Cyclic adenosine 3',5'-monophosphate and morphogenesis in Mucor racemosus.
Yeastlike cells of Mucor racemosus grown under 100% CO(2) underwent morphogenesis to hyphae after exposure to air. The addition of dibutyryl cyclic adenosine monophosphate (dbcAMP) to yeastlike cultures inhibited this morphogenesis in media containing 2% glucose. The maintenance of uniformly spherical, budding cells required 1 mM dbcAMP in a defined medium containing Casamino Acids, and 3 mM dbcAMP in a medium containing yeast extract and peptone. At these concentrations, dbcAMP also induced yeastlike development in young aerobic hyphae grown in media containing 2% glucose. Removal of dbcAMP resulted in hyphal development. The endogenous cyclic AMP (cAMP) content of yeastlike cultures was measured after a shift from CO(2) to air. A fourfold decrease in intracellular cAMP preceded the appearance of hyphal germ tubes. These results indicate that cAMP plays a role in the control of morphogenesis in Mucor racemosus.
D000332:Aerobiosis; D003994:Bucladesine / Q000494:pharmacology; D002245:Carbon Dioxide; D002452:Cell Count; D003470:Culture Media; D000242:Cyclic AMP / Q000032:analysis / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D009024:Morphogenesis / Q000187:drug effects*; D009089:Mucor / Q000032:analysis / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D014316:Tritium
D003470:Culture Media; D014316:Tritium; D002245:Carbon Dioxide; D003994:Bucladesine; D000242:Cyclic AMP; D005947:Glucose