Studies on the distribution of the antigens detected by some newly prepared monoclonal antibodies in normal hemopoietic and leukemic cells.
A great number of hybridoma clones has been developed in two independent fusions after immunization with cells of a human non-T, non-B REH leukemic cell line, recognizing different differentiation antigens on leukocytes. Only four of these new murine monoclonal hybridoma-derived antibodies (Bra20, Bra30, BraFB6 and BraEA10) have been used in our study for determination of their reactivity spectrum in normal blood cells and fresh lymphoid and non-lymphoid leukemic cells. The resulting reactivity spectra of these four monoclonal antibodies in normal hemopoietic and leukemic cells in indirect immunofluorescence assay are in agreement with supposed Ia-like reactivity for Bra20, Bra30 and Bra FB6 monoclonal antibodies and "common" leukocyte specificity for BraEA10 monoclonal antibody.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D001792:Blood Platelets / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D006098:Granulocytes / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000276:immunology; D006801:Humans; D007938:Leukemia / Q000276:immunology*; D007962:Leukocytes / Q000276:immunology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D008223:Lymphoma / Q000276:immunology*; D009000:Monocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface