Localization of oestradiol in the rat nasal mucosa.
The metabolism of 4-14C-oestradiol in the rodent respiratory tract has been investigated in vitro. Using thin-layer radiochromatography, at least 3 and 2 metabolites were found at incubation with slices from the rat olfactory mucosa and lung, respectively. Autoradiography of the nasal region of rats injected intravenously with 4-14C-oestradiol showed a high level of radioactivity in the mucosa covering the respiratory region, and in the subepithelial parts of the olfactory mucosa. Autoradiography of rat and mouse lung slices incubated with 4-14C-oestradiol showed that a selective localization of radioactivity occurred in the bronchial mucosa. The radioactivity in the mucosa of the respiratory tract was reversibly bound and could be extracted with organic solvents.
D000818:Animals; D001345:Autoradiography; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D008168:Lung / Q000378:metabolism; D008797:Metyrapone / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009297:Nasal Mucosa / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
D004958:Estradiol; D008797:Metyrapone