Structure of the mouse mammary tumor virus: polypeptides and glycoproteins.
The polypeptide and glycoprotein compositions of the mouse mammary tumor virus virion from primary monolayer cultures of BALB/cfC3H mouse mammary tumor cells were studied by polyacrylamide gel electrophoresis by using internal and external labeling and Coomassie blue and periodic acid Schiff (PAS) staining. Twelve polypeptides were reproducibly resolved by the combined methods. Five major polypeptides were demonstrable with estimated molecular weights of 52,000, 36,000, 28,000, 14,000, and 10,000. Seven minor polypeptides were also consistently detected and had estimated molecular weights of 70,000, 60,000, 46,000, 38,000, 30,000, 22,000, and 17,000. Carbohydrate was associated with five of these polypeptides as measured by PAS stain or [(3)H] glucosamine labeling, or both. These glycoproteins had estimated molecular weights of 70,000, 60,000, 52,000, 36,000 and 10,000. The majority of the PAS stain and glucosamine was found in the 52,000 and 36,000 dalton peaks.
D000596:Amino Acids; D000818:Animals; D002499:Centrifugation, Density Gradient; D046508:Culture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005944:Glucosamine; D006023:Glycoproteins / Q000032:analysis*; D005779:Immunodiffusion; D007457:Iodine Radioisotopes; D008325:Mammary Neoplasms, Experimental; D008324:Mammary Tumor Virus, Mouse / Q000032:analysis* / Q000254:growth & development; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis*; D014316:Tritium; D014764:Viral Proteins / Q000032:analysis*
D000596:Amino Acids; D006023:Glycoproteins; D007457:Iodine Radioisotopes; D010455:Peptides; D014764:Viral Proteins; D014316:Tritium; D005944:Glucosamine