An in vitro approach to the study of target organ toxicity of drugs and chemicals.
A major goal of our laboratory has been the development of primary culture systems that retain differentiated functions and responses characteristic of intact tissues in vivo. Specifically, we have developed cellular models of primary cultures of rat heart, liver, and kidney cells to explore the mechanisms by which drugs or chemicals may be toxic to key organs of the body and to develop new techniques by which xenobiotics may be evaluated or identified as potential toxicants to living systems. The purpose of this paper is to describe our rationale and approach to the study of target organ toxicology with in vitro cellular systems.
D000082:Acetaminophen / Q000378:metabolism / Q000633:toxicity; D000818:Animals; D000929:Antidepressive Agents, Tricyclic / Q000633:toxicity; D001711:Biotransformation; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002509:Cephaloridine / Q000633:toxicity; D006321:Heart / Q000187:drug effects*; D007545:Isoproterenol / Q000633:toxicity; D007672:Kidney Cortex / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008670:Metals / Q000633:toxicity; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000166:cytology; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid; D051381:Rats; D014116:Toxicology / Q000379:methods*; D014508:Urea / Q000378:metabolism
D000929:Antidepressive Agents, Tricyclic; D007773:Lactates; D008670:Metals; D011773:Pyruvates; D019344:Lactic Acid; D000082:Acetaminophen; D019289:Pyruvic Acid; D014508:Urea; D007545:Isoproterenol; D002509:Cephaloridine