Ribose utilization by Veillonella alcalescens.
The utilization of ribose by Veillonella alcalescens has been further investigated. Nonfermentation of ribose is not a result of a phosphorylation lesion since ribose-phosphorylating activity was measured in cell extracts. Resting cells accumulated ribose-5-phosphate and nucleotides when (14)C-ribose was provided; no other sugar phosphates were detectable. Resting cells that were shifted to growth conditions polymerized rather than degraded the accumulated ribose compounds. Cell extracts contained a fructose diphosphate phosphatase. Ribose-5-phosphate, glucose-6-phosphate, and fructose-6-phosphate were not hydrolyzed. It is postulated that the nonfermentation of ribose is not due to any metabolic lesions, but is a consequence of metabolic control at the fructose diphosphate level of glycolysis.
D001345:Autoradiography; D002247:Carbon Isotopes; D002474:Cell-Free System; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D003470:Culture Media; D005285:Fermentation; D006597:Fructose-Bisphosphatase / Q000378:metabolism; D006019:Glycolysis; D009711:Nucleotides / Q000096:biosynthesis; D010085:Oxidative Phosphorylation; D010428:Pentosephosphates / Q000096:biosynthesis; D012266:Ribose / Q000378:metabolism*; D014678:Veillonella / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*
D002247:Carbon Isotopes; D003470:Culture Media; D009711:Nucleotides; D010428:Pentosephosphates; D012266:Ribose; D006597:Fructose-Bisphosphatase