title: Sensitive liquid chromatographic method for physostigmine in biological fluids using dual-electrode electrochemical detection.
abstract: A liquid chromatographic method using dual-electrode detection has been developed for determination of physostigmine in biological fluids. The limit of detection is in the order of 25-50 pg mol-1 of plasma. A high sample throughput is obtained by a single solvent extraction step and autoinjection into the chromatograph. Data following oral doses of physostigmine are presented.
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008297:Male; D010084:Oxidation-Reduction; D010830:Physostigmine / Q000031:analogs & derivatives / Q000032:analysis* / Q000097:blood / Q000378:metabolism / Q000652:urine; D010949:Plasma / Q000032:analysis; D011048:Polarography; D013997:Time Factors
chemical_list: C026718:physostigmine salicylate; D010830:Physostigmine