Effect of alkyltins on rabbit articular and growth-plate chondrocytes in monolayer culture.
The effect of four different alkyltins (trimethyltin, triethyltin, dibutyltin, and dioctyltin) on the metabolism of rabbit articular and growth-plate chondrocytes was investigated using a monolayer cell-culture system. In most instances the compounds tested exhibited a general cytotoxic effect on these cells, inhibiting the synthesis of both DNA and sulfated proteoglycans. The effect of these compounds on proteoglycan synthesis was both quantitative and qualitative, as demonstrated by CsCl isopycnic density gradient centrifugation and gel exclusion chromatographic techniques. However, certain tin compounds tested, at specific concentrations, exerted a stimulatory effect on chondrocyte proliferation. Regarding DNA synthesis, growth-plate chondrocytes were more sensitive to the effect of the triethyltin, dibutyltin, and dioctyltin than were articular chondrocytes. The data are discussed in relation to the possible effects of the alkyltins on skeletal growth and development as well as the mechanism of action of the alkyltins at the molecular level.
D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects*; D002478:Cells, Cultured; D002500:Centrifugation, Isopycnic; D004261:DNA Replication / Q000187:drug effects; D004838:Epiphyses / Q000166:cytology / Q000187:drug effects*; D005260:Female; D006025:Glycosaminoglycans / Q000378:metabolism; D009947:Organotin Compounds / Q000494:pharmacology*; D011509:Proteoglycans / Q000378:metabolism; D011817:Rabbits; D013431:Sulfates / Q000378:metabolism; D014220:Trialkyltin Compounds / Q000494:pharmacology*; D014267:Triethyltin Compounds / Q000494:pharmacology*; D014298:Trimethyltin Compounds / Q000494:pharmacology*
D006025:Glycosaminoglycans; D009947:Organotin Compounds; D011509:Proteoglycans; D013431:Sulfates; D014220:Trialkyltin Compounds; D014267:Triethyltin Compounds; D014298:Trimethyltin Compounds; C046488:trimethyltin; C031575:triethyltin; C020735:dibutyldichlorotin; C020297:di-n-octyltin dichloride