Characterization of RNA from noninfectious virions produced by sarcoma positive-leukemia negative transformed 3T3 cells.
RNA from noninfectious virions produced by two established clonal lines of sarcoma positive-leukemia negative (S+L-)-transformed 3T3 cells has been characterized. RNA from virions or nucleoids of S+L--(C243) cells consisted of three to four sizes: +/-44 S (6%), 28 S (17%), 18 S (38%), and <18 S (39%). 28S virion RNA contained some virus-specific information demonstrable by RNA.DNA hybridization with a DNA probe derived from the RNA-directed DNA polymerase product of murine sarcoma-leukemia virus, while parallel studies indicated that 28S ribosomal RNA from ribosomal subunits of transformed and nontransformed 3T3 cells did not contain virus-specific information. In contrast to the S+L-(C243) virions, RNA from virions or nucleoids of S+L-(D56) cells consisted of five sizes: 80 S (6%), 68 S (8%), 56 S (5%), 28 S (28%), and <28 S (53%). Thermal dissociation studies suggested that this S+L- genome is comprised of 28S RNA subunits. From these studies we postulate that the 28S viral RNA is most probably the monomeric genome of S+L- virions.
D000241:Adenosine / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002499:Centrifugation, Density Gradient; D002999:Clone Cells; D004279:DNA, Viral / Q000378:metabolism; D005347:Fibroblasts; D017988:Gammaretrovirus / Q000032:analysis*; D006378:Helper Viruses / Q000032:analysis; D051379:Mice; D008970:Molecular Weight; D008979:Moloney murine leukemia virus / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D010761:Phosphorus Radioisotopes; D011119:Polynucleotides / Q000032:analysis; D011132:Polyribosomes / Q000032:analysis; D012335:RNA, Ribosomal / Q000032:analysis; D012367:RNA, Viral / Q000096:biosynthesis / Q000302:isolation & purification* / Q000378:metabolism; D012270:Ribosomes / Q000032:analysis; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D004279:DNA, Viral; D010761:Phosphorus Radioisotopes; D011119:Polynucleotides; D012335:RNA, Ribosomal; D012367:RNA, Viral; D014316:Tritium; D000241:Adenosine; D014529:Uridine