Biosynthesis of gamma globulin: studies in a cell-free system.
Incorporation of C(14)-amino acids into high-molecular-weight material precipitable by trichloroacetic acid indicates that microsomal cell-free systems, derived from spleens of immunized rabbits, are active in protein synthesis. Protein was made soluble by ultrasonic irradiation of the cell-free incubation mixtures, and low-molecular-weight materials were removed by dialysis and gel filtration. Chromatography and radio-immunoelectrophoresis of this soluble protein fraction reveal C(14)-labeled protein having several characteristics of gamma globulin.
D000818:Animals; D002247:Carbon Isotopes; D002845:Chromatography; D002850:Chromatography, Gel; D003956:Dialysis; D005260:Female; D007122:Immunoelectrophoresis; D066298:In Vitro Techniques; D007930:Leucine / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism*; D008970:Molecular Weight; D011817:Rabbits; D013154:Spleen; D014465:Ultrasonics; D005719:gamma-Globulins / Q000096:biosynthesis*
D002247:Carbon Isotopes; D005719:gamma-Globulins; D007930:Leucine