Hepatic drug hydroxylation and lipid peroxidation in riboflavin-deficient rats.
The effect of riboflavin deficiency and phenobarbital pretreatment on drug hydroxylation and lipid peroxidation was investigated. A significant decrease in aniline and acetanilide hydroxylation as well as NADPH-linked and ascorbate-induced lipid peroxidation was observed during 4- and 7-week riboflavin deficiency in both adult male and adult female rats. The drug-hydroxylation and lipid-peroxidation activities were further lowered with the increase in riboflavin deficiency. The phenobarbital pretreatment induced aniline and acetanilide hydroxylase activity even in riboflavin-deficient animals. Drug hydroxylation inhibits lipid peroxidation in both deficient and normal rats. The administration of riboflavin was followed by a significant increase in drug hydroxylation and lipid peroxidation.
D000083:Acetanilides / Q000378:metabolism; D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D005260:Female; D006900:Hydroxylation; D007700:Kinetics; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D009243:NAD / Q000378:metabolism; D009929:Organ Size; D010101:Oxygen Consumption; D010544:Peroxidases / Q000378:metabolism; D010545:Peroxides / Q000378:metabolism*; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D010634:Phenobarbital; D011506:Proteins / Q000032:analysis; D051381:Rats; D012256:Riboflavin; D012257:Riboflavin Deficiency / Q000378:metabolism*; D012737:Sex Factors
D000083:Acetanilides; D000814:Aniline Compounds; D010545:Peroxides; D004364:Pharmaceutical Preparations; D011506:Proteins; D009243:NAD; D010544:Peroxidases; D001205:Ascorbic Acid; D012256:Riboflavin; D010634:Phenobarbital