The effects of soman, in vivo and in vitro, on aldolase activity.
We examined the in vivo and in vitro effects of soman on aldolase activity. Male rats were killed at 4, 6, 9 and 12.5 min after subcutaneous (s.c.) administration of 90 micrograms/kg soman. At 4 min after treatment, aldolase activity was inhibited 55-90% compared to control in cerebral cortex, brainstem, mid-brain and cerebellum; and up to 50% of control in diaphragm and muscle. By 12.5 min, aldolase activity in all areas had returned to control levels except in the diaphragm, which still exhibited 36% inhibition. In vitro activity of rabbit muscle aldolase was not inhibited by 10(-3) M soman. In contrast, diisopropylfluorophosphonate (DFP, 10(-3)-10(-2) M) acted as a competitive inhibitor of aldolase activity in vitro. The results suggest that in vivo, soman effects on aldolase activity are transitory. Any long-lasting effects of soman on aldolase activity may occur only in the periphery, and not in the central nervous system.
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005634:Fructose-Bisphosphate Aldolase / Q000037:antagonists & inhibitors*; D066298:In Vitro Techniques; D007531:Isoflurophate / Q000633:toxicity; D008297:Male; D009132:Muscles / Q000201:enzymology; D011817:Rabbits; D051381:Rats; D012999:Soman / Q000633:toxicity*
D007531:Isoflurophate; D012999:Soman; D005634:Fructose-Bisphosphate Aldolase