Osteolytic sterol in human breast cancer.
Eleven of twelve human breast cancers contained a lipid which increased urinary (45)Ca and (40)Ca excretion of (45)Ca-labeled, parathyroidectomized rats receiving a low Ca diet. The lipid has mobility on thin-layer chromatography and gas-liquid chromatography close to, but not identical with, that of 7-dehydrocholesterol. Authentic 7-dehydrocholesterol has osteolytic activity similar to that of the extracted sterol. Fluorescence and Lieberman-Burchard reactions of the extracted sterol are similar to those of 7-dehydrocholesterol. The lipid was found by thin-layer chromatography in the extracts which had osteolytic activity. Neither the lipid nor osteolytic activity was found in extracts of tissue from two normal human breasts.
D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002127:Calcium Isotopes; D002762:Cholecalciferol; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer / Q000209:etiology; D003649:Decalcification, Pathologic / Q000187:drug effects; D005260:Female; D005453:Fluorescence; D006801:Humans; D066298:In Vitro Techniques; D011874:Radiometry; D051381:Rats / Q000494:pharmacology; D013261:Sterols / Q000472:pathogenicity*; D014556:Urine; D014807:Vitamin D
D002127:Calcium Isotopes; D013261:Sterols; D014807:Vitamin D; D002762:Cholecalciferol; D002118:Calcium