title: Nicotinamide adenine dinucleotide and nicotinamide adenine dinucleotide phosphate-linked succinic semialdehyde dehydrogenases in a Pseudonomas species.
abstract: A Pseudomonas species grown on polyamines was found to have the following enzymes that can dehydrogenate succinic semialdehyde: a constitutive nicotinamide adenine dinucleotide phosphate (NADP)-linked dehydrogenase, an inducible NAD-linked dehydrogenase specific for succinic semialdehyde (EC 1.2.lb), and more than one inducible NAD-linked aminoaldehyde dehydrogenase which can act on succinic semialdehyde, 3-aminopropanal, and 4-aminobutanal. These enzymes have been separated from each other by ammonium sulfate precipitation, column chromatography on diethylaminoethyl-Sephadex, and electrophoresis on polyacrylamide gel. The level of NAD-linked succinic semialdehyde dehydrogenase in cells grown on various C and N sources has been determined and found to be as expected of an inducible enzyme with, however, two slight variations: the basal level of the enzyme in cells grown on Casamino Acids is relatively high and readily detectable, and the level of this enzyme is the same when the cells are grown on gamma-aminobutyrate with or without glucose and is, therefore, not subject to the classical glucose effect.
mesh_terms: D000447:Aldehydes / Q000378:metabolism; D011232:Chemical Precipitation; D002845:Chromatography; D003470:Culture Media; D004586:Electrophoresis; D009243:NAD / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology* / Q000378:metabolism; D013386:Succinates / Q000378:metabolism*
chemical_list: D000447:Aldehydes; D003470:Culture Media; D013386:Succinates; D009243:NAD; D009249:NADP; D010088:Oxidoreductases