Effects of mutational loss of specific intracellular proteases on the sporulation of Bacillus subtilis.
Two protease-deficient mutants of Bacillus subtilis 168 (Trp(-)) were isolated and compared with the parental strain with respect to production of intracellular proteases and sporulation. A mutant lacking the metal-requiring "neutral" protease intracellularly sporulated as well as the parental strain. A second mutant, deficient in an as yet uncharacterized intracellular protease, failed to sporulate normally. It is proposed that this new protease is also involved in intracellular protein turnover.
D000942:Antigens, Bacterial / Q000032:analysis; D001412:Bacillus subtilis / Q000201:enzymology* / Q000254:growth & development / Q000276:immunology; D002364:Caseins / Q000378:metabolism; D002474:Cell-Free System; D004492:Edetic Acid / Q000494:pharmacology; D004586:Electrophoresis; D005779:Immunodiffusion; D009154:Mutation; D010447:Peptide Hydrolases / Q000096:biosynthesis* / Q000378:metabolism; D013170:Spores / Q000254:growth & development*; D013171:Spores, Bacterial / Q000254:growth & development
D000942:Antigens, Bacterial; D002364:Caseins; D004492:Edetic Acid; D010447:Peptide Hydrolases