title: Elevated bile acids in the plasma of laying hens fed rapeseed meal.
abstract: A simple procedure was developed for the estimation of bile acid taurine conjugates in fowl plasma. Laying hens fed a diet containing rapeseed meal (RSM) (400 g kg-1) for 12 weeks had higher bile acid levels (154 mumol litre-1) than hens fed a control soyabean diet (116 mumol litre-1) (P less than 0.01). The incidence of liver haemorrhages was higher (34.8 per cent) in RSM-fed hens than in controls (21 per cent), but the severity of the lesions did not correlate with the bile acid concentration in affected birds. Histological examination of sections from livers of RSM-fed birds did not reveal significant hepatocyte degeneration outside the immediate vicinity of the haemorrhage. Canalicular bile plugs were never seen. The incidence of liver haemorrhages (13 per cent) and plasma bile acids (85 mumol litre-1) were lower in hens fed a diet containing beta-aminopropionitrile (0.5 g kg-1), a known lathyrogen. Acute treatment of hens with the hepatotoxin alpha-naphthyl isothiocyanate over four days induced necrosis of hepatocytes and resulted in elevated bile acid concentrations (262 mumol litre-1) compared with controls (73 mumol litre-1) given arachis oil. It was concluded that laying hens fed high levels of RSM develop cholestasis but the toxic principle is not known.
mesh_terms: D000821:Animal Feed / Q000009:adverse effects*; D000818:Animals; D001647:Bile Acids and Salts / Q000032:analysis / Q000097:blood*; D001937:Brassica; D002645:Chickens / Q000097:blood*; D002855:Chromatography, Thin Layer; D005260:Female; D006470:Hemorrhage / Q000097:blood / Q000209:etiology / Q000473:pathology / Q000662:veterinary; D008099:Liver / Q000473:pathology; D008107:Liver Diseases / Q000097:blood / Q000209:etiology / Q000473:pathology / Q000662:veterinary; D011201:Poultry Diseases / Q000097:blood / Q000209:etiology / Q000473:pathology
chemical_list: D001647:Bile Acids and Salts