Phosphocitrate inhibition of 45Ca2+ uptake in rat aortic smooth muscle cells in primary culture.
Phosphocitrate inhibits 45Ca2+ uptake by rat aortic smooth muscle cells in primary culture. Unlike the Ca2+ channel blocking effect of diltiazem, the phosphocitrate effect is neither time dependent nor reliant on a depolarized membrane. Phosphocitrate and diltiazem together in maximum inhibitory concentration show an additive effect. The data suggest that different Ca2+ uptake mechanisms are involved. The potential exists for phosphocitrate to have a therapeutic role in atherogenesis through modulating Ca2+ movements in arterial smooth muscle.
D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes / Q000378:metabolism; D002478:Cells, Cultured; D002951:Citrates / Q000378:metabolism / Q000494:pharmacology*; D004110:Diltiazem / Q000494:pharmacology; D004347:Drug Interactions; D007700:Kinetics; D008297:Male; D008564:Membrane Potentials; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D002132:Calcium Radioisotopes; D002951:Citrates; C014148:phosphocitrate; D004110:Diltiazem; D011188:Potassium; D002118:Calcium