3',5'-cyclic adenosine monophosphate-requiring mutants of Escherichia coli.
Mutants that require exogenous 3',5'-cyclic adenosine monophosphate (cAMP) for exponential growth were isolated from strains deficient in adenyl cyclase. Studies of one strain showed that cAMP is not incorporated into macromolecules; instead, it seems to have a regulatory function, i.e., in media lacking cAMP, cells form ribonucleic acid (RNA) and protein at linear rather than exponential rates. The exact lesion is not known; ribosomes, messenger RNA, and the beta and beta' subunits of RNA polymerase continue to be made in absence of added cAMP.
D000262:Adenylyl Cyclases; D003090:Coliphages; D000242:Cyclic AMP / Q000378:metabolism*; D004269:DNA, Bacterial; D004586:Electrophoresis; D004926:Escherichia coli / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D005696:Galactosidases / Q000032:analysis; D005815:Genetic Code; D005827:Genetics, Microbial; D007700:Kinetics; D009154:Mutation / Q000187:drug effects*; D009604:Nitrosoguanidines / Q000494:pharmacology; D009693:Nucleic Acid Hybridization; D012316:RNA Nucleotidyltransferases / Q000032:analysis; D012329:RNA, Bacterial / Q000096:biosynthesis; D014161:Transduction, Genetic
D004269:DNA, Bacterial; D009604:Nitrosoguanidines; D012329:RNA, Bacterial; D000242:Cyclic AMP; D012316:RNA Nucleotidyltransferases; D005696:Galactosidases; D000262:Adenylyl Cyclases