[Identification of the transforming Ha-ras gene in human melanoma, neuroblastoma and breast cancer cells].
Preparations of DNA isolated from malignant melanoma (Ja heterotransplant), neuroblastoma (L-AN-1 cell line) and breast cancer (SK-BR-3 cell line) induced a high incidence of transformation of recipient NIH 3T3 cells in two consecutive cycles of transfection. Transformed NIH 3T3 cells appeared to be highly tumorigenic in newborn BALB/c mice. The analysis of DNA samples isolated from primary and secondary NIH 3T3 transformants identified Alu-repetitive DNA sequences of human genome as well as additional sequences homologous to ras-family oncogene. Transforming c-Ha-ras I gene was identified for the first time in NIH 3T3 cells transformed by preparations of DNA isolated from human malignant melanoma, neuroblastoma and breast cancer.
D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D002478:Cells, Cultured; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D006801:Humans; D008545:Melanoma / Q000235:genetics*; D051379:Mice; D008819:Mice, Nude; D009447:Neuroblastoma / Q000235:genetics*; D009857:Oncogenes; D012091:Repetitive Sequences, Nucleic Acid; D014162:Transfection; D014170:Transformation, Genetic
D004273:DNA, Neoplasm
Identifikatsiia transformiruiushchego gena Ha-ras v kletkakh melanomy, neĭroblastomy i raka molochnoĭ zhelezy cheloveka.