Antigenic and biochemical characterization of the C-type particle of the stable porcine kidney cell line PK-15.
The C-type particles observed by electron microscopy in PK-15 cells were demonstrated to have biochemical and biophysical properties associated with the oncornavirus group: density of 1:16 in a sucrose gradient, 70S RNA, and the RNA-dependent DNA polymerase. The group-specific interspecies antigen, gs-3, was not present. Evidence of a latent infection with a porcine parvovirus was also obtained.
D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D012321:DNA-Directed RNA Polymerases; D005455:Fluorescent Antibody Technique; D007668:Kidney; D008854:Microscopy, Electron; D009858:Oncogenic Viruses / Q000276:immunology; D010179:Pancreas / Q000201:enzymology; D010321:Parvoviridae / Q000276:immunology; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D011817:Rabbits / Q000276:immunology; D012190:Retroviridae / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism; D013326:Strontium Radioisotopes; D013552:Swine; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D013326:Strontium Radioisotopes; D014316:Tritium; D012194:RNA-Directed DNA Polymerase; D012321:DNA-Directed RNA Polymerases; D014529:Uridine