Disc electrophoresis of rat plasma lipoproteins.
The disc electrophoresis of lipoproteins of unfractionated rat plasma is described. The plasma was prestained with Sudan black B and electrophoretically separated at polyacrylamide gel concentrations of 7.5, 5, and 3.75%. At least four lipoprotein components were observed, and an additional 2-3 components in the main gel and 2-5 components in the spacer gel possibly were present. Densitometry of the resolved gel patterns indicated good reproducibility. Thin-layer chromatography of lipids extracted from the Sudan black B-binding components confirmed the lipoprotein nature of these components of rat plasma. A comparison of the disc electrophoretic patterns of human serum and rat plasma suggested that the low-density lipoprotein components of rat plasma are smaller in size than those of human serum.
D000818:Animals; D001797:Blood Protein Electrophoresis; D002855:Chromatography, Thin Layer; D003720:Densitometry; D006801:Humans; D066298:In Vitro Techniques; D008074:Lipoproteins; D010783:Photometry; D051381:Rats; D013194:Staining and Labeling; D014461:Ultracentrifugation
D008074:Lipoproteins