Evidence from aequorin for injury of metabolically inhibited myocytes independently of free Ca2+.
The calcium-sensitive photoprotein aequorin has been used to measure cytoplasmic free Ca in single rat ventricle myocytes during metabolic inhibition. Rounding-up of the myocytes, and blebbing of the surface, occurred while free Ca remained at normal resting levels of 1 to 3 X 10(-7)M. Control over free Ca is less susceptible to metabolic blockade than is structural organization.
D000331:Aequorin; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002469:Cell Separation; D003486:Cyanides / Q000494:pharmacology; D006019:Glycolysis / Q000187:drug effects; D008164:Luminescent Proteins; D008845:Microinjections; D009206:Myocardium / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D012508:Sarcolemma / Q000378:metabolism / Q000473:pathology; D013997:Time Factors
D003486:Cyanides; D008164:Luminescent Proteins; D000331:Aequorin; D002118:Calcium