Phosphate-induced protein chromatography.
High phosphate concentration is shown to cause a large proportion of the proteins of a cell-free extract of Escherichia coli to bind to agarose columns to which L-valine is attached. With a decreasing concentration gradient of potassium phosphate, the proteins elute in relation to their solubility in concentrated ammonium sulfate. This column technique appears to provide a general tool for the purification of proteins.
D001412:Bacillus subtilis; D002474:Cell-Free System; D002850:Chromatography, Gel; D004926:Escherichia coli; D005696:Galactosidases / Q000302:isolation & purification; D006836:Hydro-Lyases / Q000302:isolation & purification; D008722:Methods; D010710:Phosphates; D011134:Polysaccharides; D011188:Potassium; D011506:Proteins / Q000302:isolation & purification*; D012995:Solubility; D013307:Streptomycin; D013912:Threonine; D014633:Valine
D010710:Phosphates; D011134:Polysaccharides; D011506:Proteins; D013912:Threonine; D005696:Galactosidases; D006836:Hydro-Lyases; D014633:Valine; D011188:Potassium; D013307:Streptomycin