On the process of cellular division in Escherichia coli. VI. Use of a methocel-autoradiographic method for the study of cellular division in Escherichia coli.
A technique allowing chain formation by Escherichia coli for the analysis of the segregation of certain cellular components by autoradiographic methods has been developed. Deoxyribonucleic acid labeled with (3)H-thymidine does not appear to segregate preferentially with one or another of the bacteria. Membrane lipids labeled with either (3)H-oleate or (3)H-glycerol are evenly distributed among all progeny cells. A similar pattern of distribution of cell wall material is observed when (3)H-diaminopimelate is used as the marker.
D001345:Autoradiography; D002455:Cell Division; D002462:Cell Membrane / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D002725:Chloroform; D002876:Chromosomes, Bacterial; D003470:Culture Media; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis; D004926:Escherichia coli / Q000032:analysis / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D005827:Genetics, Microbial; D005990:Glycerol / Q000378:metabolism; D008055:Lipids / Q000032:analysis; D000432:Methanol; D008722:Methods; D008751:Methylene Blue; D008858:Microscopy, Phase-Contrast; D008961:Models, Structural; D009829:Oleic Acids / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis; D010867:Pimelic Acids / Q000378:metabolism; D013194:Staining and Labeling; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D003470:Culture Media; D004269:DNA, Bacterial; D008055:Lipids; D009829:Oleic Acids; D010743:Phospholipids; D010867:Pimelic Acids; D014316:Tritium; D002725:Chloroform; D005990:Glycerol; D008751:Methylene Blue; D013936:Thymidine; D000432:Methanol