Enzymatic correction of interference in the kinetic Jaffé reaction for determining creatinine in plasma.
We evaluated a simple, specific method for measuring plasma creatinine concentration, involving enzymatic degradation of creatinine before analysis by a kinetic Jaffé reaction. The enzymes--prepared in a dry, stable form--are convenient to use and eliminate the need to correct for sample dilution. As little as 50 microL of plasma is required, making the procedure ideal for use with samples from children. Between-day precision studies for determination of plasma creatinine at concentrations of 70 and 470 mumol/L yielded CVs of 7.8 and 4.3%, respectively. Analytical recovery of creatinine added to plasma samples ranged from 94 to 113%. Linear regression analysis demonstrated excellent agreement between results by this method and those by a "high performance" liquid-chromatographic technique.
D000581:Amidohydrolases; D002851:Chromatography, High Pressure Liquid; D003404:Creatinine / Q000097:blood*; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D008832:Microchemistry; D014512:Ureohydrolases
D007202:Indicators and Reagents; D003404:Creatinine; D000581:Amidohydrolases; C019946:creatininase; D014512:Ureohydrolases; C019944:creatinase