Reversed-phase high-performance liquid chromatographic method for the assay of oxytetracycline.
The British Pharmacopoeia monograph for oxytetracycline calcium describes an high-performance liquid chromatographic (HPLC) assay which requires packing of the column by the analyst. Presented in this report is an HPLC method for the assay of oxytetracycline which employs a commercially available reversed-phase column and a solvent system which gives improved separation of the antibiotic from common impurities. Results obtained using this method for both bulk and dosage forms of oxytetracycline are in accord with the results of the microbiological assays.
D001407:Bacillus / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D010118:Oxytetracycline / Q000032:analysis* / Q000494:pharmacology; D013607:Tablets
D013607:Tablets; D010118:Oxytetracycline