title: Genetic mapping of the antigenic determinants of two polysaccharide K antigens, K10 and K54, in Escherichia coli.
abstract: The genes controlling synthesis of the Escherichia coli acidic polysaccharide capsular antigens K10 and K54 were transferred by conjugation to E. coli strains of other serotypes. The genes concerned with these K antigen determinants showed genetic linkage with the serA locus. We propose to name the K antigen-controlling gene kpsA. The genetic determinants of the two K antigens could also be transferred to enteropathogenic serotypes, even though such strains have never been found in nature with special acidic polysaccharide K antigens. A noncapsulated derivative, K(-), of the K10 strain can transfer the genetic determinant of the K antigen, demonstrating the probable existence of another chromosomal locus involved in the production of such acidic polysaccharide K antigens.
mesh_terms: D000942:Antigens, Bacterial; D002874:Chromosome Mapping; D003227:Conjugation, Genetic; D003433:Crosses, Genetic; D000939:Epitopes; D004926:Escherichia coli / Q000201:enzymology / Q000276:immunology* / Q000472:pathogenicity; D005796:Genes; D008040:Genetic Linkage; D007122:Immunoelectrophoresis; D009153:Mutagens; D009154:Mutation; D009604:Nitrosoguanidines; D009608:Nitrous Acid; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D011135:Polysaccharides, Bacterial; D011995:Recombination, Genetic; D012703:Serotyping
chemical_list: D000942:Antigens, Bacterial; D000939:Epitopes; D009153:Mutagens; D009604:Nitrosoguanidines; D011135:Polysaccharides, Bacterial; D010744:Phosphoric Monoester Hydrolases; D009608:Nitrous Acid