High-performance liquid chromatographic method for the quantitation of a meta-O-dealkylated metabolite of flecainide acetate, a new antiarrhythmic.
A simple, sensitive and selective high-performance liquid chromatographic method has been developed to analyze meta-O-dealkylated flecainide, a major metabolite of flecainide acetate, in human biological fluids. Sample preparation is accomplished through the use of bonded-phase extraction columns and the samples are chromatographed on a reversed-phase system with fluorescence detection. An external calibration method is used for quantitation and the inter-day and intra-day precision and accuracy are good. The method has been used to determine metabolite levels in samples from healthy subjects and patients with arrhythmias or renal impairment.
D000889:Anti-Arrhythmia Agents / Q000032:analysis* / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D003640:Dealkylation; D003956:Dialysis; D005424:Flecainide / Q000031:analogs & derivatives; D006801:Humans; D010880:Piperidines / Q000032:analysis* / Q000097:blood*; D013997:Time Factors
D000889:Anti-Arrhythmia Agents; D010880:Piperidines; C044844:5-hydroxy-N-(2-piperidinylmethyl)-2-(2,2,2-trifluoroethoxy)benzamide; D005424:Flecainide