Immunoturbidimetry of serum C-reactive protein in low concentration of polyethylene glycol.
A simple, rapid immunoturbidimetric assay for determination of serum C-reactive protein is presented. In order to obtain constant sample blank values within a few minutes we found it necessary to use a low concentration (10 g/L) of polyethylene glycol 6000 in contrast to the commonly used 40 g/L PEG solution. The sensitivity of the assay presented is 7 mg/L; within-run and between-run CVs ranged respectively from 1.4% to 2.4% and from 2.1% to 5.5%. Results obtained with the proposed method correlate well with radial immunodiffusion.
D002097:C-Reactive Protein / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D011092:Polyethylene Glycols; D011786:Quality Control
D011092:Polyethylene Glycols; D002097:C-Reactive Protein