Simultaneous separation and sensitive determination of free fatty acids in blood plasma by high-performance liquid chromatography.
The quantitative determination of saturated and unsaturated fatty acids (ranging from acetic acid to lignoceric acid) in biological samples is presented. The secondary amine group of 5-(dimethylamino)-1-naphthalenesulponyl-semipiperazide (dansyl-semipiperazide) reacts with the carboxyl group of the fatty acids to form an amide linkage in order to obtain fluorescent derivatives of the acids. The fluorescent derivatives are analysed by high-performance liquid chromatography (HPLC) using an internal standard.
D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D003619:Dansyl Compounds / Q000032:analysis / Q000138:chemical synthesis; D005230:Fatty Acids, Nonesterified / Q000097:blood* / Q000302:isolation & purification; D006801:Humans; D013050:Spectrometry, Fluorescence
D003619:Dansyl Compounds; D005230:Fatty Acids, Nonesterified