Physiological studies of methane- and methanol-oxidizing bacteria: immunological comparison of a primary alcohol dehydrogenase from Methylococcus capsulatus and Pseudomonas sp. M27.
A primary alcohol dehydrogenase was purified from cell extracts of two apparently unrelated microorganisms, namely, Pseudomonas sp. M27 and Methylococcus capsulatus. Rabbit antiserum prepared against the purified enzyme from M. capsulatus revealed distinctive antigenic determinants by quantitative and gel precipitin reactions. Rabbit antiserum to M27 enzyme detected both distinctive and shared antigenic determinants. Certain methane- and methanol-oxidizing bacteria were grouped on the basis of serological cross-reacting enzyme specificities.
D000429:Alcohol Oxidoreductases / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D001419:Bacteria / Q000201:enzymology* / Q000276:immunology / Q000378:metabolism; D002474:Cell-Free System; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D007106:Immune Sera; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000494:pharmacology; D008697:Methane / Q000378:metabolism*; D000432:Methanol / Q000378:metabolism*; D011233:Precipitin Tests; D011549:Pseudomonas / Q000201:enzymology* / Q000276:immunology / Q000378:metabolism; D011817:Rabbits / Q000276:immunology; D013045:Species Specificity; D013053:Spectrophotometry; D014461:Ultracentrifugation
D000939:Epitopes; D007106:Immune Sera; D007074:Immunoglobulin G; D000429:Alcohol Oxidoreductases; D008697:Methane; D000432:Methanol