RNA-dependent RNA polymerase activity associated with virions and subviral particles of myxoviruses.
In vitro RNA synthesis is catalyzed by an enzyme found in purified virions of each of nine influenza strains tested. The extent of [(3)H]UTP incorporation observed is strain dependent, with an obligate requirement for Mn(2+) ions and RNA template. At least 30% of purified product RNA shows resistance to degradation by ribonuclease A and T1 before and after annealing with exogenous RNA from influenza virions.
D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D066298:In Vitro Techniques; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D009522:Newcastle disease virus / Q000201:enzymology*; D009975:Orthomyxoviridae / Q000201:enzymology*; D012316:RNA Nucleotidyltransferases / Q000032:analysis; D012367:RNA, Viral / Q000096:biosynthesis; D012260:Ribonucleases / Q000494:pharmacology; D013045:Species Specificity; D013698:Templates, Genetic; D014316:Tritium; D014500:Uracil Nucleotides / Q000378:metabolism; D014721:Vesicular stomatitis Indiana virus / Q000201:enzymology
D012367:RNA, Viral; D014500:Uracil Nucleotides; D014316:Tritium; D008345:Manganese; D012316:RNA Nucleotidyltransferases; D012260:Ribonucleases; D008274:Magnesium