A bacteriophage of Bacillus subtilis which forms plaques only at temperatures above 50 C. I. Physical and chemical characteristics of TSP-1.
Bacteriophage TSP-1 was isolated from soil in a search for phage which would form plaques on Bacillus subtilis W168 at 53 C. It forms clear plaques only at temperatures from 50 to 55 C. Approximately 95% of the free phage adsorb after 2 min at 53 C. The lytic cycle is between 55 and 60 min long with a burst size of about 55 particles per infected bacterium. The phage was shown to contain double-stranded deoxyribonucleic acid with a base composition of 44.7% guanine plus cytosine. This deoxyribonucleic acid does not contain a base analogue for thymine and has a molecular weight estimated at 56 x 10(6) daltons.
D000327:Adsorption; D001412:Bacillus subtilis; D001433:Bacteriolysis; D001435:Bacteriophages / Q000032:analysis / Q000254:growth & development* / Q000302:isolation & purification; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D002854:Chromatography, Paper; D003596:Cytosine / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D006147:Guanine / Q000032:analysis; D006358:Hot Temperature; D008854:Microscopy, Electron; D008970:Molecular Weight; D010759:Phosphorus Isotopes; D012988:Soil Microbiology; D013053:Spectrophotometry; D013194:Staining and Labeling; D013997:Time Factors; D014764:Viral Proteins; D014776:Virus Cultivation; D014779:Virus Replication
D002712:Chlorides; D004279:DNA, Viral; D010759:Phosphorus Isotopes; D014764:Viral Proteins; D002586:Cesium; D006147:Guanine; D003596:Cytosine