Positive control of transformed phenotype in hybrids between SV40-transformed and normal human cells.
Somatic cell hybrids have been obtained between SV40-transformed Lesch-Nyhan fibroblasts, which are deficient in hypoxanthine-guanine phosphoribosyltransferase (HGPRT) and display glucose-6-phosphate dehydrogenase A (G6PD-A) activity, and late-passage HGPRT-positive W138 human embryo fibroblasts, which display G6PD-B activity. The human-human hybrid clones, which display G6PD-A and G6PD-B and heteropolymers of the two enzyme forms, have the same growth characteristic as the SV40-transformed parental cells and behave as continuous cell lines. The SV40 tumor antigen, the gene for which has been assigned to human chromosome 7, is present in all clones examined.
D000951:Antigens, Neoplasm / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D002455:Cell Division; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002906:Chromosomes, Human, 6-12 and X; D002999:Clone Cells; D003260:Contact Inhibition; D005347:Fibroblasts; D005796:Genes; D005838:Genotype; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology / Q000276:immunology*; D007621:Karyotyping; D007926:Lesch-Nyhan Syndrome; D008168:Lung / Q000196:embryology; D010430:Pentosyltransferases / Q000378:metabolism; D010641:Phenotype; D011123:Polyploidy; D012730:Sex Chromosomes; D013539:Simian virus 40 / Q000276:immunology*
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D005954:Glucosephosphate Dehydrogenase; D010430:Pentosyltransferases