Polyribosomal and particulate distribution of lysyl- and phenylalanyl-transfer ribonucleic acid synthetases.
1. Only two aminoacyl-tRNA synthetases from Chinese hamster ovary cells are found associated with ribosomes and polyribosomes. 2. Phenylalanyl-tRNA synthetase activity is found with the 60S subunit, 80S monoribosome and individual polyribosomes. An additional 15S form of the enzyme is also seen. 3. Lysyl-tRNA synthetase activity is found in a form of about 20S and associated with ribosomal subunits and polyribosomes. The ribosomal subunits having lysyl-tRNA synthetase activity are about 6S larger than the bulk of the ribosomal subunits. 4. The lysyl- and phenylalanyl-tRNA synthetases found in different complexes have differential sensitivity to EDTA and centrifugation properties.
D000596:Amino Acids; D000604:Amino Acyl-tRNA Synthetases / Q000032:analysis*; D000818:Animals; D001665:Binding Sites; D002250:Carbon Radioisotopes; D002458:Cell Fractionation; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D004492:Edetic Acid; D005260:Female; D010053:Ovary / Q000201:enzymology; D011132:Polyribosomes / Q000201:enzymology*; D012343:RNA, Transfer; D012270:Ribosomes / Q000201:enzymology*
D000596:Amino Acids; D002250:Carbon Radioisotopes; D012343:RNA, Transfer; D004492:Edetic Acid; D000604:Amino Acyl-tRNA Synthetases