[Determination of glycosylated albumin and its clinical significance in diabetes mellitus].
Assessment of diabetic glycaemic control by nonenzymatically glycosylated albumin (G-A) was investigated. Serum albumin was purified by affinity chromatography on Affi-Gel Blue. Using the purified serum albumin in normal and diabetic subjects, G-A was estimated by a colorimetric thiobarbituric acid (TBA) method and the results compared with the levels determined using aminophenyl boronic acid (PBA) affinity chromatography. There was an excellent correlation between the levels estimated by TBA method and PBA affinity chromatography method (r = 0.94). The levels of G-A increased in patients with poorly controlled diabetes mellitus compared to normals. There was a significant correlation (r = 0.84) between the G-A and HbA1 levels in 43 normal and 167 diabetic subjects. As an estimate of diabetic glycaemic control by G-A, the correlations between the G-A and mean fasting blood sugar (FBS) levels were studied. There was a higher correlation (r = 0.67) between G-A and the mean FBS within 2 weeks. On starting insulin therapy in 8 juvenile diabetic subjects, there was a different temporal relationship between the FBS, G-A and HbA1 levels. The G-A levels were significantly decreased at 1, 2, 3 and 4 weeks compared to the HbA1 levels. The present results indicate that the G-A may provide a valuable tool for assessing the mean blood sugar levels between shorter intervals, since the turnover of serum albumin is considerably faster than that of HbA1.
D001786:Blood Glucose / Q000032:analysis; D001897:Boronic Acids; D002846:Chromatography, Affinity / Q000379:methods; D003124:Colorimetry; D003920:Diabetes Mellitus / Q000097:blood*; D017127:Glycation End Products, Advanced; D006801:Humans; D012709:Serum Albumin / Q000032:analysis*; D013858:Thiobarbiturates; D000093423:Glycated Serum Albumin
D001786:Blood Glucose; D001897:Boronic Acids; D017127:Glycation End Products, Advanced; D012709:Serum Albumin; D013858:Thiobarbiturates; C028592:3-aminobenzeneboronic acid; C029684:thiobarbituric acid; D000093423:Glycated Serum Albumin