Purification of Clostridium perfringens phospholipase C (alpha-toxin) by affinity chromatography on agarose-linked egg-yolk lipoprotein.
D000818:Animals; D001681:Biological Assay; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003016:Clostridium perfringens / Q000201:enzymology*; D004355:Drug Stability; D004530:Egg Yolk; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006461:Hemolysis; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D005779:Immunodiffusion; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000302:isolation & purification; D008074:Lipoproteins; D051379:Mice; D008970:Molecular Weight; D010740:Phospholipases / Q000302:isolation & purification* / Q000506:poisoning; D012756:Sheep; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013997:Time Factors; D014118:Toxins, Biological / Q000302:isolation & purification* / Q000506:poisoning; D014461:Ultracentrifugation
D007527:Isoenzymes; D008074:Lipoproteins; D014118:Toxins, Biological; D010740:Phospholipases