Internal standards in the estimation of acetyl-CoA in liver extracts.
The necessity for adding an internal standard to liver extracts during fluorimetric estimation of acetyl-CoA by the malic dehydrogenase-citrate synthase reaction is demonstrated. Addition of acetyl-CoA completely compensates for the inhibitory action of some tissue components. Values for hepatic acetyl-CoA in fed and fasted rats are given.
D000818:Animals; D001835:Body Weight; D055598:Chemical Phenomena; D002621:Chemistry; D003065:Coenzyme A / Q000032:analysis*; D005453:Fluorescence; D008099:Liver / Q000737:chemistry*; D008190:Lyases; D008293:Malates; D009243:NAD; D009929:Organ Size; D051381:Rats; D014020:Tissue Extracts / Q000032:analysis
D008293:Malates; D014020:Tissue Extracts; D009243:NAD; D008190:Lyases; D003065:Coenzyme A