title: Electron microscopic study of the morphogenesis of vesicular stomatitis virus.
abstract: Except for the rate, vesicular stomatitis virus (VSV) grows as well at 25 C as at 37 C in primary chick embryo fibroblast cells and in a pig kidney cell line [PK(H13)]. Maximal yields were reached at about 28 hr at 25 C and 10 hr at 37 C in these cells. Morphogenesis, as observed by electron microscopy, was similar at the two temperatures. The main feature was accumulation of virus in intracytoplasmic vacuoles. Mode of release of VSV has been controversial; both budding (as displayed by myxoviruses) and maturation at membranes of cytoplasmic vacuoles (as with arboviruses) have been claimed. Our observations support the latter view, and the apparent dichotomy in interpretation is discussed.
mesh_terms: D000818:Animals; D046508:Culture Techniques; D003593:Cytoplasm; D008854:Microscopy, Electron; D009024:Morphogenesis; D013696:Temperature; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development*