title: Analytical interference of drugs in clinical chemistry: II--The interference of three cephalosporins with the determination of serum creatinine concentration by the Jaffé reaction.
abstract: It is well known that some cephalosporins interfere with the Jaffé reaction for creatinine measurement. This interference varies according to the thirteen instruments and eighteen methodologies studied. Our study has shown that: the proportion of picric acid, NaOH and serum have only a slight effect on the interference; the addition of a buffer to the NaOH solution increases the interference due to cephalothin and cephaloridine, but lessens that due to cefoxitin; dialysis decreases the interference; in kinetic methods, the choice of times at which readings are made influences the magnitude of the interference; a higher incubation temperature increases the effect of cefoxitin and decreases the effects of cephalothin and cephaloridine; pre-incubation with NaOH decreases the interference due to cefoxitin, but has no significant effect on the interference due to cephalothin or to cephaloridine.
mesh_terms: D001322:Autoanalysis / Q000295:instrumentation*; D001774:Blood Chemical Analysis; D002021:Buffers; D002440:Cefoxitin / Q000494:pharmacology; D002509:Cephaloridine / Q000494:pharmacology; D002511:Cephalosporins / Q000494:pharmacology*; D002512:Cephalothin / Q000494:pharmacology; D003404:Creatinine / Q000097:blood*; D006801:Humans; D004364:Pharmaceutical Preparations / Q000097:blood*; D010851:Picrates / Q000494:pharmacology; D011930:Reaction Time; D012972:Sodium Hydroxide / Q000494:pharmacology; D013696:Temperature
chemical_list: D002021:Buffers; D002511:Cephalosporins; D004364:Pharmaceutical Preparations; D010851:Picrates; D012972:Sodium Hydroxide; D002440:Cefoxitin; C005858:picric acid; D003404:Creatinine; D002509:Cephaloridine; D002512:Cephalothin