title: Identification by monoclonal antibody of a major (28 kDa) surface membrane antigen of Schistosoma mansoni.
abstract: Monoclonal antibody M.1 was generated from mice immunized with membrane enriched extracts of mechanically transformed schistosomula. M.1 bound to the surface membranes of cercariae and young (0-24 h post-transformation) schistosomula but did not bind to older schistosomula or cultured worms. M.1 immunoprecipitated an antigen of approximate molecular weight 27-28 kDa from schistosomula. Experiments using metabolic labeling showed that the antigen was actively synthesized by developing schistosomula. Further M.1 immunoprecipitated a similar 27-28 kDa antigen from membrane-enriched extracts of miracidia, lung and adult worms as well as from schistosomula.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis* / Q000235:genetics; D001700:Biomphalaria; D002462:Cell Membrane / Q000276:immunology; D008715:Methionine / Q000378:metabolism; D051379:Mice; D008808:Mice, Inbred CBA; D008970:Molecular Weight; D012550:Schistosoma mansoni / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D008715:Methionine