title: Demonstration of beta-casomorphin immunoreactive materials in in vitro digests of bovine milk and in small intestine contents after bovine milk ingestion in adult humans.
abstract: Healthy young volunteers ingested one liter of cows' milk; then the contents of the small intestine were aspirated through an intestinal tube at various times and assayed for the presence of bovine beta-casomorphin immunoreactive materials. Considerable amounts of beta-casomorphin-7, but no beta-casomorphin-5 and only small amounts of beta-casomorphin-4 or -6 immunoreactive materials were found. Chromatographical characterization showed that most of the beta-casomorphin-7 immunoreactive material was not identical with beta-casomorphin-7, whereas the major part of the beta-casomorphin-4 or -6 immunoreactive materials might be identical with their corresponding beta-casomorphins. Analogous results were obtained for in vitro digestion of bovine milk which had been designed as a rough imitation of the gastrointestinal digestion process. A regulatory influence of beta-casomorphins as "food hormones" on intestinal functions is suggested.
mesh_terms: D000328:Adult; D000818:Animals; D002364:Caseins / Q000378:metabolism; D002417:Cattle; D002845:Chromatography; D004063:Digestion; D004723:Endorphins / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007421:Intestine, Small / Q000032:analysis*; D008297:Male; D008892:Milk / Q000378:metabolism*; D010446:Peptide Fragments; D011863:Radioimmunoassay
chemical_list: D002364:Caseins; D004723:Endorphins; D010446:Peptide Fragments; C041128:beta-casomorphin 7; C034812:beta-casomorphin 5; C021968:beta-casomorphins