[The effect of modification of the intracellular distribution of polyamines on the proliferation of hepatocytes treated with 2-methyl-9-hydroxyellipticine].
2-Methyl-9-hydroxyellipticine (MHE) added to tumoral hepatocyte cultures (400 ng MHE/ml medium) is responsible for a cytostatic effect. This decrease of cellular proliferation and of DNA synthesis is accompanied by an increase of intracellular free spermidine levels. We could not attribute this result to a deviation of this polyamine metabolism. These increased concentrations of free spermidine are connected to a decrease of the amount of nuclear bound polyamines, mainly joined with the RNA fraction. Thus, the cytostatic effect observed with this drug might be related to a decrease in the amount of spermidine connected to RNA; therefore, its intercalating effect is only indirectly responsible for the cellular proliferation decrease.
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004273:DNA, Neoplasm / Q000378:metabolism; D004611:Ellipticines / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology*; D011073:Polyamines / Q000378:metabolism*; D013095:Spermidine / Q000378:metabolism
D000470:Alkaloids; D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D004611:Ellipticines; D011073:Polyamines; C027767:elliptinium; D013095:Spermidine
Influence d'une modification de la repartition intracellulaire des polyamines sur la proliferation d'hepatocytes traites par la methyl-2-hydroxy-9 ellipticine.