Assay of protein kinase C with an N-bromosuccinimide-cleavage fragment of histone H1.
N-Bromosuccinimide cleavage of a lysine-rich histone fraction (histone III-S) yields a peptide substrate, purified by reverse-phase h.p.l.c., for the Ca2+ + phospholipid-dependent protein kinase (protein kinase C). This substrate displays no reactivity with the cyclic AMP-dependent protein kinase, and may prove useful for the detection of protein kinase C activity in crude tissue extracts.
D000273:Adipose Tissue / Q000201:enzymology; D000818:Animals; D001974:Bromosuccinimide; D002851:Chromatography, High Pressure Liquid; D006657:Histones; D066298:In Vitro Techniques; D010446:Peptide Fragments / Q000378:metabolism*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism; D051381:Rats; D013388:Succinimides
D006657:Histones; D010446:Peptide Fragments; D013388:Succinimides; D011494:Protein Kinases; D011493:Protein Kinase C; D001974:Bromosuccinimide