title: Reassortment of simian virus 40 DNA during serial undiluted passage.
abstract: Alterations occur in the supercoiled form of viral DNA after the serial undiluted passaging of simian virus (SV) 40. We have identified a portion of the viral genome which is amplified during this process. These SV40 DNA sequences represent about 30% of the viral genetic information and are present in a reiterated form in twisted circular molecules prepared from purified virions. In addition, reiterated and unique green monkey DNA sequences are incorporated into supercoiled viral DNA. The cellular DNA appears to be inserted at numerous locations in the DNA I molecules.
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D002478:Cells, Cultured; D002482:Cellulose; D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D004279:DNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D003851:Deoxyribonucleases; D004720:Endonucleases; D004926:Escherichia coli; D005374:Filtration; D006358:Hot Temperature; D007553:Isotope Labeling; D007668:Kidney; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D010761:Phosphorus Radioisotopes; D013539:Simian virus 40 / Q000032:analysis*; D014776:Virus Cultivation
chemical_list: D004279:DNA, Viral; D010761:Phosphorus Radioisotopes; D002482:Cellulose; D004247:DNA; D003851:Deoxyribonucleases; D004720:Endonucleases