DNA polymerase activities associated with smooth membranes and ribosomes from rat liver and hepatoma cytoplasm.
Deoxyribonucleic acid polymerase activity that prefers denatured DNA primer concentrates with the smooth membranes during sucrose gradient centrifugation of rat liver and hepatoma cytoplasmic extracts. The activity in this fraction is eightfold higher in hepatoma tissue. Deoxyribonucleic acid polymerase activity that prefers native DNA primer concentrates with the free ribosome fraction from both tissues.
D000643:Ammonium Chloride; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000201:enzymology*; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D004247:DNA / Q000096:biosynthesis; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000096:biosynthesis; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D008110:Liver Extracts / Q000032:analysis; D008113:Liver Neoplasms; D008297:Male; D008566:Membranes / Q000201:enzymology*; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000201:enzymology; D009691:Nucleic Acid Denaturation; D009711:Nucleotides / Q000378:metabolism; D011189:Potassium Chloride; D051381:Rats; D012270:Ribosomes / Q000201:enzymology*
D004273:DNA, Neoplasm; D008110:Liver Extracts; D009711:Nucleotides; D000643:Ammonium Chloride; D011189:Potassium Chloride; D004247:DNA; D004254:DNA Nucleotidyltransferases