title: Catalytic properties and regulatory diversity of inorganic pyrophosphatases from photosynthetic bacteria.
abstract: Soluble inorganic pyrophosphatases of five species of nonsulfur purple bacteria were investigated in respect to reaction kinetics, regulatory behavior, and other characteristics. The enzymes appear to fall into two groups with correlated properties. The pyrophosphatases of Rhodopseudomonas capsulata and R. spheroides have molecular weights of approximately 60,000, are stabilized by Co(2+), and exhibit simple Michaelis-Menten reaction kinetics. On the other hand, the enzymes of R. palustris, R. gelatinosa, and Rhodospirillum rubrum are larger (molecular weight approximately 100,000), require Zn(2+) for maintenance of catalytic activity, and show complex reaction kinetics; these pyrophosphatases are activated by free Mg(2+) ions and, in the absence of the latter, are inhibited by 2-phosphoglyceric acid. The results described indicate the existence of alternative control patterns for regulation of intracellular turnover of phosphate, which is in part mediated by pyrophosphatases.
mesh_terms: D000645:Ammonium Sulfate; D002462:Cell Membrane / Q000201:enzymology; D002474:Cell-Free System; D002850:Chromatography, Gel; D003035:Cobalt / Q000378:metabolism; D003124:Colorimetry; D003470:Culture Media; D004789:Enzyme Activation; D005994:Glycerophosphates / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D008970:Molecular Weight; D010710:Phosphates / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000302:isolation & purification; D010788:Photosynthesis; D011755:Pyrophosphatases / Q000378:metabolism*; D012241:Rhodopseudomonas / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D012246:Rhodospirillum / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D013045:Species Specificity; D015032:Zinc / Q000378:metabolism
chemical_list: D003470:Culture Media; D005994:Glycerophosphates; D010710:Phosphates; D003035:Cobalt; D010744:Phosphoric Monoester Hydrolases; D011755:Pyrophosphatases; D008274:Magnesium; D015032:Zinc; D000645:Ammonium Sulfate