Comparative intermediary metabolism of vegetative cells and microcysts of Myxococcus xanthus.
Crude extracts of both vegetative cells and glycerol-induced microcysts of Myxococcus xanthus contained the following enzyme activities: phosphofructokinase, phosphoglucoisomerase, fructose-1,6-diphosphatase, fructosediphosphate aldolase, glyceraldehyde-3-phosphate dehydrogenase, phosphopyruvate carboxylase, citrate synthase, isocitrate dehydrogenase, alpha-ketoglutarate dehydrogenase, succinate dehydrogenase, malate dehydrogenase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, phosphoglucomutase, and uridine diphosphate glucose pyrophosphorylase. With the exception of isocitrate dehydrogenase, which was present at a fivefold higher concentration in microcysts, all activities in extracts from both types of cells were essentially equal. Hexokinase and pyruvate kinase could not be detected in extracts from either type of cell. Microcysts metabolized acetate at a lower rate than did vegetative cells. Most of this decrease was reflected in a substantial decrease in ability of microcysts to oxidize acetate to CO(2). In addition, microcysts and vegetative cells showed a different distribution of (14)C-label from incorporated acetate.
D000085:Acetates / Q000378:metabolism; D001419:Bacteria / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D002247:Carbon Isotopes; D002262:Carboxy-Lyases / Q000378:metabolism; D002474:Cell-Free System; D002952:Citric Acid Cycle; D006597:Fructose-Bisphosphatase / Q000378:metabolism; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005990:Glycerol / Q000494:pharmacology; D006593:Hexokinase / Q000378:metabolism; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D007656:Ketoglutaric Acids; D008190:Lyases / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D009713:Nucleotidyltransferases / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D010101:Oxygen Consumption; D010732:Phosphofructokinase-1 / Q000378:metabolism; D010733:Phosphoglucomutase / Q000378:metabolism; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism; D013170:Spores / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism
D000085:Acetates; D002247:Carbon Isotopes; D007656:Ketoglutaric Acids; D010088:Oxidoreductases; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D013385:Succinate Dehydrogenase; D006593:Hexokinase; D010732:Phosphofructokinase-1; D011770:Pyruvate Kinase; D009713:Nucleotidyltransferases; D006597:Fructose-Bisphosphatase; D008190:Lyases; D002262:Carboxy-Lyases; D005634:Fructose-Bisphosphate Aldolase; D005956:Glucose-6-Phosphate Isomerase; D010733:Phosphoglucomutase; D005947:Glucose; D005990:Glycerol