title: [Alkaline phosphatase activity of normal and transformed cultured human cells].
abstract: A study was made of the relationship between the activity of alkaline phosphatase and the proliferation of cultured human cells with different replicative potentials. It is shown that alkaline phosphatase plays a role as one of endogenic stimulators of cellular proliferation. The ageing of diploid cells is accompanied by a decrease in the enzyme activity. Maximum activity was observed during a period of logarithmic cell growth. Addition of placental alkaline phosphatase to the synchronized diploid cells stimulated DNA synthesis in the S-phase of the cell cycle. Heteroploid cells with a high growth rate possessed a 30-100 times higher alkaline phosphatase activity than in the diploid cells. Under certain conditions alkaline phosphatase may presumably function as a proteinkinase.
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism* / Q000494:pharmacology; D002470:Cell Survival; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D004261:DNA Replication / Q000187:drug effects; D004171:Diploidy; D004622:Embryo, Mammalian; D006801:Humans; D007938:Leukemia / Q000097:blood; D008168:Lung / Q000166:cytology / Q000201:enzymology*; D011003:Ploidies; D013997:Time Factors
chemical_list: D000469:Alkaline Phosphatase
vernacular_title: Aktivnost' shchelochnoĭ fosfatazy v kul'tiviruemykh normal'nykh i transformirovannykh kletkakh cheloveka.