Proteins transferred to nitrocellulose for use as immunogens.
Proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and were eluted efficiently from the gel by transfer to nitrocellulose paper. The protein-bearing nitrocellulose was solubilized with dimethyl sulfoxide and used as an immunogen in rabbits. Polyclonal antibodies to the proteins were generated.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002642:Chick Embryo; D002846:Chromatography, Affinity; D003101:Collodion; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D007118:Immunoassay / Q000379:methods*; D011506:Proteins / Q000276:immunology / Q000302:isolation & purification*; D011817:Rabbits
D000911:Antibodies, Monoclonal; D011506:Proteins; D003101:Collodion; D004121:Dimethyl Sulfoxide