[Effects of calcium influx blocker on the cellular transport of Adriamycin].
Effects of the calcium influx blocker, verapamil, on the cellular transport of adriamycin (ADR) were studied using the technique of flow cytometry. A marked increase in intracellular ADR accumulation was brought about by verapamil treatment. Flow cytometric analysis suggested that this augmentation of cellular ADR content was due to the inhibition of active ADR efflux from the cells. This inhibitory effect of verapamil was dependent on the concentration of the calcium influx blocker in the culture medium.
D000818:Animals; D001692:Biological Transport; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002460:Cell Line; D003470:Culture Media; D004317:Doxorubicin / Q000378:metabolism*; D005434:Flow Cytometry; D066298:In Vitro Techniques; D007940:Leukemia L5178 / Q000378:metabolism* / Q000473:pathology; D007942:Leukemia, Experimental / Q000378:metabolism*; D014700:Verapamil / Q000494:pharmacology*
D002121:Calcium Channel Blockers; D003470:Culture Media; D004317:Doxorubicin; D014700:Verapamil