Studies on in vitro DNA synthesis. Isolation of DNA B gene product from Escherichia coli.
Formation of duplex DNA from varphiX174 single-stranded DNA by extracts of E. coli was previously shown to require the gene product of dna B. Using as an assay the stimulation of varphiX174 DNA-dependent synthesis in inactivated extracts of dna B temperature-sensitive cells, we purified the dna B gene product from wild-type E. coli as well as from a dna B temperature-sensitive mutant. The dna B temperature-sensitive gene product is more thermolabile than its wild-type counterpart.
D000251:Adenosine Triphosphatases / Q000302:isolation & purification* / Q000378:metabolism; D000645:Ammonium Sulfate; D002498:Centrifugation; D002499:Centrifugation, Density Gradient; D002848:Chromatography, DEAE-Cellulose; D003090:Coliphages; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004277:DNA, Single-Stranded / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology*; D005796:Genes; D006358:Hot Temperature; D008970:Molecular Weight; D009154:Mutation; D011489:Protein Denaturation; D012293:Rifampin / Q000494:pharmacology
D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D000251:Adenosine Triphosphatases; D000645:Ammonium Sulfate; D012293:Rifampin