Comparative studies of light meromyosin paracrystals derived from red, white, and cardiac muscle myosins.
Tryptic and chymotryptic light meromyosin paracrystals from red and cardiac muscles of rabbit show a negative and positive staining pattern with uranyl acetate and phosphotungstate that sharply differs from that of white muscle light meromyosin paracrystals. The main periodicity of about 430 A is the same regardless of the source of light meromyosin. The results are discussed in terms of the molecular structure and the functional properties of various myosins.
D000085:Acetates; D000818:Animals; D002918:Chymotrypsin / Q000494:pharmacology; D003460:Crystallization; D006651:Histocytochemistry; D008854:Microscopy, Electron; D009124:Muscle Proteins / Q000032:analysis* / Q000302:isolation & purification; D009132:Muscles / Q000032:analysis / Q000166:cytology* / Q000187:drug effects; D009206:Myocardium / Q000032:analysis / Q000166:cytology; D009218:Myosins / Q000032:analysis / Q000302:isolation & purification; D010772:Phosphotungstic Acid; D011817:Rabbits; D013194:Staining and Labeling; D014357:Trypsin / Q000494:pharmacology; D014501:Uranium
D000085:Acetates; D009124:Muscle Proteins; D010772:Phosphotungstic Acid; D014501:Uranium; D002918:Chymotrypsin; D014357:Trypsin; D009218:Myosins