Regulation of the  -methylgalactoside transport system and the galatose-binding protein by the cell cycle of Escherichia coli.
The synthesis of the periplasmic galactose-binding protein of E. coli is regulated by events occurring during its cell cycle, and proceeds in synchronized cells for only a short period after cell division is completed. Transport activity mediated by the beta-methylgalactoside transport system follows closely the synthesis pattern of the binding protein.A mutant, E. coli BUG-6, exhibits temperature-sensitive cell division [Reeve et al. (1970) J. Bacteriol. 104, 1052-1064], synthesizing galactose-binding protein at the permissive but not at the nonpermissive temperature. Galactose-binding protein synthesized at the permissive temperature is not degraded after the culture is shifted to the nonpermissive temperature. Polyacrylamide gel electrophoresis of the periplasmic proteins of BUG-6 grown at the permissive and nonpermissive temperatures suggests that several, but not all, periplasmic proteins are subject to the same regulatory control by the cell cycle as the galactose-binding protein.
D000409:Alanine / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D001345:Autoradiography; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D002247:Carbon Isotopes; D002455:Cell Division; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism*; D005696:Galactosidases / Q000378:metabolism; D005779:Immunodiffusion; D007785:Lactose / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D009154:Mutation; D011392:Proline / Q000378:metabolism; D011485:Protein Binding; D013696:Temperature; D014161:Transduction, Genetic
D001426:Bacterial Proteins; D002247:Carbon Isotopes; D003470:Culture Media; D011392:Proline; D000469:Alkaline Phosphatase; D005696:Galactosidases; D007930:Leucine; D007785:Lactose; D000409:Alanine; D005690:Galactose