[Analysis of cellular nucleoproteins by the nucleoprotein-celite chromatography method. III. Two types of DNA-nuclear matrix interaction].
There are two types of DNA-nuclear matrix interactions in animal cells as revealed by the release of DNA from isolated nuclei by three successive gradients: NaCl, LiCl-urea and temperature. Nuclei were treated with dissociating agents while being adsorbed on the Celite columns. "Weak" DNA-matrix interactions which dissociate in 1.5 M LiCl-3 M urea at 2 degrees appear to be sensitive to ethidium bromide and resistant to exogeneous nucleases (DNAase I, DNAase II and micrococcal nuclease), to DNA-damaging agents, including alkylators and gamma-irradiation, and also to psoralen-induced cross-links. "Strong" DNA-matrix interactions proved to be very different. They dissociate in 4 M LiCl-8 M urea at approximately 90 degrees, are very sensitive to DNAase I and other nucleases, slightly sensitive to chemicals and irradiation at doses stimulating single-stranded DNA breaks, but resistant to ethidium bromide. DNA strand separation seems to be necessary prerequisite for DNA release from its "strong" complex with nuclear matrix. A model for the topological link between DNA and the nuclear matrix involved in DNA replication complex is discussed.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D002478:Cells, Cultured; D002845:Chromatography; D004247:DNA / Q000302:isolation & purification* / Q000528:radiation effects; D004273:DNA, Neoplasm / Q000302:isolation & purification; D007692:Diatomaceous Earth; D005347:Fibroblasts / Q000032:analysis; D006367:HeLa Cells / Q000032:analysis; D006801:Humans; D006868:Hydrolysis; D008114:Liver Neoplasms, Experimental / Q000032:analysis; D008730:Methoxsalen / Q000494:pharmacology; D051379:Mice; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009698:Nucleoproteins / Q000302:isolation & purification*; D051381:Rats
D004273:DNA, Neoplasm; D009698:Nucleoproteins; D007692:Diatomaceous Earth; D004247:DNA; D008730:Methoxsalen
Analiz kletochnykh nukleoproteidov metodom nukleoproteid-tselit-khromatografii. III. Dva tipa vzaimodeĭstviĭ DNK s iadernym matriksom.