title: Mechanism of interferon action: inhibition of viral messenger ribonucleic acid translation in L-cell extracts.
abstract: Encephalomyocarditis (EMC) virus ribonucleic acid (RNA) stimulated the incorporation of (14)C-amino acids into polypeptides in cell-free systems using preincubated S10 extracts from L cells. Incorporation was linear for over 2 hr. Analysis of the tryptic peptides derived from the polypeptide products formed in response to EMC RNA showed them to be virus specific. The major product, a polypeptide of 140,000 in molecular weight, migrated on sodium dodecyl sulfate-polyacrylamide gels with one of the virus-specific polypeptides present in EMC-infected cells. A minor component of molecular weight about 230,000 may correspond to the product of complete translation of the EMC virus genome. Little or no effect of interferon or vaccinia virus infection was observed in the preincubated, cell-free system. The EMC RNA-stimulated incorporation of (14)C-amino acids into polypeptides was not inhibited in extracts derived from L cells early in virus infection, from interferon-treated cells, or from cells subjected to both treatments. Interferon treatment did appear to have a slight inhibitory effect on chain elongation in this system. However, treatment of cells with highly purified interferon before virus infection caused a decrease of about 80% in the capacity of non-preincubated cell extracts to translate added EMC RNA. This effect did not extend to the translation of polyuridylic acid and could be reversed by preincubation of the extracts at 37 C for 20 min. The inhibition of translation was manifest at interferon concentrations as low as 5IU/ml, and in this respect closely paralleled the inhibition of virus growth. Inactivation of the antiviral activity of the interferon by heating or digestion with trypsin also abolished the effect on cell-free protein synthesis. The EMC-specific polypeptides formed in reduced amounts in extracts of interferon-treated vaccinia-infected cells were smaller than those formed in extracts of untreated, vaccinia-infected cells. Thus, inhibition of initiation or elongation of polypeptides, or both, can be demonstrated in cell-free systems employing non-preincubated extracts from interferon-treated, virus-infected cells. These results indicate that antiviral activity of interferon is directed against the translation of viral messenger RNA.
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002247:Carbon Isotopes; D002474:Cell-Free System; D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D004686:Encephalomyocarditis virus / Q000032:analysis / Q000254:growth & development / Q000378:metabolism*; D007372:Interferons / Q000494:pharmacology*; D007739:L Cells; D008715:Methionine / Q000378:metabolism; D051379:Mice; D010452:Peptide Biosynthesis; D010441:Peptide Chain Elongation, Translational / Q000187:drug effects; D010455:Peptides / Q000032:analysis; D010759:Phosphorus Isotopes; D014176:Protein Biosynthesis / Q000187:drug effects*; D012333:RNA, Messenger; D012367:RNA, Viral / Q000494:pharmacology; D013268:Stimulation, Chemical; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis
chemical_list: D000596:Amino Acids; D002247:Carbon Isotopes; D010455:Peptides; D010759:Phosphorus Isotopes; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D007372:Interferons; D008715:Methionine