title: Metabolic studies of explosives. II. High-performance liquid chromatography-mass spectrometry of metabolites of 2,4,6-trinitrotoluene.
abstract: A series of metabolites of 2,4,6-trinitrotoluene were studied by combined high-performance liquid chromatography-mass spectrometry. These metabolites are formed mainly by oxidation and reduction processes. Separations were done on a C8 reversed-phase column, using acetonitrile-water at various relative concentrations as mobile phases, followed by ultraviolet and on-line mass spectrometry with a direct liquid insertion probe liquid chromatograph-mass spectrometer interface. The mass spectra obtained were chemical-ionization spectra with the mobile phase as reagent. Mass spectra obtained included mainly reduced ions, adduct ions and typical fragment ions. The combination of high-performance liquid chromatographic separation with on-line mass spectrometry was found to be a suitable method for the identification of the investigated metabolites.
mesh_terms: D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D008297:Male; D013058:Mass Spectrometry; D051381:Rats; D011919:Rats, Inbred Strains; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D014303:Trinitrotoluene / Q000378:metabolism*
chemical_list: D012997:Solvents; D014303:Trinitrotoluene