Immunological relationships of reverse transcriptases from ribonucleic acid tumor viruses.
Antiserum to partially purified reverse transcriptase from the Schmidt-Ruppin strain of Rous sarcoma virus has been prepared and characterized. Antibody to the avian polymerase inhibited the reverse transcriptase activity of avian C-type viruses but had no effect on the polymerase activity from C-type viruses of other classes. The known mammalian C-type viral polymerases were significantly inhibited only by the antiserum to murine C-type viral polymerases; reverse transcriptases from four other mammalian viruses were immunologically distinct from both avian and mammalian C-type viral polymerases. Partially purified murine leukemia viral DNA polymerase activity was comparably reduced by specific antibody regardless of the template used for enzyme detection.
D000818:Animals; D000918:Antibody Specificity; D001358:Avian Sarcoma Viruses / Q000201:enzymology* / Q000276:immunology; D002474:Cell-Free System; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003429:Cross Reactions; D004254:DNA Nucleotidyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D007106:Immune Sera; D007074:Immunoglobulin G; D009052:Leukemia Virus, Murine / Q000201:enzymology; D009858:Oncogenic Viruses / Q000201:enzymology* / Q000276:immunology; D011328:Prions / Q000201:enzymology* / Q000276:immunology; D012328:RNA Viruses / Q000201:enzymology* / Q000276:immunology; D011817:Rabbits; D013045:Species Specificity; D013698:Templates, Genetic; D013942:Thymine Nucleotides / Q000378:metabolism; D014316:Tritium
D007106:Immune Sera; D007074:Immunoglobulin G; D011328:Prions; D013942:Thymine Nucleotides; D014316:Tritium; D004254:DNA Nucleotidyltransferases