Cytochalasin B: effects on cell morphology, cell adhesion, and mucopolysaccharide synthesis (cultured cells-contractile microfilaments-glycoproteins-embryonic cells-sorting-out).
Cytochalasin B reversibly causes extensive branching of myoblasts, fibroblasts, and nonencapsulated chondroblasts; it does not induce the formation of similar processes in myotubes, erythrocytes, amnion cells, encapsulated chondroblasts, or HeLa cells. The drug has no effect on the spontaneous contractions of isolated skeletal, cardiac, or smooth-muscle cells. Within 60 min, it depresses the incorporation of [(14)C]glucosamine into total mucopolysaccharide and glycoproteins by over 50%. The drug interferes with adhesion and sorting-out of dissociated embryonic cells. Cytochalasin B is likely to produce changes in components of the cell surface whose function is not readily or solely related to a system of "primitive contractile microfilaments."
D000650:Amnion / Q000166:cytology; D000818:Animals; D002247:Carbon Isotopes; D002448:Cell Adhesion / Q000187:drug effects*; D002478:Cells, Cultured; D002642:Chick Embryo; D003238:Connective Tissue / Q000187:drug effects*; D003239:Connective Tissue Cells; D004912:Erythrocytes / Q000187:drug effects; D005944:Glucosamine / Q000494:pharmacology; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006367:HeLa Cells / Q000187:drug effects; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000166:cytology; D009183:Mycotoxins / Q000494:pharmacology*
D002247:Carbon Isotopes; D006025:Glycosaminoglycans; D009183:Mycotoxins; D005944:Glucosamine