Substratum topography and cell traction on sulphuric acid treated bacteriological-grade plastic.
Treatment of bacteriological-grade plastic with concentrated sulphuric acid is a well known technique which increases the wettability of the surface and renders it suitable for eukaryotic cell adhesion. We have noticed that these substrata present a distinctive surface topography in the presence of a serum supplement under normal culture conditions. The adsorbed serum layer is comprised of fine furrows and ridges and the influence of adherent cells on this layer leads to minute tears and distortions in the direction of the corrugations. This provides a novel system for the investigation of cell spreading and locomotion by scanning electron microscopy.
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D006224:Cricetinae; D003470:Culture Media / Q000494:pharmacology; D046508:Culture Techniques / Q000295:instrumentation / Q000379:methods; D005312:Fetal Blood / Q000502:physiology; D005347:Fibroblasts / Q000502:physiology / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D010969:Plastics / Q000032:analysis*; D013464:Sulfuric Acids / Q000494:pharmacology*; D013499:Surface Properties
D003470:Culture Media; D010969:Plastics; D013464:Sulfuric Acids