Group B arbovirus structural and nonstructural antigens. II. Purification of Saint Louis encephalitis virus intracellular antigens.
Three serologically distinct antigens were identified in homogenates of Saint Louis encephalitis (SLE) virus-infected cells after Brij-58 solubilization and diethylaminoethyl (DEAE)-cellulose chromatography. These antigens were designated as antigen I, II, and III. Immunodiffusion analyses showed that all antigen peaks which eluted from the DEAE-cellulose column contained the intracellular major envelope protein, antigen I. This protein was the only viral antigen which eluted at 0.125 M KCl in DEAE-cellulose column peak C. Optical density peak D, which eluted at 0.2 M KCl, contained both antigens I and III. Antigen III was purified from this column fraction approximately 200-fold by organic solvent extraction and chromatography on hydroxylapatite and Sepharose 6B. Purified antigen III had a molecular weight of 80,000 to 85,000 and contained only one antigenic determinant as judged by immunodiffusion analysis.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis / Q000302:isolation & purification*; D002250:Carbon Radioisotopes; D002845:Chromatography; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003168:Complement Fixation Tests; D046508:Culture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D004665:Encephalitis Virus, St. Louis / Q000032:analysis / Q000276:immunology; D004668:Encephalitis Viruses / Q000276:immunology*; D000939:Epitopes; D006882:Hydroxyapatites; D005779:Immunodiffusion; D007668:Kidney; D007930:Leucine; D008970:Molecular Weight; D013501:Surface-Active Agents; D013552:Swine; D014316:Tritium; D014764:Viral Proteins / Q000032:analysis; D014776:Virus Cultivation
D000956:Antigens, Viral; D002250:Carbon Radioisotopes; D000939:Epitopes; D006882:Hydroxyapatites; D013501:Surface-Active Agents; D014764:Viral Proteins; D014316:Tritium; D007930:Leucine