Direct enzyme transfer from lymphocytes corrects a lysosomal storage disease.
Fibroblasts from patients with mannosidosis, the lysosomal storage disease resulting from an inherited deficiency of lysosomal alpha-D-mannosidase (EC 3.2.1.24), accumulate specific mannose-containing oligosaccharides which are characteristic of the disease (1,2). The present study shows that these substances were extensively degraded following transfer of the missing enzyme from normal lymphocytes to mannosidosis fibroblasts on direct contact in tissue culture. Moreover, prolonged correction of the metabolic abnormality of the recipient cells was sustained if contact with fresh donor lymphocytes was periodically renewed. These findings may be highly relevant to lymphocyte function in enzyme replacement therapy by transplantation procedures currently being attempted.
D000818:Animals; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism; D008361:Mannosidases / Q000378:metabolism* / Q000627:therapeutic use; D051379:Mice; D009844:Oligosaccharides / Q000378:metabolism; D043323:alpha-Mannosidase; D008363:alpha-Mannosidosis / Q000188:drug therapy / Q000378:metabolism*
D009844:Oligosaccharides; D008361:Mannosidases; D043323:alpha-Mannosidase; D008358:Mannose