A possible ribosomal-directed regulatory system in Euglena gracilis. Chlorophyll synthesis.
Cycloheximide at concentrations of 0.1-100mum stimulated chlorophyll synthesis when dark-grown cells of Euglena were illuminated. Chloramphenicol (1-4mm) inhibited chlorophyll synthesis. The effect of cycloheximide on the incorporation of [(14)C]leucine into material insoluble in trichloroacetic acid, and its failure to affect the incorporation of [(32)P]orthophosphate into such material in short incubations, are interpreted as evidence that cycloheximide specifically inhibits protein synthesis by 80S ribosomes. Since the inhibitory effect of chloramphenicol on chlorophyll synthesis is counteracted by the presence of cycloheximide, it is suggested that chlorophyll synthesis is subject to control by a cytoplasmic repressor synthesized on 80S ribosomes, and to a de-repressor synthesized on 70S ribosomes.
D002247:Carbon Isotopes; D002701:Chloramphenicol / Q000494:pharmacology; D002734:Chlorophyll / Q000096:biosynthesis*; D003513:Cycloheximide / Q000494:pharmacology; D003593:Cytoplasm; D003624:Darkness; D003864:Depression, Chemical; D004794:Enzyme Repression; D005056:Euglena gracilis / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D010759:Phosphorus Isotopes; D014176:Protein Biosynthesis; D012270:Ribosomes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D012995:Solubility; D013997:Time Factors; D014238:Trichloroacetic Acid
D002247:Carbon Isotopes; D010710:Phosphates; D010759:Phosphorus Isotopes; D002734:Chlorophyll; D014238:Trichloroacetic Acid; D002701:Chloramphenicol; D003513:Cycloheximide; D007930:Leucine