Enzyme assay in microsomes below zero degrees.
Reactions of a membrane-bound multienzyme complex (electron-transport chain of rat-liver microsomes) suspended in aqueous-organic solvent used as antifreeze at temperatures below 0 degrees were studied. In the presence of a relatively high concentration of ethylene glycol, electron transfer can still be observed and some individual and sequential enzyme assays can be performed over a wide range of temperatures below 0 degrees .
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003579:Cytochrome Reductases / Q000378:metabolism; D003574:Cytochrome c Group / Q000378:metabolism; D004579:Electron Transport; D005030:Ethylenes; D005615:Freezing; D006018:Glycols; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D009097:Multienzyme Complexes / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*; D051381:Rats; D013053:Spectrophotometry
D003574:Cytochrome c Group; D005030:Ethylenes; D006018:Glycols; D009097:Multienzyme Complexes; D009249:NADP; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D003579:Cytochrome Reductases