title: The effects of neonatal thyroid deficiency on acetylcholine synthesis and glucose oxidation in rat corpus striatum.
abstract: The effects of propylthiouracil (PTU)-induced thyroid deficiency on [14C]acetylcholine synthesis and 14CO2 production from [U-14C]glucose in vitro, by fine prisms of the corpus striatum were investigated in developing rats. Consistent with deficits in choline uptake and choline acetyltransferase activity (Kalaria et al.17), PTU-treatment from two days after birth significantly impaired (27-39%) [14C]acetylcholine synthesis in striatal tissue taken from 3- or 6-week-old animals. In the thyroid-deficient (Tx) animals, 14CO2 production from [14C]glucose was unchanged in incubations in the presence of 5 mM K+ but was significantly reduced (33%) in medium with 31 mM K+ concentration. The addition of 10 mM DL-3-hydroxybutyrate in incubations with 5 mM K+ persistently inhibited 14CO2 production by striatal samples from the Tx rats. The fraction acetylated of [3H]choline accumulated by striatal prisms was unaffected by the PTU-induced thyroid deficiency. These findings suggest the development of fewer cholinergic nerve terminals in striatum during neonatal thyroid deficiency. Cholinergic nerve terminals that develop seem unaffected in their capacity for K+-stimulation and in their ability to acetylate transported [3H]choline.
mesh_terms: D020155:3-Hydroxybutyric Acid; D000109:Acetylcholine / Q000096:biosynthesis*; D000818:Animals; D000831:Animals, Newborn; D002794:Choline / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000494:pharmacology; D007037:Hypothyroidism / Q000139:chemically induced / Q000378:metabolism*; D066298:In Vitro Techniques; D010084:Oxidation-Reduction; D011188:Potassium / Q000494:pharmacology; D011441:Propylthiouracil; D051381:Rats
chemical_list: D006885:Hydroxybutyrates; D011441:Propylthiouracil; D005947:Glucose; D002794:Choline; D000109:Acetylcholine; D011188:Potassium; D020155:3-Hydroxybutyric Acid