An evaluation of commercially available dehydrated Rappaport-Vassiliadis medium for the isolation of salmonellae from poultry.
A total of 745 samples of chicken giblets was cultured to determine the relative efficiency of a commercially available Rappaport-Vassiliadis medium (RV-Oxoid). Experiments to determine the optimum inoculation ratio showed that 1:100 was superior to the other ratios tested. Comparison of RV-Oxoid with standard RV and RV-medium prepared using soya peptone (RV-soya) showed that after 24 h RV-soya was significantly better than RV-Oxoid (P less than 0.05), although there was no significant difference between standard RV and RV-Oxoid. Furthermore, when the duration of incubation was extended to 48 h there was no significant difference between the three media (P greater than 0.25). We conclude that RV-Oxoid is a satisfactory product for the isolation of salmonellae from poultry, providing that it is inoculated at a ratio of 1:100 and is incubated for 48 h. Its use can therefore be recommended to laboratories who wish to use a dehydrated medium.
D000818:Animals; D003470:Culture Media; D008460:Meat / Q000032:analysis; D011200:Poultry / Q000382:microbiology*; D012475:Salmonella / Q000302:isolation & purification*
D003470:Culture Media