Replication of bacteriophage SH-133 in the facultative autotroph Hydrogenomonas facilis. I. Bacteriophage synthesis under heterotrophic, autotrophic, and mixotrophic growth conditions.
The ability of bacteriophage SH-133 to replicate in heterotrophically (H-) and autotrophically (A-) grown Hydrogenomonas facilis was examined. Both the synthesis of infectious phage particles and the efficiency of plating (EOP) were reduced by 90% in A-grown cells. Adsorption of phage and lethal effects on H. facilis were identical in both systems. One-step growth experiments showed that cell lysis preceded the appearance of infectious particles in A-grown cells. Burst size studies with mixotrophically grown cells did not indicate the presence of an inhibitor of phage synthesis indigenous to autotrophic metabolism. DNA synthesis was identical in H- and A-grown infected cells; however, protein synthesis was significantly reduced in A-grown infected cells when compared with protein synthesis in H-grown infected cells. The data suggest that the reduction in EOP and phage synthesis in A-grown cells is caused by a defect in viral protein synthesis which results in the limited production of an essential viral protein at the time of cell lysis.
D000327:Adsorption; D001329:Autolysis; D001426:Bacterial Proteins / Q000096:biosynthesis; D001435:Bacteriophages / Q000378:metabolism; D002244:Carbon / Q000494:pharmacology; D003470:Culture Media; D004269:DNA, Bacterial / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D011549:Pseudomonas / Q000254:growth & development* / Q000378:metabolism; D012329:RNA, Bacterial / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D013997:Time Factors; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication / Q000187:drug effects*
D001426:Bacterial Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D004279:DNA, Viral; D012329:RNA, Bacterial; D012367:RNA, Viral; D014764:Viral Proteins; D002244:Carbon