[A specific spectrophotometric method for determining plasma and/or serum hemoglobin. Use of the chromogen 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonate (ABTS)].
The authors describe a method for the determination of the plasma and/or serum haemoglobin, based on the peroxidase properties of the Haemoglobin-Haptoglobin complex, following the action of a solution of ABTS and hydrogen peroxide. The colour observed after a defined period of time is measured by spectrophotometry at 402 nm and is proportional to the haemoglobin concentration of the sample. A statistical study of the plasma haemoglobin concentration performed in male and female blood donors aged between 18 and 60 years is also presented.
D000293:Adolescent; D000328:Adult; D001560:Benzidines; D052160:Benzothiazoles; D001782:Blood Donors; D002863:Chromogenic Compounds; D005260:Female; D006242:Haptoglobins / Q000378:metabolism; D006454:Hemoglobins / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D010544:Peroxidases / Q000378:metabolism; D010949:Plasma / Q000032:analysis; D013053:Spectrophotometry / Q000379:methods; D013451:Sulfonic Acids
D001560:Benzidines; D052160:Benzothiazoles; D002863:Chromogenic Compounds; D006242:Haptoglobins; D006454:Hemoglobins; D013451:Sulfonic Acids; C035492:haptoglobin-hemoglobin complex; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D006861:Hydrogen Peroxide; D010544:Peroxidases
Méthode spectrophotométrique spécifique de détermination de l'hémoglobine plasmatique et/ou sérique. Utilisation du chromogène 2,2'-azino-di-3-(éthyl-benzothiazoline sulfonate-6) (ABTS).