Crosslinking of chromosomal antigen common in human tumors to DNA by cis-diamminedichloroplatinum (II).
The antisera specific for dehistonized HeLa cell chromatin were obtained by injecting rabbits or goats. Treatment of chromatin with cis-DDP crosslinked the active proteins to DNA thus preventing dissociation of the proteins in a high salt environment. Immunochemical staining of electrophoretically separated chromosomal proteins transferred to nitrocellulose sheets revealed that cis-DDP among others crosslinked the protein with m.w. of about 81 000. This protein is the only major protein antigen presented in several human tumors and absent in normal human tissues.
D000951:Antigens, Neoplasm; D002843:Chromatin / Q000187:drug effects* / Q000276:immunology; D002945:Cisplatin / Q000494:pharmacology*; D003432:Cross-Linking Reagents; D004247:DNA / Q000378:metabolism*; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008970:Molecular Weight
D000951:Antigens, Neoplasm; D002843:Chromatin; D003432:Cross-Linking Reagents; D004247:DNA; D002945:Cisplatin