Extraction of cartilage protein-polysaccharides with inorganic salt solutions.
1. Bovine nasal cartilage was extracted with inorganic salt solutions of various ionic strengths. The efficiency of extraction of protein-polysaccharide from the tissue was determined for each extraction. The results confirm and enlarge earlier observations (Sajdera & Hascall, 1969). 2. The chloride salts of lanthanide metals extract high yields of protein-polysaccharide from the tissue at much lower concentrations than was achieved with univalent and bivalent salts. 3. The lanthanum salt of extracted protein-polysaccharide precipitates when the concentration of LaCl(3) is decreased. Precipitation is complete in the presence of 0.05m-LaCl(3). This finding is relevant to the interpretation of earlier observations on the effect of LaCl(3) on elastic recovery of articular cartilage after compression (Sokoloff, 1963). 4. A linear relationship was found between the concentration at which a particular salt is maximally effective in solubilizing protein-polysaccharide from the tissue and the enthalpy of hydration of the cation of the salt. On the basis of this relationship a hypothesis is proposed to explain the characteristic protein-polysaccharide-extraction profiles exhibited by inorganic salt solutions.
D000818:Animals; D001464:Barium; D002122:Calcium Chloride; D002356:Cartilage / Q000032:analysis*; D002358:Cartilage, Articular / Q000187:drug effects; D002417:Cattle; D002563:Cerium; D002586:Cesium; D011232:Chemical Precipitation; D002712:Chlorides / Q000494:pharmacology; D006025:Glycosaminoglycans / Q000302:isolation & purification*; D007811:Lanthanum / Q000494:pharmacology; D008094:Lithium; D008274:Magnesium; D008956:Models, Chemical; D009666:Nose; D009994:Osmolar Concentration; D011189:Potassium Chloride; D011487:Protein Conformation; D012492:Salts; D012965:Sodium Chloride; D012995:Solubility; D013816:Thermodynamics
D002712:Chlorides; D006025:Glycosaminoglycans; D012492:Salts; D002586:Cesium; D001464:Barium; D002563:Cerium; D012965:Sodium Chloride; D011189:Potassium Chloride; D007811:Lanthanum; D008094:Lithium; D008274:Magnesium; D002122:Calcium Chloride