Separation of the two non-identical subunits of lombricine kinase from Lumbricus terrestris muscle by chromatography on sepharose-mercurial. Isolation of the tryptic peptide containing its essential thiol group.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001555:Benzene Derivatives; D001665:Binding Sites; D002250:Carbon Radioisotopes; D002268:Carboxypeptidases; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D005033:Ethylmaleimide; D006146:Guanidines; D007461:Iodoacetates; D008970:Molecular Weight; D009132:Muscles / Q000201:enzymology*; D009835:Oligochaeta / Q000201:enzymology*; D010446:Peptide Fragments / Q000302:isolation & purification; D010770:Phosphotransferases / Q000302:isolation & purification; D017852:Phosphotransferases (Nitrogenous Group Acceptor); D011134:Polysaccharides; D011485:Protein Binding; D011487:Protein Conformation; D012694:Serine; D013438:Sulfhydryl Compounds; D014357:Trypsin; D014508:Urea
D000596:Amino Acids; D001555:Benzene Derivatives; D002250:Carbon Radioisotopes; D006146:Guanidines; D007461:Iodoacetates; D010446:Peptide Fragments; D011134:Polysaccharides; D013438:Sulfhydryl Compounds; D012694:Serine; D014508:Urea; D010770:Phosphotransferases; D017852:Phosphotransferases (Nitrogenous Group Acceptor); C021517:lombricine kinase; D002268:Carboxypeptidases; D014357:Trypsin; D005033:Ethylmaleimide