Specific assay of aminoglycosidic- or polymyxin-type antibiotics present in human sera in combination with cephalosporins.
Monitoring of serum concentrations of aminoglycosidic or polymyxin antibiotics when administered concurrently with cephalosporins or penicillins requires a special assay technique. Selective enzymatic degradation of the beta-lactam antibiotic from the serum specimen allows subsequent assay of the antibiotic being monitored. This report gives details of a simple procedure for laboratory production of a crude enzyme capable of degrading cephalosporins or penicillins. An assay procedure for quantitating aminoglycosidic or polymyxin antibiotics after enzymatic degradation of a coexisting beta-lactam antibiotic is described.
D000581:Amidohydrolases / Q000302:isolation & purification / Q000378:metabolism; D000606:Amino Sugars / Q000097:blood; D000900:Anti-Bacterial Agents / Q000097:blood*; D001412:Bacillus subtilis; D001681:Biological Assay; D001884:Bordetella; D002511:Cephalosporins / Q000097:blood* / Q000378:metabolism / Q000627:therapeutic use; D003091:Colistin / Q000097:blood* / Q000627:therapeutic use; D004357:Drug Synergism; D004754:Enterobacter / Q000201:enzymology; D005839:Gentamicins / Q000097:blood* / Q000627:therapeutic use; D006027:Glycosides / Q000097:blood; D006801:Humans; D007612:Kanamycin / Q000097:blood* / Q000627:therapeutic use; D008722:Methods; D011113:Polymyxins / Q000097:blood* / Q000627:therapeutic use; D012505:Sarcina; D013053:Spectrophotometry; D013307:Streptomycin / Q000097:blood* / Q000627:therapeutic use
D000606:Amino Sugars; D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D005839:Gentamicins; D006027:Glycosides; D011113:Polymyxins; D007612:Kanamycin; D000581:Amidohydrolases; D013307:Streptomycin; D003091:Colistin