[Identification of pro-opiomelanocortin and the secretion of its peptide fragments in the adrenals of the bull].
Immunoreactive alpha-, beta- and gamma-endorphins and beta-lipotropin--C-terminal peptide fragments of pro-opiomelanocortin (POMC)--were discovered and measured by RIA in the bovine adrenal medulla and cortex. These peptides were also discovered in perfusates of the adrenal gland. POMC proper and some intermediate forms of its processing not differing in electrophoretic mobility from the respective molecular forms of hypophyseal POMC were identified in the medulla and cortex of the adrenals by the immunoblotting technique with the use of antiserum to beta-lipoprotein. It is concluded that POMC gene is expressed in the adrenal medulla and cortex and that as a result of POMC processing a noticeable amount of its peptide fragments is formed and secreted in adrenal cells. The authors thus suggest the presence of existence of the pituitary-unrelated mechanisms of adrenal function control with participation of POMC peptides synthesized in the adrenals.
D000311:Adrenal Glands / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D004723:Endorphins / Q000032:analysis; D008297:Male; D010446:Peptide Fragments / Q000032:analysis* / Q000378:metabolism; D011333:Pro-Opiomelanocortin / Q000032:analysis* / Q000096:biosynthesis; D011863:Radioimmunoassay / Q000379:methods; D008083:beta-Lipotropin / Q000032:analysis
D004723:Endorphins; D010446:Peptide Fragments; D011333:Pro-Opiomelanocortin; D008083:beta-Lipotropin
Identifikatsiia proopiomelanokortina i sekretsiia ego peptidnykh fragmentov v nadpochechnikakh byka.