Cloning of a gene from Streptomyces species complementing argG mutations.
A gene that complements argG mutations was cloned from Streptomyces coelicolor and Streptomyces lividans by using pIJ702 as a vector. The recombinant plasmid pMCP25 complemented argG mutations of S. lividans and S. coelicolor. The inserted DNA of pMCP25 was able to hybridize with argG+ strains of S. lividans, S. coelicolor, S. lavendulae and S. alboniger but not with argG mutants of these strains.
D002872:Chromosome Deletion; D003001:Cloning, Molecular; D005784:Gene Amplification; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D009154:Mutation; D010641:Phenotype; D010957:Plasmids; D013302:Streptomyces / Q000235:genetics*; D014169:Transformation, Bacterial