Association between progesterone binding and cytochrome P-450 content of hepatic microsomes in the rat treated with cobalt-haem.
The effect of cobalt protoporphyrin IX (Co-haem) given to male rats in single subcutaneous doses (25-100 mumol/kg body wt.) was studied. Co-haem decreased cytochrome P-450 content and aminopyrine N-demethylase activity, but increased progesterone content and 3H-progesterone binding in a dose-related manner. The effect of a single dose of 50 mumol/kg body wt. was reversible; cytochrome P-450 and progesterone content, and progesterone binding, returned to the normal level 24-40 d after injection but aminopyrine N-demethylase activity was only partially restored. The converse actions of Co-haem on microsomal progesterone and cytochrome P-450 content showed high correlation.
D000633:Aminopyrine N-Demethylase / Q000037:antagonists & inhibitors / Q000378:metabolism; D000818:Animals; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004790:Enzyme Induction / Q000187:drug effects; D006418:Heme / Q000378:metabolism; D007279:Injections, Subcutaneous; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D011166:Porphyrins / Q000494:pharmacology*; D011374:Progesterone / Q000378:metabolism*; D011524:Protoporphyrins / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344; D013379:Substrate Specificity
D065607:Cytochrome P-450 Enzyme Inhibitors; D011166:Porphyrins; D011524:Protoporphyrins; D006418:Heme; D011374:Progesterone; C007095:cobaltiprotoporphyrin; D003577:Cytochrome P-450 Enzyme System; D000633:Aminopyrine N-Demethylase