Liver dehydrogenase levels in rainbow trout, Salmo gairdneri, fed cyclopropenoid fatty acids and aflatoxin B1.
Cyclopropenoid fatty acids in the diet of rainbow trout caused significant reductions in liver protein and activity of glucose-6-phosphate dehydrogenase, NADP-linked isocitrate dehydrogenase, lactate dehydrogenase, and malate dehydrogenase. Changes in total activity were usually accompanied by similar changes in specific activity. The activity of glucose-6-phosphate dehydrogenase appeared to be more sensitive to the ingestion of cyclopropenoid fatty acids than the other dehydrogenases studied. Feeding 20 ppb aflatoxin B(1) to rainbow trout did not significantly change the activity of the dehydrogenases except for a small increase in the activity of glucose-6-phosphate dehydrogenase after 21 days of feeding. Relationships of these changes to the cocarcinogenicity of cyclopropenoid fatty acids and the carcinogenicity of aflatoxin are discussed.
D000284:Administration, Oral; D000348:Aflatoxins / Q000008:administration & dosage / Q000378:metabolism*; D000818:Animals; D003521:Cyclopropanes / Q000008:administration & dosage / Q000378:metabolism*; D004032:Diet; D005227:Fatty Acids / Q000008:administration & dosage / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008291:Malate Dehydrogenase / Q000378:metabolism; D009249:NADP / Q000032:analysis; D010088:Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism*; D011506:Proteins / Q000032:analysis; D012487:Salmonidae / Q000378:metabolism*
D000348:Aflatoxins; D003521:Cyclopropanes; D005227:Fatty Acids; D011506:Proteins; D009249:NADP; D010088:Oxidoreductases; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase