title: Analysis of chickens for recombination within the MHC (B-complex).
abstract: In an attempt to further map the chicken MHC (the B complex), a systematic search for genetic recombinants within the B complex was performed by serotyping the progeny from F2 crosses of chickens by means of specific anti-class I, anti-class II, and anti-class IV alloantisera. Two recombinant B-haplotypes (B21r and B15r) were found by analysing 2,656 F2 chickens representing 5,312 informative typings. In either case, the B-G (class IV) allele was recombined with both the B-F and B-L alleles of the opposite haplotype. MLC typings, tests for direct compatibility by GVH reactions, and absorption analyses confirmed the original serological typing of the two recombinant B haplotypes. No recombination between B-F (class I) and B-L (class II) loci was found. This very low frequency of recombination within the B complex as compared with recombination frequencies found in mammalian MHC's is discussed.
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics* / Q000276:immunology; D002874:Chromosome Mapping; D003434:Crossing Over, Genetic; D006649:Histocompatibility Antigens / Q000276:immunology; D008285:Major Histocompatibility Complex; D011995:Recombination, Genetic
chemical_list: D006649:Histocompatibility Antigens