title: Mutagen-directed attenuation of Rift Valley fever virus as a method for vaccine development.
abstract: Serial mutagenesis with 5-fluorouracil has been employed to derive an attenuated strain of Rift Valley fever virus for use as a live virus vaccine.
mesh_terms: D000818:Animals; D002043:Bunyaviridae / Q000276:immunology*; D002460:Cell Line; D005472:Fluorouracil / Q000494:pharmacology; D006801:Humans; D007928:Lethal Dose 50; D009154:Mutation; D012296:Rift Valley fever virus / Q000235:genetics / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity; D014613:Vaccines, Attenuated; D010948:Viral Plaque Assay; D014765:Viral Vaccines; D014774:Virulence
chemical_list: D014613:Vaccines, Attenuated; D014765:Viral Vaccines; D005472:Fluorouracil