title: Protein A mutants of Staphylococcus aureus.
abstract: Staphylococcus aureus Cowan I was exposed to nitrosoguanidine or ethyl-methanesulfonate, and survivors were screened on nutrient agar plates containing rabbit anti-protein A serum for loss of protein A production. More than half of all protein A-deficient mutants also lacked nuclease, coagulase, alpha hemolysin, fibrinolysin, mannitol utilization, and the phage-type pattern. Mutants with a spectrum of these properties were also isolated. Induced or spontaneous reversions of the mutants were observed. The properties of the protein A-deficient mutants suggest that synthesis or release (or both) of a number of extracellular products of S. aureus is controlled by a common regulatory mechanism.
mesh_terms: D000166:Acridines; D000473:Alkanes; D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis*; D001434:Bacteriophage Typing; D003030:Coagulase / Q000096:biosynthesis; D003470:Culture Media; D003851:Deoxyribonucleases / Q000096:biosynthesis; D005341:Fibrinolysin / Q000096:biosynthesis; D005827:Genetics, Microbial; D006386:Hemagglutination Tests; D006460:Hemolysin Proteins / Q000096:biosynthesis; D007106:Immune Sera; D008353:Mannitol / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D009153:Mutagens; D009154:Mutation; D009604:Nitrosoguanidines; D011233:Precipitin Tests; D011817:Rabbits; D013210:Staphylococcus / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D013204:Staphylococcus Phages
chemical_list: D000166:Acridines; D000473:Alkanes; D001426:Bacterial Proteins; D003030:Coagulase; D003470:Culture Media; D006460:Hemolysin Proteins; D007106:Immune Sera; D009153:Mutagens; D009604:Nitrosoguanidines; D008353:Mannitol; D003851:Deoxyribonucleases; D005341:Fibrinolysin