Solubilization and polyacrylamide gel electrophoresis of membrane enzymes with detergents.
D000469:Alkaline Phosphatase / Q000302:isolation & purification; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D002794:Choline / Q000031:analogs & derivatives; D003840:Deoxycholic Acid; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology; D004950:Esterases / Q000302:isolation & purification; D005453:Fluorescence; D005966:Glucuronidase / Q000302:isolation & purification; D006023:Glycoproteins / Q000302:isolation & purification; D006026:Glycoside Hydrolases / Q000302:isolation & purification; D006056:Golgi Apparatus / Q000201:enzymology; D007931:Leucyl Aminopeptidase / Q000302:isolation & purification; D008099:Liver / Q000201:enzymology; D008358:Mannose; D008566:Membranes / Q000201:enzymology*; D008722:Methods; D008862:Microsomes, Liver / Q000201:enzymology; D009247:NADH, NADPH Oxidoreductases / Q000302:isolation & purification; D010727:Phosphoric Diester Hydrolases / Q000302:isolation & purification; D011092:Polyethylene Glycols; D051381:Rats; D012967:Sodium Dodecyl Sulfate; D012995:Solubility; D013552:Swine
D003902:Detergents; D006023:Glycoproteins; D003840:Deoxycholic Acid; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols; D009247:NADH, NADPH Oxidoreductases; D004950:Esterases; D000469:Alkaline Phosphatase; D010727:Phosphoric Diester Hydrolases; D006026:Glycoside Hydrolases; D005966:Glucuronidase; D007931:Leucyl Aminopeptidase; D002794:Choline; D008358:Mannose