Selective media in the isolation of tubercle bacilli from tissues.
Direct culture on Lowenstein-Jensen slopes and on three media made selective for tubercle bacilli by the addition of four antibacterial agents was compared with guinea-pig inoculation on 490 tissue specimens. Tubercle bacilli were obtained from 15 specimens by culture and 14 by guinea-pig inoculation; only one specimen was positive by guinea-pig and not by culture. The most efficient culture medium was a selective 7H11 slope. Routine guinea-pig inoculation has been replaced by a wider range of culture procedures.
D000666:Amphotericin B; D000818:Animals; D002228:Carbenicillin; D003470:Culture Media; D004717:Endometrium / Q000382:microbiology; D005260:Female; D006168:Guinea Pigs; D008099:Liver / Q000382:microbiology; D008198:Lymph Nodes / Q000382:microbiology; D008722:Methods; D009163:Mycobacterium bovis / Q000302:isolation & purification*; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D011113:Polymyxins; D014295:Trimethoprim
D003470:Culture Media; D011113:Polymyxins; D000666:Amphotericin B; D014295:Trimethoprim; D002228:Carbenicillin