Coupling between mitochondrial mutation and energy transduction.
Upon incubation with ethidium bromide (Etd Br) isolated mitochondria of Saccharomyces cerevisiae have been shown to be capable of performing five novel reactions: (a) a single scission of their DNA (mtDNA) coincident with (b) the incorporation of Etd Br into the product (mtDNA --> 2 mtDNA'-Etd Br); (c) an energy- (and probably ATP-) requiring degradation of mtDNA'-Etd Br to acid-labile products. These reactions acting in series generate (d) a DNase dependent on both Etd Br and an energy supply with mtDNA'-Etd Br as an obligatory intermediate. Coincident with (d) there occurs (e) an activation of adenosinetriphosphatase. Experiments with specific inhibitors suggest that the enzyme responsible is the mitochondrial adenosinetriphosphatase complex itself, and that it and the ability to carry out reactions (a) through (e) are tightly coupled to the energy-transducing functions of the particle.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D001965:Bromides / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000378:metabolism*; D004734:Energy Metabolism; D004996:Ethidium / Q000378:metabolism* / Q000494:pharmacology; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism*; D009154:Mutation / Q000187:drug effects*; D012441:Saccharomyces cerevisiae / Q000166:cytology
D001965:Bromides; D004272:DNA, Mitochondrial; D000251:Adenosine Triphosphatases; D004996:Ethidium