Hydroxyapatite chromatography and formamide denaturation of adenovirus DNA.
Denatured adenovirus DNA was retained by hydroxyapatite columns under conditions generally used for selective retention of double-stranded DNA, probably due to several partially complementary sequences within single-stranded DNA. It was found that addition of formamide reduced the fraction of sonically treated, denatured adenovirus DNA bound to hydroxyapatite from about 30% to less than 1%. This led to a study of the effect of formamide on the melting temperature (T(m)) of double-stranded DNA in solution or bound to hydroxyapatite. The T(m) of DNA decreases 0.56 C/1% formamide, a value determined in buffered solutions with purified formamide.
D000256:Adenoviridae / Q000032:analysis*; D002250:Carbon Radioisotopes; D002499:Centrifugation, Density Gradient; D002845:Chromatography / Q000592:standards*; D004277:DNA, Single-Stranded / Q000032:analysis / Q000302:isolation & purification; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification*; D005559:Formamides; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D006882:Hydroxyapatites; D008722:Methods; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D009695:Nucleic Acid Renaturation; D013539:Simian virus 40 / Q000032:analysis; D013053:Spectrophotometry; D014316:Tritium; D014732:Vibration
D002250:Carbon Radioisotopes; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D005559:Formamides; D006882:Hydroxyapatites; D014316:Tritium