Aged human erythrocytes exhibit increased anion exchange.
Young and old erythrocytes show different rate constants of anion exchange as measured by 35SO4(2-) efflux at 37 degrees C. Results indicate that the rate constant for 35SO4(2-) efflux (SO2-4-Cl- exchange) from old cells is approximately 20% greater than from young less dense cells. The cell water volume of older cells is also decreased. Based on these results and previously reported decreases of cell membrane area in aged cells we conclude that anion exchange (35SO4(2-)) is increased in older, more dense human erythrocytes.
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D000328:Adult; D001693:Biological Transport, Active / Q000187:drug effects; D001834:Body Water / Q000378:metabolism; D002712:Chlorides / Q000097:blood*; D004905:Erythrocyte Aging; D005260:Female; D006531:HEPES; D006801:Humans; D008297:Male; D008433:Mathematics; D013431:Sulfates / Q000097:blood*
D002712:Chlorides; D013431:Sulfates; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D006531:HEPES