Localization of the human salivary protein complex (SPC) to chromosome band 12p13.2.
In situ hybridization of a 3H-labeled probe containing a fragment from PRP-1, a genomic clone with human salivary proline-rich protein gene sequences, revealed significant labeling on the short arm of human chromosome 12 in metaphase preparations from two individuals. Fifty-three percent of metaphases exhibited labeling on one or both chromosomes 12. Additional cells scored at the 850-1,000 band level revealed a significant proportion (52% [32/61] grains, p less than 0.005) of the labeled sites on chromosome 12 to be on band 12p13.2. This probe for a human salivary proline-rich protein gene fragment, probably PMS, is from a cluster of 13 linked genes designated as the human salivary protein complex (SPC). Studies of the DNA of human-mouse somatic-cell hybrids have assigned the SPC to chromosome 12, but have not provided a regional localization (Azen et al, 1985). This paper reports the localization of the SPC to a specific chromosomal band, 12p13.2.
D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002906:Chromosomes, Human, 6-12 and X; D004247:DNA / Q000235:genetics; D005260:Female; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells; D007621:Karyotyping; D008297:Male; D051379:Mice; D009693:Nucleic Acid Hybridization; D010455:Peptides / Q000235:genetics*; D055232:Proline-Rich Protein Domains; D012471:Salivary Proteins and Peptides / Q000235:genetics*
D010455:Peptides; D012471:Salivary Proteins and Peptides; D004247:DNA