Lysosomes of Toxoplasma gondii and their possible relation to the host-cell penetration of toxoplasma parasites.
Lysosome-like structures of Toxoplasma gondii were observed by means of vital staining with acridine orange and by the Gomori technique. These structures were found scattered over the cytoplasm but were often located at one end of the parasite. In comparison with parasites of the inoculum used for infecting HeLa cell cultures, the toxoplasma which had penetrated the HeLa cells revealed a markedly lower percentage of parasites showing lysosomal staining. After the penetration, the number of parasites with demonstrable lysosomes increased successively and, at the time for release of newly formed parasites (at 24 hr), the majority of the parasites demonstrated lysosome-like bodies in the cytoplasm. The observations are discussed with special reference to the mechanism of host-cell penetration.
D000166:Acridines; D006367:HeLa Cells; D008247:Lysosomes; D008856:Microscopy, Fluorescence; D013194:Staining and Labeling; D014122:Toxoplasma / Q000166:cytology* / Q000502:physiology
D000166:Acridines