The purification and properties of extracellular glycosidases of the cellular slime mould Dictyostelium discoideum.
The purification of beta-N-acetylhexosaminidase, alpha-glucosidase, alpha-mannosidase and beta-glucosidase from the spent growth medium of Dictyostelium discoideum strain Ax-2 myxamoebae is described. beta-N-Acetylhexosaminidase and alpha-glucosidase were obtained in high yield and as homogeneous preparations whereas the alpha-mannosidase preparation consisted of two electrophoretically distinct isoenzymes. The physical, chemical and kinetic properties of these enzymes are described.
D000081:Acetamides; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003208:Concanavalin A; D004355:Drug Stability; D004591:Electrophoresis, Polyacrylamide Gel; D004592:Electrophoresis, Starch Gel; D005959:Glucosidases / Q000302:isolation & purification / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D006596:Hexosaminidases / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D005779:Immunodiffusion; D007527:Isoenzymes; D007700:Kinetics; D008358:Mannose; D008970:Molecular Weight; D009235:Myxomycetes / Q000201:enzymology*; D009596:Nitrophenols; D013053:Spectrophotometry; D013696:Temperature; D013997:Time Factors
D000081:Acetamides; D007527:Isoenzymes; D009596:Nitrophenols; D003208:Concanavalin A; D005959:Glucosidases; D006026:Glycoside Hydrolases; D006596:Hexosaminidases; D008358:Mannose