[Reorganization of the protein composition of the nuclear matrix of hepatoma cells after inhibition of DNA synthesis with novobiocin].
The nuclear matrix of Zajdela hepatoma cells, in which DNA synthesis was blocked by novobiocin, contained 2.5-3.0 times more DNA and protein not dissociating in 2 M NaCl than the nuclear matrix of control cells. Chromatography of nuclear matrix preparations on Sepharose 2B-CL resulted in isolation of tightly bound DNA-protein complexes which did not dissociate in 8 M urea or 0.1% SDS. Subsequent elution of DNA-protein complexes on a hydroxylapatite column with a buffer containing 4 M guanidine hydrochloride and 5 M urea caused partial dissociation of the complexes. Electrophoretic analysis revealed essential changes in the composition of proteins DNA-protein complexes of hepatoma cells nuclear matrix during inhibition of DNA synthesis.
D000818:Animals; D034961:Antigens, Nuclear; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D002847:Chromatography, Agarose; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D003864:Depression, Chemical; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008297:Male; D009675:Novobiocin / Q000494:pharmacology*; D009698:Nucleoproteins / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains
D034961:Antigens, Nuclear; D004273:DNA, Neoplasm; D009698:Nucleoproteins; D009675:Novobiocin
Issledovanie reorganizatsii belkovogo sostava iadernogo matriksa kletok gepatomy pri ingibirovanii sinteza DNK novobiotsinom.