Location of the T4 gene 32 protein binding site on simian virus 40 DNA.
The T4 gene 32 protein, which binds to single-stranded but not duplex DNA, forms a specifically located denaturation loop in covalently closed circular simian virus 40 (SV40) DNA. Cleavage of the SV40 DNA-gene 32 protein complex with a restriction endonuclease from Hemophilus parainfluenzae shows the loop center to be at 0.46 on the SV40 DNA map. This is within one of the regions of SV40 DNA cleaved preferentially by the single-strand-specific nuclease S(1).
D001665:Binding Sites; D003090:Coliphages; D004270:DNA, Circular; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D003851:Deoxyribonucleases / Q000378:metabolism; D005796:Genes; D006190:Haemophilus / Q000201:enzymology; D009691:Nucleic Acid Denaturation; D011485:Protein Binding; D013539:Simian virus 40 / Q000032:analysis*; D014764:Viral Proteins
D004270:DNA, Circular; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D014764:Viral Proteins; D003851:Deoxyribonucleases