Determination of the serum concentration of spironolactone and its metabolites by high-performance liquid chromatography.
A simple and rapid high-performance liquid chromatographic assay is described for the simultaneous determination in serum of the aldosterone antagonist spironolactone and its metabolites 7 alpha-thiomethylspirolactone, 6 beta-hydroxy-7 alpha-thiomethylspirolactone and canrenone. Ultraviolet absorption at 240 nm was used to detect the different compounds after elution on a normal-phase column. Endogenous serum substances did not interfere with the assay. This method provides a convenient tool in pharmacokinetic studies of spironolactone, in contrast to previously reported aspecific fluorimetric assays or time-consuming thin-layer chromatographic analyses of radioactive biological material.
D002192:Canrenone / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013056:Spectrophotometry, Ultraviolet; D013148:Spironolactone / Q000031:analogs & derivatives / Q000097:blood*
D013148:Spironolactone; C046564:7-thiomethylspirolactone; C046565:6-hydroxy-7-thiomethylspirolactone; D002192:Canrenone