Studies on chromosome aberrations in the eggs of mice fertilized in vitro after irradiation. I. Chromosome aberrations induced in sperm after X-irradiation.
The induction of chromosome aberrations in eggs of mice fertilized with X-irradiated sperm was performed by using an in vitro fertilization technique. Capacitated mature sperm was irradiated with various doses of X-rays and cytological analysis of the first cleavage metaphase of in vitro fertilized eggs was made. The frequencies of chromosome aberrations increased exponentially with dose and the dose-response relationship for overall breaks fitted well to a quadratic equation. The chromosome aberrations were mainly chromosome-type (82.1%), and the majority of aberrations were fragments.
D000818:Animals; D002869:Chromosome Aberrations; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005306:Fertilization; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010063:Ovum / Q000648:ultrastructure; D013094:Spermatozoa / Q000528:radiation effects*; D014965:X-Rays