Continuous cultivation of virus in cell suspensions by use of the lysostat.
Theoretical considerations illustrate the feasibility and characteristics of continuous virus cultivation in cell suspensions Physical dimensions of the virus fermentor (lysostat) in relation to optimal yields can be predetermined. Poliovirus type 1 was grown on Hela S-3-1 (Saltzman) cells in a single-stage, percolator-type lysostat, with a yield of 421 TCID(50) per cell. Adenovirus type 14 was grown on KB (Eagle) cells in a two-stage lysostat with a yield of 116 TCID(50) per cell.
D046508:Culture Techniques; D006367:HeLa Cells; D017955:Poliovirus / Q000254:growth & development; D014776:Virus Cultivation / Q000295:instrumentation*; D014780:Viruses / Q000302:isolation & purification*