Dog red blood cells. Adjustment of density in vivo.
Red blood cells from mature dogs contain less Na and more K than would be the case if they were in Donnan equilibrium with plasma. They have no ouabain-sensitive Na pump, and their membranes are deficient in Na, K-ATPase. Experiments are reported in which dog red cells were first loaded with supranormal quantities of Na and water and then reinjected into the dog. Over the course of 26-40 h the Na- and water-loaded cells returned to a normal state of hydration as judged by their density. It is concluded that dog red cells possess some means of correcting their swollen status in vivo, despite their lack of a ouabain-sensitive cation transport apparatus.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D002712:Chlorides / Q000097:blood*; D004285:Dogs; D004912:Erythrocytes / Q000201:enzymology / Q000502:physiology*; D006801:Humans; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000097:blood*; D012964:Sodium / Q000097:blood*; D012975:Sodium Isotopes; D013997:Time Factors; D014882:Water-Electrolyte Balance
D002712:Chlorides; D012975:Sodium Isotopes; D010042:Ouabain; D012964:Sodium; D000251:Adenosine Triphosphatases; D011188:Potassium