The type-specific substance from Pneumococcus type 13.
1. The type-specific substance, S.13, from Pneumococcus type 13 was subjected to hydrolysis with alkali, followed by enzymic dephosphorylation, to yield a pentasaccharide. 2. The pentasaccharide, corresponding to the dephosphorylated repeating unit of S.13, was shown to be O-beta-d-galactopyranosyl-(1-->4)-O-beta- d-glucopyranosyl-(1-->3)-O-beta-d- galactofuranosyl-(1-->4)-O-2-acetamido-2-deoxy-beta-d- glucopyranosyl-(1-->2)-ribitol. 3. The phosphodiester linkages in S.13 join the hydroxyl group at position 1 of ribitol and the hydroxyl group at position 4 of a galactopyranosyl residue in the next repeating unit. 4. Ester groups, presumably O-acetyl, are located on positions 2 or 3 of most glucopyranosyl residues in S.13. 5. A partial structure for S.13 is proposed.
D002854:Chromatography, Paper; D004590:Electrophoresis, Paper; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006868:Hydrolysis; D007106:Immune Sera; D008956:Models, Chemical; D009844:Oligosaccharides / Q000032:analysis*; D010084:Oxidation-Reduction; D010710:Phosphates / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D012266:Ribose / Q000032:analysis; D013055:Spectrophotometry, Infrared; D013296:Streptococcus pneumoniae / Q000032:analysis*
D007106:Immune Sera; D009844:Oligosaccharides; D010710:Phosphates; D011135:Polysaccharides, Bacterial; D012266:Ribose; D005947:Glucose; D005690:Galactose