Growth of Pseudomonas C on C1 compounds: continuoous culture.
Pseudomonas C was grown in continuous culture on methanol, formaldehyde, or formate as sole carbon source. On methanol mu(max) = 0.49/h and yield constant (Y) = 0.54; on formaldehyde and on unsupplemented media, mu(max) was about 0.2/h and Y was 0.15, whereas addition of p-aminobenzoic acid, folic acid, serine, or glycine to the medium raised Y to about 0.26 to 0.29, and addition of p-aminobenzoic acid, folic acid, serine, nicotinamide adenine dinucleotide, and Tween 80 raised the yield to 0.35. On formate and on unsupplemented media, mu(max) = 0.2/h and Y = 0.02, whereas addition of 0.1 mM p-aminobenzoic acid increased mu(max) to about 0.47 and Y to about 0.23. At low cell concentrations or growth rates a beneficial effect of CO(2) was observed. Formaldehyde or formate, when added together with methanol, were utilized simultaneously with the methanol.
D062365:Aminobenzoates / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D002250:Carbon Radioisotopes; D005492:Folic Acid / Q000378:metabolism; D005557:Formaldehyde / Q000378:metabolism*; D005561:Formates / Q000378:metabolism*; D005998:Glycine / Q000378:metabolism; D000432:Methanol / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D011136:Polysorbates / Q000378:metabolism; D011549:Pseudomonas / Q000254:growth & development* / Q000378:metabolism; D012694:Serine / Q000378:metabolism
D062365:Aminobenzoates; D002250:Carbon Radioisotopes; D005561:Formates; D011136:Polysorbates; D009243:NAD; D002245:Carbon Dioxide; D005557:Formaldehyde; D012694:Serine; D005492:Folic Acid; D005998:Glycine; D000432:Methanol