title: Anion-exchange chromatography to determine the concentration of chloride in sweat for diagnosis of cystic fibrosis.
abstract: For the diagnosis of cystic fibrosis, we use the "Macroduct Sweat Collection System" (Wescor) to sample sweat, and anion-exchange chromatography to determine its chloride content. The chromatographic system consists of a Whatman Partisil SAX 10-microns column, an isocratic phosphate-buffered mobile phase, and ultraviolet detection at 200 nm. The sweat chloride concentrations of 10 individuals with cystic fibrosis ranged from 101 to 131 mmol/L (mean 111, SD 9 mmol/L). For 11 controls the values ranged from 13 to 54 mmol/L (mean 28, SD 11 mmol/L). We conclude that the procedure is reliable for the diagnosis of cystic fibrosis.
mesh_terms: D000328:Adult; D002648:Child; D002675:Child, Preschool; D002712:Chlorides / Q000032:analysis*; D002852:Chromatography, Ion Exchange / Q000379:methods; D003550:Cystic Fibrosis / Q000175:diagnosis*; D005260:Female; D006801:Humans; D008297:Male; D009994:Osmolar Concentration; D013542:Sweat / Q000032:analysis*
chemical_list: D002712:Chlorides