High-performance liquid chromatographic determination of d-/l-epinephrine enantiomer ratio in lidocaine-epinephrine local anesthetics.
A procedure for the determination of the ratio of d- to l-epinephrine in lidocaine-epinephrine local anesthetics is described. Epinephrine was isolated via Sep-Pak cartridges and derivatized with 2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosyl isothiocyanate; the d- and l-chiral derivatives were separated and measured by high-performance liquid chromatography. About 70 samples of various dosage forms and concentrations from four manufacturers were successfully analyzed by the method.
D000766:Anesthesia, Dental; D000779:Anesthetics, Local / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004338:Drug Combinations; D004837:Epinephrine / Q000032:analysis*; D007202:Indicators and Reagents; D008012:Lidocaine / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D012965:Sodium Chloride / Q000032:analysis; D013237:Stereoisomerism; D013447:Sulfites
D000779:Anesthetics, Local; D004338:Drug Combinations; D007202:Indicators and Reagents; D013447:Sulfites; D012965:Sodium Chloride; D008012:Lidocaine; C009279:sodium bisulfite; D004837:Epinephrine