In vitro system for production of mouse mammary tumor virus.
An in vitro system for production, purification, and concentration of mouse mammary tumor virus is described. Monolayer cultures of C(3)H mouse mammary tumor cells propagated at 34 C in roller bottles in the presence of dexamethasone, a glucocorticoid hormone, release B-type particles which possess ribonucleic acid and a ribonucleic acid-dependent deoxyribonucleic acid polymerase. One thousandfold concentration by ultracentrifugation with subsequent gradient fractionation yielded > 7 x 10(10) particles per ml in the 1.16- to 1.18-g/ml region. Mouse mammary tumor virus produced in this system was free of detectable C-type virus.
D000818:Animals; D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006160:Guanosine Triphosphate / Q000378:metabolism; D007065:Idoxuridine / Q000494:pharmacology; D008325:Mammary Neoplasms, Experimental; D008324:Mammary Tumor Virus, Mouse / Q000254:growth & development* / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D008809:Mice, Inbred C3H; D008854:Microscopy, Electron; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013942:Thymine Nucleotides / Q000378:metabolism; D014316:Tritium; D014461:Ultracentrifugation; D014529:Uridine / Q000378:metabolism; D014776:Virus Cultivation; D014779:Virus Replication / Q000187:drug effects
D013942:Thymine Nucleotides; D014316:Tritium; D003907:Dexamethasone; D006160:Guanosine Triphosphate; D012194:RNA-Directed DNA Polymerase; D007065:Idoxuridine; D014529:Uridine