An improved perifusion system for NMR study of living cells.
Isolated cells can be kept in steady-state conditions for very long periods of time when inserted into dialysis microfibers and perifused with a nutrient-containing solution. Using this system, stable 31P NMR spectra have been obtained on dog kidney tubules for a period of 13 h.
D000818:Animals; D002478:Cells, Cultured / Q000378:metabolism*; D003470:Culture Media / Q000008:administration & dosage; D004285:Dogs; D004734:Energy Metabolism; D007684:Kidney Tubules / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation*
D003470:Culture Media