Dexamethasone stimulation of murine mammary tumor virus expression: a tissue culture source of virus.
In mouse cell lines derived from mammary adenocarcinomnas, the synthetic steroid dexamnethasone stimulates production of murine mammary tumor virus. Viral RNA and antigens are increased as much as 20-fold, and culture fluid supernatants from steroid-treated cells contain type B particles with reverse transcriptase. These cells provide a possible tissue culture source of this virus and a model system for studying the mechanism of action of corticosteroids and the regulation of transcription of integrated viral DNA.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D002999:Clone Cells; D004279:DNA, Viral; D003907:Dexamethasone / Q000494:pharmacology*; D005260:Female; D005455:Fluorescent Antibody Technique; D008325:Mammary Neoplasms, Experimental / Q000382:microbiology*; D008324:Mammary Tumor Virus, Mouse / Q000254:growth & development* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008854:Microscopy, Electron; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis; D013268:Stimulation, Chemical; D014158:Transcription, Genetic / Q000187:drug effects; D014764:Viral Proteins / Q000096:biosynthesis; D014776:Virus Cultivation; D014779:Virus Replication / Q000187:drug effects*
D000956:Antigens, Viral; D004279:DNA, Viral; D012367:RNA, Viral; D014764:Viral Proteins; D003907:Dexamethasone