Nature of the pressor substance in rabbit placenta.
1. The renin-like substance isolated from the placenta of the rabbit produces a prolonged increase of blood pressure in the nephrectomized rat. If incubated with renin substrate from ox serum, it forms a pressor substance that elevates blood pressure in exactly the same way as does angiotensin. 2. This angiotensin-like principle was concentrated by means of ion-exchange chromatography and compared with Val-5-angiotensins I and II in two paper-chromatography systems and in paper electrophoresis. 3. In all the three methods the unknown principle behaved like a mixture of the two reference compounds. 4. It is concluded that incubation of the renin-like substance of placental origin with substrate from ox serum gave a mixture of Val-5-angiotensins I and II. This is evidence that the enzyme isolated from the placenta is either closely related to, or identical with, renin.
D000804:Angiotensin II; D000818:Animals; D001681:Biological Assay; D001794:Blood Pressure / Q000187:drug effects*; D002852:Chromatography, Ion Exchange; D002854:Chromatography, Paper; D004586:Electrophoresis; D005260:Female; D066298:In Vitro Techniques; D009392:Nephrectomy; D010920:Placenta / Q000032:analysis* / Q000201:enzymology*; D011247:Pregnancy; D011270:Pregnancy, Animal; D011817:Rabbits; D051381:Rats; D012083:Renin / Q000097:blood*
D000804:Angiotensin II; D012083:Renin