Interaction of the carbohydrate-binding protein concanavalin A with normal and transformed cells.
It has been shown that the carbohydrate-binding protein concanavalin A (ConA) can agglutinate leukemic cells and cells transformed by polyoma virus, simian virus 40, chemical carcinogens, and X-irradiation. This protein did not agglutinate normal cells under the same conditions. The agglutination was reversed by competition with alpha-methyl-D-glucopyranoside (alpha-MG), a carbohydrate that strongly binds to ConA, but not by the carbohydrates alpha-methyl-L-fucopyranoside or N-acetylglucosamine, with no binding or weak binding to ConA. Destruction of the alpha-MG binding sites of the native protein by removal of bivalent metal ions abolished the agglutination produced by the native protein. The treatment of cells with trypsin resulted in the agglutination of normal cells by ConA and a decrease of agglutinability of transformed cells. When nonagglutinating untransformed 3T3 cells were infected with simian virus 40 and normal rat cells were infected with polyoma virus, the infected cells became agglutinable several days after virus infection. The percentage of cells agglutinated, about 50 per cent, was much higher than the percentage of cells hereditarily transformed. The results indicate that the surface membrane of transformed cells contains sites that interact with the alpha-MG binding sites of ConA, that such sites can be found on the surface membrane of normal cells after treatment with trypsin, and that the change in the surface structure from normal to transformed occurs in cells that are abortively transformed.
D000371:Agglutination / Q000187:drug effects; D000818:Animals; D001665:Binding Sites; D002241:Carbohydrates; D002471:Cell Transformation, Neoplastic; D006224:Cricetinae; D046508:Culture Techniques; D006801:Humans; D007942:Leukemia, Experimental; D010936:Plant Extracts; D011120:Polyomavirus; D011485:Protein Binding; D051381:Rats; D014357:Trypsin
D002241:Carbohydrates; D010936:Plant Extracts; D014357:Trypsin