Improved hemagglutination inhibition assay for mammary tumor virus antigen.
It is our experience that the use of sheep red blood cells pretreated with formaldehyde and pyruvaldehyde before sensitizing with viral antigen yields results that are comparable to that obtained by using the tannic acid method. The double aldehyde method provides the added advantage of being useful for assaying the viral antigen content in substances which contain high levels of interfering substances which would preclude the use of other assay methods.
D000447:Aldehydes; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D004912:Erythrocytes / Q000276:immunology; D005069:Evaluation Studies as Topic; D005557:Formaldehyde; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007106:Immune Sera; D008324:Mammary Tumor Virus, Mouse / Q000276:immunology*; D008722:Methods; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D008892:Milk / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D012756:Sheep / Q000276:immunology; D013634:Tannins
D000447:Aldehydes; D000956:Antigens, Viral; D007106:Immune Sera; D013634:Tannins; D005557:Formaldehyde