Evans blue as counterstain in the demonstration of muscle antibodies by immunofluorescence in myasthenia gravis.
The binding of muscle antibodies to skeletal muscle was studied by immunofluorescence techniques. The non-specific fluorescence was not significantly reduced by absorption of the conjugate by tissue powder but was abolished by counterstaining with a 0.1% Evans blue. Possible explanations for this effect are discussed. By this method, immunofluorescence may become more useful in the demonstration of muscle antibodies.
D000818:Animals; D001666:Binding Sites, Antibody; D004396:Coloring Agents; D005455:Fluorescent Antibody Technique; D006386:Hemagglutination Tests; D006801:Humans; D007074:Immunoglobulin G; D008722:Methods; D008856:Microscopy, Fluorescence; D009132:Muscles / Q000276:immunology*; D009157:Myasthenia Gravis / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D013194:Staining and Labeling
D004396:Coloring Agents; D007074:Immunoglobulin G