Reconstitution of an endocytic fusion event in a cell-free system.
Using a cell-free system we have obtained fusion of vesicles from the endocytic pathway. The fusion is rapid, efficient, and requires ATP. Only vesicles derived from certain positions along the endocytic pathway are capable of fusing. Lysosomes and vesicles derived from the plasma membrane do not fuse.
D000255:Adenosine Triphosphate / Q000502:physiology; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D002474:Cell-Free System; D006224:Cricetinae; D004705:Endocytosis; D009980:Influenza A virus; D007668:Kidney; D008247:Lysosomes / Q000502:physiology; D008561:Membrane Fusion; D008647:Mesocricetus; D012672:Semliki forest virus; D012794:Sialic Acids / Q000032:analysis
D012794:Sialic Acids; D000255:Adenosine Triphosphate