An AutoAnalyzer method for estimating serum glyceride glycerol using a glycerokinase procedure.
THE GLYCERIDE GLYCEROL ANALYSIS DEPENDS, AFTER SAPONIFICATION OF TRIGLYCERIDES, ON A LINKED ENZYMATIC PROCEDURE USING GLYCEROKINASE, PYRUVATE KINASE, AND LACTATE DEHYDROGENASE: the final conversion of NADH to NAD(+) is followed fluorimetrically. Twenty analyses can be performed per hour on the AutoAnalyzer; recoveries of added triglycerides ranged between 90 and 104%. In a mixed male and female group the normal range for glyceride glycerol was 2.5 to 15.5 mg/100 ml (0.2-1.4 mmol/l) fasting, and 2.5 to 18.0 mg/100 ml (0.2-1.6 mmol/l) postprandially using fresh serum. There was a significant rise postprandially in older men.
D000328:Adult; D000367:Age Factors; D001322:Autoanalysis; D004435:Eating; D005215:Fasting; D005260:Female; D005470:Fluorometry; D005990:Glycerol / Q000097:blood*; D006801:Humans; D007770:L-Lactate Dehydrogenase; D008297:Male; D008875:Middle Aged; D009243:NAD; D010770:Phosphotransferases; D011770:Pyruvate Kinase; D012737:Sex Factors; D014280:Triglycerides / Q000097:blood*
D014280:Triglycerides; D009243:NAD; D007770:L-Lactate Dehydrogenase; D010770:Phosphotransferases; D011770:Pyruvate Kinase; D005990:Glycerol