title: Effects of retinoids on invasion of organ cultures of chick chorioallantoic membrane by adenovirus transformed cells.
abstract: Invasion of chick chorioallantoic membrane (CAM) organ cultures by rat 3Y1 cells transformed by the highly oncogenic human adenovirus type 12 (3Y1/12-10 cells) was inhibited by several retinoids tested. The anti-invasive activity of the retinoids was dependent on retinoid concentration and continuous (4 d) exposure of the CAM. The 50% retinoid dose (dose effective in achieving a response in half of the organ cultures) that inhibited invasion was 0.85 micrograms/ml of retinol palmitate, 0.39 micrograms/ml of retinoic acid, or 0.16 micrograms/ml of retinol acetate. This dose was of the same order of magnitude as that which induced CAM differentiation, and was three- to fourfold less than the dose that caused cytotoxic damage of CAM. In addition, the retinoids inhibited 3Y1/12-10 cell growth by approximately 40% at levels over 10-fold higher than those needed for anti-invasion activity. The findings suggest that the anti-invasive activity of retinoids was at least partly due to direct induction of cell differentiation of the CAM host tissue.
mesh_terms: D000260:Adenoviruses, Human / Q000502:physiology; D000482:Allantois / Q000166:cytology; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000473:pathology*; D002472:Cell Transformation, Viral; D002642:Chick Embryo; D002823:Chorion / Q000166:cytology; D004224:Diterpenes; D005321:Extraembryonic Membranes / Q000166:cytology; D009361:Neoplasm Invasiveness; D009924:Organ Culture Techniques; D051381:Rats; D012176:Retinoids / Q000494:pharmacology*; D000084562:Retinyl Esters; D014212:Tretinoin / Q000494:pharmacology; D014801:Vitamin A / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D004224:Diterpenes; D012176:Retinoids; D000084562:Retinyl Esters; D014801:Vitamin A; C014794:retinol palmitate; C009166:retinol acetate; D014212:Tretinoin