Effect of environmental pH on the efficiency of cellular hybridization.
The hybridization of a human and mouse cell was strikingly pH-dependent, with a well defined optimum at (about) pH 7.6-8.0. The yield of hybrid cell colonies (1 per 500-2000 heterokaryocytes) was several hundred times greater than that obtained at pH 6.8-7.2. Although there was a significant effect on the efficiency of cell fusion, the critical time for the pH effect was in the first 4-8 days after fusion, presumably while viable hybrids were being formed from the multinucleated heterokaryocytes.
D000818:Animals; D001345:Autoradiography; D002459:Cell Fusion; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured / Q000187:drug effects; D006801:Humans; D006822:Hybrid Cells; D006824:Hybridization, Genetic; D006863:Hydrogen-Ion Concentration; D007739:L Cells / Q000187:drug effects; D008244:Lysophosphatidylcholines / Q000494:pharmacology; D051379:Mice; D010222:Parainfluenza Virus 1, Human; D013997:Time Factors
D008244:Lysophosphatidylcholines