Substitution of milk for serum in the production fo human leukocyte interferon.
The presence of serum in suspensions of Sendai-induced human leukocytes is necessary for the synthesis of significant amounts of interferon. Very little interferon is obtained from serum-free suspensions. Cow's milk or milk casein can substitute for serum in the production of high yields of human leukocyte interferon.
D000650:Amnion; D000818:Animals; D001681:Biological Assay; D002364:Caseins; D002417:Cattle; D002460:Cell Line; D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans; D007106:Immune Sera; D007372:Interferons / Q000032:analysis / Q000096:biosynthesis*; D007962:Leukocytes / Q000378:metabolism*; D008892:Milk; D010222:Parainfluenza Virus 1, Human; D014721:Vesicular stomatitis Indiana virus
D002364:Caseins; D003470:Culture Media; D007106:Immune Sera; D007372:Interferons