Rapid protein determinations on ultrafiltrates prepared for free phenytoin quantification.
Ultrafiltration techniques for the separation of bound and free ligand are dependent on minimal amounts of protein-bound drug leakage through the membrane devices. A dye-impregnated test strip protein assay (Chemstrip-9) provides a rapid, visual assessment of membrane protein leakage. The test strips are sensitive to 200 mg/L of albumin in ultrafiltrates and do not react with as much as 4 mg/L of phenytoin.
D001798:Blood Proteins / Q000032:analysis*; D002648:Child; D006801:Humans; D010672:Phenytoin / Q000097:blood*; D011485:Protein Binding; D014462:Ultrafiltration
D001798:Blood Proteins; D010672:Phenytoin