Mild and efficient solubilization of Escherichia coli cells for the analysis of chromosomal DNA by sucrose gradient centrifugation.
D000596:Amino Acids / Q000378:metabolism; D002455:Cell Division; D002499:Centrifugation, Density Gradient; D002876:Chromosomes, Bacterial / Q000032:analysis*; D004269:DNA, Bacterial / Q000032:analysis*; D004492:Edetic Acid; D004926:Escherichia coli / Q000032:analysis* / Q000166:cytology / Q000378:metabolism; D000431:Ethanol; D005069:Evaluation Studies as Topic; D008722:Methods; D009113:Muramidase; D012967:Sodium Dodecyl Sulfate; D012995:Solubility; D013941:Thymine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium
D000596:Amino Acids; D004269:DNA, Bacterial; D014316:Tritium; D012967:Sodium Dodecyl Sulfate; D000431:Ethanol; D004492:Edetic Acid; D009113:Muramidase; D013941:Thymine