Implication of the "4S" polycyclic aromatic hydrocarbon binding protein in the transregulation of rat cytochrome P-450c expression.
A protein which specifically binds [3H]benzo[a]pyrene and other polycyclic aromatic hydrocarbons has been purified over 6000-fold from rat hepatic cytosol by using ion-exchange, gel permeation, and hydrophobic interaction chromatography. The binding protein differs from the 9S binding protein characterized in other laboratories. A Stokes radius of 2.75 nm was determined by gel filtration on Sephadex G-100. A sedimentation coefficient of 3.3 S was determined by using sucrose gradient analysis. The ability of this protein to bind total rat liver DNA as well as subclones containing portions of the rat cytochrome P-450c gene was investigated. Under high stringency conditions, this binding protein was found to interact in a specific and saturable manner with several subclones of the rat cytochrome P-450c gene containing 5'-upstream sequences, as well as portions of intron 1. Binding was not observed to the coding portions of the gene. These data implicate the "4S" binding protein in the transregulation of rat cytochrome P-450c expression.
D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D008297:Male; D008970:Molecular Weight; D011083:Polycyclic Compounds / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D002352:Carrier Proteins; D011083:Polycyclic Compounds; D004247:DNA; D003577:Cytochrome P-450 Enzyme System