The characterization of a non-histone protein isolated from histone F1 preparations.
A non-histone protein was obtained by extraction of nuclei derived from rat liver or thymus or ascites-hepatoma cells with 5% (w/v) HClO(4). Separation from histone F1 was achieved by chromatography on DEAE-cellulose. The purified component P1 was characterized and the formation of complexes with histone F1 and polylysine was studied.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D002467:Cell Nucleus / Q000032:analysis; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000032:analysis* / Q000302:isolation & purification; D008099:Liver / Q000032:analysis / Q000166:cytology; D008113:Liver Neoplasms; D008970:Molecular Weight; D009698:Nucleoproteins / Q000032:analysis* / Q000302:isolation & purification; D010710:Phosphates / Q000032:analysis; D010759:Phosphorus Isotopes; D011485:Protein Binding; D051381:Rats; D013053:Spectrophotometry; D013950:Thymus Gland / Q000032:analysis / Q000166:cytology
D000596:Amino Acids; D006657:Histones; D009698:Nucleoproteins; D010710:Phosphates; D010759:Phosphorus Isotopes