The effect of reserpine treatment on the extraneuronal uptake of [3H]-isoprenaline into rat atria.
Treatment of rats with reserpine (1 mg kg-1 day-1) for up to 7 days resulted in a marked decrease in a corticosterone-sensitive component of the extraneuronal accumulation of [3H]-isoprenaline into their atria. The change in extraneuronal uptake did not appear to be due to a direct action of reserpine on the uptake mechanism, since it was several days before the treatment had a significant effect on the accumulation of [3H]-isoprenaline. Further, reserpine in vitro did not inhibit extraneuronal uptake. The reserpine-induced change in the accumulation of [3H]-isoprenaline was not an artifact due to changes in water balance, ion distribution, extracellular space or tissue atrophy. Nor was the change due to an increase in the efflux of [3H]-isoprenaline from the tissue. These experiments support the suggestion that the extraneuronal uptake is dependent upon a functional adrenergic innervation.
D000818:Animals; D001834:Body Water / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D003042:Cocaine / Q000494:pharmacology; D003345:Corticosterone / Q000494:pharmacology; D004573:Electrolytes / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism; D006325:Heart Atria / Q000378:metabolism; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012110:Reserpine / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence; D013997:Time Factors
D004573:Electrolytes; D012110:Reserpine; D003042:Cocaine; D007545:Isoproterenol; D003345:Corticosterone