Continuous-flow ultracentrifugation of canine distemper virus and infectious canine hepatitis virus.
The use of a Spinco L-4 zonal centrifuge with the B-XVI continuous-flow rotor for the purification of canine distemper and infectious canine hepatitis viruses is described. Up to 68 liters of virus was processed at one time. Infectious canine hepatitis virus was found to band at 39% sucrose and canine distemper virus banded between 32 and 48% sucrose. The virus was concentrated 10-fold, and the purity of the virus, as measured by protein concentration, was increased by 99%.
D006516:Adenoviruses, Canine / Q000254:growth & development / Q000302:isolation & purification*; D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002501:Centrifugation, Zonal; D004217:Distemper Virus, Canine / Q000254:growth & development / Q000302:isolation & purification*; D004285:Dogs; D007106:Immune Sera; D005779:Immunodiffusion; D007668:Kidney; D008722:Methods; D011817:Rabbits; D013395:Sucrose
D007106:Immune Sera; D013395:Sucrose