title: Inhibition of specific antibody binding to adult male Schistosoma mansoni by adsorbed host serum components.
abstract: The ability of anti-schistosome antibody to bind to adult male Schistosoma mansoni was studied, using fluoresceinated Staphylococcus aureus to detect specific antigen-antibody interaction at the parasite's surface. Both freshly perfused parasites and parasites which had had their adsorbed host antigens removed by elution were employed in a series of experimental manipulations to ascertain under what conditions specific antibody binding occurs and what conditions or factors are necessary for the parasite to reconstitute its surface so that specific binding is precluded. Neither normal mouse serum nor normal mouse IgG bound in a specific manner to either fresh or eluted worms. Slight binding was noted with immune mouse serum on both fresh and eluted worms, while immune IgG produced weak binding on fresh worms, but strong binding to eluted worms. This strong binding was reduced to the level seen on fresh worms by pre-incubation of the eluted worm in normal IgG prior to incubation in immune IgG and binding was completely negated by pre-incubation in either normal mouse serum or normal mouse serum minus IgG. The binding of immune IgG to eluted worms was not diminished by pre-incubation in mouse albumin, bovine albumin, or fetal bovine serum. These studies demonstrate that adsorbed host serum components can inhibit specific antigen-antibody interaction at the parasite's surface and suggest that a degree of specificity exists in what the parasite adsorbs from the host. These data further suggest that the protective serum factor or factors may include, but are not limited to, host IgG.
mesh_terms: D000327:Adsorption; D000418:Albumins / Q000494:pharmacology; D000818:Animals; D000937:Antigen-Antibody Reactions; D000947:Antigens, Helminth / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D001769:Blood; D006790:Host-Parasite Interactions; D007074:Immunoglobulin G / Q000276:immunology*; D008297:Male; D051379:Mice; D012550:Schistosoma mansoni / Q000276:immunology*; D012710:Serum Albumin, Bovine / Q000494:pharmacology
chemical_list: D000418:Albumins; D000947:Antigens, Helminth; D000954:Antigens, Surface; D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine