Potassium-induced reverse transformation of cells infected with a temperature-sensitive transformation mutant virus.
High potassium concentrations altered the morphology and the ability to grow in soft agar in 6m2 cells, a clone of rat kidney cells infected with a temperature-sensitive mutant of Moloney sarcoma virus. Approximately 60% of cells exhibited normal morphology in the presence of 94.8 mM potassium in isotonic medium at the temperature permissive for transformation, whereas 100% were normal at 72 mM potassium in hypertonic media. A significant reduction of growth in soft agar was also induced with these conditions. However, the synthesis ratio of virus-specified transforming protein to marker viral protein was not altered. Na+K+-ATPase might play a role in this reverse-transformation process.
D000818:Animals; D002454:Cell Differentiation; D002472:Cell Transformation, Viral / Q000187:drug effects*; D002478:Cells, Cultured; D003470:Culture Media; D006982:Hypertonic Solutions; D007668:Kidney / Q000473:pathology*; D008980:Moloney murine sarcoma virus / Q000235:genetics / Q000502:physiology; D009154:Mutation; D009994:Osmolar Concentration; D010641:Phenotype; D011188:Potassium / Q000494:pharmacology*; D051381:Rats; D012513:Sarcoma, Experimental / Q000235:genetics* / Q000473:pathology; D013696:Temperature
D003470:Culture Media; D006982:Hypertonic Solutions; D011188:Potassium