An RNA-dependent nucleoside triphosphate phosphohydrolase (ATPase) associated with rho termination factor.
Highly purified rho termination factor from Escherichia coli catalyzes an RNA-dependent hydrolysis of ribonucleoside triphosphates to nucleoside diphosphates and inorganic phosphate. In the presence of poly(C), a specific activity of 100 mumole of ATP hydrolyzed per min/mg has been measured. The phosphohydrolase activity appears to be associated with the protein responsible for termination of RNA synthesis, but a functional relationship between the two activities is not yet evident. Hydrolysis of nucleoside triphosphates occurs in the absence of termination and without any extensive degradation of RNA.
D000251:Adenosine Triphosphatases / Q000302:isolation & purification; D000255:Adenosine Triphosphate; D002250:Carbon Radioisotopes; D002384:Catalysis; D002712:Chlorides; D002845:Chromatography; D003562:Cytidine; D012321:DNA-Directed RNA Polymerases; D004926:Escherichia coli / Q000201:enzymology*; D006358:Hot Temperature; D008274:Magnesium; D009841:Oligonucleotides; D010454:Peptide Termination Factors; D010744:Phosphoric Monoester Hydrolases; D012367:RNA, Viral; D012260:Ribonucleases; D014529:Uridine
D002250:Carbon Radioisotopes; D002712:Chlorides; D009841:Oligonucleotides; D010454:Peptide Termination Factors; D012367:RNA, Viral; D003562:Cytidine; D000255:Adenosine Triphosphate; D012321:DNA-Directed RNA Polymerases; D012260:Ribonucleases; D010744:Phosphoric Monoester Hydrolases; D000251:Adenosine Triphosphatases; D008274:Magnesium; D014529:Uridine