Components of adenovirus-simian virus 40 hybrid viruses.
The DNA and protein components of the adenovirus 7-simian virus (SV)40 hybrid virus "E46(+)" have been investigated. The Ad.7-SV40 hybrid DNA genome could be distinguished from nonhybrid Ad. 7 genome on the basis of its molecular weight in an alkaline sucrose gradient. The hybrid genome could also be separated from nonhybrid adenovirus 2 DNA when purified transcapsidant adenovirus 2(+t7) (Ad.7-SV40 DNA within an Ad.2 capsid) was studied. The presence and amount of SV40 DNA in the hybrid virus genome was determined by DNA-RNA hybridization. The results presented suggest: (i) that about 10% of the particles in the E46(+) preparations contained SV40 DNA covalently linked to adenovirus DNA; (ii) that the hybrid virus genome has a molecular weight 10-12% lower than that of nonhybrid virus, consistent with a deletion of adenovirus DNA; and (iii) that the hybrid virus contained only 40-50% of a total SV40 genome. The protein components of the Ad.7-SV40 hybrid virus were examined by acrylamide gel electrophoresis in sodium dodecyl sulfate. No significant quantitative or qualitative differences between the proteins of hybrid and nonhybrid adenovirus were demonstrated.
D000256:Adenoviridae / Q000032:analysis*; D002277:Carcinoma; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D004279:DNA, Viral / Q000032:analysis*; D004586:Electrophoresis; D004622:Embryo, Mammalian; D004926:Escherichia coli / Q000201:enzymology; D006801:Humans; D006824:Hybridization, Genetic; D007668:Kidney; D008970:Molecular Weight; D009062:Mouth Neoplasms; D009693:Nucleic Acid Hybridization; D012316:RNA Nucleotidyltransferases; D012333:RNA, Messenger / Q000302:isolation & purification; D012367:RNA, Viral; D013539:Simian virus 40 / Q000032:analysis*; D014316:Tritium; D014529:Uridine; D014764:Viral Proteins / Q000032:analysis*
D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium; D012316:RNA Nucleotidyltransferases; D014529:Uridine