In vitro synthesis of ribosomal proteins directed by Escherichia coli DNA.
In vitro synthesis of a number of E. coli 30S ribosomal proteins has been demonstrated in a cell-free system consisting of ribosomes, initiation factors, RNA polymerase, a fraction containing soluble enzymes and factors, and E. coli DNA. DNA-dependent synthesis of the following 30S proteins has been demonstrated: S4, S5, S7, S8, S9, S10, S13, S14, S16, S19, and S20.
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D002474:Cell-Free System; D003090:Coliphages; D004269:DNA, Bacterial / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D004586:Electrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D005779:Immunodiffusion; D008715:Methionine / Q000378:metabolism; D010448:Peptide Initiation Factors; D010455:Peptides / Q000032:analysis; D011863:Radioimmunoassay; D012270:Ribosomes / Q000378:metabolism*; D012293:Rifampin; D013462:Sulfur Radioisotopes; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004279:DNA, Viral; D010448:Peptide Initiation Factors; D010455:Peptides; D013462:Sulfur Radioisotopes; D008715:Methionine; D012321:DNA-Directed RNA Polymerases; D003851:Deoxyribonucleases; D012293:Rifampin