[Comparative investigations on the precipitation behavior of various protein precipitates in organs and tissues].
The differences of the non-precipitable N-quota in blood plasma, liver, intestine, and muscles after treatment with various precipitants reveal data on the height of protein precipitation in the corresponding body fraction. Organs of hens treated with picric acid (1%), trichloroacetic acid (10%), and sulphosalicylic acid were used for the protein precipitation. Because of contradictory literature data as to the most suitable concentration of sulphosalicylic acid a preliminary determination of the most favourable acid concentration was necessary. The application of a 5% solution of sulphosalicylic acid gave the highest precipitation rate depending on the analyzed organs. In the succession picric acid, trichloroacetic acid, and sulphosalicylic acid nitrogen increases in the soluble supernatant. Furthermore, dependences of the protein precipitation on the kind of the analyzed organs were indicated.
D000818:Animals; D001557:Benzenesulfonates; D001798:Blood Proteins / Q000032:analysis; D002645:Chickens; D005260:Female; D007413:Intestinal Mucosa / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D010851:Picrates; D011506:Proteins / Q000032:analysis*; D012459:Salicylates; D014238:Trichloroacetic Acid
D001557:Benzenesulfonates; D001798:Blood Proteins; D010851:Picrates; D011506:Proteins; D012459:Salicylates; D014238:Trichloroacetic Acid; C005858:picric acid; C003366:sulfosalicylic acid
Vergleichende Untersuchungen zum Präzipitationsverhalten verschiedener Proteinfällungsmittel in Organen und Geweben.