Chemically defined media for auxotyping of Campylobacter jejuni.
A set of chemically defined media has been developed for the cultivation of Campylobacter jejuni strains of human origin. A minimal medium, a complete medium and 5 different nutrient-deficient media (NDM1-NDM5) are described. Some of the strains investigated required L-methionine(lacking in NDM1), L-cystine and L-cysteine (NDM2), K2HPO4 (NDM 3), KH2PO4 (NDM4) and NAD, thiamine and calcium pantothenate (NDM5). 57.7% of the strains investigated required L-methionine. The strains grew at pH 6.6-7.7. The media described are not suitable for C. intestinalis.
D002168:Campylobacter fetus / Q000145:classification* / Q000254:growth & development / Q000378:metabolism; D003470:Culture Media; D003545:Cysteine / Q000494:pharmacology; D003553:Cystine / Q000494:pharmacology; D005243:Feces / Q000382:microbiology; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008715:Methionine / Q000494:pharmacology; D009243:NAD / Q000494:pharmacology; D010205:Pantothenic Acid / Q000494:pharmacology; D010710:Phosphates / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D017680:Potassium Compounds; D013831:Thiamine / Q000494:pharmacology
D003470:Culture Media; D010710:Phosphates; D017680:Potassium Compounds; D009243:NAD; D010205:Pantothenic Acid; D003553:Cystine; D008715:Methionine; C013216:potassium phosphate; D003545:Cysteine; D011188:Potassium; D013831:Thiamine