Gas chromatographic assay with pharmacokinetic applications for monitoring T-2 and HT-2 toxins in plasma.
A gas-liquid chromatographic (GLC) method for monitoring T-2 and HT-2 toxins in plasma was developed. The procedure involved extraction of the toxins with ethyl acetate, chromatography on a C18 reversed-phase column and derivatization with heptafluorobutyric anhydride (HFBA). The T-2 and HT-2 HFBA derivatives were chromatographed on OV-17 at various temperatures and measured with an electron-capture detector. Iso-T-2 toxin and iso-HT-2 toxin were used as internal standards. Recoveries averaged 95.1 +/- 8.6% for T-2 toxin and 102.1 +/- 5.2% for HT-2 toxin at levels ranging from 40 to 120 ng/ml. The limits of detection were 30 and 5 ng/ml of T-2 and HT-2 toxin, respectively. The range of the assay covers plasma concentrations at which toxicity becomes manifest. The pharmacokinetic application of this GLC method is illustrated by simultaneous monitoring of T-2 and HT-2 toxins levels in plasma obtained after intravenous administration of T-2 toxin to a dog.
D000818:Animals; D002849:Chromatography, Gas; D004285:Dogs; D004355:Drug Stability; D007700:Kinetics; D012717:Sesquiterpenes / Q000097:blood*; D013605:T-2 Toxin / Q000031:analogs & derivatives / Q000097:blood*
D012717:Sesquiterpenes; D013605:T-2 Toxin; C012351:HT-2 toxin