Immunoelectron microscopic localization of growth hormone in the pituitary glands of two teleosts, tilapia (Sarotherodon mossambicus) and amago salmon (Oncorhynchus rhodurus).
Growth hormone (GH) cells were investigated with the protein A-gold technique on the pituitary glands of tilapia (Sarotherodon mossambicus) and amago salmon (Oncorhynchus rhodurus). By the use of specific antiserum against tilapia GH to both species, the immunoreactive gold particles were demonstrated to be preferentially located on the secretory granules of the GH cells. Specimens fixed only with periodate-lysine-paraformaldehyde (PLP) preserved the hormonal antigenicity well. Osmium postfixation, although considerably reducing the antigenicity and thus resulting in a decrease in number of the gold particles on the GH cells, gave much more satisfactory ultrastructural preservation and immunoreactive localization of immunoreactive material. This investigation demonstrated that, after combined fixation with PLP and PLP-osmium, we could determine the function of a given cell type in various endocrine organs as well as the precise antigenic sites in such cells.
D000818:Animals; D003594:Cytoplasmic Granules / Q000378:metabolism; D005260:Female; D005399:Fishes / Q000378:metabolism*; D013006:Growth Hormone / Q000378:metabolism*; D006651:Histocytochemistry; D007120:Immunochemistry; D008297:Male; D008854:Microscopy, Electron; D010902:Pituitary Gland / Q000378:metabolism*; D012474:Salmon / Q000378:metabolism*; D014018:Tissue Distribution
D013006:Growth Hormone