Characteristics of streptococcal group-specific antibody isolated from hyperimmune rabbits.
Intravenous immunization of New Zealand red rabbits with streptococcal group-specific bacterial vaccines yielded sera which possessed markedly elevated gamma-globulin. The sera of one rabbit immunized with Group A-variant vaccine possessed 55 mg/ml of gamma-globulin. The bulk of this gamma-globulin, identified as gamma(G)-globulin, was homogeneous by zone electrophoresis and of specificity directed against the Group A-variant carbohydrate antigen. L chains isolated from specific antibody obtained from an immune precipitate were distributed as a single band on starch gel electrophoresis, whereas the normal gamma-globulin traveled as a diffuse smear. These data suggest that the rabbit streptococcal Group A-variant antibodies possess a limited range of physicochemical properties and electrophoretic mobility compared to that generally observed for the normal complement of gamma-globulin.
D000327:Adsorption; D000818:Animals; D000906:Antibodies; D000917:Antibody Formation; D000941:Antigens; D001428:Bacterial Vaccines / Q000494:pharmacology*; D001798:Blood Proteins; D002850:Chromatography, Gel; D004586:Electrophoresis; D007106:Immune Sera; D007122:Immunoelectrophoresis; D066298:In Vitro Techniques; D011233:Precipitin Tests; D011817:Rabbits; D013291:Streptococcus; D005719:gamma-Globulins / Q000096:biosynthesis*
D000906:Antibodies; D000941:Antigens; D001428:Bacterial Vaccines; D001798:Blood Proteins; D007106:Immune Sera; D005719:gamma-Globulins