Immunological analysis of eukaryotic transcription factor S-II in a HeLa cell lysate.
Previous experiments using antibody suggested that a stimulatory protein of RNA polymerase II termed S-II is an essential component of accurate transcription in a HeLa cell lysate (1). In this work a radioimmunoassay system for S-II was developed and it was demonstrated that S-II and RNA polymerase II in a HeLa cell lysate could be separated by sucrose density gradient centrifugation. This suggested that S-II and RNA polymerase II do not exist as a complex in the HeLa cell lysate. Probably, S-II is integrated into the initiation complex when appropriate template DNA and nucleoside triphosphates are added to the lysate.
D002499:Centrifugation, Density Gradient; D006367:HeLa Cells / Q000032:analysis*; D006801:Humans; D009363:Neoplasm Proteins / Q000032:analysis*; D012319:RNA Polymerase II / Q000032:analysis*; D011863:Radioimmunoassay; D014157:Transcription Factors / Q000032:analysis*; D035165:Transcription Factors, General; D035602:Transcriptional Elongation Factors
D009363:Neoplasm Proteins; D014157:Transcription Factors; D035165:Transcription Factors, General; D035602:Transcriptional Elongation Factors; C018648:transcription factor S-II; D012319:RNA Polymerase II