The calcium-dependent myoblast adhesion that precedes cell fusion is mediated by glycoproteins.
Presumptive myoblasts from explants of chick embryo pectoral muscle proliferate, differentiate, and fuse to form multinucleate myotubes. One event critical to multinucleate cell formation is the specific adhesion of myoblasts before union of their membranes. In the studies reported here five known inhibitors of myotube formation--trifluoperazine, sodium butyrate, chloroquine, 1,10 phenanthroline, and tunicamycin--were tested for their effect on the Ca++-dependent myoblast adhesion step. The first four inhibitors of myotube formation do not perturb myoblast adhesion but rather block fusion of aggregated cells, which suggests that these agents perturb molecular events required for the union of the lipid bilayers. By contrast, tunicamycin exerts its effect by inhibiting the myoblast adhesion step, thereby blocking myotube formation. The effect of tunicamycin can be blocked by a protease inhibitor, however, which implies that the carbohydrate residues protect the glycoproteins from proteolytic degradation rather than participate directly in cell-cell adhesion. Whereas trypsin treatment of myoblasts in the absence of Ca++ destroys the cells' ability to exhibit Ca++-dependent adhesion, the presence of Ca++ during trypsin treatment inhibits the enzyme's effect, which suggests that myoblast adhesion is mediated by a glycoprotein(s) that has a conformation affected by Ca++. Finally, myoblast adhesion is inhibited by an antiserum raised against fusion-competent myoblasts. The effect of the antiserum is blocked by a fraction from the detergent extract of pectoral muscle that binds to immobilized wheat germ agglutinin, which again suggests that glycoproteins mediate Ca++-dependent myoblast adhesion.
D000818:Animals; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002118:Calcium / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation; D002459:Cell Fusion / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D002738:Chloroquine / Q000494:pharmacology; D006023:Glycoproteins / Q000502:physiology*; D009124:Muscle Proteins / Q000502:physiology*; D009132:Muscles / Q000166:cytology* / Q000196:embryology; D010618:Phenanthrolines / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D014268:Trifluoperazine / Q000494:pharmacology; D014415:Tunicamycin / Q000494:pharmacology
D002087:Butyrates; D006023:Glycoproteins; D009124:Muscle Proteins; D010618:Phenanthrolines; D011480:Protease Inhibitors; D020148:Butyric Acid; D014415:Tunicamycin; D014268:Trifluoperazine; D002738:Chloroquine; D002118:Calcium