Studies on energy-linked reactions. Energy-linked transhydrogenase reaction in Escherichia coli.
1. Small particles from Escherichia coli catalyse an ATP-dependent reduction of NADP(+) by NADH. 2. The reaction was stimulated by Mg(2+) and only ATP and ITP could serve as energy donors. 3. Studies of the stoicheiometry of the reaction indicate that 1-2mol of ATP are utilized/mol of NADH produced. 4. The reaction is inhibited by uncoupling agents such as 2,4-dinitrophenol (200mum) and tetrachlorotrifluorobenzimidazole (2mum) but oligomycin does not inhibit. 5. The reaction is inhibited by relatively high concentrations (200mum) of piericidin A.
D000255:Adenosine Triphosphate / Q000378:metabolism; D001562:Benzimidazoles / Q000494:pharmacology; D003864:Depression, Chemical; D004140:Dinitrophenols / Q000494:pharmacology; D004579:Electron Transport; D004926:Escherichia coli / Q000201:enzymology*; D007292:Inosine Nucleotides / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D009243:NAD / Q000096:biosynthesis / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D009840:Oligomycins / Q000494:pharmacology; D010088:Oxidoreductases / Q000378:metabolism*; D011725:Pyridines / Q000494:pharmacology; D013268:Stimulation, Chemical
D001562:Benzimidazoles; D004140:Dinitrophenols; D007292:Inosine Nucleotides; D009840:Oligomycins; D011725:Pyridines; D009243:NAD; D009249:NADP; D000255:Adenosine Triphosphate; D010088:Oxidoreductases; D008274:Magnesium