Osteoclasts isolated in primary cell culture--a model to study conditional changes in vitro.
Osteoclasts have been isolated out of the bone marrow of laying hens kept on a low calcium diet for eight days. After purification the cells are cultured in a DMEM suspension. Development of the incubated single cells was observed up to 34 days and morphological changes are reported. No major difference could be found if the osteoclasts were kept on acrylic dishes or on gas permeable teflon dishes as a ground substrate.
D000818:Animals; D001854:Bone Marrow Cells; D002136:Calcium, Dietary / Q000008:administration & dosage; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D002645:Chickens; D005260:Female; D010010:Osteoclasts / Q000166:cytology*; D013997:Time Factors
D002136:Calcium, Dietary