title: Salmonella typhimurium mutants defective in cytidine monophosphate kinase (cmk).
abstract: Mutants of Salmonella typhimurium defective in cytidine 5'-monophosphate (CMP) kinase (cmk) have been isolated. The mutants also lack the ability to phosphorylate 2'-deoxyCMP, indicating that one enzyme is responsible for the phosphorylation of both CMP and deoxyCMP to the corresponding diphosphates. In glucose minimal medium the mutants grow at the same rate as the parental strain; however, they excrete large quantities of pyrimidines into the growth medium. Cytidine but not deoxycytidine has been identified among the excreted products. The mutant phenotype suggests that the physiological role of CMP kinase is that of rephosphorylating CMP arising from the breakdown of messenger ribonucleic acid. This proposed role of CMP kinase is supported by the fact that a cmk(-) mutant is much more sensitive to any partial impairment of cytidine 5'-triphosphate synthetase than is the cmk(+) parent strain. The gene cmk has been located on the Salmonella chromosome at 38.5 min. No markers which can be cotransduced with cmk by phage P22 have been found.
mesh_terms: D001345:Autoradiography; D002250:Carbon Radioisotopes; D002874:Chromosome Mapping; D003562:Cytidine / Q000378:metabolism; D003597:Cytosine Nucleotides / Q000378:metabolism; D003843:Deoxycytidine Monophosphate / Q000378:metabolism; D005461:Fluorine; D005796:Genes; D009153:Mutagens; D009154:Mutation; D009604:Nitrosoguanidines; D010085:Oxidative Phosphorylation; D010761:Phosphorus Radioisotopes; D010770:Phosphotransferases / Q000096:biosynthesis* / Q000378:metabolism; D012486:Salmonella typhimurium / Q000201:enzymology*; D014161:Transduction, Genetic; D014364:Tryptophan / Q000378:metabolism; D014466:Ultraviolet Rays; D014529:Uridine / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D002250:Carbon Radioisotopes; D003597:Cytosine Nucleotides; D009153:Mutagens; D009604:Nitrosoguanidines; D010761:Phosphorus Radioisotopes; D003843:Deoxycytidine Monophosphate; D005461:Fluorine; D003562:Cytidine; D014364:Tryptophan; D010770:Phosphotransferases; D014529:Uridine