A spectrophotometric resin competition procedure to determine dissociation constants for metal ion-nucleotide complexes.
The resin competition method for determining the dissociation constants of metal ion-nucleotide complexes was modified to take into account the fact that some metal nucleotide complexes are anionic and thus, like the free nucleotide, will bind to the resin. A simple, rapid spectrophotometric titration procedure is given for the determination of both the metal ion-nucleotide complex dissociation constant and the ratio of the affinities of the nucleotide and its complex for the resin.
D001665:Binding Sites; D001667:Binding, Competitive; D002021:Buffers; D055598:Chemical Phenomena; D002621:Chemistry; D007475:Ion Exchange Resins; D007700:Kinetics; D008433:Mathematics; D008670:Metals / Q000032:analysis*; D008956:Models, Chemical; D009711:Nucleotides / Q000032:analysis*; D013053:Spectrophotometry
D002021:Buffers; D007475:Ion Exchange Resins; D008670:Metals; D009711:Nucleotides