Endotoxin induced disseminated intravascular coagulation in cattle.
Endotoxin administered intravenously to a group of four calves resulted in disseminated intravascular coagulation. A sublethal dose of piromen, a commercially available Pseudomonas spp endotoxin, was used. Serial measurements of total plasma fibrinogen, soluble fibrin levels, ethanol gelation tests, protamine sulfate tests, fibrinogen-fibrin-related antigen (FR-antigen) and prothrombin and thrombin times were done.Initial depression of plasma fibrinogen with a nadir of about 40% of pre-endotoxin levels at eight to 11 hours post-endotoxin (+8 to +11 hours) followed by an overcompensation to 180% at +60 to +108 hours was shown. Soluble fibrin was demonstrated in plasma from +2 to +22 hours with a peak of 100-114 mg/100 ml at +4 to +9 hours. Positive plasma ethanol gelation and protamine sulfate tests, as well as the presence of serum FR-antigen, occurred consistently following endotoxin administration. Significant increases in prothrombin times (PT) from +4 to +40 hours and in thrombin times (TT) from +4 to +16 hours were demonstrated. The peak increase of PT at +8 to +10 hours was 180%. The peak increase of TT at +6 to +9 hours was 260-290%.
D000818:Animals; D000941:Antigens; D001780:Blood Coagulation Tests; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000209:etiology*; D004211:Disseminated Intravascular Coagulation / Q000097:blood / Q000209:etiology / Q000662:veterinary*; D004731:Endotoxins / Q000008:administration & dosage / Q000633:toxicity*; D000431:Ethanol; D005337:Fibrin / Q000032:analysis / Q000276:immunology; D005340:Fibrinogen / Q000032:analysis / Q000276:immunology; D005779:Immunodiffusion; D007275:Injections, Intravenous; D008297:Male; D008722:Methods; D011479:Protamines; D011517:Prothrombin Time; D013431:Sulfates
D000941:Antigens; D004731:Endotoxins; D011479:Protamines; D013431:Sulfates; D000431:Ethanol; D005337:Fibrin; D005340:Fibrinogen