System of double infection between vaccinia virus and mengovirus.
When L cells are simultaneously infected with vaccinia virus and mengovirus, double interference in the replication of both viruses is observed. Superinfection of vaccinia virus-infected cells by mengovirus during the first 5 hr of infection reduces vaccinia virus yields to between 1 and 3% of controls. The yields of mengovirus are reduced to between 1 and 16% of controls, depending upon the time of superinfection. The replication of vaccinia deoxyribonucleic acid is not inhibited by mengovirus; it is only delayed. On the other hand, vaccinia multiplication severely hinders the replication of mengovirus ribonucleic acid. The double-infected system, at early times, synthesizes proteins that resemble those synthesized in the vaccinia virus-infected cells. Later in infection, however, the pattern is switched to proteins synthesized by mengovirus-infected cells. Possible mechanisms for this double interference in multiplication are discussed.
D000818:Animals; D002247:Carbon Isotopes; D002642:Chick Embryo; D046508:Culture Techniques; D003593:Cytoplasm / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis; D004589:Electrophoresis, Disc; D004686:Encephalomyocarditis virus / Q000254:growth & development* / Q000378:metabolism / Q000472:pathogenicity; D005347:Fibroblasts; D007739:L Cells / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D010649:Phenylalanine / Q000378:metabolism; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D014616:Vaccinia virus / Q000254:growth & development* / Q000378:metabolism / Q000472:pathogenicity; D014633:Valine / Q000378:metabolism; D014762:Viral Interference; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
D002247:Carbon Isotopes; D004279:DNA, Viral; D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium; D010649:Phenylalanine; D012313:RNA; D004247:DNA; D007930:Leucine; D014633:Valine; D013936:Thymidine; D014529:Uridine