title: Action of streptolysin S, the group D hemolysin, and phospholipase C on whole cells and spheroplasts.
abstract: Davie, Joseph M. (Indiana University, Bloomington), and Thomas D. Brock. Action of streptolysin S, the group D hemolysin, and phospholipase C on whole cells and spheroplasts. J. Bacteriol. 91:595-600. 1966.-The effect of streptolysin S, the group D hemolysin, and phospholipase C (the alpha toxin of Clostridium perfringens) on whole cells and spheroplasts or protoplasts of three strains of streptococci and Micrococcus lysodeikticus was tested. Viability, C(14)-glycine uptake, and lysis were measured. The group D hemolysin and phospholipase C were active against whole bacteria; streptolysin S was not. All three substances were active on spheroplasts. A partially resistant mutant derived from a strain sensitive to the group D hemolysin was also partially resistant to streptolysin S and phospholipase C. Antimycin A protected spheroplasts from streptolysin S but not from the group D hemolysin.
mesh_terms: D000968:Antimycin A / Q000494:pharmacology; D001433:Bacteriolysis; D002247:Carbon Isotopes; D003016:Clostridium perfringens; D013293:Enterococcus faecalis / Q000378:metabolism*; D005998:Glycine / Q000378:metabolism; D006460:Hemolysin Proteins / Q000494:pharmacology*; D066298:In Vitro Techniques; D008837:Micrococcus / Q000378:metabolism*; D010740:Phospholipases / Q000494:pharmacology*; D013301:Streptolysins / Q000494:pharmacology*; D014118:Toxins, Biological
chemical_list: D002247:Carbon Isotopes; D006460:Hemolysin Proteins; D013301:Streptolysins; D014118:Toxins, Biological; D000968:Antimycin A; D010740:Phospholipases; D005998:Glycine