Initiation of oncogenic transformation of mouse lymphocytes in vitro by Abelson leukemia virus.
While C-type RNA viruses are known to induce leukemias and lymphomas, oncogenic transformation of lymphoid cells by them in vitro has not been reported. In this study, splenocytes from female BALB/c mice were infected in vitro with Abelson virus, a C-type RNA virus that induces nonthymic lymphomas and plasmacytomas in mice. The cells were transplanted into recipients of different karyotype, either male BALB/c mice or hybrid BALB/c x AL/N (CALF1) mice, which bear the Rb(5.19)1Wh translocation. Transplants of eight of the resulting tumors (one plasmacytoma and seven lymphomas) contained cells of donor BALB/c karyotype, indicating that transformation of splenocytes occurred in vitro.
D000818:Animals; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D005260:Female; D005838:Genotype; D007621:Karyotyping; D009052:Leukemia Virus, Murine / Q000254:growth & development; D008214:Lymphocytes; D008223:Lymphoma / Q000209:etiology / Q000235:genetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000209:etiology; D010954:Plasmacytoma / Q000209:etiology / Q000235:genetics; D012190:Retroviridae / Q000254:growth & development; D013154:Spleen / Q000166:cytology