G0 chromosomal radiosensitivity in ataxia telangiectasia lymphocytes.
Contrary to an earlier report, peripheral lymphocytes from 4 AT patients were not found to exhibit higher yields of unequivocal chromosome type aberrations following irradiation in the G0 phase of the cell cycle, providing that only first post-irradiation metaphases were included in the samples (ensured by 5-bromodeoxyuridine (BrdU) incorporation and differential fluorescence or Giemsa staining). We were able, however, to confirm the earlier-reported increase in chromatid-type aberrations in the G0-irradiated cells. AT lymphocytes were found to experience more cell-cycle delay following G0 irradiation than normal cells. These observations appear consistent with the damaged base excision DNA-repair defect reported for AT cells.
D001260:Ataxia Telangiectasia / Q000235:genetics* / Q000473:pathology; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000528:radiation effects*; D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D007399:Interphase / Q000528:radiation effects*; D008214:Lymphocytes / Q000528:radiation effects