Isolation and characterization of C-type viral gene products of virus-negative mouse cells.
Antigens which immunologically cross-react with two mouse C-type viral polypeptides, p30 and p12, are present at very low levels in normal virus-negative mouse cells. These two antigens have been purified by 50-300-fold from cell extracts and shown to cochromatograph with the corresponding labeled viral polypeptides in several systems. Their type-specific antigenicities are shown to be distinct from those of previously tested MuLV isolates suggesting that they may be components of a new class of endogenous C-type virus. The methods utilized in the present studies for concentration of virus-specific antigens of normal mouse cells provide an approach for detection of C-type viral antigens in cells of other species.
D000818:Animals; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000145:classification / Q000302:isolation & purification*; D002460:Cell Line; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003429:Cross Reactions; D000939:Epitopes; D007457:Iodine Radioisotopes; D009052:Leukemia Virus, Murine / Q000276:immunology; D008099:Liver; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D011863:Radioimmunoassay; D012190:Retroviridae / Q000276:immunology*; D013154:Spleen; D014764:Viral Proteins / Q000145:classification / Q000302:isolation & purification*; D014779:Virus Replication
D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D007457:Iodine Radioisotopes; D014764:Viral Proteins