Differences of proteins in isolated egg surface after fertilization of Xenopus laevis.
Egg surface proteins of Xenopus laevis were compared between unfertilized and fertilized egg surfaces before the first cleavage. The egg surfaces were isolated in acetone. The macromolecular compositions of egg surfaces were analyzed by two-dimensional gel electrophoresis and were shown to contain at least 30 proteins with molecular weights ranging from 27,000 to 200,000. At 50 min after fertilization, one spot with a molecular weight of 160,000 disappeared and two bands with molecular weights of 190,000 and 180,000 increased gradually after fertilization. Although the disappearance of the spot was not affected by colchicine or cytochalasin B, intensification of the two bands was inhibited completely by the two agents.
D000096:Acetone / Q000494:pharmacology; D000818:Animals; D002462:Cell Membrane; D003078:Colchicine / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D004527:Egg Proteins / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005306:Fertilization; D008565:Membrane Proteins / Q000032:analysis*; D010063:Ovum / Q000032:analysis*; D014982:Xenopus laevis; D015053:Zygote / Q000032:analysis*
D004527:Egg Proteins; D008565:Membrane Proteins; D000096:Acetone; D003571:Cytochalasin B; D003078:Colchicine