title: Measurement of intracellular iodophilic polysaccharide in two cariogenic strains of Streptococcus mutans by cytochemical and chemical methods.
abstract: Quantitative cytological and chemical methods have been developed to study the intracellular iodophilic polysaccharide (IPS) content of two strains of Streptococcus mutans. The cytological method uses a periodic acid-chlorite treatment of thin sections to increase the affinity of IPS for uranyl and lead salts. This results in the IPS appearing as individual electron-dense granules which can be counted for quantitative studies. As a basis for these quantitative studies, IPS was measured chemically by dissolving whole cells with hot KOH and quantitating spectrophotometrically the amount of iodine-polysaccharide complex formed.
mesh_terms: D001431:Bacteriological Techniques; D003470:Culture Media; D003720:Densitometry; D006003:Glycogen / Q000302:isolation & purification; D007455:Iodine; D008854:Microscopy, Electron; D010504:Periodic Acid; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D011193:Potassium Iodide; D013194:Staining and Labeling; D013291:Streptococcus / Q000032:analysis* / Q000648:ultrastructure
chemical_list: D003470:Culture Media; D011135:Polysaccharides, Bacterial; D010504:Periodic Acid; D011193:Potassium Iodide; D006003:Glycogen; D007455:Iodine