Cytotoxic effects of wheat gliadin-derived peptides.
The peptic-tryptic-cotazym (PTC) digest, obtained from bread wheat gliadin by simulating in vivo protein digestion, was more active than the PTC-digest of durum wheat gliadin in reversibly inhibiting HEp-2 cell proliferation and in increasing cellular acid phosphatase. Colony-forming ability of the cells was not affected by treatment with both bread or durum wheat gliadin peptides. The peptic-tryptic (PT) digest of bread wheat gliadin also showed agglutinating activity of HEp-2 cells.
D000135:Acid Phosphatase / Q000378:metabolism; D000371:Agglutination / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005903:Gliadin / Q000633:toxicity*; D006801:Humans; D066298:In Vitro Techniques; D010455:Peptides / Q000633:toxicity; D010940:Plant Proteins / Q000633:toxicity*; D014908:Triticum
D010455:Peptides; D010940:Plant Proteins; D005903:Gliadin; D000135:Acid Phosphatase