[Acid glycosidases of normal cattle lymphocytes and in chronic lympholeukemia].
The activity of five acid glycosidases was determined in lymphocytes from normal animals and animals with chronic lymphocytic leukemia. It was shown that alpha-D-mannosidase activity in leukemic lymphocytes was 5 times lower (p less than less than 0.001) and alpha-D-glucosidase activity was 2 times lower (p less than 0.01) than in normal controls. The progress of the disease and the increase in leukocyte count were accompanied by the decrease in alpha-D-mannosidase activity. No differences have been found in alpha-D-mannosidase properties (thermostability, Km values, ZnSl2 activation) in normal and leukemic lymphocytes.
D000818:Animals; D002417:Cattle; D002469:Cell Separation; D006026:Glycoside Hydrolases / Q000097:blood*; D006863:Hydrogen-Ion Concentration; D007945:Leukemia, Lymphoid / Q000201:enzymology / Q000662:veterinary*; D008214:Lymphocytes / Q000201:enzymology*; D013379:Substrate Specificity
D006026:Glycoside Hydrolases
Kislye glikozidazy limfotsitov krupnogo rogatogo skota v norme i pri khronicheskom limfoleĭkoze.