Isolation of alpha 1-acid glycoprotein from human plasma using high-performance liquid chromatography.
A method for the rapid isolation of purified alpha 1-acid glycoprotein (AGP) from small volumes of human plasma using HPLC has been developed. The method involves preparation of the seromucoid fraction of plasma by sequential perchloric acid and phosphotungstic acid precipitations, followed by chromatography on an HPLC TSKG-3000 column. The yield was high (0.75 mg AGP/ml plasma) and the procedure takes less than 1 day. The method lends itself to easy automation and is particularly suitable for isotopic turnover studies requiring multiple plasma samples.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007122:Immunoelectrophoresis; D008832:Microchemistry; D008970:Molecular Weight; D009961:Orosomucoid / Q000032:analysis / Q000302:isolation & purification*; D012794:Sialic Acids / Q000032:analysis
D009961:Orosomucoid; D012794:Sialic Acids