Chondroitin sulfate: inhibition of synthesis by puromycin.
Puromycin reversibly inhibits synthesis of chondroitin sulfate by vertebral chondrocytes from 10-day-old chick embryos. Treatment of these cells with puromycin for 5 hours does not affect viability or capacity to multiply on subsequent release from their matrix. It is suggested that synthesis of this polysaccharide is coupled with that of protein; there may be a feedback control in its synthesis.
D000818:Animals; D002247:Carbon Isotopes; D002356:Cartilage / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D002642:Chick Embryo / Q000378:metabolism; D005246:Feedback; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D066298:In Vitro Techniques; D014176:Protein Biosynthesis; D011691:Puromycin / Q000494:pharmacology*; D013460:Sulfur Isotopes
D002247:Carbon Isotopes; D006025:Glycosaminoglycans; D013460:Sulfur Isotopes; D011691:Puromycin