Photo-affinity labels for adenosine 3':5'-cyclic monophosphate.
Three derivatives of cyclic AMP that are potentially useful as photo-affinity labels were synthesized. One of these derivatives is, upon photolysis, specifically incorporated into the cyclic AMP-binding site of rabbit muscle phosphofructokinase. The use of these derivatives in isolating and identifying cyclic AMP receptor sites is discussed.
D000818:Animals; D001391:Azo Compounds / Q000494:pharmacology; D001665:Binding Sites / Q000528:radiation effects; D055598:Chemical Phenomena; D002621:Chemistry; D002852:Chromatography, Ion Exchange; D000242:Cyclic AMP; D004789:Enzyme Activation; D008314:Malonates / Q000494:pharmacology; D009132:Muscles / Q000201:enzymology; D010732:Phosphofructokinase-1 / Q000032:analysis; D010777:Photochemistry; D010782:Photolysis; D011817:Rabbits; D011830:Radiation Effects; D011955:Receptors, Drug; D014316:Tritium; D014466:Ultraviolet Rays
D001391:Azo Compounds; D008314:Malonates; D011955:Receptors, Drug; D014316:Tritium; D000242:Cyclic AMP; D010732:Phosphofructokinase-1