title: Detection of avian tumor virus RNA in uninfected chicken embryo cells.
abstract: Uninfected chicken embryo cells were analyzed for the presence of viral ribonucleic acid (RNA) by molecular hybridization with the single-stranded deoxyribonucleic acid (DNA) product of the RNA-dependent DNA polymerase contained in avian sarcoma-leukosis virions. Viral RNA was detected in all cells which contained the avian tumor virus group-specific antigen and the virus-related helper factor. The amounts of viral RNA in these cells ranged from approximately 3 to 40 copies of viral-specific sequences per cell. In general, the viral RNA content correlated with the level of helper activity in the cells. Cells infected with Rous-associated virus 2 contained 3,000 to 4,000 copies of viral RNA per cell. RNA from these infected cells hybridized with nearly 100% of the viral (3)H-DNA. By contrast, a maximum of less than 50% hybridization was obtained with RNA from the uninfected helper-positive cells, suggesting that not all of the viral RNA sequences were present in these cells. No viral RNA was detected in cells which lacked group-specific antigen and helper activity. Under the conditions used in these studies, less than 0.3 viral genome equivalents of RNA per cell would have been detected.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification; D001230:Aspergillus / Q000201:enzymology; D001354:Avian Leukosis Virus / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification; D001358:Avian Sarcoma Viruses / Q000254:growth & development / Q000302:isolation & purification; D002478:Cells, Cultured / Q000276:immunology / Q000382:microbiology; D002642:Chick Embryo; D004277:DNA, Single-Stranded; D004279:DNA, Viral / Q000096:biosynthesis; D003851:Deoxyribonucleases; D006378:Helper Viruses; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000302:isolation & purification*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012526:Satellite Viruses / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate; D014316:Tritium
chemical_list: D000956:Antigens, Viral; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D012367:RNA, Viral; D014316:Tritium; D012967:Sodium Dodecyl Sulfate; D012194:RNA-Directed DNA Polymerase; D003851:Deoxyribonucleases