Brown adipose tissue from fetal rhesus monkey (Macaca mulatta): morphological and biochemical aspects.
Brown adipose tissue (BAT) from fetal rhesus monkeys microscopically resembled adult rodent BAT containing multiocular fat cells with numerous mitochondria. Mitochondrial carnitine palmitoyl transferase activity was lower than that in adult rodents and adenine nucleotide translocase activity was similar to that reported for rats. Rhesus monkey BAT mitochondria (BATM) possess an uncoupling protein that is characteristic of BAT as evidenced by the binding of [3H]GDP, the inhibition by GDP of the high Cl- permeability or rapid alpha-glycerol-3-phosphate oxidation. Electrophoretic analysis of BATM showed the presence of a 32,000 mol.wt protein which was enriched by procedures established for the isolation of BATM uncoupling protein.
D002001:Adipose Tissue, Brown / Q000033:anatomy & histology / Q000378:metabolism*; D000818:Animals; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D005333:Fetus / Q000033:anatomy & histology / Q000378:metabolism*; D008253:Macaca mulatta; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats