Bioluminescent assay as a potential method of rapid susceptibility testing.
A method of rapid susceptibility testing by bioluminescent assay was developed. Correlation between the 50% inhibition dose of antimicrobics for bacterial adenosine triphosphate measured by bioluminescent assay and the minimum inhibitory concentration obtained by the broth dilution method was satisfactory. In the bioluminescent assay the incubation time required was only 90 min.
D000255:Adenosine Triphosphate / Q000032:analysis; D000667:Ampicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D002512:Cephalothin / Q000494:pharmacology; D005839:Gentamicins / Q000494:pharmacology; D008163:Luminescent Measurements; D008826:Microbial Sensitivity Tests; D008911:Minocycline / Q000494:pharmacology; D013997:Time Factors
D000900:Anti-Bacterial Agents; D005839:Gentamicins; D000667:Ampicillin; D000255:Adenosine Triphosphate; D008911:Minocycline; D002512:Cephalothin