Enzymic removal and re-expression of a histocompatibility antigen, HL-A 2, at the surface of human peripheral lymphocytes ( 31 Cr-labeled cells-papain-puromycin-actinomycin D).
The removal by papain of a histocompatibility antigen, HL-A 2, from the surface of intact peripheral blood lymphocytes has been examined. Up to 80% of the antigenic determinant can be removed within 90 minutes. If the cells are reincubated in nutritive medium, re-expression of HL-A 2 is complete within 6 hours. Re-expression is reversibly inhibited by puromycin (50 mug/ml), but actinomycin D (6 mug/ml) only partially inhibited re-expression after 2 hours of prior incubation with the drug.
D000941:Antigens / Q000096:biosynthesis; D001798:Blood Proteins / Q000096:biosynthesis; D002859:Chromium Isotopes; D003470:Culture Media; D003609:Dactinomycin / Q000494:pharmacology; D000939:Epitopes; D006648:Histocompatibility; D006801:Humans; D007106:Immune Sera; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D010206:Papain / Q000494:pharmacology*; D011691:Puromycin / Q000494:pharmacology; D013045:Species Specificity; D013997:Time Factors
D000941:Antigens; D001798:Blood Proteins; D002859:Chromium Isotopes; D003470:Culture Media; D000939:Epitopes; D007106:Immune Sera; D003609:Dactinomycin; D011691:Puromycin; D010206:Papain