Effects of L-azetidine-2-carboxylic acid on matrix secretion and Golgi structure in fibroblasts and osteoblasts of the mouse.
The effect of a proline analogue, L-azetidine-2-carboxylic acid (LACA), on collagenous matrix secretion by periodontal ligament fibroblasts and alveolar bone osteoblasts was studied by light and electron microscopic radioautography after injection of 3H-glycine; 3H-glycine labeled material accumulated in the cisternae of the rough endoplasmic reticulum and was not secreted for over 4 hours. The Golgi complex of both fibroblasts and osteoblasts showed a marked alteration of its composition after LACA administration. All Golgi saccules and collagen secretion granules disappeared within 2 hours. Flattened Golgi cisternae were still present and appeared to be unaffected by the administration of LACA. These observations indicate that Golgi saccules in collagen-secreting cells are the initial staging areas for the formation of collagen secretory granules and that there is a close correlation between form and function in the Golgi apparatus of collagen-secreting cells.
D000539:Alveolar Process / Q000166:cytology / Q000648:ultrastructure; D000818:Animals; D001345:Autoradiography; D001383:Azetidinecarboxylic Acid / Q000494:pharmacology*; D001385:Azetines / Q000494:pharmacology*; D003094:Collagen / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000648:ultrastructure*; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D010006:Osteoblasts / Q000378:metabolism* / Q000648:ultrastructure; D010513:Periodontal Ligament / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure
D001385:Azetines; D001383:Azetidinecarboxylic Acid; D003094:Collagen