Synthesis of immunoglobulin heavy and light chains by the free ribosomes of a mouse plasma cell tumor.
Free and membrane-bound ribosomes have been isolated from a mouse plasma cell tumor secreting immunoglobulins, and allowed to synthesize proteins under cell-free conditions. Analysis of the products of synthesis by autoradiography of immunoelectrophoresis and peptide maps indicates that both types of ribosomes synthesize heavy and light immunoglobulin chains.
D000818:Animals; D001345:Autoradiography; D002247:Carbon Isotopes; D002462:Cell Membrane; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D007122:Immunoelectrophoresis; D051379:Mice; D008854:Microscopy, Electron; D010455:Peptides / Q000032:analysis; D010954:Plasmacytoma / Q000378:metabolism*; D012313:RNA / Q000032:analysis; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D005719:gamma-Globulins / Q000096:biosynthesis*
D002247:Carbon Isotopes; D010455:Peptides; D005719:gamma-Globulins; D012313:RNA