The fluorimetric determination of the lecithin/sphingomyelin ratio of amniotic fluid after HPLC.
We have investigated whether fluorimetric determination of the lecithin/sphingomyelin ratio is feasible after HPLC. We found that the saturated phospholipid, dipalmitoyl lecithin, which is secreted by the fetal lung, and which cannot be detected by ultraviolet spectrophotometry, can be determined fluorimetrically. For this purpose we have added the fluorescence-enhancing substance, 1,6-diphenyl-1,3,5-hexatriene to the effluent. With the aid of a continuous-flow system we showed that the method can be used for a rapid assay of the ratio of these phospholipids in amniotic fluid.
D000653:Amniotic Fluid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005470:Fluorometry; D006801:Humans; D010713:Phosphatidylcholines / Q000032:analysis*; D011247:Pregnancy; D013109:Sphingomyelins / Q000032:analysis*
D010713:Phosphatidylcholines; D013109:Sphingomyelins