[Selective cytotoxicity of an antibody-ricin A chain conjugate for human tumor cells].
The toxic subunit of a plant ricin has been conjugate by a disulfide bond to a polyclonal rabbit antibody specific for the L-chain of human IgG. Both the antibody and ricin A-chain retained their original biological activity after conjugation. This conjugate proved to be a potent cytotoxin for surface Ig positive Burkitt lymphoma EB-3 cells, growing in vitro and produced 50% inhibition of protein synthesis at level of 1.4 x 10(-9) M. When tested for cytotoxic action on target cells, the composite conjugate molecule was at least 100 times more effective than antibodies alone, ricin A-chain alone or a conjugate ricin A-chain--normal rabbit IgG.
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage*; D002051:Burkitt Lymphoma / Q000276:immunology / Q000378:metabolism*; D002474:Cell-Free System; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D014176:Protein Biosynthesis; D011817:Rabbits / Q000276:immunology; D012156:Reticulocytes / Q000378:metabolism; D012276:Ricin / Q000276:immunology / Q000494:pharmacology*
D000906:Antibodies; D007074:Immunoglobulin G; D009363:Neoplasm Proteins; D012276:Ricin
Izbiratel'naia tsitotoksichnost' kon''iugata antitelo-A-tsep' ritsina dlia opukholevykh kletok cheloveka.