title: Cell wall alterations associated with the hyperproduction of extracellular enzymes in Neurospora crassa.
abstract: A pleiotropic mutation in Neurospora (exo-1), which confers derepression of alpha-amylase, glucoamylase, beta-fructofuranosidase, and trehalase, appears to also affect the composition of the cell wall. Segregants resulting from the backcross of exo-1 to the wild-type strain from which it derived are altered in the ratio of galactosamine to glucosamine in hydrolysates of isolated cell walls. Conidial cell walls exhibit a marked decrease in the amount of galactosamine in both exo-1 and exo-1(+) strains. Increased levels (approximately sevenfold) of amylase are found in conidia of exo-1, as compared with those of exo-1(+).
mesh_terms: D000681:Amylases / Q000096:biosynthesis*; D002473:Cell Wall / Q000032:analysis / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D003433:Crosses, Genetic; D005688:Galactosamine / Q000032:analysis; D005827:Genetics, Microbial; D005944:Glucosamine / Q000032:analysis; D006026:Glycoside Hydrolases / Q000096:biosynthesis*; D009154:Mutation; D009491:Neurospora / Q000201:enzymology*; D009492:Neurospora crassa / Q000032:analysis / Q000201:enzymology / Q000378:metabolism; D013172:Spores, Fungal / Q000032:analysis; D013393:Sucrase / Q000096:biosynthesis*; D014198:Trehalase / Q000096:biosynthesis
chemical_list: D005688:Galactosamine; D000681:Amylases; D006026:Glycoside Hydrolases; D014198:Trehalase; D013393:Sucrase; D005944:Glucosamine