Use of tolylene diisocyanate for the preparation of a peroxidase-labelled antibody conjugate. Quantitation of the amount of diisocyanate bound.
D000818:Animals; D001059:Apoproteins; D001666:Binding Sites, Antibody; D002417:Cattle; D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D003485:Cyanates; D006041:Goats / Q000276:immunology; D006801:Humans; D006868:Hydrolysis; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G; D008970:Molecular Weight; D010544:Peroxidases; D010944:Plants / Q000201:enzymology; D011485:Protein Binding; D011817:Rabbits; D012710:Serum Albumin, Bovine; D013053:Spectrophotometry; D013696:Temperature; D014050:Toluene; D014052:Toluidines; D005719:gamma-Globulins
D001059:Apoproteins; D003485:Cyanates; D007074:Immunoglobulin G; D014052:Toluidines; D005719:gamma-Globulins; D012710:Serum Albumin, Bovine; D014050:Toluene; D010544:Peroxidases