Quantitative characteristics of the transformation of hamster cells by PARA (defective simian virus 40)-adenovirus 7.
An in vitro method for the quantitative measurement of transformation in hamster embryo fibroblasts by the PARA [defective simian virus 40 (SV40)]-adenovirus 7 hybrid has been developed. Transformation by PARA particles followed one-hit kinetics with a ratio of 1 focus-forming unit per 250 plaque-forming units. The method of viral adsorption had a direct effect upon the total number of foci which developed but not on the quantitative aspects of this assay. A fluorescent-focus assay was developed which provided a direct correlation of the observed morphological transformation and the presence of the PARA genome. This fluorescent-focus assay utilized detection of the SV40 tumor antigen, which was present in all foci transformed by PARA. Single foci induced by PARA were isolated and grown into cell lines. Two types of foci were observed and isolated; the first contained cells having a cuboidal or SV40-type morphology, and the second consisted of epithelial or adenovirus-type transformed cells. Both types contained the SV40 tumor and SV40 surface antigens as determined by the indirect fluorescence technique; however, only the epithelial cells contained the adenovirus 7 tumor antigen. All five cell lines which were injected into weanling Syrian hamsters were found to be oncogenic. These cell lines induced antibodies to both SV40 and adenovirus 7 tumor antigens in tumor-bearing animals.
D000256:Adenoviridae / Q000276:immunology / Q000472:pathogenicity*; D000327:Adsorption; D000818:Animals; D000941:Antigens / Q000032:analysis; D002471:Cell Transformation, Neoplastic; D006224:Cricetinae; D046508:Culture Techniques; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D005827:Genetics, Microbial; D006824:Hybridization, Genetic; D008856:Microscopy, Fluorescence; D013539:Simian virus 40 / Q000276:immunology / Q000472:pathogenicity*
D000941:Antigens