Graphical method for analysing stathmokinetic data.
The accumulation of cells in G2M measured flow cytometrically follows a straight line when plotted against time in colcemid with the transform ln (1 + fG2M), where fG2M is the fraction of cells in the G2M peak. Extending this function to the value, ln 2, will locate a point B. Drawing a line from point B to ln 1 perpendicular to the abscissa, will locate point A at the initial value of ln (1 + fG2M). A line from ln 2 at t = 0 through point A will intersect the abscissa at the cell doubling time. Application of this method to the depletion of G1 cells is also possible.
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured; D006224:Cricetinae; D003703:Demecolcine / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D007399:Interphase / Q000187:drug effects; D008938:Mitosis / Q000187:drug effects
D003703:Demecolcine