[Study on detection of metastatic lymph nodes from gastric cancer--use of hematoporphyrin-injection in submucosal layer of the stomach].
It is well known that hematoporphyrin (Hp) has the affinity for malignant tissue and the deposition of Hp can be visualized by ultraviolet (UV) irradiation as red fluorescence. This study was attempted to detect the metastatic lymph nodes from gastric cancer at the time of operation, utilizing Hp and UV irradiation. Forty eight hours before the operation, 2ml of Hp solution was endoscopically injected into the submucosal layer neighbouring the cancerous lesion of the stomach. Deposition of Hp in lymph nodes was detected as red fluorescence by UV irradiation during the operation. Resected lymph nodes were reexamined by the same procedure and the histological examination was also performed. The detection rate for metastatic lymph node by UV irradiation during the operation was 78.9%, which was confirmed by histological examination retrospectively. However, this procedure was accompanied by 8.6% of false negative. Postoperative evaluation of resected lymph nodes revealed that histological metastasis rate from lymph nodes which had the red fluorescence was 56.9% and that from lymph nodes which had no fluorescence was 13.2%. Present study suggested that endoscopic injection of Hp was of use in detecting the metastatic lymph nodes from gastric cancer at the time of operation.
D000818:Animals; D005453:Fluorescence; D005753:Gastric Mucosa; D006415:Hematoporphyrins / Q000378:metabolism; D006801:Humans; D007267:Injections; D008197:Lymph Node Excision; D008198:Lymph Nodes / Q000378:metabolism; D008207:Lymphatic Metastasis; D051379:Mice; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D014466:Ultraviolet Rays
D006415:Hematoporphyrins