title: Transient inhibition of polyoma virus synthesis by sendai virus (parainfluenza I). II. Mechanism of the interference by inactivated virus.
abstract: The mechanism of the transient inhibition of polyoma virus synthesis by betapropiolactone-inactivated Sendai virus was studied. Polyoma virus early functions did not appear to be affected, although deoxyribonucleic acid (DNA) and structural protein synthesis were inhibited 60 and 35% respectively. The inhibition of macromolecular synthesis was not sufficient to account for the 90% inhibition of infectious progeny formation. Encapsidation of polyoma DNA into mature virions appears to be completely inhibited after superinfection by beta-propiolactone-inactivated Sendai virus. Ultraviolet irradiation of live or beta-propiolactone-inactivated Sendai virus preparations abolishes the interfering capacity, indicating that a functional Sendai virus ribonucleic acid molecule is the interfering component.
mesh_terms: D000818:Animals; D002471:Cell Transformation, Neoplastic; D002499:Centrifugation, Density Gradient; D046508:Culture Techniques; D004279:DNA, Viral / Q000096:biosynthesis; D003609:Dactinomycin / Q000494:pharmacology; D005467:Floxuridine / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006386:Hemagglutination Tests; D006571:Heterocyclic Compounds; D007783:Lactones / Q000494:pharmacology; D051379:Mice; D010222:Parainfluenza Virus 1, Human / Q000187:drug effects; D011120:Polyomavirus / Q000254:growth & development* / Q000378:metabolism; D011422:Propionates; D012367:RNA, Viral / Q000096:biosynthesis; D011830:Radiation Effects; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014466:Ultraviolet Rays; D014762:Viral Interference; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000096:biosynthesis
chemical_list: D004279:DNA, Viral; D006571:Heterocyclic Compounds; D007783:Lactones; D011422:Propionates; D012367:RNA, Viral; D014764:Viral Proteins; D005467:Floxuridine; D014316:Tritium; D003609:Dactinomycin; D013936:Thymidine