The inhibitory effect of halides and carboxylates on hepatic NADH: cytochrome b5 oxidoreductase.
The inhibitory effect of several halides and carboxylates on bovine liver microsomal NADH: cytochrome b5 oxidoreductase (EC 1.6.2.2) was examined. The magnitude of inhibition was altered by various anion species but not by cation species. The order of effectiveness was F- less than acetate less than Cl- less than Br- less than I- less than succinate congruent to citrate. The kinetics of these inhibitions was competitive with cytochrome b5, and non-competitive with NADH, indicating that these anions inhibit the interaction of the enzyme with cytochrome b5.
D000818:Animals; D000838:Anions / Q000494:pharmacology*; D002264:Carboxylic Acids / Q000494:pharmacology*; D002417:Cattle; D003579:Cytochrome Reductases / Q000037:antagonists & inhibitors*; D042966:Cytochrome-B(5) Reductase; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D009243:NAD / Q000494:pharmacology
D000838:Anions; D002264:Carboxylic Acids; D009243:NAD; D003579:Cytochrome Reductases; D042966:Cytochrome-B(5) Reductase