Use of neomycin in the isolation of mutants blocked in energy conservation in Escherichia coli.
A neomycin-resistant mutant of Escherichia coli K-12 unable to grow on Krebs cycle intermediates has been isolated. The mutant retained its respiratory capacity, but lacked membrane Mg(2+)-Ca(2+)-stimulated adenosine triphosphatase activity (EC 3.6.1.3).
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000332:Aerobiosis; D000693:Anaerobiosis; D002118:Calcium / Q000494:pharmacology; D002462:Cell Membrane / Q000201:enzymology; D002474:Cell-Free System; D003470:Culture Media; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007656:Ketoglutaric Acids / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D008293:Malates / Q000378:metabolism; D009154:Mutation; D009355:Neomycin / Q000494:pharmacology*; D009566:Nitrates / Q000378:metabolism; D010101:Oxygen Consumption; D013386:Succinates / Q000378:metabolism
D003470:Culture Media; D007656:Ketoglutaric Acids; D008293:Malates; D009566:Nitrates; D013386:Succinates; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D009355:Neomycin; D008274:Magnesium; D005947:Glucose; D002118:Calcium