Genetic control of immune responses in vitro. V. Stimulation of suppressor T cells in nonresponder mice by the terpolymer L-glutamic acid 60-L-alanine 30-L-tyrosine 10 (GAT).
In recent studies we have found that GAT not only fails to elicit a GAT-specific response in nonresponder mice but also specifically decreases the ability of nonresponder mice to develop a GAT-specific PFC response to a subsequent challenge with GAT bound to the immunogenic carrier, MBSA. Studies presented in this paper demonstrate that B cells from nonresponder, DBA/1 mice rendered unresponsive by GAT in vivo can respond in vitro to GAT-MBSA if exogenous, carrier-primed T cells are added to the cultures. The unresponsiveness was shown to be the result of impaired carrier-specific helper T-cell function in the spleen cells of GAT-primed mice. Spleen cells from GAT-primed mice specifically suppressed the GAT-specific PFC response of spleen cells from normal DBA/1 mice incubated with GAT-MBSA. This suppression was prevented by pretreatment of GAT-primed spleen cells with anti-theta serum plus C or X irradiation. Identification of the suppressor cells as T cells was confirmed by the demonstration that suppressor cells were confined to the fraction of the column-purified lymphocytes which contained theta-positive cells and a few non-Ig-bearing cells. The significance of these data to our understanding of Ir-gene regulation of the immune response is discussed.
D000409:Alanine / Q000276:immunology; D000818:Animals; D000941:Antigens; D001402:B-Lymphocytes / Q000276:immunology; D002469:Cell Separation; D003911:Dextrans; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology; D005838:Genotype; D005971:Glutamates / Q000276:immunology; D007108:Immune Tolerance; D007111:Immunity, Cellular / Q000528:radiation effects; D007114:Immunization; D007140:Immunoglobulin Fab Fragments; D051379:Mice; D008811:Mice, Inbred DBA / Q000276:immunology*; D010567:Pertussis Vaccine; D011830:Radiation Effects; D012756:Sheep / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000528:radiation effects; D014443:Tyrosine / Q000276:immunology
D000941:Antigens; D003911:Dextrans; D000939:Epitopes; D005971:Glutamates; D007140:Immunoglobulin Fab Fragments; D010567:Pertussis Vaccine; D014443:Tyrosine; D000409:Alanine