Translation in vitro of total nuclear RNA from HeLa cell nuclei infected with adenovirus 2.
Total heterogeneous nuclear RNA from HeLa cells infected with adenovirus for 18-20 hr stimulates amino acid incorporation into protein in a cell-free system from Ehrlich ascites tumors. This stimulation of protein synthesis by the nuclear RNA requires intact nuclei. The role of nuclei in this system is unknown, but evidence is presented that the nuclei are involved in the conversion of high-molecular-weight RNA to low-molecular-weight species. Some of the newly synthesized polypeptides appear to resemble the virion polypeptides.
D000256:Adenoviridae / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006367:HeLa Cells / Q000378:metabolism*; D006801:Humans; D007930:Leucine / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D012313:RNA / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis; D013539:Simian virus 40; D014316:Tritium; D014764:Viral Proteins / Q000096:biosynthesis
D002250:Carbon Radioisotopes; D012334:RNA, Neoplasm; D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium; D012313:RNA; D008715:Methionine; D007930:Leucine