The occurrence of two types of synthesis of deoxyribonucleic acid during normal growth in Bacillus subtilis.
A study of the relative utilization of thymine and thymidine as precursors for DNA synthesis during normal growth in Bacillus subtilis showed that thymine serves preferentially as a precursor for ;repair' synthesis, whereas thymidine is used preferentially for ;replicative' synthesis. Further, evidence was obtained which suggests that during normal growth both ;replicative' and ;repair' DNA syntheses occur simultaneously. ;Repair' synthesis is distinguished not only on the basis of its preferential utilization of thymine but also by its selective inhibition by caffeine. ;Replicative' synthesis, however, is selectively inhibited by 6-(p-hydroxyphenylazo)-uracil. ;Repair' synthesis would seem to be a ;pre-fork' phenomenon and its inhibition is highly lethal to the cell.
D001391:Azo Compounds; D001412:Bacillus subtilis / Q000378:metabolism*; D002110:Caffeine / Q000494:pharmacology; D002250:Carbon Radioisotopes; D002499:Centrifugation, Density Gradient; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis*; D010636:Phenols; D013936:Thymidine / Q000378:metabolism; D013941:Thymine / Q000378:metabolism; D014316:Tritium; D014498:Uracil / Q000494:pharmacology; D014529:Uridine / Q000378:metabolism
D001391:Azo Compounds; D002250:Carbon Radioisotopes; D004269:DNA, Bacterial; D010636:Phenols; D014316:Tritium; D002110:Caffeine; D014498:Uracil; D013941:Thymine; D013936:Thymidine; D014529:Uridine