In vitro synthesis of IgE in atopic and nonatopic individuals: the effects of pokeweed mitogen, Staphylococcus aureus-derived mitogens, and cycloheximide.
We studied the production of immunoglobulin (Ig)E in cultures containing mononuclear cells obtained from atopic and nonatopic subjects. The two groups did not differ in their baseline production of IgE. Pokeweed mitogen (PWM) and three different products of Staphylococcus aureus: purified protein A, heat-killed formalinized bacteria, and double-formalinized bacteria--induced a significant increase in the in vitro production of IgE. Mononuclear cells from both atopic and nonatopic individuals were stimulated to the same degree by all four mitogens. The increased production of IgE by PWM was totally abolished by cycloheximide; hence it was due to de novo protein synthesis.
D000293:Adolescent; D000328:Adult; D003513:Cycloheximide / Q000494:pharmacology*; D005260:Female; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000378:metabolism*; D007073:Immunoglobulin E / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008297:Male; D011043:Pokeweed Mitogens / Q000494:pharmacology*; D013211:Staphylococcus aureus
D011043:Pokeweed Mitogens; D007073:Immunoglobulin E; D003513:Cycloheximide