[DNA polymerase beta from the rat liver. Isolation, properties and inhibitory analysis of a homogeneous preparation].
A simple and reproducible purification procedure of homogeneous DNA polymerase beta from rat liver is developed, including sedimentation and saline extraction of rat liver chromatin, chromatography of the extract on DEAE-cellulose, phosphocellulose, Gel Blue A, and DNA sepharose. The purified enzyme isolated with the 8.4% yield proved to be a homogeneous protein with m.w. 38-40 kDa, specific activity 31 units/g, pI 8.6-8.9. Incorporation of [3H]TTP into activated DNA catalysed by DNA polymerase beta was strongly inhibited by dNTP (3'NH2), ddTTP, dNTP (3'F) and slightly inhibited by aCTP and aNTP (3'NH2).
D000818:Animals; D002843:Chromatin / Q000032:analysis; D002847:Chromatography, Agarose; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004259:DNA-Directed DNA Polymerase / Q000032:analysis / Q000302:isolation & purification*; D003854:Deoxyribonucleotides / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D019384:Nucleic Acid Synthesis Inhibitors; D011489:Protein Denaturation; D051381:Rats
D002843:Chromatin; D003854:Deoxyribonucleotides; D019384:Nucleic Acid Synthesis Inhibitors; D004259:DNA-Directed DNA Polymerase
DNK-polimeraza beta iz pecheni krysy. Poluchenie, svoĭstva i ingibitornyĭ analiz gomogennogo preparata.