[Effect of cations and DNP on ATPase activity in different tissue fractions of birds' brains].
ATP-ase activity and the effect of cations (Na+, K+, Mg2+, Ca2+) and DNP on its activity have been studied in avian brain homogenates and mitochondrial and supernatant fractions (microsomes + hyaloplasma). Highest levels of Na+ + K+ -stimulated ATP-ase activity has been shown in avian brain crude mitochondrial fraction, next in supernatant and lowest in homogenates. Following three-fold freezing and thawing, enzyme activity in homogenates and mitochondrial fractions increases considerably and decreases in the supernatant. Highest Mg2+ -stimulated ATP-ase activity is found in avian brain homogenates, next in mitochondrial fractions and supernatants. Mg2+ -stimulated ATP-ase activity is not changed considerably following three-fold freezing and thawing of brain preparations. Highest Ca2+ -stimulated ATP-ase activity has been observed in the supernatant fraction and its activity is considerably reduced following freezing and thawing. Avian brain mitochondrial fractions have high DNP-stimulated ATP-ase activity. Following freezing and thawing of brain preparations DNP-stimulated ATP-ase activity is not changed considerably. The results obtained indicate that in avian brain homogenates and other preparations, cation and DNP-stimulated ATP-ase have different activities. Three-fold freezing and thawing of brain tissue preparations affect these activities differentially.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000648:ultrastructure; D002118:Calcium / Q000494:pharmacology; D002412:Cations / Q000494:pharmacology*; D002645:Chickens / Q000378:metabolism*; D004140:Dinitrophenols / Q000494:pharmacology*; D005615:Freezing; D008274:Magnesium / Q000494:pharmacology; D008928:Mitochondria / Q000201:enzymology; D011188:Potassium / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology
D002412:Cations; D004140:Dinitrophenols; D012964:Sodium; D000251:Adenosine Triphosphatases; D008274:Magnesium; D011188:Potassium; D002118:Calcium
Vliianie kationov i DNF na ATF-aznuiu aktivnost' v razlichnykh fraktsiiakh tkani mozga ptits.