Histochemical staining of exogenous chromium and iron with the catalytic oxidation of phenylamines.
A highly sensitive and specific method for staining exogenous chromium and iron in tissues is described. This method is superior to conventional complex-forming methods with regard to its sensitivity and specificity for these metals. The staining reaction is based on the metal-catalysed oxidation of phenylamines. Tissue sections were incubated in a medium containing hydrogen peroxide and phenylamines, p-phenylenediamine or phenylhydrazine. Results obtained from test-tube experiments concerning the catalytic activities of metals indicated that the staining reactions depends on the activities of metals in tissues.
D000814:Aniline Compounds; D000818:Animals; D002384:Catalysis; D002857:Chromium; D006651:Histocytochemistry; D007501:Iron; D008099:Liver / Q000032:analysis; D010084:Oxidation-Reduction; D051381:Rats
D000814:Aniline Compounds; D002857:Chromium; D007501:Iron