The localization of antibiotics by continuous sucrose gradient density.
Rat kidney was homogenized and centrifuged by continuous sucrose gradient density. Aminoglycosides and beta-lactam antibiotics were localized in the lysosomes, whereas the localization of tetracyclines was in the mitochondria. This study demonstrated a technique for the localization of antibiotics in the subcellular fractions without the use of a radioactive tracer.
D000617:Aminoglycosides / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism*; D007769:Lactams; D008247:Lysosomes / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000378:metabolism*; D013395:Sucrose
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D007769:Lactams; D011506:Proteins; D013395:Sucrose