Isolation of temperature-sensitive mutants of Schizosaccharomyces pombe.
Seventy-one mutants of the yeast Schizosaccharomyces pombe that were able to grow on complete medium at 25 C but not at 37 C were isolated. Strains selected for further study showed: (i) single gene mutation and (ii) cell lengthening at the restrictive temperature. Preliminary characterization of 13 mutants is reported. Seven of them have a less pronounced synthesis of deoxyribonucleic acid at the restrictive temperature, and four of them seem to be affected in cell division.
D002455:Cell Division; D002467:Cell Nucleus; D003433:Crosses, Genetic; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D005796:Genes; D005816:Genetic Complementation Test; D005827:Genetics, Microbial; D009153:Mutagens; D009154:Mutation; D009573:Nitrites; D009604:Nitrosoguanidines; D010641:Phenotype; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D013194:Staining and Labeling; D013696:Temperature; D015003:Yeasts / Q000145:classification / Q000166:cytology / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism
D003470:Culture Media; D009153:Mutagens; D009573:Nitrites; D009604:Nitrosoguanidines; D012313:RNA; D004247:DNA