[Methodological variants in the optimal determination of the activity of natural killers and mononuclear phagocytes by analysis at the single-cell level].
Variants of the method for determination of the natural killer cells (NK-cells), and monocytes-macrophages both in human beings and in mice have been described. Usage of agarose which permits fixing conjugates of the effector cells and a tumour target cell allows determining simultaneously the quantity of effector cells bound with the target and percentage of the cytotoxic effectors. The results in acceptable definition of the effector cells' activity with the use of various kinds of plastic ware, its treatment, diverse types of agarose, temperature and time regimen are adduced. The di-target method of testing is approved using the target cells labelled with fluorescein as well as unlabelled ones. Peculiarities of function disturbances of the NK-cells and monocytes in patients with lung cancer as well as changes in the activity of these effectors in mice CBA depending on the terms of Krebs-2 carcinoma inoculation and on repeated BCG vaccine injection were detected.
D000818:Animals; D001782:Blood Donors; D002460:Cell Line; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic / Q000295:instrumentation / Q000379:methods; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D008175:Lung Neoplasms / Q000276:immunology; D051379:Mice; D008808:Mice, Inbred CBA; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology; D010586:Phagocytes / Q000276:immunology*; D013696:Temperature; D013997:Time Factors
Metodicheskie varianty optimal'nogo opredeleniia aktivnosti estestvennykh killerov i mononuklearnykh fagotsitov s analizom na urovne odnoĭ kletki.