Determination of acetyl coenzyme A. Interference by a contaminant in malate dehydrogenase.
Spectrophotometric determinations of acetyl CoA with malate dehydrogenase and citrate synthase are likely to overestimate the amount of acetyl CoA in solutions containing acetoacetyl CoA, since commercial preparations of malate dehydrogenase may contain thiolase.
D000217:Acyltransferases / Q000032:analysis; D002951:Citrates; D003065:Coenzyme A / Q000032:analysis*; D005069:Evaluation Studies as Topic; D008190:Lyases; D008291:Malate Dehydrogenase / Q000032:analysis / Q000592:standards*; D009243:NAD; D013053:Spectrophotometry; D013997:Time Factors; D014466:Ultraviolet Rays
D002951:Citrates; D009243:NAD; D008291:Malate Dehydrogenase; D000217:Acyltransferases; D008190:Lyases; D003065:Coenzyme A