title: Fluorimetric determination of the quaternary compound trospium and its metabolite in biological material after derivatization with benoxaprofen chloride.
abstract: The quantitative determination of the quaternary compound trospium and the corresponding spiroalcohol is described for human biological material. Analysis is performed by alkaline hydrolysis, ion-pair extraction into chloroform, subsequent derivatization with the flurophor benoxaprofen chloride and high-performance liquid chromatographic separation on a reversed-phase column. The limit of quantitation is ca. 1 pmol (1.03 pmol=lng of trospium chloride) per millilitre of plasma.
mesh_terms: D001561:Benzilates; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006868:Hydrolysis; D009662:Nortropanes / Q000032:analysis* / Q000097:blood / Q000652:urine; D011422:Propionates
chemical_list: D001561:Benzilates; D009662:Nortropanes; D011422:Propionates; C011677:benoxaprofen; C003330:trospium chloride; C047182:3-hydroxynortropane-8-spiro-1'-pyrrolidinium