title: Use of carboxypeptidase Y in studies of the mode of insertion of cytochrome b5 into lipid vesicles.
abstract: Carboxypeptidase Y preparations from baker's yeast have been found to exhibit endopeptidase activity when cytochrome b5 was used as substrate. As the susceptibility of cytochrome b5 to attack by carboxypeptidase Y has been used to distinguish between two modes of insertion of cytochrome b5 into lipid bilayer, one which has the C terminal buried in the lipid bilayer and one which has a free C terminal, caution should be taken when employing carboxypeptidase Y preparations for this type of studies.
mesh_terms: D000818:Animals; D002268:Carboxypeptidases; D055598:Chemical Phenomena; D002621:Chemistry; D003573:Cytochrome b Group; D015786:Cytochromes b5; D004591:Electrophoresis, Polyacrylamide Gel; D008051:Lipid Bilayers; D008099:Liver; D013379:Substrate Specificity; D013552:Swine
chemical_list: D003573:Cytochrome b Group; D008051:Lipid Bilayers; D015786:Cytochromes b5; D002268:Carboxypeptidases; C022633:serine carboxypeptidase