Adenosine 3'5'-monophosphate: inhibition of complement-mediated cell lysis.
An increase in adenosine 3',5'-monophosphate (cyclic AMP) in rat mast cells, achieved by stimulating the cells with prostaglandin E(1), by preventing cyclic AMP breakdown with aminophylline, or by adding exogenous dibutyryl cyclic AMP, prevented complement-mediated cytolysis as assessed by both histamine release and vital dye exclusion. Dibutyryl cyclic AMP also suppressed water-induced osmotic lysis.
D000628:Aminophylline / Q000494:pharmacology; D000818:Animals; D000937:Antigen-Antibody Reactions / Q000187:drug effects*; D003994:Bucladesine / Q000494:pharmacology; D003165:Complement System Proteins; D000242:Cyclic AMP / Q000096:biosynthesis / Q000494:pharmacology*; D003601:Cytotoxicity Tests, Immunologic; D006636:Histamine Release / Q000187:drug effects*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D066298:In Vitro Techniques; D008407:Mast Cells / Q000276:immunology* / Q000378:metabolism; D009996:Osmotic Fragility / Q000187:drug effects; D011453:Prostaglandins / Q000494:pharmacology; D011817:Rabbits / Q000276:immunology; D051381:Rats; D014343:Trypan Blue
D007166:Immunosuppressive Agents; D011453:Prostaglandins; D000628:Aminophylline; D003994:Bucladesine; D003165:Complement System Proteins; D000242:Cyclic AMP; D014343:Trypan Blue