Surface localization of Escherichia coli 5'-nucleotidase by electron microscopy.
The 5'-nucleotidase of Escherichia coli was shown to be located at the cell wall surface by histochemical techniques utilizing the deposition of inorganic phosphate. Penetration of the 5'-nucleotidase in the periplasmic space was seen only in cells treated with ethylenediaminetetraacetic acid (EDTA)-tris(hydroxymethyl)aminomethane (Tris). The 3'-nucleotidase of E. coli was also found to have a surface location, and periplasmic precipitation of inorganic phosphate was seen only after EDTA-Tris-sucrose exposure.
D002473:Cell Wall / Q000201:enzymology*; D004492:Edetic Acid; D004926:Escherichia coli / Q000201:enzymology*; D006651:Histocytochemistry; D008854:Microscopy, Electron; D009708:Nucleotidases / Q000032:analysis*; D009995:Osmosis; D010406:Penicillins / Q000494:pharmacology; D013194:Staining and Labeling; D013395:Sucrose
D010406:Penicillins; D013395:Sucrose; D004492:Edetic Acid; D009708:Nucleotidases