Aflatoxin-producing strains of Aspergillus flavus detected by fluorescence of agar medium under ultraviolet light.
The fluorescence method of detecting aflatoxin-producing strains of Aspergillus flavus and related species utilizes the ultraviolet-induced fluorescence of aflatoxin produced in a modified Czapek's solution agar containing corn steep liquor, HgCl(2), and (NH(4))H(2)PO(4) instead of NaNO(3). The presence of aflatoxin is confirmed by thin-layer chromatography of CHCl(3) extracts of the fluorescing agar.
D000348:Aflatoxins / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D001230:Aspergillus / Q000302:isolation & purification* / Q000378:metabolism; D001231:Aspergillus flavus / Q000302:isolation & purification / Q000378:metabolism; D003470:Culture Media; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D008860:Microscopy, Ultraviolet; D013696:Temperature; D013997:Time Factors
D000348:Aflatoxins; D003470:Culture Media