[Structural characteristics and availability of DNA for solvent molecules in nuclei of Physarum polycephalum].
The rate constants of 1H--3H exchange between water and C8H-groups of purinic residues of DNA in solution and in nuclei of synchronous Physarum polycephalum at different phases of the cell cycle were measured. The nuclear membranes and nonhistone proteins were shown not to create additional hindrances and not to diminish the availability of DNA in nuclei for solvent molecules. A small increase of the rate of the 1H--3H exchange between water and the DNA of nuclei upon transition from the S-phase to the late G2-phase seems to reflect the process of chromatin decondensation connected with activation of the transcription and local changes of the secondary structure of DNA at the late G2-phase of the cell cycle.
D002453:Cell Cycle; D004271:DNA, Fungal / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D010804:Physarum / Q000166:cytology / Q000378:metabolism*; D011685:Purine Nucleotides / Q000378:metabolism; D012997:Solvents; D014316:Tritium / Q000378:metabolism
D004271:DNA, Fungal; D011685:Purine Nucleotides; D012997:Solvents; D014316:Tritium
Strukturnaia kharakteristika i dostupnost' DNK dlia molekul rastvoritelia v iadrakh Physarum polycephalum.