Purification and characterization of Streptococcus mutans group d cell wall polysaccharide antigen.
The Streptococcus mutans group d antigen of strain B13 has been purified and characterized with respect to chemical composition and immunochemical properties. The antigen was extracted from lyophilized cells or cell walls by using 5% trichloroacetic acid at 5 C for 16 h. The antigen could also be extracted with water or 0.01 N HCl at 100 C for 20 min. The antigen was purified by ion-exchange and gel chromatography and was found to contain 96% carbohydrate, 1.6% protein, and 0.3% phosphorus. Characterization by gas chromatography indicated that the polysaccharide was composed of galactose and glucose in a 2:1 ratio. The antigen contained two serologically active sites: one site specific for group d and a second site common to both group d and group a strains. Agar diffusion and immunoelectrophoresis indicated that the two sites existed on a single molecule. The immunological specificity of the group d polysaccharide site depended on a terminal d-galactose. The purified B13 antigen did not react with antisera specific for the glycerol teichoic acid from streptococci. Anti-d serum rapidly agglutinated whole cells, indicating that the antibody receptor sites of the polysaccharide antigen were at the surface of the streptococcal cell.
D000327:Adsorption; D000429:Alcohol Oxidoreductases / Q000494:pharmacology; D000818:Animals; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D002241:Carbohydrates / Q000032:analysis; D002473:Cell Wall / Q000276:immunology; D002480:Cellulase; D002849:Chromatography, Gas; D003910:Dextranase / Q000494:pharmacology; D005690:Galactose; D005696:Galactosidases / Q000494:pharmacology; D005949:Glucose Oxidase / Q000494:pharmacology; D005959:Glucosidases / Q000494:pharmacology; D007106:Immune Sera; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D009113:Muramidase / Q000494:pharmacology; D010758:Phosphorus / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D011233:Precipitin Tests; D011506:Proteins / Q000032:analysis; D011817:Rabbits / Q000276:immunology; D013291:Streptococcus / Q000276:immunology*; D013682:Teichoic Acids; D014357:Trypsin / Q000494:pharmacology
D000942:Antigens, Bacterial; D002241:Carbohydrates; D007106:Immune Sera; D011135:Polysaccharides, Bacterial; D011506:Proteins; D013682:Teichoic Acids; D010758:Phosphorus; D000429:Alcohol Oxidoreductases; D005949:Glucose Oxidase; D005696:Galactosidases; D005959:Glucosidases; D003910:Dextranase; D009113:Muramidase; D002480:Cellulase; D014357:Trypsin; D005690:Galactose