Characterization of intracellular ribonucleic acid specific for the murine sarcoma-leukemia virus complex.
Virus particles were continuously produced by a cell line (78A1) of rat embryo fibroblasts that had been transformed by the murine sarcoma-leukemia virus complex. Since most of the mature virions were found in the extracellular fluid and were not cell-associated, a measurable quantity of viral ribonucleic acid (RNA) could not be extracted from these cells. Cycloheximide, a protein inhibitor, was successfully used to accumulate viral RNA within the cells. This ribonuclease-sensitive RNA, with a sedimentation coefficient of 71S, had the same base composition as the high molecular weight RNA (S(20,w) = 71) isolated from purified virions released by the transformed cells.
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D003673:Defective Viruses; D004622:Embryo, Mammalian; D005347:Fibroblasts; D017988:Gammaretrovirus / Q000254:growth & development; D006378:Helper Viruses; D009052:Leukemia Virus, Murine / Q000254:growth & development; D008854:Microscopy, Electron; D008970:Molecular Weight; D010759:Phosphorus Isotopes; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats; D012260:Ribonucleases / Q000494:pharmacology; D012513:Sarcoma, Experimental; D014779:Virus Replication / Q000187:drug effects
D010759:Phosphorus Isotopes; D012367:RNA, Viral; D003513:Cycloheximide; D012260:Ribonucleases