Subcellular localization of hexadecanedioic acid activation in human liver.
The activation of hexadecanedioic acid has been studied in subcellular fractions of human liver. The activation capacity in a total homogenate of human liver was found to be 0.5 micro mole/min/g wet wt of tissue, about 10% of that for palmitic acid. Hexadecanedioic acid was activated by the mitochondrial and microsomal fractions. The mitochondrial enzyme is probably localized outside the inner mitochondrial compartment. The subcellular distribution of the hexadecanedioic acid activation was almost identical with the distribution of palmitic acid activation. Hexadecanedioic and palmitic acids seemed to compete for the same enzyme.
D000135:Acid Phosphatase / Q000032:analysis; D000217:Acyltransferases / Q000378:metabolism; D000328:Adult; D000368:Aged; D002331:Carnitine; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000378:metabolism; D002855:Chromatography, Thin Layer; D003593:Cytoplasm / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D005260:Female; D005952:Glucose-6-Phosphatase / Q000032:analysis; D006801:Humans; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism; D008995:Monoamine Oxidase / Q000032:analysis; D010169:Palmitic Acids / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology; D013347:Subcellular Fractions / Q000201:enzymology / Q000378:metabolism; D014316:Tritium
D005231:Fatty Acids, Unsaturated; D010169:Palmitic Acids; D014316:Tritium; D011092:Polyethylene Glycols; D008995:Monoamine Oxidase; D000217:Acyltransferases; D000135:Acid Phosphatase; D005952:Glucose-6-Phosphatase; D002331:Carnitine