Protein kinase and phosphoproteins of vesicular stomatitis virus.
Protein kinases of similar but not identical activity were found associated with vesicular stomatitis (VS) virions grown in mouse L cells, primary chicken embryo (CE) cells, and BHK-21 cells, as well as being present in VS virions grown in HeLa and Aedes albopictus cells. The virion kinase preferentially phosphorylated the nucleocapsid NS protein in vitro and to a lesser extent the envelope M protein. Other virion proteins were phosphorylated in vitro only after drastic detergent treatment. Partial evidence that the virion kinase is of cellular origin was obtained by finding reduced enzyme activity in virions released from cells pretreated with actinomycin D and cycloheximide. Selective detergent and detergent-salt fractionation of VS virions revealed that the kinase activity was present in the envelope but not the spikes. The virion kinase activity in a Triton-salt-solubilized envelope fraction could be separated from M and G proteins and partially purified by phosphocellulose column chromatography. Virions released from L, CE, and BHK-21 cells infected in the presence of [(32)P]orthophosphate were labeled almost exclusively in the NS protein. Both soluble and nucleocapsid-associated NS phosphoprotein were present in cytoplasmic extracts of VS viral-infected L cells. The origin and function of the NS phosphoprotein remain to be elucidated.
D000330:Aedes; D000818:Animals; D002458:Cell Fractionation; D002460:Cell Line; D002642:Chick Embryo; D006224:Cricetinae; D046508:Culture Techniques; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006801:Humans; D007668:Kidney; D007739:L Cells; D051379:Mice; D010085:Oxidative Phosphorylation; D010750:Phosphoproteins / Q000096:biosynthesis*; D010761:Phosphorus Radioisotopes; D011494:Protein Kinases / Q000378:metabolism*; D012995:Solubility; D013501:Surface-Active Agents / Q000494:pharmacology; D014316:Tritium; D014721:Vesicular stomatitis Indiana virus / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis*
D010750:Phosphoproteins; D010761:Phosphorus Radioisotopes; D013501:Surface-Active Agents; D014764:Viral Proteins; D014316:Tritium; D003609:Dactinomycin; D003513:Cycloheximide; D011494:Protein Kinases