Glycoconjugate cytochemistry of the rat fundic gland using lectin/colloidal-gold conjugates and Lowicryl K4M. Helix pomatia lectin is a specific marker for mucous neck cells in fundic glands of the rat gastric mucosa.
The fundic gland of the rat stomach was studied using the low-temperature embedding resin Lowicryl K4M and postembedding staining with lectin/colloidal-gold (CG) conjugates. Intense labeling with Ricinus communis agglutinin I was observed not only in mucous-producing cells but also in parietal cells. In contrast, Helix pomatia agglutinin (HPA) only labeled mucous neck cells and intermediate cells between mucous neck cells and chief cells. The other epithelial cells present in the rat fundic gland showed virtually no reaction with this lectin. Our results indicate that HPA might be a marker lectin of mucous neck cells and their derivatives. The combination of embedding in the hydrophilic resin Lowicryl K4M and postembedding staining with lectin-CG conjugates provided satisfactory staining results, and made it possible to visualize the precise distribution of terminal glycoconjugates in intracellular components as well as on the plasma membrane.
D000818:Animals; D050260:Carbohydrate Metabolism; D004847:Epithelial Cells; D005260:Female; D005404:Fixatives; D005753:Gastric Mucosa / Q000378:metabolism*; D006046:Gold / Q000378:metabolism; D037102:Lectins / Q000378:metabolism*; D008297:Male; D008854:Microscopy, Electron / Q000379:methods*; D037121:Plant Lectins; D051381:Rats
D005404:Fixatives; C038095:Helix lectin; D037102:Lectins; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; D006046:Gold