Growth and cytogenetic characteristics of bone marrow colonies from patients with 5q-syndrome.
Early erythroid precursor cells and myeloid progenitor cells (CFU-GM) from four patients with 5q-syndrome were cultured in order to study the in vitro growth patterns and to determine the clonal origin of this hematologic disorder. Cultures of CFU-GM exhibited normal colony growth, while erythroid progenitor cells demonstrated a marked decrease or absence of colony growth. Chromosomal studies indicate the 5q-chromosome is present in both hematopoietic progenitor cells, suggesting that the syndrome is an acquired clonal disease arising from a pluripotent hematopoietic stem cell. Follow-up cytogenetic studies reveal a decrease in the number of normal metaphases. This finding is consistent with reports that emphasize the slowly progressive nature of this malignant stem cell defect.
D000368:Aged; D000740:Anemia / Q000097:blood / Q000235:genetics*; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002905:Chromosomes, Human, 4-5; D003114:Colony-Forming Units Assay; D005260:Female; D006412:Hematopoietic Stem Cells / Q000648:ultrastructure*; D006801:Humans; D008297:Male; D008533:Megakaryocytes / Q000648:ultrastructure; D008875:Middle Aged