Tetracycline inhibition of a lipase from Corynebacterium acnes.
A lipase which hydrolyzes triglycerides (tricaprylin and trilaurin) and naphthyl laurate was obtained from the broth of Corynebacterium acnes cultures by ammonium sulfate fractionation. Ca(2+) and sodium taurocholate stimulated activity of the enzyme. Ethylenediaminetetraacetic acid (EDTA) did not inhibit activity of the Ca(2+)-activated enzyme, but lipolytic activity was inhibited by EDTA in the absence of Ca(2+). Tetracycline (10(-4)m) produced a slight inhibition of the lipase activity with 5 x 10(-5)m or less showing no effect on the lipase activity. However, complete inhibition by tetracycline at 10(-4)m was observed for Ca(2+)-activated enzyme. Tetracycline inhibition of the C. acnes lipase could be demonstrated at concentrations as low as 10(-6)m.
D000152:Acne Vulgaris / Q000188:drug therapy; D001647:Bile Acids and Salts / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D011232:Chemical Precipitation; D002855:Chromatography, Thin Layer; D003124:Colorimetry; D003352:Corynebacterium / Q000187:drug effects / Q000201:enzymology*; D004492:Edetic Acid / Q000494:pharmacology; D004789:Enzyme Activation; D006801:Humans; D006868:Hydrolysis; D008049:Lipase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000644:Quaternary Ammonium Compounds; D013431:Sulfates; D013752:Tetracycline / Q000494:pharmacology* / Q000627:therapeutic use; D014280:Triglycerides / Q000378:metabolism
D001647:Bile Acids and Salts; D000644:Quaternary Ammonium Compounds; D013431:Sulfates; D014280:Triglycerides; D004492:Edetic Acid; D008049:Lipase; D013752:Tetracycline; D002118:Calcium