Association of calcium with membranes of squid giant axon: ultrastructure and microprobe analysis.
Giant axons from the squid, Loligo pealei, were fixed in glutaraldehyde and postfixed in osmium tetroxide. Calcium chloride (5 mM/liter) was added to all aqueous solutions used for tissue processing. Electron-opaque deposits were found along the axonal plasma membranes, within mitochondria, and along the basal plasma membranes of Schwann cells. X-ray microprobe analysis (EMMA-4) yielded signals for calcium and phosphorus when deposits were probed, whereas these elements were not detected in the axoplasm.
D000818:Animals; D001369:Axons / Q000032:analysis* / Q000166:cytology; D002118:Calcium / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis; D003593:Cytoplasm / Q000032:analysis; D049832:Decapodiformes; D004577:Electron Probe Microanalysis; D006651:Histocytochemistry; D008854:Microscopy, Electron; D008928:Mitochondria / Q000032:analysis; D010758:Phosphorus / Q000032:analysis; D012583:Schwann Cells / Q000166:cytology; D013057:Spectrum Analysis; D013194:Staining and Labeling
D010758:Phosphorus; D002118:Calcium