Regulation of 3-ketosteroid-1-en-dehydrogenase activity of Arthrobacter globiformis cells by a respiratory chain.
It has been shown that 3-ketosteroid-1-en-dehydrogenase localized in a cytoplasmic membrane donates reducing equivalents to a respiratory chain directly which passes them over to oxygen. Microbial hydrocortisone oxidation is coupled with energy generation in the form of the H+ transmembrane potential. Electron transfer via a respiratory chain is the limiting stage in the process of hydrocortisone 1-en-dehydrogenation.
D001173:Arthrobacter / Q000201:enzymology*; D003486:Cyanides / Q000494:pharmacology; D003593:Cytoplasm / Q000201:enzymology; D004579:Electron Transport / Q000187:drug effects; D006854:Hydrocortisone / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D010088:Oxidoreductases / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects
D003486:Cyanides; D010088:Oxidoreductases; C022656:3-oxosteroid delta(1) dehydrogenase; D006854:Hydrocortisone