Structural characteristics and nucleotide sequence analysis of genomic RNA from RD-114 virus and feline RNA tumor viruses.
The results of molecular hybridization experiments have demonstrated that the RNA genome of RD-114 virus has extensive nucleotide sequence homology with the RNA genome of Crandell virus, an endogenous type C virus of cats, but only limited homology with the RNA genomes of feline sarcoma virus and feline leukemia virus. The genomic RNAs of RD-114 virus and Crandell virus also had identical sedimentation coefficients of 50S. A structural rearrangement of genomic RNA did not exist within released RD-114 virions, whereas a structural rearrangement of genomic RNA did occur within feline sarcoma virions and feline leukemia virions after release from virus-producing cells.
D000818:Animals; D001483:Base Sequence; D001859:Bone Neoplasms; D002415:Cats; D002460:Cell Line; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D002501:Centrifugation, Zonal; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D006801:Humans; D005256:Leukemia Virus, Feline / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D009858:Oncogenic Viruses / Q000032:analysis*; D012328:RNA Viruses / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D051381:Rats; D012190:Retroviridae / Q000032:analysis*; D012208:Rhabdomyosarcoma / Q000382:microbiology*; D013395:Sucrose; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D012367:RNA, Viral; D014316:Tritium; D013395:Sucrose; D004254:DNA Nucleotidyltransferases; D014529:Uridine