Metabolic profiling of opioid peptides in tooth pulp by HPLC and radioreceptor assay.
A analytical system using a combination of gradient RP-HPLC and radioreceptor assay as the HPLC-detector is used to analyze the peptide-rich fraction extracted from a canine tooth pulp homogenate and to provide a metabolic profile of endogenous receptoractive peptides. The gradient RP-HPLC effectively separates the endogenous peptide mixture into a range of hydrophobicities that corresponds to a spectrum of peptide sizes. The receptor preparation is derived from a canine limbic system synaptosome fraction. 3H-DADL serves as the ligand in the RRA. The RP-HPLC/RRA data indicate canine tooth pulp contains a wide range of peptides that interact with the opioid peptide receptor preparation and displace the delta-receptor preferring ligand D2-ala, D5-Leu leucine enkephalin.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003782:Dental Pulp / Q000378:metabolism*; D004285:Dogs; D004723:Endorphins / Q000378:metabolism*; D008032:Limbic System / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D011869:Radioligand Assay; D013574:Synaptosomes / Q000378:metabolism; D013696:Temperature
D004723:Endorphins; D009419:Nerve Tissue Proteins