Culture of atrial and ventricular cardiac muscle cells from the adult squirrel monkey Saimiri sciureus.
Atrial and ventricular cardiac muscle cells isolated from the adult squirrel monkey Saimiri sciureus were cultured and characterized by light and transmission electron microscopy (TEM). Freshly isolated cells were striated and cylindrical in shape and, when placed in culture, rounded up and lost their highly organized morphology. With prolonged time in culture, they spread out on the surface of the culture flask and reacquired many of the internal ultrastructural characteristics of their in vivo atrial and ventricular cardiac muscle cell counterparts. Autoradiographic experiments indicated that both atrial and ventricular myocytes synthesized DNA when grown in culture. In some binucleated atrial cells only one nucleus became labelled. These studies show that it is now possible to culture cardiac muscle cells isolated from an adult primate.
D008841:Actin Cytoskeleton / Q000648:ultrastructure; D000818:Animals; D002427:Cebidae; D002469:Cell Separation; D002478:Cells, Cultured; D004261:DNA Replication; D005260:Female; D006056:Golgi Apparatus / Q000648:ultrastructure; D006325:Heart Atria / Q000166:cytology; D006352:Heart Ventricles / Q000166:cytology; D008854:Microscopy, Electron; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D012453:Saimiri; D012519:Sarcoplasmic Reticulum / Q000648:ultrastructure