[DNA content and synthesis in the nuclei of rat cerebellar cells in organotypic cultures].
Using cytophotometric and autoradiographic methods, it has been shown for the first time that in condition of an organotypic culture the replicative synthesis of DNA is induced in the Purkinje neurons of the cerebellum of newborn rats completing their terminal differentiation. This synthesis is accompanied by polyploidization of the initially diploid population of these cells (4c, much more rarely 8c, and a single 16c cell appear) rather than by cell division. In constant, the granular cells mostly retain their diploid state and only a few of them synthesize DNA to H2c values. The glial cells divide actively. Hence, evidence is presented that neurons, at least those of cerebellum, retain their potential of replicative synthesis of DNA in the organotypic culture. The important point is that DNA synthesis in their nuclei proceeds simultaneously with processes of differentiation.
D000818:Animals; D000831:Animals, Newborn; D001345:Autoradiography; D002454:Cell Differentiation; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism*; D003592:Cytophotometry; D004247:DNA / Q000096:biosynthesis*; D004261:DNA Replication; D006651:Histocytochemistry; D009924:Organ Culture Techniques; D011689:Purkinje Cells / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D013997:Time Factors
D004247:DNA
Soderzhanie i sintez DNK v iadrakh kletok mozzhechka krysy v usloviiakh organotipicheskoĭ kul'tury.