Morphogenesis of poliovirus. II. Demonstration of a new intermediate, the proviron.
Poliovirus-infected cells contain a previously unrecognized particle which appears to be an intermediate in virion synthesis and therefore has been named proviron. It sediments at about 125S, contains the three procapsid proteins, VP-0, VP-1, and VP-3, and has 35S viral RNA. It is disrupted both by sodium dodecyl sulfate and EDTA but the RNA resists digestion by ribonuclease. Pulsechase experiments and studies employing the virus-specific inhibitor, guanidine, all indicate that the proviron is formed by combination of newly made RNA with the procapsid. Cleavage of VP-0 to form VP-2 and VP-4 follows formation of the provirion and would be the final step in poliovirus morphogenesis.
D002250:Carbon Radioisotopes; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D009698:Nucleoproteins / Q000302:isolation & purification* / Q000378:metabolism; D017955:Poliovirus / Q000254:growth & development*; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification*; D013997:Time Factors; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D014764:Viral Proteins / Q000302:isolation & purification* / Q000378:metabolism
D002250:Carbon Radioisotopes; D009698:Nucleoproteins; D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium; D007930:Leucine; D014529:Uridine