In vitro IgE-secreting cells in man. I. Mitogen-independent and -dependent subpopulations.
In vitro IgE secretion by atopic and normal peripheral-blood lymphocytes was examined in culture with pokeweed mitogen or Staphylococcus aureus strain Cowan-I (StaCw) or without mitogen. IgE secreted in culture supernatants was measured with double antibody radioimmunoassay. Enumeration of IgE-secreting cells was made by a protein-A plaque assay. IgE was detected in increasing quantities in supernatants of cultured lymphocytes without mitogen up to the 12th day. IgE-plaque-forming cells were formed by the lymphocytes in large numbers on days 4-7 in cultures with mitogen. These results suggest that not only mitogen-independent but also mitogen-dependent subpopulations may exist in the IgE-secreting cells.
D000921:Antibody-Producing Cells / Q000187:drug effects / Q000276:immunology* / Q000528:radiation effects; D002648:Child; D003561:Cytarabine / Q000494:pharmacology; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology; D007073:Immunoglobulin E / Q000096:biosynthesis*; D066298:In Vitro Techniques; D007700:Kinetics; D008214:Lymphocytes / Q000276:immunology; D008934:Mitogens / Q000494:pharmacology*
D008934:Mitogens; D003561:Cytarabine; D007073:Immunoglobulin E