title: Oxidation of protoporphyrinogen in the obligate anaerobe Desulfovibrio gigas.
abstract: The anaerobic oxidation of protoporphyrinogen to protoporphyrin was demonstrated in extracts of Desulfovibrio gigas. Protoporphyrin formation occurred in the presence of nitrite, hydroxylamine, sulfite, thiosulfate, ATP plus sulfate, NAD+, NADP+, flavin adenine dinucleotide, flavin mononucleotide, fumarate, 2,6-dichlorophenol-indophenol, methyl viologen, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. With dialyzed cell extracts, highest activities were observed with sulfite, NAD+, and NADP+ as electron acceptors. The enzyme for protoporphyrinogen oxidation was localized in the membrane of D. gigas and displayed optimal activity at pH 7.3 and 28 degrees C.
mesh_terms: D003901:Desulfovibrio / Q000378:metabolism*; D004579:Electron Transport; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000032:analysis; D044925:Oxidoreductases Acting on CH-CH Group Donors; D011166:Porphyrins / Q000378:metabolism*; D050768:Protoporphyrinogen Oxidase; D011524:Protoporphyrins / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D011166:Porphyrins; D011524:Protoporphyrins; C011539:protoporphyrinogen; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; D050768:Protoporphyrinogen Oxidase