title: Antileukotoxin antibody produced in the bovine lung after aerosol exposure to viable Pasteurella haemolytica.
abstract: Experiments were performed to determine the in vivo immunogenicity of Pasteurella haemolytica leukotoxin. Calves were exposed twice to aerosol mists of viable P haemolytica, using a treatment regimen previously shown to induce a resistant state. Pulmonary lavage fluids and serum samples from these calves were assayed for leukotoxin-neutralizing antibodies. Before aerosol exposure, neutralizing antibody titers were routinely found in serum samples, but were not detectable in pulmonary lavage concentrates before exposure. After aerosol exposure, titers of toxin-neutralizing immunoglobulin (Ig)A and IgG antibodies were found in pulmonary lavage concentrates and were accompanied by increased serum toxin neutralization titers.
mesh_terms: D000336:Aerosols; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D002417:Cattle / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D005098:Exotoxins / Q000276:immunology*; D007070:Immunoglobulin A / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D008168:Lung / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D009500:Neutralization Tests; D010325:Pasteurella / Q000276:immunology*; D007507:Therapeutic Irrigation / Q000662:veterinary
chemical_list: D000336:Aerosols; D000907:Antibodies, Bacterial; D005098:Exotoxins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; C018579:leukotoxin