Phosphorus determination in phosphoglycerides from thin-layer chromatograms.
Phosphoglyceride phosphorus is readily determined directly on silica gel removed from thin-layer chromatograms, without elution, by a nondigestive procedure with a sulfuric-periodic acid reagent. The method is specific for hydrolyzable phosphoglyceride containing two acid-hydrolyzable groups. Cariolipin reacts only partially, while sphingomyelin, diether lecithin, and phosphonate cephalin fail completely to react.
D002855:Chromatography, Thin Layer; D005994:Glycerophosphates / Q000032:analysis*; D007202:Indicators and Reagents; D008244:Lysophosphatidylcholines / Q000032:analysis; D008722:Methods; D010504:Periodic Acid; D010713:Phosphatidylcholines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D010758:Phosphorus / Q000032:analysis*; D013109:Sphingomyelins / Q000032:analysis; D013464:Sulfuric Acids
D005994:Glycerophosphates; D007202:Indicators and Reagents; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D010743:Phospholipids; D013109:Sphingomyelins; D013464:Sulfuric Acids; D010504:Periodic Acid; D010758:Phosphorus