Construction of up-and-down temperature-jump apparatus.
A fast up-and-down temperature-jump apparatus whose dead time is about 60 ms was constructed. The principle of the method is to let the sample solution flow to the observation cell through a capillary in a heat-exchange chamber. Bubbling and cavitation effects in the observation cell at large up or down temperature jumps were eliminated by application of a nitrogen gas pressure of 2-5 bar. The down-temperature-jump method is especially effective for measuring temperature-induced conformational transitions of biopolymers and their assemblies.
D001704:Biopolymers; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D007700:Kinetics; D011487:Protein Conformation; D013696:Temperature; D013816:Thermodynamics
D001704:Biopolymers