The reaction of glutathione with the eye-lens protein gamma-crystallin.
Lens cells contain high concentrations of thiol-rich proteins, gamma-crystallins and reduced glutathione. Solutions of bovine gamma-crystallin react avidly with either reduced or oxidized glutathione to form protein-glutathione mixed disulphides. A method of purification of a gamma-II crystallin-glutathione adduct containing two mixed disulphide groups is described.
D000818:Animals; D002417:Cattle; D003459:Crystallins / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism*; D007525:Isoelectric Focusing; D046911:Macromolecular Substances; D010084:Oxidation-Reduction; D011485:Protein Binding; D013438:Sulfhydryl Compounds / Q000032:analysis
D003459:Crystallins; D046911:Macromolecular Substances; D013438:Sulfhydryl Compounds; D005978:Glutathione