Proton nuclear magnetic resonance assignments of thyroid hormone and its analogues.
1H NMR data of a series of thyroid hormone analogues, e.g., thyroxine (T4), 3,5,3'-triiodothyronine (T3), 3,3',5'-triiodothyronine (rT3), 3,3'-diiodothyronine (3,3'-T2), 3,5-diiodothyronine (3,5-T2), 3',5'-diiodothyronine (3',5'-T2), 3-monoidothyronine (3-T1), 3'-monoiodothyronine (3'-T1), and thyronine (TO) in dimethylsulfoxide (DMSO) have been obtained on a 300 MHz spectrometer. The chemical shift and coupling constant are determined and tabulated for each aromatic proton. The inner tyrosyl ring protons in T4, T3, and 3,5-T2 have downfield chemical shifts with respect to those of the outer phenolic ring protons. Four-bond cross-ring coupling has been observed in all the monoiodinated rings. However, this long-range coupling does not exist in T4, diiodinated on both rings, and T0, containing no iodines on the rings. There is no evidence that at 30 degrees C these iodothyronines have any motional constraint in DMSO solution. In addition to identification of the hormones, the potential use of some characteristic peaks as probes in binding studies is discussed.
D004104:Diiodothyronines; D009682:Magnetic Resonance Spectroscopy; D013963:Thyroid Hormones; D013970:Thyronines; D013974:Thyroxine; D014284:Triiodothyronine; D014285:Triiodothyronine, Reverse
D004104:Diiodothyronines; D013963:Thyroid Hormones; D013970:Thyronines; D014284:Triiodothyronine; C013068:3-monoiodothyronine; C019017:3'-monoiodothyronine; C030103:3,5-diiodothyronine; D014285:Triiodothyronine, Reverse; C030102:3',5'-diiodothyronine; C030629:3,3'-diiodothyronine; D013974:Thyroxine