title: Use of plasma protein fractions as serum substitutes for in vitro cell culture.
abstract: Fractions produced during routine production of human plasma protein concentrates using cold ethanol precipitation have been tested for their ability to support animal cell growth in vitro. A mouse-mouse hybridoma cell line, ES-6, which secretes a monoclonal antibody (mab) to human group A red blood cell antigen, has been used as a model. Fraction 4-1 protein has been found to be effective at 0.5-1.0 g/l in RPMI 1640 and has supported growth of ES-6 for 18 months, both growth and mab. production being comparable with that achieved in 10% foetal calf serum. Fraction 4-1 protein has been shown to contain albumin, transferrin, alpha-1 antitrypsin and immunoglobulin. It is reported to be a source of somatomedin C. The immunoglobulin content can be reduced. Other mouse and rat hybridomas, human mab. producing lymphoblastoid cells, Namalva and HEP-2 cells have grown well in medium supplemented with Fraction 4-1 protein.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D001798:Blood Proteins / Q000302:isolation & purification*; D002455:Cell Division; D002460:Cell Line; D003470:Culture Media; D006801:Humans; D006825:Hybridomas / Q000166:cytology* / Q000276:immunology; D051379:Mice; D013242:Sterilization
chemical_list: D000911:Antibodies, Monoclonal; D001798:Blood Proteins; D003470:Culture Media