Murine neutrophils collected from subcutaneously implanted sponges.
Polyvinyl sponges implanted subcutaneously in mice were used as a source of murine neutrophils (PMNs). Yield of PMNs from sponges averaged 6.3 X 10(5) PMNs per mouse and viability of sponge PMNs was 96.5%. These results were comparable to the yield and viability of PMNs harvested from peripheral blood. The chemotactic activity of sponge PMNs was significantly greater than the chemotactic activity of peripheral blood PMNs (chemotactic index of 4.78 +/- 1.66 in sponge PMNs versus 2.16 +/- 1.08 in peripheral blood PMNs, P less than 0.001). The difference in chemotactic activity was not attributable to hypotonic injury of PMNs nor the presence of soluble factors in sponge fluids. Phagocytic activity of sponge PMNs was comparable to the activity of blood PMNs (mean of 31.3 +/- 16.4% phagocytic cells for sponge PMNs versus 33.0 +/- 23.9% for blood PMNs). In addition, the number of fluorescent spheres ingested by sponge PMNs was not different (mean of 2.81 +/- 0.86 in sponge PMNs versus 2.65 +/- 0.68 spheres per cell for blood PMNs). These studies indicate that subcutaneously implanted sponges can be used as a source of functioning murine PMNs.
D000818:Animals; D001800:Blood Specimen Collection / Q000379:methods; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002634:Chemotaxis, Leukocyte; D005260:Female; D051379:Mice; D009504:Neutrophils / Q000502:physiology*; D010587:Phagocytosis; D011145:Polyvinyls; D019736:Prostheses and Implants; D013523:Surgical Equipment; D014680:Veins
D011145:Polyvinyls