Release of T-kinin and bradykinin in carrageenin induced inflammation in the rat.
Plasma and inflammatory fluid kininogen levels, and blood and inflammatory fluid free kinin levels were determined in rats 24 h after the injection of carrageenin into an air pouch. Plasma T-kininogen levels increased 7-fold. In the inflammatory fluid levels reached 8 micrograms/ml. Blood levels of free kinin showed a 5-fold increase. The kinins were identified on HPLC as T-kinin (Ile-Ser-bradykinin) and bradykinin, 63 and 37%, respectively. These results indicate for the first time that free T-kinin as well as bradykinin is released during an inflammatory response in rat and confirms our previous finding that T-kininogen may be a major acute-phase protein in inflammation.
D000818:Animals; D001920:Bradykinin / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism*; D002351:Carrageenan; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007249:Inflammation / Q000097:blood / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D010949:Plasma / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
C039065:T-kinin; D002351:Carrageenan; D001920:Bradykinin