Enzymes of gluconate metabolism and glycolysis in Penicillium notatum.
In addition to the ability of Penicillium notatum to grow on sucrose, glucose, fructose and gluconate, substantial growth occurred on 2-ketogluconate and 5-ketogluconate thereby indicating a diverse sugar metabolism. Cell-free extracts contained all the enzymes of the Embden-Meyerhof-Parnas pathway and for both oxidative and non-oxidative pentose phosphate metabolism. Despite inconsistencies in results between different assay methods for the conventional Entner-Doudoroff (ED) enzymes, the data indicated the route was enzymatically possible. Demonstrations of the activities of the enzymes of the non-phosphorylative equivalent of the ED pathway were achieved. No evidence was found of a phosphorylative linking enzyme between the two pathways. Both 2- and 5-ketogluconate reductases were detected along with gluconate dehydrogenase which suggested interconvertibility between the ketogluconates and gluconate. However, ketogluconokinase, responsible for the conversion of ketogluconate to 2-keto-6-phosphogluconate, was not detected. A scheme for the interrelationships of routes of gluconate metabolism is discussed.
D050260:Carbohydrate Metabolism; D003470:Culture Media; D004798:Enzymes / Q000378:metabolism; D005942:Gluconates / Q000378:metabolism*; D006019:Glycolysis; D007700:Kinetics; D010407:Penicillium / Q000201:enzymology* / Q000254:growth & development
D003470:Culture Media; D004798:Enzymes; D005942:Gluconates