Mouse spleen cells cultivated in homologous serum can make primary antibodies in vitro provided the T-cells are non-specifically activated.
D000818:Animals; D000917:Antibody Formation; D000942:Antigens, Bacterial; D001402:B-Lymphocytes; D001769:Blood; D002417:Cattle; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis; D004912:Erythrocytes; D004926:Escherichia coli; D005333:Fetus; D007518:Isoantibodies; D008070:Lipopolysaccharides; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice / Q000276:immunology; D012756:Sheep / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D013601:T-Lymphocytes; D013934:Thymectomy; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D000942:Antigens, Bacterial; D007518:Isoantibodies; D008070:Lipopolysaccharides; D014316:Tritium; D004247:DNA; D013936:Thymidine