Cell-cycle analysis in 20 minutes.
Mithramycin added to mammalian cells fixed in aqueous ethanol is bound to DNA and fluoresces in direct proportion to the cellular DNA content. Quantitative fluorescence measurement by means of a high-speed flow system instrument provides a rapid method for cell-cycle analysis and for the first time permits continuous monitoring of cell-cycle kinetics during ongoing experiments.
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured / Q000032:analysis*; D006224:Cricetinae; D003584:Cytological Techniques; D004247:DNA / Q000032:analysis*; D004261:DNA Replication; D005260:Female; D005453:Fluorescence; D005470:Fluorometry; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008168:Lung / Q000196:embryology; D008722:Methods; D008938:Mitosis; D010053:Ovary; D008926:Plicamycin; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling
D004247:DNA; D008926:Plicamycin