General method for isolation of DNA sequences that interact with specific nuclear proteins in chromosomes: binding of the high mobility group protein HMG-T to a subset of the protamine gene family.
A general method is described for the isolation of the DNA with which specific nuclear proteins interact in chromosomes. This method is based on the covalent photo-cross-linking of nuclear proteins to the DNA sequences, to which they normally bind, by means of irradiation with UV light and the selective retrieval of specific subsets of protein-DNA adducts by using specific antibodies. The application of this procedure to isolate the DNA sequences with which the trout high mobility group protein (HMG-T) interacts has shown that in trout liver this protein associates specifically with DNA sequences in proximity to a subset of the family of protamine genes but not with the histone or vitellogenin genes. From these observations, it appears that the HMG-T protein may be associated with inactive gene sequences.
D000818:Animals; D000906:Antibodies; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D002846:Chromatography, Affinity; D002875:Chromosomes / Q000378:metabolism; D004247:DNA / Q000302:isolation & purification* / Q000378:metabolism; D005796:Genes; D006609:High Mobility Group Proteins / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D009698:Nucleoproteins / Q000378:metabolism*; D011479:Protamines / Q000235:genetics*; D014337:Trout
D000906:Antibodies; D006609:High Mobility Group Proteins; D009698:Nucleoproteins; D011479:Protamines; D004247:DNA