title: A study of dynamic development in radiolesion and cell death of eukaryotes after exposure to ionizing radiation--the visualization of the dynamic reaction by means of cytochemical method with lactate dehydrogenase (LDH) as a nuclear marker and radiation-induced nuclear anomalization by an ultramicrostructural manifestation: a dynamic explanation.
abstract: The present work deals with the radiation effect on adult rats suffering from acute, whole-body irradiation with cobalt-60 gamma rays of dosages 100R and 300R respectively. The morphological alteration of the lymphocytic nucleus and exhibition of cytochemical reaction of LDH in intra- or extra-nuclear region has been observed at the end of predetermined time intervals after irradiation. A close relationship between the percentage of damage of the lymphocytic nucleus and that of positivity of LDH reaction is seen from their variations with time parallel. From technical color microphotographs which exhibit cytochemical reactions, the existence of enzyme LDH in the nucleus so far obscure has been visually recognized. The events happening in enzymic reaction and the leakage of enzyme across the nuclear envelope reflect the dynamic nature. The ultrastructural alteration of nuclear anomalization confirms the dynamic development of inducing radiolesion. The experimental facts inspire us to attempt an explanation of cellular radiolesion and cell death owing to ionization radiation in light of the dynamic concept.
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology* / Q000648:ultrastructure; D002470:Cell Survival / Q000528:radiation effects*; D005057:Eukaryotic Cells / Q000528:radiation effects; D005720:Gamma Rays; D006651:Histocytochemistry; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008214:Lymphocytes / Q000528:radiation effects*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007770:L-Lactate Dehydrogenase