Isolation of polysomes from permissive and non-permissive invertebrate cell lines infected with chilo iridescent virus.
Chilo Iridescent virus (Iridovirus type 6 or CIV) infection results in a disaggregation of the heavy polyribosomes both in permissive and non-permissive invertebrate cell lines. The integrity of the viral genome is not involved in this event, as shown by the polysome absorbance profile on sucrose gradient, prepared from cells infected with UV treated virus. Heavy polysomes reappeared in permissive infected cells during the viral replication cycle.
D000330:Aedes; D000818:Animals; D002458:Cell Fractionation; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D005814:Genes, Viral; D007497:Iridoviridae / Q000378:metabolism / Q000502:physiology*; D007915:Lepidoptera; D011132:Polyribosomes / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins