3-Aminobenzamide sensitizes cultured Chinese hamster cells with bromodeoxyuridine-substituted DNA to genetic damage induced by long-wave UV.
Chinese hamster cells, containing BrdUrd-substituted DNA, were irradiated in the presence of 3-aminobenzamide with short-wave UV, long-wave UV or X-rays and analyzed for induced SCEs or chromosomal aberrations. The data presented in this paper show that when BrdUrd-substituted cells are irradiated with lw-UV in the presence of 3-aminobenzamide, genetic damage is increased. Biochemical analysis shows that the molecular weight of BrdUrd-substituted DNA is reduced by this treatment. The sensitization is due to the combined action of lw-UV, incorporated BrdUrd and 3-aminobenzamide, without any involvement of inhibition of poly(ADP-ribose) synthetase. No potentiation occurs when cells are irradiated with X-rays and genetic damage is decreased when cells are irradiated with UV light of 254 nm in the presence of 3AB. This decrease coincides with a reduction in the amount of induced pyrimidine dimers, detected as T4 endonuclease-sensitive sites in DNA.
D000818:Animals; D001549:Benzamides / Q000494:pharmacology*; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000528:radiation effects*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure; D006801:Humans; D007231:Infant, Newborn; D009154:Mutation; D010053:Ovary; D010777:Photochemistry; D011740:Pyrimidine Dimers / Q000378:metabolism; D011836:Radiation Tolerance / Q000187:drug effects; D014466:Ultraviolet Rays; D014965:X-Rays
D001549:Benzamides; D011740:Pyrimidine Dimers; C025160:3-aminobenzamide; D004247:DNA; D001973:Bromodeoxyuridine