Release of newly synthesized dopamine from dopamine-containing terminals in the striatum of the rat.
The extraneuronal spontaneous efflux of (3)H-dopamine synthesized endogenously in dopamine terminals has been demonstrated in vitro, by collecting the (3)H amine in superfusates of isolated striatum of the rat previously incubated with (3)H-tyrosine. The (3)H-dopamine newly synthesized appears to be preferentially released. Furthermore, amphetamine and a monoamine oxidase inhibitor (Carton) markedly increased the release of (3)H-dopamine. This effect was also observed with substances normally contained in some striatal neurons: acetylcholine and 5-hydroxytryptamine.
D000109:Acetylcholine / Q000494:pharmacology; D000661:Amphetamine / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D002421:Caudate Nucleus / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D004298:Dopamine / Q000096:biosynthesis / Q000378:metabolism*; D006834:Hydrazines / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D008722:Methods; D051381:Rats; D012701:Serotonin / Q000494:pharmacology; D014316:Tritium; D014443:Tyrosine / Q000378:metabolism
D006834:Hydrazines; D014316:Tritium; D012701:Serotonin; D014443:Tyrosine; D000661:Amphetamine; D000109:Acetylcholine; D004298:Dopamine