Reisolation of Ruminobacter parvum.
A cellulolytic gram-negative ovoid motile rod (strain GS III) was isolated from an in vitro incubation of ground barley straw in rumen fluid. The anaerobic, non-sporulating, mesophilic organism strongly answered the original description of Ruminobacter parvum (Kaars Sijpesteijn, 1948). The strain GS III fermented pyruvate, D-arabinose, D-xylose, cellobiose, sucrose, maltose, cellulose, dextrin, xylan and pectin. Products from cellobiose were D-lactate, ethanol, acetate, hydrogen and carbon dioxide. A heat-resistant factor in yeast extract that was not a B-vitamin, a metal or one of the volatile fatty acids was required for growth. The mol % G + C was 51.9. The organism was lost after prolonged subculture.
D000818:Animals; D001421:Bacteria, Anaerobic / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D002417:Cattle; D002482:Cellulose / Q000378:metabolism*; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D000431:Ethanol / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005232:Fatty Acids, Volatile / Q000378:metabolism; D005285:Fermentation; D006090:Gram-Negative Bacteria / Q000032:analysis / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D001467:Hordeum; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000494:pharmacology; D012417:Rumen / Q000382:microbiology*; D013696:Temperature
D003470:Culture Media; D004269:DNA, Bacterial; D005227:Fatty Acids; D005232:Fatty Acids, Volatile; D000431:Ethanol; D002482:Cellulose; D010100:Oxygen