Investigations on the messenger RNA binding site of eukaryotic ribosomes by using reactive oligo(U) derivatives.
Dodecauridylate bearing a slowly alkylating reactive group in 5'-position, when bound as a mRNA analogue to rat liver ribosomes, labels preferentially proteins of the small ribosomal subunit, in particular, protein S 26.fDodecauridylate bearing an identical reactive group in 3'-position labels mainly rRNA o both subunits and proteins to less than 10%, among which S3/3a are the main targets. The results together with earlier findings support the localization of protein S 26 near the ribosomal P site and S 3/3a near the A site as well as the importance of rRNA of both subunits for interaction with mRNA when it passes the region in front of the decoding site.
D000818:Animals; D001665:Binding Sites; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D009843:Oligoribonucleotides / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism; D051381:Rats; D012269:Ribosomal Proteins / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014500:Uracil Nucleotides / Q000378:metabolism*
D009843:Oligoribonucleotides; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D014500:Uracil Nucleotides; C027916:oligo(U)