title: Biochemically differentiated neoplastic clone of Schwann cells.
abstract: Four clonal lines have been established in tissue culture from a transplantable tumor of cervical nerve-root induced transplacentally with ethylnitrosourea in an inbred BD-IX rat strain. One clone (RN-2) has several biochemical properties of the nervous system that led to its classification as a Schwann cell line, namely (a) the synthesis of the nervous system specific protein S-100, (b) a high specific activity of 2',3'-cyclic nucleotide-3'-phosphohydrolase, an enzyme present in high specific activity only in glial cells, and (c) the presence of a basic protein related by immunological crossreaction, molecular size, and amino-acid composition to the encephalitogenic protein from beef brain myelin. Clone RN-2 has a high plating efficiency, a doubling time of about 20 hr, and a mean of 43 chromosomes of normal morphology. Clone RN-2 has also been inoculated subcutaneously into syngeneic rats and grown as a clonal tumor with good growth properties.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002454:Cell Differentiation; D002471:Cell Transformation, Neoplastic; D002875:Chromosomes / Q000032:analysis; D002999:Clone Cells / Q000378:metabolism*; D003168:Complement Fixation Tests; D003429:Cross Reactions; D046508:Culture Techniques; D000242:Cyclic AMP; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D009186:Myelin Sheath; D009368:Neoplasm Transplantation; D009419:Nerve Tissue Proteins / Q000032:analysis; D009442:Neurilemmoma / Q000139:chemically induced; D009607:Nitrosourea Compounds; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D012583:Schwann Cells / Q000032:analysis / Q000201:enzymology / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000139:chemically induced*
chemical_list: D000596:Amino Acids; D009419:Nerve Tissue Proteins; D009607:Nitrosourea Compounds; D000242:Cyclic AMP; D010744:Phosphoric Monoester Hydrolases