High electroendosmotic agarose electrophoresis and nitrocellulose immobilisation for the detection of oligoclonal bands in unconcentrated cerebrospinal fluid.
A sensitive (1 microL of unconcentrated sample), specific (IgG Fc) and economical (10 p/test) method for the detection of oligoclonal bands in cerebrospinal fluid is presented. Using commonly available technology (agarose gel electrophoresis) and giving results within 6 h, the approach used here is a powerful tool in the investigation of immunoglobulin abnormalities in many biological fluids.
D003101:Collodion; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005188:False Negative Reactions; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000134:cerebrospinal fluid*; D007128:Immunoglobulin Fragments / Q000134:cerebrospinal fluid*; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid*; D007525:Isoelectric Focusing; D008832:Microchemistry
D007141:Immunoglobulin Fc Fragments; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; D003101:Collodion