A biochemical and immunological approach to the identification of H-Y antigenic proteins secreted from Daudi cells.
Conditioned culture medium from Daudi cells was used as a source of soluble H-Y antigen. Concentrated culture medium was labeled with 125I and then fractionated by gel filtration. Column fractions were assayed for the presence of H-Y antigen by urease-ELISA. H-Y antigen-containing fractions were then pooled and subjected to an improved immunoprecipitation protocol. Three predominant H-Y antigenic proteins were identified with estimated molecular weights of above 200,000, 50,000, and 20,000.
D001345:Autoradiography; D002051:Burkitt Lymphoma / Q000276:immunology* / Q000378:metabolism; D002460:Cell Line; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006182:H-Y Antigen / Q000032:analysis*; D006801:Humans; D007457:Iodine Radioisotopes
D003470:Culture Media; D006182:H-Y Antigen; D007457:Iodine Radioisotopes