Discrimination between messenger ribonucleic acids by a mammalian translation initiation factor.
A factor from rabbit-reticulocyte ribosome, which stimulates by 5-fold initiation of hemoglobin mRNA translation in Krebs ascites cell extracts, was purified to homogeneity. The factor, a protein of about 65,000 molecular weight in sodium dodecyl sulfate, discriminates between various mRNAs: it stimulates translation of alpha globin mRNA and of tobacco mosaic virus RNA, but has only a small effect on beta globin mRNA and no effect at all on Mengo virus RNA translation.
D000818:Animals; D002287:Carcinoma, Krebs 2; D002460:Cell Line; D002474:Cell-Free System; D002848:Chromatography, DEAE-Cellulose; D005914:Globins / Q000096:biosynthesis; D006454:Hemoglobins; D007700:Kinetics; D008574:Mengovirus; D008715:Methionine / Q000378:metabolism; D051379:Mice; D010448:Peptide Initiation Factors / Q000302:isolation & purification; D011132:Polyribosomes / Q000032:analysis; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism; D011817:Rabbits; D012156:Reticulocytes / Q000032:analysis; D013460:Sulfur Isotopes; D014233:Tricarboxylic Acids / Q000494:pharmacology
D006454:Hemoglobins; D010448:Peptide Initiation Factors; D012333:RNA, Messenger; D012367:RNA, Viral; D013460:Sulfur Isotopes; D014233:Tricarboxylic Acids; D005914:Globins; D008715:Methionine