Improved techniques for staining and enumerating focus formation in viral infectivity assays.
Improved techniques consisting of glutaraldehyde fixation, Giemsa staining, and colony counter enumeration of foci in viral infectivity assays were compared to microscope scanning and lantern slide projection methods. These techniques broadened the scope of application to potentially hazardous viruses and increased the efficiency of assaying.
D001355:Alpharetrovirus / Q000472:pathogenicity; D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D004396:Coloring Agents; D005976:Glutaral; D008722:Methods; D008853:Microscopy / Q000295:instrumentation*; D013194:Staining and Labeling; D010948:Viral Plaque Assay / Q000592:standards*; D014780:Viruses / Q000472:pathogenicity*
D004396:Coloring Agents; D005976:Glutaral