Polyoma viral DNA replicated as a nucleoprotein complex in close association with the host cell chromatin.
Polyoma viral DNA is shown to be replicated in close association with the mouse cell chromatin. Two virus-specific nucleoprotein complexes, designated complex A and B, can be dissociated from the isolated chromatin by gentle homogenization in 0.5 M NaCl. Complex A contains only replicating polyoma (Py) DNA whereas complex B contains only mature Py DNA I. The results show, furthermore, that complex A, containing viral DNA in different stages of replication, and complex B are both nucleoproteins with the same buoyant density. The data presently available suggest that newly synthesized stretches of Py DNA are immediately complexed with mouse cell histones and that complex B becomes the "core" of progeny Py virions. These results suggested that Py-induced replication of the mouse cell chromatin may be necessary to provide replicating Py DNA with histones.
D000818:Animals; D001345:Autoradiography; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D002843:Chromatin / Q000378:metabolism*; D004261:DNA Replication; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D007668:Kidney; D008722:Methods; D051379:Mice; D009693:Nucleic Acid Hybridization; D009698:Nucleoproteins / Q000096:biosynthesis* / Q000302:isolation & purification; D011120:Polyomavirus / Q000032:analysis / Q000254:growth & development / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014764:Viral Proteins / Q000096:biosynthesis* / Q000302:isolation & purification
D002250:Carbon Radioisotopes; D002843:Chromatin; D004279:DNA, Viral; D009698:Nucleoproteins; D014764:Viral Proteins; D014316:Tritium; D013936:Thymidine