The effect of i-mu-ts'ao on a partially purified prostaglandin E 9-ketoreductase from swine kidney.
The enzyme system, prostaglandin E 9-ketoreductase, which catalyzes the reduction of prostaglandin E to form prostaglandin F has been partially purified from swine kidney. This NADPH-linked enzyme is studied spectrophotometrically. The KM of this enzyme for prostaglandin E2 was found to be 180 microM. Studies with the partially purified enzyme indicate that prostaglandin E 9-ketoreductase is affected by a Chinese herbal medicine i-mu-ts'ao (leonurus heterophyllus sweet). An increase in the concentration of i-mu-ts'ao aqueous extract may influence the conversion of prostaglandin E2 into prostaglandin F2 alpha. This finding offers a possible explanation for the physiological role of i-mu-ts'ao when it is treated as a medicine.
D000818:Animals; D006911:Hydroxyprostaglandin Dehydrogenases / Q000302:isolation & purification / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D008516:Medicine, Chinese Traditional; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D013379:Substrate Specificity; D013552:Swine
D010936:Plant Extracts; D006911:Hydroxyprostaglandin Dehydrogenases; C035858:prostaglandin-E2 9-reductase