Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. VI. Characterization of the DNA from five nondefective hybrid viruses.
Certain biophysical characteristics of the DNA from each of the five nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid viruses (Ad2(+)ND(1), Ad2(+)ND(2), Ad2(+)ND(3), Ad2(+)ND(4), Ad2(+)ND(5)) have been determined. The guanine plus cytosine content varied from 55 to 57% and was not significantly different from that of nonhybrid Ad2 (56%), and the hybrid DNA molecules had mean molecular lengths which were similar to that of the standard, Ad2. The Ad2 and SV40 components of each hybrid were linked by alkali-resistant, presumably covalent bonds. The percentage of SV40 DNA in each hybrid virus was determined by hybridization with SV40 complementary RNA in a calibrated system. The results indicate that each hybrid virus DNA contains a different percentage of SV40 nucleotide sequences. The estimated size of the SV40 DNA component varies from 48,000 daltons for Ad2(+)ND(3) to 840,000 daltons for Ad2(+)ND(4), the latter being equivalent to between one-fourth and one-third of the SV40 genome.
D000256:Adenoviridae / Q000032:analysis*; D001483:Base Sequence; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D003596:Cytosine / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D006147:Guanine / Q000032:analysis; D006358:Hot Temperature; D006824:Hybridization, Genetic; D008854:Microscopy, Electron; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral; D013539:Simian virus 40 / Q000032:analysis*; D014316:Tritium; D014776:Virus Cultivation
D002247:Carbon Isotopes; D004279:DNA, Viral; D012367:RNA, Viral; D014316:Tritium; D006147:Guanine; D003596:Cytosine