Avirulent Clostridium perfringens strains obtained by euflavine treatment.
Clostridium perfringens was incubated in the presence of euflavine (EU); the resistant mutants which were thus isolated had highly reduced capacity to release alpha-toxin. This fact was confirmed by lecithinase determinations and by immunoelectrophoresis. Injected into guinea pigs and into 6- to 7-day-old chicks, these mutants were completely avirulent. A study of their properties indicated biochemical differences between wild types and mutants.
D000166:Acridines / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D050260:Carbohydrate Metabolism; D002364:Caseins / Q000378:metabolism; D002645:Chickens; D003015:Clostridium Infections; D003016:Clostridium perfringens / Q000187:drug effects* / Q000201:enzymology / Q000472:pathogenicity; D005260:Female; D006168:Guinea Pigs; D006461:Hemolysis; D006863:Hydrogen-Ion Concentration; D007122:Immunoelectrophoresis; D008297:Male; D051379:Mice; D009154:Mutation / Q000187:drug effects; D010740:Phospholipases / Q000096:biosynthesis / Q000378:metabolism; D014118:Toxins, Biological / Q000096:biosynthesis; D014774:Virulence
D000166:Acridines; D002364:Caseins; D014118:Toxins, Biological; D010740:Phospholipases