A simple re-embedding method for the preparation of testicular cell cultures for light and electron microscopy.
A simple and reproducible method is described for the preparation of cell cultures for light and transmission electron microscopy. Mixed testicular cells are grown on collagen layered, PTFE-coated coverslips. Thin sheets of plastic-embedded cells are packed together and re-embedded permitting the examination of sections cut perpendicular to the plane of the culture and, thus, the intercellular relationships at different levels throughout the culture.
D000818:Animals; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D008297:Male; D008854:Microscopy, Electron / Q000379:methods; D008867:Microtomy / Q000295:instrumentation / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains; D012708:Sertoli Cells / Q000648:ultrastructure; D013737:Testis / Q000648:ultrastructure*