Studies on transmissible gastroenteritis of swine. 3. The effect of selective inhibitors of viral replication on a cytopathogenic virus from transmissible gastroenteritis.
The effect on the plaque production of the Purdue strain of cytopathogenic virus from transmissible gastroenteritis of swine by 5-bromo-2'-deoxyuridine (BUDR), 5-iodo-2'-deoxyuridine (IUDR), actinomycin-D, puromycin, and amantadine-HCI (Symmetral) has been studied.Amantadine-HCI reduced the plaque-forming units of virus per ml by approximately 98%. Puromycin prevented almost all virus reproduction while actinomycin-D caused approximately a 22% reduction. Both IUDR and BUDR produced approximately a 20% increase in plaque-forming units of virus per ml. Swine testis cells stained with acridine orange early in the course of infection contained brick-red particles in the cytoplasm, indicative of a ribonucleic acid (RNA) type virus.
D000547:Amantadine / Q000494:pharmacology*; D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D046508:Culture Techniques; D003588:Cytopathogenic Effect, Viral; D003609:Dactinomycin / Q000494:pharmacology*; D007065:Idoxuridine / Q000494:pharmacology*; D011691:Puromycin / Q000494:pharmacology*; D012367:RNA, Viral; D013194:Staining and Labeling; D013552:Swine; D014776:Virus Cultivation; D014779:Virus Replication / Q000187:drug effects*; D014713:Viruses, Unclassified / Q000302:isolation & purification*
D012367:RNA, Viral; D003609:Dactinomycin; D011691:Puromycin; D000547:Amantadine; D001973:Bromodeoxyuridine; D007065:Idoxuridine