Induction of virus synthesis in polyoma-transformed BHK-21 cells.
BHK-21 cells were transformed with polyoma virus mutants Ts-a and Ts-25 by using a temperature shift from 31 to 39 C at 5 days after infection so that rescuable transformants could be isolated. Clones which yielded virus after fusion with mouse cells were scored and maintained at 39 C in the presence of antipolyoma virus antiserum. Generally, no infectious viral deoxyribonucleic acid (DNA) could be found in Hirt supernatant fractions of these lines when maintained at 39 C, but DNA-DNA reannealing measurements detected two to six viral genomes per diploid cell genome in the nuclear DNA. Fusion with permissive cells was not necessary to induce the synthesis of infectious virus; cell lines shifted to 31 C produce the equivalent of 100 viral genomes per cell after 5 days. In some cell lines up to 1% of the cells formed infectious centers upon a shift to 31 C, and 100% of the subclones of a line were inducible. Growth at 31 C selected for a noninducible population which was still transformed.
D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification; D002459:Cell Fusion; D002460:Cell Line / Q000382:microbiology*; D002471:Cell Transformation, Neoplastic; D011232:Chemical Precipitation; D006224:Cricetinae; D004261:DNA Replication; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D007668:Kidney; D051379:Mice; D009154:Mutation; D010759:Phosphorus Isotopes; D011120:Polyomavirus / Q000032:analysis / Q000254:growth & development* / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D013696:Temperature; D014461:Ultracentrifugation; D014779:Virus Replication
D000956:Antigens, Viral; D004279:DNA, Viral; D010759:Phosphorus Isotopes