title: Decarboxylation of 2-keto fatty acids by brain.
abstract: An enzyme system consisting of washed acetone powder of pig brain, adenosine triphosphate, nicotinamide adenine dinucleotide, magnesium, fumaric acid, and ascorbic acid was found to catalyze the oxidative decarboxylation of 2-ketostearic acid. The products were carbon dioxide and heptadecanoic acid. Washing the enzyme powder with ethylene-diaminetetraacetate and other chelating agents destroyed the activity. Further properties of the enzyme system are described. It is believed that this enzyme system accounts in part for the 1-carbon degradation route for brain fatty acids.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D001921:Brain / Q000201:enzymology* / Q000378:metabolism*; D002262:Carboxy-Lyases / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D005650:Fumarates / Q000378:metabolism; D066298:In Vitro Techniques; D007501:Iron / Q000378:metabolism; D007651:Keto Acids / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D013229:Stearic Acids / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D013552:Swine
chemical_list: D005227:Fatty Acids; D005650:Fumarates; D007651:Keto Acids; D013229:Stearic Acids; D009243:NAD; D000255:Adenosine Triphosphate; D007501:Iron; D002262:Carboxy-Lyases; D008274:Magnesium; D001205:Ascorbic Acid