Site-specific DNA binding by the bacteriophage SP01-encoded type II DNA-binding protein.
The bacteriophage SP01 genome encodes a virus-specific type II DNA-binding protein, TF1. The bacterial proteins of this ubiquitous and evolutionarily conserved class are thought to bind non-specifically to DNA. In contrast, the experiments described here demonstrate that TF1 binds to specific sites in SP01 DNA. Several of these sites have been characterized by DNase I 'footprinting' and four of them have been shown to overlap strong phage promoters for Bacillus subtilis RNA polymerase holoenzyme. We speculate on the possible structural basis of site-selective DNA binding by a protein of this class.
D001435:Bacteriophages / Q000235:genetics*; D001665:Binding Sites; D004279:DNA, Viral / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005814:Genes, Viral; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology
D004279:DNA, Viral; D004268:DNA-Binding Proteins; D014157:Transcription Factors