Platelet storage in a plasma-free medium.
Currently, platelet concentrates are stored in 50 to 60 ml of plasma. A major drawback to storage in plasma is the considerable loss of platelet function which occurs during storage. A modified Tyrodes medium has been developed for storage of platelets. A comparison between platelet concentrates stored in this medium and in plasma showed that platelet aggregation and release responses to synergistic pairs of stimuli were equivalent for both types of concentrates on the day of preparation and after 72 hours. Platelet aggregation and release responses to single stimuli, the content of membrane glycoproteins, and the pH declined during storage but were similar for both preparations. The data show that plasma is not required to maintain in vitro platelet function during storage of platelet concentrates, but in vivo functions remain to be determined. The use of an artificial medium has the advantages of decreasing patient exposure to plasma contaminants, generating additional plasma for fractionation, and controlling more exactly the storage environment.
D001792:Blood Platelets; D001793:Blood Preservation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007552:Isotonic Solutions / Q000494:pharmacology; D007958:Leukocyte Count; D010949:Plasma / Q000502:physiology; D010952:Plasma Substitutes / Q000494:pharmacology; D010974:Platelet Aggregation; D010976:Platelet Count
D007552:Isotonic Solutions; D010952:Plasma Substitutes; C037692:Tyrode's solution