Affinity column purification of protocollagen proline hydroxylase from chick embryos and further characterization of the enzyme.
D000478:Alkylation; D000596:Amino Acids / Q000032:analysis; D000645:Ammonium Sulfate; D000818:Animals; D001200:Ascaris; D002642:Chick Embryo; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003094:Collagen; D004229:Dithiothreitol; D004589:Electrophoresis, Disc; D004591:Electrophoresis, Polyacrylamide Gel; D006146:Guanidines; D008239:Lysine; D046911:Macromolecular Substances; D008623:Mercaptoethanol; D006899:Mixed Function Oxygenases / Q000032:analysis / Q000302:isolation & purification*; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010455:Peptides; D011134:Polysaccharides; D011393:Procollagen-Proline Dioxygenase / Q000302:isolation & purification; D012967:Sodium Dodecyl Sulfate; D014461:Ultracentrifugation
D000596:Amino Acids; D006146:Guanidines; D046911:Macromolecular Substances; D010455:Peptides; D011134:Polysaccharides; D012967:Sodium Dodecyl Sulfate; D008623:Mercaptoethanol; D003094:Collagen; D006899:Mixed Function Oxygenases; D011393:Procollagen-Proline Dioxygenase; D008239:Lysine; D000645:Ammonium Sulfate; D004229:Dithiothreitol