title: Ultraviolet-sensitive mutator mutU4 of Escherichia coli inviable with polA.
abstract: Attempts to transduce the ultraviolet-sensitive mutator lesion mutU4 into strains deficient in deoxyribonucleic acid polymerase I (polA) were unsuccessful. Mutator recombinants were found when the polA recipient had first been reverted to Pol(+) by selection for resistance to methyl methanesulfonate. The inviability of the mutU4 polA double mutant was demonstrated by a reduction in the absolute number of transductants when the recipient was polA as compared with Pol(+), and selection was made for markers very close to mutU4. Double mutants containing mutU4 and polA4, which determines a cold-sensitive polymerase, were unable to grow at 24 C, the nonpermissive temperature.
mesh_terms: D000476:Alkanesulfonates / Q000494:pharmacology; D002470:Cell Survival; D004254:DNA Nucleotidyltransferases / Q000096:biosynthesis; D004260:DNA Repair; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D005804:Genes, Lethal; D009153:Mutagens; D009154:Mutation; D011830:Radiation Effects; D011995:Recombination, Genetic; D013696:Temperature; D014161:Transduction, Genetic; D014466:Ultraviolet Rays
chemical_list: D000476:Alkanesulfonates; D009153:Mutagens; D004254:DNA Nucleotidyltransferases