title: Ultrastructural morphometry of parathyroid cells in rats of different ages.
abstract: Parathyroid glands of newborn to 1-year-old male Sprague-Dawley rats were fixed by perfusion or by immersion, and prepared for electron microscopy. Parathyroid glands fixed by immersion exhibited parenchymal cells with variable ultrastructure, indicating that these cells were in different stages of the proposed secretory cycle in parathyroid cells. In contrast, parathyroid cells of glands fixed by perfusion were uniform in ultrastructure, suggesting that all cells were in the same stage of secretory activity. Parathyroid glands of 3-, 4-, 6-, 8- and 10-week-old rats also were fixed by perfusion and analysed by electron-microscopic morphometry. These data demonstrated an increase in cell volume and in surface area of the membrane compartments concerned with parathyroid hormone secretion: these changes were not related to variations in serum calcium concentration. Both the qualitative observations and the quantitative data do not favour the idea of a secretory cycle in rat parathyroid cells.
mesh_terms: D000375:Aging; D000818:Animals; D000831:Animals, Newborn / Q000033:anatomy & histology; D002118:Calcium / Q000097:blood; D005404:Fixatives; D008297:Male; D008433:Mathematics; D008854:Microscopy, Electron; D010280:Parathyroid Glands / Q000502:physiology / Q000648:ultrastructure*; D010281:Parathyroid Hormone / Q000378:metabolism; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D005404:Fixatives; D010281:Parathyroid Hormone; D002118:Calcium