Possible role of microtubules in thyroid secretion.
Colchicine and other microtubule-active agents have been found to block the release, stimulated by either thyroid-stimulating hormone or by dibutyryl cyclic adenosine 3':5'-monophosphate, of (131)I from previously (131)I-labeled mouse thyroid glands in vitro. The time and concentration characteristics of these inhibitors are consistent with their actions on microtubules in other systems. [(3)H]colchicine was also shown to be bound to a soluble 6S protein of bovine thyroid slices similar to the protein identified in other systems as a microtubular subunit. The demonstrated inhibition of colloid droplet formation and absence of an effect on thyroidal adenyl cyclase or cyclic 3':5'-phosphodiesterase suggests a colchicine-sensitive role for microtubules in colloid endocytosis in the thyroid gland.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D001567:Benzocycloheptenes / Q000494:pharmacology; D002417:Cattle; D002850:Chromatography, Gel; D003078:Colchicine / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism; D003864:Depression, Chemical; D006118:Griseofulvin / Q000494:pharmacology; D066298:In Vitro Techniques; D007455:Iodine / Q000378:metabolism; D007456:Iodine Isotopes; D051379:Mice; D008870:Microtubules / Q000187:drug effects; D009940:Organoids / Q000187:drug effects*; D011033:Podophyllin / Q000494:pharmacology; D013961:Thyroid Gland / Q000187:drug effects / Q000378:metabolism*; D013972:Thyrotropin / Q000037:antagonists & inhibitors*; D014316:Tritium; D014747:Vinblastine / Q000494:pharmacology
D001567:Benzocycloheptenes; D007456:Iodine Isotopes; D014316:Tritium; D006118:Griseofulvin; D014747:Vinblastine; D011033:Podophyllin; D013972:Thyrotropin; D007455:Iodine; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D003078:Colchicine