Replication of bacteriophage SH-133 in the facultative autotroph Hydrogenomonas facilis. II. Bacteriophage synthesis and hydrogenase induction under step-up and step-down growth conditions.
Autotrophically grown infected cells are able to replicate phage SH-133 after being switched to a heterotrophic environment (step-up growth). The effect of step-down growth on phage replication varies with the choice of organic substrate. Phage replication and the induction of cellular hydrogenase occur under step-down growth from acetate but not peptone broth. The requirement of a continued source of energy for phage replication in either heterotrophically or autotrophically grown cells could be uniquely demonstrated in this phage-host system by the deletion of hydrogen from the growth medium.
D001435:Bacteriophages / Q000201:enzymology*; D002478:Cells, Cultured; D003470:Culture Media; D004790:Enzyme Induction; D010088:Oxidoreductases / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology* / Q000254:growth & development; D013997:Time Factors; D010948:Viral Plaque Assay; D014779:Virus Replication
D003470:Culture Media; D010088:Oxidoreductases