Induction of sporulation during synchronized chromosome replication in Bacillus subtilis.
Synchronous chromosome replication was obtained in a culture of Bacillus subtilis temperature-sensitive mutants growing in a rich medium. At intervals during this replication, samples of cells were transferred to a poor medium to induce sporulation. The results show that the capacity of B. subtilis for induced sporulation reaches a peak about 15 min after chromosome replication has begun. This capacity then declines rapidly, but can be restored by initiating a new round of deoxyribonucleic acid replication. The possibility is discussed that sporulation can be induced only when the chromosome replication fork is passing through a stage 0 operon and that this may be located in the cysA-sul(R) region of the chromosome.
D001412:Bacillus subtilis / Q000254:growth & development* / Q000378:metabolism; D002876:Chromosomes, Bacterial / Q000378:metabolism*; D003470:Culture Media; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis; D005971:Glutamates / Q000378:metabolism; D009154:Mutation; D009876:Operon; D013171:Spores, Bacterial / Q000254:growth & development / Q000378:metabolism; D013696:Temperature; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014364:Tryptophan / Q000378:metabolism
D003470:Culture Media; D004269:DNA, Bacterial; D005971:Glutamates; D014364:Tryptophan; D013936:Thymidine