The degradation of beta-sitosterol by Pseudomonas sp. NCIB 10590 under aerobic conditions.
The bacterial degradation of beta-sitosterol by Pseudomonas sp NCIB 10590 has been studied. Major biotransformation products included 24-ethylcholest-4-en-3-one, androsta-1,4-diene-3,17-dione, 3-oxochol-4-en-3-one-24-oic acid and 3-oxopregn-4-en-3-one-20-carboxylic acid. Minor products identified were 26-hydroxy-24-ethylcholest-4-en-3-one, androst-4-ene-3,17-dione, 3-oxo-24-ethylcholest-4-en-26-oic acid, 3-oxochola-1,4-dien-3-one-24-oic acid, 3-oxopregna-1,4-dien-3-one-20 carboxylic acid and 9 alpha-hydroxyandrosta-1,4-diene-3,17-dione. Studies with selected inhibitors have enabled the elucidation of a comprehensive pathway of beta-sitosterol degradation by bacteria.
D000433:1-Propanol / Q000494:pharmacology; D000332:Aerobiosis; D001711:Biotransformation; D055598:Chemical Phenomena; D002621:Chemistry; D005285:Fermentation; D011549:Pseudomonas / Q000378:metabolism*; D012855:Sitosterols / Q000378:metabolism*
D012855:Sitosterols; C025473:gamma-sitosterol; D000433:1-Propanol