Glutamic oxaloacetic transaminase released from chicken spermatozoa during freeze-thaw procedures.
The feasibility of using glutamic oxaloacetic transaminase (GOT) released from chicken spermatozoa for evaluation of freeze-thaw damage and the optimum concentration of glycerol on the basis of GOT release were examined. There was a highly significant correlation (-.88) between sperm motility in media containing various concentrations of glycerol and GOT activity in extracellular media after freeze-thawing. This indicates that GOT activity in the supernatant of frozen-thawed semen may be useful as an indicator for frozen-thawed injury of chicken spermatozoa. The amounts of GOT released from spermatozoa after 20 min of glycerol equilibration at 5 C gradually increased from 1 to 18% glycerol. The GOT activity in the extracellular medium after freeze-thawing was lowest at 10% glycerol. To estimate GOT leakage from spermatozoa during the freeze process, except glycerol equilibration, the amounts of GOT just before freezing were subtracted from the amounts of GOT after freeze-thawing. The minimum difference between GOT leakage before and after freeze-thawing was shown to be at 16% glycerol.
D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism*; D002645:Chickens; D003470:Culture Media; D005615:Freezing; D005990:Glycerol / Q000494:pharmacology; D008297:Male; D012662:Semen Preservation / Q000662:veterinary*; D013081:Sperm Motility; D013094:Spermatozoa / Q000201:enzymology*
D003470:Culture Media; D001219:Aspartate Aminotransferases; D005990:Glycerol