An analysis of the one-stage prothrombin time.
An analysis of the one-stage prothrombin time using thromboplastins highly (Thrombotest) and slightly (Normotest) sensitive to the Pivka (protein induced by vitamin K absence) inhibitor is presented. A one-tenth dilution of blood with 0.1 mol/l Na3 citrate was satisfactory even at an extreme packed cell volume (PCV). Whole blood and plasma were equally satisfactory as test materials; however, their accuracy and precision were greatly improved when a small sample size at a high coagulation activity and a large sample size at a low coagulation activity were used. The PCV correction method which corrected for the different Pivka inhibitor sensitivities of thromboplastins was preferable.
D015415:Biomarkers; D002951:Citrates; D019343:Citric Acid; D006400:Hematocrit; D006801:Humans; D011498:Protein Precursors / Q000494:pharmacology; D011516:Prothrombin / Q000494:pharmacology; D011517:Prothrombin Time; D012015:Reference Standards; D013925:Thromboplastin / Q000037:antagonists & inhibitors; D014859:Warfarin / Q000627:therapeutic use
D015415:Biomarkers; D002951:Citrates; D011498:Protein Precursors; D019343:Citric Acid; C008208:acarboxyprothrombin; D014859:Warfarin; D011516:Prothrombin; D013925:Thromboplastin