title: Toxicological effects of gamma-irradiated sewage solids fed as seven percent of diet to sheep for four years.
abstract: Breeding ewes in drylot were fed pelleted complete diets with 3% cottonseed meal (CSM) or 7% dried, gamma-irradiated sewage solids (DGSS) for 4 yr. Cytochrome P-450 (P-450) content and enzyme activities for xenobiotics biotransformations were assayed in livers after 2 yr and in livers, kidneys and ileal tissue after 4 yr. Dietary DGSS caused no increase in P-450 and few changes in activities of oxidative, hydrolative and conjugative biotransformational enzymes. Consumption of DGSS for 4 yr caused slight enlargement of spleens (1.1-fold, P less than .10) and ovaries (1.3-fold, P less than .10), but no change in size of livers, kidneys, hearts, adrenals and thyroids (P greater than .10), nor liver vitamin A levels (P greater than .10). Of 22 refractory lipophilic residues assayed in abdominal adipose tissue, few were detected and of those detected DGSS caused none to exceed normal levels. Dietary DGSS increased (P less than .01) Fe in livers 1.5-fold and in spleens 5.6-fold, and increased Cu in livers 1.3-fold (P less than .01) and in kidneys 1.2-fold (P less than .05). Dietary DGSS increased Cd levels in livers (P less than .01) but not in kidneys or spleens (P greater than .10); yet all Cd levels were within ranges for livestock fed conventional feed. Dietary DGSS caused no increase (P greater than .10) in levels of Ag, Ca, Cr, Hg, K, Mg, Mn, Na, Ni, P, Pb or Zn in livers, kidneys or spleens. There were no histopathological lesions of toxicosis except mild hemosiderosis of spleens. Consumption of a diet with 7% DGSS throughout 4 yr caused no hazardous accumulation of toxic elements and little, if any, evidence of toxicosis.
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000821:Animal Feed; D000818:Animals; D001711:Biotransformation; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005720:Gamma Rays; D008099:Liver / Q000378:metabolism; D008670:Metals / Q000378:metabolism; D009929:Organ Size; D012722:Sewage; D012756:Sheep / Q000254:growth & development / Q000502:physiology*; D013997:Time Factors; D014801:Vitamin A / Q000378:metabolism
chemical_list: D008670:Metals; D012722:Sewage; D014801:Vitamin A; D003577:Cytochrome P-450 Enzyme System