title: [Comparative determination of norethisterone in the blood plasma by radioimmunological and chromato-mass fragmentographic methods].
abstract: Radioimmunoassay (RIA) and two variants of the chromato-mass fragmentographic (CMF) method were used for comparative determination of norethisterone (NET) in the blood plasma. A deuterated internal standard, capillary gas-liquid chromatography and chromatographed derivates: 3-enol-3-tret-butyldimethylsilyl-17-tri-methysilyl and 3-enol-3,17-bis-tret-butyldimethylsilyl NET esters were used. Both derivates provide for CMF determination after simplified variants. They were used as references for RIA determination of NET. Comparison of the CMF and RIA results indicate to the significance of RIA determination (r = 0.979) while employing antiserum to NET conjugate 3-(0-carboxymethyl)-oxime with bovine serum albumin injected to rabbits.
mesh_terms: D005069:Evaluation Studies as Topic; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008297:Male; D009640:Norethindrone / Q000097:blood*; D011863:Radioimmunoassay / Q000379:methods*; D012015:Reference Standards
chemical_list: D009640:Norethindrone
vernacular_title: Sravnitel'noe opredelenie norétisterona v plazme krovi radioimmunologicheskim i khromato-mass-fragmentograficheskim metodami.