Distinction of three types of D-glucose transport systems in animal cells.
Immunoblotting of plasma membrane fractions from rat kidney cortex with antibody to human erythrocyte glucose transporter showed a single major cross-reacting material of 48K in basolateral membrane fractions possessing a facilitated diffusion system for D-glucose, but not in brush border membrane fractions which have a Na-dependent active transport system. Cytochalasin B inhibited D-glucose uptake in basolateral membrane vesicles but not in brush border vesicles. Cross-reacting materials of 44-55K were detected in several animal cells exhibiting facilitated diffusion systems, including a hormone dependent system. These results indicate molecular difference between glucose transporters of facilitated diffusion systems and active transport systems.
D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D003429:Cross Reactions; D003571:Cytochalasin B / Q000494:pharmacology; D004058:Diffusion / Q000187:drug effects; D004912:Erythrocytes / Q000032:analysis; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007672:Kidney Cortex / Q000378:metabolism*; D051379:Mice; D008871:Microvilli / Q000378:metabolism; D008970:Molecular Weight; D009004:Monosaccharide Transport Proteins / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D012964:Sodium / Q000378:metabolism
D009004:Monosaccharide Transport Proteins; D003571:Cytochalasin B; D012964:Sodium; D005947:Glucose