title: DNA and RNA polymerase activities of nuclei and hypotonic extracts of nuclei isolated from tomato golden mosaic virus infected tobacco leaves.
abstract: Nuclei and hypotonically leached extracts of nuclei prepared from tomato golden mosaic virus (TGMV)-infected Nicotiana benthamiana leaves have been used in in vitro DNA and RNA polymerisation reactions. The synthesis of virus-specific DNA was resistant to aphidicolin, sensitive to N-ethylmaleimide and dideoxy TTP, and stimulated by KC1 and ATP. Variably virion (+) and complementary (-) strand DNA of both the A and B genomic components were synthesised. Virus-specific RNA was synthesised in reactions which were initiated prior to nuclei isolation and leaching. From inhibitor studies and salt requirements RNA synthesis appeared to be catalysed by a DNA-dependent RNA polymerase type II enzyme. Both components of the TGMV genome were transcribed in a bidirectional fashion with a prevalence in some experiments of transcripts derived from DNA component A.
mesh_terms: D002458:Cell Fractionation / Q000379:methods; D002467:Cell Nucleus / Q000201:enzymology* / Q000648:ultrastructure; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D009029:Mosaic Viruses / Q000201:enzymology*; D009994:Osmolar Concentration; D010944:Plants / Q000201:enzymology*; D010947:Plants, Toxic; D014026:Nicotiana / Q000201:enzymology; D014158:Transcription, Genetic
chemical_list: D012321:DNA-Directed RNA Polymerases; D004259:DNA-Directed DNA Polymerase