Enzymatic synthesis of cytidine diphosphate diglyceride.
Evidence is presented for the enzymatic formation of cytidine diphosphate diglyceride in microsomal preparations from guinea pig liver according to the reaction: CTP + phosphatidic acid right harpoon over left harpoon CDP-diglyceride + p-O-P. Conditions have been found in which the incorporation of labeled CTP into CDP-diglyceride is almost entirely dependent upon added phosphatidic acid. The incorporation of CMP into lipid is very slight. A substantial net synthesis of CDP-diglyceride takes place under these conditions. Some properties of the enzyme system are described.
D000818:Animals; D003597:Cytosine Nucleotides / Q000096:biosynthesis*; D011756:Diphosphates / Q000494:pharmacology; D005989:Glycerides / Q000096:biosynthesis*; D006168:Guinea Pigs; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D014316:Tritium
D003597:Cytosine Nucleotides; D011756:Diphosphates; D005989:Glycerides; D014316:Tritium; D008274:Magnesium