Distribution of pyrimidine oligonucleotides in strands L and H of Bacillus subtilis DNA.
The distribution of pyrimidine oligodeoxynucleotide clusters in L and H strands of Bacillus subtilis DNA separated by methylated albumin-Kieselguhr has been determined. Preparations of native and single-stranded DNA were degraded with diphenylamine in formic acid, and the released isostichs with the general formula of Py(n)P(n+1) were separated on DEAE-cellulose by chain length. Eleven isostichs were obtained for strands L and H in unequal proportions. Each isostich fraction was subfractionated by base composition on DEAE-cellulose at pH 3.0. 61 Pyrimidine oligonucleotide clusters were separated from the H strand and only 46 from the L strand. The findings show a higher degree of asymmetry between the strands in the distribution of cytosine-rich clusters as compared with thymine-rich clusters. The longest cytosine oligodeoxynucleotide present in both strands is of chain length 5. There is an unusually high distribution of thymine oligodeoxynucleotides of length 5-11. Up to chain length 6, the distribution of thymine oligodeoxynucleotides between the strands is about equal; from chain length 7 to 11 they occur predominantly in the H strand.
D000588:Amines; D001412:Bacillus subtilis; D002845:Chromatography; D002848:Chromatography, DEAE-Cellulose; D003597:Cytosine Nucleotides / Q000032:analysis*; D004269:DNA, Bacterial / Q000032:analysis* / Q000302:isolation & purification; D004277:DNA, Single-Stranded / Q000302:isolation & purification; D005561:Formates; D006868:Hydrolysis; D008745:Methylation; D010759:Phosphorus Isotopes; D011119:Polynucleotides / Q000032:analysis / Q000302:isolation & purification; D012709:Serum Albumin; D012822:Silicon Dioxide; D013053:Spectrophotometry; D013942:Thymine Nucleotides / Q000032:analysis*; D014466:Ultraviolet Rays
D000588:Amines; D003597:Cytosine Nucleotides; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D005561:Formates; D010759:Phosphorus Isotopes; D011119:Polynucleotides; D012709:Serum Albumin; D013942:Thymine Nucleotides; D012822:Silicon Dioxide