title: mRNA activity of a Sindbis virus defective-interfering RNA.
abstract: We obtained Sindbis defective-interfering particles by nine and undiluted passages of standard virus on chicken embryo fibroblasts. These particles contain a deleted 20S RNA molecule which has mRNA activity, as shown by translation in cell-free systems in vitro. In infected cells, this mRNA activity appeared to be totally inhibited except at very late times postinfection.
mesh_terms: D003673:Defective Viruses / Q000235:genetics; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism; D012845:Sindbis Virus / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral