[Cryoprotective properties of the components of diluents for bull seminal fluid].
Studied were the protective qualities of lipovitellinine with regard to spermatozoa at cryoconservation. It was found that following centrifugation at 2000 G of the GH22L diluent for 20 min the electrophoretic investigations on Cellogel revealed a strong drop of lipovitellinine from 25.33 +/- 4.21 to 2.33 +/- 0.24 per cent. With the Nagaze-Niva diluent the drop was from 24.20 +/- 4.56 to 14.10 +/- 2.95 per cent. The changes in the percent ratio of the protein fractions in dilution of bull semen at 1 + 4 were shown to be considerable with the GH22L diluent after the separation of part of the lipovitellinine. With the Nagaze-Niva diluent these changes were negligible. The separation of part of the lipovitellinine with GH22L led to an essential drop of spermatozoid motility and of the heat resistance of semen following thawing. With the Nagaze-Niva diluent the changes in the qualities of spermatozoa were not substantial after separating part of the lipovitellinine. It was also found that the GH22L diluent produced a better cryoprotective effect on spermatozoa than the Nagaze-Niva diluent.
D000818:Animals; D002417:Cattle; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004527:Egg Proteins / Q000494:pharmacology; D015387:Egg Proteins, Dietary; D004588:Electrophoresis, Cellulose Acetate; D005615:Freezing; D008297:Male; D011506:Proteins / Q000032:analysis; D012661:Semen / Q000032:analysis / Q000187:drug effects*; D012662:Semen Preservation; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects
D003451:Cryoprotective Agents; D004527:Egg Proteins; D015387:Egg Proteins, Dietary; D011506:Proteins; C001549:lipovitellin
Krioprotektivni kachestva na niakoi sŭstavki na razrediteli za semenna technost ot bik.