Methods for enzymatic and colorimetric determinations of D-erythrulose (D-tetrulose).
The specific determinations of D-erythrulose by enzymatic assay or colorimetric method, which permit the quantitative determination of between 20 and 400 nmol of the sugar, are described. Enzymatic determination of D-erythrulose made use of the D-erythrulose reductase purified from beef or chicken liver, which catalyzes specifically the reduction of D-erythrulose with concomitant conversion of NADH to NAD+. The colorimetric microdetermination of erythrulose could be carried out by utilizing the phenol-sulfuric acid reaction under low temperature. These methods are simple, rapid, and sensitive, and give reproducible results.
D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002417:Cattle; D002645:Chickens; D003124:Colorimetry / Q000379:methods*; D008099:Liver / Q000201:enzymology; D019800:Phenol; D010636:Phenols; D013401:Sugar Alcohol Dehydrogenases; D013464:Sulfuric Acids; D013780:Tetroses / Q000032:analysis*
D002241:Carbohydrates; D010636:Phenols; D013464:Sulfuric Acids; D013780:Tetroses; D019800:Phenol; C100073:erythrulose; D013401:Sugar Alcohol Dehydrogenases; C020117:D-erythrulose reductase; C033158:sulfuric acid