Studies on the fluorophore sempervirene and its complexes with DNA.
Evidence that the fluorophore sempervirene binds to nucleic acids is presented. The complexes were studied by fluorescence intensity, spectra, decay lifetime, and polarization methods. Both fluorescent and nonfluorescent complexes are formed. The sempervirene is rigidly fixed to DNA. If ethidium bromide and sempervirene are bound to DNA, energy can be transferred from sempervirene to ethidium. Sempervirene is taken up by mammalian cells and appears in the cytoplasm. This unusual new probe should be useful in molecular and cellular investigations.
D000818:Animals; D001665:Binding Sites; D001854:Bone Marrow Cells; D002417:Cattle; D002460:Cell Line; D006224:Cricetinae; D004247:DNA; D004492:Edetic Acid; D004996:Ethidium; D007668:Kidney; D008433:Mathematics; D008856:Microscopy, Fluorescence; D008859:Microscopy, Polarization; D009690:Nucleic Acid Conformation; D046948:Secologanin Tryptamine Alkaloids; D012965:Sodium Chloride; D013050:Spectrometry, Fluorescence; D013997:Time Factors
D046948:Secologanin Tryptamine Alkaloids; D012965:Sodium Chloride; D004247:DNA; D004492:Edetic Acid; D004996:Ethidium