Mutants of Bacillus subtilis bacteriophage phi e defective in dTTP-dUTP nucleotidohydrolase.
Mutants of bacteriophage phie inducing only 1 to 5% of wild-type levels of dTTP-dUTP nucleotidohydrolase give normal bursts of viable progeny phage whose DNA contains 5 to 10% thymine (but no uracil) in place of 5-hydroxymethyluracil. The relative heat lability of one phage mutant enzyme solubilized from the membrane fraction of infected cells suggests that a phie gene codes for the induced dTTPase-dUTPase.
D001412:Bacillus subtilis; D001435:Bacteriophages / Q000201:enzymology* / Q000254:growth & development; D004279:DNA, Viral / Q000032:analysis; D003673:Defective Viruses / Q000201:enzymology*; D009154:Mutation; D011755:Pyrophosphatases / Q000378:metabolism*; D013696:Temperature; D013941:Thymine / Q000032:analysis; D013942:Thymine Nucleotides; D014498:Uracil / Q000032:analysis; D014500:Uracil Nucleotides; D014779:Virus Replication
D004279:DNA, Viral; D013942:Thymine Nucleotides; D014500:Uracil Nucleotides; D014498:Uracil; D011755:Pyrophosphatases; D013941:Thymine