Stimulation of division in mouse 3T3 cells by coculture with embryonic chick limb tissue.
Two regions of the chick limb bud--the apical ectodermal ridge and the zone of polarizing activity--have been shown to influence cell division and pattern formation during normal development and following surgical manipulation. In this study, using a simple coculture system, together with autoradiography, we have shown that these morphogenetically active regions of the limb bud can stimulate quiescent 3T3 cells to initiate DNA synthesis to a significantly greater degree than comparable but morphogenetically inactive regions of the limb bud.
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D002642:Chick Embryo; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D004475:Ectoderm / Q000166:cytology; D005528:Foot / Q000196:embryology; D051379:Mice; D009024:Morphogenesis; D014034:Toes / Q000196:embryology
D003470:Culture Media; D004247:DNA