Soluble IgG aggregates produced by heating remain stable on freeze-drying.
IgG aggregates produced by heating gamma globulin solutions were freeze-dried, kept at 4 degrees C and reconstituted up to 4 months later. By comparison with frozen (-20 degrees C) preparations, only minimal changes in biological reactivity and in physical integrity occurred during this period. These results demonstrate that freeze-dried preparations of heat-aggregated IgG are potentially useful as a reference reagent for the comparative evaluation and standardisation of immune complex assays.
D000936:Antigen-Antibody Complex; D002499:Centrifugation, Density Gradient; D005612:Freeze Drying; D006358:Hot Temperature; D006801:Humans; D007074:Immunoglobulin G
D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G