Evaluation of a purified Schistosoma japonicum glycoprotein egg antigen for the immunodiagnosis of infection in man.
A purified glycoprotein, glycoprotein-2, which was isolated from Schistosoma japonicum soluble egg antigen, was tested in an enzyme-linked immunoadsorbent assay for its potential as an immunodiagnostic reagent. Using empirically-derived criteria of endpoint titer, 39/40 sera from S. japonicum-infected individuals were scored correctly as positive in a blind test. Of these, 26/40 were from individuals who excreted fewer than 100 eggs per gram of stool. No cross reactions were observed with sera from uninfected individuals (0/20) or from individuals infected with S. mansoni (0/22), Trichinella spiralis (0/6) or Brugia malayi (0/4). Cross reactions were observed with 3/24 individuals infected with S. haematobium who excreted greater than 2,000 eggs per 10 ml urine. Glycoprotein-2 appears to satisfy the requisite criteria of specificity and sensitivity to warrant further evaluation.
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D051379:Mice; D010063:Ovum / Q000276:immunology; D012549:Schistosoma japonicum; D012552:Schistosomiasis / Q000175:diagnosis* / Q000276:immunology
D000947:Antigens, Helminth; D006023:Glycoproteins