Mucopolysaccharide and protein--polysaccharide of a transplantable rat chondrosarcoma.
Two mucopolysaccharides, chondroitin 4-sulfate (97.8%) and hyaluronic acid (1.2%), were isolated after exhaustive proteolysis of a transplantable chondrosarcoma of the rat. The chondroitin 4-sulfate was fractionated into three fractions of varying degrees of sulfation and chain length. Keratan sulfate and chondroitin 6-sulfate were absent. Extraction of the fresh tumor gave two protein-polysaccharides of similar carbohydrate composition, one soluble in 0.5 M NaCl, the other insoluble. The latter was solubilized in 4 M guanidine.HCl. A dialyzable fraction from the 4 M guanidine solution may be responsible for the insolubility. Both protein-polysaccharides were antigenic and cross-reacted with similar fractions of bovine and human cartilage.
D000818:Animals; D002807:Chondroitin / Q000032:analysis*; D002813:Chondrosarcoma / Q000378:metabolism*; D002850:Chromatography, Gel; D003429:Cross Reactions; D006025:Glycosaminoglycans / Q000302:isolation & purification; D006146:Guanidines; D006595:Hexosamines / Q000032:analysis; D006820:Hyaluronic Acid / Q000032:analysis*; D006821:Hyaluronoglucosaminidase; D008297:Male; D009088:Mucoproteins / Q000032:analysis*; D009363:Neoplasm Proteins / Q000032:analysis*; D009368:Neoplasm Transplantation; D051381:Rats; D012513:Sarcoma, Experimental / Q000378:metabolism; D012995:Solubility; D013737:Testis / Q000201:enzymology; D014574:Uronic Acids / Q000032:analysis; D014994:Xylose / Q000032:analysis
D006025:Glycosaminoglycans; D006146:Guanidines; D006595:Hexosamines; D009088:Mucoproteins; D009363:Neoplasm Proteins; D014574:Uronic Acids; D006820:Hyaluronic Acid; D002807:Chondroitin; D014994:Xylose; D006821:Hyaluronoglucosaminidase