title: Determination of HBV DNA in serum in a case of needlestick hepatitis.
abstract: HBV DNA in serum was determined by modified spot hybridization. A nurse of the dialysis staff was inoculated via needlestick with blood of a HBsAg-positive hemodialysis patient, who had 2000 pg HBV DNA per milliliter serum. After insufficient passive immunization the nurse developed transient anicteric hepatitis B. HBV DNA was positive in sera of the recipient before and at the beginning of the elevation of transaminases.
mesh_terms: D000328:Adult; D001483:Base Sequence; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D006508:Hepatitis Antibodies / Q000032:analysis; D006509:Hepatitis B / Q000276:immunology / Q000635:transmission*; D006511:Hepatitis B Antigens / Q000276:immunology; D006515:Hepatitis B virus / Q000276:immunology*; D006801:Humans; D007116:Immunization, Passive; D008297:Male; D008875:Middle Aged; D009339:Needles; D009784:Occupational Diseases / Q000276:immunology / Q000635:transmission*; D006435:Renal Dialysis
chemical_list: D004279:DNA, Viral; D006508:Hepatitis Antibodies; D006511:Hepatitis B Antigens