Plastid enzymes of terpenoid biosynthesis. Purification and characterization of gamma-tocopherol methyltransferase from Capsicum chromoplasts.
gamma-Tocopherol methyltransferase was solubilized and purified from Capsicum chromoplast membranes by a combination of standard fractionation techniques. The purified enzyme was electrophoretically homogeneous, and its molecular weight, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, was 33,000. In the absence of detergent, the enzyme formed high molecular weight aggregates. Several properties of the enzyme have been determined. The Km values were 2.5 and 13.7 microM for S-adenosylmethionine and gamma-tocopherol, respectively. The enzyme was able to transfer the methyl group S-adenosylmethionine to N-4-azido-2-nitrophenyl-beta-alanyl-gamma-tocopherol. The rate of transfer was less efficient compared to gamma-tocopherol. In the presence of ultraviolet light, this analog inhibited the gamma-tocopherol methyltransferase activity.
D001386:Azides; D005638:Fruit / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008780:Methyltransferases / Q000302:isolation & purification* / Q000378:metabolism; D008970:Molecular Weight; D011136:Polysorbates; D012436:S-Adenosylmethionine / Q000378:metabolism; D012995:Solubility; D013997:Time Factors; D014810:Vitamin E / Q000031:analogs & derivatives / Q000378:metabolism; D024504:gamma-Tocopherol / Q000031:analogs & derivatives*
D001386:Azides; D011136:Polysorbates; D014810:Vitamin E; D012436:S-Adenosylmethionine; D024504:gamma-Tocopherol; C047260:N-4-azido-2-nitrophenyl-beta-alanyl-gamma-tocopherol; D008780:Methyltransferases; C047259:gamma-tocopherol methyltransferase