Purification and radioimmunoassays for superoxide dismutases in the mouse: tissue concentrations in different strains.
Both murine cuprozinc and manganosuperoxide dismutases were purified to electrophoretic homogeneity from liver; the former (dimer) had a sp. act. of 2600 U/mg and a subunit mol. wt. of 17,000, the latter (tetramer) 2300 U/mg and mol. wt. 23,000. Heterologous radioimmunoassays had sensitivities of 3.1 ng/ml for the former enzyme and 2.5 ng/ml for the latter, and were appicable across murine genetic lines (Balb/c, ob/ob, db/db). Islets had the lowest concentrations of both enzymes among the tissues studied, and this could explain their vulnerability to free-radical damage.
D000818:Animals; D008099:Liver / Q000201:enzymology; D051379:Mice; D008817:Mice, Mutant Strains; D008970:Molecular Weight; D011863:Radioimmunoassay; D013482:Superoxide Dismutase / Q000276:immunology* / Q000302:isolation & purification*
D013482:Superoxide Dismutase