title: Action of haemophilus endodeoxyribonuclease on biologically active deoxyribonucleic acid.
abstract: An enzyme similar to that described by Smith and Wilcox (15) for Haemophilus influenzae which attacks foreign deoxyribonucleic acid (DNA) but not its own has been isolated and purified from H. parainfluenzae. The enzyme degrades foreign DNA to limited sizes and can destroy the transforming activity of H. influenzae and Bacillus subtilis DNA. The enzyme can also destroy the biological activity of H. influenzae phage and prophage DNA. On the other hand, the H. influenzae endodeoxyribonuclease can destroy the transforming activity of H. parainfluenzae DNA but not its own DNA. It also attacks B. subtilis DNA and its transforming activity.
mesh_terms: D000645:Ammonium Sulfate; D001412:Bacillus subtilis; D001435:Bacteriophages; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D004269:DNA, Bacterial / Q000037:antagonists & inhibitors*; D004279:DNA, Viral / Q000037:antagonists & inhibitors*; D003851:Deoxyribonucleases / Q000302:isolation & purification / Q000494:pharmacology*; D006190:Haemophilus / Q000201:enzymology*; D006193:Haemophilus influenzae; D009691:Nucleic Acid Denaturation / Q000187:drug effects*; D018184:Paramyxoviridae Infections; D013045:Species Specificity; D014170:Transformation, Genetic / Q000187:drug effects; D014783:Viscosity
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral; D003851:Deoxyribonucleases; D000645:Ammonium Sulfate