Isolation, amino acid sequence, synthesis, and biological activity of some oligopeptides from porcine spinal cord.
Four oligopeptides, designated SCP-3, SCP-4, SCP-5, and SCP-6, have been isolated and purified to homogeneity from porcine spinal cord. The amino acid sequences have been determined as pyroGlu-Gly, pyroGlu-Gly-Gly, Met-Met-Gly, and Asp-Ala-Gly-Ala-Gly, respectively. All of these peptides have been synthesized by conventional liquid-phase or solid-phase methods. The synthetic and extracted peptides showed identical behavior in a reverse-phase high-performance liquid chromatography system. SCP-3 and SCP-4 exhibited some significant inhibitory activity on the electrical stimulation-induced contractions of longitudinal muscle strip of guinea pig ileum, and SCP-5 showed some stimulating effect on the same preparation. The physiological significance of these purified peptides is being investigated.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000302:isolation & purification*; D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004558:Electric Stimulation; D006168:Guinea Pigs; D007082:Ileum / Q000502:physiology; D066298:In Vitro Techniques; D009119:Muscle Contraction; D009130:Muscle, Smooth / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000502:physiology; D009842:Oligopeptides / Q000096:biosynthesis* / Q000302:isolation & purification / Q000502:physiology; D013116:Spinal Cord / Q000378:metabolism*; D013552:Swine
D000596:Amino Acids; D009419:Nerve Tissue Proteins; D009842:Oligopeptides