Immunogenicity of purified, inactivated chikungunya virus in monkeys.
Chikungunya (CHIK) virus, harvested from infected BHK-21 cell cultures and highly purified by a method combining zinc acetate precipitation, Sephadex-Sepharose column chromatography, and sucrose density gradient centrifugation, was subjected to ultraviolet (UV) irradiation and treatment with formalin. Inactivation of the virus by UV light was apparently a first-order reaction. The virus treated with 0.05% formalin at 4 degrees C was inactivated completely after 7 weeks, but when treated with 0.025% formalin retained its plaque infectivity at least 17 weeks. Both UV- and formalin-inactivated viruses induced production of anti-CHIK neutralizing antibodies in Japanese monkeys with no preimmune antibodies. Judging by the titres of antibodies produced, the immunogenic effect of the UV-irradiated virus was superior to that of the formalin-treated virus.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000917:Antibody Formation; D002646:Chikungunya virus / Q000187:drug effects / Q000276:immunology* / Q000302:isolation & purification / Q000528:radiation effects; D005557:Formaldehyde / Q000494:pharmacology; D000882:Haplorhini; D008251:Macaca; D009500:Neutralization Tests; D011830:Radiation Effects; D014466:Ultraviolet Rays; D014613:Vaccines, Attenuated
D000914:Antibodies, Viral; D014613:Vaccines, Attenuated; D005557:Formaldehyde