title: Protein-polyaccharide of chicken cartilage and chondrocyte cell cultures.
abstract: The nature of the matrix produced by embryonic chicken chondrocytes in cell culture was studied, and compared with adult and embryonic chicken cartilage. Adult chicken cartilage contains a protein-polysaccharide easily extracted with EDTA-sodium chloride at 4 degrees C. Purification of this macromolecule on Bio-Gel P-300 and Bio-Gel A-50m yielded a progressively more homogeneous species in the ultracentrifuge. It contained mostly chondroitin 4-sulphate, some chondroitin 6-sulphate, and keratan sulphate. Embryonic chicken cartilage was previously shown to contain mostly chondroitin 4-sulphate, some chondroitin 6-sulphate and essentially no keratan sulphate. The matrix produced in chondrocyte cell cultures was shown to contain a protein-polysaccharide with alkali-labile linkages of chondroitin 4-sulphate to the protein core. A fraction was isolated from the matrix with many properties of keratan sulphate.
mesh_terms: D000468:Alkalies; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002356:Cartilage / Q000032:analysis* / Q000166:cytology; D002642:Chick Embryo; D002645:Chickens; D002807:Chondroitin / Q000032:analysis; D002845:Chromatography; D002850:Chromatography, Gel; D046508:Culture Techniques; D004492:Edetic Acid; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D012965:Sodium Chloride; D014461:Ultracentrifugation
chemical_list: D000468:Alkalies; D000596:Amino Acids; D006025:Glycosaminoglycans; D012965:Sodium Chloride; D002807:Chondroitin; D004492:Edetic Acid