Degradation of ribonucleic acid in mouse fibroblasts treated with actinomycin.
The cellular RNA content of mouse fibroblasts incubated with actinomycin decreases at a rate of about 1 to 1.5 per cent per hour, while DNA and protein content remain unchanged. This degradation affects nuclear and cytoplasmic RNA, ribosomal and soluble RNA. The breakdown products appear quantitatively in the acid-soluble fraction of the cells and the medium. Polynucleotides synthesized a short period (120 minutes) prior to exposure to actinomycin are degraded before those synthesized 8 to 12 hours previously.
D000818:Animals; D002467:Cell Nucleus; D046508:Culture Techniques; D003593:Cytoplasm; D004247:DNA / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology*; D005347:Fibroblasts; D051379:Mice; D014176:Protein Biosynthesis; D012313:RNA / Q000378:metabolism*; D012270:Ribosomes; D014316:Tritium
D014316:Tritium; D003609:Dactinomycin; D012313:RNA; D004247:DNA