A simplified assay for coenzyme M (HSCH2CH2SO3). Resolution of methylcobalamin-coenzyme M methyltransferase and use of sodium borohydride.
D001419:Bacteria / Q000032:analysis / Q000201:enzymology*; D001894:Borohydrides; D002250:Carbon Radioisotopes; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003067:Coenzymes / Q000032:analysis*; D004590:Electrophoresis, Paper; D007700:Kinetics; D008345:Manganese; D008745:Methylation; D008780:Methyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D009249:NADP; D010084:Oxidation-Reduction; D013053:Spectrophotometry; D013438:Sulfhydryl Compounds / Q000032:analysis; D013451:Sulfonic Acids / Q000032:analysis*; D013997:Time Factors; D014805:Vitamin B 12
D001894:Borohydrides; D002250:Carbon Radioisotopes; D003067:Coenzymes; D013438:Sulfhydryl Compounds; D013451:Sulfonic Acids; D008345:Manganese; D009249:NADP; D008780:Methyltransferases; D014805:Vitamin B 12