title: Antibody to nuclear material eluted from isolated spleen vessels in systemic lupus erythematosus.
abstract: Splenic blood vessels were isolated from surrounding cells by treatment with trypsin and the use of ultrasound. This procedure retained vascular-bound immunoglobulins which were recovered by acid elution of vessels isolated from a patient with systemic lupus erythematosus. These eluted immunoglobulins reacted with material from cell nuclei as demonstrated by immunofluorescence.
mesh_terms: D000293:Adolescent; D000328:Adult; D000974:Antibodies, Antinuclear / Q000032:analysis*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007136:Immunoglobulins / Q000032:analysis; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008856:Microscopy, Fluorescence; D013154:Spleen / Q000098:blood supply*; D005719:gamma-Globulins / Q000032:analysis*
chemical_list: D000974:Antibodies, Antinuclear; D007136:Immunoglobulins; D005719:gamma-Globulins