Investigations on vitamin D esters synthesized rats. Detection and identification.
1. Vitamin D-deficient rachitic rats were given [1-(3)H]cholecalciferol by gastric intubation. After 24hr., diethyl ether extracts of liver and kidney contained 5-11% and 4.5-20% respectively of total vitamin D apparently esterified with long-chain fatty acids. 2. A two-dimensional thin-layer chromatographic technique was devised that completely separated seven synthetic vitamin D esters according to the chain length and number of double bonds in the fatty acid component. When the ;vitamin D ester' fraction from liver or kidney was co-chromatographed with the standard esters, radioactivity appeared mainly in vitamin D palmitate, stearate, oleate and linoleate regions. The proportion of radioactivity in the saturated fatty acid esters was higher in kidney than in liver. 3. The same percentage of tissue vitamin D in the esterified form was found at each of two dosages of vitamin D. 4. The possible specificity of a vitamin D esterification mechanism is discussed.
D000818:Animals; D002762:Cholecalciferol / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D004032:Diet; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D012279:Rickets / Q000378:metabolism*; D012822:Silicon Dioxide; D014316:Tritium; D014807:Vitamin D / Q000096:biosynthesis*
D005227:Fatty Acids; D014316:Tritium; D014807:Vitamin D; D002762:Cholecalciferol; D012822:Silicon Dioxide