Effect of povidone, povidone-iodine, and iodide on locomotion (in vitro) of neutrophils from people, rats, dogs, and rabbits.
The effect of the antiseptic povidone-iodine (P-I) on neutrophil locomotion was tested in vitro. Neutrophil migration into Micropore filters was significantly stimulated by P-I at concentrations between 0.03% and 0.005%. At higher concentrations (greater than or equal to 0.05%), a dose-dependent inhibition of neutrophil migration could be observed which was due to cytotoxic effects of P-I as shown by pyknosis and cell lysis. To analyze these effects, 2 components of P-I (namely, povidone and iodide) were tested separately. In these tests, povidone induced a pronounced stimulation of neutrophil migration at concentrations similar to the stimulatory concentrations of P-I. Inhibition of neutrophil migration or cytotoxic effects of povidone was not observed, even when tested at high concentrations (5.0%). Iodide (as NaI or KI) was cytotoxic and strongly inhibited neutrophil migration when it was tested at concentrations greater than that likely to be present in the inhibitory concentrations of P-I. Stimulatory effects of iodide on neutrophil migration could not be observed when tested over a wide range of noncytotoxic concentrations. Free iodine was not tested, but was considered to be the toxic component by exclusion. The patterns of response were similar for neutrophils from the 4 species tested. Migration of rabbit neutrophils, however, was inconsistently and weakly stimulated by P-I or povidone. These data indicate that the widely used antiseptic P-I, depending on its concentration, can either stimulate or inhibit neutrophil migration.
D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D004285:Dogs; D006801:Humans; D007454:Iodides / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D011205:Povidone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D011206:Povidone-Iodine / Q000494:pharmacology*; D011817:Rabbits; D051381:Rats; D013045:Species Specificity
D007454:Iodides; D011206:Povidone-Iodine; D011205:Povidone