DNA cross-links in human leucocytes treated with vinyl acetate and acetaldehyde in vitro.
Human leucocytes were incubated in the presence of vinyl acetate or acetaldehyde (10-20 mM) for 4 h at 37 degrees C in vitro. DNA damage was analysed by alkaline elution. None of the compounds induced a detectable increase in the frequency of DNA strand breaks. Cells exposed to 5 Gy of X-ray immediately after treatment and before alkaline elution showed a clear, dose-dependent retardation of the elution rate in comparison with X-irradiated control cells. These results demonstrate that both vinyl acetate and acetaldehyde induce DNA cross-links in human cells.
D000079:Acetaldehyde / Q000633:toxicity*; D004247:DNA / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007962:Leukocytes / Q000378:metabolism; D014753:Vinyl Compounds / Q000633:toxicity*
D014753:Vinyl Compounds; D004247:DNA; D000079:Acetaldehyde; C011566:vinyl acetate