The bacterial degradation of flavonoids. Oxidative fission of the A-ring of dihydrogossypetin by a Pseudomonas sp.
Cell-free extracts prepared from a Pseudomonas sp., grown on (+)-catechin, oxidized dihydrogossypetin (3',4',5,7,8-pentahydroxyflavanonol) by cleaving the A-ring to form oxaloacetic acid from C-5, C-6, C-7 and C-8 together with 5-(3,4-dihydroxyphenyl)-4-hydroxy-3-oxovalero-delta-lactone. The structure of this lactone was confirmed by synthesis of related phenylvalerolactones.
D002247:Carbon Isotopes; D002474:Cell-Free System; D002855:Chromatography, Thin Layer; D004492:Edetic Acid; D005419:Flavonoids / Q000378:metabolism*; D007783:Lactones / Q000096:biosynthesis / Q000138:chemical synthesis; D013058:Mass Spectrometry; D010071:Oxaloacetates / Q000096:biosynthesis; D010084:Oxidation-Reduction; D011549:Pseudomonas / Q000378:metabolism*; D011773:Pyruvates / Q000032:analysis; D012988:Soil Microbiology; D013053:Spectrophotometry
D002247:Carbon Isotopes; D005419:Flavonoids; D007783:Lactones; D010071:Oxaloacetates; D011773:Pyruvates; D004492:Edetic Acid