Structure and infectivity of picornaviral RNA encapsidated by cowpea chlorotic mottle virus protein.
Poliovirus and Mengo virus RNA were shown to associate efficiently with cowpea chlorotic mottle virus protein to form pseudovirions. The sedimentation coefficient for the pseudovirions was similar to that of poliovirus, and electron microscope observations showed the Mengo pseudovirions to be similar in size to Mengo virus. Such pseudovirions were infectious and were more resistant to ribonuclease than viral RNA; however, under our assay conditions, their infectivity was about equal to that of viral RNA.
D000818:Animals; D002499:Centrifugation, Density Gradient; D006367:HeLa Cells; D006801:Humans; D007739:L Cells; D008574:Mengovirus; D051379:Mice; D008854:Microscopy, Electron; D008970:Molecular Weight; D009698:Nucleoproteins / Q000494:pharmacology; D010942:Plant Viruses; D017955:Poliovirus; D012367:RNA, Viral / Q000494:pharmacology*; D013194:Staining and Labeling; D014316:Tritium; D014529:Uridine; D014764:Viral Proteins / Q000494:pharmacology; D014776:Virus Cultivation
D009698:Nucleoproteins; D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium; D014529:Uridine