A DNA-directed cell-free system for beta-galactosidase synthesis; characterization of the de novo synthesized enzyme and some aspects of the regulation of synthesis.
D002247:Carbon Isotopes; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D002701:Chloramphenicol / Q000494:pharmacology; D003090:Coliphages; D000242:Cyclic AMP / Q000494:pharmacology; D004279:DNA, Viral / Q000494:pharmacology*; D003609:Dactinomycin / Q000494:pharmacology; D003864:Depression, Chemical; D004794:Enzyme Repression; D004926:Escherichia coli / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D005696:Galactosidases / Q000096:biosynthesis*; D005796:Genes; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008722:Methods; D009711:Nucleotides / Q000494:pharmacology; D009876:Operon; D011489:Protein Denaturation; D012293:Rifampin / Q000494:pharmacology; D013268:Stimulation, Chemical; D013816:Thermodynamics; D013997:Time Factors; D014461:Ultracentrifugation
D002247:Carbon Isotopes; D004279:DNA, Viral; D009711:Nucleotides; D003609:Dactinomycin; D002701:Chloramphenicol; D000242:Cyclic AMP; D005696:Galactosidases; D007930:Leucine; D012293:Rifampin