Effect of thiol-reactive reagents on the permeability of fish erythrocyte membrane for spin-labelled non-electrolytes.
Carp erythrocytes were treated with p-chloromercuribenzoate or N-ethylmaleimide. It was observed that these thiol-group inhibitors decreased the transport of spin-labelled hydrophilic compound, 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl, and increased the transport rate of more hydrophobic 2,2,6,6-tetramethylpiperidine-1-oxyl.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002347:Carps; D002463:Cell Membrane Permeability / Q000187:drug effects*; D003497:Cyclic N-Oxides / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000187:drug effects*; D007700:Kinetics; D010880:Piperidines / Q000378:metabolism; D013113:Spin Labels; D013329:Structure-Activity Relationship; D013439:Sulfhydryl Reagents / Q000494:pharmacology*
D003497:Cyclic N-Oxides; D010880:Piperidines; D013113:Spin Labels; D013439:Sulfhydryl Reagents; C001803:tempol; C003959:TEMPO