High-performance liquid chromatographic separation and determination of 2-(diethylamino)ethyl diphenylpropylacetate and its metabolites from biological samples.
A high-performance liquid chromatographic method for the determination of 2-(diethylamino)ethyl diphenylpropylacetate (SKF 525-A; Proadifen), its two deethylated metabolites and a hydrolytic metabolite in biological samples has been developed. From many solvent systems investigated the compounds are best separated on an ODS/Sil-X column using an acetonitrile-phosphate buffer system containing 0.2% decylamine. The use of acetonitrile overcomes limitations associated with previously published methods for separating basic compounds where decylamine was used in a methanolic eluent. With the sampling procedures used, overall recoveries from biological tissues were around 80%. The chromatographic system also separates three tricyclic antidepressants which can be used as internal standards.
D000818:Animals; D000929:Antidepressive Agents, Tricyclic / Q000032:analysis; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D008099:Liver / Q000032:analysis; D011335:Proadifen / Q000032:analysis* / Q000378:metabolism; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
D000929:Antidepressive Agents, Tricyclic; D011335:Proadifen