Isolation of an infectious ribonucleoprotein from vesicular stomatitis virus containing an active RNA transcriptase.
A ribonucleoprotein complex (TNP) containing an active RNA polymerase was isolated from purified vesicular stomatitis virus particles. The TNP sedimented through a sucrose gradient as a single band and appeared under the electron microscope as discrete long filaments in a spiral configuration. TNP contained one major and two minor polypeptides, but not the polypeptides associated with the outer coat of vesicular stomatitis virus. BHK-21 clone 13 cells could be infected with TNP, yielding infectious virus particles.
D000818:Animals; D001345:Autoradiography; D002460:Cell Line; D002918:Chymotrypsin; D002999:Clone Cells; D006224:Cricetinae; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006020:Glycopeptides / Q000032:analysis; D007668:Kidney; D008854:Microscopy, Electron; D009698:Nucleoproteins / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D010455:Peptides / Q000032:analysis; D012367:RNA, Viral / Q000096:biosynthesis; D012260:Ribonucleases; D013460:Sulfur Isotopes; D014316:Tritium; D014721:Vesicular stomatitis Indiana virus / Q000032:analysis* / Q000201:enzymology / Q000302:isolation & purification; D014764:Viral Proteins / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology
D006020:Glycopeptides; D009698:Nucleoproteins; D010455:Peptides; D012367:RNA, Viral; D013460:Sulfur Isotopes; D014764:Viral Proteins; D014316:Tritium; D012321:DNA-Directed RNA Polymerases; D012260:Ribonucleases; D002918:Chymotrypsin