Improvements in the growth of BHK-21 cells in submerged culture.
A medium is described which will support the submerged culture of BHK-21 cells to 6.5 x 10(6) to 7 x 10(6) cells/ml in volumes up to 30 liters. Shaken-flask cultures were used to determine nutrient deficiencies in depleted medium. The final medium became limited by glutamine at 6.5 x 10(6) to 7 x 10(6) cells/ml, but increasing the glutamine concentration failed to improve cell yields. The value of the polyol Pluronic F68 as a protective substance is illustrated.
D000418:Albumins; D000596:Amino Acids; D000693:Anaerobiosis; D000818:Animals; D005537:Aphthovirus; D001428:Bacterial Vaccines; D002245:Carbon Dioxide; D002452:Cell Count; D002460:Cell Line / Q000187:drug effects / Q000254:growth & development*; D006224:Cricetinae; D003470:Culture Media / Q000592:standards*; D005947:Glucose; D005973:Glutamine / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007668:Kidney; D011108:Polymers / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology; D013696:Temperature; D014815:Vitamins
D000418:Albumins; D000596:Amino Acids; D001428:Bacterial Vaccines; D003470:Culture Media; D011108:Polymers; D013501:Surface-Active Agents; D014815:Vitamins; D005973:Glutamine; D002245:Carbon Dioxide; D005947:Glucose