Antitumor polysaccharides from P. ostreatus (Fr.) Quél.: isolation and structure of a beta-glucan.
We isolated an antitumor glucan (HA beta-glucan) from the neutral polysaccharide fraction (A3) of a hot-water extract of the edible mushroom P. ostreatus (Fr.) Quél. Purification was accomplished by extractions with 20% sodium chloride solution saturated with thymol and by precipitations with ethanol from dimethyl sulfoxide solution. The glucan showed marked antitumor activity at a dose of 0.1 mg/kg. It is a highly branched (1----3)-beta-glucan having an average structure represented by a pentasaccharide segment consisting of one nonreducing terminal, one 3,6-di-O-substituted, and three 3-mono-O-substituted beta-D-glucopyranosyl residues. This structure was confirmed by examining 13C-n.m.r. spectra taken at 75.46 MHz.
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic; D001487:Basidiomycota; D002849:Chromatography, Gas; D003208:Concanavalin A; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008813:Mice, Inbred ICR; D037121:Plant Lectins; D010946:Plants, Medicinal; D011134:Polysaccharides / Q000302:isolation & purification* / Q000627:therapeutic use; D012510:Sarcoma 180 / Q000188:drug therapy
D000972:Antineoplastic Agents, Phytogenic; D037121:Plant Lectins; D011134:Polysaccharides; D003208:Concanavalin A