Inhibition of the metabolism of 25-hydroxycholecalciferol by actinomycin D and cycloheximide.
Actinomycin D (or cycloheximide) administered prior to radioactive 25-hydroxycholecalciferol blocks the metabolism of 25-hydroxycholecalciferol to polar metabolites that accumulate in intestinal tissue, while it does not prevent the 25-hydroxylation of vitamin D(3) in the liver. Actinomycin D given after radioactive 25-hydroxycholecalciferol does not inhibit 25-hydroxycholecalciferol metabolism. These results indicate that 25-hydroxycholecalciferol must interact with the nuclei of cells to bring about the production of an enzyme(s) that converts it to its polar metabolites.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002762:Cholecalciferol / Q000378:metabolism*; D002845:Chromatography; D003513:Cycloheximide / Q000494:pharmacology*; D003609:Dactinomycin / Q000494:pharmacology*; D003864:Depression, Chemical
D002762:Cholecalciferol; D003609:Dactinomycin; D003513:Cycloheximide