Differential sensitivity of Fanconi anaemia lymphocytes to the clastogenic action of cis-diamminedichloroplatinum (II) and trans-diamminedichloroplatinum (II).
Fanconi anaemia (FA) lymphocytes were tested for their susceptibility to chromosomal breakage by cis-diamminedichloroplatinum (II) [cis-Pt(II)] and its stereoisomer trans-diamminedichloroplatinum (II) [trans-Pt(II)]. Unlike trans-Pt(II), which is a rather inefficient clastogen, cis-Pt(II) is very efficient in inducing chromosomal breakage in FA cells at concentrations that hardly affect control cells. As both cis-Pt(II) and trans-Pt(II) are capable of inducing DNA interstrand crosslinks but only cis-Pt(II) can induce DNA intrastrand crosslinks, this result suggests that FA cells may be specifically sensitive to the intrastrand type of DNA crosslink.
D000293:Adolescent; D000741:Anemia, Aplastic / Q000235:genetics*; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000187:drug effects*; D002945:Cisplatin / Q000494:pharmacology*; D004351:Drug Resistance; D005199:Fanconi Anemia / Q000235:genetics*; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure; D008297:Male; D013237:Stereoisomerism
D002945:Cisplatin