A rapid method for collecting and assaying gradient fractions for radioactive macromolecules.
A rapid method is described for collecting gradients after centrifugation, and assaying them for acid-precipitable radioactivity. An entire gradient is collected and processed on a single glass-fibre filter disc. This method allows one easily to assay six gradients for acid-precipitable radioactivity in less than 2h.
D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D004279:DNA, Viral / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D005374:Filtration; D005898:Glass; D000882:Haplorhini; D007553:Isotope Labeling; D007668:Kidney; D008722:Methods; D013539:Simian virus 40; D013395:Sucrose; D013936:Thymidine; D013997:Time Factors; D014316:Tritium
D004279:DNA, Viral; D014316:Tritium; D013395:Sucrose; D013936:Thymidine