Isolation of glomeruli from areas of bovine cerebellum and comparison of [3H]serotonin uptake.
A method for bulk preparation of glomerular particles from subdivisions of the bovine cerebellum is presented. This method represents a modification of that previously reported by Hajos et al. [8], which increases the yield of glomerular protein by five-fold (5.5 mg/g wet wt) without compromising structural integrity or homogeneity. In addition, it offers the advantage of allowing one to study intraregional variations in the metabolic properties of cerebellar glomeruli. [3H]Serotonin (5-HT) uptake was measured in this preparation and it was demonstrated that glomeruli possess an active high affinity mechanism for this substrate. Comparison of [3H]5-HT uptake by glomeruli isolated from the cerebellar cortices of the lateral hemispheres and vermis revealed no differences in their kinetic properties.
D000818:Animals; D002417:Cattle; D002458:Cell Fractionation; D002531:Cerebellum / Q000378:metabolism / Q000648:ultrastructure*; D007700:Kinetics; D012701:Serotonin / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism / Q000648:ultrastructure
D012701:Serotonin