Lipid metabolism of monkey kidney cells (LLC-MK-2) infected with Chlamydia trachomatis strain lymphogranuloma venereum.
Lipid metabolism of monkey kidney (LLC-MK-2) cells and cells infected with a Chlamydia trachomatis strain lymphogranuloma venereum (LGV) was studied. The protein-to-lipid ratio of normal MK-2 cells was found to increase linearly over a 60-h period of incubation. The protein-to-lipid ratio of the infected cells was similar to that in normal cells until 36 h after infection, when a plateau in the ratio was observed. Lipid synthesis of the infected cells was found to be inhibited after 48 h of infection. Turnover of host lipids did not appear to be markedly altered by infection with LGV over a 48-h period of incubation. An anteiso branched chain of 15:0 fatty acid was found in infected cells but not in normal cells. The appearance of this fatty acid, correlated with a rise in the infectivity of LGV, suggests that synthesis of specific lipids was associated with the infection.
D000085:Acetates / Q000378:metabolism; D000818:Animals; D002250:Carbon Radioisotopes; D002460:Cell Line; D002689:Chlamydia / Q000302:isolation & purification; D003470:Culture Media; D046508:Culture Techniques; D004952:Esters; D005227:Fatty Acids / Q000032:analysis; D005233:Fatty Alcohols; D006100:Granuloma Inguinale / Q000378:metabolism* / Q000382:microbiology; D000882:Haplorhini; D007668:Kidney / Q000378:metabolism*; D050356:Lipid Metabolism; D008055:Lipids / Q000302:isolation & purification; D011506:Proteins / Q000032:analysis; D010948:Viral Plaque Assay; D014776:Virus Cultivation
D000085:Acetates; D002250:Carbon Radioisotopes; D003470:Culture Media; D004952:Esters; D005227:Fatty Acids; D005233:Fatty Alcohols; D008055:Lipids; D011506:Proteins