Fractionation of islet tissue in processing proinsulin to insulin.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002499:Centrifugation, Density Gradient; D006056:Golgi Apparatus / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000096:biosynthesis*; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D003012:Microbial Collagenase; D009994:Osmolar Concentration; D011384:Proinsulin / Q000378:metabolism*; D051381:Rats; D014316:Tritium
D007328:Insulin; D014316:Tritium; D011384:Proinsulin; D003012:Microbial Collagenase; D007930:Leucine