Dehydrogenase patterns in the study of Bacteroidaceae.
Enzyme patterns were obtained by starch-gel electrophoresis of cell-free extracts from organisms in the family Bacteroidaceae. Esterases, phosphatases, and lactic and succinic dehydrogenases were detected but offered no possibility for classification purposes. Alpha-glycerophosphate dehydrogenase and 6-phosphogluconate dehydrogenase were not detected. Zymograms of malic, glutamic, and glucose-6-phosphate dehydrogenases separated Bacteroides species from Sphaerophorus and Fusobacterium species. Malic dehydrogenase and glucose-6-phosphate dehydrogenase were found in Bacteroides but not Sphaerophorus and Fusobacterium. These dehydrogenase zymograms placed three gram-negative, nonsporeforming anaerobic rod isolates with the Bacteroides species. A close correlation was found between the classification of Bacteroidaceae by zymogram analysis and a numerical taxonomy scheme previously published from this laboratory.
D001419:Bacteria / Q000145:classification / Q000201:enzymology*; D001439:Bacteroides / Q000145:classification / Q000201:enzymology; D004586:Electrophoresis; D004950:Esterases / Q000032:analysis; D005673:Fusobacterium / Q000145:classification / Q000201:enzymology; D005782:Gels; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D005969:Glutamate Dehydrogenase / Q000032:analysis; D005993:Glycerolphosphate Dehydrogenase / Q000032:analysis; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008291:Malate Dehydrogenase / Q000032:analysis; D010088:Oxidoreductases / Q000032:analysis*; D010734:Phosphogluconate Dehydrogenase / Q000032:analysis; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis; D013213:Starch; D013385:Succinate Dehydrogenase / Q000032:analysis
D005782:Gels; D013213:Starch; D010088:Oxidoreductases; D005993:Glycerolphosphate Dehydrogenase; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D013385:Succinate Dehydrogenase; D005969:Glutamate Dehydrogenase; D004950:Esterases; D010744:Phosphoric Monoester Hydrolases