title: Critical evaluation of an indirect enzyme-linked immunosorbent assay for IgG antibodies to Dactylis glomerata.
abstract: Experiments are reported for the evaluation of an indirect ELISA using peroxidase conjugate for the detection of IgG antibodies to Dactylis glomerata (DG), a grass pollen antigen frequently responsible for allergic rhinitis and asthma. When optimized, the test proved to be useful in clinical practice for a semiquantitative, cheap and simply-performed determination of IgG antibodies to DG. The advantages and disadvantages of this method, compared with those of elaborate antigen-binding assays, are discussed.
mesh_terms: D000485:Allergens; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006801:Humans; D006967:Hypersensitivity; D007074:Immunoglobulin G / Q000032:analysis*; D007202:Indicators and Reagents; D006109:Poaceae
chemical_list: D000485:Allergens; D007074:Immunoglobulin G; D007202:Indicators and Reagents