title: In vitro system for production of mouse mammary tumor virus.
abstract: An in vitro system for production, purification, and concentration of mouse mammary tumor virus is described. Monolayer cultures of C(3)H mouse mammary tumor cells propagated at 34 C in roller bottles in the presence of dexamethasone, a glucocorticoid hormone, release B-type particles which possess ribonucleic acid and a ribonucleic acid-dependent deoxyribonucleic acid polymerase. One thousandfold concentration by ultracentrifugation with subsequent gradient fractionation yielded > 7 x 10(10) particles per ml in the 1.16- to 1.18-g/ml region. Mouse mammary tumor virus produced in this system was free of detectable C-type virus.
mesh_terms: D000818:Animals; D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006160:Guanosine Triphosphate / Q000378:metabolism; D007065:Idoxuridine / Q000494:pharmacology; D008325:Mammary Neoplasms, Experimental; D008324:Mammary Tumor Virus, Mouse / Q000254:growth & development* / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D008809:Mice, Inbred C3H; D008854:Microscopy, Electron; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013942:Thymine Nucleotides / Q000378:metabolism; D014316:Tritium; D014461:Ultracentrifugation; D014529:Uridine / Q000378:metabolism; D014776:Virus Cultivation; D014779:Virus Replication / Q000187:drug effects
chemical_list: D013942:Thymine Nucleotides; D014316:Tritium; D003907:Dexamethasone; D006160:Guanosine Triphosphate; D012194:RNA-Directed DNA Polymerase; D007065:Idoxuridine; D014529:Uridine