Mutagenicity of ultrasound in cultured mammalian cells.
Chinese hamster V79 cells were exposed in suspension to 1 MHz continuous wave ultrasound at spatial peak intensities of 7 and 35 W/cm2. Exposure durations were from 10 to 180 sec, so as to encompass the first decade of decreased survival at each intensity. Exposure at 35 W/cm2 resulted in the induction of 6-thioguanine-resistant mutants. The mutation frequency was approximately one-tenth that induced by x rays at similar survival levels. No mutagenesis was observed at 7 W/cm2. Results are consistent with free radical formation as the mechanism responsible for ultrasound-induced mutagenesis.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D002478:Cells, Cultured; D006224:Cricetinae; D004351:Drug Resistance; D005609:Free Radicals; D009152:Mutagenicity Tests; D009154:Mutation; D013866:Thioguanine / Q000494:pharmacology; D013997:Time Factors; D014465:Ultrasonics
D005609:Free Radicals; D013866:Thioguanine