Characterization of bluetongue virus ribonucleic acid.
An improved purification procedure yielded bluetongue virus free from any single-stranded ribonucleic acid (RNA) component. Double-stranded RNA obtained from purified virus or isolated from infected cells was fractionated into 5 components by means of sucrose gradient sedimentation analysis, and into 10 components by electrophoresis on polyacrylamide gels. The size of these components vary from 0.5 x 10(6) to 2.8 x 10(6) daltons, with a total molecular weight estimate of about 1.5 x 10(7) for the viral nucleic acid. The denaturation of the genome and separation of the resulting fragments are also discussed.
D000179:Acrylates; D000818:Animals; D001819:Bluetongue / Q000209:etiology; D002247:Carbon Isotopes; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002845:Chromatography; D006224:Cricetinae; D004586:Electrophoresis; D005557:Formaldehyde; D005782:Gels; D006358:Hot Temperature; D007202:Indicators and Reagents; D007668:Kidney; D007739:L Cells; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D010636:Phenols; D010759:Phosphorus Isotopes; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D012087:Reoviridae / Q000032:analysis*; D012756:Sheep; D013053:Spectrophotometry; D013395:Sucrose; D014316:Tritium; D014529:Uridine; D014713:Viruses, Unclassified / Q000302:isolation & purification
D000179:Acrylates; D002247:Carbon Isotopes; D005782:Gels; D007202:Indicators and Reagents; D010636:Phenols; D010759:Phosphorus Isotopes; D012367:RNA, Viral; D014316:Tritium; D005557:Formaldehyde; D013395:Sucrose; D014529:Uridine