A direct fluorescent antibody test for identification of group E streptococci.
A rapid and specific direct fluorescent antibody test was developed for the identification of group E Streptococci (GES). Tests for specificity included an inhibition test and application of the conjugate to smears of Staphylococcus aureus, Pasteurella multocida, and streptococci of Lancefield's groups, A, B, C, D, E (types I, IV and untypeable), F and G. With the inhibition test, a specific blocking reaction occurred and the conjugate cross-reacted only with group C Streptococci (GCS). Adsorption with GCS eliminated the cross-reaction and rendered the conjugate specific for GES.
D000327:Adsorption; D000818:Animals; D000907:Antibodies, Bacterial; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D005620:Freund's Adjuvant / Q000008:administration & dosage; D007267:Injections; D007275:Injections, Intravenous; D010325:Pasteurella / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D013210:Staphylococcus / Q000276:immunology; D013291:Streptococcus / Q000276:immunology / Q000302:isolation & purification*
D000907:Antibodies, Bacterial; D005620:Freund's Adjuvant