Culture of specimens other than sputum for Mycobacteria.
A comparison has been made of Lowenstein-Jensen medium, with or without added pyruvate, 7H11 oleic acid-albumin agar slopes made selective by the addition of antibiotics, and selective Kirchner medium in the isolation of tubercle bacilli from specimens other than sputum. A combination of a standard mild preliminary treatment with sulphuric acid and selective medium reduced the contamination rate to 0.1%. Neither acid treatment nor antibiotics appeared to reduce the isolation rate. An effective procedure is to treat all specimens (except cerebrospinal fluid) with acid and culture on two Lowenstein-Jensen slopes (one with pyruvate), a 7H11 selective slope, and in two bottles of selective Kirchner medium.
D000900:Anti-Bacterial Agents; D001431:Bacteriological Techniques / Q000379:methods; D001826:Body Fluids / Q000382:microbiology*; D002555:Cerebrospinal Fluid / Q000382:microbiology; D003470:Culture Media; D005260:Female; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D013183:Sputum / Q000382:microbiology; D013464:Sulfuric Acids; D013492:Suppuration / Q000382:microbiology; D014556:Urine / Q000382:microbiology
D000900:Anti-Bacterial Agents; D003470:Culture Media; D013464:Sulfuric Acids