title: Effects of starvation, refeeding, and fat feeding on adipocyte ghost adenyl cyclase activity.
abstract: Basal adenyl cyclase activity and its response to epinephrine and glucagon were studied in isolated adipocyte ghosts obtained from fed, starved, refed, and fat-diet-adapted rats. Epinephrine stimulation of adenyl cyclase was significantly increased in fasted rats, but the glucagon response did not change. Rats fasted for 48 hr and refed a high carbohydrate, low fat diet for 48 or 96 hr showed no differences from chow-fed animals in either basal or hormone-stimulated adenyl cyclase activity. Rats adapted to a high fat, low carbohydrate diet showed an initial and transitory increase in basal activity but a progressive loss of epinephrine- and glucagon-stimulated enzyme activities. The loss in hormone responsiveness correlated well with a decrease in hormone-stimulated lipolysis of fat pads and was associated with a significant increase in fat cell diameter.
mesh_terms: D000255:Adenosine Triphosphate; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology*; D002477:Cells / Q000187:drug effects / Q000201:enzymology; D000242:Cyclic AMP; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D004822:Epididymis / Q000378:metabolism; D004837:Epinephrine / Q000494:pharmacology; D005215:Fasting; D005934:Glucagon / Q000494:pharmacology; D005990:Glycerol / Q000096:biosynthesis; D008297:Male; D010759:Phosphorus Isotopes; D051381:Rats; D014316:Tritium
chemical_list: D004040:Dietary Carbohydrates; D004041:Dietary Fats; D010759:Phosphorus Isotopes; D014316:Tritium; D000255:Adenosine Triphosphate; D005934:Glucagon; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D005990:Glycerol; D004837:Epinephrine