[Fluorescence of Tb3 complexes with metaphase chromosomes of mink fibroblasts].
The fluorescence of the Tb3+ complex with metaphase chromosomes of mink fibroblasts was studied. It was shown that the fluorescence intensity of this complex is 6.5 times as high as that of the Tb3+-native DNA complex. The fluorescence of the chromosome-Tb3+ complex is predominantly determined by chromosomal DNA. The high intensity of fluorescence may be due to partial disturbances in the secondary structure of DNA during folding of the metaphasic chromosome.
D000818:Animals; D002875:Chromosomes / Q000032:analysis*; D004277:DNA, Single-Stranded / Q000032:analysis; D005347:Fibroblasts / Q000166:cytology / Q000648:ultrastructure; D005453:Fluorescence; D008677:Metaphase; D008907:Mink; D009691:Nucleic Acid Denaturation; D051381:Rats; D013725:Terbium / Q000032:analysis*
D004277:DNA, Single-Stranded; D013725:Terbium
Fluorestsentsiia kompleksov Tb3+ s metafaznymi khromosomami fibroblastov norki.