Isolation and characterization of two glycoproteins from patients with alveolar proteinosis.
The saline-insoluble particulate material obtained by pulmonary lavage from four patients with alveolar proteinosis was analyzed for total lipid (52%), protein (44%), and carbohydrate (4%). Sodium dodecyl sulfate-gel electrophoresis revealed only three major peptides in these patients. Molecular weights (from the gels) were 69,000, 62,000, and 36,000, and the latter two peptides were periodic acid-Schiff stain-positive. Amino-acid and sugar analyses were performed on the peptides cut from the gels and on peptides purified by Sephadex G-200 chromatography. The 69,000-molecular-weight peptide contained no carbohydrate. Antibody studies and aminoacid analysis indicated that it was albumin. The 62,000-molecular-weight peptide contained 1% hydroxyproline, 1% hydroxylysine, 10% glycine, and 9% carbohydrate. The 36,000-molecular-weight peptide contained 1.2% hydroxyproline, 1% hydroxylysine, 13% glycine, 1.4% sialic acid, 2.6% glucose, 2.4% galactose, 2% mannose, 0.8% fucose, 0.4% glycosamine, and 0.6% galactosamine. Proteins extracted from kidney glomeruli with similar amino-acid and carbohydrate composition have been observed previously.
D000596:Amino Acids / Q000032:analysis; D000937:Antigen-Antibody Reactions; D002241:Carbohydrates / Q000032:analysis; D002850:Chromatography, Gel; D004586:Electrophoresis; D000939:Epitopes; D006023:Glycoproteins / Q000032:analysis / Q000302:isolation & purification*; D006801:Humans; D010455:Peptides / Q000032:analysis; D011649:Pulmonary Alveolar Proteinosis / Q000378:metabolism*
D000596:Amino Acids; D002241:Carbohydrates; D000939:Epitopes; D006023:Glycoproteins; D010455:Peptides