Distribution and reproducibility of aryl hydrocarbon hydroxylase inducibility in a prospective population study of middle-aged male smokers and nonsmokers.
Aryl hydrocarbon hydroxylase (AHH) inducibility was determined in a lymphoblast test system in 2,000 consecutive middle-aged male smokers, 304 ex-smokers, and 218 never-smokers in the same birth-year cohorts. Intraindividual, intraobserver, and interobserver, as well as temporal, reproducibility was checked in a special double-blind quadruplet sample series from 20 other consecutive middle-aged men. The results showed a three-modal phenotype distribution of AHH inducibility with high (fold induction greater than or equal to 3.6), intermediate (2.6-3.6) and low (less than or equal to 2.5) levels ranging between 7.6% to 10.5%, 38.5% to 43.0%, and 46.5% to 53.9%, respectively, in all the smoking categories. The reproducibility of the measurements was excellent, with one-way variance in the order of 0.007 to 0.033, and the applied assay method can therefore be used in large-scale prospective population investigations. Such are required in order to establish a cause-effect association between high AHH inducibility and smoking-related malignancies of the respiratory tract and oral cavity, as has been suggested from earlier retrospective studies in more limited clinical materials of cancers and precanceroses of these varieties.
D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism*; D004311:Double-Blind Method; D004790:Enzyme Induction; D004812:Epidemiologic Methods; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology*; D008297:Male; D008748:Methylcholanthrene / Q000378:metabolism; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000209:etiology; D010641:Phenotype; D011446:Prospective Studies; D012142:Respiratory Tract Neoplasms / Q000097:blood / Q000209:etiology; D012907:Smoking
D008748:Methylcholanthrene; D001189:Aryl Hydrocarbon Hydroxylases