title: Methods for chromatofocusing of cerebrospinal fluid and serum immunoglobulin G.
abstract: Chromatofocusing programs were designed for separations of submilligram amounts of normal and abnormal human IgG. The Pharmacia FPLC system, equipped with a Mono P column or a specially designed, small column was used for the separations. Normal IgG in paired cerebrospinal fluid and serum samples, paired samples from patients with intrathecal immunoglobulin G synthesis, as well as sera with IgG M components were examined. Abnormal immunoglobulin G components, especially those with pI values greater than ca. 7.0 pH units, were easily identified.
mesh_terms: D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000134:cerebrospinal fluid; D007525:Isoelectric Focusing; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007074:Immunoglobulin G