The preparation and analysis of a new trinucleotide from yeast ribonucleic acid, containing two adjacent 2'-O-methylpentose residues.
Quantities of the order of 10mg of three alkali-stable trinucleotides were prepared from yeast ribonucleic acid. Analyses showed that they were of the type N1m-N2m-N3p, where m signifies 2'-O-methylation of the pentose residue. One, Am-Um-Gp, is newly described.
D000469:Alkaline Phosphatase; D001483:Base Sequence; D002854:Chromatography, Paper; D004590:Electrophoresis, Paper; D004926:Escherichia coli; D008745:Methylation; D009841:Oligonucleotides / Q000032:analysis; D010429:Pentoses / Q000032:analysis; D010727:Phosphoric Diester Hydrolases; D011119:Polynucleotides / Q000032:analysis*; D012313:RNA / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D009841:Oligonucleotides; D010429:Pentoses; D011119:Polynucleotides; D012313:RNA; D000469:Alkaline Phosphatase; D010727:Phosphoric Diester Hydrolases