Epstein-Barr viral antigen in single cell clones of two human leukocytic lines.
A method was devised for obtaining single cell clones of human leukocytic cell lines in the presence of a human placental cell feeder layer. Clones of two lines, LS-B and EB(3), which contain Epstein-Barr viral (EBV) antigen in approximately 1% of cells were tested for EBV antigen. Since all EB(3) clones and LS-B clones contained EBV antigen, it is concluded that in vitro EBV genome is associated with all of the cells.
D000941:Antigens / Q000032:analysis*; D002460:Cell Line; D002999:Clone Cells; D003470:Culture Media; D046508:Culture Techniques; D005455:Fluorescent Antibody Technique; D005827:Genetics, Microbial; D006564:Herpesviridae / Q000276:immunology*; D004854:Herpesvirus 4, Human / Q000276:immunology / Q000472:pathogenicity; D006801:Humans; D007106:Immune Sera; D007962:Leukocytes
D000941:Antigens; D003470:Culture Media; D007106:Immune Sera