Nicotinamide-adenine dinucleotides in acute liver injury induced by ethionine, and a comparison with the effects of salicylate.
1. The effects of single doses of ethionine or sodium salicylate on the nicotinamide-adenine dinucleotide content of rat liver have been studied. 2. There was no significant change in the sum of NAD+NADH(2) during the early period (0-2hr.) of the liver injury induced by ethionine but there was a decrease in this value of approx. 30% by 4hr. after administration. 3. Ethionine had no significant effect on the NADP+NADPH(2) during the first 2hr. period after administration. The sum then decreased to a value approx. 70% of the control by 3hr. after dosing but showed a partial recovery at the 4hr. period before decreasing again in later stages of the poisoning. 4. Salicylate produced a very rapid decrease in the NADP+NADPH(2) in the liver after intraperitoneal injection. After 1hr. the decrease was approx. 30% of the initial value; the sum slowly returned towards the normal range during the following 4hr. 5. A high parenteral dose of salicylate was found to cause only a small depression in the concentration of ATP in rat liver in contrast with the rapid depletion produced by ethionine. 6. These results are discussed in terms of the liver disturbances produced by ethionine and salicylate.
D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D056486:Chemical and Drug Induced Liver Injury; D005001:Ethionine / Q000633:toxicity*; D005260:Female; D008099:Liver / Q000032:analysis / Q000187:drug effects*; D009243:NAD / Q000032:analysis*; D009249:NADP / Q000032:analysis*; D051381:Rats; D012980:Sodium Salicylate / Q000633:toxicity*
D009243:NAD; D009249:NADP; D000255:Adenosine Triphosphate; D012980:Sodium Salicylate; D005001:Ethionine