Influence of dietary cis- and trans-fat on 1,2-dimethylhydrazine-induced colon tumors and fecal steroid excretion in Fischer 344 rats.
To investigate the effects of the difference in the geometry of dietary fatty acids on colon tumorigenesis, male rats were fed semipurified diets containing either partially hydrogenated corn oil (trans-monoene fat) or olive oil (cis-monoene fat) at the 10% level and received a single oral dosage of 1,2-dimethylhydrazine (DMH). The difference in the fatty acid composition of dietary fats was confined essentially to the geometrical isomerism of octadecenoate, and the linoleic acid content was made equivalent (2% of total energy). After about 15 mo of feeding, colon tumor incidence in DMH treated rats was nearly the same in both fat groups. Fecal neutral steroid excretion was higher, while the transformation of cholesterol to coprostanol was lower in rats given the trans-fat. There were no marked differences in the excretion and composition of fecal bile acids between two fat groups. Serum cholesterol and tocopherol levels of rats given trans-fat diets tended to be low. The results suggested that the trans-monoene behaves much like the cis-monoene in the incidence of DMH-induced colon tumors, although there were characteristic differences in metabolic events in the intestine.
D019813:1,2-Dimethylhydrazine; D000818:Animals; D002784:Cholesterol / Q000097:blood; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000473:pathology*; D004041:Dietary Fats / Q000494:pharmacology*; D004127:Dimethylhydrazines; D005227:Fatty Acids / Q000032:analysis; D005243:Feces / Q000032:analysis*; D008055:Lipids / Q000032:analysis; D008099:Liver / Q000032:analysis; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D013256:Steroids / Q000378:metabolism*; D014801:Vitamin A / Q000097:blood; D014810:Vitamin E / Q000097:blood
D004041:Dietary Fats; D004127:Dimethylhydrazines; D005227:Fatty Acids; D008055:Lipids; D013256:Steroids; D014801:Vitamin A; D014810:Vitamin E; D002784:Cholesterol; D019813:1,2-Dimethylhydrazine