Isolation and characterization of glycosaminoglycans from Schistosoma mansoni.
Tegumental tissues of paired adult Schistosoma mansoni were removed by treatment with Triton X-100 and recovered by centrifugation. The chloroform-methanol insoluble residues of this isolated tegumental fraction and of the denuded carcasses were analysed for glycosaminoglycan (GAG) and sialic acid contents. Treatment with GAG-specific enzymes followed by electrophoretic analysis showed that both the carcass and tegument contained heparin and/or heparan sulfate, chondroitin sulfate and hyaluronic acid. All these except hyaluronic acid were present in the tegumental fraction. Based on uronic acid content, about 73% of the total GAG was in the tegumental membrane, 15% in the tegmental matrix and the remaining 12% was in the carcass. The presence of heparin-like polysaccharide may present entrapment of the schistosoma by the hosts' blood-clotting process.
D000818:Animals; D002810:Chondroitinases and Chondroitin Lyases; D006224:Cricetinae; D006025:Glycosaminoglycans / Q000097:blood / Q000302:isolation & purification*; D019763:Heparin Lyase; D006821:Hyaluronoglucosaminidase; D008297:Male; D008647:Mesocricetus; D011133:Polysaccharide-Lyases; D012550:Schistosoma mansoni / Q000032:analysis*; D013045:Species Specificity
D006025:Glycosaminoglycans; D006821:Hyaluronoglucosaminidase; D002810:Chondroitinases and Chondroitin Lyases; D011133:Polysaccharide-Lyases; D019763:Heparin Lyase