[Synthesis of peptide fragments of influenza virus A/Aichi/2/68 (H3N2) hemagglutinins].
Peptides corresponding to sequences 122-133, 136-147, and 154-164 of the heavy chain of hemagglutinin of the A/Aichi/2/68 (H3N2) influenza virus have been synthesized by stepwise elongation of the peptide chain with Boc-amino acid activated esters or by condensation of peptide blocks by DCC/HOBt-method. A coloured C-protecting group, 2-[4-(phenylazo)-benzylsulfonyl]ethyl (PSE), was used, which is convenient in purification of synthetic peptides. After removal of terminal N-and C-protecting groups the side-protecting residues were cleaved off with 1 M trifluoromethanesulfonic acid in trifluoroacetic acid containing 10% thioanisole. Crude products were purified by preparative reversed-phase liquid chromatography. Synthesized peptides were conjugated with BSA.
D000595:Amino Acid Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D006389:Hemagglutinins, Viral; D053122:Influenza A Virus, H3N2 Subtype; D009980:Influenza A virus / Q000276:immunology*; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000276:immunology
D006389:Hemagglutinins, Viral; D010446:Peptide Fragments
Sintez peptidnykh fragmentov gemaggliutinina virusa grippa A/Aichi/2/68 (H3N2).