The partial sequence of two large peptides from the N-terminal half of heavy chains from normal rabbit immunoglobulin G.
The partial amino acid sequence of two large peptides is described. These were prepared from the N-terminal half of the heavy chain of immunoglobulin G from pooled normal rabbit serum by tryptic digestion after the in-amino groups of the lysine residues had been blocked with S-ethyl trifluorothioacetate. These peptides are believed to account for about 145 residues of fragment C-1, the N-terminal section of rabbit immunoglobulin G heavy chain prepared by cyanogen bromide cleavage. The evidence from the present paper and the preceding paper (Cebra, Givol & Porter, 1968) suggests that it may be possible to deduce a predominant amino acid sequence for most, if not all, of this section of the molecule.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002247:Carbon Isotopes; D002482:Cellulose; D002852:Chromatography, Ion Exchange; D004586:Electrophoresis; D006863:Hydrogen-Ion Concentration; D010209:Paper; D010455:Peptides / Q000032:analysis*; D011492:Protein Hydrolysates; D011817:Rabbits; D012995:Solubility; D014357:Trypsin; D005719:gamma-Globulins / Q000032:analysis*
D000596:Amino Acids; D002247:Carbon Isotopes; D010455:Peptides; D011492:Protein Hydrolysates; D005719:gamma-Globulins; D002482:Cellulose; D014357:Trypsin