A method for isolation of cytoplasmic RNA from a slime mold, Physarum polycephalum.
A procedure for fast and simple preparation of cytoplasmic ribonucleic acid from Physarum polycephalum microplasmodia is described. Microplasmodia are homogenized in a high-magnesium-high-ethylene glycol bis(beta-aminoethyl ether) N,N'-tetraacetic acid buffer and nuclei are pelleted. The supernatant is extracted with sodium dodecyl sulfate-phenol-chloroform and crude RNA is precipitated. This is further purified by selective ethanol precipitation from 6 M guanidinum hydrochloride. This RNA preparation is suitable for direct use in hybridization studies.
D002458:Cell Fractionation / Q000379:methods; D003593:Cytoplasm / Q000032:analysis; D010804:Physarum / Q000032:analysis*; D012331:RNA, Fungal / Q000302:isolation & purification*; D012995:Solubility
D012331:RNA, Fungal