HPLC-studies on nonmercapt-mercapt conversion of human serum albumin.
Human mercaptalbumin (HMA) and nonmercaptalbumin (HNA) could be separated by high-performance liquid chromatography (HPLC) at neutral pH. Using HPLC, the present authors found the nonmercapt-mercapt conversion (HNA----HMA) during hemodialysis and the mercapt-nonmercapt conversion (HMA----HNA) after hemodialysis in chronic renal failure, indicating HMA as the covalent carrier protein for sulfur-containing amino acids.
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood; D006435:Renal Dialysis; D012709:Serum Albumin / Q000032:analysis*
D012709:Serum Albumin; C001702:mercaptoalbumin