Identification of 1,25-dihydroxycholecalciferol, a form of vitamin D3 metabolically active in the intestine.
A biologically active vitamin D(3) metabolite ("peak V metabolite") more polar than 25-hydroxycholecalciferol has been isolated from chicken intestines in pure form as a mono(trimethylsilyl)ether derivative. The molecule has been identified as 1,25-dihydroxycholecalciferol by means of mass spectrometry, ultraviolet absorption spectrophotometry, and specific chemical reactions.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002645:Chickens; D002762:Cholecalciferol / Q000302:isolation & purification*; D002845:Chromatography; D004987:Ethers; D007422:Intestines / Q000032:analysis*; D013058:Mass Spectrometry; D013053:Spectrophotometry; D014316:Tritium; D014466:Ultraviolet Rays
D004987:Ethers; D014316:Tritium; D002762:Cholecalciferol