[Deformability of thawed erythrocytes: introduction of a new preservative solution].
Recently, KANE et al. (Centre de Transfusion Sanguine, Strasbourg) designed an original preservative medium, called ESOC, allowing a prolonged storage of thawed RBC. We studied on 15 days the evolution of thawed RBC deformability, while RBC where kept preserved, on the one hand in this ESOC solution, on the other hand in physiologic water, without any preservative medium. We tried to correlate this rheological evolution with cellular ATP, cellular 2,3-DPG and membrane proteins evolution. Deformability was measured by filterability with an Hemorheometre. The results are given as a rigidity index, IR. In ESOC, IR and cellular ATP stay in normal values during the 15 days. In physiologic water, deformability decreases strongly and IR is out of normal values after the fifth day. Cellular ATP decreases out of normal values as soon as the third day. 2,3-DPG decreases in both media. Membrane proteins electrophoresis does not show any difference neither in ESOC nor in physiological water, all fifteen days long. We only observed parallelism between deformability and cellular ATP and IR was higher than normal values. We found no correlation between deformability and 2,3-DPG. We also can conclude, with this study, that ESOC allows a good preservation of thawed RBC and by this way complies with the needs for delayed transfusion in current practice.
D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate / Q000097:blood; D001793:Blood Preservation / Q000379:methods; D003451:Cryoprotective Agents; D004163:Diphosphoglyceric Acids / Q000097:blood; D004912:Erythrocytes / Q000166:cytology*; D005615:Freezing; D006801:Humans; D008565:Membrane Proteins / Q000097:blood; D012996:Solutions
D003451:Cryoprotective Agents; D004163:Diphosphoglyceric Acids; D008565:Membrane Proteins; D012996:Solutions; D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate
Déformabilité des érythrocytes décongelés. Apport d'une nouvelle solution protectrice.