Specificities of haemagglutinating antibodies evoked by members of the cephalosporin C family and benzylpenicillin.
1. Antisera have been produced in rabbits to benzylpenicillin and four members of the cephalosporin C family and to conjugates of these substances with bovine gamma-globulin. 2. Deacetoxycephalosporin C reacted less readily and deacetylcephalosporin C lactone more readily with bovine gamma-globulin than did benzylpenicillin, cephalosporin C or deacetylcephalosporin C. 3. Antisera to free or conjugated benzylpenicillin agglutinated red cells sensitized with a variety of penicillins, but only reacted to a significant extent with cells sensitized with the cephalosporins tested when the latter contained an N-phenylacetyl or chemically related side chain. 4. Antisera to members of the cephalosporin C family agglutinated cells sensitized with these cephalosporins or with penicillin N, but did not react with cephalosporins whose side chains were chemically unrelated to alpha-aminoadipic acid. 5. Members of the cephalosporin C family and products of hydrolysis of cephalosporin C behaved as hapten inhibitors of antisera to cephalosporin C, but 7-aminocephalosporanic acid was relatively ineffective. 6. These findings are discussed in relation to differences in the chemical properties of penicillins and cephalosporins.
D000818:Animals; D000918:Antibody Specificity; D001665:Binding Sites; D002417:Cattle; D002511:Cephalosporins; D003429:Cross Reactions; D004912:Erythrocytes / Q000032:analysis; D006241:Haptens; D006386:Hemagglutination Tests; D007106:Immune Sera; D008956:Models, Chemical; D010400:Penicillin G; D011817:Rabbits; D005719:gamma-Globulins
D002511:Cephalosporins; D006241:Haptens; D007106:Immune Sera; D005719:gamma-Globulins; D010400:Penicillin G