The homopolyadenylate and adjacent nucleotides at the 3'-terminus of 30-40s RNA subunits in the genome of murine sarcoma-leukemia virus.
Adenosine is the major 3'OH-terminal nucleoside of the 60-70S RNA genome of the murine sarcoma-leukemia virus, its 30-40S RNA subunits, and the poly(A) segments derived by RNase treatment of both RNA species, as determined by periodate oxidation-[(3)H]-borohydride reduction. The binding 30-40S RNA to oligo(dT)-cellulose suggests that most viral RNA subunits contain poly(A). The molecular weight of poly(A) derived from viral RNA by digestion with RNase and purified by affinity chromatography is 64,000-68,000, as determined by gel electrophoresis. From the size of poly(A) and the poly(A) content of viral RNA (1.6%), it is estimated that there is about one poly(A) segment for each viral 30-40S RNA subunit. The results of 3'-termini labeling with [(3)H]borohydride, in vivo labeling with [(3)H]adenosine, and base composition of [(32)P]poly(A) indicate that a homopoly(A) segment is located at the 3'-end of a 30-40S RNA subunit. The homogeneous poly(A) segments isolated from RNase T1 digests of 60-70S [(32)P]RNA consist of one cytidylate, one uridylate, and about 190 adenylate residues, while those isolated from RNase A digests consist exclusively of adenylate residues. These results indicate that -G(C,U)A(190)A(OH) is the 3'-terminal nucleotide sequence of the viral 30-40S RNA subunits.
D000227:Adenine Nucleotides / Q000032:analysis*; D000241:Adenosine / Q000032:analysis; D000818:Animals; D001483:Base Sequence; D001894:Borohydrides; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D017988:Gammaretrovirus / Q000032:analysis*; D005838:Genotype; D051379:Mice; D008970:Molecular Weight; D009711:Nucleotides / Q000032:analysis*; D010761:Phosphorus Radioisotopes; D011119:Polynucleotides / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D012260:Ribonucleases; D014316:Tritium
D000227:Adenine Nucleotides; D001894:Borohydrides; D009711:Nucleotides; D010761:Phosphorus Radioisotopes; D011119:Polynucleotides; D012367:RNA, Viral; D014316:Tritium; D012260:Ribonucleases; D000241:Adenosine