Interaction of phalloidin with actin.
Phalloidin, a toxic bicyclic peptide of rapid action from the toadstool, Amanita phalloides, gives rise to polymerization of G-actin to filamentous structures (Ph-actin) in a medium of low ionic strength. Ph-actin closely resembles the microfilaments found in liver membrane fractions (Ph-filaments) after in vivo or in vitro poisoning. Both phalloidin induced filaments are resistant to 0.6 M KI in contrast to F-actin, and become decorated by heavy meromyosin. After preincubation with cytochalasin B significantly fewer actin filaments are observed.
D000199:Actins / Q000378:metabolism*; D001487:Basidiomycota; D002458:Cell Fractionation; D002462:Cell Membrane / Q000187:drug effects; D003571:Cytochalasin B / Q000494:pharmacology; D002479:Inclusion Bodies / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D009183:Mycotoxins / Q000378:metabolism*; D015879:Myosin Subfragments / Q000494:pharmacology; D010456:Peptides, Cyclic / Q000378:metabolism*; D010936:Plant Extracts; D011193:Potassium Iodide / Q000494:pharmacology
D000199:Actins; D009183:Mycotoxins; D015879:Myosin Subfragments; D010456:Peptides, Cyclic; D010936:Plant Extracts; D011193:Potassium Iodide; D003571:Cytochalasin B