Formation of hemopoietic-fibroblastoid "flower" colonies by human marrow cells in vitro. Preliminary report.
Marrow cells from 6 patients when cultured in vitro formed macroscopic colonies (0.3-0.8 mm in diameter, 10-28 colonies/10(6) cells) composed of germinal center and peripheral zone, what gave them characteristic "flower" appearance. Germinal center was composed of cells belonging to hemopoietic lineage including lymphocyte-like monocyte-like cells, granulocytes, megakaryoblasts and normoblasts. Peripheral zone was formed by fibroblastoid cells that sometimes contained hemopoietic cells e.g. granulocytes within their cytoplasm. Colonies could be distinguished beginning from day 1 or 2 of culture and by 10 days are composed of at least several thousand cells. The significance of these phenomena is discussed.
D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology*; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans