[Characteristics of nuclear DNA of a Lepidoptera, the great wax moth Galleria mellonella, using reassociation kinetics].
Reassociation kinetics of great wax moth--Galleria mellonella nuclear DNA short fragments was studied within the C0t values of 0.02 and 10(4). The least squares analysis of the experimental curve obtained revealed two components with C0t1/2 = 0.72 and C0t1/2 = 750 appropriately. The fraction of the component with C0t1/2 = 0.72 in the genome of great wax moth is 22% and that of the other one is 61%. There is also a rapid component which renatured at the C0t values less then 0,02. The fraction of this component in the genome is 17%. The great wax moth genome size determined kinetically is 4,83 X 10(8) base pairs. It was shown that the efficiency of DNA molecules network formation can qualify the genome studied as being a short or a long period of interspersion. On the basis that 80-90% of great wax moth DNA molecules participated in network formation which is inherent to the short period interspersion genomes, it was concluded that the great wax moth genome is of the Xenopus-like type.
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D007915:Lepidoptera / Q000235:genetics*; D009036:Moths / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
D004247:DNA
Kharakteristika iadernoĭ DNK cheshuekrylogo nasekomogo--bol'shoĭ voshchinnoĭ moli Galleria mellonella s pomoshch'iu kinetiki reassotsiatsii.