Methionine transfer ribonucleic acids of avian myeloblastosis virus.
Chick-embryo cells contain four isoaccepting species of methionine transfer RNA (I-IV). One species (I) is the initiator, tRNA(f) (Met), and the others (II, III, and IV) are the donors of internal methionyl residues (tRNA(m) (Met)). Over 85% of the tRNA(Met) in purified avian myeloblastosis virus consists of one tRNA(m) (Met) species, which resembles host-cell tRNA(Met) IV with respect to chromatographic properties on RPC-5, electrophoretic mobility of the terminal methionyl-oligonucleotide, and activity in cell-free protein synthesis in response to synthetic and natural messenger RNAs. Thus, tRNA(Met) IV of avian myeloblastosis virus is structurally indistinguishable from the corresponding host-cell species, and, although not involved in initiation of viral protein synthesis, is able to function in peptide-chain elongation.
D000818:Animals; D001345:Autoradiography; D001354:Avian Leukosis Virus / Q000032:analysis* / Q000302:isolation & purification; D001483:Base Sequence; D002642:Chick Embryo / Q000032:analysis* / Q000382:microbiology; D002845:Chromatography; D004586:Electrophoresis; D006868:Hydrolysis; D008715:Methionine / Q000378:metabolism; D051379:Mice; D009841:Oligonucleotides / Q000032:analysis; D010442:Peptide Chain Initiation, Translational; D012334:RNA, Neoplasm / Q000032:analysis; D012343:RNA, Transfer / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D012367:RNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D012260:Ribonucleases; D013462:Sulfur Radioisotopes; D014764:Viral Proteins / Q000096:biosynthesis
D009841:Oligonucleotides; D012334:RNA, Neoplasm; D012367:RNA, Viral; D013462:Sulfur Radioisotopes; D014764:Viral Proteins; D012343:RNA, Transfer; D008715:Methionine; D012260:Ribonucleases