Separation of bacterial luciferase from oxidoreductases by affinity chromatography.
The NADH-dependent and NADPH-dependent oxidoreductase activities associated with bacterial luciferase in Vibrio (Beneckea) harveyi can simultaneously be removed from purified luciferase through a Blue Sepharose CL-6B column. The result achieved with this one-step affinity chromatography is similar to that obtained with two sequential "reverse-affinity" chromatographies.
D002846:Chromatography, Affinity / Q000379:methods*; D038181:FMN Reductase; D008156:Luciferases / Q000302:isolation & purification*; D008163:Luminescent Measurements; D009247:NADH, NADPH Oxidoreductases / Q000302:isolation & purification; D010088:Oxidoreductases / Q000302:isolation & purification*; D012685:Sepharose / Q000031:analogs & derivatives; D014733:Vibrio / Q000201:enzymology*
C017842:cibacron blue-sepharose; D012685:Sepharose; D010088:Oxidoreductases; D008156:Luciferases; D038181:FMN Reductase; D009247:NADH, NADPH Oxidoreductases