title: Regulation of protein synthesis in HeLa cells. 3. Inhibition during poliovirus infection.
abstract: The mechanism of inhibition of host cell protein synthesis by poliovirus has been studied by examining the behavior of host polyribosomes and polyribosome-associated messenger ribonucleic acid (mRNA). Virus infection appears to result in a specific inhibition of the initiation of translation of host cell mRNA. The site of inhibition does not appear to be mRNA itself. Human cells respond to virus infection by producing a factor antagonistic to the virus inhibition which promotes the initiation of host mRNA translation. The production of the host factor is sensitive to actinomycin; however, the behavior of the host cell factor and that of host mRNA appear distinctly different.
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D002247:Carbon Isotopes; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004589:Electrophoresis, Disc; D005815:Genetic Code; D006367:HeLa Cells / Q000378:metabolism*; D006801:Humans; D007930:Leucine / Q000378:metabolism; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D017955:Poliovirus / Q000472:pathogenicity*; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis; D012333:RNA, Messenger / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D013395:Sucrose; D014316:Tritium; D014529:Uridine / Q000378:metabolism
chemical_list: D000596:Amino Acids; D002247:Carbon Isotopes; D002712:Chlorides; D011506:Proteins; D012333:RNA, Messenger; D014316:Tritium; D003609:Dactinomycin; D002586:Cesium; D013395:Sucrose; D003513:Cycloheximide; D007930:Leucine; D014529:Uridine