Rapid purification of HBsAg from human plasma.
A method is described for the rapid isolation of purified HBsAg from infected donor plasma. This method uses polyethylene glycol precipitation and a single isopycnic ultracentrifugation step in which the gradient is formed in situ. It works equally well using a small swingout rotor or a zonal rotor.
D002500:Centrifugation, Isopycnic; D002501:Centrifugation, Zonal; D004797:Enzyme-Linked Immunosorbent Assay; D006514:Hepatitis B Surface Antigens / Q000302:isolation & purification*; D006801:Humans; D010949:Plasma / Q000276:immunology* / Q000382:microbiology; D011092:Polyethylene Glycols; D013048:Specimen Handling
D006514:Hepatitis B Surface Antigens; D011092:Polyethylene Glycols