Alkaline solvent systems for thin-layer chromatography of bile acids.
Thin-layer chromatographic separation of the common bile acids and their taurine and glycine conjugates in chloroform-methanol-ammonia is reported. An alkaline system offers advantages for the separation and nondestructive staining of bile acid conjugates.
D000641:Ammonia; D001647:Bile Acids and Salts / Q000032:analysis*; D002855:Chromatography, Thin Layer; D006863:Hydrogen-Ion Concentration; D008722:Methods; D012822:Silicon Dioxide; D012997:Solvents; D013194:Staining and Labeling; D014466:Ultraviolet Rays
D001647:Bile Acids and Salts; D012997:Solvents; D012822:Silicon Dioxide; D000641:Ammonia