Defective virions of reovirus.
When purified preparations of stock reovirus, type 3, were digested with chymotrypsin, the virions were converted into two different types of particle. These new particles could be separated from each other by isopycnic centrifugation in cesium chloride gradients. One particle banded at a buoyant density of 1.43 g/cm(3), the other at a density of 1.415 g/cm(3). The former particle is termed the heavy (H) particle, the latter is the light (L) particle. The ratio of H/L particles varied between 0.5 and 0.25 in various purified preparations of virus. In electron micrographs, both H and L particles had the appearance and dimensions of viral cores. H particles were infectious for L cells. When plaques formed by stock virus, or by H particles, were picked and propagated in L cells, the majority of the clones gave rise only to H particles on chymotrypsin digestion. On continued serial passage of the clones, virions containing L particles again appeared in the progeny. The simplest explanation of these results was that stock virus was comprised of two populations of virions. One type of virion which contained H particles was infectious, whereas the other, which contained L particles, was not itself infectious and could replicate only in cells coinfected with an H particle virion. Added weight was given to this hypothesis by two observations. First, a small but definite separation of H and L virions could be achieved by isopycnic centrifugation in a gradient of cesium chloride. Second, L particles and virions containing L particles were both shown to lack the largest of the ten segments of double-stranded ribonucleic acid genome. Thus, L particle virions have defective genomes.
D000179:Acrylates; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D002918:Chymotrypsin / Q000494:pharmacology; D003588:Cytopathogenic Effect, Viral; D004586:Electrophoresis; D005782:Gels; D005827:Genetics, Microbial; D006367:HeLa Cells; D008854:Microscopy, Electron; D010772:Phosphotungstic Acid; D012316:RNA Nucleotidyltransferases / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D012087:Reoviridae / Q000032:analysis / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000472:pathogenicity; D013194:Staining and Labeling; D014316:Tritium; D014500:Uracil Nucleotides / Q000378:metabolism; D014529:Uridine / Q000378:metabolism; D014779:Virus Replication
D000179:Acrylates; D002247:Carbon Isotopes; D002712:Chlorides; D005782:Gels; D012367:RNA, Viral; D014500:Uracil Nucleotides; D014316:Tritium; D010772:Phosphotungstic Acid; D002586:Cesium; D012316:RNA Nucleotidyltransferases; D002918:Chymotrypsin; D014529:Uridine