Blockade of a motor response by cholinergic stimulation of the toad midbrain tegmentum.
Application of an acid solution to the dorsal skin of conscious toads having intact nervous system induces a scratching reflex and escape movements, as well as autonomic alterations (hypertension and tachycardia) that are part of the defense response. The motor components of this response are abolished or reduced by microinjection of 60, 30, 15 or 7.5 ng carbachol into the midbrain tegmentum. The cardiovascular components, however, continue to be present, although their amplitude is reduced. The depression of the motor response is statistically significant up to 15 minutes for the 60 ng dose, up to 10 minutes for the 15 and 30 ng doses, and only up to 5 minutes for the 7.5 ng dose. The data suggest that the midbrain tegmentum may modulate the reflex motor response triggered by a noxious stimulus and also participate in the organization of the escape movements. The importance of cholinergic agents in this modulation is discussed. The persistence of the cardiovascular component of the response shows the importance of this parameter as an indicator of alert situations.
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002025:Bufonidae; D002217:Carbachol / Q000494:pharmacology*; D004924:Escape Reaction / Q000187:drug effects; D005260:Female; D006339:Heart Rate / Q000187:drug effects; D006851:Hydrochloric Acid / Q000494:pharmacology*; D008297:Male; D008845:Microinjections; D009043:Motor Activity / Q000187:drug effects*; D012018:Reflex / Q000187:drug effects; D013681:Tegmentum Mesencephali / Q000502:physiology*
D002217:Carbachol; D006851:Hydrochloric Acid