The effect of ambient temperature on the anti-D assay using the Auto-Analyzer.
Using the AutoAnalyzer, the percentage agglutination effected by the anti-D antisera studied showed a varied dependence on the ambient temperature over the manifold subsequent to the incubation period at 37 degrees C. This leads to assays which are a function of the ambient temperature. It is suggested that the entry of a relatively large volume of rouleaux-dispersing agent results in an elution of bound antibody to a new position of equilibrium, the shift being dependent on the particular equilibrium constant of the antibody and the rate of its attainment on the ambient temperature. A constant ambient temperature will lead to greater accuracy of anti-D assay.
D001322:Autoanalysis; D001666:Binding Sites, Antibody; D006384:Hemagglutination; D006801:Humans; D007106:Immune Sera; D007518:Isoantibodies / Q000032:analysis*; D008297:Male; D008722:Methods; D012204:Rh-Hr Blood-Group System; D013696:Temperature
D007106:Immune Sera; D007518:Isoantibodies; D012204:Rh-Hr Blood-Group System