Dispersal of rabbit lung into individual viable cells: a new model for the study of lung metabolism.
This new enzymatic method disperses rabbit lung into morphologically intact, viable individual cells. The scattered cells constitute more than 50 percent of the original tissue. At least 90 percent of the cells exclude trypan blue from the nucleus. The dispersed lung cells consumed 6.2 microliters of oxygen per hour per milligram, dry weight. They incorporated [1-(14)C]palmitate into lecithin.
D000818:Animals; D002247:Carbon Isotopes; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D003851:Deoxyribonucleases / Q000378:metabolism; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D008722:Methods; D003012:Microbial Collagenase / Q000378:metabolism; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D010101:Oxygen Consumption; D010169:Palmitic Acids / Q000378:metabolism; D010196:Pancreatic Elastase / Q000378:metabolism; D010206:Papain / Q000378:metabolism; D010713:Phosphatidylcholines / Q000096:biosynthesis; D011402:Pronase / Q000378:metabolism; D011817:Rabbits; D013194:Staining and Labeling; D014343:Trypan Blue
D002247:Carbon Isotopes; D010169:Palmitic Acids; D010713:Phosphatidylcholines; D002374:Catalase; D003851:Deoxyribonucleases; D010196:Pancreatic Elastase; D010206:Papain; D011402:Pronase; D003012:Microbial Collagenase; D014343:Trypan Blue