Trypanosoma brucei: five commonly used trypanocides assayed in vitro with a mammalian feeder layer system for cultivation of bloodstream forms.
The in vitro activity of five commonly used trypanocides on bloodstream forms of Trypanosoma brucei brucei TC 221 was examined in 24-well culture plates in the presence of bovine fibroblast feeder layer cells. The minimum effective concentrations determined were as follows: Berenil 1.0 microgram/ml; Samorin 10.0 micrograms/ml; Antrycide dimethylsulfate 0.1 microgram/ml; Arsobal 0.01 microgram/ml; Naganol 1.0 microgram/ml. Contrary to values obtained with other in vitro assays, minimum effective concentrations obtained here were within the range of drug levels reached in blood, plasma, or serum of humans and animals after treatment with curative doses. The trypanocidal activity of Naganol in this assay was of particular interest, since Naganol has been hitherto considered to be inactive in vitro.
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004133:Diminazene / Q000031:analogs & derivatives / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D008549:Melarsoprol / Q000494:pharmacology; D010617:Phenanthridines / Q000494:pharmacology; D011806:Quinolinium Compounds / Q000494:pharmacology; D014344:Trypanocidal Agents; D014346:Trypanosoma brucei brucei / Q000187:drug effects* / Q000254:growth & development
D010617:Phenanthridines; D011806:Quinolinium Compounds; D014344:Trypanocidal Agents; C000702:isometamidium chloride; C005707:quinapyramine; C003915:diminazene aceturate; D004133:Diminazene; D008549:Melarsoprol