Carcinogens 3,4-benzpyrene and 3-methylcholanthrene: induction of mitochondrial oxidative enzymes.
Sections of liver from rats injected with 3,4-benzpyrene and 3-methylcholanthrene, when incubated in mediums specific for the histochemical demonstration of mitochondrial oxidative enzymes, show greater activity of several of these enzymes than do sections from control rats. This observation was confirmed by comparison of the staining of mitochondria isolated from the control and from "induced" rats. The fact that an inhibitor of protein synthesis, actinomycin D, effectively diminished the stimulation provided evidence that the stimulation of activity is due to an increase in enzyme synthesis, generally called induction.
D000818:Animals; D001580:Benzopyrenes / Q000494:pharmacology*; D003609:Dactinomycin / Q000494:pharmacology; D008058:Dihydrolipoamide Dehydrogenase / Q000096:biosynthesis*; D003576:Electron Transport Complex IV / Q000096:biosynthesis*; D004790:Enzyme Induction; D005969:Glutamate Dehydrogenase / Q000096:biosynthesis*; D005993:Glycerolphosphate Dehydrogenase / Q000096:biosynthesis*; D006884:Hydroxybutyrate Dehydrogenase / Q000096:biosynthesis*; D007770:L-Lactate Dehydrogenase / Q000096:biosynthesis*; D008291:Malate Dehydrogenase / Q000096:biosynthesis*; D008748:Methylcholanthrene / Q000494:pharmacology*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology*; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats; D013268:Stimulation, Chemical; D013385:Succinate Dehydrogenase / Q000096:biosynthesis*; D013778:Tetrazolium Salts; D014284:Triiodothyronine / Q000494:pharmacology*
D001580:Benzopyrenes; D013778:Tetrazolium Salts; D014284:Triiodothyronine; D003609:Dactinomycin; D008748:Methylcholanthrene; D005993:Glycerolphosphate Dehydrogenase; D007770:L-Lactate Dehydrogenase; D006884:Hydroxybutyrate Dehydrogenase; D008291:Malate Dehydrogenase; D013385:Succinate Dehydrogenase; D005969:Glutamate Dehydrogenase; D008058:Dihydrolipoamide Dehydrogenase; D003576:Electron Transport Complex IV; D010634:Phenobarbital