The influence of antiplatelet drugs on injury-stimulated migration of cultured smooth muscle cells.
Smooth muscle cell (SMC) migration, one of the early events in arteriosclerotic lesion formation in vivo, can be triggered by balloon catheter injury. This accelerated migration persists when aortic explants are grown in tissue culture. Under low serum conditions (0.1% fetal bovine serum) virtually no smooth muscle cell migration occurs out of explants from sham operated control animals, while over 70% of explants from balloon injured rats show SMC migration. Since platelets are thought to contribute to SMC proliferation and migration, we tested the effect of different antiplatelet drugs (sulfinpyrazone, aspirin, dipyridamole, and a combination of the last two) on balloon injury stimulated SMC migration. One week after onset of drug treatment balloon catheterization was performed and SMC migration from aortic explants was assessed 4 days later in vitro. The time and number of explants showing SMC migration was recorded daily over 8 days. Sulfinpyrazone reduced stimulated SMC migration most effectively, followed by the combination of aspirin and dipyridamole, while aspirin or dipyridamole alone had no significant effect on stimulated SMC migration. Using this technique we were able to single out SMC migration and analyze the effect of antiplatelet drugs on this early step in arteriosclerotic lesion formation.
D000818:Animals; D001161:Arteriosclerosis / Q000209:etiology / Q000473:pathology*; D001241:Aspirin / Q000494:pharmacology*; D002404:Catheterization / Q000009:adverse effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D004176:Dipyridamole / Q000494:pharmacology*; D007016:Hypophysectomy; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000473:pathology*; D051381:Rats; D011919:Rats, Inbred Strains; D013442:Sulfinpyrazone / Q000494:pharmacology*
D004176:Dipyridamole; D001241:Aspirin; D013442:Sulfinpyrazone