title: Differential inhibitors of DNA polymerases alpha and delta.
abstract: DNA polymerases alpha and delta from bone marrow are similar in many respects, the major known difference being the exonuclease activity of delta. Differential inhibitors of alpha and delta have been sought to assist in their functional and physical separation. Butylphenyl deoxyguanosine triphosphate is one. It effectively inhibits alpha at less than 1 microM concentration, whereas more than 100 microM is required to similarly inhibit delta. Another is the monoclonal antibody, SJK 132-20, which neutralizes the polymerase activity of alpha but not delta. These differential inhibitors further define alpha and delta as separate categories of eukaryotic DNA polymerase and promise to facilitate the study of both.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001853:Bone Marrow / Q000201:enzymology; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors* / Q000276:immunology; D004258:DNA Polymerase III; D003848:Deoxyguanine Nucleotides / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D019384:Nucleic Acid Synthesis Inhibitors; D011817:Rabbits; D013379:Substrate Specificity
chemical_list: D000911:Antibodies, Monoclonal; D003848:Deoxyguanine Nucleotides; D019384:Nucleic Acid Synthesis Inhibitors; C040364:N(2)-(4-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate; D004257:DNA Polymerase II; D004258:DNA Polymerase III