title: Polysomes, ribonucleic acid, and protein synthesis during germination of Neurospora crassa conidia.
abstract: The level of polysomes in ungerminated conidia of Neurospora crassa depends on the method used to collect spores. Spores harvested and exposed to hydration contain 30% of their ribosomes as polysomes, whereas those not exposed to hydration contain only 3% of their ribosomes as polysomes. During the germination process, the percentage of the ribosomes which sediment as polysomes increases rapidly to a level of approximately 75% during the first 15 to 30 min of germination. This rapid increase has been shown to require a carbon source. During the first 30 min of germination, spores synthesize ribosomal ribonucleic acid (RNA) and heterogeneously sedimenting RNA, i.e., presumptive messenger RNA.
mesh_terms: D000225:Adenine / Q000378:metabolism; D002250:Carbon Radioisotopes; D002499:Centrifugation, Density Gradient; D003470:Culture Media; D005656:Fungal Proteins / Q000096:biosynthesis; D007930:Leucine / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D008851:Micropore Filters; D009491:Neurospora / Q000254:growth & development*; D009492:Neurospora crassa / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism*; D011691:Puromycin / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D012335:RNA, Ribosomal / Q000096:biosynthesis; D012260:Ribonucleases / Q000494:pharmacology; D013053:Spectrophotometry; D013172:Spores, Fungal / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D014316:Tritium; D014498:Uracil / Q000378:metabolism; D014867:Water
chemical_list: D002250:Carbon Radioisotopes; D003470:Culture Media; D005656:Fungal Proteins; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D014867:Water; D014316:Tritium; D011691:Puromycin; D014498:Uracil; D012313:RNA; D012260:Ribonucleases; D007930:Leucine; D008274:Magnesium; D000225:Adenine