Human mononuclear phagocyte differentiation: a study of the U-937 cell line by ultrastructural cytochemistry and surface antigen analysis.
U-937 represents a well-established permanent human haematopoietic cell line, which exhibits characteristics of the monocyte/macrophage series. U-937 cells were investigated by peroxidase ultrastructural cytochemistry in order to determine the normal developmental stage to which they correspond. This study was performed in non- and TPA-stimulated cells, in conjunction with surface analysis by monoclonal antibodies. It is concluded: (1) peroxidase-positive U-937 cells are monoblasts and promonocytes involved in myeloperoxidase synthesis; (2) TPA-stimulation caricatures transformation of these cells into monocytes but not into resident macrophages, as far as peroxidase cytochemistry is concerned; (3) the reactivity of myeloperoxidase present in the endoplasmic reticulum of synthesizing cells is inhibited by glutaraldehyde fixation.
D000954:Antigens, Surface / Q000032:analysis; D002454:Cell Differentiation; D002460:Cell Line; D006412:Hematopoietic Stem Cells / Q000201:enzymology / Q000276:immunology / Q000648:ultrastructure*; D006651:Histocytochemistry; D006801:Humans; D008264:Macrophages / Q000648:ultrastructure*; D008854:Microscopy, Electron; D009000:Monocytes / Q000648:ultrastructure*; D010544:Peroxidases / Q000378:metabolism
D000954:Antigens, Surface; D010544:Peroxidases