title: [Tryptophan of facultative methylotrophic bacteria Pseudomonas sp. M. II. Regulation of tryptophan biosynthesis].
abstract: Regulation of tryptophan biosynthesis of facultative methylotrophic Pseudomonas sp. M was studied. Repression of the trpE, trpD and trpC genes by tryptophan was demonstrated. It was also shown that the trpE and trpDC genes are derepressed noncoordinately. No regulation of the trpF gene product could be demonstrated, indicating that its synthesis is constitutive. The trpA and trpB genes are inducible by indol-3-glycerophosphate. Anthranilate synthase and tryptophan synthase were sensitive to the feedback inhibition. The tryptophan concentrations giving 50% inhibition were estimated to be 9 microM and 1 microM, respectively. Experimental evidence for activation of the N-5-phosphoribosyl anthranilate isomerase and for inhibition of the indol-3-glycerophosphate synthase by some tryptophan intermediates was obtained.
mesh_terms: D000878:Anthranilate Synthase / Q000037:antagonists & inhibitors; D004789:Enzyme Activation; D004790:Enzyme Induction; D005809:Genes, Regulator; D009154:Mutation; D009876:Operon; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D014364:Tryptophan / Q000096:biosynthesis* / Q000235:genetics; D014367:Tryptophan Synthase / Q000037:antagonists & inhibitors
chemical_list: D014364:Tryptophan; D000878:Anthranilate Synthase; D014367:Tryptophan Synthase
vernacular_title: Triptofanovyĭ operon fakul'tativnykh metilotrofnykh bakteriĭ Pseudomonas sp. M. Soobshchenie II. Reguliatsiia biosinteza triptofana.