Platelet cytoskeleton--membrane interactions.
The order of attachment of the purified platelet cytoskeletal proteins, F-actin, alpha-actinin and Actin Binding Protein (ABP) to the isolated platelet membrane has been investigated. Of the three proteins, only F-actin would directly interact with a membrane preparation that had been extensively washed to remove associated cytoskeletal proteins. alpha-Actinin would only add to the membrane to which F-actin had been re-attached and ABP only if both F-actin and alpha-actinin were present on the membrane. These studies provide some insight into the nature of the attachment of the platelet cytoskeleton to the cytoplasmic side of the membrane.
D000185:Actinin / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D001792:Blood Platelets / Q000648:ultrastructure*; D002352:Carrier Proteins / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D018260:Gelsolin; D006801:Humans; D008840:Microfilament Proteins
D000199:Actins; D002352:Carrier Proteins; D018260:Gelsolin; D008840:Microfilament Proteins; C016953:brevin; D000185:Actinin