Preservation of the human cochlea.
Human cochleas processed with a varying interval between death and initial fixation were examined by transmission and scanning electron microscopy. The specimens were fixed by paraformaldehyde-glutaraldehyde fixatives in buffer. Cochleas fixed less than two hours postmortem showed excellent morphology in general. With increasing time lag between death and fixation the results became more variable. In some specimens fixed up to six hours postmortem, however, the morphology still was well preserved, but as the interval increased, a greater variability was introduced. The conclusion is that specimens fixed up to six hours postmortem can show excellent electron microscopic morphology. Limited information can be obtained up to 12 hours postmortem, after which the results tend to be unreliable.
D000328:Adult; D000368:Aged; D003051:Cochlea / Q000648:ultrastructure; D005404:Fixatives; D006801:Humans; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D009926:Organ Preservation / Q000379:methods*; D013997:Time Factors
D005404:Fixatives