Sensitive spectrophotometric method for the quantitative estimation of collagen.
A method to estimate collagen concentration is described. It is based on the ability of the dye Sirius Red F3BA to quantitatively precipitate collagen in acetic acid solution. Collagen content is determined by evaluating spectrophotometrically the absorbance decrease of a Sirius Red/acetic acid solution. The assay can be used in the range from 1 to 50 micrograms protein. The method is specific, sensitive, simple, and rapid.
D000085:Acetates; D001391:Azo Compounds; D011232:Chemical Precipitation; D003094:Collagen / Q000032:analysis*; D004396:Coloring Agents; D013053:Spectrophotometry / Q000379:methods
D000085:Acetates; D001391:Azo Compounds; D004396:Coloring Agents; C009798:C.I. direct red 80; D003094:Collagen