Hybrid cell line from a cloned immunoglobulin-producing mouse myeloma and a nonproducing mouse lymphoma.
A hybrid cell line was established by cell fusion between a cloned Balbic myeloma that is resistant to 8-azaguanine and produces immunoglobulin (gammaG and free kappa chain) and C57BL/6N lymphoma that is resistant to bromo-deoxyuridine and does not produce immunoglobulins. The hybrid cells contained the membrane antigens of both parents; they synthesized free kappa chain; no synthesis of gammaG (gamma(2a)) heavy chain was detected.
D000818:Animals; D002460:Cell Line; D002999:Clone Cells; D006650:Histocompatibility Testing; D006824:Hybridization, Genetic; D007122:Immunoelectrophoresis; D008223:Lymphoma / Q000276:immunology*; D051379:Mice; D009101:Multiple Myeloma / Q000276:immunology*; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000276:immunology / Q000473:pathology; D005719:gamma-Globulins / Q000096:biosynthesis*
D005719:gamma-Globulins