Cytotoxic action of rat alveolar macrophages for ozone-exposed lung fibroblasts.
The objective of this study was to determine if exposure of cultured rat lung fibroblasts to O3 would trigger a subsequent attack by rat alveolar macrophages (AM). Fibroblasts labeled with [3H]proline (3HPR) were exposed to O3 (0, 0.74, 1.35, 2.46 or 4.48 ppm) for 1 h. AM were added (5:1, AM: fibroblast) and after 2, 24, 48, 76 and 96 h of co-culture, loss of 3HPR was measured as an index of AM-induced injury. Fibroblasts exposed to O3 and co-cultured with AM lost significantly more 3HPR than without AM co-culture. The results suggest that O3 injury to the lung may be mediated in part through an attack by AM upon O3-injured fibroblasts.
D000818:Animals; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D010126:Ozone / Q000633:toxicity*; D011392:Proline / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium
D014316:Tritium; D010126:Ozone; D011392:Proline