Urea synthesis in hepatocytes isolated from young and old rats.
Effects of glucagon and epinephrine stimulation on urea synthesis from lactate, pyruvate, NH4Cl, and ornithine in isolated rat hepatocytes from young and old rats were compared. It was found that the production of urea from those substrates was almost the same in both age groups. The concentrations of hormones that caused the maximum effect were the same for both groups of rats. The effectiveness of the stimulation of urea synthesis by glucagon and epinephrine was the same in hepatocytes isolated from young and old rats.
D000375:Aging; D000643:Ammonium Chloride / Q000378:metabolism; D000818:Animals; D004837:Epinephrine / Q000494:pharmacology; D005934:Glucagon / Q000494:pharmacology; D005943:Gluconeogenesis; D066298:In Vitro Techniques; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009952:Ornithine / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid; D051381:Rats; D011919:Rats, Inbred Strains; D014508:Urea / Q000096:biosynthesis*
D007773:Lactates; D011773:Pyruvates; D000643:Ammonium Chloride; D019344:Lactic Acid; D019289:Pyruvic Acid; D014508:Urea; D005934:Glucagon; D009952:Ornithine; D004837:Epinephrine