title: Improvement of sporulation in the yeast Yarrowia lipolytica.
abstract: Strains of Yarrowia lipolytica forming exclusively spherical ascospores were developed through inbreeding. These strains are more suitable for micromanipulation than other inbred strains forming helm-shaped ascospores. External factors affecting sporulation frequency and tetrad formation in this yeast were investigated. Optimal formation of complete tetrads occurred at a narrow range of pH values around 6.0. Citrate was found to stimulate sporulation strongly. A synthetic medium containing citrate was developed to obtain standard conditions for maximum sporulation.
mesh_terms: D001203:Ascomycota / Q000235:genetics / Q000502:physiology*; D002951:Citrates / Q000378:metabolism; D019343:Citric Acid; D003227:Conjugation, Genetic; D003433:Crosses, Genetic; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D010641:Phenotype; D013172:Spores, Fungal / Q000235:genetics / Q000502:physiology / Q000648:ultrastructure
chemical_list: D002951:Citrates; D003470:Culture Media; D019343:Citric Acid