Characterization of an endonuclease associated with simian virus 40 virions.
An endonucleolytic activity associated with purified simian virus 40 (SV40) virions has been found. The enzyme is present in virions prepared from a number of different host lines. The enzyme is present in all early and late temperature-sensitive mutants examined. Some aspects of the endonucleolytic activity have been examined with SV40 deoxyribonucleic acid as substrate.
D000818:Animals; D002247:Carbon Isotopes; D002460:Cell Line / Q000382:microbiology; D002499:Centrifugation, Density Gradient; D002501:Centrifugation, Zonal; D003168:Complement Fixation Tests; D004279:DNA, Viral / Q000302:isolation & purification / Q000378:metabolism; D004720:Endonucleases / Q000302:isolation & purification* / Q000378:metabolism; D004996:Ethidium; D005827:Genetics, Microbial; D000882:Haplorhini; D007668:Kidney; D009154:Mutation; D009500:Neutralization Tests; D013539:Simian virus 40 / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D013696:Temperature; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D002247:Carbon Isotopes; D004279:DNA, Viral; D014316:Tritium; D004720:Endonucleases; D004996:Ethidium; D013936:Thymidine