The effect of dexamethasone on the synthesis of collagen in normal human trabecular meshwork explants.
This study demonstrates that the trabecular meshwork cells of the human eye incorporate 3H-Proline into collagen during in vitro incubation. Addition of dexamethasone to the incubation mixture produced a marked decrease in this incorporation. Dexamethasone was active at 10(-8) M and higher concentrations. The specificity of the hormone effect was demonstrated by its inability to alter 3H-leucine incorporation in these cells. These results indicate that dexamethasone decreases the synthesis of collagen, a major component of the extracellular matrix, in the trabecular meshwork.
D003094:Collagen / Q000096:biosynthesis*; D003907:Dexamethasone / Q000494:pharmacology*; D006801:Humans; D011392:Proline / Q000378:metabolism; D014129:Trabecular Meshwork / Q000187:drug effects / Q000378:metabolism*; D014316:Tritium
D014316:Tritium; D003907:Dexamethasone; D003094:Collagen; D011392:Proline