Isolation and characterization of a blood group active glycopeptide from porcine aorta.
A fucose-rich glycopeptide was prepared from the pronase digest of porcine thoracic aorta by gel-filtration through Sephadex G-100, DEAE-Sephadex A-25 column chromatography and alpha-amylase digestion. This glycopeptide was electrophoretically homogeneous. The large molecular size and chemical composition suggested that this glycopeptide was derived from mucin-type glycoprotein. The results of the beta-elimination reaction indicated that this glycopeptide contained the O-glycosidic linkages between galactosamine and serine/threonine. This glycopeptide exhibited blood group A and H activities. The present study revealed that the porcine thoracic aorta contains a blood group antigen of mucin-type glycoprotein nature.
D000818:Animals; D001013:Aorta, Thoracic / Q000032:analysis*; D001789:Blood Group Antigens / Q000276:immunology; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D006023:Glycoproteins / Q000302:isolation & purification*; D009077:Mucins; D013552:Swine / Q000097:blood*
D001789:Blood Group Antigens; D006023:Glycoproteins; D009077:Mucins