In vitro synthesis of procollagen on polysomes.
The major collagenous products synthesized in a cell-free polysome preparation are pro-alpha1 and pro-alpha2, formed in a ratio of 2:1. They are the precursor forms of alpha1 and alpha2 chains of normal connective-tissue collagen that are also formed in small amounts. A procollagen peptidase activity has been demonstrated in the supernatant fraction that can account for the formation of alpha1 and alpha2 chains from their precursors. The polysomal system is activated by a salt extract of reticulocyte ribosomes and is inhibited by aurintricarboxylic acid, suggesting that the polysomes are able to initiate protein synthesis.
D000818:Animals; D001798:Blood Proteins / Q000494:pharmacology; D002417:Cattle; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D002642:Chick Embryo; D002852:Chromatography, Ion Exchange; D003094:Collagen / Q000096:biosynthesis* / Q000302:isolation & purification; D003509:Cyclohexanecarboxylic Acids / Q000494:pharmacology; D004589:Electrophoresis, Disc; D006614:Hindlimb / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D003012:Microbial Collagenase; D010442:Peptide Chain Initiation, Translational; D010447:Peptide Hydrolases / Q000032:analysis; D011392:Proline / Q000378:metabolism; D012343:RNA, Transfer / Q000494:pharmacology; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D014316:Tritium; D014921:Wings, Animal / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*
D001798:Blood Proteins; D003509:Cyclohexanecarboxylic Acids; D014316:Tritium; D003094:Collagen; D012343:RNA, Transfer; D011392:Proline; D010447:Peptide Hydrolases; D003012:Microbial Collagenase