title: Close genetic linkage of the determinants of the ribitol and D-arabitol catabolic pathways in Klebsiella aerogenes.
abstract: Klebsiella aerogenes strain W70 has separate inducible pathways for the degradation of the pentitols ribitol and d-arabitol. These pathways are closely linked genetically as determined by transduction with phage PW52. There are two regulatory sites for the ribitol catabolic pathway as defined by loci for mutations to constitutive synthesis of ribitol dehydrogenase and d-ribulokinase, rbtB and rbtC. The two control sites are separated by a site represented by the dalB22 mutation. This mutation deprives the cell of the ability to induce synthesis of d-arabitol dehydrogenase and d-xylulokinase activities. Two additional regulatory mutations for the d-arabitol pathway, dalC31 and dalC37, map to the opposite side of rbtB13 relative to dalB22. The order of the genetic sites thus far determined for this region is dalK-dalD-dalC31, dalC37-rbtB13-dalB22-rbtC14-rbtD-rbtK, where dalK and dalD represent structural genes for the kinase and dehydrogenase of the d-arabitol pathway, respectively, and rbtK and rbtD represent the corresponding genes for the ribitol pathway. The two mutations that lead to constitutive synthesis of the d-arabitol-induced enzymes, dalC31 and dalC37, have different phenotypes with regard to their response to xylitol. The growth of dalC31 is inhibited by xylitol, but the toxicity can be reduced by increasing the levels of ribitol dehydrogenase either by induction with ribitol or by selection of a ribitol dehydrogenase-constitutive mutation.
mesh_terms: D000429:Alcohol Oxidoreductases / Q000096:biosynthesis; D001089:Arabinose; D002238:Carbohydrate Epimerases / Q000096:biosynthesis; D002474:Cell-Free System; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004790:Enzyme Induction; D005796:Genes; D008040:Genetic Linkage; D007709:Klebsiella / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D009154:Mutation; D010770:Phosphotransferases / Q000096:biosynthesis; D011995:Recombination, Genetic; D012266:Ribose; D013237:Stereoisomerism; D013402:Sugar Alcohols / Q000378:metabolism*; D014161:Transduction, Genetic; D014993:Xylitol / Q000378:metabolism; D014994:Xylose
chemical_list: D013402:Sugar Alcohols; D012266:Ribose; D014994:Xylose; D001089:Arabinose; D000429:Alcohol Oxidoreductases; D010770:Phosphotransferases; D002238:Carbohydrate Epimerases; D014993:Xylitol