Reovirus-directed ribonucleic acid synthesis in infected L cells.
Reovirus replication in L-929 mouse fibroblasts was unaffected by 0.5 mug of actinomycin per ml, a concentration which inhibited cell ribonucleic acid (RNA) synthesis by more than 90%. Under these conditions of selective inhibition, the formation of both single-stranded and double-stranded virus-specific RNA was detected beginning at 6 hr after infection. The purified double-stranded RNA was similar in size and base composition to virus RNA and presumably was incorporated into mature virus. The single-stranded RNA formed ribonuclease-resistant duplexes when annealed with denatured virus RNA but did not self-anneal, thus indicating that it includes copies of only one strand of the duplex. The single-stranded RNA was polyribosome-associated and may function as the virus messenger RNA. Production of both types of virus-induced RNA required protein synthesis 6 to 9 hr after infection. At later times in the infectious cycle, only double-stranded RNA synthesis was dependent on continued protein formation.
D000818:Animals; D002247:Carbon Isotopes; D002498:Centrifugation; D002499:Centrifugation, Density Gradient; D002845:Chromatography; D046508:Culture Techniques; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D007739:L Cells; D010759:Phosphorus Isotopes; D012316:RNA Nucleotidyltransferases; D012367:RNA, Viral / Q000096:biosynthesis* / Q000302:isolation & purification; D012087:Reoviridae; D012270:Ribosomes; D014529:Uridine; D014764:Viral Proteins / Q000096:biosynthesis; D014776:Virus Cultivation; D014779:Virus Replication
D002247:Carbon Isotopes; D010759:Phosphorus Isotopes; D012367:RNA, Viral; D014764:Viral Proteins; D003609:Dactinomycin; D003513:Cycloheximide; D012316:RNA Nucleotidyltransferases; D014529:Uridine