Phorbol ester activation of epidermal protein kinase C from tumor promotion sensitive and resistant mouse strains.
Calcium-stimulated, phospholipid dependent protein kinase C activity was found in cytosolic extracts of the epidermis of SENCAR, C57BL/6 and Ha/ICR mice. The enzymes were partially purified by DEAE-cellulose chromatography. There were no significant differences between the 3 strains in basal protein kinase C activity. The enzymes from all 3 strains were stimulated by 12-O-tetradecanoyl-phorbol-13-acetate (TPA) in the absence of calcium, but no correlation was seen between susceptibility to tumor promotion and any protein kinase C activity parameter measured.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002848:Chromatography, DEAE-Cellulose; D003600:Cytosol / Q000201:enzymology; D004351:Drug Resistance; D004789:Enzyme Activation; D004817:Epidermis / Q000201:enzymology; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D010704:Phorbols / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism*; D012867:Skin / Q000201:enzymology*; D013045:Species Specificity; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D010704:Phorbols; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium