Radioautography of the optic tectum of the goldfish after intraocular injection of ( 3 H)proline.
Radioautography of the optic tectum of the goldfish, performed after injection of [(3)H]proline into the contralateral eye, effectively resolves several distinct layers of retinal synapses. Silver grains are found unilaterally over nerve tracts containing efferent fibers from the tectum, a result that suggests intercellular migration of labeled molecules. The low background and high specific grain density obtained with [(3)H]proline radioautography indicate the usefulness of this technique for the elucidation of neuroanatomical connections in the visual system.
D000818:Animals; D001370:Axonal Transport; D003530:Cyprinidae; D005123:Eye; D007267:Injections; D011392:Proline / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism*; D013477:Superior Colliculi / Q000033:anatomy & histology / Q000378:metabolism*; D014316:Tritium
D014316:Tritium; D011392:Proline