Potentiality of the murine colony-forming cells detected by an in vivo diffusion chamber culture system.
Culture of a mixture of bone marrow cells with and without T6 chromosome marker in diffusion chambers in mice yielded colonies (CFU-DG) containing cells of a single karyotype, suggesting clonality. Injection of individual CFU-DG colonies into lethally irradiated mice resulted in increased spleen colony formation on day 12 (CFU-S). The possibility of endogenous origin was excluded by demonstrating the presence of T6 marker in both CFU-DG and CFU-S colonies in karyotypically normal hosts. These findings suggest that the cells giving rise to granulocytic colonies in diffusion chambers also can give rise to multipotential hemopoietic cells.
D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D003114:Colony-Forming Units Assay; D046508:Culture Techniques / Q000379:methods; D004058:Diffusion; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D007621:Karyotyping; D051379:Mice; D008813:Mice, Inbred ICR; D011336:Probability; D013154:Spleen / Q000166:cytology