Syrian hamster lymphocyte cytogenetic technique and effects of blood storage on mitotic index.
The Syrian hamster has a diploid chromosome complement similar to humans in both number (2N = 44) and morphology. For comparative mutagenic studies with humans, a repeatable lymphocyte chromosome technique involving laboratory mammals is desirable. The reported hamster lymphocyte cytogenetic technique appears technically uncomplicated and repeatable while providing a sufficient number of metaphase cells for quantitative analysis. In order to determine the effects of storing whole-blood (in culture media) for varying time periods at 8 degrees C prior to adding a mitogen, the mitotic index was calculated following a 48-h culture period. Results indicated that storage up to six days can still result in a mitotic index adequate for cytogenetic analyses.
D000818:Animals; D001793:Blood Preservation; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D006224:Cricetinae; D003470:Culture Media; D003582:Cytogenetics; D008214:Lymphocytes / Q000187:drug effects / Q000254:growth & development*; D008647:Mesocricetus; D008938:Mitosis / Q000187:drug effects; D008940:Mitotic Index / Q000187:drug effects; D010835:Phytohemagglutinins / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology
D003470:Culture Media; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D003208:Concanavalin A