Proliferation of B and T cells in mixed lymphocyte cultures.
Electrophoretically fractionated CBA/Ca spleen T cells alone respond to allogeneic cells in one-way MLC and to PHA. They do not respond to E. coli LPS. B cells alone do not respond to allogeneic cells nor to PHA, but do respond to LPS. When karyotypically distinguishable syngeneic mixtures of T and B lymphocytes are stimulated with allogeneic cells, at the most 5% of mitoses on 5-9th culture day are of B cell origin. This indicates that B cells are not substantially recruited to proliferate in the MLC.
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002478:Cells, Cultured; D004586:Electrophoresis; D007621:Karyotyping; D037102:Lectins / Q000494:pharmacology; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008815:Mice, Inbred Strains; D008938:Mitosis; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology
D037102:Lectins