title: Measurement of immunoreactive leukotriene C4 in blood of asthmatic children.
abstract: Peptide leukotriene (LT) such as LTC4, LTD4, LTE4 have been considered to be major mediators of immediate type hypersensitivity reaction such as asthma. We have developed a rapid and simple extraction method using a Sep-Pak C18 cartridge for the measurement of LTC4 by radioimmunoassay (i-LTC4). In this extraction method, 91% LTC4 was recovered in a final methanol fraction. The identity was confirmed by the recovery test and by the dilution method. The amount of i-LTC4 in plasma from asthmatic patients was determined by radioimmunoassay after the extraction. The order of the plasma level of i-LTC4 was; severe asthma greater than slight or moderate asthma greater than asthmatic patient without attack greater than healthy adult. The highest level of LTC4 was 0.27 +/- 0.11 pmol/ml in severe asthmatic plasma.
mesh_terms: D000328:Adult; D001249:Asthma / Q000097:blood*; D002648:Child; D006801:Humans; D011863:Radioimmunoassay; D013189:SRS-A / Q000097:blood* / Q000276:immunology
chemical_list: D013189:SRS-A