Inhibition of murine leukemia virus production in chronically infected AKR cells: a novel effect of interferon.
Treatment of AKR cells that had spontaneously become procedures of a murine leukemia virus with a partially purified mouse interferon (> 5 x 10(7) international mouse reference units per mg of protein) inhibited endogenous virus production. This inhibitory effect decreased over a 72-hr period in a manner similar to interferon-induced antiviral activity directed against vesicular stomatitis virus in AKR cells. Despite the inhibitory effect of interferon on infectious murine leukemia virus and viral reverse transcriptase (RNA-dependent DNA polymerase) titers in the culture fluids, intracellular levels of viral groups-specific antigens were significantly increased. These results suggest that interferon treatment in AKR cells inhibited the assembly or release of the virus.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D007372:Interferons / Q000494:pharmacology*; D009052:Leukemia Virus, Murine / Q000201:enzymology / Q000254:growth & development* / Q000276:immunology; D007942:Leukemia, Experimental / Q000382:microbiology*; D051379:Mice; D008806:Mice, Inbred AKR; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013997:Time Factors; D014779:Virus Replication
D000956:Antigens, Viral; D007372:Interferons; D012194:RNA-Directed DNA Polymerase