Selective inhibition of nuclear DNA synthesis by 9- -D-arabinofuranosyl adenine in rat cells transformed by Rous sarcoma virus.
The drug, 9-beta-D-arabinofuranosyl adenine, selectively inhibits the synthesis of nuclear DNA without affecting extrachromosomal DNA synthesis in rat cells transformed by Rous sarcoma virus (B-mix K-44/6). The inhibition was linear with respect to drug concentration over the range of 37-600 muM. Mitosis and total synthesis of DNA per cell were also depressed. DNA synthesis was determined by measurement of [(3)H]thymidine incorporation into DNA. Covalently-closed circular DNA was extracted by the Hirt procedure and separated from residual chromosomal DNA by buoyant density gradient ultracentrifugation in CsCl-propidium diiodide. On the basis of buoyant density and sedimentation velocity centrifugation, the covalently-closed circular DNA formed in the presence of the drug was indistinguishable from that formed in its absence.
D000225:Adenine / Q000494:pharmacology; D000818:Animals; D001089:Arabinose / Q000494:pharmacology; D001358:Avian Sarcoma Viruses; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects* / Q000378:metabolism; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000096:biosynthesis*; D004622:Embryo, Mammalian / Q000187:drug effects / Q000378:metabolism; D007454:Iodides; D008938:Mitosis / Q000187:drug effects; D009705:Nucleosides / Q000494:pharmacology*; D010617:Phenanthridines; D051381:Rats; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D007454:Iodides; D009705:Nucleosides; D010617:Phenanthridines; D014316:Tritium; D004247:DNA; D001089:Arabinose; D000225:Adenine; D013936:Thymidine