Lysosomal enzyme cytochemistry in acute leukaemia.
A cytochemical study of the lysosomal enzyme beta-glucuronidase in 60 cases of acute leukaemia has shown a qualitative difference in the cytoplasmic distribution of the enzyme between blast cells of the lymphoid and myeloid cell series. This difference provides a useful additional method for cytochemical classification of cell type and is superior in this respect to the other lysosomal enzymes studied (aryl sulphatase and acid phosphatase). The beta-glucuronidase reaction is recommended in those cases of acute leukaemia in which the periodic acid-Schiff reaction is negative or equivocal.
D000135:Acid Phosphatase / Q000032:analysis; D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D001773:Blood Cells; D002648:Child; D002675:Child, Preschool; D003593:Cytoplasm / Q000201:enzymology; D005966:Glucuronidase / Q000032:analysis*; D006651:Histocytochemistry; D006801:Humans; D007938:Leukemia / Q000201:enzymology*; D007945:Leukemia, Lymphoid / Q000201:enzymology; D015470:Leukemia, Myeloid, Acute / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology*; D008875:Middle Aged; D013194:Staining and Labeling; D013429:Sulfatases / Q000032:analysis
D000135:Acid Phosphatase; D013429:Sulfatases; D005966:Glucuronidase