Proximity of the Mep-1 gene to H-2D on chromosome 17 in mice.
The Mep-1 gene on chromosome 17 in mice controls the activity of meprin, a kidney brush border metalloendopeptidase. Most inbred mouse strains of the k haplotype (e.g., CBA, C3H, AKR) are markedly deficient in meprin activity; these mice carry the Mep-1b allele. Mouse strains in which meprin activity levels are normal are designated Mep-1a. Studies using congenic and recombinant strains mapped the Mep-1 gene telomeric to H-2D near the Tla gene. To further study the relationship between the major histocompatibility complex and Mep-1, a linkage study was conducted. Mep-1a F1 hybrids [C3H.A (KkDd) X C3H.OH (KdDk)] were backcrossed with Mep-1b C3H.OH (KdDk) parents. The progeny were assayed for H-2D markers, Pgk-2 isozymes, and meprin activity. Recombination between H-2D and Mep-1 occurred in 6 out of 284 mice, a crossover frequency of 2.1%. Mep-1 is therefore 2.1 crossover units telomeric to H-2D and approximately 0.6 crossover units from Tla. The Mep-1 locus provides a new genetic marker for the future mapping of this important area of the mouse genome.
D000603:Amino Acids, Sulfur / Q000235:genetics*; D000818:Animals; D002874:Chromosome Mapping; D005796:Genes; D008040:Genetic Linkage; D006183:H-2 Antigens / Q000235:genetics*; D008285:Major Histocompatibility Complex; D051379:Mice / Q000235:genetics* / Q000276:immunology; D010641:Phenotype; D010735:Phosphoglycerate Kinase / Q000235:genetics; D008625:Tiopronin / Q000235:genetics*
D000603:Amino Acids, Sulfur; D006183:H-2 Antigens; D008625:Tiopronin; D010735:Phosphoglycerate Kinase