title: [Evans blue as an adjuvant for enhancing delayed hypersensitivity in an experiment].
abstract: A modified method has been elaborated for induction of delayed type hypersensitivity (DH) in CBA mice with the use of Evans blue (EB) as adjuvant. This model permitted studying the mechanism of DH development, establishing the dependence of DH on the dose of EB, the dose and type of protein antigen, and realizing passive transfer of DH with the aid of splenocytes from active-synthesized mice. EB is a convenient and effective adjuvant for induction and study of the mechanism of DH development.
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000941:Antigens / Q000008:administration & dosage; D001391:Azo Compounds / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004306:Dose-Response Relationship, Immunologic; D004342:Drug Hypersensitivity / Q000276:immunology*; D005070:Evans Blue / Q000494:pharmacology*; D005260:Female; D006968:Hypersensitivity, Delayed / Q000139:chemically induced* / Q000276:immunology; D007114:Immunization / Q000379:methods; D007116:Immunization, Passive; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D013997:Time Factors
chemical_list: D000276:Adjuvants, Immunologic; D000941:Antigens; D001391:Azo Compounds; D005070:Evans Blue
vernacular_title: Evans siniĭ kak ad''iuvant dlia polucheniia povyshennoĭ chuvstvitel'nosti zamedlennogo tipa v éksperimente.