Partial purification by affinity chromatography of tyrosine aminotransferase-synthesizing ribosomes from hepatoma tissue culture cells.
This report describes a cell-free protein-synthesizing system derived from hepatoma tissue culture cells in which the synthesis of tyrosine aminotransferase is enhanced in relation to total protein synthesis. The enhancement was achieved by using affinity chromatography to select a specific fraction of ribosomes in which the proportion of tyrosine aminotransferase synthesis is higher than it is in unfractionated ribosomes.
D000327:Adsorption; D000818:Animals; D002247:Carbon Isotopes; D006528:Carcinoma, Hepatocellular / Q000201:enzymology*; D002474:Cell-Free System; D002845:Chromatography; D046508:Culture Techniques; D007118:Immunoassay; D008113:Liver Neoplasms; D009363:Neoplasm Proteins / Q000096:biosynthesis; D010847:Picolines; D011134:Polysaccharides; D011233:Precipitin Tests; D012270:Ribosomes / Q000201:enzymology*; D014316:Tritium; D014444:Tyrosine Transaminase / Q000096:biosynthesis*
D002247:Carbon Isotopes; D009363:Neoplasm Proteins; D010847:Picolines; D011134:Polysaccharides; D014316:Tritium; D014444:Tyrosine Transaminase