Selective inhibition of bacterial enzymes by free fatty acids.
Octanoic acid inhibits, in vitro, the bacterial enzymes glucose-6-phosphate dehydrogenase, phosphofructokinase, pyruvate kinase, fumarase, lactate dehydrogenase, and the malic enzyme of Arthrobacter crystallopoietes. The free fatty acid appears to act as an inhibitor of lipogenesis, although it does not affect the rate of gluconeogenesis. To demonstrate that this inhibition may be of physiological significance in vivo, those enzymes not involved in lipogenesis, such as fructose-1, 6-diphosphatase, phosphoglucomutase, phosphohexoisomerase, aconitase, nicotinamide adenine dinucleotide phosphate (NADP) isocitrate dehydrogenase, NADP glutamate dehydrogenase, malate dehydrogenase, and isocitrate lyase, were assayed and found not to be inhibited by the free fatty acid.
D001173:Arthrobacter / Q000187:drug effects* / Q000201:enzymology; D002474:Cell-Free System; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology*; D006597:Fructose-Bisphosphatase / Q000037:antagonists & inhibitors; D005956:Glucose-6-Phosphate Isomerase / Q000037:antagonists & inhibitors; D005954:Glucosephosphate Dehydrogenase / Q000037:antagonists & inhibitors; D005969:Glutamate Dehydrogenase / Q000037:antagonists & inhibitors; D006836:Hydro-Lyases / Q000037:antagonists & inhibitors; D007521:Isocitrate Dehydrogenase / Q000037:antagonists & inhibitors; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors; D008190:Lyases / Q000037:antagonists & inhibitors; D008291:Malate Dehydrogenase / Q000037:antagonists & inhibitors; D010732:Phosphofructokinase-1 / Q000037:antagonists & inhibitors; D010733:Phosphoglucomutase / Q000037:antagonists & inhibitors; D011770:Pyruvate Kinase / Q000037:antagonists & inhibitors
D005230:Fatty Acids, Nonesterified; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D005969:Glutamate Dehydrogenase; D010732:Phosphofructokinase-1; D011770:Pyruvate Kinase; D006597:Fructose-Bisphosphatase; D008190:Lyases; D006836:Hydro-Lyases; D005956:Glucose-6-Phosphate Isomerase; D010733:Phosphoglucomutase