Isolation and properties of chondroitin sulphates from bovine heart valves.
1. Several protein-polysaccharides were isolated from the soluble extracts of bovine heart valves by sedimentation equilibrium in a caesium chloride density gradient (Meyer, Preston & Lowther, 1969). 2. Compositional and structural studies indicated that the polysaccharide moiety was chondroitin sulphate. Differences in the protein content of the products were observed. There was no evidence suggesting the presence of keratan sulphate. 3. Sedimentation studies indicated that the molecular weights of the samples were between 4.2x10(4) and 6.5x10(4). The results are discussed in terms of a basic model for the protein-polysaccharides of two polysaccharide chains linked by a protein of variable size.
D000818:Animals; D002417:Cattle; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D002807:Chondroitin / Q000302:isolation & purification*; D006025:Glycosaminoglycans / Q000032:analysis; D006351:Heart Valves / Q000032:analysis*; D008961:Models, Structural; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis; D013431:Sulfates / Q000302:isolation & purification
D002712:Chlorides; D006025:Glycosaminoglycans; D011506:Proteins; D013431:Sulfates; D002586:Cesium; D002807:Chondroitin