Conversion of glucose-1-phosphate to 3-keto-glucose-1-phosphate by cells of Agrobacterium tumefaciens.
Incubation of resting cells of Agrobacterium tumefaciens with glucose-1-phosphate resulted in the accumulation of a new sugar phosphate in the suspending medium. Approximately 80% of the glucose-1-phosphate consumed was converted to the new compound, which was identified as alpha-d-ribo-hexopyranosyl-3-ulose-1-phosphate (3-ketoglucose-1-phosphate). Both utilization of glucose-1-phosphate and accumulation of 3-ketoglucose-1-phosphate were inhibited by 2,4-dinitrophenol, polymyxin, and d-glucose, which are inhibitors of the glucoside transport system of this bacterium but are not inhibitors of d-glucoside-3-dehydrogenase, which is the 3-ketoglucose-1-phosphate-forming enzyme. Consequently, it was concluded that glucose-1-phosphate penetrates into intracellular space by means of an active transport system. The glucose-1-phosphate is converted to 3-ketoglucose-1-phosphate by d-glucoside-3-dehydrogenase, and the 3-ketoglucose-1-phosphate formed reaches the extracellular space by passing through the surface layer of the bacterium.
D001693:Biological Transport, Active; D002854:Chromatography, Paper; D004140:Dinitrophenols / Q000494:pharmacology; D004586:Electrophoresis; D005947:Glucose / Q000494:pharmacology; D006600:Hexosephosphates / Q000032:analysis / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D011113:Polymyxins / Q000494:pharmacology; D012231:Rhizobium / Q000201:enzymology / Q000378:metabolism*; D013053:Spectrophotometry
D004140:Dinitrophenols; D006600:Hexosephosphates; D011113:Polymyxins; D010088:Oxidoreductases; D005947:Glucose