Opsonic requirements for phagocytosis of Legionella micdadei by polymorphonuclear leukocytes.
The roles of the classical and alternative pathways of complement activation and of antibody in the phagocytosis of Legionella micdadei by polymorphonuclear leukocytes were studied. Normal serum was treated with the appropriate chelators or with heat to inactivate the classical, alternate, or both pathways of complement activation. Normal and complement-depleted sera with or without antibody were employed as opsonins for L. micdadei in phagocytosis assays. There was no difference in the phagocytosis of L. micdadei promoted by normal serum and either C4-deficient serum or serum in which the classical pathway had been inactivated. Both normal and classical pathway-deficient sera promoted significantly greater phagocytosis than did sera in which the alternate pathway or both the alternate and classical pathways had been inactivated. Thus, polymorphonuclear leukocyte phagocytosis of L. micdadei in the absence of antibody required an intact alternate pathway. Specific antibody partially restored opsonization to sera deficient in the alternate or both complement pathways, but phagocytosis was still significantly less than that with the alternate pathway intact.
D000906:Antibodies / Q000276:immunology; D003170:Complement Pathway, Alternative; D003165:Complement System Proteins / Q000276:immunology; D006801:Humans; D007875:Legionella / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D010587:Phagocytosis
D000906:Antibodies; D003165:Complement System Proteins