The deoxyfluoro-D-glucopyranose 6-phosphates and their effect on yeast glucose phosphate isomerase.
1. The deoxyfluoro-d-glucopyranose 6-phosphates were prepared from the corresponding deoxyfluoro-d-glucoses and ATP by using hexokinase. 2. 3-Deoxy-3-fluoro- and 4-deoxy-4-fluoro-d-glucose 6-phosphate were substrates for glucose phosphate isomerase, and in addition the products of this reaction, 3-deoxy-3-fluoro- and 4-deoxy-4-fluoro-d-fructose 6-phosphate respectively, were good substrates for phosphofructokinase. 3. Some C-2-substituted derivatives of d-glucose 6-phosphate were found to be competitive inhibitors of glucose phosphate isomerase. 4. The possible role of the hydroxyl groups in the binding of d-glucose 6-phopshate to glucose phosphate isomerase is discussed.
D003201:Computers; D004590:Electrophoresis, Paper; D005461:Fluorine / Q000494:pharmacology*; D005956:Glucose-6-Phosphate Isomerase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005958:Glucosephosphates / Q000138:chemical synthesis / Q000494:pharmacology*; D006593:Hexokinase; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009904:Optical Rotation; D010732:Phosphofructokinase-1; D011868:Radioisotopes; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D005958:Glucosephosphates; D011868:Radioisotopes; D005461:Fluorine; D006593:Hexokinase; D010732:Phosphofructokinase-1; D005956:Glucose-6-Phosphate Isomerase