The metabolism of D-glucarate by Pseudomonas acidovorans.
1. Dehydratases that converted d-glucarate into 4-deoxy-5-oxoglucarate were partially purified from Klebsiella aerogenes and Pseudomonas acidovorans. 2. When d-glucarate was metabolized to 2,5-dioxovalerate it appeared that water and carbon dioxide were removed from 4-deoxy-5-oxoglucarate in one enzymic step: 4,5-dihydroxy-2-oxovalerate was not an intermediate in this reaction. 3. A method for the enzymic determination of d-glucarate is described.
D000272:Adipates / Q000378:metabolism*; D002247:Carbon Isotopes; D002845:Chromatography; D004586:Electrophoresis; D007709:Klebsiella / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D011549:Pseudomonas / Q000378:metabolism*; D014631:Valerates / Q000378:metabolism
D000272:Adipates; D002247:Carbon Isotopes; D014631:Valerates; D009243:NAD