Structural proteins of simian virus 40: phosphoproteins.
All five structural polypeptides of infectious simian virus 40 grown in African green monkey kidney cells were found to be phosphorylated. The polypeptides with the largest and smallest molecular weights are phosphorylated to a somewhat lower extent than the other polypeptides. The protein moiety of "empty" virus, which is essentially devoid of deoxyribonucleic acid, exhibited a degree of phosphorylation similar to that of infectious virus. In the major polypeptide (molecular weight: 49,000), the phosphate appears to be bound to the seryl or threonyl residues, or both. The nature of the phosphate-polypeptide bond in the other viral polypeptides remains obscure.
D000596:Amino Acids; D000818:Animals; D002247:Carbon Isotopes; D002460:Cell Line; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D003851:Deoxyribonucleases; D004591:Electrophoresis, Polyacrylamide Gel; D000882:Haplorhini; D007668:Kidney; D008970:Molecular Weight; D010085:Oxidative Phosphorylation; D010455:Peptides / Q000032:analysis; D010636:Phenols; D010710:Phosphates / Q000378:metabolism; D010750:Phosphoproteins / Q000032:analysis*; D010727:Phosphoric Diester Hydrolases; D010759:Phosphorus Isotopes; D010770:Phosphotransferases / Q000378:metabolism; D013539:Simian virus 40 / Q000032:analysis* / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification; D013936:Thymidine; D014316:Tritium; D014764:Viral Proteins / Q000032:analysis*
D000596:Amino Acids; D002247:Carbon Isotopes; D010455:Peptides; D010636:Phenols; D010710:Phosphates; D010750:Phosphoproteins; D010759:Phosphorus Isotopes; D014764:Viral Proteins; D014316:Tritium; D010770:Phosphotransferases; D003851:Deoxyribonucleases; D010727:Phosphoric Diester Hydrolases; D013936:Thymidine