Radial immunodiffusion of chicken serum proteins. I. Standardization of optimal test procedures.
Optimal conditions were determined for the quantitaion of chicken serum albumin, conalbumin, IgG and IgM by the radial immunodiffusion test. The best diluent was 0.15 M phosphate buffered saline, pH 7.2. The optimal concentration of the rabbit antiserum in the agar plate was inversely related to the molecular weight of the protein under study. The incubation time required for maximum ring formation was directly related to the molecular weight of the proteins under study. The reproducibility of the tests was evaluated using stored and fresh antiserum-agar plates.
D000818:Animals; D000941:Antigens / Q000032:analysis / Q000592:standards; D001798:Blood Proteins / Q000032:analysis* / Q000592:standards; D002021:Buffers; D002645:Chickens; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera / Q000032:analysis / Q000592:standards; D005779:Immunodiffusion / Q000592:standards*; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000032:analysis / Q000592:standards; D007075:Immunoglobulin M / Q000032:analysis; D007201:Indicator Dilution Techniques; D008970:Molecular Weight; D010710:Phosphates; D011817:Rabbits; D012709:Serum Albumin / Q000032:analysis / Q000592:standards
D000941:Antigens; D001798:Blood Proteins; D002021:Buffers; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D010710:Phosphates; D012709:Serum Albumin