Evaluation of equilibrium gel filtration chromatography for the study of protein binding of aluminum in normal and uremic sera.
Normal and uremic serum samples were chromatographed on a gel filtration column and fractions were analyzed for protein and aluminum content. Whereas much of the aluminum in the serum samples eluted with protein, a significant fraction appeared to be associated with low molecular weight species. Analysis of column fractions for aluminum binding by a competitive binding assay indicated no aluminum-binding serum constituents in these later eluting fractions. Uremic serum appeared, however, to contain aluminum-binding constituents of a lower molecular weight than in normal serum.
D000535:Aluminum / Q000097:blood*; D001667:Binding, Competitive; D001798:Blood Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002850:Chromatography, Gel; D006801:Humans; D011485:Protein Binding; D012016:Reference Values; D014511:Uremia / Q000097:blood*
D001798:Blood Proteins; D000535:Aluminum