Heart cells in culture: a model of myocardial iron overload and chelation.
The effect of iron loading and chelation was studied in heart cell cultures obtained from newborn rats. Radioactive iron uptake per 2 X 10(6) cells/24 hr was 3.8% for 59Fe-transferrin, 15.8% for 59Fe-ferric ammonium citrate (FeAC) at 20 micrograms Fe/ml in 20% serum, and 37.1% for 59FeAC at 20 micrograms Fe/ml in serum-free medium. About one third of the cellular radioactive iron was in ferritin and the rest in an insoluble lysosomal fraction. Iron uptake was almost completely inhibited by reducing the incubation temperature from 37 degrees C to 10 degrees C. Intracellular concentrations of malonyldialdehyde (MDA) were doubled after 15 minutes of iron loading and reached maximal concentrations at 3 hours. Conversely, iron mobilization by deferoxamine at concentrations ranging from 0.025 mmol/L to 0.3 mmol/L resulted in normalization of cellular MDA concentrations, in direct proportion to the amounts of iron removed. These findings indicate that cultured myocardial cells are able to assimilate large amounts of nontransferrin iron and that iron uptake and mobilization are associated with striking changes in lipid peroxidation as manifested by the respective increase and decrease in cellular MDA concentrations.
D000818:Animals; D002478:Cells, Cultured; D002614:Chelating Agents / Q000494:pharmacology* / Q000627:therapeutic use; D003676:Deferoxamine / Q000494:pharmacology; D005293:Ferritins / Q000378:metabolism; D006331:Heart Diseases / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism*; D006432:Hemochromatosis / Q000188:drug therapy / Q000378:metabolism*; D007501:Iron / Q000378:metabolism / Q000506:poisoning*; D007504:Iron Radioisotopes; D008054:Lipid Peroxides / Q000096:biosynthesis; D008315:Malondialdehyde / Q000096:biosynthesis; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013696:Temperature; D014168:Transferrin / Q000378:metabolism
D002614:Chelating Agents; D007504:Iron Radioisotopes; D008054:Lipid Peroxides; D014168:Transferrin; D008315:Malondialdehyde; D005293:Ferritins; D007501:Iron; D003676:Deferoxamine