Anti-mitotic activity of bleomycin: time of action in the mammalian cell cycle.
Bleomycin inhibits cell division in HeLa S-3 and other mammalian epithelial cell lines. Its effect on suspension cultured cells is far greater than on monolayer cells; this was found to be due partly to the absence of divalent ions. Other mammalian epithelial lines in suspension culture are very sensitive but fibroblastic cells (BHK 21/C13/DWS-3) grown under identical conditions are relatively insensitive. This study has established that bleomycin is a powerful anti-mitotic agent during the G(2) phase but it does not prevent prophase cells from completing a normal mitotic division. Its ability to suppress DNA synthesis contributes little to the inhibition of cell cycling.
D000818:Animals; D001761:Bleomycin / Q000494:pharmacology*; D002250:Carbon Radioisotopes; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002999:Clone Cells / Q000187:drug effects; D006224:Cricetinae; D003562:Cytidine / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007668:Kidney; D008938:Mitosis / Q000187:drug effects*; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D013936:Thymidine; D013997:Time Factors; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D002250:Carbon Radioisotopes; D014316:Tritium; D001761:Bleomycin; D003562:Cytidine; D012313:RNA; D004247:DNA; D013936:Thymidine; D014529:Uridine