Complete transcription by the transcriptase of vesicular stomatitis virus.
Vesicular stomatitis virus (VSV) transcriptase completely transcribes the VSV genome into messenger-size ribonucleic acid (RNA) species. The replicating complexes of template and product RNA, generated in vitro by VSV transcriptase, exhibit a mass parity between product and template molecules. Purified product RNA, after annealing to small amounts of viral RNA, rendered 92% of the viral RNA ribonuclease-resistant. It is calculated that, under optimal conditions, 60 min is required before the VSV genome is completely transcribed.
D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002852:Chromatography, Ion Exchange; D006224:Cricetinae; D004589:Electrophoresis, Disc; D005815:Genetic Code; D005827:Genetics, Microbial; D007668:Kidney; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010759:Phosphorus Isotopes; D012316:RNA Nucleotidyltransferases / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D012367:RNA, Viral / Q000302:isolation & purification; D012260:Ribonucleases; D013395:Sucrose; D013698:Templates, Genetic; D013997:Time Factors; D014316:Tritium; D014500:Uracil Nucleotides / Q000378:metabolism; D014529:Uridine / Q000378:metabolism; D014721:Vesicular stomatitis Indiana virus / Q000201:enzymology* / Q000302:isolation & purification / Q000378:metabolism; D014776:Virus Cultivation
D010759:Phosphorus Isotopes; D012333:RNA, Messenger; D012367:RNA, Viral; D014500:Uracil Nucleotides; D014316:Tritium; D013395:Sucrose; D012316:RNA Nucleotidyltransferases; D012260:Ribonucleases; D014529:Uridine