Influence of polycations on the interaction between poliovirus multistranded ribonucleic acid and HeLa cells.
In contrast to single-stranded viral ribonucleic acid (RNA), poliovirus multistranded RNA (RI-RNA) is poorly adsorbed to suspended HeLa cells in the absence of polycations. In the presence of 20 mug of diethylaminoethyl (DEAE) dextran per ml and other polycations, 90% or more of the RI-RNA is adsorbed to HeLa cells within 2 min at 37 C. However, only 30% of the RI-RNA label penetrates into the cells independent of the concentration of DEAE dextran applied. DEAE dextran is adsorbed almost quantitatively to HeLa cells within 3 min at 37 C. Most of the DEAE dextran remains bound to the cell membrane and available for binding of RI-RNA for 15 min at 37 C.
D000327:Adsorption; D001120:Arginine / Q000494:pharmacology; D001665:Binding Sites; D002462:Cell Membrane / Q000187:drug effects; D002712:Chlorides; D002850:Chromatography, Gel; D003911:Dextrans / Q000494:pharmacology; D005021:Ethylamines / Q000494:pharmacology; D006367:HeLa Cells; D008094:Lithium; D008239:Lysine / Q000494:pharmacology; D009952:Ornithine / Q000494:pharmacology; D017955:Poliovirus / Q000472:pathogenicity*; D011108:Polymers / Q000494:pharmacology*; D012367:RNA, Viral / Q000302:isolation & purification; D012260:Ribonucleases; D013696:Temperature; D013997:Time Factors; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D002712:Chlorides; D003911:Dextrans; D005021:Ethylamines; D011108:Polymers; D012367:RNA, Viral; D014316:Tritium; D001120:Arginine; D008094:Lithium; D009952:Ornithine; D012260:Ribonucleases; D008239:Lysine; D014529:Uridine