Hemadsorption-negative plaque test: new assay for rubella virus revealing a unique interference.
A simple and rapid plaque procedure has been developed for detecting and accurately assaying rubella virus in a noncytopathic virus-cell relationship. Plaque-formation is based on the development, in individual cells infected with rubella virus, of a unique type of intrinsic interference to infection with Newcastle disease virus. Rubella virus-infected cells challenged with Newcastle disease virus and tested for hemadsorption 15 hours later stand out as hemadsorption-negative areas. Individual living cells infected with rubella virus can be resolved under conditions allowing standard cloning procedures. In principle, the hemadsorption-negative plaque test can be used to search for a new class of noncytopathic, non-hemadsorbing viruses-those that induce an intrinsic interference to infection by any hemadsorbing virus.
D000818:Animals; D029822:Enterovirus B, Human / Q000276:immunology; D000882:Haplorhini; D006383:Hemadsorption Inhibition Tests; D066298:In Vitro Techniques; D007668:Kidney; D009522:Newcastle disease virus / Q000276:immunology; D009975:Orthomyxoviridae / Q000276:immunology; D017955:Poliovirus / Q000276:immunology; D012412:Rubella virus / Q000276:immunology*; D014616:Vaccinia virus / Q000276:immunology; D014762:Viral Interference; D014776:Virus Cultivation