New obligate methylotroph.
A new and unique obligate methylotroph was isolated from enrichment cultures with methanol as the sole source of carbon and energy. The organism grows only on methanol and methylamine and not on methane. It does not have a complex intracellular membrane system. (14)C-acetate was assimilated by growing cultures and cell suspensions but was incorporated into only a limited number of cell constituents. (14)C-acetate incorporation was strictly dependent on the oxidation of methanol or methylamine as a source of energy. Extracts had relatively low levels of enzymes of the tricarboxylic acid cycle, and alpha-ketoglutarate dehydrogenase was not detected. Comparisons were made with a facultative methylotroph isolated from the same enrichment cultures. The new obligate methylotroph contained hexose phosphate synthetase, a key enzyme in the ribose phosphate cycle of methyl metabolism.
D000085:Acetates / Q000378:metabolism; D000588:Amines / Q000378:metabolism*; D001345:Autoradiography; D001419:Bacteria / Q000145:classification / Q000166:cytology / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D002247:Carbon Isotopes; D002474:Cell-Free System; D002845:Chromatography; D004586:Electrophoresis; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D007656:Ketoglutaric Acids; D008291:Malate Dehydrogenase / Q000378:metabolism; D008697:Methane / Q000378:metabolism; D000432:Methanol / Q000378:metabolism*; D008744:Methylamines / Q000378:metabolism; D008854:Microscopy, Electron; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D011773:Pyruvates; D013053:Spectrophotometry; D013194:Staining and Labeling; D013385:Succinate Dehydrogenase / Q000378:metabolism
D000085:Acetates; D000588:Amines; D002247:Carbon Isotopes; D007656:Ketoglutaric Acids; D008744:Methylamines; D011773:Pyruvates; D010088:Oxidoreductases; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D013385:Succinate Dehydrogenase; D008697:Methane; D000432:Methanol