title: The FRAME interlaboratory programme on in vitro cytotoxicology.
abstract: FRAME (the Fund for the Replacement of Animals in Medical Experiments) has established a research programme in collaboration with four research centres and a number of industrial companies, to determine whether cell cultures can reliably be used to replace live animal procedures in routine tests on the relative acute toxicities of chemicals. Following the development of the test protocol, initial trials were carried out to establish intralaboratory and interlaboratory reproducibility and the criteria for choice of cell types, end-points and metabolizing systems. A set of coded chemicals is being collected for use in blind trials and summaries of existing knowledge of the in vivo toxicity of these compounds are being produced. More recently, FRAME has established an international validation scheme for in vitro alternative methods.
mesh_terms: D000826:Animal Testing Alternatives; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D007928:Lethal Dose 50; D011506:Proteins / Q000032:analysis; D014116:Toxicology / Q000379:methods*
chemical_list: D011506:Proteins