Virus-specific messenger RNA and nascent polypeptides in polyribosomes of cells replicating murine sarcoma-leukemia viruses.
We present evidence that virus-specific RNA is present in polyribosomes of transformed cells replicating the murine sarcoma-leukemia virus complex and that it serves as messenger RNA for the synthesis of viral-coded proteins. Both virus-specific RNA (detected by hybridization with the [(3)H]DNA product of the viral RNA-directed DNA polymerase) and nascent viral polypeptides (measured by precipitation with antiserum to purified virus) were found in membrane-bound and free polyribosomes. Membrane-bound polyribosomes contained a higher content of both virus-specific RNA and nascent viral polypeptides. From 60 to 70% of viral RNA sequences were released from polyribosomes with EDTA, consistent with a function as messenger RNA. Maximum amounts of both virus-specific RNA and nascent viral polypeptides were found in the polyribosome region sedimenting at about 350 S.
D000818:Animals; D002458:Cell Fractionation; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004279:DNA, Viral / Q000378:metabolism; D017988:Gammaretrovirus; D007118:Immunoassay; D051379:Mice; D009693:Nucleic Acid Hybridization; D010455:Peptides / Q000302:isolation & purification; D011132:Polyribosomes / Q000032:analysis*; D011233:Precipitin Tests; D012333:RNA, Messenger / Q000302:isolation & purification*; D012367:RNA, Viral / Q000302:isolation & purification* / Q000378:metabolism; D014316:Tritium; D014764:Viral Proteins / Q000302:isolation & purification*; D014779:Virus Replication
D004279:DNA, Viral; D010455:Peptides; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium