title: Purification and sequence of a novel ovine adrenal medullary peptide and its precursor.
abstract: A 24 amino acid polypeptide that does not originate from (pre)proenkephalin has been isolated from ovine adrenal chromaffin granules. Its sequence is: Arg-Leu-Pro-Gly-Glu-Leu-Arg-Asn-Tyr-Leu-Asp-Tyr-Gly-Glu-Glu-Val-Gly-Glu- Glu-Ala -Ala-Arg-Gly-Val. This peptide is generated from a precursor molecule that has also been purified and partially sequenced. The proteolytic cleavage occurs at a triple Arg site. A search of the available protein sequence data banks shows very little homology to any known protein.
mesh_terms: D000313:Adrenal Medulla / Q000032:analysis*; D000595:Amino Acid Sequence; D000818:Animals; D002837:Chromaffin Granules / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D010455:Peptides / Q000302:isolation & purification*; D011498:Protein Precursors / Q000302:isolation & purification*; D011863:Radioimmunoassay; D012756:Sheep
chemical_list: D010455:Peptides; D011498:Protein Precursors