Intracellular free calcium localization in neutrophils during phagocytosis.
Intracellular free calcium (Ca2+ i) is thought to be an important second messenger for phagocyte functions. The fluorescent indicator Quin2 was used to measure and visualize [Ca2+]i in human neutrophils during chemotaxis toward, and phagocytosis of, opsonized zymosan. In neutrophils migrating toward zymosan, [Ca2+]i was highest in the lamellipodium. Neutrophils ingesting opsonized zymosan had the highest [Ca2+]i in the pseudopods and periphagosomal cytoplasm. Most of the increase in [Ca2+]i was from extracellular sources. Regional increments in [Ca2+]i are strategically located to modulate such cellular functions as chemotaxis, oxidative activity, and degranulation.
D000634:Aminoquinolines; D002118:Calcium / Q000502:physiology*; D002634:Chemotaxis, Leukocyte; D006801:Humans; D009504:Neutrophils / Q000378:metabolism / Q000502:physiology*; D010587:Phagocytosis; D015054:Zymosan
D000634:Aminoquinolines; D015054:Zymosan; C035739:Quin2; D002118:Calcium