Enhanced chemiluminescent method for the detection of DNA dot-hybridization assays.
A simple enhanced chemiluminescent procedure for the quantitation of DNA hybridization to dot blots is described. The method utilizes DNA probes labeled with biotin, which are detected using a biotinylated streptavidin-horseradish peroxidase complex. The peroxidase enzyme then takes part in an enhanced chemiluminescent reaction with luminol, peroxide, and an enhancer. The method can be used to give quantitative results using a photomultiplier tube or qualitative results by recording the light emission on instant photographic film.
D001426:Bacterial Proteins; D001710:Biotin; D004247:DNA / Q000032:analysis*; D006735:Horseradish Peroxidase; D008163:Luminescent Measurements; D009693:Nucleic Acid Hybridization; D019809:Streptavidin
D001426:Bacterial Proteins; D001710:Biotin; D004247:DNA; D019809:Streptavidin; D006735:Horseradish Peroxidase