[Demonstration of the swine fever virus by the infection of cell cultures of lymphocytes and lysed red blood cells].
A method was worked out for the diagnosing of swine fever by means of infecting sensitive cell cultures on lamellae with lyzed red blood cells and lymphocytes, the demonstration being performed through immunofluorescence. The new method made it possible to avoid killing of suspected animals for diagnostic purposes (in order to prepare suspensions of parenchymal organs and isolate the virus) while the characteristic morphologic changes were still not well manifested.
D000818:Animals; D002460:Cell Line; D006691:Classical Swine Fever / Q000175:diagnosis* / Q000382:microbiology; D006692:Classical Swine Fever Virus / Q000302:isolation & purification*; D004912:Erythrocytes / Q000382:microbiology*; D006461:Hemolysis; D008214:Lymphocytes / Q000382:microbiology*; D008856:Microscopy, Fluorescence; D013552:Swine; D013997:Time Factors; D014776:Virus Cultivation / Q000379:methods
Dokazvane na virusa na chumata po svinete chrez zaraziavane na kletŭchni kulturi s limfotsiti i lizirani cherveni krŭvni kletki.