Genetic relatedness of type 1 and type 2 herpes simplex viruses.
The extent of homology between herpes simplex virus(1) and(2) (HSV-1 and HSV-2) deoxyribonucleic acid (DNA) was measured in two ways: (i) by determination of the relative rate of hybridization of labeled HSV-1 and HSV-2 DNA to excess unlabeled HSV-1 or HSV-2 DNA immobilized on filters and (ii) by determination of the rate of hybridization of labeled HSV-1 and HSV-2 DNA to excess unlabeled HSV-1 or HSV-2 DNA in solution. Approximately 40% of HSV-1 and HSV-2 DNA is homologous at hybridization temperatures 25 C below the melting temperature (T(m)) of HSV DNA (liquid-filter annealing). Lowering the temperature to 34 C below the T(m) increased the extent of homology to 46% (liquid annealing). The extent of base-pairing in HSV-1-HSV-2 heteroduplex DNA was determined by thermal chromatography on hydroxyapatite. Heteroduplexes of HSV-1 and HSV-2 DNA eluted in a single peak whose midpoint (Te(50)) was 10 C below that of the homoduplex. Conspicuously absent were heteroduplexes that eluted at more than 15 C below the Te(50) of the homoduplex. The data indicate the existence of a variable region of DNA (54%) with very little, if any, homology and an invariable region (46%) with relatively good (85%) matching of base pairs.
D001483:Base Sequence; D002247:Carbon Isotopes; D002277:Carcinoma; D002460:Cell Line; D002845:Chromatography; D004277:DNA, Single-Stranded / Q000302:isolation & purification; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D003851:Deoxyribonucleases; D006801:Humans; D006882:Hydroxyapatites; D007822:Laryngeal Neoplasms; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D009695:Nucleic Acid Renaturation; D018139:Simplexvirus / Q000032:analysis / Q000145:classification*; D013696:Temperature; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D002247:Carbon Isotopes; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D006882:Hydroxyapatites; D014316:Tritium; D003851:Deoxyribonucleases; D013936:Thymidine