Determination of oestriol in pregnancy urine by normal-phase high-performance liquid chromatography with electrochemical detection.
A method for the determination of oestriol in pregnancy urine by normal-phase high-performance liquid chromatography with electrochemical detection is described. A large-volume wall-jet cell with an Ag-Ag+ reference electrode was used as the detector system. The limit of detection obtained is comparable to that of electrochemical detection following reversed-phase liquid chromatography. One of the advantages of electrochemical detection with normal-phase systems is that adsorption problems are minimized.
D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry; D004566:Electrodes; D004958:Estradiol / Q000652:urine; D004964:Estriol / Q000652:urine*; D004970:Estrone / Q000652:urine; D005260:Female; D006801:Humans; D006868:Hydrolysis; D008297:Male; D011247:Pregnancy; D013050:Spectrometry, Fluorescence
D004970:Estrone; D004958:Estradiol; D004964:Estriol