Fluorouracil (5-FU) and cytarabine (ara-C) inhibition of corneal epithelial cell and conjunctival fibroblast proliferation.
We evaluated the antiproliferative properties of fluorouracil (5-FU) and cytarabine (ara-C) in a tissue culture model of rabbit corneal epithelial cells and conjunctival fibroblasts. Cells were treated with fluorouracil or cytarabine for five days. Corneal epithelial cell proliferation was inhibited to 50% of control proliferation by 0.6 mg/L of fluorouracil or 30 micrograms/L of cytarabine. Proliferation of conjunctival fibroblasts was inhibited to 50% of control by 0.5 mg/L of fluorouracil or 50 micrograms/L of cytarabine. The antiproliferative effect of 10 mg/L of fluorouracil was not blocked by thymidine. The inhibition of proliferation produced by 100 or 1,000 micrograms/L of cytarabine was blocked by 2'-deoxycytidine at concentrations of 10(-5) M and 10(-4) M, respectively. The antiproliferative effects of fluorouracil and cytarabine appear similar for both cell types. Blockage of cytarabine-related ocular toxic effects may be possible with 2'-deoxycytidine.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003228:Conjunctiva / Q000166:cytology* / Q000187:drug effects; D003315:Cornea / Q000166:cytology* / Q000187:drug effects; D046508:Culture Techniques; D003561:Cytarabine / Q000494:pharmacology*; D004847:Epithelial Cells; D005347:Fibroblasts / Q000187:drug effects; D005472:Fluorouracil / Q000494:pharmacology*; D011817:Rabbits; D013936:Thymidine / Q000494:pharmacology
D003561:Cytarabine; D005472:Fluorouracil; D013936:Thymidine