title: Antigenic relationship of the gram-negative organism causing canine abortion to smooth and rough brucellae.
abstract: The use of whole-cell antigens in agglutination and agglutinin-absorption tests showed that the organism causing abortion in dogs is similar to rough Brucella abortus, B. melitensis, and B. ovis, but different from smooth Brucella cultures. Water-soluble antigens obtained by ultrasonic treatment and examined by immuno-electrophoresis and gel diffusion show extensive cross-reactions within the genus Brucella, but little or no cross-reaction with similar antigens from other gram-negative genera in the family Brucellaceae. The dog organism showed near identity with rough and smooth Brucella cultures on the basis of immuno-gel diffusion tests with water-soluble antigens, but it lacked the lipopolysaccharide-endotoxin associated with the agglutinogen of smooth brucellae. These findings support the proposal of Carmichael and Bruner for the designation of a new species, "Brucella canis."
mesh_terms: D000034:Abortion, Veterinary / Q000382:microbiology*; D000372:Agglutination Tests; D000818:Animals; D000941:Antigens / Q000032:analysis*; D001886:Bordetella pertussis / Q000276:immunology; D002002:Brucella / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D005260:Female; D006193:Haemophilus influenzae / Q000276:immunology; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D010325:Pasteurella / Q000276:immunology; D011247:Pregnancy
chemical_list: D000941:Antigens