Metabolism of Richettsia tsutsugamushi and Rickettsia rickettsi in irradiated host cells.
The metabolism of Rickettsia tsutsugamushi (Gilliam strain) multiplying in irradiated L cells was investigated by methods involving the use of (14)C-labeled substrates and cycloheximide, an inhibitor of eukaryotic metabolism. Cycloheximide-resistant amino acid and adenine incorporations were appreciably higher in infected than in uninfected cultures during the period from 3 to 5 or 6 days postinoculation. The metabolism of R. rickettsi was similarly studied in primary duck embryo cells, which are more susceptible to infection with this rickettsia than are L cells. A difference in cycloheximide-resistant activity between infected and uninfected cultures was also noted, but was small. This finding is attributed to the more limited growth of R. rickettsi.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002247:Carbon Isotopes; D003513:Cycloheximide; D004372:Ducks; D007739:L Cells / Q000528:radiation effects*; D012285:Orientia tsutsugamushi / Q000378:metabolism* / Q000528:radiation effects; D011830:Radiation Effects; D012284:Rickettsia rickettsii / Q000378:metabolism* / Q000528:radiation effects
D000596:Amino Acids; D002247:Carbon Isotopes; D003513:Cycloheximide