title: Carbon balance of a mannitol fermentation and the biosynthetic pathway.
abstract: The carbon balance was determined for a fermentation in which mannitol is produced from glucose by an Aspergillus species. The products found were: cells (17% of carbon input), CO(2) (26%), mannitol (35%), glycerol (10%), erythritol (2.5%), glycogen (1%), and unidentified compounds (8%). Thus, 92% of the carbon input was accounted for. Cell-free enzyme studies showed that mannitol was synthesized via the reduction of fructose-6-phosphate and not by the direct reduction of fructose. If the cell yield from glucose was assumed to be 50% and the theoretical conversion efficiency from glucose to polyols was 90%, as calculated from the energy balance, then 34% of the glucose carbon was used for growth and 53% was used for polyol formation.
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism; D001230:Aspergillus / Q000254:growth & development / Q000378:metabolism*; D002245:Carbon Dioxide / Q000096:biosynthesis; D002474:Cell-Free System; D004896:Erythritol / Q000096:biosynthesis; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D005990:Glycerol / Q000096:biosynthesis; D006003:Glycogen / Q000096:biosynthesis; D006600:Hexosephosphates / Q000378:metabolism; D008353:Mannitol / Q000096:biosynthesis*; D009243:NAD / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism
chemical_list: D006600:Hexosephosphates; D009243:NAD; D002245:Carbon Dioxide; D008353:Mannitol; D006003:Glycogen; D000429:Alcohol Oxidoreductases; D010744:Phosphoric Monoester Hydrolases; D005947:Glucose; D005990:Glycerol; D004896:Erythritol