Bacterial calcium transport: energy-dependent calcium uptake by membrane vesicles from Bacillus megaterium.
Membrane vesicles capable of energy-dependent calcium uptake have been prepared from Bacillus megaterium cells in log-phase growth or when undergoing sporulation. The uptake is dependent on the calcium concentration and appears saturable in vesicles from cells in log-phase growth. Both ascorbate and phenazine methosulfate are needed as a source of electrons for the energy-dependent increase in calcium uptake. Addition of 8 mM sodium cyanide inhibited the energy-dependent uptake. If this calcium uptake mechanism is a component of the sporulation-specific calcium accumulation process, the latter's functional expression would appear to be inhibited during log-phase growth.
D001205:Ascorbic Acid / Q000378:metabolism; D001410:Bacillus megaterium / Q000254:growth & development / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes; D002250:Carbon Radioisotopes; D002462:Cell Membrane / Q000378:metabolism*; D003486:Cyanides / Q000494:pharmacology; D004579:Electron Transport; D005971:Glutamates / Q000378:metabolism; D010619:Phenazines / Q000378:metabolism; D010848:Picolinic Acids / Q000494:pharmacology; D013171:Spores, Bacterial / Q000254:growth & development / Q000378:metabolism; D013431:Sulfates; D013997:Time Factors
D002132:Calcium Radioisotopes; D002250:Carbon Radioisotopes; D003486:Cyanides; D005971:Glutamates; D010619:Phenazines; D010848:Picolinic Acids; D013431:Sulfates; D001205:Ascorbic Acid; D002118:Calcium