Enzymatic synthesis of solid phase-bound DNA sequences corresponding to specific mammalian genes.
With oligo(dT)-cellulose as primer, RNA-dependent DNA polymerase catalyzes the synthesis of cellulose-bound DNA that is complementary to mouse globin mRNA. The resulting cellulose-bound or solid phase complementary DNA hybridizes specifically with globin mRNA and permits the recovery of intact globin mRNA. This simple technique for the synthesis of solid phase-bound complementary DNA provides an additional and convenient method for the purification of specific genetic sequences.
D000818:Animals; D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D001483:Base Sequence; D002482:Cellulose; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000032:analysis / Q000096:biosynthesis*; D005796:Genes; D005914:Globins; D007136:Immunoglobulins; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D051379:Mice; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides; D012333:RNA, Messenger / Q000032:analysis; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D013698:Templates, Genetic; D013942:Thymine Nucleotides
D007136:Immunoglobulins; D007457:Iodine Radioisotopes; D009841:Oligonucleotides; D012333:RNA, Messenger; D013942:Thymine Nucleotides; D005914:Globins; D002482:Cellulose; D004247:DNA; D012194:RNA-Directed DNA Polymerase