Corrected relative net protein ratio (CRNPR) method based on differences in rat and human requirements for sulfur amino acids.
The requirement for sulfur amino acids was calculated for growing rats fed 8% protein diets. The rat and human requirements for sulfur amino acids were compared and a correction factor was developed to reflect the differences. This correction factor was used to determine corrected relative net protein ratio (CRNPR) values for a number of potential meat extenders and their mixtures with beef. The methionine + cystine requirement of growing rats was estimated to be 4% of protein for 8% protein diets. The methionine + cystine requirement of rats was about 50% higher than that of humans (2.65% of protein). Based on this comparison (rat/human), a correction factor of 1.5 was developed to correct RNPR values of those protein products that were deficient in sulfur amino acids for rat growth. The CRNPR values of beef, casein, soybean protein products, pea concentrate, and peanut meal were 100, 100, 91-97, 75, and 73, respectively. Mixtures (50:50 protein basis) of beef with casein, soybean concentrate, soybean isolate, pea concentrate, peanut meal, rapeseed concentrate, rapeseed isolate, sunflower isolate, or wheat gluten were equal to beef in CRNPR values. The CRNPR method is a good predictor of protein quality for humans of those protein products that are deficient in sulfur amino acids.
D000596:Amino Acids / Q000032:analysis; D000603:Amino Acids, Sulfur / Q000032:analysis*; D000818:Animals; D002417:Cattle; D004044:Dietary Proteins / Q000032:analysis*; D006128:Growth / Q000187:drug effects; D008297:Male; D008460:Meat / Q000032:analysis; D009751:Nutritional Requirements; D051381:Rats; D011919:Rats, Inbred Strains; D013025:Glycine max / Q000032:analysis
D000596:Amino Acids; D000603:Amino Acids, Sulfur; D004044:Dietary Proteins