Liquid chromatographic determination of carbadox residues in animal feed.
A liquid chromatographic (LC) method for determining residues of carbadox in the 0.01-10 ppm range in swine feed is described. Carbadox is extracted from ground feed with 25% acidified methanol-CHCl3, removed from emulsion-forming coextractables via an alumina column, separated from highly colored pigments by acid-base liquid-liquid partitioning, and finally isolated from interferences on a second alumina column. Isocratic reverse phase LC at 305 nm is used for quantitation. The average overall recovery at the 0.1, 0.5, and 1.0 ppm spike levels was 83.0% with a standard deviation of 2.04% and a coefficient of variation of 2.46%.
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002218:Carbadox / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002853:Chromatography, Liquid; D011810:Quinoxalines / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D013552:Swine
D011810:Quinoxalines; D002218:Carbadox