Semiautomated turbidimetric microbiological assay for determination of cefazolin.
The Autoturb System, a semiautomated system for photometric bioassay, was used to determine cefazolin content. Suitable conditions for the assay using Streptococcus faecalis ATCC 10541 as the indicator organism included a medium pH of 6.0 to 7.0 and an incubation time of 3 to 3.5 h at 36 C. Multiple independent assays of samples from a common batch showed the test to be highly reproducible. Accuracy of the turbidimetric assay was evaluated by comparing data obtained from a chemical (hydroxylamine) and a biological (disk diffusion) assay. The available data show the turbidimetric assay to be a rapid, accurate, and reproducible method for determining the biological activity of cefazolin samples.
D001322:Autoanalysis; D001412:Bacillus subtilis; D001681:Biological Assay; D002511:Cephalosporins / Q000032:analysis*; D003720:Densitometry; D013293:Enterococcus faecalis; D008722:Methods; D013440:Sulfides / Q000032:analysis; D013777:Tetrazoles / Q000032:analysis; D013830:Thiadiazoles / Q000032:analysis
D002511:Cephalosporins; D013440:Sulfides; D013777:Tetrazoles; D013830:Thiadiazoles