Characterization of the Kilham rat virus.
Kilham rat virus (KRV) was found to grow in a rat nephroma cell line and to form plaques on secondary rat embryo monolayers. The virus was purified by enzymatic treatment and isopycnic cesium chloride sedimentation. KRV bands at a density of 1.41 g/cm(3) in cesium chloride. It contains about 26.5% deoxyribonucleic acid (DNA). The sedimentation coefficient S(20,w) in sucrose gradients was 122 corresponding to a molecular weight of 6.6 x 10(6) daltons. The reaction of formaldehyde with the KRV virion suggests that the DNA in situ is single-stranded. DNA extracted from KRV had a buoyant density of 1.715 g/cm(3) in cesium chloride. The S(20,w) was determined in sucrose gradients to be 16, and the molecular weight was calculated to be approximately 1.7 x 10(6) daltons. The base composition of the DNA is 26.7% adenine, 30.8% thymine, 20.0% guanine, and 22.5% cytosine. On the basis of its noncomplementary nucleotide ratio, melting curve, and the reaction with formaldehyde, the DNA of KRV is believed to be single-stranded.
D000225:Adenine / Q000032:analysis; D000818:Animals; D002247:Carbon Isotopes; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D003596:Cytosine / Q000032:analysis; D004267:DNA Viruses / Q000302:isolation & purification; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D003720:Densitometry; D005557:Formaldehyde; D006147:Guanine / Q000032:analysis; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006358:Hot Temperature; D008854:Microscopy, Electron; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D051381:Rats; D013053:Spectrophotometry; D013395:Sucrose; D013936:Thymidine / Q000378:metabolism; D013941:Thymine / Q000032:analysis; D014316:Tritium; D014713:Viruses, Unclassified / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification; D009396:Wilms Tumor
D002247:Carbon Isotopes; D002712:Chlorides; D004279:DNA, Viral; D014316:Tritium; D005557:Formaldehyde; D002586:Cesium; D013395:Sucrose; D006147:Guanine; D003596:Cytosine; D000225:Adenine; D013941:Thymine; D013936:Thymidine