Asymmetric template function of microbial deoxyribonucleic acids: transcription of ribosomal and soluble ribonucleic acids.
In Bacillus subtilis and Escherichia coli, 16 and 23S ribosomal ribonucleic acid (rRNA) hybridize exclusively with the heavy (H) strand of methylated albuminkieselguhr (MAK)-fractionated complementary deoxyribonucleic acid (DNA) strands. All the soluble RNA (4S RNA) in B. subtilis and 66 to 75% of the 4S RNA in E. coli also hybridize with the H strand. Interspecific hybridization shows that E. coli 23S rRNA also binds selectively to the DNA H strand of Salmonella typhimurium. The hybridization peak for all three cellular RNA components is specifically located in the late-eluting region of the absorbance profile of the DNA H strand. The early-eluting region of the light (L) strand preferentially inhibits the hybridization between the peak region of the H strand and 23S rRNA. These regions are considered to represent the transcribing sequences and their complements for 23S rRNA in the separated H and L strands of DNA, respectively.
D000418:Albumins; D001412:Bacillus subtilis; D002845:Chromatography; D004269:DNA, Bacterial / Q000302:isolation & purification; D004926:Escherichia coli; D005815:Genetic Code; D005827:Genetics, Microbial; D006824:Hybridization, Genetic; D012329:RNA, Bacterial / Q000302:isolation & purification; D012270:Ribosomes; D012486:Salmonella typhimurium; D012822:Silicon Dioxide; D013698:Templates, Genetic; D014316:Tritium; D014498:Uracil
D000418:Albumins; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D014316:Tritium; D014498:Uracil; D012822:Silicon Dioxide