Effect of sera from various vertebrate species on mouse erythroid progenitor proliferation.
Sera from different vertebrate species were tested for their stimulating activity upon mouse CFU-E growth. Crude sera were either naturally stimulating or toxic. Absorption on mouse blood and spleen cells or decomplementation removes or neutralizes the toxicity, probably mediated through natural anti-mouse antibodies. All but 2 sera tested (ox and sheep) were stimulatory. This stimulating activity was found to be either heat-sensitive or -resistant according to the sera. Upon chromatography on DEAE cellulose, 2 fractions were obtained which were both stimulatory. Whether these two stimulating activities correspond to different molecules or to only 1 absorbed on different serum proteins remains to be determined. These results confirm previous results obtained for mouse and human sera and suggest that this cross-reactive stimulating activity may have an important role in vertebrate erythropoiesis.
D000818:Animals; D001769:Blood; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D005591:Chemical Fractionation; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D008297:Male; D051379:Mice; D013045:Species Specificity; D013154:Spleen / Q000166:cytology; D014714:Vertebrates