Protein-binding and urinary excretion of 99mTc(Sn)-MDP and 99mTc-MDP.
In an incubation experiment the Human Serum Albumin (HSA) binding of 99mTc-MDP (electrolytically labeled) and 99mTc(Sn)-MDP is established. During the incubation some pertechnetate is formed and in the case of 99mTc(Sn)-MDP also some hydrolyzed 99mTc. The HSA binding of 99mTc-MDP is less than the HSA binding of 99mTc(Sn)-MDP as established with gel chromatography, TCA-precipitation, ammonium sulfate precipitation and ultrafiltration. TCA-precipitation seems to be an insufficient method for determining the protein binding of 99mTc(Sn)-MDP. The urinary excretion in rats shows only one 99mTc-compound in both cases. The bone seeking properties of the urine-excreted 99mTc-compound were confirmed in another rat.
D000818:Animals; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D006801:Humans; D007700:Kinetics; D008297:Male; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000378:metabolism*; D013669:Technetium Tc 99m Medronate / Q000378:metabolism* / Q000652:urine
D012709:Serum Albumin; C023568:technetium Tc 99m (Sn)methylenediphosphonate; D013669:Technetium Tc 99m Medronate