Physical and chemical properties of an oncornavirus associated with a murine adrenal carcinoma cell line.
A type C oncornavirus has been isolated from a continuous cell line of murine adrenal carcinoma in culture. The particles have a buoyant density of 1.165 g/cm(3), exhibit typical type C morphology by electron microscopy, possess an RNA-dependent DNA polymerase, and have a high molecular weight RNA (6.1 x 10(6)) which can be denatured to a homogeneous lower molecular weight species (3.2 x 10(6)) when extracted from rapidly harvested "immature" virions. The virus is related antigenically to other mammalian oncornaviruses and exhibits a similar, although much more complex, sodium dodecyl sulfate gel electrophoretic profile of virion proteins when compared to the profiles of other type C RNA tumor viruses.
D000310:Adrenal Gland Neoplasms; D000818:Animals; D001354:Avian Leukosis Virus / Q000032:analysis; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D009052:Leukemia Virus, Murine / Q000032:analysis; D008324:Mammary Tumor Virus, Mouse / Q000032:analysis; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D008979:Moloney murine leukemia virus / Q000032:analysis; D009691:Nucleic Acid Denaturation; D009858:Oncogenic Viruses / Q000032:analysis; D012328:RNA Viruses / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D012190:Retroviridae / Q000032:analysis; D012967:Sodium Dodecyl Sulfate; D014764:Viral Proteins / Q000032:analysis
D012367:RNA, Viral; D014764:Viral Proteins; D012967:Sodium Dodecyl Sulfate; D012194:RNA-Directed DNA Polymerase