Relationship between intact cell ATP levels and glucocorticoid receptor-binding capacity in the AtT-20 cell.
This study tested the relationship between intact cell ATP levels and glucocorticoid receptor-binding capacity in the AtT-20 mouse pituitary tumor cell. Four treatments were used to alter intact cell ATP levels: incubation with NaF, incubation with 2,4-dinitrophenol, alterations in culture age, and incubation with dexamethasone. Each of these treatments clearly altered glucocorticoid receptor-binding capacity. Their effects on ATP levels, however, were not the same: NaF decreased ATP levels, dinitrophenol decreased ATP levels only transiently, and the last two treatments (increasing culture age and incubation with dexamethasone) produced an inverse relationship between ATP levels and receptor-binding capacity. It is concluded that, at least in the intact AtT-20 cell, receptor-binding capacity is not always directly related to ATP levels.
D019297:2,4-Dinitrophenol; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D010911:Pituitary Neoplasms / Q000378:metabolism*; D011965:Receptors, Glucocorticoid / Q000187:drug effects / Q000378:metabolism*; D012969:Sodium Fluoride / Q000494:pharmacology; D013997:Time Factors
D004140:Dinitrophenols; D011965:Receptors, Glucocorticoid; D003907:Dexamethasone; D000255:Adenosine Triphosphate; D012969:Sodium Fluoride; D019297:2,4-Dinitrophenol