Immunological characterization of human acid phosphatase gene products.
The immunological cross-reactivity of heterogeneous acid phosphatase isozymes from different human tissues has been studied using monospecific antisera prepared against four homogeneous acid phosphatases. The enzyme characterized as tartrate-inhibitable, prostatic acid phosphatase is also found to be present in leukocytes, kidney, spleen, and placenta. The tartrate-inhibitable (liver) lysosomal enzyme is also found in kidney, fibroblasts, brain, placenta, and spleen, but it is not detectable in erythrocytes and prostate. In several tissues, 10-20% of the tartrate-inhibitable enzyme is not precipitated by any of the antisera used; an exceptionally high amount (54%) of such an enzyme is present in human brain. Antiserum against a low molecular weight tartrate-resistant liver enzyme (14 kDa) does not crossreact with the erythrocyte enzyme. (10-20 kDa). All other tissues except placenta, prostate, and fibroblast cells show a cross-reactivity with the 14-kDa acid phosphatase antiserum. Thus, the low molecular weight human liver acid phosphatase is distinct from the erythrocyte enzyme, and there are also at least three different tartrate-inhibitable acid phosphatases in human tissues. Chromosomal assignments have been made for only two of the (at least) five acid phosphatases that are present in adult human tissues.
D000135:Acid Phosphatase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology*; D001773:Blood Cells / Q000201:enzymology; D001921:Brain / Q000201:enzymology; D003429:Cross Reactions; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000276:immunology*; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D010920:Placenta / Q000201:enzymology; D011467:Prostate / Q000201:enzymology; D013154:Spleen / Q000201:enzymology; D013644:Tartrates / Q000494:pharmacology
D007527:Isoenzymes; D013644:Tartrates; D000135:Acid Phosphatase; C029768:tartaric acid