[Detection of HBsAg by solid-phase radioimmunological analysis on polyethylene film].
A method of solid-phase radioimmunoassay on polyethylene film has been developed for the detection of HBsAg in human sera. The method records the results autoradiographically, is very capacious, demonstrative and documentary. It has been demonstrated that for at least one month the antibodies previously immobilized on the film retain their capacity to bind with HBsAg that allows timely preparation of the film with immobilized antibody for future analyses. Detection of HBsAg on the film was shown to be specific. The reasons are given for large-scale application of the method in the laboratories not equipped with special radiometric apparatuses.
D000327:Adsorption; D001345:Autoradiography; D005069:Evaluation Studies as Topic; D006509:Hepatitis B / Q000276:immunology; D006510:Hepatitis B Antibodies / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000032:analysis* / Q000302:isolation & purification; D006801:Humans; D011095:Polyethylenes; D011863:Radioimmunoassay / Q000295:instrumentation / Q000379:methods
D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D011095:Polyethylenes
Vyiavlenie HBsAg metodom tverdofazovogo radioimmunologicheskogo analiza na poliétilenovoĭ plenke.