[Action of pro-oxidant diets on the enzymatic system of Ca2+ transport in the sarcoplasmic reticulum].
August male rats were kept for 90 days on one of the following diets: balanced semisynthetic diet with casein as a source of protein (group 1), amino acid balanced diet with casein replaced by gluten (group 2), a diet with excess of polyunsaturated fatty acids (group 3), with vitamin E deficiency (group 4), and polyunbalanced diet, comprising a combination of amino acid imbalance, excess of polyunsaturated acids, and vitamin E deficiency (group 5). Structural and functional parameters of Ca2+ transport (Ca2+ accumulation rate, activity of Ca2+-ATPase, Ca/ATP ratio), content of lipid fractions and accumulation of malonic dialdehyde were studied in sarcoplasmatic reticulum (SR) fragments from rat hind limb muscles in the course of ascorbate-dependent lipid peroxidation. Reduction of Ca2+ absorption rate, Ca2+-ATPase, and Ca/ATP ratio in SR membranes were observed in groups 2-5. In parallel, decreased phospholipid and triglyceride levels and increased content of free fatty acids and cholesterol in SR membranes were established.
D000596:Amino Acids / Q000172:deficiency; D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D004032:Diet; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*; D014811:Vitamin E Deficiency / Q000201:enzymology
D000596:Amino Acids; D005231:Fatty Acids, Unsaturated; D008563:Membrane Lipids; D008565:Membrane Proteins; D002118:Calcium
Deĭstvie prooksidantnykh ratsionov na fermentnuiu sistemu transporta Ca2+ v sarkoplazmaticheskom retikulume.