Polyadenylation of a human mitochondrial ribosomal RNA transcript detected by molecular cloning.
We have identified by molecular cloning a polyadenylated RNA transcript in the human leukemia cell line, K562, which is complementary to a portion of the gene-encoding mitochondrial 16S ribosomal RNA (mt 16S rRNA). The cloned portion of the transcript corresponds to positions 2191-2395 of the human mt genome. The clone represents a cDNA copy of an RNA transcript from the H strand and carries an additional poly(A) tail 21 residues long at its 3'-end. Our data provide direct evidence for polyadenylation of some mt 16S rRNA transcripts.
D001483:Base Sequence; D002460:Cell Line; D006801:Humans; D007938:Leukemia; D008928:Mitochondria / Q000502:physiology*; D011061:Poly A / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional; D012335:RNA, Ribosomal / Q000235:genetics*
D012335:RNA, Ribosomal; D011061:Poly A