title: Cell-cycle analysis in 20 minutes.
abstract: Mithramycin added to mammalian cells fixed in aqueous ethanol is bound to DNA and fluoresces in direct proportion to the cellular DNA content. Quantitative fluorescence measurement by means of a high-speed flow system instrument provides a rapid method for cell-cycle analysis and for the first time permits continuous monitoring of cell-cycle kinetics during ongoing experiments.
mesh_terms: D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured / Q000032:analysis*; D006224:Cricetinae; D003584:Cytological Techniques; D004247:DNA / Q000032:analysis*; D004261:DNA Replication; D005260:Female; D005453:Fluorescence; D005470:Fluorometry; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008168:Lung / Q000196:embryology; D008722:Methods; D008938:Mitosis; D010053:Ovary; D008926:Plicamycin; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling
chemical_list: D004247:DNA; D008926:Plicamycin