Efficient filtration and sizing of viruses with membrane filters.
Untreated membrane filters retain viruses by adsorption, as well as by physical restriction which occurs when the pore diameter of the filter is smaller than that of the virus particle. As originally recommended by Elford, membranes had to be pretreated with proteinaceous material to preclude virus adsorption. However, coating materials that prevent adsorption of certain viruses do not necessarily prevent adsorption of other viruses. In contrast to proteins, salts enhance virus adsorption. Viruses treated with sodium lauryl sulfate to reduce the surface tension, or purified viruses in distilled water, are not adsorbed to membranes. A procedure is recommended by which viruses may be passed through membranes with a porosity twice the diameter of the virus. Such filtrates, which contain 50 to 100% of the initial virus concentration, should be used for sizing viruses by subsequent filtration through smaller pores. The determination of virus size would then be based on the major population of particles in the virus suspension. In the past, as little as 0.1 to 0.001% of the initial virus population was the basis for size determination, because more than 99.9% of the virus was often lost by adsorption to membranes during the clarifying procedures.
D000327:Adsorption; D000818:Animals; D002460:Cell Line; D005374:Filtration; D000882:Haplorhini; D006564:Herpesviridae / Q000302:isolation & purification; D007668:Kidney; D008567:Membranes, Artificial; D017955:Poliovirus / Q000302:isolation & purification; D012412:Rubella virus / Q000302:isolation & purification; D012492:Salts / Q000494:pharmacology; D014616:Vaccinia virus / Q000302:isolation & purification; D014780:Viruses / Q000254:growth & development / Q000302:isolation & purification*
D008567:Membranes, Artificial; D012492:Salts