Study of human chromosomes. IV. Labeling of chromosomal proteins with the amino group specific fluorescent reagent fluorescamine.
We describe a method for labeling chromosomal proteins with an amino-group-specific fluorescent reagent, fluorescamine. Chromosomes thus labeled appear either as uniformly fluorescent or as haloes in structure depending on the proteins remaining after treatment with acid-alcohol fixation. Using fluorescamine as a probe, we demonstrate that there is a substantial loss of labeled proteins during the chromosomal preparation and also during the trypsin treatment used in the banding of chromosomes.
D000650:Amnion / Q000166:cytology; D002871:Chromosome Banding; D002877:Chromosomes, Human / Q000648:ultrastructure*; D005260:Female; D005450:Fluorescamine; D006801:Humans; D007621:Karyotyping; D008214:Lymphocytes / Q000166:cytology; D008677:Metaphase; D008856:Microscopy, Fluorescence / Q000379:methods; D009698:Nucleoproteins / Q000032:analysis*; D011247:Pregnancy; D014357:Trypsin
D009698:Nucleoproteins; D005450:Fluorescamine; D014357:Trypsin