Characterization of virus-specific RNAs from subacute sclerosing panencephalitis virus-infected CV-1 cells.
CV-1 cells infected with subacute sclerosing panencephalitis (SSPE) virus incorporated uridine-(3)H into at least four virus-specific RNA components in the presence of actinomycin D. The component sedimenting fastest had a sedimentation coefficient of 50s corresponding to a molecular weight of 6 x 10(6). The other three RNA components have sedimentation constants of 35s, 22s, and 18s corresponding to molecular weights of 2.5 x 10(6), 1.0 x 10(6), and 0.75 x 10(6), respectively. The base composition of the 50s RNA is distinct from that of cellular RNA and comparable with base compositions of viral RNAs of other paramyxoviruses. The base composition of the 18s RNA shows approximate complementarity with the 50s RNA. RNA-RNA annealing experiments using unlabeled 50s SSPE RNA with labeled 18s RNA from cells infected with SSPE virus or measles virus show 100% annealing with 18s SSPE RNA but only 60% annealing with 18s measles RNA. These experiments suggest some differences between the 18s RNAs of SSPE virus-infected cells and measles virus-infected cells.
D000225:Adenine / Q000032:analysis; D000818:Animals; D002250:Carbon Radioisotopes; D002460:Cell Line; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D002586:Cesium; D003596:Cytosine / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004668:Encephalitis Viruses / Q000032:analysis* / Q000302:isolation & purification; D006147:Guanine / Q000032:analysis; D000882:Haplorhini; D007668:Kidney; D008459:Measles virus / Q000032:analysis; D009693:Nucleic Acid Hybridization; D010761:Phosphorus Radioisotopes; D012335:RNA, Ribosomal / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D013344:Subacute Sclerosing Panencephalitis / Q000382:microbiology*; D013395:Sucrose; D013997:Time Factors; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D002250:Carbon Radioisotopes; D010761:Phosphorus Radioisotopes; D012335:RNA, Ribosomal; D012367:RNA, Viral; D014316:Tritium; D002586:Cesium; D013395:Sucrose; D006147:Guanine; D003596:Cytosine; D000225:Adenine; D014529:Uridine