A methacrylate embedding technique for combined autoradiography and acid phosphatase histochemistry.
A method is described that enables the simultaneous application of autoradiography and histochemistry in tissue sections prepared using a hydroxyethyl methacrylate (HEMA) embedding medium. A novel fixation regime, using a 1:9 v/v mixture of acetone and 10% neutral buffered formalin, improves section quality, histological staining and reduces tissue and cell shrinkage. The combined localization of [6-3H] thymidine incorporation and acid phosphatase in mouse thymus, duodenum, human stomach biopsy, earthworm and Walker tumour is described and counts of macrophages, phagocytosed cells, pyknotic cells, mitotic figures and thymidine-incorporating cells from young and old mouse thymus are presented.
D000135:Acid Phosphatase / Q000032:analysis*; D000179:Acrylates; D000818:Animals; D001345:Autoradiography / Q000379:methods*; D002455:Cell Division; D005404:Fixatives; D006801:Humans; D008297:Male; D008689:Methacrylates; D051379:Mice
D000179:Acrylates; D005404:Fixatives; D008689:Methacrylates; D000135:Acid Phosphatase