[Immunoenzyme analysis of the antigenic orientation of "anti-brain" autoantibodies in the serum of epileptics].
With the help of a solid-phase ELISA and the test system of rat brain antigens (protein fractions divided by isoelectrofocusing into zones differing in pI values) the authors discovered autoantibodies to species-nonspecific protein antigens of the brain in the blood sera of epileptic patients and obtained data on the spectra of the antigenic orientation of these autoantibodies. It has been noted that the epileptics' blood sera are characterized by high titres of autoantibodies to glial brain-specific proteins of the group S-100, as well as by undifferentiated proteins, with pI values 3-3.5 and 5-5.5. There were no significant differences in the spectra of the antigenic orientation of autoantibodies in patients with different frequencies of seizures and the localization of the epileptic focus. Considerable differences in the orientation of cerebral autoantibodies in patients with different disease standing were discovered.
D000818:Animals; D001323:Autoantibodies / Q000032:analysis*; D001921:Brain / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D004827:Epilepsy / Q000097:blood / Q000276:immunology*; D006801:Humans; D007525:Isoelectric Focusing; D051381:Rats; D013045:Species Specificity
D001323:Autoantibodies
Immunofermentnyĭ analiz antigennoĭ napravlennosti "antimozgovykh" avtoantitel syvorotki krovi bol'nykh épilepsieĭ.