title: Structure and immunological specificity of the Streptococcus mutans group b cell wall antigen.
abstract: The Streptococcus mutans group b antigen of strain FA1 has been defined as to chemical composition and immunological specificity. The antigen in cold trichloroacetic acid extracts was fractionated on diethylaminoethyl-Sephadex A-25 at pH 8.5. Two forms were isolated: a polysaccharide and a mucoprotein. The two polymers reacted as a single substance in agar gel diffusion against specific adsorbed FA1 rabbit antisera but were separated by gel immunoelectrophoresis. No reaction with any other S. mutans or streptococcal group sera occurred. Galactose composed about one-third and galactosamine about 3% of the total weight of each polymer. Rhamnose was a major component of the polysaccharide (47%) but was present only in traces in the mucoprotein. The protein content of the latter was about 40%. No significant quantities of glycerol, phosphorus, or muramic acid were present in either case. Pepsin and trypsin had no effect on the serological specificity of the mucoprotein. d-Galactose and d-galactosamine were strong inhibitors (70%) of the precipitin reaction, whereas d-glucose, d-glucosamine, and N-acetyl-d-glucosamine inhibited between 25 and 35%. The results indicate that the antigen is a major antigenic component of the cell wall and that the specificity of the antigen resides in binding sites which contain both d-galactose and d-galactosamine. Agglutination of whole cells by specific group b antiserum indicates the antibody receptor sites of the polysaccharide antigen are at the surface of the streptococcal cell. The mucoprotein, but not the polysaccharide, was released from the cell by lysozyme. Lysis did not occur. The immunological specificity and other characteristics of the antigen establishes it as the identifying antigen of S. mutans group b.
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis* / Q000302:isolation & purification; D002473:Cell Wall / Q000276:immunology; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D003851:Deoxyribonucleases; D000939:Epitopes; D005688:Galactosamine / Q000032:analysis; D005690:Galactose / Q000032:analysis; D005959:Glucosidases; D007106:Immune Sera; D005779:Immunodiffusion; D008247:Lysosomes; D009088:Mucoproteins / Q000032:analysis; D010434:Pepsin A; D011135:Polysaccharides, Bacterial / Q000032:analysis; D011233:Precipitin Tests; D011817:Rabbits / Q000276:immunology; D012210:Rhamnose / Q000032:analysis; D012260:Ribonucleases; D013291:Streptococcus / Q000276:immunology*; D014357:Trypsin
chemical_list: D000942:Antigens, Bacterial; D000939:Epitopes; D007106:Immune Sera; D009088:Mucoproteins; D011135:Polysaccharides, Bacterial; D005688:Galactosamine; D003851:Deoxyribonucleases; D012260:Ribonucleases; D005959:Glucosidases; D014357:Trypsin; D010434:Pepsin A; D012210:Rhamnose; D005690:Galactose