title: Isolation and identification of phospholipids of bovine rhodopsin.
abstract: Phospholipids present in digitonin solutions of bovine rhodopsin have been identified and assayed. Digitonin interferes with extraction of lipids by the usual methods; digitonin was therefore removed from the preparation as an ergosterol digitonide, soluble in absolute ethanol but precipitated in 80% ethanol. The supernatant 80% ethanol contained one portion of the phospholipid, mainly choline and ethanolamine phosphoglycerides with traces of serine phosphoglyceride and sphingomyelin. The rhodopsin residue (free from digitonin) was extracted with chloroform-methanol 2:1; this extract contained the rest of the phospholipid, which consisted only of choline and ethanolamine phosphoglycerides. Plasmalogens were not found, but could have decomposed during the procedures.
mesh_terms: D000818:Animals; D002417:Cattle; D011232:Chemical Precipitation; D002855:Chromatography, Thin Layer; D004071:Digitalis Glycosides; D000431:Ethanol; D005612:Freeze Drying; D008722:Methods; D010713:Phosphatidylcholines / Q000032:analysis; D010714:Phosphatidylethanolamines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis* / Q000302:isolation & purification; D010758:Phosphorus / Q000032:analysis; D010786:Photoreceptor Cells / Q000032:analysis; D010955:Plasmalogens / Q000032:analysis; D012160:Retina / Q000166:cytology; D012168:Retinal Pigments / Q000032:analysis*; D012822:Silicon Dioxide; D012995:Solubility; D013109:Sphingomyelins / Q000032:analysis; D014807:Vitamin D
chemical_list: D004071:Digitalis Glycosides; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D010955:Plasmalogens; D012168:Retinal Pigments; D013109:Sphingomyelins; D014807:Vitamin D; D010758:Phosphorus; D000431:Ethanol; D012822:Silicon Dioxide