Pyridoxal phosphate-sensitized photoinactivation of glutamate decarboxylase from Clostridium perfringens.
1. l-Glutamate decarboxylase (EC 4.1.1.15) from Clostridium perfringens was inactivated by exposure to visible light at pH6.2. 2. Inactivation does not occur at pH4.6 or in the absence of bound pyridoxal phosphate. 3. On prolonged photo-oxidation six histidine residues per molecule of enzyme were destroyed. 4. The loss of six cysteine residues per molecule occurred both in irradiated samples and in controls oxygenated in the dark. 5. This dark-oxidation of cysteine residues is apparently required before the photo-oxidation process. 6. The absorbance, fluorescence and circular-dichroism properties of the enzyme as well as its elution volume during Sephadex gel-filtration were unaffected by prolonged irradiation. 7. However, an apparently homogeneous product of photo-oxidation could be separated from the control enzyme by ion-exchange chromatography. 8. The K(m) for l-glutamate was unchanged in an irradiated sample retaining 22% of control activity. 9. These data and the catalytic role of imidazole residues at the active sites of amino acid decarboxylases are discussed.
D000596:Amino Acids / Q000032:analysis; D002262:Carboxy-Lyases / Q000032:analysis; D002852:Chromatography, Ion Exchange; D002942:Circular Dichroism; D003016:Clostridium perfringens / Q000201:enzymology*; D003545:Cysteine; D005971:Glutamates; D006639:Histidine; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010084:Oxidation-Reduction; D010100:Oxygen; D010777:Photochemistry; D011732:Pyridoxal Phosphate; D013050:Spectrometry, Fluorescence; D013997:Time Factors
D000596:Amino Acids; D005971:Glutamates; D006639:Histidine; D011732:Pyridoxal Phosphate; D002262:Carboxy-Lyases; D003545:Cysteine; D010100:Oxygen