title: Mechanism of the stimulation of respiration by fatty acids in rat liver.
abstract: The mechanism of stimulation of hepatic respiration by fatty acids was studied in isolated rat hepatocytes. Stimulation of respiration by fatty acids varied from about 35% to about 105% depending on chain length. The stimulatory effect of octanoate (1 mM) or oleate (0.5 mM) was prevented by oligomycin (2 micrograms/ml). With carboxyatractyloside (100 microM) and ouabain (2 mM) the stimulation of respiration was partially inhibited (by 50-70 and 50-60%, respectively). From these results it can be concluded that the increased rate of respiration after addition of fatty acids is coupled to ATP synthesis. A large part (50-60%) of this ATP is utilized by the (Na+ + K+)-ATPase.
mesh_terms: D019297:2,4-Dinitrophenol; D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D001278:Atractyloside / Q000031:analogs & derivatives / Q000494:pharmacology; D002210:Caprylates / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology; D004734:Energy Metabolism; D005227:Fatty Acids / Q000494:pharmacology*; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology; D009840:Oligomycins / Q000494:pharmacology; D010042:Ouabain / Q000494:pharmacology; D010101:Oxygen Consumption / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000227:Adenine Nucleotides; D002210:Caprylates; D004140:Dinitrophenols; D005227:Fatty Acids; D009829:Oleic Acids; D009840:Oligomycins; D001278:Atractyloside; D019301:Oleic Acid; D010042:Ouabain; C031492:octanoic acid; D019297:2,4-Dinitrophenol; C003853:carboxyatractyloside