Use of affinity chromatography for the quantitative study of acceptor-ligand interactions: The lactose synthetase system.
From the effects of N-acetyl-d-glucosamine and d-glucose on the elution of the A protein of human lactose synthetase from a column of Sepharose-alpha-lactalbumin, values of 200m(-1) and 0.57m(-1) are deduced for the association constants describing the interaction between the enzyme and the respective monosaccharides.
D000081:Acetamides; D001665:Binding Sites; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D005260:Female; D005690:Galactose; D005944:Glucosamine; D005947:Glucose / Q000032:analysis; D006801:Humans; D007700:Kinetics; D007768:Lactalbumin; D007788:Lactose Synthase / Q000032:analysis*; D008024:Ligands
D000081:Acetamides; D008024:Ligands; D007768:Lactalbumin; D007788:Lactose Synthase; D005947:Glucose; D005944:Glucosamine; D005690:Galactose