A readily obtainable analog of propranolol suitable for use as internal standard in chromatographic assays for propranolol.
There appears to be a need for a readily and conveniently available close chemical analog of propranolol suitable for use as internal standard in a variety of chromatographic assays of the drug. With this purpose in mind, we developed a simple procedure for the preparation of N-cyclopentyldesisopropylpropranolol. The commercially available and inexpensive starting materials are mixed in a flask and after 48 hr at room temperature the precipitated product is filtered off. Mass spectrometry, melting point determinations, and elemental analysis were used to characterize the product. N-cyclopentyldesisopropylpropranolol was used as internal standard in a liquid-chromatographic assay of ten replicate 50 ng/ml propranolol plasma samples, and gave a coefficient of variation of 3.4%.
D002853:Chromatography, Liquid; D006801:Humans; D011433:Propranolol / Q000031:analogs & derivatives* / Q000097:blood* / Q000138:chemical synthesis; D012015:Reference Standards
C046154:N-cyclopentyldeisopropylpropranolol; D011433:Propranolol