title: Trace derivatization of cytosine with pentafluorobenzoyl chloride and dimethyl sulfate.
abstract: Methods are being developed for derivatizing trace amounts of DNA adducts for ultimate determination by gas chromatographic techniques. High-pressure liquid chromatography is used to optimize appropriate derivatization reactions for the determination of cytosine. A single vial reaction scheme involves acylation with electrophoric pentafluorobenzoylchloride followed by alkylation with dimethyl sulfate. Currently, the overall yield for this reaction starting with 50 nmole of cytosine is 59 +/- 4.6%.
mesh_terms: D000215:Acylation; D000478:Alkylation; D001565:Benzoates; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003596:Cytosine / Q000031:analogs & derivatives / Q000032:analysis; D004247:DNA / Q000378:metabolism; D009153:Mutagens; D013463:Sulfuric Acid Esters; D013464:Sulfuric Acids; D005464:Fluorobenzenes; D005466:Fluorocarbons
chemical_list: D001565:Benzoates; D003596:Cytosine; D004247:DNA; D009153:Mutagens; D013463:Sulfuric Acid Esters; D013464:Sulfuric Acids; C014737:pentafluorobenzoyl chloride; D005464:Fluorobenzenes; D005466:Fluorocarbons; C047690:pentafluorobenzoylcytosine; C007482:dimethyl sulfate