The effects of aflatoxin B 1  in vivo on membrane--ribosome association.
The effects of aflatoxin B(1) on the endoplasmic reticulum of rat liver has been examined in vivo. Electron microscopy has shown a disorganization and degranulation of rough surfaced membrane under these conditions and evidence is presented that this is a primary effect of the toxin, and results from the direct attack of the aflatoxin on the steroid-dependent ribosome binding sites on the membrane. A technique is described by which the presence of degranulated rough membrane may be detected in microsomal preparations.
D000348:Aflatoxins / Q000494:pharmacology*; D000818:Animals; D001665:Binding Sites; D003579:Cytochrome Reductases / Q000032:analysis; D003574:Cytochrome c Group; D004721:Endoplasmic Reticulum / Q000187:drug effects* / Q000201:enzymology; D008099:Liver; D008297:Male; D008854:Microscopy, Electron; D008862:Microsomes, Liver / Q000187:drug effects; D006899:Mixed Function Oxygenases / Q000032:analysis; D009247:NADH, NADPH Oxidoreductases / Q000032:analysis; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D012270:Ribosomes / Q000187:drug effects; D013739:Testosterone
D000348:Aflatoxins; D003574:Cytochrome c Group; D013739:Testosterone; D012313:RNA; D006899:Mixed Function Oxygenases; D009247:NADH, NADPH Oxidoreductases; D003579:Cytochrome Reductases