Synchronous cultures of Bacillus subtilis obtained by filtration with glass fiber filters.
A simple method of potentially wide applicability for obtaining synchronous cultures of Bacillus subtilis based on size selection is described. Using glass fiber filters, a population (about 1 to 2% of the parent population) can be obtained substantially enriched for small cells which grow synchronously. A method for rapidly concentrating dilute suspensions of cells is described.
D001412:Bacillus subtilis / Q000254:growth & development*; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D003470:Culture Media; D004269:DNA, Bacterial / Q000096:biosynthesis; D005374:Filtration; D005898:Glass; D013941:Thymine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium; D014364:Tryptophan / Q000378:metabolism
D002250:Carbon Radioisotopes; D003470:Culture Media; D004269:DNA, Bacterial; D014316:Tritium; D014364:Tryptophan; D013941:Thymine