Formation of single-stranded regions in the course of digestion of DNA with DNAase II and micrococcal nuclease.
In the course of digestion of DNA with DNAase II or micrococcal nuclease, considerable amounts of single-stranded (ss) regions are formed, as determined by a second digestion with ss-specific nucleases, hyperchromicity measurements, and electron microscopy. Most of the ss stretches are located internally in the DNA molecules. The effect appears to be related to regions of decreased stability arising around single-stranded cuts in the double helix.
D000818:Animals; D002843:Chromatin / Q000378:metabolism; D002848:Chromatography, DEAE-Cellulose; D004247:DNA; D004277:DNA, Single-Stranded / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D004706:Endodeoxyribonucleases; D007887:Fabaceae; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D051379:Mice; D008836:Micrococcal Nuclease; D009691:Nucleic Acid Denaturation; D010946:Plants, Medicinal; D012995:Solubility
D002843:Chromatin; D004277:DNA, Single-Stranded; D004247:DNA; D004706:Endodeoxyribonucleases; C019329:deoxyribonuclease II; D008836:Micrococcal Nuclease