Transformation of cultured mammalian cells by viable herpes simplex virus subtypes 1 and 2.
A continuous line of rodent cells carrying the genome of the Rous sarcoma virus was stably transformed by exposure to viable herpes simplex virus of either subtype 1 or 2. Transformation was accompanied by alteration of morphology, growth characteristics, acquisition of a thymidine kinase activity (EC 2.7.1.21) resembling the corresponding specific activity of the enzyme in the herpes simplex virus, and capacity to express continuously some antigens specific for herpes simplex virus.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001358:Avian Sarcoma Viruses; D002454:Cell Differentiation; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction; D005455:Fluorescent Antibody Technique; D006358:Hot Temperature; D005779:Immunodiffusion; D011817:Rabbits / Q000276:immunology; D051381:Rats; D018139:Simplexvirus / Q000254:growth & development* / Q000276:immunology; D013937:Thymidine Kinase / Q000032:analysis / Q000378:metabolism
D000956:Antigens, Viral; D013937:Thymidine Kinase