Transferrin subtyping of human bloodstains.
A method was described for subtyping transferrin derived from human bloodstains. Bloodstain cuttings were extracted in 0.5% ferrous ammonium sulfate. The extracts were subjected to ultrathin-layer polyacrylamide gel isoelectric focusing. After isoelectric focusing, transferrin was detected by silver staining. This method permitted the successful typing of Tf in 6-month-old blood stains maintained at -20 degrees C and room temperature and 3-month-old bloodstains maintained at 37 degrees C.
D001788:Blood Grouping and Crossmatching / Q000379:methods*; D001801:Blood Stains; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007525:Isoelectric Focusing; D010641:Phenotype; D014168:Transferrin / Q000032:analysis* / Q000235:genetics
D014168:Transferrin