Liquid chromatographic determination of nicarbazin in feed.
A liquid chromatographic method was developed for the determination of nicarbazin (4,4'-dinitrocarbanilide.2-hydroxy-4,6-dimethylpyrimidine) in chicken feed. Ground feed was extracted with hot dimethylformamide, filtered, and then cleaned up on an alumina column. The nicarbazin was eluted from the column with ethanol and quantitated using a reverse phase C-18 column, with a methanol-water mobile phase and ultraviolet detection at 344 nm. Recoveries at a typical use level of 100 micrograms/g feed averaged 98% with a standard deviation of 3%. Samples fortified at levels as low as 0.1 micrograms/g were analyzed with 92% recovery. The detection limit is 1 ng, and the response is linear between 4 and 1000 ng. Feed additives in combination with nicarbazin do not interfere with recovery.
D000821:Animal Feed / Q000032:analysis*; D002224:Carbanilides / Q000032:analysis*; D002853:Chromatography, Liquid; D009528:Nicarbazin / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D002224:Carbanilides; D009528:Nicarbazin