title: Histochemical demonstration of monoaminergic and cholinesterase-positive nerve fibres in regenerating rat submandibular gland autografts.
abstract: A cholinesterase localization method and a monoamine histofluorescence technique were used to locate nerve fibres in regenerating rat submandibular gland autografts. Experimental rats had a portion of one submandibular gland excised and cut into small fragments which were autografted immediately into the middle one-third of the tongue. Control rats had a portion of one submandibular gland removed and discarded, and their tongues were sham-operated. Seven to ten weeks later, the rats were killed and the tongues were removed, frozen and sectioned in a cryostat. A light microscopical study of the tongue sections subjected to the cholinesterase technique showed that the submandibular gland autografts contained many nerve fibres that exhibited cholinesterase activity. These cholinesterase-positive nerve fibres were distributed throughout the autografts. The fibres were associated with the numerous duct-like structures and the less numerous acini. In addition, ultraviolet illumination of tongue sections after treatment with a glyoxylic acid mixture revealed histofluorescent monoaminergic nerves within the autografts. These fibres were less prominent than the cholinesterase-positive fibres and appeared to run primarily along blood vessels within the autografts. The results suggest that autonomic nerves are present within regenerating submandibular gland autografts.
mesh_terms: D000818:Animals; D001341:Autonomic Nervous System / Q000502:physiology; D002802:Cholinesterases / Q000378:metabolism; D006651:Histocytochemistry; D008297:Male; D009412:Nerve Fibers / Q000378:metabolism*; D009416:Nerve Regeneration; D051381:Rats; D011919:Rats, Inbred Strains; D012038:Regeneration; D013363:Submandibular Gland / Q000294:innervation* / Q000502:physiology / Q000637:transplantation; D014182:Transplantation, Autologous
chemical_list: D002802:Cholinesterases