Biological, proton-magnetic-resonance- and ultraviolet-spectroscopic evidence for a molecular complex of actinomycin D and 10,11-dihydro-3H-naphth (1,2-g)indazol-7-ol.
A proton-magnetic-resonance study of the complex formed between actinomycin D and 10,11-dihydro-3H-naphth[1,2-g]indazol-7-ol strongly suggests that the indazole lies above the phenoxazine ring of actinomycin D. The complex can be destroyed by addition of dimethyl sulphoxide or dimethylformamide. The actinomycin D-indazole complex inhibits growth of Pseudomonas fluorescens and raises the thermal denaturation response of DNA. These data support the hypothesis that a molecular complex is formed which readily inhibits cell growth and interacts with DNA.
D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D006358:Hot Temperature; D007191:Indazoles; D008968:Molecular Conformation; D009281:Naphthalenes; D009691:Nucleic Acid Denaturation; D011522:Protons; D011551:Pseudomonas fluorescens / Q000187:drug effects; D011720:Pyrazoles; D013057:Spectrum Analysis
D007191:Indazoles; D009281:Naphthalenes; D011522:Protons; D011720:Pyrazoles; D003609:Dactinomycin; D004247:DNA