Fluorometric determination of gizzerosine, a histamine H2-receptor agonist discovered in feedstuffs, employing high-performance liquid chromatography.
Gizzerosine (2-amino-9-(4-imidazolyl)-7-azanonanoic acid) was discovered in fish meal as a causative material of gizzard erosion in chicks. This compound stimulates gastric acid secretion as a histamine H2-receptor agonist and induces gizzard erosion in chicks. A method of fluorometric determination of this compound was developed employing high-performance liquid chromatography. By this method, 0.2 to 5.0 micrograms of gizzerosine in fish meals was quantitated with the recovery of 95-102%. Fish meals, which induced severe gizzard erosion in chicks, contained more than 20 mg/kg of gizzerosine. The present method will be useful in preventing gizzard erosion in chicks, which is a big problem in the poultry industry all over the world.
D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D005396:Fish Products / Q000032:analysis / Q000633:toxicity*; D005506:Food Contamination / Q000032:analysis*; D007093:Imidazoles / Q000032:analysis*; D011201:Poultry Diseases / Q000139:chemically induced / Q000517:prevention & control; D011970:Receptors, Histamine H2 / Q000187:drug effects; D013050:Spectrometry, Fluorescence
D007093:Imidazoles; D011970:Receptors, Histamine H2; C045114:gizzerosine