title: Threonine locus of Escherichia coli K-12: genetic structure and evidence for an operon.
abstract: Three genes, thrA, thrB, and thrC, were previously defined and localized in the threonine locus of Escherichia coli K-12. thrA, thrB, and thrC specify the enzymes aspartokinase I-homoserine dehydrogenase I, homoserine kinase, and threonine synthetase, respectively. A complementation analysis of the threonine cluster using derivatives of a lambda phage carrying the threonine genes (lambdadthr(c)) demonstrates that: (i) thrB and thrC each consist of a single cistron; and (ii) thrA is composed of two cistrons, thrA(1) and thrA(2), although it specifies a single polypeptide chain. thrA(1) and thrA(2) correspond to aspartokinase I and homoserine dehydrogenase I, respectively. Their relative order is established. The demonstration of polar effects of mutations (nonsense or induced by phage Mu) in thrA and thrB is taken as evidence for the existence of a thrA thrB thrC operon, transcribed in this order.
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism; D001224:Aspartic Acid; D002474:Cell-Free System; D002874:Chromosome Mapping; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D005796:Genes; D005816:Genetic Complementation Test; D006714:Homoserine / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008190:Lyases / Q000378:metabolism; D009154:Mutation; D009876:Operon; D010770:Phosphotransferases / Q000378:metabolism; D010867:Pimelic Acids / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism; D013912:Threonine / Q000096:biosynthesis* / Q000378:metabolism; D014161:Transduction, Genetic
chemical_list: D010867:Pimelic Acids; D013912:Threonine; D001224:Aspartic Acid; D006714:Homoserine; D000429:Alcohol Oxidoreductases; D007770:L-Lactate Dehydrogenase; D010770:Phosphotransferases; D011770:Pyruvate Kinase; D008190:Lyases