Rapid purification and the immunological specificity of mammalian microtubular paracrystals possessing an ATPase activity.
A new procedure for the rapid purification of vinblastine-induced microtubular paracrystals is described. The paracrystals constitute about 2 per cent of the total protein in L cells. They contain a labile ATPase with a Michaelis constant of 1.0 x 10(-4). In the immunofluorescence test, sera prepared against paracrystals reacted positively with intracellular paracrystals and the mitotic apparatus. Antisera against paracrystals from a malignant murine cell also labeled the mitotic apparatus of HeLa and chicken embryonic cells, revealing the presence of a common or related antigen in the mitotic apparatus of these higher vertebrates.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000372:Agglutination Tests; D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D046508:Culture Techniques; D005347:Fibroblasts / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D007106:Immune Sera; D005779:Immunodiffusion; D007739:L Cells; D008722:Methods; D051379:Mice; D008854:Microscopy, Electron; D008938:Mitosis; D009940:Organoids / Q000032:analysis / Q000187:drug effects / Q000201:enzymology / Q000276:immunology*; D010759:Phosphorus Isotopes; D011506:Proteins / Q000032:analysis; D011817:Rabbits; D013997:Time Factors; D014747:Vinblastine / Q000494:pharmacology; D014750:Vincristine
D007106:Immune Sera; D010759:Phosphorus Isotopes; D011506:Proteins; D014750:Vincristine; D014747:Vinblastine; D000251:Adenosine Triphosphatases