Rapid identification of viruses by indirect immunofluorescence: standardization and use of antiserum pool to nine respiratory viruses.
The indirect fluorescent antibody test employing treated and standardized antisera and conjugated antiglobulin has been used successfully, in conjunction with a technique for growing and staining virus cell systems in situ on microscope slides, in the identification of nine respiratory viruses. By using pooled antisera in a single test, the presence or absence of these viruses was determined in 18 to 45 hr after inoculation of slide microtissue culture.
D000256:Adenoviridae / Q000302:isolation & purification; D000257:Adenoviridae Infections / Q000175:diagnosis*; D000818:Animals; D002642:Chick Embryo; D046508:Culture Techniques / Q000295:instrumentation; D005189:False Positive Reactions; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D006561:Herpes Simplex / Q000175:diagnosis*; D006801:Humans; D007106:Immune Sera; D007251:Influenza, Human / Q000175:diagnosis*; D008722:Methods; D008856:Microscopy, Fluorescence; D009107:Mumps / Q000175:diagnosis*; D009109:Mumps virus / Q000302:isolation & purification; D009975:Orthomyxoviridae / Q000302:isolation & purification; D009976:Orthomyxoviridae Infections / Q000175:diagnosis*; D012136:Respiratory Syncytial Viruses / Q000302:isolation & purification*; D018108:Respirovirus / Q000302:isolation & purification; D018139:Simplexvirus / Q000302:isolation & purification
D007106:Immune Sera