Stable phenotypic expression by chick chondroblasts in long-term suspension cultures as determined by proteoglycan analysis.
The influence of cell shape on phenotypic expression was studied in chick vertebral chondroblasts maintained for several weeks in suspension culture. To monitor phenotypic expression, synthesis of proteoglycans was studied in cultures of freshly-isolated 1-day-old chondroblasts and 1-to-6-week-old chondroblasts. The rate of proteoglycan synthesis was virtually identical in 1-week or older chondroblasts; however, this rate was 3- to 5-fold higher than in 1-day-old cells. When compared to the latter cells, the various populations of older chondroblasts synthesized monomers of the major cartilage proteoglycan (KS: CS-PG) of slightly lower molecular size and a lower level of unsubstituted N-acetylgalactosamine residues on their core protein but with similar chondroitin sulfate chains and levels of O-linked oligosaccharides. At no time of culture were changes in the proportions of the major vs the minor cartilage proteoglycans detected. The results suggest that in contrast to epithelioid chondroblasts in standard monolayer cultures studied previously, the round floating chondroblasts express very stable biosynthetic properties for a prolonged time in suspension. The distinct biosynthetic properties of 1-day-old chondroblasts are discussed in terms of an initial, transitory response to the culture condition and in relation to regulatory mechanisms for proteoglycan elaboration.
D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D002642:Chick Embryo; D005786:Gene Expression Regulation; D006025:Glycosaminoglycans / Q000032:analysis; D007700:Kinetics; D009844:Oligosaccharides / Q000032:analysis; D010641:Phenotype; D011509:Proteoglycans / Q000096:biosynthesis*
D006025:Glycosaminoglycans; D009844:Oligosaccharides; D011509:Proteoglycans