Radioimmunoassay for human alpha 1 -fetoprotein.
A method of radioimmunoassay has been developed for the quantitation of alpha(1)-fetoprotein in human serum. The assay requires 20 mul of serum, can be completed in 8 hr, and can reproducibly detect concentrations of 20 ng of alpha(1)-fetoprotein per ml of serum. Hence, the method is about 500-fold more sensitive for the detection of alpha(1)-fetoprotein than the Ouchterlony teohnique currently in general use. The procedure is of potential clinical value as an aid in the diagnosis of cancer and a number of noncancerous hepatic diseases.
D000328:Adult; D000510:Alpha-Globulins / Q000032:analysis*; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000097:blood; D005326:Fetal Proteins / Q000032:analysis*; D006041:Goats / Q000276:immunology; D006506:Hepatitis A / Q000097:blood; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007456:Iodine Isotopes; D008107:Liver Diseases / Q000097:blood; D008113:Liver Neoplasms; D008297:Male; D009373:Neoplasms, Germ Cell and Embryonal / Q000097:blood; D011233:Precipitin Tests; D011863:Radioimmunoassay; D013736:Testicular Neoplasms / Q000097:blood
D000510:Alpha-Globulins; D005326:Fetal Proteins; D007456:Iodine Isotopes