The effects of econazole on the growth of murine myeloma cells and its use in hybridoma production.
The effect of econazole, an antifungal agent, on the stability of NS1/Ag4 myeloma cells in culture was investigated with the view to its use in the control of fungal contamination in lymphocyte hybridoma monoclonal antibody production. The recommended dose of 1 microgram/ml was found to reduce cell numbers, viability and survival, and to effect dramatic dose related changes in DNA synthesis in the NS1 myeloma line and hence econazole is not suitable as an added antifungal agent in monoclonal antibody production using NS1 cells and their derived hybridomas.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004464:Econazole / Q000494:pharmacology*; D006825:Hybridomas / Q000187:drug effects* / Q000276:immunology; D007093:Imidazoles / Q000494:pharmacology*; D051379:Mice; D009101:Multiple Myeloma / Q000276:immunology / Q000378:metabolism / Q000473:pathology*
D000911:Antibodies, Monoclonal; D004273:DNA, Neoplasm; D007093:Imidazoles; D004464:Econazole