2'-O-methylation of adenosine, guanosine, uridine, and cytidine in RNA of isolated rat liver nuclei.
Nuclei isolated from rat liver, when incubated with methyl-labeled S-adenosylmethionine, incorporated label into 2'-O-methyladenosine, 2'-O-methylguanosine, 2'-O-methyluridine, and 2'-O-methylcytidine of endogenous nuclear RNA. Addition of ribosomal RNA from wheat germ to the reaction markedly stimulated 2'-O-methylation of total RNA. The relative incorporation of label into the four different 2'-O-methyl ribonucleosides was greatly changed by the addition of wheat germ RNA. There was much more 2'-O-methylation of the purine ribonucleosides, relative to the pyrimidine ribonucleosides, in the reaction stimulated by wheat germ RNA.
D000227:Adenine Nucleotides / Q000378:metabolism; D000469:Alkaline Phosphatase; D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D002848:Chromatography, DEAE-Cellulose; D002854:Chromatography, Paper; D003597:Cytosine Nucleotides / Q000378:metabolism; D006150:Guanine Nucleotides / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008738:Methyl Ethers / Q000096:biosynthesis; D008745:Methylation; D010727:Phosphoric Diester Hydrolases; D010944:Plants; D011119:Polynucleotides / Q000494:pharmacology; D012313:RNA / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000494:pharmacology; D012343:RNA, Transfer / Q000494:pharmacology; D051381:Rats; D012436:S-Adenosylmethionine / Q000378:metabolism; D014316:Tritium; D014500:Uracil Nucleotides / Q000378:metabolism
D000227:Adenine Nucleotides; D003597:Cytosine Nucleotides; D006150:Guanine Nucleotides; D008738:Methyl Ethers; D011119:Polynucleotides; D012335:RNA, Ribosomal; D014500:Uracil Nucleotides; D014316:Tritium; D012313:RNA; D012436:S-Adenosylmethionine; D012343:RNA, Transfer; D000469:Alkaline Phosphatase; D010727:Phosphoric Diester Hydrolases