Comparison of controlled pore glass and Kieselguhr-polydimethylacrylamide composite as supports for solid-phase synthesis of 23-residue oligodeoxyribonucleotides in milligram amounts.
Two 23-residue oligodeoxyribonucleotides, corresponding to both strands of a DNA duplex at the OR3 site of bacteriophage lambda, have been synthesized in good yields and in milligram quantities by a solid-phase phosphotriester method using two different supports, Kieselguhr-polydimethylacrylamide composite and controlled pore glass. Rapid purification was possible using high-performance liquid chromatography on radial compression ion-exchange columns. The results and utility of the two supports are compared.
D000180:Acrylic Resins; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D007692:Diatomaceous Earth; D004591:Electrophoresis, Polyacrylamide Gel; D005898:Glass; D008832:Microchemistry; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000302:isolation & purification; D009841:Oligonucleotides / Q000138:chemical synthesis*
D000180:Acrylic Resins; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; C045790:kieselguhr-polydimethylacrylamide composite; D007692:Diatomaceous Earth