Effect of D-penicillamine on poliovirus replication in HeLa cells.
A series of mercaptan compounds were studied with respect to their ability to inhibit the growth of poliovirus in cultured cells. Of the compounds tested only d-penicillamine possessed antiviral activity. There was no direct effect on the virus itself, nor were the processes of adsorption, penetration and uncoating, or virus-induced "shut-off" of host cell protein synthesis inhibited. At concentrations where there was no effect on host cell RNA or protein synthesis, d-penicillamine caused a marked inhibition of virus-specific RNA and protein synthesis. Although much reduced, the relative concentrations of single-stranded and double-stranded viral RNA synthesized in the presence of d-penicillamine was unchanged. Similarly, all apparent precursor and cleavage product proteins could be synthesized in the presence of the drug. The inhibitory effect was reversible, after a lag of 1.5 to 2 h after removal of the drug, and normal yields of virus could be obtained. The structural and functional properties of d-penicillamine are discussed in relation to requirements for anti-polioviral activity.
D000998:Antiviral Agents / Q000494:pharmacology; D002250:Carbon Radioisotopes; D003545:Cysteine / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D010396:Penicillamine / Q000494:pharmacology*; D017955:Poliovirus / Q000187:drug effects* / Q000254:growth & development; D012367:RNA, Viral / Q000096:biosynthesis; D013438:Sulfhydryl Compounds / Q000494:pharmacology; D013997:Time Factors; D014529:Uridine; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication / Q000187:drug effects*
D000998:Antiviral Agents; D002250:Carbon Radioisotopes; D012367:RNA, Viral; D013438:Sulfhydryl Compounds; D014764:Viral Proteins; D010396:Penicillamine; D003545:Cysteine; D014529:Uridine