Mutagenic DNA polymerase in human leukemic cells.
Evidence is presented that DNA polymerases from human leukemic cells are mutagenic. Nucleic acid-free extracts of acute lymphoblastic leukemic cells polymerized about 10-times more dCTP using poly(dA-dT).poly(dA-dT) as a template than did extracts from normal lymphocytes. Mutagenic DNA polymerases could perhaps play an important role in tumor progression.
D000255:Adenosine Triphosphate / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D003597:Cytosine Nucleotides; D004254:DNA Nucleotidyltransferases / Q000097:blood*; D004261:DNA Replication; D004273:DNA, Neoplasm / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D006801:Humans; D007945:Leukemia, Lymphoid / Q000201:enzymology*; D008214:Lymphocytes / Q000201:enzymology; D009154:Mutation; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D010759:Phosphorus Isotopes; D011119:Polynucleotides / Q000378:metabolism; D013698:Templates, Genetic; D013942:Thymine Nucleotides / Q000378:metabolism; D014316:Tritium
D003597:Cytosine Nucleotides; D004273:DNA, Neoplasm; D010759:Phosphorus Isotopes; D011119:Polynucleotides; D013942:Thymine Nucleotides; D014316:Tritium; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D004254:DNA Nucleotidyltransferases; D010727:Phosphoric Diester Hydrolases