title: A rapid method for the determination of blood morphine concentration suitable for use in studies involving acute and chronic pain.
abstract: This communication provides an improved sample preparation method for blood morphine concentration determination prior to high-performance liquid chromatography analysis with electrochemical detection. The lower limit of sensitivity is 1 ng/ml of whole blood, and the standard curve is linear to an upper limit of 500 ng/ml. The coefficient of variation of replicate determinations of morphine at concentrations of 10 and 400 ng/ml were 4.7 and 2.5%, respectively. This assay procedure is rapid and accurate and allows the large number of samples (usually 30-40 samples) generated by pharmacokinetic studies in acute and chronic pain to be conveniently analyzed in one day.
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002908:Chronic Disease; D005260:Female; D006801:Humans; D008297:Male; D009020:Morphine / Q000097:blood*; D009369:Neoplasms / Q000503:physiopathology; D010146:Pain / Q000188:drug therapy*; D010149:Postoperative Pain / Q000188:drug therapy
chemical_list: D009020:Morphine