Radiation-induced peroxidation of lipid dissolved in organic solvent and its inhibition by alpha-tocopherol and cepharanthine.
Effects of cepharanthine and alpha-tocopherol on radiation-induced peroxidation of lipids dissolved in methanol(MeOH)-chloroform (CHCl3)-H2O(v/v, 2/1/0.8) were examined. alpha-Tocopherol strongly inhibited radiation-induced peroxidation of lipids dissolved in MeOH-CHCl3-H2O. However, cepharanthine exhibited a weak inhibitory action in this system. The change in the absorption spectrum of alpha-tocopherol and cepharanthine by X-irradiation was measured. The reagents were dissolved in 95% EtOH acidified with 20 mM HCl and in MeOH-CHCl3-H2O. alpha-Tocopherol exhibited the change in its absorption spectrum in both systems, and seemed to be oxidized at a high rate by free radicals. However, cepharanthine slightly exhibited the change in its spectrum in MeOH-CHCl3-H2O, but not in acidified EtOH.
D000470:Alkaloids; D044182:Benzylisoquinolines; D004530:Egg Yolk; D008054:Lipid Peroxides; D008081:Liposomes; D008954:Models, Biological; D010713:Phosphatidylcholines / Q000528:radiation effects*; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D014810:Vitamin E; D014965:X-Rays; D052117:Benzodioxoles
D000470:Alkaloids; D044182:Benzylisoquinolines; D008054:Lipid Peroxides; D008081:Liposomes; D010713:Phosphatidylcholines; D012997:Solvents; D014810:Vitamin E; C006947:cepharanthine; D052117:Benzodioxoles