Influence of a low molecular weight serine proteinase inhibitor (camostate) on lipolysis of isolated adipocytes.
The effect of the epsilon-guanidino acid derivate camostate (FOY 305) on lipolysis of isolated adipocytes was evaluated to test its supposed influence on the reactions of lipid metabolism in experimental shock. Isoprenaline (10(-6) M) and theophylline (10(-3) M) significantly stimulated lipolysis in vitro. The basal submaximal (isoprenaline 10(-6)-10(-9) M) and maximal (isoprenaline 10(-6) M) catecholamine-stimulated lipolysis were not affected by camostate (10(-6) M). Furthermore, camostate (0.1-100 X 10(-6) M) did not influence half-maximal theophylline-induced lipolysis of the adipocytes. Our findings do not support the suggestion that epsilon-guanidino acid derivatives diminish in shock the increase of free fatty acids in blood via an influence on lipolysis in the peripheral adipose tissue.
D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D004952:Esters; D016670:Gabexate / Q000031:analogs & derivatives*; D006146:Guanidines / Q000494:pharmacology*; D008066:Lipolysis / Q000187:drug effects*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012769:Shock / Q000188:drug therapy
D004952:Esters; D006146:Guanidines; C034532:camostat; D016670:Gabexate