Synthesis of infective poliovirus in BSC-1 monkey cells enucleated with cytochalasin B.
BSC-1 monkey kidney cells were enucleated by two cycles of centrifugation in the presence of cytochalasin B. The resulting populations contained 99.5 percent enucleated cells. After infection, newly synthesized poliovirus was recovered from the enucleated populations. Final virus yield per enucleated cell was about one-fifth the yield per infected untreated cell
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002458:Cell Fractionation; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects; D002478:Cells, Cultured; D003571:Cytochalasin B / Q000494:pharmacology; D003593:Cytoplasm / Q000276:immunology; D000882:Haplorhini; D007211:Indoles / Q000494:pharmacology*; D007668:Kidney; D008856:Microscopy, Fluorescence; D003904:Mitosporic Fungi; D017955:Poliovirus / Q000254:growth & development* / Q000276:immunology; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000187:drug effects*
D000956:Antigens, Viral; D007211:Indoles; D003571:Cytochalasin B