Electrical responses of cultured thyroid cells to serotonin.
Cultured porcine thyroid cells, maintained in the differentiated state by dibutyryl cyclic AMP, responded to serotonin (5-HT; 10 nmol/l to 1 mumol/l) with a depolarization of the membrane potential, but did not respond to histamine (100 mumol/l) or dopamine (1 mumol/l). The resting membrane potential of these cells was about -71 mV, maximal concentrations of 5-HT (1 mumol/l) inducing a depolarization to approximately -53 mV. Methysergide or phenoxybenzamine, but not propranolol, abolished the response to 5-HT. Sensitivity to 5-HT was reduced by previous exposure of cultures to TSH, the beta-adrenoceptor agonist salbutamol or 5-HT itself.
D000420:Albuterol / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D004298:Dopamine / Q000494:pharmacology; D006632:Histamine / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D008784:Methysergide / Q000494:pharmacology; D010643:Phenoxybenzamine / Q000494:pharmacology; D012701:Serotonin / Q000494:pharmacology*; D013552:Swine; D013961:Thyroid Gland / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D013972:Thyrotropin / Q000494:pharmacology
D010643:Phenoxybenzamine; D012701:Serotonin; D006632:Histamine; D013972:Thyrotropin; D000420:Albuterol; D004298:Dopamine; D008784:Methysergide