Regulation of mitochondrial malate dehydrogenase: kinetic modulation independent of subunit interaction.
Porcine heart mitochondrial malate dehydrogenase (EC 1.1.1.37), a dimeric enzyme of Mr = 70,000, is both allosterically activated and inhibited by citrate. Using an affinity elution procedure based upon citrate binding to malate dehydrogenase, the isolation of pure heterodimer (a dimeric species with one active subunit and one iodoacetamide-inactivated subunit) has been achieved. Investigations utilizing this heterodimer in conjunction with resin-bound monomers of malate dehydrogenase have allowed the formulation of a definite conclusion concerning the role of subunit interactions in catalysis and regulation of this enzyme. The citrate kinetic effects, oxaloacetate inhibition, malate activation, and the effects of 2-thenoyl-trifluoroacetone (TTFA) are shown to be independent of interaction between catalytically active subunits. Previous kinetic data thought to support a reciprocating catalytic mechanism for this enzyme may be reinterpreted upon closer analysis in relation to an allosteric, conformationally specific binding model for malate dehydrogenase.
D000494:Allosteric Regulation / Q000187:drug effects; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002951:Citrates / Q000378:metabolism; D003956:Dialysis; D004789:Enzyme Activation / Q000187:drug effects; D007460:Iodoacetamide / Q000494:pharmacology; D007700:Kinetics; D008291:Malate Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008293:Malates / Q000494:pharmacology; D008929:Mitochondria, Heart / Q000201:enzymology*; D010071:Oxaloacetates / Q000494:pharmacology; D013552:Swine; D013804:Thenoyltrifluoroacetone / Q000494:pharmacology
D002951:Citrates; D008293:Malates; D010071:Oxaloacetates; D013804:Thenoyltrifluoroacetone; C030298:malic acid; D008291:Malate Dehydrogenase; D007460:Iodoacetamide