Effects of marrow storage at 4 degrees C on the subsequent generation of long-term marrow cultures.
The present study was undertaken to examine the effect of marrow preservation at 4 degrees C on subsequent long-term culture, which evaluates both hematopoietic precursor cells and hematopoietic microenvironmental cells. Storage of unfractionated marrow was superior to storage of buffy-coat cells in tissue culture medium with 20% fetal calf serum. CFU-C recovery in unfractionated marrow was 48.4% at four days and 21.4% at seven days. Long-term marrow cultures from cells stored at 4 degrees C for up to seven days produced CFU-C for up to seven weeks and established confluent marrow stromal cell layers. Suspension cultures of marrow cells preserved at 4 degrees C for seven days cultured with irradiated allogeneic marrow stromal cell layers from normal long-term marrow cultures showed significantly increased CFU-C production from week 2 to week 5 when compared with the control cultures without adherent cell layers. These data suggest that marrow storage at 4 degrees C for up to seven days preserves early hematopoietic precursor cells and microenvironmental cells and may be used for autologous rescue from marrow ablative therapy.
D001853:Bone Marrow / Q000528:radiation effects; D001854:Bone Marrow Cells; D002452:Cell Count; D002469:Cell Separation; D002470:Cell Survival; D002478:Cells, Cultured; D003080:Cold Temperature; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D013997:Time Factors; D014021:Tissue Preservation