Synthesis and properties of novel nucleic acid probes.
2'-O-Methyl derivatives of common ribonucleosides were prepared from appropriately protected nucleosides via the methylation of the 2'-hydroxyl groups with methyl iodide or diazomethane. These derivatives were converted into 2'-O-methylribonucleotide units and the guanosine derivative was linked to a polymer support. Thus, oligo(2'-O-methylribonucleotides) having the sequence of AmCmUmUmAmCmCmUmGm was synthesized in a stepwise manner by using the phosphotriester method. Thermal stabilities (Tm's) of the hybrid between the 2'-O-methyl ribooligomer and the complementary ribooligomer and of the related hybrids are compared. The synthesis of ribooligomer by a new approach is also described.
D001483:Base Sequence; D007202:Indicators and Reagents; D008745:Methylation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D009843:Oligoribonucleotides / Q000138:chemical synthesis*
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides