Antibody to nuclear material eluted from isolated spleen vessels in systemic lupus erythematosus.
Splenic blood vessels were isolated from surrounding cells by treatment with trypsin and the use of ultrasound. This procedure retained vascular-bound immunoglobulins which were recovered by acid elution of vessels isolated from a patient with systemic lupus erythematosus. These eluted immunoglobulins reacted with material from cell nuclei as demonstrated by immunofluorescence.
D000293:Adolescent; D000328:Adult; D000974:Antibodies, Antinuclear / Q000032:analysis*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007136:Immunoglobulins / Q000032:analysis; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008856:Microscopy, Fluorescence; D013154:Spleen / Q000098:blood supply*; D005719:gamma-Globulins / Q000032:analysis*
D000974:Antibodies, Antinuclear; D007136:Immunoglobulins; D005719:gamma-Globulins