Immunofluorescent study of the replication of infectious pancreatic necrosis virus in trout and Atlantic salmon cell cultures.
Cell cultures of trout gonad tissue (RTG-2) and Atlantic salmon heart, kidney, liver, and spleen tissue were inoculated with 50 50% tissue culture infective doses (TCID(50)) of infectious pancreatic necrosis (IPN) virus per cell, and the titer of cell-associated and released virus was determined from 2 to 16 h postinoculation (PI). Cover slips were collected over the same period and stained for IPN viral antigen by the direct immunofluorescent (FA) technique. Viral replication was detected after a latent period of approximately 2 to 4 h and reached a peak titer of 10(8.2) to 10(8.4) TCID(50) per ml at 8 to 10 h PI. The release of virus was more rapid in Atlantic salmon cells than in RTG-2 cells. Viral antigen was first detected by FA from 3 to 4 h PI. Approximately 75 to 80% of the cells contained antigen in the cytoplasm 9 to 11 h PI. The direct FA technique was found to be a sensitive method for detecting IPN virus in infected cells. Three strains of IPN virus were tested for serological cross reactions by FA and virus neutralization tests.
D000818:Animals; D002460:Cell Line; D046508:Culture Techniques; D005393:Fish Diseases / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006066:Gonads / Q000166:cytology; D007106:Immune Sera; D007668:Kidney / Q000166:cytology; D008099:Liver / Q000166:cytology; D009206:Myocardium / Q000166:cytology; D009500:Neutralization Tests; D011817:Rabbits / Q000276:immunology; D012487:Salmonidae; D013154:Spleen / Q000166:cytology; D014777:Virus Diseases / Q000276:immunology*; D014779:Virus Replication; D014780:Viruses / Q000276:immunology*
D007106:Immune Sera