Differentiation of chick blood island erythroblasts into erythrocytes and stability in long-term culture.
Clusters of 20-70 erythroblasts from blood islands of early chick blastoderm were cultured in serum-free chemically defined medium for a 3-month period. The erythroblast cluster produces erythroid cells and hemoglobins characteristic of the primitive and definitive erythroid cell lines. It seems there is a progenitor erythroid cell(s) in the erythroblast cluster which starts and/or continues maturing along various pathways of hemopoietic differentiation under simple culture conditions. The erythroid character of these cells is stable during the 3-month culture period.
D000818:Animals; D001756:Blastoderm / Q000502:physiology; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D002642:Chick Embryo; D004900:Erythroblasts / Q000166:cytology* / Q000502:physiology; D004920:Erythropoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000502:physiology; D006454:Hemoglobins / Q000096:biosynthesis
D006454:Hemoglobins