title: [Influence of intraarticular continuous perfusion of physiological saline on chondrocytes].
abstract: Physiological saline, which is generally used in arthroscopic examination and operation, is not physiological in the synovial joints. This paper discusses metabolic changes in the articular cartilage following continuous intraarticular perfusion with physiological saline, in comparison with in vitro findings. Fourty rabbits weighing approximately 3 kg were intraarticularly and continuously perfused with saline at a rate of 250 ml/hour. Articular cartilage of the femoral condyle was collected for measurement of 35SO4 radioactivity by liquid-scintillation spectrometry. Incorporation of 35SO4 showed increment in perfused articular cartilage as compared with the controls after one hour. Namely, glycosaminoglycan synthesis of chondrocytes was accelerated temporarily by continuous perfusion of saline, and it was recovered twenty-four hours after perfusion. In addition, histological and biomechanical test showed no remarkable changes. But, these effects will be expanded by the presence of degenerated articular cartilage. Continuous perfusion of physiological saline changes the physiological circumstance for articular cartilage, and influences on the chondrocytes' metabolism temporarily.
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis; D066298:In Vitro Techniques; D007552:Isotonic Solutions; D007596:Joints; D008297:Male; D010477:Perfusion; D011817:Rabbits; D012965:Sodium Chloride / Q000008:administration & dosage / Q000009:adverse effects*
chemical_list: D006025:Glycosaminoglycans; D007552:Isotonic Solutions; D012965:Sodium Chloride