Electron spin resonance studies of carbonic anhydrase: transition metal ions and spin-labeled sulfonamides.
Electron spin resonance (esr) spectra of Cu(II) and Co(II) carbonic anhydrase, and a spin-labeled sulfonamide complex of the Zn(II) enzyme, are reported. The coordination geometry of Cu(II) bound in the enzyme appears to have approximately axial symmetry. Esr spectra of enzyme complexes with metal-binding anions also show axial symmetry and greater covalency, in the order ethoxzolamide < SH(-) < N(3) (-) </= CN(-). Well-resolved superhyperfine structure in the spectrum of the cyanide complex suggests the presence of two, and probably three, equivalent nitrogen ligands from the protein. Esr spectra of the Co(II) enzyme and its complexes show two types of Co(II) environment, one typical of the native enzyme and the 1:1 CN(-) complex, and one typical of a 2:1 CN(-) complex. Co(II) in the 2:1 complex appears to be low-spin and probably has a coordination number of 5. Binding of a spin-labeled sulfonamide to the active center immobilizes the free radical. The similarity of the esr spectra of spin-labeled Zn(II) and Co(II) carbonic anhydrases suggests that the conformation at the active center is similar in the two metal derivatives.
D001665:Binding Sites; D002256:Carbonic Anhydrases; D003035:Cobalt; D003300:Copper; D003486:Cyanides; D004578:Electron Spin Resonance Spectroscopy; D013440:Sulfides; D013449:Sulfonamides; D015032:Zinc
D003486:Cyanides; D013440:Sulfides; D013449:Sulfonamides; D003035:Cobalt; D003300:Copper; D002256:Carbonic Anhydrases; D015032:Zinc