Effects of synthetic blood and crystalloid cardioplegic solutions on coronary endothelium: an experimental scanning electron microscope study.
In an experimental study the effects of Fluosol DA (added with potassium chloride) on the vascular interface and endothelial cells were compared to those of crystalloid potassium cardioplegic solution using scanning electron microscope. Twenty rabbits (10 in each group) were sacrificed, the hearts with ascending aorta were immediately excised, and cold oxygenated solution was infused via a cannula inserted into the cross-clamped aorta. The hearts were left immersed in the perfusion medium for 2 hr. In the Fluosol DA group endothelial cover and endothelial cells were normal or minimal changes were seen in seven cases. Occasional breaking of intercellular attachments, small areas of denuded flow surface, and disappearance of microvilli were seen in three cases. In the crystalloid potassium cardioplegic group 7 of the 10 cases showed moderate or severe damage with large areas of denuded flow surface. The present experimental protocol represented an extreme situation where no collateral coronary blood was present. The coronary endothelial damage was obvious after the crystalloid potassium cardioplegic solution. Similar damage was not found following Fluosol DA infusion.
D000818:Animals; D001802:Blood Substitutes / Q000633:toxicity*; D003331:Coronary Vessels / Q000187:drug effects* / Q000648:ultrastructure; D004338:Drug Combinations / Q000633:toxicity; D004727:Endothelium / Q000187:drug effects / Q000648:ultrastructure; D005466:Fluorocarbons / Q000633:toxicity*; D006324:Heart Arrest, Induced / Q000379:methods*; D006895:Hydroxyethyl Starch Derivatives; D008855:Microscopy, Electron, Scanning; D011188:Potassium / Q000633:toxicity*; D017680:Potassium Compounds; D011817:Rabbits
D001802:Blood Substitutes; D004338:Drug Combinations; D005466:Fluorocarbons; D006895:Hydroxyethyl Starch Derivatives; D017680:Potassium Compounds; C018369:potassium cardioplegic solution; C017323:glucose, glycerol, hydroxyethyl starch, perfluorodecalin, perfluorotripropylamine, pluronic F-68, salts, yolk phospholipids drug combination; D011188:Potassium