Uptake of trimethoprim by renal cortex.
The purpose of this study was to examine the mechanisms involved in the uptake of the urinary antibacterial drug trimethoprim by incubated slices of rat renal cortex. Concentration-dependent studies of the uptake process demonstrated that a saturable component was involved. The results of inhibitor studies as well as the time-course pattern support the conclusion that at least two processes are involved in the uptake of trimethoprim. These include active transport via the organic cation system, accounting for about 40% of the total uptake, and a second component that continues to operate under conditions of inhibited cellular metabolism. Chromatographic examination of post-incubation bathing medium and slice extracts failed to demonstrate renal cortex metabolism of trimethoprim.
D000818:Animals; D001693:Biological Transport, Active; D003080:Cold Temperature; D004140:Dinitrophenols / Q000494:pharmacology; D004734:Energy Metabolism; D005260:Female; D007672:Kidney Cortex / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D009584:Nitrogen / Q000494:pharmacology; D011339:Probenecid / Q000494:pharmacology; D011803:Quinine / Q000494:pharmacology; D011806:Quinolinium Compounds / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D019789:Tetraethylammonium; D013757:Tetraethylammonium Compounds / Q000378:metabolism; D014295:Trimethoprim / Q000378:metabolism*; D014316:Tritium; D010130:p-Aminohippuric Acid / Q000378:metabolism
D004140:Dinitrophenols; D011806:Quinolinium Compounds; D013757:Tetraethylammonium Compounds; D014316:Tritium; C016855:cyanine 863; D019789:Tetraethylammonium; D011803:Quinine; D014295:Trimethoprim; D009584:Nitrogen; D011339:Probenecid; D010130:p-Aminohippuric Acid