Specific inhibition of plaque formation to phosphorylcholine by antibody against antibody.
Spleen cells from BALB/c mice immunized with heat-killed rough pneumococci (strain R36A) or spleen cells from normal mice immunized in vitro with the same antigen produce direct hemolytic plaques against sheep erythrocytes coated with pneumococcal C polysaccharide or conjugated with phosphorylcholine. Formation of plaques is specifically inhibited by phosphorylcholine or by antiserum to mouse immunoglobulin A myeloma protein which binds phosphorylcholine. Thus, the myeloma proteins and normal BALB/c antibodies share similar idiotypic determinants. This experimental system is suitable for probing the role of the antigen receptor in the immune response.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000917:Antibody Formation; D002794:Choline; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology; D006462:Hemolytic Plaque Technique; D007114:Immunization; D051379:Mice; D008815:Mice, Inbred Strains; D009194:Myeloma Proteins; D011135:Polysaccharides, Bacterial; D012756:Sheep / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013296:Streptococcus pneumoniae / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D009194:Myeloma Proteins; D011135:Polysaccharides, Bacterial; D002794:Choline