title: 2,4-diaminovaleric acid: an intermediate in the anaerobic oxidation of ornithine by Clostridium sticklandii.
abstract: The oxidation of ornithine in the presence of proline by crude extracts of Clostridium sticklandii cells was stimulated by nicotinamide adenine dinucleotide, coenzyme A, alpha-ketoglutarate, dimethylbenzimidazolyl cobamide (DBC) coenzyme, MgCl(2), and adenosine diphosphate. Deletion of various cofactors resulted in the accumulation of a new dibasic amino acid which was identified as 2,4-diaminovaleric acid. Both the oxidation of ornithine to alanine and acetate and the conversion of ornithine to 2,4-diaminovaleric acid were stimulated by addition of DBC coenzyme, and both were inhibited by intrinsic factor, an inhibitor of cobamide coenzyme-dependent reactions. This inhibition was reversed by addition of DBC coenzyme. However, the oxidation of 2,4-diaminovaleric acid was insensitive to added intrinsic factor. The data indicate that 2,4-diaminovaleric acid represents the first intermediate in the oxidation of ornithine by C. sticklandii.
mesh_terms: D000085:Acetates / Q000378:metabolism; D000227:Adenine Nucleotides / Q000494:pharmacology; D000409:Alanine / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D002247:Carbon Isotopes; D002712:Chlorides / Q000494:pharmacology; D002845:Chromatography; D003013:Clostridium / Q000187:drug effects / Q000378:metabolism*; D003067:Coenzymes / Q000494:pharmacology; D004586:Electrophoresis; D007437:Intrinsic Factor / Q000494:pharmacology; D007656:Ketoglutaric Acids / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D009243:NAD / Q000494:pharmacology; D009952:Ornithine / Q000378:metabolism*; D010084:Oxidation-Reduction; D011392:Proline / Q000378:metabolism; D014631:Valerates / Q000096:biosynthesis*; D014805:Vitamin B 12 / Q000494:pharmacology
chemical_list: D000085:Acetates; D000227:Adenine Nucleotides; D000596:Amino Acids; D002247:Carbon Isotopes; D002712:Chlorides; D003067:Coenzymes; D007656:Ketoglutaric Acids; D014631:Valerates; D009243:NAD; D007437:Intrinsic Factor; D011392:Proline; D009952:Ornithine; D008274:Magnesium; D000409:Alanine; D014805:Vitamin B 12