title: Ileal loop fluid accumulation and production of diarrhea in rabbits by cell-free products of Clostridium perfringens.
abstract: The ability of cell extracts and culture filtrates of various strains of C. perfringens to produce ileal loop fluid accumulation and overt diarrhea in rabbits was tested. Good correlation was obtained in the ability of whole cells and a toxic factor (present in cell extracts and concentrated culture filtrates) to produce both fluid accumulation in ileal loops and diarrhea when injected into the normal ileum of the rabbit. The toxic factor was present in cell-free preparations when cells were grown in a sporulation medium, but not when they were grown in an asporogenic medium. The factor was shown to be heat labile, nondialyzable, and was inactivated by Pronase but not by trypsin, lipase, or amylase. Loss of activity occurred at pH 1.0, 3.0, 5.0, and 12.0.
mesh_terms: D000681:Amylases / Q000494:pharmacology; D000818:Animals; D001826:Body Fluids / Q000378:metabolism*; D002474:Cell-Free System; D003015:Clostridium Infections; D003016:Clostridium perfringens / Q000032:analysis*; D003470:Culture Media; D003967:Diarrhea / Q000209:etiology*; D004195:Disease Models, Animal; D005260:Female; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007082:Ileum / Q000378:metabolism*; D008049:Lipase / Q000494:pharmacology; D008297:Male; D010447:Peptide Hydrolases / Q000494:pharmacology; D011817:Rabbits; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology
chemical_list: D003470:Culture Media; D008049:Lipase; D000681:Amylases; D010447:Peptide Hydrolases; D014357:Trypsin