Different effects of nerve growth factor on cultured sympathetic and sensory neurons.
Long-term cultures of dissociated nodose ganglion (NG) and superior cervical ganglion (SCG) neurons from newborn rabbits were used to compare their response to nerve growth factor (7S NGF). SCG neurons required added NGF for their survival and a concentration of 1 microgram/ml was found to be optimal. NG neurons, on the other hand, survived well for a long term without addition of NGF, but its application (1 microgram/ml) was found to be effective in accelerating the growth of fibers (neurites) and neuronal somata. It is concluded that unlike SCG, NG neurons do not depend on exogenous NGF but may require an intrinsic trophic-like factor which may be contained in the serum of the medium, emanating from glial cells or by metabolic cooperation between neurons.
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D005728:Ganglia, Sympathetic; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009475:Neurons, Afferent / Q000187:drug effects*; D009620:Nodose Ganglion; D011817:Rabbits; D013564:Sympathetic Nervous System / Q000187:drug effects*
D009414:Nerve Growth Factors