Separation of B and C type virions by centrifugation in gentle density gradients.
Murine type B particles were separated from type C (Rauscher leukemia virus) by means of gentle (low-increment rate) density gradients. The best separation was obtained when the density ranged from 1.13 to 1.20 g/cm(3) when sucrose was used and from 1.12 to 1.28 g/cm(3) with CsCl. The buoyant densities of the B and C particle bands in sucrose were 1.18 and 1.16 g/cm(3), respectively. The CsCl gradient gave a better separation with the B particles banding at a density of 1.20 g/cm(3) and with the C particle density little different from its value in sucrose.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D017988:Gammaretrovirus / Q000302:isolation & purification*; D008324:Mammary Tumor Virus, Mouse / Q000302:isolation & purification; D008892:Milk / Q000382:microbiology; D011925:Rauscher Virus / Q000302:isolation & purification; D012190:Retroviridae / Q000302:isolation & purification*; D013395:Sucrose
D002712:Chlorides; D002586:Cesium; D013395:Sucrose