Rapid detection of small numbers of airborne bacteria by a membrane filter fluorescent-antibody technique.
Bacteria from stirred settling aerosols were recovered and identified by a rapid fluorescent-antibody technique previously described by Danielsson. Calibrated air samples from Serratia marcescens aerosols were drawn through a Millipore aerosol filter holder fitted out with a nonfluorescent membrane filter (type HAB 047). With the aid of a high-power incident-light ultraviolet microscope, the individual bacterial cells, trapped on the filter, could be inspected after staining with appropriate conjugate on the filter surface.
D000336:Aerosols; D000391:Air Microbiology; D000818:Animals; D001409:Bacillus cereus / Q000302:isolation & purification; D001419:Bacteria / Q000302:isolation & purification*; D004926:Escherichia coli / Q000302:isolation & purification; D005374:Filtration; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D008567:Membranes, Artificial; D008722:Methods; D008856:Microscopy, Fluorescence; D011817:Rabbits; D012706:Serratia marcescens / Q000302:isolation & purification; D013194:Staining and Labeling
D000336:Aerosols; D005452:Fluoresceins; D007106:Immune Sera; D008567:Membranes, Artificial