title: Human placental aldehyde dehydrogenase. Subcellular distribution and properties.
abstract: Freshly obtained human term placentae were subjected to subcellular fractionation to study the localization of NAD-dependent aldehyde dehydrogenases. Optimal conditions for the cross-contamination-free subcellular fractionation were standardized as judged by the presence or the absence of appropriate marker enzymes. Two distinct isozymes, aldehyde dehydrogenase I and II, were detected in placental extracts after isoelectric focusing on polyacrylamide gels. Based on a placental wet weight, about 80% of the total aldehyde dehydrogenase activity was found in the cytosolic acid and about 10% in the mitochondrial fraction. The soluble fraction (cytosol) contained predominantly aldehyde dehydrogenase II which has a relatively high Km (9 mmol/l) for acetaldehyde and is strongly inhibited by disulfiram. The results indicate that cytosol is the main site for acetaldehyde oxidation, but the enzyme activity is too slow to prevent the placental passage of normal concentrations of blood acetaldehyde (less than 1 mumol/l) produced by maternal ethanol metabolism.
mesh_terms: D000079:Acetaldehyde / Q000097:blood; D000428:Alcohol Drinking; D000444:Aldehyde Dehydrogenase / Q000378:metabolism*; D003600:Cytosol / Q000201:enzymology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D008431:Maternal-Fetal Exchange; D008928:Mitochondria / Q000201:enzymology; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D013347:Subcellular Fractions / Q000201:enzymology
chemical_list: D007527:Isoenzymes; D000444:Aldehyde Dehydrogenase; D000079:Acetaldehyde