Optimization of laser beams in FRAP experiments of microscopical objects.
In fluorescence recovery after photobleaching (FRAP) experiments the sample is irradiated on a small spot, the diameter of which must be related to the sample size and the diffusion constant to be measured. This paper considers the conventional FRAP set-up where a laser beam is directed through a microscope vertical illuminator to the sample. The requirements of an intermediate optical system producing a Gaussian beam with a waist of given radius in the microscope object plane are considered, and the optical parameters determined.
D002462:Cell Membrane / Q000648:ultrastructure; D007425:Intracellular Membranes / Q000648:ultrastructure; D007834:Lasers; D008051:Lipid Bilayers; D008433:Mathematics; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D009940:Organoids / Q000648:ultrastructure; D010782:Photolysis
D008051:Lipid Bilayers