Pre- and post-junctional effects of 1-fluoro-2,4-dinitrobenzene at the frog neuromuscular junction.
1. FDNB increased by 60-90% the depolarization of the end-plate produced by applied carbachol in frog sciatic nerve-sartorius muscle preparations.2. In partially curarized preparations, FDNB (0.4 mM) increased the amplitude of the end-plate potential by a factor of 1.8.3. The quantal content of end-plate potentials was increased by FDNB (2 mM) as determined by the method of failures.4. After approximately 25-35 min, neuromuscular transmission was blocked by 0.4 mM-FDNB, as evidenced by abolition of neurally elicited end-plate potentials. At this stage miniature end-plate potentials could still be recorded, which indicates that the neuromuscular block was presynaptic.5. FDNB (0.4 mM) increased miniature end-plate potential frequency several hundred-fold when the Ringer solution contained normal calcium concentration (1.8 mM) or 0.45 mM calcium and 5.4 mM magnesium.6. During the first 60 min of exposure to 0.4 mM-FDNB there was a slight drop (4-6 mV) in resting potentials of muscle fibres. During this period directly initiated action potentials showed a marked decrease in the rate of repolarization and a small decrease in the amplitude and rate of rise.7. Using the technique of point voltage clamping in tetrodotoxin-treated muscles, it has been found that FDNB almost completely abolished the active increase in g(K) during stepwise depolarization of the nonjunctional muscle fibre membrane from -90 to 0 mV. The passive outward leakage current appeared unaffected by FDNB.
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001001:Anura; D002118:Calcium; D002217:Carbachol / Q000494:pharmacology; D004558:Electric Stimulation; D004594:Electrophysiology; D005459:Fluorides / Q000494:pharmacology*; D066298:In Vitro Techniques; D007478:Iontophoresis; D008274:Magnesium; D008564:Membrane Potentials / Q000187:drug effects; D009469:Neuromuscular Junction / Q000187:drug effects*; D009578:Nitrobenzenes / Q000494:pharmacology*; D012584:Sciatic Nerve; D009435:Synaptic Transmission / Q000187:drug effects; D013779:Tetrodotoxin / Q000494:pharmacology
D009578:Nitrobenzenes; D013779:Tetrodotoxin; D002217:Carbachol; D008274:Magnesium; D005459:Fluorides; D002118:Calcium