An investigation of a mouse model to estimate the potency of the diphtheria component in vaccines.
A mouse model to estimate the potency of the diphtheria toxoid components in vaccines using Vero cells to detect the neutralizing antibodies in the sera from immunized mice is described. The results obtained with this mouse model correlated significantly with those obtained in the lethal challenge test in guinea-pigs. For this reason it is suggested that the potency test in guinea-pigs be replaced by this mouse model because a considerable reduction in the number of animals used and costs can be achieved by the introduction of this mouse test for the routine control of the potency of the diphtheria component of vaccines.
D000818:Animals; D001681:Biological Assay / Q000379:methods; D004166:Diphtheria Antitoxin / Q000276:immunology; D004168:Diphtheria Toxoid / Q000032:analysis / Q000276:immunology* / Q000592:standards; D005069:Evaluation Studies as Topic; D005260:Female; D006168:Guinea Pigs; D007928:Lethal Dose 50; D008297:Male; D051379:Mice
D004166:Diphtheria Antitoxin; D004168:Diphtheria Toxoid