An improved method for the production of Neurospora crassa spheroplasts and a new method for measuring transport in fragile cells.
D001693:Biological Transport, Active; D002250:Carbon Radioisotopes; D002458:Cell Fractionation / Q000379:methods; D002473:Cell Wall / Q000032:analysis; D002499:Centrifugation, Density Gradient; D002686:Chitin / Q000032:analysis; D005069:Evaluation Studies as Topic; D005947:Glucose / Q000378:metabolism; D005966:Glucuronidase; D008722:Methods; D009491:Neurospora / Q000166:cytology* / Q000648:ultrastructure; D009492:Neurospora crassa / Q000032:analysis / Q000166:cytology* / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate; D013013:Sorbose / Q000378:metabolism; D013104:Spheroplasts / Q000378:metabolism / Q000648:ultrastructure; D013997:Time Factors
D002250:Carbon Radioisotopes; D002686:Chitin; D012967:Sodium Dodecyl Sulfate; D005966:Glucuronidase; D005947:Glucose; D013013:Sorbose