The preparation of a modified GTP-sepharose derivative and its use in the purification of dihydroneopterin triphosphate synthetase, the first enzyme in folate biosynthesis.
D000645:Ammonium Sulfate; D001426:Bacterial Proteins / Q000032:analysis; D002247:Carbon Isotopes; D002845:Chromatography; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D005492:Folic Acid / Q000096:biosynthesis*; D006160:Guanosine Triphosphate; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000201:enzymology*; D008190:Lyases / Q000302:isolation & purification*; D008722:Methods; D010084:Oxidation-Reduction; D010504:Periodic Acid; D010710:Phosphates / Q000032:analysis; D010759:Phosphorus Isotopes; D011134:Polysaccharides; D011485:Protein Binding; D011622:Pterins; D012260:Ribonucleases
D001426:Bacterial Proteins; D002247:Carbon Isotopes; D010710:Phosphates; D010759:Phosphorus Isotopes; D011134:Polysaccharides; D011622:Pterins; D010504:Periodic Acid; D006160:Guanosine Triphosphate; D005492:Folic Acid; D012260:Ribonucleases; D008190:Lyases; D000645:Ammonium Sulfate