Rabbit beta-glucuronidase. Subcellular distribution and immunochemical properties.
1. The subcellular distribution of beta-glucuronidase and other hydrolases in rabbit liver was investigated. beta-Glucuronidase was found in both microsomal and lysosomal fractions. 2. Multiple forms of beta-glucuronidase were present in extracts of microsomal and lysosomal fractions. All forms were common to both fractions. 3. A specific antiserum against beta-glucuronidase was raised, and characterized by immunoprecipitation and affinity-chromatography procedures. 4. The immunological identity of the multiple forms in the pure beta-glucuronidase preparation, and the immunological identity of the beta-glucuronidase complement of lysosomal extracts with that of microsomal extracts, were demonstrated by means of the antiserum. The presence of inactive enzyme in various enzyme preparations was shown.
D000818:Animals; D002458:Cell Fractionation; D002498:Centrifugation; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D004586:Electrophoresis; D005966:Glucuronidase / Q000032:analysis* / Q000276:immunology; D006168:Guinea Pigs / Q000276:immunology; D007106:Immune Sera; D007120:Immunochemistry; D005779:Immunodiffusion; D007074:Immunoglobulin G; D007158:Immunologic Techniques; D007457:Iodine Radioisotopes; D007527:Isoenzymes; D008099:Liver / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology*; D008856:Microscopy, Fluorescence; D008862:Microsomes, Liver / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000201:enzymology; D011817:Rabbits; D013347:Subcellular Fractions / Q000201:enzymology; D013552:Swine / Q000276:immunology; D014461:Ultracentrifugation
D007106:Immune Sera; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D007527:Isoenzymes; D005966:Glucuronidase