[Spontaneous formation of mixed-cell colonies in the cultivation of hematopoietic cells on soft agar].
The spontaneous formation of mixed colonies on soft agar was found on the 7-8th days of the bone marrow cell cultivation. The observed colonies differ from those obtained by Fauser and Messner (1980, 1981) in the independence of any stimulators and faster development in the presence of a stromal underlayer. The stromal underlayer has the typical morphological structure: its cells are compactly packed in the form of a skein with stromal mechanocytes among them.
D000362:Agar; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D013997:Time Factors
D000362:Agar
Spontannoe obrazovanie smeshanokletochnykh koloniĭ pri kul'tivirovanii krovetvornykh kletok v miagkom agare.