Biochemical basis of obligate autotrophy in Nitrosomonas europaea.
The specific activities of isocitric dehydrogenase, alpha-ketoglutaric dehydrogenase, succinic dehydrogenase, malic dehydrogenase, and reduced nicotinamide adenine dinucleotide (NADH) oxidase were determined in extracts of Nitrosomonas europaea and compared with the corresponding values for Anacystis nidulans and autotrophically grown Hydrogenomonas eutropha. In common with other obligate autotrophs and in contrast to facultative autotrophs, Nitrosomonas extracts lacked alpha-ketoglutaric dehydrogenase and KCN-sensitive NADH oxidase activity and had low succinic dehydrogenase activity. The Nitrosomonas NADH oxidase appeared to be of the peroxidase type.
D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D007656:Ketoglutaric Acids; D008291:Malate Dehydrogenase / Q000378:metabolism; D009243:NAD; D009606:Nitrosomonas / Q000201:enzymology* / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000378:metabolism
D007656:Ketoglutaric Acids; D009243:NAD; D010088:Oxidoreductases; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D013385:Succinate Dehydrogenase