Stimulated formation of adenosine 3',5'-cyclic phosphate in cerebral cortex: synergism between electrical activity and biogenic amines.
When cortical slices are incubated with adenine-(14)C, adenine nucleotides are labeled in a small and relatively stable pool. The ATP-(14)C of this pool is readily converted to cAMP-(14)C during incubations with depolarizing agents, such as K(+), ouabain, veratridine, or batrachotoxin. During incubations with these agents, release of acetylcholine and of adenosine into the medium is enhanced. The increase in release of adenosine parallels the enhanced formation of cAMP-(14)C elicited by depolarizing agents, providing further evidence that adenosine may serve to couple electrical activity in the central nervous system with formation of cAMP. When adenosine or a depolarizing agent are incubated, together with a biogenic amine, such as histamine, serotonin, or norepinephrine, the combined effect on cAMP-(14)C formation in cortical slices is much more than additive. Extracellular levels of biogenic amines could in this manner modulate cAMP formation and biochemical responses in nervous tissue during electrical activity.
D000109:Acetylcholine / Q000378:metabolism; D000227:Adenine Nucleotides / Q000096:biosynthesis*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002247:Carbon Isotopes; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D000242:Cyclic AMP / Q000096:biosynthesis; D004298:Dopamine / Q000494:pharmacology; D004594:Electrophysiology; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology*; D066298:In Vitro Techniques; D009638:Norepinephrine / Q000494:pharmacology*; D009647:Normetanephrine / Q000494:pharmacology; D009705:Nucleosides / Q000494:pharmacology; D010042:Ouabain / Q000494:pharmacology; D010627:Phenethylamines / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D012701:Serotonin / Q000494:pharmacology; D013268:Stimulation, Chemical; D014702:Veratrine / Q000494:pharmacology
D000227:Adenine Nucleotides; D002247:Carbon Isotopes; D009705:Nucleosides; D010627:Phenethylamines; D009647:Normetanephrine; D012701:Serotonin; D010042:Ouabain; D006632:Histamine; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D014702:Veratrine; D000109:Acetylcholine; D011188:Potassium; D002118:Calcium; D004298:Dopamine; D009638:Norepinephrine