Assay of beta-glucuronidase in bile following ion-pair extraction of pigments and bile acids.
A method for improving the assay of beta-glucuronidase in hepatic and gallbladder bile is described. The method uses ion-pair extraction with N,N,N-triheptyl-1-heptanaminium bromide to remove pigments and bile acids. Conjugated bilirubin, unconjugated bilirubin, and taurine and glycine conjugates of deoxycholic and chenodeoxycholic acids are extracted efficiently from bile by the procedure. The sensitivity of the spectrophotometric assay of beta-glucuronidase in bile using phenolphthalein glucuronide is increased significantly.
D000818:Animals; D001646:Bile / Q000201:enzymology*; D001647:Bile Acids and Salts / Q000302:isolation & purification*; D001654:Bile Pigments / Q000302:isolation & purification*; D005966:Glucuronidase / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007474:Ion Exchange; D010635:Phenolphthaleins / Q000378:metabolism; D013552:Swine; D013556:Swine, Miniature
D001647:Bile Acids and Salts; D001654:Bile Pigments; D010635:Phenolphthaleins; C016651:phenolphthalein glucuronide; D005966:Glucuronidase