Dye uptake assay: an efficient and sensitive method for human interferon titration.
Currently, human interferon (IF) assays are generally performed by plaque reduction or visual cytopathic effect methods. Both are time-consuming, subjective in interpretation, and, in the case of the latter, relatively insensitive. An adaption of the dye uptake method for human IF titration which uses foreskin-derived fibroblasts and vesicular stomatitis virus is described. This assay is reproducible and sensitive (1 unit = 3 international units). By direct comparison, however, it is somewhat less sensitive than the plaque reduction assay (1 unit = 1.1 international units). This assay is especially recommended for use in needed clinical investigations of human IF because of its technical simplicity, allowing efficient handling of large numbers of specimens.
D002452:Cell Count; D002478:Cells, Cultured / Q000378:metabolism; D004396:Coloring Agents / Q000378:metabolism*; D003470:Culture Media; D046508:Culture Techniques; D003588:Cytopathogenic Effect, Viral; D005347:Fibroblasts; D006801:Humans; D007372:Interferons / Q000032:analysis*; D007962:Leukocytes; D008297:Male; D008722:Methods; D009522:Newcastle disease virus; D010413:Penis; D014721:Vesicular stomatitis Indiana virus
D004396:Coloring Agents; D003470:Culture Media; D007372:Interferons