Freeze-dried vaccine against Rinderpest: stability and activity study.
A freeze-dried vaccine against Rinderpest was prepared from modified virus multiplied in calf kidney cell culture. Characteristics of the vaccine are as follows: high titre after freeze-drying (10(4) CCID50/dose), well-adapted freeze-drying stabilizer which ensures maintenance of the infective titre of the vaccinal virus, even under severe conditions (3.5 days at +45 degrees C), use of an appropriate solvent: magnesium sulphate molar solution or more simply physiological saline (for stability after reconstitution even at high temperatures--up to 4 h at +45 degrees C). The activity of the vaccine, tested in cattle by antibody titration and resistance to specific challenge perfectly satisfies requirements set by the WHO and OIE.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002417:Cattle; D005602:France; D005612:Freeze Drying; D008278:Magnesium Sulfate; D012301:Rinderpest / Q000382:microbiology / Q000517:prevention & control*; D012302:Rinderpest virus / Q000276:immunology*; D012529:Saudi Arabia; D012965:Sodium Chloride; D012997:Solvents; D014613:Vaccines, Attenuated / Q000276:immunology / Q000592:standards; D014765:Viral Vaccines / Q000276:immunology / Q000592:standards*
D000914:Antibodies, Viral; D012997:Solvents; D014613:Vaccines, Attenuated; D014765:Viral Vaccines; D012965:Sodium Chloride; D008278:Magnesium Sulfate