Complex lipid requirements for detergent-solubilized phosphoacetylmuramyl-pentapeptide translocase from Micrococcus luteus.
Phospho-MurNAc-pentapeptide translocase activity in the membrane of M. luteus was lost upon addition of the detergent, Triton X-100, but could be restored by addition of lipid fractions to the assay. By assay in the presence of lipid, the activity of the Triton-solubilized enzyme could be measured. The synthesis of C(55)-isoprenyl-P-P-MurNAc-pentapeptide from UDP-MurNAc-pentapeptide required C(55)-isoprenyl-P, and was stimulated by a neutral lipid. The exchange reaction of UDP-MurNAc-pentapeptide with UMP required a polar lipid fraction, but the reaction was not affected by C(55)-isoprenyl-P or the neutral lipid. Thus, measurement of activity of the detergent-solubilized enzyme requires addition of three lipids, the lipid substrate (C(55)-isoprenyl-P), the neutral lipid, and a polar lipid.
D002247:Carbon Isotopes; D002725:Chloroform; D002850:Chromatography, Gel; D004789:Enzyme Activation; D006020:Glycopeptides; D007700:Kinetics; D008055:Lipids / Q000494:pharmacology*; D000432:Methanol; D008837:Micrococcus / Q000187:drug effects / Q000201:enzymology*; D010455:Peptides; D010714:Phosphatidylethanolamines / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000494:pharmacology; D010756:Phosphoric Acids; D012995:Solubility; D013501:Surface-Active Agents / Q000494:pharmacology; D013729:Terpenes; D014166:Transferases / Q000302:isolation & purification; D014500:Uracil Nucleotides
D002247:Carbon Isotopes; D006020:Glycopeptides; D008055:Lipids; D010455:Peptides; D010714:Phosphatidylethanolamines; D010716:Phosphatidylinositols; D010756:Phosphoric Acids; D013501:Surface-Active Agents; D013729:Terpenes; D014500:Uracil Nucleotides; D002725:Chloroform; D014166:Transferases; D000432:Methanol