Enzymatic fluorometric method for the determination of D-arabinitol in serum by initial rate analysis.
We describe a new, simple, fluorometric assay for D-arabinitol in serum. The method is based on oxidation of D-arabinitol by D-arabinitol dehydrogenase (EC 1.1.1.11), with the concomitant reduction of NAD. The initial rate of NAD reduction, which is proportional to the D-arabinitol content of serum, can be measured with a recording spectrofluorometer. Sensitivity, specificity, recovery and reproducibility experiments gave satisfactory results. The proposed method is suitable for clinical use, and may be helpful in the diagnosis of invasive candidiasis.
D000328:Adult; D002177:Candidiasis / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D013050:Spectrometry, Fluorescence; D013401:Sugar Alcohol Dehydrogenases / Q000378:metabolism; D013402:Sugar Alcohols / Q000097:blood*
D013402:Sugar Alcohols; D013401:Sugar Alcohol Dehydrogenases; C022751:D-arabinitol dehydrogenase; C014999:arabitol