A method for the rapid isolation of nuclear membranes from rat liver. Characterisation of the membrane preparation and its associated DNA polymerase.
D000818:Animals; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000201:enzymology*; D002850:Chromatography, Gel; D004247:DNA / Q000032:analysis; D004254:DNA Nucleotidyltransferases / Q000302:isolation & purification*; D004261:DNA Replication; D003851:Deoxyribonucleases; D005260:Female; D005952:Glucose-6-Phosphatase / Q000032:analysis; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D008297:Male; D008566:Membranes / Q000032:analysis / Q000201:enzymology*; D008722:Methods; D051379:Mice; D008853:Microscopy; D008854:Microscopy, Electron; D008861:Microsomes / Q000201:enzymology; D009698:Nucleoproteins / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D012313:RNA / Q000032:analysis; D051381:Rats; D012965:Sodium Chloride; D013942:Thymine Nucleotides
D009698:Nucleoproteins; D010743:Phospholipids; D013942:Thymine Nucleotides; D012965:Sodium Chloride; D012313:RNA; D004247:DNA; D004254:DNA Nucleotidyltransferases; D003851:Deoxyribonucleases; D005952:Glucose-6-Phosphatase