[Course of the concentration of serum free amino acids as a function of time and the method of preservation].
In order to determine the optimal conditions for storage in the case of deferred serum amino acid assay, the authors studied the effect of storing the sample for four hours at two different temperatures: melting ice and ambient temperature, as well as the effect of storing the amino acids for three months according to four methods of storage; freezing at -20 degrees C and -80 degrees C of deproteinised and non-deproteinised serum. When the analysis was performed within four hours of collection of the specimen, the best conditions are obtained when the sample is kept at the temperature of melting ice. The optimal conservation consists of immediately deproteinising the sample and storing it frozen for 14 days.
D000328:Adult; D000596:Amino Acids / Q000097:blood*; D000613:Aminobutyrates / Q000097:blood; D001120:Arginine / Q000097:blood; D001216:Asparagine / Q000097:blood; D001793:Blood Preservation / Q000379:methods*; D001798:Blood Proteins / Q000032:analysis; D002852:Chromatography, Ion Exchange; D005260:Female; D005615:Freezing; D006801:Humans; D008297:Male; D008715:Methionine / Q000097:blood; D009952:Ornithine / Q000097:blood; D010649:Phenylalanine / Q000097:blood; D013696:Temperature; D013997:Time Factors
D000596:Amino Acids; D000613:Aminobutyrates; D001798:Blood Proteins; D010649:Phenylalanine; D001216:Asparagine; C012223:alpha-aminobutyric acid; D001120:Arginine; D008715:Methionine; D009952:Ornithine
Evolution de la concentration des acides aminés libres du sérum en fonction du temps et du mode de conservation.