Cell-free translation of messenger RNA of simian virus 40: synthesis of the major capsid protein.
Extracts of wheat germ are capable of synthesizing the major capsid protein of simian virus 40. Poly(A)-containing RNA from BS-C-1 cells infected with simian virus 40 directed the synthesis of a novel polypeptide that migrates in polyacrylamide gels together with the major capsid polypeptide of simian virus 40, VP-1. The patterns of the major tryptic peptides of purified VP-1 and the novel polypeptide synthesized in vitro were identical after two-dimensional paper electrophoresis. The novel polypeptide was not synthesized in response to poly(A)-rich RNA from uninfected cells or from virus-infected cells treated with cytosine arabinoside. Messenger RNA from infected cells purified by selective hybridization to DNA of simian virus 40 directs the synthesis of a major polypeptide of electrophoretic mobility similar to that of VP-1 of simian virus 40. This approach should prove useful in identifying additional products specified by DNA tumor viruses.
D000227:Adenine Nucleotides / Q000032:analysis; D000818:Animals; D001345:Autoradiography; D002460:Cell Line; D002474:Cell-Free System; D004279:DNA, Viral / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D000882:Haplorhini; D007668:Kidney; D008715:Methionine / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010455:Peptides / Q000302:isolation & purification; D010944:Plants; D011119:Polynucleotides / Q000032:analysis; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D012367:RNA, Viral / Q000302:isolation & purification / Q000378:metabolism*; D013539:Simian virus 40 / Q000378:metabolism*; D013462:Sulfur Radioisotopes; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis*
D000227:Adenine Nucleotides; D004279:DNA, Viral; D010455:Peptides; D011119:Polynucleotides; D012333:RNA, Messenger; D012367:RNA, Viral; D013462:Sulfur Radioisotopes; D014764:Viral Proteins; D008715:Methionine