title: Charcoal agar, a new growth medium for the fish disease bacterium Renibacterium salmoninarum.
abstract: Charcoal is an effective replacement for serum in media for the isolation and culture of Renibacterium salmoninarum, the causative agent of bacterial kidney disease in salmonid fish. The medium, KDM-C, contains 10 g of peptone, 0.5 g of yeast extract, 1 g of L-cysteine hydrochloride, 1 g of activated charcoal, and 15 g of agar per liter and is adjusted to pH 6.8 with NaOH before autoclaving. Eight strains of R. salmoninarum grew from dilute inocula as well on KDM-C as on a standard serum-containing medium (KDM-2). The medium was effective for both primary isolations from fish and repeated transfers and has potential value for antigen preparation and physiological studies.
mesh_terms: D000362:Agar; D000818:Animals; D001424:Bacterial Infections / Q000662:veterinary*; D002606:Charcoal; D003470:Culture Media; D005393:Fish Diseases / Q000382:microbiology*; D006094:Gram-Positive Bacteria / Q000254:growth & development* / Q000302:isolation & purification; D007674:Kidney Diseases / Q000382:microbiology / Q000662:veterinary*; D012487:Salmonidae
chemical_list: D003470:Culture Media; D002606:Charcoal; D000362:Agar