Cytochemical localization of hydrogen peroxide generating sites in the rat thyroid gland.
Sites of H2O2 generation in lightly prefixed, intact thyroid follicles were studied by two cytochemical reactions: peroxidase-dependent DAB oxidation and cerium precipitation. In both cases reaction product accumulated on the apical surface of the follicle cell at the membrane-colloid interface. The former reaction was inhibited by the peroxidase inhibitor, aminotriazole; both reactions were blocked by the presence of catalase. NADH in the medium slightly increased the amount of cerium precipitation. The ferricyanide technique for oxidoreductase activity was also applied; reaction product again was associated with the apical surface. These results strongly imply that the follicle cells have a NADH oxidizing system generating H2O2 at the apical plasma membrane.
D015100:3,3'-Diaminobenzidine; D000818:Animals; D002563:Cerium; D004121:Dimethyl Sulfoxide; D006651:Histocytochemistry; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008297:Male; D008854:Microscopy, Electron; D009243:NAD / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013961:Thyroid Gland / Q000378:metabolism* / Q000648:ultrastructure
D009243:NAD; D015100:3,3'-Diaminobenzidine; D002563:Cerium; D006861:Hydrogen Peroxide; D004121:Dimethyl Sulfoxide