[Experimental evaluation of the pathogenicity of Lassa virus variants].
Pathogenicity for guinea pigs and white mice of various Lassa virus variants: native, having had 1 passage in Vero cell culture and 4 passages in newborn white mouse brain; a virus having gone through 10 passages in Vero cells and 8 mouse brain passages (variant No. 10); a small-plaque clone derived from variant No. 10 by the method of plaque-to-plaque cloning (variant No. 11k), was studied. Both the native virus and variant No. 10 were found to be similarly pathogenic for susceptible laboratory animals, while the small-plaque variant of Lassa virus became non-fatal for guinea pigs and white mice. The decline of pathogenic properties in variant No. 11k was shown not to be associated with the presence of temperature-sensitive mutants or defective interfering particles.
D000818:Animals; D000831:Animals, Newborn; D001116:Arenaviridae / Q000472:pathogenicity*; D003673:Defective Viruses / Q000472:pathogenicity; D014644:Genetic Variation; D006168:Guinea Pigs; D007836:Lassa virus / Q000472:pathogenicity*; D051379:Mice; D009154:Mutation; D010641:Phenotype; D013696:Temperature; D014762:Viral Interference; D010948:Viral Plaque Assay; D014774:Virulence; D014776:Virus Cultivation / Q000379:methods
Eksperimental'naia otsenka patogennosti variantov virusa Lassa.