title: Specific and nonspecific staining in detecting staphylococcal alpha toxin on erythrocytes by immunofluorescence.
abstract: Specifically stained staphylococcal alpha toxin particles were detected on unstained and nonspecifically stained ghost membranes when rabbit erythrocytes were exposed to alpha toxin and stained with fluorescein isothiocyanate-labeled specific antitoxin. Fading of the nonspecific staining was observed and was attributed to the degradation of the membranes by alpha toxin.
mesh_terms: D000818:Animals; D000992:Antitoxins / Q000032:analysis; D004912:Erythrocytes / Q000032:analysis* / Q000276:immunology; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D006461:Hemolysis; D011817:Rabbits / Q000276:immunology; D013194:Staining and Labeling; D013210:Staphylococcus / Q000032:analysis*; D013696:Temperature; D013861:Thiocyanates; D014118:Toxins, Biological / Q000032:analysis*
chemical_list: D000992:Antitoxins; D005452:Fluoresceins; D013861:Thiocyanates; D014118:Toxins, Biological