title: Rat brain synthesizes two 'vitamin D-dependent' calcium-binding proteins.
abstract: Two proteins from rat brain reacting against anti-chick intestinal vitamin D-dependent calcium-binding protein were characterized in terms of their mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and their molecular size. The proteins were present in the isolated cytoplasm and were produced following translation of brain mRNA in the rabbit reticulocyte lysate system. Their apparent molecular weight was 29,000 and 27,000 daltons whereas rat kidney contained only one protein cross-reacting with this antiserum and with a molecular weight of 27,000 daltons.
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000096:biosynthesis*; D002474:Cell-Free System; D002531:Cerebellum / Q000032:analysis / Q000378:metabolism*; D066298:In Vitro Techniques; D008970:Molecular Weight; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D064030:S100 Calcium Binding Protein G / Q000096:biosynthesis* / Q000302:isolation & purification
chemical_list: D002135:Calcium-Binding Proteins; D012333:RNA, Messenger; D064030:S100 Calcium Binding Protein G