title: Base ratio and deoxyribonucleic acid homology studies of six Staphylococcus aureus typing bacteriophages.
abstract: Genetic relatedness among Staphylococcus aureus typing bacteriophages 80, 47, 81, 71, 77, and 187 was investigated by using base ratio determinations and deoxyribonucleic acid (DNA)-DNA hybridization. Guanine/cytosine (G/C) content, as determined by thermal denaturation and chromatographic analysis of the purines released by acid hydrolysis of the DNA, was between 31 and 36%. No pattern correlating G/C content with serological or lytic group was discernible. DNA-DNA hybridization studies indicated high degrees of homology (43% or more) among the genomes of phages in the same serological group. Less homology (29% or less) was observed between the genomes of phages belonging to different serological groups. These findings implied a positive correlation between serological and genetic relatedness.
mesh_terms: D001434:Bacteriophage Typing; D002845:Chromatography; D003596:Cytosine / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D006147:Guanine / Q000032:analysis*; D006358:Hot Temperature; D008242:Lysogeny; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D012703:Serotyping; D013210:Staphylococcus / Q000145:classification; D013204:Staphylococcus Phages / Q000032:analysis*
chemical_list: D004279:DNA, Viral; D006147:Guanine; D003596:Cytosine