Human secondary immune responses induced by influenza in a serum-free tissue culture system.
Peripheral blood lymphocytes are capable of producing antibody in response to antigenic stimulation by a recall antigen, influenza, during culture for 10 days in medium consisting of 1 part Ham's F-12 and 1 part Iscove's modified Dulbecco's medium supplemented with sodium bicarbonate, bovine crystalline insulin, human transferrin, 2-mercaptoethanol, progesterone, and bovine serum albumin. Anti-influenza antibody levels in the supernatants were determined by enzyme-linked immunosorbent assay. Optimal conditions for production of anti-influenza antibody in this serum-free medium were: influenza concentration, 0.032-0.125 HAU/ml; day of harvest, 10; and cell concentration, 3.0-4.0 X 10(5) cells in 200 microliter of medium per well. Use of serum-free medium will allow examination of the effects of various additives to tissue culture without concern for unknown factors or potential interaction with serum.
D000914:Antibodies, Viral / Q000096:biosynthesis*; D000921:Antibody-Producing Cells / Q000187:drug effects / Q000276:immunology*; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis*; D009980:Influenza A virus / Q000276:immunology*; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D000914:Antibodies, Viral; D003470:Culture Media; D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine