In vitro dopamine biosynthesis in the median eminence of rat hypothalamic slices: possible involvement of a Na+-Ca2+ exchange mechanism.
Equimolar replacement of Na+ in medium with choline chloride or sucrose and experimental manipulations known to increase [Na+]i, such as ouabain addition and K+ deprivation from medium, caused a marked increase in in vitro DOPA synthesis in the median eminence of rat hypothalamic slices in a Ca2+-dependent manner. These results suggest that a Na+-Ca2+ exchange mechanism is closely involved in the regulation of dopamine biosynthesis in tuberoinfundibular neurons.
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002369:Castration; D002463:Cell Membrane Permeability; D004298:Dopamine / Q000096:biosynthesis*; D005260:Female; D066298:In Vitro Techniques; D008473:Median Eminence / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000378:metabolism*
D012964:Sodium; D002118:Calcium; D004298:Dopamine