Growth of aortic smooth muscle cells from hypertensive obese and lean rats in homologous and heterologous sera.
Arterial smooth muscle cells (SMC) were derived from the medial thoracic aortas of three groups of rats: (a) obese, hyperlipidemic, hypertensive koletsky rats (OHT); (b) lean, normolipidemic, hypertensive koletsky rats (LHT) and (c) lean, normotensive Wistar rats (NT). The growth patterns of these cells were subsequently compared in tissue culture media (DMEM) supplemented with pooled 10% homologous or 10% heterologous sera. Morphologically the SMC from all three rat sources grew in culture in typical SMC hill and valley patterns. When the SMC from the three rat sources were cultured in DMEM supplemented with 10% newborn calf serum (NCS) there were no differences in final cell densities and morphologies between the cell types. However, SMC derived from OHT rat aortas grown in DMEM supplemented with pooled 10% homologous serum achieved lower cell densities than SMC derived from LHT and NT rat aortas in their respective homologous sera. There were no differences in final cell densities when LHT SMC and NT SMC were separately cultured inpooled heterologous sera derived from LHT, OHT or NT rats or when OHT cells were cultured in heterologous sera derived from either LHT or NT rats. The OHT serum preferentially affected only the growth and morphology of SMC derived from the obese, hyperlipidemic, hypertensive koletsky rats.
D000818:Animals; D002453:Cell Cycle; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D006973:Hypertension / Q000097:blood / Q000473:pathology*; D009131:Muscle, Smooth, Vascular / Q000473:pathology*; D009765:Obesity / Q000097:blood / Q000473:pathology; D051381:Rats
D003470:Culture Media