The purification of oligodeoxyribonucleotides with a high guanosine content by anion exchange HPLC.
Anion exchange HPLC purification of synthetic oligonucleotides with a high guanosine content is difficult. This study has demonstrated that these sequences can be successfully purified by anion exchange HPLC using standard conditions provided that the base protecting groups (isobutyryl[ib] and benzoyl[bz]) are retained on the otherwise deprotected oligomer.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D006151:Guanosine; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000302:isolation & purification*
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D006151:Guanosine