[The process of promutagen biotransformation studied by the Ames test. II. The extent of the manifestation of the mutagenic action of nitrosomorpholine, diethylnitrosamine and cyclophosphane using various subfractions of rat liver homogenate].
The data are presented on involvement of components of microsomal and cytosolic subfractions composing the S-9 fraction of rat liver homogenate in processes leading to formation of active metabolites of nitrosomorpholine (NM), diethyl nitrosoamine (DENA) and cyclophosphane (CP) promutagens and their detoxication resulting from the reaction with glutathione (G-SH) added to the system. It is established that the process of metabolic activation is only connected with microsomal subfraction, while reactions of the first phase of CP and DENA metabolism take place when both microsomal and cytosolic subfractions are added. Decrease in the effect of all promutagens studied under the action of G-SH was observed after microsomal and cytosolic subfractions of the S-9 fraction were introduced into the activating mixture. Various values of dependence of the metabolic activation level and the extent of decrease in the mutagenic action, upon addition of G-SH, on the protein content in microsomal and cytosolic subfractions were obtained.
D000818:Animals; D001711:Biotransformation / Q000187:drug effects; D003520:Cyclophosphamide / Q000378:metabolism / Q000633:toxicity*; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004052:Diethylnitrosamine / Q000378:metabolism / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000378:metabolism / Q000633:toxicity*; D009602:Nitrosamines / Q000378:metabolism / Q000633:toxicity*; D010084:Oxidation-Reduction / Q000187:drug effects; D051381:Rats
D009153:Mutagens; D009602:Nitrosamines; D004052:Diethylnitrosamine; C002741:N-nitrosomorpholine; D003520:Cyclophosphamide; D005978:Glutathione
Issledovanie protsessa biotransformatsii promutagenov s pomoshch'iu testa Eĭmsa. Soobshchenie II. Vyrazhennost' mutagennogo deĭstviia nitrozomorfolina, diétilnitrozamina i tsiklofosfana pri ispol'zovanii razlichnykh subfraktsiĭ gomogenata pecheni krysy.