Molecular parameters and conformation of globoside and asialo-GM1.
Comparative studies of the individual properties and intermolecular organization of asialo-GM1 (Gg4Cer) and globoside (Gb4Cer) were made employing lipid monolayers and high-sensitivity differential scanning calorimetry. The surface pressure-area isotherm of Gb4Cer is more expanded than that of Gg4Cer. This results in greater molecular areas and compressibilities, and lower intermolecular interaction energies, for Gb4Cer compared to Gg4Cer at all surface pressures. This looser intermolecular packing may be responsible for a lower transition temperature (40.5 degrees C) and enthalpy of transition (delta Hcal) (2.0 kcal mol-1) found for the phase transition of Gb4Cer compared to Gg4Cer (54.0 degrees C and 4.2 kcal mol-1). The surface potential per molecule and resultant molecular dipole moment vector values are greater for Gb4Cer than for Gg4Cer at comparable values of surface pressure and molecular area. All these results reflect the existence of a rigid L-shape in the oligosaccharide chain of Gb4Cer that prevents a close intermolecular packing compared to the straight orientation of the polar head group of Gg4Cer. Significant movements of the oligosaccharide chain may occur depending on the lateral surface pressure. At low surface pressures the orientation of the oligosaccharide chain of Gg4Cer may be displaced an angle of up to about 40 degrees compared to the perpendicular position adopted at high surface pressures. In agreement with an enhanced liquid character of the interface, the oligosaccharide chain of Gb4Cer exhibits a greater freedom of movement and the displacement from the position perpendicular to the interface can reach to about 65 degrees.
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005677:G(M1) Ganglioside; D005915:Globosides; D006028:Glycosphingolipids; D008563:Membrane Lipids; D008958:Models, Molecular; D008968:Molecular Conformation; D012995:Solubility; D013499:Surface Properties; D013696:Temperature; D013816:Thermodynamics
D005915:Globosides; D006028:Glycosphingolipids; D008563:Membrane Lipids; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside