Solubilization of envelopes of HVJ (Sendai virus) with alkali-emasol treatment and reassembly of envelope particles with removal of the detergent.
The envelopes of HVJ (Sendai virus) virions were solubilized with alkali-Emasol treatment. The solubilized envelope subunit(s) associated with hemagglutination-inhibiting antibody blocking, neuraminidase, and low hemagglutinating (HA) activities had a sedimentation coefficient of 8.8S. Envelope fragment-like structures were assembled from the solubilized subunits after Emasol was removed by gel filtration. These reassembled envelope particles with HA activity had cell-fusion activity as well as hemolytic activity. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the reassembled particles revealed that they mainly consisted of two kinds of polypeptides.
D000468:Alkalies / Q000494:pharmacology; D000818:Animals; D002459:Cell Fusion; D002499:Centrifugation, Density Gradient; D002501:Centrifugation, Zonal; D002642:Chick Embryo; D002850:Chromatography, Gel; D004589:Electrophoresis, Disc; D006385:Hemagglutination Inhibition Tests; D006387:Hemagglutination, Viral; D006389:Hemagglutinins, Viral / Q000032:analysis; D006461:Hemolysis; D008854:Microscopy, Electron; D009439:Neuraminidase / Q000032:analysis; D010222:Parainfluenza Virus 1, Human / Q000032:analysis* / Q000187:drug effects; D010455:Peptides / Q000032:analysis; D013501:Surface-Active Agents / Q000494:pharmacology; D013997:Time Factors; D014764:Viral Proteins / Q000302:isolation & purification*
D000468:Alkalies; D006389:Hemagglutinins, Viral; D010455:Peptides; D013501:Surface-Active Agents; D014764:Viral Proteins; D009439:Neuraminidase