Trichostrongylus colubriformis: in vitro culture of parasitic stages and their use for the evaluation of anthelmintics.
Approximately 50 per cent of fourth stage larvae of Trichostrongylus colubriformis taken from the gerbil, Meriones unguiculatus, on day 8 after infection, moulted to the young adult stage when cultured in a complex medium over a seven day period in vitro. Larvae at the late fourth stage of development were highly susceptible to certain benzimidazole, prebenzimidazole, imidazothiazole, pyrimidine, quaternary ammonium, organophosphorus and macrocyclic lactone anthelmintics when any of these were included at very low concentrations in the culture medium. However, few anthelmintics lacking activity against T colubriformis in vivo affected these larvae. An assay employing these larvae in vitro should offer a means for assessing the intrinsic activity of new compounds against T colubriformis in the absence of any complicating host pharmacokinetic factors, and could also be adapted for use as a high capacity preliminary screen. Thus it should now be possible to employ a target parasite at the earliest stages of a lead discovery programme obviating the need to use less relevant free-living nematodes or ones that are natural parasites of rodents.
D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D003470:Culture Media; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005849:Gerbillinae / Q000469:parasitology*; D007814:Larva; D014251:Trichostrongyloidea / Q000187:drug effects / Q000254:growth & development*
D000871:Anthelmintics; D003470:Culture Media