Induction of immunological tolerance to soluble histocompatibility-2 antigens of mice.
Immunological tolerance to a soluble, partially purified fraction of histocompatibility (H)-2(a) antigen was induced in B10.D2 strain mice. The fraction used was obtained after chromatography on a Sephadex G-150 column. Tolerance of humoral-antibody formation (cytotoxins and hemagglutinins) was observed in mice injected with soluble antigen since birth, but cell-mediated immunity, as measured by skin graft survival, remained intact. Tolerance was shown to be specific for H-2(a) antigens, since treated mice produced a regular humoral-antibody response to H-2(b) antigens. These findings, along with previous observations of the immunogenic and enhancing properties of our partially purified fraction, suggest that the solubilized antigen contains all the major determinants of H-2(a) antigens expressed on lymphoid-cell surfaces.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000917:Antibody Formation; D000941:Antigens / Q000302:isolation & purification; D002850:Chromatography, Gel; D002859:Chromium Isotopes; D000939:Epitopes; D005260:Female; D006084:Graft Rejection; D006386:Hemagglutination Tests; D006648:Histocompatibility; D007108:Immune Tolerance; D051379:Mice; D008815:Mice, Inbred Strains; D016038:Skin Transplantation; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014184:Transplantation, Homologous
D000906:Antibodies; D000941:Antigens; D002859:Chromium Isotopes; D000939:Epitopes