title: Isolation and characterization of revertant cell lines. VI. Susceptibility of revertants to retransformation by simian virus 40 and murine sarcoma virus.
abstract: The susceptibility of two classes of revertants of Simian virus 40 (SV40)-transformed 3T3 cells to retransformation by SV40 or murine sarcoma virus (MSV) was studied. Both serum-sensitive and density-sensitive revertants are not retransformable by SV40. MSV can transform both types of revertants. The MSV-transformed revertants grow to high cell densities and form colonies when suspended in semi-solid methylcellulose medium, but are unable to grow in 1% calf serum. The MSV-transformed revertants produce infectious MSV and murine leukemia virus and possess the same number of chromosomes as the untransformed revertants.
mesh_terms: D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002875:Chromosomes; D005347:Fibroblasts; D017988:Gammaretrovirus; D051379:Mice; D008815:Mice, Inbred Strains / Q000196:embryology; D009154:Mutation; D013539:Simian virus 40