Comparison of methods for the recovery of virus inoculated into ground beef.
Various methods for the recovery of virus inoculated into ground beef were investigated in an attempt to develop a sensitive system that could be used to detect viral contaminants in market foods. A 100-g sample, inoculated with poliovirus 1, was suspended in 150 to 900 ml of Eagle minimum essential medium, pH 8.5, and mixed in either plastic bags or plastic cups on a mechanical shaker. The particulate materials were removed by means of cheese cloth, glass wool, woven fiber glass, or low-speed centrifugation. Large volumes of fluid were concentrated by ultrafiltration. Microbiological contamination was controlled by high antibiotic concentrations or by filtration. Quantitative plaque-forming-unit recovery of the virus was determined by utilizing an agar overlay technique on Vero cell cultures. The data indicated that from 20 to 50% of the seeded virus could be recovered from a 100-g sample of ground beef. The glass wool and woven fiber glass methods were the most effective, with recovery of approximately 50% of the inoculated virus.
D000085:Acetates; D000818:Animals; D000900:Anti-Bacterial Agents; D001431:Bacteriological Techniques; D002254:Carbonates; D002417:Cattle; D002482:Cellulose; D002498:Centrifugation; D003470:Culture Media; D046508:Culture Techniques; D005506:Food Contamination; D005516:Food Microbiology; D005898:Glass; D000882:Haplorhini; D007668:Kidney / Q000166:cytology; D008460:Meat; D017955:Poliovirus; D012834:Silver; D014462:Ultrafiltration; D010948:Viral Plaque Assay
D000085:Acetates; D000900:Anti-Bacterial Agents; D002254:Carbonates; D003470:Culture Media; D012834:Silver; D002482:Cellulose