Purification of the Sabin strain of poliovirus type I through treatment with sarkozyl.
Sodium dodecyl sulfate was found to aggregate virions from the Sabin Lsc2ab strain of poliovirus type 1. Aggregation was prevented by high ionic strength buffers. A procedure is described for the rapid purification of the virus through the use of sarkozyl.
D000596:Amino Acids; D002021:Buffers; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D003609:Dactinomycin; D003902:Detergents; D004492:Edetic Acid; D004589:Electrophoresis, Disc; D006367:HeLa Cells; D006851:Hydrochloric Acid; D008722:Methods; D017955:Poliovirus / Q000302:isolation & purification*; D012964:Sodium; D012965:Sodium Chloride; D013395:Sucrose; D013431:Sulfates; D014316:Tritium; D014529:Uridine; D014776:Virus Cultivation
D000596:Amino Acids; D002021:Buffers; D002247:Carbon Isotopes; D003902:Detergents; D013431:Sulfates; D014316:Tritium; D003609:Dactinomycin; D012965:Sodium Chloride; D013395:Sucrose; D004492:Edetic Acid; D012964:Sodium; D006851:Hydrochloric Acid; D014529:Uridine