Cultivation of MDCK epithelial cells on chitosan membranes.
Deacetylated chitin upon evaporation from aqueous acetic acid solutions forms a thin, permeable and transparent porous membrane which can be successfully used as support of cell culture. An established MDCK cell line grown as monolayer on both chitosan membrane and millipore filter generates comparable bioelectrical properties when studied in a typical transporting chamber.
D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D002686:Chitin / Q000031:analogs & derivatives*; D048271:Chitosan; D004285:Dogs; D004847:Epithelial Cells; D006490:Hemostatics / Q000378:metabolism*; D007668:Kidney / Q000166:cytology; D008567:Membranes, Artificial
D006490:Hemostatics; D008567:Membranes, Artificial; D002686:Chitin; D048271:Chitosan