Analysis of simian virus 40 DNA with the restriction enzyme of Haemophilus aegyptius, endonuclease Z.
Limited digestion of simian virus 40 (SV40) DNA from both small- and large- plaque strains with the restriction endonuclease Z from Haemophilus aegyptius yielded 10 specific fragments. The number of nucleotide pairs for each fragment, determined by co-electrophoresis with phiX174 RF fragments produced by endonuclease Z, ranges from 2,050 to 80. The difference in the pattern between the large- and small-plaque strains is the disappearance of one fragment containing approximately 255 nucleotide pairs and the appearance of a new fragment with 145 nucleotide pairs. This finding can be explained either by deletions or insertions totaling 110 nucleotide pairs. Complementary RNA synthesized in vitro from the adeno-SV40 hybrid virus, strain ND-1, hybridized preferentially to four of the fragments of SV40 DNA.
D000256:Adenoviridae; D000818:Animals; D001345:Autoradiography; D002247:Carbon Isotopes; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases / Q000302:isolation & purification / Q000378:metabolism*; D006190:Haemophilus / Q000201:enzymology*; D000882:Haplorhini; D007668:Kidney; D009693:Nucleic Acid Hybridization; D009711:Nucleotides / Q000032:analysis; D010759:Phosphorus Isotopes; D013539:Simian virus 40 / Q000032:analysis* / Q000254:growth & development; D014316:Tritium
D002247:Carbon Isotopes; D004279:DNA, Viral; D009711:Nucleotides; D010759:Phosphorus Isotopes; D014316:Tritium; D004720:Endonucleases