[Synergistic activation of rat alveolar macrophages by cepharanthine and OK-432].
We studied the synergistic activation of rat alveolar macrophages (AM) by cepharanthine (Ceph.) and OK-432. Rat AM could be rendered much more tumoricidal against Walker-256 tumors in vitro after the i.v. injection of ceph. (5 mg/Kg) and OK-432 (5 KE/rat) thao of ceph. or OK-432 only. Radioactivity of 14C-acetate-OK-432 trapped in murine lung after the i.v. injection of mixed of ceph. was much higher than of 14C-acetate-OK-432 only. Rat AM could be rendered tumoricidal by incubation in vitro with OK-432, but not with ceph. This finding suggests that if OK-432 is injected intravenously with mixed of with ceph., of OK-432 is trapped much more up by the lung without mixture, therefore AM can be much more tumoricidal. Intravenous administration of OK-432 with ceph. may be useful for reduction of lung metastasis by tumoricidal AM.
D000470:Alkaloids / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D044182:Benzylisoquinolines; D001688:Biological Products / Q000494:pharmacology*; D002279:Carcinoma 256, Walker / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004357:Drug Synergism; D005260:Female; D007275:Injections, Intravenous; D008262:Macrophage Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D010844:Picibanil / Q000008:administration & dosage / Q000494:pharmacology*; D011650:Pulmonary Alveoli / Q000166:cytology*; D051381:Rats; D011919:Rats, Inbred Strains; D052117:Benzodioxoles
D000470:Alkaloids; D044182:Benzylisoquinolines; D001688:Biological Products; D010844:Picibanil; C006947:cepharanthine; D052117:Benzodioxoles