Immunofluorescent detection of anti-cytoskeleton antibodies using vinblastine-treated mononuclear cells.
A reliable and reproducible immunofluorescence method is described for the detection of anti-cytoskeleton antibodies in human sera, based on the use of vinblastine-treated peripheral blood mononuclear cells as substrate. Three immunofluorescence patterns associated with antibodies to microfilaments, intermediate filaments and microtubules are readily identified.
D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D003598:Cytoskeletal Proteins / Q000276:immunology*; D003599:Cytoskeleton / Q000276:immunology; D005347:Fibroblasts / Q000648:ultrastructure; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000648:ultrastructure; D008870:Microtubules / Q000276:immunology; D009000:Monocytes / Q000187:drug effects / Q000648:ultrastructure; D009130:Muscle, Smooth / Q000276:immunology; D014747:Vinblastine
D000906:Antibodies; D003598:Cytoskeletal Proteins; D014747:Vinblastine