Ribonuclease-sensitive deoxyribonucleic acid polymerase activity in uninfected rat cells and rat cells infected with Rous sarcoma virus.
Rat cells infected with the B77 strain of avian sarcoma virus [R(B77) cells] produced no virus-like particles but contained information for the production of infectious B77 virus. (3)H-labeled deoxyribonucleic acid (DNA) product of the B77 virus endogenous DNA polymerase system was used to determine the relative amounts of B77 virus-specific ribonucleic acid (RNA) in B77 virus-infected chicken and R(B77) cells. R(B77) cells were found to contain much less B77 virus RNA than did B77 virus-infected chicken cells. Ribonuclease-sensitive DNA polymerase activity was present in high-speed pellet fractions from Nonidet extracts of B77 virus-infected rat cells. Similar preparations from some uninfected rat cells contained lesser amounts of a similar ribonuclease-sensitive DNA polymerase activity. The endogenous template for the DNA polymerase activity in high-speed pellet fractions from R(B77) cells was not related to B77 virus RNA or to RNA of a rat C-type virus. The DNA product of the endogenous DNA polymerase in high-speed pellet fractions of R(B77) cells hybridized to a small extent with RNA from the same fraction and to a similar extent with RNA from uninfected rat cells.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000254:growth & development / Q000378:metabolism / Q000472:pathogenicity*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002474:Cell-Free System; D002478:Cells, Cultured / Q000201:enzymology*; D002499:Centrifugation, Density Gradient; D002586:Cesium; D011232:Chemical Precipitation; D002642:Chick Embryo; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004279:DNA, Viral; D005347:Fibroblasts; D005827:Genetics, Microbial; D009693:Nucleic Acid Hybridization; D012313:RNA; D012334:RNA, Neoplasm; D012367:RNA, Viral; D051381:Rats; D012260:Ribonucleases / Q000494:pharmacology; D013395:Sucrose; D013431:Sulfates; D013501:Surface-Active Agents; D013698:Templates, Genetic; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D014779:Virus Replication
D004279:DNA, Viral; D012334:RNA, Neoplasm; D012367:RNA, Viral; D013431:Sulfates; D013501:Surface-Active Agents; D014316:Tritium; D002586:Cesium; D013395:Sucrose; D012313:RNA; D004254:DNA Nucleotidyltransferases; D012260:Ribonucleases; D014529:Uridine