Analysis of alpha-lipoprotein cholesterol in 50 microl of plasma.
A micromethod for quantitation of alpha-lipoprotein cholesterol was devised for gas-liquid chromatography to minimize plasma sample size and facilitate lipid studies using capillary blood from children or small animals. alpha-Lipoprotein cholesterol was measured by gas-liquid chromatography in 20 micro l of the centrifuged supernate obtained after addition of 5 micro l of mixed heparin-manganese chloride solution 1:1 (v/v) to 50 micro l of plasma. Comparison of venous alpha-lipoprotein cholesterol measured by gas-liquid chromatography with venous alpha-lipoprotein cholesterol measured by conventional heparin-manganese precipitation and ferric chloride (colorimetric) cholesterol determination gave a correlation coefficient of 0.98 for 80 plasma samples. Capillary alpha-lipoprotein cholesterol and venous alpha-lipoprotein cholesterol were closely correlated in 31 patients (r = 0.97).
D000328:Adult; D000818:Animals; D002197:Capillary Action; D002784:Cholesterol / Q000097:blood*; D002849:Chromatography, Gas; D003124:Colorimetry; D005069:Evaluation Studies as Topic; D006493:Heparin; D006801:Humans; D007202:Indicators and Reagents; D007231:Infant, Newborn; D007501:Iron; D008075:Lipoproteins, HDL / Q000097:blood*; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D008345:Manganese; D008722:Methods; D008832:Microchemistry
D007202:Indicators and Reagents; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D008345:Manganese; D006493:Heparin; D002784:Cholesterol; D007501:Iron