Electrofusion of Chinese hamster ovary cells after ethanol incubation.
Electrofusion of Chinese hamster ovary cells is obtained with cells growing in monolayers on a culture dish. The cells were incubated with increasing amounts of ethanol before the field pulsation. The electroperforation was apparently not affected by the incubation but the threshold field intensity required to induce the fusion was shifted to higher values. This effect is tentatively explained by a decrease in size of the field-induced pores as a consequence of an increase in the lipid matrix fluidity.
D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004560:Electricity; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D008560:Membrane Fluidity / Q000187:drug effects; D008561:Membrane Fusion / Q000187:drug effects*; D010053:Ovary
D000431:Ethanol