Incompatibility in Podospora anserina: comparative properties of the antagonistic cytoplasmic factors of a nonallelic system.
In the fungus Podospora anserina, the interaction between the nonallelic incompatible R and V genes has two consequences: a lytic reaction due to the synthesis of specific proteolytic enzymes, and a quenching in protein and ribonucleic acid synthesis. The incompatibility reaction when vegetative or sexual R and V cells fuse is asymmetric: it is induced only in the R protoplasm. The cessation in ribonucleic acid and protein synthesis was investigated in heterokaryotic strains carrying the antagonistic R and V genes and their "neutral" r and v alleles. Asymmetry between R and V genes lies in the fact that the strains homozygous for the R genes are the only strains that cannot grow. From these results it is postulated that the V-gene product is a diffusible cytoplasmic factor and that the R-gene product, which is nonautonomous, is a ribosomal component.
D000483:Alleles; D001203:Ascomycota / Q000502:physiology*; D002250:Carbon Radioisotopes; D003470:Culture Media; D003593:Cytoplasm / Q000502:physiology; D004096:Dihydrostreptomycin Sulfate / Q000494:pharmacology; D005306:Fertilization; D005656:Fungal Proteins / Q000378:metabolism; D005796:Genes; D005838:Genotype; D007553:Isotope Labeling; D011480:Protease Inhibitors; D012313:RNA / Q000378:metabolism; D012270:Ribosomes / Q000502:physiology; D013997:Time Factors; D013098:Xylariales / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure
D002250:Carbon Radioisotopes; D003470:Culture Media; D005656:Fungal Proteins; D011480:Protease Inhibitors; D012313:RNA; D004096:Dihydrostreptomycin Sulfate