title: On the isolation of the thrombin inhibitor hirudin.
abstract: A procedure for isolation of hirudin from crude preparations was described. By the use of ion exchange chromatography and affinity chromatography preparations were obtained with a specific activity of 10 to 15 antithrombin units/micrograms. Separation into several fractions with the same activity suggests the existence of isoinhibitors.
mesh_terms: D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006629:Hirudins / Q000302:isolation & purification* / Q000494:pharmacology; D007700:Kinetics; D007865:Leeches; D013917:Thrombin / Q000378:metabolism
chemical_list: D006629:Hirudins; D013917:Thrombin