Comparison of the turnover patterns of total and individual muscle proteins in normal mice and those with hereditary muscular dystrophy.
1. The incorporation of amino acids into hindleg muscle proteins of normal and dystrophic mice was measured (1/2)h to 16 days after administration of the radioactive pulse. 2. Dystrophic animals showed a faster initial rate of incorporation into total and soluble proteins in the first few hours after injection, but the extent of incorporation relative to the size of the amino acid pool was similar in both. There was little difference between the overall degradation rates although this started later in the dystrophic proteins. An initial fast phase of degradation reached a plateau after 3 days whereupon the residual label in the protein remained constant up to 16 days after injection. 3. Analyses of individual radioactive proteins fractionated by polyacrylamide-gel electrophoresis showed that the distribution of label was similar in all the soluble proteins from normal and dystrophic muscle. Time-course experiments revealed that in dystrophic mice the two major soluble proteins of the muscle, creatine kinase and adenylate kinase, initially incorporated 2-3 times more label relative to the initial size of the precursor pool. This label was then lost equally rapidly and the final plateau value was much less than that in normal mice. This initial peak of activity was not observed in normal mice. 4. A group of dehydrogenases showed similar initial turnover patterns in both dystrophic and normal mice but the final plateau value was much higher in the former. 5. The results provide support for the hypothesis that there is no obvious defect in the protein synthetic machinery of dystrophic muscle. However, certain proteins do show anomalous turnover patterns relative to those in normal animals. A single structural gene mutation giving rise to one particularly unstable and readily degradable muscle protein is excluded as the cause of the dystrophy.
D000249:Adenosine Monophosphate; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002250:Carbon Radioisotopes; D003402:Creatine Kinase / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005796:Genes; D051379:Mice; D009124:Muscle Proteins / Q000378:metabolism*; D009136:Muscular Dystrophies / Q000201:enzymology / Q000209:etiology / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010770:Phosphotransferases / Q000378:metabolism; D012519:Sarcoplasmic Reticulum / Q000378:metabolism; D013997:Time Factors
D000596:Amino Acids; D002250:Carbon Radioisotopes; D009124:Muscle Proteins; D000249:Adenosine Monophosphate; D010770:Phosphotransferases; D003402:Creatine Kinase