title: Oxidation and reduction of D-xylose by cell-free extract of Pichia quercuum.
abstract: The fermentation mechanism of the simultaneous production of D-xylonic acid and xylitol from D-xylose by Pichia quercuum was studied by using a cell-free enzyme preparation. Nicotinamide adenine dinucleotide phosphate (NADP)-dependent D-xylose dehydrogenase activity and NADP-dependent D-xylose reductase activity were detected, and the oxido-reduction reaction of D-xylose was able to couple through regeneration of NADP and NADPH to produce D-xylonic acid and xylitol.
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism; D002474:Cell-Free System; D003470:Culture Media; D003956:Dialysis; D005656:Fungal Proteins / Q000032:analysis; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D014993:Xylitol / Q000378:metabolism; D014994:Xylose / Q000378:metabolism*; D015003:Yeasts / Q000201:enzymology / Q000378:metabolism*
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D009243:NAD; D009249:NADP; D014994:Xylose; D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; D014993:Xylitol