A rapid method for the purification of cytoplasmic paramyxoviral nucleocapsids.
A rapid method for the purification of cytoplasmic paramyxoviral nucleocapsids using a tabletop ultracentrifuge is described. The method yields materials equivalent in purity to materials purified by previously published procedures but requires only a fraction of the time (65 min vs 7-41.5 h). When used in conjunction with modern hypersensitive analytical techniques, the procedure provides equivalent or greater amounts of nucleocapsid for subsequent analysis or study.
D000650:Amnion; D002213:Capsid / Q000302:isolation & purification*; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003593:Cytoplasm / Q000382:microbiology; D006801:Humans; D008459:Measles virus / Q000032:analysis*