title: Measurement of intracellular calcium ion activity with neutral exchanger ion sensitive microelectrodes.
abstract: Micropipettes filled with the neutral liquid ion exchanger ETH 1001 can be used to make microelectrodes that are sensitive to cytoplasmic levels of Ca2+. They are high resistance electrodes, so that care is required in order to record the low current signal. The electrodes often yield 10-15 mV change between intracellular Ca2+ activities of 10(-6) and 10(-7) M, according to a log relation. The microelectrodes are non-destructive, even in rather small cells, and can be used to monitor Ca2+ changes during experimental interventions.
mesh_terms: D000818:Animals; D002118:Calcium / Q000032:analysis*; D002411:Cation Exchange Resins; D003593:Cytoplasm / Q000032:analysis; D007668:Kidney / Q000032:analysis; D008839:Microelectrodes / Q000592:standards; D009206:Myocardium / Q000032:analysis; D009474:Neurons / Q000032:analysis; D011199:Potentiometry; D012756:Sheep
chemical_list: D002411:Cation Exchange Resins; D002118:Calcium