T antigens of bovine and canine adenoviruses demonstrated by immunofluorescence.
The intracellular development in acutely infected cells of bovine and canine adenovirus T antigens was followed by immunofluorescent staining. With both species of adenovirus, antigen was first detected as intranuclear pin-points at 18 hr postinfection and coalescence into large lobular masses was noticed by 24 hr. Cross-reactions between bovine 1 (nononcogenic) and bovine 3 (oncogenic) T antigens were not observed by the direct technique although the more sensitive indirect procedure did reveal cross-reactivity. Extensive cross-reactions were observed between the T antigens of the oncogenic canine hepatitis virus and the "nononcogenic" Toronto strain of canine adenovirus. The magnitude of these reactions places the two canine strains in the same T antigen subgroup. The canine and bovine T antigens were not stained by tumor antisera against any of the known human or simian T antigen subgroups. Antigen synthesis was not prevented by inhibitors of deoxyribonucleic acid synthesis although the appearance was altered markedly.
D000256:Adenoviridae / Q000276:immunology*; D000818:Animals; D000941:Antigens; D002417:Cattle; D002471:Cell Transformation, Neoplastic; D003168:Complement Fixation Tests; D046508:Culture Techniques; D003561:Cytarabine / Q000494:pharmacology; D004285:Dogs; D004622:Embryo, Mammalian; D005467:Floxuridine / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D007668:Kidney; D009374:Neoplasms, Experimental / Q000276:immunology*
D000941:Antigens; D005467:Floxuridine; D003561:Cytarabine