Cytogenetic characterization of the L5178Y TK+/-3.7.2C mouse lymphoma cell line.
The cytogenetic characterization of the L5178Y TK+/-3.7.2C mouse lymphoma cell line was carried out, utilizing G-banded metaphase chromosomes, to provide a karyotypic basis for the precise delineation of induced rearrangements in TK-/- mutants. Band-pattern measurements were used to construct ideograms which represent the position, number, size and staining intensity of the chromosome bands. The TK+/-3.7.2C cell line has been shown to provide quantitation of forward mutations induced at the autosomal thymidine kinase (TK) locus in this cell line. Chromosome analysis of the TK+/-3.7.2C cell line and derived TK-/- mutants has become important in demonstrating that the TK+/-----TK-/- assay may detect and distinguish between chromosomal events and smaller, perhaps point-mutation, events in mutant colonies.
D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D007621:Karyotyping; D008223:Lymphoma / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D051379:Mice; D009152:Mutagenicity Tests / Q000592:standards; D013937:Thymidine Kinase / Q000235:genetics*
D013937:Thymidine Kinase