Uncoating of poliovirus by isolated plasma membranes.
Poliovirus type I LSc strain labeled with (14)C-uridine was adsorbed onto isolated plasma membranes and incubated with them. When membranes from Hep-2 or Vero cells were used, 22% of the label was converted to a trichloroacetic acid-soluble form, when trypsin or ribonuclease was added, the fraction rendered soluble was increased, and when the two enzymes were added in sequence, 85% or more of the label became trichloroacetic acid-soluble. This labilization of poliovirus could be reproduced when butanol-solubilized proteins from membranes were substituted for the whole plasma membranes, but it did not occur with membranes from polio-virus-resistant calf kidney or BHK-21 cells.
D000327:Adsorption; D000818:Animals; D002247:Carbon Isotopes; D002277:Carcinoma; D002417:Cattle; D002460:Cell Line; D002462:Cell Membrane; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D004622:Embryo, Mammalian; D000882:Haplorhini; D006801:Humans; D007668:Kidney; D007822:Laryngeal Neoplasms; D008854:Microscopy, Electron; D017955:Poliovirus; D012260:Ribonucleases; D012995:Solubility; D013395:Sucrose; D014238:Trichloroacetic Acid; D014357:Trypsin; D014529:Uridine
D002247:Carbon Isotopes; D013395:Sucrose; D014238:Trichloroacetic Acid; D012260:Ribonucleases; D014357:Trypsin; D014529:Uridine