title: Observations on interactions between fluorescein-iso-thiocyanate conjugates and cells in vitro.
abstract: During the course of some routine immunofluorescence studies ;non-specific' staining occurred in the absence of free fluorescein and of any known impurities. The cause of such staining seems to be associated with phasic changes occurring at varying population doublings (;passages') in species-homologous cells subjected to serial cultivation.
mesh_terms: D000328:Adult; D003470:Culture Media; D046508:Culture Techniques; D005260:Female; D005347:Fibroblasts; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D017955:Poliovirus; D012672:Semliki forest virus; D013194:Staining and Labeling; D013861:Thiocyanates; D014616:Vaccinia virus
chemical_list: D003470:Culture Media; D005452:Fluoresceins; D013861:Thiocyanates