Analysis of soybean sapogenins by high-performance liquid chromatography.
A method for the analysis of soybean sapogenins is described. The method is based on the extraction of soybean saponins from a defatted sample. The triterpene glycosides are then hydrolysed with subsequent analysis of the liberated sapogenins by high-performance liquid chromatography using gradient elution and mass detection. By use of a sapogenin/carbohydrate ratio, an estimate of the total saponin content can be made.
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005433:Flour / Q000032:analysis; D006868:Hydrolysis; D008027:Light; D012502:Sapogenins / Q000032:analysis*; D012542:Scattering, Radiation; D013025:Glycine max / Q000032:analysis*
D012502:Sapogenins