Effect of beta-propiolactone inactivation of polyoma virus on viral functions.
Polyoma virus was inactivated by treatment with beta-propiolactone. T-antigen production, polyoma-RNA synthesis, induction of host DNA synthesis (measured by incorporation of labeled thymidine into the cell culture), and in vitro transforming ability were inactivated to a similar degree by various beta-propiolactone concentrations (0.25% beta-propiolactone reduced these functions approximately 96%), whereas plaque-forming ability and the ability of the virus to replicate its DNA and to synthesize capsid antigen were inactivated by a given concentration of beta-propiolactone to a much greater degree (0.25% beta-propiolactone led to a reduction of plaque-forming ability of over 8 logs). The significance of these data and their relationship to previously published experiments are discussed.
D000818:Animals; D000956:Antigens, Viral; D001345:Autoradiography; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D046508:Culture Techniques; D004279:DNA, Viral / Q000096:biosynthesis; D006571:Heterocyclic Compounds; D006649:Histocompatibility Antigens; D007668:Kidney; D007783:Lactones / Q000494:pharmacology*; D051379:Mice; D011120:Polyomavirus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D011422:Propionates; D012367:RNA, Viral / Q000096:biosynthesis; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis
D000956:Antigens, Viral; D004279:DNA, Viral; D006571:Heterocyclic Compounds; D006649:Histocompatibility Antigens; D007783:Lactones; D011422:Propionates; D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium; D013936:Thymidine; D014529:Uridine