title: Aromatase, estrogen 2-hydroxylase, and catechol-O-methyltransferase activity in isolated, cultured gonadotropic cells of mature African catfish, Clarias gariepinus (Burchell).
abstract: Isolated gonadotropic cells were able to convert androstenedione into estrone. Subsequently, estrone could be metabolized via catecholestrone into 2-methoxyestrone. From these results it can be concluded that the gonadotropes of mature catfish contain the enzymes aromatase, estrogen 2-hydroxylase, and catechol-O-methyltransferase. The possible function of these enzymes in the steroid negative feedback regulation of gonadotropic hormone release is discussed.
mesh_terms: D000375:Aging; D000818:Animals; D001141:Aromatase / Q000378:metabolism*; D002394:Catechol O-Methyltransferase / Q000378:metabolism*; D002478:Cells, Cultured; D019363:Cytochrome P-450 CYP1A1; D005399:Fishes / Q000378:metabolism*; D008297:Male; D010902:Pituitary Gland / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development; D012741:Sexual Maturation; D013250:Steroid Hydroxylases / Q000378:metabolism*
chemical_list: D013250:Steroid Hydroxylases; D001141:Aromatase; D019363:Cytochrome P-450 CYP1A1; C020120:estrogen 2-hydroxylase; D002394:Catechol O-Methyltransferase