Induction of spheroplasts in Capnocytophaga ochracea.
A gentle technique for preparing spheroplasts of Capnocytophaga ochracea strain 25 is described. Cells in the exponential phase were washed with 1.0 M-NaCl, agitated in 1.0 M-NaCl for 2 h at 30 degrees C and exposed to lysozyme in a Tris/salts buffer, pH 7.0. This procedure resulted in 98% spheroplast formation with complete removal of the peptidoglycan layer as detected by both phase-contrast and electron microscopy in combination with chemical analysis.
D002206:Capnocytophaga / Q000032:analysis* / Q000648:ultrastructure; D041964:Cytophagaceae / Q000032:analysis*; D008722:Methods; D008854:Microscopy, Electron; D013104:Spheroplasts / Q000302:isolation & purification*