The effect of methotrexate on in vitro immunoglobulin production.
The effect of Methotrexate (MTX) on pokeweed mitogen (PWM) induced immunoglobulin (Ig) production in vitro was studied over a range of 10(-4) - 10(-12)M MTX, using an enzyme-linked-immunosorbent assay (ELISA). MTX, at a concentration of 5 X 10(-9)M profoundly decreased all classes of Ig production, IgM greater than IgG greater than IgA. Plasma cell numbers, identified using polyclonal antihuman immunoglobulin, demonstrated a similar sensitivity to MTX. There was a paradoxical increase in IgM and IgG production at 5 X 10(-11)M MTX. Clinical implications of these findings are discussed.
D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008297:Male; D008727:Methotrexate / Q000494:pharmacology*; D010950:Plasma Cells / Q000187:drug effects; D011043:Pokeweed Mitogens / Q000494:pharmacology
D007136:Immunoglobulins; D011043:Pokeweed Mitogens; D008727:Methotrexate