A filter assay for thymidylate synthetase using 5-fluoro-2'-deoxyuridylate as an active site titrant.
D000818:Animals; D001665:Binding Sites; D006528:Carcinoma, Hepatocellular; D002474:Cell-Free System; D003593:Cytoplasm; D003854:Deoxyribonucleotides; D004352:Drug Resistance, Microbial; D005069:Evaluation Studies as Topic; D005374:Filtration; D005461:Fluorine; D007700:Kinetics; D007780:Lacticaseibacillus casei / Q000201:enzymology; D008113:Liver Neoplasms; D008722:Methods; D008727:Methotrexate; D008780:Methyltransferases / Q000032:analysis*; D009374:Neoplasms, Experimental; D051381:Rats; D013053:Spectrophotometry; D013763:Tetrahydrofolates; D013940:Thymidylate Synthase / Q000032:analysis; D014316:Tritium; D014500:Uracil Nucleotides
D003854:Deoxyribonucleotides; D013763:Tetrahydrofolates; D014500:Uracil Nucleotides; D014316:Tritium; D005461:Fluorine; D008780:Methyltransferases; D013940:Thymidylate Synthase; D008727:Methotrexate