Quantitation of endogenous C-type virion production in several murine cell lines.
Quantitation by enumeration of virion particles, measurement of absorbancy at 260 nm, and densitometry on sodium dodecyl sulfate-polyacrylamide gels has shown that mouse L-M cells yielded 7- to 10-fold less endogenous C-type virions than the parental lines, L or L929. The previously noted stimulation of L-M cell virion production by a concomitant 10% increase in fetal calf serum concentration (D. A. Kindig, R. Karp, and W. H. Kirsten, 1968), was not observed.
D000645:Ammonium Sulfate; D000818:Animals; D001798:Blood Proteins; D002417:Cattle; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D007739:L Cells / Q000382:microbiology*; D051379:Mice; D008854:Microscopy, Electron; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D012190:Retroviridae / Q000032:analysis / Q000254:growth & development* / Q000302:isolation & purification; D014617:Vacuoles / Q000382:microbiology; D014764:Viral Proteins / Q000032:analysis; D014779:Virus Replication
D001798:Blood Proteins; D003470:Culture Media; D010455:Peptides; D014764:Viral Proteins; D000645:Ammonium Sulfate