Development and neuronal organization of dissociated and reaggregated embryonic cerebellum after intracephalic transplantation to adult rodent recipients.
Embryonic (E13) rat cerebellar primordia were dissociated and aggregated into tissue pellets by centrifugation. After transplantation into premade intracephalic cavities in adult rats, transplant development was evaluated at 2, 4 and 6 weeks survival. Within the cerebellar pellets there is an initial sorting of large neurons (Purkinje cells from deep nuclear cells) followed by the segregation of developing cortical cells into a trilaminar organization. These results suggest that this preparation should be useful for analyzing cellular interactions that determine cerebellar cytoarchitectural organization.
D000818:Animals; D002449:Cell Aggregation; D002469:Cell Separation; D002531:Cerebellum / Q000166:cytology / Q000196:embryology* / Q000637:transplantation; D051381:Rats; D011919:Rats, Inbred Strains