Site of action of 3',5'-cyclic adenosine monophosphate in production of tryptophanase in Escherichia coli.
3',5' cyclic-AMP (cAMP) will stimulate the rate of tryptophanase synthesis in Escherichia coli cultures induced with tryptophan. Adding cAMP after the initiation of messenger RNA synthesis was blocked by rifampicin, did not stimulate tryptophanase synthesis. This indicates that cAMP acts at initiation of either transcription or translation and not at the level of chain elongation of either the messenger or the polypeptide chain.
D000242:Cyclic AMP / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects*; D004926:Escherichia coli / Q000201:enzymology*; D006836:Hydro-Lyases / Q000096:biosynthesis*; D010442:Peptide Chain Initiation, Translational / Q000187:drug effects; D012333:RNA, Messenger / Q000037:antagonists & inhibitors; D012293:Rifampin / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors; D014364:Tryptophan / Q000494:pharmacology
D012333:RNA, Messenger; D014364:Tryptophan; D000242:Cyclic AMP; D006836:Hydro-Lyases; D012293:Rifampin