title: Wound fluid angiogenesis factor stimulates the directed migration of capillary endothelial cells.
abstract: During wound healing, new capillaries grow into the wound site. An angiogenesis factor isolated from wound fluid stimulates the movement of capillary endothelial cells in a filter migration assay. Experiments were carried out to determine whether the movement seen in the assay was chemokinetic or chemotactic. Capillary endothelial cells were plated onto a collagen-coated coverslip and inverted over a visualization apparatus. Cells exposed to a constant concentration of wound fluid angiogenesis factor (WAF) were more mobile than cells not exposed to WAF, and this movement was chemokinetic. When exposed to a gradient of WAF, the cells translocated toward the higher concentration; this directional movement was chemotactic. Cells in a gradient of WAF morphologically aligned with the gradient. These data support the idea that wound healing angiogenesis is regulated by the chemotaxis of capillary endothelial cells.
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000502:physiology*; D000818:Animals; D002196:Capillaries / Q000166:cytology; D002465:Cell Movement; D002633:Chemotaxis; D004305:Dose-Response Relationship, Drug; D004727:Endothelium / Q000502:physiology*; D006133:Growth Substances / Q000502:physiology*; D066298:In Vitro Techniques; D011817:Rabbits; D014945:Wound Healing
chemical_list: D043925:Angiogenesis Inducing Agents; D006133:Growth Substances