Electron microscopic mapping of single-stranded discontinuities in cauliflower mosaic virus DNA by means of the biotin-avidin technique.
Biotin-labeled deoxyuridine triphosphate has been used to label the three natural single-stranded discontinuities of Cauliflower Mosaic Virus DNA. The presence of biotin permitted the direct visualization and mapping of the discontinuities by electron microscopy, using ferritin-labeled avidin.
D001360:Avidin; D001483:Base Sequence; D001710:Biotin; D004277:DNA, Single-Stranded / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D005293:Ferritins; D008854:Microscopy, Electron; D009029:Mosaic Viruses / Q000032:analysis*; D010047:Ovalbumin / Q000031:analogs & derivatives; D010944:Plants
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D001360:Avidin; D001710:Biotin; D010047:Ovalbumin; D005293:Ferritins