Agar diffusion method for negative staining of microbial suspensions in salt solutions.
Microbial suspensions in a saline medium can be prepared for negative staining by a simple agar diffusion method that achieves a high degree of particle concentration.
D000256:Adenoviridae; D000362:Agar; D001419:Bacteria / Q000166:cytology; D003470:Culture Media; D004058:Diffusion; D004926:Escherichia coli / Q000166:cytology; D006652:Histological Techniques; D008854:Microscopy, Electron; D008982:Molybdenum; D010772:Phosphotungstic Acid; D000644:Quaternary Ammonium Compounds; D012087:Reoviridae; D018108:Respirovirus; D012996:Solutions; D013194:Staining and Labeling; D014780:Viruses
D003470:Culture Media; D000644:Quaternary Ammonium Compounds; D012996:Solutions; D010772:Phosphotungstic Acid; D008982:Molybdenum; D000362:Agar