A DNA fragment of Herpes simplex 2 and its transcription in human cervical cancer tissue.
A human cervical tumor, free of detectable infectious herpes simplex 2 virus, contained a fragment comprising 39% of herpes viral DNA. Renaturation kinetics indicate that an average of 1 to 3.5 DNA fragments of herpes simplex virus are present per cell, depending on the ploidy of the cells in this particular tumor. Virus-specific sequences were found linked to highly repetitive sequences of host DNA, which reassociated under conditions designed to preclude reassociation of viral sequences. The tumor also contained RNA transcripts complementary to 5% of the viral DNA. The fraction of viral DNA template transcribed in the cervical tumor is considerably less than that transcribed in productively infected cells (50%).
D004273:DNA, Neoplasm / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D005260:Female; D006801:Humans; D007700:Kinetics; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D009695:Nucleic Acid Renaturation; D012334:RNA, Neoplasm / Q000378:metabolism; D012367:RNA, Viral / Q000032:analysis; D018139:Simplexvirus / Q000302:isolation & purification; D013698:Templates, Genetic; D014158:Transcription, Genetic; D002583:Uterine Cervical Neoplasms / Q000378:metabolism / Q000382:microbiology
D004273:DNA, Neoplasm; D004279:DNA, Viral; D012334:RNA, Neoplasm; D012367:RNA, Viral