Isolation and preliminary characterization of bacteriophages for Bdellovibrio bacteriovorus.
Ten bacteriophages that attack and lyse saprophytic strains of Bdellovibrio bacteriovorus were isolated. Morphological, serological, and host-range studies revealed that there were four different bdellovibrio phages present among the isolates. One of the phages lysed a strain of B. bacteriovorus that requires the presence of a suitable bacterial host for growth. The phage attached to the bdellovibrio cells in the absence of the bacterial host cells; lysis occurred only in the presence of host cells. The 19 saprophytic bdellovibrio strains employed in the phage host-range studies were grouped on the basis of their susceptibility to phage lysis.
D000956:Antigens, Viral; D001419:Bacteria / Q000145:classification / Q000254:growth & development; D001435:Bacteriophages / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification; D002499:Centrifugation, Density Gradient; D003429:Cross Reactions; D004926:Escherichia coli; D008242:Lysogeny; D008854:Microscopy, Electron; D009500:Neutralization Tests; D013194:Staining and Labeling; D013395:Sucrose; D010948:Viral Plaque Assay; D014764:Viral Proteins
D000956:Antigens, Viral; D014764:Viral Proteins; D013395:Sucrose