title: Placental alkaline phosphatase types and subtypes determined by agarose gel electrophoresis and separator isoelectric focusing.
abstract: Two techniques for phenotyping the human placental alkaline phosphatase system were developed: high-voltage agarose-gel electrophoresis and thin-layer separator isoelectric focusing on agarose. These methods enabled a more rapid and sensitive phenotyping of all common phenotypes than the traditionally employed starch-gel electrophoresis. An extended polymorphism of placental alkaline phosphatase was revealed by isoelectric focusing. The existence of two suballeles of Pl1 allele and two suballeles of Pl2 allele was postulated.
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis / Q000235:genetics*; D010641:Phenotype; D010920:Placenta / Q000201:enzymology*; D011110:Polymorphism, Genetic; D011247:Pregnancy
chemical_list: D007527:Isoenzymes; D000469:Alkaline Phosphatase