Effect of rifampicin and two of its derivatives on cells infected with Moloney sarcoma virus.
It is shown that rifampicin, and especially the related antibiotic 2',6'-dimethyl-N(4')-benzyl-N(4')- [desmethyl]rifampicin (DMB-rifampicin) can inhibit focus formation by Moloney sarcoma virus on BALB/3T3 tissue cultures. At 10 mug/ml DMB-rifampicin totally inhibits focus formation while reducing virus replication by at least a factor of fifty and cell proliferation by only a factor of three. These observations, taken together with those of others, suggest a role for an RNA-dependent DNA polymerase and the gene for its synthesis both in normal cell processes and in the transformation process.
D000818:Animals; D001593:Benzyl Compounds / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004254:DNA Nucleotidyltransferases / Q000096:biosynthesis; D051379:Mice; D008979:Moloney murine leukemia virus / Q000187:drug effects*; D012293:Rifampin / Q000494:pharmacology*; D012513:Sarcoma, Experimental; D014170:Transformation, Genetic / Q000187:drug effects; D014721:Vesicular stomatitis Indiana virus; D014779:Virus Replication / Q000187:drug effects
D001593:Benzyl Compounds; D004254:DNA Nucleotidyltransferases; D012293:Rifampin