title: Components required for the formation of CH-4 from methylcobalamin by extracts of Methanobacillus omelianskii.
abstract: Wood, J. M. (University of Illinois, Urbana), and R. S. Wolfe. Components required for the formation of CH(4) from methylcobalamin by extracts of Methanobacillus omelianskii. J. Bacteriol. 92:696-700. 1966.-Optimal conditions for the formation of CH(4) from methylcobalamin (methyl-cobalt-5,6-dimethylbenzimidazolylcobamide) by partially purified extracts of Methanobacillus omelianskii (Methanobacterium omelianskii) were achieved by the addition of two protein fractions, adenosine triphosphate, Mg(++), and a reduced flavin adenine dinucleotide-generating system. Adenosine diphosphate and adenosine monophosphate play an important role in the regulation of C(1) transfer in this reaction. The possible position of adenosine triphosphate in B(12)-dependent methyl transfer is discussed.
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism; D001407:Bacillus / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D008274:Magnesium / Q000378:metabolism; D008697:Methane / Q000096:biosynthesis*; D009243:NAD / Q000378:metabolism; D009711:Nucleotides / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D014805:Vitamin B 12 / Q000378:metabolism*
chemical_list: D000227:Adenine Nucleotides; D009711:Nucleotides; D011506:Proteins; D009243:NAD; D008274:Magnesium; D008697:Methane; D014805:Vitamin B 12