[Damage to the supramolecular DNA complex in the mechanism of action of antitumor drugs].
The report deals with damaging effects on the structure of a supramolecular DNA complex of leukemic cells in P388 mice, susceptible to most antitumor preparations, following treatment with therapeutic doses of sarcolysin, methylnitrosourea, actinomycin D and adriamycin. Capillary elastoviscosimetry and nucleoid sedimentation procedure showed that damage to supramolecular DNA complex structure became apparent as quickly as 15-60 min after injection of the said preparations and persisted for several days. The said damage consisted in DNA's filaments rupture and alterations in higher-order chromatin structures. The role of conformational changes of supramolecular DNA complex in the course of the cytotoxic action of antitumor agents is discussed.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D004273:DNA, Neoplasm / Q000032:analysis*; D004277:DNA, Single-Stranded / Q000032:analysis; D004278:DNA, Superhelical / Q000032:analysis*; D007941:Leukemia P388 / Q000188:drug therapy; D046911:Macromolecular Substances; D051379:Mice; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D014783:Viscosity
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded; D004278:DNA, Superhelical; D046911:Macromolecular Substances
Povrezhdenie nadmolekuliarnogo kompleksa DNK v mekhanizme deĭstviia protivoopukholevykh preparatov.