title: Coupling of glycosaminoglycans to agarose beads (sepharose 4B).
abstract: 1. Heparin, heparan sulphate, chondroitin sulphate and dermatan sulphate were covalently attached to beads of agarose activated by cyanogen bromide. The bond is probably mediated by the amino group of a serine or peptide residue at the reducing end of the polysaccharide chain. 2. The uptake of glycosaminoglycan during the coupling procedure is about 0.9mg/ml of wet gel. However, direct analysis of washed and freeze-dried gels reveals that only about one-third of this amount is firmly attached to the gel. 3. The use of the gels for polysaccharidase analyses is exemplified by a hyaluronidase assay. Further applications, e.g. interaction studies and preparative purposes, are discussed.
mesh_terms: D000588:Amines; D000818:Animals; D002417:Cattle; D002807:Chondroitin; D003488:Cyanogen Bromide; D005612:Freeze Drying; D005782:Gels; D006025:Glycosaminoglycans; D006493:Heparin; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000032:analysis; D008722:Methods; D010455:Peptides; D011134:Polysaccharides; D012694:Serine; D013431:Sulfates; D013552:Swine
chemical_list: D000588:Amines; D005782:Gels; D006025:Glycosaminoglycans; D010455:Peptides; D011134:Polysaccharides; D013431:Sulfates; D012694:Serine; D006493:Heparin; D002807:Chondroitin; D006821:Hyaluronoglucosaminidase; D003488:Cyanogen Bromide