title: Structural changes in Clostridium botulinum type E after treatment with boticin S5 1 .
abstract: Treatment with boticin S5(1), a bacteriocin produced by a nontoxigenic organism closely related to Clostridium botulinum type E, caused extensive changes in the structure of a sensitive C. botulinum type E strain. Nucleoid deoxyribonucleic acid, normally seen as fine filaments scattered throughout the cell, was aggregated into dense deoxyribonucleic acid masses. Mesosomes appeared to undergo structural rearrangement from lamellar to vesicular configuration. Eventual dissolution of cell contents left bacterial ghosts composed of seemingly intact cell walls with remnants of the cytoplasmic membrane and internal structures. The morphological changes observed in boticin-treated strain 070 cells were very similar to those produced by a bacteriocin-like substance from phage type 71 Staphylococcus aureus on sensitive beta-hemolytic streptococci. A similarity in the mode of action of the two bactericidal agents is suggested.
mesh_terms: D001430:Bacteriocins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects; D002473:Cell Wall; D003014:Clostridium botulinum / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D003470:Culture Media; D003593:Cytoplasm / Q000187:drug effects; D004269:DNA, Bacterial; D008854:Microscopy, Electron; D008970:Molecular Weight; D012703:Serotyping; D013194:Staining and Labeling
chemical_list: D001430:Bacteriocins; D003470:Culture Media; D004269:DNA, Bacterial