Barbiturates: radioimmunoassay.
The development of a radioimmunoassay for barbiturate is described. The barbiturate is made antigenic by coupling it to a protein, bovine gamma globulin. The radioimmunoassay can measure as little as 5 nanograms of barbiturate.
D000818:Animals; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000138:chemical synthesis; D001463:Barbiturates / Q000032:analysis*; D001665:Binding Sites; D002247:Carbon Isotopes; D002417:Cattle; D007106:Immune Sera; D007114:Immunization; D008722:Methods; D011817:Rabbits; D011863:Radioimmunoassay; D005719:gamma-Globulins
D000941:Antigens; D001463:Barbiturates; D002247:Carbon Isotopes; D007106:Immune Sera; D005719:gamma-Globulins