Evaluation of various media and the use of soil infusion in the production of Brucella abortus antigen.
Brucella abortus (ADRI 413) was grown on various synthetic, commercial, laboratory-formulated and soil infusion-supplemented agar media. Of 22 media tested, three soil infusion-supplemented media and a serum dextrose agar yielded more brucella antigen than the meat infusion agar, the standard reference medium. Results from agglutination and stability tests indicated that antigens produced from most of the laboratory-formulated media and the soil infusion-supplemented agars compared favourably with that derived from the meat infusion agar. The presence of meat infusion or commercial beef extract appeared to be a basic requirement for producing satisfactory and stable antigen. The growth enhancing effect of certain soil infusions seemed to be associated with the soil organic matter fraction. A significant correlation (p>.05) was obtained between the yield of brucella antigen and the glutamic acid content of the soil infusions. With proper screening, media containing, among other components, beef extract and soil infusions may be of value particularly in areas where the limited supply of fresh meat would make meat infusion agar uneconomical in the production of B. abortus (ADRI 413) antigen.
D000362:Agar; D000372:Agglutination Tests; D000942:Antigens, Bacterial / Q000302:isolation & purification; D001431:Bacteriological Techniques; D002003:Brucella abortus / Q000254:growth & development / Q000276:immunology*; D003470:Culture Media; D008460:Meat; D012987:Soil; D013395:Sucrose; D014675:Vegetables
D000942:Antigens, Bacterial; D003470:Culture Media; D012987:Soil; D013395:Sucrose; D000362:Agar