Phospholipid dependence of Wrb antigen expression in human erythrocyte membranes.
Phospholipid depletion of human erythrocyte membranes by phospholipase A2 modification markedly decreased binding of two monoclonal antibodies to the Wrb (Wright) blood group determinant on glycophorin A. Binding of seven monoclonal antibodies to other glycophorin A determinants, including anti-M and anti-N, was unaffected by phospholipid depletion. These results indicate that Wrb antigen expression is phospholipid-dependent in human erythrocyte membranes.
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D001789:Blood Group Antigens / Q000032:analysis*; D004910:Erythrocyte Membrane / Q000276:immunology*; D006384:Hemagglutination; D006801:Humans; D007074:Immunoglobulin G; D010741:Phospholipases A; D054467:Phospholipases A2; D010743:Phospholipids / Q000097:blood*; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D001789:Blood Group Antigens; D007074:Immunoglobulin G; D010743:Phospholipids; D010741:Phospholipases A; D054467:Phospholipases A2