title: Cytochemical localization of alkaline phosphatase in the cell membrane of Dictyostelium discoideum amebae.
abstract: We demonstrated that alkaline phosphatase was localized on the cell membrane of Dictyostelium discoideum amebae and on isolated plasma membranes. The enzyme activity was specifically inhibited by 0.01 M KCN or cysteine. The same method could also be applied to baker's yeast and MDCK cells (dog kidney cells in vitro).
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure; D004023:Dictyostelium / Q000201:enzymology* / Q000648:ultrastructure; D006651:Histocytochemistry; D008854:Microscopy, Electron
chemical_list: D000469:Alkaline Phosphatase