Time course of purine nucleoside phosphorylase occurrence in sporulation of Bacillus cereus.
Purine nucleoside phosphorylase (EC 2.4.2.1) from Bacillus cereus T was examined at hourly intervals during growth and sporulation. The enzyme has maximal activity in extracts prepared from cells during stages I and II. The activity during exponential growth is only 6.6% of the maximum and that in free spores is only 3.3%. Conservation of the purine nucleoside phosphorylase during sporulation is apparent as shown by the gradual increase in heat resistance.
D001409:Bacillus cereus / Q000201:enzymology* / Q000254:growth & development; D002474:Cell-Free System; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D010430:Pentosyltransferases / Q000096:biosynthesis* / Q000378:metabolism; D010848:Picolinic Acids / Q000096:biosynthesis; D011684:Purine Nucleosides; D013053:Spectrophotometry; D013171:Spores, Bacterial / Q000201:enzymology* / Q000254:growth & development; D013997:Time Factors
D010848:Picolinic Acids; D011684:Purine Nucleosides; D010430:Pentosyltransferases