Pyridine extraction of nocardial acid fastness.
Several strains of Mycobacterium and Nocardia were stained for acid fastness by using the method of Kinyoun. Duplicate, glutaraldehyde-fixed smears were extracted with fresh pyridine prior to staining. The results clearly demonstrated that both in vivo- and in vitro-grown Nocardia differed from Mycobacterium by becoming nonacid fast after extraction. This pyridine extraction of nocardial acid fastness should have diagnostic and taxonomic application.
D000143:Acids; D009161:Mycobacterium / Q000145:classification; D009615:Nocardia / Q000145:classification*; D011725:Pyridines; D012997:Solvents; D013045:Species Specificity; D013194:Staining and Labeling
D000143:Acids; D011725:Pyridines; D012997:Solvents