Purification of mouse interferon by affinity chromatography on a solid-phase immunoadsorbent.
A solid-phase immunoadsorbent capable of binding mouse interferon has been prepared. Starting from crude tissue-culture material, interferon could be purified 1990 times in a single step of affinity chromatography. Overall recovery ranged from 55 to 103% with tissue culture and mouse-brain interferon; however, only 5% was obtained with Sendai virus-induced interferon from mouse serum.
D000818:Animals; D002478:Cells, Cultured; D002845:Chromatography; D002846:Chromatography, Affinity; D007106:Immune Sera; D007136:Immunoglobulins; D007372:Interferons / Q000096:biosynthesis / Q000302:isolation & purification*; D008722:Methods; D051379:Mice; D008810:Mice, Inbred C57BL; D009522:Newcastle disease virus; D010222:Parainfluenza Virus 1, Human; D011134:Polysaccharides; D012756:Sheep / Q000276:immunology
D007106:Immune Sera; D007136:Immunoglobulins; D011134:Polysaccharides; D007372:Interferons