High-performance liquid chromatographic analysis of pulmonary metabolites of Leu- and Met-enkephalins in isolated perfused rat lung.
A high-performance liquid radiochromatographic analytical system has been developed which allows the determination of [3H]Leuenkephalin, [3H]Met-enkephalin and their potential metabolites [3H]TyrGlyGlyPhe, [3H]TyrGlyGly, [3H]TyrGly and [3H]tyrosine. Using this procedure, the biotransformation of each of the above enkephalins after 20 min of recirculating transit through isolated perfused rat lungs resulted in the formation of two major metabolites: tyrosine and TyrGlyGly in each case. The results indicate that significant metabolism of enkephalins may occur in the pulmonary circulation.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004743:Enkephalin, Leucine / Q000378:metabolism*; D004744:Enkephalin, Methionine / Q000378:metabolism*; D066298:In Vitro Techniques; D008168:Lung / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013056:Spectrophotometry, Ultraviolet
D004744:Enkephalin, Methionine; D004743:Enkephalin, Leucine