Assay of rifampicin in serum.
Two methods for the assay of rifampicin in serum are described. The first is a conventional plate diffusion method, measuring concentrations down to 0.02 mug/ml, and the second a chemical extraction followed by measurement of the inhibition of uptake of (14)C-uridine by Staphylococcus aureus, which estimates in the range of 0.02 to 0.001 mug/ml. The methods were used to measure serum concentrations in man following doses of about 1050 mg and 75 mg rifampicin.
D000362:Agar; D001681:Biological Assay / Q000379:methods; D002250:Carbon Radioisotopes; D004058:Diffusion; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D012293:Rifampin / Q000097:blood* / Q000302:isolation & purification / Q000494:pharmacology; D013210:Staphylococcus / Q000187:drug effects / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D002250:Carbon Radioisotopes; D000362:Agar; D012293:Rifampin; D014529:Uridine