Determination of nucleotide pools in plant tissue by high-performance liquid chromatography.
A procedure for the determination of nucleotide pools in plant tissue by HPLC is described. Sample preparation includes the extraction with 0.4 M HClO4, a purification step, which proved to be essential, on a disposable prepacked phenyl-bonded column, neutralization by KOH, and concentration by freeze-drying. The determination of a broad spectrum of ribonucleotides including the ribonucleosides was performed by combining anion-exchange and reversed-phase HPLC. Data are presented for suspension-cultured cells of Nicotiana tabacum and Datura innoxia and for the leaf and root of tobacco.
D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D013286:Datura stramonium / Q000032:analysis; D009711:Nucleotides / Q000032:analysis* / Q000302:isolation & purification; D010944:Plants / Q000032:analysis*; D010946:Plants, Medicinal; D010947:Plants, Toxic; D014026:Nicotiana / Q000032:analysis
D009711:Nucleotides