Flow cytometric studies on modulation of cellular adriamycin retention by phenothiazines.
Phenothiazines (chlorpromazine and trifluoperazine) enhance the cellular retention and cytotoxicity of adriamycin in P388/S and P388/R cells. Effects on the resistant cells are more pronounced than those on the sensitive cells. Multiparametric flow-cytometric determination of cellular Adriamycin fluorescence shows that the phenothiazine effects are not uniform in a cell population, and cells in later part of the cell cycle traverse (S and G2/M), mitogen-stimulated lymphocytes (as compared to nonstimulated lymphocytes), and ascites (as compared to splenocytes) have greater sensitivity to phenothiazine effects on Adriamycin retention.
D000818:Animals; D002147:Calmodulin / Q000502:physiology; D002453:Cell Cycle; D002460:Cell Line; D004317:Doxorubicin / Q000378:metabolism* / Q000494:pharmacology; D005434:Flow Cytometry; D005470:Fluorometry; D006801:Humans; D007941:Leukemia P388 / Q000378:metabolism; D008213:Lymphocyte Activation; D010640:Phenothiazines / Q000494:pharmacology*; D014410:Tumor Stem Cell Assay
D002147:Calmodulin; D010640:Phenothiazines; D004317:Doxorubicin