Actin-associated cell-surface glycoprotein from ascites cell microvilli: a disulfide-linked multimer.
Isolated microvilli of the MAT-C1 subline of the 13762 rat mammary adenocarcinoma contain a transmembrane complex composed of a cell surface, cytoskeleton-associated glycoprotein (CAG), actin, and a 58,000-dalton polypeptide (58K). The behavior of CAG has been studied by differential centrifugation and velocity sedimentation gradient centrifugation of detergent extracts of microvilli. CAG can be pelleted along with a fraction of the microvillar actin even in the presence of ionic detergents and under microfilament-depolymerizing conditions. By velocity sedimentation analysis CAG in Triton/PBS extracts sediments as a large, heterogeneous species (sedimentation coefficient greater than 25S). In Sarkosyl and sodium dodecyl sulfate (SDS) the size and heterogeneity are somewhat reduced. In SDS CAG sediments as a 20S species in the absence of mercaptoethanol and as a 5S species in the presence of mercaptoethanol. These results indicate that CAG is a disulfide-linked multimer in the microvillus membrane. We suggest that the stable multimeric structure of CAG permits it to act as the membrane association site for several microfilaments and plays an important role in the formation and stabilization of the microvillus structure.
D000199:Actins / Q000032:analysis*; D000818:Animals; D001201:Ascites / Q000378:metabolism; D001665:Binding Sites; D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D002621:Chemistry; D003598:Cytoskeletal Proteins / Q000032:analysis*; D003902:Detergents; D004220:Disulfides / Q000032:analysis; D005260:Female; D006023:Glycoproteins / Q000032:analysis*; D046911:Macromolecular Substances; D008325:Mammary Neoplasms, Experimental / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis*; D008871:Microvilli / Q000032:analysis; D009363:Neoplasm Proteins / Q000032:analysis*; D010084:Oxidation-Reduction; D011489:Protein Denaturation; D051381:Rats; D012995:Solubility
D000199:Actins; D003598:Cytoskeletal Proteins; D003902:Detergents; D004220:Disulfides; D006023:Glycoproteins; D046911:Macromolecular Substances; D008565:Membrane Proteins; D009363:Neoplasm Proteins