Phosphoproteins crosslinked to poly(A) + heterogeneous nuclear RNA after irradiation with ultraviolet light.
Isolated liver nuclei or whole lymph node lymphocytes stimulated with concanavalin A in culture were irradiated with ultraviolet light. The crosslinked structures of poly(A)+ heterogeneous nuclear RNA and protein were purified on oligo(dT)-cellulose after labelling irradiated nuclei in the presence of adenosine 5'-[gamma-32P]triphosphate and analysed by SDS-polyacrylamide gel electrophoresis. The liver and lymphocyte nuclear proteins included about 17-19 species of 35-150 kDa and were shown to produce quite similar electrophoretic band patterns. Two proteins of 110-120 and 40-42 kDa were phosphorylated. Using partial proteolytic digestion the large-size crosslinked phosphoprotein has been identified as the 110 kDa component described previously (Schweiger, A. and Kostka, G. (1984) Biochim. Biophys. Acta 782, 262-268). The 40-42 kDa band was presumably related to the group C species of main proteins associated with heterogeneous nuclear RNA. In crosslinked nuclear structures from rats treated with low doses of alpha-amanitin for 1 h the relative amount of the 110-120 kDa phosphoprotein was reduced while the labelling with [32P]ATP was almost abolished.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D003208:Concanavalin A / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000032:analysis; D008198:Lymph Nodes; D008214:Lymphocytes / Q000032:analysis / Q000187:drug effects; D010750:Phosphoproteins / Q000302:isolation & purification* / Q000528:radiation effects; D010766:Phosphorylation; D011061:Poly A / Q000378:metabolism* / Q000528:radiation effects; D011494:Protein Kinases / Q000378:metabolism; D012332:RNA, Heterogeneous Nuclear / Q000378:metabolism* / Q000528:radiation effects; D051381:Rats; D012261:Ribonucleoproteins / Q000302:isolation & purification* / Q000528:radiation effects; D014466:Ultraviolet Rays
D010750:Phosphoproteins; D012332:RNA, Heterogeneous Nuclear; D012261:Ribonucleoproteins; D003208:Concanavalin A; D011061:Poly A; D011494:Protein Kinases