Secretogranins I and II: two tyrosine-sulfated secretory proteins common to a variety of cells secreting peptides by the regulated pathway.
We report on the biochemical and immunological properties as well as on the cellular and subcellular distribution of two proteins, called secretogranins I and II. These proteins specifically occur in a wide variety of endocrine and neuronal cells that package and sort regulatory peptides into secretory granules. Both secretogranins take the same intracellular route as the peptides and are also sorted into secretory granules. Secretogranins I and II are biochemically and immunologically distinct proteins and differ from chromogranin A. Yet, these three proteins are similar to each other in many respects and therefore constitute one class of proteins. A remarkable feature of this protein class is a very acidic pI, brought about by a high content of acidic amino acids as well as by phosphorylation on serine and sulfation on tyrosine and O-linked carbohydrate. As a result, this class of proteins has a high net negative charge even at the acidic pH of the trans Golgi cisternae. We discuss the possibility that this property of the proteins may point to a role in the packaging of regulatory peptides into secretory granules.
D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000313:Adrenal Medulla / Q000032:analysis* / Q000166:cytology; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002460:Cell Line; D053379:Chromogranin A; D002864:Chromogranins / Q000032:analysis; D009474:Neurons / Q000032:analysis; D010673:Pheochromocytoma / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000032:analysis* / Q000166:cytology; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification*; D051381:Rats; D013329:Structure-Activity Relationship
D000596:Amino Acids; D053379:Chromogranin A; D002864:Chromogranins; D011506:Proteins