The incorporation of O6-methyldeoxyguanosine monophosphate and O4-methyldeoxythymidine monophosphate into polynucleotide templates leads to errors during subsequent in vitro DNA synthesis.
O4-MedTMP and O6-MedGMP may be incorporated from their 5'-triphosphates into polynucleotides by DNA polymerases. Once incorporated O6-MedGMP leads to errors during further cycles of DNA synthesis with a mutagenic efficiency of 0.3-0.7, the value depending upon the relative dNTP concentrations in the assay. In contrast O4-MedTMP leads to very few errors following its incorporation into DNA. The results demonstrate that O6-MedGMP, once incorporated into DNA, should be destined to give rise to DNA base changes during the second round of DNA synthesis.
D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D003848:Deoxyguanine Nucleotides / Q000378:metabolism*; D008745:Methylation; D009154:Mutation; D011089:Polydeoxyribonucleotides / Q000378:metabolism*; D013379:Substrate Specificity; D013698:Templates, Genetic; D013936:Thymidine / Q000031:analogs & derivatives* / Q000378:metabolism; D014316:Tritium
D003848:Deoxyguanine Nucleotides; D011089:Polydeoxyribonucleotides; D014316:Tritium; C008505:O-methylthymidine; C018601:O(6)-methyldeoxyguanylic acid; D004259:DNA-Directed DNA Polymerase; D013936:Thymidine