Metabolic studies on retinoic acid in the rat.
The nature of metabolites in the urine arising from differentially labelled retinoic acid was investigated after injection of physiological doses into retinol-deficient rats. Distribution of radioactivity after partition of urine into ether-soluble, acidic and water-soluble fractions revealed that there were at least six metabolites in urine. Of these, the major metabolite(s) was one lacking both C-14 and C-15 of retinoic acid. Enzymic or alkaline hydrolysis of acidic and water-soluble fractions did not release any retinoic acid, thus indicating that retinoyl beta-glucuronide was not present in urine in significant amounts.
D000818:Animals; D002247:Carbon Isotopes; D002338:Carotenoids / Q000378:metabolism* / Q000652:urine; D002845:Chromatography; D005019:Ethyl Ethers; D005966:Glucuronidase; D006851:Hydrochloric Acid; D006868:Hydrolysis; D008297:Male; D051381:Rats; D012822:Silicon Dioxide; D012995:Solubility; D014802:Vitamin A Deficiency / Q000378:metabolism
D002247:Carbon Isotopes; D005019:Ethyl Ethers; D002338:Carotenoids; D012822:Silicon Dioxide; D005966:Glucuronidase; D006851:Hydrochloric Acid