Plasma levels and kinetic disposition of 2,4-dinitrophenol and its metabolites 2-amino-4-nitrophenol and 4-amino-2-nitrophenol in the mouse.
Eleven groups of six ICR mice were dosed orally with 22.5 mg/kg 2,4-dinitrophenol. Groups were sacrificed at 0, 0.5, 1, 2, 4, 6, 9, 12, 24, 48, and 96 h post-treatment and plasma was collected for analysis of dinitrophenol, 2-amino-4-nitrophenol, and 4-amino-2-nitrophenol content. Analyses were performed by capillary gas chromatography--mass spectrometry after liquid--liquid extraction of plasma specimens spiked with two internal standards. Quantification was based upon peak-area ratios of base peaks obtained from the three analytes and the trideuterated internal standards 2,4-dinitrophenol and 2-amino-4-nitrophenol. Plasma concentrations for each analyte versus their respective time periods were subjected to pharmacokinetic analysis. Of the two monoamine metabolites, 2-amino-4-nitrophenol was present in the greater amount and had an elimination half-life of 46 h from plasma while that of 4-amino-2-nitrophenol was 26 h.
D019297:2,4-Dinitrophenol; D000818:Animals; D003201:Computers; D004140:Dinitrophenols / Q000097:blood*; D007700:Kinetics; D051379:Mice; D008813:Mice, Inbred ICR; D008954:Models, Biological; D009596:Nitrophenols / Q000097:blood*
D004140:Dinitrophenols; D009596:Nitrophenols; C036645:2-amino-4-nitrophenol; C013399:4-amino-2-nitrophenol; D019297:2,4-Dinitrophenol