Characterization of a new murine cellular DNA polymerase.
A new DNA polymerase (peak A) has been identified in the high-speed pellet fraction of two subclones of nonvirus producing Balb/3T3 cells. The activity is associated with a molecule of approximately 70,000 molecular weight and chromatographs in two systems like the mouse type-C viral reverse transcriptase and a similar enzyme from the high-speed pellet fraction of a virus producing Balb/3T3 subclone, S(2)Cl(3). Comparable quantities of peak A are present in both nonvirus infected (A31) and mouse sarcoma virus transformed nonproducer (KA31) subclones. Virus producing cells contain 10-20 times more peak A polymerase activity. A31 and KA31 peak A are comparably inhibited by anti-mouse type C virus reverse transcriptase IgG but to a lesser degree than S(2)Cl(3) peak A or authentic viral reverse transcriptase. They can also be differentiated from the latter two enzymes by template preference studies. KA31 peak A can be distinguished from three other KA31 DNA polymerases (R-DNA polymerase and DNA polymerase N and C), and thus appears to be a new species of cellular DNA polymerase.
D000227:Adenine Nucleotides; D000818:Animals; D002499:Centrifugation, Density Gradient; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D002999:Clone Cells / Q000201:enzymology*; D003597:Cytosine Nucleotides; D004247:DNA; D004254:DNA Nucleotidyltransferases / Q000032:analysis / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism; D006150:Guanine Nucleotides; D007118:Immunoassay; D008274:Magnesium; D008345:Manganese; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D011119:Polynucleotides; D012194:RNA-Directed DNA Polymerase / Q000032:analysis / Q000378:metabolism; D011925:Rauscher Virus / Q000201:enzymology; D012190:Retroviridae / Q000201:enzymology; D013698:Templates, Genetic; D013942:Thymine Nucleotides
D000227:Adenine Nucleotides; D003597:Cytosine Nucleotides; D006150:Guanine Nucleotides; D011119:Polynucleotides; D013942:Thymine Nucleotides; D008345:Manganese; D004247:DNA; D004254:DNA Nucleotidyltransferases; D012194:RNA-Directed DNA Polymerase; D008274:Magnesium