Amphiphilic cationic peptides mediate cell adhesion to plastic surfaces.
Four amphiphilic peptides, each with net charges of +2 or more at neutrality and molecular weights under 4 kilodaltons, were found to mediate the adhesion of normal rat kidney fibroblasts to polystyrene surfaces. Two of these peptides, a model for calcitonin (peptide 1, MCT) and melittin (peptide 2, MEL), form amphiphilic alpha-helical structures at aqueous/nonpolar interfaces. The other two, a luteinizing hormone-releasing hormone model (peptide 3, LHM) and a platelet factor model (peptide 4, MPF) form beta-strand structures in amphiphilic environments. Although it contains only 10 residues, LHM mediated adhesion to surfaces coated with solutions containing as little as 10 pmoles/ml of peptide. All four of these peptides were capable of forming monolayers at air-buffer interfaces with collapse pressures greater than 20 dynes/cm. None of these four peptides contains the tetrapeptide sequence Arg-Gly-Asp-Ser, which has been associated with fibronectin-mediated cell adhesion. Ten polypeptides that also lacked the sequence Arg-Gly-Asp-Ser but were nonamphiphilic and/or had net charges less than +2 at neutrality were all incapable of mediating cell adhesion (Pierschbacher and Ruoslahti, 1984). The morphologies of NRK cells spread on polystyrene coated with peptide LHM resemble the morphologies on fibronectin-coated surfaces, whereas cells spread on surfaces coated with MCT or MEL exhibit strikingly different morphologies. The adhesiveness of MCT, MEL, LHM, and MPF implies that many amphiphilic cationic peptides could prove useful as well defined adhesive substrata for cell culture and for studies of the mechanism of cell adhesion.
D000595:Amino Acid Sequence; D000818:Animals; D001514:Bee Venoms / Q000494:pharmacology*; D002116:Calcitonin / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects*; D002942:Circular Dichroism; D007668:Kidney / Q000166:cytology; D007986:Luteinizing Hormone / Q000494:pharmacology*; D008555:Melitten / Q000494:pharmacology*; D008970:Molecular Weight; D010446:Peptide Fragments / Q000494:pharmacology*; D010969:Plastics; D010978:Platelet Factor 4 / Q000494:pharmacology*; D051381:Rats; D013329:Structure-Activity Relationship
D001514:Bee Venoms; D010446:Peptide Fragments; D010969:Plastics; D008555:Melitten; D010978:Platelet Factor 4; D007986:Luteinizing Hormone; D002116:Calcitonin