Heat-induced inhibition of DNA synthesis in L5178Y-S cells is reversed by caffeine.
Heating L5178Y cells for 15 min at 43 degrees C caused a decrease in [3H]thymidine incorporation, which could be reversed by post-treatment with 0.75 mM caffeine in an L5178Y-S (radiation-sensitive, heat-resistant) but not in an L5178Y-R (radiation-resistant, heat-sensitive) strain. The reversal was accompanied by a sparing effect of the treatment: survival of L5178Y-S cells increased by a factor of 1.5. The effect of combined (heat + caffeine) treatment of L5178Y-R cells was cumulative.
D000818:Animals; D002110:Caffeine / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D006358:Hot Temperature; D008223:Lymphoma; D051379:Mice; D011836:Radiation Tolerance; D013936:Thymidine / Q000378:metabolism
D004273:DNA, Neoplasm; D002110:Caffeine; D013936:Thymidine