Early transmembrane events in tumour cell responses observed by stopped-flow fluorometry.
Early transmembrane events of tumour cells (mouse myeloma X5563 and lymphoma RDM4) after binding of a monoclonal antibody against mouse MHC antigen and a mitogenic lectin, Con A, were examined by stopped-flow fluorometry with 3 different fluorescent probes. The results showed that membrane fluidities of the cells increased first after binding of anti H-2Kk monoclonal antibody (11-4.1), then calcium was released from intracellular stores into the cytoplasma, and lastly calcium influx occurred from the external medium into the cytoplasma. While Con A only induced calcium influx from the external medium into the cytoplasma.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology*; D003208:Concanavalin A / Q000276:immunology; D003593:Cytoplasm / Q000378:metabolism; D006183:H-2 Antigens / Q000276:immunology; D008223:Lymphoma / Q000503:physiopathology; D008560:Membrane Fluidity; D051379:Mice; D009101:Multiple Myeloma / Q000503:physiopathology; D009374:Neoplasms, Experimental / Q000503:physiopathology*; D013050:Spectrometry, Fluorescence
D000911:Antibodies, Monoclonal; D006183:H-2 Antigens; D003208:Concanavalin A; D002118:Calcium