Cytomegalovirus: conversion of nonpermissive cells to a permissive state for virus replication.
Human embryonic kidney cells are epithelioid cells which are normally nonpermissive for in vitro replication of human cytomegalovirus. These cells were converted to a permissive state for the virus by prior treatment with 5-iodo-2'-deoxyuridine. When this method was used, a nonpermissive cell was made permissive to an infecting virus.
D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000254:growth & development* / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D004279:DNA, Viral / Q000096:biosynthesis; D004622:Embryo, Mammalian; D004847:Epithelial Cells; D006801:Humans; D007065:Idoxuridine / Q000494:pharmacology*; D007668:Kidney; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000187:drug effects*
D004279:DNA, Viral; D014316:Tritium; D004247:DNA; D007065:Idoxuridine; D013936:Thymidine