Use of streptomycin and cyclic adenosine 5'-monophosphate in the isolation of mutants deficient in CAP protein.
A method is described for selective isolation of mutants deficient in CAP protein necessary for the expression of catabolite-sensitive operons in Escherichia coli.
D001089:Arabinose / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis*; D000242:Cyclic AMP / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification* / Q000378:metabolism; D005696:Galactosidases / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D007785:Lactose / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D009153:Mutagens; D009154:Mutation; D009604:Nitrosoguanidines; D009693:Nucleic Acid Hybridization; D009876:Operon; D012329:RNA, Bacterial / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D012210:Rhamnose / Q000378:metabolism; D013307:Streptomycin / Q000494:pharmacology*; D014316:Tritium; D014368:Tryptophanase / Q000096:biosynthesis; D014529:Uridine / Q000378:metabolism
D001426:Bacterial Proteins; D009153:Mutagens; D009604:Nitrosoguanidines; D012329:RNA, Bacterial; D012333:RNA, Messenger; D014316:Tritium; D001089:Arabinose; D000242:Cyclic AMP; D005696:Galactosidases; D014368:Tryptophanase; D007930:Leucine; D005947:Glucose; D007785:Lactose; D005990:Glycerol; D012210:Rhamnose; D014529:Uridine; D013307:Streptomycin