A polymer of glucose and N-acetylgalactosamine 1-phosphate in the wall of Micrococcus sp. A1.
1. The walls of Micrococcus sp. A1contain about 43% of a phosphorylated polymer. It was extracted with cold trichloroacetic acid and purified by chromatography on DEAE-cellulose. 2. The polymer contained equimolar amounts of d-glucose, N-acetylgalactosamine and phosphate, and was readily hydrolysed under gentle acidic conditions to a phosphorylated disaccharide. 3. Chemical and enzymic degradation indicated that this was 3-O-alpha-d-glucopyranosyl-N-acetylgalactosamine with a phosphomonoester group at the 6-position on the glucose. 4. Related degradation of the polymer itself indicated that the repeating structure was the disaccharide with a phosphodiester residue joining the 1-position on galactosamine to the 6-position on glucose in a neighbouring unit. This polymer is thus another example of the increasing number of microbial wall polymers or teichoic acids possessing sugar 1-phosphate linkages.
D001894:Borohydrides; D002473:Cell Wall / Q000032:analysis; D002848:Chromatography, DEAE-Cellulose; D002854:Chromatography, Paper; D005561:Formates; D005688:Galactosamine / Q000032:analysis; D005947:Glucose / Q000032:analysis*; D006595:Hexosamines / Q000032:analysis*; D006851:Hydrochloric Acid; D006868:Hydrolysis; D046911:Macromolecular Substances; D008837:Micrococcus / Q000032:analysis*; D010428:Pentosephosphates / Q000032:analysis; D010504:Periodic Acid; D010710:Phosphates / Q000032:analysis*; D011135:Polysaccharides, Bacterial / Q000032:analysis
D001894:Borohydrides; D005561:Formates; D006595:Hexosamines; D046911:Macromolecular Substances; D010428:Pentosephosphates; D010710:Phosphates; D011135:Polysaccharides, Bacterial; D010504:Periodic Acid; D005688:Galactosamine; D005947:Glucose; D006851:Hydrochloric Acid