Microneutralization test for determination of rhinovirus and coxsackievirus A antibody in human diploid cells.
A method for determination of serum-neutralizing antibody titers to rhinovirus type 16 and coxsackievirus type A-4 in human diploid cells (WI-38) grown in Microtiter plates is described. A good correlation was observed when comparing neutralization tests in WI-38 and other cells. The WI-38 Microtiter method is relatively simple and economical. It is suitable for those laboratories which are required to conduct large-scale serological evaluation of antibodies for rhinoviruses and coxsackie A viruses. This system also can be used for cytomegalovirus and varicella-zoster virus, which grow relatively slowly.
D000906:Antibodies / Q000032:analysis*; D002648:Child; D046508:Culture Techniques; D003587:Cytomegalovirus / Q000276:immunology; D004770:Enterovirus / Q000276:immunology*; D006367:HeLa Cells; D014645:Herpesvirus 3, Human / Q000276:immunology; D006801:Humans; D008722:Methods; D009500:Neutralization Tests; D012229:Rhinovirus / Q000276:immunology*
D000906:Antibodies