Quantification of tumour initiating effect of jute batching oil and its distillates over mouse skin.
In order to identify the tumour initiating constituent(s) of a mineral oil, jute batching oil (JBO), used in the processing of jute fibres, it was fractionally distilled in various boiling range fractions. The latter were then subjected to in vivo assessment of their aryl hydrocarbon hydroxylase (AHH) inducing potential in mouse epidermis. Fractions with almost similar AHH inducing potential were regrouped and studied for their tumour initiating potential over mouse skin following two-stage initiation-promotion protocol and using 12-O-tetradecanoyl phorbol-13-acetate (TPA) as tumour promoter. It was noticed that: (1) JBO as initiator, provoked local development of benign skin tumours over mouse back; (2) fractions of JBO boiling below 335 degrees C and above 399 degrees C accounted for most of the tumour initiating potential of the oil; (3) the histological features of the tumours (i.e. benign papillomas and keratoacanthomas) initiated by these fractions were similar to those developed after being initiated with unfractionated or reconstituted JBO; (4) removal of these fractions from JBO may be attempted which could decontaminate the batch oil from most of its tumorigenic components and make it safer for industrial use.
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000096:biosynthesis; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D051379:Mice; D008899:Mineral Oil / Q000032:analysis / Q000633:toxicity*; D012878:Skin Neoplasms / Q000139:chemically induced*; D013056:Spectrophotometry, Ultraviolet; D013755:Tetradecanoylphorbol Acetate
D008899:Mineral Oil; D001189:Aryl Hydrocarbon Hydroxylases; D013755:Tetradecanoylphorbol Acetate