Growth inhibition of murine tumor cells, in vitro, by puromycin, ( 6 N)O 2 '-dibutyryl 3',5'-adenosine monophosphate, or adenosine: evidence of commitment for cell division.
The cytostatic effects of puromycm, [(6)N]O(2')-dibutyryl 3',5'-adenosine monophosphate, and adenosine on asynchronous and synchronous cultures of the murine mastocytoma, P815Y, have been studied. Cell growth was arrested after a minimum of one further division. A model is proposed for the inhibition of cell division in which the periods of inhibition and growth arrest are separated in time by one cell cycle.
D000241:Adenosine / Q000494:pharmacology*; D000818:Animals; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D000242:Cyclic AMP / Q000494:pharmacology*; D003584:Cytological Techniques; D004273:DNA, Neoplasm / Q000032:analysis; D012515:Mast-Cell Sarcoma; D051379:Mice; D008954:Models, Biological; D011691:Puromycin / Q000494:pharmacology*; D013347:Subcellular Fractions / Q000032:analysis; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium
D004273:DNA, Neoplasm; D014316:Tritium; D011691:Puromycin; D000242:Cyclic AMP; D000241:Adenosine; D013936:Thymidine