[Tryptophan operon of methylotrophic facultative Pseudomonas sp. M bacteria. I. Isolation and characterization of auxotrophic Trp-mutants].
Eighteen auxotropic trp- mutants of the facultative methylotrophic bacteria Pseudomonas sp. M. induced by nitrosoguanidine were characterized. Trp- mutants were tested for a number of biochemical properties: the capacity to grow on tryptophan intermediates, their accumulation in growth medium and activities of key enzymes. The trpE, trpD, trpC, trpF, trpB and trpA mutants were identified. The trpDC121 mutant with a one-point mutation has been obtained. This mutation caused inactivation of two enzymes--anthranilate-5-phosphoribosyl transferase and indole-3-glycerophosphate synthase. Unusual trpA and trpB auxotrophs with TrpAB- phenotype were described. It may be concluded that this type of mutations cause loss of catalytic activity of a subunit of tryptophan synthase as well as its structural modification. As a result, no active tryptophan synthase complex is formed and hence, the activity of the opposite intact subunit is inhibited.
D008697:Methane / Q000378:metabolism*; D009154:Mutation; D009604:Nitrosoguanidines / Q000494:pharmacology; D009876:Operon / Q000187:drug effects; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D014364:Tryptophan / Q000096:biosynthesis / Q000235:genetics*
D009604:Nitrosoguanidines; D014364:Tryptophan; D008697:Methane
Triptofanovyĭ operon fakul'tativnykh metilotrofnykh bakteriĭ Pseudomonas sp. M. Soobshchenie I. Vydelenie i kharakteristika auksotrofnykh Trp-mutantov.