Transfection of lysostaphin-treated cells of Staphylococcus aureus.
After treatment with 1 unit of lysostaphin per ml for 3 min, two strains of Staphylococcus aureus, 233 and PS 44A HJD, were transfected with phenol-extracted deoxyribonucleic acid (DNA) from the staphylococcal bacteriophages, 53 and 44A HJD, respectively. The number of transfected cells was low in both systems, approximately two in 10(7) enzyme-treated cells. There was a saturation effect at high concentrations of DNA; optimal results were obtained at concentrations between 10 to 25 mug/ml. Growth curves and fluctuation tests indicated that cells of strain 44A HJD infected with phage, then converted to protoplasts by a 10-min treatment with lysostaphin, produce only one phage particle and lose their ability to lyse spontaneously in hypertonic media.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001433:Bacteriolysis; D002473:Cell Wall / Q000187:drug effects; D004279:DNA, Viral; D008248:Lysostaphin / Q000494:pharmacology; D011523:Protoplasts; D013210:Staphylococcus / Q000187:drug effects; D013204:Staphylococcus Phages; D014779:Virus Replication
D000900:Anti-Bacterial Agents; D004279:DNA, Viral; D008248:Lysostaphin