Isolation and characterization of a mucin-glycoprotein from rat submandibular glands.
A blood group A+ mucin-glycoprotein was purified from aqueous extracts of rat submandibular glands by sequential chromatography on columns of Sepharose CL-6B and Sephacryl S-300 in urea-containing buffers. Final purification was facilitated by reductive methylation which appeared to release contaminating (hydrophobic) peptides. Homogeneity of the purified mucin was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis at varying concentrations of acrylamide, lectin affinity chromatography, and Western blot analysis. In contrast to previously described preparations, the purified mucin contained only trace amounts of N-acetylglucosamine and aromatic amino acids. In addition, only low levels of basic amino acids were present.
D000017:ABO Blood-Group System; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D037102:Lectins; D008297:Male; D008970:Molecular Weight; D009077:Mucins / Q000302:isolation & purification*; D009844:Oligosaccharides / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence; D013363:Submandibular Gland / Q000032:analysis*
D000017:ABO Blood-Group System; D000596:Amino Acids; D002241:Carbohydrates; D037102:Lectins; D009077:Mucins; D009844:Oligosaccharides