Catechol oestrogen induced initiation of implantation in the delayed implanting rat.
In the rat oestrogen is essential for induction of implantation in a progesterone primed uterus. The ability of the catechol oestrogens, 4-hydroxy-oestradiol (4-OH-E2) and 2-hydroxy-oestradiol (2-OH-E2) to initiate the implantation process (blue reaction, indicative of increased endometrial capillary permeability at the location of the blastocyst) in hypophysectomized delayed implanting rats was compared to that of oestradiol-17 beta (E2). Delayed implantation was maintained by daily administration of 2 mg of progesterone. A single sc injection of 100 ng of E2 or 400 ng of 4-OH-E2 consistently initiated implantation in all animals. When delivered sc via osmotic minipumps at a constant release rate of 10 ng/microliters/h, implantation of a full complement of embryos was obtained with E2 given for 6 or 24 h. This dose 4-OH-E2 was ineffective when administered for 24 h. However, 25 ng/microliters/h for 24 h was effective. When the dose was increased to 50 ng/microliters/h implantation was evident in the majority of animals exposed to the hormone for 6 or 8 h; all animals implanted when this dose was given for 24 h. In contrast, 2-OH-E2 given at 50 ng/microliters/h for 24 h was totally ineffective. When the dose of the latter steroid was raised to 200 ng/microliters/h implantation was achieved in half of the animals when exposure was 24 h. The results suggest that when administered systemically, 4-OH-E2 is less potent than E2, but more potent than 2-OH-E2, for initiating implantation.(ABSTRACT TRUNCATED AT 250 WORDS)
D000818:Animals; D001755:Blastocyst / Q000187:drug effects; D002199:Capillary Permeability / Q000187:drug effects; D010064:Embryo Implantation / Q000187:drug effects*; D010065:Embryo Implantation, Delayed / Q000187:drug effects*; D004717:Endometrium / Q000098:blood supply; D004958:Estradiol / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D002393:Estrogens, Catechol; D005260:Female; D007016:Hypophysectomy; D007279:Injections, Subcutaneous; D011247:Pregnancy; D051381:Rats
D002393:Estrogens, Catechol; D004958:Estradiol; C001390:2-hydroxyestradiol; C014036:4-hydroxyestradiol