1,25-dihydroxycholecalciferol: a potent stimulator of bone resorption in tissue culture.
1,25-Dihydroxycholecalciferol (DHCC), isolated from kidney homogenates incubated with 25-hydroxycholecalciferol (HCC), stimulated the release of previously incorporated (45)45Ca from fetal rat bones in organ culture, at concentrations of 10(-10) to 10(-8)M. The dose response curves for 1,25-DHCC and 25-HCC, the parent compound, are parallel, but 1,25-DHCC is about 100 times as potent on a weight basis. Brief exposure to maximum doses of either agent leads to prolonged bone resorption.
D000818:Animals; D001345:Autoradiography; D001862:Bone Resorption / Q000187:drug effects*; D001842:Bone and Bones / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002127:Calcium Isotopes; D002762:Cholecalciferol / Q000494:pharmacology*; D046508:Culture Techniques; D006887:Hydroxycholecalciferols / Q000302:isolation & purification / Q000494:pharmacology; D007668:Kidney / Q000032:analysis; D051381:Rats; D013268:Stimulation, Chemical; D014316:Tritium
D002127:Calcium Isotopes; D006887:Hydroxycholecalciferols; D014316:Tritium; D002762:Cholecalciferol; D002118:Calcium