[Determination of the genetic polymorphism of the 3d complement component (C'3) by vertical polyacrylamide gel electrophoresis].
A variant of the vertical polyacrylamide gel electrophoresis method, hitherto not applied in determination of C'3, is used. The method allows simultaneous determination of the C'3 phenotypes and other serum proteins in human blood. In this paper, the influence of aging of the serum samples and of the Tf polymorphism on the C'3 electrophoregram is discussed. The gene frequencies in Mongolian population (MPR) are: C'3S = 0.9605, C'3F = 0.0375, and the rare variants C'3Srare = 0.0013, C'3Frare = 0.0007; in Chukchi these are 1.0, 0.0, 0.0, respectively; in Eskimos - 0.9556, 0.0222, 0.0111; Evenki - 0.9359, 0.0641, 0.0; Yakuts - 0.9091, 0.0909, 0.0; Komi-Zyrians - 0.8507, 0.1493, 0.0; Komi-Permiyak - 0.8176, 0.1698, 0.0068.
D000483:Alleles; D044466:Asian People; D003176:Complement C3 / Q000032:analysis / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005006:Ethnicity; D005787:Gene Frequency; D006801:Humans; D008986:Mongolia; D010641:Phenotype; D011110:Polymorphism, Genetic; D012800:Siberia; D014168:Transferrin / Q000032:analysis / Q000235:genetics
D003176:Complement C3; D014168:Transferrin
Opredelenie geneticheskogo polimorfizma tret'ego komponenta komplementa (C'3) metodom vertikal'nogo élektroforeza v poliakrilamidnom gele.