Effects of vitamins on lipid peroxidation and suppression of DNA synthesis induced by adriamycin in Ehrlich cells.
The effects of various vitamins on lipid peroxidation and the suppression of DNA synthesis induced by adriamycin (ADR) in vitro using Ehrlich ascites carcinoma (EAC) cells were studied. ADR produced a concentration-dependent stimulation of lipid peroxidation in EAC cells. alpha-Tocopherol and coenzyme Q10 inhibited ADR-induced lipid peroxidation to about the same extent and these effects were the greatest for all antioxidants added. The inhibitory effect of riboflavin 2',3',4',5'-tetrabutyrate was greater than that of riboflavin 5'-phosphate. On measuring incorporation of [3H]thymidine into EAC cells, these vitamins did not alter appreciably the magnitude of the ADR-induced suppression of DNA synthesis in EAC cells.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000235:genetics*; D003067:Coenzymes; D004261:DNA Replication / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000494:pharmacology*; D005260:Female; D008054:Lipid Peroxides / Q000378:metabolism*; D051379:Mice; D012256:Riboflavin / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000494:pharmacology; D014810:Vitamin E / Q000494:pharmacology; D014815:Vitamins / Q000494:pharmacology*
D003067:Coenzymes; D008054:Lipid Peroxides; D014815:Vitamins; D014451:Ubiquinone; D014810:Vitamin E; D004317:Doxorubicin; C024989:coenzyme Q10; D012256:Riboflavin; D013936:Thymidine