Isolation and identification of delta 6-lithocholenic acid (3 alpha-hydroxy-5 beta-6-cholen-24-oic acid) as an intestinal bacterial metabolite of chenodeoxycholic acid in man.
Fecal bacterial biotransformation studies of chenodeoxycholic acid were performed. Incubations were carried out for 30-s to 12-h time intervals. delta 6-Lithocholenic acid was isolated by thin-layer chromatography. Its structure was confirmed by gas-liquid chromatography and mass spectrometry. The proportion of chenodeoxycholic acid biotransformed to delta 6-lithocholenic acid consistently ranged from 5.5 to 14.0%.
D000332:Aerobiosis; D001419:Bacteria / Q000378:metabolism*; D001711:Biotransformation; D002250:Carbon Radioisotopes; D002635:Chenodeoxycholic Acid / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D005243:Feces / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D007700:Kinetics; D008095:Lithocholic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000302:isolation & purification
D002250:Carbon Radioisotopes; D002635:Chenodeoxycholic Acid; C046457:3-hydroxy-6-cholen-24-oic acid; D008095:Lithocholic Acid