Leukemia viruses associated with mouse myeloma cells.
Myeloma cells derived from BALB/c and C3H mice show evidence of infection by a murine leumemia virus. The immunoglobulin-producing myelomas secrete an RNA-containing virus with a density of 1.20 to 1.22 gm/cm(3). RNA with a sedimentation coefficient of 74 S in 0.1 M sodium sodium chloride has been isolated from secreted virus particles and has a base composition similar to that found for other murine leukemia virus RNA. An intracellular virus particle has been partially purified and has a density of 1.29 to 1.32 gm/cm(3). Both extracellular and intracellular virus particles contain the leukemia virus group-specific antigen.
D000818:Animals; D002247:Carbon Isotopes; D002460:Cell Line; D046508:Culture Techniques; D003902:Detergents; D007074:Immunoglobulin G / Q000032:analysis; D009052:Leukemia Virus, Murine / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D051379:Mice; D008854:Microscopy, Electron; D009101:Multiple Myeloma / Q000382:microbiology*; D010759:Phosphorus Isotopes; D012367:RNA, Viral / Q000302:isolation & purification; D014316:Tritium; D014776:Virus Cultivation
D002247:Carbon Isotopes; D003902:Detergents; D007074:Immunoglobulin G; D010759:Phosphorus Isotopes; D012367:RNA, Viral; D014316:Tritium