Quantification of unscheduled DNA synthesis in mononuclear leukocytes of the horse.
This study compares the relationship between N-acetoxy-2-acetylaminofluorene (NA-AAF) and u.v. induced unscheduled DNA synthesis (UDS) and their respective relationships to age and blood pressure in horse mononuclear leukocytes with earlier, similar investigations on human leukocytes. U.v. induced UDS was found to proceed more rapidly than NA-AAF induced UDS. A pronounced lag period associated with the rapid demand for 3H-dThd into DNA after u.v. damage was observed. NA-AAF induced UDS correlated significantly with NA-AAF binding, age and the blood pressure of male horses. UDS values, induced by either method, were about half the level calculated for human leukocytes.
D000099:Acetoxyacetylaminofluorene / Q000494:pharmacology; D000818:Animals; D001794:Blood Pressure; D004261:DNA Replication / Q000187:drug effects / Q000528:radiation effects*; D006736:Horses / Q000378:metabolism*; D007700:Kinetics; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D013936:Thymidine / Q000097:blood; D014466:Ultraviolet Rays
D000099:Acetoxyacetylaminofluorene; D013936:Thymidine