title: Interaction of dehydroheliotridine, a metabolite of heliotridine-based pyrrolizidine alkaloids, with native and heat-denatured deoxyribonucleic acid in vitro.
abstract: Dehydroheliotridine, a major pyrrolic metabolite of heliotridine-based pyrrolizidine alkaloids, interacted with both native and heat-denatured DNA in vitro under mildly acid conditions to form a soluble non-diffusible complex as shown by spectral data. The complex formed with heat-denatured DNA was more resistant than heat-denatured DNA alone to the combined action of deoxyribonuclease and phosphodiesterase. Analysis of the resistant fractions suggested the presence of at least one base not obtained from DNA alone.
mesh_terms: D000470:Alkaloids; D055598:Chemical Phenomena; D002621:Chemistry; D002845:Chromatography; D004247:DNA; D003851:Deoxyribonucleases; D006571:Heterocyclic Compounds; D046911:Macromolecular Substances; D009691:Nucleic Acid Denaturation; D010744:Phosphoric Monoester Hydrolases; D013057:Spectrum Analysis
chemical_list: D000470:Alkaloids; D006571:Heterocyclic Compounds; D046911:Macromolecular Substances; D004247:DNA; D003851:Deoxyribonucleases; D010744:Phosphoric Monoester Hydrolases