Spermidine biosynthesis during germination and subsequent vegetative growth of Bacillus megaterium spores.
Spermidine biosynthesis was extremely low early in germination of Bacillus megaterium spores and the spermidine level remained constant. Rapid synthesis began after 130 min and thereafter accounted for the increase in spermidine level which began at this time. Biosynthesis was greatly (>84%) diminished by exogenous spermine or spermidine. Arginine and ornithine were both converted efficiently into spermidine, but arginine was the more immediate precursor as shown by isotope competition studies and by the absence of ornithine decarboxylase and the presence of arginine decarboxylase. Exogenous putrescine was not incorporated into spermidine, although it was taken up rapidly and degraded.
D001120:Arginine / Q000378:metabolism; D001410:Bacillus megaterium / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D002172:Canavanine / Q000494:pharmacology; D002250:Carbon Radioisotopes; D002262:Carboxy-Lyases / Q000378:metabolism; D003864:Depression, Chemical; D009952:Ornithine / Q000378:metabolism; D011700:Putrescine / Q000378:metabolism; D013095:Spermidine / Q000096:biosynthesis* / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology; D013171:Spores, Bacterial / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D013997:Time Factors; D014316:Tritium
D002250:Carbon Radioisotopes; D014316:Tritium; D013096:Spermine; D002172:Canavanine; D001120:Arginine; D009952:Ornithine; D002262:Carboxy-Lyases; D013095:Spermidine; D011700:Putrescine