Phenotype recognition of pyocyanine mutants in pseudomonas aeruginosa.
Certain classes of pyocyanine mutants in Pseudomonas aeruginosa escape detection when screened in the presence of wild-type or other mutant cells. A technique is described for recognizing mutant phenotypes after cells are in individual agar wells. The procedure eliminates cross-feeding phenomena as well as the masking of mutant phenotypes by diffusing pyocyanine produced by nearby clones.
D000362:Agar; D001431:Bacteriological Techniques; D009154:Mutation; D010641:Phenotype; D010860:Pigments, Biological / Q000096:biosynthesis*; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*
D010860:Pigments, Biological; D000362:Agar