Group B arbovirus structural and nonstructural antigens. I. Serological identification of Saint Louis encephalitis virus soluble antigens.
Saint Louis encephalitis virus-infected cells were solubilized with Brij-58 nonionic detergent, and the clarified supernatant fluid was analyzed by diethyl-aminoethyl-cellulose chromatography. Three serologically distinguishable antigens were identified in viral protein peaks which eluted from diethylaminoethyl-cellulose at 0.05, 0.075, 0.125 and 0.2 m KCl. Antigen I was found in all column peaks, whereas antigen II was found only in the 0.05 m KCl eluate and antigen III was found only in the 0.2 m eluate. Immunodiffusion studies revealed that antigens I and II share at least one antigenic determinant and that antigen III is immunologically distinct from either antigen I or II. Polyacrylamide gel electrophoresis of (3)H-leucine-labeled concentrated column peaks revealed that antigens I and II contain the major viral structural envelope and core proteins, respectively. Antigen III contains the largest virus-induced nonstructural polypeptide observed by polyacrylamide gel electrophoresis of (3)H-leucine-labeled Saint Louis encephalitis virus-infected cells.
D000818:Animals; D000956:Antigens, Viral; D001202:Ascitic Fluid / Q000276:immunology; D002848:Chromatography, DEAE-Cellulose; D003168:Complement Fixation Tests; D046508:Culture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D004665:Encephalitis Virus, St. Louis / Q000276:immunology; D004668:Encephalitis Viruses / Q000276:immunology*; D000939:Epitopes; D005944:Glucosamine / Q000378:metabolism; D005779:Immunodiffusion; D007668:Kidney; D007930:Leucine / Q000378:metabolism; D051379:Mice; D012995:Solubility; D013552:Swine; D014316:Tritium; D014764:Viral Proteins / Q000032:analysis; D014776:Virus Cultivation
D000956:Antigens, Viral; D000939:Epitopes; D014764:Viral Proteins; D014316:Tritium; D007930:Leucine; D005944:Glucosamine