Radioimmunoassay of mammalian type C viral proteins. 3. Detection of viral antigen in normal murine cells and tissues.
A radioimmunoassay specific for a murine leukemia virus structural protein, the gs antigen, detects an antigenic reactivity in normal murine cells in culture and natural tissues. The assay was shown to measure an antigen that is highly related to the virion protein as shown by absorption tests, immunoadsorbent chromatography, and by analysis of linearized dose-response curves. These findings combined with the finding of viral-specific RNA indicate that portions of the viral genome are being expressed with a much greater frequency than previously appreciated.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D002478:Cells, Cultured / Q000032:analysis; D002846:Chromatography, Affinity; D005260:Female; D006801:Humans; D007456:Iodine Isotopes; D007668:Kidney / Q000276:immunology*; D009052:Leukemia Virus, Murine / Q000276:immunology*; D008099:Liver / Q000276:immunology*; D008221:Lymphoid Tissue / Q000276:immunology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis; D011863:Radioimmunoassay; D051381:Rats; D011925:Rauscher Virus / Q000276:immunology; D012190:Retroviridae / Q000276:immunology; D013936:Thymidine; D014316:Tritium; D014764:Viral Proteins / Q000032:analysis*
D000956:Antigens, Viral; D007456:Iodine Isotopes; D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium; D013936:Thymidine