Large surface blebs on Escherichia coli heated to inactivating temperatures.
Large surface blebs were observed with phase-contrast optics on Escherichia coli B/r and B(s-1) heated to temperatures at which colony-forming ability was lost. Characterization of such blebs was consistent with the view that they were formed by a physical process and were bounded by the outer membrane of the cell. A hypothesis for thermal inactivation of E. coli is presented that places membrane damage near the primary lethal event.
D002462:Cell Membrane / Q000187:drug effects; D002473:Cell Wall; D004492:Edetic Acid / Q000494:pharmacology; D004926:Escherichia coli / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development; D006358:Hot Temperature; D008858:Microscopy, Phase-Contrast; D009997:Osmotic Pressure; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D014050:Toluene / Q000494:pharmacology
D012710:Serum Albumin, Bovine; D012967:Sodium Dodecyl Sulfate; D014050:Toluene; D004492:Edetic Acid