Genotoxicity of carcinogenic N-nitrosopropylamine derivatives in the hepatocyte primary culture/DNA-repair test.
The genotoxicity of N-nitrosodipropylamine, 8 of its oxidized derivatives and N-nitroso-2,6-dimethylmorpholine was examined in the hepatocyte primary culture (HPC)/DNA repair test. Nine N-nitrosamines which are known to be carcinogenic and mutagenic were clearly positive in the HPC/DNA-repair test. N-Nitroso(2,3-dihydroxypropyl) (2-hydroxypropyl)amine did not elicit DNA repair, but showed a borderline mutagenic response in the Salmonella/microsome test. Thus, the HPC/DNA-repair test displays a comparable capacity to the bacterial mutagenesis test for detecting the genotoxic effects of this class of carcinogens.
D000818:Animals; D002478:Cells, Cultured; D004260:DNA Repair / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D008099:Liver / Q000187:drug effects*; D008297:Male; D008861:Microsomes / Q000378:metabolism; D009152:Mutagenicity Tests; D009602:Nitrosamines / Q000633:toxicity*; D051381:Rats; D013329:Structure-Activity Relationship
D009602:Nitrosamines; C013161:N-nitroso(di-n-propyl)amine