Quantitative analysis of two-dimensional electrophoretograms using shape-fitting.
This paper describes an automatic quantitative analysis of protein using two-dimensional electrophoresis. We made 2-D electrophoretograms with index spots of some proteins whose mass is known. First, we cut out the protein spots from the background. Then we detected connected regions which may correspond to spot regions and measured their mass as the sum of the gray levels in the region. For confluent spots, we propose a method of separating them by shape-fitting, and examine this method by comparing it with the spot-dividing method.
D001699:Biometry; D003201:Computers; D004591:Electrophoresis, Polyacrylamide Gel; D011506:Proteins / Q000032:analysis*
D011506:Proteins