Secretion of apolipoproteins in very low density and high density lipoproteins by perfused rat liver.
The incorporation of labeled amino acids into the peptides of very low density lipoproteins (VLDL) and high density lipoproteins (HDL) secreted by perfused rat liver was studied using a Ringer-albumin solution in the perfusate in place of serum to diminish exchange of peptides between VLDL and HDL. Among the lipoproteins, the greatest release of protein, greatest incorporation of amino acid, and highest specific activity were found in VLDL. After separation of the delipidated peptides by electrophoresis on polyacrylamide gel, the incorporation into VLDL peptides was found to be 5-10 times as great as into HDL peptides. There was virtually no incorporation into the peptides of low density lipoproteins (LDL). Approximately 25% of the radioactivity incorporated into perfusate VLDL failed to enter the 13% polyacrylamide gel. The remaining radioactivity was distributed primarily among three peptide bands; one, found in the upper portion of the gel, contained 45% of the total, most of the remainder being found in two rapidly migrating bands. These three peptides appear to approximate those of human apo-C in relative electrophoretic mobility. Most of the HDL peptide radioactivity entering the running gel was found in a band that migrates slightly faster than the main VLDL band. A portion of the radioactivity of this major HDL band did not enter the running gel unless beta-mercaptoethanol was present. Greater separation of these two bands by polyacrylamide gel electrophoresis for 24 hr confirmed that the major bands in VLDL and in HDL were different. The rapidly moving peptides of HDL were found to contain very little radioactivity. Determination of the intensity of staining of carrier-free perfusate VLDL and HDL peptides produced a pattern similar to the incorporation of labeled amino acids. It is concluded that the rapidly moving peptides, which may contain activators of lipoprotein lipase, are only secreted as part of the VLDL.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001059:Apoproteins / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D007930:Leucine / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008079:Lipoproteins, VLDL / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D008623:Mercaptoethanol; D010455:Peptides / Q000378:metabolism; D010477:Perfusion; D051381:Rats; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D014316:Tritium; D014461:Ultracentrifugation; D014633:Valine / Q000378:metabolism
D000596:Amino Acids; D001059:Apoproteins; D002250:Carbon Radioisotopes; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D010455:Peptides; D014316:Tritium; D012710:Serum Albumin, Bovine; D008623:Mercaptoethanol; D007930:Leucine; D014633:Valine