Prostaglandin inactivation in guinea-pig lung and its inhibition.
1 Several compounds were tested for their ability to inhibit prostaglandin metabolism in guinea-pig lungs.2 In isolated perfused lungs polyphoretin phosphate (PPP) inhibited prostaglandin metabolism being active at concentrations greater than 100 ng/ml and having an ID(50) of 2.15 mug/ml against prostaglandin E(2) metabolism and 1.55 mug/ml against prostaglandin F(2alpha) metabolism.3 At these doses no antagonism of the actions of prostaglandins was seen.4 Diphloretin phosphate (DPP) was more active on a weight for weight basis and inhibition of inactivation of prostaglandins was seen at concentrations greater than 10 ng/ml, the ID(50) being 0.54 mug/ml against both prostaglandins E(2) and F(2alpha).5 At concentrations greater than 5 mug/ml DPP antagonized the actions of prostaglandin F(2alpha).6 The sulphydryl-binders N-ethyl maleimide (NEM) and sodium p-chloromercuriphenyl sulphonate (PCMS) also inhibited prostaglandin inactivation by guinea-pig isolated lungs.7 All four compounds inhibited prostaglandin metabolism by a crude enzyme preparation from guinea-pig lungs.
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D003864:Depression, Chemical; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008168:Lung / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D009247:NADH, NADPH Oxidoreductases / Q000037:antagonists & inhibitors; D010477:Perfusion; D011121:Polyphloretin Phosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D011453:Prostaglandins / Q000378:metabolism*; D013997:Time Factors
D000893:Anti-Inflammatory Agents; D011453:Prostaglandins; D011121:Polyphloretin Phosphate; D009247:NADH, NADPH Oxidoreductases