[Flow cytometric measurements of in vivo incorporated bromodeoxy- uridine into the DNA of experimental and human tumors].
A new cell kinetic method was used in vivo in an experimental mouse tumour and in human tumours of the oral cavity for the first time. The incorporation of BUdR into cells in the S-phase that are actively synthesizing DNA enables the determination of the rate of DNA synthesis from the amount of BUdR-related fluorescence using monoclonal antibody and the proportion of S-phase cells which are actively engaged in synthesis. The application of BUdR to the patients and the preparation of the tumour is reported.
D000230:Adenocarcinoma / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D005434:Flow Cytometry; D006801:Humans; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism
D000911:Antibodies, Monoclonal; D004273:DNA, Neoplasm; D001973:Bromodeoxyuridine
Flusszytometrische Messungen von in vivo inkorporiertem Bromodeoxyuridin (BUdR) in die DNA von experimentellen und menschlichen Tumoren.