Golgi fractions prepared from rat liver homogenates. II. Biochemical characterization.
The three Golgi fractions isolated from rat liver homogenates by the procedure given in the companion paper account for 6-7% of the protein of the total microsomal fraction used as starting preparation. The lightest, most homogeneous Golgi fraction (GF(1)) lacks typical "microsomal" activities, e.g., glucose-6-phosphatase, NADPH-cytochrome c-reductase, and cytochrome P-450. The heaviest, most heterogeneous fraction (GF(3)) is contaminated by endoplasmic reticulum membranes to the extent of approximately 15% of its protein. The three fractions taken together account for nearly all the UDP-galactose: N-acetyl-glucosamine galactosyltransferase of the parent microsomal fraction, and for approximately 70% of the activity of the original homogenate. Omission of the ethanol treatment of the animals reduces the recovery by half. The transferase activity is associated with the membranes of the Golgi elements, not with their content. Galactose is transferred not only to N-acetyl-glucosamine but also to an unidentified lipid-soluble component.
D000818:Animals; D002458:Cell Fractionation; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003579:Cytochrome Reductases / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology; D005690:Galactose; D005944:Glucosamine; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D006056:Golgi Apparatus / Q000201:enzymology / Q000378:metabolism*; D006602:Hexosyltransferases / Q000378:metabolism; D008099:Liver / Q000166:cytology*; D008297:Male; D008566:Membranes / Q000201:enzymology; D008862:Microsomes, Liver / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D051381:Rats
D011506:Proteins; D000431:Ethanol; D003577:Cytochrome P-450 Enzyme System; D003579:Cytochrome Reductases; D006602:Hexosyltransferases; D005952:Glucose-6-Phosphatase; D005944:Glucosamine; D005690:Galactose