Ultrastructural analysis of mouse sperm chromatin.
Chromatin organization was studied during the maturation processes in the epididymis and vas deferens; these processes lead to a potentially reversible inactivation of the genome. There are progressive increases in resistance to detergent action and -S-S- bridge reduction in both head membranes and chromatin. In all the sites studied, there was a basic "knobby" chromatin fiber of 110 A diameter. In the caput epididymidis only, in addition to the knobby fibers, there were some smooth fibers, which can be considered as markers of a transient situation in which the stabilization of DNA/protamine interactions has not completely been achieved. In the vas deferens, the knobby fibers, the diameters of which are multiples of that of the basic one, can be converted to single units by increasing the ionic strength.
D000818:Animals; D002843:Chromatin / Q000648:ultrastructure*; D002851:Chromatography, High Pressure Liquid; D004220:Disulfides; D004822:Epididymis / Q000166:cytology; D008297:Male; D051379:Mice; D011092:Polyethylene Glycols; D013079:Sperm Maturation; D013094:Spermatozoa / Q000648:ultrastructure*; D014649:Vas Deferens / Q000166:cytology
D002843:Chromatin; D004220:Disulfides; D011092:Polyethylene Glycols