Effect of the fluorescein to protein ratio on anti-Treponema pallidum conjugates.
The effect of using different labeling techniques and various amounts of fluorescein-isothiocyanate was tested with several pools of antisera to Treponema pallidum. Optimal labeling occurred in all serum pools at a fluorescien to protein ratio of 15. The use of pure dye and the dialysis method of labeling is recommended as the method of choice because of its effectiveness in the reduction of nonspecific staining.
D000818:Animals; D000937:Antigen-Antibody Reactions; D003956:Dialysis; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D008722:Methods; D011506:Proteins; D011817:Rabbits; D013194:Staining and Labeling; D013861:Thiocyanates; D013997:Time Factors; D014210:Treponema pallidum / Q000276:immunology*
D005452:Fluoresceins; D007106:Immune Sera; D011506:Proteins; D013861:Thiocyanates