[3H]N-Methylscopolamine binding to muscarinic receptors in intact adult rat brain cell aggregates.
Intact brain cell aggregates were dissociated from adult rat brains, by a simple sieving technique, and were used to study the binding characteristics of [3H]N-methylscopolamine to muscarinic acetylcholine receptors. The magnitude of binding of this ligand was related linearly to the amount of cell protein in the binding assay, with a high ratio of total to nonspecific binding. In addition, specific binding showed saturability and high affinity. Muscarinic receptor antagonists displaced specific [3H]N-methylscopolamine binding according to the law of mass-action, while it was possible to resolve displacement curves using receptor agonists into high- and low-affinity components. The results are discussed in terms of the usefulness of dissociated intact rat brain cells in studying muscarinic acetylcholine receptors in the central nervous system.
D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002449:Cell Aggregation; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D019832:N-Methylscopolamine; D051381:Rats; D011919:Rats, Inbred Strains; D011976:Receptors, Muscarinic / Q000378:metabolism*; D012602:Scopolamine Derivatives / Q000378:metabolism*; D014316:Tritium
D011976:Receptors, Muscarinic; D012602:Scopolamine Derivatives; D014316:Tritium; D019832:N-Methylscopolamine