Use of antiviral-antiferritin hybrid antibody for localization of viral antigen in plasma membrane.
Reduced Fab' fragments of viral antibody hybridized with reduced Fab' fragments of antiferritin immunoglobulin G bind to viral antigenic sites in the plasma membrane of L cells infected with vesicular stomatitis virus. The hybrid antibody reacts specifically with ferritin, which can be identified by electron microscopy, and with fluorescein-conjugated apoferritin, which can be identified by fluorescence microscopy.
D000818:Animals; D000906:Antibodies; D000941:Antigens / Q000302:isolation & purification*; D002462:Cell Membrane / Q000276:immunology*; D005293:Ferritins; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D007120:Immunochemistry; D007074:Immunoglobulin G; D007739:L Cells / Q000276:immunology*; D008722:Methods; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D011817:Rabbits; D013194:Staining and Labeling; D013997:Time Factors; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology* / Q000472:pathogenicity
D000906:Antibodies; D000941:Antigens; D007106:Immune Sera; D007074:Immunoglobulin G; D005293:Ferritins