title: Determination of zinc in whole blood, plasma and serum using Zeeman effect flame atomic absorption spectroscopy.
abstract: Methods are presented for the determination of zinc in whole blood, plasma and serum using Zeeman effect flame atomic absorption spectroscopy and a flame microsampling funnel. Whole blood was diluted 1/25 with 0.10 mol/l hydrochloric acid; plasma and serum were diluted 1/5 with deionized water. Concentrations could be read directly from standards prepared in human blood pools. The within-run relative standard deviation (RSD) was 0.50%, 0.82% and 0.61% for whole blood specimens with concentrations of 4 360 micrograms/l, 5 967 micrograms/l and 8 297 micrograms/l, respectively. The within-run RSD was 2.09%, 1.16% and 0.62% for plasma specimens with zinc concentrations of 442 micrograms/l, 976 micrograms/l and 1 731 micrograms/l, respectively. The within-run RSD was 1.18%, 1.22% and 1.02% for serum specimens with zinc concentrations of 492 micrograms/l, 1 023 micrograms/l and 1 533 micrograms/l, respectively. The detection limit was 3.6 micrograms/l.
mesh_terms: D006358:Hot Temperature; D006801:Humans; D008832:Microchemistry; D010949:Plasma / Q000032:analysis; D013054:Spectrophotometry, Atomic / Q000379:methods; D015032:Zinc / Q000097:blood*
chemical_list: D015032:Zinc