Polyelectrolyte flocculation as an aid to recovery of enteroviruses from oysters.
A simple and rapid method for recovering enteroviruses from oysters is described. A polycation sewage flocculant promoted cohesion of oyster solids and thereby aided separation of these from the viruses. Recovery of 80 to 100% of experimentally inoculated virus was achieved, and the suspension or extract obtained could be inoculated directly into tissue cultures or concentrated first for greater sensitivity.
D000906:Antibodies; D004573:Electrolytes; D004770:Enterovirus / Q000302:isolation & purification*; D029822:Enterovirus B, Human / Q000302:isolation & purification*; D005243:Feces / Q000276:immunology; D005427:Flocculation; D005506:Food Contamination; D005516:Food Microbiology; D008722:Methods; D009500:Neutralization Tests; D010125:Ostreidae; D017955:Poliovirus / Q000276:immunology / Q000302:isolation & purification*; D000644:Quaternary Ammonium Compounds; D014020:Tissue Extracts; D014461:Ultracentrifugation
D000906:Antibodies; D004573:Electrolytes; D000644:Quaternary Ammonium Compounds; D014020:Tissue Extracts