Developing neuromuscular junctions: first signs of chemical transmission during formation in tissue culture.
Electrophysiological study of rat neuromuscular junctions in the early stage of formation in tissue culture showed that chemical transmission begins with discrete, localized release of transmitter about the time when nerve-muscle contacts are first visible with light microscopy. Noncontractile myotubes with as few as three nuclei showed evidence of junctional transmission.
D000109:Acetylcholine / Q000502:physiology; D000818:Animals; D046508:Culture Techniques; D005071:Evoked Potentials; D007365:Intercellular Junctions; D009469:Neuromuscular Junction / Q000196:embryology* / Q000502:physiology*; D051381:Rats; D013116:Spinal Cord / Q000196:embryology / Q000502:physiology; D009435:Synaptic Transmission
D000109:Acetylcholine