The regulation of transport of glucose and methyl alpha-glucoside in Pseudomonas aeruginosa.
The methyl alpha-glucoside-transport system of Pseudomonas aeruginosa has been characterized with respect to its specificity, energy-dependence, kinetics and regulation. The uptake of glucose involved two components, one of which transported glucose (K(m)=8mum) and methyl alpha-glucoside (K(m)=2.8mm) whereas the other was more complex, involving the extracellular activity of glucose dehydrogenase. Mutants defective in this enzyme have been isolated and characterized. The methyl alpha-glucoside-glucose-transport system was repressed when the organism was grown in the absence of glucose; the induction of this transport system by glucose, and its activity once induced, were inhibited by products of citrate metabolism.
D001386:Azides / Q000494:pharmacology; D001692:Biological Transport; D001693:Biological Transport, Active; D002247:Carbon Isotopes; D002462:Cell Membrane / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D002854:Chromatography, Paper; D004790:Enzyme Induction; D004794:Enzyme Repression; D004926:Escherichia coli / Q000378:metabolism; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D005949:Glucose Oxidase / Q000378:metabolism; D006027:Glycosides / Q000378:metabolism*; D007700:Kinetics; D000432:Methanol / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D013045:Species Specificity; D013997:Time Factors
D001386:Azides; D002247:Carbon Isotopes; D006027:Glycosides; D005949:Glucose Oxidase; D005947:Glucose; D000432:Methanol