Histochemical localization of hormone sensitive adenylate cyclase in defined nephron epithelia in culture.
Distal nephron epithelia of defined anatomical origin were microdissected from rabbit kidneys and individually explanted into an in vitro culture system. The 7 day monolayers grown from four different nephron epithelia were studied for the presence and amount of adenylate cyclase reaction product. In each case basal adenylate cyclase was compared with the enzyme reaction product after stimulation by arginine vasopressin, calcitonin, parathyroid hormone (PTH) and isoproterenol. In cortical collecting tubule cultures, the reaction was stimulated by vasopressin greater than isoproterenol greater than calcitonin. PTH had no effect. In cortical thick ascending loop of Henle cells, the stimulation was by calcitonin greater than vasopressin = PTH. Isoproterenol had no effect. In medullary ascending loop epithelia, stimulation was by vasopressin = calcitonin. Neither isoproterenol nor PTH had an effect. These observations indicate that adenylate cyclase is histochemically demonstrable in cultivated cells from rabbit distal nephron segments and that the enzyme activation by hormones is differential according to the epithelium of origin.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology; D002116:Calcitonin / Q000494:pharmacology; D046508:Culture Techniques; D004848:Epithelium / Q000201:enzymology; D006651:Histocytochemistry; D006728:Hormones / Q000494:pharmacology*; D007545:Isoproterenol / Q000494:pharmacology; D009399:Nephrons / Q000201:enzymology*; D010281:Parathyroid Hormone / Q000494:pharmacology; D011817:Rabbits
D006728:Hormones; D010281:Parathyroid Hormone; D001127:Arginine Vasopressin; D002116:Calcitonin; D000262:Adenylyl Cyclases; D007545:Isoproterenol