title: Optimisation of conditions for infection of isolated potato protoplasts with potato virus X.
abstract: Conditions for the reproducible infection of potato protoplasts with PVX were determined. Successful virus infection was achieved when potato protoplasts at a concentration of 3 X 10(5) cells/ml were incubated with 20 micrograms/ml PVX and 2 micrograms/ml Poly-L-ornithine (MW 100,000) for 15 minutes at 0 degrees C. Numbers of protoplasts infected varied between 30 and 50 per cent. Preincubation of PVX with PLO for a minimum of 10 minutes was essential for good infection. The type and molecular weight of polycation used had a major effect on virus infection. PLO at 100,000 MW was the most efficient.
mesh_terms: D002470:Cell Survival; D010455:Peptides / Q000494:pharmacology; D010942:Plant Viruses / Q000254:growth & development*; D010944:Plants; D011107:Polylysine / Q000494:pharmacology; D011523:Protoplasts / Q000187:drug effects; D013696:Temperature; D014776:Virus Cultivation
chemical_list: D010455:Peptides; C008973:polyornithine; D011107:Polylysine