Lipid composition of chloroplasts isolated by aqueous and nonaqueous techniques.
Chloroplasts isolated from tobacco leaves in 0.5 M sucrose solution (the 1000 g pellet) contained 83% of the total cellular monogalactosyl diglyceride, 88% of the digalactosyl diglyceride, 76% of the sulfolipid, and 74% of the phosphatidyl glycerol. Phosphatidyl inositol was concentrated in the 15,000 g pellet. Phosphatidyl choline and phosphatidyl ethanolamine were concentrated in the 15,000 g supernatant fraction. Chloroplasts isolated from tobacco leaves by a nonaqueous technique in hexane-carbon tetrachloride show a glycerolipid composition similar to that found in chloroplasts isolated in the aqueous system, even though some lipid, particularly monogalactosyl diglyceride, is extracted by the organic solvent during the process.
D000473:Alkanes; D002251:Carbon Tetrachloride; D002734:Chlorophyll / Q000032:analysis; D002736:Chloroplasts / Q000032:analysis* / Q000166:cytology; D005612:Freeze Drying; D005989:Glycerides / Q000032:analysis; D006027:Glycosides / Q000032:analysis; D008055:Lipids / Q000032:analysis*; D008854:Microscopy, Electron; D010713:Phosphatidylcholines / Q000032:analysis; D010714:Phosphatidylethanolamines / Q000032:analysis; D010716:Phosphatidylinositols / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*; D010940:Plant Proteins / Q000032:analysis; D010947:Plants, Toxic; D013455:Sulfur; D014026:Nicotiana / Q000032:analysis* / Q000166:cytology; D014867:Water
D000473:Alkanes; D005989:Glycerides; D006027:Glycosides; D008055:Lipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010716:Phosphatidylinositols; D010743:Phospholipids; D010940:Plant Proteins; D014867:Water; D002734:Chlorophyll; D013455:Sulfur; D002251:Carbon Tetrachloride