title: Oestrone sulphate, adipose tissue, and breast cancer.
abstract: Oestrone sulphate, the oestrogen in highest concentration in the plasma, may play a role in the induction and growth of breast cancers. By enzymolysis and radioimmunoassay, oestrone sulphate concentrations were measured in 3 biological fluids. High concentrations of the conjugate (up to 775 nmol/l) were detected in breast cyst fluids from some premenopausal women, the concentrations in blood plasma (0.91-4.45 nmol/l) being much lower. Concentrations in the plasmas from postmenopausal women with (0.23-4.63 nmol/l) or without (0.18-1.27 nmol/l) breast cancer were still lower. Oestrone sulphate concentration in cow's milk or cream (0.49-0.67 nmol/l) was also low: dietary intake in these fluids is probably of little consequence. The capacity of breast tissues for hydrolysis of oestrone sulphate was examined in two ways: In tissue slices incubated with 85 pM (3H) oestrone sulphate solution at 37 degrees C, cancers (131-412 fmol/g tissue/hr) and adipose tissues (23-132 fmol/g tissue/hr) hydrolysed significantly more sulphate than did benign tissues (1-36 fmol/g tissue/hr). In tissue homogenates incubated with 5-25 microM [3H] oestrone sulphate at 37 degrees much higher capacities for hydrolysis (nmol/g tissue/hr) were demonstrated with a Km of 2-16.5 microM: cancers (34-394) and benign tissues (9-485) had significantly higher sulphatase activities than adipose tissues (9-39). On a protein basis, however, the sulphatase activities in the 3 tissues were comparable. It is concluded that oestrone sulphate is present in breast cysts and blood plasma and that in vitro, the conjugated hormone can be hydrolysed by breast tissues. The biological significance of these findings in vivo remains to be established.
mesh_terms: D000273:Adipose Tissue / Q000032:analysis*; D000818:Animals; D001940:Breast / Q000032:analysis; D001943:Breast Neoplasms / Q000032:analysis* / Q000473:pathology; D002417:Cattle; D004970:Estrone / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood; D005260:Female; D005348:Fibrocystic Breast Disease / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D008593:Menopause; D008892:Milk / Q000032:analysis; D011863:Radioimmunoassay
chemical_list: D004970:Estrone; C017296:estrone sulfate