Molecular weight distribution of proteins synthesized in single, identified neurons of Aplysia.
Parietovisceral ganglia from Aplysia californica were incubated in medium containing leucine-(3)H. Single, identified nerve cell somas were isolated from the ganglia, and their proteins extracted and separated by electrophoresis on 5% SDS-polyacrylamide gels. The distribution of total or newly synthesized proteins from the single neurons was determined by staining or slicing and liquid scintillation counting of the gels. Experiments showed that: (a) a number of proteins were being synthesized in abundance in the nerve cells; (b) different, identified neurons showed reproducibly different labeling patterns in the gels; (c) cells R2 and R15, which showed different distributions of radioactivity in the gels, had similar staining patterns; and (d) there was significant incorporation into material of high (>75,000) molecular weight in most of the cells.
D000200:Action Potentials; D000818:Animals; D004586:Electrophoresis; D005782:Gels; D007930:Leucine / Q000378:metabolism; D008970:Molecular Weight; D008974:Mollusca / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D014176:Protein Biosynthesis; D013194:Staining and Labeling; D009435:Synaptic Transmission; D014316:Tritium
D005782:Gels; D014316:Tritium; D007930:Leucine