Covalent binding of styrene and styrene-7,8-oxide to plasma proteins, hemoglobin and DNA in the mouse.
The extent of covalent binding to plasma proteins, hemoglobin and guanine-N-7 in DNA was determined after intraperitoneal administration of radiolabelled styrene and styrene-7,8-oxide to mice. The degree of alkylation increased non-linearly with the dose. It was proportionally higher after the highest doses of styrene-7,8-oxide while the reverse was observed with respect to the ability of styrene to alkylate plasma proteins and DNA. Thus, a dose dependence was indicated in the elimination of both styrene and styrene-7,8-oxide. A comparison of the degree of alkylation of plasma proteins, hemoglobin and guanine-N-7 in DNA suggests that the two compounds are about equally effective as alkylating agents in vivo at moderate dose levels. At high doses styrene-7,8-oxide is the more effective alkylator. The alkylation of DNA in liver, brain and lung after administration of styrene-7,8-oxide exceeded that in spleen and testis.
D000477:Alkylating Agents / Q000378:metabolism; D000478:Alkylation; D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004852:Epoxy Compounds / Q000378:metabolism*; D004988:Ethers, Cyclic / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D020058:Styrene; D013343:Styrenes / Q000378:metabolism*
D000477:Alkylating Agents; D001798:Blood Proteins; D004852:Epoxy Compounds; D004988:Ethers, Cyclic; D006454:Hemoglobins; D013343:Styrenes; D020058:Styrene; D004247:DNA; C013690:styrene oxide