Sequence of spontaneous Epstein-Barr virus activation and selective DNA synthesis in activated cells in the presence of hydroxyurea.
The sequence of spontaneous Epstein-Barr virus activation was studied in P3HR-1 carrier cells and in P3HR-1(BrdU) cells made resistant to 5-bromodeoxyuridine. Virus activation was initiated during the normal cell cycle, and recruitment of additional virus-activated cells was prevented by the DNA inhibitors, 1-beta-D-arabinofuranosylcytosine and hydroxyurea. Virus activation was followed by synthesis of the early antigen complex in the absence of additional detectable DNA synthesis. Early antigen synthesis was followed by hydroxyurea-resistant synthesis of new DNA, which in the case of P3HR-1(BrdU) cells was characterized by the appearance of thymidine kinase. The newly synthesized DNA banded in neutral cesium chloride at peaks corresponding to normal human DNA and Epstein-Barr viral DNA. Synthesis of viral antigen was seen only in cells that had undergone hydroxyurea-resistant DNA synthesis.
D000225:Adenine / Q000378:metabolism; D000956:Antigens, Viral / Q000032:analysis; D001345:Autoradiography; D001973:Bromodeoxyuridine / Q000378:metabolism; D002247:Carbon Isotopes; D002277:Carcinoma / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003561:Cytarabine / Q000494:pharmacology; D004279:DNA, Viral / Q000096:biosynthesis*; D005455:Fluorescent Antibody Technique; D006564:Herpesviridae; D004854:Herpesvirus 4, Human / Q000201:enzymology / Q000254:growth & development; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology*; D009303:Nasopharyngeal Neoplasms / Q000276:immunology; D013936:Thymidine / Q000378:metabolism; D013937:Thymidine Kinase / Q000096:biosynthesis; D014316:Tritium; D014779:Virus Replication
D000956:Antigens, Viral; D002247:Carbon Isotopes; D004279:DNA, Viral; D003561:Cytarabine; D014316:Tritium; D013937:Thymidine Kinase; D001973:Bromodeoxyuridine; D000225:Adenine; D013936:Thymidine; D006918:Hydroxyurea