Stimulation of myogenesis by 2,2'-thiodiethanol in suboptimal tissue culture conditions.
It is shown that 2,2'-thiodiethanol, a product of yperite hydrolysis, strongly stimulates differentiation of chick embryo myogenic cells. In its presence myoblasts fused, yielding myotubes with the same efficiency in standard media for chick embryo fibroblast-like cell culture (containing 4% bovine serum and 1% chick serum) as in media specially designed to promote myoblast fusion (containing 10% horse serum and 5% chick serum). What is more, the myofibres formed in the presence of 0.1% 2,2'-thiodiethanol morphologically resembled more closely myofibres formed in vivo than those formed in the presence of horse serum.
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D003402:Creatine Kinase / Q000378:metabolism; D003470:Culture Media; D006133:Growth Substances / Q000494:pharmacology*; D007700:Kinetics; D024510:Muscle Development; D009132:Muscles / Q000166:cytology* / Q000187:drug effects; D013438:Sulfhydryl Compounds / Q000494:pharmacology*; D013997:Time Factors
D003470:Culture Media; D006133:Growth Substances; D013438:Sulfhydryl Compounds; C047519:2,2'-thiodiethanol; D003402:Creatine Kinase