Differential light scattering from spherical mammalian cells.
The differential scattered light intensity patterns of spherical mammalian cells were measured with a new photometer which uses high-speed film as the light detector. The scattering objects, interphase and mitotic Chinese hamster ovary cells and HeLa cells, were modeled as (a) a coated sphere, accounting for nucleus and cytoplasm, and (b) a homogeneous sphere when no cellular nucleus was present. The refractive indices and size distribution of the cells were measured for an accurate comparison of the theoretical model with the light-scattering measurements. The light scattered beyond the forward direction is found to contain information about internal cellular morphology, provided the size distribution of the cells is not too broad.
D002455:Cell Division; D002460:Cell Line; D002467:Cell Nucleus; D003470:Culture Media; D003593:Cytoplasm; D005260:Female; D006367:HeLa Cells / Q000166:cytology*; D006801:Humans; D008027:Light; D008433:Mathematics; D008938:Mitosis; D008954:Models, Biological; D010053:Ovary / Q000166:cytology*; D010781:Photography; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine; D013194:Staining and Labeling; D013997:Time Factors
D003470:Culture Media; D012710:Serum Albumin, Bovine