Increased concentrations of basement membrane collagen fragment in urine of diabetic mice.
Levels of a urinary component of basement membrane collagen (type IV) were compared in diabetic (db/db) and normal (db/+) C57BL/Ks inbred mice. A modification of the enzyme-linked immunosorbent assay (ELISA) technique was used with antigen and antibody preparations previously defined to be specific for a fragment from human basement membrane collagen. Significantly elevated levels of basement membrane collagen were found in the urine of homozygous diabetic (db/db) mice as soon as the fourth week after birth. These levels were consistently higher than those found in age-matched normal (db/+) mice throughout the 28 weeks in which measurements were made. Results described here support previous evidence that increased basement membrane collagen synthesis occurs in the db/db model of diabetic nephropathy.
D000818:Animals; D001485:Basement Membrane / Q000378:metabolism; D003094:Collagen / Q000652:urine*; D003921:Diabetes Mellitus, Experimental / Q000235:genetics / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay; D005808:Genes, Recessive; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D051379:Mice; D008817:Mice, Mutant Strains; D010446:Peptide Fragments / Q000652:urine*
D010446:Peptide Fragments; D003094:Collagen