Metabolism of C 19 -steroids by homogenates of normal rat and mouse adrenal tissue and of the Snell transplantable rat adrenocortical tumour 494.
C(19)-steroid metabolism in homogenates of adrenal tissue from rats and mice has been studied. Production of these compounds from [7alpha-(3)H]cholesterol by rat adrenal tissue appeared to follow a route independent of pregnenolone. The major products of [7alpha-(3)H]-dehydroepiandrosterone metabolism by rat adrenal tissue were 5alpha-reduced steroids, principally androsterone, epiandrosterone and 5alpha-androstanedione. No differences in metabolism of [7alpha-(3)H]dehydroepiandrosterone or [4-(14)C]pregnenolone were detected between adrenal tissue from Sprague-Dawley, Wistar and Osborne-Mendel rats, but experiments with the Snell rat adrenocortical tumour 494 showed that this tissue had low 5alpha-reductase activity. In contrast, the major products of [7alpha-(3)H]dehydroepiandrosterone metabolism by mouse adrenal tissue were 5beta-reduced steroids. Differences were observed between LACA and NH strains of mice in that there was a lower metabolism of androstenedione by NH mouse adrenal and a considerable difference in the proportions of aetiocholanolone and epiaetiocholanolone produced.
D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000311:Adrenal Glands / Q000378:metabolism*; D000731:Androstanes / Q000096:biosynthesis; D000738:Androsterone / Q000096:biosynthesis; D000818:Animals; D002247:Carbon Isotopes; D002784:Cholesterol / Q000378:metabolism; D003687:Dehydroepiandrosterone / Q000378:metabolism; D005043:Etiocholanolone / Q000096:biosynthesis; D066298:In Vitro Techniques; D007659:Ketones / Q000096:biosynthesis; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism; D011284:Pregnenolone / Q000378:metabolism; D051381:Rats; D013256:Steroids / Q000378:metabolism*; D014316:Tritium
D000731:Androstanes; D002247:Carbon Isotopes; D007659:Ketones; D013256:Steroids; D014316:Tritium; D003687:Dehydroepiandrosterone; D011284:Pregnenolone; D002784:Cholesterol; D005043:Etiocholanolone; D000738:Androsterone