title: Sensitivity and specificity of salamandrid integumental transepithelial potential to prolactin.
abstract: The effects of ovine prolactin (oPRL), bovine growth hormone (bGH) and human placental lactogen (hPL) on in vivo integumental transepithelial potential (TEP) were examined in two salamandrid urodeles, adult terrestrial-phase Taricha granulosa and the juvenile red-eft stage of Notophthalmus viridescens. TEP in efts treated with 1.0 microgram oPRL/2 days fell from 73.3 +/- 6.1 to 15.2 +/- 5.5 mV by Day 7 (P less than 0.001), whereas the TEP of efts treated with either 1.0 or 10 micrograms bGH/2 days remained at control levels for as long as 24 days. TEP in efts treated with a single dose of 10 micrograms oPRL dropped from 65.74 +/- 4.1 to 23.0 +/- 3.4 mV (P less than 0.01) in 3 days. Efts treated with various doses of oPRL showed a linear log total dose response over the range of 0.05 to 10.0 micrograms oPRL/animal, with a minimum detectable total dose of 0.4 micrograms/g (0.01 IU/g). In the same experiments, tail height increased by Day 7 in efts treated every other day with 10.0 micrograms oPRL, but not 1.0 microgram oPRL or either 1.0 or 10.0 micrograms bGH/2 days. In Oregon newts injected every other day with 10 micrograms oPRL, TEP decreased by 33% in 8 days (P less than 0.05), whereas in animals treated with 10 micrograms bGH/2 days, TEP did not change from control values even after 23 days. TEP in Oregon newts receiving a single dose of 100 micrograms oPRL dropped to 68% of initial values within 2 days (P less than 0.05), but subsequently recovered to control values 3 weeks after the last injection.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D004594:Electrophysiology; D004848:Epithelium / Q000187:drug effects; D009673:Notophthalmus viridescens; D011388:Prolactin / Q000494:pharmacology*; D012455:Salamandridae / Q000502:physiology*; D012867:Skin / Q000187:drug effects*; D013997:Time Factors
chemical_list: D011388:Prolactin