Anti-anabolic effects of adenosine 3':5'-cyclic monophosphate. Inhibition of protein synthesis.
1. Evidence is presented that cyclic AMP inhibits the incorporation of l-[4,5-(3)H]leucine into protein in a cell-free system from rat liver. This inhibition occurs after aminoacyl-tRNA formation. 2. Microsomal fractions, isolated after the incubation of postmitochondrial supernatant with cyclic AMP and ATP, show a diminished ability to synthesize protein. Both cyclic AMP and ATP are required for this effect. 3. A possible physiological role for the anti-anabolic action of cyclic AMP is discussed in terms of the control of gluconeogenesis.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002474:Cell-Free System; D002498:Centrifugation; D002940:Circadian Rhythm; D000242:Cyclic AMP / Q000494:pharmacology*; D005943:Gluconeogenesis / Q000187:drug effects; D066298:In Vitro Techniques; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D010728:Phosphoenolpyruvate; D014176:Protein Biosynthesis; D012343:RNA, Transfer / Q000096:biosynthesis; D051381:Rats; D046249:Transfer RNA Aminoacylation; D014316:Tritium; D014461:Ultracentrifugation
D014316:Tritium; D010728:Phosphoenolpyruvate; D000255:Adenosine Triphosphate; D012343:RNA, Transfer; D000242:Cyclic AMP; D007930:Leucine