title: Deposition of type X collagen in the cartilage extracellular matrix.
abstract: In cultured chick embryo chondrocytes, type X collagen is preferentially deposited in the extracellular matrix, the ratio between type II and type X collagen being about 5 times higher in the culture medium than in the cell layer. When the newly synthesized collagens deposited in slices from the epiphyseal cartilage of 17-day-old embryo tibiae were isolated, type X collagen was always the major species. In agreement with this result the mRNA for type X collagen was the predominant mRNA species purified from the same tissue. When the total collagen (unlabeled) deposited in the epiphyseal cartilage was analyzed, it was observed that type X collagen represented only 1/15 of the type II collagen recovered in the same preparation. The possible explanations for these differences are discussed.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003094:Collagen / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003470:Culture Media / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005109:Extracellular Matrix / Q000378:metabolism*; D006132:Growth Plate / Q000032:analysis*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D003470:Culture Media; D012333:RNA, Messenger; D003094:Collagen