Equilibrium density gradient studies on simian virus 40-yielding variants of the adenovirus type 2-simian virus 40 hybrid population.
The simian virus 40 (SV40)-yielding variants of the adenovirus type 2 (Ad.2)-SV40 hybrid (Ad.2(++)) population were studied by means of fixed-angle equilibrium density gradient centrifugation in cesium chloride. The hybrid virions of the Ad.2(++) high-efficiency yielder population banded at densities of 0.004 g/cm(3) lighter than the nonhybrid Ad.2 virions. The degree of separation of the hybrid particles was sufficient to permit greater than 100-fold relative purification by two cycles of centrifugation. Hybrid particles that produce adenovirus plaques in African green monkey kidney cells by two-hit kinetics (one-hit kinetics when assayed on lawns of nonhybrid adenovirus) were not separable from the particles that yield SV40 virus. The hybrid particle in the Ad.2(++) low-efficiency yielder population was not separable from the nonhybrid Ad.2 virions.
D000256:Adenoviridae / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity; D000818:Animals; D000941:Antigens / Q000032:analysis; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D006224:Cricetinae; D004279:DNA, Viral; D005455:Fluorescent Antibody Technique; D014644:Genetic Variation; D005827:Genetics, Microbial; D000882:Haplorhini; D006801:Humans; D006824:Hybridization, Genetic; D007106:Immune Sera; D007668:Kidney / Q000196:embryology; D013539:Simian virus 40 / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity
D000941:Antigens; D002712:Chlorides; D004279:DNA, Viral; D007106:Immune Sera; D002586:Cesium