Surface antigens of mammalian sarcoma virus-transformed nonproducer cells.
In vitro, clonal BALB/3T3 mouse cells were transformed into nonproductive sarcoma cells by Kirsten murine sarcoma virus. The resulting cells are called K-BALB. Similarly, the Kirsten sarcoma virus and woolly monkey sarcoma virus transformed normal rat embryonic kidney (NRK) cells to nonproductive sarcoma cells K-NRK and W-NRK, respectively. The presence of sarcoma cell surface antigen (SCSA) on these three different cell lines has been examined by immunoelectron microscopy using the rabbit anti-K-BALB, anti-K-NRK, and anti-W-NRK sera preabsorbed with normal embryonic rat cells, NRK cells, and BALB/3T3 cells. Four different specific sarcoma cell surface antigens have been demonstrated: SCSAa, an antigen specific to the surface of W-NRK cells; SCSAb, a surface antigen common to K-NRK and W-NRK cells; SCSAc, a surface antigen common to K-BALB and W-NRK cells; and SCSAd, a surface antigen broadly reactive with the rabbit antisera to W-NRK, K-NRK, and K-BALB.
D000818:Animals; D000937:Antigen-Antibody Reactions; D000951:Antigens, Neoplasm / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D002462:Cell Membrane / Q000276:immunology; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002999:Clone Cells; D000939:Epitopes; D017988:Gammaretrovirus; D000882:Haplorhini; D007668:Kidney / Q000196:embryology; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D011817:Rabbits / Q000276:immunology; D012509:Sarcoma / Q000382:microbiology*; D012513:Sarcoma, Experimental / Q000276:immunology*
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D000939:Epitopes