1H-NMR spectra of rat synaptic plasma membranes: effect of temperature and comparison with fluorescence polarization.
1H-NMR spectra of rat synaptic plasma membranes obtained over the temperature range of 24-46 degrees C are presented. The data illustrate that a transition occurs from a more ordered to less ordered state at approximately 37 degrees C. This phenomenon was not related to using D2O as the solvent and was replicated, although with less sensitivity, using fluorescence polarization methodology.
D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D001921:Brain / Q000502:physiology*; D005454:Fluorescence Polarization; D005583:Fourier Analysis; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D051381:Rats; D013570:Synaptic Membranes / Q000502:physiology*; D013696:Temperature