Electron transport-linked compared with proton-induced ATP generation in Thiobacillus novellus.
The apparently soluble electron-transport system, that does not sediment when centrifuged at 144,000 x g or 300,000 x g for 3 hr, catalyzes oxidative phosphorylation with an efficiency comparable to that of an intact mitochondrial system. While the proton-induced phosphorylation occurs in whole cells, crude cell-free extracts, and supernatants from low-speed centrifugation, it does not occur in either the 144,000 or 300,000 x g supernatant fractions. The data show that oxidative energy can be conserved as ATP under conditions that are incompatible with the basic postulate of the chemiosmotic hypothesis.
D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D001205:Ascorbic Acid / Q000378:metabolism; D002384:Catalysis; D002458:Cell Fractionation; D002474:Cell-Free System; D004579:Electron Transport; D005971:Glutamates / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009243:NAD / Q000378:metabolism; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D013347:Subcellular Fractions / Q000378:metabolism; D013386:Succinates / Q000378:metabolism; D013855:Thiobacillus / Q000166:cytology / Q000378:metabolism*; D013885:Thiosulfates / Q000378:metabolism; D014461:Ultracentrifugation; D014475:Uncoupling Agents
D005971:Glutamates; D013386:Succinates; D013885:Thiosulfates; D014475:Uncoupling Agents; D009243:NAD; D000255:Adenosine Triphosphate; D001205:Ascorbic Acid