Studies on the diversity of inositol-containing yeast phospholipids: incorporation of 2-deoxyglucose into lipid.
Seven inositol-containing phospholipids in Saccharomyces cerevisiae can be resolved by two-dimensional chromatography on silicic acid-impregnated paper. Four of these lipids are stable to mild alkaline methanolysis; one of these has already been characterized as a mannosyl di(inositolphosphoryl) ceramide. Addition of labeled 2-deoxy-d-glucose to a growing culture results in label appearing in five lipids, some of which are alkali-stable and have the same R(F) values as the alkali-stable inositol-containing phospholipids. These lipids are labeled rapidly. The deoxyglucose is incorporated intact, probably in a glycosidic link, since the deoxyglucose is released by mild acid treatment.
D001345:Autoradiography; D002247:Carbon Isotopes; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D006601:Hexoses / Q000378:metabolism*; D006868:Hydrolysis; D007294:Inositol / Q000378:metabolism*; D008055:Lipids / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D010710:Phosphates / Q000378:metabolism; D010716:Phosphatidylinositols / Q000032:analysis / Q000096:biosynthesis; D010743:Phospholipids / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D010759:Phosphorus Isotopes; D012440:Saccharomyces / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism; D013107:Sphingolipids / Q000032:analysis / Q000096:biosynthesis; D014316:Tritium
D002247:Carbon Isotopes; D003470:Culture Media; D006601:Hexoses; D008055:Lipids; D010710:Phosphates; D010716:Phosphatidylinositols; D010743:Phospholipids; D010759:Phosphorus Isotopes; D013107:Sphingolipids; D014316:Tritium; D007294:Inositol; D005947:Glucose