Effect of viral double-stranded RNA on protein synthesis in intact cells.
The addition of purified, noninfectious, double-stranded RNA of bovine enterovirus, a picornavirus, to intact cells in culture results in a rapid cessation of cellular polypeptide synthesis. This inhibition is specific for host cell protein synthesis since the translation of picornavirus-specific proteins is not affected by the double-stranded viral RNA.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002247:Carbon Isotopes; D002286:Carcinoma, Ehrlich Tumor; D002460:Cell Line; D002478:Cells, Cultured / Q000187:drug effects; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D004770:Enterovirus / Q000378:metabolism; D007739:L Cells; D008574:Mengovirus / Q000378:metabolism; D051379:Mice; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000302:isolation & purification / Q000494:pharmacology*; D012260:Ribonucleases; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000096:biosynthesis
D000596:Amino Acids; D002247:Carbon Isotopes; D009363:Neoplasm Proteins; D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium; D012260:Ribonucleases; D014529:Uridine