Potassium: effect on DNA synthesis and multiplication of baby-hamster kidney cells: (cell cycle-membrane potential-synchronization-transformation).
The relations between DNA synthesis, cell multiplication, and external potassium concentration have been investigated in cultured baby-hamster kidney cells. When the potassium concentration was raised from 8 mM to 114 mM by equimolar replacement of sodium, DNA synthesis and cell multiplication were almost completely inhibited. This inhibition was reversible even after 72 hr of incubation in medium with a high concentration of potassium. There is a consistent difference between the cultured cells and polyoma virus-transformed cells in response to high-potassium medium, a higher-potassium concentration being required to inhibit multiplication of polyoma virus-transformed cells to the same extent as that of the nontransformed cells.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D006224:Cricetinae; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis*; D004261:DNA Replication / Q000187:drug effects; D003864:Depression, Chemical; D007668:Kidney / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008858:Microscopy, Phase-Contrast; D009994:Osmolar Concentration; D011120:Polyomavirus / Q000472:pathogenicity; D011188:Potassium / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis
D003470:Culture Media; D012313:RNA; D004247:DNA; D011188:Potassium