title: The metabolism of nitrilotriacetate by a pseudomonad.
abstract: 1. An organism that grows on nitrilotriacetate as sole source of carbon and energy was isolated in pure culture and was identified as a pseudomonad. 2. Cell-free extracts of the nitrilotriacetate-grown pseudomonad contain an enzyme that catalyses the NADH-and O(2)-dependent oxidation of nitrilotriacetate to iminodiacetate and glyoxalate. This enzyme is absent from extracts of glucose-grown cells. 3. Compared with growth on glucose, growth on nitrilotriacetate results in increased activities of enzymes of glycine and serine metabolism, namely serine hydroxymethyltransferase, glycine decarboxylase, serine-oxaloacetate aminotransferase and hydroxypyruvate reductase. 4. Cell-free extracts of the nitrilotriacetate-grown organism contain the enzyme glyoxalate carboligase and, when supplemented with NADH, Mg(2+) and thiamin pyrophosphate, can catalyse the anaerobic conversion of glyoxalate into glycerate. 5. These results are incorporated in a scheme which shows the oxidative metabolism of nitrilotriacetate by the successive removal of C(2) units to form 2mol of glyoxalate and 1mol of glycine per mol of nitrilotriacetate degraded. The glyoxalate and glycine are then both metabolized to glycerate by separate pathways, via tartronic semialdehyde and serine respectively. The role of this scheme in the growth of the organism on nitrilotriacetate is discussed.
mesh_terms: D000085:Acetates / Q000378:metabolism*; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D000693:Anaerobiosis; D002262:Carboxy-Lyases / Q000378:metabolism; D002474:Cell-Free System; D003470:Culture Media; D003902:Detergents / Q000378:metabolism*; D003998:Dicarboxylic Acids / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005988:Glyceric Acids / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D006038:Glyoxylates / Q000378:metabolism; D008025:Ligases / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D009243:NAD; D009571:Nitrilotriacetic Acid / Q000378:metabolism; D010071:Oxaloacetates; D010084:Oxidation-Reduction; D010100:Oxygen; D011549:Pseudomonas / Q000378:metabolism*; D011773:Pyruvates; D012694:Serine / Q000378:metabolism; D013763:Tetrahydrofolates; D013835:Thiamine Pyrophosphate / Q000378:metabolism; D000637:Transaminases / Q000378:metabolism; D014166:Transferases / Q000378:metabolism
chemical_list: D000085:Acetates; D000596:Amino Acids; D003470:Culture Media; D003902:Detergents; D003998:Dicarboxylic Acids; D005988:Glyceric Acids; D006038:Glyoxylates; D010071:Oxaloacetates; D011773:Pyruvates; D013763:Tetrahydrofolates; D009243:NAD; D012694:Serine; D000429:Alcohol Oxidoreductases; D014166:Transferases; D000637:Transaminases; D002262:Carboxy-Lyases; D008025:Ligases; D008274:Magnesium; D005947:Glucose; D009571:Nitrilotriacetic Acid; D013835:Thiamine Pyrophosphate; D010100:Oxygen; D005998:Glycine