Immunization of mice against encephalomyocarditis virus. I. Purification, concentration, and inactivation of encephalomyocarditis virus.
Methods are described for the concentration, purification, and inactivation of encephalomyocarditis virus grown in L-cell suspension cultures. Virus concentrates were obtained by the precipitation of crude virus lysates with polyethylene glycol and were purified by Genetron extraction. After column chromatography approximately 70% recovery of infectivity and hemagglutination (HA) activity was found. Exposure of the purified virus suspensions to ultraviolet light resulted in reduction of the infectivity by a factor of 10(9) with little or no loss of HA activity. The preparations are being evaluated as vaccines.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis / Q000528:radiation effects; D002845:Chromatography; D002850:Chromatography, Gel; D004307:Dose-Response Relationship, Radiation; D004686:Encephalomyocarditis virus / Q000187:drug effects / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification; D005557:Formaldehyde / Q000494:pharmacology; D006386:Hemagglutination Tests; D007114:Immunization; D007739:L Cells / Q000276:immunology; D051379:Mice; D011095:Polyethylenes; D011830:Radiation Effects; D014466:Ultraviolet Rays; D014732:Vibration; D010948:Viral Plaque Assay
D000956:Antigens, Viral; D011095:Polyethylenes; D005557:Formaldehyde