Affinity chromatography on immobilized hog gastric mucin and ovomucoid. A general method for isolation of lectins.
Two affinity adsorbents of general applicability for isolating of plant lectins are presented. The isolation of 30 lectins from 27 plants is described. The method works quickly and is inexpensive. In particular, mixtures of different lectins occurring in some plants may be resolved in one run.
D000818:Animals; D001789:Blood Group Antigens; D002846:Chromatography, Affinity; D004527:Egg Proteins / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D037102:Lectins / Q000302:isolation & purification*; D008970:Molecular Weight; D009077:Mucins / Q000032:analysis*; D010059:Ovomucin / Q000032:analysis*; D013552:Swine
D001789:Blood Group Antigens; D004527:Egg Proteins; D037102:Lectins; D009077:Mucins; D010059:Ovomucin