Growth of murine cytomegalovirus in various cell lines.
Murine cytomegalovirus (MCMV) was capable of infecting and replicating in both primary and continuous cell lines obtained from various species. In African green monkey kidney (BSC-1) cells, primary rabbit kidney cells, and baby hamster kidney (BHK-21) cells, there were cytopathic effects (CPE) and virus replication upon initial exposure of cells to virus. In primary fetal sheep brain (FSB) cells, L cells, and rabbit kidney (RK-13) cells, it was necessary to subculture the infected cells one or more times before appearance of CPE and replication of virus. In the case of the infected FSB cultures, it was found that the virus effect could be induced if subculturing were accomplished by trypsinization but did not occur if cells were subcultured by scraping. FSB-grown virus replicated better in FSB than in mouse embryo fibroblast (MEF) cells. The CPE produced in all of the above cell lines was similar to that observed in MEF infected with MCMV. The virus grown in different cell lines was completely neutralized when mixed with several reference sera prepared in rabbits or mice. The populations of virions released from infected MEF and FSB cells were compared by isopycnic centrifugation in potassium tartrate, and no differences were revealed in the buoyant densities of the populations. Human embryonic brain cells, human embryonic kidney cells, a human lung fibroblast cell strain (WI-38), HeLa, and Hep-2 were not susceptible to MCMV.
D000818:Animals; D001921:Brain; D002277:Carcinoma; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D003470:Culture Media; D046508:Culture Techniques; D003587:Cytomegalovirus / Q000254:growth & development* / Q000472:pathogenicity; D003588:Cytopathogenic Effect, Viral; D005347:Fibroblasts; D005615:Freezing; D000882:Haplorhini; D006367:HeLa Cells; D006801:Humans; D007106:Immune Sera; D007668:Kidney; D007739:L Cells; D007822:Laryngeal Neoplasms; D008168:Lung; D051379:Mice; D011188:Potassium; D011817:Rabbits; D012756:Sheep; D013644:Tartrates; D013936:Thymidine; D014316:Tritium; D014357:Trypsin; D014465:Ultrasonics; D014732:Vibration; D014779:Virus Replication
D003470:Culture Media; D007106:Immune Sera; D013644:Tartrates; D014316:Tritium; D014357:Trypsin; D011188:Potassium; D013936:Thymidine