title: Detection of impervious tissue in tree bark with selective histochemistry and fluorescence microscopy.
abstract: Use of conventional histochemical tests in conjunction with fluorescence microscopy has validated the concept of impervious tissue in the bark of trees. Application of phloroglucinol + HCl or toluidine blue O selectively quenched lignin autofluorescence and allowed visualization of intracellular suberin lamellae previously undetected. Fluorescence of intracellular lamellae was quenched with Sudan black B and enhanced with Sudan IV thus providing evidence for the suberized nature of a tissue heretofore regarded as nonsuberized.
mesh_terms: D006651:Histocytochemistry / Q000379:methods*; D008031:Lignin / Q000032:analysis; D008856:Microscopy, Fluorescence / Q000379:methods*; D059828:Plant Cells; D014197:Trees
chemical_list: D008031:Lignin