[Effects of Young's solution (YNG solution) on cardiac function and mitochondrial function during one hour ischemia at 30 degrees C and after reperfusion].
In a previous paper from this laboratory, protective effects of YNG solution on the myocardial mechanical function and metabolism of the heart arrested at 10 degrees C ascribable to the presence of Mg2+ were demonstrated. In order to further clarify the role played by Mg2+ in the protection, the present experiment was conducted using the isolated perfused guinea pig heart arrested at 30 degrees C. While the time to arrest of the contraction was equally short both with YNG (K+ + Mg2+) and K (K+) solutions, the time to resumption of contraction was significantly longer with K solution than with YNG solution, and the regular contraction was not resumed with the former solution. The recovery of the coronary flow, left ventricular pressure and dp/dt after reperfusion was around 100% with YNG solution, while the recovery was significantly poorer with K solution. Although the mitochondrial function was well maintained during the arrest both with YNG and K solution, the recovery of the mitochondrial function after reperfusion was observed only with YNG solution; severe damage was noted in mitochondria of the hearts arrested with K solution. There was a good correlation between the changes in mitochondrial function and those in cardiac function.(ABSTRACT TRUNCATED AT 250 WORDS)
D000818:Animals; D002951:Citrates / Q000494:pharmacology*; D004734:Energy Metabolism / Q000187:drug effects; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects*; D006324:Heart Arrest, Induced; D008278:Magnesium Sulfate / Q000494:pharmacology*; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects; D009200:Myocardial Contraction / Q000187:drug effects; D009388:Neostigmine / Q000494:pharmacology*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D011188:Potassium / Q000494:pharmacology*; D019357:Potassium Citrate; D013997:Time Factors
D002951:Citrates; C042312:Young's cardioplegic solution; D009388:Neostigmine; D008278:Magnesium Sulfate; D019357:Potassium Citrate; D011188:Potassium