Filtration methods for recovering enteroviruses from foods.
A set of simple, rapid filtration methods has been developed for recovering enteroviruses from foods. The methods employ quite inexpensive equipment, and the resulting extract might be concentrated in any of several ways. Three model enteroviruses were apparently recovered with at least 80% efficiency when inoculated, at levels ranging from thousands to one plaque-forming unit per 20-g sample, into eleven foods representing six major food groups.
D000818:Animals; D046508:Culture Techniques; D003588:Cytopathogenic Effect, Viral; D004770:Enterovirus / Q000302:isolation & purification*; D029822:Enterovirus B, Human / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D005374:Filtration; D005516:Food Microbiology; D000882:Haplorhini; D007668:Kidney; D008251:Macaca; D008722:Methods; D017955:Poliovirus / Q000302:isolation & purification*; D013242:Sterilization; D010948:Viral Plaque Assay; D014776:Virus Cultivation