title: [Mechanism of action of cAMP-dependent protein kinase. V. Free energy spectra].
abstract: The catalytic subunit of cAMP-dependent protein kinase from pig brain was shown to catalyse an isotope exchange reaction ATP in equilibrium with ADP. The kinetic parameters of the exchange were determined. The enzyme can also use GTP as the donor substrate; phosphotransferase and "GTPase" reactions were investigated. Based on the kinetic data obtained in this and in the previous paper the free energy profiles of protein kinase catalysed reactions are discussed.
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D002384:Catalysis; D004734:Energy Metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008954:Models, Biological; D010766:Phosphorylation; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D047428:Protein Kinase Inhibitors; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D011494:Protein Kinases
vernacular_title: Mekhanizm deĭstviia cAMP-zavisimoĭ proteinkinazy. V. Profili svobodnoĭ énergii.