Aldosterone-induced protein in toad urinary bladder.
Simultaneous electrophysiological and biochemical experiments demonstrated a specific aldosterone-induced protein in paired urinary hemibladders isolated from the toad Bufo marinus. Whenever aldosterone stinlmulated short-circuit current, aldosterone specifically increased [(35)S] methionine incorporation into a low-molecular-weight protein (about 12,000). Comparative studies with dexamethasone and insulin and inhibitory studies with spironolactone and actinomycin D suggest mineralocorticoid specificity.
D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D001693:Biological Transport, Active; D002024:Bufo marinus; D003609:Dactinomycin / Q000494:pharmacology; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D007700:Kinetics; D008564:Membrane Potentials; D008715:Methionine / Q000378:metabolism; D000451:Mineralocorticoid Receptor Antagonists; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D014176:Protein Biosynthesis; D012964:Sodium / Q000378:metabolism; D013148:Spironolactone / Q000494:pharmacology; D013268:Stimulation, Chemical; D013462:Sulfur Radioisotopes; D001743:Urinary Bladder / Q000378:metabolism* / Q000502:physiology
D007328:Insulin; D000451:Mineralocorticoid Receptor Antagonists; D013462:Sulfur Radioisotopes; D003609:Dactinomycin; D013148:Spironolactone; D000450:Aldosterone; D012964:Sodium; D008715:Methionine