Kinetics of RNA labeling in toad bladder epithelium: effects of aldosterone and related steroids.
Sucrose gradient analysis revealed an aldosterone-dependent increase in the incorporation of [(3)H]uridine into a nonmethylated, rapidly labeled RNA that sedimented at 9-12 S. This effect was antagonized by an anti-mineralocorticoid, spironolactone (SC 9420), and was not elicited either by a glucocorticoid, cortisol, or by the inactive isomer, 17-alpha-isoaldosterone. These findings are in accord with the inference that mRNA is induced by aldosterone during the latent period and that the induced mRNA mediates the action on sodium transport.
D000450:Aldosterone / Q000031:analogs & derivatives / Q000494:pharmacology*; D000818:Animals; D001001:Anura; D001693:Biological Transport, Active / Q000187:drug effects; D002024:Bufo marinus; D002250:Carbon Radioisotopes; D004848:Epithelium / Q000378:metabolism; D005260:Female; D006854:Hydrocortisone / Q000494:pharmacology; D007700:Kinetics; D008715:Methionine / Q000378:metabolism; D000451:Mineralocorticoid Receptor Antagonists; D012313:RNA / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D012964:Sodium / Q000378:metabolism; D013148:Spironolactone / Q000494:pharmacology; D013268:Stimulation, Chemical; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D001743:Urinary Bladder / Q000378:metabolism*
D002250:Carbon Radioisotopes; D000451:Mineralocorticoid Receptor Antagonists; D012333:RNA, Messenger; D014316:Tritium; D013148:Spironolactone; D000450:Aldosterone; D012313:RNA; D012964:Sodium; D008715:Methionine; D014529:Uridine; D006854:Hydrocortisone