Decreased utilization of [2-3H]glycerol in phospholipid and neutral glyceride biosynthesis in the retina of streptozotocin-diabetic rats.
The effect of streptozotocin (STZ)-induced diabetes on lipid uptake was studied in the rat retina. The intravitreal incorporation of [2-3H]glycerol was followed in retinal lipids from the first day of diabetes (acute state) up to 20 wk (chronic state). Total lipid incorporation decreased 50% 2 d after injection of STZ; the labeling remained lower than the control values throughout the 20 wk period studied. In the chronic state of diabetes, the time-course of the incorporation of [2-3H]glycerol during the first 2 h after its injection displayed a pattern of incorporation similar to that of the controls. The possibility that the decreased utilization of [2-3H]glycerol in the lipid pathway results from a competition between the injected [3H]glycerol and the glycolytic pathway during diabetes in rat retina in vivo is discussed.
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D003930:Diabetic Retinopathy / Q000378:metabolism; D005989:Glycerides / Q000096:biosynthesis*; D005990:Glycerol / Q000378:metabolism*; D008297:Male; D010712:Phosphatidic Acids / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D012160:Retina / Q000378:metabolism*
D001786:Blood Glucose; D005989:Glycerides; D010712:Phosphatidic Acids; D005990:Glycerol