Fluorescent-antibody studies with antisera against heated and unheated poliovirus type 1.
Fluorescent-antibody (FA) reagents were prepared from sera of guinea pigs immunized with either native infectious poliovirus type 1 or poliovirus type 1 which had been heated at 56 C for 30 min. Conjugates made from sera of animals immunized with heated virus gave higher direct FA staining titers on air-dried, acetone-fixed, infected cells than conjugates made from sera of animals immunized with native infectious virus. Evidence was obtained that complement-fixing antibody reactive with heated antigen was responsible for the FA staining. Two conjugates prepared from sera of guinea pigs immunized with heated poliovirus type 1 were successfully used to identify 21 type 1 viruses isolated from a group of 44 stool suspensions studied as unknowns. These conjugates did not stain any of 23 heterologous enteroviruses present in the remainder of the stools and gave minimal non-specific staining.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D003168:Complement Fixation Tests; D005243:Feces / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D006358:Hot Temperature; D007106:Immune Sera; D009500:Neutralization Tests; D017955:Poliovirus / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification
D000906:Antibodies; D007106:Immune Sera