Formation of hydrogen peroxide by group N streptococci and its effect on their growth and metabolism.
The formation of hydrogen peroxide by group N streptococci was found to occur through the action of a reduced nicotinamide adenine dinucleotide (NADH) oxidase which catalyzed the oxidation of NADH by molecular oxygen. The enzyme was activated by flavine adenine dinucleotide. Whereas some of the hydrogen peroxide formed was removed through the action of an NADH peroxidase, sufficient accumulated in media to inhibit the growth, respiration, and viability of these organisms. The amount of hydrogen peroxide which accumulated varied among strains, and this variation could be related to differences in the properties of the NADH oxidase present.
D002374:Catalase / Q000378:metabolism; D002474:Cell-Free System; D003470:Culture Media; D003864:Depression, Chemical; D004789:Enzyme Activation; D005182:Flavin-Adenine Dinucleotide / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000096:biosynthesis* / Q000494:pharmacology; D008365:Manometry; D009243:NAD / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D010101:Oxygen Consumption; D010544:Peroxidases / Q000378:metabolism; D013053:Spectrophotometry; D013291:Streptococcus / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*
D003470:Culture Media; D009243:NAD; D005182:Flavin-Adenine Dinucleotide; D006861:Hydrogen Peroxide; D010088:Oxidoreductases; D010544:Peroxidases; D002374:Catalase; D005947:Glucose