Early postnatal development of calmodulin-dependent protein kinase II in rat brain.
The postnatal levels of the alpha- (Mr 50,000) and beta- (Mr 58,000/60,000) subunits of type II calmodulin-dependent protein kinase were investigated in cytosolic, cytoskeletal and nuclear subfractions of rat brain. Substantial amounts of the beta-subunit were present neonatally, especially in the cytoplasm; both subunits increased markedly during the subsequent three weeks with alpha-preponderance in the cortex and midbrain. The development of the alpha-subunit was attenuated in the cerebellum and brain stem, resulting in a relative excess of the beta-subunit. Primary neuronal cultures from fetal brain initially displayed low enzyme content, followed by an increase in the levels of the beta- but not alpha-subunit. Regulation of the cellular content of type II calmodulin-dependent protein kinase may be relevant to neuronal differentiation.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D001933:Brain Stem / Q000201:enzymology; D002147:Calmodulin / Q000494:pharmacology*; D002467:Cell Nucleus / Q000201:enzymology; D002478:Cells, Cultured; D002531:Cerebellum / Q000201:enzymology; D003599:Cytoskeleton / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D066298:In Vitro Techniques; D009474:Neurons / Q000201:enzymology; D011494:Protein Kinases / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D002147:Calmodulin; D011494:Protein Kinases