Variations in the localization of acetyl-coenzyme A synthetase in aerobic yeast cells.
In cells of Saccharomyces cerevisiae growing aerobically for 24 hr, acetyl-coenzyme A synthetase [acetate: CoA ligase (AMP), EC 6.2.1.1] was localized principally in the microsomal fraction. On density gradients, the enzyme in such cells behaved as a low-density particle, readily separable from the soluble proteins. After 48 hr of incubation, the cells showed a bimodal distribution of enzyme, with most of the activity now sedimenting with the mitochondrial fraction and only a smaller amount with the microsomal fraction. By using density gradients, two forms of synthetase were obtained from these cells: one band denser and the other band less dense than the intact mitochondria. In all preparations containing synthetase activity, appreciable levels of phospholipids were also detected.
D002021:Buffers; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D002712:Chlorides; D002794:Choline / Q000378:metabolism; D003470:Culture Media; D003576:Electron Transport Complex IV / Q000378:metabolism; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D008025:Ligases / Q000302:isolation & purification* / Q000378:metabolism; D008274:Magnesium; D008854:Microscopy, Electron; D008861:Microsomes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D010100:Oxygen; D012440:Saccharomyces / Q000201:enzymology* / Q000254:growth & development; D013012:Sorbitol; D013194:Staining and Labeling; D013385:Succinate Dehydrogenase / Q000378:metabolism; D014325:Tromethamine
D002021:Buffers; D002247:Carbon Isotopes; D002712:Chlorides; D003470:Culture Media; D014325:Tromethamine; D013012:Sorbitol; D007521:Isocitrate Dehydrogenase; D013385:Succinate Dehydrogenase; D003576:Electron Transport Complex IV; D008025:Ligases; D008274:Magnesium; D002794:Choline; D010100:Oxygen