Toxicity of commercial products on cells in suspension culture: a possible screen for the Draize eye irritation test.
Incubation of LS cells in suspension for 4 hr in the presence of water-soluble commercial products with known Draize rabbit-eye irritation potential has shown that viability, as indicated by fluorescent dyes, is a credible screen for irritation. For product formulations containing immiscible and/or insoluble substances, there are difficulties that have been reduced by extraction of the soluble components of the formulations into the culture medium. A more sensitive and automated, though more expensive, technique based on the estimation of intracellular ATP levels has been developed and the ATP50 values (concentration that reduces intracellular ATP by 50%) for several substances indicate that this may be another suitable endpoint in cytotoxicity screening for animal irritation studies.
D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005123:Eye / Q000187:drug effects*; D007509:Irritants / Q000633:toxicity*; D051379:Mice; D013535:Suspensions
D007509:Irritants; D013535:Suspensions; D000255:Adenosine Triphosphate