Role of interferon in the propagation of MM virus in L cells.
MM virus propagated in mouse brain replicates to low titers in L cells without production of cytopathic effect (CPE). After growing the virus in BHK-21 cells, however, the virus replicates to high titers in L cells with complete CPE. It was found that suspensions of MM virus propagated in L cells directly from the mouse brain contained much more interferon than did suspensions of virus which had first been grown in BHK-21 cells. Mouse brain suspensions of the virus were also found to contain high interferon titers. Treatment of L cells with actinomycin D before infection with mouse brain-grown virus resulted in full virus replication with CPE. BHK-21 cell-grown virus diluted in L cell interferon behaved like mouse brain-grown virus in L cells. It is concluded that the presence of interferon in the inoculum is largely responsible for the suppression of MM virus replication in L cells.
D000818:Animals; D001921:Brain; D002460:Cell Line; D006224:Cricetinae; D003588:Cytopathogenic Effect, Viral; D003609:Dactinomycin / Q000494:pharmacology; D004686:Encephalomyocarditis virus / Q000254:growth & development*; D007372:Interferons / Q000494:pharmacology*; D007668:Kidney; D007739:L Cells; D051379:Mice; D014357:Trypsin / Q000494:pharmacology; D014776:Virus Cultivation; D014779:Virus Replication / Q000187:drug effects*
D003609:Dactinomycin; D007372:Interferons; D014357:Trypsin