The outward transport of cortisol by mammalian cells in vitro.
It has been determined that cortisol and a few other steroids are transported outward from certain mammalian cells growing in vitro. The extrusion process is temperature dependent, glucose dependent, saturable, and operates for only a few selected steroids. Many, but not all, steroids are able to block the extrusion process but are not themselves transported. The outward transport process for steroids has been found in mouse fibroblasts, mouse lymphoma cells, and functional mouse adrenal gland tumor cells growing in vitro. The transport process is not present in two varieties of cells cultured from human sources-HeLa or diploid fibroblasts, WI-38.
D000818:Animals; D001693:Biological Transport, Active; D002247:Carbon Isotopes; D002460:Cell Line / Q000187:drug effects / Q000378:metabolism; D002854:Chromatography, Paper; D046508:Culture Techniques; D003687:Dehydroepiandrosterone / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D006367:HeLa Cells / Q000378:metabolism*; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism*; D007739:L Cells; D008168:Lung; D008223:Lymphoma; D051379:Mice; D013256:Steroids / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D002247:Carbon Isotopes; D013256:Steroids; D003687:Dehydroepiandrosterone; D005947:Glucose; D006854:Hydrocortisone