Methionine synthase assay based on coupling with thymidylate synthase reaction.
Methionine synthase reaction may be coupled with thymidylate synthase-catalysed tritium release from [5-3H]dUMP via non-enzymatic reaction of formaldehyde with tetrahydrofolate. A convenient and sensitive assay of methionine synthase activity, based on this principle, is described.
D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / Q000032:analysis*; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008780:Methyltransferases / Q000032:analysis*; D051379:Mice; D013940:Thymidylate Synthase / Q000032:analysis*
D008780:Methyltransferases; D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase; D013940:Thymidylate Synthase