Phenol oxidation product(s), formed by a peroxidase reaction, that bind to DNA.
Phenol oxidation by horseradish peroxidase/H2O2 initially results in p,p'-biphenol and o.o'-biphenol formation and subsequently results in polymer formation. o,o'-Biphenol is the major product formed, but it is rapidly oxidized to the polymer, particularly in the presence of phenol. p,p'-Biphenol is very rapidly oxidized to p,p'-biphenoquinone which can also be involved in polymer formation. Extensive binding of 14C-phenol oxidation products to DNA occurs if the DNA is present in the reaction mixture. However, enzymic hydrolysis of DNA releases the bound polymers. p,p'-Biphenol, however, did not bind to DNA following peroxidase-catalysed oxidation, but o,o'-biphenol readily binds to DNA following peroxidase-catalysed oxidation. Enzymic hydrolysis of the oxidized o,o'-biphenol-bound DNA also resulted in the release of the polymers.
D002250:Carbon Radioisotopes; D004247:DNA / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000494:pharmacology*; D019800:Phenol; D010636:Phenols / Q000378:metabolism*; D011108:Polymers
D002250:Carbon Radioisotopes; D010636:Phenols; D011108:Polymers; D019800:Phenol; D004247:DNA; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D010544:Peroxidases