In vitro activation of the transcription of araBAD operon by araC activator.
The transcription of araBAD operon requires araC activator and cyclic AMP. D-Fucose inhibits ara mRNA synthesis. Our results indicate that the positive control by araC activator is exerted at the level of transcription.
D001089:Arabinose / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D002874:Chromosome Mapping; D003090:Coliphages; D000242:Cyclic AMP / Q000494:pharmacology; D004279:DNA, Viral; D004926:Escherichia coli / Q000378:metabolism*; D005643:Fucose / Q000494:pharmacology; D008242:Lysogeny; D009693:Nucleic Acid Hybridization; D009876:Operon; D012329:RNA, Bacterial / Q000032:analysis / Q000096:biosynthesis; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D013698:Templates, Genetic; D014158:Transcription, Genetic / Q000187:drug effects*; D014161:Transduction, Genetic; D014316:Tritium
D001426:Bacterial Proteins; D004279:DNA, Viral; D012329:RNA, Bacterial; D012333:RNA, Messenger; D014316:Tritium; D005643:Fucose; D001089:Arabinose; D000242:Cyclic AMP