Membrane molecules determined by the H-2 associated immune response region: isolation and some properties.
Membrane associated molecules that are probably glycoproteins could be specifically precipitated from NP-40 detergent solubilized extracts of radiolabeled mouse spleen or lymph node cells by antisera produced in congenic strain combinations differing only in the Ir gene region which is linked to the H-2 genes. These Ir region products were designated Lna (lymph node antigen) to conform to previous serological work.Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of unreduced specific immune precipitates revealed the presence of a possible dimer form, while reduced samples showed only a single peak equivalent to 30,000 daltons. Thus the Lna molecules are clearly distinct from the H-2D and H-2K molecules, which are about 45,000 daltons. Anti-Lna antibodies of different specificity can be present in a single serum; there were at least two separate antigen molecules present in one haplotype tested.
D000818:Animals; D000936:Antigen-Antibody Complex; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000302:isolation & purification*; D001345:Autoradiography; D002462:Cell Membrane / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D008040:Genetic Linkage; D005838:Genotype; D006648:Histocompatibility; D006649:Histocompatibility Antigens; D007106:Immune Sera; D008198:Lymph Nodes / Q000276:immunology; D008214:Lymphocytes; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D011233:Precipitin Tests; D013045:Species Specificity; D013154:Spleen / Q000276:immunology; D014316:Tritium
D000936:Antigen-Antibody Complex; D000941:Antigens; D003902:Detergents; D000939:Epitopes; D006649:Histocompatibility Antigens; D007106:Immune Sera; D014316:Tritium