Evaluation of new kits for the assessment in vitro of thyroid function by determination of serum total thyroxine, free TBG capacity, and free thyroxine index Sephadex G-25 and 125I-labelled triiodothyronine and thyroxine.
A trial was carried out on 134 patients of new kits (Ames Co) using columns of Sephadex G-25 for the determination of serum total thyroxine (Tetralute test) and for the indirect estimation of serum free thyroxine-binding globulin capacity (Trilute test). Both new methods were quicker and easier than the reference resin methods and of similar precision. The two measurements when combined to give a free thyroxine index (Trilute-Tetralute-FTI) increased further the diagnostic discrimination and usefulness of the tests. The method for the determination of serum thyroxine can be modified to give a direct estimate of serum free thyroxine, expressed as a free thyroxine index. This new single-column technique, called the ;single-column free thyroxine index', gave a good correlation with clinical thyroid status in a preliminary trial of 45 patients.
D000293:Adolescent; D000328:Adult; D000368:Aged; D003276:Contraceptives, Oral; D005260:Female; D006801:Humans; D006980:Hyperthyroidism / Q000097:blood; D007037:Hypothyroidism / Q000097:blood; D007457:Iodine Radioisotopes; D008297:Male; D008722:Methods; D008875:Middle Aged; D012712:Serum Globulins; D013960:Thyroid Function Tests / Q000295:instrumentation*; D013974:Thyroxine / Q000097:blood*; D013975:Thyroxine-Binding Proteins / Q000097:blood*; D014284:Triiodothyronine
D003276:Contraceptives, Oral; D007457:Iodine Radioisotopes; D012712:Serum Globulins; D013975:Thyroxine-Binding Proteins; D014284:Triiodothyronine; D013974:Thyroxine