Ultrastructural localization of chromogranin: a potential marker for the electron microscopical recognition of endocrine cell secretory granules.
Using a monoclonal antibody (LK2H10) directed against human chromogranin, we have been able to localize this soluble glycoprotein to the matrix of secretory granules from a wide variety of endocrine cells. In the gut, enterochromaffin, enteroglucagon, glucose-dependent insulinotropic peptide, gastrin, and neurotensin-containing cells exhibit chromogranin immunoreactivity. In our system, chromogranin-immunoreactive material was restricted to the halo of human pancreatic glucagon-containing secretory granules within A-cells. Chromogranin immunoreactivity was also localized to secretory granules in phaeochromocytomas, gastrinomas, medullary carcinomas of the thyroid and a carotid body tumour (chemodectoma). Chromogranin is proposed as a potential marker for the ultrastructural recognition of endocrine cell secretory granules.
D000293:Adolescent; D000313:Adrenal Medulla / Q000648:ultrastructure*; D000328:Adult; D000911:Antibodies, Monoclonal; D002837:Chromaffin Granules / Q000648:ultrastructure; D002864:Chromogranins / Q000032:analysis*; D003594:Cytoplasmic Granules / Q000648:ultrastructure*; D004064:Digestive System / Q000648:ultrastructure*; D006801:Humans; D007231:Infant, Newborn; D008854:Microscopy, Electron; D009369:Neoplasms / Q000648:ultrastructure*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D010179:Pancreas / Q000254:growth & development / Q000648:ultrastructure*; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D002864:Chromogranins; D009419:Nerve Tissue Proteins