Effect of benznidazole on the mixed function oxygenase system from rat liver microsomes.
Previous Benznidazole (Bz) administration to rats (30 mg/kg, i.p.) significantly prolongs their pentobarbital sleeping time. This prolonging effect of Bz administration correlates with the inhibitory action of Bz on the liver aminopyrine or ethylmorphine N-demethylase activities. Inhibition of these enzyme systems by Bz is non-competitive and would not be related to changes in liver microsomal cytochrome P-450 (P-450) content or in cytochrome c-reductase activity or to interactions of Bz with P-450 leading to spectral changes. Covalent interactions of Bz reactive metabolites with microsomal proteins or phospholipids might be involved instead.
D000633:Aminopyrine N-Demethylase / Q000378:metabolism; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005037:Ethylmorphine-N-Demethylase / Q000378:metabolism; D007700:Kinetics; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D009245:NADH Dehydrogenase / Q000378:metabolism; D009593:Nitroimidazoles / Q000494:pharmacology*; D010424:Pentobarbital / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012890:Sleep / Q000187:drug effects; D013997:Time Factors; D014344:Trypanocidal Agents / Q000494:pharmacology*
D009593:Nitroimidazoles; D014344:Trypanocidal Agents; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D000633:Aminopyrine N-Demethylase; D005037:Ethylmorphine-N-Demethylase; D009245:NADH Dehydrogenase; D010424:Pentobarbital; C009999:benzonidazole