Quality control study for estradiol receptor determination.
Lyophilized calf uterine cytosol standards for estradiol receptor (ER) were prepared separately in two institutes. The ER binding capacity was determined by dextran-coated charcoaL (DCC)-technique using a multi-point Scatchard analysis. The intra- and inter-assay coefficient of variation determined on the Hungarian preparation were 13.10% and 14.70%. The dissociation constant (Kd) values of all the samples ranged between 1.2 X 10(-9) M and 9.35 X 10(-10) M. Standards stored at--70 degrees C and at 0-4 degrees C proved to be stable for several months. At ambient temperature, a progressive decline of the ER activity was observed during a 1 year storage period. The use of the lyophilized calf uterine cytosol standards stored at 0-4 degrees C is recommended for both intra- and inter-laboratory quality control study in multicentric clinical trials of breast cancer.
D001943:Breast Neoplasms / Q000032:analysis; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007753:Laboratories / Q000592:standards; D011786:Quality Control; D011959:Receptors, Estradiol / Q000032:analysis*; D011960:Receptors, Estrogen / Q000032:analysis*
D011959:Receptors, Estradiol; D011960:Receptors, Estrogen