title: Dissociation and reassembly of Escherichia coli outer membrane and of lipopolysaccharide, and their reassembly onto flagellar basal bodies.
abstract: Purified lipopolysaccharide vesicles dissociate when treated with ethylenediaminetetraacetic acid (EDTA) and then reassemble when dialyzed against Mg(2+). Purified outer, lipopolysaccharide membrane (L membrane) is partially dissociated by treatment with EDTA and fully dissociated upon further treatment with Triton X-100. Both the partially and fully dissociated L membrane can be reassembled by dialysis against Mg(2+). Reassembly of lipopolysaccharide or L membrane in the presence of intact flagella results in specific attachment of flagellar basal bodies to vesicles via the L and sometimes the M ring. Lipopolysaccharide and L membrane appear to be composed of substructures bound together by both Mg(2+) (divalent cation)-mediated and hydrophobic bonds.
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis; D001669:Biochemical Phenomena; D001671:Biochemistry; D002462:Cell Membrane / Q000032:analysis; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D003956:Dialysis; D004492:Edetic Acid; D004926:Escherichia coli / Q000032:analysis / Q000166:cytology; D005407:Flagella; D008070:Lipopolysaccharides / Q000032:analysis / Q000302:isolation & purification; D008274:Magnesium; D008854:Microscopy, Electron; D010636:Phenols; D010772:Phosphotungstic Acid; D011523:Protoplasts; D012997:Solvents; D013194:Staining and Labeling; D013501:Surface-Active Agents
chemical_list: D001426:Bacterial Proteins; D002712:Chlorides; D008070:Lipopolysaccharides; D010636:Phenols; D012997:Solvents; D013501:Surface-Active Agents; D010772:Phosphotungstic Acid; D002586:Cesium; D004492:Edetic Acid; D008274:Magnesium