Brain cerebrosides: preparative purification using a new chromatographic support--magnammsil.
A preparative method for isolation and purification of cerebrosides from bovine brain is described. A crude cerebroside fraction is precipitated with ether from a chloroform-methanol extract and purification is achieved by column chromatography on a new column support, "magnammsil." A partial separation of hydroxy and nonhydroxy cerebrosides is achieved. The method is more economical for a large-scale preparation than previously published methods.
D019342:Acetic Acid; D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002554:Cerebrosides / Q000302:isolation & purification*; D002845:Chromatography; D008274:Magnesium; D012824:Silicic Acid
D002554:Cerebrosides; D012824:Silicic Acid; D008274:Magnesium; D019342:Acetic Acid