A new nicotinamide-adenine dinucleotide-dependent hydroaromatic dehydrogenase of Lactobacillus plantarum and its role in formation of (minus)t-3,t-4-dihydroxycyclohexane-c-1-carboxylate.
1. A new induced NAD-dependent hydroaromatic dehydrogenase was isolated from a cell-free extract of Lactobacillus plantarum 13a and purified 175-fold. 2. The enzyme catalyses the oxidation of (-)-quinate, (-)-shikimate, (-)-dihydroshikimate and (-)t-3,t-4-dihydroxycyclohexane-c-1-carboxylate with NAD(+), and the reverse action with NADH. 3. The K(m) values at the optimal pH10.0 for these substrates are 0.85, 0.75, 0.52 and 0.74mm respectively, and the corresponding values for NAD(+) are 0.45, 0.26, 0.34 and 0.36mm respectively. 4. The stereochemical requirements of the enzyme and the role it may play in the reduction of (-)-quinate to (-)t-3,t-4-dihydroxycyclohexane-c-1-carboxylate are discussed and a pathway is suggested.
D002498:Centrifugation; D011232:Chemical Precipitation; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D003509:Cyclohexanecarboxylic Acids / Q000096:biosynthesis*; D006836:Hydro-Lyases; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007778:Lactobacillus / Q000201:enzymology*; D009243:NAD; D010088:Oxidoreductases; D011801:Quinic Acid / Q000378:metabolism; D012765:Shikimic Acid / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet
D003509:Cyclohexanecarboxylic Acids; D011801:Quinic Acid; D009243:NAD; D012765:Shikimic Acid; D010088:Oxidoreductases; D006836:Hydro-Lyases