A combination fluorescence assay and Folin-Ciocalteau phenol reagent assay for the detection of paralytic shellfish poisons.
Paralytic shellfish poison (PSP) profiles of crude shellfish extracts were determined by linear gradient elution from a Bio-Rad AG-50-X4 strong cation-exchange resin mini-column. STX, GTX2 and GTX3 were detected by fluorescence assay. NeoSTX and GTX1/GTX4 were detected with a Folin-Ciocalteau phenol reagent assay. The major toxicity associated with extracts of Mytilus edulis and Mya arenaria collected during a 1972 red tide off Hampton, New Hampshire, was due to the presence of GTX1/GTX4, with some activity associated with neoSTX, GTX2 and GTX3. STX was also present. Correlations to mouse toxicity are provided.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D049872:Bivalvia / Q000378:metabolism; D007202:Indicators and Reagents; D008387:Marine Toxins / Q000032:analysis* / Q000633:toxicity; D051379:Mice; D008982:Molybdenum; D010243:Paralysis / Q000139:chemically induced; D012530:Saxitoxin / Q000032:analysis; D012758:Shellfish / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013055:Spectrophotometry, Infrared; D014414:Tungsten; D017973:Tungsten Compounds
D000596:Amino Acids; C029556:Folin's phenol reagent; D007202:Indicators and Reagents; D008387:Marine Toxins; D017973:Tungsten Compounds; D012530:Saxitoxin; D008982:Molybdenum; D014414:Tungsten