The M band. Studies with fluorescent antibody staining.
The M band can be extracted from fibrils suspended in 5 mM Tris buffer, pH 8.0, for 15 min. The M band is completely removed only from fibrils of sarcomere lengths greater than 2.1 micro. Extraction does not alter the fluorescent antimyosin staining pattern of the A band, thus providing strong evidence that no alteration of the structural integrity of the thick filament has occurred. Fluorescent antibody staining of the M band of unextracted fibrils can be prevented specifically by absorbing the fluorescent antibody with extracted M band material prior to staining. This verifies the specificity of the extraction procedure.
D000199:Actins; D000818:Animals; D002021:Buffers; D002645:Chickens; D004492:Edetic Acid; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D009124:Muscle Proteins / Q000302:isolation & purification*; D009210:Myofibrils / Q000032:analysis*; D009218:Myosins; D011189:Potassium Chloride; D013194:Staining and Labeling; D013997:Time Factors
D000199:Actins; D002021:Buffers; D007106:Immune Sera; D009124:Muscle Proteins; D011189:Potassium Chloride; D004492:Edetic Acid; D009218:Myosins