Mass characteristics of DNA obtained from chromosomes of a human carcinoma cell line.
Sedimentation studies of DNA from chromosomes extracted from human mitotic cells showed that high-molecular-weight DNA can be obtained if cell hypotonic treatments and prolonged metaphase blocks are avoided. Two types of large double-stranded DNA were observed. One of these (Mr = 2.5 X 10(8)) appeared as a size class with characteristics reminiscent of the chromosomal DNA subunit hypothesis. However, this DNA is the decay product of larger molecules, whose minimum molecular weight is 6 X 10(8).
D000970:Antineoplastic Agents / Q000494:pharmacology; D001562:Benzimidazoles / Q000494:pharmacology; D002458:Cell Fractionation; D002460:Cell Line; D002877:Chromosomes, Human / Q000648:ultrastructure; D004261:DNA Replication; D004273:DNA, Neoplasm / Q000302:isolation & purification*; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D008677:Metaphase / Q000187:drug effects; D008938:Mitosis / Q000187:drug effects; D008970:Molecular Weight; D015739:Nocodazole; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D000970:Antineoplastic Agents; D001562:Benzimidazoles; D004273:DNA, Neoplasm; D014316:Tritium; D015739:Nocodazole; D013936:Thymidine; D006918:Hydroxyurea