Protoplast water content of bacterial spores determined by buoyant density sedimentation.
Protoplast wet densities (1.315 to 1.400 g/ml), determined by buoyant density sedimentation in Metrizamide gradients, were correlated inversely with the protoplast water contents (26.4 to 55.0 g of water/100 g of wet protoplast) of nine diverse types of pure lysozyme-sensitive dormant bacterial spores. The correlation equation provided a precise method for obtaining the protoplast water contents of other spore types with small impure samples and indicated that the average protoplast dry density was 1.460 g/ml.
D001407:Bacillus / Q000032:analysis*; D002499:Centrifugation, Density Gradient / Q000379:methods; D008793:Metrizamide; D009113:Muramidase; D011523:Protoplasts / Q000032:analysis*; D013045:Species Specificity; D013171:Spores, Bacterial / Q000032:analysis*; D014867:Water / Q000032:analysis*
D014867:Water; D009113:Muramidase; D008793:Metrizamide