Structural studies of the rhamnose-glucose polysaccharide antigen from Streptococcus sobrinus B13 and 6715-T2.
The rhamnose-glucose polysaccharide antigens from the cell walls of Streptococcus sobrinus B13 and 6715-T2 (formerly Streptococcus mutans serotypes d and g, respectively) were structurally examined by using gas chromatography-mass spectroscopy. These data confirmed earlier chemical and serological studies suggesting that these polysaccharides had identical structures. The polysaccharides appeared to have a backbone of alternating 1,2- and 1,3-linked rhamnose units. Branching occurred at 1,2,3-linked rhamnose units. Side chains appeared to be composed of 1,2- and 1,6-linked glucose units with glucose as the only terminal carbohydrate.
D000942:Antigens, Bacterial / Q000302:isolation & purification*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000276:immunology; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000032:analysis; D008745:Methylation; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification*; D012210:Rhamnose / Q000032:analysis; D012703:Serotyping; D013045:Species Specificity; D013291:Streptococcus / Q000276:immunology*
D000942:Antigens, Bacterial; D011135:Polysaccharides, Bacterial; D005947:Glucose; D012210:Rhamnose