Transformation by simian virus 40 of spleen cells from a hyperimmune rabbit: demonstration of production of specific antibody to the immunizing antigen.
Extracts of spleen cells obtained from a rabbit hyperimmunized with Type III pneumococcal vaccine and transformed with simian virus 40, were subjected to agarose gel electrophoresis in the presence of an [(125)I]S3 polysaccharide-protein conjugate. Binding of radioactivity in the gamma globulin region was observed. Extracts and media obtained from labeled cell cultures contained a protein that bound to an S3, but not to an S8 or inactivated S3 immunoadsorbent. After elution, the bound protein showed a single band on isoelectric focusing which corresponded in isoelectric point to one of the serum anti-S3 antibodies of the donor rabbit. These observations strongly suggest that a normal committed lymphoid cell may be brought into continuous culture by virus transformation and yet retain its ability to synthesize specific antibody.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000907:Antibodies, Bacterial / Q000032:analysis*; D000917:Antibody Formation; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D001428:Bacterial Vaccines; D002471:Cell Transformation, Neoplastic; D007114:Immunization; D007457:Iodine Radioisotopes; D007525:Isoelectric Focusing; D011817:Rabbits; D011863:Radioimmunoassay; D013539:Simian virus 40; D013154:Spleen / Q000276:immunology*; D013296:Streptococcus pneumoniae / Q000276:immunology
D000906:Antibodies; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D007457:Iodine Radioisotopes