Endogenous copper is cytotoxic to a lymphoma in primary culture which requires thiols for growth.
With the use of bathocuproine sulfonate, a copper-specific chelator as an indicator, we have demonstrated that copper ions, present as a natural medium constituent are toxic to the growth of a lymphoma in primary culture and are principally responsible for the growth requirement of mercaptoethanol and other thiols. By chelating trace copper normally present in the medium, bathocuproine sulfonate retarded the oxidation of cysteine to poorly utilized cystine, thus permitting its direct utilization by the cells for growth.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002614:Chelating Agents / Q000494:pharmacology; D003300:Copper / Q000633:toxicity*; D003545:Cysteine / Q000378:metabolism; D007939:Leukemia L1210 / Q000473:pathology*; D051379:Mice; D010084:Oxidation-Reduction; D010618:Phenanthrolines / Q000494:pharmacology*; D013438:Sulfhydryl Compounds / Q000502:physiology
D002614:Chelating Agents; D010618:Phenanthrolines; D013438:Sulfhydryl Compounds; C028559:bathocuproine sulfonate; D003300:Copper; D003545:Cysteine