title: Absence of filipin-sterol complexes from the ciliary necklace of ependymal cells.
abstract: The polyene antibiotic filipin reacts specifically with membrane cholesterol and produces membrane perturbations. Prefixed vibratome slices of cat ependymal cells were treated with a glutaraldehyde solution containing 300 microM filipin. Freeze-fracture of these cells revealed numerous 25-30 nm protuberances on fracture faces (filipin-sterol complexes) in the ciliary membrane. In the ciliary necklace, however, these filipin-sterol complexes were absent. These results suggest that the ciliary necklace has a low cholesterol content.
mesh_terms: D000818:Animals; D002415:Cats; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002784:Cholesterol / Q000378:metabolism*; D002923:Cilia / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D004805:Ependyma / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D005372:Filipin; D005614:Freeze Fracturing; D008854:Microscopy, Electron; D011090:Polyenes
chemical_list: D011090:Polyenes; D005372:Filipin; D002784:Cholesterol