Characterization of two simian virus 40-specific RNA molecules from infected BS-C-1 cells.
Two discrete simian virus 40 (SV40) RNA species sedimenting at 19 and 16S, respectively, that are present in infected BS-C-1 cells were characterized with respect to the base composition and the ribonuclease T1 fingerprints. The base composition of the 19S SV40 RNA was found to be cytidylic acid (C), 23.0; adenylic acid (A), 28.3; guanylic acid (G), 23.9; and uridylic acid (U), 24.8; that of the 16S SV40 RNA was C, 19.3; A, 34.0; G, 22.0; and U, 24.7 mol%. Analysis of the ribonuclease T1 fingerprints indicated a difference in the base sequence of the 19 and 16S SV40 RNA. The presence of long sequences of adenylic acid residues (poly A) in these viral RNAs was confirmed.
D000241:Adenosine / Q000032:analysis; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D003597:Cytosine Nucleotides / Q000032:analysis; D004590:Electrophoresis, Paper; D004591:Electrophoresis, Polyacrylamide Gel; D006150:Guanine Nucleotides / Q000032:analysis; D000882:Haplorhini; D007668:Kidney; D009693:Nucleic Acid Hybridization; D009711:Nucleotides / Q000032:analysis; D010759:Phosphorus Isotopes; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D012260:Ribonucleases; D013539:Simian virus 40 / Q000032:analysis* / Q000254:growth & development; D014316:Tritium; D014500:Uracil Nucleotides / Q000032:analysis
D003597:Cytosine Nucleotides; D006150:Guanine Nucleotides; D009711:Nucleotides; D010759:Phosphorus Isotopes; D012367:RNA, Viral; D014500:Uracil Nucleotides; D014316:Tritium; D012260:Ribonucleases; D000241:Adenosine