The amino acid sequences of the flavin-peptides of dimethylglycine dehydrogenase and sarcosine dehydrogenase from rat liver mitochondria.
The flavoenzymes dimethylglycine dehydrogenase (EC 1.5.99.2) and sarcosine dehydrogenase (EC 1.5.99.1) contain covalently bound FAD linked via the 8 alpha-position of the isoalloxazine ring to the imidazole N(3) of a histidine residue (Cook, R. J., Misono, K. S., and Wagner, C. (1984) J. Biol. Chem. 259, 12475-12480). The flavin-peptides from tryptic digests of these two enzymes have been isolated and sequenced. Automated sequence analysis showed that the flavin-peptide from dimethylglycine dehydrogenase contained 25 amino acid residues in the following sequence: Ser-Glu-Leu-Thr-Ala-Gly-Ser- Thr-Trp-His(flavin)-Ala-Ala-Gly-Leu-Thr-Thr-Tyr-Phe-His-Pro-Gly-Ile-A sn-Leu-Lys. The sequence determined for the flavin-peptide from sarcosine dehydrogenase contained 14 amino acid residues Leu-Thr-Ser-Gly-Thr-Thr-Trp-His(flavin)-Thr-Ala-Gly-Leu-Gly-Arg.
D000595:Amino Acid Sequence; D000818:Animals; D050893:Dimethylglycine Dehydrogenase; D005182:Flavin-Adenine Dinucleotide; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology*; D024101:Mitochondrial Proteins; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D050892:Sarcosine Dehydrogenase; D013053:Spectrophotometry; D014357:Trypsin / Q000378:metabolism
D024101:Mitochondrial Proteins; D010446:Peptide Fragments; D005182:Flavin-Adenine Dinucleotide; D010089:Oxidoreductases, N-Demethylating; D050892:Sarcosine Dehydrogenase; C494397:Sardh protein, rat; D050893:Dimethylglycine Dehydrogenase; C494400:Dmgdh protein, rat; D014357:Trypsin