Lipopolysaccharide containing L-acofriose in the filamentous blue-green alga Anabaena variabilis.
For the first time, an O-antigenic lipopolysaccharide (LPS) has been isolated from a filamentous blue-green alga (Anabaena variabilis). It was extractable with phenol-water, resulting in extraction of the bulk of the LPS into the phenol phase. The polysaccharide moiety of this LPS consists of l-rhamnose, its 3-O-methyl ether l-acofriose, d-mannose, d-glucose, and d-galactose. l-Glycero-d-mannoheptose and 2-keto-3-deoxyoctonate, the two characteristic sugar components of enteric LPS, and phosphate groups are absent from the A. variabilis O antigen. The only amino sugar present is d-glucosamine. Three hydroxy fatty acids were identified, namely, beta-hydroxymyristic, beta-hydroxypalmitic and beta-hydroxystearic acids, in addition to palmitic and unidentified fatty acid. The LPS of A. variabilis is localized in the outermost cell wall layer and behaves like a bacterial O antigen in serological tests. The passive hemagglutination yielded high titers with isolated LPS (pretreated by heat or by alkali) and rabbit antisera prepared against living or heat-killed cells. The position of the precipitation arcs after immunoelectrophoresis of the O antigen indicates the lack of charged groups. The water phase of the phenol-water extract contains, in high yield, a glucose polymer. It is serologically inactive as shown by the passive hemagglutination test and by agar-gel precipitation.
D000596:Amino Acids / Q000032:analysis; D000606:Amino Sugars / Q000032:analysis; D000681:Amylases; D000818:Animals; D000941:Antigens; D002854:Chromatography, Paper; D000458:Cyanobacteria / Q000276:immunology / Q000378:metabolism*; D003837:Deoxy Sugars / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D006003:Glycogen / Q000302:isolation & purification; D006868:Hydrolysis; D006898:Hydroxylamines / Q000032:analysis; D008070:Lipopolysaccharides / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D008358:Mannose / Q000031:analogs & derivatives / Q000032:analysis; D008970:Molecular Weight; D010636:Phenols; D010758:Phosphorus / Q000032:analysis; D011817:Rabbits / Q000276:immunology
D000596:Amino Acids; D000606:Amino Sugars; D000941:Antigens; D003837:Deoxy Sugars; D005227:Fatty Acids; D006898:Hydroxylamines; D008070:Lipopolysaccharides; D010636:Phenols; D010758:Phosphorus; C006396:acofriose; D006003:Glycogen; D000681:Amylases; D008358:Mannose