Characterization of rapidly labelled rat liver ribonucleic acid showing high affinity for columns of methylated albumin on kieselguhr.
Most of the rapidly labelled RNA from rat liver submitted to column chromatography on methylated albumin on kieselguhr remains tightly bound to the column and can only be recovered by elution with m-ammonia. The tightly bound RNA is composed mainly of DNA-like RNA. The binding capacity is dependent not only on base composition but also on molecular size: the heavier RNA molecules show a greater affinity to the column than do the lower-molecular-weight components. Rapidly labelled mouse liver and Saccharomyces cerevisiae RNA show similar behaviour to rat liver RNA on columns of methylated albumin on kieselguhr.
D000418:Albumins; D000641:Ammonia; D000818:Animals; D002499:Centrifugation, Density Gradient; D002845:Chromatography; D008099:Liver / Q000032:analysis*; D008297:Male; D008745:Methylation; D051379:Mice; D008970:Molecular Weight; D009963:Orotic Acid; D012313:RNA / Q000032:analysis*; D051381:Rats; D012440:Saccharomyces; D012822:Silicon Dioxide; D014316:Tritium
D000418:Albumins; D014316:Tritium; D009963:Orotic Acid; D012313:RNA; D012822:Silicon Dioxide; D000641:Ammonia