title: Physiological concentrations of lactate dehydrogenases and substrate inhibition.
abstract: Lactate dehydrogenases at physiological concentrations are inhibited by high concentrations of pyruvate when the enzyme and the pyruvate are incubated in the presence of oxidized nicotinamide-adenine dinucleotide before assay. The inhibition is much more pronounced with the H-type than with the M-type lactate dehydrogenase. These results suggest that substrate inhibition may be operative in vivo.
mesh_terms: D000818:Animals; D002645:Chickens; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors*; D008722:Methods; D009243:NAD / Q000494:pharmacology; D011773:Pyruvates / Q000494:pharmacology*
chemical_list: D007527:Isoenzymes; D011773:Pyruvates; D009243:NAD; D007770:L-Lactate Dehydrogenase