title: Rapid axoplasmic transport of tyrosine hydroxylase in relation to other cytoplasmic constituents.
abstract: The transport of norepinephrine and two key enzymes involved in its synthesis, tyrosine hydroxylase (EC 1.14.3a) and dopamine beta-hydroxylase (EC 1.14.2.1), has been studied in relation to other axonal constituents in ligated chicken sciatic nerves. Norepinephrine, tyrosine hydroxylase, and dopamine beta-hydroxylase activity all increased proximal to the constriction over a 20-hr period. The rate of transport of norepinephrine, tyrosine hydroxylase, and dopamine beta-hydroxylase were calculated as 2.6, 1.9, and 3.4 mm/hr, respectively. The only enzyme marker to show a similar rate of accumulation was acetylcholinesterase (EC 3.1.1.7), a putative marker for endoplasmic reticulum. The rapid axoplasmic transport of tyrosine hydroxylase from the cell bodies to the terminals of sympathetic neurons may be adequate to account for the elevated amounts of this enzyme in chronically active axon terminals, without the necessity to invoke peripheral axonal synthesis of the enzyme to explain such elevated amounts.
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001370:Axonal Transport; D002645:Chickens; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism; D008026:Ligation; D008995:Monoamine Oxidase / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism; D009708:Nucleotidases / Q000378:metabolism; D012584:Sciatic Nerve / Q000201:enzymology / Q000378:metabolism; D013997:Time Factors; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
chemical_list: D014446:Tyrosine 3-Monooxygenase; D004299:Dopamine beta-Hydroxylase; D008995:Monoamine Oxidase; D000110:Acetylcholinesterase; D009708:Nucleotidases; D005966:Glucuronidase; D005956:Glucose-6-Phosphate Isomerase; D009638:Norepinephrine