title: Choline acetyltransferase and acetylcholinesterase in canine spinal ganglia: increase of choline acetyltransferase activity following sciatic nerve lesion.
abstract: Activities of choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) were measured in the dorsal spinal ganglia, the dorsal spinal root and the spinal cord of the normal adult dogs and following one side transection of the sciatic nerve in the intervals 5, 10, 15 and 21 days respectively. In the spinal ganglia of normal dogs very low ChAT activity was found; it was three orders lower than AChE activity. Within 5-10 days after the nerve section ChAT activity increased almost five times in the spinal ganglia while AChE activity remained without any changes. The elevation of ChAT activity correlated with that in the dorsal roots at 15th day and in the dorsal spinal cord at 21st day after the nerve section. Histochemical "direct-colouring" thiocholine method showed AChE-positive cells were distributed mainly in the peripheral area of the spinal ganglia. The spinal ganglion cells ranged from intensely AChE-positive to AChE-negative without correlation between cell size and AChE activity. The ChAT activity changes were evaluated in correlation to the cholinergic function in the spinal ganglion neurons.
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000344:Afferent Pathways / Q000201:enzymology; D000818:Animals; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D004285:Dogs; D005260:Female; D005727:Ganglia, Spinal / Q000201:enzymology*; D008297:Male; D009416:Nerve Regeneration; D009474:Neurons / Q000201:enzymology; D012584:Sciatic Nerve / Q000201:enzymology*
chemical_list: D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase