Identification of 19-nor-corticosterone in rat urine.
19-Nor-corticosterone (19-nor-B) was isolated from a pool of urine collected from Wistar-Kyoto (WKY) rats. Identity was established by comparison of chromatographic mobilities with standard 19-nor-B both as the free compound and diacetate derivative. Comparison of the gas chromatographic-mass spectra of the urinary product and standard 19-nor-B as the diacetate following isolation by high pressure liquid chromatography (HPLC) confirmed the identity of the urinary product to be 19-nor-B. This demonstration that 19-nor-B is a naturally occurring product in addition to 19-nor-deoxycorticosterone supports the concept of the existence of a biosynthetic pathway for the formation of the 19-nor-corticosteroids.
D000107:Acetylation; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003345:Corticosterone / Q000031:analogs & derivatives* / Q000652:urine; D003900:Desoxycorticosterone / Q000031:analogs & derivatives / Q000652:urine; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D051381:Rats; D011921:Rats, Inbred WKY
D003900:Desoxycorticosterone; C019026:19-nordeoxycorticosterone; C027516:19-norcorticosterone; D003345:Corticosterone