title: Effect of inactivation by hydroxylamine on early functions of poliovirus.
abstract: Evidence is presented that poliovirus particles with a single lethal hit by hydroxylamine do not induce in host cells either inhibition of cellular protein synthesis or viral ribonucleic acid (RNA) replication. The RNA of these viruses is not replicated even if the cells are simultaneously infected with both active and inactivated viruses. The damaged viral RNA seems to have lost both its template function and its function in the translation of normal viral proteins.
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D002247:Carbon Isotopes; D002501:Centrifugation, Zonal; D003513:Cycloheximide / Q000494:pharmacology; D005827:Genetics, Microbial; D006367:HeLa Cells; D006898:Hydroxylamines / Q000494:pharmacology*; D017955:Poliovirus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism / Q000472:pathogenicity; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology; D013395:Sucrose; D013698:Templates, Genetic; D013997:Time Factors; D014529:Uridine / Q000378:metabolism; D014779:Virus Replication
chemical_list: D000596:Amino Acids; D002247:Carbon Isotopes; D006898:Hydroxylamines; D012367:RNA, Viral; D013395:Sucrose; D003513:Cycloheximide; D014529:Uridine