Studies on the translocation of noradrenaline-containing vesicles in post-ganglionic sympathetic neurones in vitro. Inhibition of movement by colchicine and vinblastine and evidence for the involvement of axonal microtubules.
1. Methods are presented for studying axonal transport mechanisms in preparations of constricted hypogastric nerve/inferior mesenteric ganglia maintained in vitro for periods up to 48 hr. Under these conditions the ultrastructure of the tissue is excellently preserved.2. The proximo-distal movement of noradrenaline and noradrenaline storage vesicles in the non-myelinated axons of these preparations is inhibited by both colchicine (10 mug/ml.) and vinblastine sulphate (1 mug/ml.) whilst the movement of mitochondria appears to be unaffected.3. Neither colchicine nor vinblastine sulphate depletes accumulated dense-cored vesicles of their stores of noradrenaline.4. These drugs reduce the accumulation of noradrenaline and densecored vesicles against a constriction when they are in contact with the nerve trunks only, and are denied direct access to the nerve cell bodies in the ganglion.5. The only other morphological change that can be attributed to the action of colchicine and vinblastine is a marked reduction in the number of axonal microtubules.6. The experiments provide strong support for the view that the axonal system of microtubules is closely involved in the proximo-distal movement of noradrenaline storage vesicles within noradrenergic neurones. The microtubular system does not appear to be involved in mitochondrial movement.
D000818:Animals; D001338:Autonomic Fibers, Postganglionic / Q000187:drug effects*; D001370:Axonal Transport / Q000187:drug effects; D001369:Axons / Q000187:drug effects*; D002415:Cats; D003078:Colchicine / Q000494:pharmacology*; D003593:Cytoplasm; D003594:Cytoplasmic Granules / Q000187:drug effects*; D005260:Female; D002479:Inclusion Bodies / Q000187:drug effects; D008026:Ligation; D008297:Male; D008854:Microscopy, Electron; D008870:Microtubules / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects; D008933:Mitochondrial Swelling; D009068:Movement / Q000187:drug effects; D009638:Norepinephrine; D012583:Schwann Cells / Q000166:cytology; D014747:Vinblastine / Q000494:pharmacology*
D014747:Vinblastine; D003078:Colchicine; D009638:Norepinephrine