Proteins of vesicular stomatitis virus. I. Polyacrylamide gel analysis of viral antigens.
Infection of L cells with vesicular stomatitis virus results in the release, into the cell-free fluid, of four antigenic components separable by rate zonal centrifugation on sucrose gradients. The largest antigens are the infectious (B) particle and a shorter noninfectious, autointerfering (T) particle. The two small antigens are characterized by sedimentation coefficients of approximately 20S and 6S. Treatment of purified B or T particles with sodium deoxycholate results in the release from the particle of a nucleoprotein core which can be purified on sucrose gradient and which has a sedimentation coefficient characteristic of the virus from which it arose. Utilizing purified antigens labeled with (14)C-amino acids during growth, we examined the protein constituents of each antigen by acrylamide-gel electrophoresis. The proteins of B and T particles are identical, each containing one minor (virus protein 1) and three major (virus proteins 2, 3, and 4) proteins, numbered in order of increasing mobility. Virus protein 3 originates from the nucleoprotein core, whereas proteins 2 and 4 come from the coat. The origin of virus protein 1 is not known. The 20S antigen contains a single protein equivalent to virus protein 3, whereas the 6S antigen shows a single protein which is similar to, but probably distinct from, virus protein 2.
D000596:Amino Acids / Q000378:metabolism; D000941:Antigens / Q000032:analysis* / Q000302:isolation & purification; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D003168:Complement Fixation Tests; D003513:Cycloheximide / Q000494:pharmacology; D003588:Cytopathogenic Effect, Viral; D004589:Electrophoresis, Disc; D007739:L Cells; D014721:Vesicular stomatitis Indiana virus / Q000032:analysis* / Q000187:drug effects; D014764:Viral Proteins / Q000032:analysis* / Q000302:isolation & purification
D000596:Amino Acids; D000941:Antigens; D002247:Carbon Isotopes; D014764:Viral Proteins; D003513:Cycloheximide