Interaction of aggregated  -globulin with B lymphocytes.
Specific binding of aggregated gamma-globulin to a subpopulation of lymphocytes was demonstrated. This subpopulation was identified as the Ig-staining or B lymphocytes. The binding was irreversible and independent of complement, pH, temperature, protein content of the medium, and divalent cations. Aggregates of large size were needed for optimal visualization. Evidence was obtained that the site on the lymphocyte membrane responsible for binding aggregates was distinct from surface Ig.
D002462:Cell Membrane / Q000276:immunology*; D006358:Hot Temperature; D006801:Humans; D007111:Immunity, Cellular; D007074:Immunoglobulin G; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000276:immunology*; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D011485:Protein Binding; D011489:Protein Denaturation; D005719:gamma-Globulins
D007074:Immunoglobulin G; D005719:gamma-Globulins