Patterns of Simian Virus 40 DNA transcription after acute infection of permissive and nonpermissive cells.
Small amounts of fractionated, denatured, (32)P-labeled DNA from SV40 virus were incubated with a large excess of the complementary RNA of SV40 prepared in vitro with Escherichia coli RNA polymerase; the viral DNA strands were separated on hydroxyapatite columns. The RNA present in green monkey cells late in the lytic cycle reacted with 40-42% of the strand complementary to the in vitro complementary RNA (minus strand), and 60-64% of the opposite (plus) strand. "Early lytic" RNA failed to significantly interact with the plus strand, but formed stable duplex molecules with 35-39% of the minus strand. The RNA prepared from mouse embryo cells 24 hr after infection with SV40 combined with 35-38% of the minus strand and 60-62% of the plus strand. In all cases, the same regions of either the plus or minus strand appear to be transcribed in permissive and nonpermissive infections.
D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002845:Chromatography; D003561:Cytarabine / Q000494:pharmacology; D004279:DNA, Viral / Q000096:biosynthesis / Q000302:isolation & purification; D004622:Embryo, Mammalian / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D000882:Haplorhini; D006882:Hydroxyapatites; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D010710:Phosphates / Q000378:metabolism; D010759:Phosphorus Isotopes; D012316:RNA Nucleotidyltransferases; D012367:RNA, Viral / Q000096:biosynthesis*; D013539:Simian virus 40; D013698:Templates, Genetic
D004279:DNA, Viral; D006882:Hydroxyapatites; D010710:Phosphates; D010759:Phosphorus Isotopes; D012367:RNA, Viral; D003561:Cytarabine; D012316:RNA Nucleotidyltransferases