Quantitative determination of serotonin released from human platelets.
A high-performance liquid chromatographic technique has been used for measuring the amount of endogenous serotonin (5-hydroxytryptamine, 5-HT) released from human platelets stimulated by adenosine diphosphate (ADP) and collagen. Four minutes after challenge both agents induce maximal and similar 5-HT releases corresponding to 14% (ADP) and 17% (collagen) of the intraplatelet 5-HT content. ADP and collagen seem to have different mechanisms of inducing secretion, documented by the different relationship between the release patterns and the optical tracings for aggregation. Endogenous 5-HT release might be useful tool for monitoring states of platelet activation.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003094:Collagen / Q000494:pharmacology; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects; D010976:Platelet Count; D012016:Reference Values; D012701:Serotonin / Q000097:blood*
D007202:Indicators and Reagents; D012701:Serotonin; D000244:Adenosine Diphosphate; D003094:Collagen