Characterization of yolk DNA from Xenopus laevis oocytes ovulated in vitro.
High molecular weight DNA was isolated from the yolk platelets of Xenopus laevis oocytes ovulated in vitro. Yolk DNA has the same buoyant density in CsCl gradients as mitochondrial and nuclear DNAs, and, like them, it is double-stranded. Yolk DNA behaves like nuclear DNA, and not like mitochondrial DNA, upon renaturation after denaturation. The molecules are always linear. Cytochemical and biochemical controls preclude the possibility that the yolk DNA might be contaminated by nuclear or mitochondrial DNA. We conclude that the yolk DNA is an endogenous component of the yolk platelets.
D000557:Ambystoma / Q000196:embryology; D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000032:analysis* / Q000378:metabolism; D003609:Dactinomycin / Q000378:metabolism; D005260:Female; D006651:Histocytochemistry; D007668:Kidney / Q000166:cytology; D008854:Microscopy, Electron; D008928:Mitochondria / Q000032:analysis; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009695:Nucleic Acid Renaturation; D010063:Ovum / Q000032:analysis* / Q000378:metabolism; D011485:Protein Binding; D014316:Tritium; D014461:Ultracentrifugation; D014981:Xenopus / Q000196:embryology*
D014316:Tritium; D003609:Dactinomycin; D004247:DNA