Cytoplasmic compartmentalization of the protein and ribonucleic acid species of vesicular stomatitis virus.
The cytoplasmic sites of synthesis in L cells of the protein and ribonucleic acid species of vesicular stomatitis virus were studied by polyacrylamide gel electrophoresis after fractionation of membrane and other cytoplasmic components by the Caliguiri-Tamm technique. The viral spike protein (glycoprotein G) was found primarily associated with a smooth membrane fraction which is rich in plasma membrane; the G protein was also present in fractions containing rough endoplasmic reticulum. The nonglycosylated envelope protein S (also called M) was found in the smooth membrane fractions but was more abundant in endoplasmic reticulum-enriched fractions. Longer labeling resulted in detection of nucleoprotein N, as well as other minor nucleocapsid proteins L and NS(1), in the cellular membrane fractions. The N protein appeared to be made in membrane-free cytoplasm along with progeny ribonucleic acid and later became associated with membrane containing G and S viral proteins.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002247:Carbon Isotopes; D002458:Cell Fractionation; D002462:Cell Membrane / Q000032:analysis; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D046508:Culture Techniques; D003593:Cytoplasm / Q000032:analysis / Q000382:microbiology*; D004589:Electrophoresis, Disc; D004721:Endoplasmic Reticulum / Q000032:analysis; D005944:Glucosamine / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis; D007739:L Cells; D008854:Microscopy, Electron; D009698:Nucleoproteins / Q000032:analysis; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000032:analysis* / Q000096:biosynthesis; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D014721:Vesicular stomatitis Indiana virus / Q000032:analysis* / Q000254:growth & development; D014764:Viral Proteins / Q000032:analysis* / Q000096:biosynthesis
D000596:Amino Acids; D002247:Carbon Isotopes; D006023:Glycoproteins; D009698:Nucleoproteins; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium; D005944:Glucosamine; D014529:Uridine