The bacterial degradation of flavonoids. Hydroxylation of the A-ring of taxifolin by a soil pseudomonad.
Cell-free extracts prepared from a Pseudomonas sp. grown on (+)-catechin oxidized taxifolin in the presence of NAD(P)H and molecular oxygen. The enzyme catalysing the reaction was partially purified and shown to be a flavoprotein. The product was identified as 3',4',5,7,8-pentahydroxyflavanonol (dihydrogossypetin).
D002396:Catechols / Q000378:metabolism; D002474:Cell-Free System; D002855:Chromatography, Thin Layer; D003470:Culture Media; D004589:Electrophoresis, Disc; D005419:Flavonoids / Q000378:metabolism*; D005420:Flavoproteins / Q000032:analysis; D006900:Hydroxylation; D006899:Mixed Function Oxygenases / Q000032:analysis; D009249:NADP; D010084:Oxidation-Reduction; D010100:Oxygen; D010105:Oxygenases / Q000032:analysis; D011549:Pseudomonas / Q000201:enzymology / Q000378:metabolism*; D012988:Soil Microbiology; D013053:Spectrophotometry
D002396:Catechols; D003470:Culture Media; D005419:Flavonoids; D005420:Flavoproteins; D009249:NADP; D006899:Mixed Function Oxygenases; D010105:Oxygenases; D010100:Oxygen