Aromatic and nitrogen-containing acid separation by reversed-phase high performance liquid chromatography and identification by photodiode array detector.
A new method of separation and identification of aromatic and nitrogen-containing acids is presented. Fifty-seven different acid standards were separated by reversed-phase high performance liquid chromatography (HPLC). Detection and identification of the acids by ultraviolet (UV) spectra using a photodiode array detector (DAD) is demonstrated. Peak height ratios are reported and complete UV spectra of these acids are displayed. Possible applications of this technique are demonstrated using patients' urines.
D000085:Acetates / Q000302:isolation & purification*; D001565:Benzoates / Q000302:isolation & purification*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D009584:Nitrogen / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet
D000085:Acetates; D001565:Benzoates; D007202:Indicators and Reagents; D009584:Nitrogen