Study of the one-step growth curve of equine infectious anemia virus by immunofluorescence.
Primary horse leukocyte cultures were inoculated with 2 or 10 50% tissue culture infective doses (TCID(50)) of equine infectious anemia (EIA) virus per cell, and the titer of cell-associated and fluid-phase virus was determined from 1 to 72 hr postinoculation (PI). Cover slips were collected from 4 to 72 hr PI and stained for EIA viral antigen by the indirect immunofluorescent (FA) technique. Viral replication was detected after a latent period of approximately 18 to 24 hr and reached peak titers of approximately 10(4.5) to 10(6) TCID(50)/0.5 ml from 48 to 72 hr PI. The fluid phase contained 10(1) to 10(2) TCID(50)/0.5 ml more virus than the cells. Viral antigen was first detected by FA from 18 to 24 hr PI. Approximately 75% of the cells contained antigen in their cytoplasm 72 hr PI. The FA technique is a sensitive method for detecting EIA virus in horse leukocyte cultures.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D003593:Cytoplasm / Q000276:immunology; D004859:Equine Infectious Anemia / Q000175:diagnosis; D005455:Fluorescent Antibody Technique; D006736:Horses; D004860:Infectious Anemia Virus, Equine / Q000254:growth & development*; D007962:Leukocytes / Q000276:immunology / Q000382:microbiology; D012698:Serologic Tests; D013997:Time Factors; D014779:Virus Replication
D000956:Antigens, Viral