Biosynthesis of lipids during embryogenesis of Ascaris lumbricoides eggs.
Alteration in lipid metabolism of Ascaris lumbricoides eggs during development in either air or nitrogen gas was studied using acetate-1-14C and labeled fatty acids. It is thought that formation of 14C-lipids and 14C-palmitoleate from acetate-1-14C and palmitate-1-14C in developing eggs may be influenced by the concentration of molecular oxygen in the medium. Acetate-1-14C was incorporated into palmitate, palmitoleate and unsaturated 18-carbon acid of fatty acids in the eggs, but the incorporation into saturated 18-carbon acid was slight. Radioactive fatty acid methyl esters were major lipid component of the radioactive lipid classes in the first and second stage larvae incubated with palmitate-1-14C. Isopentadecanoic acid, which is a small component of fatty acids in Ascaris eggs, contains a high percentage of the total radioactivity in the second stage larvae incubated with acetate-1-14C under both air and nitrogen. It appears that a conversion of the fatty acid biosynthesis system may occur during development in to the second stage larvae.
D000085:Acetates / Q000378:metabolism; D000332:Aerobiosis; D000693:Anaerobiosis; D000818:Animals; D001200:Ascaris / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis / Q000096:biosynthesis*; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis; D007814:Larva / Q000378:metabolism; D008055:Lipids / Q000032:analysis / Q000096:biosynthesis*; D010063:Ovum / Q000378:metabolism; D010168:Palmitates / Q000378:metabolism
D000085:Acetates; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D008055:Lipids; D010168:Palmitates; C047140:isopentadecanoic acid