Hepatic peroxisomal and mitochondrial fatty acid oxidation in the riboflavin-deficient rat.
The effects of riboflavin deficiency on hepatic peroxisomal and mitochondrial palmitoyl-CoA oxidation were examined in weanling Wistar-strain male rats. The specific activities of peroxisomal catalase and palmitoyl-CoA-dependent NAD+ reduction were not affected by up to 10 weeks of riboflavin deficiency. In contrast, the specific activity of mitochondrial carnitine-dependent palmitoyl-CoA oxidation was depressed by 75% at 10 weeks of deficiency. The amount of peroxisomal protein per g of liver was not affected by riboflavin deficiency, whereas, expressed per liver, both riboflavin-deficient and pair-fed controls showed decreased peroxisomal protein compared with controls fed ad libitum. Hepatic mitochondria, but not peroxisomes, were sensitive to riboflavin deficiency.
D000214:Acyl Coenzyme A / Q000378:metabolism*; D000818:Animals; D002374:Catalase / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D008830:Microbodies / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism; D009929:Organ Size; D010084:Oxidation-Reduction; D010171:Palmitoyl Coenzyme A / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012257:Riboflavin Deficiency / Q000378:metabolism*
D000214:Acyl Coenzyme A; D010171:Palmitoyl Coenzyme A; D002374:Catalase