[Electron microscopic-cytochemical study of succinate dehydrogenase activity of nerve tissue cells of human embryos in vitro].
The ultrastructural localization of succinate dehydrogenase (SDH) activity was revealed in astrocyte-like cells of 26 days old nervous tissue organotypic cultures. The Kerpel-Fronius and Hajos (1968) technique was employed using potassium ferricyanide as an artificial acceptor of electrons. The localization of the reaction product was seen more often in cells with long, thread-like mitochondria. The appearance of such organelles with a large membrane length and high enzymatic activity is suggested to reflect one of the mechanisms of adaptation of nervous tissue cells providing the adequate level of the energy supply under in vitro conditions.
D001253:Astrocytes / Q000201:enzymology / Q000648:ultrastructure; D001921:Brain / Q000196:embryology; D046508:Culture Techniques; D006651:Histocytochemistry; D006801:Humans; D008854:Microscopy, Electron; D009417:Nerve Tissue / Q000201:enzymology / Q000648:ultrastructure*; D013385:Succinate Dehydrogenase / Q000378:metabolism*
D013385:Succinate Dehydrogenase
Elektronno-tsitokhimicheskoe issledovanie aktivnosti suktsinatdegidrogenazy v kletkakh nervnoĭ tkani émbrionov cheloveka in vitro.