title: Diphtheria toxin subunit active in vitro.
abstract: Exposure of diphtheria toxin to dithiothreitol (and similar thiols) resulted in a subunit which was active in catalyzing the adenosine diphosphateribosylation of mammalian aminoacyl-transferase II in the presence of nicotinamide adenine dinucleotide. At the same time there was a marked increase in total ADP-ribosylation activity. A molecule which was apparently identical to the derived subunit in size and activity was detected in partially purified preparations of toxin.
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism; D002384:Catalysis; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D004167:Diphtheria Toxin / Q000032:analysis / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D009243:NAD / Q000378:metabolism; D013438:Sulfhydryl Compounds
chemical_list: D000227:Adenine Nucleotides; D004167:Diphtheria Toxin; D013438:Sulfhydryl Compounds; D009243:NAD