title: Mitochondrial longevity in vitro: the retention of respiratory control.
abstract: Isolated mitochondria were maintained metabolically active and coupled, i.e., capable of respiratory control, for several hours at 25 degrees C. An increase in respiratory control generally occurred during the first 4-8 hr of incubation followed thereafter by a decline. Longevity is a dynamic function as evidenced by its dependency on substrate and cofactors, thiamine pyrophosphate, in particular. Magnesium was also essential to longevity; coenzyme A, bovine serum albumin, and reducing agents were not. The findings are discussed in terms of cytoplasmic-mitochondrial interrelationships and mitochondrial autonomy.
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism*; D002701:Chloramphenicol / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D003593:Cytoplasm; D005638:Fruit; D066298:In Vitro Techniques; D008274:Magnesium / Q000494:pharmacology; D008928:Mitochondria / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D010101:Oxygen Consumption; D010945:Plants, Edible / Q000378:metabolism*; D011048:Polarography; D013835:Thiamine Pyrophosphate / Q000378:metabolism; D013997:Time Factors
chemical_list: D000227:Adenine Nucleotides; D009243:NAD; D002701:Chloramphenicol; D003513:Cycloheximide; D008274:Magnesium; D013835:Thiamine Pyrophosphate