[Evaluation of the immunospecific activity of allergens prepared from house dust and the mite Dermatophagoides pteronyssinus in the bacteriosorption reaction on a flat surface].
The content of protein A-reactive IgG to allergens prepared from house dust and D. pteronyssinus was determined in the sera of 4 immunized rabbits, 10 sensitized and hyposensitized guinea pigs and 37 treated and untreated allergic patients by means of the previously developed bacteriosorption test (BST). The sensitivity of the test for the determination of allergen-specific antibodies was 50-100 ng/ml. This test was shown to permit the detection of IgG in the sera of immunized, sensitized and hyposensitized animals, as well as in the sera of some treated and untreated patients. The antigenic similarity of both allergens was confirmed. Three batches of D. pteronyssinus allergen, standardized as to the content of protein nitrogen, differed in their immunospecific activity in BST with one of the rabbit sera and with the set of the patients' sera. The covalent immobilization of house-dust allergen on polystyrene by means of water-soluble carbodiimide gave no advantages in BST in comparison with adsorption immobilization in alkaline carbonate-bicarbonate buffer.
D000327:Adsorption; D000485:Allergens / Q000276:immunology*; D000818:Animals; D000941:Antigens / Q000276:immunology*; D004391:Dust; D006168:Guinea Pigs; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D066298:In Vitro Techniques; D008925:Mites / Q000276:immunology*; D011817:Rabbits; D012130:Respiratory Hypersensitivity / Q000175:diagnosis / Q000276:immunology*; D013205:Staphylococcal Protein A / Q000276:immunology; D013211:Staphylococcus aureus; D013499:Surface Properties
D000485:Allergens; D000941:Antigens; D004391:Dust; D007106:Immune Sera; D013205:Staphylococcal Protein A
Otsenka immunospetsificheskoĭ aktivnosti allergenov domashcheĭ pyli i kleshcha Dermatophagoides pteronyssinus v reaktsii bakteriosorbtsii na ploskoĭ poverkhnosti.