Stimulated normal human lymphocytes contain a ribonuclease-sensitive DNA polymerase distinct from viral RNA-directed DNA polymerase.
Ribonuclease-sensitive DNA synthesis is demonstrated in a cytoplasmic particulate fraction of normal human blood lymphocytes stimulated with phytohemagglutinin, but not in unstimulated lymphocytes. DNA polymerase purified from this fraction does not transcribe the heteropolymeric regions of 70S RNA from RNA tumor viruses, thus distinguishing this enzyme from the RNA-directed DNA polymerase (reverse transcriptase) found in oncogenic RNA viruses and human leukemic cells.
D002499:Centrifugation, Density Gradient; D003593:Cytoplasm / Q000201:enzymology; D004247:DNA / Q000032:analysis / Q000096:biosynthesis; D004254:DNA Nucleotidyltransferases / Q000302:isolation & purification* / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D006801:Humans; D037102:Lectins; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000201:enzymology*; D009693:Nucleic Acid Hybridization; D009711:Nucleotides / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase; D012260:Ribonucleases / Q000378:metabolism; D012293:Rifampin / Q000494:pharmacology; D013698:Templates, Genetic
D037102:Lectins; D009711:Nucleotides; D012367:RNA, Viral; D003609:Dactinomycin; D004247:DNA; D004254:DNA Nucleotidyltransferases; D012194:RNA-Directed DNA Polymerase; D012260:Ribonucleases; D012293:Rifampin