Altered specificity of transfer-ribonucleic acid nucleotidyltransferase in the presence of manganese.
1. s-RNA nucleotidyltransferase incorporated CMP into phosphodiesterase-treated s-RNA more rapidly in the presence of Mg(2+) (10mm) than in the presence of Mn(2+) (2mm). UMP was incorporated more rapidly in the presence of Mn(2+), and at high ionic strength the incorporation of CMP was also more rapid in the presence of Mn(2+). 2. The capacity of phosphodiesterase-treated s-RNA for CMP, UMP and AMP was increased in the presence of Mn(2+). Terminal sequences of more than one UMP or AMP residue were synthesized, but these atypical reactions were inhibited when CTP was added. CMP was incorporated rapidly to form -pCpC terminal sequences and then more slowly as longer chains were formed, but very little CMP was incorporated into s-RNA-pCpCpA. 3. CMP was incorporated into phosphodiesterase-treated 5s RNA and ribosomal RNA to form short chains of polyC attached to the primer RNA. This reaction was inhibited by the presence of s-RNA. 4. A small Mn(2+)-dependent incorporation of CMP was also primed by poly(A).(U) and poly(C).(I).
D000227:Adenine Nucleotides / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002247:Carbon Isotopes; D003597:Cytosine Nucleotides / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D011119:Polynucleotides / Q000378:metabolism*; D012316:RNA Nucleotidyltransferases / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D051381:Rats; D013057:Spectrum Analysis; D014500:Uracil Nucleotides / Q000378:metabolism
D000227:Adenine Nucleotides; D002247:Carbon Isotopes; D003597:Cytosine Nucleotides; D011119:Polynucleotides; D014500:Uracil Nucleotides; D008345:Manganese; D012343:RNA, Transfer; D012316:RNA Nucleotidyltransferases; D010744:Phosphoric Monoester Hydrolases; D008274:Magnesium