Immunofluorescent localisation of human alpha feto-protein in fetal and neonatal livers and cultured cells from hepatocellular carcinoma.
The indirect immunofluorescence technique demonstrated that human alpha fetoprotein (AFP) was present in a focal pattern in the cytoplasm of malignant liver cells of patients whose sera contained AFP. A few fibroblastlike cells in tissue culture of liver biopsies from patients with hepato-cellular carcinoma and AFP in their sera also had the protein. The intracellular localisation of human AFP was confirmed by centrifugation of washed, homogenised and ultra-sonically disrupted neonatal liver cells. Examination of livers of fetuses and neonates showed that AFP was present predominantly in the periportal parenchymal cells.
D000328:Adult; D000368:Aged; D000818:Animals; D001706:Biopsy; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002478:Cells, Cultured / Q000032:analysis*; D002498:Centrifugation; D002648:Child; D046508:Culture Techniques; D003593:Cytoplasm / Q000032:analysis; D005260:Female; D005326:Fetal Proteins / Q000032:analysis*; D005333:Fetus; D005347:Fibroblasts / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008099:Liver / Q000032:analysis* / Q000166:cytology; D008113:Liver Neoplasms / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011817:Rabbits; D014465:Ultrasonics
D005326:Fetal Proteins