A collagenase in extracts of the invertebrate Bipalium kewense.
The localization, isolation and partial characterization of a collagenolytic enzyme from the land planarian Bipalium kewense is described. This enzyme can be obtained by direct extraction of the organism, and can be separated from non-collagenous proteolytic activity by (NH(4))(2)SO(4) precipitation and Sephadex-gel chromatography. Its mode of attack on collagen and sensitivity to a variety of inhibitors indicate that this enzyme differs from vertebrate collagenases and a previously described invertebrate collagenase.
D000645:Ammonium Sulfate; D000818:Animals; D002247:Carbon Isotopes; D002268:Carboxypeptidases / Q000037:antagonists & inhibitors; D002474:Cell-Free System; D002850:Chromatography, Gel; D003094:Collagen; D004591:Electrophoresis, Polyacrylamide Gel; D006168:Guinea Pigs; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D003012:Microbial Collagenase / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D012867:Skin; D013053:Spectrophotometry; D013438:Sulfhydryl Compounds; D013997:Time Factors; D014419:Turbellaria / Q000201:enzymology*; D014783:Viscosity
D002247:Carbon Isotopes; D013438:Sulfhydryl Compounds; D003094:Collagen; D002268:Carboxypeptidases; D003012:Microbial Collagenase; D000645:Ammonium Sulfate