Absence of a specific ganglioside galactosyltransferase in mouse cells transformed by murine sarcoma virus.
The ganglioside composition of a nonproducer subclone (derived from BALB/c 3T3 mouseembryo cells) transformed by Kirsten murine sarcoma virus was drastically altered compared to the nontransformed parental clone. The transformed clone was unable to synthesize the mono- and disialogangliosides, G(M1) and G(D1a), due to the complete absence of a specific galactosyltransferase. The lack of this enzyme activity was established by sensitive radiochemical and enzymological techniques in vivo and in vitro.
D000818:Animals; D002250:Carbon Radioisotopes; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004622:Embryo, Mammalian; D005347:Fibroblasts; D005690:Galactose; D017988:Gammaretrovirus; D005732:Gangliosides / Q000096:biosynthesis*; D005944:Glucosamine / Q000378:metabolism; D006602:Hexosyltransferases / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C
D002250:Carbon Radioisotopes; D005732:Gangliosides; D006602:Hexosyltransferases; D005944:Glucosamine; D005690:Galactose