The isocitrate dehydrogenases of Acinetobacter lwoffi. Studies on the regulation of a nicotinamide-adenine dinucleotide phosphate-linked isoenzyme.
Of the two NADP-linked isocitrate dehydrogenases in Acinetobacter lwoffi the higher-molecular-weight form, isoenzyme-II, is reversibly stimulated sixfold by low concentrations of glyoxylate or pyruvate. Kinetic results indicate that this stimulation of activity involves both an increase in V(max.) and a decrease in the apparent K(m) values for substrates, most markedly that for NADP(+). Other changes brought about by glyoxylate or pyruvate include a shift in the pH optimum for activity and an increased stability to inactivation by heat or urea. Mixtures of glyoxylate plus oxaloacetate, known to inhibit isocitrate dehydrogenases from other organisms, produce inhibition of both A. lowffi isoenzymes, and do not reflect the stimulatory specificity of glyoxylate for isoenzyme-II. Isoenzyme-II is also stimulated by AMP and ADP, but the activation by glyoxylate or pyruvate is shown to be quite independent of the adenylate activation. Differential desensitization of the enzyme by urea to the two types of activator further supports the view that the enzyme possesses two distinct allosteric regulatory sites. The metabolic significance of the activations is discussed.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000249:Adenosine Monophosphate / Q000494:pharmacology; D000421:Alcaligenes / Q000187:drug effects / Q000201:enzymology*; D000494:Allosteric Regulation; D004789:Enzyme Activation; D006038:Glyoxylates / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007521:Isocitrate Dehydrogenase / Q000378:metabolism*; D007523:Isocitrates; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D009249:NADP; D010071:Oxaloacetates / Q000494:pharmacology; D011773:Pyruvates / Q000494:pharmacology
D006038:Glyoxylates; D007523:Isocitrates; D007527:Isoenzymes; D010071:Oxaloacetates; D011773:Pyruvates; D000249:Adenosine Monophosphate; D009249:NADP; D000244:Adenosine Diphosphate; D007521:Isocitrate Dehydrogenase