title: Deoxyribonucleic acid repair in bacteriophage T4: observations on the roles of the x and v genes and of host factors.
abstract: Studies were carried out to determine the effect of mutation in the host pol I gene on survival of ultraviolet (UV)-irradiated bacteriophage T4. Whereas a slightly reduced survival was observed in Escherichia coli strain P-3478 (pol A(1)) compared to strain W-3110 (pol A(+)), no such difference was observed in two strains isogenic except for the pol A gene. It was also shown that, whereas bacteriophage T4x is sensitive to UV irradiation, X irradiation, and treatment with methyl-methanesulfonate (MMS), phage T4v(1) is sensitive only to UV irradiation. The survival of damaged phage T4x is neither affected by the presence of the rec A, rec B, or pol A mutations in the host, nor is there evidence that phage T4 effects repair of rec A or pol A mutants previously treated with either UV or MMS.
mesh_terms: D000476:Alkanesulfonates / Q000494:pharmacology; D002470:Cell Survival; D002474:Cell-Free System; D003090:Coliphages / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D004260:DNA Repair; D004269:DNA, Bacterial / Q000096:biosynthesis; D004279:DNA, Viral / Q000096:biosynthesis*; D003851:Deoxyribonucleases / Q000378:metabolism; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism* / Q000528:radiation effects; D005796:Genes; D009154:Mutation; D011830:Radiation Effects; D011995:Recombination, Genetic; D014466:Ultraviolet Rays
chemical_list: D000476:Alkanesulfonates; D004269:DNA, Bacterial; D004279:DNA, Viral; D004254:DNA Nucleotidyltransferases; D003851:Deoxyribonucleases