Localization of Escherichia coli ribosomal proteins S4 and S14 by electron microscopy of antibody-labeled subunits.
Binding sites for antibodies specific for proteins S4 and S14 of the small subunit of E. coli ribosomes have been mapped on the surface of the subunit by electron microscopy. Antibody binding to reconstituted subunits was shown to depend specifically on the presence of E. coli S4 and S14. Anti-S14 IgG was found to bind to a limited region of the ribosome surface. In contrast anti-S4 IgG was found to bind to three separated regions of the ribosome surface, suggesting S4 has an elongated conformation in situ.
D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology; D001666:Binding Sites, Antibody; D000939:Epitopes; D004926:Escherichia coli / Q000648:ultrastructure*; D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D008854:Microscopy, Electron; D012270:Ribosomes / Q000032:analysis* / Q000276:immunology
D001426:Bacterial Proteins; D000939:Epitopes; D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G