title: Preferential transcription of Bacillus subtilis light deoxyribonucleic acid strands during sporulation.
abstract: Messenger ribonucleic acid (RNA) from log and sporulation stages of growth were transcribed mainly from the heavy strand of the complementary strands of Bacillus subtilis deoxyribonucleic acid (DNA). During sporulation a slight transcription shift from heavy to light DNA strands was observed. RNA-DNA hybrid competition experiments revealed that this shift was due to sporulation-specific transcription from light-DNA strands.
mesh_terms: D001412:Bacillus subtilis / Q000254:growth & development* / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D002845:Chromatography; D004269:DNA, Bacterial / Q000302:isolation & purification; D005815:Genetic Code; D005827:Genetics, Microbial; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D011119:Polynucleotides; D012329:RNA, Bacterial; D012333:RNA, Messenger / Q000096:biosynthesis; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide; D013170:Spores / Q000254:growth & development*; D013171:Spores, Bacterial / Q000254:growth & development / Q000378:metabolism; D014316:Tritium; D014529:Uridine / Q000378:metabolism
chemical_list: D002712:Chlorides; D004269:DNA, Bacterial; D011119:Polynucleotides; D012329:RNA, Bacterial; D012333:RNA, Messenger; D014316:Tritium; D002586:Cesium; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide; D014529:Uridine