Human monocyte and neutrophil chemotaxis in vitro employing 51Cr-labelled leukocytes.
A modification of an objective technique for measuring leukocyte chemotaxis is described using 51Cr-labelled neutrophils and monocytes. Influence of cell concentration, incubation time, chemotactic factor concentration and gradient, filter pore size and incubation temperature on migrational activity of the studied cells has been investigated. The technique was rigorously standardized in order to optimize the assay conditions. The chemotactic activity is expressed as a ratio of stimulated migration and random migration (chemotactic index = CI). The method is relatively simple, sensitive, and fast. It is suitable as a screening test for abnormal leukocyte chemotaxis.
D000293:Adolescent; D000328:Adult; D000368:Aged; D002452:Cell Count; D002469:Cell Separation / Q000379:methods; D002634:Chemotaxis, Leukocyte; D002860:Chromium Radioisotopes; D005260:Female; D006801:Humans; D008297:Male; D008851:Micropore Filters; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000276:immunology; D013696:Temperature; D013997:Time Factors
D002860:Chromium Radioisotopes; D009240:N-Formylmethionine Leucyl-Phenylalanine