Studies on peptides. CXXVIII. Application of new heterobifunctional crosslinking reagents for the preparation of neurokinin (A and B)-BSA (bovine serum albumin) conjugates.
A decapeptide corresponding to the entire amino acid sequence of neurokinin A, a porcine spinal cord peptide, was synthesized in a conventional manner using protecting groups removable by 1 M TFMSA-thioanisole in TFA. The HS-CH2CH2CO group was introduced onto the synthetic neurokinin A by reaction of 3-(S-acetyl-thiopropionyl)-thiazolidine-2-thione, followed by deacetylation with hydroxylamine. 2,4-Dinitrophenyl-p-(beta-nitrovinyl)-benzoate trapped the above HS-CH2CH2CO-neurokinin A derivative in acidic media, then BSA in basic media in nearly quantitative yield. A similar decapeptide, neurokinin B, was also synthesized and conjugated onto BSA using an alternative SH-introducing reagent, 3-(S-p-methoxybenzyl-thiopropionyl)-thiazolidine-2-thione, and the above heterobifunctional conjugating reagent.
D000595:Amino Acid Sequence; D000818:Animals; D003432:Cross-Linking Reagents; D007202:Indicators and Reagents; D009419:Nerve Tissue Proteins; D015288:Neurokinin A; D015287:Neurokinin B; D012710:Serum Albumin, Bovine; D013116:Spinal Cord; D013329:Structure-Activity Relationship; D013552:Swine
D003432:Cross-Linking Reagents; D007202:Indicators and Reagents; D009419:Nerve Tissue Proteins; C049179:neurokinin A-bovine serum albumin conjugate; C049180:neurokinin B-bovine serum albumin conjugate; D012710:Serum Albumin, Bovine; D015288:Neurokinin A; D015287:Neurokinin B