Fluorescent properties of histone-1-anilinonaphthalene 8-sulfonate complexes in the presence of denaturant agents: application to the rapid staining of histones in urea and Triton-urea-polyacrylamide gels.
In the present report it is shown that histone bands in urea-acetic acid or Triton-urea-acetic acid-polyacrylamide gels can be stained with the fluorescent dye 1-anilinonaphthalene 8-sulfonate and visualized by transillumination of the gel with an uv-light source. The 1-anilinonaphthalene 8-sulfonate staining method described here for urea and Triton-urea gels is rapid (it can be completed in 90 min) and allows the detection of less than 1 micrograms of histone per band.
D000817:Anilino Naphthalenesulfonates; D000818:Animals; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006657:Histones / Q000032:analysis*; D011092:Polyethylene Glycols; D013050:Spectrometry, Fluorescence; D014508:Urea
D000817:Anilino Naphthalenesulfonates; D006657:Histones; D011092:Polyethylene Glycols; C027132:1-anilino-8-naphthalenesulfonate; D014508:Urea