Virus-specific antigens in hamster cells transformed by Rous sarcoma virus.
Virus-specific antigens were studied in hamster cells transformed by Rous sarcoma virus (RSV). Antigens were localized in the cytoplasm, as demonstrated by fluorescent antibody staining of fixed cells as well as by complement fixation (CF) following subcellular fractionation. Cytoplasmic extracts were analyzed by velocity and isopycnic centrifugation. CF antigens were found in a soluble form and in association with membranes and polyribosomes. Isolated plasma membranes had no CF antigen. Both soluble and particulate fractions with CF activity contained the same antigenic determinants by Ouchterlony analysis. These antigenic determinants were identical to those released by ether treatment of RSV.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D001354:Avian Leukosis Virus / Q000276:immunology; D001358:Avian Sarcoma Viruses / Q000276:immunology*; D002460:Cell Line; D002462:Cell Membrane / Q000032:analysis / Q000276:immunology; D002471:Cell Transformation, Neoplastic; D002499:Centrifugation, Density Gradient; D003168:Complement Fixation Tests; D006224:Cricetinae; D046508:Culture Techniques; D003593:Cytoplasm / Q000276:immunology; D004247:DNA / Q000032:analysis; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D005779:Immunodiffusion; D007668:Kidney; D010743:Phospholipids / Q000032:analysis; D011506:Proteins / Q000032:analysis; D012313:RNA / Q000032:analysis; D011817:Rabbits; D012270:Ribosomes / Q000276:immunology; D013395:Sucrose
D000941:Antigens; D007106:Immune Sera; D010743:Phospholipids; D011506:Proteins; D013395:Sucrose; D012313:RNA; D004247:DNA