Effect of pleiotropic carbohydrate mutations (ctr) on tryptophan catabolism.
The pleiotropic ctr mutation has been shown to affect tryptophan uptake and tryptophanase formation. Genetic reversions are of two types: (i) complete, restoring to wild type, located at 46 to 47 min; (ii) partial, restoring only tryptophanase synthesis, located at 73 min. In some strains the effect of ctr mutations could be reversed by cyclic adenosine 3',5'-monophosphate (cAMP) plus tryptophan. A mutant producing tryptophanase constitutively was suppressed by a ctr mutation. Production of tryptophanase in this suppressed strain was not restored by the addition of cAMP, but required cAMP plus tryptophan.
D050260:Carbohydrate Metabolism; D002247:Carbon Isotopes; D002874:Chromosome Mapping; D003470:Culture Media; D000242:Cyclic AMP / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D005827:Genetics, Microbial; D006868:Hydrolysis; D009153:Mutagens; D009154:Mutation; D009604:Nitrosoguanidines; D013057:Spectrum Analysis; D013489:Suppression, Genetic; D014161:Transduction, Genetic; D014364:Tryptophan / Q000378:metabolism*; D014366:Tryptophan Oxygenase / Q000096:biosynthesis / Q000378:metabolism
D002247:Carbon Isotopes; D003470:Culture Media; D009153:Mutagens; D009604:Nitrosoguanidines; D014364:Tryptophan; D000242:Cyclic AMP; D014366:Tryptophan Oxygenase