Mechanism of self-protection in a puromycin-producing micro-organism.
Puromycin is a potent inhibitor of bacterial protein synthesis, but puromycin-producing Streptomyces alboniger KCC S-0309 is tolerant to the antibiotic in vivo. Puromycin bound to both 30S and 50S ribosomal subunits from S. alboniger and inhibited polyuridylate-directed polyphenylalanine synthesis by the ribosomes. However, the organism possessed a novel puromycin-inactivating enzyme which acetylated the antibiotic at the 2''-NH2 group of the O-methyltyrosine moiety.
D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism; D004352:Drug Resistance, Microbial; D009682:Magnetic Resonance Spectroscopy; D011691:Puromycin / Q000096:biosynthesis* / Q000494:pharmacology; D012270:Ribosomes / Q000187:drug effects; D013302:Streptomyces / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D013379:Substrate Specificity
D011691:Puromycin; D000123:Acetyltransferases