An enzymically catalysed reaction between D-glucose and the protein cyst coat of the ciliate Colpoda steinii.
1. The isolated protein cyst coat of Colpoda steinii reacted with [(14)C]glucose to bind (14)C label in a reaction that was not an artifact of bacterial contamination or of adsorption. 2. The reaction was enzymically catalysed, had optimum pH7.0-7.4 and a temperature optimum of 36-38 degrees C, was non-competitively and reversibly inhibited by 10mum-p-hydroxymercuribenzoate and was specific for d-glucose. It had K(m)8.2x10(-6)m. 3. With the exception of C-1, all the carbon atoms of glucose were bound to the same extent and, measured relative to C-2 or C-6, the binding of C-1 varied between 0.45 and 0.82. The lost C-1 was not accounted for as carbon dioxide. 4. On prolonged incubation a coat preparation reacted with 3.6% of its own weight of glucose. 5. The label was tightly bound, but after acid treatment a variable proportion was recovered as glucose and there was no evidence for the release of any other (14)C-containing compound. 6. Even after dissolution of the coat protein, bound label was not removed by treatment with periodate or lead tetra-acetate.
D000085:Acetates / Q000494:pharmacology; D000818:Animals; D002247:Carbon Isotopes; D002473:Cell Wall / Q000378:metabolism*; D016798:Ciliophora / Q000201:enzymology*; D005947:Glucose / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007854:Lead / Q000494:pharmacology; D008628:Mercury / Q000494:pharmacology; D009942:Organometallic Compounds / Q000494:pharmacology; D010504:Periodic Acid / Q000494:pharmacology; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D013696:Temperature
D000085:Acetates; D002247:Carbon Isotopes; D009942:Organometallic Compounds; D011506:Proteins; D010504:Periodic Acid; D007854:Lead; D008628:Mercury; D005947:Glucose