Immunosorbent for the isolation of specific antibodies against mannan: localization of antigens in yeast cell walls.
A procedure is given for the insolubilization of yeast mannan by entrapment in a polyacrylamide gel. Specific anti-mannan antibodies were separated by immunosorption from all other antibodies of a rabbit antiserum against whole, Formalininactivated Candida utilis cells. Colloidal gold coated with the anti-mannan and anti-non-mannan antibody fractions was used for the ultrastructural visualization of cell wall antigens. A control was performed using normal rabbit serum. Inhibition of anti-mannan antibodies was obtained with mannan oligosaccharides, and none of the antibody fractions showed marking of Saccharomyces cerevisiae cell walls.
D000818:Animals; D000908:Antibodies, Fungal; D000918:Antibody Specificity; D000946:Antigens, Fungal; D002175:Candida / Q000276:immunology*; D002473:Cell Wall; D000939:Epitopes; D007106:Immune Sera; D011817:Rabbits / Q000276:immunology; D012441:Saccharomyces cerevisiae / Q000276:immunology*
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D000939:Epitopes; D007106:Immune Sera