Glycyl-L-leucine hydrolase, a versatile 'master' dipeptidase from monkey small intestine.
1. A highly active and electrophoretically homogeneous dipeptidase was purified from the soluble extracts of monkey small-intestinal mucosa. 2. By gel-filtration studies the molecular weight of the enzyme was found to be 107000. It is composed of two identical, subunits of molecular weight 54000. 3. A paper-chromatographic method of dipeptidase assay was developed to overcome some of the difficulties encountered in the generally used spectrophotometric procedure. By using this method, the K(m) and k(0) values of a few substrates were determined. 4. The substrate specificity of the enzyme was investigated in great detail with substrates of a wide range of possible structural types. The enzyme hydrolyses a very large proportion of the range of dipeptides tested. This enzyme, which exhibits such a wide range of action, has been termed the ;master' dipeptidase of the intestine. Glycylglycine, glycyl-l-proline, glycyl-l-histidine, l-prolylglycine and some of the arginine- and aspartic acid-containing dipeptides were not substrates and are possibly hydrolysed by other peptidases. These results therefore suggest that in the intestine the number of dipeptidases is rather limited. 5. In the light of these findings, the implications on the role of dipeptidases in intestinal peptide transport are discussed.
D000818:Animals; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D004150:Dipeptidases / Q000302:isolation & purification / Q000378:metabolism*; D004589:Electrophoresis, Disc; D005998:Glycine; D000882:Haplorhini; D006863:Hydrogen-Ion Concentration; D007421:Intestine, Small / Q000201:enzymology*; D007700:Kinetics; D007930:Leucine; D008970:Molecular Weight; D013053:Spectrophotometry; D013329:Structure-Activity Relationship
D004150:Dipeptidases; C020825:cytosol non-specific dipeptidase; D007930:Leucine; D005998:Glycine