Binding of thymus- and bone marrow-derived lymphoid cells to antigen-derivatized fibers.
Thymus-derived lymphocytes (T cells) and bone marrow-derived lymphocytes (B cells) from mouse spleens bind specifically to antigen-derivatized nylon fibers. The fiber-bound population consisted of about 60%-70% B cells and 30% T cells as determined by cytotoxicity, fluorescence, and antibody-complement binding assays. Essentially all fiber-bound cells were viable and could be accounted for as T or B cells. Enriched populations of T or B cells could be isolated on the fibers by destruction of one or the other cell type with the appropriate antiserum plus complement. T or B cells could also be fractionated according to their relative affinity (or avidity) for a given antigen.
D000818:Animals; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000941:Antigens; D001402:B-Lymphocytes / Q000276:immunology*; D001666:Binding Sites, Antibody; D003168:Complement Fixation Tests; D003601:Cytotoxicity Tests, Immunologic; D004140:Dinitrophenols; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D007114:Immunization; D051379:Mice; D008806:Mice, Inbred AKR / Q000276:immunology; D008810:Mice, Inbred C57BL / Q000276:immunology; D009757:Nylons; D012710:Serum Albumin, Bovine; D012756:Sheep / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000941:Antigens; D004140:Dinitrophenols; D000939:Epitopes; D007106:Immune Sera; D009757:Nylons; D012710:Serum Albumin, Bovine