Aging in vivo and neuraminidase treatment of rat erythrocytes and their sequestration by the isolated perfused rat liver.
The isolated perfused rat liver as a model for phagocytosis leaves the specific arrangement of macrophages in the original tissue structure intact. Therefore phagocytosis may occur under terms closely approaching physiological conditions. Separation of rat erythrocytes according to their density in 13% young, 75% mature and 12% old cells permits the establishment of differences in the mean cellular volume (MCV), mean cellular hemoglobin concentration (MCHC) and content of N-acetylneuraminic acid per red blood cell (NANA/RBC). Perfusion of these fractions does not result in significant differences of sequestration of the isolated liver. Enzymatic release of 90% of membrane-bound sialic acid causes a significant increase of 20% in the elimination of erythrocytes from the perfusate. Treatment with neuraminidase is a trigger for sequestration either by adherence or by complete phagocytosis. Incubation of neuraminidase-treated erythrocytes with plasma does not increase the removal rate of cells during perfusion.
D000818:Animals; D004905:Erythrocyte Aging / Q000187:drug effects*; D004909:Erythrocyte Indices; D006452:Hemoglobinometry; D007728:Kupffer Cells / Q000502:physiology*; D008297:Male; D009439:Neuraminidase / Q000494:pharmacology*; D010477:Perfusion; D010587:Phagocytosis / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D012794:Sialic Acids / Q000378:metabolism
D012794:Sialic Acids; D009439:Neuraminidase