Preparation of a homogeneous and stable form of bovine milk lipoprotein lipase.
Lipoprotein lipase was purified to homogeneity from bovine skim milk by a two-step procedure using chromatography on heparin-Sepharose. As determined by gradient-polyacrylamide gel electrophoresis in sodium dodecyl sulfate, purified lipoprotein lipase showed a single band with an apparent molecular weight of 55,000. The use of Triton N-101 in the washing buffers was the major improvement from previously reported purification procedures that resulted in a stable homogeneous preparation of the enzyme.
D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D005260:Female; D006493:Heparin; D008071:Lipoprotein Lipase / Q000302:isolation & purification*; D008892:Milk / Q000201:enzymology*; D008970:Molecular Weight; D011092:Polyethylene Glycols
D011092:Polyethylene Glycols; D006493:Heparin; D008071:Lipoprotein Lipase