Mutants of Escherichia coli K-12 blocked in the final reaction of ubiquinone biosynthesis: characterization and genetic analysis.
The ubiquinone precursor 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1, 4-benzoquinone was isolated from two ubiquinone-deficient mutants of Escherichia coli and identified by nuclear magnetic resonance and mass spectrometry. The results of genetic analysis of the mutants indicate that each mutant carries a mutation in a gene designated ubiG which was located, by cotransduction with the nalA and glpT genes, at minute 42 on the E. coli chromosome.
D002474:Cell-Free System; D055598:Chemical Phenomena; D002621:Chemistry; D002855:Chromatography, Thin Layer; D002874:Chromosome Mapping; D003227:Conjugation, Genetic; D003470:Culture Media; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D005782:Gels; D005796:Genes; D005947:Glucose / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009153:Mutagens; D009154:Mutation; D009268:Nalidixic Acid / Q000494:pharmacology; D009604:Nitrosoguanidines; D011809:Quinones / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification; D012822:Silicon Dioxide; D013053:Spectrophotometry; D013386:Succinates / Q000378:metabolism; D014161:Transduction, Genetic; D014451:Ubiquinone / Q000096:biosynthesis*; D014466:Ultraviolet Rays
D003470:Culture Media; D005782:Gels; D009153:Mutagens; D009604:Nitrosoguanidines; D011809:Quinones; D013386:Succinates; D014451:Ubiquinone; D009268:Nalidixic Acid; D012822:Silicon Dioxide; D005947:Glucose