title: The mechanism of viral replication. Structure of replication complexes of encephalomyocarditis virus.
abstract: The structure of the purified replicative intermediate of encephalomyocarditis virus was determined by electron microscopy. Approximately 80% of the replicative intermediate complexes were characterized by a filament of double-stranded RNA of widely variable length, which had a "bush" of single-stranded RNA at one end. In many examples one or more additional single-stranded bushes were appended internally to the double-stranded RNA filament. These results support the view that before deproteinization, replicative intermediate contains little if any double-stranded RNA.
mesh_terms: D002499:Centrifugation, Density Gradient; D004686:Encephalomyocarditis virus / Q000378:metabolism*; D008854:Microscopy, Electron; D008954:Models, Biological; D012367:RNA, Viral / Q000096:biosynthesis*; D014779:Virus Replication
chemical_list: D012367:RNA, Viral