Genetic analysis of simian virus 40. IV. Inhibited transformation of Balb-3T3 cells by a temperature-sensitive early mutant.
The temperature-sensitive early mutant, ts(*)101, was characterized during productive infection in monkey cells, and the results are presented in an accompanying paper. This paper demonstrates that although 101 mutant virions adsorb normally to confluent Balb/3T3 mouse cells at both permissive (33 C) and restrictive (38.5 C) temperatures, T antigen synthesis and transformation, abortive and stable, are inhibited at both temperatures (host-range inhibition). T antigen synthesis is temperature sensitive, whereas abortive and stable transformation are not. Clones of 101-transformed Balb/3T3 cells were isolated, and virus was rescued from all clones at both permissive and restrictive temperatures. The rescued virus was as temperature sensitive as the original transforming 101 virions.
D000327:Adsorption; D000818:Animals; D000956:Antigens, Viral / Q000378:metabolism; D002460:Cell Line / Q000382:microbiology*; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells / Q000382:microbiology; D005827:Genetics, Microbial; D000882:Haplorhini; D007668:Kidney; D051379:Mice; D009154:Mutation; D010222:Parainfluenza Virus 1, Human / Q000254:growth & development; D013539:Simian virus 40 / Q000276:immunology / Q000302:isolation & purification; D013696:Temperature; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D000956:Antigens, Viral; D014316:Tritium; D014529:Uridine