Acute cardiopulmonary manifestations of smooth and rough strain lipopolysaccharide infusion in the conscious sheep.
We have characterized the acute cardiopulmonary response following smooth (055:B5) or rough strain (J5 and Re595) lipopolysaccharide (LPS) infusion (1 microgram/kg over 10 minutes) in the conscious sheep. All LPS strains induced in a pronounced increase in mean pulmonary artery pressure within 10 minutes of infusion and arterial PO2 fell between 20 and 30 torr. Cardiac output and stroke volume declined following LPS challenge while systemic vascular resistance increased. Heart rate exhibited a biphasic pattern, with an initial increase followed by a return to baseline and a subsequent elevation. Calculated oxygen consumption increased significantly following LPS infusion. Coincident with these cardiopulmonary events, the arterial neutrophil count declined precipitously, greater than 98% over 30 minutes, with a more rapid decline with the rough strains. Results indicate that the acute cardiopulmonary effects noted are components of a systemic inflammatory reaction elicited by LPS and the lipid A portion of LPS may be responsible for initiating the response.
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D001831:Body Temperature / Q000187:drug effects; D002199:Capillary Permeability / Q000187:drug effects; D005260:Female; D006321:Heart / Q000187:drug effects*; D006400:Hematocrit; D006863:Hydrogen-Ion Concentration; D008070:Lipopolysaccharides / Q000633:toxicity*; D008168:Lung / Q000187:drug effects*; D009504:Neutrophils / Q000187:drug effects; D010100:Oxygen / Q000097:blood; D010101:Oxygen Consumption / Q000187:drug effects; D012119:Respiration / Q000187:drug effects; D012756:Sheep; D013928:Thromboxane A2 / Q000096:biosynthesis
D008070:Lipopolysaccharides; D013928:Thromboxane A2; D010100:Oxygen