Crystallization of calcium-calmodulin-trifluoperazine complex and an attempt at crystallizing calcium-free calmodulin.
Crystals suitable for X-ray structure analysis were obtained for CaM complexed with both calcium ions and a phenothiazine drug, trifluoperazine (TFP). The TFP/CaM binding ratio in crystals was experimentally determined to be nearly 1. An attempt at crystallizing calcium-free calmodulin (CaM) resulted in rigid but non-birefringent solids which exhibited no X-ray reflections.
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002118:Calcium / Q000302:isolation & purification; D002147:Calmodulin; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003460:Crystallization; D014268:Trifluoperazine; D014961:X-Ray Diffraction
D002147:Calmodulin; D014268:Trifluoperazine; D002118:Calcium