Identification and quantitation of sulfamethazine metabolites by liquid chromatography and gas chromatography-mass spectrometry.
Methods for the identification and quantitation of carbon-14 labeled sulfamethazine [4-amino-N-(4,6-dimethyl-2-pyrimidinyl)benzenesulfonamide], N4-acetylsulfamethazine, the N4-glucose conjugate of sulfamethazine, and desaminosulfamethazine in swine tissue are described. Tissues are ground and extracted with methanol, and the 14C-labeled compounds are purified by XAD-2 column chromatography and C-18 reverse phase liquid chromatography (LC) and the 14C-labeled compounds are then methylated and identified by gas chromatography-mass spectrometry analysis. Quantitation is accomplished by measuring the amount of 14C-activity that cochromatographs (C-18 reverse phase LC) with reference compounds.
D000818:Animals; D001711:Biotransformation; D002853:Chromatography, Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006868:Hydrolysis; D008745:Methylation; D013418:Sulfamethazine / Q000032:analysis* / Q000652:urine; D013552:Swine
D013418:Sulfamethazine