Genetics and biochemistry of pyrimidine biosynthesis in Bacillus subtilis: linkage between mutations resulting in a requirement for uracil.
A number of independently derived uracil-requiring mutant strains of the parent strain 168 were mapped by inter-mutant transformation crosses. Only a few of these mutant strains were found to be transformable. Studies were performed in which these transformable uracil-requiring mutant strains were used as the recipient of deoxyribonucleic acid extracted from phenotypically similar mutant strains. The results yielded data resulting in a fine-structure map in which all mutations were found to be linked in a small region corresponding to that previously published as the uracil region of the Bacillus subtilis genome.
D001412:Bacillus subtilis / Q000378:metabolism*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003470:Culture Media; D004269:DNA, Bacterial / Q000302:isolation & purification; D008040:Genetic Linkage; D005838:Genotype; D009154:Mutation; D010641:Phenotype; D011743:Pyrimidines / Q000096:biosynthesis*; D011995:Recombination, Genetic; D014170:Transformation, Genetic; D014498:Uracil / Q000378:metabolism*
D003470:Culture Media; D004269:DNA, Bacterial; D011743:Pyrimidines; D014498:Uracil