Growth of foot-and-mouth disease virus in the different layers of bovine omasum in suspended cultures.
When suspended cultures of bovine omasum were cultured without agitation, the epithelium soon degenerated and foot-and-mouth disease virus multiplied mainly in the corium cells. Five days of preincubation were needed to reach a population of corium cells that could yield virus at a titer of 10(6.70) to 10(6.95) mean tissue culture infective doses per ml. The virus was freely released from the cells into the medium only when the degenerated epithelium was removed from the subepithelial tissue prior to virus inoculation. In agitated cultures, the viability of the epithelium was retained, the virus multiplied in all the layers of the epithelium and was freely released into the medium, and a virus titer of 10(6.95) mean tissue culture infective doses per ml was obtained without preincubation. The omasal laminae could be separated along the line of apposition of the two mucous membranes of the organ. The virus yield from these thin separated membranes was 0.5 to 1.0 log higher than that obtained from nonseparated laminae.
D000818:Animals; D005537:Aphthovirus / Q000254:growth & development* / Q000302:isolation & purification; D002417:Cattle; D046508:Culture Techniques; D004848:Epithelium / Q000382:microbiology; D009092:Mucous Membrane / Q000382:microbiology; D009851:Omasum / Q000166:cytology / Q000382:microbiology*; D014776:Virus Cultivation