Tumor promoter phorbol-12-myristate-13-acetate induces poly(ADP)-ribosylation in fibroblasts.
The tumor promoter phorbol-12-myristate-13-acetate (PMA) causes an increase in pol(ADP)-ribosylation in mouse and human fibroblasts via the intermediate formation of active oxygen. In contrast to poly(ADP)-ribosylation induced by the methylating agent N-methyl-N'-nitro-N-nitrosoguanidine, de novo RNA and protein synthesis are required and the accumulation of the polymer occurs in the absence of detectable DNA strand breakage. Our results suggest a mechanism for PMA-induced modulation of chromatin structure and gene expression.
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002478:Cells, Cultured; D004247:DNA; D005347:Fibroblasts / Q000378:metabolism; D008769:Methylnitronitrosoguanidine; D051379:Mice; D008809:Mice, Inbred C3H; D009702:Nucleoside Diphosphate Sugars / Q000096:biosynthesis*; D010100:Oxygen / Q000378:metabolism; D010704:Phorbols / Q000633:toxicity*; D011064:Poly Adenosine Diphosphate Ribose / Q000096:biosynthesis*; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity*
D000975:Antioxidants; D009702:Nucleoside Diphosphate Sugars; D010704:Phorbols; D008769:Methylnitronitrosoguanidine; D011064:Poly Adenosine Diphosphate Ribose; D004247:DNA; D013755:Tetradecanoylphorbol Acetate; D010100:Oxygen