Investigations on metabolites of vitamin D in rat bile. Separation and partial identification of a major metabolite.
1. Young rats with cannulated bile ducts were given 0.34mg. of [1alpha-(3)H]cholecalciferol or 0.54mg. of [(14)C]ergocalciferol by intravenous infusion. Of the radioactivity in the dose of [1alpha-(3)H]cholecalciferol 31% was recovered in bile within 24hr. 2. The metabolites in bile were separated by gradient-elution column chromatography on silicic acid into five components, all more polar than cholecalciferol or 25-hydroxycholecalciferol. [(14)C]Ergocalciferol gave a similar pattern of metabolites in bile. 3. The three most polar metabolites were shown to be ionic. The major component has been identified as a glucuronide conjugate, which was not identical with synthetic cholecalciferyl glucuronide.
D000818:Animals; D001646:Bile / Q000032:analysis*; D002247:Carbon Isotopes; D002762:Cholecalciferol / Q000378:metabolism; D002845:Chromatography; D004872:Ergocalciferols / Q000378:metabolism; D005260:Female; D005965:Glucuronates / Q000032:analysis; D005966:Glucuronidase; D008297:Male; D051381:Rats; D012822:Silicon Dioxide; D014316:Tritium; D014807:Vitamin D / Q000378:metabolism*
D002247:Carbon Isotopes; D004872:Ergocalciferols; D005965:Glucuronates; D014316:Tritium; D014807:Vitamin D; D002762:Cholecalciferol; D012822:Silicon Dioxide; D005966:Glucuronidase