A type-C virus in human rhabdomyosarcoma cells after inoculation into NIH Swiss mice treated with antithymocyte serum.
A type-C RNA virus has been isolated that replicates readily in human and other primate cells. It was obtained from a human rhabdomyosarcoma cell (RD) that had been serially transplanted in immunosuppressed NIH Swiss mice, a strain of mouse from which infectious type-C virus has not been isolated. Various other human tumor cells, similarly transplanted, remained free of overt type-C virus expression. The virus growing in the RD cells, AT-124, has a group-specific antigen and an RNA-dependent DNA polymerase immunologically related to murine type-C viruses, but a host range similar to that of the RD-114 virus. The new isolate is either a previously undescribed, endogenous type-C virus from NIH Swiss mice or a recombinant with both mouse and human type-C genetic information.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D000961:Antilymphocyte Serum; D002415:Cats; D002460:Cell Line; D000882:Haplorhini; D006801:Humans; D007165:Immunosuppression Therapy; D051379:Mice; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000382:microbiology; D009858:Oncogenic Viruses / Q000302:isolation & purification*; D012328:RNA Viruses / Q000302:isolation & purification*; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D051381:Rats; D012190:Retroviridae / Q000201:enzymology / Q000276:immunology / Q000302:isolation & purification; D012208:Rhabdomyosarcoma / Q000276:immunology / Q000382:microbiology*; D014779:Virus Replication
D000956:Antigens, Viral; D000961:Antilymphocyte Serum; D012194:RNA-Directed DNA Polymerase