Enzyme activity and composition of myelin and subcellular fractions in the developing rat brain.
1. Subcellular fractions and myelin were isolated from developing and adult rat brain. 2. Measurements of chemical composition and enzyme activities indicate the presence of a second myelin-like fraction mainly in the brain of developing rats. 3. This membrane fraction has a different lipid composition from myelin, but resembles myelin in its content of phosphohydrolase and aminopeptidase activity. 4. It is suggested that the second myelin-like fraction may be a submicrosomal contaminant or it may be derived from oligodendroglial plasma membrane during myelinogenesis.
D000110:Acetylcholinesterase / Q000032:analysis; D000251:Adenosine Triphosphatases / Q000032:analysis; D000818:Animals; D001921:Brain / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development*; D002802:Cholinesterases / Q000032:analysis; D004950:Esterases / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D008297:Male; D008854:Microscopy, Electron; D008861:Microsomes / Q000032:analysis; D009186:Myelin Sheath / Q000032:analysis*; D009696:Nucleic Acids / Q000032:analysis; D011506:Proteins / Q000032:analysis; D051381:Rats
D005227:Fatty Acids; D009696:Nucleic Acids; D011506:Proteins; D004950:Esterases; D000110:Acetylcholinesterase; D002802:Cholinesterases; D000251:Adenosine Triphosphatases