Demonstration of a glucocorticoid hormone-receptor complex in the cytoplasm of a hormone-responsive tumour.
Specific substances binding [(3)H]-triamcinolone acetonide have been demonstrated in the cytoplasmic fraction of the R3230AC mammary carcinoma using sucrose gradient centrifugation. These receptors, which exhibit sedimentation coefficients of 8-9 Svedberg units, are relatively specific for glucocorticoid ligands and possess a high affinity for triamcinolone acetonide (K(d)∼7 × 10(-8) mol/1).
D000818:Animals; D001665:Binding Sites; D002499:Centrifugation, Density Gradient; D003593:Cytoplasm / Q000378:metabolism*; D003900:Desoxycorticosterone / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D005260:Female; D006854:Hydrocortisone / Q000378:metabolism; D008024:Ligands; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface; D013395:Sucrose; D014222:Triamcinolone Acetonide / Q000378:metabolism*; D014316:Tritium
D008024:Ligands; D011956:Receptors, Cell Surface; D014316:Tritium; D003900:Desoxycorticosterone; D011374:Progesterone; D004958:Estradiol; D013395:Sucrose; D014222:Triamcinolone Acetonide; D006854:Hydrocortisone