Nucleic acid synthesis in cytoplasm of Yaba monkey tumor virus-infected cells.
Yaba tumor virus progeny appeared in cynomolgus monkey kidney cells at 24 h postinfection and reached a plateau at 72 h in the first cycle of replication. Viral DNA synthesis was first detected at about 3 h and reached a peak in 18 h. Maximum coating of viral DNA in infected cells occurred at 4 days postinfection. Rapidly labeled RNA was synthesized in the cytoplasm of virus-infected cells. At 6 h postinfection 7 to 10S RNA was present; this species was present in greater amount at 12 h; at 24 h a truncated peak indicated the presence of 14 to 15S as well as 7 to 10S RNA. Hybridization data indicated that the largest peak of messenger RNA synthesis occurred at 11 to 13 h postinfection and a second, slightly smaller, peak occurred at 21 to 23 h.
D000818:Animals; D002250:Carbon Radioisotopes; D002458:Cell Fractionation; D002460:Cell Line; D003593:Cytoplasm / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis*; D000882:Haplorhini; D007668:Kidney; D008251:Macaca; D008851:Micropore Filters; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000096:biosynthesis*; D012335:RNA, Ribosomal / Q000096:biosynthesis; D013053:Spectrophotometry; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D014779:Virus Replication; D014999:Yaba monkey tumor virus / Q000254:growth & development* / Q000378:metabolism
D002250:Carbon Radioisotopes; D004279:DNA, Viral; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D014316:Tritium; D013936:Thymidine; D014529:Uridine