Preferred site for initiation of RNA transcription by Escherichia coli RNA polymerase within the simian virus 40 DNA segment of the nondefective adenovirus-simian virus 40 hybrid viruses Ad2 + ND 1  and Ad2 + ND 3 .
The DNA of simian virus 40 (SV40) was transcribed into RNA by Escherichia coli RNA polymerase at 18 to 24 C after synchronization of the initiation of RNA synthesis. After a brief synthetic period the RNA product contained relatively large amounts of sequences derived from a limited segment of SV40 DNA. The source for this pulse-labeled RNA was found to be a portion of the segment of SV40 DNA included within the nondefective adenovirus (Ad)-SV40 hybrid viruses, Ad2(+)ND(1) and Ad2(+)ND(3). After synthesis with [gamma-(32)P] ATP, Ad2(+)ND(1) and Ad2(+)ND(3) DNA transcripts contained an initial sequence missing from Ad2 transcripts. This sequence was identified as an initiation sequence for polymerase transcription of the SV40 DNA. Thus, there is a preferred site for initiation of in vitro transcription on the segment of SV40 DNA common to the nondefective Ad2(+)ND(1) and Ad2(+)ND(3) hybrid viruses.
D000256:Adenoviridae / Q000032:analysis; D001345:Autoradiography; D001483:Base Sequence; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D006824:Hybridization, Genetic; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000032:analysis; D010759:Phosphorus Isotopes; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D012260:Ribonucleases; D013539:Simian virus 40 / Q000032:analysis; D014158:Transcription, Genetic
D004279:DNA, Viral; D009841:Oligonucleotides; D010759:Phosphorus Isotopes; D012367:RNA, Viral; D012321:DNA-Directed RNA Polymerases; D012260:Ribonucleases