A simple method for purification of rat hepatic microsomal cytochrome b5.
Rat hepatic microsomal cytochrome b5 was purified to homogeneity by solubilization with the detergent Lubrol 12-A9 and chromatography on Fractogel TSK DEAE-650(S). The protein was obtained in high yield (52-87%) in 8 h, and only one polypeptide band, of Mr 16 600, was visible after sodium dodecyl sulphate/polyacrylamide-gel electrophoresis.
D000818:Animals; D002850:Chromatography, Gel / Q000379:methods*; D003573:Cytochrome b Group / Q000302:isolation & purification*; D015786:Cytochromes b5; D004591:Electrophoresis, Polyacrylamide Gel; D008862:Microsomes, Liver / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013053:Spectrophotometry
D003573:Cytochrome b Group; D015786:Cytochromes b5