Solubilization of hen brain neurotoxic esterase in dimethylsulfoxide.
Neurotoxic esterase is the putative site of initiation of organophosphorus-induced neuropathy. The protein is membrane-associated and will thus require solubilization before it can be purified. Its enzymic activity was retained in hen brain microsomes suspended in 10-60% (v/v in water) dimethylsulfoxide and 5-20% dimethylacetamide, but lost in 5-20% 1- and 2-propanol as well as higher concentrations of dimethylsulfoxide. Soluble activity (100,000 x g, 60 min supernatant) was not obtained with dimethylacetamide, but 24% of the recovered activity (67%) was solubilized in 40% dimethylsulfoxide, with retention of its native response to inhibitors. Solvent extraction of active enzyme is of intrinsic interest and expands the options for its purification.
D000433:1-Propanol / Q000494:pharmacology; D000081:Acetamides / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002645:Chickens; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005260:Female; D007700:Kinetics; D008861:Microsomes / Q000201:enzymology; D012995:Solubility
D000081:Acetamides; D000433:1-Propanol; D002265:Carboxylic Ester Hydrolases; C021859:neurotoxic esterase; C013959:dimethylacetamide; D004121:Dimethyl Sulfoxide