Synthesis of ribonucleic acid in cells infected with LSc poliovirus at elevated temperatures.
The synthesis of viral ribonucleic acid (RNA) was detected within 2 hr after infection with LSc poliovirus at 35 C. This RNA eluted as a single peak with 0.9 m NaCl on methylated albumin celite columns, was sensitive to ribonuclease, precipitated in the presence of 2 m LiCl, and had an S(20) value at 34 +/- 2 in linear sucrose gradients. When cells were infected at 39 to 40 C, there was also early synthesis of RNA. However, 2 hr after infection this synthesis was drastically inhibited. The absence of net RNA synthesis at 39 to 40 C during the late stages of infection was not caused by rapid degradation of newly formed RNA, since the RNA produced between 1 and 2 hr at 39 to 40 C was still present 3.5 hr after infection. There was a 3 log(10) inhibition in the production of infectious virus when p-fluorophenylalanine was present in the medium at a concentration of 25 mug/ml. This concentration of analogue had little effect upon the production of viral polymerase and viral RNA. Virus grown in the presence of analogue at a concentration of 10 mug/ml exhibited increased heat sensitivity compared to control virus. However, viral polymerase exhibited no change in sensitivity to heat or manganese when cells were exposed to 25 mug of p-fluorophenylalanine per ml during infection. p-Fluorophenylalanine had a relatively selective effect on viral capsid protein but did not reverse the inhibition of synthesis of viral RNA at 39 to 40 C.
D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002845:Chromatography; D003609:Dactinomycin / Q000494:pharmacology; D006367:HeLa Cells / Q000187:drug effects; D008345:Manganese / Q000494:pharmacology; D010649:Phenylalanine / Q000494:pharmacology; D017955:Poliovirus / Q000187:drug effects; D012313:RNA / Q000096:biosynthesis; D012316:RNA Nucleotidyltransferases / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis* / Q000302:isolation & purification; D013696:Temperature; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects
D012367:RNA, Viral; D014316:Tritium; D003609:Dactinomycin; D008345:Manganese; D010649:Phenylalanine; D012313:RNA; D012316:RNA Nucleotidyltransferases; D014529:Uridine