Detection and assay of avian tumor virus group-specific antigen and antibody by the paired radioiodine-labeled antibody technique.
The paired radioiodine-labeled antibody technique (PRILAT) was applied to the detection and quantitation of avian tumor virus group-specific (gs) antigens and antibody. The technique proved to be specific, repeatable, and appreciably more sensitive than the microcomplement-fixation test for avian leukosis (COFAL). The PRILAT facilitated direct measurement of comparative antigen content of several types of transformed, neoplastic, or virus-infected cells and the magnitude of nonspecific antibody binding by appropriate control cells. The versatility of the technique was illustrated by application to the detection and quantitation of gs antibody content of chicken, turkey, pigeon, and hamster sera. Antibodies were detected in COFAL-negative sera from hamsters bearing tumors induced by the Schmidt-Ruppin strain of Rous sarcoma virus. Sera from chickens bearing similar tumors were not positive for gs antibodies, although sera from turkeys and chickens immunized with avian leukosis virus did contain gs antibodies.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis; D001354:Avian Leukosis Virus / Q000276:immunology*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000276:immunology; D002642:Chick Embryo; D002645:Chickens / Q000276:immunology; D010856:Columbidae / Q000276:immunology; D006224:Cricetinae / Q000276:immunology; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D007074:Immunoglobulin G / Q000032:analysis; D007456:Iodine Isotopes; D007668:Kidney; D001357:Sarcoma, Avian / Q000276:immunology; D014422:Turkeys / Q000276:immunology
D000906:Antibodies; D000941:Antigens; D000956:Antigens, Viral; D007106:Immune Sera; D007074:Immunoglobulin G; D007456:Iodine Isotopes