Extemporaneous preparation of large unilamellar liposomes.
Direct contact between lipids solubilized by octyl glucoside and Amberlite XAD-2 beads yielded large liposomes (240 nm diameter) with no residual detergent molecules, in less than 10 min. This extemporaneous preparation of liposomes was prepared with a detergent/bead ratio no higher than 0.12 (mumol/mg) and a phosphatidylcholine/phosphatidylserine/cholesterol molar ratio of 1:1:1. The liposomes were mainly unilamellar, as deduced from thin section and freeze-fracture electron micrographs and from measurement of calcein incorporation into the vesicles. The relatively large internal volume of these vesicles (8.9 l/mol lipid) accounts for the high percentage of entrapped material observed. The percentage increased with lipid concentration, but could not be increased above 20% corresponding to 20 mM total lipids.
D055598:Chemical Phenomena; D002621:Chemistry; D003902:Detergents; D005960:Glucosides; D006027:Glycosides; D007475:Ion Exchange Resins; D007700:Kinetics; D008081:Liposomes; D008854:Microscopy, Electron; D008954:Models, Biological; D011137:Polystyrenes; D013501:Surface-Active Agents
D003902:Detergents; D005960:Glucosides; D006027:Glycosides; D007475:Ion Exchange Resins; D008081:Liposomes; D011137:Polystyrenes; D013501:Surface-Active Agents; C018619:octyl-beta-D-glucoside; C003771:Amberlite XAD-2 resin