Deoxyribonucleic acid polymerase associated with avian tumor viruses: secondary structure of the deoxyribonucleic acid product.
The products of the deoxyribonucleic acid (DNA) polymerase associated with Rous sarcoma virus and avian myeloblastosis virus were characterized by correlative analyses with equilibrium centrifugation and stepwise elution from hydroxyapatite. The initial enzymatic product consists of nascent DNA chains which are hydrogen-bonded to 70S viral ribonucleic acid (RNA), whereas the final enzymatic product is double-stranded DNA. Appreciable amounts of free single-stranded DNA were not detected at any point during the course of the enzymatic reaction, but the data in this regard are not decisive. The time course of synthesis of DNA:RNA hybrids and double-stranded DNA has been analyzed. It is concluded that the synthesis of double-stranded DNA is a sequel to and is probably dependent upon the synthesis of DNA:RNA hybrid.
D000818:Animals; D001354:Avian Leukosis Virus / Q000201:enzymology* / Q000378:metabolism; D002417:Cattle; D002499:Centrifugation, Density Gradient; D002501:Centrifugation, Zonal; D002586:Cesium; D002642:Chick Embryo; D002845:Chromatography; D004247:DNA / Q000032:analysis / Q000096:biosynthesis; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*
D002586:Cesium; D004247:DNA; D004254:DNA Nucleotidyltransferases