Effect of poxvirus infection on host cell deoxyribonucleic acid synthesis.
Deoxyribonucleic acid (DNA) synthesis was studied in poxvirus-infected cells by measuring (14)C-thymidine incorporation into viral and host cell DNA. A complete separation of the two species of DNA was achieved by combining the previously used "Dounce method" with a separation method based on different reannealing properties of viral and vertebrate DNA. Shortly after infection of HeLa cells with poxviruses, a burst of viral DNA synthesis occurred in the cytoplasm, but a rapid inhibition of host-cell DNA synthesis in the nucleus was observed. This inhibition of cellular DNA synthesis was also found if an accumulation of viral DNA was prevented. At high multiplicites, ultraviolet-irradiated virus inhibited host-cell DNA synthesis to the same extent as fully infectious poxvirus. Under the same conditions, heating at 60 C for 15 min caused a decrease in the ability of cowpox virus to inhibit host-cell DNA synthesis, but did not produce the same effect on vaccinia virus strain WR.
D002247:Carbon Isotopes; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004247:DNA / Q000032:analysis / Q000096:biosynthesis*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006367:HeLa Cells; D006358:Hot Temperature; D006801:Humans; D008722:Methods; D011212:Poxviridae / Q000528:radiation effects; D011213:Poxviridae Infections / Q000378:metabolism*; D011830:Radiation Effects; D013936:Thymidine / Q000378:metabolism; D014466:Ultraviolet Rays
D002247:Carbon Isotopes; D004279:DNA, Viral; D004247:DNA; D013936:Thymidine