Immunochemical procedure for partial purification of newly synthesized proteins in polyoma virus-infected mouse cells.
A broad-spectrum antiserum has been obtained against nuclear proteins from normal mouse fibroblasts. Solid phase immunoadsorbants prepared from the serum specifically bind the corresponding antigens. The removal in this way of a significant fraction of host proteins leads to partial purification of viral and virus-induced polypeptides from polyoma virus-infected cell extracts. Four main peptides can be selected, with respective molecular weights of 90,000, 70,000, 46,000, and 41,000.
D000327:Adsorption; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D000906:Antibodies; D001345:Autoradiography; D002250:Carbon Radioisotopes; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D007120:Immunochemistry; D005779:Immunodiffusion; D008722:Methods; D051379:Mice; D008815:Mice, Inbred Strains / Q000196:embryology; D008858:Microscopy, Phase-Contrast; D008970:Molecular Weight; D009698:Nucleoproteins; D010455:Peptides / Q000032:analysis; D011120:Polyomavirus / Q000032:analysis* / Q000378:metabolism; D012756:Sheep / Q000276:immunology; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification*
D000596:Amino Acids; D000906:Antibodies; D002250:Carbon Radioisotopes; D007106:Immune Sera; D009698:Nucleoproteins; D010455:Peptides; D014764:Viral Proteins