title: A futile cycle in erythrocyte glycolysis.
abstract: The storage lesion which limits the shelf life of human blood in blood banking is associated with a metabolic loss of 2,3-diphosphoglycerate and ATP. This metabolic loss is driven by intracellular ATPase which are usually considered to include the ion pumps and the reactions which maintain the discoid shape of the human erythrocyte. Under the acidic conditions of blood storage, the energy-yielding reactions of the glycolytic pathway are restricted at the hexokinase and phosphofructokinase steps. We show here that under such circumstances the enzyme of the diphosphoglycerate shunt, diphosphoglycerate mutase/phosphatase and the glycolytic enzyme phosphoglycerate kinase can form a futile cycle with ATPase activity. This ATPase activity responds to 2-phosphoglycolate which is known to activate both diphosphoglycerate mutase and diphosphoglycerate phosphatase reactions. When the enzymes of the futile cycle are combined with the enzymes of the lower glycolytic pathway in a reconstitution experiment designed to represent conditions within the stored erythrocyte, the futile cycle does provide an ATPase activity which results in the metabolic loss of 2,3-diphosphoglycerate. An isotope incorporation experiment demonstrates that the futile cycle is active in glucose-depleted erythrocytes.
mesh_terms: D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate / Q000378:metabolism; D004163:Diphosphoglyceric Acids / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D006019:Glycolysis; D006801:Humans; D009243:NAD / Q000378:metabolism; D010735:Phosphoglycerate Kinase / Q000378:metabolism
chemical_list: D004163:Diphosphoglyceric Acids; D009243:NAD; D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate; D010735:Phosphoglycerate Kinase