title: Two biochemically distinct populations of histaminocytes separated by isokinetic sedimentation of dispersed rat gastric cells.
abstract: Two populations of histaminocytes, with different sedimentation rates (SR), were separated by a computer developed isokinetic gradient using dispersed rat gastric mucosal cells. Histamine content, histidine decarboxylase (HDC) activity and incorporation of radiolabelled histidine metabolites were used to assess the migration of specific cells throughout the gradients. One histaminocyte population, with cells of lower SR, contained high HDC activity and undetectable levels of histamine, whereas the other population, with cells of higher SR, contained lower HDC activity and high concentration of histamine. Both types of histaminocytes incorporated 3H-histidine metabolites. Electron microscopy showed that the fractions containing histaminocytes with lower SR had 3.5 times more endocrine ECL cells than the original population of dispersed fundic cells and lacked A and D cells, whereas the fractions with histaminocytes of higher SR were associated with a 2.7 times higher concentration of A and D cells and with a 7.7 times higher ratio of a variety of partial cells with a distinct mitochondrial morphology. These results are consistent with prior novel information regarding the separation of two populations of rat histaminocytes using different sedimentation techniques.
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient; D004702:Endocrine Glands / Q000166:cytology; D005748:Gastric Fundus; D005753:Gastric Mucosa / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D006632:Histamine / Q000378:metabolism*; D006640:Histidine Decarboxylase / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D008928:Mitochondria / Q000648:ultrastructure; D010295:Parietal Cells, Gastric / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium
chemical_list: D014316:Tritium; D006632:Histamine; D006640:Histidine Decarboxylase