Applications of a synthetic neuraminidase substrate.
A rapid and precise assay for neuraminidase using 2-(3'-methoxyphenyl)-N-acetyl-alpha-neuraminic acid (MPN) is described. It is proposed that this substrate be used for the standardization of activity of neuraminidases from viral, bacterial, and mammalian sources. MPN is also used as a chromogenic substrate to localize influenza and parainfluenza virus foci in tissue culture. This technique permits the recovery of infective virus from these stained "plaques." It has also been demonstrated that immunoprecipitin lines containing neuraminidase complexes with antibody in the Ouchterlony test can be observed by a similar staining procedure. No enzyme inhibition occurs in the presence of anti-neuraminidase antibodies or concanavalin A when MPN is used as a substrate in contrast to the results with high-molecular-weight substrates such as fetuin.
D000818:Animals; D002417:Cattle; D002460:Cell Line; D055598:Chemical Phenomena; D002621:Chemistry; D003208:Concanavalin A; D003470:Culture Media; D003588:Cytopathogenic Effect, Viral; D005326:Fetal Proteins; D007106:Immune Sera; D005779:Immunodiffusion; D007668:Kidney; D008722:Methods; D009438:Neuraminic Acids; D009439:Neuraminidase / Q000032:analysis*; D009975:Orthomyxoviridae / Q000201:enzymology / Q000276:immunology / Q000302:isolation & purification; D010636:Phenols; D011817:Rabbits / Q000276:immunology; D018108:Respirovirus / Q000201:enzymology / Q000276:immunology / Q000302:isolation & purification; D013194:Staining and Labeling; D010948:Viral Plaque Assay; D014779:Virus Replication
D003470:Culture Media; D005326:Fetal Proteins; D007106:Immune Sera; D009438:Neuraminic Acids; D010636:Phenols; D003208:Concanavalin A; D009439:Neuraminidase