Characterization of strain specific typing antisera for genetic monitoring of inbred strains of rats.
Strain-specific typing antisera (SSTA) were prepared for six inbred strains of rats by using a pooled immunization protocol. The SSTA were used in both a haemagglutination assay and a complement dependent microcytotoxicity assay to compare the usefulness of the two test systems. Both assays were simple, reliable and repeatable, and each system had distinct advantages and disadvantages. The haemagglutination assay was faster and required less specialized equipment than the microcytotoxicity assay. On the other hand, interpretation of results in the microcytotoxicity assay was easier and more objective. It was concluded that SSTA could be used with the microcytotoxicity assay and/or the haemagglutination assay to provide a simple and effective genetic monitoring method for inbred strain of rats.
D000818:Animals; D003601:Cytotoxicity Tests, Immunologic; D006386:Hemagglutination Tests; D007106:Immune Sera / Q000276:immunology*; D007114:Immunization; D008214:Lymphocytes / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains / Q000235:genetics*
D007106:Immune Sera