Replacement of methoxatin by 4,7-phenanthroline-5,6-dione and the inability of other phenanthroline quinones, as well as 7,9-di-decarboxy methoxatin, to serve as cofactors for the methoxatin-requiring glucose dehydrogenase of Acinetobacter calcoaceticus.
Glucose dehydrogenase from A. calcoaceticus has been dissociated into apoenzyme and methoxatin coenzyme, and enzyme activity restored by replacing coenzyme with 4,7-phenanthroline-5,6-dione but not with 1,10- nor 1,7-phenanthroline-5,6-diones nor with 7,9-decarboxy methoxatin.
D000150:Acinetobacter / Q000201:enzymology*; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D003067:Coenzymes / Q000494:pharmacology; D042843:Glucose 1-Dehydrogenase; D005948:Glucose Dehydrogenases / Q000378:metabolism*; D010084:Oxidation-Reduction; D045542:PQQ Cofactor; D010618:Phenanthrolines / Q000494:pharmacology*; D011804:Quinolines / Q000494:pharmacology*; D015363:Quinolones; D013329:Structure-Activity Relationship
D003067:Coenzymes; D010618:Phenanthrolines; D011804:Quinolines; D015363:Quinolones; D045542:PQQ Cofactor; C046805:7,9-di-decarboxy methoxatin; D002237:Carbohydrate Dehydrogenases; D005948:Glucose Dehydrogenases; D042843:Glucose 1-Dehydrogenase