Flow-through pH-stat method for lipase activity.
A new method for lipase activity which combines the simplicity and rapidity of continuous pH-stat methods with the flexibility in choice of conditions (pH, temperature, etc.) of manual methods is described. The lipase-catalyzed hydrolysis of olive oil and titration of fatty acid products can be carried out separately in two small continuously stirred reactors in series. The response is linear with enzyme dilution.
D001322:Autoanalysis / Q000295:instrumentation; D002175:Candida / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000032:analysis*
D008049:Lipase