Propagation of MM virus in L cells.
MM virus (mouse-brain stock) replicated to a limited extent in L cells without cytopathic effects; the average yield was less than 1 plaque-forming unit/cell. Passage in BHK-21 cells resulted in MM virus [MM(BHK)] which replicated to high titers (200 to 300 plaque-forming units/cell) in L cells with complete cytopathic effects. Appearance of mature MM(BHK) virus in L-cell cultures begins 4 hr after infection and is completed by 8 hr. Release of mature virus was slow (less than 1% at 8 hr) but was completed by 24 hr.
D000220:Adaptation, Biological; D003588:Cytopathogenic Effect, Viral; D004686:Encephalomyocarditis virus / Q000302:isolation & purification*; D007739:L Cells; D008722:Methods; D014776:Virus Cultivation; D014779:Virus Replication