Inhibition by diuretics of cyclic 3',5'-AMP-dependent protein kinase from toad bladder epithelium.
1. A cAMP-dependent protein kinase enzyme has been isolated from toad bladder epithelium.2. This enzyme catalyses the phosphorylation of histones; a reaction stimulated by 0.1 muM cAMP.3. Activity of this enzyme in the presence of 0.1 muM cAMP was significantly inhibited by 100 muM mercuderamide, bumetanide and frusemide. A small, though statistically insignificant inhibition was seen with the same concentrations of hydrochlorothiazide and ethacrynic acid.4. The half maximal inhibition was achieved with mercuderamide, 5 muM, and with frusemide and bumetanide 600 muM.
D000818:Animals; D001565:Benzoates / Q000494:pharmacology; D001693:Biological Transport, Active; D002024:Bufo marinus; D002082:Butylamines / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D004232:Diuretics / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells; D004848:Epithelium / Q000201:enzymology; D004976:Ethacrynic Acid / Q000494:pharmacology; D005665:Furosemide / Q000494:pharmacology; D006016:Glycolates / Q000494:pharmacology; D006852:Hydrochlorothiazide / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D009941:Organomercury Compounds / Q000494:pharmacology; D010647:Phenyl Ethers / Q000494:pharmacology; D010761:Phosphorus Radioisotopes; D011422:Propionates / Q000494:pharmacology; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000378:metabolism; D001743:Urinary Bladder / Q000201:enzymology* / Q000378:metabolism
D001565:Benzoates; D002082:Butylamines; D004232:Diuretics; D006016:Glycolates; D009941:Organomercury Compounds; D010647:Phenyl Ethers; D010761:Phosphorus Radioisotopes; D011422:Propionates; D047428:Protein Kinase Inhibitors; D006852:Hydrochlorothiazide; D005665:Furosemide; D000242:Cyclic AMP; D011494:Protein Kinases; D004976:Ethacrynic Acid