Mutant strains of Escherichia coli K12 that use D-amino acids.
A series of mutations has been isolated that confer upon amino-acid auxotrophs of Escherichia coli K-12 the ability to grow when fed various D-amino acids. Several distinct systems, mediating cellular use of the D-isomers of leucine, histidine, phenylalanine, tyrosine, tryptophan, isoleucine, and valine, can be mutationally activated. Mutations leading to D-tryptophan use (dadR) all map near purB. They result in high activities of an enzyme that deaminates D-amino acids. Neither the enzymes of the tryptophan biosynthetic pathway nor tryptophanase (EC 4.2.1.e) are involved in D-tryptophan utilization.
D000596:Amino Acids / Q000378:metabolism*; D001120:Arginine / Q000378:metabolism; D002874:Chromosome Mapping; D003090:Coliphages; D003605:D-Amino-Acid Oxidase / Q000032:analysis; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D008040:Genetic Linkage; D005838:Genotype; D006639:Histidine / Q000378:metabolism; D007532:Isoleucine / Q000378:metabolism; D007536:Isomerism; D007930:Leucine / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D009154:Mutation; D010649:Phenylalanine / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D012694:Serine / Q000378:metabolism; D013912:Threonine / Q000378:metabolism; D014161:Transduction, Genetic; D014364:Tryptophan / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism; D014633:Valine / Q000378:metabolism
D000596:Amino Acids; D007532:Isoleucine; D013912:Threonine; D014443:Tyrosine; D012694:Serine; D010649:Phenylalanine; D006639:Histidine; D014364:Tryptophan; D001120:Arginine; D011392:Proline; D003605:D-Amino-Acid Oxidase; D007930:Leucine; D014633:Valine; D008239:Lysine