The transporting proteins of cholecalciferol and 25-hydroxycholecalciferol in serum of chicks and other species. Partial purification and characterization of the chick proteins.
Chick serum contains two cholecalciferol-binding proteins, one of which binds mainly cholecalciferol (cholecalciferol-binding protein) and the other binds 25-hydroxycholecalciferol (25-hydroxycholecalciferol-binding protein). By means of Cohn fractionation, (NH(4))(2)SO(4) precipitation, gel filtration on Sephadex G-200, ion-exchange chromatography on DEAE-Sephadex and an additional gel-filtration step on Sephadex G-100, these two binding proteins were purified. Both proteins possess beta-globulin mobility on analytical polyacrylamide-disc-gel electrophoresis, a sedimentation coefficient of 3.5S and approximate molecular weights of 60000 for the cholecalciferol-binding protein and 54000 for the 25-hydroxycholecalciferol-binding protein. Sera obtained from rat, pig, human and monkey were shown to contain a single binding protein that is responsible for the transport of both cholecalciferol and 25-hydroxycholecalciferol. In the toad the lipoproteins are used for the transport of these two steroids.
D000645:Ammonium Sulfate; D000818:Animals; D001001:Anura; D001609:Beta-Globulins; D001797:Blood Protein Electrophoresis; D001798:Blood Proteins / Q000302:isolation & purification*; D002352:Carrier Proteins / Q000302:isolation & purification*; D011232:Chemical Precipitation; D002623:Chemistry Techniques, Analytical; D002645:Chickens; D002762:Cholecalciferol / Q000097:blood / Q000378:metabolism*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D000882:Haplorhini; D006801:Humans; D006887:Hydroxycholecalciferols / Q000097:blood / Q000378:metabolism*; D008074:Lipoproteins / Q000378:metabolism; D008970:Molecular Weight; D011485:Protein Binding; D051381:Rats; D013552:Swine
D001609:Beta-Globulins; D001798:Blood Proteins; D002352:Carrier Proteins; D006887:Hydroxycholecalciferols; D008074:Lipoproteins; D002762:Cholecalciferol; D000645:Ammonium Sulfate