Phospholipids of normal and experimentally injured spinal cord of the miniature pig.
Experimental spinal cord trauma was produced in 3-month-old SS-1 miniature pigs by dropping a 25 g weight from a height of 20 cm upon the exposed spinal cord. The histological lesion consisted of edema and hemorrhage. Phospholipid concentration and composition, cholesterol concentration and phospholipid fatty acid composition were determined in whole spinal cord 3 hours after injury, and in spinal cord myelin 5 hours after injury. Three hours after injury phospholipid and cholesterol concentration were decreased by about 14% in the whole spinal cord. Trauma had no effect on the phospholipid composition of whole spinal cord and myelin. Fatty acid composition of myelin also did not change after injury, and changed very slightly in the whole spinal cord. It is concluded that edema following spinal cord trauma is much more extensive than previously assumed. Furthermore, peroxidation of membrane lipid fatty acids does not appear to be a significant factor in spinal cord pathology 3 hours after injury.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002784:Cholesterol / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D009186:Myelin Sheath / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*; D013116:Spinal Cord / Q000032:analysis*; D013119:Spinal Cord Injuries / Q000378:metabolism*; D013552:Swine; D013556:Swine, Miniature
D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D010743:Phospholipids; D002784:Cholesterol