Deoxyribonucleic acid polymerase of Rous sarcoma virus: studies on the mechanism of double-stranded deoxyribonucleic acid synthesis.
The deoxyribonucleic acid (DNA) polymerase of Rous sarcoma virus synthesizes both single- and double-stranded DNA, utilizing the ribonucleic acid (RNA) of the viral genome as the initial template. Results of pulse-chase experiments indicate that the single-stranded DNA serves as unconserved template and precursor for the synthesis of double-stranded DNA. The latter reaction is apparently initiated in association with the viral RNA and may involve a partially double-stranded intermediate form.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D002501:Centrifugation, Zonal; D002642:Chick Embryo; D046508:Culture Techniques; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004279:DNA, Viral / Q000096:biosynthesis* / Q000302:isolation & purification; D005347:Fibroblasts; D005815:Genetic Code; D005827:Genetics, Microbial; D006882:Hydroxyapatites; D008962:Models, Theoretical; D009711:Nucleotides / Q000378:metabolism; D012367:RNA, Viral / Q000302:isolation & purification; D012260:Ribonucleases / Q000494:pharmacology; D013395:Sucrose; D013698:Templates, Genetic; D014316:Tritium
D004279:DNA, Viral; D006882:Hydroxyapatites; D009711:Nucleotides; D012367:RNA, Viral; D014316:Tritium; D013395:Sucrose; D004254:DNA Nucleotidyltransferases; D012260:Ribonucleases