Isolation of high-density lipoproteins by immunoaffinity column chromatography from hog plasma.
High density lipoprotein (HDL) was isolated from hog plasma by a simple immunoaffinity column chromatography procedure using immobilized anti-apolipoprotein AI. The composition of HDL isolated by immunoaffinity chromatography was nearly identical to that of a control sample that was isolated by an alternate method utilizing ultracentrifugation and gel chromatography. The HDL isolated by immunoaffinity chromatography had a larger number of polypeptide components that the control as indicated by acrylamide gel electrophoresis in the presence of urea. When the HDL isolated by immunoaffinity chromatography was applied to a heparin-agarose column the amount of protein retained was approximately twice that of the control. These findings indicate that the ultracentrifugation procedure probably induced the loss of apolipoprotein E containing components from the HDL complex.
D000818:Animals; D000936:Antigen-Antibody Complex; D002784:Cholesterol / Q000032:analysis; D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D008075:Lipoproteins, HDL / Q000097:blood* / Q000302:isolation & purification; D010743:Phospholipids / Q000032:analysis; D013552:Swine; D014280:Triglycerides / Q000032:analysis
D000936:Antigen-Antibody Complex; D008075:Lipoproteins, HDL; D010743:Phospholipids; D014280:Triglycerides; D002784:Cholesterol