Resolution of cartilage proteoglycan and its proteolytic degradation products by high-performance liquid chromatography using a gel filtration system.
Cartilage proteoglycan subunits are resolved from their various-size proteolytic degradation products by a gel filtration high-performance liquid chromatography system using a Bio-Gel TSK-60 column in tandem with a Bio-Gel TSK-50 column. Molecules ranging in size from the intact proteoglycan to single chondroitin sulfate chains are eluted in the included volume. Each analysis takes less than 30 min to complete, and with purified samples as little as 20 micrograms of proteoglycan is required. The method can be applied to the measurement of proteoglycan in mixtures, such as tissue culture media, by monitoring effluent fractions using the dimethylmethylene blue dye-binding assay.
D000328:Adult; D000375:Aging; D000818:Animals; D002356:Cartilage / Q000378:metabolism*; D002358:Cartilage, Articular; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007231:Infant, Newborn; D009666:Nose; D011509:Proteoglycans / Q000032:analysis*
D011509:Proteoglycans