Influence of temperature on the in vitro activity of GDP-mannose dolicholphosphate mannosyltransferase in rat and trout liver microsomes.
Temperature optimum of mannosyltransferase activity in liver microsomes is higher in trout than in rat, but this enzymatic activity for rat is higher than trout. Activation energies calculated for mannosyltransferase activity for trout and rat do not correlate with environmental temperature. For a given incubation temperature, Vm values for rat are higher than trout, whereas Km values for trout are lower than rat.
D000818:Animals; D004789:Enzyme Activation; D006602:Hexosyltransferases / Q000378:metabolism*; D007700:Kinetics; D008364:Mannosyltransferases / Q000378:metabolism*; D008862:Microsomes, Liver / Q000201:enzymology*; D051381:Rats; D013045:Species Specificity; D013696:Temperature; D013816:Thermodynamics; D014337:Trout
D006602:Hexosyltransferases; D008364:Mannosyltransferases; C022755:dolichyl-phosphate beta-D-mannosyltransferase