Studies on sex pili: mutants of the sex factor F in Escherichia coli defective in bacteriophage-adsorbing function of F pili.
Ultraviolet irradiation or nitrosoguanidine treatment of Escherichia coli K-12 strain JE3100 (F'(8)/fla pil) led to the isolation of six mutants defective in F pili function. The defects were shown to be caused by mutations in the F factor. The mutants retained conjugal fertility, although they were less efficient than parental F'(8) strain, and continued to synthesize F pili. Three of the mutants (strains KE196, 198, and 200) had lost sensitivity to male-specific MS2 phage, and the other three (strains KE161, 163, and 164) were insensitive to Qbeta and f1 as well as MS2 phages. F pili on strains KE196, 198, and 200 cells continued to adsorb MS2 phage, whereas those of strains KE161, 163, and 164 did not adsorb MS2 phage. The correlation of the mutant phenotypes with those of other F mutants reported in the literature is discussed.
D000166:Acridines / Q000494:pharmacology; D000327:Adsorption; D003090:Coliphages / Q000254:growth & development; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000166:cytology* / Q000187:drug effects / Q000302:isolation & purification / Q000528:radiation effects; D005111:Extrachromosomal Inheritance; D008242:Lysogeny; D008297:Male; D008854:Microscopy, Electron; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation; D009604:Nitrosoguanidines / Q000494:pharmacology; D010759:Phosphorus Isotopes; D011830:Radiation Effects; D013194:Staining and Labeling; D014466:Ultraviolet Rays; D014779:Virus Replication
D000166:Acridines; D009153:Mutagens; D009604:Nitrosoguanidines; D010759:Phosphorus Isotopes