title: Interactive effects of organic and inorganic selenium with cadmium and mercury on spermatozoal oxygen consumption and motility in vitro.
abstract: The effects of cadmium (Cd), mercury (Hg), and three different chemical forms of selenium (Se) (selenite, selenocystine, and selenomethionine) on ram spermatozoal motility and oxygen consumption in vitro were studied over a 4-mo period. Concentrations of 10(-6) to 10(-2) M Cd and Hg were injurious to spermatozoa as indicated by depressed motility and reduced oxygen uptake. Equimolar concentrations of Se as selenite, selenocystine, or selenomethionine counteracted the toxicity of Cd and Hg at low concentrations (10(-5) and 10(-6) M) but not at higher concentrations (10(-4) to 10(-2) M). Gel filtration (Sephadex G-75) of seminal plasma and solubilized sperm prepared from semen incubated with Cd or Hg with or without the Se compounds revealed that Cd or Hg eluted with the void volume proteins in all treatments. Incubation of ram spermatozoa with any of the three chemical forms of Se ranging from 10(-6) to 2.5 X 10(-5) M significantly improved sperm motility and oxygen consumption.
mesh_terms: D000818:Animals; D002104:Cadmium / Q000494:pharmacology*; D003553:Cystine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004347:Drug Interactions; D007700:Kinetics; D008297:Male; D008628:Mercury / Q000494:pharmacology*; D016566:Organoselenium Compounds; D010101:Oxygen Consumption / Q000187:drug effects*; D020887:Selenious Acid; D012643:Selenium / Q000494:pharmacology*; D012645:Selenomethionine / Q000494:pharmacology*; D012756:Sheep; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism*; D013329:Structure-Activity Relationship
chemical_list: D016566:Organoselenium Compounds; D002104:Cadmium; C009226:selenocystine; D003553:Cystine; D012645:Selenomethionine; D020887:Selenious Acid; D008628:Mercury; D012643:Selenium