Membrane proteins of uninfected and Rous sarcoma virus- transformed avian cells.
A method for preparing large membrane fragments and cell ghosts was developed for uninfected and Rous sarcoma virus-transformed chicken embryo fibroblasts in culture. Membrane proteins were analyzed by electrophoresis in acrylamide gels containing sodium dodecyl sulfate. A major amino-acid-containing component of uninfected cell membranes was greatly diminished in amount or absent in membranes of virus-transformed cells. This component, called MP-1, had an electrophoretic mobility in sodium dodecyl sulfate-containing gels similar to that of a protein of a mol wt of 1.42 x 10(5). MP-1 was not altered by changes in cell growth rate or in cells infected with the nontransforming virus RAV-1.
D000596:Amino Acids; D000818:Animals; D001358:Avian Sarcoma Viruses / Q000254:growth & development*; D002250:Carbon Radioisotopes; D002458:Cell Fractionation; D002462:Cell Membrane / Q000032:analysis*; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts; D005643:Fucose; D006023:Glycoproteins / Q000032:analysis; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D012526:Satellite Viruses / Q000254:growth & development; D012967:Sodium Dodecyl Sulfate; D013053:Spectrophotometry; D013936:Thymidine; D014316:Tritium
D000596:Amino Acids; D002250:Carbon Radioisotopes; D006023:Glycoproteins; D009363:Neoplasm Proteins; D011506:Proteins; D014316:Tritium; D005643:Fucose; D012967:Sodium Dodecyl Sulfate; D013936:Thymidine