Murine hepatitis virus: effect on liver RNA.
After infection of mice with hepatitis virus MHV3, the RNA in the liver undergoes changes. The fraction extracted with phenol at 0 degrees C does not alter. However, the fraction extracted with hot phenol at elevated pH (60 degrees C, pH 8.3) shows a 16S peak on sucrose-density-gradient centrifugation. This fraction shows actinomycin D-resistant incorporation of C(14)-orotic acid in infected but not in control livers-possible evidence of the RNA nature of MHV3.
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D002247:Carbon Isotopes; D003609:Dactinomycin / Q000494:pharmacology; D006506:Hepatitis A / Q000378:metabolism*; D006520:Hepatitis, Animal / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008099:Liver / Q000032:analysis / Q000378:metabolism*; D051379:Mice; D006517:Murine hepatitis virus; D009963:Orotic Acid / Q000378:metabolism; D012313:RNA / Q000032:analysis / Q000096:biosynthesis*
D002247:Carbon Isotopes; D003609:Dactinomycin; D009963:Orotic Acid; D012313:RNA; D007770:L-Lactate Dehydrogenase; D000410:Alanine Transaminase