Oil-stop method as an alternative to filtration for transport studies on plasma membrane vesicles. A preliminary report.
The oil-stop procedure applied in transport studies on whole cells was adapted for isolated plasma membrane vesicles. The advantages of the proposed technique with respect to the commonly used filtration method are the reduction of the period of the termination step, the increase in the allowed membrane content of the samples and the absence of the leakage of substrates from the intravesicular space.
D000818:Animals; D001692:Biological Transport; D002458:Cell Fractionation / Q000379:methods*; D002462:Cell Membrane / Q000378:metabolism*; D003993:Dibutyl Phthalate; D004051:Diethylhexyl Phthalate; D007940:Leukemia L5178 / Q000378:metabolism; D051379:Mice; D013936:Thymidine / Q000378:metabolism; D014462:Ultrafiltration
D003993:Dibutyl Phthalate; D004051:Diethylhexyl Phthalate; D013936:Thymidine