Quantitative assessment of hemadsorption by myxoviruses: anti-immunoglobulin G hemadsorption-inhibition test.
A quantitative hemadsorption-inhibition test was developed to estimate myxovirus serum antibodies within 24 h by determining the serum dilution inhibiting hemadsorption in 50% of the infected cells. The test depends on the interactions of virus-infected cell monolayers with antiviral serum and of the resultant complexes with antiimmunoglobulin G serum. The incorporation of species-specific anti-immunoglobulin G serum into the test significantly increased sensitivity.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002460:Cell Line; D002999:Clone Cells; D003228:Conjunctiva; D003429:Cross Reactions; D004912:Erythrocytes / Q000276:immunology; D005069:Evaluation Studies as Topic; D006168:Guinea Pigs / Q000276:immunology; D006382:Hemadsorption; D006383:Hemadsorption Inhibition Tests / Q000592:standards*; D006801:Humans; D007106:Immune Sera; D007074:Immunoglobulin G; D008722:Methods; D009975:Orthomyxoviridae / Q000254:growth & development / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D018108:Respirovirus / Q000254:growth & development / Q000276:immunology*; D013696:Temperature; D013997:Time Factors
D000914:Antibodies, Viral; D007106:Immune Sera; D007074:Immunoglobulin G