Determination of alpha-amanitin and beta-amanitin in human biological fluids by high-performance liquid chromatography.
A high-performance liquid chromatographic assay of alpha-amanitin and beta-amanitin in human serum, urine, or stomach washings is described. Sample preparation involves a chemical step with deproteinization and organic solvent treatment, and a selective cleanup and concentration step on reversed-phase prepacked cartridges. Separations are performed on a reversed-phase analytical column under isocratic conditions with uv detection at 280 nm. The method allows the quantitation of alpha- and beta-amanitin separately with a detection limit of 10 ng/ml for both toxins.
D000546:Amanitins / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D005612:Freeze Drying; D005766:Gastrointestinal Contents / Q000032:analysis; D006801:Humans; D008832:Microchemistry; D012997:Solvents
D000546:Amanitins; D012997:Solvents; C049197:beta-amanitin