Correlation of sister chromatid exchange frequency with mutation induction and cell survival in Chinese hamster ovary cells exposed to N-oxidized arylamines.
In this study the relationships between sister chromatid exchange (SCE) frequency, mutation induction at the hypoxanthineguanine phosphoribosyl transferase locus, and cell survival were established in Chinese hamster ovary cells exposed to one of the three N-oxidized arylamines. The toxicants used were N-hydroxy-2-aminofluorene, N-hydroxy-N'-acetylbenzidine, and 1-nitrosopyrene. Mutation induction as measured by resistance to 6-thioguanine related poorly to reduced cloning ability and SCE frequency. Although SCEs were formed at concentrations of toxicants which produced no measurable reduction in cell survival, a strong correlation was observed between these two biological responses. This relationship was strengthened when the results obtained in this study were combined with those from a previous study which examined the relationship between SCE induction and cell survival in Chinese hamster ovary cells exposed to simple alkylating agents. These results support the contention that a common step is involved in the induction of SCE and cellular toxicity in Chinese hamster ovary cells exposed to certain classes of chemical toxicants.
D000818:Animals; D001560:Benzidines / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D005260:Female; D005449:Fluorenes / Q000633:toxicity*; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D009154:Mutation; D011721:Pyrenes / Q000633:toxicity*; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D001560:Benzidines; D005449:Fluorenes; D011721:Pyrenes; C001368:N-hydroxy-2-aminofluorene; C045050:N-hydroxy-N'-acetylbenzidine; C044707:1-nitrosopyrene; D007041:Hypoxanthine Phosphoribosyltransferase