Induction of immunological tolerance in bone marrow-derived lymphocytes of the IgE antibody class.
Administration of the 2,4-dinitrophenyl (Dnp) determinant conjugated to the "nonimmunogenic" copolymer of D-glutamic acid and D-lysine (D-GL) induces Dnp-specific immunological tolerance which is essentially restricted to bone marrow-derived (B) lymphocytes. Previous studies have demonstrated tolerance induction by Dnp-D-GL in predominantly B lymphocytes of the IgG class. The present studies establish and analyze conditions for tolerance induction in Dnp-specific B lymphocytes of the IgE antibody class by taking advantage of: (i) the relatively easy capacity to induce tolerance with Dnp-D-GL in an animal or cell population previously primed to the Dnp determinant; (ii) previously established parameters for the successful adoptive transfer of Dnp-specific IgE antibody responses; and (iii) the capacity to induce, and maintain a state of Dnp-specific tolerance with Dnp-D-GL in adoptive transfer responses. Using these approaches, we have successfully rendered Dnp-specific B cells of the IgE class profoundly and apparently irreversibly tolerant after exposure of such cells to Dnp-D-GL. These observations represent a starting point of wide potential for solving many therapeutic problems concerned with clinical allergies.
D000818:Animals; D000917:Antibody Formation; D000941:Antigens; D001200:Ascaris; D001402:B-Lymphocytes / Q000276:immunology*; D004140:Dinitrophenols; D005971:Glutamates; D006241:Haptens; D007108:Immune Tolerance; D007112:Immunity, Maternally-Acquired; D007114:Immunization; D007116:Immunization, Passive; D007073:Immunoglobulin E / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D008239:Lysine; D051379:Mice; D008805:Mice, Inbred A; D008815:Mice, Inbred Strains; D010323:Passive Cutaneous Anaphylaxis; D011108:Polymers; D011506:Proteins; D005719:gamma-Globulins
D000941:Antigens; D004140:Dinitrophenols; D005971:Glutamates; D006241:Haptens; D007074:Immunoglobulin G; D011108:Polymers; D011506:Proteins; D005719:gamma-Globulins; D007073:Immunoglobulin E; D008239:Lysine