title: Stabilization and gas chromatographic analysis of the four stereoisomers of 1,2,2-trimethylpropyl methylphosphonofluoridate (soman) in rat blood.
abstract: A method for the stabilization and gas chromatographic analysis of the four stereoisomers of C(+/-)P(+/-)-1,2,2-trimethylpropyl methylphosphonofluoridate (C(+/-)P(+/-)-soman) in rat blood samples is described. Satisfactory stabilization of all four stereoisomers is obtained by (i) acidification of the blood sample to pH 4.2 at 0 degrees C, to stabilize the C(+/-)P(+) isomers, (ii) addition of aluminum ions (2.5 mM) for complexation of fluoride ions, which prevents regeneration of C(+/-)P(-)-soman by free fluoride ions from soman-inhibited aliesterase, and (iii) addition of 2,2-dimethylpropyl methylphosphonofluoridate in order to occupy covalent binding sites for C(+/-)P(-)-soman. The stereoisomers of soman and internal standard are extracted from the blood-stabilizing buffer mixture with a Sep-Pak C18 cartridge and are subsequently eluted with ethyl acetate with overall extraction recoveries of 52 +/- 8%. The four soman stereoisomers are resolved and analyzed on a wide-bore capillary Chirasil Val column, synthesized, and coated in house, which also resolves the internal standard C(+/-)P(+/-)-1,2,2-[U-2H]trimethylpropyl methylphosphonofluoridate from C(+/-)P(+/-)-soman. Alternatively, the gas chromatographic analysis can be performed on a wide-bore capillary Chirasil Val column, identical with the commercially available Chirasil Val column, when combined in series with a Carbowax 20M column. This system resolves the four stereoisomers of soman and the internal standard C(-)P(+)-1,2,2-trimethylpropyl [U-2H]methylphosphonofluoridate. Using an alkali flame ionization detector, the detection limit of our procedure is ca. 250 pg soman isomer/blood sample.
mesh_terms: D000818:Animals; D002849:Chromatography, Gas / Q000379:methods; D003903:Deuterium; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007700:Kinetics; D051381:Rats; D012999:Soman / Q000097:blood* / Q000138:chemical synthesis; D013237:Stereoisomerism
chemical_list: D007202:Indicators and Reagents; D012999:Soman; D003903:Deuterium