Quantitative assessment of hemadsorption by myxoviruses: virus hemadsorption assay.
The standardization and quantitative evaluation of an assay for myxoviruses, based on the enumeration of individual infected clone 1-5C-4 cells manifesting hemadsorption within 24 h of infection, are described. Hemadsorption was detectable earlier than immunofluorescence in infected cells or hemagglutinins in culture medium. The relationship between virus concentration and cells exhibiting hemadsorption was linear. The assay was highly precise, sensitive, and reproducible.
D000818:Animals; D002460:Cell Line; D002999:Clone Cells; D003228:Conjunctiva; D006224:Cricetinae; D004912:Erythrocytes / Q000276:immunology; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs / Q000276:immunology; D000882:Haplorhini; D006367:HeLa Cells; D006382:Hemadsorption; D006386:Hemagglutination Tests; D006801:Humans; D007668:Kidney; D007739:L Cells; D008722:Methods; D051379:Mice; D009975:Orthomyxoviridae / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification; D018108:Respirovirus / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification; D012698:Serologic Tests / Q000592:standards