Antibody-directed urokinase: a specific fibrinolytic agent.
A specific fibrinolytic agent was synthesized by covalently coupling urokinase to a monoclonal antibody that was fibrin-specific and did not cross-react with fibrinogen. The antibody was raised against a synthetic peptide representing the seven amino-terminal residues of the beta chain of human fibrin. The urokinase-antifibrin conjugate retained the original binding specificity of the antibody and showed 100-fold increased fibrinolysis in vitro when compared to unmodified urokinase. The presence of human fibrinogen at plasma concentration did not influence these properties.
D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D003432:Cross-Linking Reagents; D005337:Fibrin / Q000276:immunology; D005342:Fibrinolysis; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D013329:Structure-Activity Relationship; D014568:Urokinase-Type Plasminogen Activator / Q000008:administration & dosage*
D000911:Antibodies, Monoclonal; D003432:Cross-Linking Reagents; D005337:Fibrin; D014568:Urokinase-Type Plasminogen Activator