Inhibition by potential metabolic inhibitors of in vitro adipose tissue lipogenesis.
To study the pathway of lactate utilization as a carbon source for fatty acid synthesis, the effect of (-)-hydroxycitrate, agaric acid, sodium oxamate, 2-n-butyl malonate and alpha-cyano-4-hydroxycinnamate on the rate of in vitro conversion of lactate, acetate and glucose to fatty acids was measured in bovine and rat adipose tissues. Sodium oxamate and hydroxycitrate caused less fatty acid to be synthesized from lactate in bovine adipose tissue. Hydroxycitrate depressed fatty acid synthesis from glucose in rat adipose tissue. alpha-Cyano-4-hydroxycinnamate was an effective inhibitor of lipogenesis from all substrates and may act as a specific inhibitor in adipose tissue. Although the inhibitors were absorbed poorly into adipocytes, the results indicate that conversion of lactate to fatty acids probably occurs by way of the citrate cleavage pathway.
D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001224:Aspartic Acid / Q000494:pharmacology; D002250:Carbon Radioisotopes; D002264:Carboxylic Acids / Q000494:pharmacology*; D002417:Cattle; D002951:Citrates / Q000494:pharmacology; D019343:Citric Acid / Q000031:analogs & derivatives*; D003373:Coumaric Acids / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008055:Lipids / Q000096:biosynthesis*; D008297:Male; D008314:Malonates / Q000494:pharmacology; D010072:Oxamic Acid / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
D000085:Acetates; D002250:Carbon Radioisotopes; D002264:Carboxylic Acids; D002951:Citrates; D003373:Coumaric Acids; D007773:Lactates; D008055:Lipids; D008314:Malonates; D019343:Citric Acid; C011396:agaric acid; D001224:Aspartic Acid; D019344:Lactic Acid; C006860:2-n-butylmalonate; C018254:alpha-cyano-3-hydroxycinnamate; C007999:hydroxycitric acid; D005947:Glucose; D019342:Acetic Acid; D010072:Oxamic Acid