The biosynthesis of serine and glycine in Pseudomonas AM1 with special reference to growth on carbon sources other than C1 compounds.
1. A mutant, 20S, of Pseudomonas AM1 was obtained that requires a supplement of serine to grow on succinate, lactate or ethanol. This mutant lacks phosphoserine phosphatase and revertants to wild-type phenotype regained this enzymic activity showing that the phosphorylated pathway of serine biosynthesis is necessary for growth on these three substrates. 2. The requirement for supplemental serine by mutant 20S could be met by glycine, suggesting that Pseudomonas AM1 can obtain C(1) units from glycine. 3. Mutant 20S grows on C(1) compounds at a lower rate compared with the wild type. Supplementation with serine stimulated the growth rate of the mutant suggesting that the phosphorylated pathway of serine biosynthesis plays some role, but not an essential role, during growth on C(1) compounds. 4. A mutant, 82G, was obtained that requires a supplement of glycine to grow on succinate, lactate or ethanol. When grown in such supplemented media, the mutant lacks serine hydroxymethyltransferase and revertants to wild-type phenotype regained enzymic activity showing that during growth on succinate, lactate or ethanol, glycine is made from serine via serine hydroxymethyltransferase, and that the organism can obtain C(1) units from glycine. 5. Mutant 82G grew on methanol and then contained serine hydroxymethyltransferase suggesting that this enzyme is necessary for growth on C(1) compounds and that Pseudomonas AM1 may synthesize two such enzymes, one used in growth on C(1) compounds, the other used in growth on other substrates. Mutant 82G might lack the latter enzyme. 6. Phosphoglycerate dehydrogenase is specifically inhibited by l-serine and the regulatory implications of this are discussed.
D002244:Carbon / Q000378:metabolism*; D003470:Culture Media; D000431:Ethanol / Q000378:metabolism; D005998:Glycine / Q000096:biosynthesis* / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D000432:Methanol / Q000378:metabolism; D009154:Mutation; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D011549:Pseudomonas / Q000201:enzymology / Q000378:metabolism*; D012694:Serine / Q000096:biosynthesis*; D013386:Succinates / Q000378:metabolism; D014166:Transferases / Q000378:metabolism
D003470:Culture Media; D007773:Lactates; D013386:Succinates; D000431:Ethanol; D012694:Serine; D002244:Carbon; D014166:Transferases; D010744:Phosphoric Monoester Hydrolases; D005998:Glycine; D000432:Methanol