title: Binding of aggregated gamma-globulin to activated T lymphocytes in the guinea pig.
abstract: Heat-aggregated guinea pig gamma-globulin was shown to bind to the surface membrane of a subclass of guinea pig T lymphocytes. Cells of this subpopulation were identified as T lymphocytes because these cells did not stain for surface Ig (a B-cell marker) but did form spontaneous E-rosettes with rabbit erythrocytes (a T-cell marker). A strikingly high proportion of such aggregate-binding (Agg(+)), E-rosette-forming (E-rosette(+)), but surface Ig-negative (Ig(-)) cells were found in an inflammatory exudate. Thus purified peritoneal exudate lymphocytes (PELs) are known to consist of over 90% T cells, and 59% of these cells bound aggregates. 10% of these Agg+ Ig- E-rosette+ cells were found in draining lymph node cell populations and none in thymus cell populations. The high frequency amongst PELs suggested that these Aggregate+ Ig- E-rosette+ cells might be activated T cells as these are known to occur in high proportion in PEL populations. Confirmatory evidence for this postulate was provided by the striking increase (from 10% to 46%) of Ig- E-rosette+ cells that bound aggregates when lymph node cells were activated by antigen stimulation in vitro.
mesh_terms: D000818:Animals; D000941:Antigens; D001666:Binding Sites, Antibody; D002462:Cell Membrane / Q000276:immunology*; D002478:Cells, Cultured; D004912:Erythrocytes / Q000276:immunology; D006168:Guinea Pigs; D006358:Hot Temperature; D007104:Immune Adherence Reaction; D007141:Immunoglobulin Fc Fragments; D008213:Lymphocyte Activation; D008297:Male; D011817:Rabbits / Q000276:immunology; D012756:Sheep / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D005719:gamma-Globulins
chemical_list: D000941:Antigens; D007141:Immunoglobulin Fc Fragments; D005719:gamma-Globulins