Use of bonded-phase silica sorbents for rapid sampling of impurities in illicit amphetamine for high-performance liquid chromatographic analyses.
A simple and rapid method has been developed for the extraction of impurities from illicit amphetamine samples using bonded-phase silica sorbents. The drug is dissolved in phosphate buffer (pH 7) and added to a C8 Bond Elut extraction column. The column is washed with water, and the impurities are then eluted with acetonitrile. The eluate is directly injected into the liquid chromatograph. This sample preparation technique has been compared with the traditional liquid-liquid extraction method. High-performance liquid chromatographic analysis of the impurities is carried out on a reversed-phase C18 column with an acetonitrile-water gradient as mobile phase. Peaks are monitored by UV detection at 220 and 254 nm. A series of seized amphetamine samples has been analysed, and the procedure gives detailed impurity patterns suitable for the comparison of samples. Compounds are identified by absorbance ratios (A220/A254).
D000661:Amphetamine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004340:Drug Contamination; D008401:Gas Chromatography-Mass Spectrometry; D013287:Illicit Drugs / Q000032:analysis*; D004364:Pharmaceutical Preparations / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D013287:Illicit Drugs; D004364:Pharmaceutical Preparations; D000661:Amphetamine