An ultrastructural investigation of Leishmania donovani infection in genetically resistant and susceptible mouse strains.
Natural resistance to the growth of Leishmania donovani in mice is controlled by a gene (Lsh) which is expressed, in an unknown fashion, in macrophages. Early net growth rate of the parasite is much higher in mice strains bearing the susceptible allele (Lshs) than in resistant (Lshr) mice. Intracellular events occurring in the Kupffer cells during this period have been studied at the ultrastructural level. It was found that the number of dividing amastigotes per thin section of infected cell was approximately 10-fold greater in susceptible (B10.A SgSn) than in resistant (A/J) strains of mice, both 7 and 14 days following infection. These findings support the hypothesis that high natural resistance to leishmaniasis (Lshr) is expressed as a microbistatic effect, exerted within the parasitized macrophage of the host.
D000818:Animals; D004198:Disease Susceptibility; D007728:Kupffer Cells / Q000473:pathology* / Q000648:ultrastructure; D007898:Leishmaniasis, Visceral / Q000473:pathology*; D008247:Lysosomes / Q000648:ultrastructure; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics; D008854:Microscopy, Electron