[Comparative evaluation of several noninstrumental methods of determining fats in the fermentation media in manufacturing antibiotics].
The use of instrumental analysis (IR spectrometry, high performance liquid chromatography) for determination of fats in the nutrient media and fermentation broths in antibiotic production is not efficient. 7 noninstrumental methods for determination of fats in the fermentation broths of some fat-soluble polyester antibiotics were compared. The first method includes extraction of the fermentation broth with different agents, gravimetric estimation, measuring of the saponification number and refraction coefficient and thin-layer chromatography. It was shown that determination of the saponification number after extraction with alkanes or diethyl ether provided reliable results. Thin-layer chromatography can be used as a rapid method.
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D003470:Culture Media / Q000032:analysis; D005069:Evaluation Studies as Topic; D005285:Fermentation; D008055:Lipids / Q000032:analysis*; D008722:Methods; D012995:Solubility
D000900:Anti-Bacterial Agents; D003470:Culture Media; D008055:Lipids
Sravnitel'naia otsenka neskol'kikhh neinstrumental'nykh metodov opredeleniia zhirov v fermentatsionnykh sredakh pri proizvodstve antibiotikov.