Isolation of intact sperm asters from fertilized sea urchin eggs.
We have developed a procedure for isolating intact sperm asters in quantity from fertilized sea urchin eggs. This procedure is based on detergent-extraction methods developed previously for the bulk isolation of mitotic apparatuses. Using this protocol it is possible to isolate sperm asters as soon as they appear in the fertilized egg or at any subsequent point in their brief existence.
D000818:Animals; D005260:Female; D008297:Male; D008870:Microtubules / Q000648:ultrastructure; D011506:Proteins / Q000378:metabolism; D012617:Sea Urchins / Q000648:ultrastructure*; D013094:Spermatozoa / Q000378:metabolism / Q000648:ultrastructure*; D013997:Time Factors; D015053:Zygote / Q000648:ultrastructure*
D011506:Proteins