Synthesis of H-2 antigens by preimplantation mouse embryos.
An enzyme-linked immunosorbent assay (ELISA), which utilized anti-H-2 monoclonal antibody, was used to detect H-2 antigens on preimplantation mouse embryos. All embryonic stages studied, including unfertilized eggs and 1-cell, 2-cell, 8-cell, and blastocyst-stage embryos, showed the presence of H-2 antigens. To prove that the H-2 antigens were not cytophilically adsorbed to the embryos, blastocysts were treated with papain to strip off the H-2 antigens, and then the embryos were further incubated to allow the H-2 antigens to regenerate. After a 3-h incubation time, 60% of the H-2 antigens on the embryos had reappeared, proving that the H-2 antigens were synthesized by the embryos themselves.
D000818:Animals; D004622:Embryo, Mammalian / Q000187:drug effects / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006183:H-2 Antigens / Q000032:analysis*; D051379:Mice; D008815:Mice, Inbred Strains; D010206:Papain / Q000494:pharmacology; D011402:Pronase / Q000494:pharmacology
D006183:H-2 Antigens; D010206:Papain; D011402:Pronase