title: Heligmosomoides polygyrus: inoculum size and glucose, ion, and gas fluxes in the small intestine of mice.
abstract: Female CDI mice were inoculated with 10, 50, 100, 250, or 500 larvae of Heligmosomoides polygyrus. At Days 7, 9, and 12 after infection, the anterior third of the small intestine was perfused using an in vivo technique. The distribution of worms in the mouse intestine was determined after 7, 9, and 12 days. All worms that were recovered were from the proximal half of the small intestine. When compared to uninfected controls, there was a significant increase (+56%) in glucose absorption of the small intestine at Day 7 after infection with inocula of 50 and 100 larvae; at Day 9, glucose absorption was significantly increased with a 10-larvae inoculum. A decrease in glucose absorption occurred at Days 7 and 9 after infection with a 500-larvae inoculum. Net water absorption was significantly increased (+183%) with the 50- and 100-larvae inocula at Day 7, but was significantly reduced at Day 9 after infection with the 50-, 100-, 250-, and 500-larvae inocula. Both Cl- and Na+ absorption were significantly increased with the 50-, 100-, and 250-larvae inocula at Day 7 after infection; at 9 and 12 days, there was significant net secretion of both ions. In control mice, there was net secretion of K+, while with the 50-, 100-, and 250-larvae inocula on Day 7 there was significant net absorption of K+ ions.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000818:Animals; D004573:Electrolytes / Q000378:metabolism*; D005260:Female; D005740:Gases / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D007408:Intestinal Absorption; D007411:Intestinal Diseases, Parasitic / Q000378:metabolism*; D007421:Intestine, Small / Q000378:metabolism* / Q000469:parasitology; D007814:Larva; D051379:Mice; D013997:Time Factors; D014252:Trichostrongyloidiasis / Q000378:metabolism*; D014867:Water / Q000378:metabolism
chemical_list: D004573:Electrolytes; D005740:Gases; D014867:Water; D005947:Glucose