Hydrolase activity in acute otitis media with effusion.
Biochemical studies of middle ear effusions (MEE) from patients with chronic or recurrent otitis media with effusion (OME) have demonstrated the presence of significant levels of certain hydrolytic and oxidative enzymes. We have examined MEE from patients with acute OME for the content of a number of lysosomal hydrolases and find no significant differences in the mean values for acid phosphatase, alpha-mannosidase, beta-galactosidase, beta-glucuronidase, hexosaminidase, and neuraminidase between purulent and serous effusions. In every case, the mean activities of these enzymes were greater in culture-positive than in culture-negative effusions although this difference was significant only in the case of neuraminidase. Neuraminidase activity was detected in 78% of those MEEs from which Streptococcus pneumoniae could be cultured and in only 32% to 64% of all other effusions. No correlation was observed between the level of neuraminidase released into the extracellular growth medium and the infectivity of various strains of S pneumoniae.
D000208:Acute Disease; D002648:Child; D002675:Child, Preschool; D005122:Exudates and Transudates / Q000201:enzymology / Q000382:microbiology; D005260:Female; D006801:Humans; D006867:Hydrolases / Q000378:metabolism*; D007223:Infant; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D009439:Neuraminidase / Q000378:metabolism; D010033:Otitis Media / Q000201:enzymology*; D010034:Otitis Media with Effusion / Q000201:enzymology* / Q000382:microbiology; D010035:Otitis Media, Suppurative / Q000201:enzymology / Q000382:microbiology
D006867:Hydrolases; D009439:Neuraminidase