Comparative sensitivity of gel-diffusion and tube agglutination tests for the detection of Brucella canis antibodies in experimentally infected dogs.
The microslide gel-diffusion and macro-tube agglutination techniques to detect Brucella canis antibodies in dogs were compared. Sera from dogs experimentally infected with B. canis and a random sample of dog sera with unknown histories of exposure to this organism were examined. The results of the gel-diffusion method employing specific rough Brucella saline-extract antigens of B. canis and Brucella ovis were comparable to those obtained by the tube agglutination test. The easily prepared, stable R antigen in the freeze-dried form offers a convenient, simple, and reliable diagnostic method for the serological detection of canine brucellosis by the gel-diffusion test.
D000372:Agglutination Tests / Q000592:standards*; D000818:Animals; D000907:Antibodies, Bacterial / Q000302:isolation & purification*; D000942:Antigens, Bacterial; D002002:Brucella / Q000276:immunology*; D002006:Brucellosis / Q000662:veterinary*; D003937:Diagnosis, Differential; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D005069:Evaluation Studies as Topic; D005779:Immunodiffusion / Q000592:standards*; D008297:Male; D008722:Methods
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial