[Local anesthetics: simultaneous HPLC determination of lidocaine (Xylocaine) and bupivacaine (Marcaine) in human serum].
In order to monitor loco-regional anaesthesia in the upper limbs with a combination of two anaesthetics, the authors participated in a clinical pharmacology study. They describe a method of simultaneous assay of bupivacaine and lignocaine by direct phase HPLC. In particular, the use of heptane sulfonic acid (counter-ion) in the mobile phase allows the elution of the local anaesthetics to be controlled. The criteria of reliability studied enable this technique to be considered for routine use.
D000765:Anesthesia, Conduction; D001132:Arm / Q000601:surgery; D002045:Bupivacaine / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007700:Kinetics; D008012:Lidocaine / Q000097:blood*
D008012:Lidocaine; D002045:Bupivacaine
Anesthésiques locaux: dosage simultané par CLHP de lidocaïne (Xylocaïne) et de bupivacaïne (Marcaïne) dans le sérum humain.