Membrane dynamics in the action of dibutyryl adenosine 3':5'-cyclic monophosphate and testosterone on mammalian cells.
The first manifestation of reverse transformation, in which a permanent epithelial-like cell is reversibly transformed into a fibroblast-like cell by dibutyryl adenosine cyclic 3':5'-monophosphate, is the disappearance of a set of violently extending and retracting knobbed structures on the cell membrane with consequent tranquilization of the membrane. The cell then elongates to assume a fibroblast-like morphology. Permanently fibroblast-like cells, which have smooth membranes, can be changed into knobbed epithelial-like cells by the addition of either colcemid or cytochalasin B, agents that disorganize one or the other component, respectively, of the microtubular-microfibrillar system. Permanently smoothmembraned, epithelial-like cells also become knobbed by such treatment. All of these knobbed epithelial-like cells can be converted into smooth-membraned fibroblast-like cells by dibutyryl cAMP alone or with the aid of supporting molecules like testosterone. It is proposed that these are general phenomena and that the knobbed epithelial-like forms and the more highly differentiated smooth-membraned fibroblast-like cells represent extreme situations with respect to the degree of organization of the microtubular-microfibrillar system inside the cell.
D000818:Animals; D001688:Biological Products / Q000494:pharmacology; D002087:Butyrates / Q000494:pharmacology; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects*; D006224:Cricetinae; D000242:Cyclic AMP / Q000494:pharmacology*; D003703:Demecolcine / Q000494:pharmacology; D004357:Drug Synergism; D005260:Female; D006367:HeLa Cells / Q000166:cytology / Q000187:drug effects*; D007211:Indoles / Q000494:pharmacology; D008168:Lung / Q000166:cytology / Q000187:drug effects*; D008870:Microtubules / Q000187:drug effects; D009040:Motion Pictures; D010053:Ovary / Q000166:cytology / Q000187:drug effects*; D010784:Photomicrography; D013739:Testosterone / Q000494:pharmacology*; D014747:Vinblastine / Q000494:pharmacology
D001688:Biological Products; D002087:Butyrates; D007211:Indoles; D013739:Testosterone; D014747:Vinblastine; D000242:Cyclic AMP; D003703:Demecolcine