Contact-inhibited revertant cell lines isolated from simian virus 40-transformed cells. 3. Concanavalin A-selected revertant cells.
Contact-inhibited variants have been isolated by treatment of simian virus 40 (SV40)-transformed Balb/c 3T3 cells (SVT2) with the plant lectin concanavalin A. These con A revertant cells exhibit the following properties: (i) they resemble 3T3 cells morphologically and grow to saturation densities which are similar to that of 3T3 cells; (ii) they synthesize the SV40-specific T antigen and yield infectious virus after fusion with permissive monkey cells; (iii) they contain a high sialic acid content similar to that of 3T3 cells and not to that of SVT2 cells; sialic acid composition was found to be independent of serum concentration; (iv) they contain more chromosomes with the average number in the tetraploid range than the SVT2 cells from which they were derived; and (v) SVT2 and revertant cells, confluent or subconfluent, produce more collagen than Balb/3T3 cells. The relationship of surface membrane properties to contact inhibition of growth and the mechanisms for generating revertant cells are discussed.
D000371:Agglutination; D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification; D002459:Cell Fusion; D002460:Cell Line / Q000032:analysis / Q000187:drug effects* / Q000276:immunology; D002471:Cell Transformation, Neoplastic; D002875:Chromosomes; D002999:Clone Cells; D003094:Collagen / Q000032:analysis; D003208:Concanavalin A / Q000494:pharmacology; D003260:Contact Inhibition; D005467:Floxuridine / Q000494:pharmacology; D014644:Genetic Variation; D000882:Haplorhini; D037102:Lectins / Q000494:pharmacology*; D051379:Mice; D009438:Neuraminic Acids / Q000032:analysis; D011123:Polyploidy; D011392:Proline; D013539:Simian virus 40 / Q000276:immunology / Q000302:isolation & purification; D014316:Tritium
D000956:Antigens, Viral; D037102:Lectins; D009438:Neuraminic Acids; D005467:Floxuridine; D014316:Tritium; D003208:Concanavalin A; D003094:Collagen; D011392:Proline