Parvovirus serologically related to the minute virus of mice (MVM) as contaminant of BHK 21 cl. 13 suspension cells.
From BHK 21 cl. 13 suspensions cells we have isolated a virus serologically related to the Minute Virus of Mice (MVM); the same virus has been isolated from the same cells in U.K. in 1980. Besides isolating this virus from large scale cultures, we have also isolated it from many of the sublines stored in nitrogen we received at different times from different laboratories. At this time it is difficult to ascertain if the contamination originated from the serum or from other components of the medium; furthermore we do not know if many of the cells of our stock became contaminated in our laboratory or if they were already contaminated.
D000818:Animals; D002999:Clone Cells / Q000382:microbiology*; D006224:Cricetinae; D003588:Cytopathogenic Effect, Viral; D007668:Kidney; D008915:Minute Virus of Mice / Q000276:immunology / Q000302:isolation & purification*; D010321:Parvoviridae / Q000276:immunology / Q000302:isolation & purification*