Inhibition of mitogen-induced blastogenesis in human lymphocytes by T-2 toxin and its metabolites.
Concentrations of T-2, HT-2, 3'-OH T-2, 3'-OH HT-2, T-2 triol, and T-2 tetraol toxins which inhibited [3H]thymidine uptake in mitogen-stimulated human peripheral lymphocytes by 50% were 1.5, 3.5, 4.0, 50, 150, and 150 ng/ml, respectively. The results suggested that the initial hydrolysis of T-2 toxin and the hydroxylation of T-2 toxin to 3'-OH T-2 toxin did not significantly decrease the immunotoxicity of the parent molecule, whereas further hydrolysis to T-2 triol and T-2 tetraol toxins or hydroxylation to 3'-OH HT-2 toxin decreased in vitro toxicity for human lymphocytes.
D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008934:Mitogens / Q000494:pharmacology; D012717:Sesquiterpenes / Q000494:pharmacology*; D013605:T-2 Toxin / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*
D008934:Mitogens; D012717:Sesquiterpenes; D013605:T-2 Toxin; C012351:HT-2 toxin