Determination of reducing sugars in the nanomole range with tetrazolium blue.
Addition of sodium potassium tartrate to basic solutions of tetrazolium blue [(2,2',5,5'-tetraphenyl-3,3'-dimethoxy 4,4'-biphenylene) ditetrazolium chloride] greatly improved their efficacy as colorimetric reagents for reducing sugar determination. This modification increased sensitivity and decreased reaction time. The modified reagent can determine as little as 1 nmol of neutral sugars as well as 2-amino and N-acetyl amino sugars.
D002241:Carbohydrates / Q000032:analysis*; D003124:Colorimetry; D005947:Glucose / Q000032:analysis; D007700:Kinetics; D008832:Microchemistry / Q000379:methods; D009580:Nitroblue Tetrazolium; D013644:Tartrates; D013696:Temperature; D013778:Tetrazolium Salts
D002241:Carbohydrates; D013644:Tartrates; D013778:Tetrazolium Salts; D009580:Nitroblue Tetrazolium; D005947:Glucose; C029768:tartaric acid