Chain-length specificities of mitochondrial and peroxisomal beta-oxidation of fatty acids in livers of rainbow trout (Salmo gairdneri).
Peroxisomes and mitochondria were prepared from livers of rainbow trout fed diets containing either 15% crude fish oil (CFO) or 11.5% partially hydrogenated fish oil (PHFO) plus 3.5% CFO. Peroxisomal preparations from the two dietary groups showed similar rates and substrate specificity patterns for acyl-CoA oxidation. The peroxisomal oxidation rate was highest with 12:0-CoA and decreased with increasing chain length, being negligible with 22-carbon acyl-CoA's. The trans isomer of 18:1(n-9) was oxidized at a higher rate than the cis isomer only by peroxisomes from the PHFO + CFO group. Mitochondria prepared from both groups of fish exhibited a broad chain-length specificity for the oxidation of acylcarnitines. Both polyunsaturated and trans monoenoic fatty acids were readily oxidized.
D000214:Acyl Coenzyme A / Q000378:metabolism; D000818:Animals; D002331:Carnitine / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008830:Microbodies / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D010084:Oxidation-Reduction; D013329:Structure-Activity Relationship; D014337:Trout
D000214:Acyl Coenzyme A; D005227:Fatty Acids; D002331:Carnitine