Time of enterotoxin formation and release during sporulation of Clostridium perfringens type A.
Clostridium perfringens enterotoxin was detected intracellularly about 3 hr after the inoculation of vegetative cells into sporulation medium. The subsequent increase in intracellular enterotoxin concentration roughly paralleled but followed by 2.5 to 5 hr the increase in number of heat-resistant spores. The increase in biologically active toxin coincided with the increase in enterotoxin antigen. Enterotoxin was released from the sporangium with its lysis, concomitantly with the mature spore release.
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D003016:Clostridium perfringens / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media; D004768:Enterotoxins / Q000032:analysis / Q000096:biosynthesis*; D006168:Guinea Pigs; D006358:Hot Temperature; D005779:Immunodiffusion; D012882:Skin Tests; D013171:Spores, Bacterial / Q000254:growth & development; D013864:Thioglycolates; D013997:Time Factors
D000942:Antigens, Bacterial; D003470:Culture Media; D004768:Enterotoxins; D013864:Thioglycolates