Characteristics and purification of PRR1, an RNA phage specific for the broad host range Pseudomonas R1822 drug resistance plasmid.
A new drug resistance plasmid-dependent RNA containing phage resembling coliphage f2 in its particle size and density is described. The phage, PRR1, will only productively infect some R(+) hosts containing the Pseudomonas drug resistance plasmid R1822. The membrane filter-salt elution patterns, RNase sensitivity, inactivation in low ionic strength solutions, and host range serve to distinguish PRR1 from coliphage f2 and two other Pseudomonas RNA phages, 7s and PP7.
D001435:Bacteriophages / Q000032:analysis / Q000254:growth & development / Q000302:isolation & purification*; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D003090:Coliphages / Q000032:analysis; D004352:Drug Resistance, Microbial; D004755:Enterobacteriaceae; D005111:Extrachromosomal Inheritance; D008851:Micropore Filters; D008854:Microscopy, Electron; D010772:Phosphotungstic Acid; D011549:Pseudomonas / Q000187:drug effects*; D012367:RNA, Viral / Q000032:analysis; D012260:Ribonucleases; D013194:Staining and Labeling; D014764:Viral Proteins
D012367:RNA, Viral; D014764:Viral Proteins; D010772:Phosphotungstic Acid; D012260:Ribonucleases