The formation of plaques by African horse sickness viruses and factors affecting plaque size.
Under agar overlay, large and small plaques were observed for African Horse Sickness viruses. The purification of large plaques by cloning was possible. By addition of protamine sulfate to the agar overlay only large plaques were produced. The plaque size variation can not be a suitable marker for determination of variants of this virus since under methyl cellulose and starch gel overlays homogenous plaques were noticed.
D000818:Animals; D003470:Culture Media; D051379:Mice; D011479:Protamines / Q000494:pharmacology; D014776:Virus Cultivation; D014713:Viruses, Unclassified
D003470:Culture Media; D011479:Protamines