title: Serological analysis of the deoxyribonucleic acid polymerase of avian oncornaviruses. I. Preparation and characterization of monospecific antiserum with purified deoxyribonucleic acid polymerase.
abstract: Monospecific antiserum was prepared against purified deoxyribonucleic acid (DNA) polymerase from avian myeloblastosis virus (AMV). Immunodiffusion assay with purified DNA polymerase revealed that the anti-DNA polymerase serum formed one precipitation band, whereas no reaction with any of the seven major structural proteins of AMV was observed. The antiserum also demonstrated enzyme-neutralizing antibody activity that was associated with the immunoglobulin G fraction. There was no difference in the neutralization of DNA polymerase activity directed by ribonucleic acid (RNA), DNA, or RNA-DNA hybrid templates.
mesh_terms: D000818:Animals; D000956:Antigens, Viral; D001354:Avian Leukosis Virus / Q000201:enzymology / Q000276:immunology*; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D007106:Immune Sera; D007120:Immunochemistry; D005779:Immunodiffusion; D007074:Immunoglobulin G; D009500:Neutralization Tests; D012194:RNA-Directed DNA Polymerase / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats / Q000276:immunology; D013698:Templates, Genetic; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
chemical_list: D000956:Antigens, Viral; D007106:Immune Sera; D007074:Immunoglobulin G; D014316:Tritium; D012194:RNA-Directed DNA Polymerase; D013936:Thymidine