title: Determination of polysorbate in ascites fluid from a premature infant.
abstract: A method is described for the determination of poly(oxy-1,2-ethanediyl)oligomers in body fluids using ammonium cobaltothiocyanate complexation in conjunction with high pressure liquid chromatography and visible spectrophotometry. Analysis of peritoneal fluid from a baby given E-ferol, a vitamin E supplement, revealed levels as high as 100 micrograms/mL polysorbate.
mesh_terms: D001202:Ascitic Fluid / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003124:Colorimetry / Q000379:methods; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D011136:Polysorbates / Q000032:analysis* / Q000378:metabolism; D014810:Vitamin E / Q000008:administration & dosage
chemical_list: D011136:Polysorbates; D014810:Vitamin E