Simian virus 40-host cell interactions. I. Temperature-sensitive regulation of SV40 T antigen in 3T3 mouse cells transformed by the ts*101 temperature-sensitive early mutant of SV40.
BALB/3T3 and Swiss/3T3 mouse cells transformed at permissive temperature (33 C) by the early temperature-sensitive mutant of simian virus 40 (SV40), ts(*)101, exhibited a temperature-dependent modulation of SV40 tumor (T) antigen as assayed by immunofluorescence. The percentage of T antigen-positive nuclei in ts(*)101 transformed cells was reduced at restrictive temperature (39 C) when compared to 33 C and to wild-type SV40 transformed cells at either 33 C or 39 C. The percentage of T antigen-positive nuclei in ts(*)101 transformed cells returned to the 33 C control level when the cells were shifted from 39 to 33 C. The ts(*)101 transformed cells could be superinfected with wild-type, but not ts(*)101, virions at 39 C as assayed by an increase in T antigen-positive nuclei.
D000327:Adsorption; D000818:Animals; D000956:Antigens, Viral; D002478:Cells, Cultured; D004279:DNA, Viral / Q000096:biosynthesis; D005455:Fluorescent Antibody Technique; D051379:Mice; D008815:Mice, Inbred Strains; D009154:Mutation; D013539:Simian virus 40 / Q000276:immunology* / Q000378:metabolism; D013696:Temperature; D014170:Transformation, Genetic; D014764:Viral Proteins
D000956:Antigens, Viral; D004279:DNA, Viral; D014764:Viral Proteins