Human serum albumin phenotype activation in mouse hepatoma--human leukocyte cell hybrids.
Murine hepatoma cells that secreted mouse serum albumin were fused with human leukocytes that did not produce albumin. The resulting hybrids secreted both mouse and human serum albumin, as demonstrated by immuno-electrophoretic techniques. The activation of the human genome suggests that mapping genes governing specialized functions in somatic cell hybrids may be accomplished by using adifferentiated human cells as a parental line.
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000201:enzymology; D002460:Cell Line; D002874:Chromosome Mapping; D004794:Enzyme Repression; D005796:Genes; D008040:Genetic Linkage; D005838:Genotype; D006147:Guanine; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology / Q000378:metabolism*; D007042:Hypoxanthines; D007122:Immunoelectrophoresis; D007962:Leukocytes / Q000201:enzymology; D008113:Liver Neoplasms; D051379:Mice; D010430:Pentosyltransferases / Q000302:isolation & purification; D010641:Phenotype; D012709:Serum Albumin / Q000096:biosynthesis* / Q000378:metabolism
D007042:Hypoxanthines; D012709:Serum Albumin; D006147:Guanine; D010430:Pentosyltransferases