Dissociation and reassembly of Escherichia coli outer membrane and of lipopolysaccharide, and their reassembly onto flagellar basal bodies.
Purified lipopolysaccharide vesicles dissociate when treated with ethylenediaminetetraacetic acid (EDTA) and then reassemble when dialyzed against Mg(2+). Purified outer, lipopolysaccharide membrane (L membrane) is partially dissociated by treatment with EDTA and fully dissociated upon further treatment with Triton X-100. Both the partially and fully dissociated L membrane can be reassembled by dialysis against Mg(2+). Reassembly of lipopolysaccharide or L membrane in the presence of intact flagella results in specific attachment of flagellar basal bodies to vesicles via the L and sometimes the M ring. Lipopolysaccharide and L membrane appear to be composed of substructures bound together by both Mg(2+) (divalent cation)-mediated and hydrophobic bonds.
D001426:Bacterial Proteins / Q000032:analysis; D001669:Biochemical Phenomena; D001671:Biochemistry; D002462:Cell Membrane / Q000032:analysis; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D003956:Dialysis; D004492:Edetic Acid; D004926:Escherichia coli / Q000032:analysis / Q000166:cytology; D005407:Flagella; D008070:Lipopolysaccharides / Q000032:analysis / Q000302:isolation & purification; D008274:Magnesium; D008854:Microscopy, Electron; D010636:Phenols; D010772:Phosphotungstic Acid; D011523:Protoplasts; D012997:Solvents; D013194:Staining and Labeling; D013501:Surface-Active Agents
D001426:Bacterial Proteins; D002712:Chlorides; D008070:Lipopolysaccharides; D010636:Phenols; D012997:Solvents; D013501:Surface-Active Agents; D010772:Phosphotungstic Acid; D002586:Cesium; D004492:Edetic Acid; D008274:Magnesium