The effect of C-reactive protein on the PHA-induced proliferation of human peripheral blood lymphocytes.
The effect of CRP purified from ascites fluid originated from cancer patients on human peripheral blood lymphocytes was investigated. CRP was purified using 4-step procedure including absorption on Sepharose 4B, phosphocholine-Sepharose, DE-52 ion exchange chromatography and gel filtration on Sephacryl S-200. CRP was able either to inhibit or to enhance PHA-induced lymphocyte proliferation in vitro. The effects of added CRP depended on the time of its addition, dose of CRP and lymphocyte donor.
D002097:C-Reactive Protein / Q000276:immunology* / Q000302:isolation & purification; D004261:DNA Replication; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D009369:Neoplasms / Q000378:metabolism; D010835:Phytohemagglutinins
D010835:Phytohemagglutinins; D002097:C-Reactive Protein