Nucleotide sequence analysis with polynucleotide kinase and nucleotide "mapping" methods. 5'-Terminal sequences of deoxyribonucleic acid from bacteriophages lambda and 424.
The polynucleotide kinase reaction was used in analyses of complex mixtures of oligodeoxynucleotides which were fractionated by various two-dimensional nucleotide ;mapping' procedures. Parallel ionophoretic analyses on DEAE-cellulose paper, pH2, and AE-cellulose paper, pH3.5, of venom phosphodiesterase partial digests of 5'-terminally labelled oligonucleotides enabled the sequence of the nucleotides to be deduced uniquely. A ;diagonal ionophoresis' method has been used with mixtures of nucleotides. Application of these methods to 5'-terminally labelled DNA from bacteriophage lambda gave the terminal sequences pA-G-G-T-C-G and pG-G-G-C-G. Identical 5'-terminal sequences were found with DNA from bacteriophage 424.
D000227:Adenine Nucleotides / Q000032:analysis; D000255:Adenosine Triphosphate; D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D003090:Coliphages / Q000032:analysis*; D003597:Cytosine Nucleotides / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis*; D003851:Deoxyribonucleases; D003854:Deoxyribonucleotides / Q000032:analysis*; D004590:Electrophoresis, Paper; D004720:Endonucleases; D004926:Escherichia coli / Q000201:enzymology; D006150:Guanine Nucleotides / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D009841:Oligonucleotides / Q000032:analysis; D010179:Pancreas / Q000201:enzymology; D010727:Phosphoric Diester Hydrolases; D010759:Phosphorus Isotopes; D010770:Phosphotransferases / Q000032:analysis*; D011119:Polynucleotides; D012911:Snakes; D013045:Species Specificity; D013942:Thymine Nucleotides / Q000032:analysis; D014688:Venoms
D000227:Adenine Nucleotides; D003597:Cytosine Nucleotides; D004279:DNA, Viral; D003854:Deoxyribonucleotides; D006150:Guanine Nucleotides; D009841:Oligonucleotides; D010759:Phosphorus Isotopes; D011119:Polynucleotides; D013942:Thymine Nucleotides; D014688:Venoms; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D003851:Deoxyribonucleases; D004720:Endonucleases; D010727:Phosphoric Diester Hydrolases