Preparation of monoclonal antibody to sulfatoxygalactosylglycerolipid by in vitro immunization with a glycolipid-glass conjugate.
A monoclonal antibody to the testicular sulfatoxygalactosylglycerolipid has been raised following in vitro stimulation of lymphocytes with glycolipid immobilized on glass beads by means of a photoactivatable heterobifunctional crosslinking agent. The antibody can distinguish between glycerol and sphingosine-based sulfoglycolipids.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002417:Cattle; D005260:Female; D005898:Glass; D006017:Glycolipids / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007075:Immunoglobulin M / Q000096:biosynthesis; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013433:Sulfoglycosphingolipids / Q000276:immunology*; D013737:Testis / Q000276:immunology
D000911:Antibodies, Monoclonal; D006017:Glycolipids; D007075:Immunoglobulin M; D013433:Sulfoglycosphingolipids