Yeast membrane vesicles: isolation and general characteristics.
Yeast membrane vesicles are formed when packed yeast are ground manually in a porcelain mortar and pestle with glass beads (0.2 mm diameter). These vesicles can be separated from the other components of the grinding mixture by a combination of centrifugation steps and elution from a column of the same glass beads (0.2 mm diameter). Isolated vesicles are osmotically sensitive, contain cytoplasmic components, and have energy-independent transport function. They are unable to metabolize glucose, but have respiratory function which is thought to be associated with intravesicular mitochondria. Invertase and oligomycin-insensitive adenosine triphosphatase are present in lysed vesicle preparations, and the appropriateness of these enzyme activities as membrane markers is discussed.
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors; D000596:Amino Acids / Q000378:metabolism; D002458:Cell Fractionation; D002462:Cell Membrane / Q000032:analysis* / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000032:analysis; D005690:Galactose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D002479:Inclusion Bodies / Q000032:analysis* / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008858:Microscopy, Phase-Contrast; D008928:Mitochondria / Q000378:metabolism; D009840:Oligomycins / Q000494:pharmacology; D009996:Osmotic Fragility; D010101:Oxygen Consumption; D011189:Potassium Chloride / Q000494:pharmacology; D012440:Saccharomyces / Q000032:analysis / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D013393:Sucrase / Q000032:analysis
D000596:Amino Acids; D009840:Oligomycins; D011189:Potassium Chloride; D003576:Electron Transport Complex IV; D013393:Sucrase; D000251:Adenosine Triphosphatases; D005947:Glucose; D005690:Galactose