Cyclic 3',5'-adenosine monophosphate phosphodiesterase produced by the slime mold Dictyostelium discoideum.
An enzyme that hydrolyzes cyclic ',5'-adenosine monophosphate to 5'-adenosine monophosphate was found in the culture medium of the cellular slime mold Dictyostelium discoidum. The enzymatic activity shows a pH optimum of 7.5, and magnesium is required for maximum activity. The enzyme is not inhibited by caffeine. It has a Michaelis-Menten constant of 2 x 10(-3)M and its molecular weight is around 300,000.
D000227:Adenine Nucleotides / Q000378:metabolism*; D002122:Calcium Chloride; D002712:Chlorides; D002850:Chromatography, Gel; D000242:Cyclic AMP / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008274:Magnesium; D008345:Manganese; D008970:Molecular Weight; D009235:Myxomycetes / Q000201:enzymology*; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis / Q000096:biosynthesis*; D012710:Serum Albumin, Bovine / Q000032:analysis
D000227:Adenine Nucleotides; D002712:Chlorides; D012710:Serum Albumin, Bovine; D008345:Manganese; D000242:Cyclic AMP; D010744:Phosphoric Monoester Hydrolases; D008274:Magnesium; D002122:Calcium Chloride