title: Characterization of a 22 kDa protein with widespread tissue distribution but which is uniquely present in secretions of the testis and epididymis and on the surface of spermatozoa.
abstract: The purification is reported of a 22 kDa protein which was first identified as one of the major components of the luminal secretion of the rat testis and epididymis. Antibodies against the 22 kDa protein cross-reacted with a protein of the same molecular weight in cytosolic extracts of other tissues from both male and female rats. However, since the protein could not be detected in blood, peritoneal fluid, saliva, milk, uterine fluid, seminal vesicle secretion, coagulating gland secretion or prostatic secretion, it would appear that the testis and epididymis may be unique in containing the protein in a soluble form within their luminal secretions. Proteins with slightly lower molecular weight were detected by the antibodies in cytosolic extracts of tissues from other animals (mice, rabbits, sheep, pigs, cattle), indicating that the protein may be conserved in a variety of species. However, in contrast to the rat, the protein was apparently not present in the testicular and epididymal secretions of these species. In addition to the occurrence of the 22 kDa protein as a soluble moiety in rat testicular and epididymal fluids, the protein was also located on sperm plasma membranes where its distribution was restricted to the surface of the flagellum. Amongst sperm surface proteins, the 22 kDa protein was the major protein containing sulphydryl groups and one of the major entities containing disulphide bonds. These properties may be of importance in the maintenance of sperm viability.
mesh_terms: D000818:Animals; D001202:Ascitic Fluid; D002417:Cattle; D003429:Cross Reactions; D004822:Epididymis / Q000378:metabolism*; D005122:Exudates and Transudates / Q000032:analysis*; D005260:Female; D008297:Male; D051379:Mice; D008856:Microscopy, Fluorescence; D008970:Molecular Weight; D011506:Proteins / Q000302:isolation & purification*; D011817:Rabbits; D051381:Rats; D012463:Saliva / Q000032:analysis; D012756:Sheep; D013045:Species Specificity; D013094:Spermatozoa / Q000032:analysis*; D013499:Surface Properties; D013552:Swine; D013737:Testis / Q000378:metabolism*; D014018:Tissue Distribution; D014599:Uterus / Q000378:metabolism
chemical_list: D011506:Proteins