Multiple forms of bacterial NADP-specific isocitrate dehydrogenase.
Electrophoretically distinct forms of nicotinamide adenine dinucleotide phosphate-specific isocritrate dehydrogenase have been observed in extracts of Escherichia coli grown under different culture conditions. In glucose-grown cells, two distinct bands of isocitrate dehydrogenase activity were observed on polyacrylamide gels and have been completely resolved by employing ion-exchange chromatography. These multiple forms of the enzyme have been studied and their possible metabolic role is discussed.
D002852:Chromatography, Ion Exchange; D004589:Electrophoresis, Disc; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007521:Isocitrate Dehydrogenase / Q000032:analysis*; D007527:Isoenzymes / Q000032:analysis*; D009249:NADP / Q000378:metabolism*
D007527:Isoenzymes; D009249:NADP; D007521:Isocitrate Dehydrogenase; D005947:Glucose