Localization of replicating DNA of simian virus 40 in monkey kidney cells.
Approximately 80-85% of the DNA synthesized by cells infected with simian virus 40 during a short pulse with [(3)H]thymidine is associated with host nuclear membrane components, as defined by the M-band technique. A substantial portion of this membrane-associated DNA has been shown to be viral specific by sedimentation studies and by DNA.DNA hybridization. Examination of DNA associated and unassociated with nuclear M-band by neutral sucrose gradient analysis suggested that while replicating DNA of simian virus 40 is attached to the nuclear membrane, mature component I DNA of simian virus 40 is released from the membrane upon completion of replication.
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D004261:DNA Replication; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis*; D000882:Haplorhini; D007668:Kidney; D008566:Membranes / Q000032:analysis; D009693:Nucleic Acid Hybridization; D013539:Simian virus 40; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D004279:DNA, Viral; D014316:Tritium; D013936:Thymidine