title: RISH V. Application to monoclonal antibody production.
abstract: RISH considers that cell surface components involved in like cell identification are not involved in the structure of the plasma membrane per se and are attached to a part of their mRNA. The mRNA then acts as a template for the synthesis of DNA. Thus the component at the cell surface is attached to an RNA/DNA receptor. If there is a conformational change in the component (antigen) this will cause a distortion in its RNA/DNA receptor. This distortion is then detected by a tissue specific T lymphocyte which removes all or part of the RNA/DNA receptor from the aberrant cell and the lymphocyte then undergoes replication. During this process receptor RNA/DNA is incorporated into the daughter lymphocyte which becomes a B lymphocyte/plasma cell producing immunoglobulin. The initial tissue specific T lymphocyte becomes a dual functional helper/suppressor cell. The plasma cell after the initial immune response becomes a circulating memory B cell displaying IgM or IgD. If this cell complexes an antigen with its surface IgM or IgD a humoral immune response will be developed as previously described, but in this case the antibodies produced will be anti-idiotypic antibodies. The anti-idiotypic antibodies will regulate the production of the antibody directed against the antigen per se. The anti-idiotypic antibodies will in turn be regulated by a second anti-idiotypic antibody. In RISH five such anti-idiotypic systems may be involved in regulating the immune response to the initial non-immunoglobulin antigen. Based on the RISH anti-idiotypic mechanism a system is briefly described whereby human memory B cells, to a particular antigen, may be isolated. These B cells may then be activated to secrete immunoglobulin with autologous isolated anti-idiotypic antibodies. These activated cells may then be infected with E.B. virus to establish an immortal cell line of B cells secreting the immunoglobulin of interest.
mesh_terms: D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal / Q000276:immunology; D000917:Antibody Formation; D002454:Cell Differentiation; D006706:Homeostasis; D006801:Humans; D007109:Immunity; D007111:Immunity, Cellular; D007126:Immunoglobulin Allotypes / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D007156:Immunologic Memory; D007157:Immunologic Surveillance; D008214:Lymphocytes / Q000502:physiology
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D007126:Immunoglobulin Allotypes; D007130:Immunoglobulin Idiotypes