title: Dexamethasone stimulation of murine mammary tumor virus expression: a tissue culture source of virus.
abstract: In mouse cell lines derived from mammary adenocarcinomnas, the synthetic steroid dexamnethasone stimulates production of murine mammary tumor virus. Viral RNA and antigens are increased as much as 20-fold, and culture fluid supernatants from steroid-treated cells contain type B particles with reverse transcriptase. These cells provide a possible tissue culture source of this virus and a model system for studying the mechanism of action of corticosteroids and the regulation of transcription of integrated viral DNA.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D002999:Clone Cells; D004279:DNA, Viral; D003907:Dexamethasone / Q000494:pharmacology*; D005260:Female; D005455:Fluorescent Antibody Technique; D008325:Mammary Neoplasms, Experimental / Q000382:microbiology*; D008324:Mammary Tumor Virus, Mouse / Q000254:growth & development* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008854:Microscopy, Electron; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis; D013268:Stimulation, Chemical; D014158:Transcription, Genetic / Q000187:drug effects; D014764:Viral Proteins / Q000096:biosynthesis; D014776:Virus Cultivation; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral; D012367:RNA, Viral; D014764:Viral Proteins; D003907:Dexamethasone