[Colony-forming ability of precursor cells of granulomonocytopoiesis in an agar bilayer system in healthy subjects].
It is necessary to know growth characteristics of normal hemopoietic cells in order to estimate their clonogenic properties at different diseases of the blood system. Twelve healthy persons at the age of 27-49 years have been examined. Cellular aggregates have been counted on the 7th day. The aggregates containing more than 20 cells are considered as colonies, the aggregates containing 3-20 cells--as clusteres. The number of the aggregates is recounted as 1.10(5) nuclear cells. Among the colonies the overwhelming majority belong to neutrophilic ones, among the clusters this predominance is not so clearly manifested. The cells become mature up to the stab and segmental neutrophils and monocytes. Among the clusters, besides the neutrophilic aggregates, there is an essential part of the macrophagal ones.
D000328:Adult; D000362:Agar; D003114:Colony-Forming Units Assay; D003470:Culture Media; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology*; D009504:Neutrophils / Q000166:cytology*
D003470:Culture Media; D000362:Agar
Kolonieobrazuiushchaia sposobnost' kletok-predshestvennikov granulotsitomonopoéza v dvukhsloĭnoĭ agarovoĭ sisteme u zdorovykh liudeĭ.