Anthranilate synthase-anthranilate 5-phosphoribosyl 1-pyrophosphate phosphoribosyltransferase from Aerobacter aerogenes.
1. Anthranilate synthase and phosphoribosyltransferase from Aerobacter aerogenes purify simultaneously and sediment together on sucrose gradients, showing that they occur as an enzyme aggregate. Both activities of the intact aggregate are subject to inhibition by tryptophan. 2. By using appropriate auxotrophic mutants it was shown that an intact active enzyme aggregate is formed when the components come from separate mutant strains. An intact active aggregate can also be formed when one component is from Escherichia coli and the other from A. aerogenes. 3. Phosphoribosyltransferase of A. aerogenes is active when not in an aggregate with anthranilate synthase, but is not subject to tryptophan inhibition, indicating that the inhibitor site is on the anthranilate synthase component. 4. Anthranilate synthase can be active and sensitive to tryptophan inhibition when complexed with an inactive phosphoribosyltransferase. 5. Kinetic studies on the anthranilate synthase activity show that tryptophan is a competitive inhibitor with respect to chorismate and a non-competitive inhibitor with respect to either glutamine or NH(4) (+) ions. This is consistent with a sequential mechanism of the ordered type in which chorismate is the first reactant.
D001665:Binding Sites; D002499:Centrifugation, Density Gradient; D003509:Cyclohexanecarboxylic Acids; D004591:Electrophoresis, Polyacrylamide Gel; D004754:Enterobacter / Q000201:enzymology*; D004926:Escherichia coli / Q000201:enzymology; D005973:Glutamine / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009154:Mutation; D010428:Pentosephosphates; D010430:Pentosyltransferases / Q000378:metabolism; D010756:Phosphoric Acids; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D000637:Transaminases / Q000378:metabolism; D014364:Tryptophan / Q000494:pharmacology; D014753:Vinyl Compounds; D062367:ortho-Aminobenzoates
D003509:Cyclohexanecarboxylic Acids; D010428:Pentosephosphates; D010756:Phosphoric Acids; D000644:Quaternary Ammonium Compounds; D014753:Vinyl Compounds; D062367:ortho-Aminobenzoates; D005973:Glutamine; D014364:Tryptophan; D010430:Pentosyltransferases; D000637:Transaminases