Switch-on of transcriptase function in reovirus: analysis of polypeptide changes using 2-D gels.
Two-dimensional gel electrophoresis (IEF and SDS-PAGE) was used to examine virion polypeptide changes associated with switch-on of transcriptase function in reovirus. Results reveal that switch-on is correlated with altered electrophoretic behavior of a specific minor polypeptide (delta 1) which is present in intermediate subviral particles. A second finding is that each of the molecular weight classes of viral polypeptides exists as a series of subspecies with different isoelectric points. This suggests that extensive posttranslational modification of progeny viral polypeptides occurs during particle morphogenesis. These findings have important theoretical and practical implications.
D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004789:Enzyme Activation; D005786:Gene Expression Regulation; D007526:Isoelectric Point; D007700:Kinetics; D012089:Mammalian orthoreovirus 3 / Q000201:enzymology* / Q000235:genetics; D008970:Molecular Weight; D009024:Morphogenesis; D011499:Protein Processing, Post-Translational; D012087:Reoviridae / Q000201:enzymology*; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases