D-amino acid oxidase in leukocytes: a possible D-amino-acid-linked antimicrobial system.
D-Amino acid oxidase has been identified within the granule fraction of human neutrophilic leukocytes. Leukocyte homogenates and purified kidney D-amino acid oxidase can utilize either isolated D-amino acids or some species of bacteria as substrates for the generation of hydrogen peroxide. When linked to leukocyte myeloperoxidase in vitro, purified D-amino acid oxidase constitutes a system lethal for certain bacteria. It is proposed that leukocyte D-amino acid oxidase and myeloperoxidase constitute a biochemically specific system for the recognition and killing of certain microorganisms.
D000818:Animals; D000900:Anti-Bacterial Agents; D003605:D-Amino-Acid Oxidase / Q000097:blood / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects; D006168:Guinea Pigs; D006801:Humans; D006861:Hydrogen Peroxide / Q000096:biosynthesis; D007962:Leukocytes / Q000201:enzymology*; D008722:Methods; D009504:Neutrophils / Q000201:enzymology; D010544:Peroxidases / Q000494:pharmacology; D011511:Proteus / Q000187:drug effects; D012706:Serratia marcescens / Q000187:drug effects; D013210:Staphylococcus / Q000187:drug effects
D000900:Anti-Bacterial Agents; D006861:Hydrogen Peroxide; D010544:Peroxidases; D003605:D-Amino-Acid Oxidase