[Induction of microsomal cytochromes in the rat liver after intravenous administration of emulsions of perfluoro-organic compounds].
Intravenous injections of perfluoroorganic emulsions to rats in a dose of 3 ml/kg led to changes in the composition and activity of enzymes of the liver microsomal membrane monooxygenase system. At the peak of induction, i. e., on the 3rd post-injection day, the levels of microsomal cytochromes P-450 and b5 increased 2.8- and 1.9-fold, respectively, as compared to the control. Simultaneously, the rate of NADPH oxidation in the microsomes and the rate of hydroxylation of substrates I and II showed an increase. Conversely, the rate of NADPH-dependent peroxidation of microsomal lipids on the 2nd-4th post-injection days reached its minimal values. These injections stimulated the detoxicating function of rat liver as evidenced from the duration of the hexenal sleep of the animals. All the changes in the monooxygenase system parameters were temporary and reached the control level on the 10th-14th days after injection. It was demonstrated that the main component of the perfluoroorganic emulsions, perfluorodecalin, was responsible for the induction of the monooxygenase system enzymes.
D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004790:Enzyme Induction / Q000187:drug effects; D005466:Fluorocarbons / Q000008:administration & dosage / Q000494:pharmacology*; D006900:Hydroxylation; D007275:Injections, Intravenous; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains
D005466:Fluorocarbons; D008054:Lipid Peroxides; D001564:Benzo(a)pyrene; D009249:NADP; D003577:Cytochrome P-450 Enzyme System
Induktsiia mikrosomal'nykh tsitokhromov v pecheni krys posle vnutrivennogo vvedeniia zhivotnym émul'sii perftoroorganicheskikh soedineniĭ.