Serological characterization of the three major proteins of vesicular stomatitis virus.
The three major proteins of vesicular stomatitis virus-Indiana, glycoprotein (G), nucleoprotein (N), and membrane protein (M), were isolated and characterized by means of specific monocomponent antisera. G, N, and M proteins are distinct, nonrelated antigens with specific serological properties. The G protein is the only antigen inducing the formation of virus-neutralizing antibodies and was shown to confer immunity to mice. Specific complement-fixing and precipitating activity was demonstrated for each of the three antisera. The future use of isolated rhabdovirus components and of monospecific antisera is considered for therapeutic and diagnostic purposes as well as for virus strain differentiation and classification work.
D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification*; D001666:Binding Sites, Antibody; D002462:Cell Membrane / Q000276:immunology; D003168:Complement Fixation Tests; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000302:isolation & purification; D007106:Immune Sera; D007525:Isoelectric Focusing; D008715:Methionine; D009500:Neutralization Tests; D009698:Nucleoproteins / Q000302:isolation & purification; D013462:Sulfur Radioisotopes; D014611:Vaccination; D014721:Vesicular stomatitis Indiana virus / Q000032:analysis / Q000276:immunology*; D014764:Viral Proteins / Q000302:isolation & purification*; D014765:Viral Vaccines
D000956:Antigens, Viral; D006023:Glycoproteins; D007106:Immune Sera; D009698:Nucleoproteins; D013462:Sulfur Radioisotopes; D014764:Viral Proteins; D014765:Viral Vaccines; D008715:Methionine