title: Effect of the time of extraction on the potency and allergenic composition of the extract of Parietaria officinalis pollen.
abstract: Five Parietaria officinalis pollen extracts were obtained after respective 0.5, 4, 16, 48, 96 h of extraction in 0.0125 M NH4HCO3 pH 8.7 containing 0.05% NaN3 and 5.7 X 10(-4) M phenylmethylsulphonylfluoride. Conductivity, pH, absorption coefficient at 280 and 360 nm and content in protein and carbohydrates were determined in the extracts. Allergenic potency, evaluated by RAST inhibition was higher for the shorter-time extracts. The allergenic composition of the extracts was analyzed by a combination of gel filtration of the extracts and RAST inhibition of the eluted fractions: low molecular weight allergens were recovered only in longer-time extracts.
mesh_terms: D008722:Methods; D008970:Molecular Weight; D010860:Pigments, Biological / Q000032:analysis; D011058:Pollen / Q000032:analysis / Q000276:immunology; D013997:Time Factors
chemical_list: D010860:Pigments, Biological