Preparation and characterization of antibodies with specificity for the amino-terminal tetrapeptide sequence of the platelet-derived connective tissue activating peptide-III.
Antisera selectively reactive with the N-terminal tetrapeptide sequence of the platelet-derived connective tissue activating peptide-III mitogen were prepared and characterized. Solid phase synthesized Z-Asn-Leu-Ala-Lys(Z)-OH tetrapeptide representing the N-terminus of the mitogen was used as an immunogen after carbodiimide mediated coupling to methylated bovine serum albumin carrier and subsequent removal of Z groups. Anti-tetrapeptide sera demonstrated cross-reactivity to the mitogen but not beta-thromboglobulin, fibroblast growth factor, or epidermal growth factor, and a limited cross-reactivity to parathyroid hormone. The studies indicate that the N-terminal sequence of the mitogen is accessible for binding with antibody and the antitetrapeptide sera provide a reagent for the selective measurement of biologically active mitogen in the presence of structurally similar beta-thrombo-globulin. In addition, computer analysis of amino acid sequences revealed that few proteins contain the Asn-Leu-Ala-Lys sequence and of those that do, many are retroviral proteins or transforming polyproteins.
D000595:Amino Acid Sequence; D000918:Antibody Specificity; D001667:Binding, Competitive; D001792:Blood Platelets / Q000276:immunology; D003429:Cross Reactions; D006801:Humans; D009842:Oligopeptides / Q000276:immunology; D010281:Parathyroid Hormone / Q000276:immunology; D010455:Peptides / Q000276:immunology*
D009842:Oligopeptides; D010281:Parathyroid Hormone; D010455:Peptides; C009862:connective tissue-activating peptide