Synthesis of the C-terminal octapeptide of pig oxyntomodulin. Lys-Arg-Asn-Lys-Asn-Asn-Ile-Ala: a potent inhibitor of pentagastrin-induced acid secretion.
The synthesis of Lys-Arg-Asn-Lys-Asn-Asn-Ile-Ala representing the C-terminal octapeptide of oxyntomodulin isolated from pig intestine is described. Its structure was confirmed by its 360-MHz 1H NMR spectra. The octapeptide was tested for its ability to inhibit pentagastrin-induced acid secretion, in the anaesthetized rat, in the conscious rat with chronic gastric fistula, and in the conscious cat with gastric chronic fistula. The octapeptide inhibits pentagastrin-induced acid secretion in all three models. Compared to oxyntomodulin, the parent hormone, the synthetic peptide was approximately 150 times less potent but has the same efficacy. Biological data are presented and discussed.
D000818:Animals; D002415:Cats; D005750:Gastric Juice / Q000378:metabolism; D005768:Gastrointestinal Hormones / Q000138:chemical synthesis*; D004763:Glucagon-Like Peptides / Q000138:chemical synthesis* / Q000494:pharmacology; D009842:Oligopeptides / Q000138:chemical synthesis*; D053772:Oxyntomodulin; D010418:Pentagastrin / Q000037:antagonists & inhibitors*; D010446:Peptide Fragments / Q000138:chemical synthesis*; D051381:Rats; D012636:Secretory Rate / Q000187:drug effects; D013552:Swine
D005768:Gastrointestinal Hormones; D009842:Oligopeptides; D053772:Oxyntomodulin; D010446:Peptide Fragments; D004763:Glucagon-Like Peptides; D010418:Pentagastrin