Differential effects of Nd-YAG laser on collagen and elastin production by chick embryo aortae in vitro. Relevance to laser angioplasty for removal of atherosclerotic plaques.
Aortae from 17-day old chick embryos were subjected to irradiation with a Nd:YAG laser at energy densities varying from 1.2 - 4.7 X 10(3) J/cm2. The aortae were pulse-labeled in vitro with [3H]proline or [14C]valine, and the synthesis of collagenous polypeptides and soluble elastin was examined by SDS-polyacrylamide gel electrophoresis, followed by fluorography and quantitative scanning densitometry. Irradiation of the aortae with Nd:YAG laser resulted in inhibition of the synthesis of the extracellular matrix proteins. The production of collagen was inhibited to a considerably larger degree than the production of elastin. Thus, the biosynthetic pathway for collagen production appears to be more susceptible to laser inhibition than the corresponding pathway for elastin production. These observations may have relevance to laser angioplasty which has been proposed to be applicable for removal of atherosclerotic plaques in human vessels. Specifically, the results suggest that inhibition of the extracellular matrix production may result in weakening of the vessel wall with subsequent aneurysm formation and rupture.
D000818:Animals; D001011:Aorta / Q000196:embryology / Q000378:metabolism / Q000528:radiation effects*; D002642:Chick Embryo; D003094:Collagen / Q000096:biosynthesis*; D004549:Elastin / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D005353:Fibronectins / Q000096:biosynthesis; D007834:Lasers; D008970:Molecular Weight; D011392:Proline / Q000378:metabolism; D014633:Valine / Q000378:metabolism
D005353:Fibronectins; D003094:Collagen; D004549:Elastin; D011392:Proline; D014633:Valine