title: Lactate dehydrogenase isozymes: further kinetic studies at high enzyme concentration.
abstract: By competition with lactate dehydrogenase (LDH) for nicotinamide adenine dinucleotide (NAD), commonly occurring intracellular proteins, such as glyceraldehyde-3-phosphate dehydrogenase, malate dehydrogenase, and albumin, can protect LDH-1 and LDH-5 from inhibition and ternary complex formation with NAD and pyruvate. The existence of intracellular proteins that compete with LDH for NAD renders unphysiological a model for estimating the extent of intracellular LDH inhibition based on incubations of only LDH, NAD, and pyruvate.
mesh_terms: D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D007527:Isoenzymes; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors*; D008291:Malate Dehydrogenase; D008956:Models, Chemical; D009243:NAD; D011773:Pyruvates; D012710:Serum Albumin, Bovine
chemical_list: D007527:Isoenzymes; D011773:Pyruvates; D009243:NAD; D012710:Serum Albumin, Bovine; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases