Changes in respiratory activities during the cell-cycle of the fission yeast Schizosaccharomyces pompe 972h--growing in the presence of glycerol.
1. The specific activities of cytochrome c oxidase, catalase, succinate dehydrogenase, succinate-cytochrome c oxidoreductase, NADH-cytochrome c oxidoreductase, and NADPH-cytochrome c oxidoreductase in mid-exponential-phase batch cultures of glycerol-grown Schizosaccharomyces pombe indicated that the organisms were catabolite-de-repressed. 2. In cultures growing synchronously in the presence of glycerol as sole carbon source, the respiration rate showed two abrupt increases at about 0.45 and 0.95 of the cell-cycle and remained constant in the periods between successive rises. 3. Catalase, succinate dehydrogenase, NADH-cytochrome c oxidoreductase and acid p-nitrophenyl-phosphatase all showed peak patterns of expression in synchronous cultures. 4. Cytochrome c oxidase and cytochromes a+a(3) both showed step patterns of expression with two rises per cell-cycle. 5. Cytochromes c(548), b(554) and b(560) all followed similar time-courses in step patterns of expression, but these were distinct from, and more complex than, that of cytochromes a+a(3). 6. These results are compared with those previously obtained with glucose-grown cultures, and the part played by catabolite repression in the expression of respiratory activities in the cell-cycle is assessed.
D001203:Ascomycota / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D002455:Cell Division; D003579:Cytochrome Reductases / Q000378:metabolism; D004579:Electron Transport; D003576:Electron Transport Complex IV / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D007700:Kinetics; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D010101:Oxygen Consumption; D012568:Schizosaccharomyces / Q000201:enzymology / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013997:Time Factors
D002374:Catalase; D013385:Succinate Dehydrogenase; D009247:NADH, NADPH Oxidoreductases; D003579:Cytochrome Reductases; D003576:Electron Transport Complex IV; D005990:Glycerol