title: Separation of cells having histochemically demonstrable glucose-6-phosphatase from suspensions of hamster kidney cells in an isokinetic density gradient of Ficoll in tissue culture medium.
abstract: Suspensions of enzymatically disaggregated hamster kidney cells were separated primarily according to differences in diameter using velocity sedimentation in a previously described isokinetic density gradient and according to differences in density in an isopycnic density gradient. Cells which contained histochemically demonstrable glucose-6-phosphatase were thought to be cells from proximal tubules and constituted 46.5 +/- 14.1% of the cells in the starting sample suspension of disaggregated kidney cells. The purest gradient fractions from experiments using velocity sedimentation contained 98.0 +/- 0.6% cells which demonstrated glucose-6-phosphatase activity. More than 99.0% of these cells excluded trypan blue. Isopycnic sedimentation was not an effective means of purifying proximal tubule cells.
mesh_terms: D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D003470:Culture Media; D005952:Glucose-6-Phosphatase / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000166:cytology* / Q000201:enzymology; D008297:Male; D008722:Methods; D013395:Sucrose
chemical_list: D003470:Culture Media; D013395:Sucrose; D005952:Glucose-6-Phosphatase