The regulation of xanthine oxidase. Inhibition by reduced nicotinamide-adenine dinucleotide of rat liver xanthine oxidase type D and of chick liver xanthine dehydrogenase.
1. Rat liver xanthine oxidase type D (NAD(+)-dependent) and chick liver xanthine oxidase are inhibited by NADH, which competes with NAD(+). 2. The addition of a NADH-reoxidizing system in the assay of these enzyme activities is proposed. 3. Rat liver xanthine oxidase type O (oxygen-dependent) is not affected by NADH.
D000818:Animals; D001681:Biological Assay; D002645:Chickens; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D009243:NAD / Q000494:pharmacology*; D010100:Oxygen; D051381:Rats; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors*
D009243:NAD; D014969:Xanthine Oxidase; D010100:Oxygen