title: Phorbol ester and sperm activate mouse oocytes by inducing sustained oscillations in cell Ca2+.
abstract: The parthenogenetic activation of mouse oocytes by several agents is accompanied by a large rise in the intracellular calcium concentration ( [Ca2+]i). The tumour-promoting phorbol ester 12-O-tetradecanoyl phorbol acetate (TPA) activates some cellular processes which are also activated by raised [Ca2+] (ref. 2). We therefore tested TPA on mouse oocytes and found it to be a potent parthenogenetic activating agent. Recent reports suggest that TPA mimics endogenous diacylglycerol3 and can stimulate cells by activating protein kinase C without raising [Ca2+]i (refs 4, 5). We have now measured [Ca2+]i with aequorin in mouse oocytes treated with TPA. Sustained oscillations in [Ca2+]i appeared in those oocytes which were subsequently activated. We also measured [Ca2+]i during fertilization. The response began with [Ca2+]i transients as reported previously, but the transients continued in a regular pattern for 4 h. There was no rise in [Ca2+]i of the type induced by activating agents such as ethanol. One component of the fertilization response appears to be related to the TPA-induced oscillations.
mesh_terms: D000818:Animals; D002118:Calcium / Q000502:physiology*; D005260:Female; D005306:Fertilization; D008297:Male; D051379:Mice; D009865:Oocytes / Q000502:physiology*; D010507:Periodicity; D010703:Phorbol Esters / Q000494:pharmacology*; D010704:Phorbols / Q000494:pharmacology*; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000502:physiology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D010703:Phorbol Esters; D010704:Phorbols; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium