Identification of products of the uridinediphospho-N-acetyl-D-glucosamine oxidoreductase system from Citrobacter freundii ATCC 10053.
Uridinediphospho-N-acetyl glucosamine is converted to one or more uridine-diphospho-4-keto-6-deoxy-2-acetamidohexose derivatives by an enzyme from Citrobacter freundii ATCC 10053. Borohydride reduction of reaction products followed by acid hydrolysis yielded several amino sugars. Two of these were identified as fucosamine and quinovosamine by chromatography and ninhydrin degradation.
D000085:Acetates; D000429:Alcohol Oxidoreductases; D001894:Borohydrides; D002247:Carbon Isotopes; D055598:Chemical Phenomena; D002621:Chemistry; D002852:Chromatography, Ion Exchange; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D003124:Colorimetry; D004586:Electrophoresis; D004925:Escherichia / Q000201:enzymology*; D005944:Glucosamine; D006595:Hexosamines / Q000138:chemical synthesis; D006868:Hydrolysis; D007700:Kinetics; D009702:Nucleoside Diphosphate Sugars / Q000138:chemical synthesis; D010084:Oxidation-Reduction; D010504:Periodic Acid; D013053:Spectrophotometry; D014466:Ultraviolet Rays; D014500:Uracil Nucleotides / Q000138:chemical synthesis
D000085:Acetates; D001894:Borohydrides; D002247:Carbon Isotopes; D006595:Hexosamines; D009702:Nucleoside Diphosphate Sugars; D014500:Uracil Nucleotides; D010504:Periodic Acid; D000429:Alcohol Oxidoreductases; D005944:Glucosamine