Genetics of somatic mammalian cells: lethal antigens as genetic markers for study of human linkage groups.
The antigen that causes killing of at least 98% of a human cell population treated with a 1% solution of a specific rabbit antiserum in the presence of complement is a sensitive genetic marker. The rapid loss of human chromosomes in human-Chinese hamster cell hybrids makes possible a convenient test of linkage relationships with this marker. Hybrid clones with and without the lethal antigen were isolated and analyzed. In 76 clones and subclones studied, 41 carried both the lethal antigen and the lactic dehydrogenase-A marker, 35 carried neither, and no clones contained only one of the two markers. In contrast to this clear demonstration of linkage, absence of linkage was found between the lethal antigen and the following markers: Lactic dehydrogenase B, NAD-dependent malic dehydrogenase, NADP-dependent malic dehydrogenase, glucose-6-phosphate dehydrogenase, phosphoglucomutase, glutamate oxaloacetate transaminase, indophenol oxidase, glucose phosphate isomerase, proline, inositol, hypoxanthine B, and glycine A. This lethal antigen appears to be carried on a single human autosome.
D000818:Animals; D000941:Antigens; D001665:Binding Sites; D002459:Cell Fusion; D002874:Chromosome Mapping; D002999:Clone Cells; D003165:Complement System Proteins; D006224:Cricetinae; D003582:Cytogenetics; D005804:Genes, Lethal; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells; D006824:Hybridization, Genetic; D007106:Immune Sera; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D009154:Mutation; D010222:Parainfluenza Virus 1, Human / Q000528:radiation effects; D011830:Radiation Effects; D013045:Species Specificity; D014466:Ultraviolet Rays
D000941:Antigens; D007106:Immune Sera; D007527:Isoenzymes; D003165:Complement System Proteins; D007770:L-Lactate Dehydrogenase