Biosynthesis of presqualene pyrophosphate by liver microsomes.
Microsomes from rat liver have been shown to synthesize a squalene precursor from farnesyl pyrophosphate. This intermediate is identical with presqualene pyrophosphate, a 30-carbon cyclopropane containing pyrophosphate ester that had previously been isolated from yeast. The squalene precursor was found to be tightly, but not covalently, bound to microsomes.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002247:Carbon Isotopes; D002498:Centrifugation; D002845:Chromatography; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D011756:Diphosphates / Q000096:biosynthesis* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008861:Microsomes / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats; D013185:Squalene / Q000096:biosynthesis*; D014316:Tritium; D015003:Yeasts / Q000378:metabolism
D002247:Carbon Isotopes; D011756:Diphosphates; D014316:Tritium; D013185:Squalene; D000469:Alkaline Phosphatase