Isolation and partial characterization of a major inositol-containing lipid in baker's yeast, mannosyl-diinositol, diphosphoryl-ceramide.
A polar lipid containing about 20 per cent of the lipid soluble inositol has been purified from cells of Saccharomyces cerevisiae. This compound labeled with (3)H-inositol, (32)P(i), or (14)C glucose was isolated from aqueous pyridine extracts by means of paper chromatography. Unlabeled compound was obtained by a differential solubility scheme. Analytical and degradation studies suggest that this compound can be formulated as a mannosyl-diinositol, diphosphoryl-ceramide. It can be converted by treatment with base or acid into a mannosylmonoinositol phosphoryl-ceramide, a composition which relates it to the basic structure of the phytoglycolipids.
D002247:Carbon Isotopes; D002554:Cerebrosides / Q000032:analysis* / Q000302:isolation & purification; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D005947:Glucose / Q000378:metabolism; D006851:Hydrochloric Acid; D006878:Hydroxides; D007294:Inositol / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D010716:Phosphatidylinositols / Q000032:analysis* / Q000302:isolation & purification; D010759:Phosphorus Isotopes; D012440:Saccharomyces / Q000032:analysis*; D014316:Tritium
D002247:Carbon Isotopes; D002554:Cerebrosides; D006878:Hydroxides; D010710:Phosphates; D010716:Phosphatidylinositols; D010759:Phosphorus Isotopes; D014316:Tritium; D007294:Inositol; D005947:Glucose; D006851:Hydrochloric Acid