Determination of amino acids by separation of their ion pairs with dodecyl sulphate.
The ion pairs of amino acids with dodecyl sulphate were separated on a reversed-phase column (Beckman Ultrasphere I.P.) using a sequence of eluents that are prepared by mixing 0.2 M phosphoric acid (containing 10 mM sodium dodecyl sulphate), 0.2 M sodium acetate (pH 4.50; containing 10 mM sodium dodecyl sulphate) and methanol. the amino acids commonly occurring in tissues, except taurine and related weakly basic amino acids, can be analysed at a rate of 95 min per sample at a sensitivity of less than 50 pmol per amino acid. Elution modes for specific amino acids (alpha-difluoromethylornithine, gamma-vinyl-4-aminobutyric acid, 4-aminobutyric acid, putreanine) and non-essential amino acids that allow higher separation rates are reported. The method is suitable for fully automated routine amino acid analysis.
D000596:Amino Acids / Q000032:analysis* / Q000302:isolation & purification; D000599:Amino Acids, Diamino / Q000032:analysis; D000614:Aminocaproates / Q000032:analysis; D000818:Animals; D001923:Brain Chemistry; D006863:Hydrogen-Ion Concentration; D007474:Ion Exchange; D008099:Liver / Q000032:analysis; D008623:Mercaptoethanol; D051379:Mice; D010455:Peptides / Q000032:analysis; D012967:Sodium Dodecyl Sulfate; D012997:Solvents; D014020:Tissue Extracts / Q000032:analysis; D020888:Vigabatrin; D005680:gamma-Aminobutyric Acid / Q000032:analysis; D009764:o-Phthalaldehyde
D000596:Amino Acids; D000599:Amino Acids, Diamino; D000614:Aminocaproates; D010455:Peptides; D012997:Solvents; D014020:Tissue Extracts; C000064:putreanine; D012967:Sodium Dodecyl Sulfate; D005680:gamma-Aminobutyric Acid; D008623:Mercaptoethanol; D009764:o-Phthalaldehyde; D020888:Vigabatrin