The subunits and biological activity of polymorphic forms of tropomyosin.
1. Free thiol groups were shown to be essential for tropomyosin to effect maximum inhibition of the Ca(2+)-stimulated ATPase (adenosine triphosphatase) of desensitized actomyosin but not for its activity in the regulatory-protein system. 2. The activity of tropomyosin on the Mg(2+)-stimulated ATPase in the regulatory-protein system was more susceptible to enzymic digestion and thermal denaturation than its effect on the Ca(2+)-stimulated ATPase of actomyosin. 3. Rabbit skeletal tropomyosin migrated as two distinct electrophoretic components in the presence of sodium dodecyl sulphate and urea and as four components on isoelectric focusing in urea. 4. The two main subunits present in rabbit skeletal tropomyosin, which have been named the alpha- and beta-chains, were separated by chromatography on CM-cellulose in urea at pH4.0. They were shown to be virtually identical in amino acid composition, except for their cysteine contents. The alpha(2) and beta(2) forms of tropomyosin possessed all the biological activities characteristic of normal tropomyosin preparations. 5. In skeletal muscle the alpha and beta components of tropomyosin were present in the proportion of 4:1. Somewhat lower ratios were obtained in skeletal muscle of sheep, pig and cow. 6. Tropomyosin isolated from cardiac muscle and Pecten maximus adductor muscle migrated as one band only. These tropomyosins possessed similar biological activities to those isolated from skeletal muscle.
D000205:Actomyosin; D000251:Adenosine Triphosphatases; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002118:Calcium; D002250:Carbon Radioisotopes; D002417:Cattle; D002845:Chromatography; D003545:Cysteine / Q000032:analysis; D004586:Electrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D008274:Magnesium; D008974:Mollusca; D009132:Muscles / Q000032:analysis; D009206:Myocardium / Q000032:analysis; D010206:Papain; D011817:Rabbits; D012967:Sodium Dodecyl Sulfate; D013696:Temperature; D014335:Tropomyosin / Q000302:isolation & purification; D014508:Urea
D000596:Amino Acids; D002250:Carbon Radioisotopes; D014335:Tropomyosin; D012967:Sodium Dodecyl Sulfate; D014508:Urea; D000205:Actomyosin; D010206:Papain; D000251:Adenosine Triphosphatases; D008274:Magnesium; D003545:Cysteine; D002118:Calcium