Different arginine transfer ribonucleic acid species prevalent in shaken and unshaken cultures of Neurospora.
When the arginyl-transfer ribonucleic acid (tRNA) species isolated from unshaken and from shaken cultures of Neurospora were compared by co-chromatography, a marked change in the relative abundance of the two main tRNA(arg) species was found. The two arginine tRNA species had different codon responses in ribosome binding assays. The tRNA(arg) eluting first (prevalent in shaken cultures) bound strongly to polyadenylic-guanylic acid [poly(A,G)] and to a lesser extent to polycytidylic-guanylic-adenylic acid [poly(C,G,A)]. The second tRNA(arg) species (prevalent in unshaken cultures) bound to poly(C,G,A) but not to poly(A, G). The possible significance of these observations is briefly discussed. Several modifications that improve the yield of tRNA from Neurospora were introduced in a standard isolation procedure.
D000596:Amino Acids / Q000378:metabolism; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D001120:Arginine / Q000096:biosynthesis / Q000378:metabolism; D001431:Bacteriological Techniques; D002247:Carbon Isotopes; D002474:Cell-Free System; D002845:Chromatography; D005815:Genetic Code; D009154:Mutation; D009491:Neurospora / Q000032:analysis*; D009492:Neurospora crassa / Q000032:analysis / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D011119:Polynucleotides; D014176:Protein Biosynthesis; D012343:RNA, Transfer / Q000032:analysis* / Q000302:isolation & purification; D012270:Ribosomes / Q000378:metabolism; D013698:Templates, Genetic; D014316:Tritium; D014461:Ultracentrifugation; D014732:Vibration
D000596:Amino Acids; D002247:Carbon Isotopes; D011119:Polynucleotides; D014316:Tritium; D012343:RNA, Transfer; D001120:Arginine; D000604:Amino Acyl-tRNA Synthetases