Direct measurement of proton binding to the active ternary complex of pig heart lactate dehydrogenase.
With the use of a glass electrode it is shown that one proton is taken up from the solvent when o-nitrophenylpyruvate forms a ternary complex with lactate dehydrogenase and NADH at pH8 and before the complex has rearranged to give products.
D000818:Animals; D001665:Binding Sites; D004566:Electrodes; D005898:Glass; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase; D008722:Methods; D009206:Myocardium / Q000201:enzymology*; D009243:NAD; D009574:Nitro Compounds; D011522:Protons; D011773:Pyruvates; D013050:Spectrometry, Fluorescence; D013552:Swine
D009574:Nitro Compounds; D011522:Protons; D011773:Pyruvates; D009243:NAD; D007770:L-Lactate Dehydrogenase