[Oncogen infectious nucleoprotein and herpes virus hominis type 2 in stages of cervical cancer. Investigatious by indirect immunofluorescens].
Using HVH 2 antisera of rabbit (titre 1:256, dilution 1:10), antologous serum of cervix carcinoma patients (dilution 1:1 to 1:5) and allogenic serum of cervix carcinoma patients without HVH 2 antibodies (dilution 1:1 to 1:5), tumour material obtained from various cancerization stages of cervix carcinoma in 50 cases was incubated following trypsination to single cells with fluoresceine isothiocyanate labelled anti-human globulin and anti-rabbit globulin. Apart from HVH antigens significantly differing in quantity from one stage to the other as detected by immune fluorescence methods, a perinuclear distinct antigen, unrelated to HVH antigens, has also been demonstrated. The last mentioned one might be a specific tumour antigen or an embryonic antigen. The occurrence of an indirectly or directly HVH 2 inducing antigen is considered less probable.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera; D007668:Kidney; D009698:Nucleoproteins / Q000302:isolation & purification*; D011817:Rabbits / Q000276:immunology; D018139:Simplexvirus / Q000302:isolation & purification*; D002583:Uterine Cervical Neoplasms / Q000032:analysis* / Q000276:immunology* / Q000382:microbiology
D000951:Antigens, Neoplasm; D007106:Immune Sera; D009698:Nucleoproteins
Onkogen-infektiöses Nukleoproteid und Herpesvirus hominis 2 bei Stufen des Carcinoma cervicis uteri im Aspekt indirekter Immunfluoreszenz