Purification of large amounts of murine ribonucleic acid tumor viruses produced in roller bottle cultures.
Murine ribonucleic acid tumor viruses and C-type virus particles are produced in relatively large quantities in roller bottle cultures. The viruses present in large volumes of culture fluids can be purified by a simple two-step procedure involving polyethylene glycol precipitation and equilibrium centrifugation in sucrose density gradients.
D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D006224:Cricetinae; D046508:Culture Techniques; D005347:Fibroblasts; D017988:Gammaretrovirus / Q000254:growth & development / Q000302:isolation & purification*; D007181:Inclusion Bodies, Viral; D007668:Kidney; D009052:Leukemia Virus, Murine / Q000302:isolation & purification; D008854:Microscopy, Electron; D009858:Oncogenic Viruses / Q000302:isolation & purification*; D012328:RNA Viruses / Q000302:isolation & purification*; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D051381:Rats / Q000196:embryology; D011925:Rauscher Virus / Q000032:analysis / Q000201:enzymology / Q000302:isolation & purification; D012190:Retroviridae / Q000254:growth & development / Q000302:isolation & purification; D014776:Virus Cultivation
D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase