On the difference between colonic and small intestinal alkaline phosphatase.
Colonic and small intestinal alkaline phosphatase extracts were studied biochemically and electrophoretically to elucidate the source of a reported difference in cellulose acetate electrophoretic mobility. Both preparations were inactivated with 0.5 mmol/L L-phenylalanine but retained full activity in the presence of 1.0 mmol/L tetramisole. Treatment with neuraminidase changed a minor fraction of the small intestinal but the major portion of the colonic alkaline phosphatase to a cathodically migrating form. The most likely explanation for our findings is that the colon and small intestinal alkaline phosphatase are mixtures of the same multiple forms but in different proportions.
D000469:Alkaline Phosphatase / Q000302:isolation & purification / Q000378:metabolism*; D003106:Colon / Q000201:enzymology*; D004588:Electrophoresis, Cellulose Acetate; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology*; D007421:Intestine, Small / Q000201:enzymology*; D009928:Organ Specificity
D000469:Alkaline Phosphatase