Solubilisation and fractional precipitation of a steroid alpha-ketol oxidase.
An alpha-ketol oxidase that converts deoxycorticosterone to pregnenoic acid has been solubilised and fractionally precipitated with polyethylene glycol from rabbit liver microsomes. Maximal activity was obtained with the 12-14% fraction. Activity was linear with cytochrome P-450 concentrations up to 0.2 nmol and was inhibited with carbon monoxide.
D000445:Aldehyde Oxidoreductases / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D000736:Androstenes / Q000096:biosynthesis; D000818:Animals; D002248:Carbon Monoxide / Q000494:pharmacology; D011232:Chemical Precipitation; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003573:Cytochrome b Group / Q000378:metabolism; D015786:Cytochromes b5; D005260:Female; D066298:In Vitro Techniques; D007535:Isomerases / Q000302:isolation & purification*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D011374:Progesterone / Q000031:analogs & derivatives / Q000096:biosynthesis; D011506:Proteins / Q000378:metabolism; D011817:Rabbits; D012995:Solubility; D013251:Steroid Isomerases / Q000037:antagonists & inhibitors / Q000302:isolation & purification*
D000736:Androstenes; D003573:Cytochrome b Group; D011506:Proteins; C010952:4-pregnene-3,20-dione-21-oic acid; C007686:5-etienic acid; D011374:Progesterone; D002248:Carbon Monoxide; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; D000445:Aldehyde Oxidoreductases; C047769:steroid alpha-ketol oxidase; D007535:Isomerases; C047770:corticosteroid side-chain-isomerase; D013251:Steroid Isomerases