Conformations of diphosphopyridine coenzymes upon binding to dehydrogenases.
The binding of oxidized as well as reduced coenzyme to some dehydrogenases has been studied under different concentration ratios and temperatures by nuclear magnetic resonance spectroscopy. A significant difference in the spectral behavior between DPN(+) and DPNH upon binding is interpreted in terms of fast and slow on-off rates relative to the nuclear magnetic resonance time scale in the binding of these two coenzymes. Significant downfield shifts of DPN(+) were observed upon binding, comparable in magnitude to those expected upon opening (destacking) of the coenzymes in the case of chicken-muscle and lobster-tail lactate dehydrogenase (EC 1.1.1.27) and yeast alchol dehydrogenase (EC 1.1.1.1.). A preliminary survey of several other dehydrogenases is consistent with these findings. In the case of 3-phosphoglyceraldehyde dehydrogenase, there is a possibility that the coenzyme exists in the folded form.
D000429:Alcohol Oxidoreductases / Q000032:analysis*; D000818:Animals; D001665:Binding Sites; D002645:Chickens; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000032:analysis; D005993:Glycerolphosphate Dehydrogenase / Q000032:analysis; D006736:Horses; D007527:Isoenzymes; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008099:Liver / Q000201:enzymology; D009682:Magnetic Resonance Spectroscopy; D008291:Malate Dehydrogenase / Q000032:analysis; D008968:Molecular Conformation; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D009243:NAD / Q000032:analysis*; D008121:Nephropidae; D010084:Oxidation-Reduction; D011817:Rabbits; D013696:Temperature; D015003:Yeasts / Q000201:enzymology
D007527:Isoenzymes; D009243:NAD; D000429:Alcohol Oxidoreductases; D005993:Glycerolphosphate Dehydrogenase; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases