Hemocyanins in spiders, XX. Sulfhydryl groups and disulfide bridges in subunit d of Eurypelma californicum hemocyanin.
Subunit d of Eurypelma californicum hemocyanin contains after reduction 7 cysteine residues. Using 3,3'-dithiobis(6-nitrobenzoic acid) 3 mol cysteine/mol subunit were determined. The cysteine- and cystine-containing peptides of subunit d were obtained by cyanogen bromide cleavage and subsequent treatment with trypsin. The free cysteines were established at positions 102, 261, and 454 respectively. Cys205-Cys210 and Cys529-Cys579 are connected by disulfide bridges.
D000595:Amino Acid Sequence; D000818:Animals; D003545:Cysteine; D004220:Disulfides; D006433:Hemocyanins; D010446:Peptide Fragments / Q000302:isolation & purification; D011487:Protein Conformation; D013112:Spiders / Q000032:analysis*
D004220:Disulfides; D010446:Peptide Fragments; D006433:Hemocyanins; D003545:Cysteine