High-performance liquid chromatographic analysis of citric acid, inositol and fructose in boar and bull seminal plasma.
The efficiency of ultrafiltration and high-performance liquid chromatography (HPLC) in the routine analysis of dialysable sugar alcohols, organic acids and carbohydrates in boar and bull seminal plasma is evaluated. Permitting a simple and rapid separation of protein-free samples, ultrafiltration reduces the time of HPLC analysis by 40 minutes as compared to protein precipitation by means of methanol. Under optimal flow-conditions the separation of citric acid, inositol and fructose is achieved in 16 minutes. Moreover, the use of silicon-oil for separating seminal plasma within seconds is described.
D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002951:Citrates / Q000032:analysis*; D019343:Citric Acid; D005632:Fructose / Q000032:analysis*; D007294:Inositol / Q000032:analysis*; D008297:Male; D000432:Methanol; D012661:Semen / Q000032:analysis*; D012825:Silicon; D013552:Swine; D013997:Time Factors; D014462:Ultrafiltration
D002241:Carbohydrates; D002951:Citrates; D019343:Citric Acid; D005632:Fructose; D007294:Inositol; D000432:Methanol; D012825:Silicon