Bile acids. LXXVII. Large-scale preparation of 5 alpha-anhydrocyprinol from carp bile.
An improved method of preparation of 5 alpha-anhydrocyprinol from large quantities of carp bile is reported. The following materials were obtained by this method: 5 alpha-anhydrocyprinol, 5 alpha-cholestane-3 alpha,7 alpha,12 alpha,25-tetrol, 5 alpha-cholestane 3 alpha,7 alpha,12 alpha,26-tetrol, an unidentified sterol from the neutral lipid fraction, allocholic acid and allochenodeoxycholic acid from the bile acid fraction. Yields of 5 alpha-anhydrocyprinol and bile acids vary in different batches. The identity and purity of these materials were verified by thin-layer chromatography, gas liquid chromatography and gas chromatography-mass spectrometry.
D000818:Animals; D001646:Bile / Q000032:analysis*; D001647:Bile Acids and Salts / Q000032:analysis; D002347:Carps / Q000378:metabolism*; D002777:Cholestanols / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003530:Cyprinidae / Q000378:metabolism*
D001647:Bile Acids and Salts; D002777:Cholestanols; C023378:anhydrocyprinol