Thymidine kinase of rat liver. Activation by phospholipase C and subcellular distribution in the liver.
1. The thymidine kinase activity of rat liver is greatly enhanced on addition of phospholipase C to the assay mixture. 2. Most of the thymidine kinase activity of the liver is recovered in the mitochondrial and in the impure ;nuclear' fractions. No activity was detected in purified nuclei prepared in high-density sucrose. 3. A substantial thymidine kinase activity could be detected, with the aid of phospholipase C, in all rat tissues examined.
D000818:Animals; D001669:Biochemical Phenomena; D001671:Biochemistry; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000201:enzymology; D003016:Clostridium perfringens / Q000201:enzymology; D003470:Culture Media; D003902:Detergents; D004789:Enzyme Activation; D005615:Freezing; D008099:Liver / Q000201:enzymology*; D008115:Liver Regeneration; D008930:Mitochondria, Liver / Q000201:enzymology; D010740:Phospholipases / Q000494:pharmacology*; D051381:Rats; D013937:Thymidine Kinase / Q000378:metabolism*; D014732:Vibration
D003470:Culture Media; D003902:Detergents; D013937:Thymidine Kinase; D010740:Phospholipases