title: Analysis of the fragile-X chromosome: localization and detection of the fragile site in high resolution preparations.
abstract: Fragile X chromosome preparations were analyzed at levels of up to 850 bands per haploid set. We were able to consistently sublocalize the Xqter fragile site to band Xq27.3 using high-resolution methods. Chromosome length versus the frequency of fragile X expression was also compared. The fragile site appeared at a higher percentage in more condensed chromosome preparations. The importance of this finding is that high-resolution chromosome preparations do not optimize fragile-X detection.
mesh_terms: D002871:Chromosome Banding; D043283:Chromosome Fragile Sites; D002873:Chromosome Fragility; D005260:Female; D006801:Humans; D007621:Karyotyping; D008297:Male; D014960:X Chromosome