Microculture plaque assay for human and simian cytomegaloviruses.
The plaque assay for human and simian cytomegaloviruses routinely carried out in 60-mm petri dishes (macrocultures) has been adapted for use in microcultures in flat-bottom 16-mm circular wells of disposable plastic trays. Virus titrations and serum neutralization assays carried out in microcultures yielded reproducible results that were identical to those obtained in macrocultures.
D000818:Animals; D003429:Cross Reactions; D003470:Culture Media; D046508:Culture Techniques; D003587:Cytomegalovirus / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D005347:Fibroblasts; D006041:Goats / Q000276:immunology; D000882:Haplorhini; D006801:Humans; D007106:Immune Sera; D008168:Lung; D008722:Methods; D009500:Neutralization Tests; D014776:Virus Cultivation
D003470:Culture Media; D007106:Immune Sera