Size analysis of biological membrane vesicles by gel filtration, dynamic light scattering and electron microscopy.
Biological membrane vesicles are analysed in terms of size and size distribution using gel filtration on Sephacryl S-1000, electron microscopy and quasi-elastic light scattering. The agreement between the three methods is satisfactory particularly for homogeneous dispersions. Gel filtration on Sephacryl S-1000 is a quick and convenient method for the routine size analysis of membrane vesicles up to a diameter of about 250 nm.
D002462:Cell Membrane / Q000648:ultrastructure; D002850:Chromatography, Gel; D002856:Chromatophores / Q000648:ultrastructure; D008027:Light; D008566:Membranes / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008871:Microvilli / Q000648:ultrastructure*; D012542:Scattering, Radiation