D-alanine oxidase form Escherichia coli: localization and induction by L-alanine.
Dialyzed membranes of Escherichia coli prepared by an ethylenediaminetetraacetic acid-lysozyme method catalyze the oxidation of both l-alanine and d-alanine. The specific activities for the oxidations of both d-alanine and l-alanine are increased fivefold when the cells are grown in the presence of either l-alanine or dl-alanine, but are increased only slightly when grown in the presence of d-alanine. In the dl-alanine-induced system, the specific activities for the oxidations of some other d-amino acids are also raised. dl-alanine also induces two other alanine catabolizing enzymes, alanine dehydrogenase and alanine-glutamate aminotransferase which are found in the "soluble" fraction of lysozyme-treated cells. The oxidations of both l-alanine and d-alanine were associated with the membranes of induced cells. After the membranes were disintegrated by sonic treatment, both l-alanine and d-alanine oxidation catalysts sedimented in a sucrose density gradient together with d-lactate and l-lactate dehydrogenases, apparently as a single multienzyme complex.
D000409:Alanine / Q000378:metabolism; D000410:Alanine Transaminase / Q000378:metabolism; D000594:Amino Acid Oxidoreductases / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D002462:Cell Membrane / Q000201:enzymology; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D003470:Culture Media; D003605:D-Amino-Acid Oxidase / Q000302:isolation & purification / Q000378:metabolism; D004492:Edetic Acid; D004790:Enzyme Induction; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009097:Multienzyme Complexes / Q000378:metabolism; D009113:Muramidase; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D011773:Pyruvates / Q000096:biosynthesis; D013237:Stereoisomerism
D000596:Amino Acids; D003470:Culture Media; D009097:Multienzyme Complexes; D011773:Pyruvates; D004492:Edetic Acid; D007770:L-Lactate Dehydrogenase; D000594:Amino Acid Oxidoreductases; D003605:D-Amino-Acid Oxidase; D000410:Alanine Transaminase; D009113:Muramidase; D005947:Glucose; D000409:Alanine; D005990:Glycerol