title: [Isozymes of NADP-dependent isocitrate dehydrogenase in normal and tumor tissues of various mouse strains and their hybrids].
abstract: Normal tissues of DBA, CBA, CC57W, C3H, Balb/c, SHR mice and F1 hybrids CC57W/DBA appeared to differ in the ratios of mitochondrial and supernatant NADP-dependent isocitrate dehydrogenase (IDH). Tested inbred mice strains CC57W, C3H, SHR, Balb/c contain allelic form Idh-1a of supernatant IDH gene Idh-1, whereas allelic form Idh-1b is characteristic of mice strains DBA and CBA. In tumors IDH isozymes have the same mobility as do isozymes of homologous normal tissues; but their activity is lower. A high variability of each isozyme activity in the isozyme spectrum is revealed in various tissues of F1 hybrids CC57W/DBA. Allelic forms of gene Idh-1 were used as markers of normal and tumor cells for the experimental model: transplantation of sarcoma 37 (Idh-1a/Idh-1a) to subcutaneous tissue of the mouse strain DBA (Idh-1b/Idh-1b). It enables us to reveal isozymes of stromal cell in tumor IDH isozyme spectrum. The results indicate that the relation of normal and tumor isozymes vary in different tumors.
mesh_terms: D000483:Alleles; D000818:Animals; D003433:Crosses, Genetic; D004591:Electrophoresis, Polyacrylamide Gel; D005796:Genes; D014644:Genetic Variation; D007521:Isocitrate Dehydrogenase / Q000032:analysis* / Q000235:genetics; D007527:Isoenzymes / Q000032:analysis* / Q000235:genetics; D008748:Methylcholanthrene; D051379:Mice; D008815:Mice, Inbred Strains / Q000378:metabolism*; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000201:enzymology*; D012208:Rhabdomyosarcoma / Q000201:enzymology
chemical_list: D007527:Isoenzymes; D008748:Methylcholanthrene; D007521:Isocitrate Dehydrogenase; C039355:isocitrate dehydrogenase (NADP+)
vernacular_title: Izozimy NADF-zavisimoĭ izotsitratdegidrogenazy v normal'nykh i opukholevykh tkaniakh mysheĭ razlichnykh liniĭ i ikh gibridov.