Site of host restriction of simian virus 40 mutants in an established African green monkey kidney cell line.
A previously described large-plaque morphology mutant of SV40 (SV-L) in primary African green monkey kidney (AGMK) cells has been shown to be restricted in the established AGMK line CV-1. The site of restriction is an early step in infection, involving the virus particle. A block in penetration or uncoating has been tentatively proposed. Restriction is observed in a number of other established lines, including monkey, human, and mouse. Independent large-plaque mutants were isolated in AGMK and their properties were compared with SV-L (temperature restriction, host restriction, and virion antigen). They can be separated into two classes. Both are restricted on CV-1, though to a lesser degree than SV-L. Further-more, both are coat mutants as determined by immunological analysis of their virions, suggesting a relationship between coat mutants associated with large-plaque morphology in AGMK and host restriction in established cell lines. Temperature restriction is seen with one but not the other of the mutants.
D000327:Adsorption; D000818:Animals; D000998:Antiviral Agents; D002460:Cell Line; D003168:Complement Fixation Tests; D046508:Culture Techniques; D004279:DNA, Viral / Q000633:toxicity; D000882:Haplorhini; D007668:Kidney; D009154:Mutation; D010759:Phosphorus Isotopes; D013539:Simian virus 40 / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity*; D013696:Temperature
D000998:Antiviral Agents; D004279:DNA, Viral; D010759:Phosphorus Isotopes