[Mechanisms of radiation impairment of DNA biosynthesis. Separation, partial purification, characteristics of and changes in DNA polymerase alpha and beta activities of rat bone marrow at early stages after gamma irradiation and comparison with the status of repair and replication biosynthesis of DNA].
Activity of alpha and beta DNA-polymerases in nuclei and cytosol and also replicative and repair biosynthesis of DNA in the bone marrow were determined during the first hours following whole-body gamma-irradiation of rats with a dose of 6 Gy. There was a pronounced and reliable correlation between postirradiation changes in activities of both DNA-polymerases in nuclei and also semiconservative and repair biosynthesis of DNA in bone marrow cells. The results were obtained by the method of separation and partial purification of alpha and beta polymerase the most important characteristics of which are presented.
D000818:Animals; D001853:Bone Marrow / Q000201:enzymology / Q000528:radiation effects*; D003037:Cobalt Radioisotopes; D004247:DNA / Q000528:radiation effects*; D004256:DNA Polymerase I / Q000302:isolation & purification / Q000378:metabolism*; D004257:DNA Polymerase II / Q000302:isolation & purification / Q000378:metabolism*; D004260:DNA Repair / Q000528:radiation effects*; D004261:DNA Replication / Q000528:radiation effects*; D005720:Gamma Rays; D051381:Rats; D011919:Rats, Inbred Strains
D003037:Cobalt Radioisotopes; D004247:DNA; D004256:DNA Polymerase I; D004257:DNA Polymerase II
Mekhanizmy radiatsionnogo narusheniia biosinteza DNK. Razdelenie, chastichnaia ochistka, kharakteristika i izmenenie aktivnosti DNK-polimeraz al'fa i beta kostnogo mozga u krys v rannie sroki posle gamma-oblucheniia, sopostavlennie s sostoianiem reparatsionnogo i replikatsionnogo biosinteza DNK.