Further characterization of a factor from endotoxin-treated serum which releases histamine and heparin from mast cells.
Upon incubation of hamster serum with bacterial endotoxin, a factor is produced which releases histamine and heparin from hamster mast cells and increases capillary permeability in guinea pig skin. The major histamine-releasing activity derived from hamster serum was characterized by gel filtration, found to have a molecular weight of approximately 60,000, and shown by electrophoresis to migrate with alpha-2- or beta-1-globulins. The ability to increase vascular permeability was not reversed by antihistamine. On the basis of these properties, the histamine-liberating factor generated by endotoxin in hamster serum differed significantly from known anaphylatoxins.
D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D006224:Cricetinae / Q000276:immunology; D004590:Electrophoresis, Paper; D004731:Endotoxins / Q000494:pharmacology*; D005260:Female; D006168:Guinea Pigs; D006493:Heparin / Q000032:analysis / Q000378:metabolism*; D006632:Histamine / Q000032:analysis; D006636:Histamine Release / Q000187:drug effects*; D007106:Immune Sera; D007122:Immunoelectrophoresis; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000187:drug effects*; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000494:pharmacology; D012485:Salmonella typhi; D012869:Skin Absorption / Q000187:drug effects; D013395:Sucrose
D004731:Endotoxins; D007106:Immune Sera; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D013395:Sucrose; D006632:Histamine; D006493:Heparin