title: Colony growth and self renewal of plasma cell precursors in multiple myeloma.
abstract: Culture conditions that support the growth of multi-and single-lineage hemopoietic colonies are also able to give rise to large myeloma colonies from bone marrow and peripheral blood samples of some patients with multiple myeloma. The culture system was used to determine the frequency of hemopoietic precursors and clonogenic myeloma progenitors in 71 patients with multiple myeloma studied in various clinical phases of the disease. The frequency of normal hemopoietic precursors in patients with benign monoclonal gammopathy and smoldering myeloma were indistinguishable from normal controls. Myeloma colonies were not observed in these subgroups. In contrast, patients with active disease showed significantly reduced hemopoietic colony formation, even before the initiation of therapy. A further reduction was demonstrated for patients with acute phase disease. A correlation between the frequency of hemopoietic colonies and the concentration of plasma cells in the plated sample was not observed. Large myeloma colonies with recloning potential were identified in cultures of specimens derived from 14 of the studied patients. These colonies were most frequently (ten cases) obtained from patients who had entered the acute phase of the disease. These patients manifested marrow failure (pancytopenia) and their marrow had a limited capacity to generate normal hemopoietic colonies. Three of the patients that formed myeloma colonies were studied in chronic phase following chemotherapy and one patient was examined at diagnosis. The myeloma colonies were composed exclusively of cells characterized by the same M protein as the patient. Some of the cells within the colonies retained their ability to self renew extensively, as demonstrated by serial recloning studies. Colonies derived from six of the patients are now propagated in semisolid and liquid medium for as many as nine to 34 generations. Patients that form myeloma colonies under these culture conditions represent a high-risk group with significantly shorter survival than patients not able to give rise to myeloma colonies. A Cox proportional hazards model was fitted to the data to determine the prognostic role of myeloma colony growth in culture after accounting for the influence of other well established risk factors, such as concentration of plasma cells and disease status. The analysis indicated that myeloma colony growth in culture serves as a strong and independent predictive indicator of poor clinical prognosis.
mesh_terms: D000206:Actuarial Analysis; D000368:Aged; D001853:Bone Marrow / Q000473:pathology; D002455:Cell Division; D002460:Cell Line; D002999:Clone Cells; D003114:Colony-Forming Units Assay; D005260:Female; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008297:Male; D009101:Multiple Myeloma / Q000097:blood / Q000473:pathology* / Q000628:therapy; D009194:Myeloma Proteins / Q000032:analysis; D010198:Pancytopenia / Q000473:pathology; D010950:Plasma Cells / Q000473:pathology*; D011379:Prognosis; D012306:Risk
chemical_list: D006023:Glycoproteins; D007070:Immunoglobulin A; D007075:Immunoglobulin M; D009194:Myeloma Proteins; C023587:protein M (glycoprotein)