Selective extraction of polymers from cell walls of gram-positive bacteria.
Brief heating of Bacillus Licheniformis cell walls at 100 degrees C in aqueous buffers of pH3.0-4.0 removes some polymers but not others from the mucopeptides. For example, relatively undegraded teichuronic acid can be extracted at 100 degrees C in 20min at pH3.0 whereas the teichoic acids are not removed. Similar specificity can be shown with walls from three other species of micro-organism.
D001407:Bacillus / Q000032:analysis* / Q000166:cytology; D001412:Bacillus subtilis / Q000032:analysis; D001419:Bacteria / Q000032:analysis*; D002473:Cell Wall / Q000032:analysis; D006020:Glycopeptides / Q000032:analysis; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008837:Micrococcus / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification*; D012995:Solubility; D013045:Species Specificity; D013291:Streptococcus / Q000032:analysis; D013682:Teichoic Acids / Q000302:isolation & purification; D013997:Time Factors; D014574:Uronic Acids / Q000032:analysis
D006020:Glycopeptides; D011135:Polysaccharides, Bacterial; D013682:Teichoic Acids; D014574:Uronic Acids