Effect of phospholipids and detergents on transitions and equilibrium between the bleaching intermediates of rhodopsin.
The thermal stability of bleaching intermediates and the metarhodopsins I in equilibrium II equilibrium have been examined for purified rhodopsin in cholic acid and a series of alkyl sucrose esters. The results are compared with rhodopsin contained in its natural phospholipid environment. It is found that detergents modify the transition temperatures of the photointermediates in a complex manner. The equilibrium between metarhodopsins I and II is also strongly dependent on the detergent species. It is shifted according to the micellar rigidity. This effect is reversible and allows direct observation of a back reaction from metarhodopsin II to I.
D000818:Animals; D002417:Cattle; D003902:Detergents / Q000494:pharmacology*; D010743:Phospholipids / Q000494:pharmacology*; D010786:Photoreceptor Cells / Q000378:metabolism; D012168:Retinal Pigments / Q000378:metabolism*; D012243:Rhodopsin / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013501:Surface-Active Agents / Q000494:pharmacology*; D013696:Temperature
D003902:Detergents; D010743:Phospholipids; D012168:Retinal Pigments; D013501:Surface-Active Agents; D012243:Rhodopsin