Separation of the infectious ribonucleic acid of potato spindle tuber virus from double-stranded ribonucleic acid of plant tissue extracts.
The infectious ribonucleic acid (RNA) of potato spindle tuber virus (PSTV) can be separated by hydroxyapatite chromatography from double-stranded RNA detectable in low amounts in both infected and uninfected plant tissue extracts. The chromatographic behavior of ribonuclease-sensitive PSTV RNA resembles that of transfer RNA.
D000818:Animals; D002845:Chromatography; D046508:Culture Techniques; D003851:Deoxyribonucleases; D006882:Hydroxyapatites; D007668:Kidney; D008722:Methods; D010935:Plant Diseases; D010936:Plant Extracts; D010942:Plant Viruses / Q000032:analysis / Q000472:pathogenicity; D010945:Plants, Edible; D012313:RNA / Q000302:isolation & purification*; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D011817:Rabbits; D012260:Ribonucleases; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development; D014762:Viral Interference
D006882:Hydroxyapatites; D010936:Plant Extracts; D012367:RNA, Viral; D012313:RNA; D003851:Deoxyribonucleases; D012260:Ribonucleases