Preparation and properties of phospholipid bilayers containing rhodopsin.
Purified rhodopsin has been prepared containing less than 1.1 mol of phosphate per mol of protein. The purified rhodopsin has been incorporated into phosphatidylcholine bilayers, and the molecular interactions within the bilayers were investigated by the use of spin-labeled phosphatidylcholines. Rhodopsin appears to inhibit segmental motions of the hydrocarbon chains, an effect similar to that of cholesterol on phospholipid bilayers.
D000818:Animals; D002247:Carbon Isotopes; D002417:Cattle; D002499:Centrifugation, Density Gradient; D002845:Chromatography; D003497:Cyclic N-Oxides; D003624:Darkness; D004578:Electron Spin Resonance Spectroscopy; D005613:Freeze Etching; D006882:Hydroxyapatites; D008027:Light; D008567:Membranes, Artificial; D008854:Microscopy, Electron; D010713:Phosphatidylcholines / Q000528:radiation effects; D010786:Photoreceptor Cells / Q000528:radiation effects; D000644:Quaternary Ammonium Compounds; D011830:Radiation Effects; D012168:Retinal Pigments / Q000302:isolation & purification / Q000528:radiation effects
D002247:Carbon Isotopes; D003497:Cyclic N-Oxides; D006882:Hydroxyapatites; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D000644:Quaternary Ammonium Compounds; D012168:Retinal Pigments