Measurement of the ATP/ADP ratio in mitochondria and in the extramitochondrial compartment by fractionation of freeze-stopped liver tissue in non-aqueous media.
D000135:Acid Phosphatase / Q000378:metabolism; D000227:Adenine Nucleotides / Q000032:analysis; D000244:Adenosine Diphosphate / Q000032:analysis*; D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D002950:Citrate (si)-Synthase / Q000378:metabolism; D002951:Citrates / Q000378:metabolism; D003470:Culture Media; D003579:Cytochrome Reductases / Q000378:metabolism; D005470:Fluorometry; D005615:Freezing; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000378:metabolism / Q000648:ultrastructure*; D008247:Lysosomes / Q000201:enzymology; D008293:Malates / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D008930:Mitochondria, Liver / Q000032:analysis* / Q000201:enzymology; D009249:NADP; D009713:Nucleotidyltransferases / Q000378:metabolism; D010101:Oxygen Consumption; D010477:Perfusion; D010735:Phosphoglycerate Kinase / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D051381:Rats
D000227:Adenine Nucleotides; D002951:Citrates; D003470:Culture Media; D008293:Malates; D011506:Proteins; D009249:NADP; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase; D005969:Glutamate Dehydrogenase; D003579:Cytochrome Reductases; D002950:Citrate (si)-Synthase; D010735:Phosphoglycerate Kinase; D009713:Nucleotidyltransferases; D000135:Acid Phosphatase