On the use of affinity chromatography in demonstrating the transfer of thioester-bound D-phenylalanine from the light enzyme of gramicidin S synthetase to an acceptor site for this amino acid on the heavy enzyme.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000591:Amino Acid Isomerases / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism; D001665:Binding Sites; D001692:Biological Transport; D002250:Carbon Radioisotopes; D002846:Chromatography, Affinity; D006096:Gramicidin / Q000096:biosynthesis*; D008024:Ligands; D008956:Models, Chemical; D009097:Multienzyme Complexes / Q000378:metabolism*; D010453:Peptide Synthases / Q000378:metabolism*; D010649:Phenylalanine / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D011485:Protein Binding; D013438:Sulfhydryl Compounds / Q000378:metabolism
D000596:Amino Acids; D002250:Carbon Radioisotopes; D008024:Ligands; D009097:Multienzyme Complexes; D010761:Phosphorus Radioisotopes; D013438:Sulfhydryl Compounds; D006096:Gramicidin; D010649:Phenylalanine; D000255:Adenosine Triphosphate; D000591:Amino Acid Isomerases; D010453:Peptide Synthases