Cloning of the cytotoxin-hemolysin gene of Vibrio vulnificus.
Genes encoding the cytotoxin-hemolysin of Vibrio vulnificus were cloned in Escherichia coli by using the lytic cloning vector, lambda 1059. Subcloning in plasmid pBR325 resulted in the isolation of a 3.2-kilobase DNA fragment containing the cytotoxin gene. By using this fragment as a DNA probe, homologous gene sequences were detected in all 54 V. vulnificus strains studied; homologous sequences were present in none of 96 isolates from 29 other bacterial species.
D000818:Animals; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D003603:Cytotoxins / Q000235:genetics*; D005260:Female; D006460:Hemolysin Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014733:Vibrio / Q000235:genetics*
D003603:Cytotoxins; D006460:Hemolysin Proteins