Oligosaccharide composition of an influenza virus hemagglutinin with host-determined binding properties.
We have previously reported that the binding properties of the hemagglutinin (HA) of the WSN-F strain of influenza A are affected by the cells in which the virus is grown (Crecelius, D. M., Deom, C. M., and Schulze, I.T. (1984) Virology 139, 164-177); at 37 degrees C chick embryo fibroblast-grown F virus has a greater affinity for host cells than does the same virus grown in Madin-Darby bovine kidney (MDBK) cells. In an attempt to explain this host-determined property, we have characterized the carbohydrate put onto the viral HA by these two cells. Experiments using tunicamycin indicate that the HA made by MDBK cells contains about 4000 daltons of carbohydrate in excess of that on the HA from chick embryo fibroblast. Serial lectin affinity chromatography of the asparagine-linked oligosaccharides on the HA subunits, HA1 and HA2, detected a number of host-dependent differences in the complex oligosaccharides. Both HA1 and HA2 from MDBK cells contained more highly branched (i.e. tri- and tetraantennary) complex oligosaccharides than did the subunits from chick embryo fibroblasts. In addition, the HA subunits from the two sources differed in the amount of galactose-containing "bisected" complex oligosaccharides and in the presence of certain fucosylated triantennary oligosaccharides. Profiles of the asparagine-linked oligosaccharides from the host cells did not show these differences, indicating that the HA subunit profiles were not necessarily representative of the structures found on the cellular glycoproteins. The data support the conclusion that bulky oligosaccharides on the MDBK-HA subunits of WSN-F reduce the affinity of the virus for cellular receptors.
D000818:Animals; D002417:Cattle; D002460:Cell Line; D002846:Chromatography, Affinity; D006020:Glycopeptides / Q000302:isolation & purification; D006389:Hemagglutinins, Viral / Q000302:isolation & purification*; D009980:Influenza A virus / Q000276:immunology*; D007668:Kidney; D037102:Lectins; D046911:Macromolecular Substances; D009844:Oligosaccharides / Q000032:analysis*
D006020:Glycopeptides; D006389:Hemagglutinins, Viral; D037102:Lectins; D046911:Macromolecular Substances; D009844:Oligosaccharides