Typing Herpesvirus hominis antibodies and isolates by inhibition of the indirect hemagglutination reaction.
Inhibition of the indirect hemagglutination reaction (IHA inhibition) was compared to several other methods for type-specific identification of Herpesvirus hominis (HVH) antibodies and isolates. The method appears to have the greatest value for typing antibodies for HVH type 1 and HVH type 2 in human sera; identification of antibody type was relatively simple and results were definitive. The IHA-inhibition test permitted serological diagnosis of HVH type 2 infection in three young adults with meningoencephalitis, thus extending the mounting evidence that nervous system involvement with this virus type is not limited to neonatal infections. II/I indexes of neutralizing or IHA antibody gave an accurate indication of the presence of HVH type 2 antibody in those sera containing type 2 antibody by IHA inhibition, but they indicated the presence of HVH type 2 antibody in one-half or more of the sera shown to contain only HVH type 1 antibody by IHA inhibition. For typing HVH isolates, the IHA-inhibition test gave results identical to those obtained by direct fluorescent-antibody staining using cross-absorbed conjugates, but the IHA-inhibition test was much more cumbersome and time-consuming to perform than was direct fluorescent-antibody staining. A microneutralization technique for virus typing also gave results identical to those obtained with direct fluorescent-antibody staining and IHA inhibition. However, typing HVH isolates by plaque size or the differential effect of incubation temperature was found to be less definitive and accurate.
D000328:Adult; D000914:Antibodies, Viral / Q000145:classification*; D003289:Convalescence; D003872:Dermatitis / Q000097:blood; D005069:Evaluation Studies as Topic; D005260:Female; D005455:Fluorescent Antibody Technique; D005831:Genital Diseases, Female / Q000097:blood; D005832:Genital Diseases, Male / Q000097:blood; D005892:Gingivitis, Necrotizing Ulcerative / Q000097:blood; D006385:Hemagglutination Inhibition Tests / Q000379:methods; D006386:Hemagglutination Tests; D006561:Herpes Simplex / Q000097:blood* / Q000175:diagnosis; D006801:Humans; D008297:Male; D008590:Meningoencephalitis / Q000097:blood; D009500:Neutralization Tests; D018139:Simplexvirus / Q000145:classification*; D010948:Viral Plaque Assay
D000914:Antibodies, Viral