Induction of histidine decarboxylase activity in the spleen of mice treated with staphylococcal enterotoxin A and demonstration of its non-mast cell origin.
Histidine decarboxylase (HDC) activity increased 13-, 7-, and 2-fold in the spleen, lung and liver, respectively, but not in other tissues of C57BL/6 mice injected i.v. with 50 micrograms/kg of Staphylococcal enterotoxin A (SEA). But even in the spleen, increase in the histamine level was only 1.5 times that of untreated mice. In genetically mast cell-deficient WBB6F1 - W/Wv mice HDC activity in the spleen increased to the same extent as in wild type WBB6F1 - +/+ mice on SEA treatment, but the histamine level in the spleen also increased 20-fold, whereas it increased only 1.4-fold in +/+ mice. These results suggest that the increases in HDC and histamine resulted from interaction of SEA with non-mast cells in tissues.
D000818:Animals; D002262:Carboxy-Lyases / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D004768:Enterotoxins / Q000494:pharmacology*; D005260:Female; D006632:Histamine / Q000378:metabolism; D006640:Histidine Decarboxylase / Q000096:biosynthesis*; D008407:Mast Cells / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000201:enzymology*; D013997:Time Factors
D004768:Enterotoxins; C026066:enterotoxin A, Staphylococcal; D006632:Histamine; D002262:Carboxy-Lyases; D006640:Histidine Decarboxylase