High-performance liquid chromatographic studies on non-mercapt in equilibrium with mercapt conversion of human serum albumin. II.
High-performance liquid chromatographic (HPLC) analysis of human serum albumin (HSA) on a GS-520 column with 0.03 M sodium phosphate buffer-0.15 M sodium sulphate (pH 6.87) showed three peaks, the principal component corresponding to human mercaptalbumin (HMA) and the secondary and tertiary components to nonmercaptalbumin (HNA). Using HPLC analysis, the nonmercapt----mercapt conversion of HSA during haemodialysis and the mercapt----nonmercapt conversion after haemodialysis in chronic renal failure were re-confirmed, indicating that HMA is a covalent carrier protein for sulphur-containing amino acids. Fractions of HMA in various liver diseases were significantly lower than those of healthy male adults.
D000328:Adult; D002352:Carrier Proteins / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003553:Cystine / Q000097:blood; D006801:Humans; D007676:Kidney Failure, Chronic / Q000378:metabolism; D008107:Liver Diseases / Q000378:metabolism; D008297:Male; D006435:Renal Dialysis; D012709:Serum Albumin / Q000032:analysis*; D013438:Sulfhydryl Compounds / Q000032:analysis*
D002352:Carrier Proteins; D012709:Serum Albumin; D013438:Sulfhydryl Compounds; D003553:Cystine