Proteins specified by herpes simplex virus. XII. The virion polypeptides of type 1 strains.
The polypeptides from purified virions of a herpes simplex 1 (human herpes-virus 1) strain, F1, which had been passaged a limited number of times in cell culture after isolation, formed 33 bands on electrophoretic separation in polyacrylamide gels cross-linked with N, N'-diallyltartardiamide in contrast to a maximum resolution of only 24 to 25 bands in gels cross-linked with N, N'-methylenebisacrylamide. This increase in the number of bands was due chiefly to an improved separation of glycosylated polypeptides from nonglycosylated polypeptides with which they co-electrophoresed on methylenebisacrylamide cross-linked gels. Purified virions of HSV-1 [F1] had a protein/DNA mass ratio of 10.7 +/- 0.96, and based on a DNA molecular mass of 85 x 10(6) to 100 x 10(6) the estimated weight of virion polypeptides ranges from 16.4 to 19.4 x 10(-16) g. The number of molecules of each polypeptide per virion ranged from less than 50 to 1,500. Comparison of the virion polypeptides of two HSV-1 strains with similar isolation and limited passage history with those of four HSV-1 strains with histories of numerous passages outside the human host showed a number of nonrandom variations in virion polypeptides. Thus, although the virion polypeptides of two strains with similar isolation and limited passage history could not be differentiated, strains with extended passage histories differed markedly from each other and from the limited passage strains in the number and electrophoretic mobility of noncapsid polypeptides and notably in those of the envelope.
D001345:Autoradiography; D002250:Carbon Radioisotopes; D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005643:Fucose; D005944:Glucosamine; D006801:Humans; D066298:In Vitro Techniques; D007822:Laryngeal Neoplasms; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis*; D018139:Simplexvirus / Q000032:analysis*; D014764:Viral Proteins / Q000032:analysis
D002250:Carbon Radioisotopes; D004279:DNA, Viral; D010455:Peptides; D014764:Viral Proteins; D005643:Fucose; D005944:Glucosamine