Stimulation of RNA transcription from pea and corn DNA by protein retained on sepharose coupled to 2,4-dichlorophenoxyacetic acid.
When crude extracts from pea or corn shoots are passed through a column of agarose coupled to a derivative of the synthetic plant hormone 2,4-dichlorophenoxyacetic acid, certain protein fractions are retained, whereas unmodified agarose adsorbs no protein. Appropriate elution yields a protein factor that stimulates DNA-dependent RNA synthesis (supported by Escherichia coli polymerase) by 40-200%. Preliminary evidence suggests that the factor may be influencing initiation of RNA chains.
D015084:2,4-Dichlorophenoxyacetic Acid; D000085:Acetates; D000251:Adenosine Triphosphatases / Q000032:analysis; D000255:Adenosine Triphosphate / Q000378:metabolism; D002247:Carbon Isotopes; D002845:Chromatography; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism*; D004586:Electrophoresis; D004926:Escherichia coli / Q000201:enzymology; D005815:Genetic Code; D010636:Phenols; D010759:Phosphorus Isotopes; D010940:Plant Proteins / Q000032:analysis* / Q000494:pharmacology; D010945:Plants, Edible / Q000032:analysis / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D011485:Protein Binding; D012313:RNA / Q000096:biosynthesis*; D012316:RNA Nucleotidyltransferases / Q000494:pharmacology; D012260:Ribonucleases / Q000032:analysis; D012293:Rifampin / Q000494:pharmacology; D003313:Zea mays / Q000032:analysis / Q000378:metabolism*
D000085:Acetates; D002247:Carbon Isotopes; D010636:Phenols; D010759:Phosphorus Isotopes; D010940:Plant Proteins; D015084:2,4-Dichlorophenoxyacetic Acid; D012313:RNA; D000255:Adenosine Triphosphate; D004247:DNA; D012316:RNA Nucleotidyltransferases; D012260:Ribonucleases; D000251:Adenosine Triphosphatases; D011188:Potassium; D012293:Rifampin