Gel electrophoresis of avian leukosis and sarcoma viral RNA in formamide: comparison with other viral and cellular RNA species.
Class a and class b 30 to 40S RNA subunits obtained by heat dissociation from the 60 to 70S RNA complex of avian tumor viruses were compared with several RNA standards by electrophoresis in formamide-polyacrylamide gels. Class a RNA was found to have a lower electrophoretic mobility and hence probably a higher molecular weight than class b RNA. The absolute molecular weight of class a and b RNA could not be determined with accuracy, because the relationship between logarithm of molecular weight and mobility of the RNA standards was not linear. The size of class a RNA fell into the range of 2.4 x 10(6) to 3.4 x 10(6) daltons and that of class b into the range of 2.2 x 10(6) to 2.9 x 10(6) daltons, depending on the standards used. The possible biological significance of this difference is discussed.
D001355:Alpharetrovirus / Q000032:analysis*; D000818:Animals; D001358:Avian Sarcoma Viruses / Q000032:analysis; D002417:Cattle; D002460:Cell Line; D002478:Cells, Cultured / Q000032:analysis*; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts; D005559:Formamides; D007668:Kidney; D008722:Methods; D008970:Molecular Weight; D010222:Parainfluenza Virus 1, Human / Q000032:analysis*; D010761:Phosphorus Radioisotopes; D012335:RNA, Ribosomal / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D014027:Tobacco Mosaic Virus / Q000032:analysis*; D014316:Tritium; D014776:Virus Cultivation
D005559:Formamides; D010761:Phosphorus Radioisotopes; D012335:RNA, Ribosomal; D012367:RNA, Viral; D014316:Tritium