title: Sidedness studies of thylakoid phosphatidylglycerol in higher plants.
abstract: The transmembrane distribution of phosphatidylglycerol was determined in thylakoids from barley (Hordeum vulgare), lettuce (Lactuca sativa) and pea (Pisum sativum) chloroplasts. Phospholipase A2 and phospholipase D digestion and chemical-labelling methods were used. Phosphatidylglycerol was preferentially localized in the outer (stromal) leaflet. The proportion of the phospholipid in this leaflet ranged from about 66% in pea to about 75% for barley and lettuce thylakoids. One of the main fatty acids, trans-delta 3-hexadecenoic acid, was exclusively located in the outer leaflet in all three plant types. The data are discussed in relation to suggested roles for phosphatidylglycerol in thylakoid function.
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D001467:Hordeum / Q000378:metabolism; D006868:Hydrolysis; D010715:Phosphatidylglycerols / Q000378:metabolism*; D010944:Plants / Q000378:metabolism; D014018:Tissue Distribution; D014675:Vegetables
chemical_list: D005227:Fatty Acids; D010715:Phosphatidylglycerols