A rapid naphthol yellow S method for measuring the cellular protein content of anchorage cultures.
A rapid method has been developed for measuring the cellular protein content of mono- and multilayered anchorage cultures. Fixed or air dried cultures are stained for 30 min with 0.2% Naphthol Yellow S (NYS) dissolved in 1% acetic acid. Unbound dye is removed by a series of four 2.5 min washes in 1% acetic acid, and protein-bound dye extracted with 10 mM unbuffered Tris base for spectrophotometric optical density determination at 433 nm. The NYS method exhibited a least-squares correlation coefficient of 0.99997 with the Oyama-Eagle Lowry method.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D005910:Glioma / Q000032:analysis*; D006651:Histocytochemistry; D007700:Kinetics; D009282:Naphthalenesulfonates; D011506:Proteins / Q000032:analysis*; D051381:Rats; D013053:Spectrophotometry
D009282:Naphthalenesulfonates; D011506:Proteins; C009824:naphthol yellow