Stimulation of Herpesvirus saimiri expression in the absence of evidence for type C virus activation in a marmoset lymphoid cell line.
Nucleic acid base analogues were used to examine a Herpesvirus saimiri (HVS)-infected marmoset lymphoid cell line (MLC-1) for possible association with type C viruses. Synthetic templates poly(rA).d(pT)(10) and poly(dA).d(pT)(10) were used to detect RNA-directed DNA polymerase activity in 100-fold concentrated tissue culture fluids. HVS was monitored by immunofluorescence for early, late, and membrane antigens. MLC-1 cells were exposed to 30 mug of 5-bromo-2'-deoxyuridine (BUdR) per ml for 24 h and examined daily. Similar experiments used 5-iodo-2'-deoxyuridine (IUdR) (20 mug/ml) for 30 h or IUdR (20 mug/ml) for 3 days followed by 2% dimethyl sulfoxide for 4 days. Results of these experiments failed to show any type C virus-like polymerase; however, HVS expression was greatly stimulated. BUdR and IUdR enhanced expression of HVS-associated antigens five- to sevenfold, with maximal stimulation being observed 3 to 4 days after removal of the analogue. IUdR-dimethyl sulfoxide treatment was generally less effective. Although more cells showed HVS antigens, the treatments did not increase cell-free infectious virus. The data suggest that HVS-infected lymphoid cells can be stimulated to express virus in a manner similar to that of the Epstein-Barr virus in Burkitt's lymphoma cells. No evidence of type C virus was found in stimulated cultures.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002460:Cell Line; D002474:Cell-Free System; D005455:Fluorescent Antibody Technique; D000882:Haplorhini; D006564:Herpesviridae / Q000254:growth & development* / Q000276:immunology / Q000302:isolation & purification; D007065:Idoxuridine / Q000494:pharmacology*; D008223:Lymphoma; D011119:Polynucleotides / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012190:Retroviridae / Q000201:enzymology / Q000254:growth & development* / Q000302:isolation & purification; D013698:Templates, Genetic; D014779:Virus Replication
D000956:Antigens, Viral; D011119:Polynucleotides; D012194:RNA-Directed DNA Polymerase; D001973:Bromodeoxyuridine; D007065:Idoxuridine