A comparison between effects of chronic ethanol consumption, ethanol withdrawal and fasting in ethanol-fed rats on the free cytosolic NADP+/NADPH ratio and NADPH-regenerating enzyme activities in the liver.
Effects of chronic ethanol consumption, withdrawal and fasting on the free cytosolic NADP+/NADPH ratio and NADPH-regenerating enzyme activities of rat liver were studied. Ethanol consumption was shown to decrease the NADP+/NADPH ratio in non-fasted rats, and both ethanol withdrawal and fasting in ethanol-fed animals appeared to increase the ratio to the normal or higher level. Any treatment of rats caused the complex interaction on hepatic NADPH-regenerating enzyme activities, none of the enzyme activity correlating with the free cytosolic NADP+/NADPH ratio. Relationship between free cytosolic NADP+/NADPH ratio and lipogenic capacity of withdrawn rat liver is discussed, and a hypothesis for development of the fatty liver is suggested.
D000437:Alcoholism / Q000378:metabolism*; D000818:Animals; D003600:Cytosol / Q000032:analysis*; D000431:Ethanol / Q000494:pharmacology; D005215:Fasting; D005227:Fatty Acids / Q000096:biosynthesis; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000032:analysis; D007521:Isocitrate Dehydrogenase; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D009243:NAD / Q000032:analysis*; D009249:NADP / Q000032:analysis* / Q000378:metabolism; D011773:Pyruvates / Q000032:analysis; D019289:Pyruvic Acid; D051381:Rats; D011919:Rats, Inbred Strains; D013375:Substance Withdrawal Syndrome / Q000378:metabolism*
D005227:Fatty Acids; D005958:Glucosephosphates; D011773:Pyruvates; D009243:NAD; D000431:Ethanol; D009249:NADP; D019298:Glucose-6-Phosphate; D019289:Pyruvic Acid; D007521:Isocitrate Dehydrogenase