Structures of deepoxytrichothecene metabolites from 3'-hydroxy HT-2 toxin and T-2 tetraol in rats.
3'-Hydroxy HT-2 toxin and T-2 tetraol, in vivo metabolites of T-2 toxin, were orally administered to Wistar rats, and four metabolites having a trichothec-9,12-diene nucleus, which were termed deepoxytrichothecenes, were newly found in the excreta. Their structures were confirmed as 3'-hydroxy-deepoxy HT-2, 3'-hydroxy-deepoxy T-2 triol, 15-acetyl-deepoxy T-2 tetraol, and deepoxy T-2 tetraol on the basis of mass and nuclear magnetic resonance spectroscopy. Resolution of T-2 metabolites and corresponding deepoxytrichothecenes by gas-liquid and thin-layer chromatography was also described.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D005243:Feces / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013058:Mass Spectrometry; D051381:Rats; D011919:Rats, Inbred Strains; D012717:Sesquiterpenes / Q000378:metabolism*; D013605:T-2 Toxin / Q000031:analogs & derivatives / Q000378:metabolism*; D014255:Trichothecenes / Q000378:metabolism*
D012717:Sesquiterpenes; D014255:Trichothecenes; C046700:T-2 tetraol; C049185:3'-hydroxy HT-2 toxin; D013605:T-2 Toxin