Electron microscope methods for the identification of adenoviruses isolated in micro tissue cultures.
A tissue culture micromethod is described for adenovirus isolation and preparation for presumptive identification by electron microscopy. These procedures are easier, more economical, and faster than conventional methods. The micro techniques make it more feasible to utilize direct visualization of virus in infected cells as an adjunctive diagnostic and research tool.
D000085:Acetates; D000256:Adenoviridae / Q000145:classification / Q000302:isolation & purification* / Q000472:pathogenicity; D002245:Carbon Dioxide; D002951:Citrates; D046508:Culture Techniques; D003588:Cytopathogenic Effect, Viral; D005069:Evaluation Studies as Topic; D006367:HeLa Cells / Q000382:microbiology; D006801:Humans; D007181:Inclusion Bodies, Viral; D007854:Lead; D008722:Methods; D008854:Microscopy, Electron; D013194:Staining and Labeling; D013997:Time Factors; D014501:Uranium; D014776:Virus Cultivation
D000085:Acetates; D002951:Citrates; D002245:Carbon Dioxide; D007854:Lead; D014501:Uranium