Manganous ion as a spin label in studies of mitochondrial uptake of manganese.
Manganous ion (Mn(2+)) has been used as a spin label for studies of divalent cation uptake by rat liver mitochondria. Spin exchange, observed in the electron paramagnetic resonance (EPR) spectrum of a fraction of the transported Mn(2+), shows that this fraction is bound in regions of high local concentration within the mitochondria. The average separation of manganese ions in that fraction is estimated to be 4.0 +/-0.6 A at the time of greatest concentration.
D000818:Animals; D001693:Biological Transport, Active; D004492:Edetic Acid; D004578:Electron Spin Resonance Spectroscopy; D008345:Manganese / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D051381:Rats
D008345:Manganese; D004492:Edetic Acid