title: Investigations on the deformability of human red blood cells stored in different preservative solutions.
abstract: Deformability changes of red blood cells stored either as whole blood or as different resuspensions in sucrose-free or sucrose-containing media were characterized by measuring red cell filterability during 36 days of storage. For whole blood and SAG-resuspensions only a slight increase of the filterability index was observed during the first 3 weeks; after 36 days it was elevated 1.4-fold (whole blood) and 1.8-fold (SAG-resuspension). In contrast, a strong deformability loss (increase of the filterability index to more than the 6-fold) was observed for red blood cells stored in sucrose-containing CDS-AG medium. This deformability loss is caused by an increase of the internal viscosity (at least 4-fold) due to the shrinkage of red blood cells by more than 20%. After incubation for 1 h in fresh plasma no changes of filterability could be found. The in vivo behavior of such rigid cells is discussed with regard to references about the posttransfusion survival.
mesh_terms: D001793:Blood Preservation / Q000379:methods*; D004907:Erythrocyte Deformability; D006801:Humans; D066298:In Vitro Techniques; D012996:Solutions; D013395:Sucrose; D013997:Time Factors
chemical_list: D012996:Solutions; D013395:Sucrose