Adenosine 3':5'-cyclic monophosphate and catabolite repression in Escherichia coli.
1. Both permanent and transient catabolite repression of beta-galactosidase synthesis in Escherichia coli are abolished by 5mm-3':5'-cyclic-AMP when elicited by glucose, but not when caused by a mixture of glucose, glucose 6-phosphate, gluconate and casein hydrolysate (casamino acids). 2. Glucose uptake is slightly increased by 3':5'-cyclic-AMP. 3. No significant effects of the nucleotide were found on the synthesis of protein and RNA, either in exponential growth on one substrate, or during a growth shift from glycerol to glycerol plus glucose. 4. Marked changes in the soluble-protein profiles of cells growing in glycerol and glucose were caused by the presence of 3':5'-cyclic-AMP. 5. Measurements of (14)CO(2) release from specifically-labelled glucose showed that 3':5'-cyclic-AMP greatly stimulated glycolytic activity while having a minor depressing effect on the metabolic flow through the pentose phosphate cycle. 6. The concentrations of several metabolic intermediates, particularly fructose 1,6-diphosphate, were greatly affected by the presence of 3':5'-cyclic-AMP. 7. Several metabolites partially relieved glucose repression of beta-galactosidase synthesis in EDTA-treated cells; three out of five of these metabolites reversed the effect more effectively than did 3':5'-cyclic-AMP. 8. The evidence for and against a direct role for 3':5'-cyclic-AMP is discussed. It is concluded that the evidence for indirect action is at least as strong as that for direct action.
D000227:Adenine Nucleotides / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000096:biosynthesis; D002245:Carbon Dioxide / Q000378:metabolism; D002247:Carbon Isotopes; D000242:Cyclic AMP / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology*; D005696:Galactosidases / Q000096:biosynthesis*; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects; D006600:Hexosephosphates / Q000378:metabolism; D010759:Phosphorus Isotopes; D012329:RNA, Bacterial / Q000096:biosynthesis
D000227:Adenine Nucleotides; D001426:Bacterial Proteins; D002247:Carbon Isotopes; D006600:Hexosephosphates; D010759:Phosphorus Isotopes; D012329:RNA, Bacterial; D002245:Carbon Dioxide; D004492:Edetic Acid; D000242:Cyclic AMP; D005696:Galactosidases; D005947:Glucose