title: Location of the maltosyl isothiocyanate binding site on the human erythrocyte glucose transporter.
abstract: The covalent affinity probe maltosyl isothiocyanate (MITC) has been used previously to identify the glucose transporter of human erythrocytes as a component of band 3. By use of limited proteolysis, the site on the Mr 100 000 protein to which MITC attaches has been localized to a 17 000-dalton region near the center of the polypeptide chain which is intimately associated with the membrane. The erythrocyte anion transporter, which is probably homologous to the glucose carrier, has a corresponding segment which is known to bind the covalent affinity label 4,4'-diisothiocyano-2,2'-stilbenedisulfonic acid [Ramjeesingh, M., Gaarn, A., & Rothstein, A. (1980) Biochim. Biophys. Acta 559, 127-139]. These results suggest that, in addition to having structural features in common, the two carrier proteins may be quite similar with regard to functional organization.
mesh_terms: D000345:Affinity Labels / Q000378:metabolism*; D001665:Binding Sites; D001786:Blood Glucose / Q000378:metabolism*; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002918:Chymotrypsin; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006801:Humans; D017879:Isothiocyanates; D007700:Kinetics; D008970:Molecular Weight; D009004:Monosaccharide Transport Proteins; D010446:Peptide Fragments / Q000032:analysis; D013861:Thiocyanates / Q000097:blood*
chemical_list: D000345:Affinity Labels; D001786:Blood Glucose; D002352:Carrier Proteins; D017879:Isothiocyanates; D009004:Monosaccharide Transport Proteins; D010446:Peptide Fragments; D013861:Thiocyanates; C024937:maltosyl isothiocyanate; D002918:Chymotrypsin