The use of the hamster stomach in vitro as an assay preparation for prostaglandins.
1. Hamster stomach strips in vitro are useful test preparations for the assay of prostaglandins E and F in the nanogram concentration range. Three-minute cycles can be used for long periods without spontaneous contractions or significant base-line instability.2. The mean percentage error and the precision index of six 4-point assays of prostaglandin E(2) were 7.8 and 0.057, respectively.3. The relative sensitivity to different prostaglandins was E(2)/E(1) approximately 1, E(2)/F(1alpha) approximately 65 and E(2)/A(1) approximately 400.4. These preparations were relatively insensitive to 5-hydroxytryptamine (prostaglandin E(2)/5-HT approximately 20,000) and histamine (prostaglandin E(2)/histamine approximately 200).5. These results suggest that the hamster stomach preparation can be used to differentiate between 5-hydroxytryptamine and prostaglandins.
D000818:Animals; D001681:Biological Assay; D006224:Cricetinae; D006632:Histamine / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D008722:Methods; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D010477:Perfusion; D011448:Prostaglandin Antagonists; D011453:Prostaglandins / Q000494:pharmacology*; D012701:Serotonin / Q000494:pharmacology; D013270:Stomach / Q000187:drug effects*
D011448:Prostaglandin Antagonists; D011453:Prostaglandins; D012701:Serotonin; D006632:Histamine