Studies on bone matrix glycoproteins. Incorporation of (1-14C)glucosamine and plasma (14C)glycoprotein into rabbit cortical bone.
The radioactively labelled constituents present in bone matrix were compared 12 days after injection of either [(14)C]glucosamine or plasma [(14)C]glycoprotein. Both precursors are utilized in the synthesis of organic matrix by bone tissue. Cortical bone from animals injected with [(14)C]glucosamine contains radioactivity derived from glucosamine and plasma glycoproteins and all glycoprotein fractions are labelled. Plasma [(14)C]glycoprotein labels the less acidic glycoproteins to a greater extent than the more acidic components. An antibody has been raised against the less-acidic-glycoprotein fraction of bone. The latter contains a glycoprotein of alpha-mobility that appears to be concentrated specifically in bone tissue and which is present also in plasma. This alpha-glycoprotein accounts for a large proportion of the components labelled and retained in bone matrix after [(14)C]glucosamine injection.
D000418:Albumins / Q000378:metabolism; D000818:Animals; D000906:Antibodies; D001797:Blood Protein Electrophoresis; D001798:Blood Proteins / Q000378:metabolism; D001810:Blood Volume; D001857:Bone Matrix / Q000276:immunology / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D005944:Glucosamine / Q000378:metabolism*; D006023:Glycoproteins / Q000097:blood*; D006041:Goats / Q000276:immunology; D006168:Guinea Pigs / Q000276:immunology; D007106:Immune Sera; D007122:Immunoelectrophoresis; D003012:Microbial Collagenase; D011817:Rabbits; D012709:Serum Albumin / Q000378:metabolism; D012756:Sheep / Q000276:immunology
D000418:Albumins; D000906:Antibodies; D001798:Blood Proteins; D002250:Carbon Radioisotopes; D006023:Glycoproteins; D007106:Immune Sera; D012709:Serum Albumin; D003012:Microbial Collagenase; D005944:Glucosamine