Subcellular fractionation of Hymenolepis diminuta with special reference to the localization of marker enzymes.
A method for subcellular fractionation of Hymenolepis diminuta using whole worm homogenization and differential centrifugation is presented. Different fractions obtained in this study were screened for the presence of enzymes that serve as markers for plasma membrane, brush border, mitochondria, Golgi complex, endoplasmic reticulum, peroxisomes, lysosomes and cytosol. The purity of fractions was also monitored by transmission electron microscopy. The purity of fractions, particularly the brush border membranes, are compared to those obtained by previous methods for H. diminuta or other tissues.
D000818:Animals; D002458:Cell Fractionation; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure; D002467:Cell Nucleus / Q000032:analysis / Q000648:ultrastructure; D002498:Centrifugation; D003600:Cytosol / Q000201:enzymology / Q000648:ultrastructure; D004247:DNA / Q000032:analysis; D006056:Golgi Apparatus / Q000201:enzymology / Q000648:ultrastructure; D006867:Hydrolases / Q000032:analysis; D006926:Hymenolepis / Q000201:enzymology / Q000648:ultrastructure*; D008830:Microbodies / Q000201:enzymology / Q000648:ultrastructure; D008854:Microscopy, Electron; D008861:Microsomes / Q000201:enzymology / Q000648:ultrastructure; D008871:Microvilli / Q000201:enzymology / Q000648:ultrastructure; D008928:Mitochondria / Q000201:enzymology / Q000648:ultrastructure; D010088:Oxidoreductases / Q000032:analysis; D013347:Subcellular Fractions / Q000201:enzymology / Q000648:ultrastructure
D004247:DNA; D010088:Oxidoreductases; D006867:Hydrolases