[Ultrastructural study on the nucleus pulposus of the inter-vertebral disc--the behavior of the cells in the nucleus pulposus and their autolytic changes in the monkey].
Ultrastructural studies were carried out to examine the normal structure and postmortem autolytic changes of the cells and matrix of nucleus pulposus using adult monkey. Two kinds of cells were observed in the nucleus pulposus. The one was chondrocyte, which contained normal organelles and that was characterized by large halo. The halo was composed of numerous "crista like structures", that were regarded to form the matrix of nucleus pulposus. The other was notochordal cell, most of which appeared in grouping or separately, and yet had cell activity. In addition, there were the intermediate type of cells between chondrocyte and notochordal cell. The ultrastructural autolytic changes were rarely seen in the cells of 6 hours after death, but the changes in the halo and in the cytoplasm were remarkable after more than 12 hours. The autopsied nucleus pulposus for electron microscopical examination should be used within 6 hours after death in usual room temperature.
D000818:Animals; D001329:Autolysis; D005260:Female; D007403:Intervertebral Disc / Q000166:cytology / Q000648:ultrastructure*; D008252:Macaca fascicularis; D008297:Male; D008854:Microscopy, Electron; D013997:Time Factors