Effects of alpha-amanitin on the stimulation of prostatic ribonucleic acid polymerase by prostatic steroid-protein receptor complexes.
Stimulation of prostatic RNA polymerase in vitro by prostatic 17beta-hydroxy-5alpha-androstan-3-one (5alpha-dihydrotestosterone)-receptor complexes has been previously reported. By use of the selective inhibitor, alpha-amanitin, we have shown that both nucleolar and extranucleolar RNA polymerase activities may be stimulated, but stimulation is abolished at high ionic strength.
D000818:Animals; D001487:Basidiomycota; D002250:Carbon Radioisotopes; D002369:Castration; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D013196:Dihydrotestosterone / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D008297:Male; D009183:Mycotoxins / Q000494:pharmacology*; D009842:Oligopeptides / Q000494:pharmacology*; D010456:Peptides, Cyclic / Q000494:pharmacology; D011467:Prostate / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface; D014500:Uracil Nucleotides
D002250:Carbon Radioisotopes; D009183:Mycotoxins; D009842:Oligopeptides; D010456:Peptides, Cyclic; D011956:Receptors, Cell Surface; D014500:Uracil Nucleotides; D013196:Dihydrotestosterone; D012321:DNA-Directed RNA Polymerases