Studies on steroids. CCXVI. Separation of bile acid 3-glucuronides by high-performance liquid chromatography.
The separation of 3-glucuronides of cholate, chenodeoxycholate, deoxycholate, ursodeoxycholate and lithocholate, and their glyco- and tauro-conjugates, has been carried out by high-performance liquid chromatography on a reversed-phase column. The chromatographic behaviour of bile acid 3-glucuronides was dependent on the type of conjugation. An effect of the pH of the mobile phase on the capacity ratio was observed at higher pH for chenodeoxycholate 3-glucuronide, probably owing to steric interaction of the 7 alpha-hydroxy group with the carboxy group in the glucuronyl moiety. Conversion of the alpha-hydroxy function on the steroid nucleus into an oxo group resulted in a 50% decrease in the capacity ratio. Bile acid 3-glucuronides were efficiently separated on Shodex ODS Pak F-411 using three kinds of ammonium phosphate buffer-acetonitrile systems.
D001647:Bile Acids and Salts / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D005965:Glucuronates / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D013256:Steroids / Q000302:isolation & purification*
D001647:Bile Acids and Salts; D005965:Glucuronates; D013256:Steroids