Acute effects of tumor-promoting phorbol esters on hepatic intermediary metabolism.
In hepatocytes isolated from meal-fed rats, phorbol 12-myristate 13-acetate as well as phorbol 12,13-didecanoate stimulated de novo fatty acid synthesis in a dose-dependent manner. Moreover, phorbol 12-myristate 13-acetate inhibited ketogenesis from exogenous oleate, but slightly enhanced oleate esterification. The stimulation of esterification was more pronounced with endogenously synthesized fatty acids. In hepatocytes from 24h-starved rats a moderate stimulation of gluconeogenesis and ureogenesis was observed with glutamine as substrate. It is concluded that tumor-promoting phorbol esters mimic the short-term effects of insulin on hepatic fatty acid metabolism.
D000818:Animals; D005227:Fatty Acids / Q000096:biosynthesis; D005502:Food; D005943:Gluconeogenesis / Q000187:drug effects; D005973:Glutamine / Q000378:metabolism; D007657:Ketone Bodies / Q000096:biosynthesis; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010703:Phorbol Esters / Q000494:pharmacology*; D010704:Phorbols / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013217:Starvation / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D005227:Fatty Acids; D007657:Ketone Bodies; D010703:Phorbol Esters; D010704:Phorbols; D005973:Glutamine; C008876:phorbol-12,13-didecanoate; D013755:Tetradecanoylphorbol Acetate; C033085:phorbol