A fluorescent modification of adenosine triphosphate with activity in enzyme systems: 1,N 6 -ethenoadenosine triphosphate.
A new, highly fluorescent adenosine triphophate (ATP) analog, 1,N(6) ethenoadenosine triphosphate, has been synthesized. Its fluorescence properties, including the long fluorescence lifetime and the possibility of detection at very low concentrations, in conjunction with its activity in the representative enzyme systems here reported, make it a valuable probe of enzymic mechanism and structure.
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000032:analysis / Q000138:chemical synthesis* / Q000378:metabolism; D005453:Fluorescence; D006593:Hexokinase / Q000378:metabolism; D007093:Imidazoles / Q000032:analysis / Q000138:chemical synthesis / Q000378:metabolism; D009132:Muscles / Q000201:enzymology; D010732:Phosphofructokinase-1 / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism; D013057:Spectrum Analysis; D015003:Yeasts / Q000201:enzymology
D007093:Imidazoles; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D006593:Hexokinase; D010732:Phosphofructokinase-1; D011770:Pyruvate Kinase