Application of freeze-etching method to the study of reovirus-infected LLC-MK2 cells.
A continuous cell line of rhesus monkey kidney cells, LLC-MK(2), was infected with reovirus type 1 (Lang). The cells were freeze-etched as a method for observing the structural details of the reovirus-induced cytoplasmic inclusion. Information on maturation may be obtained by preparing infected cells for electron microscopy by freeze-etching.
D000818:Animals; D002244:Carbon; D002460:Cell Line; D046508:Culture Techniques; D005613:Freeze Etching; D005615:Freezing; D000882:Haplorhini; D006652:Histological Techniques; D007181:Inclusion Bodies, Viral; D007668:Kidney / Q000382:microbiology*; D008854:Microscopy, Electron; D010984:Platinum; D012087:Reoviridae / Q000302:isolation & purification* / Q000472:pathogenicity; D013194:Staining and Labeling
D010984:Platinum; D002244:Carbon