Inhibition of carbonic anhydrase in dog plasma.
Using a pH stat method, we measured the activity of carbonic anhydrase (CA) from dog erythrocytes in the presence of various amounts of dog plasma. A plasma factor appeared to be able to inhibit about 86% of the total CA activity, corresponding to the relative activity of CA II. Naiodoacetate was shown to inhibit the total CA activity up to about 13%, corresponding to the relative activity of CA I. C1- inhibited the total CA activity up to about 20%, presumably mainly through its strong influence on Ca I. It is concluded that with a degree of haemolysis of up to 3%, no appreciable plasma CA activity will occur.
D000818:Animals; D002257:Carbonic Anhydrase Inhibitors / Q000097:blood* / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology; D004285:Dogs; D005459:Fluorides / Q000494:pharmacology; D006461:Hemolysis; D007461:Iodoacetates / Q000494:pharmacology; D019807:Iodoacetic Acid
D002257:Carbonic Anhydrase Inhibitors; D002712:Chlorides; D007461:Iodoacetates; D005459:Fluorides; D019807:Iodoacetic Acid