Acid DNAase activity from Dictyostelium discoideum.
We have isolated and partially characterized an acid endonuclease activity from the cellular slime mold, Dictyostelium discoideum. This activity comprises more than 90% of the nonspecific DNA-endonuclease activity of the vegetative cells. Its molecular weight is about 44 000, and its activity is enhanced 7-fold by Mg2+. The pH optimum for the nicking activity depends upon NaCl concentrations, being at pH 5.0 in 207 mM NaCl, and at pH 5.8 in 7 mM NaCl. Large quantities of this enzyme activity are released into the growth medium or buffer, with detectable amounts appearing within 15 min of incubation.
D004023:Dictyostelium / Q000201:enzymology*; D004706:Endodeoxyribonucleases / Q000302:isolation & purification* / Q000378:metabolism; D005656:Fungal Proteins / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000378:metabolism; D008970:Molecular Weight; D009994:Osmolar Concentration; D012965:Sodium Chloride
D005656:Fungal Proteins; D012965:Sodium Chloride; D004706:Endodeoxyribonucleases; D008274:Magnesium