Isolation and characterization of a bacteriophage of Arthrobacter globiformis.
A bacteriophage which reproduces on Arthrobacter globiformis ATCC 8010 was isolated from soil. This bacteriophage, designated phiAG8010, propagates either in soft agar or broth cultures of the host. Because of a slow adsorption rate, neither the latent period nor burst size was determined. The mature virion belongs to Bradley's group B and exhibits a hexagonal head measuring 69 nm (length) by 60 nm (width) attached to a sheathless tail 120 nm long. The buoyant density of the mature virion is 1.534 g/cm(3). The mature virion contains double-stranded DNA with a buoyant density of 1.722 g/cm(3) (equivalent to 63.3% G + C). Of 14 strains (representing 13 species) of Arthrobacter examined, including A. globiformis ATCC 4336, only A. globiformis ATCC 8010 supported replication of phiAG8010.
D001173:Arthrobacter; D001435:Bacteriophages / Q000032:analysis / Q000302:isolation & purification*; D002499:Centrifugation, Density Gradient; D002586:Cesium; D004279:DNA, Viral / Q000302:isolation & purification; D003956:Dialysis; D007700:Kinetics; D008854:Microscopy, Electron; D012988:Soil Microbiology; D013194:Staining and Labeling; D010948:Viral Plaque Assay
D004279:DNA, Viral; D002586:Cesium