Functional gap junctions in mouse small intestinal crypts.
We demonstrate intercellular transfer of Lucifer yellow and the existence of gap junctions in isolated mouse small intestinal crypts. The pattern of dye transfer approximates the normal pattern of cell proliferation and differentiation in the intestinal epithelium. These findings indicate that the cells of normal crypts form an effective intercellular continuum. This intercellular route may function in the establishment of chemical, ionic, or electrical fields, which in turn may play a role in the control of cell proliferation, differentiation, and secretion in the crypt.
D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D005614:Freeze Fracturing; D007365:Intercellular Junctions / Q000502:physiology / Q000648:ultrastructure*; D007421:Intestine, Small / Q000166:cytology / Q000648:ultrastructure*; D007546:Isoquinolines; D008297:Male; D051379:Mice
D007546:Isoquinolines; C017475:lucifer yellow