title: Photolytic interruptions of the bacteriorhodopsin photocycle examined by time-resolved resonance raman spectroscopy.
abstract: An investigation of the photolytic conditions used to initiate and spectroscopically monitor the bacteriorhodopsin (BR) photocycle utilizing time-resolved resonance Raman (TR3) spectroscopy has revealed and characterized two photoinduced reactions that interrupt the thermal pathway. One reaction involves the photolytic interconversion of M-412 and M', and the other involves the direct photolytic conversion of the BR-570/K-590 photostationary mixture either to M-412 and M' or to M-like intermediates within 10 ns. The photolytic threshold conditions describing both reactions have been quantitatively measured and are discussed in terms of experimental parameters.
mesh_terms: D001436:Bacteriorhodopsins / Q000378:metabolism / Q000528:radiation effects*; D002338:Carotenoids / Q000528:radiation effects*; D010782:Photolysis; D013059:Spectrum Analysis, Raman
chemical_list: D002338:Carotenoids; D001436:Bacteriorhodopsins