Dihydrofolate reductase: low-resolution mass-spectrometric analysis of an elastase digest as a sequencing tool.
An elastase digest of a protein of unknown structure, dihydrofolate reductase, was studied by mass spectrometry. This soluble digest contained a large number of small peptides in different yields, within the ideal molecular-weight range (200-1200) for mixture-analysis mass spectrometry. Sequences of the major component peptides in the digest are reported.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002852:Chromatography, Ion Exchange; D004590:Electrophoresis, Paper; D005612:Freeze Drying; D007780:Lacticaseibacillus casei / Q000201:enzymology; D013058:Mass Spectrometry; D008970:Molecular Weight; D010196:Pancreatic Elastase; D010446:Peptide Fragments / Q000032:analysis; D012995:Solubility; D013762:Tetrahydrofolate Dehydrogenase
D000596:Amino Acids; D010446:Peptide Fragments; D013762:Tetrahydrofolate Dehydrogenase; D010196:Pancreatic Elastase