Nicotinamide-adenine dinucleotide-specific isocitrate dehydrogenase from pea mitochondria. Purification and properties.
1. A method of stabilizing the enzyme by using glycerol is described. 2. A purification procedure is presented giving a higher purification than previously described. 3. Data showing substrate activation and activation by citrate are presented. 4. Kinetic constants for NAD(+), NADH and certain bivalent metal ions are given. 5. Pronounced inhibitory buffer effects are described. 6. A brief comparison between the NAD-specific isocitrate dehydrogenase from peas and that from other sources is made.
D000227:Adenine Nucleotides; D002482:Cellulose; D002852:Chromatography, Ion Exchange; D002951:Citrates; D004355:Drug Stability; D005990:Glycerol; D006863:Hydrogen-Ion Concentration; D007477:Ions; D007521:Isocitrate Dehydrogenase / Q000037:antagonists & inhibitors; D007700:Kinetics; D008722:Methods; D008928:Mitochondria / Q000201:enzymology*; D009243:NAD; D010944:Plants / Q000201:enzymology*; D013053:Spectrophotometry; D013268:Stimulation, Chemical
D000227:Adenine Nucleotides; D002951:Citrates; D007477:Ions; D009243:NAD; D002482:Cellulose; D007521:Isocitrate Dehydrogenase; D005990:Glycerol