Effects of adriamycin on enzymes of adenine nucleotide metabolism in heart cell cultures.
Effects of adriamycin (ADM) treatment on rat heart cells in culture were examined. ADM treatment (lug/ml) for 3 hr, 48 to 51hr of culture age, had no effect on oxygen consumption, myosin content or myosin ATPase activity. In contrast, the activities of creatine kinase, adenylate kinase and adenosine deaminase were significantly lower in ADM-treated cells than in corresponding controls at 72 and 96 hr of culture age. Fall in the activities of these key enzymes of adenine nucleotide metabolism would lead to disturbances in energy metabolism, and results in the functional impairment of heart cells.
D000227:Adenine Nucleotides / Q000378:metabolism*; D000243:Adenosine Deaminase / Q000378:metabolism; D000263:Adenylate Kinase / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D003402:Creatine Kinase / Q000097:blood; D004317:Doxorubicin / Q000494:pharmacology*; D009206:Myocardium / Q000201:enzymology*; D009218:Myosins / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects; D011506:Proteins / Q000378:metabolism; D051381:Rats
D000227:Adenine Nucleotides; D011506:Proteins; D004317:Doxorubicin; D003402:Creatine Kinase; D000263:Adenylate Kinase; D000243:Adenosine Deaminase; D009218:Myosins