[Study of the protective effect of phosphocreatine on the ischemic myocardium during cardioplegia using the P-31 NMR method].
Using 31P-NMR technique it was shown that exogenous phosphocreatine (10 mM) added in the cardioplegic solution provided higher levels of recovery of intracellular ATP (approx. 60% vs. 26% in the control) and phosphocreatine (90% vs. 43%) in perfused rat hearts after 35 min of total ischemia. Simultaneously significantly higher levels of contractile recovery (90% vs. 35%) and a three-fold decrease in creatine kinase release into the perfusate were observed. These effects of exogenous phosphocreatine can be related to either intracellular action of this compound or its interaction with cellular membrane.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D005500:Follow-Up Studies; D006321:Heart / Q000187:drug effects*; D006324:Heart Arrest, Induced; D009682:Magnetic Resonance Spectroscopy; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure; D010725:Phosphocreatine / Q000378:metabolism / Q000494:pharmacology*; D010758:Phosphorus; D051381:Rats; D013997:Time Factors
D010725:Phosphocreatine; D010758:Phosphorus; D000255:Adenosine Triphosphate
Issledovanie metodom 31P-IaMR zashchitnogo deĭstviia fosfokreatina na ishemizirovannyĭ miokard v usloviiakh kardioplegii.