title: Phenylalanine and tyrosine biosynthesis in Escherichia coli K-12: mutants derepressed for 3-deoxy-D-arabinoheptulosonic acid 7-phosphate synthetase (phe), 3-deoxy-D-arabinoheptulosonic acid 7-phosphate synthetase (tyr), chorismate mutase T-prephenate dehydrogenase, and transaminase A.
abstract: Mutant strains of Escherichia coli have been isolated in which the synthesis of 3-deoxy-d-arabinoheptulosonic acid 7-phosphate (DAHP) synthetase (phe) is derepressed, in addition to those enzymes of tyrosine biosynthesis previously shown to be controlled by the gene tyrR. The major enzyme of the terminal pathway of phenylalanine biosynthesis chorismate mutase-prephenate dehydratase is not derepressed in these strains. Genetic analysis of the mutants shows that the mutation or mutations causing derepression map close to previously reported tyrR mutations. A study of one of the mutations has shown it to be recessive to the wild-type allele in a diploid strain. It is proposed that the tyrR gene product is involved in the regulation of the synthesis of DAHP synthetase (phe) as well as the synthesis of DAHP synthetase (tyr), chorismate mutase-prephenate dehydrogenase, and transaminase A.
mesh_terms: D000446:Aldehyde-Lyases / Q000378:metabolism*; D000483:Alleles; D002474:Cell-Free System; D002874:Chromosome Mapping; D003470:Culture Media; D003509:Cyclohexanecarboxylic Acids; D004352:Drug Resistance, Microbial; D004794:Enzyme Repression; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D005796:Genes; D005827:Genetics, Microbial; D006539:Heptoses; D006836:Hydro-Lyases / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism; D009154:Mutation; D010649:Phenylalanine / Q000096:biosynthesis* / Q000494:pharmacology; D011995:Recombination, Genetic; D013780:Tetroses; D014161:Transduction, Genetic; D014443:Tyrosine / Q000096:biosynthesis*; D014444:Tyrosine Transaminase / Q000378:metabolism*
chemical_list: D003470:Culture Media; D003509:Cyclohexanecarboxylic Acids; D006539:Heptoses; D007527:Isoenzymes; D013780:Tetroses; D014443:Tyrosine; D010649:Phenylalanine; D014444:Tyrosine Transaminase; D000446:Aldehyde-Lyases; D006836:Hydro-Lyases