title: Effects of ionic strength and sulfhydryl reagents on the binding of creatine phosphokinase to heart mitochondrial inner membranes.
abstract: The concept that creatine phosphokinase is bound to the outer surface of the heart mitochondrial inner membrane originated from observations that the enzyme is retained by water-swollen heart mitochondria and by digitonin-treated heart mitochondria suspended in isotonic sucrose. The present study establishes that digitonin-treated mitochondria release creatine phosphokinase in isotonic KCl, and other investigators have reported an identical response for the water-swollen organelles. These observations suggest that mitochondrial creatine phosphokinase is not bound to the outer surface of the inner membrane at a site adjacent to the adenine nucleotide translocase under physiologic conditions.
mesh_terms: D000263:Adenylate Kinase / Q000378:metabolism; D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D002021:Buffers; D002417:Cattle; D003402:Creatine Kinase / Q000378:metabolism*; D004072:Digitonin / Q000494:pharmacology; D006902:Hydroxymercuribenzoates / Q000494:pharmacology; D007425:Intracellular Membranes / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D008929:Mitochondria, Heart / Q000201:enzymology* / Q000378:metabolism; D009994:Osmolar Concentration; D011189:Potassium Chloride / Q000378:metabolism; D013439:Sulfhydryl Reagents / Q000494:pharmacology*
chemical_list: D002021:Buffers; D006902:Hydroxymercuribenzoates; D013439:Sulfhydryl Reagents; D011189:Potassium Chloride; D003402:Creatine Kinase; D000263:Adenylate Kinase; D004072:Digitonin