title: Adenosine triphosphatase associated with adenosine triphosphate-dependent deoxyribonuclease (recB-recC enzyme-E. coli-ATP to phosphodiester hydrolysis ratio-DNA-dependent ATPase activity).
abstract: An ATPase activity that is completely dependent on DNA is associated with the ATP-dependent DNase (recB-recC enzyme) purified from Escherichia coli. There is a strong correlation between the ATPase and the DNase activities under various assay conditions. With E. coli DNA as substrate, 8-9 molecules of ATP are hydrolyzed to ADP and inorganic phosphate for every phosphodiester bond hydrolyzed by the DNase. The possible functional relationship of the ATPase and DNase activities is discussed.
mesh_terms: D000178:Acrylamides; D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002848:Chromatography, DEAE-Cellulose; D003090:Coliphages; D004279:DNA, Viral / Q000302:isolation & purification; D003851:Deoxyribonucleases / Q000378:metabolism*; D004586:Electrophoresis; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism; D005809:Genes, Regulator; D006358:Hot Temperature; D010759:Phosphorus Isotopes; D013307:Streptomycin; D014461:Ultracentrifugation
chemical_list: D000178:Acrylamides; D004279:DNA, Viral; D010759:Phosphorus Isotopes; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D003851:Deoxyribonucleases; D000251:Adenosine Triphosphatases; D013307:Streptomycin