Mitogenic inhibition and effect on survival of mice bearing L1210 leukemia using a combination of dehydroascorbic acid and hydroxycobalamin.
The present study was designed to test the effect of a combination of dehydroascorbic acid (DHA) and hydroxycobalamin (vitamin B12) on the survival of mice bearing L1210 leukemia. Results showed a significant increase in survival of treated mice compared with controls (p less than or equal to 0.0001) (Student's t-test). This positive effect was significantly lost when DHA was substituted by ascorbic acid (AA) in the same experimental conditions. In vitro findings also revealed that the DHA-B12 combination specifically inhibited mitoses of L1210 cells while non-neoplastic L929 cells were not affected.
D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D003683:Dehydroascorbic Acid / Q000008:administration & dosage; D004353:Drug Evaluation, Preclinical; D005260:Female; D006879:Hydroxocobalamin / Q000008:administration & dosage; D007939:Leukemia L1210 / Q000188:drug therapy* / Q000401:mortality; D051379:Mice; D008811:Mice, Inbred DBA
D006879:Hydroxocobalamin; D003683:Dehydroascorbic Acid