Morphology and physical properties of Staphylococcus bacteriophage P11-M15.
The Group B Staphylococcus phage P11-M15 is shown to be 51% protein and 49% deoxyribonucleic acid (DNA). The intact virion has a molecular weight of 66.7 x 10(6) daltons. The purified viral DNA has a molecular weight of 32.7 x 10(6) daltons. The intact virion is shown to be composed of a polyhedral head which is attached at one of its vertices to a flexible tail having helical symmetry. The tail structure is terminated by a complex baseplate which has sixfold symmetry. The virion contains a single molecule of double-stranded DNA which has no apparent single-strand nicks or single-stranded terminal redundancies.
D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D002852:Chromatography, Ion Exchange; D004279:DNA, Viral / Q000032:analysis*; D005613:Freeze Etching; D008854:Microscopy, Electron; D008961:Models, Structural; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D010772:Phosphotungstic Acid; D013194:Staining and Labeling; D013210:Staphylococcus; D013204:Staphylococcus Phages / Q000032:analysis* / Q000302:isolation & purification; D014764:Viral Proteins / Q000032:analysis*; D014776:Virus Cultivation
D002712:Chlorides; D004279:DNA, Viral; D014764:Viral Proteins; D010772:Phosphotungstic Acid; D002586:Cesium