Lack of effect of added liposomes on veratridine activation of 22Na+ uptake in rat brain synaptosomes.
Partially purified egg yolk phospholipids in the form of both small and large unilamellar liposomes at lipid concentrations of 30 mg/ml and higher inhibited veratridine activation of sodium channel specific 22Na+ uptake by rat brain synaptosomes. Chromatographically purified egg yolk phosphatidyl choline liposomes did not show this inhibition even at lipid concentrations up to 125 mg/ml. This demonstrates that the inhibition of veratridine-activated 22Na+ uptake was due to an impurity in the lipid rather than an ability of the liposomes to absorb and immobilize the lipophilic veratridine molecule.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D008081:Liposomes / Q000494:pharmacology*; D051381:Rats; D012964:Sodium / Q000378:metabolism*; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*; D014701:Veratridine / Q000494:pharmacology*; D014702:Veratrine / Q000031:analogs & derivatives*
D008081:Liposomes; D014701:Veratridine; D012964:Sodium; D014702:Veratrine