Enzymatic denitrification of 2-nitropropane in uninduced mouse liver microsomes.
Hepatic microsomes from 5 strains of untreated mice were tested for the ability to enzymatically cleave the nitro group from 2-nitropropane (2NP). All strains showed significant NADPH-dependent nitrite release at pH 7.6 and pH 8.8. Statistical differences in nitrite-releasing activity between strains were found between BALB and PL/J and ATH strains at pH 7.6. At pH 8.8, BIO.M differed from CD-1 and BALB. These results are in contrast to a report of little or no denitrification activity in uninduced rats and suggest that the 2NP microsomal metabolism may be of greater importance than previously thought.
D000473:Alkanes / Q000378:metabolism*; D000818:Animals; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism*; D009595:Nitroparaffins / Q000378:metabolism*; D011407:Propane / Q000031:analogs & derivatives* / Q000378:metabolism; D013045:Species Specificity
D000473:Alkanes; D009573:Nitrites; D009595:Nitroparaffins; C016617:2-nitropropane; D011407:Propane