Magnitude of ouabain-sensitive respiration in the liver of growing, lactating and starved sheep.
Oxygen consumption and ouabain-sensitive respiration was measured for liver biopsies from lactating and non-lactating ewes and for hepatocytes isolated from mature, dry ewes. O2 consumption, ouabain-sensitive respiration and 86Rb+ uptake were also measured for hepatocytes isolated from lambs, fed adult sheep and adult sheep starved for 5 d. Ouabain-sensitive respiration in the liver of ewes at peak lactation accounted for 45% of the total liver O2 consumption. This percentage was 24-37% higher (P less than 0.05) than measurements made during late lactation and during the non-lactating period. Total O2 consumption and ouabain-sensitive respiration rates of lamb hepatocytes were greater (P less than 0.05) than similar measurements for hepatocytes isolated from adult sheep. Ouabain-sensitive 86Rb+ uptake by hepatocytes from fed sheep was up to six times greater (P less than 0.05) than that by cells from starved sheep. The magnitude of ouabain-sensitive respiration of hepatocytes from starved sheep was 62% lower (P less than 0.05) than that for hepatocytes from fed sheep.
D000818:Animals; D005260:Female; D007774:Lactation; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology*; D010101:Oxygen Consumption / Q000187:drug effects*; D011247:Pregnancy; D011868:Radioisotopes; D012413:Rubidium / Q000378:metabolism; D012756:Sheep; D013217:Starvation / Q000378:metabolism*
D011868:Radioisotopes; D010042:Ouabain; D012413:Rubidium