Ca2+-independent secretion is dependent on cytoplasmic ATP in human platelets.
The secretion evoked in human platelets by physiological agonists is an energy-requiring process that depends on the metabolic ATP pool. Diacylglycerol, phorbol ester and collagen evoke a secretory response, which is not associated with an increase of cell Ca2+ levels, and is attributed to activation of protein kinase C [(1983) Nature 305, 317-319]. The secretion evoked by these agonists decreased along with cytoplasmic ATP depletion in the same way that the thrombin-induced secretion did. The secretory response was restored by raising again the cytoplasmic ATP levels. These results support the idea that the secretory response takes place by the physiological ATP-dependent mechanisms rather than by membrane perturbations in these instances.
D000255:Adenosine Triphosphate / Q000097:blood*; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D002118:Calcium / Q000097:blood*; D003094:Collagen / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism; D003847:Deoxyglucose / Q000494:pharmacology; D004075:Diglycerides / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D010750:Phosphoproteins / Q000097:blood; D010974:Platelet Aggregation; D011493:Protein Kinase C / Q000097:blood; D012966:Sodium Cyanide / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013917:Thrombin / Q000494:pharmacology
D004075:Diglycerides; D010750:Phosphoproteins; D000255:Adenosine Triphosphate; D003094:Collagen; D003847:Deoxyglucose; D011493:Protein Kinase C; D013917:Thrombin; D013755:Tetradecanoylphorbol Acetate; D012966:Sodium Cyanide; D002118:Calcium