Distribution of concanavalin a receptors on normal rat liver cells and zajdela ascites hepatoma cells.
The distribution of Concanavalin A receptors in cultures of normal rat liver cells and of Zajdela ascites hepatoma cells of the rat was investigated by the Con A-po technique (Bernhard and Avrameas, 1971). Con A-po labelling of the cell surface showed a patchy distribution of Concanavalin A receptors in ascites hepatoma cells, whereas continuous Con A-po labelling was seen at the surface of normal rat liver cells. The different distribution of Concanavalin A receptors was caused by rapid internalization of Con A-po labelled material and not by a simple rearrangement of the receptors. The significance of this process is discussed with respect to Concanavalin A receptor mobility and membrane stability after cell transformation.
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D003208:Concanavalin A / Q000378:metabolism*; D003470:Culture Media; D006056:Golgi Apparatus / Q000648:ultrastructure; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D008113:Liver Neoplasms; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental; D010544:Peroxidases; D051381:Rats; D011955:Receptors, Drug; D013194:Staining and Labeling; D004124:p-Dimethylaminoazobenzene
D003470:Culture Media; D011955:Receptors, Drug; D003208:Concanavalin A; D004124:p-Dimethylaminoazobenzene; D010544:Peroxidases