The molecular basis of codominant expression of the histocompatibility-2 genetic region.
H-2 alloantigens in an intact, undegraded form can be solubilized by the nonionic detergent NP-40 and isolated by indirect immunoprecipitation and electrophoresis in sodium dodecyl sulfate on polyacrylamide gels. With the use of an immunological precipitation method, the question about the number of cellular H-2 gene products in heterozygous cells has been partially resolved. At least four separable gene products were detected in cells heterozygous at the H-2 genetic region-one for each of the H-2D genes of the two parental haplotypes, and one for each of the H-2K genes of the two parental haplotypes. This is in contrast to the two H-2 gene products reported previously for cells homozygous at the H-2 region. The findings establish that the two parental H-2 haplotypes on homologous chromosomes in heterozygous cells are ultimately expressed as different glycoprotein molecules.
D000178:Acrylamides; D000818:Animals; D000918:Antibody Specificity; D000941:Antigens; D002462:Cell Membrane / Q000276:immunology*; D000939:Epitopes; D005643:Fucose; D005799:Genes, Dominant; D006023:Glycoproteins; D006168:Guinea Pigs; D006238:Haploidy; D006579:Heterozygote; D006648:Histocompatibility; D006720:Homozygote; D007106:Immune Sera; D007122:Immunoelectrophoresis; D051379:Mice; D008815:Mice, Inbred Strains; D011233:Precipitin Tests; D012967:Sodium Dodecyl Sulfate; D012995:Solubility; D013154:Spleen / Q000276:immunology; D013501:Surface-Active Agents; D014316:Tritium
D000178:Acrylamides; D000941:Antigens; D000939:Epitopes; D006023:Glycoproteins; D007106:Immune Sera; D013501:Surface-Active Agents; D014316:Tritium; D005643:Fucose; D012967:Sodium Dodecyl Sulfate