[Centrioles and microtubules in interphase cells exposed to colcemid. The concentration- and time-dependent effect of the action of the poison].
Under the action of colcemid on SPEV cells the network of cytoplasmic microtubules disappears within less than 1 hour; microtubules attached to pericentriolar satellites are retained for 4 hours. The disassembly time of these microtubules does not depend on colcemide concentration. It is therefore assumed that most of the microtubules are not attached to the centrioles, but have two free ends, thus confirming a hypothesis that they are conveyer-assembled. With colcemid concentration equal to 0.5 mcg/ml, the following dynamics of events is observed for the cell centre: after the microtubules attached to the satellites had disappeared, clusters of electron dense material appear around the centrioles (6 hour incubation), then short microtubules occur among clusters (8 hour incubation) to be subsequently retained (up to 40 hour incubation).
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002502:Centrioles / Q000187:drug effects* / Q000648:ultrastructure; D003703:Demecolcine / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D007399:Interphase / Q000187:drug effects*; D008854:Microscopy, Electron; D008870:Microtubules / Q000187:drug effects* / Q000648:ultrastructure; D013552:Swine; D013997:Time Factors
D003703:Demecolcine
Tsentrioli i mikrotrubochki v interfaznykh kletkakh pri vozdeĭstvii koltsemida. Effekt, zavisimyĭ ot kontsentratsii i vremeni deĭstviia iada.