title: Characterization of the low-molecular-weight RNAs associated with the 70S RNA of Rous sarcoma virus.
abstract: Two low-molecular-weight RNAs are associated with the 70S RNA complex of Rous sarcoma virus: a previously described 4S RNA and a newly identified 5S RNA. The 4S RNA constitutes 3 to 4% of the 70S RNA complex or the equivalent of 12 to 20 molecules per 70S RNA. It exhibits a number of structural properties characteristic of transfer RNA as revealed by two-dimensional electrophoresis of oligonucleotides obtained from a T1 ribonuclease digest of the 4S RNA species. The 5S RNA is approximately 120 nucleotides in length, constitutes 1% of the 70S RNA complex or the equivalent of 3 to 4 molecules per molecules of 70S RNA, and is identical in nucleotide composition and structure to 5S RNA from uninfected chicken embryo fibroblasts. Melting studies indicate that the 5S RNA is released from the 70S RNA complex at the same temperature required to dissociate 70S RNA into its constituent 35S subunits. In contrast, greater than 80% of the 4S RNA is released from 70S RNA prior to its conversion into subunits. The possible biological significance of these 70S-associated RNAs is discussed.
mesh_terms: D000241:Adenosine / Q000032:analysis; D000818:Animals; D001345:Autoradiography; D001358:Avian Sarcoma Viruses / Q000032:analysis* / Q000254:growth & development; D002642:Chick Embryo; D046508:Culture Techniques; D003562:Cytidine / Q000032:analysis; D004586:Electrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts; D006151:Guanosine / Q000032:analysis; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000032:analysis; D010761:Phosphorus Radioisotopes; D012367:RNA, Viral / Q000032:analysis*; D012260:Ribonucleases; D013696:Temperature; D014529:Uridine / Q000032:analysis
chemical_list: D009841:Oligonucleotides; D010761:Phosphorus Radioisotopes; D012367:RNA, Viral; D006151:Guanosine; D003562:Cytidine; D012260:Ribonucleases; D000241:Adenosine; D014529:Uridine