Size, composition, and structure of the deoxyribonucleic acid of herpes simplex virus subtypes 1 and 2.
Studies of the size, composition, and structure of the deoxyribonucleic acid (DNA) of the F and G prototypes of herpes simplex virus (HSV) subtypes 1 and 2 (HSV-1 and HSV-2) showed the following. (i) As previously reported by Good-heart et al. HSV-1 and HSV-2 DNA have a buoyant density of 1.726 and 1.728 g/cm(3), corresponding to 67 and 69 guanine +/- cytosine moles per cent, respectively. The difference in guanine plus cytosine content of the DNA species was confirmed by the finding of a 1 C difference in T(m). (ii) The DNA from purified virus on cocentrifugation with T4 DNA in neutral sucrose density gradients sedimented at 55S, corresponding to 99 +/- 5 million daltons in molecular weight. HSV-1 and HSV-2 DNA could not be differentiated with respect to size. (iii) Cosedimentation of alkali-denatured DNA from purified virus with T4 DNA on alkaline sucrose density gradients consistently yielded several bands of single-stranded HSV DNA ranging from fragments 7 x 10(6) daltons to intact strands 48 x 10(6) daltons in molecular weight.
D000818:Animals; D002247:Carbon Isotopes; D002277:Carcinoma; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D003596:Cytosine / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D006147:Guanine / Q000032:analysis; D000882:Haplorhini; D006358:Hot Temperature; D006801:Humans; D007668:Kidney; D007822:Laryngeal Neoplasms; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D012703:Serotyping; D018139:Simplexvirus / Q000032:analysis* / Q000254:growth & development / Q000302:isolation & purification; D013395:Sucrose; D013936:Thymidine; D014316:Tritium; D014466:Ultraviolet Rays; D014529:Uridine
D002247:Carbon Isotopes; D002712:Chlorides; D004279:DNA, Viral; D014316:Tritium; D002586:Cesium; D013395:Sucrose; D006147:Guanine; D003596:Cytosine; D013936:Thymidine; D014529:Uridine