DNA polymerase in virions of a reptilian type C virus.
A study was made of the DNA polymerase of reptilian type C virus isolated from Russell's viper spleen cells. Simultaneous detection experiments demonstrated the presence of 70S RNA and RNA-dependent DNA polymerase activity in reptilian type C virions. The endogenous activity was dependent on the addition of all four deoxynucleotide triphosphates and demonstrated an absolute requirement for a divalent cation. The reptilian viral DNA polymerase elutes from phosphocellulose at 0.22 M salt. In this respect, it is similar to the avian (avian myeloblastosis virus; AMV) viral enzyme but is different from the mammalian (Rauscher leukemia virus; RLV) viral enzyme which elutes at 0.4 M salt. The molecular weight of the viper DNA polymerase as estimated from glycerol gradient centrifugation is 109,000. It is a smaller enzyme than the AMV DNA polymerase (180,000 daltons) and somewhat larger than the RLV enzyme (70,000 daltons). A comparison of other properties of the type C reptilian DNA polymerase with the enzyme found in other type C oncogenic viruses is made.
D000818:Animals; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D002845:Chromatography; D004279:DNA, Viral / Q000096:biosynthesis; D008274:Magnesium / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000302:isolation & purification* / Q000378:metabolism; D012104:Reptiles; D012190:Retroviridae / Q000201:enzymology*; D013501:Surface-Active Agents; D013696:Temperature; D013698:Templates, Genetic; D013942:Thymine Nucleotides / Q000378:metabolism; D014316:Tritium
D004279:DNA, Viral; D013501:Surface-Active Agents; D013942:Thymine Nucleotides; D014316:Tritium; D008345:Manganese; D012194:RNA-Directed DNA Polymerase; D008274:Magnesium