Substrates for the attachment of adult cardiac myocytes in culture.
To achieve early cellular attachment of isolated adult cardiac myocytes in primary culture, various procedures were tested. A selective attachment of rod-shaped cells can be obtained either on serum-preincubated tissue-culture plastic dishes, or on modified plastic materials to which serum proteins are bound covalently, or on laminin. For the pretreatment of tissues culture dishes fetal calf serum is better suited than other sera.
D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D005312:Fetal Blood; D006168:Guinea Pigs; D006801:Humans; D009206:Myocardium / Q000166:cytology*; D010969:Plastics; D011137:Polystyrenes; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000378:metabolism
D003470:Culture Media; D010969:Plastics; D011137:Polystyrenes; D012709:Serum Albumin