title: Stimulation of transport into Escherichia coli membrane vesicles by internally generated reduced nictotinamide adenine dinucleotide.
abstract: Membrane vesicles containing internal alcohol dehydrogenase and nicotinamide adenine dinucleotide (NAD) were prepared from Escherichia coli ML308-225. Upon the addition of ethanol, these vesicles respired and transported several amino acids. Transport rates driven by internally generated reduced NAD (NADH) were about 60 to 80% of that stimulated by d-lactate. This transport was inhibited by cyanide and anaerobiosis. Ferricyanide, a nonpermeable electron acceptor, inhibited transport stimulated by external NADH, but not that stimulated by internally generated NADH.
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D000693:Anaerobiosis; D000963:Antimetabolites / Q000494:pharmacology; D001692:Biological Transport; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism*; D004926:Escherichia coli / Q000166:cytology* / Q000201:enzymology; D005292:Ferricyanides / Q000494:pharmacology; D007773:Lactates / Q000378:metabolism; D008854:Microscopy, Electron; D009243:NAD / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D010101:Oxygen Consumption
chemical_list: D000596:Amino Acids; D000963:Antimetabolites; D005292:Ferricyanides; D007773:Lactates; D009243:NAD; D000429:Alcohol Oxidoreductases; D009247:NADH, NADPH Oxidoreductases