Transformation of African green monkey kidney cells by irradiated adenovirus 7-simian virus 40 hybrid.
The ability of adenovirus 7-simian virus 40 (SV40) hybrid (strain LL "E-46") to replicate decreased exponentially as a function of the amount of gamma-irradiation; the ability to induce SV40 and adenovirus 7 T antigen decreased at a much slower rate. Nevertheless, the virus was still able to transform African green monkey kidney cells at a radiation dosage that had completely destroyed its replication ability. All transformed colonies were positive for SV40 T antigen but were negative for adenovirus 7 T antigen. The adenovirus 7-SV40 hybrid transformed cells were superinfectible with SV40 virus. Two of the three transformed cell populations apparently did not sensitize hamsters against the appearance SV40 primary tumors, thus suggesting a deficiency in the SV40 transplantation antigen.
D000256:Adenoviridae / Q000528:radiation effects*; D000818:Animals; D000941:Antigens; D002471:Cell Transformation, Neoplastic; D006224:Cricetinae; D046508:Culture Techniques; D004622:Embryo, Mammalian; D000882:Haplorhini; D006801:Humans; D006824:Hybridization, Genetic; D007668:Kidney; D009374:Neoplasms, Experimental / Q000276:immunology; D011831:Radiation Genetics; D013539:Simian virus 40 / Q000528:radiation effects*; D014779:Virus Replication / Q000528:radiation effects
D000941:Antigens