title: Molecular relatedness of mammalian RNA tumor viruses as determined by DNA hybridization.
abstract: Each of six mammalian C-type viruses-including two feline leukemia viruses, three murine leukemia viruses, and the human "candidate" virus RD-114-can be distinguished from each other by hybridizing DNA synthesized by viral reverse transcriptase with viral RNA. Characterization of the DNA . RNA hybrids by hydroxylapatite chromatography revealed nucleotide sequence diversity among the viruses, detectable both by the amount of cross-hybridization and by the decreased thermal stability of heterologous hybrids.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002415:Cats; D004279:DNA, Viral / Q000096:biosynthesis / Q000378:metabolism; D006801:Humans; D005256:Leukemia Virus, Feline / Q000032:analysis; D009052:Leukemia Virus, Murine / Q000032:analysis; D051379:Mice; D008979:Moloney murine leukemia virus / Q000032:analysis; D009693:Nucleic Acid Hybridization; D009858:Oncogenic Viruses / Q000032:analysis* / Q000145:classification; D011119:Polynucleotides / Q000378:metabolism; D012328:RNA Viruses / Q000032:analysis* / Q000145:classification; D012367:RNA, Viral / Q000032:analysis* / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D011925:Rauscher Virus / Q000032:analysis; D012190:Retroviridae / Q000032:analysis / Q000145:classification; D013045:Species Specificity
chemical_list: D004279:DNA, Viral; D011119:Polynucleotides; D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase