Antagonism of 2-5 A-mediated inhibition of protein synthesis in intact cells by 2',5'-(pA)3.
In vitro studies have shown that the translational inhibitory activity of 2-5 A can be blocked by the oligoribonucleotide 2',5'-(pA)3. We have examined the effect of simultaneous introduction of inhibitor and antagonist into intact mouse cells using calcium phosphate coprecipitation. Upon introduction of 10(-4) M 2',5'-(pA)3 and 10(-6) M 2-5 A, inhibition of protein synthesis was prevented. Efficiency of calcium phosphate precipitation of 2-5 A in the presence or absence of 2',5'-(pA)3 was comparable. Introduction of 2',5'-(pA)3 analogs showed that nucleotides which do not bind well to the 2-5 A dependent endonuclease do not prevent 2-5 A inhibitory activity. Thus, 2',5'-(pA)3 functions as an antagonist of 2-5 A in vivo.
D000227:Adenine Nucleotides / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D000818:Animals; D007739:L Cells / Q000378:metabolism; D051379:Mice; D009843:Oligoribonucleotides / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D014176:Protein Biosynthesis
D000227:Adenine Nucleotides; D009843:Oligoribonucleotides; C023505:2',5'-oligoadenylate