Improved rapid plate method for the isolation of bacteriophages from lysogenic bacteria.
A modification of a recently reported rapid plate method for the isolation of bacteriophages from lysogenic bacteria is described. The velveteen replica plate technique was used for inoculation of mitomycin C-induced colonies onto agar plates, and tetrazolium chloride was used to enhance detection of phage activity on replicated indicator plates.
D000362:Agar; D008242:Lysogeny; D008722:Methods; D008937:Mitomycins; D013210:Staphylococcus; D013204:Staphylococcus Phages / Q000254:growth & development / Q000302:isolation & purification*; D013778:Tetrazolium Salts; D014779:Virus Replication
D008937:Mitomycins; D013778:Tetrazolium Salts; D000362:Agar