title: Molecular weight determination of Sendai RNA by dimethyl sulfoxide gradient sedimentation.
abstract: The molecular weight of the large RNA of Sendai virus has been determined by sedimentation analysis in sucrose gradients containing 99% dimethyl sulfoxide (DMSO) to be 2.3 x 10(6). Sendai RNA recovered from 99% DMSO was found to cosediment with nondenatured Sendai RNA at 46 to 48s in ordinary sucrose gradients. The molecular weight value of 2.3 x 10(6) is considerably smaller than the estimates of 6 x 10(6) to 7 x 10(6) determined under nondenaturing conditions, suggesting a unique structure for Sendai RNA.
mesh_terms: D000818:Animals; D002247:Carbon Isotopes; D002478:Cells, Cultured / Q000032:analysis; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D004121:Dimethyl Sulfoxide; D007668:Kidney; D008722:Methods; D051379:Mice; D008970:Molecular Weight; D009522:Newcastle disease virus / Q000032:analysis / Q000254:growth & development; D009691:Nucleic Acid Denaturation; D010222:Parainfluenza Virus 1, Human / Q000032:analysis* / Q000254:growth & development; D010759:Phosphorus Isotopes; D012313:RNA / Q000032:analysis; D012335:RNA, Ribosomal / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D013395:Sucrose; D014316:Tritium; D014529:Uridine; D014776:Virus Cultivation
chemical_list: D002247:Carbon Isotopes; D010759:Phosphorus Isotopes; D012335:RNA, Ribosomal; D012367:RNA, Viral; D014316:Tritium; D013395:Sucrose; D012313:RNA; D014529:Uridine; D004121:Dimethyl Sulfoxide