title: Role of cyclic adenosine 3',5'-monophosphate in the in vivo expression of the galactose operon of Escherichia coli.
abstract: Studies of levels of galactokinase in Escherichia coli with mutations affecting synthesis of, or response to, cyclic adenosine 3',5'-monophosphate show that this nucleotide does not play a major role in expression of the galactose operon, causing at most a twofold stimulation. The discrepancy between our in vivo results and the marked stimulation by cyclic adenosine 3',5'-monophosphate in in vitro systems indicates that current cell-free systems lack a factor which allows efficient expression of the galactose operon even in the absence of cyclic adenosine 3',5'-monophosphate or of the binding protein for this nucleotide.
mesh_terms: D002247:Carbon Isotopes; D000242:Cyclic AMP / Q000378:metabolism*; D004790:Enzyme Induction; D004926:Escherichia coli / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism*; D005696:Galactosidases / Q000378:metabolism; D005827:Genetics, Microbial; D005838:Genotype; D005990:Glycerol; D008967:Molecular Biology; D009154:Mutation; D009876:Operon; D010770:Phosphotransferases / Q000378:metabolism; D013997:Time Factors
chemical_list: D002247:Carbon Isotopes; D000242:Cyclic AMP; D010770:Phosphotransferases; D005696:Galactosidases; D005990:Glycerol; D005690:Galactose