Characterization of blood group active glycopeptides derived from porcine kidneys.
Sialoglycopeptide fractions were prepared from the pronase digest of porcine kidneys by DEAE-Sephadex A-25 column chromatography and gel-filtration through Sephadex G-100. Their chemical compositions and large molecular size suggested that these glycopeptides were derived from mucin-type glycoprotein(s). The results of the beta-elimination reaction indicated that they have the O-glycosidic linkages between N-acetylgalactosamine and serine/threonine. The glycopeptides exhibited blood group A and H activities. The present study revealed that the porcine kidney contains the blood group antigens of glycoprotein nature.
D000017:ABO Blood-Group System; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004588:Electrophoresis, Cellulose Acetate; D006020:Glycopeptides / Q000032:analysis*; D007668:Kidney / Q000032:analysis*; D011402:Pronase / Q000378:metabolism; D013552:Swine
D000017:ABO Blood-Group System; D000596:Amino Acids; D002241:Carbohydrates; D006020:Glycopeptides; D011402:Pronase