Preparation of type 2 herpes simplex virus complement-fixing antigen.
Comparable complement-fixing antigens of type 1 and type 2 herpes simplex virus were produced by extraction of infected African green monkey cells with 0.85% NaCl which was buffered at pH 9.0 with 0.05 m glycine-NaOH. The optimal antigen dilutions were higher in titrations against hyperimmune animal sera than in titrations against human sera. Complement-fixing antibody to type 2 herpes antigen was detected in 5 of 17 sera from healthy humans.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000941:Antigens / Q000302:isolation & purification*; D000956:Antigens, Viral / Q000302:isolation & purification; D002460:Cell Line; D003168:Complement Fixation Tests; D003588:Cytopathogenic Effect, Viral; D003937:Diagnosis, Differential; D005998:Glycine; D000882:Haplorhini; D006561:Herpes Simplex / Q000175:diagnosis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera; D007120:Immunochemistry; D007668:Kidney; D011817:Rabbits; D012703:Serotyping; D018139:Simplexvirus / Q000276:immunology* / Q000472:pathogenicity; D012965:Sodium Chloride; D012972:Sodium Hydroxide; D014465:Ultrasonics; D014732:Vibration
D000906:Antibodies; D000941:Antigens; D000956:Antigens, Viral; D007106:Immune Sera; D012965:Sodium Chloride; D012972:Sodium Hydroxide; D005998:Glycine