Cytochalasin B, its interaction with actin and actomyosin from muscle (cell movement-microfilaments-rabbit striated muscle).
Cytochalasin B, an alkaloid that inhibits a wide variety of cellular movements, interacts with actomyosin, the contractile protein complex of striated muscle. This interaction causes a decrease in viscosity of the actomyosin complex and an inhibition of acto-heavy meromyosin ATPase activity of at least 60%. Cytochalasin B does not affect the viscosity of myosin nor the ATPase activity of heavy meromyosin, suggesting that the drug might interact directly with the actin moiety of the actomyosin complex. Indeed, as judged by viscometry, there is a strong interaction of cytochalasin B with actin, at nearly stoichiometric concentrations. Myosin appears to compete with cytochalasin for binding to actin.
D000199:Actins; D000205:Actomyosin; D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors; D000818:Animals; D002465:Cell Movement / Q000187:drug effects*; D003956:Dialysis; D004789:Enzyme Activation / Q000187:drug effects; D046911:Macromolecular Substances; D009124:Muscle Proteins; D009183:Mycotoxins / Q000494:pharmacology*; D009218:Myosins; D009994:Osmolar Concentration; D011485:Protein Binding / Q000187:drug effects; D011817:Rabbits; D014783:Viscosity
D000199:Actins; D046911:Macromolecular Substances; D009124:Muscle Proteins; D009183:Mycotoxins; D000205:Actomyosin; D000251:Adenosine Triphosphatases; D009218:Myosins