A radioimmunoassay for the measurement of aminopeptidase (microsomal) in human serum.
A radioimmunoassay for the measurement of aminopeptidase (microsomal) (AP) in human serum was developed by using antiserum to human kidney AP. AP purified from kidney and AP present in normal serum and in serum from a patient with obstructive jaundice gave parallel logit-log transformation lines, suggesting immunological identity. The mean concentration of AP in normal serum (n = 104) was 1.33 +/- 0.30 (mean +/- SD) micrograms/ml. Men had significantly higher serum AP levels (1.41 +/- 0.30 micrograms/ml) (p less than 0.005) than women (1.24 +/- 0.28 micrograms/ml). Serum AP levels of patients with hepatoma (2.26 +/- 0.87 micrograms/ml) and cancer of the pancreas or the biliary tract (2.90 +/- 0.67 micrograms/ml) were significantly higher (p less than 0.005) than those of normal subjects. Patients with acute and chronic hepatitis (2.06 +/- 0.66 micrograms/ml) also had significantly higher serum AP levels (p less than 0.005) than normal subjects. In pregnant women, however, the increase in AP activity without the increase in AP concentration showed that the increased AP activity was due to an enzyme other than AP. The enzyme levels and activities in normal serum as well as in patients' sera were significantly correlated (normal, r = 0.77; patients, r = 0.95). Based on the specific activity of AP purified from human plasma, the enzyme activity splitting L-alanyl-beta-naphthylamide is due almost completely to AP in normal subjects and in patients with hepatobiliary diseases.
D000328:Adult; D000368:Aged; D000626:Aminopeptidases / Q000097:blood*; D005260:Female; D006505:Hepatitis / Q000201:enzymology; D006801:Humans; D007938:Leukemia / Q000201:enzymology; D008297:Male; D008861:Microsomes / Q000201:enzymology; D008875:Middle Aged; D009369:Neoplasms / Q000201:enzymology; D011247:Pregnancy; D011863:Radioimmunoassay; D012044:Regression Analysis; D012737:Sex Factors
D000626:Aminopeptidases