Survey of microorganism for the production of extracellular phytase.
A culture enrichment technique was used to isolate phytase-producing microorganisms. Also, microorganisms from various culture collections were tested for their phytase-producing ability. A number of the Aspergillus niger group produced extracellular phytase which dephosphorylated calcium phytate in acidic solution. A soil isolate, A. ficuum NRRL 3135, produced the most active phytase in a cornstarch-based medium. Production of phytase was strongly repressed by inorganic phosphates and required a high carbon to phosphorus ratio in the medium.
D010832:6-Phytase / Q000096:biosynthesis*; D001230:Aspergillus / Q000201:enzymology*; D001419:Bacteria / Q000201:enzymology; D004794:Enzyme Repression; D005658:Fungi / Q000201:enzymology; D010710:Phosphates / Q000494:pharmacology; D010758:Phosphorus / Q000494:pharmacology; D012988:Soil Microbiology; D013213:Starch / Q000494:pharmacology
D010710:Phosphates; D010758:Phosphorus; D013213:Starch; D010832:6-Phytase