title: Mode of host cell penetration by bacteriophage phi X174.
abstract: Bacteriophage phiX174 is an icosahedral phage which attaches to host cells without the aid of a complex tail assembly. When phiX174 was mixed with cell walls isolated from the bacterial host, the virions attached to the wall fragments and the phage deoxyribonucleic acid (DNA) was released. Attachment was prevented if the cell walls were treated with chloroform. Release of phage DNA, but not viral attachment, was prevented if the cell walls were incubated with lysozyme or if the virions were inactivated with formaldehyde. Treatment of the cell walls with lysozyme released structures which were of uniform size (6.5 by 25 nm). These structures attached phiX174 at the tip of one of its 12 vertices, but the viral DNA was not released. The virions attached to these structures were oriented with their fivefold axis of symmetry normal to the long axis of the structure. No virions were attached to these structures by more than one vertex. Freeze-etch preparations of phiX174 adsorbed to intact bacteria showed that the virions were submerged to one half their diameter into the host cell wall, and the fivefold axis of symmetry was normal to the cell surface. A second cell could not be attached to the outwardly facing vertex of the adsorbed phage and thus the phage could not cross-link two cells. When the virions were labeled with (3)H-leucine, purified, and adsorbed to Escherichia coli cells, about 15% of the radioactivity was recovered as low-molecular-weight material from spheroplasts formed by lysozyme-ethylenediaminetetraacetic acid. Other experiments revealed that about 7% of the total parental virus protein label could be recovered in newly formed progeny virus.
mesh_terms: D001665:Binding Sites; D002247:Carbon Isotopes; D002473:Cell Wall; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D002725:Chloroform / Q000494:pharmacology; D003090:Coliphages / Q000187:drug effects; D004279:DNA, Viral; D004926:Escherichia coli / Q000166:cytology; D005557:Formaldehyde / Q000494:pharmacology; D005613:Freeze Etching; D005827:Genetics, Microbial; D007930:Leucine; D008242:Lysogeny; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D009113:Muramidase / Q000494:pharmacology; D009154:Mutation; D013194:Staining and Labeling; D013395:Sucrose; D013936:Thymidine; D014316:Tritium; D014764:Viral Proteins
chemical_list: D002247:Carbon Isotopes; D002712:Chlorides; D004279:DNA, Viral; D014764:Viral Proteins; D014316:Tritium; D005557:Formaldehyde; D002586:Cesium; D013395:Sucrose; D002725:Chloroform; D009113:Muramidase; D007930:Leucine; D013936:Thymidine