The in vitro synthesis of avian myeloblastosis viral RNA sequences.
Isolated nuclei, prepared from myeloblasts of chicks infected with avian myeloblastosis virus, synthesize RNA sequences present in avian myeloblastosis viral RNA. These sequences are also formed during transcription of chromatin, isolated from myeloblasts, by DNA-dependent RNA polymerases purified from Escherichia coli or calfthymus. In the latter case, transcription is alpha-amanitin sensitive. Formation of hybrids between RNA and avian myeloblastosis virus DNA probes has been monitored by the combined use of ribonucleases A, T(1), and H, and ribonucleases specific for single strands.
D000818:Animals; D001354:Avian Leukosis Virus / Q000378:metabolism*; D009189:Avian Myeloblastosis Virus / Q000378:metabolism*; D001854:Bone Marrow Cells; D002467:Cell Nucleus / Q000378:metabolism; D002471:Cell Transformation, Neoplastic; D002642:Chick Embryo; D002843:Chromatin / Q000302:isolation & purification / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D066298:In Vitro Techniques; D009693:Nucleic Acid Hybridization; D010761:Phosphorus Radioisotopes; D012367:RNA, Viral / Q000096:biosynthesis*; D012260:Ribonucleases / Q000378:metabolism; D013698:Templates, Genetic; D013950:Thymus Gland / Q000201:enzymology; D014158:Transcription, Genetic; D014316:Tritium; D014779:Virus Replication
D002843:Chromatin; D004277:DNA, Single-Stranded; D010761:Phosphorus Radioisotopes; D012367:RNA, Viral; D014316:Tritium; D012321:DNA-Directed RNA Polymerases; D012260:Ribonucleases