Micropreparative isotachophoretic electrodesorption of monoclonal antibodies from an affinity adsorbent.
The principle of isotachophoretic desorption of proteins from an affinity sorbent was used for the preparative isolation of monoclonal antibodies against transferrin, under a cationic regimen. Electrodesorption was carried out on an apparatus of our own construction with a photometric detector and a detector of potential gradient. The apparatus was provided with a sorption element filled with an exchangeable affinity active sorbent. The construction of the apparatus and the procedure suitable for the isolation of the antibody of the IgG type are described. The possibility of repeating the sorption-desorption cycle at least sixteen times was demonstrated, with relatively good yields of the protein.
D000327:Adsorption; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D004563:Electrochemistry; D004573:Electrolytes; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D008832:Microchemistry; D011506:Proteins / Q000302:isolation & purification; D013056:Spectrophotometry, Ultraviolet
D000911:Antibodies, Monoclonal; D004573:Electrolytes; D011506:Proteins