Biosynthesis of immunoglobulin A (IgA). Secretion and addition of carbohydrate to monomer and polymer forms of a mouse myeloma protein.
Cell suspensions of mouse plasma-cell tumour MOPC 315 secreting predominantly IgA (immunoglobulin A) monomer and dimer were incubated with radioactive leucine, mannose, galactose and fucose for various periods of time. The amounts of secreted and intracellular immunoglobulins were measured by co-precipitation with specific antibody, and the molecular species present were assessed by electrophoresis in polyacrylamide gels. Analysis of the secreted myeloma protein demonstrated that monomer and dimer IgA molecules are identical with respect to carbohydrate composition and rate of secretion. Within the cell, the myeloma protein is almost entirely accounted for by monomer units which either leave the cell as such or are polymerized with the addition of J chain close to the time of secretion. The results support the concept of a stepwise addition of carbohydrate residues to IgA immunoglobulin during the process of secretion. Similar patterns of carbohydrate assembly were found for the monomer or dimer molecules. Mannose residues are added at an early stage, whereas fucose is added close to the time of secretion. Galactose is also added early, but some may also be incorporated at a later stage. Control of IgA polymerization is considered unlikely to reflect regulation at the level of carbohydrate addition, and it is suggested that the critical controlling factor is the J chain.
D000818:Animals; D000906:Antibodies; D050260:Carbohydrate Metabolism; D011232:Chemical Precipitation; D004591:Electrophoresis, Polyacrylamide Gel; D005643:Fucose / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D007070:Immunoglobulin A / Q000096:biosynthesis*; D007144:Immunoglobulin J-Chains / Q000378:metabolism; D066298:In Vitro Techniques; D007930:Leucine / Q000378:metabolism; D046911:Macromolecular Substances; D008358:Mannose / Q000378:metabolism; D051379:Mice; D009194:Myeloma Proteins / Q000378:metabolism*; D009374:Neoplasms, Experimental / Q000378:metabolism; D010954:Plasmacytoma / Q000378:metabolism; D014316:Tritium
D000906:Antibodies; D007070:Immunoglobulin A; D007144:Immunoglobulin J-Chains; D046911:Macromolecular Substances; D009194:Myeloma Proteins; D014316:Tritium; D005643:Fucose; D007930:Leucine; D008358:Mannose; D005690:Galactose