Genetic location of two mutations affecting the lysyl-transfer ribonucleic acid synthetase of Bacillus subtilis.
Two mutations (lysS1 and lysS2), each independently resulting in a thermosensitive, lysyl-transfer RNA synthetase (l-lysine: tRNA ligase [adenosine 5'-monophosphate] EC 6.1.1.6), have been mapped on the Bacillus subtilis chromosome between purA16 (adenine requirement) and sul (sulfanilamide resistance). They are linked by transformation with sul (70 to 74% cotransfer) in the order purA16-lysS1-lysS2-sul. The mutant loci are either in the same gene or in two closely linked genes. They are not linked to the tryptophanyl-tRNA synthetase structural gene or to the lys-1 locus.
D000225:Adenine / Q000378:metabolism; D000604:Amino Acyl-tRNA Synthetases / Q000096:biosynthesis*; D001412:Bacillus subtilis / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D002874:Chromosome Mapping; D004352:Drug Resistance, Microbial; D005796:Genes; D008040:Genetic Linkage; D009154:Mutation; D011995:Recombination, Genetic; D013424:Sulfanilamides / Q000494:pharmacology; D013696:Temperature; D014161:Transduction, Genetic; D014170:Transformation, Genetic
D013424:Sulfanilamides; D000604:Amino Acyl-tRNA Synthetases; D000225:Adenine