The use of immunoadsorbent columns for the isolation of antibodies specific for antigens associated with human bronchogenic carcinoma.
An immunoadsorbent technique is described whereby tumour-specific antibodies may be isolated. Extracts from normal human lung tissue were pooled and bound to cyanogen bromide activated Sepharose 4B. Antisera raised in rabbits to a variety of extracts from human bronchogenic carcinoma were passed through these immunoadsorbent columns to yield antisera specific for tumour-associated antigens as demonstrated by immunodiffusion and immunoelectrophoresis.
D000327:Adsorption; D000818:Animals; D000912:Antibodies, Neoplasm / Q000302:isolation & purification*; D000918:Antibody Specificity; D000951:Antigens, Neoplasm; D002283:Carcinoma, Bronchogenic / Q000276:immunology*; D003488:Cyanogen Bromide; D006801:Humans; D007106:Immune Sera / Q000302:isolation & purification; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008168:Lung / Q000276:immunology; D008175:Lung Neoplasms / Q000276:immunology*; D008722:Methods; D011817:Rabbits / Q000276:immunology
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D007106:Immune Sera; D003488:Cyanogen Bromide