Identification of the toxic principle in a sample of poaefusarin.
A sample of poaefusarin (a mycotoxin suspected of being one of the toxins involved in alimentary toxic aleukia in the U.S.S.R.) was received from a Soviet scientist for evaluation and comparison with other mycotoxins. Although poaefusarin is presumed to be a steroid, analyses by thin-layer chromatography, gas-liquid chromatography, and infrared, ultraviolet, and mass spectrometry could not confirm the presence of a steroid structure. However, 2.5% of the sample was made up of the trichothecene T-2 toxin, an amount sufficient to explain the toxicity found in the rat and rabbit skin toxicity tests. In addition, neosolaniol (0.14%), T-2 tetraol (0.6%), and zearalenone (F-2) (0.43%) were present in the sample. Since the toxicity was found to be associated only with T-2 toxin, no attempt was made to determine the nature of the other nontoxic components of the sample.
D000818:Animals; D002018:Bufanolides / Q000032:analysis* / Q000302:isolation & purification; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D004487:Edema / Q000139:chemically induced; D005260:Female; D005670:Fusarium; D006027:Glycosides / Q000032:analysis / Q000302:isolation & purification; D006470:Hemorrhage / Q000209:etiology; D013058:Mass Spectrometry; D009183:Mycotoxins / Q000032:analysis* / Q000302:isolation & purification / Q000633:toxicity; D011817:Rabbits; D051381:Rats; D012822:Silicon Dioxide / Q000032:analysis; D012882:Skin Tests; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
D002018:Bufanolides; D006027:Glycosides; D009183:Mycotoxins; C008942:poaefusarin; D012822:Silicon Dioxide