[Use of cell enucleation in studying the stability of cytoplasmic organelles and cytoplasm organization].
A study was made of the viability and ultrastructure of cytoplasts produced by enucleation of cytochalasin-induced A9 cells in suspension. These cytoplasts are in general as viable as cells enucleated in the monolayer. The organization of the cytoplasm, i.e. a specific distribution of cytoplasmic organelles, is conserved for at least 24 hours in the absence of the nucleus. This fact may reflect a high degree of autonomy of the cytoskeleton.
D000818:Animals; D001345:Autoradiography; D002467:Cell Nucleus / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D003571:Cytochalasin B / Q000494:pharmacology; D003593:Cytoplasm / Q000187:drug effects / Q000648:ultrastructure*; D003599:Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure; D005362:Ficoll / Q000494:pharmacology; D007739:L Cells / Q000187:drug effects / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D009940:Organoids / Q000187:drug effects / Q000648:ultrastructure*; D013997:Time Factors
D005362:Ficoll; D003571:Cytochalasin B
Primenenie énukleatsii kletok dlia izucheniia stabil'nosti tsitoplazmaticheskikh organell i organizatsii tsitoplazmy.