title: Studies by electron microscopy on the assembly of duck hepatitis B virus in the liver.
abstract: Liver specimens from 1-day-old ducklings infected in ovo with maternally transmitted duck hepatitis B virus (DHBV) were examined by electron microscopy. Complete and incomplete DHBV particles were located within hypertrophied cisternae of the endoplasmic reticulum of the hepatocytes. The complete viral particles found intracellularly have inner cores with a diameter ranging from 35 to 37.5 nm and an outer coat or envelope. The whole particle measures approximately 45-65 nm in diameter. Naked core particles were located in the nuclei, free in the cytoplasm, and also near or on the cisternal membrane of the endoplasmic reticulum on the cytoplasmic face. Duck hepatitis B virions appear to share morphological characteristics with the viral coat and core of human hepatitis B virus (HBV). Electron microscopy suggested that the core particles of DHBV migrate from the nucleus into the cytoplasm through the nuclear pores. The complete viral particles are probably formed by protrusion of the core particles through the endoplasmic reticulum and by simultaneous encapsulation with a coat derived from the endoplasmic reticulum.
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000382:microbiology; D003593:Cytoplasm / Q000382:microbiology; D004372:Ducks / Q000382:microbiology; D004721:Endoplasmic Reticulum / Q000382:microbiology; D006515:Hepatitis B virus / Q000254:growth & development* / Q000648:ultrastructure; D008099:Liver / Q000382:microbiology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D014771:Virion / Q000254:growth & development; D014779:Virus Replication