Detection of anaerobic bacteria in blood cultures by lysis filtration.
An anaerobic adaptation of the lysis-filtration system for detection of anaerobic microorganisms in blood is described. The method was compared with a conventional broth bottle system in detection of anaerobic bacteremia after oral surgery. Of 43 blood samples obtained during and after surgery, 31 were positive with the lysis-filtration system and 17 were positive with the broth bottle system. Sixteen aerobic and 62 anaerobic strains were isolated with the lysis-filtration system versus 9 aerobic and 22 anaerobic strains with the broth bottles. The lysis-filtration technique was thus superior to the conventional broth bottle method in detecting anerobic bacteria.
D000328:Adult; D000368:Aged; D001421:Bacteria, Anaerobic / Q000302:isolation & purification*; D001769:Blood / Q000382:microbiology*; D004917:Erythromycin / Q000627:therapeutic use; D005260:Female; D005374:Filtration; D006801:Humans; D008297:Male; D008875:Middle Aged; D018805:Sepsis / Q000175:diagnosis / Q000517:prevention & control; D014081:Tooth Extraction
D004917:Erythromycin