A further assessment of the sub-nucleosomal product, "peak A", from Physarum polycephalum.
Nuclease digestion studies of Physarum polycephalum nuclei (1-3) and nucleoli (4) over the past few years have been centred on a number of modified nucleosomal products which have been related to active-gene regions of the genome. We have re-investigated one such particle, peak A, using the techniques of differential melting and polyacrylamide gel electrophoresis and show that this material is unlikely to be a specific histone:DNA complex as suggested by earlier authors.
D000818:Animals; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000032:analysis* / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D002645:Chickens; D004247:DNA / Q000032:analysis / Q000378:metabolism; D004355:Drug Stability; D004912:Erythrocytes / Q000648:ultrastructure; D006657:Histones / Q000032:analysis / Q000378:metabolism; D006358:Hot Temperature; D046911:Macromolecular Substances; D008836:Micrococcal Nuclease / Q000378:metabolism; D010804:Physarum / Q000648:ultrastructure*
D006657:Histones; D046911:Macromolecular Substances; D004247:DNA; D008836:Micrococcal Nuclease