Antiviral activity of an ether-extracted nonviable preparation of Brucella abortus.
Extraction of living Brucella abortus (strain 456) with aqueous ether yielded a nonviable, insoluble residue (Bru-Pel). When injected into mice, Bru-Pel was an effective, nontoxic interferon stimulus. Mice pretreated with Bru-Pel were protected against challenge with otherwise lethal doses of Semliki Forest virus. Significant protection was afforded when Bru-Pel was given as many as 7 days before virus challenge. Evidence is presented dealing with the complex nature of Bru-Pel and with the possibility that the antiviral activity of Bru-Pel may be associated not only with the production of interferon, but with a general increase in the level of nonspecific resistance in animals.
D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage; D001431:Bacteriological Techniques; D001921:Brain; D002003:Brucella abortus / Q000276:immunology*; D046508:Culture Techniques; D004986:Ether; D005260:Female; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D007369:Interferon Inducers; D007372:Interferons / Q000096:biosynthesis; D007739:L Cells; D051379:Mice; D011135:Polysaccharides, Bacterial; D012672:Semliki forest virus / Q000276:immunology*; D014020:Tissue Extracts; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology; D010948:Viral Plaque Assay; D014776:Virus Cultivation
D000942:Antigens, Bacterial; D007369:Interferon Inducers; D011135:Polysaccharides, Bacterial; D014020:Tissue Extracts; D004986:Ether; D007372:Interferons