Identification and genetic characterization of mutants of bacteriophage T4 defective in the ability to induce exonuclease A.
A mutant of bacteriophage T4 unable to induce exonuclease A has been isolated. The mutation responsible for this defect maps between genes 39 and 56, in a region of the chromosome devoid of other known markers. Four deletion mutants lacking part of the genome located between genes 39 and 56 also fail to induce exonuclease A. The ability of all of these mutants to replicate suggests that exonuclease A is not essential for replication of phage T4.
D002474:Cell-Free System; D002848:Chromatography, DEAE-Cellulose; D002874:Chromosome Mapping; D003090:Coliphages / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D003597:Cytosine Nucleotides; D004269:DNA, Bacterial / Q000378:metabolism; D003673:Defective Viruses; D003851:Deoxyribonucleases / Q000096:biosynthesis* / Q000378:metabolism; D004790:Enzyme Induction; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism; D005827:Genetics, Microbial; D006918:Hydroxyurea / Q000494:pharmacology; D008780:Methyltransferases / Q000378:metabolism; D009154:Mutation; D009691:Nucleic Acid Denaturation; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D011995:Recombination, Genetic; D013762:Tetrahydrofolate Dehydrogenase / Q000378:metabolism; D014779:Virus Replication
D003597:Cytosine Nucleotides; D004269:DNA, Bacterial; D013762:Tetrahydrofolate Dehydrogenase; D008780:Methyltransferases; D003851:Deoxyribonucleases; D010744:Phosphoric Monoester Hydrolases; D006918:Hydroxyurea