title: Optical model studies of the salt-induced 10-30-nm fiber transition in chromatin.
abstract: Fractionated chicken erythrocyte chromatin fibers consisting of 10-mer and 75-mer polynucleosomes have been studied by flow birefringence and viscosity over a range of Na+ and Mg2+ ion concentrations sufficient to span the 10-30-nm fiber transition. Negative intrinsic flow bifringence was observed under all solvent conditions investigated. The intrinsic birefringence, obtained from the reduced birefringence to intrinsic viscosity ratio, was used to evaluate various optical models for the DNA conformation in the fiber. Results are consistent with an extended chromatosome-linker "necklace" model for the unfolded, low-salt fiber and with a solenoidal model of edge-stacked chromatosomes for the condensed fiber at high salts. These results are consistent with and independently corroborative of similar models based upon electric dichroism and neutron scattering reported by others.
mesh_terms: D000818:Animals; D001718:Birefringence; D002645:Chickens; D002843:Chromatin / Q000187:drug effects / Q000648:ultrastructure*; D004247:DNA / Q000097:blood; D004912:Erythrocytes / Q000648:ultrastructure; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000648:ultrastructure; D009994:Osmolar Concentration; D012964:Sodium / Q000494:pharmacology; D014783:Viscosity
chemical_list: D002843:Chromatin; D009707:Nucleosomes; D004247:DNA; D012964:Sodium; D008274:Magnesium