A comparative study of the rabbit and pig gut loop systems for the assay of Escherichia coli enterotoxin.
A comparison was made between segments of pig and rabbit small intestine in their response to heat-labile (LT) and heat-stable (ST) preparations from porcine enteropathogenic Escherichia coli. Either whole cell lysates or dialysed broth culture supernatants were used as sources of LT and soft agar culture fluids as a source of ST. Whole cell lysates of all thirteen LT-producing E. coli strains tested regularly elicited fluid accumulation in rabbit gut loops. Whole cell lysates of certain E. coli strains considered to be nonenteropathogenic in pigs could also elicit a positive response in rabbit gut loops. When graded doses of LT were tested in pig and rabbit gut loops, the rabbit was more sensitive and is therefore considered preferable to the pig for quantitation of LT. In the rabbit, upper (jejunal) and lower (ileal) small intestine were compared for their response to LT and it was found that ileal loops were twice as sensitive but more prone to false positive reactions. When soft agar culture fluids of several enteropathogenic E. coli strains were tested in the rabbit, the response was inconsistent, and it was concluded that the rabbit is unsuitable for the assay of the heat-stable enterotoxin.
D000818:Animals; D001681:Biological Assay; D002474:Cell-Free System; D003470:Culture Media; D004768:Enterotoxins / Q000032:analysis*; D004926:Escherichia coli / Q000032:analysis / Q000254:growth & development / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000175:diagnosis / Q000662:veterinary*; D006358:Hot Temperature; D007082:Ileum / Q000187:drug effects; D007421:Intestine, Small / Q000187:drug effects*; D007583:Jejunum / Q000187:drug effects; D008722:Methods; D011817:Rabbits; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*
D003470:Culture Media; D004768:Enterotoxins