title: Events occurring near the time of synapsis during transformation in Diplococcus pneumoniae.
abstract: A marker-specific and strongly temperature-dependent reaction was observed to occur at a time during transformation in Diplococcus pneumoniae after the donor deoxyribonucleic acid (DNA) had acquired single-strand properties and immediately preceding the integration of these strands into the recipient chromosome. Operationally, it was observed as the prevention of an intracellular inactivation process, also described in this paper, which is specific for low molecular weight or for damaged DNA, and which occurs if the recipient cells are held at suboptimal temperatures after the DNA has entered. Brief exposure of the cells to a higher temperature stabilized the DNA against this inactivation, in a two step process. It is the first step which has a strong temperature dependence (DeltaHdouble dagger = 70 kcal/mole, DeltaSdouble dagger = 160 entropy units), is marker specific, and which appears to be reversible. The second step is much less temperature-dependent and overlaps in time the start of integration. The enthalpy and entropy of activation are both consistent with those needed to open a loop of six to eight base pairs in a DNA duplex. It is suggested that these observations may reflect, and provide an assay for, the kinetics of synapsis, which on this model is limited in rate by the appearance of unpaired regions on the recipient duplex.
mesh_terms: D002876:Chromosomes, Bacterial; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis / Q000528:radiation effects; D003851:Deoxyribonucleases; D008540:Meiosis; D008956:Models, Chemical; D008970:Molecular Weight; D011830:Radiation Effects; D011995:Recombination, Genetic; D013296:Streptococcus pneumoniae / Q000254:growth & development; D013696:Temperature; D013997:Time Factors; D014170:Transformation, Genetic; D014461:Ultracentrifugation; D014466:Ultraviolet Rays
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D003851:Deoxyribonucleases