Lack of temperature-sensitivity of rat liver pyruvate kinase.
Rat liver pyruvate kinase, prepared by Sephadex treatment of a 10(5)g supernatant in phosphate buffer, is quite stable and gives reproducible results when a variety of parameters are altered in the enzyme assay. Incubation of this preparation at 25 degrees C or 0-2 degrees C has no effect on the activation by fructose 1,6-diphosphate or inhibition by ATP or alanine.
D000255:Adenosine Triphosphate; D000409:Alanine; D000818:Animals; D002850:Chromatography, Gel; D004355:Drug Stability; D004789:Enzyme Activation; D006597:Fructose-Bisphosphatase; D006598:Hexosediphosphates; D008099:Liver / Q000201:enzymology*; D008297:Male; D011770:Pyruvate Kinase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013997:Time Factors
D006598:Hexosediphosphates; D000255:Adenosine Triphosphate; D011770:Pyruvate Kinase; D006597:Fructose-Bisphosphatase; D000409:Alanine