The effect of some experimental parameters on the inhibition of metabolic cooperation by phorbol myristate acetate.
The effect of cell density, PMA exposure time, concentration, pre-exposure and binding activity on the recovery of V 79 HGPRT- Chinese hamster cells in the metabolic cooperation assay was determined. A PMA exposure interval of only 1 min resulted in maximum recovery of HGPRT- cells. PMA began to inhibit metabolic cooperation at a dose as low as 0.1 ng/ml final media concentration. The recovery of HGPRT- cells varied according to cell density in the presence or absence of PMA, although the magnitude of this effect was much greater in untreated cells. Pre-exposure of cells to PMA increased the recovery of both post-PMA-treated and non-treated HGPRT- cells in a dose-dependent manner. [3H]PMA was rapidly bound to or taken up by V 79 cells. These results suggest that the inhibitory effect of PMA on metabolic cooperation in V 79 cells involves receptor binding.
D000818:Animals; D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D002450:Cell Communication / Q000187:drug effects*; D002452:Cell Count; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000378:metabolism; D007041:Hypoxanthine Phosphoribosyltransferase / Q000032:analysis; D010704:Phorbols / Q000494:pharmacology*; D011493:Protein Kinase C; D011955:Receptors, Drug; D011971:Receptors, Immunologic / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000378:metabolism / Q000494:pharmacology*; D013997:Time Factors; D014316:Tritium
D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D010704:Phorbols; D011955:Receptors, Drug; D011971:Receptors, Immunologic; C083141:phorbol ester binding protein; C027776:phorbol ester receptor; D014316:Tritium; D007041:Hypoxanthine Phosphoribosyltransferase; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate