The binding of [3H]oestradiol-receptor complex to hypothalamic chromatin of male and female mice.
Histones and masking acidic proteins were removed from hypothalamic chromatin in order to evaluate/measure the number of available acceptor sites for the [3H]oestradiol-receptor complex. This number increases after dehistonizing and unmasking and is lower than published values for comparable preparations. No sex-related difference in [3H]oestradiol-receptor binding to hypothalamic chromatin in vitro was observed. Failure to observe such a difference suggests that sexual differentiation and steroid sensitivity cannot be attributed to marked differences in the degree of chromatin masking.
D000818:Animals; D002474:Cell-Free System; D005260:Female; D007031:Hypothalamus / Q000032:analysis*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011960:Receptors, Estrogen / Q000378:metabolism*; D012728:Sex Chromatin / Q000378:metabolism*; D012733:Sex Differentiation; D014316:Tritium
D011960:Receptors, Estrogen; D014316:Tritium