Isolation of an acidic surface antigen from a conventional strain of Staphylococcus aureus.
Staphylococcus aureus strain 7007, a prototype isolated from a hospital burn unit, was shown to exhibit a significant degree of resistance to ingestion by mouse polymorphonuclear leukocytes. An acidic surface antigen was isolated from strain 7007 by a combination of 10% trichloroacetic acid extraction, ion-exchange chromatography, and gel filtration. Chemical analysis indicated that the surface antigen consists of an unknown aminouronic acid and an amino sugar. Immunochemical analysis suggested that the 7007 antigen is a common feature of all the strains collected from the burn unit. No cross-reactivity was observed between the carbohydrate preparations of various hospital staphylococcal strains and the 7007 heteropolymer. These results suggested the possibility that the surface antigen of strain 7007 represents a strain- or type-specific antigen.
D000372:Agglutination Tests; D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D002056:Burns / Q000382:microbiology; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003429:Cross Reactions; D006595:Hexosamines / Q000032:analysis; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007962:Leukocytes; D051379:Mice; D010587:Phagocytosis; D013045:Species Specificity; D013210:Staphylococcus / Q000276:immunology*; D014238:Trichloroacetic Acid; D014574:Uronic Acids / Q000032:analysis
D000942:Antigens, Bacterial; D006595:Hexosamines; D014574:Uronic Acids; D014238:Trichloroacetic Acid