Pancreatic ribonuclease: enzymic and physiological properties of a cross-linked dimer.
Monomeric ribonuclease A has very low activity toward typically double-stranded RNA's; the dimeric form of ribonuclease A obtained by cross linking the enzyme by dimethyl suberimidate has more than 78 times the activity of the monomer toward polyadenylate . polyuridylate and 440 times the activity of the monomer toward the double-stranded RNA of a virus from Penicillium chrysogenum. The half-life of the dimer in the bloodstream of the rat is 12 times that of the mononmer.
D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D004586:Electrophoresis; D006207:Half-Life; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D010179:Pancreas / Q000201:enzymology*; D010408:Penicillium chrysogenum; D011063:Poly A-U; D011108:Polymers; D011487:Protein Conformation; D012367:RNA, Viral; D012260:Ribonucleases / Q000097:blood; D012967:Sodium Dodecyl Sulfate; D013329:Structure-Activity Relationship
D011108:Polymers; D012367:RNA, Viral; D011063:Poly A-U; D012967:Sodium Dodecyl Sulfate; D012260:Ribonucleases