Involvement of intracellular Ca2+ in chemotaxis and metabolic burst by neutrophils: the use of antagonists of intracellular Ca2+.
The effect of TMB-8, quin2, chlortetracycline, chlorpromazine and prenylamine on two neutrophil functions - chemotaxis towards fMLP and metabolic burst activated by PMA or fMLP - was compared. All drugs inhibited both functions in the absence of extracellular Ca2+. The inhibitory effect of all drugs on chemotaxis, and the effect of TMB-8, quin2 and chlortetracycline on the metabolic burst was antagonized by extracellular Ca2+. There were differences between inhibition of chemotaxis and the metabolic burst, and between inhibition of PMA activated and fMLP-activated metabolic burst. The results are consistent with the view that intracellular Ca2+ is required in chemotaxis, and in PMA or fMLP activated metabolic burst, but that the role of Ca2+, or the location where Ca2+ is derived from, is different and depends on function and the type of activator.
D000818:Animals; D002118:Calcium / Q000502:physiology*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D002746:Chlorpromazine / Q000494:pharmacology; D002751:Chlortetracycline / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D066298:In Vitro Techniques; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D009580:Nitroblue Tetrazolium; D010084:Oxidation-Reduction; D011299:Prenylamine / Q000494:pharmacology; D011817:Rabbits
D002121:Calcium Channel Blockers; D009580:Nitroblue Tetrazolium; D003571:Cytochalasin B; D009240:N-Formylmethionine Leucyl-Phenylalanine; D011299:Prenylamine; D002118:Calcium; D002746:Chlorpromazine; D002751:Chlortetracycline