title: Studies on the effect of the carrier molecule on antihapten antibody synthesis. I. Effect of carrier on the nature of the antibody synthesized.
abstract: 1. Fluorescence quenching has been calibrated, by comparison with equilibrium dialysis, for measurement of antibody-hapten association constants with guinea pig antibody to the 2,4-dinitrophenyl (DNP) determinant. The maximum quenching with all sites occupied which gave best agreement with equilibrium dialysis was found to be 100%. 2. The carrier to which the DNP-hapten is coupled influences significantly the amount, class (gamma(1) or gamma(2)), and affinity of the antibody of DNP specificity made by individual guinea pigs. 3. The affinities of gamma(1)- and gamma(2)-antibodies from individual guinea pigs are generally very similar. 4. Precipitin curves with gamma(1)-antibodies show more marked inhibition of precipitation in antigen excess than do precipitin curves with the less charged gamma(2)-antibodies, indicating the importance of nonspecific forces in the precipitation reactions.
mesh_terms: D000614:Aminocaproates / Q000378:metabolism*; D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000917:Antibody Formation; D001769:Blood; D002417:Cattle; D003956:Dialysis; D004140:Dinitrophenols / Q000378:metabolism*; D005453:Fluorescence; D005782:Gels / Q000378:metabolism*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007700:Kinetics; D008239:Lysine / Q000378:metabolism*; D010047:Ovalbumin / Q000378:metabolism*; D011233:Precipitin Tests; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D005719:gamma-Globulins / Q000378:metabolism*
chemical_list: D000614:Aminocaproates; D000888:Antibodies, Anti-Idiotypic; D004140:Dinitrophenols; D005782:Gels; D005719:gamma-Globulins; D012710:Serum Albumin, Bovine; D010047:Ovalbumin; D008239:Lysine