Identification and isolation of the primary aggregation factor from the cell membrane of the sponge Geodia cydonium.
The primary aggregation factor (pAF) of sponge cells is a glycoprotein that is firmly associated with the cell membrane. Polyspecific antibodies (anti-GM) prepared from sera raised against membranes of cells from the siliceous sponge Geodia cydonium were found to inhibit initial aggregation of homologous cells. The inhibition of aggregation, caused by anti-GM was neutralized by pAF. The pAF had been successfully solubilized and enriched by affinity chromatography, gel filtration and density gradient centrifugation, if checked by polyacrylamide gel electrophoresis in the presence of urea. The Mr of the native pAF was approximately 40 000 as estimated by gel filtration; under denaturing conditions three protein species (Mr: 16 500, 15 500 and 13 500) were identified in the pAF preparation. The pAF was precipitable by Ca++ and did not cross-react with antisera against homologous purified secondary aggregation factor and lectin. It is mainly composed of protein (48.0%) and carbohydrate (50.2%). The isolated pAF restored the aggregation potency not only of factor-depleted Geodia cells but also of cells from other Demospongiae. However, the pAF displayed no aggregation enhancing effect on urea-treated cells from species belonging to the Calcispongiae or Hexactinellida. We hypothesize that in contrast to the secondary aggregation, the initial aggregation of Geodia cells is mediated by the one-component system, the bivalent and bifunctional pAF.
D000818:Animals; D015815:Cell Adhesion Molecules; D002449:Cell Aggregation / Q000187:drug effects; D002462:Cell Membrane / Q000032:analysis; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D008565:Membrane Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D011161:Porifera / Q000032:analysis*; D013045:Species Specificity
D015815:Cell Adhesion Molecules; D006023:Glycoproteins; D008565:Membrane Proteins; C013065:cell aggregation factors