A simplified procedure for preparation of undecalcified human bone sections.
A new type of apparatus for sectioning samples of hard, undecalcified bone is described. Slices of fresh or archeological human bone 4-5 mm thick are dehydrated and then embedded in epoxy resin. The apparatus used to prepare sections from the resulting blocks consists of a low-speed rim-type diamond cut-off wheel and a slowly advancing table carrying the specimen held in a rotating mount. Sections may be cut at a thickness of 80 micron +/- 1%. After cleaning in an ultrasonic bath, these can be mounted on slides for quantitative microscopic examination with transmitted light. Grinding and polishing are not necessary. The results obtained are illustrated.
D001842:Bone and Bones / Q000166:cytology*; D002113:Calcification, Physiologic; D004853:Epoxy Resins; D005269:Femur; D005404:Fixatives; D005580:Fossils; D006801:Humans; D008867:Microtomy / Q000295:instrumentation / Q000379:methods*
D004853:Epoxy Resins; D005404:Fixatives