A study of the heterogeneous 37S ribonucleic acid induced by foot-and-mouth-disease virus.
1. The 37s RNA induced in baby-hamster kidney cells by infection with foot-and-mouth-disease virus was examined on sucrose gradients and by filtration through Sepharose 4B. 2. The RNA sedimented faster (37s) and as a broader band than the 35s RNA from purified virus. 3. Treatment with deoxyribonuclease, Pronase or amylase did not alter the sedimentation profile of the 37s RNA. 4. Treatment of individual fractions of the RNA with phenol, dimethyl sulphoxide or methylCellosolve did not decrease the sedimentation rate of the faster-sedimenting molecules. 5. Sedimentation in sucrose gradients of different ionic strengths or containing EDTA had no effect on the heterogeneous nature of the profile. 6. On filtration through Sepharose 4B columns, the 37s virus-induced RNA was eluted before viral RNA. 7. Only 20% of the rapidly sedimenting RNA was incorporated into complete virus particles.
D000818:Animals; D005537:Aphthovirus / Q000378:metabolism; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D002999:Clone Cells / Q000378:metabolism* / Q000382:microbiology; D006224:Cricetinae; D046508:Culture Techniques; D007668:Kidney / Q000166:cytology*; D010710:Phosphates / Q000378:metabolism; D010759:Phosphorus Isotopes; D012313:RNA / Q000032:analysis / Q000096:biosynthesis*; D012367:RNA, Viral / Q000032:analysis; D013053:Spectrophotometry; D014529:Uridine / Q000378:metabolism; D014776:Virus Cultivation
D002247:Carbon Isotopes; D010710:Phosphates; D010759:Phosphorus Isotopes; D012367:RNA, Viral; D012313:RNA; D014529:Uridine