Uptake of [3H]2-deoxy-D-glucose and [3H]dopamine in adrenal chromaffin cells of the mouse.
Deoxyglucose method was applied to adrenal chromaffin cells. It was shown that dichromate-fixation could fix [3H]deoxyglucose in tissue sections. Dichromate-fixation has two advantages as compared with glutaraldehyde-fixation: (1) two types of adrenal chromaffin cells, adrenaline-storing (A) cell and noradrenaline-storing (NA) cells, are distinguishable, (2) more [3H]deoxyglucose is retained in sections than in those fixed with glutaraldehyde. No difference was demonstrated in the uptake of deoxyglucose between A and NA cells while A cells incorporated more [3H]dopamine than NA cells. This indicates that such difference was not due to the different rate of glucose utilization.
D000818:Animals; D001345:Autoradiography; D002838:Chromaffin System / Q000166:cytology / Q000378:metabolism*; D003837:Deoxy Sugars / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D005404:Fixatives; D051379:Mice; D008815:Mice, Inbred Strains; D011192:Potassium Dichromate
D003837:Deoxy Sugars; D005404:Fixatives; D003847:Deoxyglucose; D011192:Potassium Dichromate; D004298:Dopamine