Human platelet fibrinogen: purification and hemostatic properties.
Conditions were developed in which 80% to 90% of platelet fibrinogen could be routinely purified in nondegraded form from the fluid phase of platelet suspensions stimulated with the calcium ionophore, A23187, in the presence of calcium, leupeptin, and prostaglandin E1. Fibrinogen was separated from other released proteins by chromatography on diethylaminoethanol (DEAE)-cellulose using a continuous pH and ionic strength gradient. Purified platelet fibrinogen, greater than 98% homogeneous by immunoelectrophoresis and sodium-dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), consisted of intact A alpha, B beta and gamma A chains, but not gamma' chains, and was 95% to 96% clottable. Platelet fibrinogen was shown to compete for the binding of radiolabeled plasma fibrinogen to ADP-activated platelets in a manner identical to that of unlabeled plasma fibrinogen itself. Also, at equivalent protein concentrations, platelet and plasma fibrinogens supported platelet aggregation to an equivalent extent. Based upon these results, we conclude that there is no significant difference between platelet and plasma fibrinogen with respect to their size, their clottability, their affinity for the activated platelet fibrinogen receptor, or their capacity to support subsequent platelet aggregation.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D001667:Binding, Competitive; D001777:Blood Coagulation; D001792:Blood Platelets / Q000032:analysis*; D001798:Blood Proteins / Q000378:metabolism; D003594:Cytoplasmic Granules; D004591:Electrophoresis, Polyacrylamide Gel; D005340:Fibrinogen / Q000302:isolation & purification*; D006487:Hemostasis; D006801:Humans; D010974:Platelet Aggregation / Q000187:drug effects; D012967:Sodium Dodecyl Sulfate
D001798:Blood Proteins; D012967:Sodium Dodecyl Sulfate; D000244:Adenosine Diphosphate; D005340:Fibrinogen