Glycerol phosphate dehydrogenase activity of oligodendrocytes isolated from adult pig brain: its inducibility by hydrocortisone.
Developing oligodendrocytes cultured in vitro express glycerol phosphate dehydrogenase (GPDH; EC 1.1.1.8) and are known to respond to glucocorticoid treatment by increased activity of GPDH. We present evidence that GPDH is enriched in white matter and oligodendrocytes of adult pig brain. Bulk-isolated oligodendrocytes maintained in culture for several weeks exhibit an almost constant level of GPDH activity. Furthermore, a 4-day stimulation with hydrocortisone induces GPDH specific activity of long-term cultured oligodendrocytes from adult pig brain.
D000818:Animals; D001921:Brain / Q000166:cytology* / Q000201:enzymology; D002478:Cells, Cultured; D004790:Enzyme Induction; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+); D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism*; D006854:Hydrocortisone / Q000494:pharmacology*; D009457:Neuroglia / Q000201:enzymology*; D009836:Oligodendroglia / Q000187:drug effects / Q000201:enzymology*; D013552:Swine; D013997:Time Factors
D005993:Glycerolphosphate Dehydrogenase; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+); D006854:Hydrocortisone