Determination of Sepharose-bound protein with Coomassie brilliant blue G-250.
The colorimetric procedure of Bradford (M.M. Bradford, 1976, Anal. Biochem. 72, 248-254) was found to be convenient for determining the content of a protein immobilized on Sepharose. Being simple, sensitive, and rapid, this method appears very useful in studies involving multiple analyses of immobilized protein species present at low concentrations.
D003124:Colorimetry / Q000379:methods; D007202:Indicators and Reagents; D011485:Protein Binding; D011506:Proteins / Q000032:analysis*; D012394:Rosaniline Dyes; D012685:Sepharose
D007202:Indicators and Reagents; D011506:Proteins; D012394:Rosaniline Dyes; D012685:Sepharose; C004692:coomassie Brilliant Blue