An apparatus for the concentration of large volumes of dilute protein solutions to a predetermined volume.
An apparatus was designed and manufactured that can be used for the concentration of large quantities of dilute protein solutions to a predetermined volume. The method is based on the osmotic transfer of water to a concentrated hydrophilic polymer (poly ethylene glycol, PEG) through a protein-stopping dialysis membrane and is a refinement of a method previously reported by van Oss. The apparatus is made of perspex. Concentration takes place through a commercially available dialysis tube and is aided by a 40% polyethylene glycol solution. Large volumes (5l) of dilute protein solution could be reduced to 50 ml at a rate of 30 ml per hour with no significant loss in biological activity.
D000818:Animals; D001671:Biochemistry / Q000295:instrumentation*; D003577:Cytochrome P-450 Enzyme System / Q000302:isolation & purification; D003956:Dialysis / Q000295:instrumentation; D011506:Proteins / Q000302:isolation & purification*; D012756:Sheep
D011506:Proteins; D003577:Cytochrome P-450 Enzyme System