[Bioluminescent method of determining antiprotease activity].
A method for antiprotease activity measurement based on the use of luminous bacteria luciferase as protein substrate of proteases is suggested. Antiprotease is incubated with protease for 1 to 2 min at 30 degrees C and then it is added to the reaction mixture containing luciferase, NADH: FMN-oxidoreductase and their substrates--myristic aldehyde, FMN and NADH. Biofluorescence is measured in a temperature-controlled cuvette for 1 min. The total time of the measurement is 3 min. The method can be applied both in fine biochemical assays and in medical rapid diagnosis.
D006801:Humans; D008156:Luciferases; D008163:Luminescent Measurements; D008722:Methods; D011480:Protease Inhibitors / Q000378:metabolism*
D011480:Protease Inhibitors; D008156:Luciferases
Bioliuminestsentnyĭ sposob opredeleniia aktivnosti antiproteaz.