title: Synthesis and characterization of a radioiodinated, photoreactive and physiologically active analogue of platelet activating factor.
abstract: The multistep synthesis of a photoreactive, radioactive and aggregating analogue of platelet-activating factor (PAF)-acether is described. The photoreactive and radioactive moiety was added at the last step; the specific radioactivity was higher than 1000 Ci/mmol. The concentration of this new analogue which causes 50% of aggregation of platelets were of the same order of magnitude as for synthetic snPAF-acether, so as for two other analogues having a bulky group at the omega end of the fatty ether chain. The photoreactivity was proved by the covalent binding of the analogue to protein (BSA) after 10-min irradiation times at 300 nm. The binding was largely prevented by prior (not by later) addition of a high concentration of lyso phosphatidyl choline.
mesh_terms: D000107:Acetylation; D055598:Chemical Phenomena; D002621:Chemistry; D006801:Humans; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D009682:Magnetic Resonance Spectroscopy; D010972:Platelet Activating Factor / Q000032:analysis*
chemical_list: D007457:Iodine Radioisotopes; D010972:Platelet Activating Factor