D-glucaric acid and galactaric acid catabolism by Agrobacterium tumefaciens.
Cell-free extract (crude extract) of Agrobacterium tumefaciens grown on d-glucuronate or d-glucarate converts d-glucarate and galactarate to a mixture of 2-keto-3-deoxy- and 4-deoxy-5-keto-d-glucarate. These compounds are then converted by partially purified crude extract to an intermediate tentatively identified as 2,5-diketoadipate. The same enzyme preparation further decarboxylates this intermediate to alpha-ketoglutarate semialdehyde, which is subsequently oxidized in a nicotinamide adenine dinucleotide-dependent reaction to alpha-ketoglutaric acid. Since A. tumefaciens converts d-glucuronic acid to d-glucarate, a pathway from d-glucuronate to alpha-ketoglutarate in A. tumefaciens was determined.
D000272:Adipates / Q000378:metabolism*; D002247:Carbon Isotopes; D002498:Centrifugation; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D004586:Electrophoresis; D005965:Glucuronates / Q000378:metabolism; D006836:Hydro-Lyases / Q000378:metabolism; D007656:Ketoglutaric Acids / Q000096:biosynthesis; D009243:NAD / Q000494:pharmacology; D010209:Paper; D000644:Quaternary Ammonium Compounds; D012231:Rhizobium / Q000201:enzymology / Q000378:metabolism*; D013053:Spectrophotometry; D013431:Sulfates; D014466:Ultraviolet Rays
D000272:Adipates; D002247:Carbon Isotopes; D005965:Glucuronates; D007656:Ketoglutaric Acids; D000644:Quaternary Ammonium Compounds; D013431:Sulfates; D009243:NAD; D006836:Hydro-Lyases