Isolation of temperature-sensitive mutants of murine sarcoma virus.
Three separate murine sarcoma virus nonproducer cell lines have been isolated which are temperature sensitive for the maintenance of transformation. In each case, a viral rather than a cellular genetic mutation is the reason for the temperature-sensitive effect. Superinfection of one of the mutants with murine leukemia virus overcomes the temperature-sensitive change in the transformed state.
D000818:Animals; D001374:Azacitidine; D001973:Bromodeoxyuridine; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells; D003260:Contact Inhibition; D017988:Gammaretrovirus / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D006378:Helper Viruses / Q000254:growth & development; D007668:Kidney; D009052:Leukemia Virus, Murine / Q000254:growth & development; D051379:Mice; D009153:Mutagens; D009154:Mutation; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000096:biosynthesis; D051381:Rats; D012513:Sarcoma, Experimental / Q000382:microbiology; D013696:Temperature; D014316:Tritium; D010948:Viral Plaque Assay; D014779:Virus Replication
D009153:Mutagens; D012367:RNA, Viral; D014316:Tritium; D001973:Bromodeoxyuridine; D001374:Azacitidine