Internalization of cycloheptaamylose-dansyl chloride complex during labelling of surface membrane in living Paramecium aurelia cells.
Internalization of cycloheptaamylose-dansyl chloride complex during surface labelling of living long-term starved Paramecium aurelia cells has been observed. This process may be inhibited by pretreatment of the ciliates with dichloroisoproterenol. Uptake of cycloheptaamylose-dansyl chloride may be visualized only after UV preirradiation: the appearance of orange-fluorescing vacuoles of diameter 2.3-4.5 micron may then be observed. Microspectrographic analysis performed on the cells and dansyl derivatives indicates that this fluorescence is produced by a photochemical reaction of dansyl chloride - released from CDC complex inside the digestive vacuoles-under the influence of UV irradiation.
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D003505:Cyclodextrins / Q000378:metabolism* / Q000528:radiation effects; D003619:Dansyl Compounds / Q000378:metabolism* / Q000528:radiation effects; D003912:Dextrins / Q000378:metabolism*; D004705:Endocytosis / Q000187:drug effects*; D005456:Fluorescent Dyes; D006813:Humidity; D007840:Latex / Q000378:metabolism; D010247:Paramecium / Q000378:metabolism*; D013053:Spectrophotometry; D013213:Starch / Q000378:metabolism*; D014617:Vacuoles / Q000032:analysis; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D003619:Dansyl Compounds; D003912:Dextrins; D005456:Fluorescent Dyes; D007840:Latex; D047392:beta-Cyclodextrins; C033483:cycloheptaamylose-dansyl chloride complex; D013213:Starch