Effect of riboflavin-butyrate on cardiac glutathione reductase affected by adriamycin.
Male Wistar rats received intraperitoneal injections of adriamycin (4 mg/kg body weight/day) and/or riboflavin-butyrate (20 mg/kg body weight/day) for 6 consecutive days. Cardiac mitochondria were then prepared for our present experiment. The combined use of riboflavin-butyrate with adriamycin was evaluated for reduction of lipid peroxide formation in rat cardiac mitochondria. In order to find the mechanism of the effect of riboflavin-butyrate, the glutathione peroxidase-glutathione reductase system was examined. Adriamycin reduced the glutathione reductase activity in rat cardiac mitochondria, but did not affect the glutathione peroxidase activity. The mitochondrial content of flavin adenine dinucleotide, a prosthetic group of glutathione reductase, was greatly reduced and apoprotein of glutathione reductase also decreased. The administration of riboflavin-butyrate with adriamycin increased flavin adenine dinucleotide and glutathione reductase activity. These results suggest that exogenous administration of riboflavin-butyrate is capable of reducing lipid peroxide by both enzymatic detoxification through glutathione reductase and non-enzymatic detoxification due to direct reaction with lipid peroxide.
D000818:Animals; D004317:Doxorubicin / Q000494:pharmacology*; D005486:Flavin Mononucleotide / Q000378:metabolism; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism*; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D012256:Riboflavin / Q000031:analogs & derivatives* / Q000494:pharmacology
D008054:Lipid Peroxides; D005182:Flavin-Adenine Dinucleotide; C024261:riboflavin-5'-monobutyrate; D005486:Flavin Mononucleotide; D004317:Doxorubicin; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D012256:Riboflavin