Acquisition of glial fibrillary acidic protein-containing fibres by astroglial cells in primary culture is orchestrated by a communicable factor.
In a previous study we discovered that primary cultures initiated from the whole brain of 21-day foetal rats contained astroblasts that concertedly acquired glial fibrillary acidic protein (GFAP) fibres. The mechanism of this burst of cytoskeletal differentiation could not be investigated in these cultures because it occurred too quickly (completed within 2 h). We report that cultures initiated from the region of the third ventricle display an extended burst of GFAP acquisition whose rate could be markedly reduced by medium changing. Temporary medium deprivation or the addition of cytosine arabinoside to the growth medium had no effect. Our findings suggest that an as yet uncharacterised communicable factor is involved in the orchestration of cytoskeletal differentiation in culture. This factor may be responsible for synchronising the appearance of GFAP-positive cells in the periventricular regions of the foetal brain.
D000818:Animals; D001253:Astrocytes / Q000502:physiology*; D001921:Brain / Q000196:embryology*; D002450:Cell Communication; D002454:Cell Differentiation; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000196:embryology; D003561:Cytarabine / Q000494:pharmacology; D003599:Cytoskeleton / Q000502:physiology; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D008636:Mesencephalon / Q000196:embryology; D051381:Rats
D005904:Glial Fibrillary Acidic Protein; D003561:Cytarabine