Morphology and function of cells of human embryonic liver in monolayer culture.
A system for culturing human fetal liver cells in monolayers is described and the effects of various conditions of growth on the morphology and function of the cultured cells are presented. The addition of 10% calf serum or 1% human serum to the growth medium accelerated the proliferation of the liver cells, with subsequent loss of characteristic morphology and specific functional activity. In the absence of serum, the cultured liver cells retained their morphology and their function for at least 4 wk, as evidenced by secretion of serum albumin and storage of glycogen and iron.
D003470:Culture Media; D046508:Culture Techniques; D005333:Fetus / Q000166:cytology; D005615:Freezing; D006801:Humans; D008099:Liver / Q000166:cytology / Q000196:embryology*; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D013194:Staining and Labeling; D013997:Time Factors; D014021:Tissue Preservation; D014357:Trypsin
D003470:Culture Media; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D014357:Trypsin