A new chromatographic system for vitamin D3 and its metabolites: resoluation of a new vitamin D3 metabolite.
A simple yet powerful new chromatographic procedure for vitamin D(3) and its metabolites is described. Liquid-gel partition chromatography on Sephadex LH-20 using a solvent of various percentages of CHCl(3) in Skellysolve B (petroleum ether, bp 67-69 degrees C) permits excellent resolution of vitamin D(3), 25-hydroxyvitamin D(3), and their more polar metabolites. Of special importance is the resolution of the metabolites of vitamin D(3) more polar than 25-hydroxycholecalciferol. Because of this resolution, a new metabolite of vitamin D(3) has been demonstrated in the plasma of rats and in the intestines of chicks given 100 IU of vitamin D(3)-1,2-(3)H.
D000818:Animals; D002645:Chickens; D002762:Cholecalciferol / Q000097:blood* / Q000378:metabolism; D002845:Chromatography; D002850:Chromatography, Gel; D004100:Dihydroxycholecalciferols / Q000097:blood; D005069:Evaluation Studies as Topic; D006887:Hydroxycholecalciferols / Q000096:biosynthesis / Q000097:blood; D007421:Intestine, Small / Q000378:metabolism; D008722:Methods; D051381:Rats; D012822:Silicon Dioxide; D014316:Tritium; D014808:Vitamin D Deficiency / Q000378:metabolism
D004100:Dihydroxycholecalciferols; D006887:Hydroxycholecalciferols; D014316:Tritium; D002762:Cholecalciferol; D012822:Silicon Dioxide