Scanning electron microscopical investigations on the respiratory epithelium of the Syrian golden hamster. VI. In vitro effects of different polycyclic aromatic hydrocarbons.
In a comparative study, the biological effects of different polycyclic aromatic hydrocarbons (PAH) on the fetal respiratory epithelium of Syrian golden hamsters were examined (light and scanning electron microscopy) in tracheal explants exposed in vitro to benz(a)anthracene (BaA), benz(a)acridine (BaAC), benzo(b)fluoranthene (BbF), benzo(e)pyrene (BeP), benzo(k)fluoranthene (BkF) and pyrene (PYR). Concentrations of the compounds related to the solubility in dimethylsulphoxide in the tissue culture medium (0.5% DMSO). Focally slight inhibition of epithelial differentiation and/or circumscribed simple metaplasia with an incidence of up to 10% were found in the controls (0.5% DMSO), BeP and PYR groups as well as in the explants exposed to low levels of BaA and BaAC. The frequency of these lesions increased with raising (doubling) the dose (50%: BaAC, BbF, BfK; 100%: BaA). Following exposure to the latter compounds a similar dose response relationship was observed for dysplastic alterations of the respiratory epithelium. In some of these cases (10%) the epithelial change was associated with focally hyperplastic and/or proliferative exophytic growth. Morphologically, the alterations were comparable to those representing early changes associated with the development of epidermoid and mucoepidermoid neoplasms found after in vivo PAH exposure. In terms of transformation, the data support the information obtained from observations on mixed fetal hamster lung cells exposed to the same compounds.
D000818:Animals; D002923:Cilia / Q000187:drug effects; D006224:Cricetinae; D004848:Epithelium / Q000187:drug effects; D008647:Mesocricetus; D008855:Microscopy, Electron, Scanning; D011083:Polycyclic Compounds / Q000494:pharmacology*; D014132:Trachea / Q000187:drug effects*
D011083:Polycyclic Compounds