Cobalt as a mitochondrial density marker in a study of cytoplasmic exchange during mating of Schizophyllum commune.
A slow in vivo uptake of cobalt from a growth medium resulted in an increase in density of mitochondria of Schizophyllum commune. Differential labeling of donor and resident mycelia, and subsequent analysis of resident mycelia surrounding donor implants, detected cobalt-dense mitochondria and demonstrated exchange of mitochondria after hyphal fusion. Transfer of mitochondria occurred in fully compatible, common-A, and common-AB matings, but was not detected in common-B matings of the tetrapolar Basidiomycete S. commune.
D001487:Basidiomycota / Q000166:cytology / Q000254:growth & development*; D002499:Centrifugation, Density Gradient; D003035:Cobalt; D003593:Cytoplasm / Q000502:physiology*; D006651:Histocytochemistry; D008928:Mitochondria; D012723:Sex; D013194:Staining and Labeling; D013385:Succinate Dehydrogenase / Q000032:analysis
D003035:Cobalt; D013385:Succinate Dehydrogenase