Immunity tolerance to a hapten (NIP) coupled to an isologous carrier (mouse gamma globulin).
A hapten, 4-hydroxy-3-iodo-5-nitrophenylacetic acid (NIP) when coupled to isologous mouse gamma globulin (MgammaG) elicits a hapten-specific immune response in mice if administered in Freund's complete adjuvant. This response is measurable by the capacity of the sera to bind N(125)IP, by detection of NIP-specific plaque-forming cells (B cells), and by in vitro secondary type antigen-driven DNA synthesis (T cells and probably B cells). The in vitro response requires both the hapten and carrier since neither by itself is capable of stimulating the spleen cells. This same antigen gives rise to hapten-specific tolerance when given in the soluble form. Mice pretreated with soluble NIP-MgammaG and challenged with NIP coupled to a heterologous carrier give a normal antibody response to the carrier but have barely detectable levels of antibody to NIP. Spleen cells from mice made tolerant to NIP-MgammaG do not respond in vitro with increased DNA synthesis. This implies that thymus-derived cells as well as bone marrow-derived cells are involved in hapten-specific tolerance.
D000818:Animals; D000918:Antibody Specificity; D000921:Antibody-Producing Cells; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D004247:DNA / Q000096:biosynthesis; D005620:Freund's Adjuvant; D006241:Haptens; D007108:Immune Tolerance; D007109:Immunity; D007074:Immunoglobulin G; D066298:In Vitro Techniques; D051379:Mice; D008815:Mice, Inbred Strains; D010648:Phenylacetates; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D013950:Thymus Gland / Q000276:immunology; D014316:Tritium; D005719:gamma-Globulins
D006241:Haptens; D007074:Immunoglobulin G; D010648:Phenylacetates; D005719:gamma-Globulins; D014316:Tritium; D004247:DNA; D005620:Freund's Adjuvant; D013936:Thymidine