title: Effects of the free radical generator paraquat on differentiation, superoxide dismutase, glutathione and inorganic peroxides in microplasmodia of Physarum polycephalum.
abstract: The herbicide paraquat was used to investigate the effects of oxidative stress on the spherulation of Physarum polycephalum microplasmodia. The responses of a white non-differentiating strain of Physarum were compared with those of a common yellow strain that readily spherulates in salts-only starvation medium. The addition of paraquat to the salts medium increased the specific activity of superoxide dismutase in both strains; it also induced an increase in the intracellular inorganic peroxide concentration in both strains. Glutathione concentration was higher in the paraquat-treated yellow strain than in the controls. Paraquat had no effect on glutathione concentration in white microplasmodia. Paraquat accelerated spherulation in yellow microplasmodia. The white microplasmodia responded to the herbicide by cleaving into structures similar to immature spherules; however, these structures were not viable. The results of this study support the hypothesis that free radicals are involved in cell state transitions.
mesh_terms: D005609:Free Radicals; D005978:Glutathione / Q000378:metabolism*; D007700:Kinetics; D010269:Paraquat / Q000494:pharmacology*; D010545:Peroxides / Q000378:metabolism*; D010804:Physarum / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D005609:Free Radicals; D010545:Peroxides; D013482:Superoxide Dismutase; D005978:Glutathione; D010269:Paraquat