Differentiation of Abelson murine leukemia virus-infected promonocytic leukemia cells.
Virus-producing, tumorigenic, promonocytic leukemia cell lines were derived from Abelson murine leukemia virus-infected mice. This study shows that, of these 25 cloned lines, 22 were capable of extensive differentiation. It also shows that granulocyte-macrophage colony-stimulating activity increased the proportion of differentiating cells in 17/22 lines. Cells from agar colonies with a diffuse colony morphology had an increased expression of mature macrophage phenotypic characteristics, and a reduced proliferative capacity in vitro, compared to cells from agar colonies with a compact colony morphology. Cells from diffuse colonies also produced less Abelson virus, and were less tumorigenic in vivo than cells from compact colonies. Together, these results suggest some Abelson virus-producing leukemic cells are not blocked in their capacity to differentiate and are capable of reversing the transformed phenotype.
D000011:Abelson murine leukemia virus / Q000502:physiology; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D002999:Clone Cells; D003114:Colony-Forming Units Assay; D007942:Leukemia, Experimental / Q000097:blood* / Q000382:microbiology; D008264:Macrophages / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000382:microbiology / Q000473:pathology*; D014779:Virus Replication