Intracellular cyclic AMP concentration responds specifically to growth regulation by serum.
Intracellular concentrations of cyclic AMP increase when cells are deprived of serum. Studies with the mouse fibroblast line 3T3, an SV40-transformed subline of 3T3, and six different revertant lines derived from this clone show that a marked increase in cyclic AMP occurs only when the serum concentration is reduced below the minimum necessary for growth of a given line. Conversely, density-dependent inhibition of growth is not accompanied by an increase in cyclic AMP concentration in any line.
D000818:Animals; D001769:Blood; D002455:Cell Division; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells / Q000378:metabolism*; D003260:Contact Inhibition; D003470:Culture Media; D000242:Cyclic AMP / Q000032:analysis / Q000378:metabolism*; D005347:Fibroblasts / Q000254:growth & development* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D013539:Simian virus 40
D003470:Culture Media; D000242:Cyclic AMP