Interaction of DAPI with double-stranded ribonucleic acids.
The interaction of DAPI with natural and synthetic double-stranded polyribonucleotides was studied with different optical and calorimetric methods. The results were similar to those obtained previously with double-stranded polydeoxynucleotides, i.e. two interaction modes, the first of which shows high affinity for AU clusters and consequent strong fluorescence enhancement. The results suggest caution in the use of DAPI as selective fluorescent staining agent for DNA in the presence of RNA. A narrow groove binding model with hydrogen bonds between DAPI and AU pairs is proposed. An intercalation mechanism can be excluded because of the non planarity of DAPI molecule.
D002151:Calorimetry; D002942:Circular Dichroism; D006358:Hot Temperature; D007211:Indoles; D009691:Nucleic Acid Denaturation; D012330:RNA, Double-Stranded; D013056:Spectrophotometry, Ultraviolet
D007211:Indoles; D012330:RNA, Double-Stranded; C007293:DAPI