Induction of DNA-DNA cross-link formation in human cells by various psoralen derivatives.
Furocoumarin-induced DNA damage, monoadducts, and cross-links were measured in normal human, xeroderma pigmentosum, and Fanconi's anemia cells after exposure to near-UV (356 nm). At similar concentrations and near-UV doses, photoaddition by 8-methoxypsoralen was twice that by angelicin and the substitution of bromodeoxyuridine for thymidine in one strand of DNA did not alter the binding. The rate of cross-linking by 8-methoxypsoralen was twice that of 5-methoxypsoralen. Low frequencies of cross-links were detected from angelicin and 3-carbethoxypsoralen but none were detected from 5-geranoxypsoralen at concentrations up to 25 micrograms/ml and near-UV doses up to 45,000 J/m2.
D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism*; D011564:Furocoumarins / Q000009:adverse effects / Q000378:metabolism*; D006801:Humans; D008970:Molecular Weight; D014316:Tritium
D011564:Furocoumarins; D014316:Tritium; D004247:DNA