Ribonucleic acid polymerase induced in cells infected with Sendai virus.
A ribonucleic acid (RNA)-dependent RNA polymerase was induced in chick embryo fibroblast cells after infection with Sendai virus (parainfluenza 1 virus). The enzyme was associated with the microsomal fraction of infected cells and reached maximum detectable activity at 18 hr after virus infection. The activity of the enzyme in vitro was dependent on the presence of added magnesium ions and all four nucleoside triphosphates and was not inhibited by actinomycin D. The RNA synthesized by the enzyme in vitro was sensitive to ribonuclease and consisted of a complex mixture of RNA species including 34S, 24S, and 18S components. Similar RNA components were detected in the microsomal fraction of Sendai virus-infected cells by labeling with (3)H-uridine from 17 to 18 hr postinfection in the presence of actinomycin D. Of the RNA synthesized by Sendai virus-induced RNA polymerase in vitro, 98% became insensitive to ribonuclease after annealing with RNA extracted from purified Sendai virus particles.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D046508:Culture Techniques; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004790:Enzyme Induction; D005347:Fibroblasts / Q000201:enzymology; D006824:Hybridization, Genetic; D006863:Hydrogen-Ion Concentration; D007477:Ions; D008274:Magnesium / Q000494:pharmacology; D008861:Microsomes / Q000201:enzymology; D009711:Nucleotides / Q000494:pharmacology; D010222:Parainfluenza Virus 1, Human / Q000472:pathogenicity*; D010759:Phosphorus Isotopes; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D012316:RNA Nucleotidyltransferases / Q000378:metabolism*; D012367:RNA, Viral / Q000096:biosynthesis / Q000302:isolation & purification; D012260:Ribonucleases / Q000378:metabolism; D013395:Sucrose; D013696:Temperature; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D007477:Ions; D009711:Nucleotides; D010759:Phosphorus Isotopes; D012367:RNA, Viral; D014316:Tritium; D003609:Dactinomycin; D013395:Sucrose; D012313:RNA; D003513:Cycloheximide; D012316:RNA Nucleotidyltransferases; D012260:Ribonucleases; D008274:Magnesium; D014529:Uridine