Rapid method to determine labeling specificity of radioactive enteroviruses.
The specificity of labeling enteroviruses with (32)P-labeled NaH(2)PO(4) or (14)C-leucine can be determined by comparing the percent adsorption of infective particles and radioactivity to membrane (Millipore) filters.
D000327:Adsorption; D000818:Animals; D002247:Carbon Isotopes; D046508:Culture Techniques; D004770:Enterovirus / Q000302:isolation & purification*; D029822:Enterovirus B, Human / Q000302:isolation & purification*; D000882:Haplorhini; D007553:Isotope Labeling; D007668:Kidney; D007930:Leucine; D008251:Macaca; D008722:Methods; D008851:Micropore Filters; D010710:Phosphates; D010759:Phosphorus Isotopes; D017955:Poliovirus / Q000302:isolation & purification*; D014776:Virus Cultivation
D002247:Carbon Isotopes; D010710:Phosphates; D010759:Phosphorus Isotopes; D007930:Leucine