title: Transport of reduced nicotinamide-adenine dinucleotide into mitochondria of rat white adipose tissue.
abstract: Mitochondria from rat white adipose tissue were prepared, exhibiting good respiratory control and P/O ratios. They would not oxidize NADH unless NNN'N'-tetramethyl-p-phenylenediamine was added as a carrier of reducing equivalents. These mitochondria were found to oxidize neither l-glycerol 3-phosphate nor l-glutamate plus l-malate at significant rates. The activity of aspartate aminotransferase in these mitochondria was found to be low compared with that found in rat liver mitochondria. As a consequence of this, the adipose-tissue mitochondria exhibited very low rates of cytoplasmic NADH oxidation in a reconstituted Borst (1962) cycle compared with liver mitochondria.
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000814:Aniline Compounds; D000818:Animals; D001219:Aspartate Aminotransferases / Q000032:analysis; D001692:Biological Transport; D005971:Glutamates / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism; D066298:In Vitro Techniques; D008293:Malates / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D009243:NAD / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats
chemical_list: D000814:Aniline Compounds; D005971:Glutamates; D005994:Glycerophosphates; D008293:Malates; D009243:NAD; D001219:Aspartate Aminotransferases