Effect of respiratory inhibitors on the motility of Pseudomonas fluorescens.
The translational motility of Pseudomonas fluorescens was weakly inhibited by oligomycin, Dicumarol, 2,4-dinitrophenol, 2n-heptyl-4-hydroxyquinoline N-oxide, and potassium cyanide. Atabrine and antimycin A together with potassium cyanide immediately immobilized this bacterium, but antimycin A alone was without effect. Gramicidin D also immobilized P. fluorescens, but its action was inhibited by K(+) and NH(4) (+) ions. In like manner, the effect of p-chloromercuribenzoate could be counteracted with cysteine, thereby suggesting the involvement of -SH groups in flagellar motility processes. It appears that the energy required for motility of P. fluorescens is generated by oxidative phosphorylation mediated by the cytochrome system.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000968:Antimycin A / Q000494:pharmacology; D001386:Azides / Q000494:pharmacology; D002729:Chloromercuribenzoates / Q000494:pharmacology; D003486:Cyanides / Q000494:pharmacology; D001728:Dicumarol / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology; D009068:Movement / Q000187:drug effects*; D009840:Oligomycins / Q000494:pharmacology; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption / Q000187:drug effects*; D010636:Phenols / Q000494:pharmacology; D011549:Pseudomonas / Q000187:drug effects* / Q000378:metabolism; D011796:Quinacrine / Q000494:pharmacology; D011804:Quinolines / Q000494:pharmacology; D014449:Tyrothricin / Q000494:pharmacology
D001386:Azides; D002729:Chloromercuribenzoates; D003486:Cyanides; D004140:Dinitrophenols; D009840:Oligomycins; D010636:Phenols; D011804:Quinolines; D014449:Tyrothricin; D000968:Antimycin A; D001728:Dicumarol; D000255:Adenosine Triphosphate; D011796:Quinacrine