"New" autoantibody specificity in autoimmune hemolytic anemia defined with red cells treated with 2-aminoethylisothiouronium bromide and a dithiothreitol-papain solution.
Specificity of IgG autoantibodies that react with random red cell samples but not with cells treated with 2-aminoethylisothiouronium bromide (AET)-previously was related to the Kell blood group system. We now describe an IgG autoantibody, from a patient who died with autoimmune hemolytic anemia, that reacted with random red cell samples, Ko cells, and with cells treated with a dithiothreitol-papain solution, but did not react with AET-treated cells or with Rhnull cells. Serological reactivity of the autoantibody is unlike that of IgG autoantibodies that do not react only with Rhnull cells. It appears that the reactive antigen is not part of the Kell or Rh systems, but is probably modified by the membrane anomaly present in Rhnull red cells.
D000368:Aged; D000744:Anemia, Hemolytic, Autoimmune / Q000276:immunology*; D000918:Antibody Specificity; D001323:Autoantibodies / Q000276:immunology*; D004229:Dithiothreitol / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects; D005260:Female; D006801:Humans; D010206:Papain / Q000494:pharmacology; D000338:beta-Aminoethyl Isothiourea / Q000494:pharmacology
D001323:Autoantibodies; D000338:beta-Aminoethyl Isothiourea; D010206:Papain; D004229:Dithiothreitol