Cell sonicates used in the analysis of how measles and herpes simplex type 1 virus infections influence Vero cell mitochondrial calcium uptake.
Brief sonication was used to rapidly prepare an in situ mitochondrial Ca2+ uptake system from Vero cells. This method yielded intact, functional mitochondria capable of accumulating and retaining approximately 50% of Ca2+ available in the reaction mix. Mitochondria in cells infected with herpes simplex type 1 virus exhibited a gradual decline in the initial Ca2+ uptake rate, dropping to 65% of the control rate at the end of the 12-h lytic cycle. Influence of a lytic measles virus infection on the initial Ca2+ uptake rate was different. The rate initially dropped to 75% of the control rate by 9 h postinfection, then recovered to slightly above the control rate at 21 h postinfection, and finally declined to 75% of the control rate at 30 h postinfection. Neither viral infection altered the total amount of Ca2+ accumulated by Vero cell mitochondria.
D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D006561:Herpes Simplex / Q000378:metabolism*; D006801:Humans; D008457:Measles / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D013010:Sonication; D013997:Time Factors
D002118:Calcium