On the pharmacology of ascending, decending and recurrent postsynaptic inhibition of the cuneo-thalamic relay cells in the cat.
1. In cats decerebrated or anaesthetized with pentobarbitone, cells of the middle third of the cuneate nucleus that were excited by tactile stimulation of the ipsilateral forelimb (responding to displacement of hairs, skin or joints) and inhibited by electrical stimulation of the contralateral pyramid, were invariably inhibited by electrical stimulation of the ipsilateral forepaw and the contralateral forelimb nerves.2. In 50% of the cats, the cells were more fully identified by placing electrodes stereotaxically in the contralateral medial lemniscus. Recurrent inhibition was always a concomitant of the antidromic action potential.3. The intensity and the duration of inhibition evoked by all of these pathways was totally resistant to iontophoretic and intravenous strychnine in doses at least 5 times that required to block completely the response of the same cells to iontophoretic glycine and was extremely sensitive to either iontophoretic bicuculline or picrotoxin.4. Although the inhibition was invariably sensitive to intravenous picrotoxin, no significant change occurred in the duration or intensity of the inhibition when bicuculline was administered intravenously (5 or 6 times) as repeated doses of 0.2 mg/kg.5. Postsynaptic inhibition in the cuneate may be mediated by gamma-aminobutyric acid released from the nerve terminals of a common pool of interneurones shared by ascending, descending and recurrent pathways. Since the receptors involved in this pathway are resistant to intravenous bicuculline, they may well be distinct from those responsible for changes in the primary afferent terminal excitability, usually believed to be associated with presynaptic inhibition.
D000200:Action Potentials; D000613:Aminobutyrates / Q000494:pharmacology; D000818:Animals; D002415:Cats; D003201:Computers; D004558:Electric Stimulation; D004566:Electrodes; D005998:Glycine / Q000494:pharmacology; D007478:Iontophoresis; D009433:Neural Inhibition / Q000187:drug effects; D010812:Physical Stimulation; D010852:Picrotoxin / Q000494:pharmacology; D017981:Receptors, Neurotransmitter / Q000187:drug effects*; D013331:Strychnine / Q000494:pharmacology; D013788:Thalamus / Q000187:drug effects* / Q000502:physiology
D000613:Aminobutyrates; D005998:Glycine; D010852:Picrotoxin; D017981:Receptors, Neurotransmitter; D013331:Strychnine