Capsular polysaccharide of Clostridium perfringens Hobbs 10.
A capsular polysaccharide was isolated from a strain of Clostridium perfringens Hobbs 10 type A by cold-water extraction of whole, heavily encapsulated cells. The water-soluble polymer was isolated by alcohol precipitation and purified by treatment with chloroform-butanol, cetytrimethylammonium bromide, and column gel permeation chromatography by using Bio-Gel A-5m agarose. The formation of a single precipitin line, when the isolated polysaccharide was reacted with its homologous antisera by double diffusion in gel, was considered a criterion of immunochemical purity. The purified polymer appeared as a single peak when eluted from diethylaminoethyl-Sephadex with a linear gradient of NaCl. The polysaccharide was composed of glucose, galactose, galactosamine, and iduronic acid in a molar ratio of 4.1:5.1.7:1, respectively. These constituents accounted for 83% of the dry weight. The polysaccharide appeared to have a molecular weight of 40,000 and exhibited aggregation up to 120,000. A trace of peptide material could not be removed during purification.
D011232:Chemical Precipitation; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002854:Chromatography, Paper; D003016:Clostridium perfringens / Q000032:analysis*; D005688:Galactosamine / Q000032:analysis; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006595:Hexosamines / Q000032:analysis; D005779:Immunodiffusion; D008970:Molecular Weight; D010710:Phosphates / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D011506:Proteins / Q000032:analysis; D014574:Uronic Acids / Q000032:analysis
D006595:Hexosamines; D010710:Phosphates; D011135:Polysaccharides, Bacterial; D011506:Proteins; D014574:Uronic Acids; D005688:Galactosamine; D005947:Glucose; D005690:Galactose