Virus susceptibility of a new simian cell line of fetal origin.
The cultivation and characterization of a cell line derived from the foreskin of a fetal, rhesus monkey (rhfs2) are described. This cell line has proven satisfactory for isolation and assay of a variety of viral agents of human and simian origin. Virus titrations performed on foreskin cells yielded titers comparable to, or higher than, those obtained in rhesus monkey kidney cells (LLC-MK2). Replicate isolation attempts in our laboratory from simian clinical specimens have proven rhfs2 superior to LLC-MK2 for ease of detection and frequency of isolation.
D000256:Adenoviridae / Q000302:isolation & purification; D000257:Adenoviridae Infections / Q000662:veterinary; D001003:Anal Canal / Q000382:microbiology; D000818:Animals; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D005069:Evaluation Studies as Topic; D000882:Haplorhini; D007668:Kidney; D008251:Macaca; D008297:Male; D008992:Monkey Diseases / Q000382:microbiology; D009055:Mouth / Q000382:microbiology; D010413:Penis; D012141:Respiratory Tract Infections / Q000662:veterinary; D014776:Virus Cultivation; D014780:Viruses / Q000254:growth & development* / Q000302:isolation & purification