Cholesterol esterification in human secretory endometrium and in endometrial cancer tissue. Demonstration of microsomal acyl-CoA-cholesterol acyl-transferase (ACAT) activity.
Human secretory endometrium and endometrial cancer tissue both contain acyl-CoA:cholesterol-acyltransferase (ACAT). The optimal assay conditions for measuring ACAT activity in microsomes prepared from these tissues were determined. The activity in normal secretory endometrium was 15.3 +/- 1.27 pmol cholesteryl ester formed per mg protein-1 per min-1, whereas 3 out of 4 endometrial cancers had 3-7-fold higher ACAT activity.
D000328:Adult; D000368:Aged; D002784:Cholesterol / Q000378:metabolism*; D004717:Endometrium / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D004951:Esterification; D005260:Female; D006801:Humans; D008597:Menstrual Cycle; D008861:Microsomes / Q000201:enzymology*; D002785:Sterol O-Acyltransferase / Q000378:metabolism*; D014594:Uterine Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology
D002784:Cholesterol; D002785:Sterol O-Acyltransferase