title: An automated liquid-chromatographic system for convenient determination of glycated hemoglobin A1c.
abstract: For quantification of glycated hemoglobin (HbA1c), we compared an automated system (Pharmacia Fast Protein Liquid Chromatography), which separates and determines HbA1c, with a commercial disposable-minicolumn kit (Boehringer-Mannheim), which separates total HbA1. We studied 41 diabetic women and 79 apparently normal women on their first postparturition day. The automated method was more precise (within-run CV 0.98-4.16%) than the kit method (within-run CV 3.67-7.77%). Results by the two methods correlated well for both control (p less than 0.001) and diabetic (p less than 0.05) groups. Values for HbA1c correlated significantly with fasting blood-glucose concentrations in controls and diabetics (intraclass correlation coefficient rI = 0.822 and 0.851, respectively, p less than 0.001) as well as with 1- and 2-h values for glucose after a 75-g glucose load in the control group (rI = 0.649 and 0.846, respectively, p less than 0.001). For HbA1 no such significant correlation was found except with values for fasting blood glucose in diabetics (rI = 0.745, p less than 0.001). Evaluation of HbA1c was a more sensitive index of glycemic status. The automated system is convenient, reliable, and easily operated.
mesh_terms: D000328:Adult; D001322:Autoanalysis; D001786:Blood Glucose / Q000032:analysis; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid / Q000379:methods; D003920:Diabetes Mellitus / Q000097:blood; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans; D011933:Reagent Kits, Diagnostic
chemical_list: D001786:Blood Glucose; D006442:Glycated Hemoglobin; D011933:Reagent Kits, Diagnostic