Isolation of DNA-membrane complex in Bacillus subtilis.
A DNA-membrane complex of Bacillus subtilis was separated from the bulk of the membrane by CsCl-sucrose double gradient centrifugation. The complex has a CsCl-sucrose buoyant density of 1.35-1.45 g/cm(3). Gel electrophoretic analyses show that the complex has about 5% of the whole membrane proteins and contains proteins unique to the complex as well as a small amount of membrane proteins. DNA in the complex is enriched for a genetic marker close to the origin (pur A16) and for one near the terminus (metB5). Artifactual formation of the complex during the lysate preparation was shown to be unlikely.
D001412:Bacillus subtilis / Q000648:ultrastructure*; D001426:Bacterial Proteins / Q000032:analysis; D002250:Carbon Radioisotopes; D002458:Cell Fractionation; D002462:Cell Membrane / Q000032:analysis; D002499:Centrifugation, Density Gradient; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D007930:Leucine / Q000378:metabolism; D013941:Thymine / Q000378:metabolism; D014316:Tritium
D001426:Bacterial Proteins; D002250:Carbon Radioisotopes; D004269:DNA, Bacterial; D014316:Tritium; D007930:Leucine; D013941:Thymine