Mercurial-induced transformation of myosin prevented by adenosine triphosphate and pyrophosphate.
Adenosine triphosphate and pyrophosphate prevent the loss of Ca(//)-activated adenosine triphosphatase activity caused by high concentrations of mercurial sulfhydryl reagent. They concomitantly prevent the transformation of myosin into faster-sedimenting products. This is adduced as support for the hypothesis that the strategic sulfhydryl group is not binding adenosine triphosphate at the active site, but is initiating a conformational change upon its reaction with the mercurial reagent.
D000251:Adenosine Triphosphatases; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D002118:Calcium; D055598:Chemical Phenomena; D002621:Chemistry; D002729:Chloromercuribenzoates / Q000494:pharmacology*; D011756:Diphosphates / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D008628:Mercury / Q000494:pharmacology*; D009124:Muscle Proteins
D002729:Chloromercuribenzoates; D011756:Diphosphates; D009124:Muscle Proteins; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D008628:Mercury; D002118:Calcium