Mutants of nonproducer cell lines transformed by murine sarcoma viruses. I. Induction, isolation, particle production, and tumorigenicity.
A variety of cell mutants were obtained by a single 5'-bromodeoxyuridine (BrdU) treatment of an nonproducer (NP) cell line transformed by the Kirsten strain of murine sarcoma virus (Ki-MSV). Isolation procedures of these cell See PDF for Structure mutants are described. The cell mutants obtained were classified by tumorigenic potential and shedding of Type C virus particles. The cell mutants were classified into four groups: (A) tumorigenic, without particles; (B) tumorigenic, with Type C particles; (C) nontumorigenic, without particles; and (D) nontumorigenic, with Type C particles. The tumorigenic cell lines showed variability in morphology with both flat and typical transformed appearing cell lines showing equal transplantability.
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells; D017988:Gammaretrovirus; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D009154:Mutation / Q000187:drug effects; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental; D012190:Retroviridae / Q000302:isolation & purification; D012509:Sarcoma / Q000382:microbiology*; D014184:Transplantation, Homologous
D001973:Bromodeoxyuridine