Alu sequences transcription in X. laevis oocytes: nuclear-cytoplasmic partitioning and evidence for 3' end processing reactions.
A large Alu-family cluster in the 5' flanking region of a human alpha 1-acid glycoprotein gene has been identified and sequenced. Individual members microinjected into X. laevis oocytes are transcribed only when canonical box A and B components of the split pol III promoter are present. Alu transcripts accumulate in the nucleus. An unusually short Alu transcript, able to assume a stable secondary structure, undergoes a 3' end processing reaction similar to the one required for tRNA 3' end maturation.
D000818:Animals; D001483:Base Sequence; D002451:Cell Compartmentation; D002467:Cell Nucleus / Q000502:physiology; D003593:Cytoplasm / Q000502:physiology; D008040:Genetic Linkage; D006801:Humans; D009690:Nucleic Acid Conformation; D009961:Orosomucoid / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic; D014982:Xenopus laevis / Q000235:genetics
D009961:Orosomucoid