title: Different conformations of ribosomal DNA in active and inactive chromatin in Xenopus laevis.
abstract: The chromatin structure of the ribosomal DNA in Xenopus laevis was studied by micrococcal nuclease digestions of blood, liver and embryonic cell nuclei. We have found that BglI-restricted DNA from micrococcal nuclease-digested blood cell nuclei has an increased electrophoretic mobility compared to the undigested control. Micrococcal nuclease digestion of liver cell nuclei causes a very slight shift in mobility, only in the region of the spacer containing the "Bam Islands". In contrast, the mobility of ribosomal DNA in chromatin of embryonic cells, under identical digestion conditions, remains unaffected by the nuclease activity. Denaturing gels or ligase action on the nuclease-treated DNA abolishes the differences in the electrophoretic mobility. Ionic strength and ethidium bromide influence the relative electrophoretic migration of the two DNA fragment populations, suggesting that secondary structure may play an important role in the observed phenomena. In addition, restriction analysis under native electrophoretic conditions of DNA prepared from blood, liver and embryonic cells shows that blood cell DNA restriction fragments always have a faster mobility than the corresponding fragments of liver and embryo cell DNA. We therefore propose that nicking activity by micrococcal nuclease modifies the electrophoretic mobility of an unusual DNA conformation, present in blood cell, and to a lesser extent, in liver cell ribosomal chromatin. A possible function for these structures is discussed. The differences of the ribosomal chromatin structures in adult and embryonic tissues may reflect the potential of the genes to be expressed.
mesh_terms: D000818:Animals; D002843:Chromatin; D004275:DNA, Ribosomal; D003850:Deoxyribonuclease I; D004587:Electrophoresis, Agar Gel; D004625:Embryo, Nonmammalian / Q000032:analysis; D004706:Endodeoxyribonucleases; D004996:Ethidium; D008099:Liver / Q000032:analysis; D008836:Micrococcal Nuclease; D008854:Microscopy, Electron; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D009994:Osmolar Concentration; D012335:RNA, Ribosomal; D014982:Xenopus laevis / Q000235:genetics
chemical_list: D002843:Chromatin; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D004706:Endodeoxyribonucleases; D003850:Deoxyribonuclease I; C019329:deoxyribonuclease II; D008836:Micrococcal Nuclease; D004996:Ethidium