Amperometric detection of reducing carbohydrates in high-performance liquid chromatography using an amino-bonded column and acetonitrile-water as the eluent.
Amperometric detection of reducing carbohydrates using copper bis(phenanthroline) as the mediator in the post-column reaction was applied to the partition mode of high-performance liquid chromatography using an amino-bonded column with acetonitrile-water as the eluent. The proposed method appears to be the most sensitive means of detection in the partition separation of carbohydrates. Glucose can be determined at levels down to 5 pmol (1 ng injection). Excellent selectivity was demonstrated in examples of chromatograms obtained with fruit juices and human urine.
D000097:Acetonitriles; D002241:Carbohydrates / Q000032:analysis* / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D004040:Dietary Carbohydrates / Q000032:analysis; D004563:Electrochemistry; D005638:Fruit / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D013696:Temperature; D014867:Water
D000097:Acetonitriles; D002241:Carbohydrates; D004040:Dietary Carbohydrates; D014867:Water; C032159:acetonitrile