Studies on purified eel acetylcholine receptor and anti-acetylcholine receptor antibody.
Rabbit antiserum against purified Electrophorus electricus acetylcholine receptor is studied using an immunoprecipitin assay to measure either antibody titer or concentration of toxin-binding sites in solubilized receptor preparations. This antiserum, unlike control serum, blocks the electrophysiological response of the electroplax to carbamylcholine. Toxin (alpha-neurotoxin, Naja naja) and several cholinergic ligands produce partial inhibition of the reaction of antiserum with purified acetylcholine receptor. Evidence is presented that some of the toxin-binding sites on receptor, purified by affinity chromatography on toxin-agarose conjugates, are occluded by toxin. In addition, evidence is presented that antireceptor antiserum will cause precipitation of more toxin-binding sites present in an initial extract than in purified receptor preparations.
D000085:Acetates; D000818:Animals; D000936:Antigen-Antibody Complex; D000937:Antigen-Antibody Reactions / Q000187:drug effects; D001665:Binding Sites; D001666:Binding Sites, Antibody; D002794:Choline / Q000494:pharmacology; D002846:Chromatography, Affinity; D004593:Electrophorus; D000939:Epitopes; D007457:Iodine Radioisotopes; D011233:Precipitin Tests; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D011809:Quinones / Q000494:pharmacology; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D011950:Receptors, Cholinergic; D011955:Receptors, Drug; D012911:Snakes; D014316:Tritium; D014403:Tubocurarine / Q000494:pharmacology; D014688:Venoms
D000085:Acetates; D000936:Antigen-Antibody Complex; D000939:Epitopes; D007457:Iodine Radioisotopes; D000644:Quaternary Ammonium Compounds; D011809:Quinones; D011950:Receptors, Cholinergic; D011955:Receptors, Drug; D014688:Venoms; D014316:Tritium; D002794:Choline; D014403:Tubocurarine