[Gas chromatographic study of deoxynivalenol and nivalenol in cereals following derivatization with heptafluorobutyrates].
A method for the analysis of deoxynivalenol (DON) und nivalenol (NV) in cereals is described. This method is suitable for routine use for control purposes. The extract is purified on a florisil column. Derivatization with heptafluorobutyrylimidazole is followed by quantitative determination by means of capillary gas chromatography and electron capture detection (ECD). For verification purposes two-dimensional thin layer chromatography is applied to gc-positive extracts. 67 cereal samples and cereal products from the Berlin market place have been assayed. Depending on the type of sample the limit of detection was 20 to 30 micrograms/kg for DON, and 100 to 300 micrograms/kg for NV.
D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D002523:Edible Grain / Q000032:analysis*; D005466:Fluorocarbons; D005506:Food Contamination / Q000032:analysis*; D012717:Sesquiterpenes / Q000032:analysis*; D014255:Trichothecenes / Q000032:analysis*
D005466:Fluorocarbons; D012717:Sesquiterpenes; D014255:Trichothecenes; C033094:perfluorobutyric acid; C038405:nivalenol; C007262:deoxynivalenol
Gaschromatographische Untersuchung von Cerealien auf Deoxynivalenol und Nivalenol nach Derivatisierung zu den Heptafluorbutyraten.