The glycosaminoglycans of neonatal rat skin.
The acid glycosaminoglycans were extracted from the skins of young rats less than 1 day post partum. The isolated products were fractionated by a cetylpyridinium chloride-cellulose column technique and identified by chemical analysis, electrophoretic mobility and susceptibility to testicular hyaluronidase digestion. Hyaluronic acid (56%) dermatan sulphate (15.6%) and chondroitin 6-sulphate (9.1%) were the major components, but chondroitin 4-sulphate, heparan sulphate and heparin were also present, together with two further fractions tentatively suggested to be a heparan sulphate-like fraction and a dermatan sulphate fraction, both of short chain length or low degree of sulphation.
D000818:Animals; D000831:Animals, Newborn; D002807:Chondroitin / Q000032:analysis; D002855:Chromatography, Thin Layer; D004586:Electrophoresis; D006025:Glycosaminoglycans / Q000032:analysis*; D006493:Heparin / Q000032:analysis; D006595:Hexosamines / Q000032:analysis; D006820:Hyaluronic Acid / Q000032:analysis; D006821:Hyaluronoglucosaminidase; D051381:Rats; D012867:Skin / Q000032:analysis*; D013431:Sulfates / Q000032:analysis
D006025:Glycosaminoglycans; D006595:Hexosamines; D013431:Sulfates; D006820:Hyaluronic Acid; D006493:Heparin; D002807:Chondroitin; D006821:Hyaluronoglucosaminidase