[In vitro study of the fusion products and cell hybrids of mouse oocytes, zygotes and blastomeres].
Several variants of mouse embryos were received by polyethylene glycol-induced fusion of ovulated oocytes, blastomeres from various developmental stages, zygotes and their fragments. Cytological analysis and observation in culture have been carried out. The nuclear dynamics during the process of fusion, onset of mitosis in fusion products, their cleavage and initial morphogenetic processes in developing embryos have been followed. The possibility of directed constitution of embryos with varying quantitative and qualitative combination of nuclei and cytoplasm is, thus, shown. Some aspects of application of these models in studying the controlling mechanisms of early embryogenesis are considered.
D000818:Animals; D001757:Blastomeres / Q000166:cytology* / Q000187:drug effects; D002459:Cell Fusion / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D006822:Hybrid Cells / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects; D010060:Ovulation; D011092:Polyethylene Glycols / Q000494:pharmacology; D015053:Zygote / Q000166:cytology* / Q000187:drug effects
D011092:Polyethylene Glycols
Issledovanie in vitro produktov sliianiia i kletochnykh gibridov ootsitov, zigot i blastomerov mysheĭ.