Standardization of a hyphal inoculum of aspergilli for amphotericin B susceptibility testing.
Standardized, evenly dispersed hyphal suspensions served as the inoculum in a microtiter technique for amphotericin B antifungal susceptibility testing. Preliminary testing with six strains of Aspergillus fumigatus and A. flavus produced consistent and reproducible results at 30 degrees C over 24 h. The observed amphotericin B MICs required for hyphae (0.3 to 0.6 microgram/ml) were comparable to MICs required for conidia (0.16 to 0.6 microgram/ml). The results were evaluated and compared with previously published information.
D000666:Amphotericin B / Q000494:pharmacology*; D001230:Aspergillus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests / Q000592:standards; D013997:Time Factors
D000666:Amphotericin B