title: 5'-Iodothyronine deiodinase of rat liver: activity in microsomes prepared by various methods, solubilization by detergents and partial purification.
abstract: Iodothyronine deiodinase activities of rat liver microsomes prepared by (1) differential centrifugation, (2) column chromatography, (3) precipitation with Ca2+, (4) precipitation at low pH, or combinations of these were compared. Method 2 or 2 followed by 4 provided microsomes with specific activities 4.6- and 7.4-times higher than method 1, respectively. Both Triton X-100 at 0.1% (w/v) and 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate (Chaps) at 4-6 mM efficiently solubilized deiodinase and were not inhibitory at low concentrations. The Chaps-soluble enzyme could be moderately purified by fractionation with ammonium sulfate but more effectively with poly(ethylene glycol).
mesh_terms: D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D011232:Chemical Precipitation; D003902:Detergents; D007453:Iodide Peroxidase / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D010544:Peroxidases / Q000378:metabolism*; D051381:Rats; D012995:Solubility; D014285:Triiodothyronine, Reverse / Q000378:metabolism*
chemical_list: D003902:Detergents; D014285:Triiodothyronine, Reverse; D010544:Peroxidases; D007453:Iodide Peroxidase