title: Evidence for two reduced triphosphopyridine nucleotide binding sites on dihydrofolate reductase from a methotrexate-resistant strain of Escherichia coli.
mesh_terms: D001665:Binding Sites; D002846:Chromatography, Affinity; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000201:enzymology*; D007700:Kinetics; D008433:Mathematics; D008727:Methotrexate; D009249:NADP / Q000378:metabolism; D009994:Osmolar Concentration; D011485:Protein Binding; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013762:Tetrahydrofolate Dehydrogenase / Q000378:metabolism; D013997:Time Factors; D014462:Ultrafiltration
chemical_list: D009249:NADP; D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate