title: The biochemical identification of fibronectin in the sea urchin embryo.
abstract: We report the biochemical identification of fibronectin in the basal lamina of the sea urchin embryo. A. punctulata gastrula stage embryos were solubilized in Triton X-100 and the insoluble basal laminae extracted by incubation in buffer containing 8M urea, 2% 2-mercaptoethanol and 2% SDS. Extracted proteins were separated by SDS-PAGE, electrophoretically transferred to nitrocellulose filters and probed with monospecific antibodies directed against human plasma fibronectin (pFN). Incubation in 125I-labelled secondary antibody revealed a single band which co-migrates with human pFN at an apparent molecular weight of 220,000. This is the first direct biochemical demonstration of a fibronectin-like molecule in the sea urchin embryo which cross reacts with antibodies to vertebrate fibronectin.
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000032:analysis; D004625:Embryo, Nonmammalian / Q000032:analysis; D005353:Fibronectins / Q000276:immunology / Q000302:isolation & purification*; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D012617:Sea Urchins / Q000378:metabolism
chemical_list: D005353:Fibronectins; D011092:Polyethylene Glycols; D017830:Octoxynol