Formate dehydrogenase from Clostridium acidiurici.
Partial purification of formate dehydrogenase from Clostridium acidiurici has been accomplished, and some properties of the enzyme have been determined. The molecular weight of the protein is at least 200,000 daltons. The enzyme showed marked instability to freezing and thawing and was inhibited strongly by oxygen and by light. Such inhibition was not reversed by incubation in the presence of thiol compounds. Cyanide inhibited the enzyme 90% at 0.1 mm concentrations, but ethylenediaminetetraacetate produced only slight inhibition at concentrations as high as 50 mm. The purified enzyme showed no ferredoxin activity in the Clostridium pasteurianum clastic system during pyruvate oxidation. Crude preparations of the enzyme could be coupled through ferredoxin to the reduction of nicotinamide adenine dinucleotide during formate oxidation, but the purified enzyme could not catalyze the reduction of pyridine nucleotides by formate in the presence of ferredoxin. Formate oxidation with the purified enzyme was readily coupled to benzyl viologen reduction, in which case ferredoxin was not required. An exchange between formate and bicarbonate was catalyzed by both crude and purified preparations of the enzyme, but the net synthesis of formate from CO(2) was not accomplished.
D000645:Ammonium Sulfate; D001593:Benzyl Compounds / Q000378:metabolism; D001639:Bicarbonates / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D002247:Carbon Isotopes; D002474:Cell-Free System; D011232:Chemical Precipitation; D002852:Chromatography, Ion Exchange; D003013:Clostridium / Q000201:enzymology* / Q000254:growth & development; D003470:Culture Media; D003486:Cyanides / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004579:Electron Transport; D005288:Ferredoxins / Q000378:metabolism; D005561:Formates / Q000378:metabolism; D005615:Freezing; D008027:Light; D008970:Molecular Weight; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism; D010100:Oxygen; D011479:Protamines; D011773:Pyruvates / Q000378:metabolism; D013053:Spectrophotometry; D013439:Sulfhydryl Reagents / Q000494:pharmacology
D001593:Benzyl Compounds; D001639:Bicarbonates; D002247:Carbon Isotopes; D003470:Culture Media; D003486:Cyanides; D005288:Ferredoxins; D005561:Formates; D011479:Protamines; D011773:Pyruvates; D013439:Sulfhydryl Reagents; D009243:NAD; D002245:Carbon Dioxide; D004492:Edetic Acid; D010088:Oxidoreductases; D010100:Oxygen; D000645:Ammonium Sulfate