Simplified method for producing pyocins from Pseudomonas aeruginosa.
Good yields of pyocin are obtained when Pseudomonas aeruginosa is grown in Trypticase soy broth (without glucose) (BBL) plus 1% potassium nitrate. As a result, pyocin production for routine epidemiological typing can be done in screw-cap tubes, without mechanical agitation or mitomycin C induction.
D001430:Bacteriocins / Q000032:analysis / Q000096:biosynthesis*; D001431:Bacteriological Techniques; D003470:Culture Media; D008722:Methods; D009566:Nitrates / Q000378:metabolism; D009573:Nitrites / Q000096:biosynthesis; D011188:Potassium; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000254:growth & development / Q000378:metabolism*
D001430:Bacteriocins; D003470:Culture Media; D009566:Nitrates; D009573:Nitrites; D011188:Potassium