Flagellar morphology in stumpy-flagella mutants of Chlamydomonas reinhardtii.
Sixteen new mutants of the biflagellate green alga Chlamydomonas reinhardtii with either stumpy-flagella or no flagella at all were examined by electron microscopy. Four of the mutants were found to carry short bulbous flagella containing amorphous electron-dense material which may represent unassembled flagellar protein. Basal bodies of normal ultrastructure were present in all mutants. Dikaryon dominance tests indicated that the stumpy mutations were recessive to wild-type in all cases tested. Stumpy mutations also conferred a measure of detergent resistance to Chlamydomonas, apparently by affecting the detergent-solubility of the flagellar membrane.
D002473:Cell Wall / Q000502:physiology; D002696:Chlamydomonas / Q000187:drug effects / Q000235:genetics / Q000648:ultrastructure*; D003078:Colchicine / Q000494:pharmacology; D005407:Flagella / Q000648:ultrastructure*; D005808:Genes, Recessive; D008854:Microscopy, Electron; D009154:Mutation; D017830:Octoxynol; D010641:Phenotype; D011092:Polyethylene Glycols / Q000494:pharmacology
D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40; D003078:Colchicine