Immunoprotective capability of somatic hybrid cells in comparison with parental tumor cells maintained in vitro.
The immunogenicity of X-irradiated hybrid cells derived from fusion of ASL-1 leukemia (A origin) and LM (TK-) fibroblasts (C3H origin) was compared to X-irradiated parental ASL-1 leukemia cells maintained in vivo (V-ASL-1) and to X-irradiated ASL-1 leukemia cells maintained in vitro (C-ASL-1). Immunization with hybrid cells induced transplantation resistance against tumor rechallenge with V-ASL-1 more effectively than did immunization with V-ASL-1 tumor cells. Immunization with X-irradiated C-ASL-1 cells produced the same, or slightly stronger level of transplantation resistance than that with X-irradiated hybrid cells. These findings were observed both in A/J and in (C3H/HeJ X A/J)F1 mice. These results raise a question about whether the apparent increased immunogenicity of hybrid cells is due to a result of cell fusion or a result of their growth in vitro.
D000818:Animals; D002478:Cells, Cultured / Q000528:radiation effects; D006822:Hybrid Cells / Q000276:immunology* / Q000528:radiation effects; D007114:Immunization; D007942:Leukemia, Experimental / Q000276:immunology*; D051379:Mice; D008805:Mice, Inbred A; D008809:Mice, Inbred C3H; D009368:Neoplasm Transplantation; D014181:Transplantation Immunology