Genetics of restricted antibodies to streptococcal group polysaccharides in mice. I. Strain differences of isoelectric focusing spectra of group A hyperimmune antisera.
The immune response of nine inbred and one outbred strain of mice to the streptococcal group A polysaccharide was investigated with respect to magnitude and restriction. Analytical isoelectric focusing served as a tool to estimate the degree of restriction of Group A polysaccharide-specific antibodies. It proved feasible to distinguish low and intermediate from high responder strains, and to delineate strain-specificity of isoelectric focusing spectra of the immune sera. For example, immune sera of BALB/c mice, restricted high responders, and of C57BL/6 mice, heterogeneous low responders, had distinct focusing properties. Responsiveness was a dominant autosomal genetic trait in C57BL/6 x BALB/c F(1) hybrid mice, irrespective of the maternal and the paternal genotype; the immune sera of these mice had their own, rather uniform isoelectric focusing spectra whereby structural genes of the low responder strain were expressed to predominant levels in 81% of the hybrids. Responsiveness in C57BL/6 x BALB/c F(2) progeny segregated into 79% high and 21% low responders, and showed no genetic linkage to the following characteristics: hair color, sex, H-2 type, and Ig allotype of the heavy chain. The isoelectric focusing properties of these immune sera indicated segregation into patterns like BALB/c mice (40%), F(1) hybrids (48%), and C57BL/6 mice (12%). Since this segregation is independent of any of the above criteria in these F(2) mice a regulatory gene(s) is postulated that controls the clonal pattern of the immune response.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial; D001345:Autoradiography; D001428:Bacterial Vaccines; D003488:Cyanogen Bromide; D003601:Cytotoxicity Tests, Immunologic; D004586:Electrophoresis; D006650:Histocompatibility Testing; D007106:Immune Sera; D005779:Immunodiffusion; D007125:Immunogenetics; D007457:Iodine Radioisotopes; D007519:Isoantigens / Q000032:analysis; D007525:Isoelectric Focusing; D008198:Lymph Nodes / Q000166:cytology; D051379:Mice; D008815:Mice, Inbred Strains; D011135:Polysaccharides, Bacterial; D011233:Precipitin Tests; D013045:Species Specificity; D013194:Staining and Labeling; D013291:Streptococcus / Q000276:immunology*; D014439:Tyramine
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D007106:Immune Sera; D007457:Iodine Radioisotopes; D007519:Isoantigens; D011135:Polysaccharides, Bacterial; D003488:Cyanogen Bromide; D014439:Tyramine