Solid-phase synthesis of 5'-phosphorylated oligodeoxynucleotides.
We used morpholino groups to protect phosphate during the phosphorylation of the 5'-terminal ends of oligodeoxynucleotides, via phosphotriester and phosphoramidite intermediates. These groups could be removed selectively.
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D010766:Phosphorylation
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides