Microtiter tests for detecting antibody in bovine serum to parainfluenza 3 virus, infectious bovine rhinotracheitis virus, and bovine virus diarrhea virus.
Microneutralization tests for detection of antibody in bovine serum to three bovine viruses are described. The Madin-Darby bovine kidney cell line was used with parainfluenza 3 virus (PI 3), whereas serially cultivated bovine embryonic kidney cells were used for infectious bovine rhinotracheitis virus and bovine virus diarrhea virus. Comparison of micro-hemagglutination-inhibition (HI) with micro-serum-neutralization (SN) tests for PI 3 showed the SN test to be more sensitive, more specific, and therefore more useful than the HI test for detecting antibody. Although the effect of trypsin-periodate treatment of serum was to reduce the HI titer of numerous sera by a twofold dilution, sufficient evidence could not be found to indicate that nonspecific HI inhibitors to PI 3 are present in bovine sera.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000917:Antibody Formation; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology; D002460:Cell Line; D003470:Culture Media; D003937:Diagnosis, Differential; D003967:Diarrhea / Q000662:veterinary; D006385:Hemagglutination Inhibition Tests; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D006358:Hot Temperature; D007106:Immune Sera; D007114:Immunization; D007668:Kidney; D008722:Methods; D009500:Neutralization Tests; D018184:Paramyxoviridae Infections / Q000175:diagnosis / Q000276:immunology; D010504:Periodic Acid; D012328:RNA Viruses / Q000276:immunology*; D011817:Rabbits; D018108:Respirovirus / Q000276:immunology*; D014357:Trypsin
D000906:Antibodies; D003470:Culture Media; D007106:Immune Sera; D010504:Periodic Acid; D014357:Trypsin