title: Effect of benzo(a) pyrene and piperonyl butoxide on formation of respiratory system, phospholipids, and carotenoids of Staphylococcus aureus.
abstract: Staphylococcus aureus formed an electron transport system when exponentially growing cells were aerated. Formation of the electron transport system occurred concomitantly with increases in the phospholipids and the carotenoids. The addition of piperonyl butoxide or benzo(a)pyrene at the onset of aeration (i) slowed the formation of the electron transport system, (ii) both inhibited cytochrome oxidase o synthesis and decreased its stability, (iii) simultaneously depressed the increase in total phospholipid (especially cardiolipin), and (iv) depressed the synthesis of the carotenoid rubixanthin. Benzo(a)pyrene was the more inhibitory of the two, both on the rate of synthesis of the electron transport system and on rubixanthin formation. Evidence obtained with the inhibitors suggested that inhibition of the lipid synthesis was related to the formation of the electron transport system.
mesh_terms: D001580:Benzopyrenes / Q000494:pharmacology*; D002338:Carotenoids / Q000096:biosynthesis*; D002854:Chromatography, Paper; D003124:Colorimetry; D003470:Culture Media; D003580:Cytochromes / Q000378:metabolism; D004149:Dioxoles / Q000494:pharmacology*; D004579:Electron Transport / Q000187:drug effects*; D003576:Electron Transport Complex IV / Q000096:biosynthesis; D005782:Gels; D007306:Insecticides / Q000494:pharmacology*; D008055:Lipids / Q000032:analysis / Q000302:isolation & purification; D010100:Oxygen; D010743:Phospholipids / Q000096:biosynthesis*; D010759:Phosphorus Isotopes; D012822:Silicon Dioxide; D013053:Spectrophotometry; D013210:Staphylococcus / Q000032:analysis / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*
chemical_list: D001580:Benzopyrenes; D003470:Culture Media; D003580:Cytochromes; D004149:Dioxoles; D005782:Gels; D007306:Insecticides; D008055:Lipids; D010743:Phospholipids; D010759:Phosphorus Isotopes; D002338:Carotenoids; D012822:Silicon Dioxide; D003576:Electron Transport Complex IV; D010100:Oxygen