Morphometric study of the interphase nucleus in some radiosensitive and radioresistant mammalian cells.
The radiosensitive cell populations, such as resting lymphocytes from thymus, spleen, lymph node and blood, have much smaller nuclei (Vn (nuclear volume) approximately 20 to 70 microns3) compared to radioresistant G0 cells from non-lymphoid tissues (liver, kidney, brain, heart; Vn approximately 75 to 2700 microns3). It is suggested that radiation-induced disorganization of nuclear structures and cell pycnosis (interphase death) are promoted in G0 lymphocytes because in normal physiological conditions their nuclei assume a higher degree of chromatin condensation. In contrast, dispersion of chromatin into larger nuclear volumes, such as those of most non-lymphoid G0 cells, may hinder or delay radiation-induced cell death.
D000818:Animals; D002467:Cell Nucleus / Q000528:radiation effects*; D002470:Cell Survival / Q000528:radiation effects; D002843:Chromatin / Q000528:radiation effects; D006224:Cricetinae; D006801:Humans; D007399:Interphase; D007620:Karyometry; D008214:Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure; D051379:Mice; D011817:Rabbits; D011836:Radiation Tolerance; D051381:Rats
D002843:Chromatin