Induction of a passive cutaneous anaphylaxis inhibitory factor in mice.
The administration of a purified protein from Dactylis glomerata pollen, named Dactylis inhibitory protein (DIP), to Balb/C mice results in the production of a heat-resistant seric factor that blocks the passive cutaneous anaphylaxis (PCA) reaction in rat skin. This PCA inhibitory factor (PCA-IF) was purified following different purification procedures and analyzed in sodium dodecyl sulfate polyacrylamide gel. Amongst the array of bands detected it was possible to ascribe the inhibitory activity to a protein band of 76,000 daltons but only when it was associated either with another protein band of 69,000 daltons (obtained after a one-step purification procedure) or two other protein bands of 55,000 and 26,000 daltons (obtained after a three-step purification procedure). The inhibitory activity of the PCA-IF is present at a low level in normal Balb/C mice sera and is enhanced after DIP treatment of the mice.
D000818:Animals; D006358:Hot Temperature; D051379:Mice; D008807:Mice, Inbred BALB C; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects*; D006109:Poaceae; D011058:Pollen; D011935:Reagins / Q000037:antagonists & inhibitors; D012867:Skin / Q000276:immunology; D013491:Suppressor Factors, Immunologic / Q000096:biosynthesis* / Q000302:isolation & purification; D013997:Time Factors
D011935:Reagins; D013491:Suppressor Factors, Immunologic