Differential binding of chromosomal proteins HMG1 and HMG2 to superhelical DNA.
The binding of chromosomal proteins HMG1 and HMG2 to various DNA structures was examined by a nitrocellulose filter binding assay using a 32P labelled supercoiled plasmid. Binding assays and competition experiments indicated that HMG2 has a higher affinity than HMG1 for supercoiled DNA. Studies at various ionic strengths and pH values reveal differences in the interaction of the two proteins with DNA. The results suggest that HMG1 and HMG2 are involved in distinguishable cellular functions.
D001665:Binding Sites; D003101:Collodion; D004278:DNA, Superhelical / Q000378:metabolism*; D006609:High Mobility Group Proteins / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009690:Nucleic Acid Conformation; D010957:Plasmids; D011485:Protein Binding
D004278:DNA, Superhelical; D006609:High Mobility Group Proteins; D003101:Collodion