Colostral immunoglobulin-A: synthesis in vitro of T-chain by rabbit mammary gland.
When minced mammary tissue from lactating rabbits was incubated in vitro with C(14)-labeled lysine and isoleucine, it incorporated radio-activity into colostral immunoglobulin A. The only portion of this colostral molecule with significant labeling was T-chain, with little or no labeling of light or heavy chains. It was thus demonstrated that T-chains are synthesized by mammary gland. Because the remainder of the molecule was derived from unlabeled material, in vivo it was probably derived from serum.
D000818:Animals; D001345:Autoradiography; D001693:Biological Transport, Active; D002247:Carbon Isotopes; D002845:Chromatography; D003126:Colostrum / Q000032:analysis*; D005260:Female; D007122:Immunoelectrophoresis; D007532:Isoleucine / Q000378:metabolism; D007774:Lactation; D008239:Lysine / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000378:metabolism*; D011247:Pregnancy; D011817:Rabbits; D005719:gamma-Globulins / Q000096:biosynthesis*
D002247:Carbon Isotopes; D005719:gamma-Globulins; D007532:Isoleucine; D008239:Lysine