Isolation of deoxyribonucleic acid from mammalian tissues.
1. DNA has been isolated from different mammalian tissues. The DNA preparations were free from RNA, protein and polysaccharides and have a similar range of sedimentation coefficients (approx. 24s). 2. Protein was removed by a two-stage extraction with a phenol-cresol mixture by using a detergent with 4-aminosalicylate in the first stage and sodium chloride in the second. 3. Polysaccharides remained in solution when DNA was precipitated with 2-butoxyethanol in the presence of 0.5m-sodium chloride and 1.5m-sodium benzoate. 4. Ribosomal RNA was removed by precipitation in the presence of 3m-sodium chloride at 0 degrees , when DNA remained soluble.
D000818:Animals; D006528:Carcinoma, Hepatocellular; D002498:Centrifugation; D004247:DNA / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis*; D006358:Hot Temperature; D066298:In Vitro Techniques; D008099:Liver / Q000032:analysis*; D008113:Liver Neoplasms; D009691:Nucleic Acid Denaturation; D012313:RNA / Q000032:analysis; D051381:Rats; D012270:Ribosomes / Q000032:analysis; D012513:Sarcoma, Experimental; D013154:Spleen / Q000032:analysis*
D004273:DNA, Neoplasm; D012313:RNA; D004247:DNA