Fluorescent modification of adenosine 3',5'-monophosphate: spectroscopic properties and activity in enzyme systems.
The synthesis of a highly fluorescent analog of adenosine 3',5'-monophosphate, namely, 1,N(6)-ethenoadenosine 3',5'-monophosphate, has provided a powerful probe for systems involving adenosine 3',5'-monophosphate. The potential utility of this analog is indicated by its long fluorescent lifetime, detectability at low concentration, and relatively long wavelength of excitation (300 nanometers). In protein kinase systems it is a highly acceptable substitute for adenosine 3',5'-monophosphate.
D000079:Acetaldehyde; D000080:Acetals; D000255:Adenosine Triphosphate; D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002713:Chlorine; D002855:Chromatography, Thin Layer; D000242:Cyclic AMP / Q000138:chemical synthesis / Q000031:analogs & derivatives; D004789:Enzyme Activation; D005453:Fluorescence; D007093:Imidazoles / Q000138:chemical synthesis; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D010727:Phosphoric Diester Hydrolases; D010759:Phosphorus Isotopes; D010770:Phosphotransferases; D011485:Protein Binding; D011506:Proteins; D011817:Rabbits; D013053:Spectrophotometry; D013329:Structure-Activity Relationship; D014316:Tritium; D014466:Ultraviolet Rays
D000080:Acetals; D007093:Imidazoles; D010759:Phosphorus Isotopes; D011506:Proteins; D014316:Tritium; C001626:1,N(6)-ethenoadenosine 3',5'-monophosphate; D002713:Chlorine; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D010770:Phosphotransferases; D010727:Phosphoric Diester Hydrolases; D000079:Acetaldehyde