title: Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. IV. Characterization of the simian virus 40 ribonucleic acid species induced by wild-type simian virus 40 and by the nondefective hybrid virus, Ad2 + ND 1 .
abstract: Ad2(+)ND(1), a nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid virus, has been previously shown to contain a small segment of the SV40 genome covalently linked to Ad2 deoxyribonucleic acid (DNA). The SV40 portion of this hybrid virus has been characterized by relating the SV40-specific ribonucleic acid (RNA) sequences transcribed from the Ad2(+)ND(1) DNA to those transcribed from the DNA of SV40 itself. RNA-DNA hybridization-competition studies indicate that the SV40 component of Ad2(+)ND(1) consists of some, but not all, of that part of the SV40 genome which is transcribed early, i.e., prior to viral DNA replication, in SV40 lytic infection.
mesh_terms: D000256:Adenoviridae / Q000032:analysis*; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001483:Base Sequence; D002247:Carbon Isotopes; D002460:Cell Line; D046508:Culture Techniques; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis / Q000302:isolation & purification; D005815:Genetic Code; D005827:Genetics, Microbial; D000882:Haplorhini; D006801:Humans; D006824:Hybridization, Genetic; D007668:Kidney; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D013539:Simian virus 40 / Q000032:analysis* / Q000276:immunology / Q000472:pathogenicity; D013936:Thymidine; D014776:Virus Cultivation
chemical_list: D000956:Antigens, Viral; D002247:Carbon Isotopes; D004279:DNA, Viral; D012367:RNA, Viral; D013936:Thymidine