Tabulation and assessment of 113 human surveillance cytogenetic studies conducted between 1965 and 1984.
There is an increasing tendency to monitor human exposure to genotoxic chemicals by the assessment of chromosomal aberrations or sister-chromatid exchanges (SCEs) in peripheral blood lymphocytes. In order to assess the sensitivity of these techniques, and to discern minimal criteria for their conduct, a survey of 113 human lymphocyte cytogenetic surveillance studies conducted between 1965 and 1984 has been undertaken. The present survey indicates the urgent need for standardization of study protocols. It is suggested that a common method of reporting chromosomal aberrations should be adopted, and that this should be based on the system described by Scott et al. It is also suggested that a minimum acceptable size of control and exposed populations should be agreed, and that potentially important factors such as the gender, the period and extent of exposure and individual smoking habits be defined in advance of the commission of future surveillance studies. As general awareness of the possible hazard presented by exposure of man to genotoxic chemicals increases, so appropriate preventative industrial hygiene measures will be instituted. This implies that future human cytogenetic surveillance studies may yield either weakly positive or negative data. This emphasizes the current need for agreement on appropriate study protocols. The formation of a central repository for control databases, and its subsequent updating and use by those involved in human cytogenetic surveillance studies, is recommended. Minimal experimental criteria for the design of future studies are also outlined.
D002274:Carcinogens, Environmental / Q000494:pharmacology; D002869:Chromosome Aberrations; D003582:Cytogenetics; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000494:pharmacology; D004812:Epidemiologic Methods; D014943:Global Health; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000494:pharmacology; D009369:Neoplasms / Q000139:chemically induced; D009784:Occupational Diseases / Q000139:chemically induced / Q000453:epidemiology / Q000235:genetics*; D011159:Population Surveillance; D012107:Research Design; D012854:Sister Chromatid Exchange / Q000187:drug effects / Q000528:radiation effects; D012907:Smoking
D002274:Carcinogens, Environmental; D004785:Environmental Pollutants; D009153:Mutagens