title: Identification of thiamine diphosphate-binding proteins from rat liver supernatant.
abstract: Transketolase (EC 2.2.1.1) was shown to be the sole enzyme protein bound to thiamine diphosphate (ThDP) as coenzyme in the soluble fraction of rat liver. No new ThDP-binding proteins with the molar ratio of ThDP to protein of 1.0 were detected in the rat liver supernatant under the purification and assay conditions employed.
mesh_terms: D000818:Animals; D002250:Carbon Radioisotopes; D003600:Cytosol / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D013835:Thiamine Pyrophosphate / Q000378:metabolism*; D014174:Transketolase / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D002250:Carbon Radioisotopes; D014174:Transketolase; D013835:Thiamine Pyrophosphate