Rapid assay for hormone-sensitive lipase activity of adipose tissue.
A highly specific and rapid assay for hormone-sensitive lipase activity of rat adipose tissue is described. The method employs emulsified 2,3-di-O-oleyl-[9,10-(3)H(2)]oleoyl glycerol as a substrate; it is very sensitive and is suitable for serial sampling.
D000255:Adenosine Triphosphate; D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D002110:Caffeine; D055598:Chemical Phenomena; D002621:Chemistry; D000242:Cyclic AMP; D004789:Enzyme Activation; D004950:Esterases; D004987:Ethers / Q000138:chemical synthesis; D005989:Glycerides / Q000138:chemical synthesis; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008049:Lipase / Q000032:analysis*; D008071:Lipoprotein Lipase; D008274:Magnesium; D009829:Oleic Acids; D051381:Rats; D014280:Triglycerides; D014316:Tritium
D004987:Ethers; D005989:Glycerides; D009829:Oleic Acids; D014280:Triglycerides; D014316:Tritium; D002110:Caffeine; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D004950:Esterases; D008049:Lipase; D008071:Lipoprotein Lipase; D008274:Magnesium