DNA analysis and sorting of rat testis cells using two-parameter flow cytometry.
By use of two-parameter flow cytometry of rat testis cell suspensions stained with mithramycin for DNA (the peak amplitude of the fluorescence signal versus total fluorescence intensity integrated over time), eight cell compartments could be distinguished without pre-enrichment of the samples. Cells in these compartments were identified by sorting and subsequent microscopic examination.
D000818:Animals; D002469:Cell Separation; D004247:DNA / Q000032:analysis*; D005347:Fibroblasts / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods; D007985:Leydig Cells / Q000032:analysis; D008297:Male; D051381:Rats; D012708:Sertoli Cells / Q000032:analysis; D013087:Spermatids / Q000032:analysis; D013090:Spermatocytes / Q000032:analysis; D013093:Spermatogonia / Q000032:analysis; D013737:Testis / Q000166:cytology*
D004247:DNA