Primary structure of two major glycans of bovine fibrinogen.
After pronase digestion of bovine fibrinogen, the asparagine-linked glycans were released from the resulting glycopeptides by hydrazinolysis, and subsequently re-N-acetylated. Two sialylated glycans were isolated by ion-exchange chromatography. Their primary structure has been determined by methylation analysis and 360-MHz 1H-NMR spectroscopy. The structures proposed to be present in the native glycoprotein are as follows: (formula: see text).
D000818:Animals; D002240:Carbohydrate Sequence; D002417:Cattle; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D005340:Fibrinogen; D006020:Glycopeptides / Q000032:analysis; D006834:Hydrazines; D006868:Hydrolysis; D019158:N-Acetylneuraminic Acid; D009844:Oligosaccharides / Q000032:analysis; D010446:Peptide Fragments / Q000032:analysis; D011134:Polysaccharides / Q000032:analysis; D012794:Sialic Acids / Q000032:analysis
D006020:Glycopeptides; D006834:Hydrazines; D009844:Oligosaccharides; D010446:Peptide Fragments; D011134:Polysaccharides; D012794:Sialic Acids; C016735:sialooligosaccharides; C029424:hydrazine; D005340:Fibrinogen; D019158:N-Acetylneuraminic Acid