Separation of lipase-positive cells from suspensions of pancreas cells in an isokinetic gradient of Ficoll in tissue culture medium.
Lipase-positive cells were separated from suspensions of pancreas cells both by velocity sedimentation and by isopycnic sedimentation. Lipase-positive cells were 51.0 +/- 9.6% of the disaggregated pancreas cells in the starting sample suspension. The purest gradient fractions from experiments using velocity sedimentation for cell separation contained a mean of 98.8 +/- 0.6% lipase-positive cells. Cell separation using isopycnic centrifugation was less effective than cell separation using velocity sedimentation and resulted in a mean purity of only 89.8 +/- 6.8% lipase-positive cells. Lipase positivity was assessed using the Gomori technic with the conditions for fixation slightly modified to be suitable for disaggregated cells.
D000818:Animals; D002467:Cell Nucleus; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D003470:Culture Media; D046508:Culture Techniques; D003584:Cytological Techniques; D004912:Erythrocytes; D008049:Lipase; D008297:Male; D008407:Mast Cells; D010179:Pancreas / Q000166:cytology* / Q000201:enzymology; D013194:Staining and Labeling; D013535:Suspensions
D003470:Culture Media; D013535:Suspensions; D008049:Lipase