13C-NMR of ribosyl A-A-A, A-A-G, and A-U-G. Synthesis and assignment.
The three RNA trinucleotides; ApApA, ApApG, and ApUpG, have been synthesized in sufficient quantity to obtain natural abundance 13C(1H)-NMR spectra at strand concentrations between 4 and 100 mM. Comparisons between 70 degrees C spectra of the three trimers and their consistuent dimers ApA, ApG, ApU, and UpG allow secure assignments to be made for most of the resonances. This paper describes the syntheses and 13C assignments of the oligomers.
D000227:Adenine Nucleotides / Q000138:chemical synthesis; D001483:Base Sequence; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D013329:Structure-Activity Relationship
D000227:Adenine Nucleotides; D009843:Oligoribonucleotides; C048115:ApApG; C017020:ApUpG; C018832:adenylyl-(3'-5')-adenylyl-(3'-5')-adenosine