Specificity and induction of undecyl acetate esterase from Pseudomonas cepacia grown on 2-tridecanone.
Undecyl acetate esterase from Pseudomonas cepacia grown on 2-tridecanone was strongly inhibited by organophosphates and other esterase inhibitors. Also, p-chloromercuribenzoate at 1 x 10(-4) M showed a 70% inhibition of esterase activity. The enzyme hydrolyzed both aliphatic and aromatic acetate esters at substrate concentrations of 0.25 M. Under these conditions the highest reaction rate was toward undecyl acetate. No lipase or proteolytic activity was demonstrated. Undecyl acetate esterase was classified as a carboxylesterase (B-esterase). Cell-free activity studies on the production of undecyl acetate esterase grown on different carbon sources plus zymogram studies demonstrated that the enzyme was inducible when 2-tridecanone, 2-tridecanol, undecyl acetate and, to a lesser extent, 1-undecanol were growth substrates. Induction of undecyl acetate esterase during oxidation of 2-tridecanone supports the view that undecyl acetate is an intermediate in the degradation of the methyl ketone.
D000085:Acetates / Q000378:metabolism; D000438:Alcohols / Q000378:metabolism; D001151:Arsenic / Q000494:pharmacology; D001386:Azides / Q000494:pharmacology; D002474:Cell-Free System; D002729:Chloromercuribenzoates / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction; D004950:Esterases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004952:Esters / Q000378:metabolism; D005459:Fluorides / Q000494:pharmacology; D006868:Hydrolysis; D007461:Iodoacetates / Q000494:pharmacology; D007659:Ketones / Q000378:metabolism*; D009942:Organometallic Compounds / Q000494:pharmacology; D009943:Organophosphorus Compounds / Q000494:pharmacology; D010830:Physostigmine / Q000494:pharmacology; D011549:Pseudomonas / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism
D000085:Acetates; D000438:Alcohols; D001386:Azides; D002729:Chloromercuribenzoates; D004952:Esters; D007461:Iodoacetates; D007659:Ketones; D009942:Organometallic Compounds; D009943:Organophosphorus Compounds; D004492:Edetic Acid; D010830:Physostigmine; D004950:Esterases; D001151:Arsenic; D005459:Fluorides