Novel ribonucleic acid species accumulated in the dark in the blue-green alga Anacystis nidulans.
In the dark, the obligately photoautotrophic blue-green alga Anacystis nidulans accumulates large relative amounts of two novel stable ribonucleic acid species (RNAs). These species are also made in illuminated cells but are unstable in them. When darkened cells are reilluminated, these RNAs are rapidly degraded; degradation is inhibited by chloramphenicol. Upon denaturation with heat or urea, one novel species (0.33 x 10(6) daltons) dissociates into two fragments that comigrate with the second novel species (0.16 x 10(6) daltons) on polyacrylamide gels. Both RNAs are associated with particles sedimenting between 30S and 50S through sucrose gradients and are removed from these particles at low magnesium concentration. The function(s) of these RNAs remains unknown.
D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D002701:Chloramphenicol / Q000494:pharmacology; D000458:Cyanobacteria / Q000032:analysis / Q000378:metabolism*; D003624:Darkness; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D008027:Light; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation / Q000187:drug effects; D010761:Phosphorus Radioisotopes; D012313:RNA / Q000032:analysis / Q000096:biosynthesis*; D012335:RNA, Ribosomal / Q000032:analysis / Q000096:biosynthesis; D014316:Tritium; D014508:Urea / Q000494:pharmacology
D010761:Phosphorus Radioisotopes; D012335:RNA, Ribosomal; D014316:Tritium; D012313:RNA; D002701:Chloramphenicol; D014508:Urea