Lyophilization of cell-free Marek's disease herpesvirus and a herpesvirus from turkeys.
Cell extracts of the JM and GA strains of Marek's disease herpesvirus and the FC 126 strain of turkey herpesvirus were lyophilized with various stabilizers. Much higher virus titers were obtained with stabilizer than without stabilizer. Titers increased even further in the case of the Marek's disease virus strains by the addition of a chelating agent, disodium ethylenediaminetetraacetate.
D000418:Albumins; D001355:Alpharetrovirus / Q000254:growth & development; D000818:Animals; D002474:Cell-Free System; D002645:Chickens; D046508:Culture Techniques; D004492:Edetic Acid; D005612:Freeze Drying; D005947:Glucose; D006564:Herpesviridae / Q000254:growth & development; D007668:Kidney; D008722:Methods; D008892:Milk; D010710:Phosphates; D012965:Sodium Chloride; D013395:Sucrose; D014422:Turkeys
D000418:Albumins; D010710:Phosphates; D012965:Sodium Chloride; D013395:Sucrose; D004492:Edetic Acid; D005947:Glucose