title: Use of aqueous two-phase partition to detect cell surface changes during growth of Dictyostelium discoideum.
abstract: Changes in the cell surface properties of amoebae of Dictyostelium discoideum during growth in different culture conditions have been studied by aqueous two-phase partitioning on a thin-layer countercurrent distribution apparatus. Changes in cell surface properties were not dependent on the source of nutrients but only on cell density. There was a progressive increase in cell surface hydrophobicity with cell density in both axenic cultures and cultures grown with a bacterial substrate. It is proposed that it is these cell-density-related surface changes that account for the ability of amoebae grown in different conditions to sort out during subsequent development in a manner related to cell fate.
mesh_terms: D001419:Bacteria; D002452:Cell Count; D002462:Cell Membrane / Q000502:physiology*; D002469:Cell Separation; D002478:Cells, Cultured; D003470:Culture Media; D004023:Dictyostelium / Q000254:growth & development*; D005856:Germ-Free Life
chemical_list: D003470:Culture Media