Developments in immunofluorescence: the need for standardization.
DIFFICULTIES EXPERIENCED BY THE NEWCOMER TO THE FLUORESCENT ANTIBODY STAINING METHOD LARGELY ARISE FROM TWO CAUSES: (1) the many modifications in the procedure which have been suggested over the last decade; (2) the variability of the basic reagent, fluorescent conjugate, in terms of potency and specificity. The ensuing problem of non-reproducibility is clearly an important factor for clinical laboratories now faced with increasing demands for routine tests involving immunofluorescence. In this paper a simple approach to standardization is described which, if adopted, would lead to a more efficient use of the method.
D000937:Antigen-Antibody Reactions; D004356:Drug Storage; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique / Q000592:standards*; D005612:Freeze Drying; D006801:Humans; D007106:Immune Sera; D008856:Microscopy, Fluorescence; D013194:Staining and Labeling
D005452:Fluoresceins; D007106:Immune Sera