title: Size and base composition of RNA in supercoiled plasmid DNA.
abstract: The average size and base composition of the covalently integrated RNA segment in supercoiled ColE(1) DNA synthesized in Escherichia coli in the presence of chloramphenicol (CM-ColE(1) DNA) have been determined by two independent methods. The two approaches yielded similar results, indicating that the RNA segment in CM-ColE(1) DNA contains GMP at the 5' end and comprises on the average 25 to 26 ribonucleotides with a base composition of 10-11 G, 3 A, 5-6 C, and 6-7 U.
mesh_terms: D000249:Adenosine Monophosphate / Q000378:metabolism; D000468:Alkalies / Q000378:metabolism; D001358:Avian Sarcoma Viruses / Q000201:enzymology; D001483:Base Sequence; D002701:Chloramphenicol / Q000494:pharmacology; D003597:Cytosine Nucleotides / Q000378:metabolism; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis* / Q000096:biosynthesis / Q000378:metabolism; D004590:Electrophoresis, Paper; D004926:Escherichia coli / Q000032:analysis* / Q000166:cytology / Q000201:enzymology; D006150:Guanine Nucleotides / Q000378:metabolism; D002479:Inclusion Bodies / Q000032:analysis; D009711:Nucleotides / Q000032:analysis; D010761:Phosphorus Radioisotopes; D011118:Polynucleotide Ligases / Q000378:metabolism; D012329:RNA, Bacterial / Q000032:analysis*; D012260:Ribonucleases / Q000378:metabolism; D012265:Ribonucleotides / Q000032:analysis*; D013942:Thymine Nucleotides / Q000378:metabolism
chemical_list: D000468:Alkalies; D003597:Cytosine Nucleotides; D004269:DNA, Bacterial; D006150:Guanine Nucleotides; D009711:Nucleotides; D010761:Phosphorus Radioisotopes; D012329:RNA, Bacterial; D012265:Ribonucleotides; D013942:Thymine Nucleotides; D000249:Adenosine Monophosphate; D002701:Chloramphenicol; D004254:DNA Nucleotidyltransferases; D012260:Ribonucleases; D011118:Polynucleotide Ligases