Chemical and histochemical studies of normal and diseased gastrointestinal tract. IV. A comparison of histochemical and chemical methods for the estimation of side chain O-acylated sialic acids.
Statistically significant correlations were obtained between a chemical assay for the proportion of colonic epithelial glycoprotein sialic acids with side chain O-acyl substituents and two histochemical methods, the PBT-KOH-PAS sequence (rs = 0.7485 for N = 31, P = 0.01, one-sided test) and the PAPT-KOH-Bh-PAS procedure (rs = 0.7024 for N = 34). A positive correlation (rs = 0.8654 for N = 30, P = 0.01) was also obtained between the results of the two histochemical procedures. It is concluded that, on average, histochemical observations are a reliable semiquantitative comparative method for the estimation of side chain O-acetylated sialic acids.
D003106:Colon / Q000032:analysis*; D003108:Colonic Diseases / Q000378:metabolism*; D006651:Histocytochemistry; D006801:Humans; D012794:Sialic Acids / Q000032:analysis*
D012794:Sialic Acids