Vaccinia virus replication in enucleate BSC-1 cells: particle production and synthesis of viral DNA and proteins.
The growth of vaccinia virus in monolayers of BSC-1 cells enucleated by centrifugation in the presence of cytochalasin B has been studied. No evidence for the production of infectious virus in these cells was obtained, and the production of virus particles was reduced to 8.3% compared with the yield from cytochalasin-treated, uncentrifuged cells. Virus DNA and early and late polypeptides were synthesized with normal timing in enucleate cells, but in reduced amounts; cleavage of structural polypeptide precursors P4a and Px also occurred in enucleate cells. Factories containing immature virus particles were demonstrated in enucleate cells by electron microscopy; these factories were reduced in number and size compared with those found in cytochalasin-treated, uncentrifuged cells.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D002250:Carbon Radioisotopes; D002460:Cell Line; D002467:Cell Nucleus / Q000382:microbiology; D002478:Cells, Cultured / Q000382:microbiology*; D002499:Centrifugation, Density Gradient; D003571:Cytochalasin B / Q000494:pharmacology; D003593:Cytoplasm / Q000382:microbiology; D004279:DNA, Viral / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D000882:Haplorhini; D007668:Kidney; D008715:Methionine / Q000378:metabolism; D008854:Microscopy, Electron; D010452:Peptide Biosynthesis; D011498:Protein Precursors / Q000378:metabolism; D013462:Sulfur Radioisotopes; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014616:Vaccinia virus / Q000254:growth & development* / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis*; D014779:Virus Replication
D000596:Amino Acids; D002250:Carbon Radioisotopes; D004279:DNA, Viral; D011498:Protein Precursors; D013462:Sulfur Radioisotopes; D014764:Viral Proteins; D014316:Tritium; D003571:Cytochalasin B; D008715:Methionine; D013936:Thymidine