title: Direct evidence for a prostaglandin receptor and its application to prostaglandin measurements (rat-adipocytes-antagonists-analogues-mouse ovary assay).
abstract: A prostaglandin receptor is present in rat adipocytes, as shown by competition studies with [(3)H]-prostaglandin E(1). The affinity of the E prostaglandins for this receptor preparation is greater than that of A and F prostaglandins, an observation consistent with their relative potencies in stimulating cyclic AMP formation in isolated mouse ovaries and various other organs. In the protocol described, binding constants of 3-8 nM were obtained with prostaglandins E(2) and E(1). A sensitivity of 1 pmole makes this method readily applicable for measurement of tissue concentrations of the E prostaglandins.
mesh_terms: D000273:Adipose Tissue / Q000032:analysis* / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D000242:Cyclic AMP / Q000378:metabolism; D005260:Female; D008055:Lipids / Q000494:pharmacology; D008297:Male; D008722:Methods; D051379:Mice; D008832:Microchemistry; D010053:Ovary / Q000187:drug effects / Q000378:metabolism; D011453:Prostaglandins / Q000032:analysis* / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D011955:Receptors, Drug; D013268:Stimulation, Chemical; D014316:Tritium
chemical_list: D008055:Lipids; D011453:Prostaglandins; D011955:Receptors, Drug; D014316:Tritium; D000242:Cyclic AMP