsorption onto heparin-Sepharose from plasma depleted of cryoprecipitate and prothrombin complex; (2) chromatographic elution at high salt concentration; (3) pasteurization for 10 h at 60 degrees C in the presence of added citrate ion; (4) desalting on Sephadex G-25, and (5) sterile filtration and freeze-drying. Seven batches prepared in this manner gave a mean yield of 269 U AT III/kg plasma. The product passed all the usual animal safety and pyrogenicity tests and has been used successfully in several courses of treatment of congenital deficiencies.
D000327:Adsorption; D000990:Antithrombin III / Q000302:isolation & purification* / Q000627:therapeutic use; D020152:Antithrombin III Deficiency; D006358:Hot Temperature; D006801:Humans; D012685:Sepharose / Q000031:analogs & derivatives; D013242:Sterilization
C037405:heparin-sepharose; D000990:Antithrombin III; D012685:Sepharose