Photodynamic inactivation of antigenic determinants of single-stranded DNA bacteriophage phi chi 174.
Bacteriophage phiX174 when photodynamically inactivated (i.e., when rendered unable to produce plaques as a result of exposure to visible light in air in the presence of proflavine) progressively lost their capacity to bind efficiently with homologous antiserum. Such loss of serum-blocking power was evident with heat-inactivated but not with UV-irradiated phage. The ability of the phages to adsorb to host cells, however, remained practically unaltered even after photodynamic inactivation. It thus appears that photodynamic damages in the so-called "jacket" component of the phiX174 coat proteins are partly responsible for the loss of plaque-forming ability, whereas the "spikes" are either poor antigens or insensitive to photodynamic treatment.
D000166:Acridines; D003090:Coliphages; D000939:Epitopes; D006462:Hemolytic Plaque Technique; D006358:Hot Temperature; D007106:Immune Sera; D008027:Light; D009500:Neutralization Tests; D010761:Phosphorus Radioisotopes; D014466:Ultraviolet Rays
D000166:Acridines; D000939:Epitopes; D007106:Immune Sera; D010761:Phosphorus Radioisotopes