High-performance liquid chromatographic assay for methotrexate utilizing a cold acetonitrile purification and separation of plasma or cerebrospinal fluid.
D000097:Acetonitriles; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008727:Methotrexate / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid; D013056:Spectrophotometry, Ultraviolet
D000097:Acetonitriles; D008727:Methotrexate; C032159:acetonitrile