Simple, inexpensive procedure for the disruption of bacteria.
Small volumes (1 to 2 ml) of bacterial cultures, with turbidities ranging from 3 to 10, were disrupted 50 to 90% (measured as a decrease in turbidity) within 2 min, by shaking them on a Vortex-type mixer in the presence of glass beads. This method of disrupton was effective for cells in the following genera: Streptococcus, Lactobacillus, Staphylococcus, Bacillus, and Escherichia. After low-speed centrifugation, the resulting extract can be used as potential sources for enzymes, transforming deoxyribonucleic acids, cell walls, membranes, etc.
D001412:Bacillus subtilis; D001419:Bacteria / Q000032:analysis*; D001431:Bacteriological Techniques; D002458:Cell Fractionation; D004269:DNA, Bacterial / Q000302:isolation & purification; D003720:Densitometry; D003851:Deoxyribonucleases / Q000378:metabolism; D004926:Escherichia coli; D007780:Lacticaseibacillus casei; D008722:Methods; D008854:Microscopy, Electron; D013210:Staphylococcus; D013291:Streptococcus; D014170:Transformation, Genetic; D014316:Tritium
D004269:DNA, Bacterial; D014316:Tritium; D003851:Deoxyribonucleases