Abnormal membrane sodium transport in Liddle's syndrome.
We have documented the presence of abnormal sodium transport in Liddle's syndrome by measuring sodium concentration, sodium influx, and fractional sodium outflux in vitro in erythrocytes from normal subjects, two patients with Liddle's syndrome, and one patient with primary hyperaldosteronism. Sodium influx and fractional sodium outflux, but not sodium concentration, were significantly increased in patients with Liddle's syndrome. Sodium outflux in a patient with primary hyperaldosteronism did not differ significantly from normal. These alterations of sodium transport in erythrocytes from patients with Liddle's syndrome were not attributable to circulating levels of aldosterone, renin, angiotensin, or serum potassium. Furthermore, changes in aldosterone secretory rate and levels of circulating renin produced by varying dietary sodium intake, did not alter sodium influx or fractional sodium outflux in either patients with Liddle's syndrome or normal subjects. The response of fractional sodium outflux and sodium influx to ouabain, ethacrynic acid, and to changes in the cation composition of the incubation medium suggests that the increased sodium fluxes in Liddle's syndrome do not result solely from a quantitative increase in those components of sodium transport which occur in normal human erythrocytes. Instead, at least a portion of the increased erythrocyte sodium transport in Liddle's syndrome represents a component of sodium transport which does not occur in normal human erythrocytes.
D000293:Adolescent; D000328:Adult; D000450:Aldosterone / Q000378:metabolism; D000804:Angiotensin II / Q000378:metabolism; D001692:Biological Transport; D002462:Cell Membrane / Q000503:physiopathology*; D002648:Child; D004912:Erythrocytes / Q000378:metabolism*; D004976:Ethacrynic Acid / Q000494:pharmacology*; D005260:Female; D006801:Humans; D006973:Hypertension; D007008:Hypokalemia; D066298:In Vitro Techniques; D008297:Male; D009994:Osmolar Concentration; D010042:Ouabain / Q000494:pharmacology*; D011188:Potassium / Q000097:blood / Q000378:metabolism; D015499:Renal Tubular Transport, Inborn Errors / Q000097:blood / Q000378:metabolism*; D012083:Renin / Q000378:metabolism; D012964:Sodium / Q000097:blood / Q000378:metabolism*; D012975:Sodium Isotopes
D012975:Sodium Isotopes; D000804:Angiotensin II; D000450:Aldosterone; D010042:Ouabain; D012964:Sodium; D012083:Renin; D004976:Ethacrynic Acid; D011188:Potassium