Repeated DNA sequences in the heterochromatic Y chromosome of adult Drosophila melanogaster.
DNA isolated from Drosophila melanogaster adults (XX, XY, XXY, XYY) was used in DNA-DNA hybridization experiments on nitrocellulose filters. Filter-bound DNA of "high-heterochromatin" flies (those with one or more Y chromosomes) is more effective in forming hybrid duplexes than is XX DNA. The quantitative difference in hybridization efficiency is due primarily to molecules with a relatively high thermal stability (duplexes that dissociate in the temperature range 80-90 degrees ). Hybridization experiments with DNA samples that have been fractionated into reiterated and unique portions show that the majority of the hybrid duplexes formed involve reiterated DNA. A small, but highly specific, interaction of unique DNA sequences has also been detected in our experiments. These data indicate that a class of repeated DNA sequences is associated with the constitutive heterochromatin, specifically with the Y chromosome of D. melanogaster. Evidence is presented that this same class of molecules, or very similar ones, is to be found in other portions of the genome, presumably in X-chromosomal or centromeric heterochromatin.
D000818:Animals; D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D004331:Drosophila melanogaster; D005260:Female; D006570:Heterochromatin / Q000032:analysis*; D006358:Hot Temperature; D007621:Karyotyping; D008297:Male; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D012730:Sex Chromosomes / Q000032:analysis*; D014316:Tritium
D006570:Heterochromatin; D014316:Tritium; D004247:DNA