Properties of a ribonucleoprotein particle isolated from Nonidet P-40-treated Rous sarcoma virus.
A ribonucleoprotein particle containing about 20% ribonucleic acid (RNA), and containing little if any phospholipid or glucosamine, was recovered in high yield after treatment of Schmidt-Ruppin strain of Rous sarcoma virus and B77 virus with the nonionic detergent Nonidet P-40. This structure, which probably derives from the internal ribonucleoprotein filament described in electron microscopy studies, contained 80 to 90% of the viral 60 to 70S RNA and only about 10% of the protein present in intact virions. It sedimented in glycerol density gradients at approximately 130S and had a buoyant density in sucrose of about 1.34 g/ml. Studies with (32)P-labeled virus indicated that the ribonucleoprotein particle contained approximately 30 4S RNA molecules per 10(7) daltons of high-molecular-weight viral RNA. Intact virions contained about 70 4S RNA molecules per 10(7) daltons of high-molecular-weight RNA. Electrophoretic studies in dodecyl sulfate-containing polyacrylamide gels showed that the ribonucleoprotein particle contained only 5 of the 11 polypeptides found in the virion; of these the major component was a polypeptide weighing 14,000 daltons.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000032:analysis* / Q000187:drug effects / Q000302:isolation & purification; D002247:Carbon Isotopes; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts; D008970:Molecular Weight; D009698:Nucleoproteins / Q000032:analysis* / Q000302:isolation & purification; D010455:Peptides / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D010759:Phosphorus Isotopes; D012367:RNA, Viral / Q000032:analysis; D013501:Surface-Active Agents / Q000494:pharmacology; D014316:Tritium; D014764:Viral Proteins / Q000032:analysis* / Q000302:isolation & purification
D002247:Carbon Isotopes; D009698:Nucleoproteins; D010455:Peptides; D010743:Phospholipids; D010759:Phosphorus Isotopes; D012367:RNA, Viral; D013501:Surface-Active Agents; D014764:Viral Proteins; D014316:Tritium