In vitro synthesis of adenovirus core proteins.
mRNA extracted from polysomes of KB cells at late stages of productive infection with adenovirus type 2 was translated in a cell-free system derived from Krebs II ascites cells. Two of the polypeptides obtained in this reaction corresponded to the adenovirus core protein V and the precursor to core protein VII. The following criteria were used to establish identity between the in vitro products and the virion proteins: comigration during sodium dodecyl sulfatepolyacrylamide gel electrophoresis, cochromatography in sodium dodecyl sulfate-hydroxyapatite, specific immunoprecipitation of the precursor to core protein VII, and tryptic peptide analysis.
D000256:Adenoviridae / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D002277:Carcinoma; D002287:Carcinoma, Krebs 2; D002460:Cell Line; D002474:Cell-Free System; D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006882:Hydroxyapatites; D005779:Immunodiffusion; D009062:Mouth Neoplasms; D010452:Peptide Biosynthesis; D010455:Peptides / Q000032:analysis; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000378:metabolism; D013462:Sulfur Radioisotopes; D014316:Tritium; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis*; D014776:Virus Cultivation
D006882:Hydroxyapatites; D010455:Peptides; D011498:Protein Precursors; D012334:RNA, Neoplasm; D013462:Sulfur Radioisotopes; D014764:Viral Proteins; D014316:Tritium