Quantitation of Clostridium perfringens type A enterotoxin by electroimmunodiffusion.
Conditions for quantitation of Clostridium perfringens type A enterotoxin by electroimmunodiffusion are described. As little as 0.01 mug of enterotoxin could be detected. Electroimmunodiffusion was more sensitive than either single gel diffusion or quantitation based on erythemal activity of the toxin in guinea pig skin.
D000362:Agar; D000818:Animals; D001431:Bacteriological Techniques; D002852:Chromatography, Ion Exchange; D003016:Clostridium perfringens / Q000032:analysis*; D004586:Electrophoresis; D004768:Enterotoxins / Q000032:analysis* / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D006168:Guinea Pigs; D005779:Immunodiffusion; D012703:Serotyping; D012882:Skin Tests
D004768:Enterotoxins; D000362:Agar