Bacteriophage-induced functions in Escherichia coli K (lambda) infected with rII mutants of bacteriophage T4.
Rutberg, Blanka (Karolinska Institutet, Stockholm, Sweden), and Lars Rutberg. Bacteriophage-induced functions in Escherichia coli K(lambda) infected with rII mutants of bacteriophage T4. J. Bacteriol. 91:76-80. 1966.-When Escherichia coli K(lambda) was infected with rII mutants of phage T4, deoxycytidine triphosphatase, one of the phage-induced early enzymes, was produced at initially the same rate as in r(+)-infected cells. Deoxyribonuclease activity was one-third to one-half of that of r(+)-infected cells. This lower deoxyribonuclease activity was observed also in other hosts or when infection was made with rI or rIII mutants. Presence of chloramphenicol did not allow a continued synthesis of phage deoxyribonucleic acid in rII-infected K(lambda). No phage lysozyme was detected nor was any antiphage serum-blocking antigen found in rII-infected K(lambda). It is suggested that the rII gene is of significance for the expression of phage-induced late functions in the host K(lambda).
D000941:Antigens; D002701:Chloramphenicol / Q000494:pharmacology; D003090:Coliphages / Q000201:enzymology / Q000276:immunology; D004279:DNA, Viral / Q000096:biosynthesis; D003851:Deoxyribonucleases / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology*; D066298:In Vitro Techniques; D009113:Muramidase; D009154:Mutation; D010744:Phosphoric Monoester Hydrolases / Q000096:biosynthesis*
D000941:Antigens; D004279:DNA, Viral; D002701:Chloramphenicol; D003851:Deoxyribonucleases; D010744:Phosphoric Monoester Hydrolases; D009113:Muramidase