Isolation of cyclic AMP and cyclic GMP by thin-layer chromatography. Application to assay of adenylate cyclase, guanylate cyclase, and cyclic nucleotide phosphodiesterase.
D000225:Adenine; D000255:Adenosine Triphosphate; D000262:Adenylyl Cyclases / Q000302:isolation & purification* / Q000378:metabolism; D000496:Alloxan / Q000494:pharmacology; D000818:Animals; D001001:Anura; D002250:Carbon Radioisotopes; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D000242:Cyclic AMP / Q000302:isolation & purification*; D006152:Cyclic GMP / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D005934:Glucagon / Q000494:pharmacology; D006147:Guanine; D006160:Guanosine Triphosphate; D006162:Guanylate Cyclase / Q000302:isolation & purification*; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D008168:Lung / Q000187:drug effects / Q000201:enzymology; D008722:Methods; D010727:Phosphoric Diester Hydrolases / Q000302:isolation & purification* / Q000378:metabolism; D010786:Photoreceptor Cells / Q000201:enzymology; D051381:Rats; D013997:Time Factors; D014316:Tritium
D002250:Carbon Radioisotopes; D014316:Tritium; D006147:Guanine; D000496:Alloxan; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D005934:Glucagon; D000242:Cyclic AMP; D010727:Phosphoric Diester Hydrolases; D000262:Adenylyl Cyclases; D006162:Guanylate Cyclase; D006152:Cyclic GMP; D000225:Adenine