Antigens of Streptococcus mutans. II. Characterization of an antigen resembling a glycerol teichoic acid in walls of strain BHT.
Cold 10% trichloroacetic acid was used to extract antigens from purified cell walls of Streptococcus mutans BHT. Column chromatography on Biogel P-100 resolved two serologically reactive fractions (B and C). These fractions were ascertained to be relatively pure by recycling on Biogel P-100, Ouchterlony double-diffusion analysis, and immunoelectrophoresis. Fractions B and C demonstrated bands of absolute homology by double-diffusion but different mobilities by immunoelectrophoresis. Chemical analysis indicated that fraction B is a polysaccharide composed principally of rhamnose and galactose, with smaller amounts of glucose and glucosamine. Small quantities of glycerol and phosphorus also were found. Fraction C was composed mainly of galactose, glycerol, and phosphorus. Alkaline hydrolysis of this fraction yielded products typically released by the degradation of a glycerol teichoic acid, such as glycerol monophosphate, glycerol diphosphate, inorganic phosphorus, and several glycosyl glycerol phosphates. Diglycerol triphosphate was not detected. Side-group analysis revealed that glycerol was substituted by mono- and trigalactosyl moieties. Fraction C was deduced to contain 25 glycerol phosphate units per polymer length. Hapten inhibition studies revealed a beta-galactoside as the probable hapten on this antigen. The BHT teichoic acid reacted strongly with FA-1 antiserum. It showed bands of homology with both BHT and FA-1 crude acid extracts upon double-diffusion, using antisera to either strain. The BHT teichoic acid also displayed immunoelectrophoretic behavior identical to one of the mobile FA-1 cell wall antigens, again using either serum to develop precipitin bands. It is concluded this antigen may possess a serotype-specific determinant for S. mutans serotype b.
D000178:Acrylamides; D000469:Alkaline Phosphatase; D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D001431:Bacteriological Techniques; D002473:Cell Wall / Q000276:immunology; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D005990:Glycerol / Q000032:analysis*; D007106:Immune Sera; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D010758:Phosphorus / Q000032:analysis; D011233:Precipitin Tests; D011817:Rabbits / Q000276:immunology; D012703:Serotyping; D013291:Streptococcus / Q000276:immunology*; D013682:Teichoic Acids / Q000032:analysis*; D014238:Trichloroacetic Acid
D000178:Acrylamides; D000942:Antigens, Bacterial; D007106:Immune Sera; D013682:Teichoic Acids; D010758:Phosphorus; D014238:Trichloroacetic Acid; D000469:Alkaline Phosphatase; D005990:Glycerol