Rapid method for the isolation of lipoproteins from human serum by precipitation with polyanions.
Procedures are described for the isolation of lipoproteins from human serum by precipitation with polyanions and divalent cations. A mixture of low and very low density lipoproteins can be prepared without ultracentrifugation by precipitation with heparin and either MnCl(2) alone or MgCl(2) plus sucrose. In both cases the precipitation is reversible, selective, and complete. The highly concentrated isolated lipoproteins are free of other plasma proteins as judged by immunological and electrophoretic methods. The low density and very low density lipoproteins can then be separated from each other by ultracentrifugation. The advantage of the method is that large amounts of lipoproteins can be prepared with only a single preparative ultracentrifugation. Polyanions other than heparin may also be used; when the precipitation of the low and very low density lipoproteins is achieved with dextran sulfate and MnCl(2), or sodium phosphotungstate and MgCl(2), the high density lipoproteins can subsequently be precipitated by increasing the concentrations of the reagents. These lipoproteins, containing small amounts of protein contaminants, are further purified by ultracentrifugation at d 1.22. With a single preparative ultracentrifugation, immunologically pure high density lipoproteins can be isolated from large volumes of serum.
D000085:Acetates; D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D002118:Calcium / Q000494:pharmacology; D002482:Cellulose; D011232:Chemical Precipitation; D002712:Chlorides; D002784:Cholesterol / Q000097:blood; D002914:Chylomicrons / Q000302:isolation & purification; D003911:Dextrans; D003956:Dialysis; D004586:Electrophoresis; D005782:Gels; D006493:Heparin; D006736:Horses; D006801:Humans; D007106:Immune Sera; D007122:Immunoelectrophoresis; D008055:Lipids / Q000032:analysis; D008074:Lipoproteins / Q000097:blood / Q000302:isolation & purification*; D008274:Magnesium; D008345:Manganese; D008722:Methods; D010209:Paper; D010743:Phospholipids / Q000097:blood; D010772:Phosphotungstic Acid; D011817:Rabbits; D012964:Sodium; D013194:Staining and Labeling; D013213:Starch; D013395:Sucrose; D013464:Sulfuric Acids; D014461:Ultracentrifugation
D000085:Acetates; D001798:Blood Proteins; D002712:Chlorides; D002914:Chylomicrons; D003911:Dextrans; D005782:Gels; D007106:Immune Sera; D008055:Lipids; D008074:Lipoproteins; D010743:Phospholipids; D013464:Sulfuric Acids; D010772:Phosphotungstic Acid; D008345:Manganese; D013395:Sucrose; D002482:Cellulose; D013213:Starch; D006493:Heparin; D002784:Cholesterol; D012964:Sodium; D008274:Magnesium; D002118:Calcium