Simple purification of aromatic L-amino acid decarboxylase from human pheochromocytoma using high-performance liquid chromatography.
We purified aromatic L-amino acid decarboxylase (AADC) homogeneously and rapidly from human pheochromocytoma using high-performance liquid chromatography. HPLC with gel permeation and hydrophobic columns was highly effective, and the entire purification could be finished within 3 days. Purified AADC showed a single band with an Mr of 50,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and decarboxylated L-3,4-dihydroxyphenylalanine, L-5-hydroxytryptophan, and L-threo-3,4-dihydroxyphenylserine (a synthetic precursor of natural norepinephrine). Amino acid analysis of purified AADC was performed.
D000310:Adrenal Gland Neoplasms / Q000201:enzymology*; D000596:Amino Acids / Q000032:analysis; D065105:Aromatic Amino Acid Decarboxylase Inhibitors; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000302:isolation & purification* / Q000378:metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010673:Pheochromocytoma / Q000201:enzymology*; D011732:Pyridoxal Phosphate / Q000494:pharmacology; D013379:Substrate Specificity
D000596:Amino Acids; D065105:Aromatic Amino Acid Decarboxylase Inhibitors; D011732:Pyridoxal Phosphate; D001142:Aromatic-L-Amino-Acid Decarboxylases