Separation of the bovine colostrum M-1 glycoprotein into two components.
1. Two glycoproteins were isolated from the M-1 acid glycoprotein fraction of bovine colostrum. 2. The lighter glycoprotein had a molecular weight of 7200, contained about 28.4% of carbohydrate, and had an absorption maximum at 275nm. The heavier glycoprotein had a molecular weight of 12000, contained 39.0% of carbohydrate, and had no absorption maxima in the 240-300nm. range of the spectrum. 3. The carbohydrate moiety of both glycoproteins was removable from the polypeptide moiety under the conditions of the beta-elimination reaction. 4. Periodate oxidation experiments showed that sialic acid was linked to galactose in both proteins.
D000818:Animals; D001896:Boron Compounds; D002241:Carbohydrates / Q000032:analysis; D002417:Cattle; D002850:Chromatography, Gel; D003126:Colostrum / Q000032:analysis*; D004586:Electrophoresis; D005260:Female; D006023:Glycoproteins / Q000032:analysis / Q000302:isolation & purification*; D008970:Molecular Weight; D010504:Periodic Acid; D013053:Spectrophotometry; D014461:Ultracentrifugation
D001896:Boron Compounds; D002241:Carbohydrates; D006023:Glycoproteins; D010504:Periodic Acid