Immunodetection with streptavidin-acid phosphatase complex on Western blots.
A technique for the detection of nanogram amounts of protein blotted onto nitrocellulose membranes has been developed using nonradioactive probes. Protein transferred to nitrocellulose membranes is detected by a specific antibody followed by incubation with biotinylated anti-antibody. After addition of streptavidin-acid phosphatase complex, incubation with fast violet B salt produces sharp magenta bands. This method allows detection of bands containing less than 20 ng of protein. The procedure does not use radioactive or carcinogenic materials.
D000135:Acid Phosphatase; D000818:Animals; D001360:Avidin; D001710:Biotin; D003101:Collodion; D003116:Color; D006735:Horseradish Peroxidase; D006801:Humans; D007118:Immunoassay; D010047:Ovalbumin / Q000031:analogs & derivatives*; D011506:Proteins / Q000032:analysis*; D011817:Rabbits
D011506:Proteins; D001360:Avidin; D001710:Biotin; D003101:Collodion; D010047:Ovalbumin; D006735:Horseradish Peroxidase; D000135:Acid Phosphatase