Demonstration of labile platelet desaggregation-promoting substance(s) in human blood, using the donor's own blood platelets ("DOP assay") as indicator cells, with special reference to the possibility of measuring diet-induced alterations.
A method is described for the semi-quantitative biological determination of labile platelet desaggregation-promoting substance(s) in human platelet suspensions and in platelet-free fresh plasma by using the donor's own platelets ("DOP assay"). Using the donor as his own control it is possible to demonstrate a dose-related increase of the platelet desaggregation-promoting potency in response to cod-liver oil. The labile platelet desaggregation-promoting activity can also be observed in fresh platelet-free plasma. Circumstantial evidence is presented for the prostaglandin-like nature of these labile platelet desaggregation-promoting substance(s).
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D001792:Blood Platelets / Q000502:physiology; D004032:Diet; D004837:Epinephrine / Q000494:pharmacology; D006403:Hematologic Tests / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D010974:Platelet Aggregation / Q000187:drug effects
D000244:Adenosine Diphosphate; D004837:Epinephrine