Inactivation of T antigen-forming capacites of simian virus 40 and adenovirus 12 by ultraviolet irradiation.
Methods to measure T antigen-forming capacities of simian virus 40 (SV40) and adenovirus 12 (Ad12) were investigated, and a method to measure the capacity in terms of T antigen-forming units was employed by the use of cytosine arabinoside. Plaque-forming units and T antigen-forming units of SV40, SV40 deoxyribonucleic acid, or Ad12 were inactivated by ultraviolet (UV) irradiation at the same rate, roughly following a single-hit curve. T-antigen formation by UV-irradiated SV40 and Ad12 was enhanced in cells multiply infected and in cells in a growing state. These observations showed that it was difficult or impossible to estimate the size of the gene for T antigen by UV inactivation.
D000256:Adenoviridae / Q000276:immunology* / Q000472:pathogenicity / Q000528:radiation effects; D000818:Animals; D000941:Antigens / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured / Q000254:growth & development; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D006224:Cricetinae; D003561:Cytarabine / Q000494:pharmacology; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000302:isolation & purification / Q000528:radiation effects; D005455:Fluorescent Antibody Technique; D005796:Genes; D005827:Genetics, Microbial; D000882:Haplorhini; D006801:Humans; D007106:Immune Sera; D007668:Kidney; D008856:Microscopy, Fluorescence; D010636:Phenols; D011817:Rabbits; D011830:Radiation Effects; D013539:Simian virus 40 / Q000276:immunology* / Q000472:pathogenicity / Q000528:radiation effects; D012964:Sodium; D013431:Sulfates; D013997:Time Factors; D014466:Ultraviolet Rays
D000941:Antigens; D002712:Chlorides; D004279:DNA, Viral; D007106:Immune Sera; D010636:Phenols; D013431:Sulfates; D003561:Cytarabine; D002586:Cesium; D012964:Sodium