Preparative electrophoresis of isotopically labeled L-cell interferons.
The preparative method of polyacrylamide gel electrophoresis was adapted for purification and characterization of isotopically labeled L-cell interferons. Re-covery of interferon activity was quantitative, and purification and resolution were comparable to those obtained by analytical polyacrylamide gel electrophoresis. Ultimate specific activities attainable ranged from 2 x 10(6) to 3 x 10(6) international units per mg of protein.
D001681:Biological Assay; D002852:Chromatography, Ion Exchange; D004589:Electrophoresis, Disc; D005374:Filtration; D007372:Interferons / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification*; D007525:Isoelectric Focusing; D007739:L Cells / Q000378:metabolism; D008722:Methods; D009522:Newcastle disease virus; D011070:Poly I-C; D011492:Protein Hydrolysates; D014316:Tritium; D014721:Vesicular stomatitis Indiana virus
D011492:Protein Hydrolysates; D014316:Tritium; D007372:Interferons; D011070:Poly I-C