Contraluminal phosphate transport in the proximal tubule of the rat kidney.
In order to study the characteristics of contraluminal phosphate transport the stopped flow microperfusion technique [13] has been applied. By measuring the time-dependent decrease of interstitial 33Pi concentration at different starting concentrations a simple diffusion kinetics with a permeability coefficient of 7.5 +/- 1.0 X 10(-8) cm2 s-1 was found. Such a kinetic was so far only observed with 2-deoxy-D-glucose. This substance, however, is transported in addition by facilitated diffusion as was seen by paraaminohippurate, methylsuccinate and sulfate. The contraluminal transport of phosphate was inhibited by H2-DIDS (5 mmol/l). It was, however, not influenced by omission of Na+ from the perfusates, by addition of sulfate (150 mmol/l), methylsuccinate (50 mmol/l), arsenate (50 mmol/l), the Hg-compound mersalyl (5 mmol/l), high and low phosphate diet and pH changes between 6.0 and 8.0. The data indicate that phosphate, which is reabsorbed from the lumen by a Na+-dependent transport system, leaves the cell by a rather unspecific contraluminal diffusion pathway.
D000818:Animals; D001693:Biological Transport, Active; D003847:Deoxyglucose / Q000378:metabolism; D004032:Diet; D004058:Diffusion; D007687:Kidney Tubules, Proximal / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D010710:Phosphates / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D010710:Phosphates; D003847:Deoxyglucose