Separation of Herpes simplex virus-induced antigens by Concanavalin A affinity chromatography.
Biologically active herpes simplex virus (HSV)-induced antigens were selectively removed from extracts of infected BHK cells by affinity chromatography by utilizing an insoluble form of concanavalin A (Con A). Soluble extracts of (3)H-glucosamine-labeled, HSV-infected cells were absorbed to a Con A column. Bound material was eluted with alpha-methyl-d-mannoside (alphaMM) and NaCl. The specific activity of the eluted glycoproteins increased by 10-fold. Two broad groups of viral-induced antigens were isolated from Con A. Group I includes two antigens which bind to Con A by a specific mechanism because the antigens are dissociated by alphaMM. Group II contains three antigens which bind to Con A but apparently by a nonspecific or electrolytic mechanism. One antigen in group I was identified as the glycoprotein antigen, CP-1, described previously.
D000818:Animals; D000831:Animals, Newborn; D000956:Antigens, Viral / Q000302:isolation & purification*; D001322:Autoanalysis; D001667:Binding, Competitive; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D002623:Chemistry Techniques, Analytical; D002846:Chromatography, Affinity; D003208:Concanavalin A; D006224:Cricetinae; D003902:Detergents; D006023:Glycoproteins; D007106:Immune Sera / Q000032:analysis; D005779:Immunodiffusion / Q000295:instrumentation; D007553:Isotope Labeling; D007668:Kidney; D018139:Simplexvirus; D014316:Tritium; D010948:Viral Plaque Assay
D000956:Antigens, Viral; D002250:Carbon Radioisotopes; D003902:Detergents; D006023:Glycoproteins; D007106:Immune Sera; D014316:Tritium; D003208:Concanavalin A