Relation of detergent HLB number to solubilization and stabilization of D-alanine carboxypeptidase from Bacillus subtilis membranes.
The ability of various nonionic detergents to solubilize D-alanine carboxypeptidase and other membrane-bound enzymes was correlated to a physical property of the detergent, the HLB number. Only a fairly narrow range of detergents were effective solubilizing agents. Purified carboxypeptidase required either a detergent or detergent plus a lipid fraction for stability. Only those detergents effective in solubilizing the enzyme were effective in stabilizing it.
D000409:Alanine; D001412:Bacillus subtilis / Q000166:cytology / Q000201:enzymology*; D002268:Carboxypeptidases; D002462:Cell Membrane / Q000201:enzymology; D003902:Detergents; D008055:Lipids; D012995:Solubility; D012997:Solvents; D013501:Surface-Active Agents
D003902:Detergents; D008055:Lipids; D012997:Solvents; D013501:Surface-Active Agents; D002268:Carboxypeptidases; D000409:Alanine