Analysis of the expression of cloned HLA class I genes in mouse transfected L cells by quantitative flow cytometry.
By using a calibrated cell sorter and monoclonal antibodies conjugated to fluorochromes, a quantitative analysis of the levels of expression of HLA class I molecules at the surface of cloned murine L cells transfected with purified A3, B7, or CW3 genes was performed and compared with radioimmunoassay data. We selected clones of heterogeneous levels of HLA class I expression, which were shown to remain constant over a period of 4 mo in absence of HAT selection and not to be correlated to the DNA copy number of the corresponding integrated gene.
D000818:Animals; D000911:Antibodies, Monoclonal; D002469:Cell Separation / Q000379:methods; D004274:DNA, Recombinant; D005434:Flow Cytometry / Q000379:methods*; D005786:Gene Expression Regulation; D005796:Genes; D006680:HLA Antigens / Q000032:analysis / Q000235:genetics*; D006801:Humans; D051379:Mice; D009693:Nucleic Acid Hybridization; D014162:Transfection
D000911:Antibodies, Monoclonal; D004274:DNA, Recombinant; D006680:HLA Antigens