Selective induction of manganous superoxide dismutase in human monocytes.
Radioimmunoassays for both human copper-zinc and manganous superoxide dismutases (Cu-Zn SOD and Mn SOD, respectively) have been developed, validated, and utilized to measure the concentrations of these enzymes in cultured monocytes. Monocyte Mn SOD increased 4.7-fold over basal during 3 days of culture, an increase that was markedly enhanced by stimulation with bacterial lipopolysaccharide (LPS). Cu-Zn SOD showed a transient decrease over the culture period but was unaffected by LPS. Stimulation with muramyl dipeptide had minimal effect on Mn SOD and no effect on Cu-Zn SOD during culture, even at a concentration capable of activating the monocytes, as defined by zymosan-induced superoxide production.
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000494:pharmacology; D004790:Enzyme Induction; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000201:enzymology*; D011863:Radioimmunoassay / Q000379:methods; D013268:Stimulation, Chemical; D013482:Superoxide Dismutase / Q000032:analysis; D015054:Zymosan / Q000494:pharmacology
D008070:Lipopolysaccharides; D000119:Acetylmuramyl-Alanyl-Isoglutamine; D015054:Zymosan; D013482:Superoxide Dismutase