A gel transfer tank for immunoblotting and its application for analysis of nuclear protein antigens.
The design of a gel transfer tank for immunoblotting is described. It is simple and cheap to make, provides a uniform field and uniform transfer over the whole area of the gel, and can easily be adapted for use with any size of gel. It has been used for transfer of proteins from both sodium dodecyl sulfate-polyacrylamide and isoelectric focusing gels to nitrocellulose membranes and its application to the analysis of nuclear proteins is described.
D000818:Animals; D034961:Antigens, Nuclear; D002467:Cell Nucleus / Q000276:immunology*; D003101:Collodion; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D007120:Immunochemistry / Q000295:instrumentation; D007525:Isoelectric Focusing; D008567:Membranes, Artificial; D051379:Mice; D009363:Neoplasm Proteins / Q000032:analysis / Q000276:immunology; D009698:Nucleoproteins / Q000032:analysis*; D010954:Plasmacytoma / Q000276:immunology; D011506:Proteins / Q000032:analysis* / Q000276:immunology
D034961:Antigens, Nuclear; D008567:Membranes, Artificial; D009363:Neoplasm Proteins; D009698:Nucleoproteins; D011506:Proteins; D003101:Collodion