Further evidence for oncornaviruses in human milk: the production of cores.
Cores, or nucleoids, have been isolated from particles in human milk. The cores have a density of 1.26-1.27 g/ml and contain a 60-70S RNA in association with an RNA-instructed DNA polymerase. The data offer further evidence of similarity between human milk particles and animal RNA tumor viruses. In addition, core isolation provides a new method for detection of these particles by minimizing the difficulties generated by the presence of cell-associated debris often found in the density region (1.16-1.19 g/ml) characteristic of the intact virion. The procedures described now make available preparations of purified subviral components of a putative human RNA tumor virus.
D000818:Animals; D002499:Centrifugation, Density Gradient; D005260:Female; D006801:Humans; D008324:Mammary Tumor Virus, Mouse / Q000032:analysis / Q000302:isolation & purification; D008722:Methods; D051379:Mice; D008892:Milk / Q000382:microbiology; D008895:Milk, Human / Q000382:microbiology*; D008970:Molecular Weight; D009858:Oncogenic Viruses / Q000302:isolation & purification*; D010740:Phospholipases; D012328:RNA Viruses / Q000032:analysis / Q000201:enzymology / Q000302:isolation & purification*; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D012194:RNA-Directed DNA Polymerase / Q000032:analysis / Q000302:isolation & purification; D013942:Thymine Nucleotides / Q000378:metabolism; D014316:Tritium
D012367:RNA, Viral; D013942:Thymine Nucleotides; D014316:Tritium; D012194:RNA-Directed DNA Polymerase; D010740:Phospholipases