Antibodies to the alpha-subunit of insulin receptor from eggs of immunized hens.
Simple methods for the generation, purification, and assay of antibodies to the alpha-subunit of insulin receptor from eggs of immunized hens have been described. Chicken antibodies against the alpha-subunit inhibit insulin binding to the receptor and stimulate glucose oxidation as well as autophosphorylation of the beta-subunit. Thus the properties of chicken antibodies are very similar to those of antibodies found in human autoimmune diseases and different from rabbit antibodies obtained against the same antigen.
D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D000918:Antibody Specificity; D002645:Chickens; D005260:Female; D005947:Glucose / Q000378:metabolism; D007114:Immunization; D007136:Immunoglobulins / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification; D007330:Insulin Antibodies / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification; D008099:Liver / Q000378:metabolism; D010063:Ovum / Q000276:immunology*; D010766:Phosphorylation; D051381:Rats; D011919:Rats, Inbred Strains; D011972:Receptor, Insulin / Q000008:administration & dosage / Q000276:immunology* / Q000378:metabolism
C016512:IgY; D007136:Immunoglobulins; D007330:Insulin Antibodies; D011972:Receptor, Insulin; D005947:Glucose