Elimination of trinitrophenol-specific antibody response by antigen-toxin conjugates.
The aim of the experiments presented here is the selective inhibition of an antigen-specific immune response. Antigen receptors are used as targets for conjugates of antigens and toxin to eliminate antigen-reactive cells. The trinitrophenol (TNP)-specific immune response can be specifically abrogated by incubating the TNP-keyhole limpet haemocyanin (KLH)-primed spleen cells with TNP-ricin or TNP-chicken IgG-ricin conjugates before in vitro stimulation with TNP-KLH. The rate of elimination is dose-dependent and related to the degree of TNP moieties bound to the toxin molecule. The specificity of the toxin conjugates is demonstrated by treating sheep erythrocyte-primed spleen cells with TNP-IgG-ricin conjugates. These results may have therapeutic relevance for treating autoimmune diseases.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D000918:Antibody Specificity; D000921:Antibody-Producing Cells / Q000276:immunology*; D006241:Haptens; D006433:Hemocyanins / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D009578:Nitrobenzenes / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell / Q000276:immunology; D012276:Ricin / Q000008:administration & dosage* / Q000031:analogs & derivatives; D014301:Trinitrobenzenes / Q000008:administration & dosage / Q000276:immunology*
D006241:Haptens; D007074:Immunoglobulin G; D009578:Nitrobenzenes; D011947:Receptors, Antigen, B-Cell; D014301:Trinitrobenzenes; D012276:Ricin; D006433:Hemocyanins