Comparison between IgM-IFA and DS-IgM-ELISA performed on IgM fractions and whole sera in acute acquired toxoplasmosis.
The present investigation has shown that antibodies titers obtained by IgM-IFA performed on pure IgM-fractions, obtained by gel-filtration method, correlate well with DS-IgM-ELISA. False negative IgM-IFA results that frequently occurs with sera having greater than or equal to 200 I.U./ml by IgG-IFA, were not observed with DS-IgM-ELISA: and the 98% of sera positive only after separation of IgM fraction with IgM-IFA, are also positive with DS-IgM-ELISA performed on whole serum.
D002850:Chromatography, Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005188:False Negative Reactions; D005189:False Positive Reactions; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D008722:Methods; D014123:Toxoplasmosis / Q000175:diagnosis* / Q000276:immunology
D007075:Immunoglobulin M