Medium for toxin production by Clostridium perfringens in continuous culture.
A tryptone-salts medium for continuous growth and alpha toxin production of Clostridium perfringens type A in an adapted chemostat is described. In such steady-state cultures, fermentative and biochemical activity of C. perfringens remained unchanged. Toxigenic ability to produce alpha, theta, and nu toxins was preserved.
D001431:Bacteriological Techniques; D003016:Clostridium perfringens / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media; D005285:Fermentation; D008722:Methods; D014118:Toxins, Biological / Q000032:analysis / Q000096:biosynthesis*
D003470:Culture Media; D014118:Toxins, Biological