Analysis of the events leading to SV40-induced chromosome replication and mitosis in primary mouse kidney cell cultures.
Abortive infection with simian virus 40 in confluent, "contact-inhibited", mouse kidney cell cultures was studied. The sequential events, in individual cells, are tentatively represented by the simplified scheme: (a) transcription of early virus-specific (messenger) RNA; (b) appearance of T-antigen; (c) [psychrosensitive event(s)]; (d) chromosome replication; (e) normal or abnormal mitosis. No evidence for the replication of viral progeny DNA was obtained. The sequence of the events (a)-(d) is analogous to that observed in contact-inhibited mouse kidney tissue culture cells during lytic infection with polyoma virus.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001345:Autoradiography; D002478:Cells, Cultured / Q000378:metabolism* / Q000382:microbiology; D002875:Chromosomes; D003260:Contact Inhibition; D004247:DNA / Q000032:analysis; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis; D005455:Fluorescent Antibody Technique; D007668:Kidney / Q000166:cytology; D051379:Mice; D008938:Mitosis; D013539:Simian virus 40 / Q000276:immunology; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium
D000956:Antigens, Viral; D004279:DNA, Viral; D014316:Tritium; D004247:DNA; D013936:Thymidine