Characterization of the chymotryptic core of the adenovirus DNA-binding protein.
A fragment of the DNA-binding protein of adenovirus type 5 has been obtained by controlled chymotryptic digestion of the entire molecule. Partial sequence determination indicates that the fragment consists of amino acids 174-525. The fragment is biologically active as measured by its ability to substitute for the entire molecule in a reconstituted DNA replication system. Crystals have been obtained that show diffraction to 2 A.
D000256:Adenoviridae / Q000032:analysis*; D000595:Amino Acid Sequence; D002918:Chymotrypsin; D003460:Crystallization; D003461:Crystallography; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis; D014764:Viral Proteins / Q000032:analysis*
D004268:DNA-Binding Proteins; D010446:Peptide Fragments; D014764:Viral Proteins; D002918:Chymotrypsin