An improved cell fractionation procedure for the preparation of rat liver membrane-bound ribosomes.
A cell fractionation procedure is described which allows the preparation from rat liver of a rough microsome population containing almost 50% of the membrane-bound ribosomes of the tissue. The fraction is not contaminated with free ribosomes or smooth microsomes, and, by various other criteria, is suitable for studies of ribosome-membrane interaction.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002458:Cell Fractionation; D002462:Cell Membrane; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000032:analysis; D003576:Electron Transport Complex IV / Q000032:analysis; D004721:Endoplasmic Reticulum / Q000032:analysis; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology* / Q000201:enzymology; D008297:Male; D008722:Methods; D008862:Microsomes, Liver / Q000032:analysis / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis; D011506:Proteins / Q000032:analysis; D012313:RNA / Q000032:analysis; D051381:Rats; D012270:Ribosomes; D013056:Spectrophotometry, Ultraviolet; D013395:Sucrose; D013997:Time Factors; D014316:Tritium
D000596:Amino Acids; D010743:Phospholipids; D011506:Proteins; D014316:Tritium; D013395:Sucrose; D012313:RNA; D004247:DNA; D003576:Electron Transport Complex IV