Properties of maedi nucleic acid and the presence of ribonucleic acid- and deoxyribonucleic acid-dependent deoxyribonucleic acid polymerase in the virions.
Maedi virus contains a ribonucleic acid (RNA) which can be resolved into three major components, namely, 62S, 33S, and 13S, by sucrose gradient centrifugation. The presence of RNA- and deoxyribonucleic acid (DNA)-dependent DNA polymerase in virions of maedi virus was demonstrated. The enzyme product could be converted into acid-soluble form by pancreatic deoxyribonuclease, but was resistant to digestion by pancreatic ribonuclease and to hydrolysis by NaOH.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002831:Choroid Plexus; D046508:Culture Techniques; D004279:DNA, Viral / Q000096:biosynthesis; D006868:Hydrolysis; D011648:Pulmonary Adenomatosis, Ovine / Q000382:microbiology; D012367:RNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D012194:RNA-Directed DNA Polymerase / Q000032:analysis* / Q000378:metabolism; D012260:Ribonucleases; D012756:Sheep; D012972:Sodium Hydroxide; D013936:Thymidine / Q000378:metabolism; D013942:Thymine Nucleotides / Q000378:metabolism; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D014713:Viruses, Unclassified / Q000032:analysis* / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism
D004279:DNA, Viral; D012367:RNA, Viral; D013942:Thymine Nucleotides; D014316:Tritium; D012972:Sodium Hydroxide; D012194:RNA-Directed DNA Polymerase; D012260:Ribonucleases; D013936:Thymidine; D014529:Uridine