A stimulation by cyclic 3',5'-adenosine monophosphate of amino acid activation and polymerization in reticulocyte hemolysates.
With reticulocyte supernatant, cyclic 3',5'-adenosine monophosphate at concentrations of 10(-3) to 10(-2)M causes stimulation of aminoacyl-tRNA synthetases for some, e.g., valine and leucine, but not all, amino acids; it is highest at nonsaturating concentrations of ATP. Similar concentrations of cyclic 3',5'-adenosine monophosphate are found to stimulate phenylalanine polymerization from phenylalanyl transfer ribonucleic acid on polyuridylic acid-charged reticulocyte ribosomes. The degree of stimulation is highest at low GTP concentrations. It is abolished by addition of phosphoenolpyruvate + pyruvate kinase, which stimulate similarly or more effectively at low GTP levels. Under the conditions of these experiments, cyclic 3',5'-adenosine monophosphate did not appreciably inhibit GTP hydrolysis.
D000227:Adenine Nucleotides / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism*; D002247:Carbon Isotopes; D000242:Cyclic AMP / Q000494:pharmacology; D006150:Guanine Nucleotides / Q000378:metabolism; D008025:Ligases / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D010452:Peptide Biosynthesis; D010649:Phenylalanine / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism; D012156:Reticulocytes / Q000378:metabolism*; D013268:Stimulation, Chemical; D014316:Tritium; D014633:Valine / Q000378:metabolism
D000227:Adenine Nucleotides; D000596:Amino Acids; D002247:Carbon Isotopes; D006150:Guanine Nucleotides; D011773:Pyruvates; D014316:Tritium; D010649:Phenylalanine; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D011770:Pyruvate Kinase; D008025:Ligases; D014633:Valine; D008239:Lysine