Investigation of immunogold-silver staining by electron microscopy.
Deposition of metallic silver on colloidal gold immunoreagents has been shown to be a very sensitive immunostaining technique capable of detecting low levels of immunoreactivity in tissue sections. Using electron microscopy we have shown that immunolabelling is highest with small sizes of gold which can penetrate sections better and achieve higher densities of particles in the section than larger particles. Chemical permeabilisation of the embedding medium aids the penetration of colloidal gold. The silver enhancement step in immunogold-silver staining was shown to be progressive, allowing optimisation of staining and the selection of the final size of silver deposits required. Some poorly understood features of the technique are rationalised and the additional knowledge gained will aid the wider application of this method.
D000818:Animals; D002531:Cerebellum / Q000648:ultrastructure; D006046:Gold; D013006:Growth Hormone / Q000378:metabolism; D008854:Microscopy, Electron / Q000379:methods*; D010903:Pituitary Gland, Anterior / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D012834:Silver; D013552:Swine
D012834:Silver; D006046:Gold; D013006:Growth Hormone