Rescue of temperature-sensitive poliovirus.
A temperature-sensitive strain of type 1 poliovirus, LSc, was functionally rescued when infected cells were incubated at 40 C in the presence of Mahoney, a temperature-resistant strain of type 1 poliovirus. The rescue value was 9% of the mutant yield obtained under permissive conditions. Rescued virus underwent replication, because the progeny of (32)P-labeled LSc were not radiosensitive. Serum inactivation studies with Mahoney specific antiserum indicated that a small amount of phenotypic mixing occurred among the rescued particles. The temperature-sensitive event occurred between 2 and 4 hr postinfection in the developmental cycle of LSc. Neither viral polymerase activity nor virus-induced ribonucleic acid could be demonstrated in infected cells between 2 and 4 hr after infection at 40 C with the temperature-sensitive mutant.
D000418:Albumins; D000941:Antigens; D002845:Chromatography; D005260:Female; D006367:HeLa Cells; D006378:Helper Viruses; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D009154:Mutation; D009500:Neutralization Tests; D009713:Nucleotidyltransferases / Q000378:metabolism; D010641:Phenotype; D010759:Phosphorus Isotopes; D017955:Poliovirus / Q000201:enzymology; D013696:Temperature; D014779:Virus Replication
D000418:Albumins; D000941:Antigens; D007106:Immune Sera; D010759:Phosphorus Isotopes; D009713:Nucleotidyltransferases