Regulation of the nicotinamide adenine dinucleotide- and nicotinamide adenine dinucleotide phosphate-dependent glutamate dehydrogenases of Saccharomyces cerevisiae.
Saccharomyces cerevisiae contains two distinct l-glutamate dehydrogenases. These enzymes are affected in a reciprocal fashion by growth on ammonia or dicarboxylic amino acids as the nitrogen source. The specific activity of the nicotinamide adenine dinucleotide phosphate (NADP) (anabolic) enzyme is highest in ammonia-grown cells and is reduced in cells grown on glutamate or aspartate. Conversely, the specific activity of the nicotinamide adenine dinucleotide (NAD) (catabolic) glutamate dehydrogenase is highest in cells grown on glutamate or aspartate and is much lower in cells grown on ammonia. The specific activity of both enzymes is very low in nitrogen-starved yeast. Addition of the ammonia analogue methylamine to the growth medium reduces the specific activity of the NAD-dependent enzyme and increases the specific activity of the NADP-dependent enzyme.
D000596:Amino Acids / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism; D001215:Asparaginase / Q000378:metabolism; D001224:Aspartic Acid / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D002474:Cell-Free System; D003470:Culture Media; D005969:Glutamate Dehydrogenase / Q000096:biosynthesis / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism; D008744:Methylamines / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000254:growth & development; D013053:Spectrophotometry
D000596:Amino Acids; D003470:Culture Media; D005971:Glutamates; D008744:Methylamines; D009243:NAD; D001224:Aspartic Acid; D009249:NADP; D002244:Carbon; D000641:Ammonia; D005969:Glutamate Dehydrogenase; D001215:Asparaginase; D009584:Nitrogen