title: Regulation of gene function: a comparison of X-linked enzyme activity levels in normal and intersexual triploids of Drosophila melanogaster.
abstract: We have measured gene function in normal and male-like intersexual triploids of Drosophila melanogaster by assaying crude extracts of whole flies or thoraces for levels of an X-linked (6-phosphogluconate dehydrogenase) and an autosomal (NADP-dependent isocitrate dehydrogenase) enzyme activity. Our observations lead us to conclude that each dose of the X-linked gene is more active in the cells of these intersexes than it is in normal triploid or diploid female cells. These results indicate that a level of activity intermediate between the normal male and female levels is possible for X-linked genes.
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000201:enzymology; D005260:Female; D005809:Genes, Regulator; D008040:Genetic Linkage; D007521:Isocitrate Dehydrogenase / Q000032:analysis*; D008297:Male; D009249:NADP; D010734:Phosphogluconate Dehydrogenase / Q000032:analysis*; D011123:Polyploidy; D012730:Sex Chromosomes
chemical_list: D009249:NADP; D007521:Isocitrate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase