The addition of water to the Zimmermann reagent to reduce the background of paper chromatograms developed mechanically.
D015068:17-Ketosteroids / Q000032:analysis*; D000880:Anthraquinones; D002854:Chromatography, Paper; D003687:Dehydroepiandrosterone / Q000032:analysis; D006813:Humidity; D007202:Indicators and Reagents; D013451:Sulfonic Acids; D014867:Water
D015068:17-Ketosteroids; D000880:Anthraquinones; D007202:Indicators and Reagents; D013451:Sulfonic Acids; D014867:Water; D003687:Dehydroepiandrosterone