C4-dicarboxylate transport in Bacillus subtilis studied with 3-fluoro-L-erythro-malate as a substrate.
Bacillus subtilis cells grown in yeast extract medium accumulated 3-fluoro-l-erythro-[1,2-(14)C(2)]malate more than 30-fold from the surrounding medium. No metabolic products derived from 3-fluoro-l-erythro-malate could be detected in these cells. l-Malate competitively inhibited transport of 3-fluoro-l-erythro-malate. This malate analogue was itself a competitive inhibitor of l-malate uptake. Cells that had been grown in yeast extract supplemented with 5 mM l-malate showed a 10-fold increased affinity towards 3-fluoro-l-erythro-malate relative to cells grown in yeast extract medium with no added malate. Our results suggest that two transport systems for l-malate can be induced in B. subtilis. The first of these systems seems to effect uptake of C(4)-dicarboxylates (l-malate, succinate, and fumarate) in yeast extract medium. The second transport system (or possibly a modification of the first transport system) seems to be induced by addition of l-malate to this medium and is also functioning in malate minimal medium.
D001345:Autoradiography; D001412:Bacillus subtilis / Q000254:growth & development / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002854:Chromatography, Paper; D003470:Culture Media; D003998:Dicarboxylic Acids / Q000378:metabolism*; D005461:Fluorine; D005650:Fumarates / Q000494:pharmacology; D008293:Malates / Q000138:chemical synthesis / Q000378:metabolism* / Q000494:pharmacology; D013237:Stereoisomerism; D013386:Succinates / Q000494:pharmacology; D013644:Tartrates / Q000494:pharmacology; D013997:Time Factors
D002250:Carbon Radioisotopes; D003470:Culture Media; D003998:Dicarboxylic Acids; D005650:Fumarates; D008293:Malates; D013386:Succinates; D013644:Tartrates; D005461:Fluorine; C007752:3-fluoromalic acid