Mechanisms of selective uptake of 2-methyl-4-naphthoquinol bis disodium phosphate, into some mammalian cells in tissue culture.
Earlier papers have described the selective uptake of the radioactive drug 2-methyl 6,7-ditritio-1, 4-naphthoquinol bis disodium phosphate into some human tumour cells in tissue culture. This work examines these results in greater detail. The kinetics of the uptake process into HEp/2 cells and its precise dependence on the experimental conditions are described. It is shown that an alkaline phosphatase (APase) is present on the surface of HEp/2 cells and that this is responsible for the uptake of label. L cells do not take up the radioactive label and are shown not to possess significant quantities of APase.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002477:Cells / Q000187:drug effects; D002478:Cells, Cultured; D002729:Chloromercuribenzoates / Q000494:pharmacology; D003545:Cysteine / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007739:L Cells / Q000378:metabolism; D051379:Mice; D009285:Naphthoquinones / Q000378:metabolism*; D009374:Neoplasms, Experimental / Q000201:enzymology / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D014316:Tritium; D015032:Zinc / Q000494:pharmacology
D002729:Chloromercuribenzoates; D009285:Naphthoquinones; D010710:Phosphates; D014316:Tritium; D004492:Edetic Acid; D000469:Alkaline Phosphatase; D015032:Zinc; D003545:Cysteine