Extraction, radioiodination, and in vivo catabolism of equine fibrinogen.
Equine fibrinogen was isolated and aliquots were stored frozen at -70 C before radiolabeling with 125I (half-life = 60.2 days; gamma = 35 keV, using monochloroiodine reagent. Radioiodination efficiencies were 49% to 53%, resulting in a labeled product with 98% protein-bound activity and 91% clottable radioactivity. In 6 equine in vivo investigations, plasma half-lives of 125I-labeled fibrinogen were from 4.1 to 5.2 days, corresponding to a mean daily plasma elimination rate of approximately 15%.
D000818:Animals; D005260:Female; D005340:Fibrinogen / Q000302:isolation & purification / Q000378:metabolism*; D006207:Half-Life; D006736:Horses / Q000378:metabolism*; D007457:Iodine Radioisotopes; D007700:Kinetics; D008297:Male
D007457:Iodine Radioisotopes; D005340:Fibrinogen