Chloroplast biogenesis: quantitative determination of monovinyl and divinyl Mg-protoporphyrins and protochlorophyll(ides) by spectrofluorometry.
General equations which permit the determination of the amounts of any two closely related fluorescent compounds which can be distinguished by 77 degrees K but not by 293 degrees K spectrofluorometry have been described. This was achieved in the presence or absence of a third interfering compound, without prior separation of the fluorescent species. The adaptation of the generalized equations to the determination of the amounts of monovinyl (MV) and divinyl (DV) Mg-protoporphyrins or of MV and DV protochlorophyll(ides) in the presence or absence of Mg-Protos [Mg-protoporphyrin IX (Mg-Proto), Mg-Proto monoester, Mg-Proto diester or a mixture of those three tetrapyrroles] interference, was then demonstrated over a wide range of MV/DV tetrapyrrole proportions. These equations are likely to be very useful for the study of the intermediary metabolism of the monovinyl and divinyl chlorophyll biosynthetic routes in plants.
D002734:Chlorophyll / Q000031:analogs & derivatives*; D002736:Chloroplasts / Q000378:metabolism*; D008433:Mathematics; D010788:Photosynthesis; D010944:Plants / Q000032:analysis; D011166:Porphyrins / Q000032:analysis*; D011521:Protochlorophyllide / Q000031:analogs & derivatives* / Q000032:analysis*; D011524:Protoporphyrins / Q000032:analysis*; D011758:Pyrroles / Q000032:analysis; D013050:Spectrometry, Fluorescence; D013696:Temperature; D045725:Tetrapyrroles
D011166:Porphyrins; D011524:Protoporphyrins; D011758:Pyrroles; D045725:Tetrapyrroles; D002734:Chlorophyll; C039629:magnesium protoporphyrin; C020301:2,4-divinylprotochlorophyllide; D011521:Protochlorophyllide; C049198:magnesium monovinylprotoporphyrin