Regulation of maltodextrin phosphorylase synthesis in Escherichia coli by cyclic adenosine 3', 5'-monophosphate and glucose.
Cyclic adenosine 3', 5'-monophosphate (AMP) stimulates maltodextrin phosphorylase synthesis in Escherichia coli cells induced with maltose. A maximal effect occurs at 2 to 3 mM cyclic AMP. The action of cyclic AMP is specific, inasmuch as adenosine triphosphate, 3'-AMP, 5'-AMP, adenosine, and dibutyryl cyclic AMP are inactive. Glucose, alpha-methyl glucoside, 2-deoxyglucose, and pyridoxal 5'-phosphate repress maltodextrin phosphorylase synthesis. This repression is reversed by cyclic AMP. The action of cyclic AMP appears to be at the transcriptional level, since cyclic AMP fails to stimulate phosphorylase production in induced cells in which messenger ribonucleic acid synthesis has been arrested by rifampin or by inducer removal. The two other enzymes involved in the metabolism of maltose, amylomaltase and maltose permease, are also induced in this strain of E. coli and affected by glucose and cyclic AMP in a manner similar to phosphorylase.
D000241:Adenosine / Q000494:pharmacology; D000249:Adenosine Monophosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D003994:Bucladesine / Q000494:pharmacology; D002250:Carbon Radioisotopes; D002474:Cell-Free System; D000242:Cyclic AMP / Q000494:pharmacology*; D004790:Enzyme Induction; D004794:Enzyme Repression; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology*; D005964:Glucosyltransferases / Q000096:biosynthesis / Q000378:metabolism; D008320:Maltose / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000096:biosynthesis / Q000378:metabolism; D006005:Phosphorylases / Q000096:biosynthesis* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D012293:Rifampin / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects
D002250:Carbon Radioisotopes; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; D000249:Adenosine Monophosphate; D003994:Bucladesine; D008320:Maltose; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D005964:Glucosyltransferases; D006005:Phosphorylases; D005947:Glucose; D000241:Adenosine; D012293:Rifampin