Purification of pneumolysin.
A procedure for the purification of pneumolysin has been developed. Its use results in a 96-fold increase in specific activity from the original crude pneumococcal extract to the final purified product. The purified material is heat-labile and is inactivated by trypsin and cholesterol; inactivation by p-hydroxymercuribenzoate can be reversed by mercaptoethanol. Intravenous administration of the material to rabbits results in crenation, increased osmotic fragility, and intravascular lysis of the erythrocytes of the animal.
D000645:Ammonium Sulfate; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D002784:Cholesterol / Q000494:pharmacology; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000187:drug effects; D006460:Hemolysin Proteins / Q000008:administration & dosage / Q000032:analysis / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D006902:Hydroxymercuribenzoates / Q000494:pharmacology; D007275:Injections, Intravenous; D008623:Mercaptoethanol / Q000494:pharmacology; D009996:Osmotic Fragility / Q000187:drug effects; D011817:Rabbits / Q000276:immunology; D013053:Spectrophotometry; D013296:Streptococcus pneumoniae / Q000032:analysis*; D014357:Trypsin / Q000494:pharmacology
D001426:Bacterial Proteins; D006460:Hemolysin Proteins; D006902:Hydroxymercuribenzoates; D008623:Mercaptoethanol; D002784:Cholesterol; D014357:Trypsin; D000645:Ammonium Sulfate