Chromatographic techniques for the separation of a thrombocytopoiesis-stimulating factor from aplastic rats.
Thrombocytopoietin seems to be only partially responsible for the regulation of platelet production. We determined precisely the differences between a second thrombocytopoiesis-stimulating factor (TSF2), and thrombocytopoietin (TSF1) and erythropoietin (Epo). Fractionation was carried out, first on a DEAE-cellulose phosphate column and then on a Sephadex G-75 column. Thrombocytopoietic activity in the various fractions was assessed using 75Se-methionine platelet incorporation into normal recipients. Epo concentrations were determined using a radioimmunoassay. We showed that the apparent molecular weight of TSF2 is 14,000 daltons. It differs from TSF1 (48,000 daltons) and from Epo (39,000 daltons). For doses of 8-12 mU Epo/rat, found in whole serum injected, no effect on thrombocytopoiesis was found. On the contrary, a significant effect (p less than 0.01) was found when the same quantities of Epo present in the Sephadex G-75 F4' fraction were injected (2-10 mU/rat). TSF2 can be separated from TSF1 and Epo, using biochemical techniques.
D000741:Anemia, Aplastic / Q000097:blood* / Q000139:chemically induced; D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004921:Erythropoietin / Q000097:blood; D006023:Glycoproteins / Q000032:analysis*; D006918:Hydroxyurea; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D013926:Thrombopoietin / Q000032:analysis*
D006023:Glycoproteins; D004921:Erythropoietin; D013926:Thrombopoietin; D006918:Hydroxyurea