Measurement of squirrel monkey serum IgG levels by a two-site sandwich radioimmunoassay with monoclonal antibodies.
Monoclonal antibodies against the squirrel monkey Saimiri sciureus IgG have been produced for a more specific analysis of the antibody-related immunological aspects in experimental human or monkey malaria. Two monoclonal antibodies, 3D8/D5 and 3F11/G10, out of 64 reacted with distinct epitopes on the IgG present throughout the complete population without interfering with each other. The 2 monoclonal antibodies were used to develop a highly specific, reliable and sensitive two-site sandwich radioimmunoassay for the measurement of the serum IgG levels in 83 animals. The antibodies also allowed us to produce by a simple immunoabsorbent technique a highly purified IgG standard easy to calibrate and store. The assay permits the detection of IgG levels as low as 0.48 ng/ml. The standard curve is linear between 3.9 and 125 ng protein/ml and allows by a simple mathematical equation an accurate measurement of the serum IgG levels.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002427:Cebidae / Q000276:immunology*; D002846:Chromatography, Affinity; D004306:Dose-Response Relationship, Immunologic; D007074:Immunoglobulin G / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods*; D012015:Reference Standards; D012453:Saimiri / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G