Latent DNA of Epstein-Barr virus: separation from high-molecular-weight cell DNA in a neutral glycerol gradient.
Epstein-Barr virus DNA in virus non-productive cells was separated from high-molecular-weight cell DNA by sedimentation through a neutral glycerol gradient after gentle lysis of cells by Pronase and Sarkosyl. The isolated Epstein-Barr virus DNA had a density of 1.716-1.717 g/cm(3) in CsCl equilibrium centrifugation, which is very close to the virus DNA density of 1.718 g/cm(3). The results indicated that the majority of Epstein-Barr virus DNA in virus nonproductive cells is not covalently integrated into high-molecular-weight cell DNA.
D002051:Burkitt Lymphoma; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D004273:DNA, Neoplasm / Q000032:analysis*; D004279:DNA, Viral / Q000302:isolation & purification*; D005990:Glycerol; D004854:Herpesvirus 4, Human / Q000032:analysis*; D006801:Humans; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral
D004273:DNA, Neoplasm; D004279:DNA, Viral; D012367:RNA, Viral; D005990:Glycerol