The EM autoradiographic distribution of label in tissue blocks of pallidum incorporating [3H]-Leu-enkephalin and [3H]-naloxone in vitro.
The distribution of radioactivity in pallidal tissue blocks incubated in the presence of either a tritiated enkephalin analogue or [3H]-naloxone, was examined using EM autoradiography. The quantitative evaluation was based on the principle of proportional grain counting. The results showed a preferential accumulating of silver grains over dendrites in the enkephalin-labelled tissue. Following naloxone-labelling, a slight enrichment of grains over axonal elements was found. The findings provide ultrastructural support for previous biochemical data which suggested that different types of opiate binding sites have different tissue localizations.
D000818:Animals; D001345:Autoradiography; D001369:Axons / Q000648:ultrastructure; D003712:Dendrites / Q000648:ultrastructure; D004743:Enkephalin, Leucine / Q000378:metabolism*; D005917:Globus Pallidus / Q000033:anatomy & histology*; D008854:Microscopy, Electron; D009270:Naloxone / Q000378:metabolism*; D009474:Neurons / Q000648:ultrastructure; D051381:Rats; D013569:Synapses / Q000648:ultrastructure
D009270:Naloxone; D004743:Enkephalin, Leucine