title: Informational DNA synthesis distinguished from that of nuclear DNA by inhibitors of DNA synthesis.
abstract: With inhibitors of DNA synthesis used in the presence of ethidium bromide, it has been possible to distinguish between synthesis of informational DNA (I-DNA) and that of nuclear DNA. Hydroxyurea depresses I-DNA synthesis preferentially but does not affect DNA transport between cell compartments. 5-Fluorodeoxyuridine and cytosine arabinoside reduce synthesis of I-DNA to a much lesser degree than that of nuclear DNA.
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D002642:Chick Embryo; D003561:Cytarabine / Q000494:pharmacology*; D003593:Cytoplasm / Q000378:metabolism; D004247:DNA / Q000032:analysis / Q000096:biosynthesis*; D003864:Depression, Chemical; D005467:Floxuridine / Q000494:pharmacology*; D005815:Genetic Code; D006918:Hydroxyurea / Q000494:pharmacology*; D009132:Muscles / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
chemical_list: D005467:Floxuridine; D003561:Cytarabine; D014316:Tritium; D004247:DNA; D013936:Thymidine; D006918:Hydroxyurea