1,25-dihydroxycholecalciferol: metabolite of vitamin D3 active on bone in anephric rats.
Nephrectomy prevents completely the bone calcium mobilization response to 25-hydroxycholecalciferol. In contrast it does not prevent this response to 1,25-dihydroxycholecalciferol. Because it is known that the kidney is the site of 1,25-dihydroxycholecalciferol formation, these results provide evidence that 1,25-dihydroxycholecalciferol or a further metabolite thereof and not 25-hydroxycholecalciferol is the metabolically active form of vitamin D(3) responsible for bone calcium mobilization.
D000818:Animals; D001345:Autoradiography; D001842:Bone and Bones / Q000378:metabolism*; D002118:Calcium / Q000097:blood / Q000378:metabolism*; D002127:Calcium Isotopes; D002762:Cholecalciferol / Q000378:metabolism*; D006887:Hydroxycholecalciferols / Q000378:metabolism / Q000494:pharmacology; D007668:Kidney / Q000378:metabolism*; D008026:Ligation; D008297:Male; D009392:Nephrectomy; D051381:Rats; D014513:Ureter / Q000601:surgery
D002127:Calcium Isotopes; D006887:Hydroxycholecalciferols; D002762:Cholecalciferol; D002118:Calcium