Use of polyelectrolytes and electron microscopy for detection of viruses from stool.
Insoluble polyelectrolytes (PE60) were used for the concentration of viruses from stool specimens, confirming the results of Wallis et al. (1969). Ten percent suspensions inoculated with poliovirus type 3 were used in these experiments. A small number of stool specimens from patients naturally infected with enteroviruses were also tested. Preferential adsorption of viruses to PE60 was maximum at a pH range of 4.5 to 6.0. The elution of the adsorbed viruses was optimal at pH 8.5. Other parameters were also investigated. Electron microscopy was used successfully to detect the eluted viruses.
D000327:Adsorption; D002021:Buffers; D004573:Electrolytes; D004770:Enterovirus / Q000302:isolation & purification*; D029822:Enterovirus B, Human / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008722:Methods; D008854:Microscopy, Electron; D010710:Phosphates; D017955:Poliovirus / Q000302:isolation & purification*; D013696:Temperature
D002021:Buffers; D004573:Electrolytes; D010710:Phosphates