Isolation and partial characterization of a temperature-sensitive Escherichia coli mutant with altered glutaminyl-transfer ribonucleic acid synthetase.
A temperature-sensitive mutant of Escherichia coli has been found in which the conditional growth is a result of a thermosensitive glutaminyl-transfer ribonucleic acid synthetase. The corresponding genetic locus glnS is cotransduced with lip. In a strain containing the mutationally altered glutaminyl-transfer ribonucleic acid synthetase, no derepression of the enzyme itself nor of glutamine synthetase was observed.
D000215:Acylation; D000476:Alkanesulfonates; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D002474:Cell-Free System; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D005973:Glutamine; D009153:Mutagens; D009154:Mutation; D012343:RNA, Transfer / Q000378:metabolism; D013696:Temperature; D014161:Transduction, Genetic
D000476:Alkanesulfonates; D009153:Mutagens; D005973:Glutamine; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases; D005974:Glutamate-Ammonia Ligase