Immunogical study of anti-beta-lactamase antibodies by acidimetric methods.
A beta-lactamase was extracted from an Escherichia coli K-12 strain carrying the R-TEM plasmid and has been purified by affinity chromatography. Antisera to this enzyme were prepared in the rabbit, and the enzyme-antibody neutralization reaction has been evaluated with acidimetric methods (pH stat or pH meter). Under defined experimental conditions, it is now possible to clearly illustrate the enzyme-antibody reaction by means of accurate, rapid, and simple-to-perform methods. These methods are in accord with the specificity of the two reactive partners and allow the detection of the enzyme in a crude bacterial extract which would eventually contain more than one beta-lactamase.
D000818:Animals; D000907:Antibodies, Bacterial; D000918:Antibody Specificity; D002846:Chromatography, Affinity; D004926:Escherichia coli / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D007122:Immunoelectrophoresis; D008722:Methods; D009500:Neutralization Tests; D010405:Penicillinase / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000201:enzymology; D011817:Rabbits / Q000276:immunology
D000907:Antibodies, Bacterial; D010405:Penicillinase