Growth characteristics of cytomegalovirus in human fibroblasts with demonstration of protein synthesis early in viral replication.
In high-multiplicity infection of human fibroblasts, human cytomegalovirus of WI-38 human diploid cells produced early cell rounding 6 to 24 h after inoculation. This early cell rounding was caused only by inoculation with infectious virions. Inhibitors of protein synthesis, but not DNA inhibitors, prevented this cytopathic effect. Apparently, a new protein is synthesized in infected fibroblasts from about 2 h postinoculation. Infectivity of cell-associated and supernatant infectious virus reached maximal levels at 5 to 7 and 10 days postinoculation, respectively. Synthesis of DNA, infectious virus, complement-fixing antigen, and precipitin antigen all began between 24 and 48 h, with the bulk of synthesis occurring 48 to 96 h postinoculation.
D000596:Amino Acids / Q000378:metabolism; D000956:Antigens, Viral / Q000032:analysis; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002247:Carbon Isotopes; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D003168:Complement Fixation Tests; D003561:Cytarabine / Q000494:pharmacology; D003587:Cytomegalovirus / Q000254:growth & development* / Q000276:immunology / Q000378:metabolism; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000096:biosynthesis; D005467:Floxuridine / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D007181:Inclusion Bodies, Viral; D008168:Lung; D011233:Precipitin Tests; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium; D014764:Viral Proteins / Q000096:biosynthesis*; D014779:Virus Replication
D000596:Amino Acids; D000956:Antigens, Viral; D002247:Carbon Isotopes; D004279:DNA, Viral; D014764:Viral Proteins; D005467:Floxuridine; D003561:Cytarabine; D014316:Tritium; D001973:Bromodeoxyuridine; D013936:Thymidine