title: Mutants of Salmonella typhimurium and Escherichia coli pleiotropically defective in active transport.
abstract: Two classes of mutants isolated from E. coli and Salmonella typhimurium are altered in respiration-coupled active transport, as studied in whole cells and/or isolated membrane vesicles. Mutant cells defective in D-lactate dehydrogenase (dld) transport amino acids and lactose normally. Membrane vesicles prepared from these mutants do not exhibit D-lactate-dependent transport, D-lactate oxidation, or D-lactate: dichlorophenolindophenol reductase activity. However, succinate-dependent transport is markedly enhanced in these mutants, without a corresponding increase in succinic dehydrogenase activity. The second class of mutants is defective in the coupling of electron transfer to active transport. Whole cells and membrane vesicles prepared from these etc mutants exhibit markedly reduced ability to transport amino acids, despite the ability of the vesicles to oxidize D-lactate, succinate, and NADH. Vectorial phosphorylation of alpha-methylglucoside by these mutants is normal. Electrontransfer coupling mutants are similar phenotypically to mutants uncoupled for oxidative phosphorylation (uncA), but have normal ATPase activity. Moreover, uncA mutants catalyze active transport as well as does the wild type. These experiments indicate that the ETC component is essential for the coupling of respiratory energy to active transport, and provide further evidence that the generation or utilization of ATP is not involved in these transport mechanisms.
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D001693:Biological Transport, Active; D002462:Cell Membrane / Q000378:metabolism; D002874:Chromosome Mapping; D004579:Electron Transport; D004926:Escherichia coli / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D007773:Lactates / Q000378:metabolism*; D007785:Lactose / Q000378:metabolism; D009154:Mutation; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D010085:Oxidative Phosphorylation; D012486:Salmonella typhimurium / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D013104:Spheroplasts / Q000378:metabolism; D013386:Succinates / Q000378:metabolism; D014161:Transduction, Genetic
chemical_list: D000596:Amino Acids; D007773:Lactates; D013386:Succinates; D009243:NAD; D007770:L-Lactate Dehydrogenase; D000251:Adenosine Triphosphatases; D007785:Lactose