Initiation and direction of RNA transcription by vesicular stomatitis virus virion transcriptase.
The initiation of RNA transcription by the virion-bound RNA transcriptase of vesicular stomatitis virus has been examined. Multiple initiation sequences have been observed, two of which have been characterized (pppApCpGp... and pppGpCp...) suggestive of a transcription process which can start at different sites along the template RNA. By the use of sequential labeling techniques and exonucleases, it has been determined that there is a 5' to 3' direction of product RNA synthesis.
D001483:Base Sequence; D002845:Chromatography; D002855:Chromatography, Thin Layer; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006878:Hydroxides; D009698:Nucleoproteins / Q000378:metabolism; D009711:Nucleotides / Q000378:metabolism; D010759:Phosphorus Isotopes; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis*; D012260:Ribonucleases; D014158:Transcription, Genetic; D014316:Tritium; D014721:Vesicular stomatitis Indiana virus / Q000201:enzymology* / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism
D006878:Hydroxides; D009698:Nucleoproteins; D009711:Nucleotides; D010759:Phosphorus Isotopes; D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium; D012321:DNA-Directed RNA Polymerases; D012260:Ribonucleases