S-antigen is coded by [poly A+] mRNA from bovine retina.
Total RNA, [poly (A)-] mRNA and [poly (A)+] mRNA purified from bovine retina were translated in vitro in a rabbit reticulocyte lysate system. Immunoprecipitation of translation products with antibodies to the retinal S-antigen (a photoreceptor specific protein involved in autoimmune retinal disease) revealed this protein as a 50,000 daltons band comigrating with purified S-antigen. This indicates that the S-antigen is synthesized in the retina and is not a maturation or degradation product of a larger protein. Its messenger RNA is the polyadenylated RNA, as for some other proteins expressed in nervous tissue.
D000818:Animals; D000941:Antigens / Q000096:biosynthesis* / Q000235:genetics; D019393:Arrestin; D002417:Cattle; D002474:Cell-Free System; D066298:In Vitro Techniques; D011061:Poly A / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D012160:Retina / Q000378:metabolism*
D000941:Antigens; D019393:Arrestin; D012333:RNA, Messenger; D011061:Poly A