title: Intracellular pH during ischemia in skeletal muscle: relationship to membrane potential, extracellular pH, tissue lactic acid and ATP.
abstract: Intracellular pH (pHi) was measured with double-barrelled microelectrodes during 4 h of complete tourniquet ischemia in rabbit gastrocnemius muscle (group I). The pHi was related to extracellular pH (pHe), membrane potential (Em), tissue lactic acid (LA) and ATP. A fall in pHi from 7.00 +/- 0.03 to 6.60 +/- 0.05 occurred during 4 h of ischemia, with a slight pH-drop (0.07 pH units) during the initial hour and a more pronounced drop of 0.13 pH units during the last hour of ischemia. These changes were paralleled by a considerable decrease in pHe from 7.30 +/- 0.01 to 6.36 +/- 0.05 and a sixfold increase of tissue LA. The buffering capacity during the 4 h of ischemia was estimated to 81.9 +/- 5.6 mmol H+/l X pH. In parallel with the reduction in pHe, the resting membrane potential decreased from -90 mV and stabilised at around -60 mV after 2 h of ischemia. A less negative cell interior would favour H+ extrusion since the Em-EH+ gradient was unchanged at about -70 mV during the entire period of ischemia. This could contribute to muscle fiber buffering during ischemia. In another set of experiments (group II) the muscular glycogen reserve was reduced 20 min prior to a 4 h period of ischemia. Thereby an ischemic state was created where ATP levels decreased to 30% of initial, in contrast to the unaltered ATP content in group I.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001826:Body Fluids / Q000502:physiology*; D005110:Extracellular Space / Q000502:physiology*; D006003:Glycogen / Q000172:deficiency; D006863:Hydrogen-Ion Concentration; D007424:Intracellular Fluid / Q000502:physiology*; D007511:Ischemia / Q000378:metabolism / Q000503:physiopathology*; D007773:Lactates / Q000378:metabolism*; D008297:Male; D008564:Membrane Potentials; D009132:Muscles / Q000098:blood supply* / Q000378:metabolism / Q000503:physiopathology; D011817:Rabbits
chemical_list: D007773:Lactates; D000255:Adenosine Triphosphate; D006003:Glycogen