title: The direct fluorescent antibody test for detection of Brucella abortus in bovine abortion material.
abstract: The direct fluorescent antibody test was assessed for detection of Brucella abortus in bovine abortion material. Br. abortus organisms could be readily detected as characteristic intra-cellular clumps, in smears stained with fluorescent antibody. Counterstaining with Evans Blue was necessary to suppress tissue auto-fluorescence. The method was specific and enabled brucella infection to be differentiated, inter alia, from Q-fever infection.
mesh_terms: D000034:Abortion, Veterinary / Q000382:microbiology*; D000818:Animals; D002003:Brucella abortus / Q000276:immunology* / Q000302:isolation & purification; D002007:Brucellosis, Bovine / Q000175:diagnosis / Q000276:immunology* / Q000382:microbiology; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis; D003593:Cytoplasm; D003937:Diagnosis, Differential; D005260:Female; D005333:Fetus / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D010920:Placenta / Q000276:immunology; D011247:Pregnancy; D011778:Q Fever / Q000175:diagnosis / Q000662:veterinary; D013194:Staining and Labeling; D013270:Stomach / Q000382:microbiology
chemical_list: D007106:Immune Sera