RISH V. Application to monoclonal antibody production.
RISH considers that cell surface components involved in like cell identification are not involved in the structure of the plasma membrane per se and are attached to a part of their mRNA. The mRNA then acts as a template for the synthesis of DNA. Thus the component at the cell surface is attached to an RNA/DNA receptor. If there is a conformational change in the component (antigen) this will cause a distortion in its RNA/DNA receptor. This distortion is then detected by a tissue specific T lymphocyte which removes all or part of the RNA/DNA receptor from the aberrant cell and the lymphocyte then undergoes replication. During this process receptor RNA/DNA is incorporated into the daughter lymphocyte which becomes a B lymphocyte/plasma cell producing immunoglobulin. The initial tissue specific T lymphocyte becomes a dual functional helper/suppressor cell. The plasma cell after the initial immune response becomes a circulating memory B cell displaying IgM or IgD. If this cell complexes an antigen with its surface IgM or IgD a humoral immune response will be developed as previously described, but in this case the antibodies produced will be anti-idiotypic antibodies. The anti-idiotypic antibodies will regulate the production of the antibody directed against the antigen per se. The anti-idiotypic antibodies will in turn be regulated by a second anti-idiotypic antibody. In RISH five such anti-idiotypic systems may be involved in regulating the immune response to the initial non-immunoglobulin antigen. Based on the RISH anti-idiotypic mechanism a system is briefly described whereby human memory B cells, to a particular antigen, may be isolated. These B cells may then be activated to secrete immunoglobulin with autologous isolated anti-idiotypic antibodies. These activated cells may then be infected with E.B. virus to establish an immortal cell line of B cells secreting the immunoglobulin of interest.
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal / Q000276:immunology; D000917:Antibody Formation; D002454:Cell Differentiation; D006706:Homeostasis; D006801:Humans; D007109:Immunity; D007111:Immunity, Cellular; D007126:Immunoglobulin Allotypes / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D007156:Immunologic Memory; D007157:Immunologic Surveillance; D008214:Lymphocytes / Q000502:physiology
D000906:Antibodies; D000911:Antibodies, Monoclonal; D007126:Immunoglobulin Allotypes; D007130:Immunoglobulin Idiotypes