A novel glycosyl-amino acid linkage: rabbit-muscle glycogen is covalently linked to a protein via tyrosine.
A recent review summarizes our identification in rabbit-muscle glycogen of a protein that resists all attempts at removal by means that should displace non-covalently bound protein [Kennedy et al. (1985) In Membranes and Muscle (Berman, M.C., Gevers, W. and Opie, L.H. eds.) pp. 65-84, ICSU Press/IRL Press, Oxford]. Here we confirm that the glycogen is covalently bonded to the protein and report that the attachment is via a novel glycosidic linkage involving the hydroxyl group of tyrosine.
D000818:Animals; D002236:Carbohydrate Conformation; D006003:Glycogen / Q000032:analysis* / Q000096:biosynthesis; D006868:Hydrolysis; D009132:Muscles / Q000032:analysis*; D010766:Phosphorylation; D011402:Pronase; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011506:Proteins / Q000302:isolation & purification*; D011817:Rabbits; D014443:Tyrosine / Q000378:metabolism
D011506:Proteins; D014443:Tyrosine; D006003:Glycogen; D011505:Protein-Tyrosine Kinases; D011402:Pronase