Comparison of the ribonucleic acid polymerases of two rhabdoviruses, Kern Canyon virus and vesicular stomatitis virus.
A ribonucleic acid (RNA)-dependent RNA polymerase has been demonstrated in Kern Canyon virus (KCV) particles. The RNA product of the KCV polymerase hybridizes to KCV viral RNA. The properties of this viral enzyme have been characterized and compared with those of vesicular stomatitis virus (VSV). RNA polymerases from both viruses require similar conditions of temperature, pH, and detergent and magnesium concentrations for maximal synthesis of RNA. The RNA polymerase contained in the virion of KCV was more dependent on the presence of a sulfhydryl agent than was the VSV enzyme. Under optimal conditions, the specific activity of the VSV polymerase is about twenty-five times as great as that of KCV.
D000818:Animals; D002247:Carbon Isotopes; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D006224:Cricetinae; D003470:Culture Media; D006150:Guanine Nucleotides / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007668:Kidney; D008274:Magnesium / Q000494:pharmacology; D009693:Nucleic Acid Hybridization; D000644:Quaternary Ammonium Compounds; D012316:RNA Nucleotidyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D012367:RNA, Viral / Q000096:biosynthesis; D013045:Species Specificity; D013395:Sucrose; D013431:Sulfates; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D013696:Temperature; D014316:Tritium; D014500:Uracil Nucleotides / Q000378:metabolism; D014529:Uridine / Q000378:metabolism; D014721:Vesicular stomatitis Indiana virus / Q000201:enzymology* / Q000302:isolation & purification / Q000378:metabolism / Q000472:pathogenicity; D014713:Viruses, Unclassified / Q000201:enzymology* / Q000302:isolation & purification / Q000378:metabolism / Q000472:pathogenicity
D002247:Carbon Isotopes; D003470:Culture Media; D006150:Guanine Nucleotides; D000644:Quaternary Ammonium Compounds; D012367:RNA, Viral; D013431:Sulfates; D013439:Sulfhydryl Reagents; D014500:Uracil Nucleotides; D014316:Tritium; D013395:Sucrose; D012316:RNA Nucleotidyltransferases; D008274:Magnesium; D014529:Uridine