Concentration and purification of influenza virus from allantoic fluid.
A simple procedure which enables the concentration and purification of influenza virus, using an angular rotor, is described. Virus is concentrated over a sucrose step gradient. The same gradients are reused and volumes up to 4 liters are concentrated in 1 day. The concentrated virus is further purified by a simplified density-gradient technique. No host cell protein is detectable in the final product. The technique offers a broad application potential for concentrating and purifying other viruses.
D000482:Allantois / Q000382:microbiology*; D000818:Animals; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D005321:Extraembryonic Membranes / Q000382:microbiology*; D006386:Hemagglutination Tests; D009975:Orthomyxoviridae / Q000302:isolation & purification*; D014776:Virus Cultivation