Fractionation of the nucleus by divalent cations. Isolation of nuclear membranes.
0.3-0.5 M MgCl(2) was used to disassemble nuclei and to isolate by a single centrifugation in less than 3 hr a nuclear envelope fraction in 55-60% yield as assessed by phospholipid recovery. Its gross chemical composition was determined and its morphology was studied electron microscopically by sectioning, freeze etching, and negative staining procedures.
D000818:Animals; D002413:Cations, Divalent; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000032:analysis; D002498:Centrifugation; D004247:DNA / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005613:Freeze Etching; D008099:Liver; D008274:Magnesium / Q000494:pharmacology*; D008566:Membranes; D008854:Microscopy, Electron; D008862:Microsomes, Liver / Q000032:analysis; D009698:Nucleoproteins / Q000032:analysis; D010455:Peptides / Q000032:analysis; D011189:Potassium Chloride / Q000494:pharmacology; D012313:RNA / Q000032:analysis; D051381:Rats; D012967:Sodium Dodecyl Sulfate; D013194:Staining and Labeling
D002413:Cations, Divalent; D009698:Nucleoproteins; D010455:Peptides; D012967:Sodium Dodecyl Sulfate; D012313:RNA; D011189:Potassium Chloride; D004247:DNA; D008274:Magnesium