[Equilibrium characteristics of calcium binding on the inner surface of membrane vesicles].
A method is proposed for determining equilibrium parameters of calcium binding on the inner surface of membrane vesicules. Based on the studies of concentration relationship of calcium content in vesicules of myometrium sarcolemma (at the equilibrium state) there were determined the values of maximal capacity of Ca2+-binding (12.79 nmole/mg of protein) and the dissociation constants of Ca2+-binding centre complex (62.3 mkM) on the inner surface of the vesicules, and the value of intravesicular volume as well (25.7 mkl/mg of protein).
D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D005260:Female; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000378:metabolism*; D007700:Kinetics; D009215:Myometrium / Q000378:metabolism; D012508:Sarcolemma / Q000378:metabolism
D002118:Calcium
Ravnovesnye kharakteristiki sviazyvaniia kal'tsiia na vnutrenneĭ poverkhnosti membrannykh vezikul.