Crystallization and preliminary X-ray analysis of the vitamin D-binding protein from human serum.
The vitamin D-binding protein from human serum has been crystallized from polyethylene glycol as a complex with 25-hydroxyvitamin D3 and examined by X-ray diffraction photography. The space group of the crystals is C2 with a = 203.0 A, b = 75.8 A, c = 90.9 A and beta = 109.5 degrees. There are two molecules of 56,000 dalton in the asymmetric unit. The crystals diffract to about 3.0 A resolution but the patterns exhibit a substantial level of diffuse scatter.
D003460:Crystallization; D006801:Humans; D011092:Polyethylene Glycols; D014809:Vitamin D-Binding Protein / Q000097:blood* / Q000302:isolation & purification; D014961:X-Ray Diffraction
D014809:Vitamin D-Binding Protein; D011092:Polyethylene Glycols