Liquid chromatographic determination of the herbicide diuron and its metabolite 3,4-dichloroaniline in asparagus.
A liquid chromatographic method is presented for the determination of the phenylurea herbicide diuron and its major metabolite, 3,4-dichloroaniline in asparagus. The method involves a simple isolation step using dichloromethane extraction, followed by reversed phase chromatography on a C18 column, using a basic aqueous-organic eluent and UV detection at 254 nm. The limit of determination is 0.02 mg/kg for both analytes.
D000814:Aniline Compounds / Q000032:analysis*; D002853:Chromatography, Liquid; D004237:Diuron / Q000032:analysis*; D014675:Vegetables / Q000032:analysis*
D000814:Aniline Compounds; C014464:3,4-dichloroaniline; D004237:Diuron