title: 1-Alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet activating factor) stimulates plant H+ transport in vitro and growth.
abstract: 1-Alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet activating factor = PAF), a lipid with hormone-like properties in mammals, strongly stimulated ATP-dependent H+ transport in microsomes from zucchini hypocotyls. Stimulation of the maximal delta pH was 50-80% under optimal conditions at about 8 microM and growth was stimulated to about 20% at 2-4 microM 1-alkyl-2 acetyl-sn-glycero-3-phosphocholine. The effect on in vitro H+ transport was strongly dependent on the presence of phosphate. Chloride and nitrate were ineffective. Low concentrations of calcium interfered with the effect of 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine on in vitro H+ transport.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000494:pharmacology; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008861:Microsomes / Q000378:metabolism; D010710:Phosphates / Q000494:pharmacology; D063245:Plant Development; D010944:Plants / Q000187:drug effects* / Q000378:metabolism; D010972:Platelet Activating Factor / Q000494:pharmacology*
chemical_list: D010710:Phosphates; D010972:Platelet Activating Factor; D006859:Hydrogen; D000255:Adenosine Triphosphate; D002118:Calcium