Inhibition of initiation of simian virus 40 chromosome synthesis by dihydroxyanthraquinone.
The mode of action of dihydroxyanthraquinone, a new antitumor drug, on eucaryotic chromosome structure and function was investigated using simian virus 40 as a model system. Dihydroxyanthraquinone specifically inhibited initiation of viral replicons. Little or no viral DNA synthesis was recovered in cells after the removal of the drug. Elongation and termination of DNA already initiated could proceed continuously to completion in drug-treated cells. The drug appeared to be stably associated with viral chromosomes in cells. The irreversible inhibition of replicon initiation might contribute to its anti-proliferative and anti-neoplastic activity.
D000818:Animals; D000880:Anthraquinones / Q000494:pharmacology*; D002460:Cell Line; D002522:Chlorocebus aethiops; D002875:Chromosomes / Q000187:drug effects; D004261:DNA Replication / Q000187:drug effects*; D004278:DNA, Superhelical / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis; D007364:Intercalating Agents / Q000494:pharmacology; D008942:Mitoxantrone; D013329:Structure-Activity Relationship; D014779:Virus Replication / Q000187:drug effects*
D000880:Anthraquinones; D004278:DNA, Superhelical; D004279:DNA, Viral; D007364:Intercalating Agents; D008942:Mitoxantrone