title: A functional plasminogen assay utilizing the potentiating effect of fibrinogen to correct for the overestimation of plasminogen in pathological plasma samples.
abstract: An overestimation of the plasminogen concentration occurs in patients with elevated levels of fibrin degradation products (fdp) and/or fibrinogen, when using assays based on the activation of plasminogen by streptokinase (Sk) followed by the hydrolysis of a synthetic chromogenic substrate. This source of error could be overcome by addition of fibrinogen in excess to the plasminogen assay thereby obtaining maximum stimulation of the Sk-plasminogen complex. The late degradation products of fibrinogen and fibrin, Dcate and Ddimer, respectively, had no potentiating effect on the Sk-plasminogen complex. The modified assay was designed as a manual method or for a centrifugal analyzer. The excellent reproducibility is illustrated by the coefficient of variation (CV) within series being 1.3% (n = 24) and from day to day being 1.9% (n = 20).
mesh_terms: D001322:Autoanalysis; D001780:Blood Coagulation Tests / Q000379:methods; D004211:Disseminated Intravascular Coagulation / Q000097:blood; D005189:False Positive Reactions; D005338:Fibrin Fibrinogen Degradation Products / Q000378:metabolism / Q000494:pharmacology; D005340:Fibrinogen / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D006868:Hydrolysis; D010958:Plasminogen / Q000032:analysis* / Q000378:metabolism; D013300:Streptokinase / Q000494:pharmacology
chemical_list: D005338:Fibrin Fibrinogen Degradation Products; C015214:fibrinogen D fragment; D005340:Fibrinogen; D010958:Plasminogen; D013300:Streptokinase