Modification of the kinetic parameters of chicken liver cytoplasmic aspartate aminotransferase by lactate dehydrogenase.
A method for the purification of chicken liver soluble aspartate aminotransferase, lactate dehydrogenase free, is proposed. The preparation, which contained a mixture of the five molecular forms of the enzyme, showed a 120-fold increase in specific activity, with respect to the initial homogenates. Differences in Km(2-oxoglutarate) and saturating concentrations among solutions of purified enzyme and soluble fraction were due to the 2-oxoglutarate reductase activity of lactate dehydrogenase.
D000818:Animals; D001219:Aspartate Aminotransferases / Q000302:isolation & purification / Q000378:metabolism*; D002645:Chickens; D003600:Cytosol / Q000201:enzymology; D005615:Freezing; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*
D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases