Rabbit muscle gangliosides.
Four ganglioside fractions were isolated from rabbit muscle: one hematoside and three hexosamine-containing species. They were analyzed for hexoses, hexosamine, sialic acid, fatty acids, and long-chain base content. The molar ratios of sphingosine-hexose-hexosamine-sialic acid were: for hematoside, 1:2:0:1; for the disialogangliosides, 1:3:1:2; and for trisialoganglioside, 1:3:1:3. The carbohydrates were studied by thin-layer and paper chromatography. The hexoses were glucose and galactose; the hexosamine was N-acetylgalactosamine and the sialic acid was N-acetylneuraminic acid. Fatty acids and long-chain bases were analyzed by gas-liquid chromatography. The fatty acid composition was similar in all of the four gangliosides. The most abundant fatty acids were 16:0 and 18:0, but significant amounts of 16:1, 18:1, 20:0, and 22:0 were also found. Hydroxy fatty acids were not detected. In all of the muscle gangliosides the main long-chain bases were C(18)-sphingenine and C(20)-sphingenine. In hematoside there were also measurable amounts of C(18)-sphinganine and C(20)-sphinganine, whereas in the major gangliosides only traces of C(18)-sphinganine were detected.
D000818:Animals; D002852:Chromatography, Ion Exchange; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D003016:Clostridium perfringens / Q000201:enzymology; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D005690:Galactose / Q000032:analysis; D005732:Gangliosides / Q000032:analysis / Q000302:isolation & purification*; D005947:Glucose / Q000032:analysis; D006595:Hexosamines / Q000032:analysis; D006601:Hexoses / Q000302:isolation & purification; D009132:Muscles / Q000032:analysis*; D009438:Neuraminic Acids / Q000032:analysis; D009439:Neuraminidase; D011817:Rabbits; D013110:Sphingosine / Q000032:analysis
D005227:Fatty Acids; D005732:Gangliosides; D006595:Hexosamines; D006601:Hexoses; D009438:Neuraminic Acids; D009439:Neuraminidase; D005947:Glucose; D013110:Sphingosine; D005690:Galactose