Isolation and partial characterization of a novel basement membrane collagen.
A guanidine-HCl extraction of lens capsule basement membrane dissolves collagenous material. This material was fractionated on an Agarose A-5M column. Fractions 1, 2 and 3 were further purified and partially characterized immunochemically and by amino acid analysis. Fraction 3 has a molecular weight of 55,000 when compared with collagen type I standard. The CNBr peptide pattern and composition of fraction 3 are different from those of alpha 1 (IV) 95K and alpha 2 (IV) 95K chains. The results described suggest the presence of a new chain in lens capsule basement membrane.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001485:Basement Membrane / Q000032:analysis*; D002417:Cattle; D002850:Chromatography, Gel; D003094:Collagen / Q000276:immunology / Q000302:isolation & purification*; D005109:Extracellular Matrix / Q000032:analysis*; D007908:Lens, Crystalline / Q000032:analysis; D008970:Molecular Weight
D000596:Amino Acids; D003094:Collagen