[Inhibition of enzymes of insecticide detoxication in insects by an alkylating analog of a cytochrome P-450 substrate].
It was shown that the alkylating analog of cytochrome P-450 substrate - 4-bromomethyl-2,2,5,5-tetramethyl-3-imidazoline-3-oxide-1-oxyl effectively inhibits in vitro the activities of monooxygenases, glutathione-S-transferases and esterases from the abdomen of the house fly Musca domestica L. The non-alkylating analog (RH) appeared to be a very weak inhibitor of these enzymes. It was demonstrated that the inhibitory action of the alkylating analog consists in its covalent binding to the enzyme protein. Topical application of the cytochrome P-450 alkylating analog on insects led to a decrease of the enzyme activity by approximately 20%.
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000037:antagonists & inhibitors*; D001579:Benzopyrene Hydroxylase / Q000037:antagonists & inhibitors*; D003497:Cyclic N-Oxides; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004950:Esterases / Q000037:antagonists & inhibitors*; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors*; D006793:Houseflies / Q000201:enzymology*; D007093:Imidazoles / Q000378:metabolism / Q000494:pharmacology*; D066298:In Vitro Techniques; D008658:Inactivation, Metabolic; D007306:Insecticides / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
D003497:Cyclic N-Oxides; D007093:Imidazoles; D007306:Insecticides; C047495:4-bromomethyl-2,2,5,5-tetramethyl-3-imidazoline-3-oxide-1-oxyl; C047496:4-iodomethyl-2,2,5,5-tetramethyl-3-imidazoline-3-oxide-1-oxyl; D003577:Cytochrome P-450 Enzyme System; D001579:Benzopyrene Hydroxylase; D001189:Aryl Hydrocarbon Hydroxylases; D005982:Glutathione Transferase; D004950:Esterases
Ingibirovanie fermentov detoksikatsii insektitsidov u nasekomykh alkiliruiushchim analogom substrata tsitokhroma P-450.