Retinyl ester synthesis by the isolated perfused-ventilated neonatal rabbit lung.
Retinyl ester is present in lung but it is unknown if retinyl ester synthesis occurs in that organ. In this study, [3H]-Retinol was perfused into the pulmonary artery of isolated-perfused-ventilated neonatal rabbit lungs. Alumina chromatography was used to separate retinol from retinyl ester in hexane extracts of lung tissue. [3H]-Retinyl ester synthesis did occur and was perfusion time and perfusate [3H]-retinol concentration dependent. Documentation of [3H] retinyl ester synthesis was also made by HPLC analysis of the retinyl ester fraction before and after methanolic KOH hydrolysis. Isolated lung clearly can synthesize retinyl ester.
D000818:Animals; D000831:Animals, Newborn; D004224:Diterpenes; D007700:Kinetics; D008168:Lung / Q000378:metabolism*; D010477:Perfusion; D011817:Rabbits; D000084562:Retinyl Esters; D014316:Tritium; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism*
D004224:Diterpenes; D000084562:Retinyl Esters; D014316:Tritium; D014801:Vitamin A; C014794:retinol palmitate