Concentration and purification of hepatitis B antigen with polyethylene glycol and polyelectrolyte 60, a cross-linked copolymer of isobutylene maleic anhydride.
A simple two-step procedure is described for the concentration and purification of hepatitis B antigen. The antigen is precipitated by polyethylene glycol and then adsorbed on and eluted from an insoluble polyelectrolyte, polyelectrolyte 60, which is a cross-linked copolymer of isobutylene maleic anhydride.
D000327:Adsorption; D000418:Albumins / Q000032:analysis; D000475:Alkenes; D000812:Anhydrides; D001797:Blood Protein Electrophoresis; D001798:Blood Proteins / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D006018:Glycols; D006511:Hepatitis B Antigens / Q000302:isolation & purification*; D006801:Humans; D007122:Immunoelectrophoresis; D008298:Maleates; D011095:Polyethylenes; D011108:Polymers; D005719:gamma-Globulins / Q000032:analysis
D000418:Albumins; D000475:Alkenes; D000812:Anhydrides; D001798:Blood Proteins; D006018:Glycols; D006511:Hepatitis B Antigens; D008298:Maleates; D011095:Polyethylenes; D011108:Polymers; D005719:gamma-Globulins; C002189:polyelectrolyte 60