Regulation by dietary choline of hepatic fatty acid synthetase in the rat.
Fatty acid synthetase activity was measured in the high-speed supernatant fraction of liver homogenates from rats fed a semisynthetic diet low in lipotropic factors. If choline was omitted from the diet, a significant increase of fatty acid synthetase activity was observed after two feedings of the deficient diet. Compared with controls, the increase of fatty acid synthetase activity was of a magnitude that could account for the amount of triglyceride accumulating in the hepatic floating lipid fraction. Gas-liquid chromatographic analysis of the floating lipid triglycerides showed an increased content of palmitic acid due to choline deficiency; this increase could be predicted from the increased fatty acid synthetase activity and its known characteristic yield of palmitic acid.
D000284:Administration, Oral; D000818:Animals; D001835:Body Weight; D002794:Choline / Q000008:administration & dosage / Q000494:pharmacology*; D002796:Choline Deficiency / Q000201:enzymology; D002845:Chromatography; D002849:Chromatography, Gas; D064429:Fatty Acid Synthases / Q000378:metabolism*; D008099:Liver / Q000032:analysis / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D010743:Phospholipids / Q000097:blood / Q000378:metabolism; D051381:Rats; D012822:Silicon Dioxide; D013997:Time Factors; D014280:Triglycerides / Q000032:analysis / Q000097:blood / Q000378:metabolism
D010743:Phospholipids; D014280:Triglycerides; D012822:Silicon Dioxide; D064429:Fatty Acid Synthases; D002794:Choline