Carrier function in anti-hapten antibody responses. IV. Experimental conditions for the induction of hapten-specific tolerance or for the stimulation of anti-hapten anamnestic responses by "nonimmunogenic" hapten-polypeptide conjugates.
Administration of nonimmunogenic 2,4-dinitrophenyl (DNP) conjugates of copolymers of D or L-glutamic acid and lysine (GL) induces hapten-specific tolerance in nonimmune and DNP-ovalbumin-primed strain 13 guinea pigs. This tolerant state is evidenced by depressed anti-DNP antibody synthesis in response to challenge with DNP-ovalbumin and by a diminished frequency of DNP-specific antigen-binding cells and of anti-DNP antibody-secreting cells. Such a nonimmunogenic compound (DNP-D-GL) will nevertheless elicit a DNP-specific anamnestic antibody response when administered at an appropriate time to DNP-ovalbumin-primed guinea pigs undergoing a graft-versus-host reaction. These experiments are discussed in terms of a two-cell theory of stimulation of antibody responses.
D000818:Animals; D000906:Antibodies; D000917:Antibody Formation; D000918:Antibody Specificity; D000921:Antibody-Producing Cells; D001345:Autoradiography; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D004140:Dinitrophenols; D005971:Glutamates; D006087:Graft vs Host Reaction; D006168:Guinea Pigs; D006241:Haptens; D007108:Immune Tolerance; D007111:Immunity, Cellular; D007116:Immunization, Passive; D007456:Iodine Isotopes; D008239:Lysine; D010047:Ovalbumin; D013950:Thymus Gland / Q000276:immunology
D000906:Antibodies; D004140:Dinitrophenols; D005971:Glutamates; D006241:Haptens; D007456:Iodine Isotopes; D010047:Ovalbumin; D008239:Lysine