title: Interaction of heparin with multimolecular aggregates of acetylcholinesterase.
abstract: It has been reported previously that heparin, a sulfated glycosaminoglycan, releases the asymmetric 16 S form of acetylcholinesterase (AChE) from cholinergic synapses. Here it is shown that heparin releases the synaptic AChE not as individual 16 S species but as multimolecular aggregates (30 S) of such molecules. Heparin is able to convert low-ionic strength AChE aggregates into a heparin type of AChE aggregates. Our results suggest that the AChE aggregates detached by heparin are likely to be the physiologically important state of aggregation of the 16 S AChE form in the synaptic basal lamina.
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D004347:Drug Interactions; D006493:Heparin / Q000378:metabolism / Q000494:pharmacology*; D006497:Heparan Sulfate / Q000494:pharmacology; D046911:Macromolecular Substances; D009469:Neuromuscular Junction / Q000187:drug effects / Q000201:enzymology*; D051381:Rats
chemical_list: D000110:Acetylcholinesterase; D006493:Heparin; D006497:Heparan Sulfate; D046911:Macromolecular Substances