Freeze-substitution: a method for the rapid arrest and chemical fixation of spermatozoa.
D000096:Acetone; D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D005617:Chlorofluorocarbons, Methane; D003102:Colloids; D005026:Ethylene Glycols; D005069:Evaluation Studies as Topic; D005407:Flagella / Q000648:ultrastructure; D005557:Formaldehyde; D005615:Freezing; D005976:Glutaral; D006652:Histological Techniques; D008297:Male; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D009992:Osmium; D051381:Rats; D012997:Solvents; D013094:Spermatozoa / Q000648:ultrastructure*
D005617:Chlorofluorocarbons, Methane; D003102:Colloids; D005026:Ethylene Glycols; D012997:Solvents; D000096:Acetone; D005557:Formaldehyde; D009992:Osmium; D005976:Glutaral