Inheritance of antibody specificity. I. Anti-(4-hydroxy-3-nitrophenyl)acetyl of the mouse primary response.
Our data suggest that fine specificity of antihapten antibodies is a useful Mendelian marker of variable (V) genes. We found that some mouse strains (e.g., C57/BL6) consistently produced heteroclitic anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) antibodies (relative affinity for related (4-hydroxy-5-iodo-3-nitrophenyl)acetyl and (4-hydroxy-3.5-dinitrophenyl)acetyl was always >2) while other strains (e.g., CBA) produced "conventional" anti-NP antibodies (relative affinities were consistently <1). 48 (CBA x C57BL/6)F(1) mice were studied and most of them had heteroclitic anti-NP antibodies. They were backcrossed to the recessive CBA parent, and 87 backcross animals were similarly tested. Those heterozygous for the C57BL/6 heavy (H)-chain allotype were similar to the C57BL/6 and the F(1) mice while mice homozygous for the CBA allotype were indistinguishable from the CBA. Such monogenic inheritance was observed only in the primary response. Predominance of allotype-linked genes in the control of the fine specificity characteristics was confirmed by immunizing selected homozygous mouse strains. These mice contained various mixtures of genes from C57BL, BALB/c, and other strains. Specificity of their anti-NP was exclusively determined by genes linked to the H-chain allotype, no influence could be attributed to other genes including the H-2-linked genes.
D000818:Animals; D000918:Antibody Specificity; D002645:Chickens / Q000276:immunology; D003433:Crosses, Genetic; D000939:Epitopes; D005796:Genes; D008040:Genetic Linkage; D005838:Genotype; D005916:Globulins / Q000276:immunology; D006462:Hemolytic Plaque Technique; D006649:Histocompatibility Antigens; D007114:Immunization; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007519:Isoantigens; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009578:Nitrobenzenes / Q000276:immunology*; D012710:Serum Albumin, Bovine / Q000276:immunology; D013045:Species Specificity
D000939:Epitopes; D005916:Globulins; D006649:Histocompatibility Antigens; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007519:Isoantigens; D009578:Nitrobenzenes; D012710:Serum Albumin, Bovine