In vitro polyoma DNA synthesis: studies on an early temperature-sensitive mutant.
The polyoma ts-a function was investigated by using an in vitro DNA-synthesizing system. A comparison of systems derived from ts25 (a ts-a group mutant)-and ts1260 (a late group mutant)-infected cells showed that the activation energies for DNA chain elongation and the mechanisms of discontinous growth were identical for both mutants.
D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D003597:Cytosine Nucleotides / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D051379:Mice; D009154:Mutation; D010761:Phosphorus Radioisotopes; D011120:Polyomavirus / Q000254:growth & development / Q000378:metabolism*; D013696:Temperature; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium
D003597:Cytosine Nucleotides; D004279:DNA, Viral; D010761:Phosphorus Radioisotopes; D014316:Tritium; D004247:DNA; D013936:Thymidine