Neuraminidase activity in Diplococcus pneumoniae.
Kelly, R. T. (Marquette University School of Medicine, Milwaukee, Wis.), D. Greiff, and S. Farmer. Neuraminidase activity in Diplococcus pneumoniae. J. Bacteriol. 91:601-603. 1966.-A method for the quantitation of neuraminidase in the presence of N-acetylneuraminic acid aldolase is described. The neuraminidase content of Diplococcus pneumoniae was found to be dependent on the media employed for growth; the highest enzyme activity per milligram of bacterial protein was obtained with Todd-Hewitt broth. Neuraminidase production was stimulated in D. pneumoniae by the addition of N-acetylneuraminlactose, N-acetylneuraminic acid, or N-acetylmannosamine to the growth medium. Three rough strains of D. pneumoniae, which were nonpathogenic for mice, lacked neuraminidase activity. Seven of 12 smooth strains contained neuraminidase; enzyme activity was not detected in the remaining 5 smooth strains. There was no correlation between the presence of neuraminidase activity and the capsular type or between neuraminidase production and animal virulence.
D000588:Amines / Q000494:pharmacology; D000818:Animals; D066298:In Vitro Techniques; D007785:Lactose / Q000494:pharmacology; D008358:Mannose / Q000494:pharmacology; D051379:Mice; D009438:Neuraminic Acids / Q000494:pharmacology; D009439:Neuraminidase / Q000378:metabolism*; D013296:Streptococcus pneumoniae / Q000201:enzymology*
D000588:Amines; D009438:Neuraminic Acids; D009439:Neuraminidase; D007785:Lactose; D008358:Mannose