Continuous body fluid monitoring for zomepirac by fully automated high-performance liquid chromatography.
A sensitive, specific and rapid determination of 5-(4-chlorobenzoyl)-1,4-dimethyl-1H-pyrrole-2-acetic acid sodium salt dihydrate (zomepirac sodium, Zomax) in human plasma and urine by a continuous body fluid monitoring system based on high-performance liquid chromatography is described. Samples are directly injected into the apparatus which consists of commonly available HPLC-modules. Manual sample clean-up procedures as well as the addition of an internal standard are not needed. Using 50-microliter aliquots, the detection limit is lower than 0.05 microgram/ml and the calibration ranges from 0.5 to 100 micrograms/ml are linear for both, spiked samples and references. The recovery is 97% for plasma and 95% for urine samples at a sampling rate of about 15 samples/h.
D000700:Analgesics / Q000032:analysis* / Q000097:blood / Q000652:urine; D001826:Body Fluids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D006801:Humans; D011758:Pyrroles / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D014046:Tolmetin / Q000031:analogs & derivatives / Q000032:analysis* / Q000097:blood / Q000652:urine
D000700:Analgesics; D011758:Pyrroles; C020549:zomepirac; D014046:Tolmetin