[Fragments of limited trypsinolysis of native alpha-actinin].
Incubation of native alpha-actinin with trypsin leads to the formation of intermediate fragments that are quite resistant to further enzyme action (M 98, 85, 80, 70, 64, 55, 38, 30, 15 kDa). Analysis of these fragments made it possible to elucidate the course of trypsinolysis and to localize the fragments in the polypeptide chain of alpha-actinin subunit.
D000185:Actinin / Q000032:analysis*; D000595:Amino Acid Sequence; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D066298:In Vitro Techniques; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis*; D011817:Rabbits; D014357:Trypsin
D010446:Peptide Fragments; D000185:Actinin; D014357:Trypsin
Fragmenty ogranichennogo tripsinoliza nativnogo alpha-aktinina.