Properties of yeast grown anaerobically in media limiting in potassium.
1. Saccharomyces cerevisiae was grown anaerobically in media with different concentrations of K(+) down to less than 1mm. Below 3.2mm the K(+) concentration limited the growth rate and yield. 2. Yeast extract was essential for maximum growth. The yield of cells when the medium contained 0.83mm-K(+) was only 30% of the yield with 90mm-K(+). 3. At the end of anaerobic growth the cells grown in 0.83mm-K(+) had a higher concentration of oxidative enzymes than cells grown in 90mm-K(+). 4. The cells grown anaerobically in 0.83mm-K(+) could adapt to aerobic conditions if K(+) was present in the adaptation medium, but not otherwise. 5. The enzyme pattern of the yeast grown aerobically in 0.83mm-K(+) was very similar to the anaerobically grown cells and did not change markedly after the glucose was consumed.
D000429:Alcohol Oxidoreductases / Q000032:analysis; D000693:Anaerobiosis; D002245:Carbon Dioxide / Q000378:metabolism; D002262:Carboxy-Lyases / Q000032:analysis; D003580:Cytochromes; D003576:Electron Transport Complex IV / Q000032:analysis; D005947:Glucose / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000032:analysis; D009243:NAD; D010088:Oxidoreductases / Q000032:analysis; D010100:Oxygen; D011188:Potassium / Q000378:metabolism*; D011770:Pyruvate Kinase / Q000032:analysis; D012119:Respiration; D012440:Saccharomyces / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism
D003580:Cytochromes; D009243:NAD; D002245:Carbon Dioxide; D012964:Sodium; D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; D008291:Malate Dehydrogenase; D003576:Electron Transport Complex IV; D011770:Pyruvate Kinase; D002262:Carboxy-Lyases; D005947:Glucose; D011188:Potassium; D010100:Oxygen