Three-day assay for human cytomegalovirus applicable to serum neutralization tests.
A fluorescing cell assay (FCA) technique utilizing the indirect fluorescent-antibody method to measure human cytomegalovirus (CMV)-infected cells has been applied to the rapid determination of CMV-neutralizing antibody. Human sera with complement fixation titers to CMV of 1/32 or greater and fluorescein-conjugated rabbit anti-human globulin are the primary and secondary reagents in the fluorescent-antibody test. FCA measured in 3 days the same number of infectious units measured by plaque assay in 2 weeks. FCA and plaque assay yielded identical neutralizing antibody titers to CMV in 20 human sera.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002452:Cell Count; D003168:Complement Fixation Tests; D046508:Culture Techniques; D003587:Cytomegalovirus / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification*; D003588:Cytopathogenic Effect, Viral; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera; D008722:Methods; D008856:Microscopy, Fluorescence; D009500:Neutralization Tests / Q000592:standards*; D011817:Rabbits / Q000276:immunology; D010948:Viral Plaque Assay
D000956:Antigens, Viral; D007106:Immune Sera