Studies on the bacteriophage 2 receptors of Pseudomonas aeruginosa.
The lysogenization of Pseudomonas aeruginosa strain BI with phage 2 resulted in the loss of the capacity to adsorb the same phage. The absence of phage 2 receptors on the surface of the lysogenized strain BI(2)(8) was confirmed by the failure of purified slime polysaccharide (SPB) or lipopolysaccharide (LPS) to inactivate phage 2. SPB and LPS from a phage 2-resistant strain also failed to inactivate phage 2 in contrast to the phage inactivation exhibited by the SPB and LPS obtained from the wild-type strain BI. Chemically, quantitative differences were apparent when the SPB and LPS of strains BI(2)(8) and BI/2S(2) were compared with those of the wild-type strain BI. The most striking difference noted was the absence of amino sugars in the SPB of strain BI/2S(2). The SPB of strain BI(2)(8) also contained a lower percentage of amino sugars compared with the SPB of the wild-type strain BI.
D000327:Adsorption; D000606:Amino Sugars / Q000032:analysis; D001435:Bacteriophages / Q000254:growth & development; D001665:Binding Sites; D002854:Chromatography, Paper; D008070:Lipopolysaccharides / Q000032:analysis; D008242:Lysogeny; D009154:Mutation; D011135:Polysaccharides, Bacterial / Q000032:analysis; D011550:Pseudomonas aeruginosa / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet
D000606:Amino Sugars; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial