Bovine gingival collagenase: demonstration and initial characterization.
A collagenase active against native collagen was found in culture fluids of bovine gingiva. The enzyme first appeared in the culture fluid after 1-2 days and could be harvested thereafter for at least 30 days. The collagenase attacked collagen fibrils and cleaved collagen in solution, resulting in reaction products 1/4 and 3/4 of the length of the original molecule. The enzyme was inhibited by serum, by EDTA and by cysteine. The molecular weight was estimated by gel filtration to be 63,000 daltons.
D000818:Animals; D002250:Carbon Radioisotopes; D002417:Cattle; D002850:Chromatography, Gel; D003094:Collagen / Q000378:metabolism; D046508:Culture Techniques; D003545:Cysteine / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005881:Gingiva / Q000201:enzymology* / Q000378:metabolism / Q000648:ultrastructure; D005883:Gingival Crevicular Fluid / Q000201:enzymology; D003012:Microbial Collagenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008854:Microscopy, Electron; D008970:Molecular Weight; D051381:Rats; D013997:Time Factors
D002250:Carbon Radioisotopes; D003094:Collagen; D004492:Edetic Acid; D003012:Microbial Collagenase; D003545:Cysteine