title: Cellular heterogeneity in the expression of the delta-crystallin gene in non-lens tissue.
abstract: RNA transcripts of the delta-crystallin genes, which code for the major chicken lens protein, have been detected at low levels in many non-lens tissues. Here it is demonstrated by in situ hybridisation that these transcripts are concentrated at a high level in small, infrequent clusters of cells in many non-lens tissues. While the nuclei of these cells are very heavily labelled, there is only light labelling of the cytoplasm. The unlabelled cells surrounding the labelled clusters are of similar morphology and staining properties as the labelled cells, and all have the characteristic morphology of cells of the embryonic tissue used. With the exception of neural retina, it is not yet known whether the labelled clusters are found in specific locations in the tissues, or whether they arise at random.
mesh_terms: D000818:Animals; D002642:Chick Embryo; D003459:Crystallins / Q000235:genetics*; D003851:Deoxyribonucleases / Q000378:metabolism; D004838:Epiphyses / Q000032:analysis; D005786:Gene Expression Regulation; D009206:Myocardium / Q000032:analysis; D009693:Nucleic Acid Hybridization; D010857:Pigment Epithelium of Eye / Q000032:analysis; D010903:Pituitary Gland, Anterior / Q000032:analysis; D010957:Plasmids; D012160:Retina / Q000032:analysis; D012260:Ribonucleases / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D003459:Crystallins; D003851:Deoxyribonucleases; D012260:Ribonucleases