Evidence that the 32, 38 and 20 kilodalton surface antigens of schistosomula and schistosomes are a family of conserved proteins.
The structures of the 38, 32 and 20 kDa surface antigens isolated from schistosomula and adult worms of Schistosoma mansoni were compared by two-dimensional peptide mapping, by immunological analysis and by one- and two-dimensional electrophoresis. Peptide mapping showed a high degree of similarity between the isolated antigens from both parasite stages. The NIMP/M47 monoclonal antibody showed cross-reactivity between the 32 and the 20 kDa antigens under denaturating and non-denaturating conditions, as demonstrated by immunoprecipitation and Western blotting. It is concluded that these antigens constitute a homologous family of surface antigens.
D000818:Animals; D000947:Antigens, Helminth / Q000302:isolation & purification; D000954:Antigens, Surface / Q000032:analysis; D011232:Chemical Precipitation; D007526:Isoelectric Point; D008565:Membrane Proteins / Q000276:immunology; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D012550:Schistosoma mansoni / Q000254:growth & development / Q000276:immunology*
D000947:Antigens, Helminth; D000954:Antigens, Surface; D008565:Membrane Proteins; D010446:Peptide Fragments