title: RNA-dependent RNA polymerase activity associated with virions and subviral particles of myxoviruses.
abstract: In vitro RNA synthesis is catalyzed by an enzyme found in purified virions of each of nine influenza strains tested. The extent of [(3)H]UTP incorporation observed is strain dependent, with an obligate requirement for Mn(2+) ions and RNA template. At least 30% of purified product RNA shows resistance to degradation by ribonuclease A and T1 before and after annealing with exogenous RNA from influenza virions.
mesh_terms: D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D066298:In Vitro Techniques; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D009522:Newcastle disease virus / Q000201:enzymology*; D009975:Orthomyxoviridae / Q000201:enzymology*; D012316:RNA Nucleotidyltransferases / Q000032:analysis; D012367:RNA, Viral / Q000096:biosynthesis; D012260:Ribonucleases / Q000494:pharmacology; D013045:Species Specificity; D013698:Templates, Genetic; D014316:Tritium; D014500:Uracil Nucleotides / Q000378:metabolism; D014721:Vesicular stomatitis Indiana virus / Q000201:enzymology
chemical_list: D012367:RNA, Viral; D014500:Uracil Nucleotides; D014316:Tritium; D008345:Manganese; D012316:RNA Nucleotidyltransferases; D012260:Ribonucleases; D008274:Magnesium