Chilling followed by incubation at 37 degrees causes a flow of 3H-choline labeled phospholipids from nuclear-associated endoplasmic reticulum to other endomembranes of L-cells.
The exposure of L-cells to a period of 15 min incubation in ice followed by a return to normal culture conditions caused distinct alterations in the distribution pattern of 3H-choline incorporation in phospholipids in subcellular fractions. The amount of radioactivity appearing in the nuclei and nuclear-associated endoplasmic reticulum decreased while that in the mitochondrial and endoplasmic reticulum membrane fractions increased, suggesting a precursor-product relationship. These changes appeared to occur in a linear fashion. Furthermore, the increase in radioactivity in individual endoplasmic reticulum subfractions differed in that label increased fivefold in light rough membranes but only about twofold in the HR and S subfractions.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002794:Choline / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism*; D007700:Kinetics; D007739:L Cells / Q000378:metabolism; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism*; D013696:Temperature; D014316:Tritium
D010713:Phosphatidylcholines; D014316:Tritium; D002794:Choline