A new method for the quantitative determination of monosaccharides, amino sugars and N-acetylneuraminic acid and of 6-deoxyhexose (fucose) in the presence of other sugars.
1. Monosaccharides, amino sugars and N-acetylneuraminic acid were determined by using an original colorimetric assay procedure, based on the detection of formaldehyde released after periodate oxidation. A range of these compounds was investigated by this method and they were all found to obey Beer's law within the concentration range 0-0.6mumole/ml. 2. A simple method for the determination of 6-deoxyhexose concentration in the presence of other monosaccharides is also described. 3. The optimum pH for the release of formaldehyde from sugars by periodate oxidation was 7.0-7.5. 4. The methods described have considerable advantages over existing assay systems and their particlar value in automatic colorimetry, where the use of concentrated acids is undesirable, is discussed.
D000606:Amino Sugars / Q000032:analysis*; D001322:Autoanalysis; D003124:Colorimetry; D005557:Formaldehyde; D005643:Fucose / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D009005:Monosaccharides / Q000032:analysis*; D009438:Neuraminic Acids / Q000032:analysis*; D010504:Periodic Acid
D000606:Amino Sugars; D009005:Monosaccharides; D009438:Neuraminic Acids; D010504:Periodic Acid; D005557:Formaldehyde; D005643:Fucose