Electron transport systems of Nitrosomonas: isolation of a membrane-envelope fraction.
Freezing and thawing of Nitrosomonas, followed by centrifugation of the homogenate at 3,000 x g, resulted in a fraction which appeared to consist of an intact membrane-envelope complex and contained approximately 50% of the cell protein and more than 90% of the ubiquinone and cytochrome A-type mammalian cytochrome c oxidase activity. The supernatant fraction, resulting from subsequent centrifugation of the extract at 100,000 x g, contained approximately 50% of the cell protein and more than 80% of the B- and C-type cytochrome and P-463 and the enzymes glutamate dehydrogenase; hydroxylamine dehydrogenase; nitrite synthetase; nitrite reductase; and 2,6-dichlorophenolindophenol-, p-phenylenediamine-, pyrogallol-, and hydroquinone-oxidase. Data on the concentration of electron transport components in Nitrosomonas are presented.
D001426:Bacterial Proteins / Q000032:analysis; D002462:Cell Membrane / Q000032:analysis / Q000201:enzymology; D002845:Chromatography; D002855:Chromatography, Thin Layer; D003580:Cytochromes / Q000032:analysis; D004579:Electron Transport; D003576:Electron Transport Complex IV / Q000032:analysis; D005969:Glutamate Dehydrogenase / Q000032:analysis; D006898:Hydroxylamines; D008854:Microscopy, Electron; D009573:Nitrites; D009606:Nitrosomonas / Q000032:analysis / Q000166:cytology / Q000201:enzymology; D010088:Oxidoreductases / Q000032:analysis; D013053:Spectrophotometry; D013347:Subcellular Fractions / Q000032:analysis / Q000201:enzymology; D014451:Ubiquinone / Q000032:analysis / Q000302:isolation & purification; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D003580:Cytochromes; D006898:Hydroxylamines; D009573:Nitrites; D014451:Ubiquinone; D010088:Oxidoreductases; D005969:Glutamate Dehydrogenase; D003576:Electron Transport Complex IV