Catabolism of [4-14C]testosterone by subcellular fractions of human prostate.
1. Incubation conditions were established in experiments with human-prostate homogenates for almost complete conversion of [4-(14)C]testosterone into at least ten transformation products. 2. Whole homogenates of tissue with benign hypertrophy were shown to contain 3alpha-, 3beta- and 17beta-hydroxy steroid dehydrogenases, Delta(4)-3-oxo steroid 5alpha- and 5beta- reductases and unidentified hydroxylases. 3. Most of the 17beta-hydroxy steroid-dehydrogenase activity was located in the mitochondria, which showed little other activity. 4. The 3alpha- and 3beta-hydroxy steroid dehydrogenases and the 5beta-reductase were located in the high-speed supernatant and required supplementation with NADPH for activity. 5. The 5alpha-reductase was located in both microsomal and high-speed supernatant fractions and also required supplementation with NADPH.
D002247:Carbon Isotopes; D002849:Chromatography, Gas; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D006801:Humans; D006913:Hydroxysteroid Dehydrogenases / Q000378:metabolism; D008297:Male; D008861:Microsomes / Q000201:enzymology*; D008928:Mitochondria / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000378:metabolism; D009249:NADP; D010088:Oxidoreductases / Q000378:metabolism; D011467:Prostate / Q000201:enzymology*; D013739:Testosterone / Q000378:metabolism*
D002247:Carbon Isotopes; D013739:Testosterone; D009249:NADP; D006899:Mixed Function Oxygenases; D010088:Oxidoreductases; D006913:Hydroxysteroid Dehydrogenases