title: Association of DNA-dependent and  -independent ribonucleoside triphosphatase activities with dnaB gene product of Escherichia coli.
abstract: Preparations of E. coli dnaB gene product contain ribonucleoside triphosphatase activity that is stimulated 10-fold by DNA. The products of the triphosphatase activity are nucleoside diphosphates and P(i). The dnaB complementing activity in the varphiX174 DNA-dependent system and these triphosphatase activities copurify over the last 20-fold of an extensive (about 40,000-fold) purification procedure. Acrylamide gel electrophoresis of the purified material shows a single band of protein coincident with eluted dnaB complementing and DNA-dependent and -independent nucleoside triphosphatase activities.
mesh_terms: D000251:Adenosine Triphosphatases / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange; D004279:DNA, Viral; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D005796:Genes; D006868:Hydrolysis; D010744:Phosphoric Monoester Hydrolases / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D011119:Polynucleotides; D014176:Protein Biosynthesis; D012265:Ribonucleotides; D013268:Stimulation, Chemical
chemical_list: D001426:Bacterial Proteins; D004279:DNA, Viral; D011119:Polynucleotides; D012265:Ribonucleotides; D000255:Adenosine Triphosphate; D010744:Phosphoric Monoester Hydrolases; D000251:Adenosine Triphosphatases