Ribonucleic acid polymerase activity in Sendai virions and nucleocapsid.
After dissociation of purified Sendai virus with the neutral detergent Nonidet P-40 and 2-mercaptoethanol, it catalyzed the incorporation of ribonucleoside triphosphates into an acid-insoluble product. The enzyme activity was associated with viral nucleocapsid as well as whole virions. The reaction product was ribonucleic acid (RNA) which annealed specifically with virion RNA. Sedimentation of the (3)H-RNA reaction product revealed two components, a 45S component with properties of double-stranded RNA and 4 to 6S component which appeared to be mostly single-stranded RNA.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001647:Bile Acids and Salts; D002417:Cattle; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D002645:Chickens; D003851:Deoxyribonucleases; D004912:Erythrocytes / Q000276:immunology; D006386:Hemagglutination Tests; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D008623:Mercaptoethanol; D009698:Nucleoproteins; D010179:Pancreas; D010222:Parainfluenza Virus 1, Human / Q000032:analysis / Q000201:enzymology* / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D012316:RNA Nucleotidyltransferases / Q000378:metabolism*; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification; D012260:Ribonucleases; D013395:Sucrose; D013501:Surface-Active Agents; D014316:Tritium; D014500:Uracil Nucleotides / Q000378:metabolism; D014776:Virus Cultivation
D001647:Bile Acids and Salts; D009698:Nucleoproteins; D012367:RNA, Viral; D013501:Surface-Active Agents; D014500:Uracil Nucleotides; D014316:Tritium; D013395:Sucrose; D008623:Mercaptoethanol; D000255:Adenosine Triphosphate; D012316:RNA Nucleotidyltransferases; D003851:Deoxyribonucleases; D012260:Ribonucleases; D008274:Magnesium