Enzyme-coenzyme complexes of pyridine nucleotide-linked dehydrogenases.
Enzyme-reduced coenzyme binary complexes produce previously unreported shifts in the spectrum of the free coenzyme. These shifts give rise to difference spectra which resemble a general environmental change for reduced diphosphopyridine nucleotide (DPNH) in the glutamic dehydrogenase-DPNH complex, and indicate a more specific enzyme-coenzyme interaction for yeast alcohol dehydrogenase-DPNH, isocitrate dehydrogenase-TPNH, and lactic dehydrogenase-DPNH complexes.
D000429:Alcohol Oxidoreductases; D005969:Glutamate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D009243:NAD; D009249:NADP; D013053:Spectrophotometry
D009243:NAD; D009249:NADP; D000429:Alcohol Oxidoreductases; D007521:Isocitrate Dehydrogenase; D005969:Glutamate Dehydrogenase