Integration of deoxyribonucleic acid specific for Rous sarcoma virus after infection of permissive and nonpermissive hosts.
A relatively simple but stringent technique was developed to detect the integration of virus-specific DNA into the genomes of higher organisms. In both permissive (duck) and nonpermissive (mammalian) cells which normally contain no nucleotide sequences specific for Rous sarcoma virus, transformation by the virus results in the appearance of DNA specific for Rous sarcoma virus covalently integrated into strands of host-cell DNA containing reiterated sequences. Early after infection of mouse or duck cells by Rous sarcoma virus, unintegrated DNA specific for the virus can be demonstrated.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000378:metabolism*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000032:analysis; D004254:DNA Nucleotidyltransferases; D004279:DNA, Viral / Q000096:biosynthesis* / Q000302:isolation & purification; D004372:Ducks; D004622:Embryo, Mammalian; D004625:Embryo, Nonmammalian; D005347:Fibroblasts; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D009691:Nucleic Acid Denaturation; D009695:Nucleic Acid Renaturation; D014316:Tritium
D004279:DNA, Viral; D014316:Tritium; D004247:DNA; D004254:DNA Nucleotidyltransferases