title: Unusual fast myosin isozyme pattern in the lateral gastrocnemius of the chicken.
abstract: The myosin isozyme composition of the lateral gastrocnemius muscle of the chicken leg was investigated during various stages of development utilizing non-denaturing pyrophosphate gel electrophoresis, two-dimensional gel electrophoresis and peptide mapping techniques. An unusual isoform pattern for fast myosin in the lateral gastrocnemius muscle of the adult chicken leg was demonstrated which consisted of a predominance of myosin homodimers and lesser amounts of myosin heterodimer. In addition, a different myosin heavy chain isoform was present in the adult chicken lateral gastrocnemius muscle when compared to other adult fast-twitch muscles. While the adult lateral gastrocnemius muscle contained a different myosin heavy chain isoform from other adult fast-twitch muscles, the embryonic lateral gastrocnemius muscle contained a myosin heavy chain identical to that of the embryonic pectoralis major.
mesh_terms: D000375:Aging; D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D007527:Isoenzymes / Q000302:isolation & purification*; D024510:Muscle Development; D009218:Myosins / Q000032:analysis / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000032:analysis
chemical_list: D007527:Isoenzymes; D010446:Peptide Fragments; D009218:Myosins