Comparison between inhibin from human and bovine ovarian follicular fluid using fast protein liquid chromatography.
Inhibin from human and bovine ovarian follicular fluid was purified 700-900-fold using affinity chromatography on immobilized Procion Red 3B, desalting on Sephadex G-25, ion-exchange chromatography on the fast protein liquid chromatography (FPLC) columns Mono Q and Mono P and chromatography on immobilized lectins. Isoelectric points for inhibin from human and bovine origin were between 5.1-5.7 and 4.75-5.25, respectively. Inhibin from both sources was retained by immobilized lectins, indicating its association with a glycoprotein. Overall recoveries of inhibin activity after these chromatographic procedures were approximately 1%.
D000818:Animals; D001826:Body Fluids / Q000032:analysis; D002417:Cattle; D002853:Chromatography, Liquid; D005260:Female; D006801:Humans; D007265:Inhibins / Q000032:analysis*; D006080:Ovarian Follicle / Q000032:analysis*; D013045:Species Specificity
D007265:Inhibins