title: The effects of iron-limited growth on the reduced nicotinamide-adenine dinucleotide dehydrogenase activity and the membrane proteins of Candida utilis mitochondria.
abstract: 1. The mitochondrial NADH dehydrogenase (EC 1.6.99.3) of Candida utilis exhibited altered properties when the organism was grown under iron-limited conditions. No suitable acceptor was found for assay of this enzyme from iron-limited cells. 2. Mitochondrial membrane proteins from C. utilis were analysed by polyacrylamide-gel electrophoresis. Compared with glycerol-limited cells, iron limitation resulted in the loss of at least two polypeptides from the mitochondrial membrane. 3. Neither of the polypeptides affected by iron limitation was part of a cytochrome, although one of them was part of the mitochondrial NADH dehydrogenase. 4. Non-haem iron of mitochondrial membranes was released in the presence of sodium dodecyl sulphate, and electrophoresis in solutions of this detergent cannot be used directly to identify iron-sulphur proteins. Non-ionic detergents do not release non-haem iron but nor do they provide a satisfactory system for electrophoretic separation.
mesh_terms: D002175:Candida / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D002250:Carbon Radioisotopes; D003580:Cytochromes; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D005990:Glycerol / Q000494:pharmacology; D006128:Growth / Q000187:drug effects*; D006418:Heme; D007501:Iron / Q000494:pharmacology*; D008566:Membranes / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate; D014316:Tritium
chemical_list: D002250:Carbon Radioisotopes; D003580:Cytochromes; D010455:Peptides; D011506:Proteins; D014316:Tritium; D012967:Sodium Dodecyl Sulfate; D006418:Heme; D007501:Iron; D009247:NADH, NADPH Oxidoreductases; D005990:Glycerol