Brugia malayi: clearance of microfilaremia induced by diethylcarbamazine independently of antibody.
The microfilaricidal effect of diethylcarbamazine was studied in the murine model of Brugia malayi microfilaremia. CFI mice with circulating microfilariae were treated with either diethylcarbamazine (6 mg kg-1 day-1) for 10 days or with normal saline. Although antibodies against crude microfilarial antigen were detected in both groups at the time of completion of therapy, the chemically induced microfilarial clearance occurred prior to the development of these antibodies. In addition, the passive transfer of antibody just prior to chemical treatment did not enhance diethylcarbamizine-induced microfilarial clearance. In vitro studies confirmed previous observations that diethylcarbamazine enhanced antibody-dependent neutrophil adherence. Microfilarial clearance was found to be due to trapping and lysis in the liver on pathologic examination. These studies demonstrate that diethylcarbamazine leads to microfilarial clearance in the liver by mechanisms that are not dependent on host antibody.
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002009:Brugia / Q000254:growth & development; D004049:Diethylcarbamazine / Q000627:therapeutic use*; D004605:Elephantiasis, Filarial / Q000188:drug therapy* / Q000276:immunology; D008099:Liver / Q000469:parasitology; D008209:Lymphedema / Q000188:drug therapy*; D051379:Mice; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology / Q000469:parasitology
D000906:Antibodies; D004049:Diethylcarbamazine