Low resolution crystal structure of muconolactone isomerase. A decamer with a 5-fold symmetry axis.
Muconolactone isomerase from Pseudomonas putida crystallizes from sodium sulfate solution in space group P2(1) (a = 65.84 A, b = 105.70 A, c = 77.20 A, beta = 90.5 degrees) with ten 11,000 Mr subunits per asymmetric unit. The 7 A resolution crystal structure was solved by single isomorphous replacement followed by 10-fold symmetry averaging. The decameric enzyme has an uncommon non-crystallographic 5-fold symmetry axis and a large cavity in its center.
D001426:Bacterial Proteins; D019748:Carbon-Carbon Double Bond Isomerases; D003461:Crystallography; D007535:Isomerases; D011108:Polymers; D011549:Pseudomonas / Q000201:enzymology
D001426:Bacterial Proteins; D011108:Polymers; D007535:Isomerases; D019748:Carbon-Carbon Double Bond Isomerases; C100087:muconolactone delta-isomerase