Four-directional-development thin-layer chromatography of lipids using trimethyl borate.
Solvent mixtures containing trimethyl borate virtually eliminated the pronounced interconversion of 1,2- and 1,3-dipalmitins during their resolution by thin-layer chromatography on Silica Gel G. With trimethyl borate, an average of 1-2% of 1,2-dipalmitin was converted to 1,3-dipalmitin. A four-directional-development TLC procedure incorporating trimethyl borate resolves cholesteryl glucoside, ceramides, monogalactosyl diglyceride, 1- and 2-monopalmitin, palmitic acid, cholesterol, 1,2- and 1,3-dipalmitin, tripalmitin, methyl palmitate, cholesteryl palmitate, beta-carotene and some of its degradation products, squalene, and tetracosane. Digalactosyl diglyceride, phosphatidic acid, phosphatidylglucose, cerebrosides, and other phospholipids remain near the origin. A mixture containing triolein, 1,2- and 1,3-diolein, 1- and 2- monoolein, oleic acid, and cholesterol was resolved in one dimension. A similar series of palmitic-containing neutral lipids was also resolvable in one dimension. These procedures were applied to the TLC of human sera lipids.
D000085:Acetates; D000535:Aluminum; D001554:Benzene; D001888:Boric Acids; D002338:Carotenoids / Q000302:isolation & purification; D002518:Ceramides / Q000302:isolation & purification; D002554:Cerebrosides / Q000302:isolation & purification; D055598:Chemical Phenomena; D002621:Chemistry; D002784:Cholesterol / Q000302:isolation & purification; D002855:Chromatography, Thin Layer; D004952:Esters / Q000302:isolation & purification; D005019:Ethyl Ethers; D005690:Galactose; D005782:Gels; D005898:Glass; D005989:Glycerides / Q000302:isolation & purification; D006017:Glycolipids / Q000302:isolation & purification; D006027:Glycosides / Q000302:isolation & purification; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006949:Hyperlipidemias / Q000097:blood; D007536:Isomerism; D008055:Lipids / Q000097:blood / Q000302:isolation & purification*; D008297:Male; D008722:Methods; D009829:Oleic Acids / Q000302:isolation & purification; D010169:Palmitic Acids / Q000302:isolation & purification; D010578:Petroleum; D010743:Phospholipids / Q000302:isolation & purification; D012822:Silicon Dioxide; D013185:Squalene / Q000302:isolation & purification; D014280:Triglycerides / Q000302:isolation & purification
D000085:Acetates; D001888:Boric Acids; D002518:Ceramides; D002554:Cerebrosides; D004952:Esters; D005019:Ethyl Ethers; D005782:Gels; D005989:Glycerides; D006017:Glycolipids; D006027:Glycosides; D008055:Lipids; D009829:Oleic Acids; D010169:Palmitic Acids; D010578:Petroleum; D010743:Phospholipids; D014280:Triglycerides; D002338:Carotenoids; D012822:Silicon Dioxide; D013185:Squalene; D002784:Cholesterol; D000535:Aluminum; D001554:Benzene; D005690:Galactose