Conjugation of organic compounds in isolated hepatocytes from a marine fish, the plaice, Pleuronectes platessa.
A method is described for the preparation of viable hepatocytes from a marine fish, the plaice. Their ability to detoxify organic compounds was measured by the formation of glucuronic acid and sulphate conjugates with the model substrates 1-naphthol and phenolphthalein. 1-Naphthol was conjugated three- to four-fold faster than phenolphthalein and glucuronidation predominated with both substrates. Strong substrate inhibition of glucuronidation was observed with 200 microM 1-naphthol or phenolphthalein. No measurable sulphate conjugation was detected with phenolphthalein. Treatment of fish with 3-methylcholanthrene induced formation of both glucuronide and sulphate conjugates by two- to three-fold. Compared with rat hepatocytes, the extent of sulphation was 100-fold lower in plaice hepatocytes whereas glucuronide formation was only 10-fold lower. The observations indicate that isolated plaice hepatocytes provide a suitable system for studies of the detoxication of xenobiotic pollutants in fish liver.
D000818:Animals; D002470:Cell Survival; D004785:Environmental Pollutants / Q000378:metabolism*; D005399:Fishes / Q000378:metabolism*; D005965:Glucuronates / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D009284:Naphthols / Q000378:metabolism; D020113:Phenolphthalein; D010635:Phenolphthaleins / Q000378:metabolism; D013464:Sulfuric Acids / Q000378:metabolism
D004785:Environmental Pollutants; D005965:Glucuronates; D009284:Naphthols; D010635:Phenolphthaleins; D013464:Sulfuric Acids; C029350:1-naphthol; D020113:Phenolphthalein