title: Radioreceptor assay for prolactin and other lactogenic hormones.
abstract: A radioreceptor assay with a sensitivity of 5 nanograms per milliliter has been developed for mammalian and avian pituitary prolactin, placental lactogenic hormones, and humnan growth hormone, using a membrane receptor preparation isolated from rabbit mammary glands. Prolactin preparations inhibited the binding of [(125)I]prolactin to receptors in direct proportion to the biological potency of these preparations. Thus, the radioreceptor assay provides a convenient and simnple assay for hormones which have lactogenic activity.
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000032:analysis; D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D006063:Chorionic Gonadotropin / Q000032:analysis; D005260:Female; D005399:Fishes; D005640:Follicle Stimulating Hormone / Q000032:analysis; D013006:Growth Hormone / Q000032:analysis; D006168:Guinea Pigs; D000882:Haplorhini; D006801:Humans; D007456:Iodine Isotopes; D007986:Luteinizing Hormone / Q000032:analysis; D008321:Mammary Glands, Animal / Q000378:metabolism; D010928:Placental Lactogen / Q000032:analysis*; D011247:Pregnancy; D011388:Prolactin / Q000032:analysis* / Q000097:blood; D011817:Rabbits; D011869:Radioligand Assay; D051381:Rats; D011956:Receptors, Cell Surface; D012756:Sheep; D013972:Thyrotropin / Q000032:analysis; D014422:Turkeys
chemical_list: D006063:Chorionic Gonadotropin; D007456:Iodine Isotopes; D011956:Receptors, Cell Surface; D000324:Adrenocorticotropic Hormone; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013972:Thyrotropin; D013006:Growth Hormone; D010928:Placental Lactogen