title: Meiosis-inducing substances in human preovulatory follicular fluid related to time of follicle aspiration and to the potential of the oocyte to fertilize and cleave in vitro.
abstract: Meiosis-inducing substance (MIS) and steroid and gonadotropic hormones were investigated in 41 preovulatory follicular fluids (FFs) aspirated at either 0, 12, or 36 hours after human chorionic gonadotropin (hCG) administration in 25 women with clomiphene citrate-stimulated cycles. Twenty-one oocytes were recovered from these FFs and subjected to in vitro fertilization. MIS activity was present in 25 (61%) of the FFs. The frequency of MIS-active FFs increased from 11% (1 of 9) at 0 hours and 40% (2 of 5) at 12 hours to 81% (22 of 27) at 36 hours after hCG administration (P less than 0.001). The concentration of hormones in MIS-active FFs was not significantly different from that of MIS-inactive FFs. Twelve (86%) of 14 oocytes that fertilized and cleaved in vitro were recovered from MIS-active FFs. By contrast, all seven oocytes that remained unfertilized in vitro were recovered from MIS-inactive FFs. These findings support the notion that resumption of meiosis in the preovulatory oocyte is triggered by MIS in FF and suggest that follicular MIS production may be one of the factors that determines the success of in vitro fertilization and early embryonic development.
mesh_terms: D000735:Androstenedione / Q000097:blood; D006063:Chorionic Gonadotropin / Q000627:therapeutic use; D002996:Clomiphene / Q000627:therapeutic use; D004958:Estradiol / Q000097:blood; D005122:Exudates and Transudates / Q000032:analysis*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008540:Meiosis; D009865:Oocytes / Q000166:cytology*; D006080:Ovarian Follicle / Q000378:metabolism*; D010062:Ovulation Induction; D011374:Progesterone / Q000097:blood; D013396:Suction; D013997:Time Factors
chemical_list: D006063:Chorionic Gonadotropin; D002996:Clomiphene; D000735:Androstenedione; D011374:Progesterone; D004958:Estradiol