title: Ascaris suum: protective immunity in pigs immunized with products from eggs and larvae.
abstract: Parasite products were collected at three distinct phases of development of Ascaris suum, and their immunogenicity was determined after injection into rabbits and pigs. Products were derived from (1) the hatching fluid of infective eggs; (2) the conditioned medium of 2nd-stage larvae that developed to 3rd stage in vitro in defined medium; and (3) the conditioned medium of 3rd-stage larvae that developed to 4th stage in vitro in defined medium. Protein profiles from these three preparations, separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, were less complex than that of extracts from homogenized A. suum larvae. Hyperimmune rabbit antiserum raised against either egg products, 2nd- to 3rd-stage larval excretory-secretory products, or 3rd- to 4th-stage larval excretory-secretory products showed strong homologous reactions after immunoelectrophoresis, but relatively weak cross-reactions with the other preparations. A combined enteral immunization of pigs with egg products and parenteral immunization with the 2nd- to 3rd-stage larval excretory-secretory products, and 3rd- to 4th-stage larval excretory-secretory products induced antibody to each preparation and significant protective immunity to a challenge exposure with 10,000 A. suum eggs. However, a marked pathological response to larvae migrating in the liver after challenge exposure was also induced.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000947:Antigens, Helminth / Q000276:immunology*; D001196:Ascariasis / Q000276:immunology / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary*; D001200:Ascaris / Q000254:growth & development / Q000276:immunology*; D005260:Female; D007114:Immunization / Q000662:veterinary*; D007814:Larva / Q000276:immunology; D008099:Liver / Q000473:pathology; D008297:Male; D010063:Ovum / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control
chemical_list: D000947:Antigens, Helminth