Separation of isozymes of horse liver alcohol dehydrogenase and purification of the enzyme by affinity chromatography on an immobilized AMP-analogue.
D000249:Adenosine Monophosphate / Q000031:analogs & derivatives; D000362:Agar; D000429:Alcohol Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002846:Chromatography, Affinity; D004586:Electrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D000431:Ethanol; D006736:Horses; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D009243:NAD; D011485:Protein Binding; D011720:Pyrazoles; D012685:Sepharose; D013056:Spectrophotometry, Ultraviolet
D007527:Isoenzymes; D011720:Pyrazoles; D009243:NAD; D000431:Ethanol; D000249:Adenosine Monophosphate; D000362:Agar; D012685:Sepharose; D000429:Alcohol Oxidoreductases