Double synchronization of human lymphocyte cultures: selection for high-resolution banded metaphases in the first and second division.
The present report describes a double synchronization technique that selects for first- and second-division metaphases in human lymphocyte cultures. The technique ensures high mitotic indices without elaborate handling of cultures. Combined with appropriate band induction, this technique provides a high yield of uniform metaphases with well-defined high-resolution banding.
D002478:Cells, Cultured; D002871:Chromosome Banding; D006801:Humans; D007621:Karyotyping; D008214:Lymphocytes / Q000166:cytology / Q000648:ultrastructure*; D008677:Metaphase