Mutagenesis and enrichment of auxotrophs in Penicillium chrysogenum.
An efficient method for isolation of auxotrophs of Penicillium chyrysogenum involving mutagenesis with ethyl methanesulfonate followed by enrichment with sodium pentachlorophenate was developed. The auxotroph frequencies obtained were 30 to 40%.
D000476:Alkanesulfonates; D003470:Culture Media; D008239:Lysine / Q000378:metabolism; D008722:Methods; D008851:Micropore Filters; D009153:Mutagens; D009154:Mutation; D010407:Penicillium / Q000302:isolation & purification*; D010408:Penicillium chrysogenum / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D010416:Pentachlorophenol; D013172:Spores, Fungal / Q000254:growth & development
D000476:Alkanesulfonates; D003470:Culture Media; D009153:Mutagens; D010416:Pentachlorophenol; D008239:Lysine