A novel approach to the isolation of rabbit reticulocyte haem-controlled eIF-2 alpha protein kinase.
A new strategy for the purification of rabbit reticulocyte haem-controlled eIF-2 alpha kinase is described, based on the fact that this kinase can be self-phosphorylated in several sites. Incubation of partially purified kinase with ATP changes its behaviour on anion exchangers sufficiently to separate it from almost all contaminating proteins.
D000818:Animals; D002852:Chromatography, Ion Exchange; D006418:Heme / Q000494:pharmacology*; D007700:Kinetics; D010766:Phosphorylation; D011494:Protein Kinases / Q000097:blood* / Q000302:isolation & purification; D011817:Rabbits; D012156:Reticulocytes / Q000201:enzymology*; D019892:eIF-2 Kinase
D006418:Heme; D011494:Protein Kinases; D019892:eIF-2 Kinase