Microbore packed-column anion-exchange liquid chromatography of nucleobases, nucleosides and nucleotides.
Low-capacity anion-exchange resin was packed into a 45 cm X 0.19 mm I.D. fused-silica tubing and applied to micro-column liquid chromatography of nucleobases, nucleosides and isomers of nucleotides. The effects of the chromatographic conditions on the elution behaviour of these compounds were studied. The efficiency of the microbore packed column was comparable with that of the conventional size column.
D000837:Anion Exchange Resins; D002021:Buffers; D002852:Chromatography, Ion Exchange / Q000379:methods; D007536:Isomerism; D009705:Nucleosides / Q000032:analysis*; D009711:Nucleotides / Q000032:analysis*; D010710:Phosphates; D011687:Purines / Q000032:analysis*; D011743:Pyrimidines / Q000032:analysis*; D013696:Temperature
D000837:Anion Exchange Resins; D002021:Buffers; D009705:Nucleosides; D009711:Nucleotides; D010710:Phosphates; D011687:Purines; D011743:Pyrimidines