Synthesis of [2-11C]5,5-dimethyl-2,4-oxazolidinedione with and without added dimethyl carbonate as a carrier for studies with positron tomography.
Two methods were developed for the synthesis of [2-11C]5,5-dimethyl-2,4-oxazolidinedione ([2-11C]DMO) for use with positron emission tomography to measure regional cerebral tissue pH in vivo in man. In both methods, A and B, [2-11C]dimethyl carbonate (DMC) was prepared from [11C]phosgene and excess of sodium methoxide in methanol containing 2-hydroxyisobutyramide (HIBA). In method A, an excess of DMC was used as a carrier, while in method B none was used. In both methods, the [2-11C]DMC solution was then heated for 10 min at 150 degrees +/- 2 degrees C causing the reaction of [2-11C]DMC with HIBA to yield [2-11C]DMO with a radiochemical purity of greater than 99%. Method A gave significantly higher radioactive yields, a pure organic product, but lower specific activities. Flash chromatography was used for the separation and purification of [2-11C]DMO prepared by method B.
D001345:Autoradiography; D001921:Brain / Q000000981:diagnostic imaging*; D002250:Carbon Radioisotopes; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D004114:Dimethadione / Q000138:chemical synthesis*; D004583:Electrons; D006801:Humans; D007202:Indicators and Reagents; D007553:Isotope Labeling / Q000379:methods; D010080:Oxazoles / Q000138:chemical synthesis*; D011877:Radionuclide Imaging
D002250:Carbon Radioisotopes; D007202:Indicators and Reagents; D010080:Oxazoles; D004114:Dimethadione