title: The hierarchy of requirements for an elevated intracellular pH during early development of sea urchin embryos.
abstract: The intracellular pH (pHi) rises 0.3-0.5 units after fertilization of sea urchin eggs, and this and previous work show this pHi change is necessary for initiating the developmental processes leading to cell division. The experiments described here reveal that while the elevated pHi is permanently required for a normal early development, lowering pHi of embryos after fertilization affects different processes to different extents. Protein synthesis gradually becomes less sensitive to pHi. Karyokinesis proceeds to completion under a low pHi, but is retarded, while cytokinesis is always impaired. These results indicate a hierarchy of requirements for high pHi during early development of sea urchin embryos, with protein synthesis, karyokinesis, and cytokinesis showing, respectively, increasing requirements for an elevated pHi.
mesh_terms: D000085:Acetates / Q000494:pharmacology; D019342:Acetic Acid; D000584:Amiloride / Q000494:pharmacology; D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D005260:Female; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008938:Mitosis / Q000187:drug effects; D014176:Protein Biosynthesis; D012617:Sea Urchins / Q000196:embryology*; D012964:Sodium / Q000494:pharmacology; D015053:Zygote / Q000187:drug effects / Q000378:metabolism
chemical_list: D000085:Acetates; D000643:Ammonium Chloride; D000584:Amiloride; D012964:Sodium; D019342:Acetic Acid