Amyloid enhancing factor in hamster.
An amyloid enhancing factor (AEF) was extracted from spleens and livers of casein-treated hamsters. It shortened the induction time of experimental amyloidosis in recipient hamsters from 14 days to 4 days. Sepharose-4B gel filtration resolved the extract into 4 different fractions, with molecular weights of: higher than IO(7) (Vo-fraction), about 280 000, 59 000 and 12 000 respectively. In all 4 fractions AEF was present, indicating that AEF is probably a low molecular weight substance that easily aggregates, or associates with other compounds present in the spleen extract. AEF was precipitable at 50% ammonium sulphate saturation. On anion exchange chromatography in PBS (pH 7.2) of this precipitate, AEF was found in the fraction eluting at high NaCl concentration. This fraction did not show any relation to hamster protein AA, IgG or albumin with double immunodiffusion. Ultraviolet absorption spectrophotometry indicated nucleotide-like material, suggesting AEF to be related to nucleoproteins.
D000645:Ammonium Sulfate; D000682:Amyloid / Q000378:metabolism; D000686:Amyloidosis / Q000209:etiology*; D000818:Animals; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006224:Cricetinae; D006023:Glycoproteins / Q000302:isolation & purification*; D007700:Kinetics; D008099:Liver / Q000032:analysis*; D008297:Male; D008647:Mesocricetus; D008970:Molecular Weight; D013154:Spleen / Q000032:analysis*
D000682:Amyloid; D006023:Glycoproteins; C046748:amyloid enhancing factor; D000645:Ammonium Sulfate