Structural investigation of the capsular polysaccharide of Klebsiella serotype K 49.
The structure of the capsular polysaccharide from Klebsiella type K 49 was investigated by 1H- and 13C-n.m.r. spectroscopy of the original, carboxyl-reduced, and Smith-degraded polysaccharides. Methylation of the original K 49 and derivatives showed that the polysaccharide consists of a tetrasaccharide repeating-unit having D-galacturonic as a single lateral substituent. All of the sugars have the alpha-D-configuration. This conclusion is in agreement with measurements of spin-lattice relaxation-times for the anomeric proton. O-Acetyl groups are located on galacturonic acid, but do not occupy a unique position. (Formula: see text).
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D007709:Klebsiella / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification*
D002241:Carbohydrates; D011135:Polysaccharides, Bacterial