Mutagenic activities of dictamnine and gamma-fagarine from dictamni radicis cortex (Rutaceae).
A methanol extract of Dictamni Radicis Cortex exhibited a mutagenic effect on Salmonella typhimurium TA100 and TA98 with S9 mix. Two mutagenic compounds in Dictamni Radicis Cortex were isolated on a Sephadex LH 20 column and silica gel column chromatography and by preparative TLC. These were identified as dictamnine and gamma-fagarine by UV, EI-Mass, 1H-NMR. Dictamnine and gamma-fagarine were mutagenic in strain TA100 and TA98 with S9 mix. The dose-response curves were linear in the range 10-40 micrograms. Dictamnine and gamma-fagarine had specific activities (His+/microgram) of about 50-70 revertant colonies in strain TA100, while in strain TA98 there were about 30-50 revertant colonies.
D000470:Alkaloids / Q000633:toxicity*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D008516:Medicine, Chinese Traditional; D008518:Medicine, East Asian Traditional; D009152:Mutagenicity Tests; D009153:Mutagens / Q000302:isolation & purification*; D010936:Plant Extracts / Q000633:toxicity; D010946:Plants, Medicinal / Q000032:analysis*; D011804:Quinolines / Q000633:toxicity*; D051381:Rats
D000470:Alkaloids; D009153:Mutagens; D010936:Plant Extracts; D011804:Quinolines; C049193:gamma-fagarine; C026398:dictamnine