Active transport of exogenous S-adenosylmethionine and related compounds into cells and vacuoles of Saccharomyces cerevisiae.
Saccharomyces cerevisiae 4094-B (alpha, ade-2, ura-1) in potassium phosphate buffer with glucose under aerobic conditions took up (-)S-adenosyl-l-methionine from the medium in sufficient quantity to permit the demonstration of its accumulation in the vacuole by ultraviolet micrography. The same result was obtained with (+/-)S-adenosyl-l-methionine, (+/-)S-adenosyl-d-methionine, and (-)S-adenosyl-l-ethionine. The rate of uptake was slow with (-)S-adenosyl-S(n-propyl)-l-homocysteine and S-adenosyl-d-homocysteine. S-Adenosyl-l-homocysteine was assimilated rapidly, but intracellular degradation precluded accumulation and ultraviolet micrographic studies. The uptake of 5'-methyl-, 5'-ethyl-, 5'-n-propylthioadenosine, and 5'-dimethylsulfonium adenosine was minimal.
D000332:Aerobiosis; D001693:Biological Transport, Active; D002021:Buffers; D002250:Carbon Radioisotopes; D005001:Ethionine / Q000031:analogs & derivatives / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006710:Homocysteine / Q000031:analogs & derivatives / Q000378:metabolism; D002479:Inclusion Bodies / Q000378:metabolism*; D008860:Microscopy, Ultraviolet; D010710:Phosphates; D012436:S-Adenosylmethionine / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D013237:Stereoisomerism; D014617:Vacuoles / Q000378:metabolism*
D002021:Buffers; D002250:Carbon Radioisotopes; D010710:Phosphates; D006710:Homocysteine; D012436:S-Adenosylmethionine; D005947:Glucose; D005001:Ethionine