Detection of the tripeptide Tyr-Gly-Gly, a putative enkephalin metabolite in brain, using a sensitive radioimmunoassay.
A sensitive and specific radioimmunoassay has been developed for YGG. The tripeptide was previously derivatised with p-benzoquinone to prepare the immunogen and the 125I tracer as well as in samples submitted to the RIA. The sensitivity is about 1 nM as compared with 8000 nM for underivatised YGG. Measurable amounts of endogenous YGG immunoreactivity, co-eluting in HPLC with authentic YGG, were detected in mouse striatal extracts.
D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D004745:Enkephalins / Q000378:metabolism*; D051379:Mice; D009842:Oligopeptides / Q000032:analysis*; D011863:Radioimmunoassay
D004745:Enkephalins; D009842:Oligopeptides; C044757:tyrosyl-glycyl-glycine