Adenylate cyclase of Dictyostelium discoideum: solubilization and Mn2+-dependency.
Adenylate cyclase from Dictyostelium discoideum was solubilized under alkaline conditions using the zwitterionic detergent CHAPS. In contrast to the membrane bound adenylate cyclase, the solubilized enzyme can use only Mn2+, but is inactive in the presence of Mg2+.
D000262:Adenylyl Cyclases / Q000302:isolation & purification / Q000378:metabolism*; D004023:Dictyostelium / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D012995:Solubility
D008345:Manganese; D000262:Adenylyl Cyclases; D008274:Magnesium