title: Lectin binding to glycoproteins in the surface membrane of Schistosoma mansoni.
abstract: A number of lectins were assessed for their ability to bind to glycoproteins in the surface membrane of Schistosoma mansoni. The membrane polypeptides were separated by SDS-PAGE and the glycoproteins visualised by incubating the gel with radio-iodinated lectin followed by autoradiography. Most of the individual lectins bound to a variety of glycoproteins but peanut agglutinin and Dolichos biflorus agglutinin bound preferentially to a single glycoprotein of apparent molecular weight 170 000. This glycoprotein was subsequently shown to be exposed at the surface of the parasite and localised at the tubercles.
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000032:analysis / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis / Q000378:metabolism*; D037102:Lectins; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism*; D010455:Peptides / Q000032:analysis; D037121:Plant Lectins; D011975:Receptors, Mitogen / Q000032:analysis; D012550:Schistosoma mansoni / Q000032:analysis / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D037102:Lectins; D008565:Membrane Proteins; D010455:Peptides; D037121:Plant Lectins; D011975:Receptors, Mitogen; C036554:dolichos biflorus agglutinin; C038223:peanut agglutinin receptor