Use of the single radial diffusion technique for influenza antibody surveys.
A survey of antibody to the influenza A/Hong Kong/1/68 (H3N2) and the variant A/England/42/72 (H3N2) was carried out by two methods, haemagglutination-inhibition (HI) and single radial diffusion (SRD). A comparison of the sensitivity of the two tests indicated that the proportion of sera that produced a zone of 2.5 mm or larger in the SRD tests was equivalent to the proportion that gave HI titres of 1: 40 or more. The test was carried out on serum samples collected from various regions of England. The results showed that approximately 70% had antibody to the A/HK/1/68 virus and only 35% had antibody to A/England/42/72. The SRD test was shown to have several practical advantages for seroepidemiological studies.
D000914:Antibodies, Viral / Q000032:analysis*; D004739:England; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D005779:Immunodiffusion; D007251:Influenza, Human / Q000276:immunology*; D009975:Orthomyxoviridae / Q000276:immunology*
D000914:Antibodies, Viral