Reactivity of purified proteins and polysaccharides from Mycobacterium tuberculosis in delayed skin test and cultured lymphocyte mitogenesis assays.
Hypersensitivity responses to two highly purified and well characterized mycobacterial proteins and a preparation of mycobacterial arabinogalactanarabinomannan were studied by using guinea pig skin tests and in vitro-cultured human lymphocyte mitogenesis stimulation assays. The purified proteins were found to elicit delayed-type hypersensitivity skin test reactions and to stimulate mitogenesis in cultured lymphocytes. The polysaccharide preparation was found to evoke delayed skin tests but not to stimulate cultured lymphocytes.
D000818:Animals; D000942:Antigens, Bacterial; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D008938:Mitosis; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification*; D012882:Skin Tests; D013936:Thymidine / Q000378:metabolism; D014373:Tuberculin; D014374:Tuberculin Test
D000942:Antigens, Bacterial; D011135:Polysaccharides, Bacterial; D014373:Tuberculin; D013936:Thymidine