Concordant segregation of the expression of SV40 T antigen and human chromosome 7 in mouse-human hybrid subclones.
Subcloning of Simian virus 40 (SV40) T antigen-positive mouse-human hybrids, derived from the fusion of mouse cells deficient in thymidine kinase with SV40-transformed Lesch Nyhan fibroblasts, resulted in their segregation into T antigen-positive and negative subclones. Positive correlation between the presence of human chromosome 7 and the expression of SV40 T antigen was established in the subclones examined. These results negate the possibility of a transfer of the SV40 genome to a mouse chromosome.
D000818:Animals; D000956:Antigens, Viral; D002471:Cell Transformation, Neoplastic; D002906:Chromosomes, Human, 6-12 and X; D002999:Clone Cells; D005838:Genotype; D006801:Humans; D006822:Hybrid Cells / Q000378:metabolism*; D007621:Karyotyping; D051379:Mice; D013539:Simian virus 40 / Q000276:immunology*
D000956:Antigens, Viral