Evidence of particle-associated RNA-directed DNA polymerase and high molecular weight RNA in human gastrointestinal and lung malignancies.
Previous communications have demonstrated that neoplastic cells of human breast cancers, leukemias, lymphomas, sarcomas, and brain tumors contain particles with similar diagnostic attributes as those found in RNA oncornaviruses. The present paper concerns malignancies of the gastrointestinal and pulmonary systems for which, like brain tumors, no suitable animal model or corresponding virus exists. By means of the simultaneous detection assay, these tumors have been found to contain 70S RNA and RNA-directed DNA polymerase encapsulated in particulate components possessing densities of 1.16-1.17 g/ml. Twelve out of 17 (70%) colon carcinomas, three out of five (60%) gastric carcinomas, all of three rectal carcinomas, and seven out of ten (70%) lung carcinomas contained detectable levels of these virus-like entities. None of the corresponding normal tissues was positive.
D002499:Centrifugation, Density Gradient; D003110:Colonic Neoplasms / Q000382:microbiology; D004195:Disease Models, Animal; D005770:Gastrointestinal Neoplasms / Q000032:analysis / Q000201:enzymology / Q000382:microbiology*; D006801:Humans; D007181:Inclusion Bodies, Viral; D008175:Lung Neoplasms / Q000032:analysis / Q000201:enzymology / Q000382:microbiology*; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D009858:Oncogenic Viruses / Q000032:analysis / Q000201:enzymology; D012328:RNA Viruses / Q000032:analysis; D012367:RNA, Viral / Q000302:isolation & purification*; D012194:RNA-Directed DNA Polymerase / Q000302:isolation & purification*; D012004:Rectal Neoplasms / Q000382:microbiology; D012190:Retroviridae; D013274:Stomach Neoplasms / Q000382:microbiology; D014316:Tritium
D012367:RNA, Viral; D014316:Tritium; D012194:RNA-Directed DNA Polymerase