Proton gradient-coupled uphill transport of glycylsarcosine in rabbit renal brush-border membrane vesicles.
An inward-directed proton gradient energizes the transport of intact glycylsarcosine against a concentration gradient in rabbit renal brush-border membrane vesicles. Dissipation of the proton gradient abolishes the uphill transport. Generation of an inside-negative membrane potential nearly doubles the intravesicular concentration of the dipeptide at the peak of the overshoot without altering the equilibrium value. These data provide direct evidence for peptide-proton cotransport in the renal brush-border membrane.
D000818:Animals; D001693:Biological Transport, Active; D004151:Dipeptides / Q000378:metabolism*; D006033:Glycylglycine / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D066298:In Vitro Techniques; D007476:Ionophores / Q000494:pharmacology; D007668:Kidney / Q000378:metabolism*; D008564:Membrane Potentials; D008871:Microvilli / Q000378:metabolism; D011817:Rabbits; D012964:Sodium / Q000378:metabolism
D004151:Dipeptides; D007476:Ionophores; D006033:Glycylglycine; C004194:glycylsarcosine; D012964:Sodium