Integration of simian virus 40 deoxyribonucleic acid into the deoxyribonucleic acid of primary infected Chinese hamster cells.
Simian virus 40 deoxyribonucleic acid (DNA) became associated in an alkaline-stable form with the DNA of Chinese hamster embryo cells at 15 to 20 hr post-infection, at the time when cell DNA synthesis and T antigen were induced. The integration process was not inhibited by d-arabinosyl cytosine and was only partially inhibited by cycloheximide.
D000818:Animals; D000956:Antigens, Viral / Q000378:metabolism; D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis / Q000378:metabolism; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D046508:Culture Techniques; D003513:Cycloheximide / Q000494:pharmacology; D003561:Cytarabine / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D007930:Leucine / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral; D013539:Simian virus 40 / Q000276:immunology / Q000378:metabolism / Q000472:pathogenicity*; D013395:Sucrose; D013501:Surface-Active Agents; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium; D014633:Valine / Q000378:metabolism
D000956:Antigens, Viral; D004279:DNA, Viral; D012367:RNA, Viral; D013501:Surface-Active Agents; D003561:Cytarabine; D014316:Tritium; D013395:Sucrose; D004247:DNA; D003513:Cycloheximide; D007930:Leucine; D014633:Valine; D008239:Lysine; D013936:Thymidine