Isolation and purification of cardiolipin from beef heart.
A simple and efficient procedure is described for the preparation of cardiolipin sodium salt from beef heart. A crude phospholipid fraction is isolated by chloroform-methanol extraction of the homogenized tissue, followed by acetone precipitation and reprecipitation in 4% aqueous CaCl2-methanol. Cardiolipin is separated from the calcium salts of the acidic phospholipids by partition column chromatography on silica gel (Polygosil 60-63100) using 2-propanol-cyclohexane-water 50:43:7 (v/v/v) as eluent. Further purification of the cardiolipin is achieved by high performance liquid chromatography of the calcium salt on silica gel (Lichrosorb Si 60-5) with a neutral eluent (2-propanol-cyclohexane-water 45:50:5 (v/v/v], followed by quantitative conversion to the sodium salt. The yield of this procedure is 1.5-2.1 g of pure 99% sodium salt of cardiolipin per kg of moist ventricular tissue.
D000818:Animals; D002308:Cardiolipins / Q000302:isolation & purification*; D002417:Cattle; D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000032:analysis; D009206:Myocardium / Q000032:analysis*; D010743:Phospholipids / Q000302:isolation & purification; D012964:Sodium
D002308:Cardiolipins; D005227:Fatty Acids; D010743:Phospholipids; D012964:Sodium