Purification of an onco-fetal antigen from mammary carcinoma cells.
Mammary carcinoma cell membranes were solubilized by a zwitterionic detergent, 3-(3-cholamidopropyl)-dimethyl-ammonio)-propanesulfonate (CHAPS). The solubilized membrane components were resolved by high pressure liquid chromatography (HPLC) using a styrogel protein column. One of the fractions was found to react specifically with anti-F9-antiserum in a solid-phase immunosorbent assay. Following recycling this fraction was purified to homogeneity and had an N-terminal amino acid residue of lysine. Its molecular weight was estimated to be 15,000 daltons. No comparable fraction could be detected in the solubilized membrane components of nonmalignant mammary tissue.
D000951:Antigens, Neoplasm / Q000032:analysis*; D001943:Breast Neoplasms / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology; D005260:Female; D006801:Humans; D008970:Molecular Weight
D000951:Antigens, Neoplasm