Active transendothelial transport of albumin. Interstitium to lumen.
Cultured porcine pulmonary artery endothelial monolayers actively transport albumin from interstitium to lumen. The active process favors interstitial to luminal transport by a factor of 10 even in the face of a 2:1 luminal:interstitial albumin gradient. The active process is abolished by treatment with 1 mM sodium cyanide. Dextran is not actively transported. This process could be important in determining transvascular fluid balance and transfer of macromolecules across the endothelium.
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002478:Cells, Cultured; D004727:Endothelium / Q000378:metabolism*; D007700:Kinetics; D012709:Serum Albumin / Q000378:metabolism*; D012966:Sodium Cyanide / Q000494:pharmacology; D013552:Swine
D012709:Serum Albumin; D012966:Sodium Cyanide