Chromosome assignment of the T-antigen gene of simian virus 40 in African green monkey cells transformed by adeno 7-SV40 hybrid.
Somatic cell hybrids between mouse cells deficient in thymidine kinase [ATP:thymidine 5'-phosphotransferase (EC 2.7.1.75)] and two different monkey cell lines transformed by an adeno 7-SV40 hybrid have been produced using both a semiselective and a double selective procedure. Concordant segregation of the expression of SV40 T antigen with a specific monkey chromosome has been observed in all the mouse-monkey hybrid clones examined. Subcloning of three SV40 T antigen positive hybrid clones resulted in their segregation into SV40 T antigen-positive and negative subclones. Positive correlation between the SV40 T antigen and the same monkey chromosome has been observed in all the subclones examined.
D000256:Adenoviridae; D000818:Animals; D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D002459:Cell Fusion; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002874:Chromosome Mapping; D005796:Genes; D005838:Genotype; D000882:Haplorhini; D006822:Hybrid Cells; D006824:Hybridization, Genetic; D007621:Karyotyping; D051379:Mice; D010641:Phenotype; D013539:Simian virus 40; D013937:Thymidine Kinase / Q000172:deficiency
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D013937:Thymidine Kinase