The radioallergosorbent test (RAST) for the serological diagnosis of human anisakiasis.
The serum of patients with parasitologically confirmed and one patient with a clinically presumptive case of anisakiasis were tested by the radioallergosorbent test (RAST) and counter-electrophoresis (CEP) for IgE and IgG antibodies, respectively, using antigens prepared from larval (L3) stage Anisakis simplex and larval (L2) stage Toxocara canis. All sera were RAST-positive to the A. simplex antigen and RAST-negative to the T. canis antigen. All sera were CEP-negative to both antigens. The presence of a specific IgE antibody suggests that the RAST could serve as a useful technique for the serodiagnosis of anisakiasis in man.
D000328:Adult; D001199:Ascaridoidea; D003378:Counterimmunoelectrophoresis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009349:Nematode Infections / Q000175:diagnosis* / Q000276:immunology; D011852:Radioallergosorbent Test