title: Staining tissue for light and electron microscopy by bridging metals with multidentate ligands.
abstract: "Osmium black," a pigment very useful for cytological staining in both light and electron microscopy, may be deposited selectively at the tissue-binding sites of other metal ions by bridging OsO(4) to the tissue bound metal ion through a multidentate ligand.
mesh_terms: D000818:Animals; D006834:Hydrazines; D066298:In Vitro Techniques; D007668:Kidney; D008853:Microscopy; D008854:Microscopy, Electron; D009992:Osmium; D051381:Rats; D013194:Staining and Labeling
chemical_list: D006834:Hydrazines; D009992:Osmium