Hybridoma cultivation in defined serum-free media: growth-supporting substances. I. Transferrin.
Effect of transferrin and effect of transferrin omission on growth of hybridomas in serum-free medium were studied. Transferrin at concentration 0.5 microgram/ml and higher concentrations stimulated growth of hybridoma cells. The optimum growth-supporting concentration of transferrin was 5 micrograms/ml. Omission of transferrin led to death of hybridoma T3-03 cells within 3-4 days. Omission of transferrin for a period of only 24 h damaged cells so severely, that they were unable to recover fully.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology*; D046508:Culture Techniques / Q000379:methods; D006825:Hybridomas / Q000187:drug effects*; D051379:Mice; D014168:Transferrin / Q000494:pharmacology*
D003470:Culture Media; D014168:Transferrin