A study of the selective inhibition by urea of neutrophil alkaline phosphatase isoenzymes.
The kinetics of neutrophil alkaline phosphatase (AP) inactivation by urea were examined. In standardized conditions (molarity and pH of buffer, magnesium chloride and urea concentration), monitoring of the enzymatic reaction allows identification of neutrophil AP isoenzymes. This study confirms the heterogeneity of AP in normal human neutrophils.
D000328:Adult; D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors* / Q000097:blood; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000037:antagonists & inhibitors* / Q000097:blood; D007700:Kinetics; D008297:Male; D009504:Neutrophils / Q000201:enzymology*; D014508:Urea / Q000494:pharmacology*
D007527:Isoenzymes; D014508:Urea; D000469:Alkaline Phosphatase