Lack of effect of GABA on [3H]leucine incorporation into a rat oviduct ribosomal system.
A ribosomal system for [3H]leucine incorporation was isolated from the rat oviduct in order to study the possible effect of GABA on [3H]leucine incorporation during the estrous cycle. The system showed an absolute requirement for Mg2+ and about 50% dependence on an energy source. Optimal [3H]leucine incorporation occurred under 3-6 mM Mg2+ and 100 mM K+ and was higher in diestrous-1 than in estrous or proestrous. GABA (10 mM) had no effect on [3H]leucine incorporation in any of the three estrous phases studied.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D004734:Energy Metabolism; D004971:Estrus; D005187:Fallopian Tubes / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D007930:Leucine / Q000096:biosynthesis*; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D012270:Ribosomes / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
D000596:Amino Acids; D005680:gamma-Aminobutyric Acid; D007930:Leucine