title: Development and neuronal organization of dissociated and reaggregated embryonic cerebellum after intracephalic transplantation to adult rodent recipients.
abstract: Embryonic (E13) rat cerebellar primordia were dissociated and aggregated into tissue pellets by centrifugation. After transplantation into premade intracephalic cavities in adult rats, transplant development was evaluated at 2, 4 and 6 weeks survival. Within the cerebellar pellets there is an initial sorting of large neurons (Purkinje cells from deep nuclear cells) followed by the segregation of developing cortical cells into a trilaminar organization. These results suggest that this preparation should be useful for analyzing cellular interactions that determine cerebellar cytoarchitectural organization.
mesh_terms: D000818:Animals; D002449:Cell Aggregation; D002469:Cell Separation; D002531:Cerebellum / Q000166:cytology / Q000196:embryology* / Q000637:transplantation; D051381:Rats; D011919:Rats, Inbred Strains