Structural studies of avian myeloblastosis virus: comparison of polypeptides in virion and core component by dodecyl sulfate-polyacrylamide gel electrophoresis.
Two different systems of dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in separate laboratories detected analogous patterns of dye bands in virions of avian myeloblastosis virus (AMV). At least 11 of the dye bands co-migrated with the major polypeptides reported in Rous sarcoma virus. Particles with the morphology of the AMV core component, obtained after exposure of AMV to the nonionic surfactant Sterox SL, contained major polypeptides p12, p27, p60, p64, p91, and p98. The polypeptide p12 has been previously shown to be the major constituent of the inner ribonucleoprotein (RNP) of the AMV core, and has been designated p12(N). Two RNP polypeptides, p64 and p91, co-electrophoresed with purified AMV DNA polymerase and have now been designated p64(P) and p91(P). The polypeptide p27 has been identified as a probable constituent of the core shell, and has accordingly now been designated p27(C). In comparison to virions of AMV, the AMV core component contained a greatly reduced amount of polypeptide p15 and appeared to lack a major polypeptide, p19. Consequently, these polypeptides may be associated either with the exterior of the core shell or the interior of the viral envelope. Glycopeptides were not detected in AMV cores, in agreement with earlier reports that they reside in external projections from the viral envelope.
D001354:Avian Leukosis Virus / Q000032:analysis* / Q000201:enzymology; D001358:Avian Sarcoma Viruses / Q000032:analysis; D002250:Carbon Radioisotopes; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D004254:DNA Nucleotidyltransferases / Q000032:analysis; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D006020:Glycopeptides / Q000032:analysis; D006146:Guanidines; D008722:Methods; D008854:Microscopy, Electron; D008970:Molecular Weight; D009698:Nucleoproteins / Q000032:analysis* / Q000302:isolation & purification; D010455:Peptides / Q000032:analysis*; D010772:Phosphotungstic Acid; D012967:Sodium Dodecyl Sulfate; D013194:Staining and Labeling; D014764:Viral Proteins / Q000032:analysis* / Q000302:isolation & purification
D002250:Carbon Radioisotopes; D006020:Glycopeptides; D006146:Guanidines; D009698:Nucleoproteins; D010455:Peptides; D014764:Viral Proteins; D010772:Phosphotungstic Acid; D012967:Sodium Dodecyl Sulfate; D004254:DNA Nucleotidyltransferases