The synthesis of mannose 1-phosphate in brain.
The interconversion of mannose-6-P and mannose-1-P in brain has been shown to be catalyzed by a distinct enzyme. The enzyme has been separated from most of the phosphoglucomutase activity of the brain. The residual phosphoglucomutase activity (less than 1%) may be associated with phosphomannomutase itself. Mannose-1,6-P2 or glucose-1,6-P2 is required for the reaction as well as a divalent cation (Mg2+ greater than Co2+ greater than Ni2+ greater than Mn2+). Glucose-1-P, glucose-6-P, and 2-deoxyglucose-6-P are also substrates or inhibitors. Other phosphorylated sugars tested, glucosamine-6-P, N-acetylglucosamine-6-P, galactose-6-P, fructose-6-P, ribose-5-P, and arabinose-5-P, do not affect the rate of the reaction when assayed in the presence of mannose-6-32P.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002238:Carbohydrate Epimerases / Q000378:metabolism*; D002848:Chromatography, DEAE-Cellulose; D019298:Glucose-6-Phosphate; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism*; D005958:Glucosephosphates / Q000494:pharmacology; D006600:Hexosephosphates / Q000096:biosynthesis*; D007700:Kinetics; D008359:Mannose-6-Phosphate Isomerase / Q000378:metabolism*; D008360:Mannosephosphates / Q000096:biosynthesis*; D010766:Phosphorylation; D011817:Rabbits
D005958:Glucosephosphates; D006600:Hexosephosphates; D008360:Mannosephosphates; C047217:mannose 1-phosphate; D019298:Glucose-6-Phosphate; D002238:Carbohydrate Epimerases; D008359:Mannose-6-Phosphate Isomerase; D005956:Glucose-6-Phosphate Isomerase