The ovarian enzyme catalyzing conversion of 13,14-dihydro-15-ketoprostaglandin F2 alpha to 13,14-dihydroprostaglandin F2 alpha in rats.
The preliminary experiments concerning enzymes involved in the production system of 13,14-dihydroprostaglandin F2 alpha (13,14H2-PGF2 alpha) in rat ovary were carried out. The enzyme which converted 13,14-dihydro-15-keto-prostaglandin F2 alpha (15KD-PGF2 alpha) to 13,14H2-PGF2 alpha required NADPH as a cofactor, and the enzyme activity was inhibited by sulfhydryl reagents as well as quercitrin. Thus, the enzyme studied here seems to be a carbonyl reductase possessing SH group(s) in the molecule.
D000818:Animals; D015237:Dinoprost / Q000031:analogs & derivatives*; D005260:Female; D010053:Ovary / Q000201:enzymology*; D011460:Prostaglandins F / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013439:Sulfhydryl Reagents / Q000494:pharmacology
D011460:Prostaglandins F; D013439:Sulfhydryl Reagents; C026527:13,14-dihydroprostaglandin F2alpha; C008228:15-keto-13,14-dihydroprostaglandin F2alpha; D015237:Dinoprost