Comparative electrophoretic study between rabbit and human seminal fluid.
Cellulose acetate electrophoresis of rabbit seminal fluid give, after amidoschwartz staining, 19 protein bands, all migration towards the anode. Nine of them are present in at least 80% of the samples. With the acid of specific detection techniques, it was possible to demonstrate th presence of zinc in four bands, acid phosphatase activity in two and glycoprotein in three of the bands. Serum compounds seem to be present in small quantities. Study of fluids squeezed out from prostate, paraprostate, seminal vesicle and epididymal tail show few specific bands in these secretions: four in the case of prostate, three for paraprostate and one for seminal vesicle. Comparisons between human and rabbit seminal fluid electrophoresis show no relationship between these two types of secretion.
D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis; D004588:Electrophoresis, Cellulose Acetate; D004822:Epididymis / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D008297:Male; D011467:Prostate / Q000032:analysis; D011817:Rabbits; D012661:Semen / Q000032:analysis*; D012669:Seminal Vesicles / Q000032:analysis; D015032:Zinc / Q000032:analysis
D002352:Carrier Proteins; D006023:Glycoproteins; D000135:Acid Phosphatase; D015032:Zinc