Cyclic adenosine 3',5'-monophosphate in Escherichia coli.
The concentration of cyclic adenosine 3',5'-monophosphate (c-AMP) in Escherichia coli growing on different sources of carbon was studied. Cultures utilizing a source of carbon that supported growth relatively poorly had consistently higher concentrations of c-AMP than did cultures utilizing sugars that supported rapid growth. This relationship was also observed in strains defective in c-AMP phosphodiesterase and simultaneously resistant to catabolite repression; in such strains the c-AMP concentration was slightly higher for several sources of carbon tested. Cultures continued to synthesize c-AMP and secreted it into the medium, under conditions that brought about an inhibition of the intracellular accumulation of the cyclic nucleotide. Transient repression of the synthesis of beta-galactosidase was not associated with an abrupt decrease in the cellular concentration of c-AMP.
D001426:Bacterial Proteins / Q000378:metabolism; D003470:Culture Media; D000242:Cyclic AMP / Q000096:biosynthesis / Q000378:metabolism*; D004794:Enzyme Repression; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D005696:Galactosidases / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D011485:Protein Binding; D013997:Time Factors
D001426:Bacterial Proteins; D003470:Culture Media; D000242:Cyclic AMP; D010727:Phosphoric Diester Hydrolases; D005696:Galactosidases; D005947:Glucose