Serum mediated changes in cell attachment and cytoplasmic organization of B16 melanoma correlates with selective alterations in secreted proteins.
B16 melanoma cells exhibited decreased adhesion to substration and the presence of cytoplasmic granules, resembling lipid inclusions, when cultured in vitro in the presence of syngenic serum from C57/BL6 mice. This constrasted with the rapid cell attachment and absence of cytoplasmic granules in cultures seeded in medium supplemented with an identical concentration of fetal bovine serum. Electrophoretic comparison of intracellular proteins revealed similar patterns in detergent-soluble and matrix-associated proteins from cells grown with bovine or mouse serum. However, a similar analysis of the conditioned media showed clear differences in methionine-rich species which migrated in the 100 kd region in cells grown with bovine serum, and as 110 kd component in cells grown with mouse serum. Our data indicate that the poor attachment to substratum and changes in cytoplasmic organization of B16 melanoma cells, is primarily associated with specific changes in secreted proteins.
D000818:Animals; D001769:Blood; D002448:Cell Adhesion; D002460:Cell Line; D003470:Culture Media; D003593:Cytoplasm / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D008545:Melanoma / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D008715:Methionine / Q000378:metabolism; D051379:Mice; D008854:Microscopy, Electron; D009363:Neoplasm Proteins / Q000302:isolation & purification / Q000378:metabolism*; D013462:Sulfur Radioisotopes
D003470:Culture Media; D009363:Neoplasm Proteins; D013462:Sulfur Radioisotopes; D008715:Methionine