Transient inhibition of polyoma virus synthesis by sendai virus (parainfluenza I). II. Mechanism of the interference by inactivated virus.
The mechanism of the transient inhibition of polyoma virus synthesis by betapropiolactone-inactivated Sendai virus was studied. Polyoma virus early functions did not appear to be affected, although deoxyribonucleic acid (DNA) and structural protein synthesis were inhibited 60 and 35% respectively. The inhibition of macromolecular synthesis was not sufficient to account for the 90% inhibition of infectious progeny formation. Encapsidation of polyoma DNA into mature virions appears to be completely inhibited after superinfection by beta-propiolactone-inactivated Sendai virus. Ultraviolet irradiation of live or beta-propiolactone-inactivated Sendai virus preparations abolishes the interfering capacity, indicating that a functional Sendai virus ribonucleic acid molecule is the interfering component.
D000818:Animals; D002471:Cell Transformation, Neoplastic; D002499:Centrifugation, Density Gradient; D046508:Culture Techniques; D004279:DNA, Viral / Q000096:biosynthesis; D003609:Dactinomycin / Q000494:pharmacology; D005467:Floxuridine / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006386:Hemagglutination Tests; D006571:Heterocyclic Compounds; D007783:Lactones / Q000494:pharmacology; D051379:Mice; D010222:Parainfluenza Virus 1, Human / Q000187:drug effects; D011120:Polyomavirus / Q000254:growth & development* / Q000378:metabolism; D011422:Propionates; D012367:RNA, Viral / Q000096:biosynthesis; D011830:Radiation Effects; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014466:Ultraviolet Rays; D014762:Viral Interference; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000096:biosynthesis
D004279:DNA, Viral; D006571:Heterocyclic Compounds; D007783:Lactones; D011422:Propionates; D012367:RNA, Viral; D014764:Viral Proteins; D005467:Floxuridine; D014316:Tritium; D003609:Dactinomycin; D013936:Thymidine