title: Biochemical differentiation in reaggregating brain cell culture.
abstract: Dissociated cells from embryonic mouse brain reassociate in rotation culture to form aggregates. During cell culture the specific activities of choline acetyl-transferase (EC 2.3.1.6), acetylcholinesterase (EC 3.1.1.7), and glutamate decarboxylase (EC 4.1.1.15) in the aggregates increase up to twenty-fold, a phenomenon that approximates some of the biochemical events in the development of the mouse brain.
mesh_terms: D000110:Acetylcholinesterase / Q000032:analysis; D000217:Acyltransferases / Q000032:analysis; D000367:Age Factors; D000818:Animals; D001921:Brain / Q000166:cytology* / Q000196:embryology / Q000201:enzymology; D002247:Carbon Isotopes; D002262:Carboxy-Lyases / Q000032:analysis; D002449:Cell Aggregation; D002460:Cell Line; D002478:Cells, Cultured / Q000201:enzymology; D002794:Choline; D002999:Clone Cells; D006224:Cricetinae; D005260:Female; D005971:Glutamates; D006651:Histocytochemistry; D051379:Mice; D009447:Neuroblastoma / Q000201:enzymology; D010053:Ovary / Q000201:enzymology; D013194:Staining and Labeling; D013997:Time Factors
chemical_list: D002247:Carbon Isotopes; D005971:Glutamates; D000217:Acyltransferases; D000110:Acetylcholinesterase; D002262:Carboxy-Lyases; D002794:Choline