title: The synthesis in vitro of RNA polymerase subunits of Escherichia coli.
abstract: DNA from transducing bacteriophage that carry the structural gene for the beta subunit of RNA polymerase has been used to direct protein synthesis in a cell-free transcription-translation system. A polypeptide species has been detected which appears to be beta-subunit material made in vitro.
mesh_terms: D002474:Cell-Free System; D003090:Coliphages / Q000032:analysis; D000242:Cyclic AMP / Q000494:pharmacology; D004279:DNA, Viral / Q000302:isolation & purification / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000032:analysis / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D008970:Molecular Weight; D014176:Protein Biosynthesis; D013698:Templates, Genetic; D014158:Transcription, Genetic; D014316:Tritium
chemical_list: D004279:DNA, Viral; D014316:Tritium; D000242:Cyclic AMP; D012321:DNA-Directed RNA Polymerases