title: Use of cobalt as a mitochondrial vital stain to study cytoplasmic exchange in matings of the basidiomycete Schizophyllum commune.
abstract: Differential labeling of mates, based on the selective uptake of cobalt by mitochondria of one of the partners, has been used to determine visually by means of phase-contrast microscopy whether transfer of mitochondria occurs after hyphal fusion. Compatible and incompatible matings of the tetrapolar basidiomycete, Schizophyllum commune, were studied. Transfer was detectable in common-A, common-AB, and fully compatible matings. It was not detectable in common-B matings
mesh_terms: D001487:Basidiomycota / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D003035:Cobalt / Q000378:metabolism; D003470:Culture Media; D003593:Cytoplasm / Q000378:metabolism; D003595:Cytoplasmic Streaming; D008722:Methods; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D008928:Mitochondria / Q000378:metabolism; D013194:Staining and Labeling
chemical_list: D003470:Culture Media; D003035:Cobalt