title: Amino acid sequence of a cardiotoxin-like basic polypeptide (CLBP) with low cytotoxic activity isolated from the venom of the Formosan cobra (Naja naja atra).
abstract: A cardiotoxin-like basic polypeptide, designated as CLBP, was isolated from the venom of Naja naja atra by gel filtration on Sephadex G-50 followed by CM-cellulose chromatography. The cytotoxicity toward Yoshida sarcoma cells and lethal toxicity toward mice of CLBP were both one-order lower than those of cardiotoxins and cobrotoxin, respectively. CLBP is a single polypeptide consisting of 61 amino acid residues with four intramolecular disulfide linkages. The amino acid sequence of CLBP shows a high degree of homology with those of cardiotoxins from the same venom, but differs in the 19 to 23 positions.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004179:Cobra Cardiotoxin Proteins / Q000302:isolation & purification / Q000633:toxicity; D004546:Elapid Venoms / Q000302:isolation & purification / Q000633:toxicity; D007928:Lethal Dose 50; D051379:Mice; D010446:Peptide Fragments / Q000032:analysis; D010455:Peptides / Q000633:toxicity; D013329:Structure-Activity Relationship
chemical_list: D004179:Cobra Cardiotoxin Proteins; D004546:Elapid Venoms; D010446:Peptide Fragments; D010455:Peptides