Carbamylation of proteins and sulfacetamide free fraction in serum in experimentally-induced high blood urea states.
The aims of this study were to investigate the time course of carbamylation of serum proteins in animals with experimental renal failure (RF) or with high blood urea levels without RF and finally, to document the effect of these states on the binding of sulfacetamide. RF (mean serum creatinine of 24.5 mg/dL and BUN 190 mg/dL) was originated by administrating i.v. uranyl nitrate. Uremia (mean BUN of 94 mg/dL and serum creatinine of 1.3 mg/dL) was induced by giving multiple doses of urea by gavage. RF was associated with a marked increase in the carbamylation of serum proteins and in the free fraction of sulfacetamide (Sff), but the degree of carbamylation did not correlate with Sff. Uremia without RF increased the carbamylation of serum proteins and in this case, the degree of carbamylation correlated with Sff. It is concluded that in vivo, carbamylation of serum proteins modifies, in a limited manner, drug binding to serum protein.
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D001806:Blood Urea Nitrogen; D002219:Carbamates / Q000378:metabolism*; D003404:Creatinine / Q000097:blood; D007676:Kidney Failure, Chronic / Q000378:metabolism; D008297:Male; D011506:Proteins / Q000378:metabolism*; D011817:Rabbits; D013409:Sulfacetamide / Q000097:blood / Q000378:metabolism*; D013997:Time Factors; D014511:Uremia / Q000378:metabolism*
D001798:Blood Proteins; D002219:Carbamates; D011506:Proteins; D013409:Sulfacetamide; D003404:Creatinine