Characterization of a mitochondrial protein binding to single-stranded DNA.
A DNA-binding protein from Xenopus laevis oocyte mitochondria which has been found associated with the D-loop also shows a strong preference for single-stranded DNA. The binding to polynucleotides is dependent on the base composition, but no sequence specificity was found. This protein, called mtSSB, binds tightly and cooperatively to single-stranded DNA. By its amino-acid composition and its binding properties it appears to be similar to the single-stranded DNA-binding proteins found in prokaryotes.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005260:Female; D008928:Mitochondria / Q000032:analysis*; D009690:Nucleic Acid Conformation; D009865:Oocytes / Q000032:analysis; D013379:Substrate Specificity; D014982:Xenopus laevis
D000596:Amino Acids; D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins