Restoration of the conversion of desmosterol to cholesterol in L-cells after hybridization with human fibroblasts.
Hybrids between different human cells (which synthesize cholesterol) and mouse cells (whose end-product of sterol synthesis is desmosterol) were analyzed for the ability to convert desmosterol to cholesterol. Conversion of [(14)C]desmosterol to cholesterol and incorporation of [(14)C]acetate into the end-product sterol were studied in the parental and hybrid cells. Concordant segregation of the conversion of desmosterol to cholesterol and the human chromosome F-20 was observed.
D000085:Acetates / Q000378:metabolism; D000818:Animals; D002250:Carbon Radioisotopes; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002784:Cholesterol / Q000096:biosynthesis*; D002874:Chromosome Mapping; D002903:Chromosomes, Human, 19-20; D003897:Desmosterol / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts; D005796:Genes; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology*; D007621:Karyotyping; D007739:L Cells / Q000201:enzymology; D007926:Lesch-Nyhan Syndrome; D008297:Male; D051379:Mice; D012730:Sex Chromosomes; D013539:Simian virus 40; D013250:Steroid Hydroxylases / Q000096:biosynthesis* / Q000378:metabolism
D000085:Acetates; D002250:Carbon Radioisotopes; D003897:Desmosterol; D002784:Cholesterol; D013250:Steroid Hydroxylases