Mutant of Bacillus subtilis with a temperature-sensitive autolytic amidase.
A procedure incorporating a petri plate assay for autolysins has been developed for the systematic isolation of mutants with a temperature-sensitive autolytic amidase. We suggest maa as the name for the gene for this enzyme. One such mutant has been characterized as having a heat-sensitive amidase in a variety of studies with both crude autolysin preparations and purified amidase solutions.
D000409:Alanine; D000581:Amidohydrolases / Q000302:isolation & purification / Q000378:metabolism*; D001329:Autolysis; D001412:Bacillus subtilis / Q000201:enzymology* / Q000254:growth & development; D002473:Cell Wall / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D002712:Chlorides; D005796:Genes; D005959:Glucosidases / Q000302:isolation & purification / Q000378:metabolism; D006358:Hot Temperature; D008094:Lithium; D008837:Micrococcus; D009153:Mutagens; D009154:Mutation; D009604:Nitrosoguanidines; D011402:Pronase; D012967:Sodium Dodecyl Sulfate; D013696:Temperature
D002712:Chlorides; D009153:Mutagens; D009604:Nitrosoguanidines; D012967:Sodium Dodecyl Sulfate; D008094:Lithium; D005959:Glucosidases; D011402:Pronase; D000581:Amidohydrolases; D000409:Alanine