In vivo and in vitro synthesis of human rhinovirus type 2 ribonucleic acid.
HeLa cells infected with human rhinovirus type 2 synthesize a mixture of single-and double-stranded ribonucleic acid (RNA). The RNA synthesized by the membrane-bound RNA polymerase complex in vitro is also a mixture of single- and double-stranded RNA, whereas the deoxycholate-treated RNA polymerase complex synthesized only double-stranded RNA. Although twice as much cell-associated viral RNA is synthesized in vivo at 34 C than at 37 C, there is no difference in the rate of RNA synthesized in vitro at 34 C and 37 C by the polymerase complex. The RNA polymerase complex, after treatment with deoxycholate, sediments as a broad peak with an average sedimentation value of 120S.
D001647:Bile Acids and Salts; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D004589:Electrophoresis, Disc; D006150:Guanine Nucleotides / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D012316:RNA Nucleotidyltransferases / Q000378:metabolism; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D012229:Rhinovirus / Q000254:growth & development / Q000378:metabolism*; D013501:Surface-Active Agents; D013696:Temperature; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D001647:Bile Acids and Salts; D006150:Guanine Nucleotides; D012367:RNA, Viral; D013501:Surface-Active Agents; D014316:Tritium; D012316:RNA Nucleotidyltransferases; D014529:Uridine