title: Schistosoma mansoni: effect of maintenance in vitro on the uptake and incorporation of leucine by adult worms.
abstract: The rates of leucine uptake and incorporation into protein by adult male and female Schistosoma mansoni were not affected by maintenance in vitro for up to 10 days' duration, despite the decline in the protein content of male and female worms of approximately 35 and 55%, respectively, during this period. The effect of maintenance in vitro was obscured in paired female schistosomes by the apparent shielding of the female tegument within the gynaecophoral canal of the male. Incorporation rates were reduced by 50% in the presence of 2 mM cycloheximide whereas uptake rates were unaffected. Adult schistosomes are unable to maintain their in vivo protein levels purely by recourse to exogenous amino acids absorbed across the tegument in vitro, and the rates of uptake and incorporation of leucine appear to reflect the changing somatic requirements of the worms and are probably not correlated with the reproductive activity of adult worms in vitro. The possible role of alimentary rather than tegumental nutrition in egg production in vivo is discussed.
mesh_terms: D000409:Alanine / Q000378:metabolism; D000818:Animals; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology; D005260:Female; D007930:Leucine / Q000378:metabolism*; D008297:Male; D014176:Protein Biosynthesis; D012098:Reproduction / Q000187:drug effects; D012550:Schistosoma mansoni / Q000378:metabolism* / Q000502:physiology; D013997:Time Factors
chemical_list: D003470:Culture Media; D003513:Cycloheximide; D007930:Leucine; D000409:Alanine