Respiration-driven proton translocation in Escherichia coli.
Measurements were made of the stoicheiometry of respiration-driven proton translocation coupled to the oxidation of NAD(P)-linked or flavin-linked substrates in intact cells of Escherichia coli. Observed stoicheiometries (-->H(+)/O quotient; Mitchell, 1966) were approx. 4 with l-malate as substrate and approx. 2 for succinate, d-lactate and glycerol oxidation. It is concluded that the potential number of equivalent energy-conservation sites associated with the respiratory chain is 2 in aerobically grown cells of E. coli harvested during the exponential phase of growth.
D000332:Aerobiosis; D004579:Electron Transport; D004734:Energy Metabolism; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D005415:Flavins / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D008293:Malates / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D011522:Protons; D013386:Succinates / Q000378:metabolism
D005415:Flavins; D007773:Lactates; D008293:Malates; D011522:Protons; D013386:Succinates; D009243:NAD; D009249:NADP; D005990:Glycerol