Isolation and characterization of nerve growth factor from Vipera lebetina (snake) venom.
Nerve growth factor from Vipera lebetina venom was purified by gel filtration and ion exchange chromatography steps. The NGF preparation obtained is a glycoprotein with weak arginine esterase activity. It hydrolyzes benzoylarginine ethyl ester (BAEE). Vipera lebetina NGF consists of multiple forms of protein with pI in the interval 9-10.5. All isoforms have identical molecular weights of 32,500.
D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D002642:Chick Embryo; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005724:Ganglia / Q000187:drug effects; D066298:In Vitro Techniques; D007525:Isoelectric Focusing; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000302:isolation & purification* / Q000494:pharmacology; D012910:Snake Venoms / Q000302:isolation & purification; D012911:Snakes; D013045:Species Specificity; D014757:Viper Venoms / Q000302:isolation & purification*
D009414:Nerve Growth Factors; D012910:Snake Venoms; D014757:Viper Venoms; C019724:arginine esterase; D002265:Carboxylic Ester Hydrolases