Cholinesterase activity of motor end plate in human skeletal muscle.
The activity and properties of cholinesterase of the motor end plate in human intercostal muscle were studied in the isolated muscle membrane. This preparation was used because cholinesterase activity of the membrane preparation was localized in the motor end plate without contamination of cholinesterase of other muscle components. Under the experimental conditions, cholinesterase in a human end plate hydrolyzed 1.21 x 10(8) molecules of acetylcholine per msec, which is smaller than hydrolysis of 2.69 x 10(8) by a motor end plate of rat intercostal muscle. Studies with cholinesterase inhibitors and specific substrates indicated that about 90% of cholinesterase of human motor endplates is acetylcholinesterase, and about 10% is pseudocholinesterase. The end plate cholinesterase had an optimal pH of 7.8 and a Michaelis-Menten constant of 4.15 mmoles/liter, and was stable at 4 degrees C for at least 4 wk. Motor end plates were estimated to contain only about 2% of the total cholinesterase activity of human intercostal muscle, compared with about 20% in rat tibialis anterior muscle. The difference is due to the lower cholinesterase activity of the motor end plate and higher cholinesterase activity of non-end plate components in human muscle than in rat muscle. The isolated muscle membrane provides a useful preparation for the study of the properties of motor end plate in human skeletal muscle.
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D001001:Anura; D002415:Cats; D002800:Cholinesterase Inhibitors; D002802:Cholinesterases / Q000378:metabolism*; D006168:Guinea Pigs; D006651:Histocytochemistry; D006801:Humans; D007366:Intercostal Muscles / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D008566:Membranes / Q000201:enzymology; D051379:Mice; D008858:Microscopy, Phase-Contrast; D009132:Muscles / Q000201:enzymology; D009469:Neuromuscular Junction / Q000201:enzymology*; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D013194:Staining and Labeling
D002800:Cholinesterase Inhibitors; D002802:Cholinesterases; D000109:Acetylcholine