title: Methods for study of mutations and mutagenesis in human lymphocytes.
abstract: Detailed methods are presented for measurement and study of in vivo mutations and in vitro mutagenesis in human lymphocytes. The methods described include preparation of conditioned medium containing interleukin-2, enumeration of mutant clones, in vitro mutagenesis, and expansion of mutant clones for further study.
mesh_terms: D002999:Clone Cells; D003470:Culture Media; D006801:Humans; D008214:Lymphocytes / Q000166:cytology*; D009152:Mutagenicity Tests / Q000379:methods*; D009154:Mutation; D010835:Phytohemagglutinins / Q000494:pharmacology
chemical_list: D003470:Culture Media; D010835:Phytohemagglutinins