New sensitive assay for phosphatidylserine decarboxylase based on the detection of CO2 from nonradiolabeled phosphatidylserine.
A new rapid assay for phosphatidylserine decarboxylase, which is sensitive in the nanomolar range, is described. Synthesis of radiolabeled phosphatidylserine for use as a substrate is not required, since the assay, unlike previous ones, is based on the detection of CO(2) liberated from unlabeled phosphatidylserine. The assay employs a gas chromatographic procedure for the analysis of methane formed by catalytic conversion of the CO(2) produced as a product of the enzymatic reaction.
D000818:Animals; D002245:Carbon Dioxide / Q000032:analysis*; D002247:Carbon Isotopes; D002262:Carboxy-Lyases / Q000032:analysis* / Q000378:metabolism; D002848:Chromatography, DEAE-Cellulose; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D005069:Evaluation Studies as Topic; D007700:Kinetics; D008722:Methods; D010714:Phosphatidylethanolamines; D013054:Spectrophotometry, Atomic; D013769:Tetrahymena pyriformis / Q000201:enzymology
D002247:Carbon Isotopes; D010714:Phosphatidylethanolamines; D002245:Carbon Dioxide; D002262:Carboxy-Lyases