Characterization of synthetic carrier ampholytes by repetitive scanning of the electric field during focusing.
This paper reports the utilization of a potential gradient array detector for monitoring the dynamics of the electric field during isoelectric focusing. Transient and steady state electric field profiles are presented for synthetic carrier ampholyte mixtures with a wide (approximately 3-10) pH range. Two available commercial products (Ampholine and Pharmalyte) and a laboratory synthesized mixture (PEHA ampholytes) are compared. The formation of conductivity gaps and their migration toward the cathode in extended experiments (cathodic drift) can be visualized with this system.
D000665:Ampholyte Mixtures / Q000032:analysis*; D002021:Buffers / Q000032:analysis*; D004563:Electrochemistry; D007525:Isoelectric Focusing
D000665:Ampholyte Mixtures; D002021:Buffers