Use of microculture plates and the multiple automated sample harvester for in vitro microassay of bacterial toxins.
An in vitro cytotoxicity microassay for the measurement of nanogram quantities of Pseudomonas aeruginosa exotoxin and Vibrio cholerae enterotoxin is described.
D000818:Animals; D001681:Biological Assay; D046508:Culture Techniques / Q000295:instrumentation; D003601:Cytotoxicity Tests, Immunologic; D004768:Enterotoxins / Q000032:analysis*; D005069:Evaluation Studies as Topic; D006367:HeLa Cells; D006801:Humans; D007739:L Cells; D008722:Methods; D051379:Mice; D011550:Pseudomonas aeruginosa / Q000032:analysis*; D013936:Thymidine / Q000378:metabolism; D014118:Toxins, Biological / Q000032:analysis*; D014316:Tritium; D014734:Vibrio cholerae / Q000032:analysis*
D004768:Enterotoxins; D014118:Toxins, Biological; D014316:Tritium; D013936:Thymidine