Human lymphocyte glucocorticoid receptors reside mainly in the cytoplasm.
Karyoplasts prepared from human lymphocytes by treatment with cytochalasin B or lymphocytes permeabilized with digitonin contain only 25-40% of the total cellular glucocorticoid binding activity. The presented results thus support the original concept that unfilled steroid hormone receptors are mainly cytoplasmic.
D002463:Cell Membrane Permeability / Q000187:drug effects; D003571:Cytochalasin B / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism*; D004072:Digitonin / Q000494:pharmacology; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D014222:Triamcinolone Acetonide / Q000378:metabolism
D011965:Receptors, Glucocorticoid; D003571:Cytochalasin B; D014222:Triamcinolone Acetonide; D004072:Digitonin