Production of high-titer antibody in serum and ascitic fluid of hamsters for a variety of virus-induced tumor antigens.
Methods not involving the use of viable tumors were developed for production of high-titer antibodies. The methods involved use of tumor homogenates. Homogenates from all tumor lines tested contained the characteristic tumor antigen. Attempts to isolate virus failed, and virus antigens could not be demonstrated. Immunization with tumor homogenates, in Freund's complete adjuvant, resulted in high antibody levels. None of the animals so immunized developed tumors.
D000818:Animals; D000912:Antibodies, Neoplasm / Q000032:analysis* / Q000097:blood; D000914:Antibodies, Viral / Q000032:analysis* / Q000097:blood; D000957:Antigens, Viral, Tumor / Q000276:immunology*; D001201:Ascites / Q000276:immunology*; D003168:Complement Fixation Tests; D006224:Cricetinae; D006801:Humans; D009374:Neoplasms, Experimental / Q000276:immunology*; D009858:Oncogenic Viruses / Q000276:immunology*; D011120:Polyomavirus / Q000276:immunology; D001357:Sarcoma, Avian / Q000276:immunology; D013539:Simian virus 40 / Q000276:immunology
D000912:Antibodies, Neoplasm; D000914:Antibodies, Viral; D000957:Antigens, Viral, Tumor