title: Cell envelope of an iron-oxidizing bacterium: studies of lipopolysaccharide and peptidoglycan.
abstract: Further structural detail is presented of the cell envelope of the chemolithotroph Ferrobacillus ferrooxidans (Thiobacillus ferrooxidans). Thin sections of purified lipopolysaccharide (LPS) and peptidoglycan show structures comparable to those seen in the envelope of intact cells, whereas negative stains of LPS appear as sheets, or ribbons, or both. The sugars common to LPS, namely, heptose, glucose, galactose, mannose, and 2-keto-3-deoxyoctulosonate, were identified. The cations, iron, calcium, and magnesium, were associated with LPS. The purified LPS had a density of 1.28 and an uncorrected sedimentation coefficient of 99.9S.
mesh_terms: D002467:Cell Nucleus; D002473:Cell Wall / Q000032:analysis / Q000378:metabolism; D002849:Chromatography, Gas; D006025:Glycosaminoglycans / Q000378:metabolism; D006595:Hexosamines / Q000032:analysis; D006601:Hexoses / Q000032:analysis; D006651:Histocytochemistry; D007501:Iron / Q000032:analysis; D008070:Lipopolysaccharides / Q000302:isolation & purification / Q000378:metabolism; D008274:Magnesium / Q000032:analysis; D008854:Microscopy, Electron; D009584:Nitrogen / Q000032:analysis; D010758:Phosphorus / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification; D012270:Ribosomes; D013855:Thiobacillus / Q000166:cytology*
chemical_list: D006025:Glycosaminoglycans; D006595:Hexosamines; D006601:Hexoses; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D010758:Phosphorus; D007501:Iron; D008274:Magnesium; D009584:Nitrogen