Acidaemia produced by spinal stimulation in the pithed rat.
1. Electrical stimulation of the thoraco-lumbar spinal nervous outflow in the pithed rat preparation produces a fall in arterial blood pH.2. A component of the acidaemic response results from stimulation of skeletal muscle, since the acidaemic response is reduced when contractions of muscle are blocked with gallamine.3. The residual acidaemic response in gallamine-treated rats is reduced by adrenalectomy, suggesting that catecholamines liberated from the adrenal medulla may mediate part of the effect.4. Guanethidine reduces the acidaemic response remaining in adrenalectomized, gallamine-treated rats, suggesting that the effect of noradrenaline released from sympathetic nerve endings contributes to the fall in pH.5. Neither phenoxybenzamine nor propanolol alone significantly reduces the acidaemic response in gallamine-treated rats, but a combination of the two antagonists almost abolishes the effect. Hence the acidaemia is mediated through effects of catecholamines on both alpha- and beta-adreno-receptors.6. Since acidaemia affects responses to sympathetic nerve stimulation and sympathomimetic amines, the significance of these observations is discussed with reference to the use of the pithed rat preparation for assessment of drugs affecting adrenergic mechanisms.
D000433:1-Propanol / Q000494:pharmacology; D000138:Acidosis / Q000503:physiopathology*; D000313:Adrenal Medulla / Q000502:physiology; D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D001769:Blood; D002395:Catecholamines / Q000502:physiology; D002818:Cordotomy; D004558:Electric Stimulation; D005260:Female; D005703:Gallamine Triethiodide / Q000494:pharmacology; D006145:Guanethidine / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009411:Nerve Endings / Q000502:physiology; D009638:Norepinephrine / Q000502:physiology; D010643:Phenoxybenzamine / Q000494:pharmacology; D051381:Rats; D013127:Spinal Nerves / Q000502:physiology*; D013564:Sympathetic Nervous System / Q000502:physiology
D000316:Adrenergic alpha-Agonists; D000318:Adrenergic beta-Agonists; D002395:Catecholamines; D010643:Phenoxybenzamine; D000433:1-Propanol; D005703:Gallamine Triethiodide; D009638:Norepinephrine; D006145:Guanethidine