Induction, isolation and a characterization of the lipid content of plasma vitellogenin from two Salmo species: rainbow trout (Salmo gairdneri) and sea trout (Salmo trutta).
Vitellogenin synthesis is induced in juvenile rainbow trout (Salmo gairdneri) and juvenile sea trout (Salmo trutta) by estradiol-17 beta. A purification procedure for vitellogenin from trout plasma by precipitation with MgCl2-EDTA and subsequent anion exchange chromatography on DEAE-Sephacel is described. The total lipid contents of purified rainbow trout and sea trout vitellogenins are 18 and 19%, respectively. Approximately 2/3 of the lipids are phospholipids, while the remainder consists of triglycerides and cholesterol. Phosphorus determinations on delipidated vitellogenin yield a phosphorus content of 0.63% in rainbow trout and 0.58% in sea trout vitellogenin. Native (dimeric) vitellogenins from rainbow trout and sea trout both have an apparent molecular weight of 440,000, when estimated by gel filtration on Sepharose 6B.
D000818:Animals; D004958:Estradiol / Q000494:pharmacology; D008055:Lipids / Q000097:blood*; D008970:Molecular Weight; D010758:Phosphorus / Q000032:analysis; D012487:Salmonidae / Q000097:blood*; D013045:Species Specificity; D014337:Trout / Q000097:blood*; D014819:Vitellogenins / Q000097:blood* / Q000302:isolation & purification
D008055:Lipids; D014819:Vitellogenins; D010758:Phosphorus; D004958:Estradiol