Rabies group-specific ribonucleoprotein antigen and a test system for grouping and typing of rhabdoviruses.
Cell-associated ribonucleoprotein (RNP) was isolated from BHK-21 cells infected with several strains of rabies and rabies-related viruses. The RNP-antigen from rabies and related viruses induced the formation of complement-fixing, precipitating, and immunofluorescent antibodies, and proved to be the group-specific antigen common to all rabies viruses. Antigens of the envelope which induce virus-neutralizing antibodies are apparently determinative for the serotype of a virus as evidenced by two-way neutralization tests. A combination of these methods seems to be a useful approach to the serological grouping and typing of rhabdoviruses.
D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D003168:Complement Fixation Tests; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007668:Kidney; D008722:Methods; D051379:Mice; D008854:Microscopy, Electron; D009500:Neutralization Tests; D009698:Nucleoproteins / Q000302:isolation & purification; D012328:RNA Viruses / Q000145:classification*; D011820:Rabies virus / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity; D012703:Serotyping
D000956:Antigens, Viral; D009698:Nucleoproteins