Selective association of Sindbis virion proteins with different membrane fractions of infected cells.
Plasma and smooth membranes obtained from chicken embryo cells infected with Sindbis virus were solubilized and subjected to electrophoresis on acrylamide gels. The electrophoretic patterns showed that (i) the major proteins synthesized and associated with plasma membranes from infected cells are virion proteins and (ii) at 4 hr after infection virion proteins are not present at detectable levels in the smooth membranes of the cell.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001103:Arboviruses / Q000254:growth & development*; D002247:Carbon Isotopes; D002458:Cell Fractionation; D002460:Cell Line / Q000382:microbiology; D002462:Cell Membrane / Q000032:analysis* / Q000187:drug effects / Q000201:enzymology / Q000382:microbiology; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D003609:Dactinomycin / Q000494:pharmacology; D004589:Electrophoresis, Disc; D004721:Endoplasmic Reticulum; D005952:Glucose-6-Phosphatase / Q000032:analysis; D009708:Nucleotidases / Q000032:analysis; D010455:Peptides / Q000032:analysis; D011485:Protein Binding; D012845:Sindbis Virus / Q000302:isolation & purification; D013997:Time Factors; D014316:Tritium; D014764:Viral Proteins / Q000096:biosynthesis / Q000302:isolation & purification*
D000596:Amino Acids; D002247:Carbon Isotopes; D010455:Peptides; D014764:Viral Proteins; D014316:Tritium; D003609:Dactinomycin; D009708:Nucleotidases; D005952:Glucose-6-Phosphatase