[Biochemical, hematological and immunological criteria for assessing chronic T-2 mycotoxicosis in mice].
The authors studied the effect of a long-term intragastric administration to CBA X C57Bl/6 male mice of T-2 toxin in doses of 0.067 mg/kg bw a day (1/100 of the LD50) or 0.33 and 0.45 mg/kg a day (1/20 and 1/15 of the LD50) on the liver content of protein, cytochrome P-450 SH-glutathione and on the activity of 10 lysosomal and microsomal enzymes and glutathione transferase. A dose-dependent increase in the activity of lysosomal hydrolases and glutathione transferase localized in cytosol was revealed together with a fall in the activity of microsomal aniline hydroxylase, carboxyl esterase and epoxide hydrolase. Emphasis is laid on a dose-dependent reduction in the liver of nonsedimented activity of lysosomal enzymes. In T-2 mycotoxicosis, the most sensitive and the most stable parameter was the activity of lysosomal enzymes in blood serum. That activity also declined during the recovery period, namely 3 months after discontinuance of toxin administration. Both groups of mice showed a progressive decrease in the blood leukocyte count and lysozyme content, whereas in the spleen, there was a decrease in the number of antibody-forming cells. It is concluded that biochemical, hematological and hematological characteristics should be taken into consideration in evaluating the chronic action of T-2 toxin.
D000818:Animals; D000921:Antibody-Producing Cells / Q000187:drug effects / Q000276:immunology; D001769:Blood / Q000187:drug effects; D002908:Chronic Disease; D004305:Dose-Response Relationship, Drug; D007114:Immunization; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009145:Mushroom Poisoning / Q000276:immunology / Q000378:metabolism*; D012717:Sesquiterpenes / Q000506:poisoning*; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D013605:T-2 Toxin / Q000506:poisoning*
D012717:Sesquiterpenes; D013605:T-2 Toxin
Biokhimicheskie, gematologicheskie i immunologicheskie kriterii otsenki khronicheskogo T-2 mikotoksikoza u mysheĭ.