Isolation and partial characterization of high and low molecular weight acid phosphatases from chicken liver.
Two acid phosphatase forms were isolated from chicken liver by gel filtration on Sephadex G-100. These enzymes, termed I and II, have similar Km- and Vmax-values, but differ in molecular weight, optimum pH, sensitivity to various inhibitors and substrate specificity. The results were compared with the numerous literature reports of mammalian acid phosphatases.
D000135:Acid Phosphatase / Q000302:isolation & purification*; D000818:Animals; D002645:Chickens; D007527:Isoenzymes / Q000302:isolation & purification*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight
D007527:Isoenzymes; D000135:Acid Phosphatase