Determination of bovine retinal melatonin with HPLC-EC.
Bovine retinae, devoid of pigment epithelium, were assayed for endogenous melatonin using high-performance liquid chromatography with electrochemical detection (HPLC-EC). A method of extracting the more polar biogenic amines and metabolites was employed, allowing for direct injection of retinal supernatants into the chromatographic column.


RESULTS
1) confirm the presence of melatonin in the bovine retina; 2) provide a "benchmark" of 3.01 ng/retina for normal adult retinae collected during photophase; and 3) indicate that HPLC-EC represents an adequately sensitive instrument for the detection and quantification of melatonin in a single bovine retina.
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry / Q000379:methods; D008550:Melatonin / Q000378:metabolism*; D012160:Retina / Q000378:metabolism*
D008550:Melatonin