Linear, single-stranded deoxyribonucleic acid isolated from Kilham rat virus.
Kilham rat virus (KRV) was grown in a rat nephroma cell line and was purified by two isopycnic centrifugations in cesium chloride. The virus contains single-stranded deoxyribonucleic acid (DNA) with a molecular weight of approximately 1.6 x 10(6). The DNA was extracted from the virion by both phenol extraction and by 2% sodium dodecyl sulfate at 50 C. KRV DNA, extracted by both procedures, was observed in an electron microscope by using a cytochrome c or diethylaminoethyldextran monolayer. The DNA was also exposed to exonuclease I, an enzyme which hydrolyzes specifically linear, single-stranded DNA. Hydrolysis of 70 to 80% of the DNA was observed. Both the enzymatic and the electron microscope studies support the conclusion that extracted KRV DNA is a single-stranded, linear molecule. The length of the DNA was measured in the electron microscope and determined to be 1.505 +/- 0.206 mum.
D000818:Animals; D002247:Carbon Isotopes; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D003580:Cytochromes; D004279:DNA, Viral / Q000302:isolation & purification; D003851:Deoxyribonucleases / Q000494:pharmacology; D004492:Edetic Acid; D006868:Hydrolysis; D008854:Microscopy, Electron; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D010636:Phenols; D051381:Rats; D012964:Sodium; D012997:Solvents; D013395:Sucrose; D013431:Sulfates; D013936:Thymidine; D014316:Tritium; D014776:Virus Cultivation; D014713:Viruses, Unclassified / Q000032:analysis* / Q000302:isolation & purification; D009396:Wilms Tumor
D002247:Carbon Isotopes; D002712:Chlorides; D003580:Cytochromes; D004279:DNA, Viral; D010636:Phenols; D012997:Solvents; D013431:Sulfates; D014316:Tritium; D002586:Cesium; D013395:Sucrose; D004492:Edetic Acid; D012964:Sodium; D003851:Deoxyribonucleases; D013936:Thymidine