Stopped-flow X-ray scattering device with a slit-type mixer.
A slit-type mixer specially designed for stopped-flow X-ray scattering was constructed. It mixes two solutions at a time, allowing a large-sized window to be used immediately after mixing. Dead time of the mixer was 13 ms with 3.5 kg/cm2 of pressure at the piston head. It has been successfully used for studying the tetramer-dimer interconversion of phosphorylase a. The applicability of this mixer to other detection methods, far-UV circular dichroism, light scattering, EXAFS and fluorescence is also indicated.
D015086:2,6-Dichloroindophenol; D000388:Air; D001205:Ascorbic Acid; D004867:Equipment Design; D010084:Oxidation-Reduction; D011312:Pressure; D012542:Scattering, Radiation / Q000295:instrumentation*; D014965:X-Rays
D015086:2,6-Dichloroindophenol; D001205:Ascorbic Acid