Use of liquid nitrogen and high ionic strength for the isolation of functional polyribosomes from Neurospora crassa.
D002250:Carbon Radioisotopes; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D003080:Cold Temperature; D005656:Fungal Proteins / Q000096:biosynthesis; D008722:Methods; D009491:Neurospora / Q000166:cytology*; D009492:Neurospora crassa / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D009584:Nitrogen; D009994:Osmolar Concentration; D010649:Phenylalanine / Q000378:metabolism; D011132:Polyribosomes / Q000201:enzymology / Q000378:metabolism; D011189:Potassium Chloride; D012260:Ribonucleases / Q000032:analysis; D013997:Time Factors; D014316:Tritium
D002250:Carbon Radioisotopes; D005656:Fungal Proteins; D014316:Tritium; D010649:Phenylalanine; D011189:Potassium Chloride; D012260:Ribonucleases; D009584:Nitrogen