title: Changes in protein phosphorylation during the cell cycle of Chinese hamster ovary cells.
abstract: The phosphorylation patterns of proteins were examined during the cell cycle of Chinese hamster ovary cells. This was accomplished by labeling synchronized cells at various times with [32P]orthophosphate and separating the proteins by both isoelectric focusing and nonequilibrium pH gradient two-dimensional gel electrophoresis. The most dramatic changes occurred during late G2/M when approximately eight proteins (including vimentin, lamin B, and histones 1 and 3) showed increased phosphorylation. Ten other proteins appeared to be uniquely phosphorylated during late G2/M. Of these 10 proteins, seven were no longer phosphorylated shortly after mitosis. There is also at least one protein which showed a relative decrease in phosphorylation during late G2/M.
mesh_terms: D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D006657:Histones / Q000378:metabolism; D007399:Interphase; D007700:Kinetics; D008970:Molecular Weight; D010053:Ovary; D010710:Phosphates / Q000378:metabolism; D010750:Phosphoproteins / Q000302:isolation & purification*; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D011506:Proteins / Q000378:metabolism*
chemical_list: D006657:Histones; D010710:Phosphates; D010750:Phosphoproteins; D010761:Phosphorus Radioisotopes; D011506:Proteins