Decreasing effects by glycyrrhizin and paeoniflorin on intracellular Ca2+-aequorin luminescence transients with or without caffeine in directly stimulated-diaphragm muscle of mouse.
Effects of glycyrrhizin (GLR) and paeoniflorin (PF), depolarizing neuromuscular blockers, on intracellular Ca2+-aequorin luminescence in the presence of or the absence of caffeine were investigated, using directly stimulated diaphragm muscle of mouse. Both GLR and PF decreased Ca2+-aequorin luminescence. The caffeine-induced increase in Ca2+-aequorin luminescence was inhibited by GLR, but not by PF. These results demonstrated that GLR and PF decreased intracellular Ca2+; GLR suppressed Ca2+-releasing from the site of action of caffeine, but PF did not.
D000331:Aequorin / Q000494:pharmacology*; D000818:Animals; D001565:Benzoates; D001952:Bridged-Ring Compounds; D002110:Caffeine / Q000494:pharmacology*; D003964:Diaphragm / Q000187:drug effects; D005960:Glucosides / Q000494:pharmacology*; D006027:Glycosides / Q000494:pharmacology*; D006034:Glycyrrhetinic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D019695:Glycyrrhizic Acid; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008164:Luminescent Proteins / Q000494:pharmacology*; D008297:Male; D051379:Mice; D039821:Monoterpenes; D009119:Muscle Contraction / Q000187:drug effects; D009132:Muscles / Q000187:drug effects*; D013390:Succinylcholine / Q000494:pharmacology
D001565:Benzoates; D001952:Bridged-Ring Compounds; D005960:Glucosides; D006027:Glycosides; D008164:Luminescent Proteins; D039821:Monoterpenes; C015423:peoniflorin; D002110:Caffeine; D000331:Aequorin; D019695:Glycyrrhizic Acid; D013390:Succinylcholine; D006034:Glycyrrhetinic Acid