Evidence that transcription of the hexokinase gene is increased in a rapidly growing rat hepatoma.
The level of hexokinase-specific mRNA in rat liver and in rat ascites hepatoma (Zajdela) was assessed by translating free polysomes from both cell types in a reticulocyte lysate. Translation of hexokinase occurred with approximately a 10 fold higher frequency in tumor cells than in liver, suggesting that transcriptional activation is in part responsible for increased levels of hexokinase in tumor.
D000818:Animals; D002474:Cell-Free System; D006593:Hexokinase / Q000096:biosynthesis / Q000235:genetics*; D007120:Immunochemistry; D066298:In Vitro Techniques; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism; D012333:RNA, Messenger / Q000302:isolation & purification; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D012156:Reticulocytes / Q000378:metabolism; D014158:Transcription, Genetic
D012333:RNA, Messenger; D006593:Hexokinase