The cellular response of sheep to challenge with cultivated allogeneic tumor cells derived from naturally occurring ovine squamous cell carcinomata.
The ability of three cultivated epithelial tumor cell lines, derived from naturally occurring ovine squamous cell carcinomata, to stimulate a response in normal allogeneic sheep, was investigated. All three chosen cell lines failed to stimulate a response in allogeneic lymphocytes in vitro. In two of the tumors, it was shown that the lack of stimulation could be due to a suppressive effect by the tumor cells on lymphocytes. In vivo, two of the cell lines stimulated a marked cellular response which was associated with the production of high levels of antibody and which induced cytotoxic cell formation. The remaining cell line stimulated only a marginal response. No suppressive activity of the tumor cells was detected in allogeneic sheep. The significance of the response obtained to challenge with tumor cells, as compared to challenge with allogeneic lymphocytes from the tumor cell donor, is also discussed.
D000818:Animals; D000917:Antibody Formation; D000920:Antibody-Dependent Cell Cytotoxicity; D002294:Carcinoma, Squamous Cell / Q000276:immunology* / Q000662:veterinary; D003165:Complement System Proteins / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D007111:Immunity, Cellular; D008213:Lymphocyte Activation; D012756:Sheep / Q000276:immunology; D012757:Sheep Diseases / Q000276:immunology*
D003165:Complement System Proteins