Nuclear antigens in the HeLa cell cycle.
Antisera to 0.35 M NaCl extracts and residues of S phase HeLa nuclei were reacted with electrophoretically separated proteins from the nuclei or nuclear material of HeLa cells synchronized in G1, S, G2 or M phases of the cell cycle. Quantitative evaluation of the peroxidase-antiperoxidase stained nitrocellulose transfers (Western blots) revealed significant changes in the quantities of nuclear non-histone proteins during the cell cycle. Immunochemical staining of electrophoretically separated nuclear antigens permits their selective detection in minute quantities and in the presence of many additional proteins.
D000941:Antigens / Q000032:analysis; D034961:Antigens, Nuclear; D002453:Cell Cycle; D006367:HeLa Cells / Q000166:cytology / Q000276:immunology*; D006801:Humans; D007399:Interphase; D008677:Metaphase; D009698:Nucleoproteins / Q000032:analysis*
D000941:Antigens; D034961:Antigens, Nuclear; D009698:Nucleoproteins