Ethanol toxicity in primary cultures of rat myocardial cells.
The potential cardiotoxicity of ethanol (EtOH) was evaluated in primary cultures of rat myocardial cells. EtOH cardiotoxicity was assessed in the cells on the basis of cell morphology, lactate dehydrogenase (LDH) leakage, succinate dehydrogenase (SDH) activity, and beating rates. Cells were treated with EtOH at concentrations of 600, 800, and 1000 mg% for duration of 1, 4, and 24 h and then evaluated for cardiotoxicity. Vacuole formation occurred 1 h after exposure to EtOH at 800 and 1000 mg%; by 4 h, cytosolic granular material appeared in these cells. Exposure for 24 h to all concentrations of EtOH resulted in vacuole, granule, and pseudopod formation and loss of cross-striations. Significant LDH leakage occurred at 1 h and 4 h with 800 and 1000 mg% EtOH. LDH release was significantly increased after 24 h with all concentrations. SDH activity was significantly depressed after 24 h with all concentrations of EtOH. Beating rates were altered as early as 1 h after exposure to 800 and 1000 mg% EtOH. After 24 h, those cells exposed to the highest concentrations of EtOH were not beating at all. These data suggest that primary myocardial cell cultures may be used to assess the in vitro cardiotoxicity of EtOH to the myocardial cell.
D000818:Animals; D002478:Cells, Cultured; D003594:Cytoplasmic Granules / Q000187:drug effects; D000431:Ethanol / Q000633:toxicity*; D006321:Heart / Q000187:drug effects*; D006339:Heart Rate / Q000187:drug effects; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000201:enzymology / Q000473:pathology / Q000648:ultrastructure; D011554:Pseudopodia / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013385:Succinate Dehydrogenase / Q000378:metabolism; D014617:Vacuoles / Q000187:drug effects
D000431:Ethanol; D007770:L-Lactate Dehydrogenase; D013385:Succinate Dehydrogenase