Deoxyribonucleic acid polymerase activities in normal and leukovirus-infected chicken embryo cells.
Chicken embryo cells normally contain, in addition to deoxyribonucleic acid (DNA)-dependent DNA (D-DNA) polymerases, a novel "R-DNA-polymerase" which specifically copies polyriboadenylic acid strands. This R-DNA polymerase cannot copy natural ribonucleic acid or polyribocytidylic acid strands to a significant extent. Infection of cells with the leukovirus RAV-2 leads to the intracellular formation of large amounts of the viral RNA-dependent DNA polymerase whose properties differ from the cell R-DNA polymerase. Chicken cells transformed by a Rous sarcoma virus mutant which produce noninfectious alpha-type Rous sarcoma virus (f), a leukovirus known to be deficient in the viral RNA-dependent DNA polymerase, do not contain detectable viral RNA-dependent DNA polymerase, whereas the cellular R-DNA polymerase is found in normal amounts. There seems to be no relationship between the cellular R-DNA polymerase and the RNA-dependent DNA polymerase of the avian leukoviruses.
D000818:Animals; D001354:Avian Leukosis Virus / Q000254:growth & development*; D001358:Avian Sarcoma Viruses / Q000201:enzymology / Q000254:growth & development; D002467:Cell Nucleus / Q000201:enzymology; D002471:Cell Transformation, Neoplastic; D002474:Cell-Free System; D002478:Cells, Cultured / Q000201:enzymology / Q000382:microbiology; D002642:Chick Embryo / Q000382:microbiology*; D002848:Chromatography, DEAE-Cellulose; D003593:Cytoplasm / Q000201:enzymology; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D003673:Defective Viruses / Q000201:enzymology / Q000254:growth & development*; D003854:Deoxyribonucleotides / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013698:Templates, Genetic; D014316:Tritium
D003854:Deoxyribonucleotides; D014316:Tritium; D008345:Manganese; D004254:DNA Nucleotidyltransferases; D012194:RNA-Directed DNA Polymerase; D008274:Magnesium