Defective interfering particles of poliovirus. I. Isolation and physical properties.
A class of defective interfering (DI) poliovirus particles has been identified. The first was found as a contaminant of a viral stock; others have been isolated by serial passage at a high multiplicity of infection. The DI particles are less dense than standard virus and sediment more slowly. Their ribonucleic acid (RNA) sediments more slowly than standard RNA and has a higher electrophoretic mobility. Competition hybridization experiments with double-stranded viral RNA indicate that DI RNA is 80 to 90% of the length of standard RNA. The proteins of DI particles are indistinguishable from those of standard poliovirus.
D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D004589:Electrophoresis, Disc; D006367:HeLa Cells; D007930:Leucine / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010759:Phosphorus Isotopes; D017955:Poliovirus / Q000032:analysis / Q000254:growth & development / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D012526:Satellite Viruses / Q000032:analysis / Q000302:isolation & purification; D013395:Sucrose; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D014762:Viral Interference; D014764:Viral Proteins / Q000032:analysis; D014776:Virus Cultivation
D002247:Carbon Isotopes; D002712:Chlorides; D010759:Phosphorus Isotopes; D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium; D002586:Cesium; D013395:Sucrose; D007930:Leucine; D014529:Uridine