Use of peroxidase-conjugated antiglobulin as an alternative to immunofluorescence for the detection of antinuclear factor in serum.
A method of detecting serum antinuclear factor by the use of antiglobulin conjugated with horseradish peroxidase has been compared with a standard procedure using a fluorescent label. The horseradish peroxidase conjugate was evaluated by block titration against positive serum and by its performance as a test reagent for screening and titrating sera. It is concluded that the horse-radish peroxidase method, in which an ordinary light microscope is employed, provides a satisfactory alternative to immunofluorescence for routine tests for antinuclear factor.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000974:Antibodies, Antinuclear / Q000032:analysis*; D002623:Chemistry Techniques, Analytical; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D006801:Humans; D008722:Methods; D008856:Microscopy, Fluorescence; D010544:Peroxidases; D011817:Rabbits; D013194:Staining and Labeling
D000888:Antibodies, Anti-Idiotypic; D000974:Antibodies, Antinuclear; D010544:Peroxidases