title: Masticatory lubrication. The role of carbohydrate in the lubricating property of a salivary glycoprotein-albumin complex.
abstract: We report for the first time a masticatory-lubrication assay system to assess the lubricating properties of salivary constituents. The lubricating ability of the proline-rich glycoprotein (PRG) of parotid saliva was enhanced by human serum albumin. The interactive effect of albumin was abolished by chemically deglycosylating the glycoprotein. Fluorescence spectroscopy with a hydrophobic probe verified the existence of a PRG-albumin complex and demonstrated that deglycosylation of the PRG altered the nature of its interaction with albumin.
mesh_terms: D000328:Adult; D000596:Amino Acids / Q000032:analysis; D050260:Carbohydrate Metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D046911:Macromolecular Substances; D008297:Male; D008409:Mastication; D010455:Peptides / Q000378:metabolism*; D055232:Proline-Rich Protein Domains; D011485:Protein Binding; D012471:Salivary Proteins and Peptides / Q000378:metabolism*; D012709:Serum Albumin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D000596:Amino Acids; D046911:Macromolecular Substances; D010455:Peptides; D012471:Salivary Proteins and Peptides; D012709:Serum Albumin