Studies on the nucleic acid sequences of Kirsten sarcoma virus: a model for formation of a mammalian RNA-containing sarcoma virus.
The genetic information contained in the Kirsten and Moloney strains of mammalian RNA-containing sarcoma viruses has been analyzed by RNA . (3)H-DNA hybridization. Kirsten sarcoma virus has been found to possess two distinct sets of nucleic acid sequences. One set of sequences is contained in murine type C helper virus, and the other set is contained in rat type C helper virus. Moloney sarcoma virus contains sequences of murine type C helper virus but not of rat type C helper virus. The results indicate that Kirsten sarcoma virus arose through a process of recombination between Kirsten murine leukemia virus and nucleic acid sequences found in rat cells. A model is suggested for the formation of transforming type C viruses involving the transduction of oncogenic information.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004279:DNA, Viral / Q000096:biosynthesis*; D006378:Helper Viruses / Q000032:analysis; D009052:Leukemia Virus, Murine / Q000032:analysis*; D051379:Mice; D008954:Models, Biological; D008979:Moloney murine leukemia virus / Q000032:analysis* / Q000254:growth & development; D009693:Nucleic Acid Hybridization; D010761:Phosphorus Radioisotopes; D012367:RNA, Viral / Q000032:analysis*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D051381:Rats; D011995:Recombination, Genetic; D012190:Retroviridae / Q000032:analysis / Q000254:growth & development*; D014161:Transduction, Genetic; D014316:Tritium; D014776:Virus Cultivation
D004279:DNA, Viral; D010761:Phosphorus Radioisotopes; D012367:RNA, Viral; D014316:Tritium; D012194:RNA-Directed DNA Polymerase