title: Selenium-dependent and non-selenium-dependent glutathione peroxidase in human tissues of New Zealand residents.
abstract: Glutathione peroxidase was assayed in human tissues of New Zealand residents by the coupled assay method. Total glutathione peroxidase was assayed using cumene hydroperoxide. The non-selenium-dependent activity was not detected with t-butyl hydroperoxide and thus was determined from the difference between total activity and the selenium-dependent activity using hydrogen peroxide or t-butyl hydroperoxide. Only selenium-dependent activity was found in whole blood, erythrocytes, platelets and biopsy skeletal muscle. A small non-selenium dependent activity was measured in plasma and a larger activity in biopsy liver supernatant and homogenate. Glutathione-S-transferase was detected in all tissues.
mesh_terms: D001555:Benzene Derivatives; D005979:Glutathione Peroxidase / Q000378:metabolism*; D006801:Humans; D009520:New Zealand; D010545:Peroxides; D012643:Selenium / Q000378:metabolism*; D013379:Substrate Specificity; D014018:Tissue Distribution; D020122:tert-Butylhydroperoxide
chemical_list: D001555:Benzene Derivatives; D010545:Peroxides; D020122:tert-Butylhydroperoxide; D005979:Glutathione Peroxidase; D012643:Selenium; C007164:cumene hydroperoxide