title: Evidence against the presence of 3',5'-cyclic adenosine monophosphate and relevant enzymes in Lactobacillus plantarum.
abstract: Analysis of cells of Lactobacillus plantarum, starved or undergoing induction, showed no 3', 5'-cyclic adenosine monophosphate (cAMP). Neither adenyl cyclase nor 3', 5'-cAMP phosphodiesterase was detected in extracts. Extracts of L. plantarum did not inhibit these two enzymes of Escherichia coli K-12, strain W1435. Incubation of adenosine triphosphate (ATP)-U-(14)C with cells or various cell-free fractions of L. plantarum did not produce labeled 3', 5'-cAMP. Of various 3', 5'-cyclic and acyclic nucleotides tested, only 3', 5'-cAMP, ATP, and yeast adenylic acid stimulated l-arabinose isomerase. Yeast adenylic acid was two to four times as effective as 3', 5'-cAMP or ATP. 2', 3'-cAMP was not effective.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000032:analysis* / Q000378:metabolism; D001089:Arabinose; D002247:Carbon Isotopes; D002474:Cell-Free System; D002854:Chromatography, Paper; D000242:Cyclic AMP / Q000032:analysis* / Q000096:biosynthesis; D004790:Enzyme Induction; D007535:Isomerases / Q000096:biosynthesis; D007778:Lactobacillus / Q000032:analysis* / Q000201:enzymology / Q000378:metabolism; D009587:Nitrogen Isotopes; D009711:Nucleotides / Q000378:metabolism; D010103:Oxygen Isotopes; D010727:Phosphoric Diester Hydrolases / Q000032:analysis* / Q000378:metabolism
chemical_list: D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D009711:Nucleotides; D010103:Oxygen Isotopes; D000255:Adenosine Triphosphate; D001089:Arabinose; D000242:Cyclic AMP; D010727:Phosphoric Diester Hydrolases; D000262:Adenylyl Cyclases; D007535:Isomerases