Differences in the response of non-dividing human diploid fibroblasts (HDF) to inducers of DNA synthesis generated by cycling HDF and HeLa cells.
The ability of non-dividing WI-38 cells from different passages to respond to inducers of DNA synthesis generated by other WI-38 cells in G1 or S and HeLa cells in S was studied. The non-dividing cells are refractory to inducers of DNA synthesis generated by WI-38 cells in S but respond to HeLa inducers after a lag period of 3 hours. The WI-38 cells in G1 are not inhibited from entering S in the presence of non-dividing cell nuclei in the heterokaryons.
D002455:Cell Division; D002459:Cell Fusion; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D005347:Fibroblasts / Q000378:metabolism*; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007399:Interphase
D004247:DNA