Detergent-solubilized RNA polymerase from cells infected with foot-and-mouth disease virus.
The foot-and-mouth disease virus RNA polymerase complex was dissociated from cellular membranes with deoxycholate in the presence of dextran sulfate. The soluble polymerase complex was active in the cell-free synthesis of virus-specific RNA; solubilization of the complex permitted direct analysis of the cell-free reaction mixtures without recourse to RNA extraction. A major RNA-containing component found early during cell-free incubation ranged from approximately 140 to 300S. The final major products of the cell-free system were 37S virus RNA, 20S ribonuclease-resistant RNA, and a 50S component containing RNA.
D005537:Aphthovirus / Q000201:enzymology*; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D003902:Detergents / Q000494:pharmacology*; D012316:RNA Nucleotidyltransferases / Q000032:analysis* / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis*; D012995:Solubility; D013395:Sucrose; D014316:Tritium; D014500:Uracil Nucleotides / Q000378:metabolism; D014776:Virus Cultivation
D003902:Detergents; D012367:RNA, Viral; D014500:Uracil Nucleotides; D014316:Tritium; D013395:Sucrose; D012316:RNA Nucleotidyltransferases