Two-hour embedding procedure for intracellular detection of viruses by electron microscopy.
An embedding method requiring only 2 h to complete and giving excellent ultrastructural preservation has been used for the rapid detection of viruses in tissue cultures. The method has also been applied successfully to mammalian tissue. It provides a rapid technique for identifying viruses isolated in tissue cultures, for screening cultures for adventitious agents, and for examining tissue biopsies for viruses.
D000256:Adenoviridae / Q000302:isolation & purification; D000818:Animals; D002460:Cell Line; D046508:Culture Techniques; D005976:Glutaral; D000882:Haplorhini; D006652:Histological Techniques; D007668:Kidney; D008099:Liver / Q000166:cytology; D008722:Methods; D051379:Mice; D008854:Microscopy, Electron; D009992:Osmium; D010087:Oxides; D018139:Simplexvirus / Q000302:isolation & purification; D013997:Time Factors; D014780:Viruses / Q000302:isolation & purification*
D010087:Oxides; D009992:Osmium; D005976:Glutaral