Characterization of messenger-like ribonucleic acid from Saccharomyces cerevisiae by the use of chromatography on methylated albumin-kieselguhr.
Chromatography on methylated albumin-kieselguhr of RNA from Saccharomyces cerevisiae was used to separate stable RNA from a tenaciously bound DNA-like RNA fraction. The tenaciously bound RNA, which was eluted with a dilute solution of sodium dodecyl sulphate, was characterized as messenger-like RNA by its sedimentation behaviour, nucleotide composition, lack of methylated bases and labelling kinetics. Chromatography of purified ribosomal RNA indicated a minor contamination of the tenaciously bound fraction with ribosomal RNA. On the other hand, a large portion of pulse-labelled polyribosomal RNA from protoplasts of Saccharomyces cerevisiae was tenaciously bound to the columns. The ;chase' of isotopic label from the messenger-like RNA was found to be retarded during inhibition of protein synthesis both by cycloheximide and by starvation for a carbon source.
D000418:Albumins; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D002845:Chromatography; D008722:Methods; D008745:Methylation; D008970:Molecular Weight; D009711:Nucleotides / Q000032:analysis; D014176:Protein Biosynthesis; D011523:Protoplasts / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis*; D012270:Ribosomes / Q000032:analysis; D012440:Saccharomyces / Q000032:analysis*; D012822:Silicon Dioxide; D013501:Surface-Active Agents; D014316:Tritium
D000418:Albumins; D002247:Carbon Isotopes; D009711:Nucleotides; D012333:RNA, Messenger; D013501:Surface-Active Agents; D014316:Tritium; D012822:Silicon Dioxide