Latency of human measles virus in hamster cells.
A latent system employing measles virus (Schwarz strain) was developed in hamster embryo fibroblasts (HEF). Measles virus-specific antigen was detected by immunofluorescence in 30 to 50% of HEF cells, and these cells released infectious virus when co-cultivated with a susceptible monkey cell line, BSC-1 cells. No infectious virus could be detected in the cells when measures were taken to exclude passage of viable latent cells onto the indicator BSC-1 cells. Infectious center assays demonstrated that about 1 in 10 of the latently infected cells in the population could release infectious virus. Infectious virus appeared within 6 hr after co-cultivation of the HEF cells with BSC-1 cells, as compared to 24 hr required for normal replication of measles virus in the BSC-1 cells. Furthermore, labeling of progeny virus ribonucleic acid (RNA) by using tritiated uridine, and inhibition of RNA or protein synthesis by 5-azacytidine or cycloheximide suggested that neither additional RNA nor protein synthesis is required after co-cultivation of the cells to effect early virus release. It can therefore be postulated that there is a block at a late step in virus replication in the latently infected hamster cells. The most obvious site would concern maturation of infectious virions at the cell membrane.
D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification; D001374:Azacitidine / Q000494:pharmacology; D002459:Cell Fusion; D002460:Cell Line; D002478:Cells, Cultured / Q000276:immunology / Q000382:microbiology*; D006224:Cricetinae; D003513:Cycloheximide / Q000494:pharmacology; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D000882:Haplorhini; D007668:Kidney; D008459:Measles virus / Q000254:growth & development* / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D009500:Neutralization Tests; D010222:Parainfluenza Virus 1, Human / Q000254:growth & development; D012367:RNA, Viral / Q000096:biosynthesis; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D010948:Viral Plaque Assay; D014779:Virus Replication
D000956:Antigens, Viral; D012367:RNA, Viral; D014316:Tritium; D003513:Cycloheximide; D001374:Azacitidine; D014529:Uridine