Evaluation of a new procedure for isoamylase measurement by selective inhibition.
The authors have evaluated a new procedure for the estimation of pancreatic and salivary isoamylase using a new selective inhibitor reagent (Enzamyl Isoamylase, Gödecke), Germany; composition covered by patent No. P 3404876.6). Provided the conditions of the assay presented here are correctly selected, a standard curve is not necessary and the activities of both isoenzymes can be calculated by a simple formula. In view of its rapidity and precision, this new procedure appears to be useful as a screening test. It correlates closely with a reference electrophoretic method.
D004386:Duodenum / Q000201:enzymology; D005069:Evaluation Studies as Topic; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006801:Humans; D007202:Indicators and Reagents; D007419:Intestinal Secretions / Q000201:enzymology; D007517:Isoamylase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008722:Methods; D010179:Pancreas / Q000201:enzymology*; D012463:Saliva / Q000201:enzymology
D007202:Indicators and Reagents; D006026:Glycoside Hydrolases; D007517:Isoamylase