Increased uptake of amino acids and 2-deoxy-D-glucose by virus-transformed cells in culture.
Transformed and nontransformed cells in tissue culture differ in their rate of uptake of certain nutrients, as determined by a polyester-coverslip technique. A 2.5- to 3.5-fold increased rate of uptake of alpha-aminoisobutyric acid, cycloleucine, and 2-deoxy-D-glucose was observed with polyoma virus-transformed baby hamster kidney (BHK) 21 cells and simian virus 40 (SV40)-transformed BALB/3T3 (mouse fibroblast) cells, compared to their nontransformed counterparts. Kinetic analysis suggested that the increased uptake by cells transformed with virus was associated with a 3-fold greater V(max), with no detectable changes in apparent K(m). Limited studies also revealed increased initial rates of uptake by murine sarcoma virus-transformed rat liver cells, as compared to the parental line. Exposure of cells to concanavalin A and wheat-germ agglutinin led to significant reductions in amino-acid uptake by both transformed and nontransformed cells; however, transformed cells showed a greater decrease in uptake after exposure to wheat-germ agglutinin. Increased initial rates of uptake of certain amino acids and sugars may be a feature common to transformed cells, compared to their parental control.
D000596:Amino Acids / Q000378:metabolism*; D000621:Aminoisobutyric Acids / Q000378:metabolism; D000818:Animals; D001120:Arginine / Q000378:metabolism; D002247:Carbon Isotopes; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D006224:Cricetinae; D006601:Hexoses / Q000378:metabolism*; D007700:Kinetics; D037102:Lectins / Q000494:pharmacology; D007930:Leucine / Q000378:metabolism; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism* / Q000382:microbiology; D011120:Polyomavirus; D051381:Rats; D013539:Simian virus 40
D000596:Amino Acids; D000621:Aminoisobutyric Acids; D002247:Carbon Isotopes; D006601:Hexoses; D037102:Lectins; D003208:Concanavalin A; D001120:Arginine; D007930:Leucine