A spectrophotometric method for the microdetermination of periodate.
1. A method is described for measuring the concentration of periodate over the range 0.2-20mum by adding 1,2-di-(p-dimethylaminophenyl)ethane-1,2-diol to a sample solution. Periodate cleaves this compound to from two molecules of p-dimethylaminobenzaldehyde, the extinction of which is then read at 352mmu. 2. The method has been used to follow the course of periodate oxidations of serine methyl ester, ribonuclease A and ribonuclease S-protein. Addition of the reagent stops further periodate reaction by reducing the remaining periodate to iodate. 3. The presence of protein does not interfere with the assay.
D000438:Alcohols; D000814:Aniline Compounds; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008722:Methods; D008832:Microchemistry; D008956:Models, Chemical; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000032:analysis*; D011506:Proteins; D012260:Ribonucleases; D012694:Serine; D013053:Spectrophotometry; D013237:Stereoisomerism; D013997:Time Factors
D000438:Alcohols; D000814:Aniline Compounds; D007202:Indicators and Reagents; D011506:Proteins; D010504:Periodic Acid; D012694:Serine; D012260:Ribonucleases