Methods for chromatofocusing of cerebrospinal fluid and serum immunoglobulin G.
Chromatofocusing programs were designed for separations of submilligram amounts of normal and abnormal human IgG. The Pharmacia FPLC system, equipped with a Mono P column or a specially designed, small column was used for the separations. Normal IgG in paired cerebrospinal fluid and serum samples, paired samples from patients with intrathecal immunoglobulin G synthesis, as well as sera with IgG M components were examined. Abnormal immunoglobulin G components, especially those with pI values greater than ca. 7.0 pH units, were easily identified.
D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000134:cerebrospinal fluid; D007525:Isoelectric Focusing; D013056:Spectrophotometry, Ultraviolet
D007074:Immunoglobulin G