Nuclease-hypersensitive sites in chromatin of the estrogen-inducible apoVLDL II gene of chicken.
DNAseI-hypersensitive sites were localized in apoVLDL II chromatin from chicken. In the liver two sites at 1.75 and 1.0 kb upstream from the cap-site are present before the gene is activated. After induction by estradiol a number of additional sites appear, three in the promotor region of the gene, one within the coding region and two behind the poly-A signal. These sites disappear when the expression of the gene is shut off upon estradiol withdrawal. All sites appear to be tissue-specific in that they are not found in other tissues of the rooster. However, in oviduct of the laying hen we find a hypersensitive site at 1.6 kb in front of the gene.
D000818:Animals; D001053:Apolipoproteins / Q000235:genetics*; D001483:Base Sequence; D002645:Chickens; D002843:Chromatin / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism*; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D008079:Lipoproteins, VLDL / Q000235:genetics*; D008099:Liver / Q000032:analysis; D008297:Male; D010057:Oviducts / Q000032:analysis
D001053:Apolipoproteins; D002843:Chromatin; D004967:Estrogens; D008079:Lipoproteins, VLDL; C027610:apolipoprotein VLDL II; D003850:Deoxyribonuclease I