Bromodeoxyuridine treatment of rat liver cells in culture induces an increased metabolic conversion of exogenous ornithine.
In the presence of bromodeoxyuridine, rat liver cells show an increased incorporation of radiolabeled ornithine into cellular proteins. This increase was not due to activation of urea cycle enzymes but rather to an increase in metabolism of ornithine. Furthermore, treatment with bromodeoxyuridine changes profoundly and selectively the size of the free amino acid pool in these cells.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001345:Autoradiography; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D004848:Epithelium / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D009952:Ornithine / Q000378:metabolism*; D051381:Rats
D000596:Amino Acids; D003513:Cycloheximide; D009952:Ornithine; D001973:Bromodeoxyuridine