Cell-surface mucosubstances from trypsin diaggregation of normal and virus-transformed lines of baby-hamster kidney cells.
Cell disaggregation by trypsin solubilizes significantly less mucosubstance from the surface of polyoma-virus-transformed baby-hamster kidney cells than from the same non-transformed cell line. The mucosubstance, which consists of both acid mucopolysaccharides and mucoproteins, also differs qualitatively in the two cell lines.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic; D006224:Cricetinae; D003470:Culture Media; D046508:Culture Techniques; D005688:Galactosamine / Q000302:isolation & purification; D005944:Glucosamine / Q000302:isolation & purification; D006025:Glycosaminoglycans / Q000378:metabolism*; D006595:Hexosamines / Q000302:isolation & purification; D006868:Hydrolysis; D007668:Kidney; D009088:Mucoproteins / Q000378:metabolism; D009438:Neuraminic Acids / Q000302:isolation & purification; D011120:Polyomavirus; D013462:Sulfur Radioisotopes; D014316:Tritium; D014357:Trypsin / Q000494:pharmacology*
D003470:Culture Media; D006025:Glycosaminoglycans; D006595:Hexosamines; D009088:Mucoproteins; D009438:Neuraminic Acids; D013462:Sulfur Radioisotopes; D014316:Tritium; D005688:Galactosamine; D014357:Trypsin; D005944:Glucosamine