Heat-shock treatment lethal for mammalian cells deprived of glucose and glutamine: protection by alpha-keto acids.
Nil and Nilpy hamster cells exposed to temperatures of 44 degrees C to induce the heat-shock proteins survive such exposure for 2 h or more when incubated in Eagle's Minimum Essential Medium with 10% undialyzed fetal calf serum. If D-glucose and L-glutamine are withdrawn from the medium during heat treatment, nearly all the cells are killed by as little as 20 min at 44 degrees C. Several alpha-keto acids, pyruvate, alpha-ketobutyrate, oxaloacetate, and alpha-ketoglutarate, protect cells from the lethal action of the heat treatment in the absence of D-glucose and L-glutamine. L-Glucose and D-glutamine are without effect. Efforts to reverse lethal effects have not been successful.
D000818:Animals; D001769:Blood; D002087:Butyrates / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival; D006224:Cricetinae; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology*; D005947:Glucose / Q000502:physiology*; D005973:Glutamine / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D007651:Keto Acids / Q000494:pharmacology*; D007656:Ketoglutaric Acids / Q000494:pharmacology; D010071:Oxaloacetates / Q000494:pharmacology; D011773:Pyruvates / Q000494:pharmacology; D019289:Pyruvic Acid
D002087:Butyrates; D006360:Heat-Shock Proteins; D007651:Keto Acids; D007656:Ketoglutaric Acids; D010071:Oxaloacetates; D011773:Pyruvates; D005973:Glutamine; C007691:acetylpyruvic acid; C005087:alpha-ketobutyric acid; D019289:Pyruvic Acid; D005947:Glucose