Perturbation of differentiated functions during viral infection in vivo. II. Viral reassortants map growth hormone defect to the S RNA of the lymphocytic choriomeningitis virus genome.
Lymphocytic choriomeningitis virus (LCMV) Armstrong caused disordered growth and glucose metabolism secondary to growth hormone deficiency in infected C3H/St mice. In contrast, LCMV strain WE replicated in fewer growth hormone-producing cells, failed to disrupt growth hormone synthesis, and did not cause growth hormone-induced disease in infected, matched controls. The generation of genetic reassortant viruses between the virulent strain of LCMV:Armstrong and the avirulent strain, LCMV:WE, is reported. Using such reassortants and both parental strains of virus in C3H/St mice, the perturbation of growth hormone synthesis was clearly mapped to the S RNA segment of LCMV:ARM.
D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D006224:Cricetinae; D005838:Genotype; D013006:Growth Hormone / Q000172:deficiency*; D007668:Kidney; D008216:Lymphocytic Choriomeningitis / Q000382:microbiology* / Q000473:pathology / Q000503:physiopathology; D008217:Lymphocytic choriomeningitis virus / Q000235:genetics* / Q000472:pathogenicity; D051379:Mice; D008809:Mice, Inbred C3H; D010902:Pituitary Gland / Q000382:microbiology / Q000473:pathology; D012367:RNA, Viral / Q000235:genetics*; D013045:Species Specificity
D012367:RNA, Viral; D013006:Growth Hormone