title: Deoxyribonucleic acids of Corynebacterium genitalium and Corynebacterium pseudogenitalium: their genome molecular weights, base ratios, and DNA relatedness with other corynebacteria involved in urinary tract infections.
abstract: Chromosomal deoxyribonucleic acids of Corynebacterium genitalium and Corynebacterium pseudogenitalium were isolated and analysed spectrophotometrically. Their genome molecular weights ranged from 1.1 X 10(9) to 1.6 X 10(9). The guanine-plus-cytosine content of C. genitalium ranged from 60.0 to 63.3%, whereas that of C. pseudogenitalium ranged from 56.1 to 58.7%. Five strains of C. genitalium showed relatively low levels of DNA relatedness to each other ranging from 35 to 64%. In contrast, most strains of C. pseudogenitalium showed high levels of DNA relatedness to each other ranging from 71 to 89%. Selected strains of C. genitalium and C. pseudogenitalium showed low levels of DNA relatedness (49 to 60%) to other corynebacterial species involved in urinary tract infection. Data obtained in this study indicate that all strains of C. genitalium consist of genetically divergent organisms while the most strains of C. pseudogenitalium belong to a single species.
mesh_terms: D001482:Base Composition; D003352:Corynebacterium / Q000145:classification / Q000235:genetics*; D003354:Corynebacterium Infections / Q000382:microbiology*; D003596:Cytosine / Q000032:analysis; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D006147:Guanine / Q000032:analysis; D006801:Humans; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D013053:Spectrophotometry; D014552:Urinary Tract Infections / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D006147:Guanine; D003596:Cytosine