Discovery of a gamma-carboxyglutamic acid-containing protein in human spermatozoa.
Here we describe the identification of a gamma-carboxyglutamic acid-containing protein in human spermatozoa. After thermal decarboxylation the protein is a good substrate for vitamin K-dependent carboxylase from various origins. A quick purification procedure for the decarboxylated protein is presented and in a preliminary characterization we have established its Mr (28 000-30 000) and its amino acid composition.
D015055:1-Carboxyglutamic Acid / Q000032:analysis*; D000596:Amino Acids / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D003653:Decarboxylation; D005971:Glutamates / Q000032:analysis*; D006801:Humans; D008297:Male; D011506:Proteins / Q000302:isolation & purification*; D013094:Spermatozoa / Q000032:analysis*
D000596:Amino Acids; D005971:Glutamates; D011506:Proteins; D015055:1-Carboxyglutamic Acid