Studies on the adsorption of certain medium proteins to Mycoplasma gallisepticum and their influence on agglutination and haemagglutination reactions.
Serum proteins from Mycoplasma gallisepticum culture medium could be detected on the organisms as a result of incubation at low pH. Only certain of the serum proteins, including IgG and IgM, were found, and the adsorption appears to depress the haemagglutinating activity of the organisms. There was no obvious effect on slide agglutination (SA) sensitivity but an incidental finding was that brief acid treatment enhanced the SA sensitivity of an antigen prepared from a young culture.
D000327:Adsorption; D000371:Agglutination / Q000187:drug effects; D000818:Animals; D000942:Antigens, Bacterial; D001798:Blood Proteins / Q000378:metabolism* / Q000494:pharmacology; D002645:Chickens; D003470:Culture Media; D006384:Hemagglutination / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000032:analysis; D007136:Immunoglobulins / Q000032:analysis; D009174:Mycoplasma / Q000276:immunology*; D011817:Rabbits; D013552:Swine; D014168:Transferrin / Q000032:analysis; D014422:Turkeys; D005719:gamma-Globulins / Q000032:analysis
D000942:Antigens, Bacterial; D001798:Blood Proteins; D003470:Culture Media; D007106:Immune Sera; D007074:Immunoglobulin G; D007136:Immunoglobulins; D014168:Transferrin; D005719:gamma-Globulins