In situ molecular weight determination of rat brain [3H]phenytoin binding sites.
Using irradiation inactivation analysis of specific [3H]phenytoin binding to rat brain we have demonstrated that there are two different binding sites involved, with molecular weights of 73238 +/- 1535 (higher affinity site) and 108121 +/- 6935 (lower affinity site).
D000818:Animals; D001921:Brain / Q000378:metabolism*; D005612:Freeze Drying; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008970:Molecular Weight; D010672:Phenytoin / Q000378:metabolism*; D051381:Rats; D011955:Receptors, Drug / Q000032:analysis* / Q000528:radiation effects
D011955:Receptors, Drug; C029450:phenytoin receptor; D010672:Phenytoin