Kinetics of early changes in phosphate and uridine transport and cyclic AMP levels stimulated by serum in density-inhibited 3T3 cells.
A kinetic study of the early changes in uridine- and phosphate-transport rates and 3':5'-cyclic AMP levels induced by the addition of serum to quiescent 3T3 cells revealed that the increase in phosphate uptake and the decrease in the intracellular concentration of 3':5'-cyclic AMP immediately follow the addition of serum. In contrast, the increase in uridine transport occurred after a lag of several minutes and was clearly preceded by the other changes. The activation of phosphate transport involved an increase in V(max), while a more complex pattern was observed for uridine transport. A possible role of phosphate in connection with the other early events was also studied. The addition of phosphate to quiescent cells produced a time-dependent decrease in 3':5'-cyclic AMP levels and this ion was required for full activation of uridine transport by serum. The results show that the increase in phosphate transport is a primary event in the reinitiation of growth and suggest that the increase in phosphate transport may be connected with subsequent metabolic steps.
D000818:Animals; D001769:Blood; D002460:Cell Line; D003260:Contact Inhibition; D000242:Cyclic AMP / Q000378:metabolism*; D005347:Fibroblasts; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D010710:Phosphates / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D014316:Tritium; D014529:Uridine / Q000378:metabolism*
D010710:Phosphates; D010761:Phosphorus Radioisotopes; D014316:Tritium; D000242:Cyclic AMP; D014529:Uridine