title: Effect of poliovirus on deoxyribonucleic acid synthesis in HeLa cells.
abstract: Ackermann, W. W. (University of Michigan, Ann Arbor), D. C. Cox, H. Kurtz, C. D. Powers, and S. J. Davies. Effect of poliovirus on deoxyribonucleic acid synthesis in HeLa cells. J. Bacteriol. 91:1943-1952. 1966.-Both poliovirus and arginine stimulated deoxyribonucleic acid (DNA) synthesis in cultures of HeLa cells which were preconditioned by incubation in a medium deficient in arginine. However, the number of cells producing DNA was unaffected. DNA synthesis in such preconditioned cells was 10 to 20% of the maximal value obtained with a full complement of amino acids. Inhibition of DNA synthesis was produced in these cultures either by increasing the multiplicity of exposure above 40 plaque-forming units of virus per cell or by increasing the concentration of the deficient amino acid at the time of virus addition. Inhibition of DNA synthesis resulted from a reduction in the fraction of cells producing DNA. The concentration of arginine required for viral inhibition of DNA synthesis is greater than that for viral multiplication.
mesh_terms: D001120:Arginine / Q000494:pharmacology; D001345:Autoradiography; D002172:Canavanine / Q000494:pharmacology; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis*; D006146:Guanidines / Q000494:pharmacology; D006367:HeLa Cells; D006639:Histidine / Q000494:pharmacology; D017955:Poliovirus; D014316:Tritium
chemical_list: D006146:Guanidines; D014316:Tritium; D002172:Canavanine; D006639:Histidine; D004247:DNA; D001120:Arginine