Development of the heterogeneous distribution of carbamoyl-phosphate synthetase (ammonia) in rat-liver parenchyma during postnatal development.
Carbamoyl-phosphate synthetase (ammonia) is homogeneously distributed in rat-liver parenchyma at birth, as demonstrated by immunohistochemistry. A heterogeneous distribution can first be demonstrated at 6 days post partum, but can be masked by use of a too sensitive detection system. This heterogeneity is established by a decrease in enzyme content around the hepatic venules and a considerable increase in enzyme content in the remaining parenchyma. The perivenous decrease in enzyme content does not occur in all hepatocytes synchronously. The adult type of heterogeneity is characterized by a perivenous layer, only two to three cells thick, in which carbamoyl-phosphate synthetase can no longer be detected, irrespective of the sensitivity of the assay used. This situation is fully established at the age of two months.
D000375:Aging; D000818:Animals; D002222:Carbamoyl-Phosphate Synthase (Ammonia) / Q000378:metabolism*; D007118:Immunoassay; D007527:Isoenzymes / Q000378:metabolism; D008025:Ligases / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000201:enzymology / Q000254:growth & development*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D007527:Isoenzymes; D008025:Ligases; D002222:Carbamoyl-Phosphate Synthase (Ammonia)