Kinetic properties of the partially purified pyruvate dehydrogenase complex of ox brain.
The properties of a purified preparation of the pyruvate dehydrogenase complex from ox brain have been compared with those of a similar preparation from ox kidney. A broad pH optimum around 7.8, similar dependence on ionic strength, and independence of the nature of the buffer anions or cations characterized preparations from both tissues. Michaelis constants for the binding of pyruvate, thiamin pyrophosphate, NAD(+) and CoA were also similar. Enzyme from both tissues was inhibited by NADH, by copper and other heavy metals, by high concentrations of tricarboxylic acid-cycle intermediates, and by preincubation with ATP. Acetyl-CoA itself did not appear to inhibit these preparations, although some commercial preparations of acetyl-CoA did contain an inhibitor. Although oxaloacetate and alpha-oxobutyrate were weak inhibitors, a number of other alpha-oxo acids including phenylpyruvate did not inhibit. The properties of the pyruvate dehydrogenase complex from brain and kidney appeared similar.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002021:Buffers; D002247:Carbon Isotopes; D002417:Cattle; D002540:Cerebral Cortex / Q000166:cytology / Q000201:enzymology*; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D006863:Hydrogen-Ion Concentration; D007651:Keto Acids; D007668:Kidney / Q000166:cytology / Q000201:enzymology; D007700:Kinetics; D013058:Mass Spectrometry; D008670:Metals / Q000494:pharmacology; D008928:Mitochondria / Q000201:enzymology; D009097:Multienzyme Complexes / Q000378:metabolism*; D009243:NAD / Q000494:pharmacology; D009928:Organ Specificity; D009994:Osmolar Concentration; D011768:Pyruvate Dehydrogenase Complex / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism
D002021:Buffers; D002247:Carbon Isotopes; D007651:Keto Acids; D008670:Metals; D009097:Multienzyme Complexes; D011768:Pyruvate Dehydrogenase Complex; D009243:NAD; D000255:Adenosine Triphosphate