Concentration of elongation factor 2 in rat skeletal muscle during protein depletion and re-feeding.
Rat skeletal-muscle elongation factor 2 was assayed by causing it to react with NAD(+) by using fragment A of diphtheria toxin as the catalyst. Dietary protein restriction decreased the concentration of elongation factor 2 in homogenates of whole muscle. These decreases paralleled a decline in muscle RNA so that the number of molecules of elongation factor 2 per ribosome appeared to be independent of the diet. We conclude that elongation factor 2 is probably not the factor limiting the rate of muscle protein synthesis and is not responsible for the fall in the protein-synthetic rate in vivo observed in the muscles of animals whose dietary protein intake is inadequate.
D000818:Animals; D001835:Body Weight; D004032:Diet; D008297:Male; D009124:Muscle Proteins / Q000032:analysis* / Q000096:biosynthesis; D009132:Muscles / Q000032:analysis*; D009243:NAD / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D011488:Protein Deficiency / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D051381:Rats
D009124:Muscle Proteins; D010761:Phosphorus Radioisotopes; D009243:NAD; D012313:RNA