Effect of lipoproteins and luteotrophins on progesterone accumulation by luteal cells from the pregnant pig.
The roles of prolactin (Prl) and LH in the maintenance of luteal function in pregnant pigs were investigated. Luteal cells from pigs between days 70 to 95 of pregnancy were dissociated and incubated for 4 h. In the absence of exogenous cholesterol, LH exhibited a dose-dependent stimulatory effect on progesterone secretion. Prl had a mild stimulatory effect on progesterone accumulation and at lower doses Prl potentiated the response to LH. Low density lipoprotein (LDL) but not high density lipoprotein (HDL) had a mild stimulatory effect on progesterone secretion. When exogenous cholesterol was provided as the substrate in the form of LDL or HDL, Prl had a striking stimulatory effect on progesterone secretion. When 25-hydroxycholesterol which bypasses the lipoprotein receptor was provided as the substrate, Prl failed to stimulate progesterone accumulation. The stimulatory effect of LH was potentiated when LDL, HDL, or 25-hydroxycholesterol were present. The results of this study suggest that LH increases the uptake of exogenous cholesterol in the form of lipoproteins and enhances the utilization of internalized cholesterol for progesterone synthesis. Prl appears to stimulate progesterone synthesis by enhancing the uptake of lipoproteins.
D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D003338:Corpus Luteum / Q000378:metabolism*; D005260:Female; D006888:Hydroxycholesterols / Q000494:pharmacology; D066298:In Vitro Techniques; D008074:Lipoproteins / Q000494:pharmacology*; D008075:Lipoproteins, HDL / Q000494:pharmacology; D008077:Lipoproteins, LDL / Q000494:pharmacology; D008184:Luteal Cells / Q000187:drug effects / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000494:pharmacology*; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism*; D011388:Prolactin / Q000494:pharmacology*; D011863:Radioimmunoassay; D013552:Swine
D006888:Hydroxycholesterols; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D011374:Progesterone; C007997:25-hydroxycholesterol; D011388:Prolactin; D007986:Luteinizing Hormone; D002784:Cholesterol