Separation of rubella IgM, IgA, and IgG antibodies by gel filtration on agarose.
Identification of the immunoglobulin classes of rubella antibodies is the only reliable tool for the diagnosis of a recent primary infection with rubella within 6-8 weeks of infection. Gel filtration permits the separation of IgM and IgA antibodies, both characteristic for a recent infection, from persistent rubella IgG antibody (which indicates pre-existing immunity). Gel filtration on agarose offers advantages over the other methods for separating immunoglobulin classes. Fractionation of specific rubella antibodies is important not only in all cases where a serum sample was taken too late and a rise of antibody titre missed, but also in cases with significant antibody rise to distinguish a secondary immune response after rubella reinfection.
D000914:Antibodies, Viral / Q000032:analysis*; D002847:Chromatography, Agarose; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D012409:Rubella / Q000175:diagnosis / Q000276:immunology*; D012412:Rubella virus / Q000276:immunology
D000914:Antibodies, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M