title: N.m.r. studies of the disulphated disaccharide obtained by degradation of bovine lung heparin with nitrous acid.
abstract: The disulphated disaccharide IdoA(2SO3)-anManOH(6SO3) was prepared from bovine lung heparin by treatment with nitrous acid followed by borohydride reduction. The 1H- (400 MHz) and 13C-n.m.r. (100 MHz) spectra of this disaccharide derivative have been assigned completely using homonuclear spin-decoupling experiments, 13C-1H correlations, and a COSY-45 two-dimensional homonuclear correlation experiment. The 3JH,H values show that the IdoA(2SO3) residue exists in a single conformation throughout the temperature range 20-90 degrees.
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002417:Cattle; D004187:Disaccharides / Q000032:analysis*; D006493:Heparin; D008168:Lung / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013464:Sulfuric Acids / Q000032:analysis
chemical_list: D004187:Disaccharides; D013464:Sulfuric Acids; D006493:Heparin