Interaction between yeast cytochrome P-450 and chemical carcinogens.
The binding of various chemical carcinogens to the glucose-inducible form of cytochrome (cyt.) P-450 in Saccharomyces cerevisiae was examined with reference to known metabolism in mammalian systems. All the carcinogens examined gave spectral interactions with the yeast cytochrome. The carcinogens benzo[a]pyrene and cyclophosphamide gave Type I binding, while benzidine and 2-naphthylamine gave Type II binding spectra indicating binding at the haem group. Dimethylnitrosamine gave undetectable binding in yeast microsomal fractions, but a Type I binding spectrum was seen with purified cyt. P-450 preparations. The demonstration that these carcinogens bind to yeast cyt. P-450 is discussed in the light of their genotoxicity.
D015081:2-Naphthylamine / Q000378:metabolism; D001560:Benzidines / Q000378:metabolism; D001564:Benzo(a)pyrene / Q000378:metabolism; D002273:Carcinogens / Q000378:metabolism*; D003520:Cyclophosphamide / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D007700:Kinetics; D008861:Microsomes / Q000378:metabolism; D015003:Yeasts / Q000378:metabolism*
D001560:Benzidines; D002273:Carcinogens; C029876:benzidine; D001564:Benzo(a)pyrene; D003520:Cyclophosphamide; D003577:Cytochrome P-450 Enzyme System; D015081:2-Naphthylamine