The localization of adenosine triphosphatases in morphologically characterized subcellular fractions of guinea-pig brain.
1. The distribution of adenosine triphosphatase was studied in morphologically characterized subcellular fractions of guinea-pig brain. The conditions of homogenization were selected so as to favour the survival of nerve endings as organized structures. 2. A fraction consisting mainly of the external membranes of nerve endings was rich in a ouabain-sensitive Na(+)-K(+)-stimulated adenosine triphosphatase which closely resembled that present in the classical microsomal fraction studied by other workers, but which showed a higher specific activity. 3. A dinitrophenol-stimulated adenosine triphosphatase was located in the nerve-ending mitochondria. 4. The synaptic-vesicle fraction contained a small amount of adenosine triphosphatase that differed in its response to several ions and other compounds from the membrane, myelin and mitochondrial fractions, indicating freedom from contamination by these elements.
D000109:Acetylcholine; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology; D002118:Calcium; D002614:Chelating Agents; D002623:Chemistry Techniques, Analytical; D004140:Dinitrophenols; D004798:Enzymes; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007477:Ions; D008274:Magnesium; D008854:Microscopy, Electron; D008861:Microsomes / Q000201:enzymology*; D009638:Norepinephrine; D010042:Ouabain; D011188:Potassium / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology; D013347:Subcellular Fractions; D014465:Ultrasonics
D002614:Chelating Agents; D004140:Dinitrophenols; D004798:Enzymes; D007477:Ions; D010042:Ouabain; D012964:Sodium; D000251:Adenosine Triphosphatases; D008274:Magnesium; D000109:Acetylcholine; D011188:Potassium; D002118:Calcium; D009638:Norepinephrine