Bioassay for the isolation of Dictyostelium discoideum mutants deficient in extracellular accumulation of cyclic adenosine 3',5'-monophosphate.
The growth response to external cyclic adenosine 3',5'-monophosphate of a strain of Escherichia coli deleted for adenyl cyclase was utilized to screen for mutants of Dictyostelium discoideum unable to accumulate this chemical extracellularly. The threshold amount of cyclic adenosine 3',5'-monophosphate able to induce growth of this bacterium was 3 to 4 mug/ml at 37 C and approximately 25 mug/ml at 27 C. Conditions are described that permit the detection of as low as 2 mug of this chemical at either temperature.
D000262:Adenylyl Cyclases / Q000378:metabolism; D001681:Biological Assay; D000242:Cyclic AMP / Q000378:metabolism*; D004352:Drug Resistance, Microbial; D004754:Enterobacter / Q000254:growth & development; D004926:Escherichia coli / Q000254:growth & development; D008722:Methods; D009154:Mutation; D009235:Myxomycetes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D013307:Streptomycin / Q000494:pharmacology; D013696:Temperature
D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D013307:Streptomycin