DNA fragmentation by N-nitrosodimethylamine and methyl methanesulfonate in human hepatocyte primary cultures.
The ability of N-nitrosodimethylamine (DMN) and methyl methanesulfonate (MMS) to induce DNA damage in primary cultures of human hepatocytes was examined by the alkaline elution technique. Both the agents induced a dose-dependent increase in DNA elution rate, but appreciable differences in the degree of response to the procarcinogen DMN were observed among cultures obtained from the livers of four patients. A comparative analysis of DNA fragmentation indicated a substantial similarity between human and concurrently studied rat hepatocytes in their response to both DMN and MMS.
D000368:Aged; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000235:genetics; D004128:Dimethylnitrosamine / Q000633:toxicity*; D005260:Female; D006801:Humans; D008099:Liver / Q000187:drug effects*; D008297:Male; D008741:Methyl Methanesulfonate / Q000633:toxicity*; D008875:Middle Aged; D009152:Mutagenicity Tests; D009154:Mutation / Q000187:drug effects*
D004247:DNA; D008741:Methyl Methanesulfonate; D004128:Dimethylnitrosamine