Effects of an antibody to a highly purified Na+, K+-ATPase from canine renal medulla: separation of the "holoenzyme antibody" into catalytic and cardiac glycoside receptor-specific components.
An antiserum was prepared against a highly purified Na(+), K(+)-adenosine triphosphatase (ATP phosphohydrolase, EC 3.6.1.3). Purification and fractionation yielded two globulin components, one of which appears specific for a digitalis glycoside receptor site or binding conformation and the other for a catalytic site or conformation.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000936:Antigen-Antibody Complex; D001665:Binding Sites; D001666:Binding Sites, Antibody; D004285:Dogs; D007106:Immune Sera; D007668:Kidney / Q000201:enzymology*; D007679:Kidney Medulla / Q000201:enzymology; D010042:Ouabain / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D011485:Protein Binding; D011817:Rabbits / Q000276:immunology; D012964:Sodium / Q000378:metabolism; D014316:Tritium
D000906:Antibodies; D000936:Antigen-Antibody Complex; D007106:Immune Sera; D014316:Tritium; D010042:Ouabain; D012964:Sodium; D000251:Adenosine Triphosphatases; D011188:Potassium