The effects of dimethyl sulfoxide on the osmotic properties of a rat megakaryocytopoietic cell line.
Dimethyl sulfoxide (DMSO) at 0.6 M alters the osmotic state of intracellular water in a line of rat megakaryocytopoietic cells. The response is a function of temperature. Maximal structuring of water occurs at 24.7 degrees C, while disorganization occurs at 30 and 37 degrees C. Little effect occurs at 3 and at 18 degrees C. Parallel measurements of membrane permeability to water indicate that DMSO inhibits the osmotic exit of water at all temperatures. Maximal inhibition occurs at 24.7 degrees C, the same temperature at which maximal organization of water occurs. These findings emphasize the possible role which water may play in explaining the diverse functions of DMSO as cryogenic substance, promoter of cell fusion, and stimulator of cell differentiation.
D000818:Animals; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D066298:In Vitro Techniques; D008533:Megakaryocytes / Q000187:drug effects* / Q000502:physiology; D009997:Osmotic Pressure; D051381:Rats; D014867:Water / Q000502:physiology
D014867:Water; D004121:Dimethyl Sulfoxide