Growth of Aeromonas spp. on cefsulodin-Irgasan-novobiocin agar selective for Yersinia enterocolitica.
Twenty-eight strains of Aeromonas spp. were analyzed for their ability to grow on two different kinds of cefsulodin-Irgasan (triclosan; Ciba-Geigy AG, Basel, Switzerland)-novobiocin (CIN) agar containing 15 or 4 mg of cefsulodin per ml and on inositol-bile salts-brilliant green (IBB) agar. Relative to blood agar, 68% of the strains were inhibited by more than 2 logs (i.e., less than 1% growth) at 37 degrees C (39% at 25 degrees C) on CIN I (high cefsulodin concentration), 7% were inhibited at either temperature on CIN II (low cefsulodin concentration), 4% were inhibited on IBB agar at 37 degrees C, and none were inhibited on IBB agar at 25 degrees C. These results reflect the MICs of cefsulodin on CIN Base: the MIC for 50% of the strains was 8 mg/liter at 37 and 25 degrees C, and the MICs for 90% of the strains were 16 mg/liter at 37 degrees C and 64 mg/liter at 25 degrees C. The MICs of Irgasan and novobiocin were far beyond the concentrations used in CIN media. We argue that CIN agar containing 4 mg of cefsulodin per ml (CIN II) can be used for the simultaneous detection of Aeromonas spp. and Yersinia spp.
D000333:Aeromonas / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification; D002224:Carbanilides / Q000494:pharmacology; D002441:Cefsulodin / Q000494:pharmacology; D003470:Culture Media; D005243:Feces / Q000382:microbiology; D006801:Humans; D009675:Novobiocin / Q000494:pharmacology; D013696:Temperature; D015008:Yersinia enterocolitica / Q000254:growth & development
D002224:Carbanilides; D003470:Culture Media; D009675:Novobiocin; C005055:cloflucarban; D002441:Cefsulodin