Hydrophobic photolabelling of sarcoplasmic reticulum with [125I]TID.
[125I]TID, a small photoreactive lipophylic reagent, was used to label intrinsic proteins of rabbit and rat sarcoplasmic reticulum membranes. A 160,000 glycoprotein, the Ca2+-ATPase and polypeptides of mol. wt 53-55,000, 30,000, 20,000 and 6000 dalton were labelled suggesting that these proteins are integral membrane components.
D000818:Animals; D001389:Azirines; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D008565:Membrane Proteins / Q000032:analysis; D008970:Molecular Weight; D011817:Rabbits; D051381:Rats; D012519:Sarcoplasmic Reticulum / Q000032:analysis*
D001389:Azirines; D007457:Iodine Radioisotopes; D008565:Membrane Proteins; C033174:3-(trifluoromethyl)-3-(3-iodophenyl)diazirine