Competing addition and hydrolysis of the cytidylylcytidylyladenosine terminal residues of transfer ribonucleic acid isolated from the non-lactating bovine mammary gland.
1. The enzyme fraction obtained from the pH5 enzyme of non-lactating bovine mammary gland between 40 and 100% ammonium sulphate saturation markedly inhibited the AMP-incorporating activity of rat liver nucleotide-incorporating enzyme. This inhibitory effect has been attributed to high nuclease activity which can be partially removed by adsorption of the enzyme fraction on to calcium phosphate gel. 2. The degradation action of the calcium phosphate-purified enzyme is confined mainly to the terminal trinucleotide sequence -pCpCpA of tRNA, its effect being analogous to that of venom phosphodiesterase. This enzyme is heat labile and very readily loses its degradative activity. 3. Treatment of the enzyme fraction with Macaloid results in complete removal of the phosphodiesterase, leaving an enzyme capable of incorporating AMP into tRNA. 4. Transfer RNA extracted from non-lactating bovine mammary gland in the presence of polyvinyl sulphate and Macaloid is able to accept amino acids with an efficiency 30% of that shown by lactating bovine mammary-gland tRNA isolated under identical conditions.
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D002130:Calcium Phosphates; D002247:Carbon Isotopes; D002417:Cattle; D003597:Cytosine Nucleotides / Q000378:metabolism; D004355:Drug Stability; D005260:Female; D006358:Hot Temperature; D066298:In Vitro Techniques; D007774:Lactation; D008321:Mammary Glands, Animal / Q000201:enzymology / Q000378:metabolism*; D009711:Nucleotides / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases; D011247:Pregnancy; D012343:RNA, Transfer / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D012260:Ribonucleases / Q000378:metabolism; D014316:Tritium; D014688:Venoms
D000227:Adenine Nucleotides; D002130:Calcium Phosphates; D002247:Carbon Isotopes; D003597:Cytosine Nucleotides; D009711:Nucleotides; D014688:Venoms; D014316:Tritium; D012343:RNA, Transfer; D012260:Ribonucleases; D010744:Phosphoric Monoester Hydrolases