Detection of blood group type glycosphingolipid antigens on thin-layer plates using polyclonal antisera.
The conditions for binding of antibodies to glycosphingolipids separated on a thin-layer plate have been optimized for polyclonal antisera. The method has a broad detection range with low background staining. Examples are shown for the detection of blood group A and B active glycosphingolipids.
D000906:Antibodies; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods; D004912:Erythrocytes / Q000032:analysis / Q000276:immunology; D006028:Glycosphingolipids / Q000097:blood / Q000276:immunology*; D006801:Humans; D007519:Isoantigens / Q000032:analysis*
D000906:Antibodies; D006028:Glycosphingolipids; D007519:Isoantigens