title: Relationship of replication and transcription of Simian Virus 40 DNA.
abstract: RNA produced by the Simian Virus 40 (SV40) mutant tsA30 during lytic infection of kidney cells of African green monkeys was examined by RNA-DNA competition-hybridization. This mutant is temperature-sensitive in a function (gene A) that regulates synthesis of viral DNA. No detectable difference between mutant RNA synthesized at the permissive temperature (33 degrees ) and wild-type viral RNA was found. During continuous infection with the mutant at the restrictive temperature (41 degrees ) only early viral RNA was produced. When mutant DNA and late RNA synthesis were initiated at the permissive temperature, a shift to the restrictive temperature rapidly terminated synthesis of viral DNA but not that of late viral RNA. The data indicate that the function of gene A is required before synthesis of late viral RNA and that after initiation, the production of late RNA continues without further expression of gene A or concomittant viral DNA synthesis.
mesh_terms: D000818:Animals; D002247:Carbon Isotopes; D002460:Cell Line; D004261:DNA Replication; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis*; D000882:Haplorhini; D007668:Kidney; D008970:Molecular Weight; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010759:Phosphorus Isotopes; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis; D013539:Simian virus 40 / Q000378:metabolism*; D013696:Temperature; D013936:Thymidine / Q000378:metabolism; D014158:Transcription, Genetic; D014316:Tritium; D014529:Uridine / Q000378:metabolism
chemical_list: D002247:Carbon Isotopes; D004279:DNA, Viral; D010759:Phosphorus Isotopes; D012367:RNA, Viral; D014316:Tritium; D013936:Thymidine; D014529:Uridine