[Fructose utilization by hepatocytes isolated from fed obese fa/fa Zucker rats. Metabolic fate of fructose in the absence of the modulator].
The utilization of fructose by isolated hepatocytes was investigated in fed obese Zucker fa/fa rats compared with their lean littermates (Fa/?) and Sprague-Dawley rats. Hepatocytes were incubated during 3 h using U14C fructose (20 mM). Our results show: a significant increase of fructose consumption, glucose, lactate and pyruvate production and faster turnover of glycogen by fa/fa rats. In these animals, synthesis of acylglycerol was also significantly enhanced. Our results suggest that fructose in fa/fa rats was used preferentially as precursor for lipid synthesis not only by the liver but also by the adipose tissue after a prior transformation into glucose by hepatocytes. All these abnormalities result in an accumulation of acylglycerols maintaining an obesity state in fa/fa rats.
D000818:Animals; D005632:Fructose / Q000378:metabolism*; D005947:Glucose / Q000032:analysis; D005990:Glycerol / Q000032:analysis; D007773:Lactates / Q000032:analysis; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008112:Liver Glycogen / Q000096:biosynthesis; D008297:Male; D011773:Pyruvates / Q000032:analysis; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains / Q000378:metabolism*; D011922:Rats, Mutant Strains / Q000378:metabolism*; D011924:Rats, Zucker / Q000378:metabolism*
D007773:Lactates; D008112:Liver Glycogen; D011773:Pyruvates; D005632:Fructose; D005947:Glucose; D005990:Glycerol
Utilisation du fructose par l'hépatocyte isolé de rat nourri Zucker obèse fa/fa. I. Orientation métabolique du fructose en l'absence de modulateur.