title: Cytopathogenic test for feline leukemia virus infections.
abstract: A mixed culture cytopathogenic effect with the development of multinucleated giant cells in feline leukemia virus-infected cultures after cocultivation with XC cells is described. This cytopathogenic effect has been utilized as a convenient visual marker for the detection and assay of feline leukemia virus in feline and canine embryo fibroblasts as well as in a canine tumor cell line, M-132-1. Comparison of the cytopathogenic reaction in the three cell lines indicates that the reaction is more rapid and pronounced in M-132-1 cells than in the embryo fibroblast cultures.
mesh_terms: D001355:Alpharetrovirus; D000818:Animals; D002415:Cats / Q000196:embryology; D002460:Cell Line; D002478:Cells, Cultured; D003168:Complement Fixation Tests; D003588:Cytopathogenic Effect, Viral; D003601:Cytotoxicity Tests, Immunologic; D004285:Dogs / Q000196:embryology; D005260:Female; D005347:Fibroblasts / Q000382:microbiology; D005354:Fibrosarcoma; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs / Q000276:immunology; D007106:Immune Sera; D008214:Lymphocytes; D008722:Methods; D009374:Neoplasms, Experimental; D009500:Neutralization Tests; D011817:Rabbits / Q000276:immunology; D051381:Rats; D012190:Retroviridae / Q000276:immunology
chemical_list: D007106:Immune Sera