Nuclear disruption after infection of Escherichia coli with a bacteriophage T4 mutant unable to induce endonuclease II.
Nuclear disruption after infection of Escherichia coli with a bacteriophage T4 mutant deficient in the ability to induce endonuclease II indicates that either (i) the endonuclease II-catalyzed reaction is not the first step in host deoxyribonucleic acid (DNA) breakdown or (ii) nuclear disruption is independent of nucleolytic cleavage of the host chromosome. M-band analysis demonstrates that the host DNA remains membrane-bound after infection with either an endonuclease II-deficient mutant or T4 phage ghosts.
D002247:Carbon Isotopes; D002467:Cell Nucleus; D002499:Centrifugation, Density Gradient; D002876:Chromosomes, Bacterial; D003090:Coliphages / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis; D003673:Defective Viruses; D003851:Deoxyribonucleases / Q000096:biosynthesis*; D004790:Enzyme Induction; D004926:Escherichia coli / Q000166:cytology* / Q000378:metabolism; D005796:Genes; D005827:Genetics, Microbial; D007181:Inclusion Bodies, Viral; D008854:Microscopy, Electron; D009154:Mutation; D013194:Staining and Labeling; D013395:Sucrose; D013936:Thymidine / Q000378:metabolism; D014764:Viral Proteins / Q000494:pharmacology
D002247:Carbon Isotopes; D004269:DNA, Bacterial; D004279:DNA, Viral; D014764:Viral Proteins; D013395:Sucrose; D003851:Deoxyribonucleases; D013936:Thymidine