Purification of fish muscle myosin heavy chain and quantification of the specific polyribosome-bound polypeptide.
A purification procedure for fish myosin heavy chain is described. The protein was injected into rabbits for production of antibodies. The specificity of the antibodies was determined by immunoblotting. The enzyme-linked immunosorbent assay technique was applied to quantify myosin heavy chain bound to isolated polyribosomes of epaxial muscle from fish.
D000818:Animals; D000918:Antibody Specificity; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005399:Fishes; D009132:Muscles / Q000378:metabolism*; D009218:Myosins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D011132:Polyribosomes / Q000378:metabolism
D010455:Peptides; D009218:Myosins