Effect of platelet homogenate on in vitro glycosaminoglycans production by dermal fibroblasts from systemic sclerosis patients and normal controls.
Confluent cultures of dermal fibroblasts from the involved skin of systemic sclerosis patients (SF) and a matched skin site of normal controls (NF) were investigated for the synthesis of glycosaminoglycans (GAG) in response to various concentrations of human platelet homogenate (PH). Experiments were carried out in the presence of 1% and 15% human serum (HS). In the absence of PH, GAG synthesis was higher in SF than NF. An increase in GAG synthesis was demonstrated in both SF and NF as the concentration of PH was increased to 200 micrograms/ml of growth medium. The PH-stimulated GAG synthesis occurred in 15% HS treated SF and NF, but there was no GAG synthesis increase in 1% HS-treated NF. The absolute count of GAG synthesis was always greater in SF than NF. The addition of PH in concentrations higher than 200 micrograms/ml led to cell death of both SF and NF. These findings are the first to indicate a difference between SF and NF response to PH.
D001769:Blood; D001792:Blood Platelets; D002457:Cell Extracts / Q000494:pharmacology*; D002478:Cells, Cultured; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006801:Humans; D008297:Male; D012595:Scleroderma, Systemic / Q000378:metabolism*; D012867:Skin / Q000378:metabolism*; D014020:Tissue Extracts / Q000494:pharmacology*
D002457:Cell Extracts; D003470:Culture Media; D006025:Glycosaminoglycans; D014020:Tissue Extracts