Quantitative analysis of the composition of mixtures of one-chain and two-chain tissue-type plasminogen activator with a spectrophotometric method.
Tissue-type plasminogen activator (t-PA) exists in two active forms, one-chain t-PA and two-chain t-PA. These two forms differ in their kinetic constants for certain substrates. An assay has been devised to determine the composition of unknown mixtures of both t-PA forms, using the activity measurement on two different substrates. The best results are obtained using plasminogen and less than Glu-Pro-Arg-pNA as substrates.
D006801:Humans; D007700:Kinetics; D009842:Oligopeptides; D010960:Plasminogen Activators / Q000032:analysis* / Q000378:metabolism; D011487:Protein Conformation; D013053:Spectrophotometry; D013379:Substrate Specificity
D009842:Oligopeptides; D010960:Plasminogen Activators