A spectrophotometric determination of cyanate using reaction with 2-aminobenzoic acid.
A specific method has been devised for the assay of cyanate, based on the reaction with 2-aminobenzoic acid. Cyclization of the product in 6 N HCl results in the formation of 2,4(1H,3H)-quinazolinedione. Cyanate content of the samples can be measured by their absorbances at 310 nm. Alternatively, the second derivatives of the spectra can be recorded; the peak-to-peak height between the first maximum (330 nm) and the first minimum (317 nm) was shown to be proportional to the cyanate content. This method is suitable for the estimation of cyanate in aqueous solutions in the concentration range 0.01 to 2 mM. When added to blood plasma, cyanate could be detected down to 0.1 mM.
D003470:Culture Media / Q000032:analysis; D003485:Cyanates / Q000032:analysis* / Q000097:blood; D006801:Humans; D008832:Microchemistry; D013053:Spectrophotometry / Q000379:methods; D062367:ortho-Aminobenzoates
D003470:Culture Media; D003485:Cyanates; D062367:ortho-Aminobenzoates; C031385:anthranilic acid