Determination of molecular hydrogen in investigations of the susceptibility of Enterobacteriaceae to ampicillin.
One hundred and five enterobacterial isolates from urinary tract infections were examined for ampicillin-susceptibility by measurement of molecular hydrogen production with a palladium metal oxide semi-conductor (Pd-MOS sensor). Eighty-one per cent of hydrogen producing strains could be placed in the same susceptibility groups as recorded by disc-diffusion tests. However, it was harder to separate susceptible from intermediate types than to determine resistance. In all but five strains the hydrogen production allowed determination of viability within 5 h.
D000667:Ampicillin / Q000494:pharmacology*; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000378:metabolism; D006859:Hydrogen / Q000032:analysis*; D008826:Microbial Sensitivity Tests
D000667:Ampicillin; D006859:Hydrogen