Simian virus 40 T antigen binds to DNA.
The T antigen of simian virus 40, which may play a role in the control of viral DNA replication, is recovered from nuclei of cells transformed by simian virus 40 in several forms sedimenting at different rates. The large molecular weight forms are converted to the smallest (5 S) form by high salt, suggesting that they differ in the degree of aggregation. All the forms of the antigen bind efficiently to double-stranded DNA-cellulose columns at pH 6.2 and low salt, and elute in two fractions: one at pH 8.0 and low salt, the other at pH 8.0 and high salt. The antigen has little affinity for single-stranded DNA.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002482:Cellulose; D002499:Centrifugation, Density Gradient; D002845:Chromatography; D003168:Complement Fixation Tests; D006224:Cricetinae / Q000276:immunology; D004247:DNA; D007106:Immune Sera; D008970:Molecular Weight; D013539:Simian virus 40 / Q000276:immunology*
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D007106:Immune Sera; D002482:Cellulose; D004247:DNA