title: The effects of soman, in vivo and in vitro, on aldolase activity.
abstract: We examined the in vivo and in vitro effects of soman on aldolase activity. Male rats were killed at 4, 6, 9 and 12.5 min after subcutaneous (s.c.) administration of 90 micrograms/kg soman. At 4 min after treatment, aldolase activity was inhibited 55-90% compared to control in cerebral cortex, brainstem, mid-brain and cerebellum; and up to 50% of control in diaphragm and muscle. By 12.5 min, aldolase activity in all areas had returned to control levels except in the diaphragm, which still exhibited 36% inhibition. In vitro activity of rabbit muscle aldolase was not inhibited by 10(-3) M soman. In contrast, diisopropylfluorophosphonate (DFP, 10(-3)-10(-2) M) acted as a competitive inhibitor of aldolase activity in vitro. The results suggest that in vivo, soman effects on aldolase activity are transitory. Any long-lasting effects of soman on aldolase activity may occur only in the periphery, and not in the central nervous system.
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D005634:Fructose-Bisphosphate Aldolase / Q000037:antagonists & inhibitors*; D066298:In Vitro Techniques; D007531:Isoflurophate / Q000633:toxicity; D008297:Male; D009132:Muscles / Q000201:enzymology; D011817:Rabbits; D051381:Rats; D012999:Soman / Q000633:toxicity*
chemical_list: D007531:Isoflurophate; D012999:Soman; D005634:Fructose-Bisphosphate Aldolase