Isolation and properties of Bacillus subtilis strains lysogenized by a clear plaque mutant of bacteriophage phi 105.
A clear plaque mutant of the temperate Bacillus phage phi105 lysogenized a small fraction of infected cells forming an integrated prophage at or near the normal phi105 insertion site. These lysogens exhibited a spontaneous induction rate approximately 1,000-fold lower than wild type and were noninducible (ind(-)) by mitomycin C. Prophage was induced, however, when competent cultures were incubated with transforming DNA. The ind(-) phenotype could not be attributed solely to the clear plaque mutation and appears to involve a cell-specific factor. Lysogenization by the clear plaque mutant, in contrast to wild-type phage, did not cause a marked reduction in transformation efficiency.
D001412:Bacillus subtilis / Q000302:isolation & purification; D001435:Bacteriophages / Q000254:growth & development*; D002874:Chromosome Mapping; D004267:DNA Viruses / Q000254:growth & development*; D004269:DNA, Bacterial; D005809:Genes, Regulator; D008242:Lysogeny; D008937:Mitomycins / Q000494:pharmacology; D009154:Mutation; D011995:Recombination, Genetic; D013696:Temperature; D014170:Transformation, Genetic; D014779:Virus Replication
D004269:DNA, Bacterial; D008937:Mitomycins