[The influence and significance of obtaining, storing and assay on estrogen receptor (ER) content in breast cancer tissue].
Estrogen receptor (ER) was measured in 275 samples from 170 patients with breast cancer by DCC assay. The results showed that the ER status and content of cytosol separated by ultracentrifuge (105,000 X G) or orthocentrifuge (1,200 X G) confirmed well (P greater than 0.5). Therefore, the ordinary centrifuge is suitable. But there was a poor correlation between the ER level measured by DCC method and that by Lee's cytochemical method (P greater than 0.05). The store time of the sample in liquid nitrogen did not seem to effect much on the ER positive rate (chi 2 = 0.7686). The ER status in samples obtained from the primary or metastatic lesion or at different intervals were generally similar in the same patient (P greater than 0.2). It is suggested that the ER status be an inherent property of patients with breast cancer. The authors propose that more samples be taken in order to render the determination more reliable.
D001943:Breast Neoplasms / Q000032:analysis* / Q000276:immunology; D006801:Humans; D008722:Methods; D011960:Receptors, Estrogen / Q000032:analysis*
D011960:Receptors, Estrogen