Rapid quantitative method for simultaneous determination of benzoic acid, sorbic acid, and four parabens in meat and nonmeat products by liquid chromatography.
A liquid chromatographic (LC) method for the simultaneous determinations of benzoic acid, sorbic acid, and methyl, ethyl, propyl, and butyl parabens (methyl, ethyl, propyl, and butyl-p-hydroxybenzoates) in meat and nonmeat products was developed. Benzoic acid, sorbic acid, and parabens were extracted from meat and nonmeat products with 70% ethanol. After filtration, extracts were analyzed by reverse phase liquid chromatography. Homogeneously ground samples of fresh sausage and hamburger were fortified with benzoic acid, sorbic acid, and each paraben at 5 different concentrations. Average recovery (after discarding outliers) for each preservative at all 5 levels was greater than 95% with a coefficient of variation less than 5%.
D000818:Animals; D001565:Benzoates / Q000032:analysis*; D019817:Benzoic Acid; D002417:Cattle; D002853:Chromatography, Liquid; D005231:Fatty Acids, Unsaturated / Q000032:analysis*; D007202:Indicators and Reagents; D008460:Meat / Q000032:analysis*; D008461:Meat Products / Q000032:analysis*; D010226:Parabens / Q000032:analysis*; D013011:Sorbic Acid / Q000032:analysis*; D013552:Swine
D001565:Benzoates; D005231:Fatty Acids, Unsaturated; D007202:Indicators and Reagents; D010226:Parabens; D019817:Benzoic Acid; D013011:Sorbic Acid