A simplified method for isocratic HPLC analysis of polyamines.
A simple technique is described for the separation and analysis of polyamines in tissues and body fluids, utilizing precolumn clean-up on disposable CM-cellulose columns, followed by an automatable HPLC procedure. Complete separation and analysis takes 12-15 min per sample with sensitivity in the pmole range.
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange; D011073:Polyamines / Q000032:analysis*; D011700:Putrescine / Q000032:analysis; D051381:Rats; D013095:Spermidine / Q000032:analysis; D013096:Spermine / Q000032:analysis; D014018:Tissue Distribution
D011073:Polyamines; D013096:Spermine; D013095:Spermidine; D011700:Putrescine