title: Studies on the ultrastructure of Bordetella pertussis. I. Morphology, origin, and biological activity of structures present in the extracellular fluid of liquid cultures of Bordetella pertussis.
abstract: Two distinct particles have been recognized in the extracellular fluid of B. pertussis cultures. Both appeared to arise from the surface (cell wall) of the organism. One of these, a membranous particle, seemed to derive from long projections on the organism composed of the outer membrane of the cell wall. The second particle, a fine filament, was not readily comparable with any previously described bacterial structure. The two particles could be separated from each other by gradient centrifugation in CsCl. Lymphocytosis-promoting factor and histamine-sensitizing activity were only associated with fractions containing the fine filaments.
mesh_terms: D000818:Animals; D001886:Bordetella pertussis / Q000166:cytology*; D002462:Cell Membrane; D002473:Cell Wall; D002499:Centrifugation, Density Gradient; D003470:Culture Media; D004926:Escherichia coli; D008218:Lymphocytosis; D008722:Methods; D051379:Mice; D008854:Microscopy, Electron
chemical_list: D003470:Culture Media