title: Development of the activities of enzymes of the isoprenoid pathway during early stages of pea-seed germination.
abstract: The activities of individual enzymes of the isoprenoid pathway from mevalonate kinase to squalene synthetase in homogenates of seeds germinated up to 32h were assayed. Changes in the activity of each enzyme were observed and compared with the activity at the 2h germination stage. Activities of alkaline phosphatase and fructose 1,6-diphosphate aldolase were similarly measured to provide a reference for changes in the general metabolic activity of seeds during imbibition of water. Water uptake reached a plateau after 12h. The reference enzymes almost doubled in activity between 2 and 8h and thereafter their activities steadily declined. All of the enzymes of the isoprenoid pathway increased in activity between 2 and 6h and, thereafter, with the exception of the prenyltransferase, their activities remained relatively constant. With the prenyltransferase activity the initial increase was followed by a short plateau between 6 and 9h and then a second increase to a maximum between 14 and 16h. After 16h the activity declined. The relative activities of the isoprenoid enzymes at 16h of germination were mevalonate kinase>phosphomevalonate kinase>pyrophosphomevalonate decarboxylase approximately isopentenyl pyrophosphate isomerase>squalene synthetase>isopentenyl pyrophosphate/dimethylallyl pyrophosphate prenyltransferase. The finding that the prenyltransferase may be the rate-limiting enzyme in squalene synthesis from mevalonate is discussed in relation to regulation of isoprenoid synthesis during pea-seed germination.
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000475:Alkenes; D002245:Carbon Dioxide / Q000378:metabolism; D002262:Carboxy-Lyases / Q000378:metabolism; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D007535:Isomerases / Q000378:metabolism; D007700:Kinetics; D008025:Ligases / Q000378:metabolism; D008798:Mevalonic Acid; D010756:Phosphoric Acids; D010770:Phosphotransferases / Q000378:metabolism*; D012639:Seeds / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D013185:Squalene; D013729:Terpenes / Q000378:metabolism*; D013997:Time Factors; D014166:Transferases / Q000378:metabolism; D014867:Water / Q000378:metabolism
chemical_list: D000475:Alkenes; D010756:Phosphoric Acids; D013729:Terpenes; D014867:Water; D002245:Carbon Dioxide; D013185:Squalene; D014166:Transferases; D010770:Phosphotransferases; D000469:Alkaline Phosphatase; D002262:Carboxy-Lyases; D005634:Fructose-Bisphosphate Aldolase; D007535:Isomerases; D008025:Ligases; D008798:Mevalonic Acid