Three-dimensional structure of the complex of actin and DNase I at 4.5 A resolution.
The shape of an actin subunit has been derived from an improved 6 A map of the complex of rabbit skeletal muscle actin and bovine pancreatic DNase I obtained by X-ray crystallographic methods. The three-dimensional structure of DNase I determined independently at 2.5 A resolution was compared with the DNase I electron density in the actin:DNase map. The two structures are very similar at 6 A resolution thus leading to an unambiguous identification of actin as well as DNase I electron density. Furthermore the correct hand of the actin structure is determined from the DNase I atomic structure. The resolution of the actin structure was extended to 4.5 A by using a single heavy-atom derivative and the knowledge of the atomic coordinates of DNase I. The dimensions of an actin subunit are 67 A X 40 A X 37 A. It consists of a small and a large domain, the small domain containing the N terminus. Actin is an alpha,beta-protein with a beta-pleated sheet in each domain. These sheets are surrounded by several alpha-helices, comprising at least 40% of the structure. The phosphate peak of the adenine nucleotide is located between the two domains. The complex of actin and DNase I as found in solution (i.e., the actin:DNase I contacts which do not depend on crystal packing) was deduced from a comparison of monoclinic with orthorhombic crystals. Residues 44-46, 51, 52, 60-62 of DNase I are close to a loop region in the small domain of actin. At a distance of approximately 15 A there is a second contact in the large domain in which Glu13 of DNase I is involved. A possible binding region for myosin is discussed.
D000199:Actins / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D003850:Deoxyribonuclease I / Q000378:metabolism*; D046911:Macromolecular Substances; D008958:Models, Molecular; D009132:Muscles / Q000378:metabolism; D010179:Pancreas / Q000201:enzymology; D011487:Protein Conformation; D011817:Rabbits; D014961:X-Ray Diffraction / Q000379:methods
D000199:Actins; D046911:Macromolecular Substances; D003850:Deoxyribonuclease I