Isolation of cuticle and vascular bundles of leaves by maceration in rumen fluid.
D000818:Animals; D001826:Body Fluids; D002021:Buffers; D002245:Carbon Dioxide; D002417:Cattle; D002482:Cellulose / Q000378:metabolism; D006652:Histological Techniques; D008855:Microscopy, Electron, Scanning; D059828:Plant Cells; D012417:Rumen / Q000382:microbiology*; D013194:Staining and Labeling; D013696:Temperature; D013997:Time Factors
D002021:Buffers; D002245:Carbon Dioxide; D002482:Cellulose