Binding of (14C)penicillin G to the membrane-bound and the purified D-alanine carboxypeptidases from Bacillus stearothermophilus and Bacillus subtilis and its release.
D000409:Alanine; D001407:Bacillus / Q000201:enzymology / Q000378:metabolism*; D001412:Bacillus subtilis / Q000201:enzymology / Q000378:metabolism*; D001665:Binding Sites; D002250:Carbon Radioisotopes; D002268:Carboxypeptidases / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism; D002854:Chromatography, Paper; D006146:Guanidines; D006207:Half-Life; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010400:Penicillin G / Q000378:metabolism*; D010405:Penicillinase / Q000378:metabolism; D011402:Pronase; D011485:Protein Binding; D011955:Receptors, Drug; D012967:Sodium Dodecyl Sulfate; D013045:Species Specificity; D013696:Temperature; D013997:Time Factors; D014508:Urea
D002250:Carbon Radioisotopes; D006146:Guanidines; D011955:Receptors, Drug; D012967:Sodium Dodecyl Sulfate; D014508:Urea; D002268:Carboxypeptidases; D011402:Pronase; D010405:Penicillinase; D000409:Alanine; D010400:Penicillin G