The nucleotide sequence complexity of avian tumor virus RNA.
The nucleotide sequence complexity of 60-70S RNA of Rous sarcoma virus was determined from the molar yields of 11 pure oligonucleotides of known chain length, obtained from [(32)P]RNA of Rous sarcoma virus by T1 RNase digestion and two-dimensional polyacrylamide gel electrophoresis. We calculate an apparent chain length of about 9800 nucleotides, corresponding to a molecular weight of 3.4 x 10(6). Assuming the molecular weight of 60-70S RNA of Rous sarcoma virus to be about 10(7), our data suggest that the virus genome consists of identical, or very similar, nucleotide sequences repeated three to four times.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000032:analysis*; D001483:Base Sequence; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D005838:Genotype; D006868:Hydrolysis; D008970:Molecular Weight; D009841:Oligonucleotides / Q000032:analysis*; D010761:Phosphorus Radioisotopes; D012367:RNA, Viral / Q000032:analysis*; D012260:Ribonucleases
D009841:Oligonucleotides; D010761:Phosphorus Radioisotopes; D012367:RNA, Viral; D012260:Ribonucleases