Self-renewal capacity of murine hemopoietic stem cells under internal contamination with 239Pu and 241Am.
The self-renewal capacity of murine pluripotent hemopoietic stem cells (CFU-S) of vertebral bone marrow was studied under conditions of short-term and long-term internal contamination with 239Pu or 241Am in female mice. Measurement of the CFU-S self-renewal capacity was carried out using double transplantation assay. To evaluate the production of differentiated progeny of stem cells average erythroblast numbers/visible spleen colony and 59Fe-uptake/colony were computed. The marrow cellularity/vertebra and the number of CFU-S/vertebra were decreased and affected more by 239Pu than by 241Am. The production of erythroblasts per a single CFU-S and the 59Fe-uptake/colony were reduced, similarly the numbers of secondary spleen colonies and of secondary CFU-S in primary colonies. The above changes resulting from impaired functions of surviving CFU-S were more serious with 241Am than with 239Pu. The biological effects of plutonium and americium appeared independent of the phase of contamination.
D000512:Alpha Particles; D000576:Americium; D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000528:radiation effects; D002455:Cell Division / Q000528:radiation effects; D003114:Colony-Forming Units Assay; D004900:Erythroblasts / Q000378:metabolism / Q000528:radiation effects; D005260:Female; D006412:Hematopoietic Stem Cells / Q000528:radiation effects*; D007501:Iron / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D011005:Plutonium; D011828:Radiation Chimera
D011005:Plutonium; D007501:Iron; D000576:Americium