title: Changes of plasma membrane proteins during prespore differentiation in Dictyostelium discoideum.
abstract: By the use of a shake culture system, we have previously shown (Oyama, M., Okamoto, K., & Takeuchi, I. (1982) J. Cell Sci. 56, 223-232) that both cAMP and cAMP-dependent cell contact are required for prespore differentiation in Dictyostelium discoideum. The present study was undertaken to examine changes of the plasma membrane proteins during prespore differentiation in the shake culture system. Rabbit antibodies prepared against the plasma membrane fraction of the differentiated cells inhibited the reaggregation of the differentiated cells but not that of aggregation-competent cells. This result indicates that new contact sites are formed in the differentiated cells. By the combined use of the antibody-conjugated immuno-adsorbent with sodium dodecyl sulfate-polyacrylamide gel electrophoresis, changes of membrane proteins were analyzed with the cells incubated under various conditions. Three proteins were found to be present specifically in the differentiated cells only in the presence of cAMP, one of which (105K protein) appeared when cells became adhesive, but before prespore specific proteins were detected. Two others (80K and 58K proteins) appeared during prespore differentiation after cells formed agglomerates.
mesh_terms: D000918:Antibody Specificity; D002448:Cell Adhesion; D002454:Cell Differentiation; D002462:Cell Membrane / Q000502:physiology*; D004023:Dictyostelium / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D008565:Membrane Proteins / Q000276:immunology / Q000502:physiology*; D013172:Spores, Fungal / Q000166:cytology
chemical_list: D008565:Membrane Proteins