The molecular-weight distribution of glycosaminoglycans.
1. A rapid and sensitive method for the accurate estimation of the molecular-weight distribution of keratan sulphate and chondroitin sulphate isolated from adult bovine nasal septum and intervertebral disc is described. The method utilizes gel chromatography of reductively labelled glycosaminoglycan and end-group estimation of number-average molecular weight for each fraction across the peak of eluted glycosaminoglycan. 2. Chain-length distribution data obtained by this procedure are used to evaluate mechanisms of chondroitin sulphate biosynthesis.
D000818:Animals; D002356:Cartilage / Q000032:analysis; D002417:Cattle; D002807:Chondroitin / Q000032:analysis / Q000096:biosynthesis; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D006025:Glycosaminoglycans / Q000032:analysis*; D007403:Intervertebral Disc / Q000032:analysis; D046911:Macromolecular Substances; D008722:Methods; D008970:Molecular Weight; D009300:Nasal Septum / Q000032:analysis; D013464:Sulfuric Acids / Q000032:analysis
D006025:Glycosaminoglycans; D046911:Macromolecular Substances; D013464:Sulfuric Acids; D002807:Chondroitin