Cytotoxicity of rabbit macrophage peptides MCP-1 and MCP-2 for mouse tumor cells.
The cytotoxicity of cationic peptides MCP-1 and MCP-2 isolated from rabbit alveolar macrophages was tested against two tumor cell lines of murine lymphosarcoma origin, RAW117-P and RAW117-H10, and a normal mouse connective tissue fibroblast strain, ATCC CCL1.RAW117-H10 is a highly malignant metastatic variant derived from the less malignant RAW117-P. Our results indicate that these peptides possess a cytotoxic activity against the tumor cells tested but not against the normal cells tested. At concentrations of 30 micrograms/ml, these peptides completely killed RAW117-H10 cells in suspension cultures, while inhibiting growth of RAW117-P for but a limited period of time, up to 48 h, after which growth resumed. RAW117-P cells were killed by concentrations of 40 micrograms/ml. These peptides showed little cytotoxicity for normal mouse connective tissue fibroblasts at concentrations of 40 micrograms/ml.
D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D004355:Drug Stability; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000473:pathology*; D011506:Proteins / Q000032:analysis / Q000494:pharmacology*; D011817:Rabbits
D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D011506:Proteins