The stereochemistry of hydrogen transfer from reduced nicotinamide-adenine dinucleotide phosphate in the reduction of ethylenic linkages during cholesterol biosynthesis.
It is shown that during the saturation of steroid carbon-carbon double bonds at Delta(24,25) and Delta(14,15) the ;hydride ion' originates from the 4B side of the NADPH.
D000475:Alkenes / Q000378:metabolism*; D000818:Animals; D002784:Cholesterol / Q000096:biosynthesis*; D006859:Hydrogen / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D009249:NADP / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D014316:Tritium
D000475:Alkenes; D014316:Tritium; D009249:NADP; D006859:Hydrogen; D002784:Cholesterol