Aspects of the encapsidation of simian virus 40 deoxyribonucleic acid.
Growing subcloned CV1-cells were infected with simian virus 40, and the time course of virus formation was determined. When infected cells were fractionated into cytoplasmic and nuclear fractions, most of the progeny virus particles were recovered in the cytoplasmic extract and not in the nuclei. This result was independent of the technique used for the preparation of nuclei and of the time after infection at which the extracts were prepared. Leakage of the virions from the nucleus occurred during the course of cell fractionation, suggesting that the nuclear membrane of the infected cells is damaged. Virions were found to accumulate in a nonlinear fashion, at the time when the number of viral deoxyribonucleic acid (DNA) molecules increases linearly with time after infection. This suggests that the size of the intracellular pool of capsid proteins increases constantly during the late phase of virus replication. Progeny viral DNA to become encapsidated is withdrawn at random from the pool of replicated DNA molecules.
D000818:Animals; D002458:Cell Fractionation; D002460:Cell Line; D002467:Cell Nucleus / Q000382:microbiology; D002499:Centrifugation, Density Gradient; D002999:Clone Cells; D003593:Cytoplasm / Q000382:microbiology; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis; D000882:Haplorhini; D007668:Kidney; D008854:Microscopy, Electron; D013539:Simian virus 40 / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
D004279:DNA, Viral; D014764:Viral Proteins; D014316:Tritium; D013936:Thymidine