In vivo transcription of ribosomal RNA in relation to the mitotic cycle in Physarum polycephalum.
We have investigated the transcription of ribosomal RNA in plasmodia of Physarum polycephalum by a combination of pulse-labelling with [3H]uridine and RNA:DNA hybridization. The DNA used for the hybridization was a HindIII restriction fragment (cloned in bacteriophage lambda) of Physarum ribosomal DNA that carries a substantial fraction of the rRNA genes, enabling the ribosomal transcripts in the newly synthesized RNA to be measured. We found that ribosomal RNA constituted about 40% of the pulse-labelled RNA at all times during the synchronous mitotic cycle.
D004247:DNA / Q000235:genetics; D006824:Hybridization, Genetic; D008938:Mitosis; D010804:Physarum / Q000235:genetics* / Q000378:metabolism; D012335:RNA, Ribosomal / Q000096:biosynthesis / Q000235:genetics*; D014158:Transcription, Genetic
D012335:RNA, Ribosomal; D004247:DNA