Mutant of Bacillus subtilis lacking exo-beta-N-acetylglucosaminidase activity.
A procedure for the isolation of exo-beta-N-acetylglucosaminidase mutants, by using a plate assay method incorporating a fluorescent substrate, has been developed. A mutant lacking exo-beta-N-acetylglucosaminidase activity has been isolated and shown to grow, divide, autolyze, and sporulate as well as the parental strain.
D001329:Autolysis; D001412:Bacillus subtilis / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D003470:Culture Media; D005470:Fluorometry; D005944:Glucosamine; D005971:Glutamates; D006596:Hexosaminidases / Q000096:biosynthesis* / Q000378:metabolism; D009154:Mutation; D013171:Spores, Bacterial / Q000254:growth & development
D003470:Culture Media; D005971:Glutamates; D006596:Hexosaminidases; D005944:Glucosamine