Evidence for the existence of a multichain proteoglycan of heparan sulphate.
1. Glycosaminoglycans were extracted with 2m-potassium chloride from bovine aorta and purified by precipitation with cetylpyridinium chloride from 0.5m-potassium chloride. The yield amounted to 24% of the total glycosaminoglycan content of the tissue. 2. After removal of chondroitin sulphate by digestion with testicular hyaluronidase, the residual glycosaminoglycan material (11% of the extracted polysaccharide) was fractionated by gel chromatography on Sephadex G-200. Two peaks (I and II) were obtained, the more retarded of which (II) corresponded to single polysaccharide chains. 3. The macromolecular properties of fraction I were investigated by repeated gel chromatography, after treatment of the fraction with alkali or digestion with papain. In both cases the elution position of fraction I was shifted towards that of the single polysaccharide chains. 4. Analysis of fraction I showed approximately equal amounts of heparan sulphate and dermatan sulphate. It is concluded that these glycosaminoglycans both occur in the aortic wall as multichain proteoglycans.
D000818:Animals; D001011:Aorta / Q000032:analysis; D002417:Cattle; D002845:Chromatography; D004586:Electrophoresis; D006023:Glycoproteins / Q000032:analysis*; D006025:Glycosaminoglycans / Q000032:analysis*; D006493:Heparin / Q000032:analysis*; D006821:Hyaluronoglucosaminidase; D010206:Papain
D006023:Glycoproteins; D006025:Glycosaminoglycans; D006493:Heparin; D006821:Hyaluronoglucosaminidase; D010206:Papain