title: Simultaneous determination of epinephrine and norepinephrine in urine by derivative synchronous fluorescence spectroscopy.
abstract: The combination of synchronous and derivative fluorimetry, potentially useful in resolving mixtures that present strongly overlapping conventional fluorescence spectra, appears suitable for determining epinephrine and norepinephrine in urine. We describe a simple, rapid, and inexpensive method for simultaneous assay of these compounds in a single scan of urine specimens from normal adults. The catecholamines are extracted from the sample on cation-exchange columns and subsequently determined by formation of trihydroxyindoles. Analytical recovery is about 94% for epinephrine, 91% for norepinephrine, with a CV of approximately 5%. No sophisticated detection equipment is necessary, a conventional modern fluorimeter being adequate.
mesh_terms: D000328:Adult; D004837:Epinephrine / Q000652:urine*; D006801:Humans; D009638:Norepinephrine / Q000652:urine*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation
chemical_list: D009638:Norepinephrine; D004837:Epinephrine