Biogenic aldehydes in brain: characteristics of a reaction between rat brain tissue and indole-3-acetaldehyde.
When indole-3-acetaldehyde was incubated with rat brain tissue, an aldehyde dehydrogenase-independent irreversible disappearance of the aldehyde was found. This was accompanied by an increase in absorbance at 240-400 nm, with a peak at 310 nm. The results suggested that this change in absorbance was caused by a membrane-bound nonenzymatic reaction between indole-3-acetaldehyde and phospholipids. A similar reaction occurred between indole-3-acetaldehyde and pure preparations of phosphatidylethanolamine and phosphatidylserine, but not phosphatidylcholine. Indole-3-acetaldehyde levels also decreased slightly when incubated with albumin but absorbance at 310 nm was unaltered. It is suggested that nonenzymatic reactions between indole-3-acetaldehyde (or other biogenic aldehydes) and membrane components might occur in vivo, and could be involved in the effects of drugs such as ethanol and barbiturates.
D000418:Albumins / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000187:drug effects*; D001923:Brain Chemistry / Q000187:drug effects; D005260:Female; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D007210:Indoleacetic Acids / Q000032:analysis; D007211:Indoles / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008995:Monoamine Oxidase / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013347:Subcellular Fractions / Q000032:analysis / Q000187:drug effects
D000418:Albumins; D007210:Indoleacetic Acids; D007211:Indoles; D010743:Phospholipids; D009243:NAD; C001655:indole-3-acetaldehyde; D012701:Serotonin; C030737:indoleacetic acid; D007770:L-Lactate Dehydrogenase; D005969:Glutamate Dehydrogenase; D008995:Monoamine Oxidase