Mixed culture cytopathogenicity: a new test for growth of murine leukemia viruses in tissue culture.
Tissue culture cells of a Rous virus-induced rat tumor undergo syncytium formation when placed in contact with mouse embryo cell cultures infected with murine leukemia viruses. This phenomenon can be used as a cytopathic end point for isolating and titrating these viruses in tissue culture. The principle should be applicable to detection of leukemia viruses of other species.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000302:isolation & purification*; D001681:Biological Assay; D002460:Cell Line; D003470:Culture Media; D046508:Culture Techniques; D003588:Cytopathogenic Effect, Viral; D009052:Leukemia Virus, Murine / Q000302:isolation & purification*; D008722:Methods; D051379:Mice; D051381:Rats; D013194:Staining and Labeling; D014776:Virus Cultivation
D003470:Culture Media