Asymmetric template function of microbial deoxyribonucleic acids: transcription of messenger ribonucleic acid.
In Bacillus subtilis and Escherichia coli, pulse-labeled ribonucleic acid (RNA) synthesized during step-down growth hybridized preferentially with the heavy (H) strand of methylated albumin-Kieselguhr-fractionated deoxyribonucleic acid (DNA). At high RNA inputs, the ratio of RNA hybridized with the H strand to that hybridized with the light (L) strand was 8.7 for B. subtilis and 2.0 for E. coli. At high DNA inputs, the H/L hybridization ratio increased by a factor of two. This change in the hybridization ratio was attributable to the fraction of the pulse-labeled RNA which is in stable RNA components. The hybridization peak of pulse-labeled RNA was specifically located in the late-eluting region of the absorbance profile of the H strand. This region was considered to represent the most actively transcribing H strand templates.
D001412:Bacillus subtilis / Q000378:metabolism; D002845:Chromatography; D004269:DNA, Bacterial / Q000302:isolation & purification; D004926:Escherichia coli / Q000378:metabolism; D005815:Genetic Code; D005827:Genetics, Microbial; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D012329:RNA, Bacterial / Q000096:biosynthesis; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide; D013698:Templates, Genetic; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D014316:Tritium; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide; D014529:Uridine