title: A three state model for alamethicin conductance in bilayer membranes.
abstract: Alamethicin is an antibiotic which produces voltage gated channels in lipid bilayer membranes. Recently completed studies of the pressure dependence of alamethicin conductance have shown that its onset following application of a suprathreshold voltage step at a pressure of 100 MPa (1000 atm) is markedly slowed relative to that observed at ambient pressure. Furthermore, the time course of the onset of conductance becomes distinctly sigmoidal at elevated pressure, a condition which is not evident at atmospheric pressure. The decay of alamethicin conductance upon removal of suprathreshold applied voltage is also slowed by application of hydrostatic pressure, but it follows a single exponential time course at all pressures. In addition, kinetic parameters characterizing the onset and decay of conductance show distinctly different pressure dependences. These observations cannot be explained by a two state model in which alamethicin moves reversibly between nonconducting and conducting states. Therefore we re-examine critically a hypothesis made by previous workers, namely that alamethicin, in monomeric or aggregate form, moves upon application of suprathreshold voltage first from a nonconducting surface state to a nonconducting preassembly or precursor state, and then finally into a conducting state. Parameters of this three state model are related to a geometric factor which measures the degree of sigmoidal conductance response and which can be evaluated directly from experimental data. An alternative aggregation-type analysis, equivalent to that applied by Hodgkin & Huxley to the potassium conductance in squid axon, is also considered in the context of this same geometric factor. The possibility of distinguishing between these analyses on the basis of experimental data is discussed.
mesh_terms: D000408:Alamethicin / Q000502:physiology*; D000900:Anti-Bacterial Agents / Q000502:physiology*; D001692:Biological Transport / Q000187:drug effects; D004553:Electric Conductivity; D007700:Kinetics; D008051:Lipid Bilayers / Q000502:physiology*; D008433:Mathematics; D008564:Membrane Potentials; D008954:Models, Biological; D011312:Pressure
chemical_list: D000900:Anti-Bacterial Agents; D008051:Lipid Bilayers; D000408:Alamethicin