Responses of renal and hepatic vitamin K dependent gamma-glutamyl carboxylase substrates to warfarin and vitamin K treatments.
A single intraperitoneal injection of warfarin (5 mg/kg) in the rat causes maximal accumulation of hepatic vitamin K dependent carboxylase substrate by 8 hr. In the kidney accumulation is slower with maximal amounts of the substrate appearing at about 16 hr. Vitamin K administered intravenously to warfarin-treated rats causes the complete disappearance of the hepatic substrate in 2 hr. In contrast, neither a single nor multiple injections of the vitamin decrease the renal substrate level by more than 30%. However, this decrease can be augmented by inhibition of protein synthesis with cycloheximide.
D000818:Animals; D019735:Carbon-Carbon Ligases; D003513:Cycloheximide / Q000494:pharmacology; D007668:Kidney / Q000201:enzymology / Q000648:ultrastructure; D008025:Ligases / Q000378:metabolism*; D008297:Male; D008861:Microsomes / Q000201:enzymology; D008862:Microsomes, Liver / Q000201:enzymology*; D011516:Prothrombin / Q000037:antagonists & inhibitors / Q000378:metabolism; D011517:Prothrombin Time; D051381:Rats; D014812:Vitamin K / Q000494:pharmacology*; D014859:Warfarin / Q000494:pharmacology*
D014812:Vitamin K; D014859:Warfarin; D011516:Prothrombin; D003513:Cycloheximide; D008025:Ligases; D019735:Carbon-Carbon Ligases; C020660:glutamyl carboxylase