Induction of cytochrome P-450 isozymes in liver microsomes of rats treated with the cardiotonic agent sulmazole.
Pretreatment of adult male rats with sulmazole (AR-L 115 BS) results in a 2-6-fold increase of the liver microsomal scoparone O-demethylation and 7-ethoxycoumarin O-deethylation activity. The change in the ratio of the demethylation products scopoletin to isoscopoletin from 1 : 1.8 +/- 0.1 in control microsomes to 1: 2.5 +/- 0.1 in microsomes from sulmazole pretreated rats is statistically significant. Sulmazole produces a modified type II difference spectrum when added to microsomes of control or sulmazole-pretreated rats. Immunoquantitation of seven cytochromes P-450 showed that two forms, namely P-450 beta NF/ISF-G and P-450 beta NF-B, are increased 3-4-fold in the microsomes of sulmazole-pretreated rats.
D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis* / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D007093:Imidazoles / Q000494:pharmacology*; D007122:Immunoelectrophoresis; D007527:Isoenzymes / Q000096:biosynthesis* / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012603:Scopoletin / Q000378:metabolism
D002316:Cardiotonic Agents; D007093:Imidazoles; D007527:Isoenzymes; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; C029758:sulmazole; D012603:Scopoletin