title: The enzymatic preparation of isolated intact parenchymal cells from rat liver.
abstract: Suspensions of isolated parenchymal cells were prepared from rat liver by incubation with collagenase and hyaluronidase followed by mechanical treatment. Utilization of 0.15% collagenase together with 0.15% hyaluronidase yielded adequate numbers of cells for experimental purposes. As shown by light and electron microscopy, approximately 75% of the isolated cells retain their structural integrity. The cell suspensions are capable of maintaining endogenous respiration in the presence of 1% albumin for periods of time up to 8 hr. These cell preparations consist almost entirely of parenchymal cells and offer a unique tissue preparation for the study of hepatic metabolism.
mesh_terms: D000818:Animals; D002467:Cell Nucleus; D004721:Endoplasmic Reticulum; D006652:Histological Techniques; D006821:Hyaluronoglucosaminidase; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008297:Male; D003012:Microbial Collagenase; D008854:Microscopy, Electron; D008930:Mitochondria, Liver; D010101:Oxygen Consumption; D051381:Rats
chemical_list: D006821:Hyaluronoglucosaminidase; D003012:Microbial Collagenase