Physical properties of lactic dehydrogenase-elevating virus and its ribonucleic acid.
Infectious lactic dehydrogenase-elevating virus propagated in primary cultures of mouse peritoneal macrophages in the presence of (3)H-uridine and isolated by isopycnic centrifugation was found to have a density of 1.12 g/cm(3). Ribonucleic acid extracted from the virus by treatment with sodium dodecyl sulfate was single stranded with a sedimentation coefficient of approximately 48S.
D000818:Animals; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D002501:Centrifugation, Zonal; D004121:Dimethyl Sulfoxide; D006358:Hot Temperature; D007739:L Cells; D007770:L-Lactate Dehydrogenase; D008264:Macrophages; D051379:Mice; D009691:Nucleic Acid Denaturation; D010537:Peritoneum; D012328:RNA Viruses / Q000032:analysis / Q000254:growth & development; D012367:RNA, Viral / Q000032:analysis; D012190:Retroviridae / Q000032:analysis / Q000254:growth & development; D012260:Ribonucleases; D012967:Sodium Dodecyl Sulfate; D013936:Thymidine; D014316:Tritium; D014529:Uridine; D014776:Virus Cultivation
D012367:RNA, Viral; D014316:Tritium; D012967:Sodium Dodecyl Sulfate; D007770:L-Lactate Dehydrogenase; D012260:Ribonucleases; D013936:Thymidine; D014529:Uridine; D004121:Dimethyl Sulfoxide