Virion-bound protein kinase in Semliki forest and Sindbis viruses.
Semliki forest virus and Sindbis virus (Alphaviruses belonging to the togavirus group) grown in BHK-21 cells possessed very low levels of virion-associated protein kinase activity. For comparison, vesicular stomatitis virus, also grown in BHK-21 cells, contained a virion-bound protein kinase which had a specific activity 80 times greater than that of the Alphaviruses. The Alphavirus protein kinase was unmasked by the nonionic detergent Nonidet P-40 but was not activated by cyclic nucleotides. Phosvitin was the best exogenous phosphate acceptor for assaying the viral enzyme in vitro. Phosphoprotein phosphatase activity was also detected in the Alphaviruses. Both in vivo and in vitro, all of the viral structural polypeptides were phosphorylated, and the phosphorylated amino acids were found to be serine and threonine. The viral nucleocapsid protein was about four times more efficient as a phosphate acceptor than were the envelope proteins. From 33 to 50% of the total protein kinase was bound to the viral nucleocapsid, and the specific activity of this enzyme was 4 to 10 times greater than that associated with the viral envelope.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D006389:Hemagglutinins, Viral; D007668:Kidney; D007930:Leucine; D009712:Nucleotides, Cyclic / Q000494:pharmacology; D010710:Phosphates; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D011485:Protein Binding; D011494:Protein Kinases / Q000378:metabolism*; D012672:Semliki forest virus / Q000201:enzymology*; D012845:Sindbis Virus / Q000201:enzymology*; D013501:Surface-Active Agents; D014316:Tritium; D014721:Vesicular stomatitis Indiana virus / Q000201:enzymology; D014764:Viral Proteins
D006389:Hemagglutinins, Viral; D009712:Nucleotides, Cyclic; D010710:Phosphates; D010761:Phosphorus Radioisotopes; D013501:Surface-Active Agents; D014764:Viral Proteins; D014316:Tritium; D011494:Protein Kinases; D010744:Phosphoric Monoester Hydrolases; D007930:Leucine