title: Conversion from 4S androgen receptor from rat submandibular gland to higher molecular form and the effect of sodium molybdate.
abstract: The androgen receptor from rat submandibular gland was transformed by exposure to ATP at 0 degrees C. The transformed 4 S receptor converted to a higher molecular weight form in a low-salt glycerol gradient centrifugation when ATP was removed from the sample. The sedimentation coefficient of the converted receptor was similar in the absence or presence of 20 mM molybdate; 7.8 +/- 0.5 S without molybdate and 7.6 +/- 0.3 S with molybdate. However, the receptor converting in the presence of molybdate could markedly bind to DNA-cellulose, while an entity without molybdate could not. These results suggest that molybdate directly interacts with the DNA-binding domain on the 4 S androgen receptor and prevents this domain from being concealed by conversion in low-salt conditions.
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002482:Cellulose / Q000031:analogs & derivatives / Q000378:metabolism; D002850:Chromatography, Gel; D004247:DNA / Q000031:analogs & derivatives / Q000378:metabolism; D005260:Female; D066298:In Vitro Techniques; D008970:Molecular Weight; D008982:Molybdenum / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D011944:Receptors, Androgen / Q000187:drug effects / Q000378:metabolism*; D013363:Submandibular Gland / Q000378:metabolism*
chemical_list: C033190:DNA-cellulose; D011944:Receptors, Androgen; D008982:Molybdenum; D000255:Adenosine Triphosphate; D002482:Cellulose; D004247:DNA; C024687:sodium molybdate(VI)