title: Ultrastructural localization of anionic phospholipids in skeletal muscle plasma membrane.
abstract: Polymyxin B was used as a probe to label anionic phospholipids in skeletal muscle plasma membrane. This antibiotic produces muscle surface membrane lesions that can be identified in both thin sections and freeze-fracture replicas. The membrane perturbations assumed a patchy distribution with a preferential localization at the level of the I band and A-I bands junction. Intramembraneous particles were also observed within the lesions. We consider the possibility that microdomains of anionic phospholipids in muscle plasma membrane may function in the binding of Ca++.
mesh_terms: D000818:Animals; D000838:Anions; D002118:Calcium / Q000378:metabolism; D002451:Cell Compartmentation; D005614:Freeze Fracturing; D008297:Male; D008854:Microscopy, Electron; D009132:Muscles / Q000648:ultrastructure*; D010743:Phospholipids / Q000378:metabolism*; D011112:Polymyxin B; D051381:Rats; D012508:Sarcolemma / Q000378:metabolism* / Q000648:ultrastructure; D012518:Sarcomeres / Q000648:ultrastructure
chemical_list: D000838:Anions; D010743:Phospholipids; D011112:Polymyxin B; D002118:Calcium