Effects of dolichol and dolichyl phosphate on in vitro differentiation of hematopoietic progenitors.
The exogenous addition of dolichyl phosphate (Dol-P), an active form of dolichol (Dol) that carries oligosaccharide chains for protein-N-glycosylation, significantly enhanced colony formation of mouse bone marrow hematopoietic progenitors (CFU-e, BFU-e, and CFU-gm) was stimulated by erythropoietin (Epo) and colony-stimulating factor (CSF), but Dol enhanced colony formation of CFU-e only. The effects of Dol or Dol-P on these hematopoietic progenitors were fully dependent on stimulation by Epo or CSF. Other mevalonate-metabolites, such as cholesterol, coenzyme Q10, and isopentenyladenine, had no effect on hematopoietic progenitors. These studies suggest that exogenous Dol-P enhances the frequency of differentiation of hematopoietic progenitors stimulated by Epo or CSF, and there may be a diversity in cellular response of these progenitors to Dol.
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002784:Cholesterol / Q000494:pharmacology; D004224:Diterpenes / Q000494:pharmacology*; D004288:Dolichol Phosphates / Q000494:pharmacology*; D004286:Dolichols / Q000494:pharmacology*; D004920:Erythropoiesis / Q000187:drug effects; D004921:Erythropoietin / Q000494:pharmacology; D005260:Female; D006098:Granulocytes / Q000166:cytology; D006410:Hematopoiesis / Q000187:drug effects*; D007541:Isopentenyladenosine; D008264:Macrophages / Q000166:cytology; D051379:Mice; D011106:Polyisoprenyl Phosphates / Q000494:pharmacology*; D014451:Ubiquinone / Q000494:pharmacology
D004224:Diterpenes; D004288:Dolichol Phosphates; D004286:Dolichols; D011106:Polyisoprenyl Phosphates; D004921:Erythropoietin; D014451:Ubiquinone; C001478:N(6)-(delta(2)-isopentenyl)adenine; D007541:Isopentenyladenosine; D002784:Cholesterol; D000225:Adenine