Protein synthesis in cells infected by chilo iridescent virus (iridovirus, type 6).
Synthesis of the polypeptides induced in CIV-infected cells was studied using radiolabelled methionine in a permissive cell line of Choristoneura fumiferana. Analysis of labelled cell extracts by high resolution polyacrylamide gel electrophoresis (PAGE) revealed the sequential appearance of at least 28 structural and non-structural polypeptides in permissive conditions. This was confirmed by analysis of protein synthesis in non-permissive cell lines of Lymantria dispar, Aedes albopictus or by the use of protein, RNA and DNA synthesis inhibitors in the permissive cell line.
D000818:Animals; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D004261:DNA Replication; D003609:Dactinomycin / Q000494:pharmacology; D007313:Insecta; D007497:Iridoviridae / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis*; D015678:Viral Structural Proteins; D014779:Virus Replication
D014764:Viral Proteins; D015678:Viral Structural Proteins; D003609:Dactinomycin; D003513:Cycloheximide