title: Regulation of liver sea bass pyruvate kinase by temperature, substrates and some metabolic effectors.
abstract: Pyruvate kinase of sea bass (Dicentrarchus labrax L.) shows positive cooperativity with respect to both substrates PEP and ADP. The temperature is a modulator of this activity, changing KS0.5 and Hill coefficient values for PEP. The enzyme shows alanine and ATP inhibition and F-1,6-P2 activation at 22 degrees C. F-1,6-P2 eliminates the effect of alanine but not that of ATP. These results could indicate a regulation of this enzyme by temperature and possess kinetic properties which are similar to that of L-type mammals.
mesh_terms: D000818:Animals; D005399:Fishes; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D011770:Pyruvate Kinase / Q000378:metabolism*; D012623:Seawater; D013379:Substrate Specificity; D013696:Temperature; D014018:Tissue Distribution
chemical_list: D011770:Pyruvate Kinase