[Role of the transmembrane potential in impairing the barrier properties of erythrocyte membranes during cryopreservation].
Exposure of erythrocytes to nonpermeable cryoprotector solution (PEO-1500, sucrose) with reduced NaCl content resulted in the development of chlorine diffusion potential, calculated by the degree of medium acidulation. This was accompanied by the loss of intracellular K+ and the increase in hemolysis upon freezing and thawing.
D001793:Blood Preservation / Q000379:methods*; D002463:Cell Membrane Permeability / Q000187:drug effects; D003451:Cryoprotective Agents / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000502:physiology*; D005615:Freezing; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008564:Membrane Potentials / Q000187:drug effects; D011188:Potassium / Q000097:blood; D012965:Sodium Chloride / Q000494:pharmacology
D003451:Cryoprotective Agents; D012965:Sodium Chloride; D011188:Potassium
Rol' transmembrannogo potentsiala v narushenii bar'ernykh svoĭstv membran éritrotsitov v protsesse kriokonservatsii.