The use of iodinated RNA for gene localization.
Carrier-free iodination of 5S RNA with (125)I yields a probe suitable for use in cytological RNA-DNA hybridization studies. The genes coding for 5S ribosomal RNA in Drosophila could be localized by autoradiography after a 2-day exposure, whereas a 2-month exposure is needed when the best available [(3)H]RNA is used. The procedure introduces one covalently bound (125)I atom per 100 nucleotides, resulting in a specific activity of over 10(8) dpm/mug of RNA. The method may be readily applied to other problems involving molecular hybridization techniques.
D000818:Animals; D001345:Autoradiography; D002848:Chromatography, DEAE-Cellulose; D002874:Chromosome Mapping; D004247:DNA / Q000302:isolation & purification; D004331:Drosophila melanogaster; D007456:Iodine Isotopes; D007553:Isotope Labeling; D008722:Methods; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000302:isolation & purification; D012469:Salivary Glands / Q000166:cytology; D014316:Tritium
D007456:Iodine Isotopes; D014316:Tritium; D012313:RNA; D004247:DNA