Extraction of equine infectious anemia immunodiffusion antigen with the aid of the chaotropic agent, thiocyanate.
Immunodiffusion antigen from spleens of horses infected with equine infectious anemia virus was prepared by methods employing freeze-thaw cycles and thiocyanate treatment. Thiocyanate (0.5 M) permitted the recovery of the greatest amount of antigen. Furthermore, it was most effective for recovery of immunodiffusion antigen from spleens which yielded unsatisfactory concentrations of antigen by the conventional freeze-thaw or water-extraction methods. The reactivity of the antigen did not appear to be affected by this chemical treatment.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis / Q000302:isolation & purification*; D004859:Equine Infectious Anemia / Q000276:immunology*; D005069:Evaluation Studies as Topic; D005615:Freezing; D006736:Horses; D005779:Immunodiffusion; D007158:Immunologic Techniques; D004860:Infectious Anemia Virus, Equine / Q000276:immunology*; D008722:Methods; D013154:Spleen / Q000276:immunology; D013861:Thiocyanates
D000956:Antigens, Viral; D013861:Thiocyanates