In vitro radioisotopic labeling of proteins associated with purified polyoma virions.
An in vitro procedure using [(14)C]formaldehyde and sodium borohydride was used to label the proteins of purified polyoma virions. This method, which labeled the three capsid proteins and the four internal histones of polyoma virus, did not alter the biological and biophysical characteristics of the virus. In addition to the seven viral proteins, five additional radioactive peaks were resolved on polyacrylamide gels. Four of these peaks could be attributed to growing the virus in the presence of serum. The fifth peak appears to be a nonhistone host-contributed protein made before infection.
D002250:Carbon Radioisotopes; D004279:DNA, Viral / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D007553:Isotope Labeling / Q000379:methods*; D011120:Polyomavirus; D012967:Sodium Dodecyl Sulfate; D014764:Viral Proteins
D002250:Carbon Radioisotopes; D004279:DNA, Viral; D014764:Viral Proteins; D012967:Sodium Dodecyl Sulfate