Detection and enumeration of monocytes in human blood with peanut agglutinin.
Binding of peanut agglutinin (PNA) to normal human peripheral blood mononuclear cells was analyzed on a cell sorter, and compared to the binding of the monocyte specific monoclonal antibodies Mac-1 and Leu-M3. Each of the reagents labeled 9-11% of the mononuclear cells and similar binding patterns were observed. Of the PNA+ cells, 67% adhered to plastic petri dishes, whereas 76% of Mac-1+ cells were adherent. No competition for binding was observed between PNA and Mac-1 on the one hand, or PNA and Leu-M3 on the other. In double staining experiments, about 10% of the cells, comprising 80% of the monocytes, were PNA+ Leu-M3+. Our results show that PNA can serve for the identification and enumeration of monocytes in human peripheral blood.
D000911:Antibodies, Monoclonal / Q000276:immunology; D002469:Cell Separation; D006801:Humans; D037102:Lectins; D007958:Leukocyte Count / Q000379:methods*; D009000:Monocytes / Q000276:immunology; D019887:Peanut Agglutinin
D000911:Antibodies, Monoclonal; D037102:Lectins; D019887:Peanut Agglutinin