Diuretic and natriuretic actions of extract prepared from rat erythrocytes.
Freeze-dried preparation of rat erythrocytes was extracted into distilled water and after heat treatment the extract was chromatographed on Sephadex G-25 column. The effluent fractions were pooled, freeze-dried, dissolved in Ringer's solution and bioassayed for diuretic and natriuretic responses in anaesthetized non-diuretic rats. The active fractions potently enhanced urine output (about 9-fold control) and urinary sodium excretion (about 7-fold control). The responses started to develop after a lag period of approximately 10-20 min, peaked between 60-80 min post-injection and then diminished. The augmentation of sodium excretion persisted longer than the increased diuresis. The biological activity of the extract was retained after dialysis in a tube with nominal molecular weight cut-off below 1,000.
D000818:Animals; D002457:Cell Extracts / Q000494:pharmacology*; D002850:Chromatography, Gel; D003956:Dialysis; D004232:Diuretics; D004912:Erythrocytes / Q000032:analysis / Q000502:physiology*; D008297:Male; D009318:Natriuresis / Q000187:drug effects*; D045283:Natriuretic Agents; D011506:Proteins / Q000032:analysis; D051381:Rats; D014020:Tissue Extracts / Q000494:pharmacology*
D002457:Cell Extracts; D004232:Diuretics; D045283:Natriuretic Agents; D011506:Proteins; D014020:Tissue Extracts