A new method for the preparation of uniformly 14C-labelled compounds by using Anacystis nidulans.
1. Growth conditions were devised which enabled Anacystis nidulans to be grown on high specific radioactivity (14)CO(2) (at over 98% isotopic abundance of (14)C) in a closed system with greater than 85% substrate utilization. 2. The DNA and RNA content of A. nidulans was estimated by various methods and compared with that of Chlorella pyrenoidosa. 3. A procedure was developed for the quantitative extraction and separation of the DNA and RNA from A. nidulans. 4. The fractionation and analysis of the DNA and RNA includes methods for the preparation of the uniformly (14)C-labelled deoxyribonucleoside monophosphates and the uniformly (14)C-labelled ribonucleoside monophosphates. 5. The base 6-methylaminopurine was identified as a component of the DNA. 6. The preparation and total yield of uniformly (14)C-labelled amino acids from A. nidulans are also briefly described. The results are discussed in relation to the use of other micro-organisms for the preparation of compounds labelled with (14)C.
D000249:Adenosine Monophosphate; D000596:Amino Acids / Q000302:isolation & purification; D002250:Carbon Radioisotopes; D002708:Chlorella / Q000378:metabolism; D002848:Chromatography, DEAE-Cellulose; D000458:Cyanobacteria / Q000378:metabolism*; D004247:DNA / Q000032:analysis; D003837:Deoxy Sugars; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007553:Isotope Labeling; D008722:Methods; D008744:Methylamines; D009711:Nucleotides / Q000302:isolation & purification; D010727:Phosphoric Diester Hydrolases; D011687:Purines; D012313:RNA / Q000032:analysis; D013053:Spectrophotometry; D014461:Ultracentrifugation
D000596:Amino Acids; D002250:Carbon Radioisotopes; D003837:Deoxy Sugars; D008744:Methylamines; D009711:Nucleotides; D011687:Purines; D000249:Adenosine Monophosphate; D012313:RNA; D004247:DNA; D010727:Phosphoric Diester Hydrolases