A sensitive chromogenic Limulus amoebocyte lysate micro-assay for detection of endotoxin in human plasma and in water.
This chromogenic endotoxin assay involves a 45 min incubation of plasma extract or water with a mixture of Limulus amoebocyte lysate and the chromogenic substrate S-2423. Absorbance is measured in micro-titre plates. The assay allows the detection of 0.2 pg endotoxin per ml in water (0.002 Endotoxin Units/ml) and 1 pg endotoxin per ml in plasma (0.012 Endotoxin Units/ml). The intra-assay coefficient of variation is less than 5%.
D000814:Aniline Compounds / Q000378:metabolism; D002863:Chromogenic Compounds; D004731:Endotoxins / Q000032:analysis* / Q000097:blood; D005260:Female; D006801:Humans; D007082:Ileum / Q000601:surgery; D008033:Limulus Test / Q000379:methods*; D008875:Middle Aged; D011183:Postoperative Complications; D012772:Shock, Septic / Q000097:blood / Q000209:etiology; D014867:Water / Q000032:analysis*
D000814:Aniline Compounds; D002863:Chromogenic Compounds; D004731:Endotoxins; D014867:Water; C019498:4-nitroaniline