title: Effect of age and dietary restriction on protein synthesis by isolated kidney cells.
abstract: The influence of age and food restriction on kidney protein synthesis was studied in Fischer F344 rats. The rate of total protein synthesis by suspensions of kidney cells declined 60% between 4 and 31 months of age. The rate of protein synthesis by kidney cells isolated from 19-month old rats fed a restricted diet (60% of diet consumed by rats fed ad libitum) was 45% higher than the rate of protein synthesis by kidney cells isolated from 19-month old rats fed ad libitum. The excretion of protein in the urine was measured to assess the effect of the age related decline in protein synthesis on kidney function. A dramatic increase in proteinuria was observed with increasing age, and rats fed the restricted diet excreted significantly less protein in the urine than rats fed ad libitum.
mesh_terms: D000375:Aging; D000818:Animals; D002469:Cell Separation; D005110:Extracellular Space / Q000378:metabolism; D005508:Food Deprivation / Q000502:physiology*; D007424:Intracellular Fluid / Q000378:metabolism; D007668:Kidney / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D007700:Kinetics; D008297:Male; D014176:Protein Biosynthesis; D011507:Proteinuria / Q000378:metabolism / Q000503:physiopathology; D051381:Rats; D011916:Rats, Inbred F344; D014633:Valine / Q000378:metabolism
chemical_list: D014633:Valine