The synthesis of polydeoxyribonucleotide by cell-free extracts of embryonic mouse tissue.
The conditions under which soluble extracts prepared from mouse embryos incorporate [(3)H]thymidine 5'-triphosphate into polydeoxyribonucleotide have been studied. In common with similar preparations from other mammalian tissues, mouse-embryo DNA nucleotidyltransferase requires the four complementary deoxyribonucleoside 5'-triphosphates, primer DNA and a bivalent cation for activity. Unlike other mammalian DNA nucleotidyltransferases, the rate and extent of the incorporation of [(3)H]thymidine 5'-triphosphate is much greater with Mn(2+) than with Mg(2+) and, with either Mg(2+) or Mn(2+), maximum activity occurs at pH6.4. The difference between Mg(2+) and Mn(2+) varies markedly with pH, reaching a maximum of six- to eight-fold at pH6.4.
D000818:Animals; D004622:Embryo, Mammalian / Q000201:enzymology / Q000378:metabolism*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D051379:Mice; D009691:Nucleic Acid Denaturation; D009711:Nucleotides / Q000378:metabolism*; D009713:Nucleotidyltransferases / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases; D011119:Polynucleotides / Q000096:biosynthesis*; D014020:Tissue Extracts; D014316:Tritium
D009711:Nucleotides; D011119:Polynucleotides; D014020:Tissue Extracts; D014316:Tritium; D008345:Manganese; D009713:Nucleotidyltransferases; D010744:Phosphoric Monoester Hydrolases; D008274:Magnesium