Purification of peptides derived from proenkephalin A on Bio-Rex 70.
The weakly acidic, cation-exchange resin Bio-Rex 70 was used at a low pH for chromatography of enkephalins and other peptides containing enkephalin sequences. Recoveries of various peptide standards and peptides in adrenal chromaffin cell extracts were greater than 85%. Catecholamines and other substances such as density gradient media are not retained by the resin under the conditions employed. A significant purification of enkephalins and larger peptides containing enkephalin sequences with respect to total protein was also obtained by this method. This chromatographic technique should be useful where a low-cost, preliminary purification of samples containing peptides derived from proenkephalin A is required and may be adaptable to other peptides as well.
D000313:Adrenal Medulla / Q000032:analysis*; D000818:Animals; D002411:Cation Exchange Resins; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004723:Endorphins / Q000302:isolation & purification*; D004745:Enkephalins / Q000302:isolation & purification*; D007475:Ion Exchange Resins; D011498:Protein Precursors / Q000302:isolation & purification*; D012117:Resins, Synthetic
D002411:Cation Exchange Resins; D004723:Endorphins; D004745:Enkephalins; D007475:Ion Exchange Resins; D011498:Protein Precursors; D012117:Resins, Synthetic; C029992:proenkephalin; C026257:Bio-Rex 70