The subunits of chemically treated proteoglycan isolated from bovine nasal cartilage.
1. The light fraction of the proteoglycan of bovine nasal cartilage was split by treatment with 0.1m-hydrochloric acid in acetone. The products were separated by gel filtration on 4% agarose and two retarded fractions were detected and isolated. These two fractions were found to have a Stokes radius of 134 and 47 A respectively, as determined by calibration of the column against proteins of known hydrodynamic volumes. 2. The 47 A fraction had a protein content of 4% and a glucosamine/galactosamine ratio 1:23. The 134 A fraction had a protein content of 20% and a glucosamine/galactosamine ratio 1:4.8. 3. The results of the viscometric studies on both fractions suggested that the 134 A fraction alone exhibited the property of undergoing reversible pH-dependent aggregation with a transition point at pH4.9. 4. It was concluded that these fractions could represent subunits of the native cartilage proteoglycan.
D000096:Acetone; D000818:Animals; D002356:Cartilage / Q000032:analysis*; D002417:Cattle; D002850:Chromatography, Gel; D005688:Galactosamine / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis*; D006025:Glycosaminoglycans / Q000032:analysis*; D006851:Hydrochloric Acid; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D009300:Nasal Septum; D009997:Osmotic Pressure; D014783:Viscosity
D006023:Glycoproteins; D006025:Glycosaminoglycans; D000096:Acetone; D005688:Galactosamine; D005944:Glucosamine; D006851:Hydrochloric Acid