An electron-capture gas-liquid-chromatographic method for the determination of prostaglandin F2alpha in biological fluids.
A sensitive electron-capture gas-liquid-chromatographic method for the determination of sub-nanogram quantities of prostaglandin F(2alpha) was developed. The method is based on the sub-microgram scale conversion of the prostaglandin into the electron-capturing pentafluorobenzyl ester, and analysis of the latter as the tris-trimethylsilyl ether. The lower limit of detection was 12.5pg of the ester injected ;on-column' as the silylated product. The method was successfully applied to the determination of prostaglandin F(2alpha) in monkey plasma. The specificity of the analytical procedure was increased by incorporating a thin-layer chromatographic fractionation before gas-liquid chromatography. The utility of the analytical methodology developed was demonstrated by its application to the determination of plasma concentrations of intact prostaglandin F(2alpha) in a Rhesus monkey, after subcutaneous administration of a single dose of prostaglandin F(2alpha). The electron-capture gas-liquid-chromatographic assay is compared with the radioimmunoassay and the gas-liquid-chromatographic-mass-spectrometry assay for the determination of prostaglandin F(2alpha).
D000818:Animals; D002849:Chromatography, Gas / Q000379:methods; D002855:Chromatography, Thin Layer; D004583:Electrons; D005260:Female; D005464:Fluorobenzenes; D000882:Haplorhini; D007279:Injections, Subcutaneous; D013058:Mass Spectrometry; D011453:Prostaglandins / Q000032:analysis* / Q000097:blood; D011863:Radioimmunoassay; D014316:Tritium
D005464:Fluorobenzenes; D011453:Prostaglandins; D014316:Tritium