Glycosyltransferases in the Golgi membranes of onion stem.
Cell fractions consisting largely of Golgi membranes were prepared from the meristematic region of the onion. Several enzyme activities were found to be localized in these fractions: inosine diphosphatase, galactosyltransferases and glucosyltransferases. The fractions catalysed the transfer of [(14)C]galactose from UDP-galactose to endogenous and cell-sap acceptors, to N-acetylglucosamine and to ovalbumin. In the presence of bovine alpha-lactalbumin, transfer to glucose (lactose synthesis) was catalysed. [(14)C]Glucose was transferred from UDP-glucose to endogenous and cell-sap acceptors, to cellobiose and to fructose (sucrose synthesis). All these activities were latent, being potentiated by detergents (Triton X-100 or sodium deoxycholate). The characteristics of some of these enzyme activities are described and their biological significance is discussed.
D002250:Carbon Radioisotopes; D002499:Centrifugation, Density Gradient; D003902:Detergents / Q000378:metabolism; D005690:Galactose; D005944:Glucosamine / Q000378:metabolism; D005964:Glucosyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D006056:Golgi Apparatus / Q000201:enzymology*; D006602:Hexosyltransferases; D007288:Inosine; D007700:Kinetics; D008566:Membranes / Q000201:enzymology; D008854:Microscopy, Electron; D010047:Ovalbumin / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000302:isolation & purification; D010944:Plants / Q000201:enzymology*; D013347:Subcellular Fractions; D014500:Uracil Nucleotides / Q000378:metabolism
D002250:Carbon Radioisotopes; D003902:Detergents; D014500:Uracil Nucleotides; D007288:Inosine; D010047:Ovalbumin; D005964:Glucosyltransferases; D006602:Hexosyltransferases; D010744:Phosphoric Monoester Hydrolases; D005944:Glucosamine; D005690:Galactose