Modification of immune induction by adjuvant.
The role of adjuvanticity in the modification of the immune induction was studied by separate application of antigen and adjuvant in mice. An antigen specific plaque forming cell (PFC) test and a delayed type hypersensitivity (DTH) assay were used for these studies. Antigen (SRBC) and adjuvant were applied separately in time and place. A stable well defined water-in-oil emulsion without antigen was used as adjuvant. Our studies have shown that intraperitoneal (i.p.) injection of this adjuvant stimulates the intravenous (i.v.) induced PFC response, while the DTH response is suppressed. This effect can be demonstrated even when adjuvant has been injected i.p. 5 days prior to i.v. antigen injection. The stimulation of the PFC response depends on the dosage of i.v. injected antigen.
D000276:Adjuvants, Immunologic / Q000276:immunology*; D000818:Animals; D000917:Antibody Formation; D004306:Dose-Response Relationship, Immunologic; D004912:Erythrocytes / Q000276:immunology; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007111:Immunity, Cellular; D008297:Male; D051379:Mice; D013997:Time Factors
D000276:Adjuvants, Immunologic