title: Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. V. Isolation of additional hybrids which differ in their simian virus 40-specific biological properties.
abstract: Four new nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid viruses have been isolated. Although these viruses (designated Ad2(+)ND(2), Ad2(+)ND(3), Ad2(+)ND(4), and Ad2(+)ND(5)) were clonal derivatives of the same Ad2-SV40 hybrid population, they differ significantly from each other and from the previously isolated nondefective hybrid, Ad2(+)ND(1), in their biological properties or in the amount of SV40-specific RNA induced during lytic infection.Like Ad2(+)ND(1), Ad2(+)ND(2), and Ad2(+)ND(4) pass serially in both human embryonic kidney (HEK) and primary African green monkey kidney cells. In contrast, Ad2(+)ND(3) and Ad2(+)ND(5) pass serially only in HEK cells. Ad2(+)ND(2) is like Ad2(+)ND(1) in that it induces the SV40 U antigen, but not SV40 T antigen; however, in contrast to the perinuclear SV40 antigen induced by Ad2(+)ND(1), the SV40 antigen induced by Ad2(+)ND(2) is located peripherally in the cytoplasm as well as in the perinuclear region of infected cells. Ad2(+)ND(4) induces both the SV40 T and U antigens. Ad2(+)ND(3) and Ad2(+)ND(5) do not induce serologically detectable SV40 antigens and are distinguished from each other on the basis of the relative quantities of SV40-specific RNA which they induce. The induction of different SV40-specific functions suggests the incorporation of different segments of SV40 DNA within the genomes of the respective hybrid viruses.
mesh_terms: D000256:Adenoviridae / Q000032:analysis / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification*; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D046508:Culture Techniques; D004279:DNA, Viral / Q000032:analysis; D000882:Haplorhini; D006649:Histocompatibility Antigens / Q000032:analysis; D006801:Humans; D006824:Hybridization, Genetic; D007668:Kidney; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis; D013539:Simian virus 40 / Q000032:analysis / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification*; D014316:Tritium; D010948:Viral Plaque Assay; D014776:Virus Cultivation; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral; D006649:Histocompatibility Antigens; D012367:RNA, Viral; D014316:Tritium