Simple assay for monoacylglycerol hydrolase activity of rat adipose tissue.
A simple, sensitive, and specific assay for monoacylglycerol hydrolase activity of rat adipose tissue is described. Monoacyl[(3)H]glycerols with different chain lengths (8-18 carbon atoms) and different degrees of unsaturation in mixed micellar solution with different detergents can be used as substrates. The [(3)H]glycerol that is produced is isolated in a one-step liquid-liquid partition procedure. For routine purposes monooleoyl[(3)H]glycerol was found to be the most suitable substrate. A simple method for the chemical synthesis and purification of this substrate in high yield is given. The assay allows rapid serial sampling of enzymatic activity with a high reproducibility.
D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D002845:Chromatography; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004950:Esterases / Q000032:analysis*; D005069:Evaluation Studies as Topic; D005989:Glycerides; D005990:Glycerol; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008722:Methods; D008832:Microchemistry; D051381:Rats; D012822:Silicon Dioxide; D013329:Structure-Activity Relationship; D013501:Surface-Active Agents; D014316:Tritium
D005989:Glycerides; D013501:Surface-Active Agents; D014316:Tritium; D012822:Silicon Dioxide; D004950:Esterases; D005990:Glycerol