title: Reconstitution of a porcine submaxillary gland beta-D-galactoside alpha 2----3 sialyltransferase into liposomes.
abstract: Form A of the beta-D-galactoside alpha 2----3 sialyltransferase from porcine submaxillary glands was incorporated into liposomes. Incorporation was achieved by gel filtration of the enzyme in the presence of octylglucoside-phospholipid micelles. As detergent was removed during gel filtration, liposomes (average diameter, 370 A) with bound enzyme were formed and emerged unretarded from the column. The recovery of enzyme activity in the liposomes was about 40% of the initial activity starting with as little as 9 micrograms of transferase. Chromatography on Sepharose CL6B and sucrose density gradient centrifugation confirmed the association of enzyme with liposomes. In contrast to Form A, Form B of the sialyltransferase, which lacks the proposed lipid-binding domain of Form A, cannot be incorporated into liposomes. Form A of the transferase was also incorporated into liposomes composed of phosphatidylcholine, cholesterol, and a mixture of phospholipids from the membranes of the Golgi apparatus from porcine submaxillary glands. Although the transferase was distributed about equally on the internal and external surface of the phosphatidylcholine liposomes, most of the transferase was on the external surface in liposomes containing cholesterol (72%) or in liposomes containing Golgi apparatus phospholipids (88%). The enzyme bound to phosphatidylcholine liposomes was shown by kinetic analysis to have the same activity as that found in the presence of activity-stimulating detergents such as Triton X-100. Enzyme incorporated into cholesterol-containing liposomes had the same activity. In contrast, enzyme bound to liposomes formed from the Golgi apparatus mixed phospholipids had a lower activity, but one similar to that of the transferase in Golgi apparatus membranes. These studies suggest that the composition of a biological membrane may well influence the orientation of the transferase in the membrane as well as modulate its enzymic activity.
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient; D002784:Cholesterol; D002850:Chromatography, Gel; D005960:Glucosides; D006056:Golgi Apparatus / Q000502:physiology; D007425:Intracellular Membranes / Q000502:physiology; D007700:Kinetics; D008081:Liposomes / Q000378:metabolism*; D008563:Membrane Lipids / Q000502:physiology; D008823:Micelles; D008854:Microscopy, Electron; D017830:Octoxynol; D010713:Phosphatidylcholines; D010743:Phospholipids / Q000502:physiology; D011092:Polyethylene Glycols / Q000494:pharmacology; D012799:Sialyltransferases / Q000378:metabolism*; D013363:Submandibular Gland / Q000201:enzymology*; D013552:Swine; D014166:Transferases / Q000378:metabolism*; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase
chemical_list: D005960:Glucosides; D008081:Liposomes; D008563:Membrane Lipids; D008823:Micelles; D010713:Phosphatidylcholines; D010743:Phospholipids; C018619:octyl-beta-D-glucoside; D011092:Polyethylene Glycols; D017830:Octoxynol; D002784:Cholesterol; D014166:Transferases; D012799:Sialyltransferases; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase