title: Characterization of a cold-sensitive hisW mutant of Salmonella typhimurium.
abstract: A cold-sensitive mutant of Salmonella typhimurium LT2 that grows at 37 C but not at 20 C has altered repression regulation in at least two amino acid biosynthetic pathways (histidine and isoleucine). The lesion conferring cold sensitivity that is linked with hisW is recessive. Assays for the acceptance of some amino acids by transfer ribonucleic acid (tRNA) reveal a decreased ability of the mutant tRNA to accept arginine, phenylalanine, and histidine. A mutation in a gene for tRNA maturation is a likely possibility for the mutation producing these effects on growth, regulation, and amino acid acceptance.
mesh_terms: D000215:Acylation; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D001120:Arginine / Q000378:metabolism; D001221:Aspartate Carbamoyltransferase / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis; D002247:Carbon Isotopes; D002474:Cell-Free System; D002874:Chromosome Mapping; D003080:Cold Temperature; D005808:Genes, Recessive; D006639:Histidine / Q000096:biosynthesis*; D006836:Hydro-Lyases / Q000378:metabolism; D009154:Mutation; D009954:Ornithine Carbamoyltransferase / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D012329:RNA, Bacterial / Q000096:biosynthesis; D012343:RNA, Transfer / Q000096:biosynthesis; D012486:Salmonella typhimurium / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D014161:Transduction, Genetic; D014316:Tritium; D014367:Tryptophan Synthase / Q000378:metabolism; D014498:Uracil / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002247:Carbon Isotopes; D012329:RNA, Bacterial; D014316:Tritium; D006639:Histidine; D014498:Uracil; D012343:RNA, Transfer; D001120:Arginine; D000429:Alcohol Oxidoreductases; D001221:Aspartate Carbamoyltransferase; D009954:Ornithine Carbamoyltransferase; D010744:Phosphoric Monoester Hydrolases; D006836:Hydro-Lyases; D014367:Tryptophan Synthase; D000604:Amino Acyl-tRNA Synthetases