Quantitative determination of lysolecithin and sphingomyelin in phospholipid mixtures by thin-layer chromatography as applied to the thymus of the fmfm mouse.
Lysolecithin and sphingomyelin may be readily separated from other phospholipids on thin-layer plates impregnated with silver nitrate using the solvent system chloroform-methanol-concentrated ammonium hydroxide 65:35:8. Quantitation is carried out by determining the phosphorus content of the developed bands. The minimum amount of phospholipid that can be quantitatively determined is that which contains 0.7 micro g of phosphorus. This method has been used for the determination of lysolecithin and sphingomyelin in the tissues of mice having foam-cell reticulosis.
D000818:Animals; D002784:Cholesterol / Q000032:analysis; D002855:Chromatography, Thin Layer; D005069:Evaluation Studies as Topic; D006801:Humans; D008244:Lysophosphatidylcholines / Q000302:isolation & purification*; D008722:Methods; D051379:Mice; D008815:Mice, Inbred Strains; D009542:Niemann-Pick Diseases / Q000378:metabolism; D010713:Phosphatidylcholines / Q000032:analysis; D010714:Phosphatidylethanolamines / Q000032:analysis; D010716:Phosphatidylinositols / Q000032:analysis; D012835:Silver Nitrate; D013109:Sphingomyelins / Q000302:isolation & purification*; D013950:Thymus Gland / Q000032:analysis*
D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010716:Phosphatidylinositols; D013109:Sphingomyelins; D012835:Silver Nitrate; D002784:Cholesterol