Phosphorylation of ribosome-associated protein by an adenosine 3':5'-cyclic monophosphate-dependent protein kinase: location of the microsomal receptor and protein kinase.
In the adrenal cortex, the adenosine 3':5'-cyclic monophosphate (cAMP)-receptor protein and the cAMP-dependent protein kinase are located in both the cytosol and endoplasmic reticulum. The cAMP-dependent protein kinase from the cytosol catalyzes the phosphorylation of ribosome-associated protein. Ribosomes washed in 0.5 M NH(4)Cl retain the substrate of the protein kinase reaction, but are dependent on the NH(4)Cl extract for in vitro protein synthesis. Dissociation of the 80S ribosomes by 0.88 M KCl, however, releases the ribosome-associated (protein) substrate of the phosphorylation reaction.
D000227:Adenine Nucleotides / Q000378:metabolism*; D000311:Adrenal Glands / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D000643:Ammonium Chloride; D000818:Animals; D002417:Cattle; D000242:Cyclic AMP / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000201:enzymology / Q000378:metabolism*; D010759:Phosphorus Isotopes; D010770:Phosphotransferases / Q000378:metabolism*; D014176:Protein Biosynthesis; D011955:Receptors, Drug; D012270:Ribosomes / Q000378:metabolism*
D000227:Adenine Nucleotides; D010759:Phosphorus Isotopes; D011955:Receptors, Drug; D000643:Ammonium Chloride; D000242:Cyclic AMP; D010770:Phosphotransferases