title: Nature of the interaction of chondroitin 4-sulphate and chondroitin sulphate-proteoglycan with collagen.
abstract: The electrostatic interaction of chondroitin sulphate and the chondroitin sulphate-proteoglycan with collagen was studied by chromatography of the glycosaminoglycan and the proteoglycan on a collagen gel. The observed binding between the macromolecules increased with decreasing pH and ionic strength, and was significant under physiological conditions. A study of the interaction between chondroitin sulphate and a preparation of soluble collagen, with a partition-equilibrium technique, afforded similar results.
mesh_terms: D000818:Animals; D002417:Cattle; D002807:Chondroitin / Q000378:metabolism*; D002845:Chromatography; D003094:Collagen / Q000378:metabolism*; D003911:Dextrans; D005782:Gels; D006023:Glycoproteins / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D046911:Macromolecular Substances; D011485:Protein Binding; D012995:Solubility; D013431:Sulfates / Q000378:metabolism
chemical_list: D003911:Dextrans; D005782:Gels; D006023:Glycoproteins; D006025:Glycosaminoglycans; D046911:Macromolecular Substances; D013431:Sulfates; D002807:Chondroitin; D003094:Collagen