The anamnestic antibody response to type 3 specific pneumococcal polysaccharide.
Rabbits immunized with killed type III pneumococci respond to anamnestic challenge by type specific polysaccharide (S III) with the synthesis of anti-S III antibody if a long interval is allowed to elapse between primary and secondary immunization. A study of the anti-S III antibody produced early and late in the immune response revealed no change in molecular class, banding pattern of dissociated light chains, or S III binding characteristics as measured under equilibrium conditions or by study of dissociation kinetics utilizing radioiodinated p-OH-benzyl-S III. Sequential booster injections of S III into rabbits primarily immunized with whole organisms 8 or 9 months earlier led to a progressive decrease in the number of animals showing successful anamnestic responses and in the magnitude of those responses. It is concluded that S III depletes the antigen sensitive cell population in the secondary response largely because of its limited ability to stimulate sustained proliferation by such cells.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D002850:Chromatography, Gel; D005260:Female; D007106:Immune Sera / Q000302:isolation & purification / Q000494:pharmacology; D007114:Immunization; D007122:Immunoelectrophoresis; D007456:Iodine Isotopes; D011135:Polysaccharides, Bacterial; D011817:Rabbits; D013296:Streptococcus pneumoniae / Q000276:immunology*; D013997:Time Factors
D000906:Antibodies; D007106:Immune Sera; D007456:Iodine Isotopes; D011135:Polysaccharides, Bacterial