Degradation of coxsackievirus type A9 by proteolytic enzymes.
The means by which coxsackievirus type A9 (CA9) is inactivated by proteolytic enzymes was investigated. After reaction of (14)C-leucine-labeled CA9 with Pronase, free leucine was liberated as measured by radiochromatography. Treatment of (14)C-leucine-labeled CA9 with trypsin or proteolytic filtrates of Pseudomonas aeruginosa caused the release of a variety of labeled substances. The extent of viral ribonucleic acid (RNA) release after exposure of CA9 to Pronase was determined by RNA infectivity tests or trichloroacetic acid solubility tests. Infective viral RNA was found not to be consistently released by reaction of CA9 with Pronase, but further treatment with 1% sodium dodecyl sulfate at pH 7.0 promoted viral RNA release. Sodium dodecyl sulfate treatment of CA9 that had not been reacted with Pronase did not inactivate virus or cause viral RNA release. Reaction of Pronase with (32)P-labeled CA9 resulted in the liberation of virus components soluble in cold trichloroacetic acid, whereas untreated CA9 or CA9 reacted with ribonuclease were precipitated by cold trichloroacetic acid. These results demonstrate that the primary means by which protease-sensitive enteroviruses are inactivated is by degradation of the virus capsid, with subsequent release of viral RNA.
D000818:Animals; D002250:Carbon Radioisotopes; D002854:Chromatography, Paper; D046508:Culture Techniques; D004770:Enterovirus / Q000187:drug effects / Q000276:immunology*; D000882:Haplorhini; D007668:Kidney; D007930:Leucine / Q000378:metabolism; D010447:Peptide Hydrolases / Q000494:pharmacology*; D010761:Phosphorus Radioisotopes; D011402:Pronase / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000201:enzymology; D012367:RNA, Viral; D012260:Ribonucleases / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate; D012995:Solubility; D014238:Trichloroacetic Acid; D014357:Trypsin / Q000494:pharmacology; D010948:Viral Plaque Assay; D014776:Virus Cultivation
D002250:Carbon Radioisotopes; D010761:Phosphorus Radioisotopes; D012367:RNA, Viral; D012967:Sodium Dodecyl Sulfate; D014238:Trichloroacetic Acid; D012260:Ribonucleases; D010447:Peptide Hydrolases; D014357:Trypsin; D011402:Pronase; D007930:Leucine