A new simple plastic chemotaxis device of the Boyden chamber type utilizing an immunoassay plate.
A new simple and economical plastic Boyden chamber for in vitro assay of leukocytes chemotaxis was devised by the use of a commercially available immunoassay plate. Zymosan-activated rat serum, formyl-methionyl-leucyl-phenylalanine and leukotriene B4 caused concentration-dependent migration of the leukocytes into the lower chamber. About 50% of the cells loaded in the upper chamber migrated into the lower chamber for 80 min at the optimal concentrations of the three chemoattractants tested.
D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D007118:Immunoassay / Q000295:instrumentation*; D066298:In Vitro Techniques; D008297:Male; D010969:Plastics; D051381:Rats; D011919:Rats, Inbred Strains; D015054:Zymosan / Q000494:pharmacology
D010969:Plastics; D015054:Zymosan