Improved methodology for the simultaneous detection of the trichothecene mycotoxins deoxynivalenol and nivalenol in cereals.
A simple and sensitive method has been developed for the analysis of two trichothecene mycotoxins (deoxynivalenol and nivalenol) in cereals. These toxins were extracted with acetonitrile/water (3:1), defatted with n-hexane and purified by a two-step chromatographic procedure using Florisil and Sep-pak columns. The amounts of deoxynivalenol (DON) and nivalenol (NIV) in the column eluates were quantitated by gas chromatography with electron capture detector and gas chromatography-mass spectrometry (selected ion monitoring). The limits of detection of the method were 2.0 micrograms/kg for DON and NIV with recoveries of the toxins spiked into polished rice, wheat and corn at 300 micrograms/kg averaging 87% and 86% respectively.
D002849:Chromatography, Gas; D002523:Edible Grain / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D007202:Indicators and Reagents; D009183:Mycotoxins / Q000032:analysis*; D012275:Oryza / Q000032:analysis; D012717:Sesquiterpenes / Q000032:analysis*; D014255:Trichothecenes / Q000032:analysis*; D014908:Triticum / Q000032:analysis; D003313:Zea mays / Q000032:analysis
D007202:Indicators and Reagents; D009183:Mycotoxins; D012717:Sesquiterpenes; D014255:Trichothecenes; C038405:nivalenol; C007262:deoxynivalenol