Studies on glycogen synthesis in pigeon liver homogenates. Glycogen synthesis from glucose monophosphates and uridine diphosphate glucose.
Comparative time-course studies of glycogen synthesis from glucose 6-phosphate, glucose 1-phosphate and UDP-glucose show that glucose 1-phosphate forms glycogen at an initial rate faster than that obtained with glucose 6-phosphate and UDP-glucose. After 5min. the rates from glucose monophosphates are considerably slower. 2,4-Dinitrophenol decreases glycogen synthesis from both glucose monophosphates, whereas arsenate and EDTA increase glycogen synthesis from glucose 1-phosphate and inhibit the reaction from glucose 6-phosphate, galactose and galactose 1-phosphate. Mitochondria-free pigeon liver cytoplasmic fraction forms less glycogen from glucose monophosphates than does the whole homogenate. 2-Deoxyglucose 6-phosphate inhibits glycogen synthesis from glucose monophosphates. Glycogen formation from UDP-glucose is relatively unaffected by dinitrophenol, by arsenate, by EDTA, by 2-deoxyglucose 6-phosphate and by the removal of mitochondria from the whole homogenate.
D000818:Animals; D001152:Arsenicals / Q000494:pharmacology; D002247:Carbon Isotopes; D010856:Columbidae; D003593:Cytoplasm / Q000378:metabolism; D003864:Depression, Chemical; D004140:Dinitrophenols / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D005952:Glucose-6-Phosphatase / Q000378:metabolism*; D007700:Kinetics; D008112:Liver Glycogen / Q000096:biosynthesis*; D008930:Mitochondria, Liver / Q000378:metabolism; D051381:Rats; D013268:Stimulation, Chemical; D014500:Uracil Nucleotides / Q000378:metabolism*
D001152:Arsenicals; D002247:Carbon Isotopes; D004140:Dinitrophenols; D008112:Liver Glycogen; D014500:Uracil Nucleotides; D004492:Edetic Acid; D005952:Glucose-6-Phosphatase; D005947:Glucose