Separation by high-performance liquid chromatography of (3R)- and (3S)-beta-leucine as diastereomeric derivatives.
For studies on the coenzyme B12-dependent enzyme, leucine-2,3-aminomutase, (3R)- and (3S)-beta-leucines were synthesized. The 10-camphorsulfonamide p-nitrobenzyl esters could be resolved by normal-phase HPLC. A much better separation was obtained by reversed-phase HPLC of the diastereomeric derivatives obtained by treatment of -leucine with Marfey's reagent (N2-(5-fluoro-2,4-dinitrophenyl)-L-alaninamide).
D000409:Alanine / Q000031:analogs & derivatives*; D000591:Amino Acid Isomerases / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004136:Dinitrobenzenes; D019751:Intramolecular Transferases; D007930:Leucine / Q000031:analogs & derivatives* / Q000302:isolation & purification; D013237:Stereoisomerism
D004136:Dinitrobenzenes; C027611:beta-leucine; C047134:Marfey's reagent; D000591:Amino Acid Isomerases; D019751:Intramolecular Transferases; C021507:leucine 2,3-aminomutase; D007930:Leucine; D000409:Alanine