Quantitative determination of betamethasone and its major metabolite in equine urine by micro-liquid chromatography-mass spectrometry.
Micro-liquid chromatography-mass spectrometry (micro-LC-MS) was utilized to quantitatively determine betamethasone and its major unconjugated metabolite, 6 beta-hydroxybetamethasone, in equine plasma and urine. The advantage of micro-LC-MS over conventional gas chromatography-mass spectrometry in corticosteroid determination is illustrated and the reliable, steadfast nature of micro-LC-MS is demonstrated through example.
D000818:Animals; D001623:Betamethasone / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine*; D002853:Chromatography, Liquid / Q000379:methods; D002855:Chromatography, Thin Layer; D006736:Horses / Q000097:blood / Q000652:urine*; D013058:Mass Spectrometry / Q000379:methods; D010949:Plasma / Q000032:analysis
C031217:6-hydroxybetamethasone; D001623:Betamethasone