Phosphorylation of high-molecular-weight membrane protein species in Chinese-hamster ovary cells in culture: effect of 6-N,2'-O-dibutyryladenosine 3':5'-cyclic monophosphate plus testosterone.
Growth of Chinese-hamster ovary cells in [(32)P]phosphate and [(3)H]leucine and subsequent assay of the plasma membranes reveals phosphorylation in two protein regions corresponding to molecular weights of 280000 and 195000. Culture in the presence of the 6-N,2-O'-dibutyryl derivative of cyclic AMP plus testosterone does not stimulate [(32)P]-phosphate incorporation, but determines a modification in the qualitative pattern of phosphorylation.
D000818:Animals; D002087:Butyrates / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D000242:Cyclic AMP / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007930:Leucine / Q000378:metabolism; D008970:Molecular Weight; D010053:Ovary / Q000187:drug effects / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D010750:Phosphoproteins / Q000096:biosynthesis*; D010759:Phosphorus Isotopes; D012995:Solubility; D013739:Testosterone / Q000494:pharmacology*; D013997:Time Factors; D014316:Tritium
D002087:Butyrates; D010710:Phosphates; D010750:Phosphoproteins; D010759:Phosphorus Isotopes; D014316:Tritium; D013739:Testosterone; D000242:Cyclic AMP; D007930:Leucine