Methylated pyrimidines stabilize an alternating conformation of poly(dA-dU).poly(dA-dU).
We have investigated the effect of increasing percentages of methylated pyrimidines on the structure of poly(dA-dU).poly(dA-dU). This was done by synthesizing analogous polynucleotides that contained deoxythymidine residues as well as deoxyuridine residues and observing their 31P NMR spectra in increasing amounts of CsF. The results show that methylated pyrimidines play a large role in the stabilization of the "alternating B" conformation of DNA.
D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008745:Methylation; D009690:Nucleic Acid Conformation; D011089:Polydeoxyribonucleotides / Q000138:chemical synthesis; D011743:Pyrimidines
D011089:Polydeoxyribonucleotides; D011743:Pyrimidines; C020468:poly(d(A-U))