title: Biochemical studies on the chi mutation of bacteriophage T4: differential inhibition of chi+ and chi DNA synthesis by mitomycin C.
abstract: Biochemical studies were carried out to determine the effect of chi mutation on T4 DNA synthesis. The rate and final extent of DNA synthesis are almost the same with T4D- and T4chi-infected cells, although the burst size of T4chi is about one-sixth that of the wild type. The DNA synthesis of T4chi-infected cells is more readily inhibited by mitomycin C than is that of T4 wild type. When mitomycin C was added during active phage growth, DNA synthesis of T4chi halted almost immediately. T4 DNA polymerases isolated from chi(+)- and chi-infected cells, however, exhibit no difference with regard to their sensitivities to mitomycin C, priming activities with alkylated or ultraviolet light-irradiated templates and other enzymatic properties.
mesh_terms: D000476:Alkanesulfonates / Q000494:pharmacology; D000478:Alkylation; D000818:Animals; D002250:Carbon Radioisotopes; D003090:Coliphages / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D004247:DNA / Q000378:metabolism / Q000528:radiation effects; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis*; D004926:Escherichia coli; D006358:Hot Temperature; D008297:Male; D008937:Mitomycins / Q000494:pharmacology*; D009154:Mutation; D010761:Phosphorus Radioisotopes; D011830:Radiation Effects; D012474:Salmon; D013094:Spermatozoa; D013698:Templates, Genetic; D013941:Thymine / Q000378:metabolism; D013942:Thymine Nucleotides / Q000378:metabolism; D014316:Tritium; D014466:Ultraviolet Rays
chemical_list: D000476:Alkanesulfonates; D002250:Carbon Radioisotopes; D004279:DNA, Viral; D008937:Mitomycins; D010761:Phosphorus Radioisotopes; D013942:Thymine Nucleotides; D014316:Tritium; D004247:DNA; D004254:DNA Nucleotidyltransferases; D013941:Thymine