Tryptophan metabolism and kynureninase induction in mutants of Neurospora crassa resistant to 4-methyl-tryptophan.
Mutants of Neurospora crassa that are resistant to 4-methyl-tryptophan were found to differ in ability to synthesize kynureninase in the presence of the inducers kynurenine, 3-OH-kynurenine, N-formyl-kynurenine, tryptophan, and indole. One strain (mtr26), although incapable of accumulating intracellular pools of these compounds, showed induced synthesis of kynureninase, whereas the second (mtr21) could neither accumulate nor be induced by them. Strain mtr21, with the suppressor su(mtr), could not be induced by indole but was induced by tryptophan and kynurenine derivatives. These results suggest that the mtr mutation, in addition to altering the ability of these strains to concentrate tryptophan and its metabolites, may have some effect on either the intracellular distribution of tryptophan or directly on the synthesis of kynureninase.
D001693:Biological Transport, Active; D002247:Carbon Isotopes; D002474:Cell-Free System; D004352:Drug Resistance, Microbial; D004790:Enzyme Induction; D006867:Hydrolases / Q000096:biosynthesis*; D007211:Indoles / Q000378:metabolism; D007737:Kynurenine / Q000378:metabolism; D008745:Methylation; D009154:Mutation; D009491:Neurospora / Q000187:drug effects*; D009492:Neurospora crassa / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D014364:Tryptophan / Q000378:metabolism* / Q000494:pharmacology; D062367:ortho-Aminobenzoates / Q000096:biosynthesis
D002247:Carbon Isotopes; D007211:Indoles; D062367:ortho-Aminobenzoates; D007737:Kynurenine; D014364:Tryptophan; D006867:Hydrolases