title: Radioiodinated ganglioside GM1: a potential tool for the investigation of ganglioside function in vivo.
abstract: Ganglioside GM1 was labeled with 125I either directly or after conjugation to tyramine. Direct iodination of the sphingosine chain resulted in a compound which dehalogenated rapidly both in vitro and in vivo. The binding of the 125I[GM1-tyramine] conjugate to neuronal membranes in vitro was similar to that reported for 3H-GM1. The biodistribution of 125I activity after intravenous injection of 0.5 uM and 5 uM 125I[GM1-tyramine] in mice was also similar to that reported for 3H-GM1. This radioiodinated GM1 derivative might be useful for the study of ganglioside GM1 function in vivo.
mesh_terms: D000818:Animals; D005677:G(M1) Ganglioside; D007457:Iodine Radioisotopes; D008297:Male; D008823:Micelles; D009474:Neurons / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014018:Tissue Distribution
chemical_list: D007457:Iodine Radioisotopes; D008823:Micelles; D005677:G(M1) Ganglioside