Liquid chromatographic determination of strychnine in stomach contents.
A chloroform extract of stomach contents at basic pH is concentrated and then extracted with 0.1 M phosphoric acid. The acid extract is chromatographed on a 10 cm reverse phase column, using 0.005 M phosphate buffer (pH 3.0)-acetonitrile-tetrahydrofuran (750 + 135 + 115) containing 0.01 M octanesulfonic acid at a flow rate of 1.0 mL/min for elution. Strychnine eluted in 7.3 min. Recoveries from spiked stomach contents averaged 92%. The method can be used without modification for other alkaloids.
D000470:Alkaloids / Q000032:analysis; D000818:Animals; D002853:Chromatography, Liquid; D004285:Dogs; D005766:Gastrointestinal Contents / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D013331:Strychnine / Q000032:analysis*
D000470:Alkaloids; D013331:Strychnine