title: [HPLC determination of troxerutin in plasma and urine following oral administration in man].
abstract: A high-performance liquid chromatographic method (HPLC) is described for the analysis of 3',4',7-tri-O-(beta-hydroxy-ethyl)-rutoside (troxerutin) in human plasma and urine. After separation of interfering substances on XAD-2 trihydroxyethylrutoside is converted to tetrahydroxyethylrutoside by 2-chlorethanol in alkaline medium. After HPLC-separation tetrahydroxyethylrutoside is quantified by fluorescence detection. The pharmacokinetics of troxerutin were measured in plasma after oral administration to man. The relative bioavailability of the drug from Venelbin was 97.8 +/- 37.1% compared to an aqueous standard solution.
mesh_terms: D000925:Anticoagulants / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006896:Hydroxyethylrutoside / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000652:urine; D012431:Rutin / Q000031:analogs & derivatives*
chemical_list: D000925:Anticoagulants; D006896:Hydroxyethylrutoside; D012431:Rutin; C005865:troxerutin
vernacular_title: HPLC-Bestimmung von Troxerutin im Plasma und Harn nach oraler Gabe am Menschen.