Neonucleoproteins. Preparation and high-performance liquid chromatographic characterization of succinyl-lysozyme-diaminooctyl-polycytidylic acid and related polycytidylic acid conjugates.
Transamination conjugates of cytidine-3'-phosphate and polycytidylic acid (poly C) with diaminopropane, diaminohexane and diaminooctane (DAO) are formed both at 25 degrees C and 60 degrees C. The extent of reaction and formation of side products, with intermittent hydrolysis to mononucleotides in the case of aminoalkyl-poly C, is monitored by reversed-phase high-performance liquid chromatography. Both Dns-DAO-poly C and succinyl-lysozyme-DAO-poly C covalent conjugates are then prepared and similarly characterized, including separation on a size-exclusion diol high-performance liquid chromatography column. The retention of the latter on a wide-pore reversed-phase column seems to be controlled by the protein moiety.
D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D003619:Dansyl Compounds / Q000138:chemical synthesis; D007700:Kinetics; D009113:Muramidase; D011066:Poly C / Q000138:chemical synthesis* / Q000302:isolation & purification; D011131:Polyribonucleotides / Q000138:chemical synthesis*; D013696:Temperature
D003619:Dansyl Compounds; D011131:Polyribonucleotides; D011066:Poly C; D009113:Muramidase