Biochemical characterization of five glycoprotein molecules of the porcine zona pellucida.
Five glycoproteinmolecules with the molecular masses of 17 000; 38 000; 42 000; 50 000 and 67 000 were purified by high performance liquid chromatography following solubilization of isolated porcine zonae pellucidae by treatment with lithium-3,5-diiodosalicylate. The N-terminal amino acid residues were identified as arginine for 67 000, alanine for 50 000, arginine for 42 000, alanine for 38 000 and histidine for 17 000. The glycopeptides 42 000 and 17 000 were found to be rich on carbohydrates and 67 000 contained 7, 16% sialic acids. The latter moieties were tentatively identified as 5-N-acetylneuraminic acid, 5-N-glycolylneuraminic acid and 5-N-acetyl-7,8,9 tri-O-acetylneuraminic acid. The five components of the zona were resolved by thin layer chromatography in a solvent system of propanol/butanol/HCl (2:1:1) and showed Rf-values of 0.17, 0.42, 0.46, 0.50 and 0.55 respectively. The glycoprotein with the molecular mass of 38 000 possesses spermatozoal receptor properties. This receptor molecule showed a pI of 5.9 upon isoelectric focusing.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001345:Autoradiography; D002241:Carbohydrates / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004527:Egg Proteins / Q000032:analysis*; D005260:Female; D006023:Glycoproteins / Q000032:analysis*; D007463:Iodobenzoates; D007525:Isoelectric Focusing; D008970:Molecular Weight; D010063:Ovum / Q000032:analysis*; D012459:Salicylates; D012995:Solubility; D013552:Swine; D015044:Zona Pellucida / Q000032:analysis*
D000596:Amino Acids; D002241:Carbohydrates; D004527:Egg Proteins; D006023:Glycoproteins; D007463:Iodobenzoates; D012459:Salicylates; C020891:3,5-diiodosalicylic acid