title: Morphologic and functional evidence for oxygen deficiency in the isolated perfused rat kidney.
abstract: Morphologic and functional studies were undertaken in the isolated rat kidney, perfused with an albumin-Krebs-Henseleit solution, to which 5% human erythrocytes and/or various amino acids had been added. Perfused only with the albumin-Krebs-Henseleit solution, the kidneys displayed a characteristic pattern of cell necrosis after 2 hours of perfusion, which was confined to the interbundle region of the outer medulla and was not evident in either the cortex or the inner medulla. In the outer stripe only those proximal straight tubules (P3 segments) farthest from the vascular bundles were damaged. In the inner stripe only those thick ascending loops of Henle at the periphery of the vascular bundles escaped damage; all thick ascending loops of Henle lying farthest from the bundles were severely damaged. The number of damaged tubules increased toward the border to the inner medulla. Necroses in both segments, P3 and thick ascending loops of Henle, could be prevented by perfusion with the erythrocyte-albumin-Krebs-Henseleit solution but not by the addition of glutathione, in the absence of erythrocytes. Perfusion with the erythrocyte medium also significantly improved glomerular filtration rate and sodium and glucose reabsorption. It is concluded that, in the isolated, erythrocyte-free perfused kidney, the oxygen content of the "arterial" vasa recta in the vascular bundles is only sufficient to supply the tubules in the immediate surroundings. Countercurrent exchange in the vascular bundles between arterial and "venous" vasa recta progressively lowers the arterial oxygen content as the inner stripe of the outer medulla is approached and with it the number of tubules receiving an adequate oxygen supply.
mesh_terms: D000818:Animals; D005919:Glomerular Filtration Rate; D005978:Glutathione / Q000172:deficiency; D000860:Hypoxia / Q000473:pathology* / Q000503:physiopathology; D066298:In Vitro Techniques; D007668:Kidney / Q000098:blood supply / Q000473:pathology* / Q000503:physiopathology; D007679:Kidney Medulla / Q000473:pathology; D007687:Kidney Tubules, Proximal / Q000473:pathology; D008138:Loop of Henle / Q000473:pathology; D008297:Male; D009336:Necrosis; D010101:Oxygen Consumption; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D005978:Glutathione