The synthesis in vitro of RNA polymerase subunits of Escherichia coli.
DNA from transducing bacteriophage that carry the structural gene for the beta subunit of RNA polymerase has been used to direct protein synthesis in a cell-free transcription-translation system. A polypeptide species has been detected which appears to be beta-subunit material made in vitro.
D002474:Cell-Free System; D003090:Coliphages / Q000032:analysis; D000242:Cyclic AMP / Q000494:pharmacology; D004279:DNA, Viral / Q000302:isolation & purification / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000032:analysis / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D008970:Molecular Weight; D014176:Protein Biosynthesis; D013698:Templates, Genetic; D014158:Transcription, Genetic; D014316:Tritium
D004279:DNA, Viral; D014316:Tritium; D000242:Cyclic AMP; D012321:DNA-Directed RNA Polymerases