title: Methanol metabolism in pseudomonad C.
abstract: Cell suspensions of pseudomonad C, a bacterium capable of growth on methanol as sole carbon source, were able to oxidize methanol, formaldehyde, and formate, although the rates of oxidation for the latter two compounds were much slower. The latter compounds also could not serve as sole carbon sources. Through the use of labeled compounds, it was shown that in the presence of methanol, formaldehyde, formate, and bicarbonate were incorporated into trichloroacetic acid-precipitable material. Hexose phosphate synthetase activity was found, indicating the assimilation of methanol via an allulose pathway. No hydroxypyruvate reductase activity was found, nor was any complex membrane structure observed. Such a combination of characteristics has been observed in an obligate methylotroph (Pseudomonas W1), but pseudomonad C can utilize a variety of non-methyl substrates.
mesh_terms: D000429:Alcohol Oxidoreductases / Q000032:analysis; D001639:Bicarbonates / Q000378:metabolism; D002247:Carbon Isotopes; D003470:Culture Media; D004140:Dinitrophenols / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D005557:Formaldehyde / Q000378:metabolism; D005561:Formates / Q000378:metabolism; D006600:Hexosephosphates / Q000096:biosynthesis; D000432:Methanol / Q000378:metabolism*; D008854:Microscopy, Electron; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D010659:Phenylhydrazines / Q000494:pharmacology; D011549:Pseudomonas / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*
chemical_list: D001639:Bicarbonates; D002247:Carbon Isotopes; D003470:Culture Media; D004140:Dinitrophenols; D005561:Formates; D006600:Hexosephosphates; D010659:Phenylhydrazines; D009243:NAD; D005557:Formaldehyde; D004492:Edetic Acid; D000429:Alcohol Oxidoreductases; D000432:Methanol