Heat-labile serum factor required for immunofluorescence of polyoma tumor antigens.
The immunofluorescent demonstration of polyoma tumor antigens in tumor cells requires a heatlabile serum component. With unheated hamster tumor serum, specific fluorescence was observed in polyoma-transformed hamster, mouse, and rat tumor cells. Heated serum usually gave little or no reactivity; the activity of such heated serum could be restored simply by the addition of fresh normal unheated hamster serum.
D000818:Animals; D000941:Antigens; D003165:Complement System Proteins; D006224:Cricetinae; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D000882:Haplorhini; D006801:Humans; D051379:Mice; D011120:Polyomavirus; D011200:Poultry; D051381:Rats
D000941:Antigens; D003165:Complement System Proteins