Simplification of 1H NMR spectra of proteins by one-dimensional multiple quantum filtration.
The use of multiple quantum filters for simplification and editing of one-dimensional 1H NMR spectra of proteins is demonstrated. Three, four and five quantum-filtered spectra have been recorded. The technique is applicable to proteins of molecular weight up to at least 18,000. Examples obtained for a mixture of amino acids and for the proteins plastocyanin and myoglobin are reported. A remarkable degree of spectral editing can be achieved by judicious choice of experimental parameters.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D007887:Fabaceae; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009211:Myoglobin / Q000032:analysis; D010946:Plants, Medicinal; D010970:Plastocyanin / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D014907:Whales
D000596:Amino Acids; D009211:Myoglobin; D011506:Proteins; D010970:Plastocyanin