Assay of human fibrinopeptides by high-performance liquid chromatography.
Fibrinopeptides A, AP, and B, desarginine fibrinopeptide B, and a previously unknown peptide corresponding to B beta 3-14 were resolved within 10 min by an HPLC technique using an isocratic solvent system (22% acetonitrile in 0.1% trifluoroacetic acid) and a 0.46 X 10-cm Spherisorb ODS-2 (3-micron) octadecylsilane column. Fibrinopeptides A and AY eluted in the same peptide peak. The method was used to evaluate a carboxypeptidase which converts fibrinopeptide B into its desarginine form. Fifty percent inhibition of this activity occurred at 1.7 mM epsilon-aminocaproic acid (EACA). At saturating substrate concentrations the rates of total fibrinopeptides A and B release were unaffected by 125 mM EACA, a concentration at which the carboxypeptidase activity is completely inhibited.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D015119:Aminocaproic Acid / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005340:Fibrinogen / Q000032:analysis* / Q000378:metabolism; D005344:Fibrinopeptide A / Q000032:analysis*; D005345:Fibrinopeptide B / Q000032:analysis*; D006801:Humans; D007700:Kinetics; D009994:Osmolar Concentration; D013997:Time Factors
D000596:Amino Acids; D005344:Fibrinopeptide A; D005345:Fibrinopeptide B; D005340:Fibrinogen; D015119:Aminocaproic Acid