Photochemical crosslinking of protein and DNA in chromatin. II. Synthesis and application of psoralen-cystamine-arylazido photocrosslinking reagents.
The synthesis and testing of a new type of nucleic acid-protein photocrosslinking reagent is described. The reagents are composed of a psoralen ligand for nucleic acid photoattachment, which is linked to an azidobenzoyl group, for protein photoattachment. The linker contains a disulfide bridge which can be opened by reduction with mercaptans. The reagents were tested in a chromatin system, where it was found that they induced cleavable crosslinks between the histones and the DNA upon irradiation with long-wavelength ultraviolet light (lambda greater than 300 nm).
D001386:Azides / Q000138:chemical synthesis*; D002843:Chromatin / Q000528:radiation effects*; D003432:Cross-Linking Reagents / Q000138:chemical synthesis*; D003538:Cystamine / Q000138:chemical synthesis*; D004247:DNA / Q000528:radiation effects*; D011564:Furocoumarins / Q000138:chemical synthesis*; D006657:Histones / Q000528:radiation effects; D009698:Nucleoproteins / Q000528:radiation effects*; D010777:Photochemistry; D014466:Ultraviolet Rays
D001386:Azides; D002843:Chromatin; D003432:Cross-Linking Reagents; D011564:Furocoumarins; D006657:Histones; D009698:Nucleoproteins; D004247:DNA; D003538:Cystamine