Adenovirus type 2-simian virus 40 hybrid population: evidence for a hybrid deoxyribonucleic acid molecule and the absence of adenovirus-encapsidated circular simian virus 40 deoxyribonucleic acid.
The deoxyribonucleic acid (DNA) from the adenovirus-encapsidated particles of the adenovirus type 2 (Ad2)-simian virus 40 (SV40) hybrid population plaque variant (Ad2(++) HEY), known to yield SV40 virus with high efficiency, was studied by equilibrium density centrifugation followed by ribonucleic acid-DNA hybridization employing virus-specific complementary ribonucleic acids synthesized in vitro. These techniques establish linkage between the Ad2 and SV40 components in the adenovirus-encapsidated particles of this population. The linkage is alkali-resistant and presumably covalent; thus, the Ad2 DNA and SV40 DNA are present in a hybrid molecule. Velocity centrifugation studies in alkaline sucrose gradients eliminated the possibility that supercoiled circular SV40 DNA is present in the adenovirus capsids. The DNA obtained from the adenovirus-encapsidated particles of the Ad2(++) HEY population appears to consist of nonhybrid Ad2 DNA and Ad2-SV40 hybrid DNA molecules.
D000256:Adenoviridae / Q000032:analysis* / Q000254:growth & development; D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D004279:DNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D014644:Genetic Variation; D005827:Genetics, Microbial; D000882:Haplorhini; D006801:Humans; D006824:Hybridization, Genetic; D007668:Kidney; D008967:Molecular Biology; D009691:Nucleic Acid Denaturation; D010759:Phosphorus Isotopes; D012367:RNA, Viral; D013539:Simian virus 40 / Q000032:analysis* / Q000254:growth & development; D013395:Sucrose; D013936:Thymidine; D014316:Tritium
D002712:Chlorides; D004279:DNA, Viral; D010759:Phosphorus Isotopes; D012367:RNA, Viral; D014316:Tritium; D002586:Cesium; D013395:Sucrose; D013936:Thymidine