Plasma vitellogenin in landlocked Atlantic salmon (Salmo salar Ouananiche): isolation, homologous radioimmunoassay and immunological cross-reactivity with vitellogenin from other teleosts.
Vitellogenin was isolated by affinity chromatography and gel filtration from landlocked Atlantic salmon plasma. Vitellogenin was labelled with iodine-131 using iodogen and an homologous radioimmunoassay was developed. There was poor immunological cross-reactivity with vitellogenin or plasma from other teleosts. Parallelism of the vitellogenin standard to the displacement by plasma of vitellogenic salmon allowed the assay to be used to evaluate the seasonal concentration profile of vitellogenin in female adult salmon. Extracts of liver or ovary from female Atlantic salmon also yielded displacements parallel to the vitellogenin standard in the assay.
D000818:Animals; D002846:Chromatography, Affinity; D003429:Cross Reactions; D004589:Electrophoresis, Disc; D005260:Female; D005399:Fishes / Q000097:blood*; D005618:Fresh Water; D008074:Lipoproteins / Q000097:blood*; D010053:Ovary / Q000032:analysis; D011863:Radioimmunoassay; D012474:Salmon / Q000097:blood*; D013045:Species Specificity; D014819:Vitellogenins / Q000032:analysis / Q000097:blood*
D008074:Lipoproteins; D014819:Vitellogenins