Fluorometric measurement of glycosylated albumin in human serum.
A simple and rapid fluorometric assay of glycosylated albumin was developed using dansylated phenyl boronic acid (N-(5-dimethyl amino-1-naphthalene sulfonyl)-3-aminobenzene boronic acid). The emission spectrum of the reagent changes upon binding to cis-diols present on glycosylated albumin in serum, allowing quantitation of glycosylated albumin. Results from the new procedure correlated well with those from commercially prepared affinity chromatography (r = 0.85).
D001665:Binding Sites; D001897:Boronic Acids; D002846:Chromatography, Affinity; D003619:Dansyl Compounds; D017127:Glycation End Products, Advanced; D006801:Humans; D012709:Serum Albumin / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D000093423:Glycated Serum Albumin
D001897:Boronic Acids; D003619:Dansyl Compounds; D017127:Glycation End Products, Advanced; D012709:Serum Albumin; C032882:N-dansyl-3-aminobenzeneboronic acid; D000093423:Glycated Serum Albumin