title: DNA synthesis by RNA-containing tumor viruses.
abstract: Murine leukemia (Rauscher and Moloney strains) and sarcoma (Kirsten strain) virions, as well as the mammary tumor virus of mice, contain an RNA-dependent DNA polymerase. Optimal incorporation of deoxyribonucleoside triphosphates occurs at a critical detergent (Triton X-100) concentration (0.010-0.014%). At higher than optimal detergent concentrations the virion is seen to be disrupted and enzyme activity is lost. The virion, enzymatic activity, and newly synthesized DNA all cosediment in a sucrose gradient. Thus far the enzymatic activity has been found only in RNA viruses that have oncogenic properties.
mesh_terms: D002499:Centrifugation, Density Gradient; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004279:DNA, Viral / Q000096:biosynthesis*; D017988:Gammaretrovirus / Q000201:enzymology*; D009052:Leukemia Virus, Murine / Q000201:enzymology; D008324:Mammary Tumor Virus, Mouse / Q000201:enzymology; D008979:Moloney murine leukemia virus / Q000201:enzymology; D009522:Newcastle disease virus / Q000201:enzymology; D009975:Orthomyxoviridae / Q000201:enzymology; D010222:Parainfluenza Virus 1, Human / Q000201:enzymology; D011925:Rauscher Virus / Q000201:enzymology; D012136:Respiratory Syncytial Viruses / Q000201:enzymology; D013501:Surface-Active Agents
chemical_list: D004279:DNA, Viral; D013501:Surface-Active Agents; D004254:DNA Nucleotidyltransferases