title: Structural studies on dissociated porcine gastric mucus gel.
abstract: Reduction of the insoluble porcine mucus gel with dithiothreitol, followed by molecular sieve chromatography, resulted in the recovery of the two fragments of molecular size 2.54 X 10(6) and 1.75 X 10(6). Both gastric mucus glycoproteins have been cleaved by pronase, papain or trypsin into fragments whose molecular sizes range from 5.60 X 10(5) to 1.87 X 10(6) daltons. On reduction, the enzyme-treated fragments were further dissociated into smaller fragments which have been characterized by SDS-PAGE. The evidence indicates that the pol: neric structure of gastric mucus differs from that of mucus of varying origins, in particular with respect to the size and number of component subunits per native molecule.
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005753:Gastric Mucosa / Q000032:analysis*; D008970:Molecular Weight; D009077:Mucins / Q000032:analysis*; D010206:Papain; D010446:Peptide Fragments / Q000032:analysis; D011402:Pronase; D013552:Swine; D014357:Trypsin
chemical_list: D009077:Mucins; D010446:Peptide Fragments; D014357:Trypsin; D010206:Papain; D011402:Pronase