Isolation of bacteriophages T2 and T4 attached to the outer membrane of Escherichia coli.
Phage T2 or T4 was adsorbed to Escherichia coli, and the outer (L) membrane was then isolated with the phage still attached in their usual postinjection appearance. T2 was readily inactivated by isolated cell walls but very poorly by purified L membrane. T4 was inactivated by neither.
D000327:Adsorption; D002021:Buffers; D002473:Cell Wall; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D003090:Coliphages / Q000302:isolation & purification*; D004926:Escherichia coli; D008070:Lipopolysaccharides / Q000302:isolation & purification*; D008274:Magnesium; D008722:Methods; D008854:Microscopy, Electron; D010772:Phosphotungstic Acid; D013194:Staining and Labeling; D013395:Sucrose; D014325:Tromethamine
D002021:Buffers; D002712:Chlorides; D008070:Lipopolysaccharides; D014325:Tromethamine; D010772:Phosphotungstic Acid; D002586:Cesium; D013395:Sucrose; D008274:Magnesium