Effect of subchronic cholestasis on microsomal mixed-function oxidases and the glutathione-conjugating enzyme system in rat liver.
Bile duct ligation in male rats for two weeks led to a marked increase in both serum sorbitol dehydrogenase activity and serum bile acid concentration indicating cholestatic liver injury. Furthermore, a rise in the hepatic hydroxyproline level indicating collagen accumulation was observed. As a consequence of these alterations, the hepatic microsomal mixed-function oxidase system was impaired as evidenced by a decrease in cytochrome P-450 content and in the activities of NADPH-cytochrome c reductase and aminopyrine-demethylase. While the hepatic glutathione content remained unaffected, the cytosolic GSH S-transferase activity was clearly suppressed due to subchronic cholestasis.
D000818:Animals; D002779:Cholestasis / Q000201:enzymology*; D005978:Glutathione / Q000032:analysis; D005982:Glutathione Transferase / Q000032:analysis*; D008099:Liver / Q000473:pathology; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D006899:Mixed Function Oxygenases; D005982:Glutathione Transferase; D005978:Glutathione