title: 25-Hydroxyvitamin D3 3-sulphate is a major circulating form of vitamin D in man.
abstract: 25-Hydroxyvitamin D3 3 beta-sulphate has been identified in human plasma. The compound was isolated by anion-exchange chromatography and following hydrolysis it was characterized by high-performance liquid chromatography and gas chromatography-mass spectrometry. The mean concentration of sulphated 25-hydroxyvitamin D3 in plasma from 60 patients was 16.7 +/- 7.1 ng/ml and the levels often exceeded those of the corresponding free compound. The study also shows that unconjugated 25-hydroxyvitamin D3 is not readily sulphated by man in vivo.
mesh_terms: D002112:Calcifediol / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood*; D002784:Cholesterol / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007774:Lactation; D008099:Liver / Q000378:metabolism; D008895:Milk, Human / Q000032:analysis; D011247:Pregnancy; D012016:Reference Values; D013431:Sulfates / Q000097:blood / Q000378:metabolism
chemical_list: D013431:Sulfates; D002784:Cholesterol; C046949:25-hydroxyvitamin D3 3-sulfate ester; D002112:Calcifediol