An in vitro epidermal slice technique for identifying chemicals with potential for severe cutaneous effects.
We have developed an in vitro model, using epidermal slices, to identify skin-corrosive potential. Such potential has been correlated with the ability to lyse stratum corneum, indicated by a lowered electrical resistance of the skin slice. Using 41 corrosive and 22 irritant chemicals, a comparison of in vitro and in vivo classifications has shown that this in vitro technique identifies corrosive agents with high precision. Therefore the model has considerable potential as a prescreen to conventional animal tests and, unlike in vivo screening tests, can provide both objective and quantifiable data.
D000818:Animals; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004553:Electric Conductivity; D066298:In Vitro Techniques; D007509:Irritants / Q000633:toxicity*; D008954:Models, Biological; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000187:drug effects*
D007509:Irritants