Neutralization of sensitized virus by purified components of complement.
Herpes simplex virus which had been sensitized with immunoglobulin M antibody was neutralized by serum deficient in the fifth and sixth components of complement (C) but not by serum deficient in the fourth component C (C4). The sequential addition of the functionally purified components of C showed that the activated first component of C (C1[unk]) failed to neutralize sensitized virus. However, in the presence of an optimal concentration of C1[unk], the addition of C4 resulted in neutralization. The amount of virus neutralized was dependent upon the concentration of immunoglobulin M used to sensitize the virus and the concentration of C1[unk] and C4. The addition of the second component of C (C2) to reaction mixtures containing an optimal concentration of C1[unk] and a limiting concentration of C4 resulted in increased neutralization and the amount of virus neutralized was dependent upon the concentration of C2. The addition of the third component of C (C3) to reaction mixtures containing an optimal concentration of C1[unk] and limiting concentrations of C4 and C2 also resulted in increased neutralization and the amount of virus neutralized was dependent upon the concentration of C3.
D000818:Animals; D002460:Cell Line; D003165:Complement System Proteins / Q000494:pharmacology*; D006168:Guinea Pigs; D007106:Immune Sera / Q000494:pharmacology*; D007136:Immunoglobulins / Q000494:pharmacology; D007668:Kidney; D007700:Kinetics; D051379:Mice; D009500:Neutralization Tests; D011817:Rabbits; D018139:Simplexvirus / Q000187:drug effects / Q000276:immunology*
D007106:Immune Sera; D007136:Immunoglobulins; D003165:Complement System Proteins