Solubilization and partial purification of a cell surface component of Leishmania braziliensis.
A simple procedure that allowed the extraction and partial purification of a component of 65,000 mol.wt. from the surface of Leishmania braziliensis promastigotes is described. Iodinated cell surface membrane fractions were solubilized using Triton X-100 followed by Nonidet P-40. The macromolecular components were then freed of the detergents by passage of the extracts through a column of DE52 cellulose. The component of 65,000 mol.wt. was eluted from the column with 1 m NaCl. This component in whole parasites was immunoprecipitated by sera from patients with cutaneous, mucocutaneous, and diffuse cutaneous leishmaniasis and kala-azar. None of the major surface determinants reacted with sera from normal individuals with antibovine rabbit serum. The relevance and the possible applications on the immunoprophylaxis of the disease are briefly discussed.
D000818:Animals; D000953:Antigens, Protozoan / Q000302:isolation & purification*; D000954:Antigens, Surface / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D007891:Leishmania / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology / Q000302:isolation & purification*; D008970:Molecular Weight; D011233:Precipitin Tests; D011863:Radioimmunoassay; D012995:Solubility; D013010:Sonication
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D008565:Membrane Proteins