Analysis of mercury ions binding to the smooth muscle of guinea-pig taenia coli.
Mercury uptake to taenia coli increased with an increase of the Hg2+ concentration (0.05-5 mmol/l). The Scatchard plots of mercury uptake showed that two qualitative Hg2+ binding sites (high and low affinity sites) exist in taenia coli. The Scatchard analysis of Hg2+ binding at 4 degrees C indicated a single class of binding site (high affinity binding site only). These results indicate that Hg2+ binds to high affinity binding sites of cell membrane and Hg2+ of low affinity binding sites may reflect intracellular accumulation in taenia coli.
D000818:Animals; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D003106:Colon / Q000378:metabolism; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D008628:Mercury / Q000378:metabolism* / Q000494:pharmacology; D009130:Muscle, Smooth / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
D008628:Mercury; D002118:Calcium