Energy dispersion x-ray analysis by scanning electron microscopy for measuring cellular elemental composition in bacterial cells.
The use of scanning electron microscopy in conjuction with energy dispersion analysis of X rays is presented as a technique in a novel application to in situ studies of cellular elemental composition. The method has been applied, in the example of its use reported here, to examining the effects of copper-chromearsenic wood preservative on growth and element uptake of selected bacteria in agar cultures.
D001151:Arsenic / Q000032:analysis; D001407:Bacillus / Q000378:metabolism; D001412:Bacillus subtilis / Q000032:analysis; D001419:Bacteria / Q000032:analysis*; D002118:Calcium / Q000032:analysis; D002713:Chlorine / Q000032:analysis; D002857:Chromium / Q000032:analysis; D003300:Copper / Q000032:analysis; D003470:Culture Media; D004735:Energy Transfer; D008722:Methods; D008855:Microscopy, Electron, Scanning; D010758:Phosphorus / Q000032:analysis; D011188:Potassium / Q000032:analysis; D011550:Pseudomonas aeruginosa / Q000032:analysis / Q000378:metabolism; D012964:Sodium / Q000032:analysis; D013291:Streptococcus / Q000032:analysis; D013455:Sulfur / Q000032:analysis
D003470:Culture Media; D002857:Chromium; D010758:Phosphorus; D002713:Chlorine; D013455:Sulfur; D003300:Copper; D012964:Sodium; D001151:Arsenic; D011188:Potassium; D002118:Calcium