Determination of the polyamine content of rat heart mitochondria by the use of heparin-sepharose.
Heparin-sepharose has been utilized to remove polyamines adsorbed to the cytoplasmic surface of rat heart mitochondria. The results obtained can be summarized as follows: Heparin-sepharose removes 90% of the spermine, 98% of the spermidine, and 98% of the putrescine adsorbed. Polyamine contents of chromatographed mitochondria amount to 2.66 and 0.36 nmol spermine and spermidine, respectively, per mg of mitochondrial protein.
D000818:Animals; D002852:Chromatography, Ion Exchange; D006493:Heparin; D066298:In Vitro Techniques; D008929:Mitochondria, Heart / Q000032:analysis*; D011073:Polyamines / Q000032:analysis*; D011700:Putrescine / Q000032:analysis; D051381:Rats; D012685:Sepharose; D013095:Spermidine / Q000032:analysis; D013096:Spermine / Q000032:analysis
D011073:Polyamines; D013096:Spermine; D006493:Heparin; D012685:Sepharose; D013095:Spermidine; D011700:Putrescine