Solid phase synthesis of gastrin I. Comparison of methods utilizing strong acid for deprotection and cleavage.
A successful synthesis of human gastrin I in 60% overall yield based on the first residue attached to a benzhydrylamine-resin was achieved by the stepwise solidphase method. The synthesis was carried out on a 1% crosslinked polystyrene support, using conventional benzyl-based side chain protecting groups and final deprotection with different acidic protocols. Several improvements in this general approach were applied, including new scavengers, new resin attachment and especially a new technique that allows the strong acid reactions to occur by an SN2 mechanism.
D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D005755:Gastrins / Q000138:chemical synthesis* / Q000302:isolation & purification; D006801:Humans; D007202:Indicators and Reagents; D013056:Spectrophotometry, Ultraviolet
D000596:Amino Acids; D005755:Gastrins; D007202:Indicators and Reagents; C036227:gastrin I