title: An ELISA to detect antibody specific for surface antigens of parasitic nematodes.
abstract: An enzyme immunoassay method is described for the detection of specific immunoglobulins bound to the surface of intact adult Nippostrongylus brasiliensis. Binding of antibody is complete in 1 h and the entire procedure can be completed in less than 4 h. This method has been used to detect the quantity of specific IgG antibodies in sera of rats infected with N. brasiliensis.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D009559:Nippostrongylus / Q000276:immunology*
chemical_list: D000906:Antibodies; D000954:Antigens, Surface