Trypanosoma brucei gambiense: growth in vitro of bloodstream forms inhibited by dichlorodiammineplatinum (II) compounds and hypolipidemic drugs.
Infectious bloodstream forms of Trypanosoma brucei gambiense were grown in microcultures of murine bone marrow cells in 96-well tissue culture plates. Limiting dilution studies showed that fewer than 10 cultured trypanosomes developed into populations of about 5 X 10(4) parasites per well in a week. Bloodstream parasites were reisolated with high efficiency from mice infected with cultured parasites; fewer than 10 bloodstream parasites successfully established a trypanosome population in a microculture. Both the cis and trans isomers of dichlorodiammineplatinum (II) (cisplatin and transplatin) and a hypolipidemic agent, Wy 14643, were found to have activity against T. b. gambiense growing in microcultures. The minimum concentration of drug necessary to completely eliminate parasites from microcultures was 4 microM for cisplatin, 40 microM for transplatin, and 500 microM for Wy 14643. A preformed complex of cisplatin and bovine serum albumin and another hypolipidemic agent, chlofibric acid, were inactive. This culture system should be useful for rapid screening of large numbers of compounds for trypanocidal activity.
D000818:Animals; D001853:Bone Marrow; D002478:Cells, Cultured; D002945:Cisplatin / Q000494:pharmacology*; D002995:Clofibric Acid / Q000494:pharmacology; D051379:Mice; D011743:Pyrimidines / Q000494:pharmacology*; D014347:Trypanosoma brucei gambiense / Q000187:drug effects* / Q000254:growth & development
D011743:Pyrimidines; C034697:transplatin; D002995:Clofibric Acid; C006253:pirinixic acid; D002945:Cisplatin