A paracrystalline inclusion formed during sporulation of enterotoxin-producing strains of Clostridium perfringens type A.
A large paracrystalline inclusion is formed by certain strains of Clostridium perfringens type A during spore morphogenesis. In most cell thin sections, the inclusion appeared rod-shaped when sectioned at an angle perpendicular to its longer axis, and circular or oval-shaped when sectioned at an angle parallel to its longer axis. Measurements performed on electron micrographs of inclusions sectioned to reveal the rod shape indicated a fairly consistent thickness (width) of 192 +/- 23 nm. The length of the inclusions varied considerably with a maximum of approximately 2,120 nm being observed. Ultrastructurally, the inclusion was composed of closely packed, periodically spaced, parallel layers. Usually a single inclusion was randomly located in the cytoplasm of the cell. Two inclusions per cell were rarely observed. The inclusion was formed only by ent(+) strains of C. perfringens. Mutants of the ent(+) strain NCTC 8798 that were altered in their sporulating and enterotoxin-producing capacities and revertants of these mutants were tested for inclusion formation. The results indicate that, as with the ent(+) trait, a direct relationship exists between inclusion formation and spore formation. The synthesis of enterotoxin, formation of a morphologically distinct inclusion, and the initial deposition of discontinuous coat fragments around the forespore appear to be events closely related in time during spore morphogenesis.
D003013:Clostridium / Q000254:growth & development; D003016:Clostridium perfringens / Q000166:cytology* / Q000378:metabolism; D003461:Crystallography; D004768:Enterotoxins / Q000096:biosynthesis*; D005796:Genes; D002479:Inclusion Bodies; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D009154:Mutation; D012703:Serotyping; D013170:Spores / Q000254:growth & development*; D013171:Spores, Bacterial / Q000166:cytology / Q000254:growth & development
D004768:Enterotoxins