Reversible inhibition of poliovirus RNA synthesis in vivo and in vitro by viral products.
In poliovirus-infected HeLa-S3 cells, the protease inhibitors tolylsulfonyl-phenylalanyl chloromethyl ketone and iodoacetamide cause an accumulation of large precursor proteins, and they block viral RNA synthesis most probably via these products. Viral RNA polymerase activity can, however, be extracted by detergent containing buffer (Tris/Nonidet P-40, deoxycholate) from the inhibited cells. Only cytoplasmic extracts from infected cells treated with tolylsulfonyl-phenylalanyl chloromethyl ketone or iodoacetamide contain a protein which inhibits the in vitro polymerase reaction.
D000081:Acetamides / Q000494:pharmacology*; D002250:Carbon Radioisotopes; D003513:Cycloheximide / Q000494:pharmacology; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D003864:Depression, Chemical; D006367:HeLa Cells; D006801:Humans; D017955:Poliovirus / Q000201:enzymology / Q000378:metabolism*; D012367:RNA, Viral / Q000096:biosynthesis*; D013997:Time Factors; D014105:Tosyl Compounds / Q000494:pharmacology*; D014316:Tritium; D014361:Trypsin Inhibitors / Q000494:pharmacology*; D014500:Uracil Nucleotides / Q000378:metabolism; D014529:Uridine / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis
D000081:Acetamides; D002250:Carbon Radioisotopes; D012367:RNA, Viral; D014105:Tosyl Compounds; D014361:Trypsin Inhibitors; D014500:Uracil Nucleotides; D014764:Viral Proteins; D014316:Tritium; D003513:Cycloheximide; D012321:DNA-Directed RNA Polymerases; D014529:Uridine