Early detection of influenza virus in cell culture by means of immunofluorescence.
An indirect fluorescent antibody technique using human serum for the rapid detection of influenza A virus in monkey kidney cell cultures is described. Influenza A2 virus was isolated from 38 of 88 nose/throat swabs received from patients with suspected influenza during the winter of 1971-72.Thirty-two strains isolated in monkey kidney cell cultures were identified by immunofluorescence on the day haemadsorption was observed, 22 of them within a week of receipt of the specimen. Human antiserum was found to be very satisfactory for this purpose.
D000818:Animals; D002478:Cells, Cultured / Q000382:microbiology*; D005455:Fluorescent Antibody Technique; D000882:Haplorhini; D006383:Hemadsorption Inhibition Tests; D006386:Hemagglutination Tests; D006801:Humans; D007251:Influenza, Human / Q000175:diagnosis; D007668:Kidney; D009975:Orthomyxoviridae / Q000302:isolation & purification*; D014776:Virus Cultivation