The time-course of the effects of ethanol on the redox and phosphorylation states of rat liver.
1. The time-course of the effects of ethanol administration on the metabolite concentrations, redox states and phosphorylation state was studied in the freeze-clamped liver of starved rats. The response was found to vary with the time after ethanol administration. 2. Administration of ethanol caused an immediate decrease in the [NAD(+)]/[NADH] ratio of both cytoplasm and mitochondria, which persisted over the 30min studied. 3. The free cytoplasmic [NADP(+)]/[NADPH] ratio in liver decreases immediately after ethanol administration but returns nearly to control values after 15min. 4. The cytoplasmic [ATP]/[ADP][HPO(4) (2-)] ratio is elevated 15min after ethanol administration in the starved rat. 5. The rapid and large changes in most metabolite concentrations measured appeared to result from the maintenance of near-equilibrium in a wide interlinked network. 6. Differences between fed and starved rats 15min after ethanol administration were slight.
D000244:Adenosine Diphosphate / Q000032:analysis; D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D003593:Cytoplasm / Q000032:analysis; D000431:Ethanol / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D008930:Mitochondria, Liver / Q000032:analysis; D009243:NAD / Q000032:analysis; D010084:Oxidation-Reduction; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D051381:Rats; D013217:Starvation / Q000378:metabolism; D013997:Time Factors
D009243:NAD; D000431:Ethanol; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate