Deep freezing of horse embryos.
Fourteen horse embryos recovered non-surgically on Days 6-8 after ovulation (Day 0) were cooled slowly to - 35 degrees C (7 embryos) or - 40 degrees C (7 embryos) and stored in liquid nitrogen (- 196 degrees C) for 4-98 days. Surgical transfer of the thawed embryos to unmated recipient mares that had ovulated - 2 to + 1 days with respect to the embryo donors resulted initially in the establishment of 4 conceptuses. However, only one mare maintained her pregnancy to term.
D000818:Animals; D001755:Blastocyst; D002470:Cell Survival; D004624:Embryo Transfer; D005260:Female; D005615:Freezing; D006736:Horses / Q000196:embryology*; D011247:Pregnancy; D014021:Tissue Preservation / Q000662:veterinary*