title: Biochemical and genetic studies on mouse aldehyde dehydrogenases.
abstract: Aldehyde dehydrogenase (AHD) exists as isozymes which are differentially distributed among tissues and subcellular fractions of mouse tissues. Genetic variants for liver mitochondrial (AHD-1) and cytoplasmic (AHD-2) isozymes have been used to map the responsible loci (Ahd-1 and Ahd-2) on chromosomes 4 and 19 respectively. Evidence for a regulatory locus (Ahd-3r) controlling the inducibility of the mouse liver microsomal isozyme (AHD-3) has also been obtained. More recent studies have described genetic and biochemical evidence for three additional AHD isozymes: a stomach isozyme (AHD-4); another liver mitochondrial enzyme (AHD-5); and a testis isozyme (AHD-6). Genetic analyses have indicated that AHD-4 and AHD-6 are encoded by distinct but closely linked loci on the mouse genome (Ahd-4 and Ahd-6), which segregate independently of Ahd-1 and Ahd-2. Liver mitochondrial isozymes, AHD-1 and AHD-5, have been purified to homogeneity using affinity chromatography. The very high affinity of AHD-5 for acetaldehyde suggests that this enzyme is predominantly responsible for acetaldehyde oxidation in mouse liver mitochondria.
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000032:analysis* / Q000235:genetics; D000818:Animals; D002874:Chromosome Mapping; D003600:Cytosol / Q000201:enzymology; D007527:Isoenzymes / Q000032:analysis; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008930:Mitochondria, Liver / Q000201:enzymology; D010641:Phenotype; D013270:Stomach / Q000201:enzymology; D013737:Testis / Q000201:enzymology
chemical_list: D007527:Isoenzymes; D000444:Aldehyde Dehydrogenase