Assignment of three human genes to chromosomes (LDH-A to 11, TK to 17, and IDH to 20) and evidence for translocation between human and mouse chromosomes in somatic cell hybrids (thymidine kinase-lactate dehydrogenase A-isocitrate dehydrogenase-C-11, E-17, and F-20 chromosomes).
Independently derived man-mouse somatic cell hybrids and their derivative subclones show a positive correlation between the expression of human lactate dehydrogenase A subunits and the occurrence of the human C-11 chromosome. Data are also presented that confirm the previously reported linkage of the thymidine kinase locus to the E-17 chromosome. A translocation of the E-17 chromosome provides presumptive evidence for the assignment of the thymidine kinase locus to the long arm segment of the E-17 chromosome. This translocation also provides evidence for translocation between man and mouse chromosomes in somatic cell hybrids. A presumptive association between the human phenotype for isocitrate dehydrogenase and the human F group is also described. Identification of specific human chromosomes was achieved by the application of several new cytological techniques: measurement of chromosome arm length, in situ annealing with mouse satellite complementary RNA, constitutive heterochromatin staining with Giemsa, and quinacrine mustard fluorochromatic staining.
D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations; D002902:Chromosomes, Human, 16-18 / Q000201:enzymology*; D002903:Chromosomes, Human, 19-20 / Q000201:enzymology*; D002906:Chromosomes, Human, 6-12 and X / Q000201:enzymology*; D002999:Clone Cells; D003238:Connective Tissue; D004171:Diploidy; D005347:Fibroblasts; D008040:Genetic Linkage; D006570:Heterochromatin; D006801:Humans; D006822:Hybrid Cells; D007521:Isocitrate Dehydrogenase; D007621:Karyotyping; D007770:L-Lactate Dehydrogenase; D008168:Lung; D051379:Mice; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D011796:Quinacrine; D013194:Staining and Labeling; D013937:Thymidine Kinase
D006570:Heterochromatin; D007770:L-Lactate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D013937:Thymidine Kinase; D011796:Quinacrine