[Fibronectin-synthesizing activity of free and membrane-bound polyribosomes from human fibroblasts and chick embryos].
Using immunochemical methods, the fibronectin-synthesizing activity of membrane-bound and free polyribosomes in a cell-free system was studied. It was demonstrated that fibronectin biosynthesis on membrane-bound polyribosomes from human embryonic fibroblasts makes up to 4.9%, while that from chicken embryos--1.1% of the total amount of the de novo synthesized proteins (as compared to 1.0 and 0.3% in free polyribosomes, respectively). Fibronectin monomers (Mr = 330 kD) were detected only in the newly synthesized (in the presence of spermidine) material of the cell-free system containing heavy fractions of membrane-bound polyribosomes.
D000818:Animals; D002474:Cell-Free System; D002478:Cells, Cultured; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000378:metabolism; D005353:Fibronectins / Q000096:biosynthesis*; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D011132:Polyribosomes / Q000378:metabolism*; D011233:Precipitin Tests; D013095:Spermidine / Q000494:pharmacology
D005353:Fibronectins; D013095:Spermidine
Fibronektinsinteziruiushchaia aktivnost' svobodnykh i membransviazannykh poliribosom iz chelovecheskikh fibroblastov i kurinykh émbrionov.