title: Asymmetric template function of microbial deoxyribonucleic acids: transcription of ribosomal and soluble ribonucleic acids.
abstract: In Bacillus subtilis and Escherichia coli, 16 and 23S ribosomal ribonucleic acid (rRNA) hybridize exclusively with the heavy (H) strand of methylated albuminkieselguhr (MAK)-fractionated complementary deoxyribonucleic acid (DNA) strands. All the soluble RNA (4S RNA) in B. subtilis and 66 to 75% of the 4S RNA in E. coli also hybridize with the H strand. Interspecific hybridization shows that E. coli 23S rRNA also binds selectively to the DNA H strand of Salmonella typhimurium. The hybridization peak for all three cellular RNA components is specifically located in the late-eluting region of the absorbance profile of the DNA H strand. The early-eluting region of the light (L) strand preferentially inhibits the hybridization between the peak region of the H strand and 23S rRNA. These regions are considered to represent the transcribing sequences and their complements for 23S rRNA in the separated H and L strands of DNA, respectively.
mesh_terms: D000418:Albumins; D001412:Bacillus subtilis; D002845:Chromatography; D004269:DNA, Bacterial / Q000302:isolation & purification; D004926:Escherichia coli; D005815:Genetic Code; D005827:Genetics, Microbial; D006824:Hybridization, Genetic; D012329:RNA, Bacterial / Q000302:isolation & purification; D012270:Ribosomes; D012486:Salmonella typhimurium; D012822:Silicon Dioxide; D013698:Templates, Genetic; D014316:Tritium; D014498:Uracil
chemical_list: D000418:Albumins; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D014316:Tritium; D014498:Uracil; D012822:Silicon Dioxide