Specificity of formation of complexes between coat protein and bacteriophage f2 RNA.
The specificity of formation of phage f2 RNA-protein complexes was studied. Complex I contains up to 8 mol of coat protein per 1 mol of RNA. Its formation proceeds equally well in medium (i) without magnesium ions, (ii) containing magnesium ions, (iii) containing 4 mM EDTA, and (iv) at temperatures from 0 to 45 C. Complex II contains up to 200 mol of coat protein per 1 mol of RNA. Its formation is inhibited by the presence of magnesium ions in medium. Formaldehyde- or methoxyamine-treated f2 RNA in which only exposed bases were modified showed a normal pattern of complex II formation, whereas formation of complex I was inhibited or abolished. We conclude that complex I formation involves the interaction between coat protein and specific region of exposed bases in RNA. A possible site of attachment of coat protein is discussed.
D001665:Binding Sites; D002250:Carbon Radioisotopes; D003090:Coliphages / Q000378:metabolism*; D004492:Edetic Acid / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D008729:Methoxamine / Q000494:pharmacology; D009698:Nucleoproteins / Q000096:biosynthesis*; D012367:RNA, Viral / Q000378:metabolism*; D012694:Serine / Q000378:metabolism; D013696:Temperature; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis / Q000378:metabolism*
D002250:Carbon Radioisotopes; D009698:Nucleoproteins; D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium; D012694:Serine; D004492:Edetic Acid; D008729:Methoxamine; D008274:Magnesium; D014529:Uridine