Antigen-induced changes in lymphoid cell histones. IV. Changes in solubility of isolated chromatin.
In this study we have examined the solubility of deoxyribonucleoprotein (DNP) isolated from control and antigen-affected thymocytes. 2-M sodium chloride extracts containing the DNP of rat thymus glands were serially diluted. A comparison was made of the effect of dilution on fiber formation in the control and test series. Fiber formation is usually complete for the control material at a salt concentration between 0.63 and 0.57 M. The test material shows some fiber formation within this range. However, a significant portion of the DNP is precipitated at dilutions of 0.54-0.48 M. Ammoniacal silver (A-S) stains the control fibers a characteristic yellowish color. With the test material, those fibers formed within the control range tended to be stained yellowish brown by A-S, whereas those formed only after greater dilution stained blackish. These data, coupled with our previous observations on altered A-S staining, clearly demonstrate an antigen-induced physical and/or chemical alteration of the histone or histone-DNA complex of lymphoid cell chromatin.
D000818:Animals; D000941:Antigens / Q000494:pharmacology*; D002467:Cell Nucleus; D004247:DNA / Q000032:analysis*; D006657:Histones / Q000032:analysis; D008221:Lymphoid Tissue / Q000187:drug effects*; D051381:Rats; D012995:Solubility; D013194:Staining and Labeling; D013950:Thymus Gland
D000941:Antigens; D006657:Histones; D004247:DNA