Introduction of specific cleavages into RNAs of RNA bacteriophages for determination of base sequences.
Large fragments of R17, MS2, and Qbeta RNAs were obtained reproducibly by limited digestion by ribonuclease T1. After digestion of R17 and MS2 RNAs, two pairs of large fragments were obtained that probably resulted from specific cleavages of the whole molecules. The cleavages of MS2 RNA were produced at points 36 and 47% from the 5' end.
D000255:Adenosine Triphosphate / Q000378:metabolism; D001345:Autoradiography; D001483:Base Sequence; D003090:Coliphages / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D010761:Phosphorus Radioisotopes; D010770:Phosphotransferases / Q000378:metabolism; D011119:Polynucleotides / Q000032:analysis; D012328:RNA Viruses / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis* / Q000378:metabolism; D012260:Ribonucleases / Q000037:antagonists & inhibitors / Q000378:metabolism; D012265:Ribonucleotides / Q000032:analysis*; D013045:Species Specificity; D013431:Sulfates / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology
D010761:Phosphorus Radioisotopes; D011119:Polynucleotides; D012367:RNA, Viral; D012265:Ribonucleotides; D013431:Sulfates; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D012260:Ribonucleases; D015032:Zinc