Continuous pH stat assay technique for glutamine and asparagine synthetase enzyme systems, involving ATP conversion to ADP plus Pi and AMP plus PPi, respectively.
D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000641:Ammonia; D001224:Aspartic Acid; D003201:Computers; D011756:Diphosphates / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000032:analysis* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007778:Lactobacillus / Q000201:enzymology*; D008025:Ligases / Q000032:analysis*; D008722:Methods; D010710:Phosphates / Q000378:metabolism; D011522:Protons; D012639:Seeds / Q000201:enzymology*
D011756:Diphosphates; D010710:Phosphates; D011522:Protons; D001224:Aspartic Acid; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000641:Ammonia; D000255:Adenosine Triphosphate; D008025:Ligases; D005974:Glutamate-Ammonia Ligase