Effects of concanavalin A on mouse peritoneal macrophages. I. Stimulation of endocytic activity and inhibition of phago-lysosome formation.
Concanavalin A (Con A) binds to saccharide residues on the mouse peritoneal macrophage plasma membrane and stimulates extensive pinocytic interiorization of the membrane. The overall pinocytic rate is increased 3.5-4.5 times by the addition of Con A, and the surface marker enzyme adenosine triphosphatase can be identified histochemically in association with the cytoplasmic vesicles generated after exposure of the cells to Con A. Once formed, these pinocytic vesicles may persist for several days and fail to show morphologic evidence of fusion with primary or preformed secondary lysosomes. There is no apparent effect on the capacity of the macrophage to ingest either latex particles or IgG-coated SRBC administered either simultaneously with or subsequent to the Con A.
D000135:Acid Phosphatase / Q000032:analysis; D000251:Adenosine Triphosphatases; D000818:Animals; D000941:Antigens; D001665:Binding Sites; D002462:Cell Membrane / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology*; D004705:Endocytosis / Q000187:drug effects*; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D007840:Latex; D008247:Lysosomes / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D008863:Microspheres; D009708:Nucleotidases / Q000032:analysis; D010537:Peritoneum / Q000166:cytology; D010544:Peroxidases / Q000494:pharmacology; D010586:Phagocytes / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects*; D010873:Pinocytosis / Q000187:drug effects; D012756:Sheep / Q000276:immunology; D013268:Stimulation, Chemical; D014617:Vacuoles
D000941:Antigens; D007840:Latex; D003208:Concanavalin A; D010544:Peroxidases; D009708:Nucleotidases; D000135:Acid Phosphatase; D000251:Adenosine Triphosphatases