[HPLC method for determination of vitamin C in milk, whey and whey beverages].
A method is described for the estimation of L-ascorbic acid and L-dehydroascorbic acid in milk, whey, and whey drinks by paired-ion reversed-phase HPLC using a C18-column. L-ascorbic acid is detected UV-spectrophotometrically at 248 nm, L-dehydroascorbic acid is determined spectrofluorometrically with excitation at 350 nm and emission at 430 nm, after derivatisation into a quinoxaline fluorophor.
D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003611:Dairy Products / Q000032:analysis*; D003683:Dehydroascorbic Acid / Q000032:analysis; D008892:Milk / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D001205:Ascorbic Acid; D003683:Dehydroascorbic Acid
HPLC-Methode zur Bestimmung von Vitamin C in Milch, Molke und Molkegetränken.