title: A distinct class of inducible murine type-C viruses that replicates in the rabbit SIRC cell line.
abstract: The existence of the selectively permissive rabbit cell line SIRC allows definition of a new class of endogenous murine type-C virus. Continuous clonal lines of transformed cells derived from the BALB/c mouse-embryo cell line BALB/3T3 contain at least two distinct classes of endogenous type-C viral genomes. Spontaneously released endogenous viruses grow well on the mouse cell line NIH/3T3 (N-tropic viruses) but not on the rabbit cell line SIRC. Type-C viruses induced by treatment with BrdU grow well on SIRC (S-tropic viruses) but not in NIH/3T3 or BALB/3T3. BrdU-treated AKR mouse-embryo cells also release an S-tropic virus. N-tropic and S-tropic viruses both have the mouse intraspecies gs-1 and viral RNA-directed DNA polymerase antigenic determinants. DNA.RNA hybridization techniques reveal that the two host-range classes of endogenous viruses are only partially related to each other. Cell transformation facilitates the spontaneous release of the N-tropic viruses; treatment with thymidine analogues induces the production of the S-tropic viruses. Thus, the two classes of viral genomes appear to be subject to different cellular controls.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002415:Cats; D002460:Cell Line; D002999:Clone Cells; D003315:Cornea; D005347:Fibroblasts; D000882:Haplorhini; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis; D012194:RNA-Directed DNA Polymerase / Q000032:analysis / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D012190:Retroviridae / Q000145:classification / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification*; D013045:Species Specificity; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014776:Virus Cultivation; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral; D012367:RNA, Viral; D014316:Tritium; D012194:RNA-Directed DNA Polymerase; D013936:Thymidine