The composition and physicochemical properties of bovine nasal-septa protein-polysaccharide complex.
1. Protein-polysaccharide complexes were prepared in three different ways and the gross stoicheiometry of the complexes compared. 2. The neutral sugar content was ascertained and the possibility of a glycoprotein occurring with chondroitin sulphate and keratosulphate is discussed. 3. Physical data support a molecule of molecular weight 3.2x10(6)-5.8x10(6) with a roughly spherical domain and an average radius of gyration of 1390A. Such a particle is highly solvated. The complex is heavily charged with the sulphate groups on the outside. 4. These findings are discussed in the light of the physiological role of protein-polysaccharide light fraction (PPL) in cartilage.
D000818:Animals; D002356:Cartilage / Q000032:analysis*; D002417:Cattle; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002845:Chromatography; D004586:Electrophoresis; D005643:Fucose / Q000032:analysis; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006025:Glycosaminoglycans / Q000032:analysis*; D006595:Hexosamines / Q000032:analysis; D066298:In Vitro Techniques; D008358:Mannose / Q000032:analysis; D009088:Mucoproteins / Q000032:analysis*; D009300:Nasal Septum / Q000032:analysis*; D014461:Ultracentrifugation; D014783:Viscosity; D014994:Xylose / Q000032:analysis
D006025:Glycosaminoglycans; D006595:Hexosamines; D009088:Mucoproteins; D005643:Fucose; D014994:Xylose; D005947:Glucose; D008358:Mannose; D005690:Galactose