Competent Diplococcus pneumoniae accept both single- and double-stranded deoxyribonucleic acid.
The transforming activity of fractionated complementary strands of Diplococcus pneumoniae deoxyribonucleic acid (DNA) bands at the position of fully denatured DNA in CsCl at pH 11.0, and is completely (> 99.8%) destroyed by digestion with exonuclease-I. These results prove that pure single strands transform the normally prepared competent cells of this species. Their efficiency is about 0.5% that of native DNA of comparable size.
D002586:Cesium; D002712:Chlorides; D004269:DNA, Bacterial / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*
D002712:Chlorides; D004269:DNA, Bacterial; D002586:Cesium; D003851:Deoxyribonucleases