title: The effect of histidine modification on the activity of dihydrofolate reductase from a methotrexate-resistant strain of Escherichia coli B.
mesh_terms: D000494:Allosteric Regulation; D000495:Allosteric Site; D000596:Amino Acids / Q000032:analysis; D000812:Anhydrides; D001665:Binding Sites; D002942:Circular Dichroism; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology*; D005019:Ethyl Ethers; D005561:Formates; D006639:Histidine / Q000031:analogs & derivatives / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D006898:Hydroxylamines; D007700:Kinetics; D008024:Ligands; D008727:Methotrexate / Q000494:pharmacology*; D009154:Mutation; D009249:NADP; D010084:Oxidation-Reduction; D011485:Protein Binding; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013762:Tetrahydrofolate Dehydrogenase / Q000378:metabolism; D013997:Time Factors
chemical_list: D000596:Amino Acids; D000812:Anhydrides; D005019:Ethyl Ethers; D005561:Formates; D006898:Hydroxylamines; D008024:Ligands; D006639:Histidine; D009249:NADP; D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate