Use of circumferential tangential cryostat sections of the intestine in the diagnosis of Hirschsprung's disease.
Technical and staining methods are described for making tangential sections using a specially prepared chuck surface in a cryostat. The method gives a rapid definitive diagnosis of Hirschsprung's disease in children.
D002675:Child, Preschool; D006801:Humans; D008531:Megacolon / Q000175:diagnosis* / Q000473:pathology; D008867:Microtomy; D009197:Myenteric Plexus / Q000473:pathology; D013194:Staining and Labeling