The production of 1,2-propanediol in ethanol treated rats.
Chronic administration of ethanol in the most commonly used experimental diet (Lieber, C. S., and DeCarli, L. M. (1976) Fed. Proceed. 35, 1232-1236) resulted in the production of 1,2-propanediol within one week of initiation of alcohol feeding. After two weeks 1,2-propanediol levels were 8.8 +/- 1.6 nmol/ml in alcohol treated animals. No 1,2-propanediol was apparent in pair fed control animals at any time during this study. Consistent with the proposed mechanism of production of 1,2-propanediol in acetone treated rats (Casazza, J. P., Felver, M. E., and Veech, R. L. (1984) J. Biol. Chem. 259, 231-236), both liver acetone and acetol monooxygenase activities and blood beta-hydroxybutyrate were elevated in ethanol treated animals. Acetone and acetol monooxygenase activities were 0.118 +/- 0.016 and 0.110 +/- 0.016 umol/min/g liver after two weeks of ethanol treatment. Acetone and acetol monooxygenase activities in pair fed controls were 0.016 +/- 0.002 and 0.015 +/- 0.002 umol/min/g liver. beta-Hydroxybutyrate levels were highest after one week of treatment; 1.64 +/- 0.12 umol/ml in ethanol treated rats and 0.16 +/- 0.02 umol/ml in pair fed controls. Throughout this study serum acetol and 2,3-butanediol were less than the detection limits of these assays (less than 5 nmol/ml).
D020155:3-Hydroxybutyric Acid; D000437:Alcoholism / Q000378:metabolism*; D000818:Animals; D003577:Cytochrome P-450 Enzyme System; D004790:Enzyme Induction; D006801:Humans; D006885:Hydroxybutyrates / Q000097:blood; D008862:Microsomes, Liver / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000096:biosynthesis; D019946:Propylene Glycol; D011409:Propylene Glycols / Q000097:blood*; D051381:Rats; D013997:Time Factors
D006885:Hydroxybutyrates; D011409:Propylene Glycols; D019946:Propylene Glycol; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; C045704:acetone monooxygenase; D020155:3-Hydroxybutyric Acid