Isoprenoid biosynthesis in a cell-free system from pea shoots.
A cell-free system consisting of a soluble fraction and plastid fragments from pea shoots incorporates 2-(14)C-mevalonate very actively into farnesol, squalene, geranylgeraniol, and other isoprenoids of different carbon-chain lengths. The products have different cofactor requirements, which makes it possible to channel the pathway into different products by varying the incubation mixture.
D002247:Carbon Isotopes; D002855:Chromatography, Thin Layer; D005204:Farnesol / Q000096:biosynthesis; D066298:In Vitro Techniques; D008055:Lipids / Q000032:analysis; D008798:Mevalonic Acid / Q000378:metabolism*; D009243:NAD; D009249:NADP; D010945:Plants, Edible / Q000378:metabolism*; D013185:Squalene / Q000096:biosynthesis*; D013729:Terpenes / Q000096:biosynthesis*
D002247:Carbon Isotopes; D008055:Lipids; D013729:Terpenes; D009243:NAD; D005204:Farnesol; D009249:NADP; D013185:Squalene; D008798:Mevalonic Acid