title: New in-vitro test for IgE-mediated hypersensitivity in man.
abstract: A new simple and sensitive in-vitro method for the diagnosis of type 1 (IgE-mediated) hypersensitivity in man is described. Sliced human skin is passively sensitized by reaginic serum from allergic patients and the presence of antigen-specific IgE on the sensitized slices is detected by assay of antigen-evoked histamine release. Serum from 12 out of 14 patients with clinical respiratory allergy and positive skin tests gave significant antigen-specific histamine release. This method, which is essentially an in-vitro model of the Prausnitz-Küstner reaction, should prove of value in the diagnosis of human reaginic hypersensitivity in man.
mesh_terms: D000328:Adult; D000941:Antigens; D005260:Female; D006636:Histamine Release; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis*; D007073:Immunoglobulin E; D007136:Immunoglobulins; D066298:In Vitro Techniques; D008297:Male; D011058:Pollen; D012130:Respiratory Hypersensitivity / Q000175:diagnosis; D012867:Skin / Q000276:immunology; D012882:Skin Tests
chemical_list: D000941:Antigens; D007136:Immunoglobulins; D007073:Immunoglobulin E