title: The effects of the organophosphate insecticide malathion on very young chick embryos: malformations detected by histological examination.
abstract: This histological study sought to determine the nature and incidence of developmental abnormalities induced by one of the reportedly least teratogenic of insecticides injected into very young chick embryos. Using techniques to assure rapid contact between injectant and embryo, eggs incubated for 24, 48, or 72 hr were injected with corn oil or 125 micrograms-4.0 mg malathion. The embryos were recovered 48 hr later, paraffin-embedded, serially cross-sectioned, and examined in detail. Structures affected (and the nature of the defects) were as follows: wing level notochord and spinal cord (folded or undulated); trunk/leg level spinal cord (variously, neural folds unfused, roof infolded, canal partitioned, etc.); eye (lens misshapen or severely thinned, optic cup incompletely invaginated); diencephalon (epiphysis bifurcated or off-center, supernumerary outgrowths); cardiovascular structures (atrium and major blood vessels enlarged); and tailbud (curled into hindgut: ourentery). Overall incidence was both dose- and age-related, doubling for each doubling of dose and tripling for each 24 hr less age at exposure. For most (not all) individual structures, incidence was greatest when exposed at 24 hr and nil at 72 hr. Severity of effect was not consistently dose- or age-dependent. We conclude that contrary to previous reports, 24- to 72-hr embryos are highly vulnerable to insecticide exposure, with the youngest the most vulnerable, and many of the defects detected may be attributed to either of two mechanisms: failure in formation of the supportive sheath, or factors that cause epithelial morphogenesis (e.g., microtubules, microfilaments, extracellular material, cell-to-cell adhesion mechanisms). Previous observations that 1- to 3-day embryos are relatively unresponsive to insecticides are probably artifactual owing to imprecise techniques.
mesh_terms: D000014:Abnormalities, Drug-Induced / Q000473:pathology*; D000818:Animals; D002319:Cardiovascular System / Q000187:drug effects / Q000196:embryology; D002448:Cell Adhesion; D002642:Chick Embryo; D004027:Diencephalon / Q000187:drug effects / Q000196:embryology; D004305:Dose-Response Relationship, Drug; D005123:Eye / Q000187:drug effects / Q000196:embryology; D008294:Malathion / Q000494:pharmacology*; D009024:Morphogenesis; D013116:Spinal Cord / Q000187:drug effects / Q000196:embryology; D013623:Tail / Q000187:drug effects / Q000196:embryology; D013997:Time Factors
chemical_list: D008294:Malathion