Inhibition of association vs. dissociation of high-avidity DNA/anti-DNA complexes:possible involvement of secondary hydrogen bonds.
Recent results on the conditions of ionic strength needed to prevent the association of dsDNA with high avidity human anti-dsDNA, were compared with the insufficiency of even the highest practicable ionic strengths to effect the dissociation of such antigen-antibody complexes, once formed (1). Further analysis of these results make us conclude that such high avidity dsDNA-anti-dsDNA complexes, which in the initial stages of their formation are mainly of the Coulombic variety, subsequently evolve, at least in part, into hydrogen bonds.
D000906:Antibodies / Q000378:metabolism*; D000915:Antibody Affinity; D000936:Antigen-Antibody Complex; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004247:DNA / Q000276:immunology*; D006801:Humans; D006860:Hydrogen Bonding; D009994:Osmolar Concentration; D012965:Sodium Chloride / Q000494:pharmacology
D000906:Antibodies; D000936:Antigen-Antibody Complex; D012965:Sodium Chloride; D004247:DNA