Intracellular and extracellular degranulation of human polymorphonuclear azurophil and specific granules induced by immune complexes.
The nature of the phagocytic stimulus determines the degree of degranulation. Specifically, immune complexes induced more degranulation than did antigen or antibody alone. The release of constituents from the two granule classes represented independent phenomena. Relatively more of the specific granule proteins were released into the medium during phagocytosis, whereas more of the azurophil proteins were associated with the phagosomes.
D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D000936:Antigen-Antibody Complex; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D003168:Complement Fixation Tests; D003594:Cytoplasmic Granules / Q000201:enzymology / Q000276:immunology*; D005966:Glucuronidase / Q000097:blood; D006801:Humans; D007106:Immune Sera; D005779:Immunodiffusion; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007840:Latex; D008247:Lysosomes; D008863:Microspheres; D009504:Neutrophils / Q000276:immunology*; D010447:Peptide Hydrolases / Q000032:analysis; D010544:Peroxidases / Q000097:blood; D010587:Phagocytosis; D011817:Rabbits / Q000276:immunology; D012710:Serum Albumin, Bovine
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D007840:Latex; D012710:Serum Albumin, Bovine; D007770:L-Lactate Dehydrogenase; D010544:Peroxidases; D000469:Alkaline Phosphatase; D005966:Glucuronidase; D010447:Peptide Hydrolases