Isolation of a mammalian sequence capable of conferring cell cycle regulation to a heterologous gene.
A hybrid gene containing the 5' sequence of a hamster histone H3 gene and the coding sequence of the bacterial neomycin-resistance gene (neo) was constructed. Upon transfection into the hamster fibroblast cell line K12, the hybrid gene exhibited cell cycle-dependent regulation, as evidenced by the maximal accumulation of the neo transcripts during synthesis of DNA in the cell cycle. In addition, cells arrested in the prereplicative phase, as a consequence of the K12 temperature-sensitive mutation, produced significantly less neo messenger RNA.
D000818:Animals; D002453:Cell Cycle; D006224:Cricetinae; D004261:DNA Replication; D004274:DNA, Recombinant; D004351:Drug Resistance; D005786:Gene Expression Regulation; D006657:Histones / Q000235:genetics*; D009355:Neomycin / Q000494:pharmacology; D011061:Poly A / Q000235:genetics; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D006657:Histones; D011061:Poly A; D009355:Neomycin