Antineurofilament antibody: an increased incidence in murine chronic measles encephalitis and the use of rat sciatic nerve sections as substratum.
Virus-associated autoimmune phenomena were investigated in the HNT-C57BL/6 chronic measles encephalitic mouse system. The incidence of serum antineurofilament autoantibodies was studied by an indirect immunofluorescence procedure using a FITC-conjugated goat anti-mouse immunoglobulin. Transversely cut cryostat sections of rat sciatic nerve post-treated with 1% Triton X-100 was used as substratum and had the advantages of increased reliability and reproducibility of neurofilament (NF) antibody titrations compared to the previously described longitudinal spinal cord sections. An increased incidence of anti-NF autoantibody was observed in sera from acutely, subacutely, chronically and asymptomatically infected mice compared to uninfected or mock-infected mice suggesting an association between the appearance of anti-NF antibody and HNT measles virus infection of the CNS.
D000818:Animals; D001323:Autoantibodies / Q000032:analysis*; D001921:Brain / Q000276:immunology; D003599:Cytoskeleton / Q000276:immunology*; D004660:Encephalitis / Q000276:immunology*; D008457:Measles / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D013116:Spinal Cord / Q000276:immunology
D001323:Autoantibodies