title: Structure of a novel phosphoglycolipid from Deinococcus radiodurans.
abstract: The chemical structure of a major phosphoglycolipid from Deinococcus radiodurans has been shown to be 2'-O-(1,2-diacyl-sn-glycero-3-phospho)-3'-O-(alpha-galactosyl)-N-D-gl yceroyl alkylamine. By infrared spectroscopy, the lipid was shown to contain both carbonyl ester and amide linkages. Chemical analysis demonstrated a molar ratio of fatty acid, carbohydrate, and phosphorus of 2:1:1. The lipid was shown to contain an sn-3-phosphatidic acid backbone by digestion with phospholipase A2. Phosphodiester bond cleavage of the lipid with hydrofluoric acid liberated a component which contained galactose, glyceric acid, and alkylamines. Using NMR and permethylation/hydrolysis procedures, galactose was shown to be linked alpha-glycosidically to the 3-O-position of glyceric acid.
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D008401:Gas Chromatography-Mass Spectrometry; D006017:Glycolipids / Q000302:isolation & purification*; D006094:Gram-Positive Bacteria / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D010743:Phospholipids / Q000302:isolation & purification*; D013055:Spectrophotometry, Infrared
chemical_list: D006017:Glycolipids; D010743:Phospholipids