Genes controlling gp25/30 cell-surface molecules map to chromosomes X and Y and escape X-inactivation.
The monoclonal antibody AbO13 defines a cell-surface antigen that is expressed on most cultured human cells, but not on rodent cells. AbO13 precipitates glycoproteins of 25,000 and 30,000 mol. wt. from lysates of [3H]glucosamine-labeled human cells. Results of the serological typing of a panel of 25 rodent-human somatic cell hybrid clones show that reactivity with AbO13 segregates with the human X and Y chromosomes. The presence of either of these chromosomes is sufficient for O13 expression on the hybrid cell surface. Analysis of hybrid clones containing human X chromosomes with karyotypically defined deletions permitted the regional assignment of the X-linked gene locus controlling the expression of O13 to Xp22-pter. In addition, AbO13 is reactive with Chinese hamster-human hybrids derived from fibroblasts of a 49,XXXXX individual that contained only inactivated copies of the human X chromosome. These results suggest that the X-linked locus determining the expression of O13 is not subject to X-inactivation.
D000017:ABO Blood-Group System / Q000235:genetics; D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000235:genetics*; D002460:Cell Line; D002871:Chromosome Banding; D002874:Chromosome Mapping; D006224:Cricetinae; D003412:Cricetulus; D004303:Dosage Compensation, Genetic; D005260:Female; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D007621:Karyotyping; D008297:Male; D008970:Molecular Weight; D014960:X Chromosome; D014998:Y Chromosome
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D006023:Glycoproteins