title: Nonprotein sulfhydryl compounds in canine gastric mucosa: effects of PGE2 and ethanol.
abstract: By use of an in vivo canine chambered stomach preparation in which the gastric mucosa was partitioned into two equal halves, the effect of topical 16,16-dimethyl PGE2 (DMPGE2) (1 microgram/ml of perfusate) and 8% and 40% ethanol on tissue levels of nonprotein sulfhydryl compounds was assessed. Both DMPGE2 and 8% ethanol significantly increased (P less than 0.005) mucosal levels of nonprotein sulfhydryls when compared with corresponding mucosa bathed with saline alone. In contrast, mucosa bathed with 40% ethanol showed significantly decreased levels. If mucosa was bathed with DMPGE2 or 8% ethanol prior to exposing the stomach to 40% ethanol, this depletion in sulfhydryl compounds was not observed. Since other experimental observations have shown that exogenously administered prostaglandins and mild irritants (such as low-dose alcohol) can prevent gastric mucosal damage by necrotizing agents (such as high-dose alcohol), our findings are consistent with the hypothesis that nonprotein sulfhydryls may play a role in mediating gastric mucosal protection.
mesh_terms: D015064:16,16-Dimethylprostaglandin E2 / Q000494:pharmacology*; D000818:Animals; D004285:Dogs; D000431:Ethanol / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D005260:Female; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D011459:Prostaglandins E, Synthetic / Q000494:pharmacology*; D013438:Sulfhydryl Compounds / Q000378:metabolism* / Q000502:physiology
chemical_list: D011459:Prostaglandins E, Synthetic; D013438:Sulfhydryl Compounds; D000431:Ethanol; D015064:16,16-Dimethylprostaglandin E2