title: Isolation and characterization of nerve growth factor from Vipera lebetina (snake) venom.
abstract: Nerve growth factor from Vipera lebetina venom was purified by gel filtration and ion exchange chromatography steps. The NGF preparation obtained is a glycoprotein with weak arginine esterase activity. It hydrolyzes benzoylarginine ethyl ester (BAEE). Vipera lebetina NGF consists of multiple forms of protein with pI in the interval 9-10.5. All isoforms have identical molecular weights of 32,500.
mesh_terms: D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D002642:Chick Embryo; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005724:Ganglia / Q000187:drug effects; D066298:In Vitro Techniques; D007525:Isoelectric Focusing; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000302:isolation & purification* / Q000494:pharmacology; D012910:Snake Venoms / Q000302:isolation & purification; D012911:Snakes; D013045:Species Specificity; D014757:Viper Venoms / Q000302:isolation & purification*
chemical_list: D009414:Nerve Growth Factors; D012910:Snake Venoms; D014757:Viper Venoms; C019724:arginine esterase; D002265:Carboxylic Ester Hydrolases