Rapid extraction, radioiodination, and in vivo catabolism of 125I-labeled fibrinogen in the horse.
Two methods were analyzed for the rapid extraction of equine fibrinogen from fresh plasma, using ammonium sulfate-sodium phosphate buffer. Fibrinogen from each of these 2 methods was then radiolabeled with 125I (half-life = 60.2 days, gamma = 35 keV), using monochloroiodine reagent. Mean protein-bound activity was 98.5% and mean clottable radioactivity was 94.1%. Radiolabeled fibrinogen administered IV to 15 horses had an overall mean (+/- SD) plasma half-life of 4.95 +/- 0.44 days.
D000645:Ammonium Sulfate; D000818:Animals; D002021:Buffers; D002712:Chlorides; D005260:Female; D005340:Fibrinogen / Q000302:isolation & purification* / Q000378:metabolism; D006736:Horses / Q000097:blood*; D007454:Iodides; D007457:Iodine Radioisotopes; D008297:Male; D010710:Phosphates
D002021:Buffers; D002712:Chlorides; D007454:Iodides; D007457:Iodine Radioisotopes; D010710:Phosphates; C009813:iodine monochloride; D005340:Fibrinogen; C018279:sodium phosphate; D000645:Ammonium Sulfate