Synthesis of (+) and (-) RNA molecules of potato spindle tuber viroid (PSTV) in isolated nuclei and its impairment by transcription inhibitors.
Transcription studies with highly purified potato cell nuclei in combination with a 'transcription-hybridization analysis' unequivocally demonstrate that the nucleus is the subcellular site where the entire process of PSTV replication takes place. Inhibition experiments with actinomycin D and alpha-amanitin furthermore suggest that the nuclear DNA-dependent RNA polymerases I and II are involved in the synthesis of PSTV (+) and (-) RNA, respectively.
D000546:Amanitins / Q000494:pharmacology; D002467:Cell Nucleus / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D010940:Plant Proteins / Q000378:metabolism; D010944:Plants / Q000378:metabolism / Q000382:microbiology; D012318:RNA Polymerase I / Q000037:antagonists & inhibitors; D012319:RNA Polymerase II / Q000037:antagonists & inhibitors; D012367:RNA, Viral / Q000096:biosynthesis*; D014158:Transcription, Genetic / Q000187:drug effects; D014772:Viroids / Q000235:genetics*; D014779:Virus Replication / Q000187:drug effects
D000546:Amanitins; D010940:Plant Proteins; D012367:RNA, Viral; D003609:Dactinomycin; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases; D012318:RNA Polymerase I