2,4-Dinitrophenyl receptors on mouse thymus and spleen cells.
A higher percentage of specific antigen-binding cells can be detected not only in normal CBA/J mouse spleen cell preparations (0.99%), but also in the normal thymus cell preparations (0.15%) with the use of [(125)I]2,4-dinitrophenyl-human IgG (DNP-HGG) as compared with most other antigens employed under similar conditions. The receptors on these cells are mainly specific for the DNP group as shown by the inhibition studies with DNP-lysine and the other DNP conjugates. In addition, it was shown by the inhibition studies with DNP-lysine that the thymus cells seem to have a lower avidity for DNP than the spleen cells. Preincubation of cell suspensions with antisera to immunoglobulins showed that the DNP-HGG antigen-binding cells in the thymus are inhibited predominantly with anti-micro-chain serum and the spleen cells with both anti-micro-chain and anti-gamma-chain sera; both cell populations were also significantly inhibited with the antisera to kappa-chains and Fab fragments. These data indicate that the nature of the receptor on the T cell differs from that on the majority of spleen cells.
D000818:Animals; D000941:Antigens; D001345:Autoradiography; D001665:Binding Sites; D003348:Cortisone / Q000494:pharmacology; D004140:Dinitrophenols; D007106:Immune Sera; D051379:Mice; D008808:Mice, Inbred CBA; D010047:Ovalbumin; D012710:Serum Albumin, Bovine; D013154:Spleen / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D005719:gamma-Globulins
D000941:Antigens; D004140:Dinitrophenols; D007106:Immune Sera; D005719:gamma-Globulins; D012710:Serum Albumin, Bovine; D010047:Ovalbumin; D003348:Cortisone