Antibodies to Z DNA stabilized with polyarginine.
The left-handed form of poly(dG-m5dC).poly(dG-m5dC) induced by heating the copolymer in the presence of magnesium and stabilized with polyarginine can be used to raise antibodies in rabbits. These antibodies are able to recognize the Z conformation of both methylated and nonmethylated forms of the copolymers. In the same experimental conditions, hypermethylated B DNA is not recognized by these antibodies.
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002942:Circular Dichroism; D004247:DNA / Q000276:immunology*; D005374:Filtration; D008745:Methylation; D009690:Nucleic Acid Conformation; D010455:Peptides / Q000494:pharmacology*; D011817:Rabbits; D013696:Temperature
D000906:Antibodies; D010455:Peptides; C015462:polyarginine; D004247:DNA