Analysis of a viral agent isolated from multiple sclerosis brain tissue: characterization as a parainfluenzavirus type 1.
A virus originally isolated from cell cultures obtained by lysolecithin-induced fusion of human multiple sclerosis brain cells with CV-1 cells has been analyzed for its antigenic, RNA, and polypeptide compositions, and for selective biological properties. Our findings establish that this isolate, designated 6/94 virus, contains a 50S RNA genome and is, as yet, indistinguishable from Sendai virus in its antigenic and total polypeptide compositions. Despite these similarities, the 6/94 and Sendai viruses differ in certain phenotypic properties. 6/94 virus is markedly less cytocidal for chick fibroblasts, especially at 37 C and, after beta-propiolactone inactivation, it possesses a greater capacity for cell fusion and a lower toxicity than does comparably treated Sendai virus. In addition, 6/94 virus shows greater hemolytic activity.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001921:Brain / Q000382:microbiology* / Q000473:pathology; D002459:Cell Fusion; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D000882:Haplorhini; D006461:Hemolysis; D006801:Humans; D006822:Hybrid Cells / Q000382:microbiology; D007668:Kidney; D009103:Multiple Sclerosis / Q000382:microbiology*; D010222:Parainfluenza Virus 1, Human / Q000032:analysis; D010455:Peptides / Q000032:analysis; D018108:Respirovirus / Q000032:analysis / Q000302:isolation & purification*; D014776:Virus Cultivation
D000956:Antigens, Viral; D010455:Peptides