Mitochondrial calcium and cellular electrolytes in brain cortex frozen in situ: electron probe analysis.
Mitochondrial calcium in rat brain cortex frozen in situ and measured with electron probe X-ray microanalysis was 1.5 mmol Ca/kg dry weight. Cellular, extramitochondrial Ca was 6.4mmol/kg dry weight and cytoplasmic Na+ was 73mM. These results do not support the role of mitochondria regulating cytoplasmic Ca2+, but are compatible with the regulation of mitochondrial enzymes by matrix free Ca2+.
D000818:Animals; D002118:Calcium / Q000032:analysis*; D002540:Cerebral Cortex / Q000032:analysis*; D004577:Electron Probe Microanalysis; D005615:Freezing; D008297:Male; D008928:Mitochondria / Q000032:analysis*; D011188:Potassium / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000032:analysis*
D012964:Sodium; D011188:Potassium; D002118:Calcium