Characterization of the strain of adenovirus type 7 carrying the defective monkey cell-adapting component.
The strain of adenovirus type 7 carrying the defective monkey cell-adapting component (MAC) has been further characterized. MAC is more sensitive to inactivation by ultraviolet light than the associated adenovirus, which, in turn, is more rapidly inactivated than complete simian virus 40 (SV40). The 37% dose was 16 sec for MAC, 60 sec for adenovirus, and 84 sec for SV40. Filtration through membranes revealed that both MAC and adenovirus were retained by 100-mmu filters. MAC equilibrated in cesium chloride at the same buoyant density as the complete adenovirions (1.34 g/ml). Deoxyribonucleic acid extracted from green monkey kidney cells infected with MAC-adenovirus 7 did not hybridize in vitro with SV40 complementary ribonucleic acid. The two components (MAC and adenovirus) of the virus population possess such similar biophysical properties that their separation has not yet been achieved. Evidence to date indicates that MAC is probably not derived from SV40.
D000256:Adenoviridae / Q000145:classification* / Q000528:radiation effects; D000818:Animals; D002499:Centrifugation, Density Gradient; D046508:Culture Techniques; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000378:metabolism; D003673:Defective Viruses; D000882:Haplorhini; D006378:Helper Viruses; D006801:Humans; D006824:Hybridization, Genetic; D007668:Kidney; D013539:Simian virus 40 / Q000528:radiation effects; D014466:Ultraviolet Rays; D014779:Virus Replication
D004279:DNA, Viral