Regulation of transepithelial ion transport by intracellular calcium ions.
A photodynamic effect of erythrosine B on the basolateral surface of rat colon epithelium under short circuit conditions is described. The resulting irreversible increase in short circuit current was the result of electrogenic chloride secretion. The effect was dependent upon oxygen and calcium ions, and is probably due to the generation of singlet oxygen which then permeabilises the membranes to calcium. Half maximal activation of secretion in permeabilised preparations occurred at an external calcium concentration of 1 microM. In tight sodium transporting epithelia increased Cai reduces SCC, possibly by a direct effect on apical sodium permeability. In toad urinary bladder SCC fell in response to conditions outlined above for rat colon.
D000584:Amiloride / Q000494:pharmacology; D000663:Amphibians; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D003106:Colon / Q000378:metabolism*; D004232:Diuretics / Q000494:pharmacology; D004848:Epithelium / Q000378:metabolism*; D006706:Homeostasis; D007700:Kinetics; D051381:Rats; D012867:Skin / Q000378:metabolism; D001743:Urinary Bladder / Q000378:metabolism*
D004232:Diuretics; D000584:Amiloride; D002118:Calcium