An improved preservation technique for cells of hemopoietic origin.
A new technique for the cryopreservation of cell lines of hemopoietic origin is described. It uses a combination of 10% polyvinylpyrrolidone or hydroxyethylstarch with 15% glycerol, a slow stepwise addition of the cryoprotectants before freezing, cooling at 4 degrees C/min, rapid rewarming, and a slow step-wise dilution after thawing. This technique has given improved recovery rates with a number of basophil/mast cell lines, as well as a wide range of hemopoietic cells including hybridomas.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D005615:Freezing; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D051379:Mice; D051381:Rats; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods