title: DNA nucleotide sequence restricted by the RI endonuclease.
abstract: The sequence of DNA base pairs adjacent to the phosphodiester bonds cleaved by the RI restriction endonuclease in unmodified DNA from coliphage lambda has been determined. The 5'-terminal nucleotide labeled with (32)P and oligonucleotides up to the heptamer were analyzed from a pancreatic DNase digest. The following sequence of nucleotides adjacent to the RI break made in lambda DNA was deduced from these data and from the 3'-dinucleotide sequence and nearest-neighbor analysis obtained from repair synthesis with the DNA polymerase of Rous sarcoma virus [Formula: see text] The RI endonuclease cleavage of the phosphodiester bonds (indicated by arrows) generates 5'-phosphoryls and short cohesive termini of four nucleotides, (p)A(p)A(p)T(p)T. The most striking feature of the sequence is its symmetry.
mesh_terms: D000227:Adenine Nucleotides / Q000032:analysis; D000469:Alkaline Phosphatase / Q000378:metabolism; D001358:Avian Sarcoma Viruses / Q000201:enzymology; D001483:Base Sequence; D003090:Coliphages; D003597:Cytosine Nucleotides / Q000032:analysis; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism; D004352:Drug Resistance, Microbial; D004720:Endonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D005111:Extrachromosomal Inheritance; D009711:Nucleotides / Q000032:analysis* / Q000378:metabolism; D009841:Oligonucleotides / Q000032:analysis; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D010759:Phosphorus Isotopes; D011119:Polynucleotides / Q000032:analysis; D013698:Templates, Genetic; D013942:Thymine Nucleotides / Q000032:analysis
chemical_list: D000227:Adenine Nucleotides; D003597:Cytosine Nucleotides; D004279:DNA, Viral; D009711:Nucleotides; D009841:Oligonucleotides; D010759:Phosphorus Isotopes; D011119:Polynucleotides; D013942:Thymine Nucleotides; D004254:DNA Nucleotidyltransferases; D003851:Deoxyribonucleases; D004720:Endonucleases; D000469:Alkaline Phosphatase; D010727:Phosphoric Diester Hydrolases