3[H]-Sulpiride labels mesolimbic non-dopaminergic sites that bind antidepressant drugs.
3[H]-(-)-Sulpiride and 3[H]-spiperone binding was compared in rat amygdala, nucleus accumbens and striatum, using (+/-)-sulpiride to define specific binding. 3[H]-(-)-Sulpiride bound to twice as many sites in amygdala and nucleus accumbens as 3[H]-spiperone. 3[H]-(-)-Sulpiride binding was directed to these additional sites by using 1 microM spiperone to mask dopaminergic binding. The binding of 3[H]-(-)-sulpiride to these sites was high affinity, reversible, Na+-dependent, but not stereospecific. Metoclopramide, tiapride and antidepressant medications, but not other neuroleptics, ADTN, or serotonin displaced 3[H]-(-)-sulpiride binding to these sites. These data suggest that 3[H]-(-)-sulpiride labels mesolimbic sites other than dopamine receptors which may mediate antidepressant effects.
D000679:Amygdala / Q000378:metabolism*; D000818:Animals; D000928:Antidepressive Agents / Q000378:metabolism*; D001665:Binding Sites; D001667:Binding, Competitive; D003342:Corpus Striatum / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008787:Metoclopramide / Q000378:metabolism; D009714:Nucleus Accumbens / Q000378:metabolism*; D051381:Rats; D012686:Septal Nuclei / Q000378:metabolism*; D013134:Spiperone / Q000378:metabolism; D013469:Sulpiride / Q000378:metabolism*; D013976:Tiapamil Hydrochloride / Q000378:metabolism
D000928:Antidepressive Agents; D013134:Spiperone; D013469:Sulpiride; D008787:Metoclopramide; D013976:Tiapamil Hydrochloride