Identification of a major endogenous substrate for phospholipid/Ca2+-dependent kinase in pancreatic acini as Gc (vitamin D-binding protein).
A major 56 kDa substrate for phospholipid/Ca2+-dependent kinase (C-kinase) in pancreatic acinar cells is physicochemically and immunologically indistinguishable from the vitamin D-binding protein, Gc or group-specific component. Cellular Gc was also phosphorylated in intact cells following treatment with carbachol as a physiological stimulus. These findings indicate the potential usefulness of Gc as a defined substrate for further studies of the biological role of C-kinase activity in pancreatic acini and possibly in other cells.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D010179:Pancreas / Q000201:enzymology*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000032:analysis*; D051381:Rats; D014809:Vitamin D-Binding Protein / Q000302:isolation & purification*
D014809:Vitamin D-Binding Protein; D011493:Protein Kinase C; D002118:Calcium