The glycoprotein of vesicular stomatitis virus is the antigen that gives rise to and reacts with neutralizing antibody.
The glycoprotein, but no other virion protein, of vesicular stomatitis virus was solubilized by the nonionic detergent Triton X-100 in low ionic strength buffer. The solubilized viral glycoprotein induced the synthesis of antibody that formed a single precipitin line with the glycoprotein and that neutralized the infectivity of the virus. The neutralizing activity of the antibody was efficiently blocked by purified glycoprotein.
D000596:Amino Acids; D000937:Antigen-Antibody Reactions; D000956:Antigens, Viral; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D005944:Glucosamine; D006023:Glycoproteins / Q000302:isolation & purification; D005779:Immunodiffusion; D009500:Neutralization Tests; D013501:Surface-Active Agents; D014316:Tritium; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology*
D000596:Amino Acids; D000956:Antigens, Viral; D002247:Carbon Isotopes; D000939:Epitopes; D006023:Glycoproteins; D013501:Surface-Active Agents; D014316:Tritium; D005944:Glucosamine