title: Gas chromatographic-mass spectrometric analysis of methylphenidate and p-hydroxymethylphenidate using deuterated internal standards.
abstract: A gas chromatography-mass spectrometry assay is described for the simultaneous determination of threo-dl-methylphenidate and threo-dl-p-hydroxymethylphenidate in plasma and urine using selected ion monitoring of electron impact generated fragments of their pentafluoropropionyl derivatives. The use of recently available deuterated analogues as internal standards improves overall performance relative to previous methods. The practical limit of quantifiable detection of the assay is 0.5 ng/ml for both methylphenidate and p-hydroxymethylphenidate. p-Hydroxymethylphenidate appears to be a significant urinary metabolite of methylphenidate in rats but not in humans.
mesh_terms: D000328:Adult; D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008297:Male; D008774:Methylphenidate / Q000031:analogs & derivatives* / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D012015:Reference Standards
chemical_list: D007202:Indicators and Reagents; D008774:Methylphenidate; C033296:4-hydroxymethylphenidate