Specificity of a catabolic pathway--a lesson learned from indirect assays.
Studies with purified orcinol hydroxylase suggest that, contrary to previous conclusions, the enzymes of the orcinol pathway cannot transform analogous compounds to common metabolites. The substrate analogues of orcinol uncouple electron flow from reduced nicotinamide adenine dinucleotide to oxygen from the hydroxylation reaction catalyzed by orcinol hydroxylase.
D002474:Cell-Free System; D003408:Cresols / Q000378:metabolism; D003470:Culture Media; D004579:Electron Transport; D006861:Hydrogen Peroxide / Q000096:biosynthesis; D006899:Mixed Function Oxygenases / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D010636:Phenols / Q000378:metabolism; D011048:Polarography; D011549:Pseudomonas / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D012118:Resorcinols / Q000378:metabolism
D003408:Cresols; D003470:Culture Media; D010636:Phenols; D012118:Resorcinols; D009243:NAD; D006861:Hydrogen Peroxide; D006899:Mixed Function Oxygenases