Stereoscopic images in confocal (tandem scanning) microscopy.
Stereoscopic pair images with parallel projection geometry are obtained by through-focusing along two inclined axes while recording two (summed and stacked) images with a microscope with a very shallow depth of field. The two stack images sample the same depth slice of translucent or reflective specimens. The method will work most conveniently with a tandem scanning microscope (a direct-view, confocal scanning optical microscope). This is a direct method for recording stereo images that can be used to the limit of resolution in optical microscopy. It demonstrates a previously unrealized advantage of confocal optical microscopy.
D000818:Animals; D001842:Bone and Bones / Q000033:anatomy & histology; D002531:Cerebellum / Q000033:anatomy & histology; D051379:Mice; D008853:Microscopy / Q000379:methods*; D051381:Rats; D013116:Spinal Cord / Q000033:anatomy & histology