Microtiter solid-phase radioimmunoassay for hepatitis B antigen.
A micro-solid-phase radioimmunoassay (micro-SPRIA) for hepatitis B antigen (HB Ag) was developed for use with microtiter serological equipment. Radiolabeled immunoglobulin G was prepared from human and animal sera containing hepatitis B antibody (HB Ab); it was not necessary to isolate specific HB Ab by immunochemical means. A micro-SPRIA prepared with guinea pig reagents was approximately as sensitive as the AusRIA radioimmunoassay, but, like the AusRIA test, yielded false positive results. A micro-SPRIA prepared with human reagents was slightly less sensitive but did not yield false positive results. These micro-SPRIA tests offer several advantages, including conservation of reagents, adaptability to other antigen-antibody systems, ease of performance (especially when testing large numbers of specimens), and economy.
D000818:Animals; D000918:Antibody Specificity; D004355:Drug Stability; D006168:Guinea Pigs / Q000276:immunology; D000882:Haplorhini / Q000276:immunology; D006505:Hepatitis / Q000276:immunology; D006511:Hepatitis B Antigens / Q000032:analysis*; D006801:Humans; D007106:Immune Sera; D007074:Immunoglobulin G / Q000032:analysis; D007456:Iodine Isotopes; D008251:Macaca / Q000276:immunology; D008722:Methods; D011863:Radioimmunoassay; D012834:Silver
D006511:Hepatitis B Antigens; D007106:Immune Sera; D007074:Immunoglobulin G; D007456:Iodine Isotopes; D012834:Silver