Formation and metabolism of pungent principle of Capsicum fruits. XV. Microdetermination of capsaicin by high-performance liquid chromatography with electrochemical detection.
A new, highly sensitive method for quantitative separation and determination of capsaicin and dihydrocapsaicin by high-performance liquid chromatography with electrochemical detection is elaborated. The method employs a Cosmosil 5Ph column eluted with 100 mM potassium dihydrogenphosphate containing 45% acetonitrile (pH 5.0) at a flow-rate of 1.0 ml/min, and an electrochemical detector (at a potential of +750 mV versus the Ag/AgCl electrode). The detection limits for both capsaicin and dihydrocapsaicin were 12 pg (39 fmol) at a signal-to-noise ratio of 3:1. By applying this method, the biological half-life of capsaicin in the rat was investigated.
D000818:Animals; D002211:Capsaicin / Q000031:analogs & derivatives / Q000032:analysis* / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D005638:Fruit / Q000032:analysis*; D006207:Half-Life; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D002211:Capsaicin; C012906:dihydrocapsaicin