Synthesis, storage, and stability of [4-14C]oxaloacetic acid.
A simple procedure for preparing [4-14C]oxaloacetic acid based on the reaction between [14C]HCO-3 and phosphoenolpyruvate catalyzed by phosphoenolpyruvate carboxylase is described. A simple method for preparing highly purified phosphoenolpyruvate carboxylase from maize leaves is described and the degradation of oxaloacetate under conditions of varying pH and divalent metal ion concentration is reported on. [4-14C]Oxaloacetic acid is stable for several months in 0.1 M HCl solution at -80 degrees C.
D002250:Carbon Radioisotopes; D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D002852:Chromatography, Ion Exchange; D003300:Copper; D019327:Copper Sulfate; D007553:Isotope Labeling; D010071:Oxaloacetates / Q000138:chemical synthesis*; D010730:Phosphoenolpyruvate Carboxylase / Q000302:isolation & purification; D013048:Specimen Handling; D013997:Time Factors; D003313:Zea mays / Q000201:enzymology
D002250:Carbon Radioisotopes; D010071:Oxaloacetates; D003300:Copper; D010730:Phosphoenolpyruvate Carboxylase; D019327:Copper Sulfate