A rapid simple method for the assay of renin in rabbit plasma.
1. EDTA (10mm), 2,3-dimercaptopropan-1-ol (10mm) and chlorhexidine gluconate (0.005%, w/v) cause complete inactivation of plasma enzymes that degrade angiotensin I, but have no effect on the reaction of renin with its substrate. The reagents were termed the selective inhibitors. 2. Thus it is possible to measure renin in plasma by its ability to catalyse the release of angiotensin I. 3. Sterile plasma, treated with the selective inhibitors, is incubated with renin substrate (500-1000ng. of angiotensin content/ml.) at pH6 at 42 degrees for 6hr. 4. Under these conditions the reaction obeys first-order kinetics. Renin activity is calculated in terms of the percentage release of the angiotensin content/hr. 5. As described, the assay is sufficiently sensitive to measure renin in the plasma of all normal rabbits. By extending the length of the incubation, much lower activities can be measured.
D000438:Alcohols; D000804:Angiotensin II; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D003377:Countercurrent Distribution; D004492:Edetic Acid; D005942:Gluconates; D006863:Hydrogen-Ion Concentration; D007610:Kallikreins / Q000037:antagonists & inhibitors; D007700:Kinetics; D008722:Methods; D011817:Rabbits; D012083:Renin / Q000097:blood*; D013696:Temperature; D000092502:Renin Inhibitors
D000438:Alcohols; D000804:Angiotensin II; D004492:Edetic Acid; D005942:Gluconates; D007610:Kallikreins; D012083:Renin; D000092502:Renin Inhibitors