[Immunochemical study of blastolysin].
The immunochemical properties of blastolysin (BL), a preparation obtained from the cell walls of Lactobacillus bulgaricus with immunostimulating and antitumor activity were studied. The experiments on dogs and rabbits showed that the immunogenic effect of BL was not high. However, after parenteral administration to the dogs and rabbits it induced production of the specific antibodies detected with the Ouchterlony test of direct hemagglutination and immunodiffusion. This should be taken into account in development of the schemes for its use as a pharmacological agent. With the procedures of gel filtration on the columns with Sephadex G-75 and immunodiffusion the fraction composition of BL was determined and the use of the immunochemical procedures for standardization of the BL preparations was shown to be possible.
D000818:Animals; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic / Q000276:immunology*; D000906:Antibodies / Q000032:analysis; D000918:Antibody Specificity; D004285:Dogs; D004306:Dose-Response Relationship, Immunologic; D006020:Glycopeptides / Q000276:immunology; D007114:Immunization / Q000379:methods; D007120:Immunochemistry; D011817:Rabbits; D013997:Time Factors
D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D000906:Antibodies; D006020:Glycopeptides; C025182:blastolysin
Immunokhimicheskoe izuchenie blastolizina.