Correlation between the antiviral effect of interferon treatment and the inhibition of in vitro mRNA translation in noninfected L cells.
When noninfected L-cell suspension cultures are treated with interferon (specific activities superior to 10(6) reference units per mg of protein), the cell-free cytoplasmic extracts obtained are inactive for the translation of exogenous natural mRNAs. The dose-response curve shows that comparable amounts of interferon are required to produce a 50% reduction of Mengo virus multiplication in vivo and Mengo RNA translation in vitro. With higher doses of interferon, Mengo RNA translation is completely abolished, while poly U translation and endogenous protein synthesis are only slightly affected. The inactivation of Mengo RNA translation is reversible; after removal of interferon, normal translation activity is regained together with the ability to support Mengo virus multiplication. Fractionation of the cell-free extracts shows that the effect is localized in the fraction which can be washed off the ribosomes by high salt. These results establish that interferon induces a block in genetic translation in noninfected L cells.
D000818:Animals; D002250:Carbon Radioisotopes; D002474:Cell-Free System; D004305:Dose-Response Relationship, Drug; D007372:Interferons / Q000494:pharmacology*; D007739:L Cells / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D008574:Mengovirus / Q000254:growth & development* / Q000378:metabolism; D051379:Mice; D010649:Phenylalanine / Q000378:metabolism; D011072:Poly U / Q000096:biosynthesis; D014176:Protein Biosynthesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000096:biosynthesis*; D012367:RNA, Viral / Q000096:biosynthesis; D012270:Ribosomes / Q000378:metabolism; D013997:Time Factors; D014779:Virus Replication / Q000187:drug effects*
D002250:Carbon Radioisotopes; D012333:RNA, Messenger; D012367:RNA, Viral; D011072:Poly U; D010649:Phenylalanine; D007372:Interferons; D007930:Leucine