title: The effect of pH on alkaline phosphatase activity in serum of the rat and other species.
abstract: Contrary to other species, optimum pH of alkaline phosphatase (alk.P.) activity was 9.6 in fed male rats of all age groups studies. In 3- or 6-week-old fasted rats, the optimum pH shifted to 9.8-10.0. This shifting of pH did not occur in 11-week-old rats. Heating to 56 degrees C for 10 min shifted the optimum pH in 3-week-old rats to 9.0, in 6-week-old rats to 9.0-9.4, but no change occurred in 11-week-old rats. In other species (mouse, rabbit, and man), the optimum pH was 10.0-10.2, and was not influenced by fasting or heating.
mesh_terms: D000328:Adult; D000375:Aging; D000469:Alkaline Phosphatase / Q000097:blood*; D000818:Animals; D002021:Buffers; D005215:Fasting; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000097:blood; D008274:Magnesium; D015636:Magnesium Chloride; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009439:Neuraminidase; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D013696:Temperature
chemical_list: D002021:Buffers; D007527:Isoenzymes; D015636:Magnesium Chloride; D000469:Alkaline Phosphatase; D009439:Neuraminidase; D008274:Magnesium