A streamlined method of subfragment one preparation from myosin.
A rapid procedure for isolating subfragment one (SF1) from myosin was found. SF1 can be isolated specifically from proteolytic digests of myosin in the presence of a millimolar concentration of magnesium chloride. Under such ionic conditions all of the rod portion and undigested myosin is selectively precipitated. A nucleotide trapping experiment indicated how important quick preparation of SF1 is for maintaining the active site structure. This method can also be utilized in the preparation of heavy meromyosin.
D000244:Adenosine Diphosphate / Q000378:metabolism; D000818:Animals; D008274:Magnesium / Q000378:metabolism; D015879:Myosin Subfragments; D009218:Myosins / Q000302:isolation & purification* / Q000378:metabolism; D010446:Peptide Fragments / Q000302:isolation & purification* / Q000378:metabolism; D011817:Rabbits; D013997:Time Factors
D015879:Myosin Subfragments; D010446:Peptide Fragments; D000244:Adenosine Diphosphate; D009218:Myosins; D008274:Magnesium