Methods for study of mutations and mutagenesis in human lymphocytes.
Detailed methods are presented for measurement and study of in vivo mutations and in vitro mutagenesis in human lymphocytes. The methods described include preparation of conditioned medium containing interleukin-2, enumeration of mutant clones, in vitro mutagenesis, and expansion of mutant clones for further study.
D002999:Clone Cells; D003470:Culture Media; D006801:Humans; D008214:Lymphocytes / Q000166:cytology*; D009152:Mutagenicity Tests / Q000379:methods*; D009154:Mutation; D010835:Phytohemagglutinins / Q000494:pharmacology
D003470:Culture Media; D010835:Phytohemagglutinins