Preparation of labeled aflatoxins with high specific activities.
Resting cells of Aspergillus parasiticus ATCC 15517 were used to prepare highly labeled aflatoxins from labeled acetate. High synthetic activity in growing cells was evidenced only during 40 to 70 hr of incubation. Glucose was required for high incorporation efficiency, whereas the concentration of the labeled acetate determined the specific activity of the product. When labeled acetate was continuously added to maintain a concentration near but not exceeding 10 mm, in a culture containing 30 g of glucose per liter, 2% of its labels could be recovered in the purified aflatoxins which have a specific activity more than three times that of the labeled acetate.
D000085:Acetates / Q000378:metabolism; D000348:Aflatoxins / Q000096:biosynthesis* / Q000302:isolation & purification; D000645:Ammonium Sulfate; D001230:Aspergillus / Q000254:growth & development / Q000378:metabolism*; D001554:Benzene; D002247:Carbon Isotopes; D002725:Chloroform; D002855:Chromatography, Thin Layer; D003470:Culture Media; D005782:Gels; D005947:Glucose / Q000378:metabolism; D012822:Silicon Dioxide; D013997:Time Factors
D000085:Acetates; D000348:Aflatoxins; D002247:Carbon Isotopes; D003470:Culture Media; D005782:Gels; D012822:Silicon Dioxide; D002725:Chloroform; D005947:Glucose; D001554:Benzene; D000645:Ammonium Sulfate