Permanent suppression of phase separation cataract in calf lens using amine modification agents.
Low temperature induced opacification (cold cataract) of the nucleus of young mammalian lenses is associated with a phase separation of proteins in the lens cell cytoplasm. Calf lenses were treated with a variety of imido-esters and N-hydroxysuccinimide-esters, which react specifically with amino groups. Many potent inhibitors of phase separation cataract were identified which lower the opacification temperature by 6 degrees C or more. Lenses generally remain clear, colorless and soft. Furthermore, suppression of the cold cataract temperature is permanent upon removal of excess reagent.
D000818:Animals; D002386:Cataract / Q000517:prevention & control*; D002417:Cattle; D003080:Cold Temperature; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D003459:Crystallins / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007096:Imidoesters / Q000494:pharmacology*; D013388:Succinimides / Q000494:pharmacology*
D003432:Cross-Linking Reagents; D003459:Crystallins; D007096:Imidoesters; D013388:Succinimides; C001426:N-hydroxysuccinimide