title: On the similarity between the tRNAs of organelles and prokaryotes.
abstract: Fluorescence studies with organelle transfer RNAs separated from their cytoplasmic counterparts revealed that phenylalanine tRNA from Euglena chloroplasts or Neurospora mitochondria does not contain a fluorescent "base Y." In contrast, cytoplasmic phenylalanine tRNA from Euglena and cytoplasmic tRNA from Neurospora were found to contain fluorescent bases. The fluorescence-emission spectra of Neurospora cytoplasmic tRNAs and those of the related ascomycete Saccharomyces cerevisiue were observed to be quite different. The results support the generalization that eukaryotic tRNAs contain a fluorescent base, but indicate that their respective organelle tRNAs do not. They also indicate a striking parallelism between organelle and prokaryotic tRNAs.
mesh_terms: D000215:Acylation; D002736:Chloroplasts / Q000032:analysis*; D002845:Chromatography; D002848:Chromatography, DEAE-Cellulose; D003593:Cytoplasm / Q000032:analysis*; D005056:Euglena gracilis / Q000032:analysis / Q000166:cytology*; D005453:Fluorescence; D008928:Mitochondria / Q000032:analysis*; D009491:Neurospora / Q000166:cytology*; D009492:Neurospora crassa / Q000166:cytology; D010649:Phenylalanine / Q000096:biosynthesis / Q000378:metabolism; D010788:Photosynthesis; D012343:RNA, Transfer / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000166:cytology; D014316:Tritium
chemical_list: D014316:Tritium; D010649:Phenylalanine; D012343:RNA, Transfer