Characterization of three plasmid deoxyribonucleic acid molecules in a strain of Streptococcus faecalis: identification of a plasmid determining erythromycin resistance.
Three plasmids designated alpha, beta, and gamma, distinguishable by their molecular weights (6, 17, and 34 million, respectively) were isolated from Streptococcus faecalis strain DS-5 (ATCC 14508). Derivatives of this strain "cured" for erythromycin resistance lacked the beta-plasmid. In the parent strain the beta-plasmid was estimated to be present to the extent of one to two copies per chromosomal genome equivalent whereas the alpha- and gamma-plasmids were about nine and five copies, respectively.
D000166:Acridines / Q000494:pharmacology; D002250:Carbon Radioisotopes; D002499:Centrifugation, Density Gradient; D004269:DNA, Bacterial / Q000032:analysis* / Q000302:isolation & purification; D004270:DNA, Circular / Q000302:isolation & purification; D004352:Drug Resistance, Microbial; D013293:Enterococcus faecalis / Q000032:analysis / Q000187:drug effects*; D004917:Erythromycin / Q000494:pharmacology*; D005111:Extrachromosomal Inheritance; D008034:Lincomycin / Q000494:pharmacology; D008854:Microscopy, Electron; D008970:Molecular Weight; D013501:Surface-Active Agents; D013752:Tetracycline / Q000494:pharmacology; D013936:Thymidine; D014316:Tritium
D000166:Acridines; D002250:Carbon Radioisotopes; D004269:DNA, Bacterial; D004270:DNA, Circular; D013501:Surface-Active Agents; D014316:Tritium; D004917:Erythromycin; D008034:Lincomycin; D013752:Tetracycline; D013936:Thymidine