Amino acid sequence of heat-stable enterotoxin produced by Vibrio cholerae non-01.
The amino acid sequence of heat-stable enterotoxin, produced by Vibrio cholerae non-01 and isolated from its culture supernatant, was determined by both Edman degradation of native and reductively carboxymethylated enterotoxin and also a combination of fast atom bombardment mass spectrometry and carboxypeptidase Y digestion of native enterotoxin to be as follows: Ile-Asp-Cys-Cys-Glu-Ile-Cys-Cys-Asn-Pro-Ala-Cys-Phe-Gly-Cys-Leu-Asn. This sequence is very similar, but not identical, to those of heat-stable enterotoxins produced by enterotoxigenic Escherichia coli and Yersinia enterocolitica.
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D004768:Enterotoxins; D006358:Hot Temperature; D013058:Mass Spectrometry / Q000379:methods; D014734:Vibrio cholerae / Q000032:analysis*
D004768:Enterotoxins; C037420:stN protein, Vibrio cholerae