A novel oxidation of the carcinogen N-hydroxy-N-2-fluorenylacetamide catalyzed by peroxidase/H2O2/Br-.
N-Hydroxy-N-2-fluorenylacetamide, a proximate carcinogenic metabolite of N-2-fluorenylacetamide, is oxidized largely to 2-nitrosofluorene by lactoperoxidase or extract of peroxidative activity of rat uterus in an H2O2- and Br- -dependent reaction. Evidence is presented that the oxidizing species includes OBr- (HOBr). This novel oxidation may be involved in carcinogenesis by N-arylhydroxamic acids.
D015073:2-Acetylaminofluorene / Q000031:analogs & derivatives*; D000818:Animals; D001965:Bromides / Q000494:pharmacology*; D005260:Female; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D006881:Hydroxyacetylaminofluorene / Q000378:metabolism*; D007700:Kinetics; D007784:Lactoperoxidase / Q000378:metabolism*; D009603:Nitroso Compounds / Q000378:metabolism; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014599:Uterus / Q000201:enzymology*
D001965:Bromides; D009603:Nitroso Compounds; C012458:2-nitrosofluorene; D006881:Hydroxyacetylaminofluorene; D015073:2-Acetylaminofluorene; D006861:Hydrogen Peroxide; D007784:Lactoperoxidase; D010544:Peroxidases