[3H-thymidine incorporation into the DNA of the nucleic factor in the ascitic fluid of Ehrlich cancer].
After intraperitoneal administration of 1.85 MBq 3H-thymidine to mice with Ehrlich ascites tumours the maximal radioactivity of nucleic factor DNA of ascitic fluid was observed when the isotope had been injected 5-6 days before extraction of ascitic fluid (4-5.10(5) cpm/mg). The maximal radioactivity of DNA of tumour cells themselves was registered when 3H-thymidine was administered 2 days prior to tumour ascites extraction (5.2.10(5) cpm/mg). The administration of even 37 MBq 3H-thymidine 1.5 h before the ascitic fluid extraction from the Ehrlich ascites tumours did not result in the incorporation of this radioactive precursor into nucleic factor DNA. The results obtained show that there is an interval (several days) between the nucleic factor DNA synthesis and its secretion.
D000818:Animals; D001202:Ascitic Fluid / Q000378:metabolism*; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000032:analysis / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D013936:Thymidine / Q000378:metabolism*; D013997:Time Factors; D014316:Tritium
D004273:DNA, Neoplasm; D014316:Tritium; D013936:Thymidine
Vkliuchenie 3H-timidina v DNK nukleinovogoo faktora astsitnoĭ zhidkosti raka Erlikha.