Comparison of enzymatic characteristics of creatine kinase BB from human healthy and tumor stomach tissues.
Creatine kinase (ATP: creatine N-phosphotransferase, EC 2.7.3.2, CK) BB isoenzyme from stomach tumor tissue was partially purified and its characteristics were compared with those from healthy tissue. Molecular mass of tumor CK-BB was estimated to be 82 000 by polyacrylamide gel electrophoresis. Tumor CK-BB was separated into 2 main subbands around pH 4.5 and 11, minor subbands around pH 5-7.5 by agarose isoelectric focusing. The isoenzyme reacted with anti-human brain CK-BB antibodies and formed a hybrid, CK-MB, with CK-MM prepared from healthy human skeletal muscle. The above physicochemical and immunological characteristics of tumor CK-BB were the same as those of normal CK-BB from normal stomach tissue. Optimum pH of tumor CK-BB was more acidic than that of normal CK-BB. Affinity for creatine phosphate and heat sensitivity of tumor CK-BB were slightly lower than those of normal CK-BB. Tumor CK-BB was more stable after iodoacetamide and urea treatments.
D001921:Brain / Q000201:enzymology; D003402:Creatine Kinase / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007460:Iodoacetamide / Q000494:pharmacology; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000378:metabolism*; D008970:Molecular Weight; D009132:Muscles / Q000201:enzymology; D013270:Stomach / Q000201:enzymology*; D013274:Stomach Neoplasms / Q000201:enzymology*; D013816:Thermodynamics; D014508:Urea / Q000494:pharmacology
D007527:Isoenzymes; D014508:Urea; D003402:Creatine Kinase; D007460:Iodoacetamide