Large-scale production of monoclonal antibodies in dialysis tubing.
A simple procedure for the large-scale production of monoclonal antibodies has been developed. Hybridomas were grown in dialysis tubing containing medium with a low concentration of proteins. The dialysis tubing was inserted into a flask with medium containing 10% or 30% foetal calf serum. The flask was placed on a roller and medium was changed every other day. Monoclonal antibodies were harvested after about 10 days in culture. Immunoglobulin concentrations up to 5.4 mg/ml and cell yields of 85 X 10(6) cells/ml have been obtained. The low concentration of contaminating low molecular weight proteins in the supernatant from cells grown in dialysis tubing facilitated purification of the monoclonal antibodies.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D046508:Culture Techniques / Q000295:instrumentation* / Q000379:methods; D003956:Dialysis / Q000295:instrumentation; D006825:Hybridomas / Q000032:analysis / Q000276:immunology; D007074:Immunoglobulin G / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008970:Molecular Weight
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007075:Immunoglobulin M