Isolation and amino acid sequence of a short-chain neurotoxin from an Australian elapid snake, Pseudechis australis.
A short-chain neurotoxin Pseudechis australis a (toxin Pa a) was isolated from the venom of an Australian elapid snake Pseudechis australis (king brown snake) by sequential chromatography on CM-cellulose, Sephadex G-50 and CM-cellulose columns. Toxin Pa a has an LD50 (intravenous) value of 76 micrograms/kg body wt. in mice and consists of 62 amino acid residues. The amino acid sequence of Pa a shows considerable homology with those of short-chain neurotoxins of elapid snakes, especially of true sea snakes.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002852:Chromatography, Ion Exchange; D002918:Chymotrypsin; D004546:Elapid Venoms / Q000032:analysis*; D009498:Neurotoxins / Q000302:isolation & purification; D010446:Peptide Fragments / Q000032:analysis
D000596:Amino Acids; D004546:Elapid Venoms; D009498:Neurotoxins; D010446:Peptide Fragments; D002918:Chymotrypsin