The value of combined determination of high molecular mass API and LP-X in the differential diagnosis of intrahepatic and extrahepatic obstruction.
Combined determination of serum lipoprotein-X and electrophoretic separation of high molecular mass (HMr) alkaline phosphatase has been proposed as a marker for the differential diagnosis between intrahepatic cholestasis and extrahepatic obstructive jaundice. Of 32 patients who were known to be lipoprotein-X positive and in whom a definitive diagnosis had been made, 13 had intrahepatic cholestasis and 17 extrahepatic obstruction, and 2 had both intrahepatic and extrahepatic obstruction. The detection of HMr alkaline phosphatase isoenzyme proved to be a sensitive and specific test for detecting liver disease, particularly obstructive liver disease. The diagnostic significance of the combined determination of serum lipoprotein-X to demonstrate or exclude cholestasis and electrophoretic separation of HMr alkaline phosphatase isoenzymes to allow differentiation between intrahepatic cholestasis and extrahepatic obstruction was investigated.
D000328:Adult; D000368:Aged; D000469:Alkaline Phosphatase / Q000097:blood*; D001651:Cholestasis, Extrahepatic / Q000175:diagnosis*; D002780:Cholestasis, Intrahepatic / Q000175:diagnosis*; D004796:Clinical Enzyme Tests; D003937:Diagnosis, Differential; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D008073:Lipoprotein-X / Q000097:blood*; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight
D007527:Isoenzymes; D008073:Lipoprotein-X; D000469:Alkaline Phosphatase