Effects of spinal fusion on the proteoglycans of the canine intervertebral disc.
Posterior lumbar two-level spinal fusion was undertaken in 10 mature beagles. The animals were sacrificed 6 and 12 months later. Two months before sacrifice control and experimental animals received intravenously Na2(35)SO4 (1 mCi/kg). Discs encompassed by the fusion and those adjacent to it were dissected into the nucleus pulposus and annulus fibrosus (AF), which were analysed separately. Proteoglycans (PGs) were extracted with 4.0 M guanidine HCl and purified by CsCl density gradient ultracentrifugation. The hydrodynamic size and ability of the PG subunits to aggregate in the presence of hyaluronic acid were investigated by Sepharose CL-2B chromatography. The PG subunits were analysed for their galactosamine (galN), glucosamine (glcN), hexuronic acid, and protein content or were subjected to digestion with papain or chondroitin-ABC-lyase to establish the size of the chondroitin (CS) and keratan (KS) sulphate chains and the KS-PG core protein complex. Decreased ability to aggregate of PGs isolated from discs 6 and 12 months after surgery was demonstrated. While their hydrodynamic size after 6 months was generally the same or smaller than those in control tissues, the PG population present after 12 months was larger, particularly in the AF. Analysis of PG subunits from fusion discs afforded galN/glcN, galN/protein, and hexuronic acid/protein ratios that were compatible with the presence in these tissues of PGs in which the proportion of CS attached to core protein was greater than in control tissues. These studies provide the first experimental evidence that a metabolic response of discs in a fused segment may be accompanied by the biosynthesis of a new PG population whose structure is similar to that present in immature tissues.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002807:Chondroitin / Q000032:analysis; D002847:Chromatography, Agarose; D002850:Chromatography, Gel; D004285:Dogs; D005260:Female; D006603:Hexuronic Acids; D007403:Intervertebral Disc / Q000378:metabolism*; D007632:Keratan Sulfate / Q000032:analysis; D008159:Lumbar Vertebrae; D046911:Macromolecular Substances; D008297:Male; D008970:Molecular Weight; D011509:Proteoglycans / Q000302:isolation & purification / Q000378:metabolism*; D013123:Spinal Fusion
D006603:Hexuronic Acids; D046911:Macromolecular Substances; D011509:Proteoglycans; D002807:Chondroitin; D007632:Keratan Sulfate