title: Alterations in the physical state and composition of brush border membrane lipids of rat enterocytes during differentiation.
abstract: The physical state of the membrane lipid of brush border membranes, prepared from rat small intestinal villus and crypt cells, was examined by steady-state fluorescence polarization using three lipid-soluble fluorophors. Membranes prepared from crypt cells were found to possess a higher lipid fluidity than those of villus cells with each probe. Analysis of the composition of these membranes revealed that those from crypt cells had lower ratios of cholesterol/phospholipid (mol/mol), protein/lipid (w/w), and saturated fatty acyl chains/unsaturated chains (w/w). Alterations in the levels of stearic (18:0) and oleic (18:1) acids were responsible for differences in the latter ratio. The results, therefore, demonstrate that alterations in the lipid composition and fluidity of brush border membranes of enterocytes occur during the process of differentiation.
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D005227:Fatty Acids / Q000378:metabolism; D005454:Fluorescence Polarization; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D007421:Intestine, Small / Q000166:cytology / Q000378:metabolism; D008297:Male; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000378:metabolism*; D008871:Microvilli / Q000378:metabolism; D051381:Rats
chemical_list: D005227:Fatty Acids; D008563:Membrane Lipids