A rapid sample preparation method for the determination of sulfamethazine in swine tissues by high-performance liquid chromatography.
A rapid procedure for the determination of sulfamethazine in swine muscle and kidney is described. The method comprises sonication-aided extraction with dichloromethane, cleaning-up of the extract on a combination of silica and reversed-phase C18 Sep-Pak cartridges and analysis by high-performance liquid chromatography on a Hypersil ODS column using acetonitrile/ammonium acetate solution (10 mmol X 1(-1), pH 6.8) 1 + 3 v/v as the mobile phase. Detection was performed at 254 nm. Mean recoveries of added sulfamethazine from swine muscle and kidney, at levels of 0.05 to 0.5 micrograms X g-1, were 89.5 and 80.5%, respectively. After intramuscular injection of sulfamethazine into swine, residues in tissues were also determined. The application of ultrasonic energy for the extraction of sulfamethazine residues from animal tissues is also discussed.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007668:Kidney / Q000032:analysis; D008460:Meat / Q000032:analysis*; D009132:Muscles / Q000032:analysis; D013010:Sonication; D013418:Sulfamethazine / Q000032:analysis*; D013552:Swine
D013418:Sulfamethazine