Molecular weight determination of Sendai and Newcastle disease virus RNA.
The molecular weights of Sendai and Newcastle disease virus RNA were estimated by sedimentation in sucrose gradients and by length measurements in the electron microscope under both denaturing and nondenaturing conditions. Sedimentation analyses under denaturing conditions yielded molecular weight estimates of 2.3 x 10(6) to 2.6 x 10(6), whereas length measurements yielded estimates of 5.2 x 10(6) to 5.6 x 10(6) for both denatured and nondenatured viral RNA. It would appear that the conditions of denaturation used (99% dimethyl sulfoxide at 26 C, and reaction with 1.1 M formaldehyde for 10 min at 60 C) do not equally denature parainfluenza virus RNA and other RNAs, such as cellular rRNA, 45S rRNA precursor, and R17 RNA.
D000818:Animals; D002250:Carbon Radioisotopes; D002417:Cattle; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D004121:Dimethyl Sulfoxide; D004591:Electrophoresis, Polyacrylamide Gel; D005557:Formaldehyde; D006358:Hot Temperature; D007668:Kidney; D008854:Microscopy, Electron; D008970:Molecular Weight; D009522:Newcastle disease virus / Q000032:analysis*; D009691:Nucleic Acid Denaturation; D010222:Parainfluenza Virus 1, Human / Q000032:analysis* / Q000254:growth & development; D010761:Phosphorus Radioisotopes; D012367:RNA, Viral / Q000032:analysis*; D013395:Sucrose; D014316:Tritium
D002250:Carbon Radioisotopes; D010761:Phosphorus Radioisotopes; D012367:RNA, Viral; D014316:Tritium; D005557:Formaldehyde; D013395:Sucrose; D004121:Dimethyl Sulfoxide