Rat liver alcohol dehydrogenase. Purification and properties.
Alcohol dehydrogenase (EC 1.1.1.1) from the rat liver supernatant fraction has been purified 200-fold and partially characterized. The isolation procedure involved ammonium sulphate fractionation, DEAE-Sephadex chromatography and gel filtration. The purified enzyme behaved as a homogeneous preparation as evaluated by cellulose acetate and polyacrylamide-gel disc electrophoresis. Sulphoethyl-Sephadex chromatography and immunoelectrophoresis with rabbit antiserum indicated the presence of a minor component. Rat liver alcohol dehydrogenase appears to contain 4mol of zinc/mol, has an estimated molecular weight of 65000 and consists of two subunits of similar molecular weight. Heavy-metal ions, thiol-blocking reagents, urea at concentrations below 8m, low pH (5.5) and chelating agents deactivate the enzyme but do not dissociate it into subunits. Deactivated enzyme could not be reactivated. The enzyme is strictly specific for NAD(+) and has a broad specificity for alcohols, which are bound at a hydrophobic site. Inhibition occurred with the enzyme equilibrated with Zn(2+) at concentrations above 0.1mm.
D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D000818:Animals; D001665:Binding Sites; D002614:Chelating Agents / Q000494:pharmacology; D002845:Chromatography; D002850:Chromatography, Gel; D004586:Electrophoresis; D004589:Electrophoresis, Disc; D007122:Immunoelectrophoresis; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D009243:NAD; D051381:Rats; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology; D015032:Zinc
D002614:Chelating Agents; D013439:Sulfhydryl Reagents; D009243:NAD; D014508:Urea; D000429:Alcohol Oxidoreductases; D015032:Zinc