[Electron microscopic study on the structure and the function of the granulosa cell in the human ovary--in vitro electron microscopic autoradiography on the localization of 17 beta-estradiol receptor complex, DNA synthesis and protein synthesis].
In order to investigate the effect of E2 on the DNA synthesis, the localization of E2-receptor complex at different temperature, and protein synthesis in the granulosa cell of the human ovary, in vitro studies were made using the electron microscopic autoradiography of 10 muCi/ml of 3H-thymidine, 10(-8)M 3H-E2 and 100 muCi/ml of 3H-leucine. In particular, a dry-mounting autoradiographic technique was applied to detect the localization of E2-receptor complex because E2 is a soluble compound. Control incubation was done in the medium containing 10(-8)M 3H-E2 and 500-fold of unlabeled E2. In addition, as to the morphological barriers among granulosa cells, we observed the ovary treated with the 1% fixative containing 1000ppm. of ruthenium red. The labeling index was 27.6 +/- 3.9% in the medium containing E2, while it was 13.1 +/- 2.2% in the control medium (p less than 0.001). The percentage distribution of silver grains in the nucleus indicating the localization of E2-receptor was 77.4 +/- 8.6% at 37 degrees C and 25.4 +/- 9.3% at 4 degrees C respectively (p less than 0.001). The silver grains on the protein synthesis were increased in number with the passage of time and localizaed mainly on the rough endoplasmic reticulum, mitochondria and nucleus. Ruthenium red entered among the granulosa cells. From the data presented here, it is suggested that there are no morphological barriers among granulosa cells showing active protein synthesis, the nuclear transport of E2-receptor complex is dependent upon its temperature, and E2 stimulates DNA synthesis.
D000328:Adult; D001345:Autoradiography; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D008854:Microscopy, Electron; D010053:Ovary / Q000166:cytology*; D014176:Protein Biosynthesis; D011959:Receptors, Estradiol / Q000032:analysis*; D011960:Receptors, Estrogen / Q000032:analysis*
D011959:Receptors, Estradiol; D011960:Receptors, Estrogen; D004958:Estradiol; D004247:DNA