The amino acid sequence of protein CM-3 from Dendroaspis polylepis polylepis (black mamba) venom.
Protein CM-3 from Dendroaspis polylepis polylepis venom was purified by gel filtration and ion exchange chromatography. It comprises 65 amino acids including eight half-cystines. The complete amino acid sequence of protein CM-3 has been elucidated. The sequence (residues 1-50) resembles that of the N-terminal sequence of the subunits of a synergistic type protein and residues 51-65 that of the C-terminal sequence of an angusticeps type protein. Mixtures of protein CM-3 and angusticeps type proteins showed no apparent synergistic effect, in that their toxicity in combination was no greater than the sum of their individual toxicities.
D000595:Amino Acid Sequence; D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002918:Chymotrypsin; D004546:Elapid Venoms / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000032:analysis; D012911:Snakes; D014357:Trypsin
D004546:Elapid Venoms; D010446:Peptide Fragments; C046523:black mamba venom protein CM 3; D002918:Chymotrypsin; D014357:Trypsin