Dose responsiveness and variation among inbred strains of mice in production of interferon after treatment with poly (I)-poly (C) (poly-D-lysine) complexes.
Poly-d-lysine forms a stochiometric complex with poly(I) . poly(C) which has a higher T(m) (83 C in 0.15 m NaCl) than the uncomplexed double-stranded polyribo-nucleotide (63 C). The complex was superior to poly(I) . poly(C) alone as an interferon inducer in vivo. Significant serum interferon titers were attained in Swiss mice during a 24-hr period after intraperitoneal injection of 10 to 300 mug of poly(I) . poly(C) [1.0 poly-d-lysine] complex, at concentrations of 100 to 1,000 mug/ml. The serum interferon responses (average and maximum titers) of a series of inbred strains of mice to a single intraperitoneal injection of 100 mug of complex decreased in the order: Swiss > DBA/2 > C3H > BALB/c > CF-1 > AKR, C57Bl/6, NZB > SJL > NZW and varied by a factor of approximately 100 from the most to the least responsive.
D000818:Animals; D001681:Biological Assay; D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002621:Chemistry; D005823:Genetics; D007274:Injections, Intraperitoneal; D007372:Interferons / Q000032:analysis / Q000096:biosynthesis* / Q000097:blood; D007739:L Cells; D008239:Lysine / Q000008:administration & dosage; D051379:Mice; D008815:Mice, Inbred Strains / Q000187:drug effects / Q000378:metabolism*; D008970:Molecular Weight; D011070:Poly I-C / Q000008:administration & dosage; D011119:Polynucleotides / Q000008:administration & dosage*; D013045:Species Specificity; D013057:Spectrum Analysis; D013997:Time Factors; D014466:Ultraviolet Rays; D014721:Vesicular stomatitis Indiana virus
D011119:Polynucleotides; D007372:Interferons; D008239:Lysine; D011070:Poly I-C