Inactivation of herpes simplex virus by concanavalin A.
The infectivity of herpes simplex virus type 1 (HSV-1) was inactivated after treatment with either concanavalin A (ConA) or periodate. Phytohemagglutinin, wheat germ agglutinin, pokeweed mitogen, and neuraminidase failed to inactivate the virus. The effect of ConA could be specifically inhibited or reversed by the addition of alpha-methyl-d-glucoside or alpha-methyl-d-mannoside. Evidence was obtained that HSV-1 inactivated by ConA could adsorb to host cells. Viral aggregation was not a major mechanism in the inactivation of HSV-1 by ConA. Under the experimental conditions employed, inactivation of HSV-1 was faster by ConA than by antiserum and less temperature dependent. A ConA-resistant fraction was detected which appeared to adsorb less quickly than untreated virus, and penetration of ConA-resistant fraction was strikingly slow. The presence of aggregates in the virus preparation did not appear to account for the ConA-resistant fraction. Inactivation of viral infectivity by ConA was obtained only with enveloped viruses, since HSV-1, HSV-2, pseudorabies, and vesicular stomatitis virus were inactivated and vaccinia and echovirus type 6 were not.
D000256:Adenoviridae / Q000187:drug effects; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D003208:Concanavalin A / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D029822:Enterovirus B, Human / Q000187:drug effects; D005944:Glucosamine / Q000494:pharmacology; D000882:Haplorhini; D006564:Herpesviridae / Q000187:drug effects; D037102:Lectins / Q000494:pharmacology; D008251:Macaca; D008759:Methylglycosides / Q000494:pharmacology; D008934:Mitogens / Q000494:pharmacology; D010504:Periodic Acid / Q000494:pharmacology; D011557:Pseudorabies / Q000382:microbiology; D018139:Simplexvirus / Q000187:drug effects*; D014616:Vaccinia virus / Q000187:drug effects; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D037102:Lectins; D008759:Methylglycosides; D008934:Mitogens; D010504:Periodic Acid; D003208:Concanavalin A; D005944:Glucosamine