Some aspects of the glycoprotein and glycopolypeptide content of human gastric mucus.
The carbohydrate content of non-diffusable, glycoprotein and glycopolypeptide material has been studied in normal human gastric aspirates. Pentagastrin doubles the volume of secretions but has no effect on the amount of non-diffusable material. Only about 40% of the weight of the non-diffusable material is mucin in nature. Gel-permeation chromatography indicated that about half of the mucin survived as high molecular mass glycoprotein. Monosaccharide differences, for example between secretors and non-secretors, only became manifest at the glycopolypeptide stage. These results emphasize the dangers of attempting to assess mucin changes by simple carbohydrate analyses of unfractionated gastric aspirates.
D000293:Adolescent; D000328:Adult; D002241:Carbohydrates / Q000032:analysis; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism*; D006020:Glycopeptides / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D008297:Male; D009093:Mucus / Q000032:analysis*; D010418:Pentagastrin; D013396:Suction
D002241:Carbohydrates; D006020:Glycopeptides; D006023:Glycoproteins; D010418:Pentagastrin