The effect of iron on mammalian cortical neurons in culture.
We added iron in the ferric form to predominantly neuronal, cortical cell cultures, and determined clonazepam-displaceable [3H]diazepam binding, choline acetyltransferase activity, high-affinity [3H]GABA uptake, and glutamic acid decarboxylase activity. Chronic exposure (14 days) to low concentrations (0.01, 0.04, and 0.1 micrograms/ml) of added ferric iron resulted in a significant decrease in each of the measures studied.
D000818:Animals; D002452:Cell Count; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D005290:Ferric Compounds / Q000494:pharmacology*; D005333:Fetus; D005968:Glutamate Decarboxylase / Q000378:metabolism; D007501:Iron / Q000494:pharmacology*; D051379:Mice; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D005290:Ferric Compounds; D005680:gamma-Aminobutyric Acid; D007501:Iron; D002795:Choline O-Acetyltransferase; D005968:Glutamate Decarboxylase