title: Fine structure of protoplasts and L-forms of Clostridium botulinum types A and E.
abstract: Spherical bodies, that are obtained by adding penicillin and lysozyme to Clostridium botulinum types E and A cultures which are growing in an osmotically stabilized medium, are shown to be protoplasts by electron microscopy. The L-forms of these two culture types have morphologically different inclusion bodies.
mesh_terms: D001431:Bacteriological Techniques; D003014:Clostridium botulinum / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development; D003470:Culture Media; D002479:Inclusion Bodies; D007740:L Forms / Q000166:cytology*; D008854:Microscopy, Electron; D009113:Muramidase / Q000494:pharmacology; D010400:Penicillin G / Q000494:pharmacology; D011523:Protoplasts / Q000166:cytology*
chemical_list: D003470:Culture Media; D009113:Muramidase; D010400:Penicillin G