An enzyme-linked immunosorbent assay (ELISA) for the detection of chlamydial antibodies in caprine sera.
Chlamydia psittaci is one of the major cause of abortion in goats. The complement-fixation (CF) test is widely used for detection of chlamydial antibodies in caprine sera. A simple, rapid enzyme-linked immunosorbent assay (ELISA) has been developed for the measurement of immunoglobulins G (IgG). The Chlamydia psittaci strain AB3 grown in yolk sac was used as antigen. ELISA proved satisfactory for the detection of serum IgG; a total of 204 sera were tested and the results correlated well with those obtained by CF test (80 per cent of agreement). The results obtained with ELISA were reproductible.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D002690:Chlamydia Infections / Q000175:diagnosis / Q000662:veterinary*; D002691:Chlamydophila psittaci / Q000276:immunology*; D003168:Complement Fixation Tests / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006041:Goats / Q000382:microbiology*; D014621:Vagina / Q000382:microbiology
D000907:Antibodies, Bacterial