title: Evidence of synthesis by Lumbricus terrestris of specific substances in response to an immunization with a synthetic hapten.
abstract: Lumbricus terrestris (Annelid, Oligocheta) is capable of cellular- and humoral-specific reactions against natural antigens. Is this earthworm able to elaborate a response of antibody type against a synthetic hapten? L. terrestris have been immunized with conjugates made of one of two different synthetic haptens (400 mw) and a carrier protein: bovine serum albumin (BSA) or keyhole limpet haemocyanin (KLH). The presence of anti-hapten substances in coelomic fluid was tested against each iodinated hapten derivative (125I-hapten). The 125I-hapten-substance complexes were separated from the free derivatives by polyethylene glycol (PEG) 6000 precipitation or by gel filtration. The experiments showed that L. terrestris synthesized specific substances against the immunizing hapten. The importance of the response depended on the carrier protein and on the amount of introduced immunogen. A kinetic study of first and second immunization showed that these substances, elaborated in response to the immunization with the synthetic hapten, were synthesized by cells which kept the immunological memory. These data are discussed in relation to the humoral protection mechanisms already established in the invertebrates.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D004306:Dose-Response Relationship, Immunologic; D006241:Haptens / Q000276:immunology*; D006433:Hemocyanins / Q000276:immunology; D007114:Immunization; D007117:Immunization, Secondary; D007700:Kinetics; D009835:Oligochaeta / Q000276:immunology*; D012710:Serum Albumin, Bovine / Q000276:immunology
chemical_list: D006241:Haptens; D012710:Serum Albumin, Bovine; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin