Partial transcription of murine type C viral genomes in BALB c cell lines.
The mouse cell line, BALB/c 3T3, and its derivatives transformed either spontaneously or by treatment with a variety of external agents, were analyzed for cytoplasmic RNA complementary to DNA products prepared from the Kirsten strain of murine sarcoma-leukemia virus, and from an endogenous type C virus of BALB/c 3T3. Although none of these cell lines spontaneously releases complete type C virions, they all contain RNA which is partially homologous to a portion of the 35S RNA isolated from these viruses. The parental cell line, BALB/c 3T3, contains a low level of viral-related RNA, and there is an increased amount of this RNA in some of the transformed cells. The RNA detected represents only a fraction of the viral RNA found in virus-producing cells. The formation of RNA:DNA hybrids was detected by equilibrium centrifugation in Cs(2)SO(4) density gradients and by analysis with a single-strand-specific nuclease from Aspergillus oryzae. Viral DNA products prepared either from an endogenous reaction with whole virus in the presence of actinomycin D or from purified 70S viral RNA as template using avian myeloblastosis virus DNA polymerase yield comparable data. In addition, all of the BALB/c lines examined produce detectable levels of murine type C virus group-specific antigen.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D002848:Chromatography, DEAE-Cellulose; D004254:DNA Nucleotidyltransferases; D004279:DNA, Viral / Q000378:metabolism*; D003851:Deoxyribonucleases; D009052:Leukemia Virus, Murine / Q000032:analysis / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009693:Nucleic Acid Hybridization; D012335:RNA, Ribosomal; D012367:RNA, Viral / Q000096:biosynthesis* / Q000302:isolation & purification; D014158:Transcription, Genetic; D014779:Virus Replication
D000956:Antigens, Viral; D004279:DNA, Viral; D012335:RNA, Ribosomal; D012367:RNA, Viral; D004254:DNA Nucleotidyltransferases; D003851:Deoxyribonucleases