An electrophoretic difference between surface and secreted IgM of murine splenocytes.
micro-chains on the surface of murine splenocytes are more heterogeneous on SDS-polyacrylamide gel electrophoresis than both secreted and intracellular micro-chains labeled with [(3)H]tyrosine. No difference in heterogeneity among cell surface, secreted, or intracellular L chains was detected. The possible role of carbohydrate in micro-chain heterogeneity is discussed.
D000818:Animals; D002462:Cell Membrane / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D007075:Immunoglobulin M / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology* / Q000276:immunology; D013936:Thymidine; D014316:Tritium; D014443:Tyrosine
D007075:Immunoglobulin M; D014316:Tritium; D014443:Tyrosine; D013936:Thymidine