A micrometabolic inhibition test for the estimation of poliovirus neutralizing antibodies.
A modified micrometabolic inhibition test for the titration of poliovirus neutralizing antibodies is described. The effects of varying both the duration and the temperature of incubation of the serum-virus mixtures were examined. Overnight incubation resulted in a 2-3-fold increase in the titre of the hyperimmune sera tested. The method is simple and economical and appears to be suitable for antibody surveys.
D000914:Antibodies, Viral / Q000032:analysis*; D002675:Child, Preschool; D006801:Humans; D008722:Methods; D009500:Neutralization Tests; D017955:Poliovirus / Q000276:immunology*; D013696:Temperature; D013997:Time Factors
D000914:Antibodies, Viral