Differentiation of thymus-derived cells from precursors in mouse bone marrow.
An experimental model system was developed to study the differentiation of thymus-derived (T) cells from progenitors in bone marrow. In this system transplantation of bone marrow cells depleted of T cells gave rise to T cells in the spleens of irradiated recipients within 15 days of transplantation. Thus, normal bone marrow contains a class of cells that are progeniotrs of T cells (PT cells). PT cells are different from T cells since PT cells are incapable of responding to alloantigen, are theta resistant, and band in a lighter density region than do T cells. The density profile of PT cells is different from that of hematopoietic stem cells (CFU-S); PT cells band in a denser region of the gradient than do CFU-S.
D000818:Animals; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002454:Cell Differentiation; D002499:Centrifugation, Density Gradient; D002859:Chromium Isotopes; D003165:Complement System Proteins; D003601:Cytotoxicity Tests, Immunologic; D006412:Hematopoietic Stem Cells; D007106:Immune Sera; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D051379:Mice; D011134:Polysaccharides; D011830:Radiation Effects; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000528:radiation effects; D013395:Sucrose; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D014184:Transplantation, Homologous
D002859:Chromium Isotopes; D007106:Immune Sera; D011134:Polysaccharides; D013395:Sucrose; D003165:Complement System Proteins