Microinjection of mouse eggs.
A technique has been developed for injecting known amounts of liquids into fertilized one-celled mouse eggs by use of a calibrated ocular micrometer superimposed on the terminal regions of the injection pipette. From 128 pronuclear eggs, each injected with 180 or 770 cubic microns of bovine gamma globulin at a concentration of 25 milligrams per milliliter in citrate-Locke's solution and then transferred to the oviducts of pregnant foster mothers, 18 living fetuses developed; from 74 eggs, each injected with 2730 cubic microns, 5 fetuses survived. The living fetuses that developed from the injected eggs were smaller than normal in 6 of 23 surviving experimental fetuses as contrasted to only 1 of 19 control fetuses.
D000818:Animals; D002417:Cattle; D005260:Female; D005306:Fertilization; D005333:Fetus; D066298:In Vitro Techniques; D007267:Injections / Q000295:instrumentation*; D051379:Mice; D010063:Ovum / Q000637:transplantation; D011247:Pregnancy; D011270:Pregnancy, Animal; D011677:Punctures / Q000295:instrumentation; D014184:Transplantation, Homologous; D005719:gamma-Globulins
D005719:gamma-Globulins