The distribution of surface antigens during fractionation of mouse liver plasma membranes.
1. Antiserum to purified mouse liver plasma membranes was prepared and the partially purified gamma-globulin antibody fraction was iodinated with (125)I. The reaction of the (125)I-labelled gamma-globulin antibody with isolated mouse liver plasma membranes was studied. 2. The gammaglobulin antibody bound specifically to mouse liver plasma membranes and there was little reaction with mouse liver intracellular membranes or with surface-membrane fractions from either rat liver or pig lymphocytes. 3. ;Light' and ;heavy' mouse liver plasma-membrane subfractions bound similar amounts of gamma-globulin antibody, and this is consistent with a surface origin for the light fraction. 5. Plasma membranes were fractionated by sequential extraction with 50mm-NaHCO(3)-Na(2)CO(3) buffer, pH10.2, containing 10mm-EDTA and aq. 33% (v/v) pyridine. The alkali-soluble and -insoluble fractions and the pyridine-soluble and -insoluble fractions all reacted with the antiserum, and the cross-reactivity among the various fractions and with the total plasma membranes was investigated. 5. The results are discussed in terms of the arrangement of the antigenic determinants within the membrane.
D000818:Animals; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000302:isolation & purification*; D002462:Cell Membrane / Q000276:immunology*; D002501:Centrifugation, Zonal; D007106:Immune Sera; D007456:Iodine Isotopes; D008099:Liver / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D011485:Protein Binding; D011817:Rabbits / Q000276:immunology; D013347:Subcellular Fractions / Q000276:immunology; D005719:gamma-Globulins
D000941:Antigens; D007106:Immune Sera; D007456:Iodine Isotopes; D005719:gamma-Globulins