Brush border particulates of renal tissue.
Particulates containing a large part of the alkaline phosphatase activity of renal tissue were separated from homogenates and from ribosomal preparations by zonal centrifugation. The particles had a high content of phospholipid and cholesterol that was not removed by treatment with I percent deoxycholate. Enzymatic activities concentrated with the particles were the alkaline phosphatase, a peptidase resistant to proteolysis, glucose-6-phosphatase, inorganic pyrophos-phatase, and adenosine triphosphatase. The particles accumulated leucine with no stimulation from soluble factors and with inhibition by other amino acids; the accumulation was stimulated by adenosine triphosphate and was not inhibited by puromycin. The particles appear to be derived from the membranes of the brush borders of tubular cells.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D002247:Carbon Isotopes; D002501:Centrifugation, Zonal; D005952:Glucose-6-Phosphatase / Q000378:metabolism*; D007684:Kidney Tubules / Q000166:cytology / Q000201:enzymology*; D007930:Leucine / Q000378:metabolism; D008566:Membranes / Q000201:enzymology*; D008854:Microscopy, Electron; D010447:Peptide Hydrolases / Q000378:metabolism*; D011691:Puromycin / Q000494:pharmacology; D011755:Pyrophosphatases / Q000378:metabolism*; D051381:Rats; D012270:Ribosomes / Q000201:enzymology*; D013501:Surface-Active Agents
D002247:Carbon Isotopes; D013501:Surface-Active Agents; D011691:Puromycin; D000255:Adenosine Triphosphate; D000469:Alkaline Phosphatase; D005952:Glucose-6-Phosphatase; D010447:Peptide Hydrolases; D000251:Adenosine Triphosphatases; D011755:Pyrophosphatases; D007930:Leucine