Absence of interference during high-multiplicity infection by clonally purified vesicular stomatitis virus.
Stocks of vesicular stomatitis virus free of defective interfering particles were produced by serial clonal isolation. High-multiplicity infections with these stocks led to no interference or formation of defective interfering particles. Defective interfering particles were generated by three successive passages at high multiplicity.
D000362:Agar; D000818:Animals; D002499:Centrifugation, Density Gradient; D002999:Clone Cells; D006224:Cricetinae; D046508:Culture Techniques; D003609:Dactinomycin; D005260:Female; D010053:Ovary; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis; D013395:Sucrose; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D014721:Vesicular stomatitis Indiana virus / Q000032:analysis / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism / Q000472:pathogenicity; D014762:Viral Interference; D014776:Virus Cultivation; D014779:Virus Replication
D012367:RNA, Viral; D014316:Tritium; D003609:Dactinomycin; D013395:Sucrose; D000362:Agar; D014529:Uridine