title: Evidence against an acetylcholine releasing action of cisapride in the human colon.
abstract: The effect of cisapride (R51619) on intrinsic cholinergic nerve activity of human sigmoid taenia coli muscle strips (taenia) was assessed using radiolabelling techniques. Taeniae previously incubated with [3H]-choline were exposed to cisapride (0.41 and 4.1 microM), placebo solution and electrical field stimulation (EFS; 10 Hz, 1 ms, 200 mA for 480 pulses). Cisapride did not affect unstimulated (basal) release of radioactive material nor the release evoked by EFS. It was concluded that cisapride did not demonstrate a cholinomimetic effect in human sigmoid taenia coli muscle strips.
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D020117:Cisapride; D003106:Colon / Q000187:drug effects* / Q000378:metabolism*; D004558:Electric Stimulation; D006801:Humans; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D010880:Piperidines / Q000494:pharmacology*
chemical_list: D010880:Piperidines; D000109:Acetylcholine; D020117:Cisapride