Reconstitution of an active surface T3/T-cell antigen receptor by DNA transfer.
We have introduced a full-length complementary DNA of the T-cell antigen receptor beta-chain into a mutant human T-cell line that lacked a complete beta-chain messenger RNA, had a diminished level of alpha-chain transcript and did not express surface T3- or antigen receptor. Expression of the transfected beta-chain led to a normal level of alpha-chain transcript and a structurally and functionally active T3 T-cell antigen receptor complex on the cell surface.
D002460:Cell Line; D004247:DNA / Q000235:genetics*; D006801:Humans; D009154:Mutation; D011506:Proteins / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D011948:Receptors, Antigen, T-Cell / Q000032:analysis* / Q000235:genetics; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D011506:Proteins; D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell; D004247:DNA