RNA-primed DNA synthesis in vitro.
In vitro DNA synthesis on single-stranded circular DNA can be initiated by RNA primers. RNA chains are covalently extended by DNA polymerase II from KB cells and DNA polymerase I from Micrococcus luteus, but not by an RNA-dependent DNA polymerase from avian myeloblastosis virus. The reaction product consists of DNA chains with a piece of RNA at their 5'-ends, hydrogen bonded to the template DNA. The primer RNA is linked to the product DNA via a 3':5'-phosphodiester bond, and can be specifically removed by ribonuclease H. The possible role of ribonuclease H in RNA-primed DNA synthesis in vivo is discussed.
D001354:Avian Leukosis Virus / Q000201:enzymology; D001665:Binding Sites; D002277:Carcinoma; D002460:Cell Line / Q000201:enzymology; D002499:Centrifugation, Density Gradient; D003090:Coliphages; D004254:DNA Nucleotidyltransferases; D004277:DNA, Single-Stranded; D004279:DNA, Viral / Q000138:chemical synthesis*; D004926:Escherichia coli / Q000201:enzymology; D006801:Humans; D008837:Micrococcus / Q000201:enzymology; D009062:Mouth Neoplasms; D009491:Neurospora / Q000201:enzymology; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D011485:Protein Binding; D012316:RNA Nucleotidyltransferases; D012367:RNA, Viral / Q000138:chemical synthesis; D013698:Templates, Genetic
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D012367:RNA, Viral; D004254:DNA Nucleotidyltransferases; D012316:RNA Nucleotidyltransferases