[Effect of folic acid and methotrexate on the function of the hydroxylating system and the cholesterol and phospholipid content in the liver microsomes of rats].
Folic acid and its antivitamin methotrexate exert contrary actions on the activity of monooxygenases of the rat liver endoplasmatic membranes. Under the influence of folic acid (intra-abdominally, 100 mg/kg-6 days, 25 mg/k-14 days) there is a growth in the B5 and P-450 cytochrome content, NADP.H-cytochrome P-450 and NAD.H-cytochrome B5-reductase activity and in the oxidation rate of NADP.H, NAD.H and ethylmorphine N-demethylation. In similar experimental conditions, methotrexate (subcutaneously 0.25 and 1 mg/kg for 6 days, 0.25 mg/kg for 14 days) decreases the rate of NAD.H and NADP.H oxidation and NAD.H-cytochrome B5-reductase activity, the content of cytochromes B5 and P-450 and the binding degree of the latter with ethylmorphine, aniline and their metabolic rate. A decrease of the dose of the agents with a simultaneous increase of the time of administration enhances the vitamin action and reduces that of the antivitamin.
D000818:Animals; D002784:Cholesterol / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005492:Folic Acid / Q000494:pharmacology*; D006900:Hydroxylation; D008297:Male; D008727:Methotrexate / Q000494:pharmacology*; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D013997:Time Factors
D010743:Phospholipids; D005492:Folic Acid; D002784:Cholesterol; D006899:Mixed Function Oxygenases; D008727:Methotrexate
Vliianie folievoĭ kisloty i metotreksata na funktsiiu gidroksiliruiushcheĭ sistemy, soderzhanie kholesterina i fosfolipidov v mikrosomakh pecheni krys.