[Plasma assay of haloperidol by high performance liquid chromatography].
Haloperidol is extracted with hexane-isoamyl alcohol in the presence of two internal standards, chlorohaloperidol and spiroperidol. For chromatographic separation, a C18 reversed-phase column and a mobile phase of acetonitrile/methanol/phosphate buffer are used. Detection is by UV absorption spectrophotometry at 245 nm, and the limit of detection was set to 1 ng/ml. To study hydroxyhaloperidol, a metabolite of haloperidol which has much less UV-absorption than haloperidol, detection must be seat 220 nm.
D002851:Chromatography, High Pressure Liquid; D006220:Haloperidol / Q000097:blood*; D006801:Humans
D006220:Haloperidol
Dosage plasmatique de l'halopéridol par chromatographie liquide haute performance.