Isolation and quantitative analysis of phosphatidylglycerol and glycolipid molecular species using reversed-phase high-performance liquid chromatography with flame ionization detection.
Conditions are described for the quantitative analysis of phosphatidylglycerol and plant glycolipid molecular species by reversed-phase high-performance liquid chromatography employing a commercially available flame ionization detector. Direct detection on a mass basis overcomes the problem of poor detectability found with most natural lipids. Effective mobile phases composed primarily of volatile solvents are described. Splitting of the column eluate stream allows a portion of each individual molecular species to be recovered for other types of analysis.
D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D005410:Flame Ionization; D006017:Glycolipids / Q000032:analysis*; D010715:Phosphatidylglycerols / Q000032:analysis*
D006017:Glycolipids; D010715:Phosphatidylglycerols