Microtubules in brain homogenates.
Microtubules from neurons are preserved in homogenates of mammalian brain by medium containing organic solvents at acidic pH. By means of negative staining and electron microscopy, the relative concentration of microtubules in suspensions can be assayed. Microtubules from brain have a filamentous and substructure.
D000818:Animals; D001921:Brain / Q000166:cytology*; D002021:Buffers; D003593:Cytoplasm; D006018:Glycols; D006863:Hydrogen-Ion Concentration; D008722:Methods; D008854:Microscopy, Electron; D009474:Neurons / Q000166:cytology*; D010772:Phosphotungstic Acid; D013194:Staining and Labeling; D014021:Tissue Preservation
D002021:Buffers; D006018:Glycols; D010772:Phosphotungstic Acid