Ribonucleic acid regulation in premeabilized cells of Escherichia coli capable of ribonucleic acid and protein synthesis.
A cell permeabilization procedure is described that reduces viability less than 10% and does not significantly reduce the rates of ribonucleic acid and protein synthesis when appropriately supplemented. Permeabilization abolishes the normal stringent coupling of protein and ribonucleic acid synthesis.
D001426:Bacterial Proteins / Q000096:biosynthesis*; D001431:Bacteriological Techniques; D002250:Carbon Radioisotopes; D002452:Cell Count; D003080:Cold Temperature; D000242:Cyclic AMP / Q000494:pharmacology; D004926:Escherichia coli / Q000166:cytology / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D005696:Galactosidases / Q000096:biosynthesis; D006160:Guanosine Triphosphate / Q000096:biosynthesis; D008274:Magnesium; D009693:Nucleic Acid Hybridization; D010539:Permeability; D010649:Phenylalanine / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D012329:RNA, Bacterial / Q000096:biosynthesis*; D012335:RNA, Ribosomal / Q000096:biosynthesis; D012293:Rifampin / Q000494:pharmacology; D014316:Tritium; D014325:Tromethamine; D014500:Uracil Nucleotides / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D001426:Bacterial Proteins; D002250:Carbon Radioisotopes; D010761:Phosphorus Radioisotopes; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D014500:Uracil Nucleotides; D014325:Tromethamine; D014316:Tritium; D010649:Phenylalanine; D006160:Guanosine Triphosphate; D000242:Cyclic AMP; D005696:Galactosidases; D008274:Magnesium; D012293:Rifampin; D014529:Uridine