Toxicity of methods of implant material sterilization on corneal endothelium.
The toxicity of different procedures utilized for the sterilization of intraocular implant material was assessed on the endothelium of organ-cultured porcine corneas. Polymethylmethacrylate lenses sterilized by treatment with sodium hydroxide (NaOH), ethylene oxide, formaldehyde, and gamma radiation were added to a culture medium containing normal porcine corneas. Considering the viability of endothelial cells, appearance of intracellular degenerative vacuoles, and denudation of corneal Descemet's membrane as criterion for the evaluation of toxicity of different methods of sterilization, the NaOH-treated lenses were found to be the least toxic to porcine corneal endothelium. Phase-contrast microscopy and vital staining of the endothelium permitted direct viewing of the endothelium aiding in the assessment of toxicity.
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003315:Cornea / Q000187:drug effects* / Q000528:radiation effects; D004727:Endothelium / Q000187:drug effects / Q000528:radiation effects; D005027:Ethylene Oxide / Q000633:toxicity; D005557:Formaldehyde / Q000633:toxicity; D005720:Gamma Rays / Q000009:adverse effects; D009924:Organ Culture Techniques; D012972:Sodium Hydroxide / Q000633:toxicity; D013242:Sterilization / Q000379:methods*; D013552:Swine
D005557:Formaldehyde; D012972:Sodium Hydroxide; D005027:Ethylene Oxide