Simple method for the isolation of encephalomyocarditis virus ribonucleic acid.
A simple purification procedure for encephalomyocarditis virus is described, yielding material suitable for extraction of viral ribonucleic acid for use as messenger in animal cell-free systems.
D000818:Animals; D001345:Autoradiography; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D046508:Culture Techniques; D004589:Electrophoresis, Disc; D004686:Encephalomyocarditis virus / Q000032:analysis; D006018:Glycols; D008715:Methionine; D008722:Methods; D051379:Mice; D009374:Neoplasms, Experimental; D011479:Protamines; D012333:RNA, Messenger; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate; D013395:Sucrose; D013431:Sulfates; D013460:Sulfur Isotopes; D014776:Virus Cultivation
D002712:Chlorides; D006018:Glycols; D011479:Protamines; D012333:RNA, Messenger; D012367:RNA, Viral; D013431:Sulfates; D013460:Sulfur Isotopes; D002586:Cesium; D012967:Sodium Dodecyl Sulfate; D013395:Sucrose; D008715:Methionine