Polymerase activity of Pichinde virus.
Pichinde virus, a member of the arenavirus group, was examined for polymerase activity. Purified virus was found to contain RNA-dependent RNA polymerase but not RNA-dependent DNA polymerase activity. Since RNase but neither DNase nor actinomycin D inhibited the endogenous polymerase reaction, RNA of the virus appeared to be used as the template. The divalent cations Mg(2+) and Mn(2+) were required for optimal reactivity. The RNA product was partially resistant to RNase and the resistant portion had a sedimentation coefficient of 22 to 26S in sucrose gradients.
D000818:Animals; D002460:Cell Line; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D006482:Hemorrhagic Fevers, Viral / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D007668:Kidney; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D009693:Nucleic Acid Hybridization; D012328:RNA Viruses / Q000201:enzymology*; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012260:Ribonucleases; D013942:Thymine Nucleotides / Q000378:metabolism; D014316:Tritium; D014500:Uracil Nucleotides / Q000378:metabolism
D012367:RNA, Viral; D013942:Thymine Nucleotides; D014500:Uracil Nucleotides; D014316:Tritium; D008345:Manganese; D012194:RNA-Directed DNA Polymerase; D012321:DNA-Directed RNA Polymerases; D012260:Ribonucleases; D008274:Magnesium