Electron microscopy: sodium localization in normal and ouabain-treated transporting cells.
When rabbit corneas are fixed in an osmic acid fixativeto which potassium pyroantimonate has been added, an electron-opaque precipitate of sodiium pyroantimonate is localized along the cellular side of the lateral plasmla membrane of the endothelial cells. This heavy precipitate represents areas of high Na(+) concentration and may represent the site of a saturated, high-affinity Na(+) carrier. Significantly heavier precipitate occurs at the same site when ouabain is injected into the aqueous humor prior to fixation of the cornea.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects*; D011232:Chemical Precipitation; D003315:Cornea / Q000166:cytology* / Q000187:drug effects*; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D010042:Ouabain / Q000494:pharmacology*; D011817:Rabbits; D012964:Sodium; D013194:Staining and Labeling
D010042:Ouabain; D012964:Sodium