Comparative physiological disposition of some anthraquinone glycosides and aglycones.
The in vitro microbial degradation and the urinary excretion and biliary secretion in rats of two anthraquinone glycosides (sennosides A and B) and four aglycones (sennidins A and B, rhein, and danthron) were studied using a high performance liquid chromatographic system with gradient elution and amperometric detection. Microbial degradation of sennosides A and B occurred almost exclusively in the presence of mice caecum inoculae and was associated with the release of sennidins A and B. Rhein and danthron were indiscriminately metabolized by bacteria sampled from all regions of mice intestine, whereas sennidins lacked stability in biological media. The fraction of the dose administered orally to rats and recovered as aglycones or as glucuronides in bile and urine after 48 hours was five times greater for rhein (15 per cent) and danthron (13.4 per cent) than for sennosides A (1.8 per cent) and B (2.8 per cent) excreted or secreted as sennidins. These results support the concept that anthraquinone glycosides are less likely to enter the systemic circulation and, thus, are able to exert their laxative effect at lower doses than aglycones.
D000818:Animals; D000880:Anthraquinones / Q000378:metabolism* / Q000652:urine; D001419:Bacteria / Q000378:metabolism; D001646:Bile / Q000378:metabolism; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D006027:Glycosides / Q000378:metabolism; D007422:Intestines / Q000382:microbiology; D007700:Kinetics; D008297:Male; D051379:Mice; D051381:Rats; D011919:Rats, Inbred Strains; D012676:Senna Extract; D000081226:Sennosides
D000880:Anthraquinones; D006027:Glycosides; D000081226:Sennosides; D012676:Senna Extract; C020491:rhein; C004315:danthron