Capillary gas chromatographic analysis of volatile and non-volatile organic acids from biological samples as the t-butyldimethylsilyl derivatives.
A quantitative procedure for the analysis of volatile organic acids and lactic acid in silage is described. The samples were extracted with diethyl ether, derivatized by t-butyldimethylsilylation, and then separated by capillary gas chromatography. The same procedure was useful for the identification by gas chromatography/mass spectrometry of organic acids in samples such as the metabolic fermentation products of anaerobic bacteria.
D000143:Acids / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D007202:Indicators and Reagents; D017646:Organosilicon Compounds; D012825:Silicon
D000143:Acids; D007202:Indicators and Reagents; D017646:Organosilicon Compounds; C009660:t-butyldimethylsilyl compounds; D012825:Silicon