Isolation of a photoreactivation-deficient mutant of Chlamydomonas.
A mutant deficient in photoreactivation has been isolated following mutagenesis of Chlamydomonas reinhardi with N-methyl-N'-nitro-N'-nitrosoguanidine. The mutant is deficient in the photorepair of pyrimidine dimers from nuclear DNA but appears to be normal in the rate of photorepair of dimers from chloroplast DNA. Cell-free extracts prepared from the photoreactivation-deficient mutant have about 17% of the DNA photolyase activity of wild-type cells. These results are consistent with the hypothesis that nuclear and chloroplast DNA photolyases are controlled by two separate genes.
D002696:Chlamydomonas / Q000235:genetics* / Q000302:isolation & purification; D004260:DNA Repair; D004271:DNA, Fungal / Q000378:metabolism; D008027:Light; D009154:Mutation; D010782:Photolysis
D004271:DNA, Fungal