Response to immunization after partial and total splenectomy.
Survival after infection from Streptococcus pneumoniae in both animals and man is influenced by the amount of splenic tissue. We investigated the effect of differences in splenic weight upon the antibody response to immunization and the effect of immunization upon survival after pneumococcal challenge. Young Sprague-Dawley rats had either sham operation, hemisplenectomy, splenectomy with splenic autotransplantation, or total splenectomy. Nine weeks later, rats were immunized with a heat- and formalin-killed type-specific pneumococcal vaccine. Antibody response measured by radioimmunoassay was similar in all operative groups and was significantly higher than in nonimmune rats (P less than 0.01). Splenic weight was less after hemisplenectomy or autotransplantation than in sham-operated animals (P less than 0.01). Immunization improved survival after live pneumococcal challenge in rats that had autotransplantation and total splenectomy (P less than 0.001). Our results demonstrate that splenic weight does not affect the antibody response to pneumococcal immunization in rats. Immunization improves survival after bacterial challenge in susceptible animals and minimizes the detrimental effect of reduction in splenic mass.
D000818:Animals; D000917:Antibody Formation; D005260:Female; D007114:Immunization; D009929:Organ Size; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000033:anatomy & histology / Q000276:immunology; D013156:Splenectomy; D013290:Streptococcal Infections / Q000517:prevention & control; D013296:Streptococcus pneumoniae / Q000276:immunology; D013997:Time Factors