Polyprenyl pyrophosphate-p-hydroxybenzoate polyprenyltransferase activity in mitochondria of broad-bean seeds and yeast.
Cell-free homogenates prepared from broad-bean seeds and yeast cells are capable of synthesizing 4-carboxy-2-polyprenylphenols from p-hydroxybenzoate and either isopentenyl pyrophosphate or protein-bound polyprenyl pyrophosphates (produced by incubating a Micrococcus lysodeikticus extract with isopentenyl pyrophosphate). The mitochondria contained all the polyprenyl pyrophosphate-p-hydroxybenzoate polyprenyltransferase activity; however, unlike the homogenates they could not synthesize a side chain from isopentenyl pyrophosphate and had to be provided with protein-bound polyprenyl pyrophosphates.
D001565:Benzoates / Q000378:metabolism; D002250:Carbon Radioisotopes; D002855:Chromatography, Thin Layer; D011756:Diphosphates / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology*; D012440:Saccharomyces / Q000201:enzymology*; D012639:Seeds / Q000201:enzymology*; D013729:Terpenes; D014166:Transferases / Q000378:metabolism*
D001565:Benzoates; D002250:Carbon Radioisotopes; D011756:Diphosphates; D013729:Terpenes; D014166:Transferases