Amino acid transport by prosthecae of Asticcacaulis biprosthecum: evidence for a broad-range transport system.
Prosthecae purified from cells of Asticcaulis biprosthecum possess active transport systems that transport all 20 amino acids tested. Using ascorbate-reduced phenazine methosulphate in the presence of oxygen, all 20 amino acids are accumulated against a concentration gradient by isolated prosthecae. Results of experiments testing the inhibition of transport of one amino acid by another, and of experiments testing the exchange of exogenous amino acids with those preloaded in prosthecae, along with characteristics of mutants defective in amino acid transport, suggest the presence in prosthecae of three amino acid transport systems. One, the general or G system, transports at least 18 of the 20 amino acids tested. Another system, referred to as the proline or P system, transports seven amino acids (including proline) that are also transported by the G system. The third system transports only glutamate and aspartate, and is referred to as the acidic amino acid transport system or A system.
D000596:Amino Acids / Q000378:metabolism*; D001345:Autoradiography; D001692:Biological Transport; D002855:Chromatography, Thin Layer; D005998:Glycine / Q000378:metabolism; D006639:Histidine / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008715:Methionine / Q000378:metabolism; D009154:Mutation; D011392:Proline / Q000378:metabolism; D011548:Pseudomonadaceae / Q000378:metabolism*; D013696:Temperature
D000596:Amino Acids; D006639:Histidine; D011392:Proline; D008715:Methionine; D005998:Glycine