Characterization of a bacteriophage-induced, host-specific lytic enzyme from lysates of Bacillus stearothermophilus infected with bacteriophage TP-8.
Phage TP-8 lysates of Bacillus stearothermophilus 4S or 4S(8) contain lytic activity exhibiting two pH optima, one at pH 6.5 and the other at pH 7.5. Using a variety of fractionation procedures, the two lytic activities could not be separated. At pH 7.5 the lytic enzyme is an endopeptidase which hydrolyzes the l-alanyl-d-glutamyl linkage in the peptide subunits of the cell wall peptidoglycan and at pH 6.5 it exhibits N-acetylmuramidase activity. Endopeptidase activity is inhibited by NaCl and neither lytic activity was significantly affected by divalent cations or ethylenediaminetetraacetic acid. Crude lysates contain 2.5 to 3.0 times more endopeptidase activity than N-acetylmuramidase activity. The ratio of the two lytic activities (endopeptidase/N-acetylmuramidase) changes to 1.3 to 1.7 during the course of purification, to 1.0 after isoelectric focusing, and 3.9 and 6.00 after exposure for 2 h at 60 and 65 C, respectively. We conclude that the two lytic activities may be associated with a single protein or a lytic enzyme complex composed of two enzymes. Lytic activity at pH 7.5 is more effective in solubilizing cells or cell walls than the lytic activity at pH 6.5. LiCl extracts of 4S and 4S(8) cells contain lytic activity exhibiting endopeptidase activity at pH 7.5 and N-acetylmuramidase activity at pH 6.5. Lytic activity in these LiCl extracts also has a number of other properties in common with those in lysates of phage TP-8. We proposed that the lytic enzyme(s) are not coded for by the phage genome but are part of the host autolytic system.
D001407:Bacillus / Q000201:enzymology*; D001435:Bacteriophages; D002021:Buffers; D002458:Cell Fractionation; D002473:Cell Wall / Q000032:analysis / Q000187:drug effects; D002848:Chromatography, DEAE-Cellulose; D003470:Culture Media; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007700:Kinetics; D008094:Lithium; D008937:Mitomycins / Q000494:pharmacology; D010447:Peptide Hydrolases / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013053:Spectrophotometry; D014461:Ultracentrifugation
D002021:Buffers; D003470:Culture Media; D008937:Mitomycins; D012965:Sodium Chloride; D008094:Lithium; D004492:Edetic Acid; D010447:Peptide Hydrolases