Biogenic amines in cultured neuroblastoma and astrocytoma cells.
The presence of biogenic amines in cultured cells of mouse neuroblastoma C-1300 (clone NB-2a) was suggested by fluorescence-microscope histochemistry. Incubation in media containing L-[(14)C]tyrosine and L-[(14)C]tryptophan for 24 h, followed by high-voltage electrophoresis, radiochromatogram scanning, and scintillation counting, confirmed the presence of [(14)C]dopamine, [(14)C]norepinephrine, [(14)C]epinephrine, [(14)C]serotonin, [(14)C]tyramine, and [(14)C]octopamine. Dopamine, norepinephrine, epinephrine, and serotonin were demonstrated spectrophotofluorometrically in concentrations, expressed as micrograms amine per milligram protein, of 1.19, 0.027, 0.038, and 0.148, respectively, for cells in a stationary growth phase. Fluorescence-microscope histochemistry also suggested the presence of biogenic amines in cultured astrocytoma cells (cell line C6). Spectrophotofluorometric assay of cells in a stationary growth phase demonstrated intracellular dopamine, norepinephrine, epinephrine, and serotonin in concentrations significantly lower than those of neuroblastoma cells.
D000818:Animals; D001254:Astrocytoma / Q000032:analysis*; D001679:Biogenic Amines / Q000032:analysis*; D002250:Carbon Radioisotopes; D002460:Cell Line; D002478:Cells, Cultured; D002999:Clone Cells; D004298:Dopamine / Q000032:analysis; D004590:Electrophoresis, Paper; D004837:Epinephrine / Q000032:analysis; D005557:Formaldehyde; D006651:Histocytochemistry; D051379:Mice; D008856:Microscopy, Fluorescence; D009380:Neoplasms, Nerve Tissue; D009447:Neuroblastoma / Q000032:analysis*; D009638:Norepinephrine / Q000032:analysis; D009655:Octopamine / Q000032:analysis; D051381:Rats; D012701:Serotonin / Q000032:analysis; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism; D014364:Tryptophan; D014439:Tyramine / Q000032:analysis; D014443:Tyrosine
D001679:Biogenic Amines; D002250:Carbon Radioisotopes; D009655:Octopamine; D005557:Formaldehyde; D012701:Serotonin; D014443:Tyrosine; D014364:Tryptophan; D004298:Dopamine; D009638:Norepinephrine; D014439:Tyramine; D004837:Epinephrine