Action of a phospholipase C preparation on the first component of complement of guinea pig and human serum: lack of correlation with enzyme activity.
Interaction of a phospholipase C (from Clostridium welchii) preparation with the first component of human and guinea pig complement in serum is due to contaminants devoid of phospholipase C activity.
D000818:Animals; D002118:Calcium; D002845:Chromatography; D002850:Chromatography, Gel; D003016:Clostridium perfringens / Q000201:enzymology; D003165:Complement System Proteins; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs / Q000276:immunology; D006801:Humans; D010447:Peptide Hydrolases / Q000032:analysis; D010740:Phospholipases / Q000097:blood / Q000494:pharmacology*; D013696:Temperature
D003165:Complement System Proteins; D010740:Phospholipases; D010447:Peptide Hydrolases; D002118:Calcium