Labelling of granulocytes with radioindium: cell isolation and labelling parameters.
A method for isolation and labelling of purified granulocytes with 111In-oxine for scintigraphy of inflammatory processes is described. The procedure, on average, yielded 27.6% of the initial granulocytes in the sample with minimal lymphocyte and platelet contamination. The mean erythrocyte/granulocyte ratio was 0.95. In 20 experiments, 85.3% of the total radioactivity was attached to granulocytes, 8.7% to erythrocytes and 5.9% was in the plasma. The sensitivity and the specificity for the detection of acute inflammatory processes in 44 patients were 91.3% and 81.8%, respectively.
D001419:Bacteria / Q000302:isolation & purification; D002469:Cell Separation / Q000379:methods*; D004760:Enterocolitis / Q000000981:diagnostic imaging; D004906:Erythrocyte Count; D006098:Granulocytes / Q000032:analysis*; D006801:Humans; D007204:Indium; D007239:Infections / Q000000981:diagnostic imaging; D007249:Inflammation / Q000000981:diagnostic imaging*; D007553:Isotope Labeling / Q000379:methods*; D007958:Leukocyte Count; D011868:Radioisotopes; D011877:Radionuclide Imaging; D013048:Specimen Handling
D011868:Radioisotopes; D007204:Indium