Sensitive in vivo assay for detection of murine leukemia viruses.
A test is reported for the in vivo detection of the replicative ability of murine leukemia viruses (MLV) in the BALB/c mouse strain. Growth in the spleen was assayed by the complement-fixation assay for MLV group-specific antigen after injection of newborn mice. Low doses of laboratory-derived and wild-type MLV from cell-culture and animal-grown sources were detected as early as 7 to 14 days postinoculation. The sensitivity of this in vivo test compared favorably with in vitro assays for MLV. In vivo detection of MLV replication was correlated with long-term oncogenicity.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D003168:Complement Fixation Tests; D005069:Evaluation Studies as Topic; D005347:Fibroblasts; D009052:Leukemia Virus, Murine / Q000254:growth & development* / Q000276:immunology; D007942:Leukemia, Experimental / Q000453:epidemiology; D008722:Methods; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000276:immunology / Q000382:microbiology*; D014779:Virus Replication
D000956:Antigens, Viral