The binding of 9-aminoacridine to calf thymus DNA in aqueous solution. Electronic spectral studies.
Absorption, circular dichroism and steady-state fluorescence spectra were determined of 9-aminoacridine solutions in the presence of DNA at an ionic strength of 0.001 mol dm-3. Up to a dye/DNA phosphorus ratio of about 0.2 the results are fully consistent with the requirements and predictions of a binding model already shown to apply to the binding of other aminoacridines to DNA. The apparently anomalous spectroscopic behaviour of the 9-aminoacridine/DNA system compared with proflavine/DNA, for example, can be satisfactorily explained from a consideration of the magnitudes of exciton interactions between dyes bound to DNA.
D000585:Aminacrine; D000609:Aminoacridines; D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA; D009690:Nucleic Acid Conformation; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013950:Thymus Gland
D000609:Aminoacridines; D012996:Solutions; D000585:Aminacrine; D004247:DNA