An ELISA to detect antibody specific for surface antigens of parasitic nematodes.
An enzyme immunoassay method is described for the detection of specific immunoglobulins bound to the surface of intact adult Nippostrongylus brasiliensis. Binding of antibody is complete in 1 h and the entire procedure can be completed in less than 4 h. This method has been used to detect the quantity of specific IgG antibodies in sera of rats infected with N. brasiliensis.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D009559:Nippostrongylus / Q000276:immunology*
D000906:Antibodies; D000954:Antigens, Surface