The immunochemistry of Salmonella chemotype VI O-antigens. The structure of oligosaccharides from Salmonella group G (o 13,22) lipopolysaccharides.
1. Lipopolysaccharides have been isolated from ;smooth' (S) strains of Salmonella friedenau and Salmonella poona by the phenol-water method and purified in the preparative ultracentrifuge. 2. These lipopolysaccharides are serologically indistinguishable and on partial acid hydrolysis the same series of oligosaccharides was obtained in each instance. 3. The results of quantitative micro-analysis, borohydride reduction, periodate oxidation, Morgan-Elson reactions and enzymic hydrolysis with beta-galactosidase on the isolated oligosaccharides indicate that the O-specific side chains of these lipopolysaccharides have a repeating pentasaccharide unit that is beta-d-galactosyl-(1-->3)-N-acetylgalactosaminyl-(1-->3)-N-acetylgalactosaminyl-(1-->4)-l-fucose with a d-glucose residue bound at an undetermined point on this structure. 4. Two oligosaccharides, a glucosyl-galactose and an N-acetylglucosaminylglucose, have also been isolated and these seem to be identical with oligosaccharides obtained from ;rough' (R) Salmonella lipopolysaccharides. These findings are in accordance with the view that Salmonella S-lipopolysaccharides have a core that consists of R-lipopolysaccharide.
D000941:Antigens; D055598:Chemical Phenomena; D002621:Chemistry; D002854:Chromatography, Paper; D003168:Complement Fixation Tests; D005696:Galactosidases / Q000378:metabolism; D066298:In Vitro Techniques; D008070:Lipopolysaccharides; D009844:Oligosaccharides; D010504:Periodic Acid; D012475:Salmonella / Q000276:immunology*; D014461:Ultracentrifugation
D000941:Antigens; D008070:Lipopolysaccharides; D009844:Oligosaccharides; D010504:Periodic Acid; D005696:Galactosidases