An adhesive surface sampling technique for airborne viruses.
A mixture of sucrose, glycerol and bovine serum albumin produces a stable coating in a Petri dish which remains adhesive for up to an hour when exposed in a slit sampler. Virus aerosols can be collected on this surface followed by the direct addition of cell cultures to demonstrate the presence of viable virus. The technique is applicable to the Andersen sampler. A modified version of this sampler has been produced with the same particle collection efficiency as the standard Andersen sampler. The plaque counts obtained by the adhesive surface sampling technique are believed to give an indication of the number of particles collected bearing viable virus.
D000391:Air Microbiology; D000818:Animals; D001412:Bacillus subtilis / Q000302:isolation & purification; D002642:Chick Embryo; D003470:Culture Media; D046508:Culture Techniques; D005990:Glycerol; D006168:Guinea Pigs; D006801:Humans; D017955:Poliovirus / Q000302:isolation & purification; D012672:Semliki forest virus / Q000302:isolation & purification; D012710:Serum Albumin, Bovine; D013395:Sucrose; D013499:Surface Properties; D014616:Vaccinia virus / Q000302:isolation & purification; D014780:Viruses / Q000302:isolation & purification*
D003470:Culture Media; D012710:Serum Albumin, Bovine; D013395:Sucrose; D005990:Glycerol