A simple method to obtain pure granule-rich eosinophil fragments (cytosomes) from normal human blood.
This paper describes a simple, rapid and reproducible method to obtain pure granule-rich eosinophil fragments (cytosomes) with a high yield from normal human blood. The method is based on the treatment of whole blood with saponin and subsequent purification of the cytosomes on Percoll gradient. The enzymatic analysis of the cytosomes shows that the content of 3 granular enzymes is of the same order of magnitude already reported by others for intact eosinophils. This finding suggests that the cytosomes can be employed as starting material for studying the content of the granules or for the isolation of the granular components. The advantages offered by this method over those currently used to obtain eosinophil granules are discussed.
D002458:Cell Fractionation / Q000379:methods; D003593:Cytoplasm / Q000201:enzymology / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D004804:Eosinophils / Q000201:enzymology / Q000648:ultrastructure*; D006801:Humans; D008247:Lysosomes / Q000201:enzymology; D008830:Microbodies / Q000201:enzymology