Relationship between virus-induced cellular deoxyribonucleic acid synthesis and transformation by simian virus 40.
The fraction of cells in a confluent 3T3 cell monolayer induced by simian virus 40 infection to replicate deoxyribonucleic acid and divide corresponds to those cells which eventually become transformed. Virus-induced cells were partially separated from noninduced cells by sedimentation through Ficoll gradients. Three- to eightfold higher transformation frequencies were obtained with those cells that began to synthesize cellular deoxyribonucleic acid and divide shortly after simian virus 40 infection as compared to noninduced cells.
D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured / Q000166:cytology / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis*; D004261:DNA Replication; D010710:Phosphates / Q000378:metabolism; D010759:Phosphorus Isotopes; D011134:Polysaccharides; D013539:Simian virus 40 / Q000472:pathogenicity*; D013395:Sucrose; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium
D010710:Phosphates; D010759:Phosphorus Isotopes; D011134:Polysaccharides; D014316:Tritium; D013395:Sucrose; D004247:DNA; D013936:Thymidine