title: Accumulation of untranslated lactose-specific messenger ribonucleic acid during catabolite repression in Escherichia coli.
abstract: When Escherichia coli K-12 Hfr.H was induced to synthesize beta-galactosidase in the presence of glucose, an untranslated lactose-specific mRNA (lac-mRNA), protected from decay, was found to accumulate progressively within the cells. The lac-mRNA accumulation was unaffected by the carbon source on which the cells had been grown before the induction. The amount of the lac-mRNA available for translation was affected by catabolite repression and 3':5'-cyclic AMP, but it remained unclear whether this was a direct effect on the formation of the lac-mRNA or a consequence of the effect on its translation.
mesh_terms: D000166:Acridines / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology; D004790:Enzyme Induction; D004794:Enzyme Repression; D004926:Escherichia coli / Q000378:metabolism*; D005696:Galactosidases / Q000096:biosynthesis / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism*; D014529:Uridine / Q000378:metabolism
chemical_list: D000166:Acridines; D012333:RNA, Messenger; D000242:Cyclic AMP; D005696:Galactosidases; D007930:Leucine; D005947:Glucose; D005990:Glycerol; D014529:Uridine