Nature of the scrapie agent: evidence against a viroid.
The postulated viroid nature of scrapie agent was tested. Since the agent could not be recovered after a carefully controlled phenol extraction of infected mouse brains, it is suggested that this agent is not a viroid.
D000818:Animals; D001921:Brain / Q000382:microbiology; D002250:Carbon Radioisotopes; D002499:Centrifugation, Density Gradient; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D008574:Mengovirus / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D010636:Phenols; D011328:Prions / Q000032:analysis / Q000472:pathogenicity; D012367:RNA, Viral / Q000032:analysis; D012997:Solvents; D013936:Thymidine; D014316:Tritium; D014529:Uridine
D002250:Carbon Radioisotopes; D004279:DNA, Viral; D010636:Phenols; D011328:Prions; D012367:RNA, Viral; D012997:Solvents; D014316:Tritium; D013936:Thymidine; D014529:Uridine