title: Analysis of human spermatozoa before and after processing for in vitro fertilization.
abstract: Washed incubated spermatozoa processed for in vitro fertilization were compared to the spermatozoa from the same unprocessed ejaculate in 31 randomly selected normal males (Group 1) and 7 subfertile males (Group 2). In Group 1 there was a significant increase in motility (P = 0.001) as well as a significant increase in normal morphology (P = 0.001). All categories of morphologically abnormal sperm decreased, with a significant decrease in those exhibiting small heads (P = 0.01), tapered heads (P = 0.01), cytoplasmic droplets (P = 0.001), bent midpieces (P = 0.01), or coiled tails (P = 0.02). There was also a significant decrease in immature germ cells (P = 0.001) as well as white blood cells (P = 0.02). Group 2 also showed a significant increase in motility (P = 0.01) and a significant increase in normal morphology (P = 0.01). There was a decrease in various categories of abnormal sperm, with a significant decrease in sperm with cytoplasmic droplets (P = 0.02).
mesh_terms: D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology*