Enhancement by estrogen treatment of -bungarotoxin binding in fetal mouse amygdala cells reaggregated in vitro.
Dissociated amygdala cells from 17-day-old mouse fetuses were cultivated in a flask for 7 days to form aggregates. In the aggregates there developed typical synaptic structures and [125I]alpha-bungarotoxin binding capacity. An addition of estradiol (100 ng/ml) in the culture medium caused a significant increase in the toxin binding capacity. From these results it is concluded that estradiol enhances alpha-bungarotoxin binding capacity in the amygdala by exerting direct effects on the developing amygdala cells.
D000679:Amygdala / Q000378:metabolism* / Q000648:ultrastructure; D000818:Animals; D002038:Bungarotoxins / Q000378:metabolism*; D002449:Cell Aggregation; D002478:Cells, Cultured; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D012737:Sex Factors
D002038:Bungarotoxins; D004958:Estradiol