Investigation of the hyaluronic acid-copper complex by N.M.R. spectroscopy.
Analysis of the 13C and 1H relaxation data of the hyaluronic acid-copper complex indicates a binding site involving the carboxyl group and O-1 of the D-glucuronic acid moiety. The paramagnetic relaxation of Cu2+ is discussed within the framework of the Solomon-Bloembergen formalism and it is shown that various atoms experience, in addition to the dipolar paramagnetic relaxation, a strong scalar relaxation contribution. E.s.r. spectra have also been obtained in order to determine the binding constants, and measurements at 69 K gave the g-values of the complex.
D001665:Binding Sites; D002236:Carbohydrate Conformation; D003300:Copper; D006820:Hyaluronic Acid; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008958:Models, Molecular
D003300:Copper; D006820:Hyaluronic Acid