Demonstration of rubella IgM antibody by indirect fluorescent antibody staining, sucrose density gradient centrifugation and mercaptoethanol reduction.
D000914:Antibodies, Viral / Q000302:isolation & purification*; D002499:Centrifugation, Density Gradient; D003168:Complement Fixation Tests; D003937:Diagnosis, Differential; D005455:Fluorescent Antibody Technique; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000302:isolation & purification*; D008623:Mercaptoethanol; D008722:Methods; D010084:Oxidation-Reduction; D012409:Rubella / Q000151:congenital / Q000175:diagnosis / Q000276:immunology*; D012412:Rubella virus / Q000276:immunology; D013395:Sucrose; D013696:Temperature; D013997:Time Factors
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D013395:Sucrose; D008623:Mercaptoethanol