An automatic apparatus for the study of enzyme kinetics.
A continuous-flow apparatus is described for automatically plotting substrate saturation curves, and is suitable for use with a variety of enzymes. A linear concentration gradient of the variable substrate is combined with a fixed proportion of the other substrates and the enzyme, and after passing through a reaction coil the product concentrations are measured spectrophotometrically. Use of a 4cm. flow cell and modified spectrophotometer permits accurate measurement of NADH concentration in the region of 0.1mum. Precise control over reaction times and substrate concentration is achieved by using power-driven syringes with an integral mixer. Specimen results are given for yeast alcohol dehydrogenase.
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D001322:Autoanalysis; D003201:Computers; D004798:Enzymes / Q000378:metabolism*; D007700:Kinetics; D009243:NAD / Q000032:analysis; D012440:Saccharomyces / Q000201:enzymology; D013053:Spectrophotometry / Q000295:instrumentation*; D013594:Syringes
D004798:Enzymes; D009243:NAD; D000429:Alcohol Oxidoreductases