Characterization and separation of viral DNA polymerase in mouse milk.
Two DNA polymerase with properties of viral RNA-directed DNA polymerase can be found in RIII mouse milk. One enzyme is the polymerase of type-C viruses; this enzyme prefers manganese to magnesium with poly(rA).oligo(dT) as synthetic template, is inhibited by specific sera, and has an apparent molecular weight of 70,000. Milk from BALB/c and NIH Swiss mice contain a vast predominance of this type-C enzyme. The other DNA polymerase from RIII mouse milk prefers magnesium to manganese, is not inhibited by type-C antipolymerase serum, and appears larger on gel chromatography than the type-C viral polymerase. Its presence in milk from RIII mice and absence from milk of mice with low content of mammary tumor virus correlates to the relative degree of type-B virus expression in these mice. The DNA polymerase of Mason-Pfizer monkey virus, isolated from a rhesus monkey breast tumor, also has a marked preference for magnesium with poly(rA).oligo(dT), is not immunologically related to primate type-C viruses, and appears larger than the gibbon type-C enzyme on gel chromatography.
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D002845:Chromatography; D005260:Female; D000882:Haplorhini; D007118:Immunoassay; D008251:Macaca; D008274:Magnesium; D008324:Mammary Tumor Virus, Mouse / Q000201:enzymology*; D008345:Manganese; D051379:Mice; D008892:Milk / Q000201:enzymology*; D009841:Oligonucleotides / Q000378:metabolism; D009858:Oncogenic Viruses / Q000201:enzymology*; D011119:Polynucleotides / Q000378:metabolism; D012328:RNA Viruses / Q000201:enzymology*; D012194:RNA-Directed DNA Polymerase / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D011925:Rauscher Virus / Q000201:enzymology; D012190:Retroviridae / Q000201:enzymology; D013698:Templates, Genetic; D013942:Thymine Nucleotides / Q000378:metabolism
D000227:Adenine Nucleotides; D009841:Oligonucleotides; D011119:Polynucleotides; D013942:Thymine Nucleotides; D008345:Manganese; D012194:RNA-Directed DNA Polymerase; D008274:Magnesium