The non-lethal effects of ionizing radiation and hyperthermia on colony growth in a human bladder cancer cell line.
The doses of radiation and 43 degrees C hyperthermia required to produce 90% killing of a human bladder cancer cell line were determined using a classical colony assay. The growth rate of survivors was then described by carrying out colony-size analysis at sequential times after treatment. It was demonstrated that those cells which initially survive radiation frequently show sustained growth delay and that their progeny may stop growing after a few generations of cell division. After an 'equilethal' dose of hyperthermia there is transient growth delay but there is no long term loss of reproductive integrity in the survivors.
D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects*; D006801:Humans; D006979:Hyperthermia, Induced; D014411:Neoplastic Stem Cells / Q000528:radiation effects; D001749:Urinary Bladder Neoplasms / Q000473:pathology*