Hapten binding assay using guinea pig macrophages.
An assay for testing the binding of haptens to antigen-presenting cells was developed. The test consists of consecutive incubation of macrophages with the tested hapten, followed by incubation with a radioactively labeled standard. If the first hapten occupies a certain number of binding sites, the other one (radioactively labeled standard) reveals reduced binding. The binding assay in the present modification is fully suitable for measuring the binding of water-soluble haptens. It was found that from a panel of tested haptens, DNFB, beta-bromostyrol and phenylacetaldehyde exhibit strong binding.
D000079:Acetaldehyde / Q000031:analogs & derivatives / Q000378:metabolism; D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive; D002164:Camphor / Q000031:analogs & derivatives / Q000378:metabolism; D003374:Coumarins / Q000378:metabolism; D004139:Dinitrofluorobenzene / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology; D005260:Female; D006168:Guinea Pigs; D008264:Macrophages / Q000378:metabolism*; D011869:Radioligand Assay / Q000379:methods; D011946:Receptors, Antigen / Q000032:analysis*; D012459:Salicylates / Q000378:metabolism; D013343:Styrenes / Q000378:metabolism; D013501:Surface-Active Agents / Q000494:pharmacology
D000447:Aldehydes; D003374:Coumarins; D011946:Receptors, Antigen; D012459:Salicylates; D013343:Styrenes; D013501:Surface-Active Agents; C018238:beta-bromostyrene; D000431:Ethanol; D002164:Camphor; C038939:enzacamene; C030123:coumarin; C005556:propionaldehyde; D004139:Dinitrofluorobenzene; D000079:Acetaldehyde; C013192:phenylacetaldehyde