Immunoglobulin molecules on the surface of activated T lymphocytes in the rat.
Lewis strain rat lymphocytes were exposed in vitro to a variety of specific and nonspecific blastogenic stimuli. The surfaces of the transformed lymphocytes were examined by indirect immunofluorescence for the presence of T cell antigens and immunoglobulin molecules. More than 90% of lymphocytes that underwent blast transformation after exposure to foreign histocompatibility antigens (mixed lymphocyte reaction; in vitro allograft reaction), purified tuberculin, phytohemagglutinin (PHA), and concanavalin A (Con-A) had T cell antigenic markers on their surfaces. 70-92% of the antigen-stimulated blast cells also had readily detectable surface immunoglobulin molecules, whereas less than 3% of the PHA- and Con-A-activated cells were Ig(+). Pokeweed mitogen (PWM) appeared to activate both B and T cells, but the T cells did not have detectable surface immunoglobulin molecules. Nonactivated control cultures contained T(+)Ig(-) lymphocytes almost exclusively. The results suggest that thymus-dependent rat lymphocytes express increased amounts of detectable immunoglobulin on their surface in response to specific stimulation with antigen. It is postulated that the acquisition of immunological competence by activated T cells may be related to this expression of surface immunoglobulin.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D005260:Female; D005455:Fluorescent Antibody Technique; D005620:Freund's Adjuvant / Q000008:administration & dosage; D006649:Histocompatibility Antigens; D006736:Horses / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis; D007136:Immunoglobulins / Q000032:analysis*; D007668:Kidney / Q000276:immunology; D037102:Lectins / Q000494:pharmacology; D008213:Lymphocyte Activation; D008297:Male; D009169:Mycobacterium tuberculosis / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D051381:Rats; D011912:Rats, Inbred ACI; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology*; D014373:Tuberculin / Q000494:pharmacology
D006649:Histocompatibility Antigens; D007074:Immunoglobulin G; D007136:Immunoglobulins; D037102:Lectins; D014373:Tuberculin; D003208:Concanavalin A; D004247:DNA; D005620:Freund's Adjuvant