Effect of insulin on the growth pattern and adenylate cyclase activity of BHK fibroblasts.
BHK 21/13 fibroblasts grown in the presence of insulin show some characteristics of a transformed strain. Cells broaden, cross each other intricately, and form colonies on both polystyrene dishes and soft agar. The effect of insulin is prevented by addition of N(6)-butyryl adenosine 3':5'-cyclic monophosphate and theophylline to the culture medium. Insulin causes a decrease in the concentration of intracellular adenosine 3':5'-cyclic monophosphate and inhibition of adenylate cyclase activity in BHK fibroblast membranes. This inhibition is observed at insulin concentrations of 0.1 nM or higher.
D000067956:Adenylyl Cyclase Inhibitors; D000262:Adenylyl Cyclases / Q000032:analysis; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D002478:Cells, Cultured; D006224:Cricetinae; D003470:Culture Media; D000242:Cyclic AMP / Q000032:analysis / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000201:enzymology; D007328:Insulin / Q000494:pharmacology*; D007329:Insulin Antagonists; D007668:Kidney; D008938:Mitosis / Q000187:drug effects*; D013806:Theophylline / Q000494:pharmacology
D000067956:Adenylyl Cyclase Inhibitors; D003470:Culture Media; D007328:Insulin; D007329:Insulin Antagonists; D013806:Theophylline; D000242:Cyclic AMP; D000262:Adenylyl Cyclases