Precocious increase of sucrase activity by carbohydrates in the small intestine of suckling rats. II. Role of digestibility of sugars, osmolality, and stomach evacuation in producing diarrhea.
The mechanisms of carbohydrate-induced diarrhea in suckling rats were investigated with respect to osmolality and type of sugar in the milk. Groups of 12-day-old rats were gavage fed either a basic low carbohydrate milk formula [10.8% fat; 8% protein; 1.4% carbohydrate (weight/volume)] or basic formula with added sucrose, fructose, lactose, or glucose polymers, all as 13% (weight/ volume). All formulas were isocaloric. Their corresponding osmolalities were 278, 645, 1,130, 617, and 349 mOsmol/kg, respectively. Gastric evacuation of water-soluble materials from formulas containing sucrose, fructose, or glucose polymers was significantly slower than the gastric evacuation of the basic formula and the formula that contained lactose. The net fluid absorption from the small intestine was significantly greater from the basic and lactose-containing formulas when compared with sucrose, fructose, or glucose polymer-containing formulas. When the synthetic milk formulas were placed directly into the isolated jejunoileum in vivo, the formulas of higher osmolality (fructose, sucrose, and lactose) caused water flux into the intestine at 60 min, while digestion of the lactose formula reversed the water flux within 120 min. We conclude that the type of added sugar is a decisive factor in gastric evacuation, and that water flux into and out of the intestine is significantly affected by the osmolality and rate of digestion and absorption of the carbohydrate in the formula; these differences among sugars may play a significant role in the etiology of diarrhea.
D000818:Animals; D050260:Carbohydrate Metabolism; D003967:Diarrhea / Q000209:etiology*; D004040:Dietary Carbohydrates / Q000378:metabolism*; D005632:Fructose / Q000378:metabolism; D005746:Gastric Emptying; D005947:Glucose / Q000378:metabolism; D007408:Intestinal Absorption; D007419:Intestinal Secretions / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism*; D008892:Milk / Q000378:metabolism*; D009994:Osmolar Concentration; D051381:Rats; D011919:Rats, Inbred Strains; D013393:Sucrase / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism
D004040:Dietary Carbohydrates; D005632:Fructose; D013395:Sucrose; D013393:Sucrase; D005947:Glucose