Determination of monensin sodium residues in beef liver tissue by liquid chromatography of a fluorescent derivative.
Monensin sodium does not have an ultraviolet (UV) absorbance above 220 nm, and therefore cannot be detected by liquid chromatography (LC) with a UV detector. A method was developed in which monensin residues are extracted from beef liver tissue, acetylated, partitioned, and reacted with 9-anthryldiazomethane to form a fluorescent derivative for quantitation by LC. The reliable level of sensitivity is 50 ppb, but 15 ppb can be detected. Recoveries ranged between 71 and 96% with an average of 83.5%.
D000107:Acetylation; D000818:Animals; D000873:Anthracenes; D001646:Bile / Q000032:analysis; D002417:Cattle; D002853:Chromatography, Liquid; D005663:Furans / Q000032:analysis*; D008099:Liver / Q000032:analysis*; D008345:Manganese / Q000032:analysis; D017895:Manganese Compounds; D008985:Monensin / Q000032:analysis*; D010087:Oxides; D012756:Sheep; D012997:Solvents; D013050:Spectrometry, Fluorescence
D000873:Anthracenes; D005663:Furans; D017895:Manganese Compounds; D010087:Oxides; D012997:Solvents; C027347:9-diazomethylanthracene; D008345:Manganese; D008985:Monensin; C016552:manganese dioxide