In vivo and in vitro manifestations of adrenergic blockade in Bordetella pertussis-vaccinated mice.
The in vivo metabolic events which follow the administration of epinephrine, norepinephrine, or isoproterenol were examined in normal, Bordetella pertussis-vaccinated, and alpha and beta adrenergically blocked mice. The normal hyperglycemic response to epinephrine was suppressed in all experimental groups. The pertussis-sensitized and beta-blocked animals produced similar split patterns of altered response not duplicated by the alpha-blocking compounds. Those catecholamines that normally increase free fatty acids and lactic acid in the circulation failed to do so in the pertussis-sensitized and beta-blocked animals; the inhibition of free fatty acid mobilization was also demonstrated with adipose tissue incubated in vitro. An extract of the pertussis organism added to incubation media prevented the catecholamine-induced free fatty acid response. The epinephrine-stabilizing effect of bovine serum albumin (Cohn-fraction V) was observed. The results of these studies further emphasize a correlation between pertussis-sensitized and beta-adrenergically blocked mice.
D000818:Animals; D001341:Autonomic Nervous System / Q000187:drug effects*; D003983:Dibenzylchlorethamine / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology*; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D005260:Female; D007545:Isoproterenol / Q000494:pharmacology*; D007773:Lactates / Q000378:metabolism; D051379:Mice; D009638:Norepinephrine / Q000494:pharmacology*; D010567:Pertussis Vaccine / Q000494:pharmacology*; D010646:Phentolamine / Q000494:pharmacology*; D011433:Propranolol / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D005230:Fatty Acids, Nonesterified; D007773:Lactates; D010567:Pertussis Vaccine; D012710:Serum Albumin, Bovine; D003983:Dibenzylchlorethamine; D011433:Propranolol; D007545:Isoproterenol; D009638:Norepinephrine; D004837:Epinephrine; D010646:Phentolamine