Colorimetric measurement of dermatan sulphate.
The periodate-Schiff reaction has been adapted for the measurement of dermatan sulphate. The method is specific for this glycosaminoglycan, provided that glycogen and glycoproteins are removed. Measurements of dermatan sulphate present in the urine of patients affected by various mucopolysaccharidoses indicate a good agreement between the values obtained with enzymic methods and those obtained with the colorimetric method described.
D002845:Chromatography; D003124:Colorimetry; D006003:Glycogen; D006023:Glycoproteins; D006025:Glycosaminoglycans / Q000032:analysis* / Q000652:urine; D006801:Humans; D008722:Methods; D009083:Mucopolysaccharidoses / Q000652:urine; D010504:Periodic Acid; D013194:Staining and Labeling
D006023:Glycoproteins; D006025:Glycosaminoglycans; D010504:Periodic Acid; D006003:Glycogen