Observations with G bonding of human chromosomes. Reduction of dye concentration in Soerensen buffered solutions  is sufficient for demonstrating G bands.
D002021:Buffers; D002900:Chromosomes, Human, 1-3; D002901:Chromosomes, Human, 13-15; D002902:Chromosomes, Human, 16-18; D002903:Chromosomes, Human, 19-20; D002904:Chromosomes, Human, 21-22 and Y; D002905:Chromosomes, Human, 4-5; D002906:Chromosomes, Human, 6-12 and X; D006801:Humans; D007621:Karyotyping / Q000379:methods*; D008751:Methylene Blue; D008938:Mitosis; D012730:Sex Chromosomes; D013194:Staining and Labeling; D014357:Trypsin
D002021:Buffers; D014357:Trypsin; D008751:Methylene Blue