[Physico-chemical properties of lysyl-tRNA-synthetase from the rat liver].
Two lysyl-tRNA-synthetase forms are obtained from the rat liver. Their molecular masses are determined by electrophoresis and gel-filtration on Sephadex G-150: form I-122, form II-64 kDalton. Gel-electrophoresis in the presence of 0.1% SDS indicates that form I of lysyl-tRNA-synthetase consists of two subunits with a molecular mass of 64 kDalton each, i. e. it is a dimer. Optimal conditions and kinetic parameters (Km and Vmax) of aminoacylation for the both enzyme forms are similar. Amino acid composition, fluorescence parameters and thermal inactivation conditions are determined.
D000595:Amino Acid Sequence; D000604:Amino Acyl-tRNA Synthetases / Q000032:analysis*; D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008250:Lysine-tRNA Ligase / Q000032:analysis* / Q000302:isolation & purification; D008970:Molecular Weight; D011487:Protein Conformation; D051381:Rats; D013379:Substrate Specificity
D000604:Amino Acyl-tRNA Synthetases; D008250:Lysine-tRNA Ligase
Fiziko-khimicheskie svoĭstva lizil-tRNK-sintetazy pecheni krys.