Purification and characterization of the alpha-bungarotoxin binding protein from rat brain.
The alpha-bungarotoxin (BGT) binding protein from rat brain has been purified and its polypeptide chain composition has been examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Polypeptide chains with Mrs of 55,000, 53,500 and 49,000 have been identified as constituents of the protein. The affinity ligand [3H]maleimidobenzyl trimethylammonium bromide ([3H]MBTA), used to identify the ligand binding site on neuromuscular junction acetylcholine receptors (NMJ AChRs), binds to the 55,000 dalton polypeptide chain. Using a technique where ligands are bound to the protein while the protein is immobilized on alpha-cobratoxin-Sepharose 4B, it was established that the brain BGT binding protein, like NMJ AChRs, possesses two binding sites for BGT. These experiments reinforce previous evidence that the brain BGT binding protein is closely related but not identical to NMJ AChRs.
D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D001923:Brain Chemistry / Q000187:drug effects; D002038:Bungarotoxins / Q000378:metabolism*; D002352:Carrier Proteins / Q000302:isolation & purification; D003039:Cobra Neurotoxin Proteins / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D051381:Rats; D011950:Receptors, Cholinergic / Q000302:isolation & purification*; D011978:Receptors, Nicotinic; D064569:alpha7 Nicotinic Acetylcholine Receptor
D002038:Bungarotoxins; D002352:Carrier Proteins; C579165:Chrna7 protein, rat; D003039:Cobra Neurotoxin Proteins; D010455:Peptides; D000644:Quaternary Ammonium Compounds; D011950:Receptors, Cholinergic; D011978:Receptors, Nicotinic; D064569:alpha7 Nicotinic Acetylcholine Receptor; C008313:4-(N-maleimido)benzyltrimethylammonium; C025154:alpha-cobratoxin