title: Hydroxyurea-induced accumulation of short fragments during polyoma DNA replication. I. Characterization of fragments.
abstract: Hydroxyurea treatment of 3T6 mouse fibroblast cells infected with polyoma virus resulted within 15 min in more than a 20-fold reduction of the rate of both viral and cellular DNA synthesis. After the initial rapid inhibition, the rate of DNA synthesis remained essentially constant for at least 2 h. In the inhibited cells viral DNA accumulated as short chains with a sedimentation coefficient of about 4S (hydroxyurea fragments). A variable proportion of these fragments was released from the template strands when the viral DNA was extracted by the Hirt procedure. Reannealing experiments demonstrated that hydroxyurea fragments were polyoma-specific and probably synthesized on both parental strands at the replication forks.
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient; D002848:Chromatography, DEAE-Cellulose; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication / Q000187:drug effects*; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis*; D004926:Escherichia coli / Q000201:enzymology; D005092:Exonucleases / Q000378:metabolism; D005347:Fibroblasts; D006918:Hydroxyurea / Q000494:pharmacology*; D051379:Mice; D011120:Polyomavirus / Q000032:analysis / Q000254:growth & development / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium
chemical_list: D004279:DNA, Viral; D014316:Tritium; D004247:DNA; D005092:Exonucleases; D013936:Thymidine; D006918:Hydroxyurea