Adenosine triphosphate-linked control of Pseudomonas aeruginosa glucose-6-phosphate dehydrogenase.
Extracts of Pseudomonas aeruginosa (ATCC 7700) cells grown on glucose, gluconate, or glycerol had enzyme activities related to the Entner-Doudoroff pathway. These activities were present in no more than trace amounts when the bacteria were grown on succinate. Fructose-1,6-diphosphate aldolase could not be detected in extracts of the bacteria grown on any of the above carbon sources. Therefore, it appears that P. aeruginosa degrades glucose via an inducible Entner-Doudoroff pathway. The apparent absence of fructose-1,6-diphosphate aldolase in cells growing on succinate suggests that the bacteria can form hexose and pentose phosphates from succinate by an alternate route. d-Glucose-6-phosphate dehydrogenase, a branch-point enzyme of the Entner-Doudoroff pathway, was purified 50-fold from glucose-grown cells. Its molecular weight, estimated by sucrose density gradient centrifugation, was found to be approximately 190,000. The enzyme was strongly inhibited by adenosine triphosphate, guanosine triphosphate, and deoxyguanosine triphosphate, which decreased the apparent binding of glucose-6-phosphate to the enzyme. It is suggested that adenine nucleotide-linked control of glucose-6-phosphate dehydrogenase may regulate the overall catabolism of hexose phosphates and prevent their wasteful degradation under certain conditions requiring gluconeogenesis.
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000446:Aldehyde-Lyases / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D004790:Enzyme Induction; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D005942:Gluconates / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D005954:Glucosephosphate Dehydrogenase / Q000096:biosynthesis*; D005990:Glycerol / Q000494:pharmacology; D006150:Guanine Nucleotides / Q000494:pharmacology; D006836:Hydro-Lyases / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000378:metabolism; D013386:Succinates / Q000494:pharmacology
D005942:Gluconates; D006150:Guanine Nucleotides; D013386:Succinates; D009243:NAD; D009249:NADP; D000255:Adenosine Triphosphate; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D010770:Phosphotransferases; D000446:Aldehyde-Lyases; D005634:Fructose-Bisphosphate Aldolase; D006836:Hydro-Lyases; D005947:Glucose; D005990:Glycerol