title: Characterization of L-leucine-induced germination of Trichophyton mentagrophytes microconidia.
abstract: l-Leucine and several amino acids were effective germination inducers of microconidia of Trichophyton mentagrophytes. During germination, phase-darkening and swelling occurred concomitantly with the loss of resistance to heat and stain, reduction of dry weight and specific gravity, and development of active glucose utilization. Germination induced by l-leucine was significantly stimulated by a pretreatment of the spores with sublethal doses of heat. No nucleosides or nucleotides were stimulatory to the l-leucine-induced germination of the microconidia. d-Leucine was almost an equally effective germinant as its l form. No carbohydrates, salts, vitamins, or other compounds tested induced germination of the fungus spore. Other factors that affected l-leucine-induced germination of the microconidia included the concentration of leucine, the inoculum size of the spores, temperature, and pH. The anaerobic condition and the presence or absence of carbon dioxide had no significant effects on the germination. Short germ tubes usually developed when the germinated spores were further incubated either in the presence or absence of l-leucine. The cytological study of the germinating microconidia revealed that the fragmentation of lipid granules was the major structural change associated with the germination.
mesh_terms: D000596:Amino Acids / Q000494:pharmacology; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D003594:Cytoplasmic Granules; D003973:Diatrizoate; D004151:Dipeptides / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007930:Leucine / Q000494:pharmacology*; D008055:Lipids; D008858:Microscopy, Phase-Contrast; D010101:Oxygen Consumption; D013170:Spores / Q000254:growth & development*; D013172:Spores, Fungal / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D013194:Staining and Labeling; D013237:Stereoisomerism; D014249:Trichophyton / Q000166:cytology / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism
chemical_list: D000596:Amino Acids; D002247:Carbon Isotopes; D004151:Dipeptides; D008055:Lipids; D003973:Diatrizoate; D007930:Leucine; D005947:Glucose