Effect of vesicular stomatitis virus infection on the histocompatibility antigen of L cells.
When mouse L cells are infected for 22 hr with vesicular stomatitis virus (VSV), a ribonucleic acid-containing enveloped virus, greater than 70% of the major histocompatibility antigen (H-2), is no longer detectable by the method of inhibition of immune cytolysis. Infected cells prelabeled with (14)C-glucosamine also show a correspondingly greater loss of trichloroacetic acid-insoluble radioactivity than uninfected cells. The loss of H-2 antigenic activity is not due to the viral inhibition of host cell protein synthesis since cells cultured for 18 hr in the presence of cycloheximide have the same amount of H-2 activity as untreated controls. Also, cells infected with encephalomyocarditis virus, a picornavirus, show no loss of H-2 activity at a time when host cell protein synthesis is completely inhibited. VSV structural proteins associated in vitro with uninfected L-cell plasma membranes do not render H-2 sites inaccessible to the assay. Although antibodies may not combine with all the H-2 antigenic sites on the plasma membrane, anti-H-2 serum reacted with L cells before infection does not prevent a normal infection with VSV. H-2 activity can be detected in virus samples purified from the medium of infected L cells; this virus purified after being mixed with L-cell homogenates shows greater H-2 activity than virus purified after being mixed with HeLa cell homogenates. However, VSV made in HeLa cells shows no H-2 activity when mixed with L-cell homogenates.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001666:Binding Sites, Antibody; D002247:Carbon Isotopes; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D002859:Chromium Isotopes; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004686:Encephalomyocarditis virus; D005944:Glucosamine / Q000378:metabolism; D006367:HeLa Cells / Q000276:immunology; D006649:Histocompatibility Antigens; D006801:Humans; D007739:L Cells / Q000276:immunology*; D051379:Mice; D012367:RNA, Viral / Q000096:biosynthesis; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology* / Q000378:metabolism; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000378:metabolism
D000596:Amino Acids; D002247:Carbon Isotopes; D002859:Chromium Isotopes; D006649:Histocompatibility Antigens; D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium; D003609:Dactinomycin; D003513:Cycloheximide; D005944:Glucosamine; D014529:Uridine