Identification of estradiol in the ovaries of the silkworm, Bombyx mori.
Estradiol was extracted and partially purified from the ovaries of the silkworm, Bombyx mori. Identification of estradiol was done by use of radioimmunoassay (RIA) and by gas chromatography-mass spectrometry (GC-MS) after derivatization into the ethyldimethylsilyl derivative. Concentration of estradiol in the ovaries was estimated to be 176 pg/g (RIA) and 63 pg/g (GC-MS).
D000818:Animals; D012831:Bombyx / Q000032:analysis*; D004958:Estradiol / Q000032:analysis*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D010053:Ovary / Q000032:analysis; D011863:Radioimmunoassay
D004958:Estradiol