title: Schistosoma mansoni: lectin-dependent cytotoxicity of hemocytes from susceptible host snails, Biomphalaria glabrata.
abstract: Normally benign hemocytes from a strain (M-line) of the snail, Biomphalaria glabrata, susceptible to Schistosoma mansoni, became cytotoxic toward the sporocyst stage if the parasite was first treated with the lectin, concanavalin A. Concanavalin A binding was inhibitable with alpha-methyl mannoside and killing was dose-dependent. Maximal levels of concanavalin A-induced cytotoxicity were comparable with levels observed when hemocytes from a resistant snail strain (13-16-R1) encountered untreated sporocysts. Induction of the cytotoxic response did not occur if hemocytes alone were pretreated with the lectin. A unique method incorporating ultraviolet microscopy and the vital fluorescent dye, eosin Y, was used for discriminating between live and dead sporocysts. This model may prove useful in understanding mechanisms used by invertebrate effector cells in recognition and killing of invading organisms.
mesh_terms: D000818:Animals; D001700:Biomphalaria / Q000378:metabolism / Q000469:parasitology*; D001773:Blood Cells / Q000469:parasitology*; D003208:Concanavalin A / Q000378:metabolism*; D005690:Galactose; D006434:Hemocytes / Q000378:metabolism / Q000469:parasitology*; D006790:Host-Parasite Interactions; D008766:Methylmannosides; D012550:Schistosoma mansoni / Q000254:growth & development / Q000378:metabolism*
chemical_list: D008766:Methylmannosides; D003208:Concanavalin A; C008466:methylmannoside; D005690:Galactose