Colicin K. 8. The immunological properties of mitomycin-induced colicin K.
Partially purified colicin K (mCol K) has been obtained from cultures of Escherichia coli K235 induced with mitomycin C. Unlike colicin K (Col K) derived from noninduced cultures of E. coli K235, mitomycin-induced colicin K (mCol K) is not associated with the type O-specific antigen of the colicinogenic bacillus. mCol K elicits in rabbits specific antibodies which precipitate and neutralize the homologous bacteriocin. These colicin-specific antibodies are not precipitated by the colicin-O antigen complex derived from noninduced bacteria. Colicin-neutralizing antibodies can be separated by zone electrophoresis into fractions having different electrophoretic mobilities. The antibodies with lower mobility strongly precipitate the homologous antigen mCol K; those with higher mobility neutralize the bacteriocin and form soluble antigen-antibody complexes.
D000818:Animals; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D003087:Colicins; D004586:Electrophoresis; D004926:Escherichia coli / Q000187:drug effects*; D007106:Immune Sera; D005779:Immunodiffusion; D008722:Methods; D008937:Mitomycins / Q000494:pharmacology*; D009500:Neutralization Tests; D011233:Precipitin Tests; D011817:Rabbits; D005719:gamma-Globulins
D003087:Colicins; D007106:Immune Sera; D008937:Mitomycins; D005719:gamma-Globulins