Activation of membrane-bound adenylate cyclase by glucagon in Neurospora crassa.
Membrane-bound adenylate cyclase in Neurospora crassa is activated by glucagon. Half-maximal effect is observed at hormone concentrations of about 10 nM. After solubilization of the enzyme with Lubrol-PX, the glucagon effect is lost. Incubation of neurospora cells with glucagon leads to a decrease in the activity of glycogen synthetase (EC 2.4.1.11) and to an increase in the activity of glycogen phosphorylase (EC 2.4.1.1) and in the rate of glycogenolysis.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000262:Adenylyl Cyclases / Q000378:metabolism*; D002247:Carbon Isotopes; D002462:Cell Membrane / Q000201:enzymology*; D003902:Detergents; D004347:Drug Interactions; D004789:Enzyme Activation / Q000187:drug effects; D005934:Glucagon / Q000494:pharmacology*; D006006:Glycogen Synthase / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000494:pharmacology; D007700:Kinetics; D008345:Manganese / Q000494:pharmacology; D009491:Neurospora / Q000201:enzymology*; D009492:Neurospora crassa / Q000201:enzymology; D009994:Osmolar Concentration; D010759:Phosphorus Isotopes; D006005:Phosphorylases / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D013268:Stimulation, Chemical
D002247:Carbon Isotopes; D003902:Detergents; D010759:Phosphorus Isotopes; D008345:Manganese; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D005934:Glucagon; D006005:Phosphorylases; D006006:Glycogen Synthase; D000262:Adenylyl Cyclases