Regulation of subunit synthesis of Escherichia coli RNA polymerase.
lambdacI857s7drif(d)18 phage is a defective, specialized transducing phage carrying the RNA polymerase mutation rif(d), a dominant rifampicin-resistant allele. This phage DNA directs in vitro synthesis of the beta subunit of RNA polymerase in a DNA-dependent protein-synthesizing system. Prophage induction in cells that are lysogenic for this phage increases the rate of beta-subunit synthesis in vivo by at most 2-fold. No increase in subunit synthesis above the haploid level is observed in cells diploid for the beta gene.
D002250:Carbon Radioisotopes; D002474:Cell-Free System; D003090:Coliphages / Q000032:analysis; D004279:DNA, Viral / Q000302:isolation & purification / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000032:analysis / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D004926:Escherichia coli / Q000201:enzymology*; D005827:Genetics, Microbial; D007118:Immunoassay; D007930:Leucine / Q000378:metabolism; D011233:Precipitin Tests; D011392:Proline / Q000378:metabolism; D013698:Templates, Genetic; D014316:Tritium
D002250:Carbon Radioisotopes; D004279:DNA, Viral; D000939:Epitopes; D014316:Tritium; D011392:Proline; D012321:DNA-Directed RNA Polymerases; D007930:Leucine