Dibutyryl cyclic adenosine 3'5' monophosphate, sugar transport, and regulatory control of cell division in normal and transformed cells.
Swiss 3T3 cells exhibit contact-regulated cell growth and have a lower ability to transport 2-deoxyglucose than polyoma (Py)-transformed 3T3 cells. Py3T3 cells treated with dibutyryl cyclic adenosine 3'5' monophosphate (dBcAMP) and theophylline have reduced cell growth and transport 2-deoxyglucose at the same rate as normal 3T3 cells. Evidence that the cessation of cell growth and reduced transport abilities in Py3T3 cells does not represent a return to contact-regulated growth comes from the following observations. First, treating high density Py3T3 cells with dBcAMP allows more than two doublings of cell number, even though ability to transport 2-deoxyglucose is returned to levels equal to those of normal 3T3 cells. Second, dBcAMP prevents serum-stimulated increases in 2-deoxyglucose transport in Py3T3 but not in 3T3 cells.
D000371:Agglutination / Q000187:drug effects; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002087:Butyrates; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002471:Cell Transformation, Neoplastic; D003260:Contact Inhibition / Q000187:drug effects; D003470:Culture Media; D000242:Cyclic AMP / Q000378:metabolism* / Q000494:pharmacology; D005347:Fibroblasts; D005947:Glucose / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains / Q000196:embryology; D011120:Polyomavirus; D013806:Theophylline / Q000494:pharmacology; D014316:Tritium
D002087:Butyrates; D003470:Culture Media; D014316:Tritium; D013806:Theophylline; D000242:Cyclic AMP; D005947:Glucose