title: Genetics, subcellular localization, and molecular characterization of 6-phosphogluconate dehydrogenase isozymes in tomato.
abstract: Three independent genes are responsible for 6-phosphogluconate dehydrogenase (6PGDH) activity in tomato. 6Pgdh-2, located on chromosome 12, codes for subunits of an active dimer which is restricted to the plastids. 6Pgdh-1, chromosome 4, and 6Pgdh-3, chromosome 5, code for subunits which form three dimers--two homodimers and an intergenic heterodimer. The latter three isozymes are found in the cytosol. 6Pgdh-1, 6Pgdh-2, and 6Pgdh-3 code for subunits with estimated molecular weights of 49,500, 50,500, and 51,200, respectively. The intergenic heterodimer encoded by 6Pgdh-1 and 6Pgdh-3 is thus composed of subunits that differ in length by approximately 15 amino acid residues. Divergence in the length and primary subunit structure may account for the lower thermal stability of the intergenic heterodimer compared with the corresponding homodimers. A limited survey of other solanaceous plant species suggests that the duplication of cytosolic 6PGDH-coding genes found in tomato may be widespread in the family.
mesh_terms: D000483:Alleles; D002736:Chloroplasts / Q000201:enzymology; D002871:Chromosome Banding; D003433:Crosses, Genetic; D007527:Isoenzymes / Q000235:genetics* / Q000302:isolation & purification; D007700:Kinetics; D046911:Macromolecular Substances; D008970:Molecular Weight; D010734:Phosphogluconate Dehydrogenase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D010944:Plants / Q000201:enzymology*; D013347:Subcellular Fractions / Q000201:enzymology
chemical_list: D007527:Isoenzymes; D046911:Macromolecular Substances; D010734:Phosphogluconate Dehydrogenase