Studies on antitumor drugs targeting DNA: photosensitive DNA cleavage of copper-camptothecin.
In combination with copper(II) ion and 365 nm-light, anti-tumor alkaloid camptothecin produced remarkable DNA strand scission. The DNA sequencing analysis revealed considerably random nucleotide sequence cleavage. The present DNA breakage reaction was strongly inhibited by catalase and bathocuproine, but not by superoxide dismutase, mannitol, and 1,4-diazabicyclo-[2.2.2]octane. The camptothecin-Cu(II)-UV light system also clearly induced bacteriophage-inactivation which is associated with the DNA degradation. On the basis of the experimental results, the reaction mechanism for the present DNA cleavage has been discussed.
D001483:Base Sequence; D002166:Camptothecin; D002374:Catalase; D003300:Copper; D004247:DNA; D007202:Indicators and Reagents; D010782:Photolysis; D013482:Superoxide Dismutase; D014466:Ultraviolet Rays
D007202:Indicators and Reagents; D003300:Copper; D004247:DNA; D002374:Catalase; D013482:Superoxide Dismutase; D002166:Camptothecin