Phospholipids and cholesterol in the liver mitochondrial fraction in mice with transplantable leukemia P 388.
BDF1 hybrid mice, were given 10(4) lymphatic leukemia cells P 388 intraperitoneally. They were killed on 5th day and 11th day of the experiment. In the plasma the level of cholesterol was determined. Inner organs, i.e. liver, spleen, lymph nodes and thymus were verified histopathologically. Mitochondrial fraction lipids were extracted from liver and separated by thin layer chromatography. It was proved that the amount of mitochondrial fraction lipids of leukemic mice was considerably higher than in healthy mice and it increased together with the process of the neoplasma. In the group of leukemic animals the level of phosphatidylcholine, phosphatidylethanolamine, cholesterol and sphingomyelin increased in comparison with healthy mice. The results concerning phospholipids and cholesterol level in leukemic animals were interpreted in the aspect of the interaction of these compounds with vitamin E, polyunsaturated fatty acids and free radicals.
D000818:Animals; D002784:Cholesterol / Q000032:analysis / Q000097:blood; D005260:Female; D007425:Intracellular Membranes / Q000032:analysis; D007941:Leukemia P388 / Q000378:metabolism*; D007942:Leukemia, Experimental / Q000378:metabolism*; D008563:Membrane Lipids / Q000032:analysis; D051379:Mice; D008930:Mitochondria, Liver / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis
D008563:Membrane Lipids; D010743:Phospholipids; D002784:Cholesterol