Evaluation of acid phosphatase isoenzymes in seminal fluid from normozoospermic, oligozoospermic, azoospermic and asthenoteratozoospermic men.
Acid phosphatase was measured in the semen of normozoospermic, oligozoospermic, azoospermic and asthenoteratozoospermic men: there was no significant difference between oligozoospermic and control groups whereas we observed an increase in enzyme levels in the azoospermic group and a reduction in the asthenoteratozoospermic subjects. Furthermore, an isoelectric focusing method for the separation of isoenzymes was developed. Only prostatic isoenzymes 2 and 4 were found in semen. Isoenzyme 2 sialylation profile was determined in the four groups. The sperm count did not influence this profile. These findings are discussed.
D000135:Acid Phosphatase / Q000032:analysis*; D000328:Adult; D006801:Humans; D007248:Infertility, Male / Q000201:enzymology*; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis*; D008297:Male; D009845:Oligospermia / Q000201:enzymology; D011467:Prostate / Q000201:enzymology; D012661:Semen / Q000201:enzymology*; D013076:Sperm Count
D007527:Isoenzymes; D000135:Acid Phosphatase