Preparation of solid-phase immunosorbents by coupling human serum proteins to cyanogen bromide-activated agarose.
The method of preparing solid-phase immunosorbents by covalently attaching proteins from whole human serum to cyanogen bromide-activated agarose has been investigated to determine optimum concentrations of cyanogen bromide and protein, and the optimum pH for the maximum attachment of proteins from serum. Two systems in which the above immunosorbents have proved useful are described: the removal of antibodies to normal serum proteins from anti-hepatitis B serum and the removal of light chain antibodies from anti-human immunoglobulin M serum.
D000042:Absorption; D000818:Animals; D001798:Blood Proteins; D003488:Cyanogen Bromide; D006041:Goats / Q000276:immunology; D006505:Hepatitis / Q000276:immunology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera; D007120:Immunochemistry; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007075:Immunoglobulin M; D010949:Plasma; D011134:Polysaccharides; D012709:Serum Albumin; D008258:Waldenstrom Macroglobulinemia / Q000097:blood
D001798:Blood Proteins; D007106:Immune Sera; D007075:Immunoglobulin M; D011134:Polysaccharides; D012709:Serum Albumin; D003488:Cyanogen Bromide