Analysis of different forms of recombinant human leukocyte interferons and synthetic fragments by high-performance liquid chromatography.
Analytical high-performance liquid chromatography (HPLC) conditions are reported for the evaluation of recombinant human interferon monomers: recombinant human leukocyte interferon A (rIFN-alpha A), rIFN-alpha D, and the hybrid rIFN-alpha A1-62/D64-166. The two monomeric forms of rIFN-alpha A (slow-migrating monomer and fast-migrating monomer) were also resolved by HPLC. Conditions are reported for the HPLC separation of oligomers (dimers and trimers) of rIFN-alpha A. The synthesis and analytical HPLC of the carboxy-terminus fragment, corresponding to IFN-alpha D (140-166), and a series of analogues comprising the IFN-alpha A (105-125) region is reported. The syntheses were accomplished by the solid-phase peptide synthesis procedure and the products were purified by preparative HPLC.
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D004274:DNA, Recombinant; D006801:Humans; D007370:Interferon Type I / Q000032:analysis* / Q000302:isolation & purification; D010446:Peptide Fragments / Q000302:isolation & purification
D004274:DNA, Recombinant; D007370:Interferon Type I; D010446:Peptide Fragments