title: Chromatographic analyses of isoaccepting tRNAs from avian tumor viruses.
abstract: Low-molecular-weight RNA from transforming viruses (Rous sarcoma virus-Rous-associated virus 1, Schmidt-Ruppin strain of Rous sarcoma virus, and sarcoma-B(77)), from nontransforming viruses (Rous-associated virus 1 and sarcoma-NTB(77)), and from chicken liver, chicken embryo fibroblast, and Rous sarcoma virus-Rous-associated virus 1-transformed chicken embryo fibroblast was isolated and purified. To determine if there are modified, qualitatively or quantitatively different isoaccepting species of tRNA in these avian sarcoma viruses as compared with the cell of virus origin, chicken embryo fibroblast or normal chicken liver, methionyl-, arginyl-, and lysyl-tRNA (with high amino acid acceptance activity), and aspartyl- and glutamyl-tRNA from viral-trans-formed cells (with low viral amino acid acceptance activity) were co-chromatographed on reversed phase-5 chromatography columns, and elution profiles were compared. Although in each case the elution profile between a particular viral and host cell tRNA differed quantitatively, there was no qualitative difference in the profiles of corresponding tRNAs from either transforming or nontransforming viruses examined. Minor quantitative differences in the elution profiles might be a reflection of the metabolic state of the cells, since all evidence points to acceptor activity being of host rather than viral origin. Since, with the exception of selective packaging of methionyl-tRNA (IV) species by both transforming and nontransforming viruses, no selectivity was found for isoacceptor species of other tRNAs, it seems that such preferential packaging of methionyl-tRNA (IV) species has no bearing on the event of viral transformation.
mesh_terms: D001355:Alpharetrovirus / Q000032:analysis*; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D000818:Animals; D001120:Arginine / Q000378:metabolism; D001224:Aspartic Acid / Q000378:metabolism; D001358:Avian Sarcoma Viruses / Q000032:analysis*; D002250:Carbon Radioisotopes; D002471:Cell Transformation, Neoplastic; D002474:Cell-Free System; D002642:Chick Embryo; D002645:Chickens; D002845:Chromatography; D046508:Culture Techniques; D005347:Fibroblasts; D008099:Liver / Q000032:analysis / Q000201:enzymology; D008239:Lysine / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D012334:RNA, Neoplasm / Q000032:analysis; D012343:RNA, Transfer / Q000032:analysis* / Q000378:metabolism; D012367:RNA, Viral / Q000032:analysis*; D012526:Satellite Viruses / Q000032:analysis*; D014316:Tritium
chemical_list: D002250:Carbon Radioisotopes; D012334:RNA, Neoplasm; D012367:RNA, Viral; D014316:Tritium; D001224:Aspartic Acid; D012343:RNA, Transfer; D001120:Arginine; D008715:Methionine; D000604:Amino Acyl-tRNA Synthetases; D008239:Lysine