Analysis of the calcium-dependent interaction of calmodulin with bovine serum albumin.
An air-driven ultracentrifuge has been used to investigate the calcium-dependent association between calmodulin and bovine serum albumin. Procedures were described which allowed the interaction to be analyzed to yield the equilibrium constant. At low ionic strength (25 mM Tris-HCl, pH 7.5, pCa 6.68, 9 degrees C) the equilibrium constant for the interaction was estimated to be 2.1 X 10(4) M-1, while at high ionic strength (25 mM Tris-HCl, pH 7.5, 150 mM KCl, pCa 6.68, 9 degrees C) the value was 4.5 X 10(3) M-1. Under similar conditions, calmodulin was also found to interact with beta-lactoglobulin A and gelatin, but no detectable association was observed with ovalbumin.
D000818:Animals; D002118:Calcium / Q000502:physiology*; D002147:Calmodulin / Q000097:blood*; D002417:Cattle; D005476:Fluphenazine / Q000494:pharmacology; D008433:Mathematics; D008956:Models, Chemical; D011485:Protein Binding / Q000187:drug effects; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013997:Time Factors; D014461:Ultracentrifugation
D002147:Calmodulin; D012710:Serum Albumin, Bovine; D005476:Fluphenazine; D002118:Calcium