Purification and amino acid analysis of an active site peptide from thymidylate synthetase containing covalently bound 5-fluoro-2'-deoxyuridylate and methylenetetrahydrofolate.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001665:Binding Sites; D002250:Carbon Radioisotopes; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004352:Drug Resistance, Microbial; D004590:Electrophoresis, Paper; D005467:Floxuridine; D007780:Lacticaseibacillus casei / Q000187:drug effects / Q000201:enzymology; D008727:Methotrexate / Q000494:pharmacology; D008780:Methyltransferases; D010455:Peptides; D011402:Pronase; D011485:Protein Binding; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013763:Tetrahydrofolates; D013940:Thymidylate Synthase / Q000302:isolation & purification; D014316:Tritium; D014500:Uracil Nucleotides
D000596:Amino Acids; D002250:Carbon Radioisotopes; D010455:Peptides; D013763:Tetrahydrofolates; D014500:Uracil Nucleotides; D005467:Floxuridine; D014316:Tritium; D008780:Methyltransferases; D013940:Thymidylate Synthase; D011402:Pronase; D008727:Methotrexate