Isoleucine and valine metabolism of Escherichia coli. XV. Biochemical properties of mutants resistant to thiaisoleucine.
Thiaisoleucine-resistant mutants of Escherichia coli strain K-12 which exhibited reduced isoleucyl soluble ribonucleic acid synthetase activity were isolated. Resistance was apparently achieved by the selection of a synthetase with a 10-fold decrease in apparent affinity for thiaisoleucine. This mutation also resulted in a 2.5-fold decrease in apparent affinity for the natural substrate, l-isoleucine, and less activity than found in wild type. The mutants grew more slowly than wild type and were derepressed for three of the five enzymes in the pathways to isoleucine and valine.
D002247:Carbon Isotopes; D002845:Chromatography; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D006836:Hydro-Lyases / Q000378:metabolism; D007532:Isoleucine / Q000378:metabolism* / Q000494:pharmacology; D008025:Ligases / Q000378:metabolism; D009154:Mutation; D010504:Periodic Acid / Q000494:pharmacology; D012343:RNA, Transfer / Q000378:metabolism; D000637:Transaminases / Q000378:metabolism; D014633:Valine / Q000378:metabolism*
D002247:Carbon Isotopes; D007532:Isoleucine; D010504:Periodic Acid; D012343:RNA, Transfer; D000637:Transaminases; D006836:Hydro-Lyases; D008025:Ligases; D014633:Valine