Effect of depletion of epidermal dendritic cells on the induction of contact sensitivity in the guinea-pig.
Guinea pig skin was depleted of Langerhans cells (LC) as assessed by ATPase and Ia staining using several techniques. The LCs were depleted either by tape-stripping or exposure of the animals to UV-B or UV-C radiation. Guinea-pigs were sensitized to 2,4-dinitrochlorobenzene (DNCB) by application of the sensitizer to the epidermis depleted of LC. Minimally suppressed contact reactions were found in animals exposed to both wavelengths of radiation, but this was shown to be a systemic rather than a local effect. Tape-stripping did not alter the degree of contact sensitivity when guinea-pigs were sensitized with a large dose of DNCB. When a non-sensitizing dose of DNCB was applied to the ear depleted of LC by tape-stripping, contact sensitivity resulted. Although the depletion of LCs was 97% following UV-B, 93% with UV-C and 78% after tape-stripping, at no time were LCs completely absent from the epidermis.
D000818:Animals; D002452:Cell Count; D002469:Cell Separation / Q000379:methods; D003877:Dermatitis, Contact / Q000209:etiology / Q000276:immunology / Q000473:pathology*; D004137:Dinitrochlorobenzene / Q000276:immunology; D004817:Epidermis / Q000473:pathology* / Q000528:radiation effects; D005260:Female; D006168:Guinea Pigs; D007108:Immune Tolerance; D007801:Langerhans Cells / Q000502:physiology*; D014466:Ultraviolet Rays
D004137:Dinitrochlorobenzene