[Aggregation of fragmented chromatin associated with the synthesis of products of its treatment with nuclease].
Isolated cell nuclei were incubated with nucleases followed by extraction of chromatin with a low salt buffer. With an increase of nuclear chromatin degradation with DNAse I or micrococcal nuclease, solubilization of deoxyribonucleoprotein (DNP) by a low salt buffer increases, reaching a maximum upon hydrolysis with 2-4% nuclear DNA and then decreases appreciably after extensive treatment with nucleases. Soluble fragmented chromatin aggregates in the course of treatment with DNAase. I. Addition to gel chromatin preparations of exogenous products of nuclease treatment of isolated nuclei leads to its aggregation. Pretreatment of nuclear chromatin with RNAase prevents solubilization of DNP by low ionic strength solutions. Some experimental data obtained with the use of severe nuclease treatment are discussed; for a correct interpretation of these data the aggregation of fragmented chromatin by products of its nuclease degradation should be taken into consideration.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D002843:Chromatin / Q000032:analysis* / Q000302:isolation & purification; D004247:DNA / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis; D003851:Deoxyribonucleases / Q000494:pharmacology*; D006868:Hydrolysis; D066298:In Vitro Techniques; D008099:Liver / Q000032:analysis*; D008114:Liver Neoplasms, Experimental / Q000032:analysis; D009690:Nucleic Acid Conformation; D011487:Protein Conformation; D051381:Rats; D011919:Rats, Inbred Strains; D012995:Solubility
D002843:Chromatin; D004273:DNA, Neoplasm; D004247:DNA; D003851:Deoxyribonucleases
Agregatsiia fragmentirovannogo khromatina, sviazannaia s poiavleniem produktov ego nukleaznoĭ obrabotki.