Incorporation of 3H-labeled nucleosides and 3H-labeled deoxynucleosides into detergent soluble DNA.
Detergent soluble DNA from splenocytes of immunologically stimulated mice has been shown to incorporate [3H]dThd more rapidly than detergent insoluble DNA. In this report we compare the incorporation of other 3H-labeled nucleosides and 3H-labeled deoxynucleosides and the distribution of 3H in the different size classes of detergent soluble DNA. The order of incorporation into DS DNA is [3H]dThd greater than [3H]dCyd greater than [3H]Ado greater than [3H]dGuo approximately equal to [3H]Cyd greater than [3H]dAdo greater than [3H]Guo. We also show that the previously reported slight enrichment in Gua + Cyt content is not due to preferential incorporation of dGuo or of dCyd into any one size class.
D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D003853:Deoxyribonucleosides / Q000378:metabolism*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D051379:Mice; D008970:Molecular Weight; D009705:Nucleosides / Q000378:metabolism*; D017830:Octoxynol; D011092:Polyethylene Glycols; D012995:Solubility; D013154:Spleen
D003853:Deoxyribonucleosides; D009705:Nucleosides; D011092:Polyethylene Glycols; D017830:Octoxynol; D004247:DNA; C010615:Nonidet P-40