title: Metabolism of mannitol by Coccidioides immitis.
abstract: Lones, George W. (National Institute of Allergy and Infectious Diseases, U.S. Public Health Service, Bethesda, Md.), and Carl Peacock. Metabolism of mannitol by Coccidioides immitis. J. Bacteriol. 87:1114-1117. 1964.-Strain M-11 of Coccidioides immitis was found to utilize mannitol for growth in the mycelial form but not in the spherule form. Cell-free extracts of both forms, grown on glucose, were capable of reducing nicotinamide adenine dinucleotide with mannitol-1-PO(4) but not with mannitol. The extracts accomplished a rapid oxidation of reduced nicotinamide adenine dinucleotide by fructose-6-PO(4), the expected product of mannitol-1-PO(4) oxidation. Fructose was inactive. Paper electrophoresis and chromatography with several solvent systems demonstrated a substance in extracts of both mycelium and spherules having a migration consistent with that of mannitol.
mesh_terms: D003045:Coccidioides / Q000254:growth & development / Q000378:metabolism*; D005993:Glycerolphosphate Dehydrogenase / Q000096:biosynthesis; D008353:Mannitol / Q000032:analysis / Q000378:metabolism*; D009243:NAD / Q000378:metabolism
chemical_list: D009243:NAD; D008353:Mannitol; D005993:Glycerolphosphate Dehydrogenase