title: Maturation of hemolysin-producing cell clones. I. The kinetics of the induction period of an in vitro hemolysin response to erythrocyte antigen.
abstract: INVESTIGATIONS OF THE INDUCTION PERIOD OF AN IN VITRO HEMOLYSIN RESPONSE TO SHEEP ERYTHROCYTE ANTIGEN REVEALED THE FOLLOWING: 1. After antigen stimulation precursors of plaque-forming cells rapidly maturate to the point of hemolysin production. 2. Initial maturation probably occurs in the absence of cell division. 3. After initial maturation, a latent period of about 12 hr occurs before the first doubling of PFC. 4. At least the first three cell doublings are synchronous, with a generation time of 7-8 hr. 5. Synchronous cell division implies that all precursor cells are at the same point in the cell cyde when they are initially stimulated.
mesh_terms: D000818:Animals; D000937:Antigen-Antibody Reactions; D000941:Antigens; D002455:Cell Division / Q000187:drug effects; D002645:Chickens; D002999:Clone Cells / Q000187:drug effects; D046508:Culture Techniques; D004912:Erythrocytes; D005467:Floxuridine / Q000494:pharmacology; D006460:Hemolysin Proteins; D006736:Horses; D007700:Kinetics; D008297:Male; D051379:Mice; D011691:Puromycin / Q000494:pharmacology; D012756:Sheep; D013154:Spleen / Q000276:immunology; D013997:Time Factors
chemical_list: D000941:Antigens; D006460:Hemolysin Proteins; D005467:Floxuridine; D011691:Puromycin