title: Biosynthesis and composition of histones in Novikoff hepatoma nuclei and nucleoli.
abstract: Histones were prepared from isolated nuclei and nucleoli of the Novikoff ascitic hepatoma at several time points after the injection of L-lysine uniformly labeled with C(14) into tumor-bearing rats. Amino acid analysis and starch-gel electrophoresis failed to reveal any differences between the nuclear and nucleolar histones, although both fractions were more acidic in composition than calf thymus histones. However, the nucleolar histones were a metabolically distinct fraction, and their rate of synthesis was approximately twice that of the total nuclear histones.
mesh_terms: D000818:Animals; D002247:Carbon Isotopes; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002466:Cell Nucleolus / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D004586:Electrophoresis; D006657:Histones / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008113:Liver Neoplasms; D008239:Lysine / Q000378:metabolism; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D009374:Neoplasms, Experimental / Q000378:metabolism*; D011874:Radiometry; D051381:Rats
chemical_list: D002247:Carbon Isotopes; D006657:Histones; D009363:Neoplasm Proteins; D008239:Lysine