Isolation and characterization of subacute sclerosing panencephalitis virus nucleocapsids.
Nucleocapsids from subacute sclerosing panencephalitis (SSPE) virus-infected CV-1 cells were concentrated by differential centrifugation employing sucrose cushion techniques and further purified by centrifugation through a linear CsCl density gradient. The bouyant density of (3)H-uridine-labeled nucleocapsids in CsCl was found to be 1.31 g/cm(3). Ultraviolet absorption spectra of the purified SSPE nucleocapsid showed an absorption maximum at 260 to 265 nm and a 280/260 ratio that corresponded to a nucleic acid content of approximately 4.3%. Negatively stained preparations of SSPE nucleocapsids were found to have a width of 18 +/- 1 nm, a periodicity of 5 to 6 nm, and a length between 1-1.4 mum, with the greatest number at 1.3 mum.
D000818:Animals; D002247:Carbon Isotopes; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D003588:Cytopathogenic Effect, Viral; D003609:Dactinomycin / Q000494:pharmacology; D000882:Haplorhini; D007668:Kidney; D008854:Microscopy, Electron; D009698:Nucleoproteins / Q000302:isolation & purification; D012328:RNA Viruses / Q000032:analysis* / Q000254:growth & development; D013056:Spectrophotometry, Ultraviolet; D013194:Staining and Labeling; D013344:Subacute Sclerosing Panencephalitis / Q000382:microbiology*; D014316:Tritium; D014764:Viral Proteins / Q000302:isolation & purification; D014779:Virus Replication
D002247:Carbon Isotopes; D009698:Nucleoproteins; D014764:Viral Proteins; D014316:Tritium; D003609:Dactinomycin