Determination of the molecular weight of animal RNA viral genomes by nuclease digestions. I. Vesicular stomatitis virus and its defective T particle.
A procedure has been developed for the determination of the weight of animal RNA virus genomes using controlled nuclease digestions and computation of the moles of oligonucleotides obtained from 1 mol of RNA. Using both pancreatic RNase and RNase T(1) to digest viral RNA labeled by (3)H-uridine, (3)H-cytidine, or (3)H-guanosine, the weight of the virion RNA of vesicular stomatitis virus (VSV) is estimated as 3.82 +/- 0.14 x 10(6) whereas that of the VSV-defective T particle is estimated as 1.23 +/- 0.04 x 10(6).
D000818:Animals; D002460:Cell Line; D002848:Chromatography, DEAE-Cellulose; D006224:Cricetinae; D003562:Cytidine / Q000378:metabolism; D003673:Defective Viruses / Q000032:analysis*; D006151:Guanosine / Q000378:metabolism; D006868:Hydrolysis; D007553:Isotope Labeling; D007668:Kidney; D008722:Methods; D008970:Molecular Weight; D009841:Oligonucleotides / Q000032:analysis; D010179:Pancreas / Q000201:enzymology; D010761:Phosphorus Radioisotopes; D012367:RNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D012260:Ribonucleases; D013997:Time Factors; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D014721:Vesicular stomatitis Indiana virus / Q000032:analysis* / Q000254:growth & development
D009841:Oligonucleotides; D010761:Phosphorus Radioisotopes; D012367:RNA, Viral; D014316:Tritium; D006151:Guanosine; D003562:Cytidine; D012260:Ribonucleases; D014529:Uridine