The effect of nephrectomy and ureter ligation on plasma neurotensin-like immunoreactivity levels in the conscious rat.
The effect of nephrectomy and ureter ligation on the concentration of endogenous plasma neurotensin-like immunoreactivity (p-NTLI) was studied in conscious rats by means of antiserum 17-8201 which detects NT (1-13) only, and antiserum 0-7709 which detects NT(1-13) and NT(1-8). The unstimulated p-NTLI concentration did not change significantly during a 25-h observation period following nephrectomy in comparison with sham operation. However, stimulation of the release of NTLI by intraduodenal administration of oleic acid (0.2 ml) resulted in significantly higher p-NTLI levels in the nephrectomized rats than in the sham operated rats. Ureter ligation did not significantly affect basal or stimulated p-NTLI. The data indicate that the kidneys play an important part in the elimination of p-NTLI released after fat ingestion.
D000818:Animals; D003243:Consciousness; D004041:Dietary Fats / Q000494:pharmacology; D007106:Immune Sera; D007668:Kidney / Q000502:physiology* / Q000601:surgery; D008026:Ligation; D008297:Male; D009392:Nephrectomy; D009419:Nerve Tissue Proteins / Q000097:blood*; D009479:Neuropeptides; D009496:Neurotensin / Q000097:blood* / Q000378:metabolism; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical; D013997:Time Factors; D014513:Ureter / Q000502:physiology* / Q000601:surgery
D004041:Dietary Fats; D007106:Immune Sera; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D009829:Oleic Acids; C031644:neurotensin-like immunoreactivity; D019301:Oleic Acid; D009496:Neurotensin