Polymerization of proteins with glutaraldehyde. Soluble molecular-weight markers.
Glutaraldehyde is well known for its ability to react with proteins and to produce insoluble cross-linked aggregates. In contrast with this situation, conditions are described here which yield covalently linked soluble protein oligomers. The procedure is applicable to a wide range of proteins, and by slight variation in the reaction conditions, soluble polymers in the molecular weight range 3x10(4)-2x10(7) were produced. The products are valuable as molecular-weight markers, e.g. in sodium dodecyl sulphate-polyacrylamide-gel electrophoresis. The inherent similarities of these oligomers make them superior to commercial molecular-weight protein markers, which may have marked differences in composition and charge.
D000447:Aldehydes; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005976:Glutaral; D046911:Macromolecular Substances; D008970:Molecular Weight; D011506:Proteins; D012710:Serum Albumin, Bovine / Q000032:analysis; D012967:Sodium Dodecyl Sulfate; D012995:Solubility
D000447:Aldehydes; D000596:Amino Acids; D046911:Macromolecular Substances; D011506:Proteins; D012710:Serum Albumin, Bovine; D012967:Sodium Dodecyl Sulfate; D005976:Glutaral