The recovery of nitrocellulose-bound protein.
Conditions for the electroelution of protein from polyacrylamide gels to nitrocellulose and its subsequent recovery have been examined. A procedure is described whereby soluble material suitable for further analysis can be obtained. Nitrocellulose-bound protein is dissolved in acetone. It can be precipitated from solution in the presence of a carrier such as Polybrene in a form that can subsequently be solubilized in good yield.
D000096:Acetone; D000199:Actins / Q000302:isolation & purification; D000818:Animals; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006583:Hexadimethrine Bromide; D008970:Molecular Weight; D011506:Proteins / Q000302:isolation & purification*; D012997:Solvents; D014404:Tubulin / Q000302:isolation & purification
D000199:Actins; D011506:Proteins; D012997:Solvents; D014404:Tubulin; D000096:Acetone; D006583:Hexadimethrine Bromide; D003101:Collodion