The metabolism of benzene by bacteria. Purification and some properties of the enzyme cis-1,2-dihydroxycyclohexa-3,5-diene (nicotinamide adenine dinucleotide) oxidoreductase (cis-benzene glycol dehydrogenase).
1. cis-Benzene glycol dehydrogenase was purified to a homogeneous state from a species of Pseudomonas grown with benzene as the major carbon source. 2. The enzyme was specific for the cis-isomer of its substrate and required NAD(+) as hydrogen acceptor. 3. Partial inactivation of the enzyme, which was observed during purification, could be reversed by the addition of Fe(2+) and GSH. 4. A molecular weight of 440000 was calculated from data obtained by sedimentation-velocity and diffusion analysis in the ultracentrifuge. Sodium dodecyl sulphate polyacrylamide-gel electrophoresis indicated a subunit of molecular weight 110000. 5. p-Chloromercuribenzoic acid and 1,10-phenanthroline were shown to inhibit the enzyme.
D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors; D001554:Benzene / Q000378:metabolism*; D002474:Cell-Free System; D002729:Chloromercuribenzoates / Q000494:pharmacology; D002848:Chromatography, DEAE-Cellulose; D003511:Cyclohexanols; D004058:Diffusion; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D005978:Glutathione / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000494:pharmacology; D007536:Isomerism; D008970:Molecular Weight; D009243:NAD; D010088:Oxidoreductases / Q000302:isolation & purification*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D010618:Phenanthrolines / Q000494:pharmacology; D011549:Pseudomonas / Q000201:enzymology*; D012967:Sodium Dodecyl Sulfate; D014461:Ultracentrifugation
D002729:Chloromercuribenzoates; D003511:Cyclohexanols; D010618:Phenanthrolines; D009243:NAD; D012967:Sodium Dodecyl Sulfate; D007501:Iron; D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C020054:cis-1,2-dihydrobenzene-1,2-diol dehydrogenase; D005978:Glutathione; D001554:Benzene