Characterization of vitamin K from bovine liver.
Concentrated fractions of vitamin K from bovine liver were purified by thin-layer chromatography and fractions were analyzed by UV spectroscopy and mass spectrometry. The chromatographic behavior of the purified vitamins was compared with that of known compounds on thin layers of silica gel, either untreated or impregnated with silver nitrate or paraffin. The principal forms of vitamin K recovered from bovine liver were highly lipophilic. Two fractions were obtained which collectively gave identifiable mass spectra of menaquinone-10, menaquinone-11, and menaquinone-12.
D000818:Animals; D002417:Cattle; D002855:Chromatography, Thin Layer; D008099:Liver / Q000032:analysis*; D012822:Silicon Dioxide; D013053:Spectrophotometry; D013057:Spectrum Analysis; D014466:Ultraviolet Rays; D014812:Vitamin K / Q000032:analysis*
D014812:Vitamin K; D012822:Silicon Dioxide