The phosphatidylinositol kinase of rat brain.
1. The presence of a phosphatidylinositol kinase in homogenates of adult rat brain was shown by using labelled ATP or labelled phosphatidylinositol. 2. The kinase was activated by Mg(2+) or Mn(2+) and inhibited by Ca(2+), Cu(2+), K(+), Na(+) and F(-). 3. The detergents sodium deoxycholate, Cutscum and Triton X-100 markedly stimulated the reaction; sodium taurocholate, Tween-20 and cetyltrimethyl-ammonium bromide were less effective. 4. The activity of the enzyme was dependent on SH groups. 5. The subcellular distribution of the kinase in brain resembled that of Na(+)-plus-K(+)-stimulated adenosine triphosphatase and 5'-nucleotidase.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000166:cytology / Q000201:enzymology*; D002118:Calcium; D003300:Copper; D005459:Fluorides; D066298:In Vitro Techniques; D008274:Magnesium; D008345:Manganese; D009708:Nucleotidases / Q000378:metabolism; D010716:Phosphatidylinositols; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism*; D011188:Potassium; D051381:Rats; D012964:Sodium; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013501:Surface-Active Agents
D010716:Phosphatidylinositols; D013501:Surface-Active Agents; D008345:Manganese; D003300:Copper; D012964:Sodium; D010734:Phosphogluconate Dehydrogenase; D013385:Succinate Dehydrogenase; D010770:Phosphotransferases; D009708:Nucleotidases; D000251:Adenosine Triphosphatases; D008274:Magnesium; D005459:Fluorides; D011188:Potassium; D002118:Calcium