Separation of isomeric lysophospholipids by reverse phase HPLC.
A reverse-phase high performance liquid chromatography (HPLC) method was developed which resolved isomers of lysophosphatidylcholine (LPC) differing in the location of the aliphatic chain (sn-1 or sn-2 position) and the position (delta 6 or delta 9) or geometric configuration (cis or trans) of the olefin group in monounsaturated species. LPC isomers containing an acyl substituent at the sn-2 position eluted before their 1-acyl-sn-glycero-3-phosphocholine (1-acyl LPC) counterparts. The retention times of both the sn-1 and sn-2 isomers of monounsaturated species increased in the order delta 9-cis less than delta 9-trans less than delta 6-cis. The integrated ultraviolet absorbance (203 nm) in binary mixtures of the delta 9-cis and delta 6-cis 2-acyl lysophospholipid isomers correlated with the lipid phosphorus content of corresponding column eluates (r = 0.994). Thus, the present method will facilitate synthesis of isomerically pure diradylphospholipids by providing homogeneous lysophospholipid precursors and help simplify the quantitative analysis of unsaturated lysophospholipid species.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007536:Isomerism; D008244:Lysophosphatidylcholines / Q000302:isolation & purification*; D008246:Lysophospholipids; D010743:Phospholipids / Q000302:isolation & purification*; D013329:Structure-Activity Relationship; D013997:Time Factors
D008244:Lysophosphatidylcholines; D008246:Lysophospholipids; D010743:Phospholipids