Synergism of vitamins A and C on fibrinolysis.
A hitherto unknown synergism exerted by retinol (vitamin A) and L-ascorbic acid (vitamin C) was discovered using endothelial cells. Retinol stimulated the extracellular and intracellular activities of plasminogen activator up to approximately 8- and 4-fold from the control values, respectively. L-Ascorbic acid enhanced the extracellular and intracellular activities up to approximately 1.5-fold. Above all it was demonstrated that their effects on extracellular activity were synergistic; simultaneous administration of these two vitamins enhanced the extracellular activity up to a 20- to 50-fold. Synthesis of plasminogen activator induced with vitamins A and C was inhibited by a protein synthesis inhibitor, cycloheximide.
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D002417:Cattle; D002478:Cells, Cultured; D004357:Drug Synergism; D004727:Endothelium / Q000187:drug effects*; D005110:Extracellular Space / Q000201:enzymology; D005342:Fibrinolysis / Q000187:drug effects*; D010960:Plasminogen Activators / Q000096:biosynthesis*; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism; D014801:Vitamin A / Q000494:pharmacology*
D014801:Vitamin A; D010960:Plasminogen Activators; D014568:Urokinase-Type Plasminogen Activator; D001205:Ascorbic Acid