The role of DNA synthesis in peptidoglycan-induced generation of immunoglobulin-secreting cells in mice and humans.
The requirement for DNA synthesis in peptidoglycan (PG)-induced activation of polyclonal antibodies has been studied. Inhibition of DNA synthesis with mitomycin C or hydroxyurea at the initiation of the cultures inhibited generation of over 90% of IgM- and IgG-secreting cells in PG-stimulated mouse splenocytes and of over 99.9% of IgM-, IgG- and IgA-secreting cells in PG-stimulated human peripheral blood lymphocytes. Inhibition of DNA synthesis 2 to 4 days after initiation of PG-stimulated cultures (in both mice and humans) caused an immediate decline in the numbers of Ig-secreting cells. These results demonstrate that the magnitude of PG-induced polyclonal antibody response depends on continued DNA synthesis and proliferation of Ig-secreting cells, and indicate that IgM-, IgG- and IgA-secreting cells in polyclonal activation may be actively cycling cells.
D000328:Adult; D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010457:Peptidoglycan / Q000494:pharmacology*; D013997:Time Factors
D007136:Immunoglobulins; D008070:Lipopolysaccharides; D010457:Peptidoglycan; D004247:DNA