[Activation of pancreatic lipase by detergents. III. Oligomerization of the enzyme].
Activation of pancreatic lipase by non-micellar solution of sodium dodecylsulphate (SDS) has been studied. By means of gel-filtration it was found that SDS forces the lipase to form an octamer. A new method of the active sites titration using alkylboronic acids is proposed. The octameric form of the lipase was shown to contain six active sites at the optimal SDS concentration. The activated form of pancreatic lipase supposedly contains six native subunits, each of them forming an active site, and two conformationally altered subunits. This model was confirmed by a probability-theoretic calculation.
D000818:Animals; D001665:Binding Sites; D003902:Detergents / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D066298:In Vitro Techniques; D008049:Lipase / Q000378:metabolism*; D046911:Macromolecular Substances; D010179:Pancreas / Q000201:enzymology*; D011487:Protein Conformation; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology*
D003902:Detergents; D046911:Macromolecular Substances; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D008049:Lipase
Aktivatsiia pankreaticheskoĭ lipazy detergentami. III. Oligomerizatsiia fermenta.