Glycosaminoglycan synthesis in mouse mastocytoma.
The glycosaminoglycan synthesis in Furth solid mastocytoma tissue has been studied. Approx. 10% of the polysaccharide isolated after incubation in vitro with [(14)C]-glucosamine was digestible with chondroitinase ABC and the product of digestion was identified as 2-acetamido-2-deoxy-3-O-(beta-d-gluco-4-enepyranosyluronic acid)-4-O-sulpho-d-galactose. Similarly, labelling of polysaccharide in vivo with (35)SO(4) (2-) followed by isolation of mast-cell fractions by density-gradient centrifugation on colloidal silica revealed the presence of a polysaccharide which migrated as did chondroitin sulphate on electrophoresis in barium acetate. Chondroitinase ABC produced the same digestion product as before. Finally, the presence of the UDP-N-acetylgalactosamine-chondroitin 6-sulphate hexasaccharide N-acetylgalactosaminyltransferase previously implicated in chondroitin sulphate biosynthesis was demonstrated in microsomal particles from fractions of purified mast cells.
D000818:Animals; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D002807:Chondroitin; D002845:Chromatography; D004586:Electrophoresis; D005944:Glucosamine / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006595:Hexosamines / Q000378:metabolism; D008190:Lyases / Q000378:metabolism; D008407:Mast Cells; D012515:Mast-Cell Sarcoma / Q000378:metabolism*; D051379:Mice; D008861:Microsomes; D009374:Neoplasms, Experimental / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism; D013460:Sulfur Isotopes
D002247:Carbon Isotopes; D006025:Glycosaminoglycans; D006595:Hexosamines; D013431:Sulfates; D013460:Sulfur Isotopes; D002807:Chondroitin; D008190:Lyases; D005944:Glucosamine