Growth hormone effect on accumulation of arterial basement membrane-like material studied on rabbit aortic myomedial cell cultures.
The effect of human growth hormone on arterial basement membrane-like (BM) material was studied. BM-like material was obtained from the cell layer of cultured aortic myomedial cells using a sonication-differential centrifugation technique. After the addition of small amounts of growth hormone (1 ng/ml) to the cultures, we observed a 26% increased incorporation of amino acids into BM-like material (2p less than 0.005). However, further increase in the incorporation was not observed using either 3 ng or 10 ng growth hormone per ml. Growth hormone inhibited removal/degradation of BM-like material by 16% (2p less than 0.01). However, pinocytosis rate and activity of major lysosomal enzymes: cathepsin D, acid phosphatase and beta-N-acetyl-glucosaminidase were unchanged. Incorporation of glycosaminoglycans as evaluated by [35SO4]-labelling was reduced by 8% when cells were exposed to growth hormone (2p less than 0.01). The present study demonstrates an effect of growth hormone on the turnover and composition of BM-like material in cultured arterial myomedial cells.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology* / Q000187:drug effects; D001485:Basement Membrane / Q000166:cytology*; D002478:Cells, Cultured; D004705:Endocytosis / Q000187:drug effects; D006025:Glycosaminoglycans / Q000096:biosynthesis; D013006:Growth Hormone / Q000494:pharmacology*; D006801:Humans; D006867:Hydrolases / Q000378:metabolism; D007700:Kinetics; D008247:Lysosomes / Q000201:enzymology; D011817:Rabbits
D000596:Amino Acids; D006025:Glycosaminoglycans; D013006:Growth Hormone; D006867:Hydrolases