Production of interferon in serum-free human leukocyte suspensions.
The recovery of interferon from Sendai-infected suspensions of purified human leukocytes is dependent on the serum concentration in the incubation medium. Very little interferon is obtained from serum-free suspensions. The data reported demonstrate that the critical macromolecular, age-independent, and species-unspecific serum principle can be omitted from the suspensions if the medium is supplemented with a combination of crystalline serum albumin and high concentrations of any one of five studied dipolar ionic buffers [N,N-bis(2-hydroxyethyl) glycine (Bicine), N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES), 2-(N-morpholino)ethanesulfonic acid (MES), N-tris(hydroxymethyl)methyl-2-amino-ethanesulfonic acid (TES), and N-tris(hydroxymethyl)methylglycine (Tricine)]. The optimal combination [TES (1.0%, w/v) and bovine serum albumin (0.8%, w/v)] allows the production of potent preparations of serum-free human interferon.
D000818:Animals; D002021:Buffers; D002417:Cattle; D002642:Chick Embryo; D003470:Culture Media; D005998:Glycine; D006168:Guinea Pigs; D006801:Humans; D007106:Immune Sera; D007372:Interferons / Q000096:biosynthesis*; D007962:Leukocytes / Q000378:metabolism*; D051379:Mice; D010222:Parainfluenza Virus 1, Human; D011817:Rabbits; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D012756:Sheep; D013451:Sulfonic Acids
D002021:Buffers; D003470:Culture Media; D007106:Immune Sera; D012709:Serum Albumin; D013451:Sulfonic Acids; D012710:Serum Albumin, Bovine; D007372:Interferons; D005998:Glycine