Use of erythrocytes sensitized with purified pneumococcal polysaccharides for the assay of antibody and antibody-producing cells.
A method was described for the sensitization of erythrocytes with purified type-specific pneumococcal polysaccharide antigens using chromium chloride as a coupling agent. Erythrocytes so sensitized can be used in routine passive hemagglutination and hemolysis tests as well as in the technique of localized hemolysis-in-gel for the detection of specific antibody and specific antibody-producing cells, respectively.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000917:Antibody Formation; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D006386:Hemagglutination Tests; D006461:Hemolysis; D051379:Mice; D011135:Polysaccharides, Bacterial; D013154:Spleen / Q000276:immunology; D013296:Streptococcus pneumoniae / Q000276:immunology*
D000906:Antibodies; D011135:Polysaccharides, Bacterial