title: The sequence and topology of human complement component C9.
abstract: A partial nucleotide sequence of human complement component C9 cDNA representing 94% of the coding region of the mature protein is presented. The amino acid sequence predicted from the open reading frame of this cDNA concurs with the amino acid sequence at the amino-terminal end of three proteolytic fragments of purified C9 protein. No long stretches of hydrophobic residues are present, even in the carboxy-terminal half of the molecule which reacts with lipid-soluble photoaffinity probes. Monoclonal antibody epitopes have been mapped by comparing overlapping fragments of C9 molecule to which the antibodies bind on Western blots. Several of these epitopes map to small regions containing other surface features (e.g., proteolytic cleavage sites and N-linked oligosaccharide). The amino-terminal half of C9 is rich in cysteine residues and contains a region with a high level of homology to the LDL receptor cysteine-rich domains. A model for C9 topology based on these findings is proposed.
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D003186:Complement C9 / Q000235:genetics; D004274:DNA, Recombinant; D006801:Humans; D010446:Peptide Fragments / Q000276:immunology; D011487:Protein Conformation; D011973:Receptors, LDL / Q000235:genetics
chemical_list: D000911:Antibodies, Monoclonal; D003186:Complement C9; D004274:DNA, Recombinant; D010446:Peptide Fragments; D011973:Receptors, LDL