title: Deoxyribonucleic acid polymerases of Rous sarcoma virus: kinetics of deoxyribonucleic acid synthesis and specificity of the products.
abstract: The deoxyribonucleic acid (DNA) polymerase(s) of Rous sarcoma virus synthesizes two principal products-single-stranded DNA in the form of a DNA:ribonucleic acid (RNA) hybrid and double-stranded DNA. All of the single-stranded product and 50% of the double-stranded product can be hybridized to 70S viral RNA. These results, in combination with data obtained by analysis of the kinetics of double-stranded DNA synthesis, indicate that the synthesis of double-stranded DNA is a sequel to the synthesis of single-stranded DNA and is dependent upon the latter for the provision of initial template.
mesh_terms: D000818:Animals; D001358:Avian Sarcoma Viruses / Q000032:analysis / Q000201:enzymology* / Q000302:isolation & purification; D002499:Centrifugation, Density Gradient; D002501:Centrifugation, Zonal; D002586:Cesium; D002642:Chick Embryo; D046508:Culture Techniques; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D005347:Fibroblasts; D005827:Genetics, Microbial; D006882:Hydroxyapatites; D009693:Nucleic Acid Hybridization; D010636:Phenols; D012367:RNA, Viral / Q000302:isolation & purification; D012964:Sodium; D013395:Sucrose; D013431:Sulfates; D013698:Templates, Genetic; D013942:Thymine Nucleotides / Q000378:metabolism; D014316:Tritium; D014776:Virus Cultivation
chemical_list: D004279:DNA, Viral; D006882:Hydroxyapatites; D010636:Phenols; D012367:RNA, Viral; D013431:Sulfates; D013942:Thymine Nucleotides; D014316:Tritium; D002586:Cesium; D013395:Sucrose; D012964:Sodium; D004254:DNA Nucleotidyltransferases