On the rotational brownian motion of a bacterial idle motor. II. Theory of fluorescence correlation spectroscopy.
The photon flux autocorrelation function of a fluorescent label attached to a bacterial motor shaft is calculated for the case in which the bacterial motor is considered to be actively but idly rotating. It is shown that even when the fluorescent label has a very short lifetime, fluorescence correlation spectroscopy should provide a useful tool for determining the rate of revolution of the bacterial motor under various solution conditions.
D018407:Bacterial Physiological Phenomena; D002465:Cell Movement; D004058:Diffusion; D008433:Mathematics; D008954:Models, Biological; D055585:Physical Phenomena; D010825:Physics; D012399:Rotation; D013050:Spectrometry, Fluorescence