Comparative analysis of rat uterine estrogen-induced creatine kinase with monoclonal antibodies.
Monoclonal antibodies were generated against rat uterine estrogen-induced protein--creatine kinase (CK-EIP)--and two (MAb-28 and MAb-78) were studied. These antibodies were IgM but differed in their complementary antigenic determinants both of which were detectable on denatured but not on native CK-EIP. MAb-28 reacted with other CK-BBs but not with CK-MMs whereas MAb-78 reacted with both types of CKs. A measurement of antigenicity with the monoclonal antibodies under calibrated conditions showed differences among the CKs, notably between CK-BB from rat brain and CK-EIP when both were probed with MAb-28. The antigenicity of CK-BB (rat brain) was significantly lower than that of CK-EIP, indicating that the former either expresses less copies of the determinant recognized by MAb-28 than CK-EIP does, or possesses a determinant which interacts with the antibody with lower affinity. The monoclonal antibodies should help elucidate structure-function relationships in CK-BB and CK-EIP molecules, their anatomic distribution and their physiologic, pathologic and experimental variations in relation to gene expression induced by sex hormones.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001921:Brain / Q000201:enzymology; D003402:Creatine Kinase / Q000096:biosynthesis / Q000276:immunology*; D004790:Enzyme Induction / Q000187:drug effects; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D011489:Protein Denaturation; D051381:Rats; D013329:Structure-Activity Relationship; D014599:Uterus / Q000201:enzymology*
D000911:Antibodies, Monoclonal; D004967:Estrogens; D003402:Creatine Kinase