Analytical study of microsomes and isolated subcellular membranes from rat liver. I. Biochemical methods.
The series introduced by this paper reports the results of a detailed analysis of the microsomal fraction from rat liver by density gradient centrifugation. The biochemical methods used throughout this work for the determination of monoamine oxidase, NADH cytochrome c reductase, NADPH cytochrome c reductase, cytochrome oxidase, catalase, aminopyrine demethylase, cytochromes b(5) and P 450, glucuronyltransferase, galactosyltransferase, esterase, alkaline and acid phosphatases, 5'-nucleotidase, glucose 6-phosphatase, alkaline phosphodiesterase I, N-acetyl-beta-glucosaminidase, beta-glucuronidase, nucleoside diphosphatase, aldolase, fumarase, glutamine synthetase, protein, phospholipid, cholesterol, and RNA are described and justified when necessary.
D000135:Acid Phosphatase / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001322:Autoanalysis; D002374:Catalase / Q000032:analysis; D002458:Cell Fractionation; D003579:Cytochrome Reductases / Q000032:analysis; D004950:Esterases / Q000032:analysis; D005634:Fructose-Bisphosphate Aldolase / Q000032:analysis; D005952:Glucose-6-Phosphatase / Q000032:analysis; D014453:Glucuronosyltransferase / Q000032:analysis; D005974:Glutamate-Ammonia Ligase / Q000032:analysis; D006602:Hexosyltransferases / Q000032:analysis; D008099:Liver / Q000032:analysis / Q000166:cytology*; D008566:Membranes / Q000201:enzymology; D008862:Microsomes, Liver / Q000201:enzymology*; D008995:Monoamine Oxidase / Q000032:analysis; D009708:Nucleotidases / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D010727:Phosphoric Diester Hydrolases / Q000032:analysis; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis; D011506:Proteins / Q000032:analysis; D012313:RNA / Q000032:analysis; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology
D010743:Phospholipids; D011506:Proteins; D012313:RNA; D002374:Catalase; D008995:Monoamine Oxidase; D003579:Cytochrome Reductases; D006602:Hexosyltransferases; D014453:Glucuronosyltransferase; D004950:Esterases; D009708:Nucleotidases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D010744:Phosphoric Monoester Hydrolases; D005952:Glucose-6-Phosphatase; D010727:Phosphoric Diester Hydrolases; D005634:Fructose-Bisphosphate Aldolase; D005974:Glutamate-Ammonia Ligase