Diabetes in the Bio-Breeding/Worcester rat. Induction and acceleration by spleen cell-conditioned media.
Injections of media conditioned by concanavalin A-activated spleen cells from acutely diabetic rats accelerated the appearance of diabetes in young Bio-Breeding/Worcester (BB/W) rats. Activity was also found in media conditioned by spleen cells from nondiabetic, W-line Wistar Furth and Buffalo rats. Unconditioned media containing mitogen had no activity. Conditioned media also induced diabetes in resistant W-line BB/W rats but not in Wistar Furth rats. A soluble factor may activate a BB lymphocyte population that promotes diabetes.
D000367:Age Factors; D000818:Animals; D001327:Autoimmune Diseases / Q000276:immunology / Q000503:physiopathology*; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media / Q000633:toxicity; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology / Q000503:physiopathology*; D004198:Disease Susceptibility; D005260:Female; D007116:Immunization, Passive; D017710:Lymphocyte Transfusion; D008297:Male; D051381:Rats; D011913:Rats, Inbred BB; D011915:Rats, Inbred BUF; D011919:Rats, Inbred Strains; D011920:Rats, Inbred WF; D013154:Spleen / Q000378:metabolism*
D003470:Culture Media; D003208:Concanavalin A