Screening and isolation of penicillinase inhibitor, KA-107.
It is known that penicillin resistance of bacteria is mainly caused by the inactivation of penicillin by penicillinase derived from such strains. We have developed a screening procedure for penicillinase inhibitors. Several microorganisms were found to produce such inhibitors, and from the culture filtrate of Streptomyces gedanensis ATCC 4880 a penicillinase inhibitor, named KA-107, was isolated. The characteristics of this inhibitor were revealed by an in vitro test by using penicillinase derived from penicillin resistant Staphylococcus aureus, FS-1277. When KA-107 was used in combination with penicillin-G, ampicillin, d- or l-phenethicillin, the growth inhibitory activity of these penicillins was maintained.
D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D001409:Bacillus cereus / Q000201:enzymology; D001431:Bacteriological Techniques; D003470:Culture Media; D004353:Drug Evaluation, Preclinical; D004357:Drug Synergism; D008826:Microbial Sensitivity Tests; D010403:Penicillin Resistance; D010405:Penicillinase / Q000302:isolation & purification; D013210:Staphylococcus / Q000201:enzymology; D013302:Streptomyces / Q000378:metabolism*; D065093:beta-Lactamase Inhibitors
D000900:Anti-Bacterial Agents; D003470:Culture Media; D065093:beta-Lactamase Inhibitors; C006044:KA-107; D010405:Penicillinase