Effect of norepinephrine on glucose metabolism in glioblastoma and neuroblastoma cells in cell culture.
The addition of norepinephrine to cultured glioblastoma cells results in an inhibition of uptake of radioactivity from D-[2-(3)H]glucose, D-[1-(14)C]glucose, D-[2-(14)C]glucose, and D-[6-(14)C]glucose. In addition, if the glioblastoma cells are previously labeled with these substrates, norepinephrine causes an increase in the release of radioactivity. These effects were not observed with cultured neuroblastoma cells. It is suggested that the breakdown of glycogen is activated by norepinephrine as a result of an increase in 3':5'-cyclic AMP.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002245:Carbon Dioxide / Q000096:biosynthesis; D002247:Carbon Isotopes; D002999:Clone Cells; D000242:Cyclic AMP / Q000378:metabolism; D003864:Depression, Chemical; D005909:Glioblastoma / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006003:Glycogen / Q000378:metabolism; D051379:Mice; D008851:Micropore Filters; D009447:Neuroblastoma / Q000378:metabolism*; D009638:Norepinephrine / Q000494:pharmacology*; D051381:Rats; D013806:Theophylline / Q000494:pharmacology; D014316:Tritium
D002247:Carbon Isotopes; D014316:Tritium; D002245:Carbon Dioxide; D006003:Glycogen; D013806:Theophylline; D000242:Cyclic AMP; D005947:Glucose; D009638:Norepinephrine