Heterogeneity of cell lines derived after transformation of early passage rodent cells by the Ha-ras1 human oncogene.
The chromosome patterns of Chinese hamster cell lines derived after immortalization or tumorigenic conversion of early passage cells with recombinants carrying the mutated T24 or the normal human Ha-ras1 gene have been characterized by trypsin-Giemsa banding. Whereas immortalized Chinese hamster cell lines exhibited a near normal karyotype, tumorigenic cell lines were found to have abnormal karyotypes carrying marker chromosomes. Moreover, chromosomal patterns correlated with growth in semisolid media and tumourigenicity in nude mice. Similarly, malignant conversion of early passage Syrian hamster cells, with a recombinant carrying the mutated T24 human Ha-ras1 gene, resulted in cells with a near diploid karyotype. On the other hand, tumorigenic conversion of early passage Wistar rat cells with the same oncogene produced cell lines with heteroploid karyotypes. More chromosomal alterations have been observed during further growth of these cells. It is suggested that the transformed phenotype in these cells may be dependent on the chromosomal instability.
D000818:Animals; D002460:Cell Line; D002875:Chromosomes; D006224:Cricetinae; D006801:Humans; D007621:Karyotyping; D008647:Mesocricetus; D051379:Mice; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000235:genetics; D009857:Oncogenes; D051381:Rats; D011919:Rats, Inbred Strains; D011995:Recombination, Genetic; D014162:Transfection; D014170:Transformation, Genetic