Protein and nucleic acid analyses of influenza C viruses isolated from pigs and man.
The virus-coded proteins and the genomes of influenza C virus isolates obtained from Chinese pigs in 1981-1982 and of human influenza C virus strains isolated between 1947 and 1981 were compared. Using SDS polyacrylamide gel electrophoresis and one-dimensional peptide mapping we found the virus-coded proteins of the pig influenza C viruses to be similar to those of human influenza C virus strains. The sizes of the genomes of human and pig influenza C viruses were indistinguishable. Genome analysis by oligonucleotide (ON) mapping revealed that the genomes of the pig influenza C viruses were very similar to but not identical with those of human influenza C virus strains. ON changes were found scattered over the whole genome. ON mapping of isolated segments of several influenza C virus strains suggested that two pig strains (C/P/B/10/81 and C/P/B/32/81) are related by a reassortment event which is likely to have occurred in nature. The rate of genome variation in influenza C viruses seemed to be similar to that seen in influenza B, and slower than that recorded for influenza A viruses.
D000818:Animals; D005814:Genes, Viral; D014644:Genetic Variation; D006801:Humans; D009982:Gammainfluenzavirus / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D009843:Oligoribonucleotides / Q000302:isolation & purification; D009975:Orthomyxoviridae / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics; D013552:Swine / Q000382:microbiology; D014764:Viral Proteins / Q000032:analysis* / Q000235:genetics
D009843:Oligoribonucleotides; D012367:RNA, Viral; D014764:Viral Proteins