title: A nondefective (competent) adenovirus-SV40 hybrid isolated from the AD.2-SV40 hybrid population.
abstract: A new nondefective hybrid virus has been plaque-isolated from the Ad.2-SV40 hybrid population. This virus replicates efficiently with one-hit kinetics in both human embryonic kidney and African green monkey kidney cells, induces an SV40 specific antigen which is detectable by immunofluorescence and complement-fixation using sera from SV40 tumor-bearing hamsters, and produces SV40-specific RNA detectable by DNA-RNA hybridization. The SV40-specific antigen induced by this virus is heat-stable, sensitive to inhibitors of DNA synthesis, serologically different from SV40 T and viral antigens, and is an unrecognized SV40 antigen.
mesh_terms: D000256:Adenoviridae / Q000302:isolation & purification*; D000818:Animals; D000941:Antigens / Q000032:analysis; D005455:Fluorescent Antibody Technique; D000882:Haplorhini; D006801:Humans; D006824:Hybridization, Genetic; D007668:Kidney; D013539:Simian virus 40 / Q000302:isolation & purification*; D014776:Virus Cultivation
chemical_list: D000941:Antigens