title: Ultrastructural immunocytochemical distribution of VIP-like immunoreactivity in dog ileum.
abstract: The electron-immunocytochemical protein A-gold technique was employed to study the subcellular localization of vasoactive intestinal polypeptide-like material in dog ileum. The vasoactive intestinal polypeptide-like immunoreactivity was found within a population of large granular vesicles similar in structure in nerve varicosities of the myenteric plexus, the deep muscular plexus, the submucous plexus, the longitudinal muscular layer and the mucosa; none was found in nerve cell bodies. In the myenteric plexus, submucous plexus, the mucosa and the longitudinal muscular layer, varicosities containing similar large granular vesicles consistently remained unstained suggesting that within these plexuses morphologically indistinguishable by our technique large granular vesicles are not necessarily biochemically identical. In the deep muscular plexus, nearly all varicosities with large granular vesicles contained immunoreactivity for vasoactive intestinal polypeptide, but these varicosities often contained a few unstained large granular vesicles. This suggests that vasoactive intestinal polypeptide may share the same varicosity or the same vesicle with other neuropeptides present in this plexus (e.g., substance P or enkephalins) and that this plexus is a site where vasoactive intestinal polypeptide exerts its control over motility.
mesh_terms: D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D004285:Dogs; D005260:Female; D007082:Ileum / Q000648:ultrastructure*; D007118:Immunoassay; D008297:Male; D008854:Microscopy, Electron; D014660:Vasoactive Intestinal Peptide / Q000032:analysis*
chemical_list: D000906:Antibodies; D000936:Antigen-Antibody Complex; D014660:Vasoactive Intestinal Peptide