title: Human diploid cell transformation by DNA extracted from the tumor virus SV40.
abstract: The DNA isolated from simian virus 40 (SV40) can transform human fibroblast cells in tissue culture. Clonal lines of DNA-transformed human cells have been obtained that have properties characteristic of cells transformed by whole virus. They all contain SV40 T-antigen, and infectious virus can be recovered by cocultivation. This is the first demonstration of a permanent genetic alteration produced in human cells by purified DNA.
mesh_terms: D000818:Animals; D000941:Antigens / Q000032:analysis; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells; D046508:Culture Techniques; D004279:DNA, Viral; D005347:Fibroblasts; D006801:Humans; D009374:Neoplasms, Experimental / Q000276:immunology*; D013539:Simian virus 40 / Q000276:immunology*
chemical_list: D000941:Antigens; D004279:DNA, Viral