Use of ultraviolet radiation to locate dipicolinic acid in Bacillus cereus spores.
A novel method is described that allows a direct determination of the location of a portion of the dipicolinic acid (DPA) in spores. Ultraviolet (UV) irradiation is shown to cause cross-linkage of DPA to spore proteins which have the characteristics of membrane proteins. We suggest that DPA resides in the inner forespore membrane (IFSM) and spore cytoplasm (i.e., the spore protoplast). Only that portion of the DPA in the vicinity of the IFSM appeared to form UV-induced DPA-protein adducts.
D001409:Bacillus cereus / Q000032:analysis* / Q000378:metabolism / Q000528:radiation effects; D001426:Bacterial Proteins / Q000378:metabolism; D002250:Carbon Radioisotopes; D002458:Cell Fractionation; D002462:Cell Membrane / Q000032:analysis; D002499:Centrifugation, Density Gradient; D003593:Cytoplasm / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D008722:Methods; D010848:Picolinic Acids / Q000302:isolation & purification* / Q000378:metabolism; D011485:Protein Binding / Q000528:radiation effects; D011830:Radiation Effects; D013171:Spores, Bacterial / Q000032:analysis* / Q000378:metabolism / Q000528:radiation effects; D014316:Tritium; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D002250:Carbon Radioisotopes; D010848:Picolinic Acids; D014316:Tritium