A spectrophotometric assay of beta-lactamase action on penicillins.
A new method for measuring the enzymic hydrolysis of the beta-lactam ring in penicillins is described. The change in extinction in the u.v. region is determined. The method is sensitive (50mum-benzylpenicillin can be used) and convenient.
D000588:Amines; D000667:Ampicillin; D002228:Carbenicillin; D006868:Hydrolysis; D010397:Penicillanic Acid; D010400:Penicillin G; D010405:Penicillinase; D010406:Penicillins; D013056:Spectrophotometry, Ultraviolet
D000588:Amines; D010406:Penicillins; D000667:Ampicillin; D010397:Penicillanic Acid; D010405:Penicillinase; D002228:Carbenicillin; D010400:Penicillin G