title: Relative rates of lysis of staphylococcal cell walls by lytic enzymes from various bacteriophage types.
abstract: Lytic enzymes were isolated from 14 strains of phage-infected Staphylococcus aureus. Cell walls were prepared from the same uninfected strains of bacteria. Comparison of the lytic rates was made for each enzyme, with each of the cell walls as substrate. Differences in the rate of substrate utilization of the various cell wall types exceeded 10-fold. Cell walls from strains 42E, 29, and 77 were the best substrates, whereas cell walls from strains 3C, 80, and 187 were the poorest substrates. The cell wall amino acid composition is discussed as related to lytic enzyme specificity. A possible explanation of phage typing of staphylococcal cells, based on enzyme activity and cell wall composition, is presented.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001433:Bacteriolysis; D002473:Cell Wall / Q000032:analysis / Q000187:drug effects; D010447:Peptide Hydrolases; D013210:Staphylococcus; D013204:Staphylococcus Phages / Q000201:enzymology*
chemical_list: D000596:Amino Acids; D010447:Peptide Hydrolases