Nuclear ribonucleoprotein complexes containing polyadenylate from mouse ascites cells.
Nuclear poly(A)-containing RNA of mouse ascites cells can be extracted in the form of 15-17S ribonucleoprotein complexes under conditions in which the bulk of the heterogeneous nuclear RNA is released as 30S complexes. The poly(A)-containing fraction of nuclear extracts has been resolved into two distinct components, 15 and 17 S; neither contains the two polypeptides of 30S ribonucleoprotein. The 17S particle contains approximately six polypeptide species of molecular masses 17,000-30,000 daltons. The 15S complex has four distinct polypeptides of higher molecular mass, including a prominent 80,000-dalton species.
D000227:Adenine Nucleotides / Q000032:analysis*; D000241:Adenosine / Q000378:metabolism; D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D008113:Liver Neoplasms / Q000378:metabolism; D051379:Mice; D008851:Micropore Filters; D009374:Neoplasms, Experimental / Q000378:metabolism; D009698:Nucleoproteins / Q000032:analysis*; D010455:Peptides / Q000032:analysis; D011119:Polynucleotides / Q000032:analysis*; D012313:RNA / Q000032:analysis*; D014316:Tritium
D000227:Adenine Nucleotides; D009698:Nucleoproteins; D010455:Peptides; D011119:Polynucleotides; D014316:Tritium; D012313:RNA; D000241:Adenosine