Reduction of 7-ketolithocholic acid to chenodeoxycholic acid by rat liver preparations in vitro.
The formation of chenodeoxycholic acid via 7-ketolithocholic acid by rat liver preparations was examined in vitro. Results showed that a rat liver preparation reduced 7-ketolithocholic acid mainly to chenodeoxycholic acid and to ursodeoxycholic acid in a smaller amount, and that the reductase required NADPH but not NADH as coenzyme and was mainly localized in the microsomes.
D000818:Animals; D002635:Chenodeoxycholic Acid / Q000096:biosynthesis*; D066298:In Vitro Techniques; D007700:Kinetics; D008095:Lithocholic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains
D002635:Chenodeoxycholic Acid; C023020:7-ketolithocholic acid; D009249:NADP; D008095:Lithocholic Acid