The half-lives of alkylating intermediates from diethylnitrosamine and N-nitrosopyrrolidine: a method for the measurement of metabolically generated reactive species.
The half-times of the alpha-hydroxylated intermediates formed during metabolism of diethylnitrosamine and N-nitrosopyrrolidine have been determined. The method for determining half-times involved in vitro enzymatic conversion of the nitrosamine to a hydroxylated intermediate followed by trapping of the alkylating species generated from the chemical decomposition of the intermediate as it flowed through a column containing bound nucleophile. Half-times of less than 1 min at pH 7.4, 37 degrees C, were calculated from the distribution of alkylation on the column and the known flow rate of metabolic intermediates through the column. The half-times may be short enough to significantly limit organ distribution.
D000478:Alkylation; D000818:Animals; D004052:Diethylnitrosamine / Q000378:metabolism*; D006207:Half-Life; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D009242:N-Nitrosopyrrolidine / Q000378:metabolism*; D009602:Nitrosamines / Q000378:metabolism*; D051381:Rats
D009602:Nitrosamines; D004052:Diethylnitrosamine; D009242:N-Nitrosopyrrolidine