Interaction of Pseudomonas bacteriophage 2 with the slime polysaccharide and lipopolysaccharide of Pseudomonas aeruginosa strain B1.
Purified slime polysaccharide B and lipopolysaccharide of Pseudomonas aeruginosa strain BI were shown to possess receptor-like properties in inactivating Pseudomonas phage 2, whereas lipoprotein and glycopeptide fractions were devoid of activity. On a weight basis, slime polysaccharide B was more effective than lipopolysaccharide in inactivating phage. The specificity of the reaction with slime polysaccharide B was indicated by the fact that slime polysaccharide A of P. aeruginosa strain EI failed to inactivate phage 2. Electron micrographs showed phage 2 in typical, tail-first position of attachment on intact cells of strain BI, slime polysaccharide B, and lipopolysaccharide. Tail fibers were discernible during phage attachment.
D000327:Adsorption; D001435:Bacteriophages / Q000187:drug effects; D002458:Cell Fractionation; D002855:Chromatography, Thin Layer; D006020:Glycopeptides / Q000302:isolation & purification / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000302:isolation & purification / Q000494:pharmacology; D008074:Lipoproteins / Q000302:isolation & purification / Q000494:pharmacology; D008854:Microscopy, Electron; D010772:Phosphotungstic Acid; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification / Q000494:pharmacology; D011550:Pseudomonas aeruginosa; D013045:Species Specificity; D013194:Staining and Labeling
D006020:Glycopeptides; D008070:Lipopolysaccharides; D008074:Lipoproteins; D011135:Polysaccharides, Bacterial; D010772:Phosphotungstic Acid