The characterization of [3H]sulpiride binding sites in pig striatal membranes.
Pig striatal membranes have [3H]sulpiride-binding sites similar to those identified in rat striatal membranes. The pharmacological profile indicates that this binding is to dopamine receptors. Agonist displacement of [3H]sulpiride binding in pig striatal membranes is subject to guanine nucleotide regulation. This effect is mimicked by heat treatment. N-ethyl maleamide (20 microM) and dithioerythritol (3 mM) decrease agonist affinity for the [3H]sulpiride-binding site in pig striatal membranes without significantly affecting maximal displacement.
D000818:Animals; D001665:Binding Sites; D003342:Corpus Striatum / Q000378:metabolism*; D005260:Female; D006150:Guanine Nucleotides / Q000494:pharmacology; D006358:Hot Temperature; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D051381:Rats; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D013469:Sulpiride / Q000378:metabolism*; D013552:Swine; D013764:Tetrahydronaphthalenes / Q000494:pharmacology; D014316:Tritium
D006150:Guanine Nucleotides; D013439:Sulfhydryl Reagents; D013764:Tetrahydronaphthalenes; D014316:Tritium; C011639:ADTN; D013469:Sulpiride