A low-molecular-weight protein cross-reacting with human liver N-acetyl-beta-D-glucosaminidase.
Antisera were raised to preparations of hexosaminidase isoenzymes A and B purified from human liver. Protein that cross-reacted with the liver hexosaminidase was detected by an antibody-consumption method. A cross-reacting protein with a low molecular weight (20000) was partially characterized and purified from control human liver. This protein is also present in the liver of patients with Tay-Sachs disease or with Sandhoff's disease. Hexosaminidases A and B gave an immunological reaction of partial identity with the low-molecular-weight protein. The possible identity of the low-molecular-weight cross-reacting protein as a subunit of hexosaminidase is discussed.
D000081:Acetamides; D000818:Animals; D000937:Antigen-Antibody Reactions; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003429:Cross Reactions; D004589:Electrophoresis, Disc; D005959:Glucosidases / Q000378:metabolism; D006596:Hexosaminidases; D006801:Humans; D007106:Immune Sera / Q000302:isolation & purification*; D005779:Immunodiffusion; D007527:Isoenzymes; D008064:Lipidoses / Q000276:immunology; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D009113:Muramidase; D011817:Rabbits / Q000276:immunology
D000081:Acetamides; D007106:Immune Sera; D007527:Isoenzymes; D005959:Glucosidases; D006596:Hexosaminidases; D009113:Muramidase