Localization of the enantiozymes of 6-hydroxy-nicotine oxidase in Arthrobacter oxidans by electron immunochemistry.
During the course of growth of Arthrobacter oxidans, induction of the enantiozymes 6-hydroxy-D-nicotine oxidase and 6-hydroxy-L-nicotine oxidase occurred in the presence of DL-nicotine. Cryoultramicrotomed sections obtained from cells grown to stationary phase were gold immunolabeled. The results obtained demonstrate that both enzymes are localized in the cytoplasm.
D001173:Arthrobacter / Q000201:enzymology* / Q000648:ultrastructure; D007106:Immune Sera; D007118:Immunoassay; D008854:Microscopy, Electron; D010088:Oxidoreductases / Q000032:analysis*; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000032:analysis*
D007106:Immune Sera; D010088:Oxidoreductases; D000587:Oxidoreductases Acting on CH-NH Group Donors; C037584:6-hydroxy-L-nicotine oxidase; C021413:6-hydroxy-D-nicotine oxidase