A protein binding assay for adenosine 3':5'-cyclic monophosphate.
A simple and sensitive assay for adenosine 3':5'-cyclic monophosphate (cAMP) has been developed that is based on competition for protein binding of the nucleotide, presumably to a cAMP-dependent protein kinase. The nucleotide-protein complex is adsorbed on a cellulose ester filter. Assay conditions are such that a binding constant approaching 10(-9) M is obtained, and the assay is thus sensitive to 0.05-0.10 pmol of cAMP.
D000227:Adenine Nucleotides / Q000032:analysis*; D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002482:Cellulose; D002848:Chromatography, DEAE-Cellulose; D000242:Cyclic AMP / Q000032:analysis; D005374:Filtration; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000032:analysis; D008722:Methods; D051379:Mice; D009132:Muscles / Q000201:enzymology; D010770:Phosphotransferases; D011485:Protein Binding; D011506:Proteins; D014316:Tritium
D000227:Adenine Nucleotides; D011506:Proteins; D014316:Tritium; D002482:Cellulose; D000242:Cyclic AMP; D010770:Phosphotransferases