Generating sucrose gradients in three minutes by tilted tube rotation.
A technique which permits rapid preparation of sucrose gradients with highly reproducible profiles is described. Tubes are filled with equal volumes of light and heavy sucrose solutions, sealed, and rotated for 3 min tilted 80 degrees from vertical. Linear 5-20% and 5-30% gradients and nonlinear but useful 5-45% gradients are obtained.
D002499:Centrifugation, Density Gradient / Q000379:methods*; D012399:Rotation; D013395:Sucrose
D013395:Sucrose