title: Methotrexate binding to dihydrofolate reductase from a methotrexate-resistant strain of Escherichia coli.
mesh_terms: D001665:Binding Sites; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology*; D005492:Folic Acid; D005493:Folic Acid Antagonists; D007700:Kinetics; D008727:Methotrexate / Q000378:metabolism* / Q000494:pharmacology; D009249:NADP; D009994:Osmolar Concentration; D010084:Oxidation-Reduction; D011485:Protein Binding; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013762:Tetrahydrofolate Dehydrogenase / Q000378:metabolism*; D013997:Time Factors
chemical_list: D005493:Folic Acid Antagonists; D009249:NADP; D005492:Folic Acid; D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate