title: The role of post-traumatic mitosis in elevation of anaerobic metabolism enzyme (lactic acid dehydrogenase) activity in degenerating central and peripheral nerve.
abstract: Glial and Schwann cells undergo marked biochemical and morphological alterations following axonal injury. In the present experiments, the extent of enzyme activity associated with anaerobic (LDH, lactic dehydrogenase) vs aerobic (SDH, succinic dehydrogenase) respiration was assessed distal to the site of nerve fiber injury. Studies were performed in rat central (optic) and peripheral (sciatic) nerves at 2, 7 and 14 days postoperatively (d.p.o.). In sciatic nerves, LDH activity rose 3-fold in traumatized (vs unoperated control) nerve tissue between 2 and 7 d.p.o. and remained elevated at 14 d.p.o. SDH activity in traumatized nerve was equal to that in unoperated nerve at 7 d.p.o., but decreased at 14 d.p.o. LDH activity in optic nerve at 2 d.p.o. was equivalent to that in control nerve, but rose approximately two-fold by 7 d.p.o. However, unlike peripheral nerve, activity in traumatized optic nerve decreased to control levels at 14 d.p.o. SDH activity in traumatized optic nerve remained unchanged at any timepoint examined. Taken in concert, these data are consistent with the hypothesis that there is an overall shift in CNS glial and Schwann cell metabolism from aerobic to anaerobic respiration following nerve injury. Additional studies were performed to determine if this shift requires prior Schwann or glial cell mitosis. Administration of mitotic inhibitor (AraC, cytosine arabinofuranoside) inhibited post-traumatic elevations in LDH activity in optic, but not peripheral nerve. No significant effect of the drug on axonal degeneration (as assessed by saxitoxin binding) was observed.
mesh_terms: D000818:Animals; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008938:Mitosis; D009410:Nerve Degeneration; D020221:Optic Nerve Injuries; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D012530:Saxitoxin / Q000378:metabolism; D013045:Species Specificity; D013127:Spinal Nerves / Q000293:injuries*; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
chemical_list: D012530:Saxitoxin; D007770:L-Lactate Dehydrogenase; D013385:Succinate Dehydrogenase; D013936:Thymidine