Role of the fungal cell wall in the granulomatous response of mice to the agents of chromomycosis.
Mice were given, intraperitoneally, inocula of a cell-wall preparation and fractions thereof from Fonsecaea pedrosoi, F. compactum, Cladosporium carrioni and Phialophora verrucosum. Large doses of cell-wall preparation, with or without trypsin treatment, produced a pronounced loss of body weight, a granulomatous reaction and, sometimes, death. After extraction of the cell wall preparation with 1N NaOH, three fractions were obtained: an alkali-insoluble fraction 1; an alkali-soluble acid-insoluble fraction 2; and an alkali- and acid-soluble fraction 3. Intravenous administration showed that only fraction 1 induced a granulomatous reaction and death in mice.
D000818:Animals; D002473:Cell Wall / Q000502:physiology; D002862:Chromoblastomycosis / Q000382:microbiology*; D002963:Cladosporium / Q000472:pathogenicity / Q000648:ultrastructure; D006099:Granuloma / Q000382:microbiology*; D008172:Lung Diseases, Fungal / Q000382:microbiology*; D051379:Mice; D003904:Mitosporic Fungi / Q000472:pathogenicity* / Q000648:ultrastructure; D010676:Phialophora / Q000472:pathogenicity* / Q000648:ultrastructure; D014357:Trypsin / Q000494:pharmacology; D014774:Virulence
D014357:Trypsin