[Verapamil inhibits DNA synthesis in vitro. II. Study of the MCF7 and Jurkat neoplastic cell lines].
Verapamil in cultures of mammary carcinoma MCF7 cells and Jurkat A.L.L. cells decreased the 3H-TdR incorporation. The action of Verapamil is dose-dependent. The inhibition percentage of 3H-TdR incorporation into Jurkat cells by Verapamil was lower than into MCF7 cells. No change of the mitotic inhibition was observed with higher Ca++ concentration in the medium. Modification of the Na++ and K++ content in the medium did not change action of Verapamil; this is in agreement with the independence of neoplastic cells from the culture medium. Our investigation suggests that Verapamil does not work through a block of transmembrane Ca++ flux, but rather through an intracellular interaction.
D001943:Breast Neoplasms; D002118:Calcium / Q000494:pharmacology; D002460:Cell Line; D002478:Cells, Cultured; D004247:DNA / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D009374:Neoplasms, Experimental / Q000473:pathology; D014700:Verapamil / Q000494:pharmacology*
D004247:DNA; D014700:Verapamil; D002118:Calcium
II verapamil inibisce la sintesi del DNA "in vitro". II. Studio delle linee neoplastiche MCF7 e Jurkat.