Expression of lactate and malate dehydrogenases in tumors induced by SV40 and 7,12-dimethylbenz(a)anthracene.
Isozyme patterns of lactate and malate dehydrogenases were studied in tumors induced by SV40 and 7,12-dimethylbenz(a)anthracene and in established cultures of cells from these tumors. The expression of B polypeptide subunits of lactate dehydrogenase is suppressed similarly by both agents. This may be due to inactivation of the gene at the locus determining the B polypeptide subunit. Malate dehydrogenase isozyme patterns are not changed significantly by the virus or the carcinogen.
D000818:Animals; D001551:Benz(a)Anthracenes; D002471:Cell Transformation, Neoplastic; D006224:Cricetinae; D046508:Culture Techniques; D004592:Electrophoresis, Starch Gel; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D008291:Malate Dehydrogenase / Q000032:analysis*; D009132:Muscles / Q000201:enzymology; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000201:enzymology*; D013539:Simian virus 40
D001551:Benz(a)Anthracenes; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase