A field method for the procurement of infective larvae of Onchocerca volvulus.
Natural vectors of onchocerciasis (S. yahense) were collected. Ninety-eight were intrathoracically inoculated with 30 freshly obtained skin-dwelling microfilariae. Of the 82 flies dissected, 56 were infected and 28 harbored infective larvae. A total of 193 infective O. volvulus larvae were obtained. The mean infective worm burden was 6.9.
D006801:Humans; D007303:Insect Vectors / Q000469:parasitology; D007814:Larva; D008842:Microfilariae; D009854:Onchocerca / Q000302:isolation & purification* / Q000502:physiology; D009855:Onchocerciasis / Q000469:parasitology; D012843:Simuliidae / Q000469:parasitology