title: Selective chemical modification of malate dehydrogenase. N-ethylmaleimide modification of active center sulfhydryl residues.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000645:Ammonium Sulfate; D000818:Animals; D001665:Binding Sites; D002247:Carbon Isotopes; D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002621:Chemistry; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D003545:Cysteine; D004586:Electrophoresis; D005033:Ethylmaleimide; D006863:Hydrogen-Ion Concentration; D007094:Imides; D007700:Kinetics; D008291:Malate Dehydrogenase / Q000032:analysis / Q000037:antagonists & inhibitors / Q000302:isolation & purification; D008433:Mathematics; D008931:Mitochondria, Muscle / Q000201:enzymology*; D009206:Myocardium / Q000166:cytology / Q000201:enzymology*; D009243:NAD; D011485:Protein Binding; D013053:Spectrophotometry; D013386:Succinates; D013552:Swine; D014461:Ultracentrifugation
chemical_list: D000596:Amino Acids; D002247:Carbon Isotopes; D007094:Imides; D013386:Succinates; D009243:NAD; D008291:Malate Dehydrogenase; D003545:Cysteine; D005033:Ethylmaleimide; D000645:Ammonium Sulfate