Complementary RNA species isolated from vesicular stomatitis (HR strain) defective virions.
The wild-type strain of vesicular stomatitis virus (VSV) contains in its complete virion (VSV-1, B particles) a minus strand RNA. The principle defective particle of the wild-type strain (VSV-111, T particles) contains a shorter minus strand, homologous to part of the VSV-1 genome. Neither virion contains any detectable complementary (plus) strand RNA. In contrast, a preparation of a heat-resistant (HR) strain of VSV containing defective virions was found to contain both plus (21%) and minus strand RNA, present in several distinct size classes. It was found that the RNA in the HR virion preparation was at least 94% single-stranded and principally (96%) in ribonucleoprotein complexes. On extraction the plus and minus strand RNA species partially annealed to give a population of double- and multistranded RNA species. A small amount of RNA polymerase activity was associated with the HR defective virus preparation.
D000241:Adenosine; D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D003562:Cytidine; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D003673:Defective Viruses / Q000032:analysis* / Q000201:enzymology / Q000254:growth & development; D004591:Electrophoresis, Polyacrylamide Gel; D007668:Kidney; D009698:Nucleoproteins / Q000032:analysis; D010759:Phosphorus Isotopes; D012367:RNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D012260:Ribonucleases; D014158:Transcription, Genetic; D014316:Tritium; D014721:Vesicular stomatitis Indiana virus / Q000032:analysis* / Q000201:enzymology / Q000254:growth & development; D014764:Viral Proteins / Q000032:analysis
D009698:Nucleoproteins; D010759:Phosphorus Isotopes; D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium; D003562:Cytidine; D012321:DNA-Directed RNA Polymerases; D012260:Ribonucleases; D000241:Adenosine