Adrenoceptors and adenyl cyclase in gills.
The content of cyclic 3',5'-adenosine monophosphate (cAMP) in the gills of the mullet (Mugil capito) has been measured. The basal level was found to be 2.9+/-0.3 pmol/mg wet weight. When fish were first anaesthetized with MS222 (tricaine) and the gills perfused to remove blood, the concentration of cAMP was raised to 14.3+/-1.9 pmol/mg wet weight.Adrenaline caused a significant increase in cAMP content, an effect that was blocked by propranalol, a beta-adrenoceptor antagonist. In the presence of the alpha-adrenoceptor block- ing agent phentolamine, adrenaline still caused an increase in nucleotide content.The results are discussed in relation to adrenaline and the adenyl cyclase system.
D000262:Adenylyl Cyclases; D000818:Animals; D000242:Cyclic AMP / Q000032:analysis*; D004837:Epinephrine / Q000494:pharmacology; D005399:Fishes / Q000378:metabolism*; D005880:Gills / Q000033:anatomy & histology* / Q000201:enzymology; D010646:Phentolamine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D011941:Receptors, Adrenergic
D011941:Receptors, Adrenergic; D011433:Propranolol; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D004837:Epinephrine; D010646:Phentolamine