The effect of 3H-thymidine on the proliferation of in vitro cultured mammalian cells.
The effect of 3H-thymidine on the proliferation of Chinese hamster cells (clone V79) was studied. Following 3H-thymidine application the proliferation of cells (studied on the basis of plating efficiency) was found to be diminished, the drop being dependent on radioactivity (2-20 kBq/ml cultivation medium), the time of application (2-20 h) and specific activity of 3H-thymidine added. Exogenous macromolecular DNA was able to repair, to an important degree the radiotoxic effect of 3H-thymidine on V79 cells by a mechanism other than the mere reduction of specific activity of 3H-thymidine.
D000818:Animals; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects*; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000494:pharmacology / Q000528:radiation effects; D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects*; D008168:Lung; D008297:Male; D013936:Thymidine / Q000378:metabolism / Q000494:pharmacology*; D014316:Tritium
D014316:Tritium; D004247:DNA; D013936:Thymidine