Source of energy for the Escherichia coli galactose transport systems induced by galactose.
The beta-methyl-galactoside- and galactose-specific transport systems of Escherichia coli were shown by experiments involving inhibitors and the use of an adenosine triphosphatase mutant strain to utilize adenosine 5'-triphosphate or a related compound to drive active transport. These systems were shown to be unable to use the activated-membrane state. The galactose-specific transport system was shown to behave most like a member of the binding-protein class of transport systems by its response to osmotic shock and vesicle formation. These results extended to two sugar transport systems: the correlation between the source of energy and class of transport system found by Berger (1973) for amino acid transport systems. That is, binding-protein systems utilized adenosine 5'-triphosphate whereas membrane-bound systems utilized the activated-membrane state to drive active transport.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D001149:Arsenates / Q000494:pharmacology; D001426:Bacterial Proteins / Q000378:metabolism; D001693:Biological Transport, Active; D002250:Carbon Radioisotopes; D004735:Energy Transfer; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D005632:Fructose / Q000378:metabolism; D005690:Galactose / Q000378:metabolism*; D009154:Mutation; D010085:Oxidative Phosphorylation / Q000187:drug effects; D011485:Protein Binding / Q000187:drug effects
D001149:Arsenates; D001426:Bacterial Proteins; D002250:Carbon Radioisotopes; D005632:Fructose; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D005690:Galactose