title: Inactivation of tyrosine aminotransferase in neutral homogenates and rat liver slices.
abstract: Inactivation of tyrosine aminotransferase induced in vivo by triamcinolone was studied in a homogenate incubated at neutral pH values. The integrity and the presence of subcellular particles together with a compartment of acidic pH are necessary for inactivation of tyrosine aminotransferase. It is suggested that tyrosine aminotransferase is inactivated inside lysosomes. The system responsible for inactivation of tyrosine aminotransferase was partially purified and identified with lysosomal cathepsins B and B(1). Inactivation of tyrosine aminotransferase in liver slices is controlled by the amino acid concentration and strongly stimulated by cysteine. 3,3',5-Tri-iodo-l-thyronine reversibly and strongly decreases the rate of inactivation of tyrosine aminotransferase. The effect is not due to an increased rate of tyrosine aminotransferase synthesis.
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D002403:Cathepsins; D002845:Chromatography; D003545:Cysteine / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology; D014176:Protein Biosynthesis; D051381:Rats; D013997:Time Factors; D014221:Triamcinolone / Q000494:pharmacology*; D014284:Triiodothyronine / Q000494:pharmacology; D014316:Tritium; D014444:Tyrosine Transaminase / Q000032:analysis / Q000037:antagonists & inhibitors*
chemical_list: D000596:Amino Acids; D014284:Triiodothyronine; D014316:Tritium; D014221:Triamcinolone; D000255:Adenosine Triphosphate; D014444:Tyrosine Transaminase; D002403:Cathepsins; D003545:Cysteine