Determination of the gene sequence of poliovirus with pactamycin.
By examination of the virus-specific polypeptides formed after the addition of pactamycin, an inhibitor of protein chain initiation, to infected cells, it has been possible to tentatively locate the virus coat proteins at the amino terminus of the large, virus-specific protein precursor, and, therefore, to assign the coat protein cistron to the 5' end of the RNA.
D000595:Amino Acid Sequence; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D003864:Depression, Chemical; D004586:Electrophoresis; D005815:Genetic Code; D006367:HeLa Cells; D010442:Peptide Chain Initiation, Translational; D017955:Poliovirus / Q000032:analysis* / Q000187:drug effects / Q000378:metabolism; D011487:Protein Conformation; D012270:Ribosomes / Q000187:drug effects; D013997:Time Factors; D014316:Tritium; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism
D000903:Antibiotics, Antineoplastic; D002247:Carbon Isotopes; D014764:Viral Proteins; D014316:Tritium