Mode of action of zorbamycin.
Zorbamycin (U-30,604E) induces rapid degradation of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) in Bacillus subtilis cells. DNA degradation is initiated first and is closely followed by the degradation of RNA. No interaction between isolated DNA and zorbamycin is observed. Nucleic acid and protein syntheses are not inhibited by zorbamycin in cell-free systems. Since the initial effect of the antibiotic is expressed at the level of the cellular DNA fraction, we assume that zorbamycin somehow induces a change in the structure or function of the cellular DNA fraction which results in rapid breakdown of this fraction.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001224:Aspartic Acid / Q000378:metabolism; D001412:Bacillus subtilis / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis; D001761:Bleomycin / Q000494:pharmacology; D002247:Carbon Isotopes; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D003300:Copper / Q000494:pharmacology; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis / Q000096:biosynthesis; D004926:Escherichia coli; D005815:Genetic Code; D005827:Genetics, Microbial; D009691:Nucleic Acid Denaturation; D010455:Peptides / Q000494:pharmacology; D011523:Protoplasts; D012329:RNA, Bacterial / Q000096:biosynthesis; D012270:Ribosomes / Q000187:drug effects; D013053:Spectrophotometry; D013395:Sucrose; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014498:Uracil / Q000378:metabolism
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002247:Carbon Isotopes; D004269:DNA, Bacterial; D010455:Peptides; D012329:RNA, Bacterial; D014316:Tritium; D001761:Bleomycin; D001224:Aspartic Acid; D014498:Uracil; D013395:Sucrose; D003300:Copper; D004254:DNA Nucleotidyltransferases; D013936:Thymidine