NADH-ferrihemoglobin reductase in avian erythrocytes.
The assay for NADH-ferrihemoglobin reductase (NADH-FR) was optimized for avian blood samples. In this assay the pH optimum for Japanese quail red cell NADH-FR was 5.5, which was close to the enzyme's pI. Enzyme kinetic parameters were determined for quail, chicken and turkey NADH-FR. Preparation of erythrocyte ghost-cells and subsequent fractionation showed that the enzyme was present in the plasma membrane as well as in the nuclear membrane, while Triton X-100 treatment gave a release of enzyme activity from the membrane. In the cytosolar fraction of avian red cells no NADH-FR could be detected.
D000818:Animals; D001717:Birds / Q000097:blood*; D002645:Chickens / Q000097:blood; D003370:Coturnix; D042966:Cytochrome-B(5) Reductase / Q000097:blood*; D004910:Erythrocyte Membrane / Q000201:enzymology; D004912:Erythrocytes / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009247:NADH, NADPH Oxidoreductases / Q000097:blood*; D009685:Nuclear Envelope / Q000201:enzymology; D011784:Quail / Q000097:blood; D014422:Turkeys / Q000097:blood
D009247:NADH, NADPH Oxidoreductases; D042966:Cytochrome-B(5) Reductase