Isolation of Escherichia coli RNA polymerase binding sites on T5 and T7 DNA: further evidence for sigma-dependent recognition of A-T-rich DNA sequences.
Previous isolation and analysis of E. coli RNA polymerase (EC 2.7.7.6) binding sites on lambda DNA had demonstrated the existence of a sigma-dependent process of recognition of A-T-rich DNA sequences. We have now extended this finding to T5 and T7 DNA and häve provided evidence for the double-strandedness of the isolated binding sites. The possible equation of these sites to the genetically defined promoters is discussed.
D000227:Adenine Nucleotides / Q000302:isolation & purification; D001483:Base Sequence; D001665:Binding Sites; D003090:Coliphages / Q000032:analysis*; D003597:Cytosine Nucleotides / Q000302:isolation & purification; D004279:DNA, Viral / Q000032:analysis*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D003851:Deoxyribonucleases; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases; D004926:Escherichia coli / Q000201:enzymology*; D006150:Guanine Nucleotides / Q000302:isolation & purification; D006868:Hydrolysis; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000302:isolation & purification* / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases; D010761:Phosphorus Radioisotopes; D013942:Thymine Nucleotides / Q000302:isolation & purification
D000227:Adenine Nucleotides; D003597:Cytosine Nucleotides; D004279:DNA, Viral; D006150:Guanine Nucleotides; D009841:Oligonucleotides; D010761:Phosphorus Radioisotopes; D013942:Thymine Nucleotides; D012321:DNA-Directed RNA Polymerases; D003851:Deoxyribonucleases; D004720:Endonucleases; D010727:Phosphoric Diester Hydrolases