A new method for the growth of myeloid progenitor cells on nitrocellulose paper.
A new method has been developed by which human myeloid progenitor cells can be grown on nitrocellulose paper. The method uses human placental conditioned medium for colony-stimulating activity in an agar layer while the cells grow on the overlying nitrocellulose paper in compact colonies containing granulocytic and macrophage cells. The importance of this method is discussed in the light of its usefulness in carrying out molecular studies on differentiating hematopoietic cells.
D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002478:Cells, Cultured; D003101:Collodion; D003470:Culture Media; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D010920:Placenta / Q000502:physiology
D003470:Culture Media; D003101:Collodion