Simultaneous determination of serum cations, anions and uremic toxins by ion chromatography using an immobilized enzyme.
A procedure is described for the analysis of serum cations and uremic toxins by simultaneous conductivity (CD) and ultraviolet (UV) photometric detection and using phosphoric acid as the eluent. In addition, studies were made on the determination of urea in serum using immobilized urease, and on the simultaneous determination of cationic and anionic compounds. The ammonium ion produced from urea by immobilized urease was determined by CD. No significant differences in amounts were found between the present method and conventional colorimetric methods. There was no significant reduction in immobilized urease activity after two months of continuous use. Simultaneous analysis of cationic and anionic compounds in serum was performed by switching valves in order to change the eluents and to change the channel to the analytical column. No marked base-line variation was observed and satisfactory recovery of anions was achieved.
D000641:Ammonia / Q000097:blood; D000838:Anions / Q000097:blood*; D002412:Cations / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003124:Colorimetry; D003404:Creatinine / Q000097:blood; D004800:Enzymes, Immobilized; D006801:Humans; D007202:Indicators and Reagents; D008760:Methylguanidine / Q000097:blood; D011188:Potassium / Q000097:blood; D014118:Toxins, Biological / Q000097:blood*; D014511:Uremia / Q000097:blood*
D000838:Anions; D002412:Cations; D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D014118:Toxins, Biological; C026865:uremia middle molecule toxins; D008760:Methylguanidine; D000641:Ammonia; D003404:Creatinine; D011188:Potassium