title: Application of cell fractionation techniques in the study of cells infected with polyoma virus and Newcastle disease virus.
abstract: Fisher, Harold W. (University of Rhode Island, Kingston), Hidemi Matsumiya, and Masanobu Azuma. Application of cell fractionation techniques in the study of cells infected with polyoma virus and Newcastle disease virus. J. Bacteriol. 91:1645-1651. 1966.-Techniques which permitted rigorous separation of nuclei and cytoplasm were applied to the study of the formation of Newcastle disease virus (NDV) in an established line of Chinese hamster cells and of polyoma virus (PYV) in mouse embryo fibroblasts. The results obtained for hemagglutinin and plaque-forming titers during virus growth were in agreement with those obtained by others, using different techniques. These indicated that NDV matures in the cytoplasm, and PYV in the nucleus, of host cells.
mesh_terms: D000818:Animals; D002467:Cell Nucleus; D002623:Chemistry Techniques, Analytical; D006224:Cricetinae; D003593:Cytoplasm; D005347:Fibroblasts; D005954:Glucosephosphate Dehydrogenase; D006385:Hemagglutination Inhibition Tests; D066298:In Vitro Techniques; D008858:Microscopy, Phase-Contrast; D009522:Newcastle disease virus; D011120:Polyomavirus; D014776:Virus Cultivation
chemical_list: D005954:Glucosephosphate Dehydrogenase