Isolation and properties of poliovirus minus strand ribonucleic acid.
Poliovirus minus strands were purified from double-stranded polio ribonucleic acid. The minus strands have a base ratio complementary to that of the viral ribonucleic acid and are not infectious.
D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D002845:Chromatography; D003902:Detergents; D005990:Glycerol; D006367:HeLa Cells; D009691:Nucleic Acid Denaturation; D010710:Phosphates; D010759:Phosphorus Isotopes; D017955:Poliovirus / Q000254:growth & development; D011134:Polysaccharides; D012367:RNA, Viral / Q000302:isolation & purification / Q000494:pharmacology; D014316:Tritium; D014529:Uridine
D002712:Chlorides; D003902:Detergents; D010710:Phosphates; D010759:Phosphorus Isotopes; D011134:Polysaccharides; D012367:RNA, Viral; D014316:Tritium; D002586:Cesium; D005990:Glycerol; D014529:Uridine