Dry-column chromatographic isolation of fatty acid esters of phorbol from croton oil.
The family of mixed fatty acid esters of 4,9,12,13,20-pentahydroxy-tiglia-1,6-dien-3-one (phorbol) has been isolated from croton oil by column chromatography on silica gel and preparative dry-column chromatography on silica gel HF/254 packed in a cellophane membrane. The tumorpromoting agents are obtained rapidly and with minimum difficulty.
D000438:Alcohols; D001952:Bridged-Ring Compounds; D002845:Chromatography; D003436:Croton Oil / Q000032:analysis*; D004952:Esters / Q000302:isolation & purification; D005227:Fatty Acids / Q000302:isolation & purification*; D005782:Gels; D007659:Ketones; D008722:Methods; D012822:Silicon Dioxide
D000438:Alcohols; D001952:Bridged-Ring Compounds; D004952:Esters; D005227:Fatty Acids; D005782:Gels; D007659:Ketones; D012822:Silicon Dioxide; D003436:Croton Oil