Purification of the pepsinogen A isozymogens by means of high resolution ion-exchange chromatography. Evidence for post-translational modifications.
Total human pepsinogen (PG) was isolated from gastric fundic mucosa and PGA (formerly called PGI) from urine, using standard ion-exchange and gel filtration techniques. Gastric PGA was separated from PGC (formerly called PGII) either by immunoaffinity or high resolution ion-exchange chromatography (fast protein liquid chromatography, Pharmacia, Uppsala, Sweden). The individual PGA isozymogens 2, 3, 4 and 5 could be isolated to homogeneity with the aid of the same ion-exchanger. Evidence was obtained for the existence of secondary modifications of the PGA fractions 3, 4 and 5, electrophoretically overlapping the primary (genetic) isozymogens.
D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005753:Gastric Mucosa / Q000201:enzymology; D006801:Humans; D007527:Isoenzymes / Q000302:isolation & purification*; D010435:Pepsinogens / Q000235:genetics / Q000302:isolation & purification*; D014176:Protein Biosynthesis
D007527:Isoenzymes; D010435:Pepsinogens