The mechanism of resistance to puromycin and to the puromycin-precursor O-demethyl-puromycin in Streptomyces alboniger.
Ribosomes from Streptomyces alboniger are sensitive in vitro to puromycin and, to a lesser extent, to the puromycin-precursor O-demethyl-puromycin. The puromycin-inactivating enzyme (puromycin N-acetyltransferase) from S. alboniger also N-acetylates O-demethyl-puromycin. This finding indicates that in certain antibiotic-producing organisms the antibiotic-inactivating enzymes may play a role in self-defence against toxic precursor molecules.
D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism; D002855:Chromatography, Thin Layer; D004352:Drug Resistance, Microbial; D010452:Peptide Biosynthesis; D010455:Peptides; D011691:Puromycin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D012270:Ribosomes / Q000187:drug effects; D013302:Streptomyces / Q000187:drug effects* / Q000201:enzymology
D010455:Peptides; C048103:O-demethylpuromycin; C012001:polyphenylalanine; D011691:Puromycin; D000123:Acetyltransferases