Evidence for alpha-ketoglutarate dehydrogenase monomer activity with the aid of enzyme immobilization.
Immobilization of pigeon breast muscle alpha-ketoglutarate dehydrogenase on CNBr-activated Sepharose 4B was carried out. Conditions for dissociation of the dimeric enzyme bound to the carrier via a single subunit were determined. Immobilized enzyme monomers with a specific activity higher than that of the dimer were obtained. The immobilized subunits are capable of reassociating with the soluble ones; this is accompanied by the restoration of the initial amount of the matrix-bound protein and the reconstitution of the activity typical of the immobilized enzyme original preparations.
D000818:Animals; D010856:Columbidae; D004587:Electrophoresis, Agar Gel; D004800:Enzymes, Immobilized; D066298:In Vitro Techniques; D007655:Ketoglutarate Dehydrogenase Complex / Q000032:analysis / Q000037:antagonists & inhibitors*; D007658:Ketone Oxidoreductases / Q000037:antagonists & inhibitors*; D009132:Muscles / Q000201:enzymology*
D004800:Enzymes, Immobilized; D007658:Ketone Oxidoreductases; D007655:Ketoglutarate Dehydrogenase Complex