NH2-terminal amino acid distribution and amino acid composition of Streptococcus faecalis R soluble and ribosomal proteins.
The NH(2)-terminal amino acid distribution of Streptococcus faecalis R soluble and ribosomal proteins isolated from cells at different stages of growth on either folate-sufficient or folate-deficient medium was determined by the dinitrophenyl method. The NH(2)-terminal residues do not follow the random distribution observed for the total amino acid composition of S. faecalis soluble and ribosomal proteins. Methionine and alanine occur most frequently; serine, threonine, aspartic and glutamic acids, and glycine are also present at the NH(2)-terminal position of S. faecalis R proteins. The absence of folic acid yields cells that are incapable of formylating methionyl-transfer ribonucelic acid tRNA(f) (Met), but does not affect either the qualitative or quantitative NH(2)-terminal distribution of total soluble or total ribosomal proteins compared to cells grown with folate. A small quantitative difference was observed in the frequency of distribution of certain amino acids at the NH(2)-termini between log and stationary phase soluble proteins. The amino acid residues found at the NH(2)-terminal position of S. faecalis proteins are qualitatively similar to those reported for several other organisms.
D000409:Alanine / Q000032:analysis; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis*; D001224:Aspartic Acid / Q000032:analysis; D001322:Autoanalysis; D001426:Bacterial Proteins / Q000032:analysis*; D002474:Cell-Free System; D003470:Culture Media; D004140:Dinitrophenols; D013293:Enterococcus faecalis / Q000032:analysis* / Q000254:growth & development / Q000378:metabolism; D005492:Folic Acid / Q000378:metabolism; D005971:Glutamates / Q000032:analysis; D005998:Glycine / Q000032:analysis; D008715:Methionine / Q000032:analysis; D012270:Ribosomes / Q000032:analysis*; D012694:Serine / Q000032:analysis; D012995:Solubility; D013912:Threonine / Q000032:analysis
D000596:Amino Acids; D001426:Bacterial Proteins; D003470:Culture Media; D004140:Dinitrophenols; D005971:Glutamates; D013912:Threonine; D001224:Aspartic Acid; D012694:Serine; D005492:Folic Acid; D008715:Methionine; D000409:Alanine; D005998:Glycine