title: D-Mannitol utilization in Salmonella typhimurium.
abstract: A biochemical and genetic analysis of d-mannitol metabolism in Salmonella typhimurium indicates that d-mannitol is phosphorylated by the phosphoenolpyruvate-dependent phosphotransferase system. d-Mannitol-1-phosphate is converted to d-fructose-6-phosphate by mannitol-1-phosphate dehydrogenase. Two classes of mannitol mutants are described. Both map at about 115 min on the Salmonella chromosome. Mutants missing mannitol-1-phosphate dehydrogenase activity are mannitol sensitive; i.e., either growth is inhibited or the cells are lysed in the presence of mannitol. In a strain missing adenyl cyclase activity, the mannitol genes require exogenous cyclic adenosine-3',5'-monophosphate for expression.
mesh_terms: D000227:Adenine Nucleotides; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D002247:Carbon Isotopes; D002874:Chromosome Mapping; D003470:Culture Media; D000242:Cyclic AMP / Q000378:metabolism
chemical_list: D000227:Adenine Nucleotides; D002247:Carbon Isotopes; D003470:Culture Media; D000242:Cyclic AMP; D000429:Alcohol Oxidoreductases