Polyethylene glycol-facilitated transformation of Bacteroides fragilis with plasmid DNA.
A method for the transformation of Bacteroides fragilis with plasmid DNA was developed by using the clindamycin resistance plasmid pBFTM10 as the source of transforming DNA. The method was technically simple to perform and resulted in an average of 4.2 X 10(3) transformants per microgram of pBFTM10 added. A method for the preparation of frozen competent cells is also described.
D001441:Bacteroides fragilis / Q000235:genetics* / Q000254:growth & development; D004269:DNA, Bacterial; D006863:Hydrogen-Ion Concentration; D010957:Plasmids; D011092:Polyethylene Glycols / Q000494:pharmacology*; D013696:Temperature; D014169:Transformation, Bacterial / Q000187:drug effects*
D004269:DNA, Bacterial; D011092:Polyethylene Glycols