title: The glycoprotein of vesicular stomatitis virus is the antigen that gives rise to and reacts with neutralizing antibody.
abstract: The glycoprotein, but no other virion protein, of vesicular stomatitis virus was solubilized by the nonionic detergent Triton X-100 in low ionic strength buffer. The solubilized viral glycoprotein induced the synthesis of antibody that formed a single precipitin line with the glycoprotein and that neutralized the infectivity of the virus. The neutralizing activity of the antibody was efficiently blocked by purified glycoprotein.
mesh_terms: D000596:Amino Acids; D000937:Antigen-Antibody Reactions; D000956:Antigens, Viral; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D005944:Glucosamine; D006023:Glycoproteins / Q000302:isolation & purification; D005779:Immunodiffusion; D009500:Neutralization Tests; D013501:Surface-Active Agents; D014316:Tritium; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology*
chemical_list: D000596:Amino Acids; D000956:Antigens, Viral; D002247:Carbon Isotopes; D000939:Epitopes; D006023:Glycoproteins; D013501:Surface-Active Agents; D014316:Tritium; D005944:Glucosamine