Coupling of peroxidase to poliovirus antibody: characteristics of the conjugates and their use in virus detection.
Antibody to poliovirus type 1 (Po-1) was coupled to peroxidase by use of glutaraldehyde or 4, 4' -difluoro,3, 3' -dinitro diphenyl sulfone. Glutaraldehyde was found to be the superior coupling agent, yielding conjugates that had up to 2.8 x 10(4) enzyme units/ml (75% of total enzyme input). Conjugates migrated as a single band when centrifuged in sucrose density gradients, demonstrating that the purification procedure used was effective in removing both noncoupled enzyme and heterogeneous antibody components. Conjugates were specific for Po-1 and did not adsorb to cells infected with unrelated enterovirus types. Adsorption of conjugates to Po-1-infected cells was demonstrable within 6 h postinfection.
D000914:Antibodies, Viral; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D004770:Enterovirus / Q000276:immunology; D029822:Enterovirus B, Human / Q000276:immunology; D005976:Glutaral; D006861:Hydrogen Peroxide / Q000378:metabolism; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D008722:Methods; D010544:Peroxidases / Q000032:analysis*; D017955:Poliovirus / Q000276:immunology*; D013194:Staining and Labeling; D013501:Surface-Active Agents; D010948:Viral Plaque Assay
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D013501:Surface-Active Agents; D006861:Hydrogen Peroxide; D010544:Peroxidases; D005976:Glutaral