[Use of monoclonal antibodies in the diagnosis of fibrinolysis].
Monoclonal antibodies to tissue type plasminogen activator (t-PA), urokinase (u-PA) and alpha-2-antiplasmin were obtained by immunizing Balb C mice with the respective purified antigens and fusion of spleen cells with mouse myeloma cells (NSO). The selected monoclonal antibodies reacted exclusively with the respective antigens used for immunization. 3 out of 7 monoclonal antibodies directed against t-PA inhibited plasminogen activation by t-PA and 6 of 7 interfered with the fibrin potentiating effect. Only one out of 5 monoclonal antibodies directed against u-PA inhibited plasminogen activation by u-PA. None of the monoclonal antibodies directed against t-PA or u-PA inhibited the cleavage of low molecular weight paranitroanilide substrates by the respective plasminogen activators. Likewise, the 3 monoclonal antibodies to alpha-2-antiplasmin did not functionally inhibit alpha-2-antiplasmin. The selected monoclonal antibodies were used to develop sensitive test systems for the respective antigens. The lower detection limit for determination of t-PA was found to be 0.5 ng/ml, for determination of u-PA 0.05 ng/ml, and for determination of alpha-2-antiplasmin 5 ng/ml.
D000818:Animals; D000911:Antibodies, Monoclonal; D005342:Fibrinolysis; D006801:Humans; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D010960:Plasminogen Activators / Q000276:immunology; D013154:Spleen / Q000166:cytology; D014568:Urokinase-Type Plasminogen Activator / Q000276:immunology; D000979:alpha-2-Antiplasmin / Q000276:immunology
D000911:Antibodies, Monoclonal; D000979:alpha-2-Antiplasmin; D010960:Plasminogen Activators; D014568:Urokinase-Type Plasminogen Activator
Anwendung monoklonaler Antikörper in der Fibrinolysediagnostik.