Extraction of a murine tumor-specific antigen from cells and plasma membranes using 1-butanol: augmentation of antigen yield by colchicine.
The purpose of this investigation was to examine the ability of single-phase aqueous solutions of 1-butanol to release immunoprotective tumor antigen activity from partially purified plasma membranes of the methylcholanthrene-induced fibrosarcoma, MCA-F. Tumor antigen activity was assessed by s.c. immunization of syngeneic C3H/HeJ mice 10 days prior to supralethal challenge. Although brief incubation of intact MCA-F cells in 2.5% butanol releases potent immunoprotective activity, application of this protocol to plasma membranes did not result in antigen extraction. Modification of the extraction protocol using higher concentrations of butanol and longer extraction times did release measurable tumor antigen activity. However, a significant amount of the membrane-associated activity remained with the insoluble membrane fraction, as demonstrated by the immunoprotective capacity of the extracted membranes. The dramatic difference in the extractability of antigen from intact cells and plasma membranes suggested that membrane architecture may influence antigen release. To investigate this possibility, we extracted with butanol MCA-F cells that had been preincubated in colchicine. Treatment of cells with colchicine significantly potentiated the extraction of tumor antigen activity. Augmentation of antigen yield was also observed when plasma membranes were pretreated with colchicine prior to 2.5% butanol extraction. These results suggest that the tumor-specific transplantation antigen may be directly or indirectly associated with the cytoskeleton underlying the plasma membrane.
D020001:1-Butanol; D000818:Animals; D000951:Antigens, Neoplasm / Q000302:isolation & purification*; D000440:Butanols / Q000494:pharmacology*; D002462:Cell Membrane / Q000276:immunology; D003078:Colchicine / Q000494:pharmacology*; D003599:Cytoskeleton / Q000276:immunology; D005260:Female; D005354:Fibrosarcoma / Q000276:immunology; D006649:Histocompatibility Antigens / Q000302:isolation & purification*; D008748:Methylcholanthrene; D051379:Mice; D008809:Mice, Inbred C3H
D000951:Antigens, Neoplasm; D000440:Butanols; D006649:Histocompatibility Antigens; C029237:tumor-associated transplantation antigen; D008748:Methylcholanthrene; D020001:1-Butanol; D003078:Colchicine