1-hydroxypyrene in human urine after exposure to coal tar and a coal tar derived product.
A method for isolating 1-hydroxypyrene from urine is described. The presence of 1-hydroxypyrene in urine was identified by fluorescence excitation and emission scanning after HPLC-separation. 1-Hydroxypyrene could be detected in the urine of rats following oral administration of as little as 0.5 microgram pyrene. The dose-dependence of 1-hydroxypyrene in urine was evident after a wide range of pyrene dosing. After therapeutical coal tar treatment of dermatological patients the enhanced excretion of 1-hydroxypyrene was highly significant. Employees of a creosote impregnating plant showed an excretion pattern of 1-hydroxypyrene which could be related to their work. 1-Hydroxypyrene in urine of non-exposed people was very low, but detectable. It is suggested that the method reported is suitable for the assessment of uptake of man to pyrene, a compound that is commonly present in work environments which are associated with pollution of polycyclic aromatic hydrocarbons.
D000328:Adult; D000818:Animals; D003033:Coal Tar; D003407:Creosote; D004781:Environmental Exposure; D005260:Female; D006801:Humans; D008297:Male; D009153:Mutagens / Q000032:analysis*; D009790:Occupations; D011721:Pyrenes / Q000652:urine*; D051381:Rats; D011919:Rats, Inbred Strains; D014934:Wood
D009153:Mutagens; D011721:Pyrenes; D003033:Coal Tar; D003407:Creosote; C033146:1-hydroxypyrene