Evidence of an active glucose uptake in Rhizobium meliloti.
Rhizobium meliloti M5N1 showed immediate uptake of glucose. Glucose accumulation was a saturable function of the glucose concentration, and km and Vmax values for uptake were determined. The glucose uptake system was found to be proteic with a turnover of about 6 h. This system was observed to be an active ATP-dependent process, since it was severely inhibited by uncouplers. Glucose analogues and others hexoses had some inhibitory effects on glucose uptake, but not polyols and lactose.
D000255:Adenosine Triphosphate / Q000378:metabolism; D001667:Binding, Competitive; D001693:Biological Transport, Active / Q000187:drug effects; D050260:Carbohydrate Metabolism; D002352:Carrier Proteins / Q000378:metabolism; D003470:Culture Media; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D009004:Monosaccharide Transport Proteins; D012231:Rhizobium / Q000254:growth & development / Q000378:metabolism*
D002352:Carrier Proteins; D003470:Culture Media; D009004:Monosaccharide Transport Proteins; D000255:Adenosine Triphosphate; D005947:Glucose