An early effect of the S component of staphylococcal leukocidin on methylation of phospholipid in various leukocytes.
On incubation of rabbit polymorphonuclear leukocytes with the S component of staphylococcal leukocidin at 37 degrees C, the 3H-labeled methyl group of S-adenosyl[methyl-3H]methionine was rapidly incorporated into phospholipid. Subsequently, the methylated phosphatidylcholine was degraded by activated phospholipase A2. Complete blockage of the methylation of phospholipid by a mixture of erythro-9-[2-hydroxy-3-nonyl]adenine, adenosine, and L-homocysteine thiolactone markedly inhibited the activation of phospholipase A2 by the S component. It also inhibited the binding of 125I-labeled F component to the cells, but not that of the labeled S component. These results suggest that methylation of phospholipid in the cell membranes by the S component results in activation of phospholipase A2, which induces the binding of the F component to the cells.
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000241:Adenosine / Q000494:pharmacology; D000818:Animals; D002470:Cell Survival; D004789:Enzyme Activation; D006710:Homocysteine / Q000494:pharmacology; D007956:Leukocidins / Q000494:pharmacology*; D008563:Membrane Lipids / Q000378:metabolism*; D008745:Methylation; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D010743:Phospholipids / Q000378:metabolism*; D011817:Rabbits; D013210:Staphylococcus / Q000472:pathogenicity*
D007956:Leukocidins; D008563:Membrane Lipids; D010743:Phospholipids; D006710:Homocysteine; C010192:9-(2-hydroxy-3-nonyl)adenine; D010741:Phospholipases A; D054467:Phospholipases A2; D000225:Adenine; D000241:Adenosine