title: A simple freeze-substitution method for the study of ultrastructure of plant tissues.
mesh_terms: D002462:Cell Membrane; D002467:Cell Nucleus; D003593:Cytoplasm; D004721:Endoplasmic Reticulum; D005615:Freezing; D006056:Golgi Apparatus; D006651:Histocytochemistry; D006652:Histological Techniques; D008722:Methods; D008854:Microscopy, Electron; D008870:Microtubules; D009992:Osmium; D059828:Plant Cells; D012440:Saccharomyces / Q000166:cytology; D012441:Saccharomyces cerevisiae / Q000166:cytology; D013194:Staining and Labeling; D003313:Zea mays / Q000166:cytology
chemical_list: D009992:Osmium