title: Mutagenesis and enrichment of auxotrophs in Penicillium chrysogenum.
abstract: An efficient method for isolation of auxotrophs of Penicillium chyrysogenum involving mutagenesis with ethyl methanesulfonate followed by enrichment with sodium pentachlorophenate was developed. The auxotroph frequencies obtained were 30 to 40%.
mesh_terms: D000476:Alkanesulfonates; D003470:Culture Media; D008239:Lysine / Q000378:metabolism; D008722:Methods; D008851:Micropore Filters; D009153:Mutagens; D009154:Mutation; D010407:Penicillium / Q000302:isolation & purification*; D010408:Penicillium chrysogenum / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D010416:Pentachlorophenol; D013172:Spores, Fungal / Q000254:growth & development
chemical_list: D000476:Alkanesulfonates; D003470:Culture Media; D009153:Mutagens; D010416:Pentachlorophenol; D008239:Lysine