Low-temperature-induced changes in intracellular fatty acid fluxes in Dunaliella salina.
Two-minute exposures to exogenous [14C]palmitic, [14C]oleic, or [14C]lauric acid differentially labeled the lipids of Dunaliella salina microsomes and chloroplasts. Changes in fatty acid desaturation and intracellular movement during a subsequent 16-h incubation in nonradioactive medium indicated a slow transfer of lipids into the chloroplast from other organelles. Since Dunaliella lacks the massive traffic of microsomally produced glycerolipids into chloroplast galactolipids that dominates chloroplast-microsome lipid relations in most plant cells, it affords a sensitive system for studying more subtle intracellular lipid fluxes. Lowering the culture temperature from 30 to 12 degrees C was more inhibitory toward glycerolipid biosynthesis in chloroplasts than in microsomes. The ability of Dunaliella chloroplasts to utilize microsomal lipids may be essential for their systematic acclimation to low temperature.
D002736:Chloroplasts / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003080:Cold Temperature; D005227:Fatty Acids / Q000378:metabolism*; D006017:Glycolipids / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D010944:Plants / Q000378:metabolism*; D013997:Time Factors
D005227:Fatty Acids; D006017:Glycolipids