title: Temperature-sensitive mutations in Drosophila melanogaster. XVII. Heat- and cold-sensitive lethals on chromosome 3.
abstract: Ethyl methanesulfonate-treated third chromosome of Drosophila melanogaster were tested for the presence of dominant and recessive temperature-sensitive lethal mutations at 17 degrees , 22 degrees and 29 degrees C. Out of 1,176 chromosomes tested, no dominant ts lethals, 21 heat-sensitive, 22 cold-sensitive and 10 heat-cold-sensitive lethals were recovered. Heat-cold sensitivity was produced by a single mutation in all cases. Sixty-two percent of the ts lethals were fertile as homozygotes in both sexes. Surprisingly, 88% of the ts lethals mapped between st and Sb, a region straddling the centromere and estimated to comprise 12.9% of the genetic length and 55% of the physical length of chromosome 3. All but one of the heat- and cold-sensitive lethals complemented with each other at their respective restrictive temperatures.
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D002875:Chromosomes / Q000187:drug effects; D003080:Cold Temperature; D003433:Crosses, Genetic; D004331:Drosophila melanogaster; D005260:Female; D005799:Genes, Dominant; D005804:Genes, Lethal; D005808:Genes, Recessive; D005816:Genetic Complementation Test; D006579:Heterozygote; D006358:Hot Temperature; D008297:Male; D008698:Mesylates / Q000494:pharmacology; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation / Q000187:drug effects; D013696:Temperature
chemical_list: D008698:Mesylates; D009153:Mutagens