Specimen holder to critical-point dry microorganisms for scanning electron microscopy.
Critical-point drying of microorganisms for scanning electron microscopy can be rapidly and effectively accomplished by use of a newly described specimen holder. Up to eight different samples of spores or vegetative cells are placed between polycarbonate membrane filters in the holder and processed through solvent dehydration and critical-point drying using carbon dioxide without loss or cross contamination of microorganisms. Yeasts, molds, bacteria, and actinomycetes have been successfully processed.
D001203:Ascomycota / Q000648:ultrastructure; D001409:Bacillus cereus / Q000648:ultrastructure; D001419:Bacteria / Q000648:ultrastructure*; D002245:Carbon Dioxide; D005612:Freeze Drying / Q000295:instrumentation*; D005658:Fungi / Q000648:ultrastructure*; D008851:Micropore Filters; D008855:Microscopy, Electron, Scanning; D012233:Rhizopus / Q000648:ultrastructure; D012440:Saccharomyces / Q000648:ultrastructure; D013171:Spores, Bacterial / Q000648:ultrastructure; D013172:Spores, Fungal / Q000648:ultrastructure; D013302:Streptomyces / Q000648:ultrastructure; D013305:Streptomyces griseus / Q000648:ultrastructure
D002245:Carbon Dioxide