title: Specificity of the DNA product of RNA-dependent DNA polymerase in type C viruses: 3. Analysis of viruses derived from Syrian hamsters.
abstract: DNA transcripts were prepared from three related viruses of hamster and analyzed by interviral hybridization and by reaction with cellular DNA. A virus (G-HaLV), isolated from a dimethylbenzanthraceneinduced tumor cell in Graffi hamsters, contained nucleic acid sequences highly specific for hamster cell DNA and did not react with mouse cell DNA nor did its transcript show homology (<5%) with mouse or rat viral RNAs. The hamster-specific sarcoma virus, B-34, isolated by Bassin and coworkers from tumors induced by the Harvey strain of murine sarcoma virus, contained mouse-, hamster-, and possibly rat-specific sequences. B-34 transcripts were predominantly mouse-specific. GLOH(-), a lymphomagenic virus derived by dilution beyond the transforming endpoint of a hamster-specific sarcoma virus obtained from tumors induced by the Gross pseudotype of murine sarcoma virus, also contained hamster- and mouse-specific sequences. Only a portion of its hamster sequence (about 50%) was shared with B-34 and G-HaLV viruses. As expected, transcripts of GLOH(-) virus were reactive with mouse and hamster cellular DNA.
mesh_terms: D000818:Animals; D002471:Cell Transformation, Neoplastic; D006224:Cricetinae; D004247:DNA / Q000032:analysis / Q000096:biosynthesis*; D004277:DNA, Single-Stranded / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis*; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D012333:RNA, Messenger; D012367:RNA, Viral / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D012190:Retroviridae / Q000201:enzymology / Q000378:metabolism*; D013045:Species Specificity; D014158:Transcription, Genetic; D014316:Tritium
chemical_list: D004277:DNA, Single-Stranded; D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D014316:Tritium; D004247:DNA; D012194:RNA-Directed DNA Polymerase