Production of monoclonal antibodies against avidin.
Monoclonal antibodies of the IgG1 subclass were generated against chicken avidin. These antibodies were shown to be as sensitive as polyclonal antiserum in detecting avidin by radioimmunoassay (RIA) and enzyme linked immunosorbent assay (ELISA) methods. Furthermore, the monoclonal antibodies were considerably more specific. Our results with a monoclonal anti-avidin RIA support previous findings that in inflammatory conditions avidin is synthesized also in other organs than the oviduct, although in the liver a major part of the activity detected by polyclonal anti-avidin RIA or biotin-bentonite assay was not due to avidin.
D000818:Animals; D000911:Antibodies, Monoclonal; D001360:Avidin / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D002060:Bursa of Fabricius / Q000378:metabolism; D002645:Chickens; D004054:Diethylstilbestrol / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D007413:Intestinal Mucosa / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000031:analogs & derivatives*; D010057:Oviducts / Q000378:metabolism; D011374:Progesterone / Q000494:pharmacology; D011863:Radioimmunoassay; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D001360:Avidin; D011374:Progesterone; D004054:Diethylstilbestrol; D010047:Ovalbumin