Evidence for modification of protein phosphorylation by cytokinins.
Kinetin stimulated phosphorylation of protein in floated Chinese-cabbage leaf discs, but inhibited protein phosphorylation in nuclei+chloroplast extracts from Chinese-cabbage or tobacco leaves. Kinetin also inhibited protein phosphorylation in isolated tobacco nuclei or nuclei from carrot secondary-phloem tissue. Purified Chinese-cabbage leaf ribosomes exhibited protein kinase activity which was inhibited by kinetin and zeatin. The ribosome-associated kinase responded to kinetin and zeatin differently from that associated with nuclei+chloroplast preparations. Protein phosphorylation in vitro was not affected by adenosine 3':5'-cyclic monophosphate, indol-3-ylacetic acid or gibberellic acid. It was only inhibited by N(9)-unsubstituted purines, among which the known cytokinins were the most effective inhibitors. The results are discussed in relation to possible similarities between the effects of cytokinins in plant tissues and the effects of adenosine 3':5'-cyclic monophosphate in animal tissues. Both compounds appear to modify the activity of protein kinases and both affect many different cellular processes.
D000225:Adenine / Q000494:pharmacology*; D000605:Amino Alcohols / Q000494:pharmacology; D002467:Cell Nucleus / Q000378:metabolism; D002474:Cell-Free System; D002736:Chloroplasts / Q000378:metabolism; D000242:Cyclic AMP / Q000494:pharmacology; D003864:Depression, Chemical; D005875:Gibberellins / Q000494:pharmacology; D007210:Indoleacetic Acids / Q000494:pharmacology; D008934:Mitogens / Q000494:pharmacology*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010759:Phosphorus Isotopes; D010770:Phosphotransferases / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000378:metabolism*; D010944:Plants / Q000378:metabolism; D010947:Plants, Toxic; D011687:Purines / Q000494:pharmacology; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism; D013268:Stimulation, Chemical; D014026:Nicotiana
D000605:Amino Alcohols; D005875:Gibberellins; D007210:Indoleacetic Acids; D008934:Mitogens; D010759:Phosphorus Isotopes; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011687:Purines; D000242:Cyclic AMP; D010770:Phosphotransferases; D000225:Adenine