Tenacious binding of lipids to vimentin during its isolation and purification from Ehrlich ascites tumor cells.
Vimentin enriched in cytoskeletal frameworks by Triton X-100 extraction of Ehrlich ascites tumor cells and purified from a low ionic strength extract of the cell residues by (NH4)2SO4 precipitation and DEAE-Sepharose and ssDNA-cellulose chromatography in the presence of 6 M urea was highly contaminated with lipids. Thin-layer chromatography of a chloroform-methanol extract of the purified protein revealed, besides small amounts of phospholipids, the presence of large quantities of neutral lipids.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis*; D002499:Centrifugation, Density Gradient; D002725:Chloroform; D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D008055:Lipids / Q000302:isolation & purification*; D000432:Methanol; D051379:Mice; D010743:Phospholipids / Q000302:isolation & purification; D011485:Protein Binding; D014746:Vimentin / Q000302:isolation & purification*
D008055:Lipids; D010743:Phospholipids; D014746:Vimentin; D002725:Chloroform; D000432:Methanol