Differences in membrane fluidity and structure in contact-inhibited and transformed cells.
Studies on contact-inhibited mouse embryo fibroblast 3T3 cells and 3T3 cells transformed by oncogenic RNA and DNA viruses and by a chemical carcinogen have demonstrated differences in plasma membrane architecture. Spin-label and freeze-fracture ultrastructural studies have shown that contact-inhibited cells have ordered membrane lipids and aggregated intramembranous particles, whereas transformed cells have fluid membrane lipids and randomly distributed intramembranous particles. These findings suggest a model for how changes in the cell membrane may account for some of the characteristic differences observed between contact-inhibited and transformed cells.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane; D002471:Cell Transformation, Neoplastic; D055598:Chemical Phenomena; D002621:Chemistry; D002999:Clone Cells; D003260:Contact Inhibition; D005347:Fibroblasts; D005613:Freeze Etching; D017988:Gammaretrovirus; D008055:Lipids; D008748:Methylcholanthrene; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D011120:Polyomavirus; D013539:Simian virus 40; D013113:Spin Labels
D008055:Lipids; D013113:Spin Labels; D008748:Methylcholanthrene