title: An evaluation of tubulin as a molecular clock.
abstract: The available sequence data for tubulin indicates that it cannot be used as a molecular clock. Apparent alpha-tubulin mutation rates, for example, vary from 0.16 to 3.8 PAMs per 100 million years depending on which two alpha-tubulins are compared. All animal alpha-tubulin mutation rates seem to be quite low, whereas those of non-animals are relatively high. A similar division is not present amongst the beta-tubulins; their apparent mutation rates, however, vary just as much. For any given tubulin, the largest number of amino acid sequence differences are obtained when comparing it to the tubulins of yeasts. Sequence comparisons with the tubulins of unicellular algae and chelates show far fewer differences. Cytochrome c data, however, show that the ciliates diverged from animals well before the yeasts. This means, therefore, that the average tubulin mutation rates in yeasts and ciliates since the time they shared a common ancestor must be quite different. The high mutation rate of yeast tubulins may possibly reflect the absence of cilia. Structural constraints imposed on tubulin by the large number of interactions with other components of the complex ciliary axoneme probably have a significant effect on its rate of mutation.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005075:Biological Evolution; D006801:Humans; D008870:Microtubules / Q000378:metabolism; D009154:Mutation; D013997:Time Factors; D014404:Tubulin / Q000235:genetics*; D015003:Yeasts / Q000235:genetics
chemical_list: D014404:Tubulin