Establishment of four functional, clonal strains of animal cells in culture.
The single-cell plating technique was used to develop four clonal cell lines that perform organ-specific functions after being serially cultured for prolonged periods. These strains include steroid-secreting Leydig cells, melanomacells that form pigment, and two strains from a hormone-secreting rat pituitary tumor. One of the cell lines from the pituitary tumor secretes growt hormone, while another line derived from the same tumor secretes a substance similar to adrenocorticotropic hormone.
D000324:Adrenocorticotropic Hormone / Q000096:biosynthesis*; D000818:Animals; D002999:Clone Cells; D046508:Culture Techniques; D013006:Growth Hormone / Q000096:biosynthesis*; D007984:Leydig Cell Tumor; D007985:Leydig Cells; D008297:Male; D008545:Melanoma; D009374:Neoplasms, Experimental; D010911:Pituitary Neoplasms; D051381:Rats
D000324:Adrenocorticotropic Hormone; D013006:Growth Hormone