High-performance liquid chromatographic method for the determination of dolichols in tissues and plasma.
High-performance liquid chromatographic methods for the determination of dolichols in tissues and plasma have been developed. The tissue concentration of dolichols was measured by high-performance liquid chromatography with uv detection and plasma levels of dolichols were determined fluorometrically after derivatization with anthracene-9-carboxylic acid. In both methods, 2,2-didecaprenylethanol was used as an internal standard. The method with fluorescence detection was sufficiently sensitive to measure the concentration of dolichols in human plasma.
D000328:Adult; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000032:analysis*; D004286:Dolichols / Q000031:analogs & derivatives / Q000032:analysis* / Q000097:blood; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D004224:Diterpenes; D004286:Dolichols