IgE and IgGa antibody-mediated release of histamine from rat peritoneal cells. I. Optimum conditions for in vitro preparation of target cells with antibody and and challenge with antigen.
The optimum conditions for antigen-induced release of histamine in the rat IgE and IgGa antibody-mediated systems were studied in vitro. The IgE antibody-mediated reaction could be separated into two steps: preparation of target cells with antibody and challenge with antigen. The optimal conditions for these two steps were distinctly different. Release of histamine by IgGa antibody and antigen could not be separated into two steps, and the optimal conditions for the total reaction were identical to those of the antigen challenge step of the IgE antibody-mediated system.
D000818:Animals; D000906:Antibodies; D000937:Antigen-Antibody Reactions; D002118:Calcium / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D006636:Histamine Release; D006863:Hydrogen-Ion Concentration; D007111:Immunity, Cellular; D007074:Immunoglobulin G / Q000502:physiology*; D007136:Immunoglobulins / Q000502:physiology; D066298:In Vitro Techniques; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000276:immunology*; D009994:Osmolar Concentration; D010537:Peritoneum / Q000166:cytology / Q000276:immunology*; D051381:Rats; D014508:Urea / Q000494:pharmacology
D000906:Antibodies; D007074:Immunoglobulin G; D007136:Immunoglobulins; D014508:Urea; D006493:Heparin; D008274:Magnesium; D002118:Calcium