[Changes in the intensity of the fluorescence of potential-sensitive fluorescent probes in the active transport of Ca2+ in the fragmented sarcoplasmic reticulum].
The dependence of fluorescence intensity changes of potential-sensitive fluorescent probes 3,3'-dipropyl-2,2'-thyodicarbocianine and 1-anilino-8-naphtalenesulphonae on the ATP concentration during Ca2+ transport in fragmented SR of the rabbit skeletal muscle has been studied. An increase in the accumulation of Ca2+ in the SR vesicles caused by ATP is accompanied by an increase in the fluorescence intensity of the potential-sensitive probes. These fluorescence changes are related neither to ATP or Ca2+ effect but are coupled with cation accumulation inside the vesicles since they are not observed in the presence of either EGTA or triton X-100 or in the absence of Mg2+. The results obtained prove the membrane potential generation in SR in the course of ATP-dependent Ca2+ transport.
D000818:Animals; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism*; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D008564:Membrane Potentials; D009132:Muscles / Q000378:metabolism*; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*
D005456:Fluorescent Dyes; D002118:Calcium
Izmenenie intensivnosti fluorestsentsii potentsialchuvstvitel'nykh fluorestsentnykh zondov pri aktivnom transporte Ca2+ v fragmentirovannom sarkoplazmaticheskom retikulume.