Assessment of an in situ rat intestine preparation with perfused vascular bed for studying the absorption and first-pass metabolism of drugs.
The perfused in situ rat jejunum preparation originally described by Hanson and Parsons (1976) was adapted for use in absorption and metabolism studies with drugs. The preparation allows simultaneous perfusion of the gut lumen and associated vasculature and is viable for one hour. The viability of the preparation was assessed, and the application of the method is illustrated by experiments with the opiate analgesic, buprenorphine.
D000818:Animals; D001784:Blood Gas Analysis; D001834:Body Water / Q000378:metabolism; D002047:Buprenorphine / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D066298:In Vitro Techniques; D007408:Intestinal Absorption; D007583:Jejunum / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects; D010477:Perfusion / Q000295:instrumentation; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D007773:Lactates; D004364:Pharmaceutical Preparations; D002047:Buprenorphine; D005947:Glucose