Radioreceptor assay of adrenocorticotropic hormone: new approach to assay of polypeptide hormones in plasma.
Biologically active iodine-125-labeled adrenocorticotropic hormone (ACTH) binds specifically to ACTH receptors extracted from adrenals. Unlabeled ACTH at 1 picogram per milliliter significantly displaces labeled ACTH from these receptors. This system, which appears to be applicable to all polypeptide hormones, provides a rapid and sensitive method for measurements of biologically active ACTH in dilute whole plasma.
D000311:Adrenal Glands / Q000187:drug effects*; D000324:Adrenocorticotropic Hormone / Q000097:blood*; D000818:Animals; D001681:Biological Assay; D003480:Cushing Syndrome / Q000097:blood; D006728:Hormones / Q000097:blood; D006801:Humans; D007018:Hypopituitarism / Q000097:blood; D007456:Iodine Isotopes; D008722:Methods; D010455:Peptides / Q000097:blood; D011863:Radioimmunoassay; D011955:Receptors, Drug; D013552:Swine
D006728:Hormones; D007456:Iodine Isotopes; D010455:Peptides; D011955:Receptors, Drug; D000324:Adrenocorticotropic Hormone