On the distribution of ribosome-inactivating proteins amongst plants.
The extracts from various parts (mostly seeds) of 56 different plants were examined for inhibition of protein synthesis by a rabbit reticulocyte lysate. Most extracts inhibited protein synthesis with an ID50 (concentration giving 50% inhibition) of 100 micrograms extract protein per ml, or less. The extracts with high activity were partially purified by CM cellulose chromatography. Protein-containing fractions were separated which inhibited protein synthesis and resembled the ribosome-inactivating proteins from plants previously described. Thus, ribosome-inactivating proteins appear to be virtually ubiquitous in plants.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D010448:Peptide Initiation Factors / Q000032:analysis*; D010936:Plant Extracts / Q000494:pharmacology; D010940:Plant Proteins / Q000494:pharmacology*; D010944:Plants / Q000032:analysis*; D039665:Prokaryotic Initiation Factor-3; D014176:Protein Biosynthesis; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D012270:Ribosomes / Q000187:drug effects*
D010448:Peptide Initiation Factors; D010936:Plant Extracts; D010940:Plant Proteins; D039665:Prokaryotic Initiation Factor-3