A convenient S-2-aminoethylation of cysteinyl residues in reduced proteins.
The cysteinyl residues in proteins had been S-2-aminoethylated with ethylenimine quantitatively. However, ethylenimine is now listed as a carcinogen and is no longer commercially available. A method of converting cysteinyl residues to S-2-aminoethyl derivatives quantitatively using 2-bromoethylamine under mild conditions was developed here.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003545:Cysteine / Q000032:analysis*; D005021:Ethylamines; D006868:Hydrolysis; D009113:Muramidase; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D014357:Trypsin
D005021:Ethylamines; D010446:Peptide Fragments; D011506:Proteins; C004504:2-bromoethylamine; D009113:Muramidase; D014357:Trypsin; D003545:Cysteine