The existence of an electrophilic component in the reaction catalysed by triose phosphate isomerase.
In the presence of triose phosphate isomerase, the substrate dihydroxyacetone phosphate is reduced stereoselectively by NaBH(4). The reduction of enzyme-bound substrate is almost completely or completely stereoselective and occurs about one order of magnitude faster than that in free solution. This acceleration implies a polarization of the carbonyl group when dihydroxyacetone phosphate is bound.
D000096:Acetone; D000818:Animals; D001894:Borohydrides; D002238:Carbohydrate Epimerases; D002645:Chickens; D005994:Glycerophosphates; D009243:NAD; D009943:Organophosphorus Compounds; D014306:Trioses; D014316:Tritium
D001894:Borohydrides; D005994:Glycerophosphates; D009943:Organophosphorus Compounds; D014306:Trioses; D009243:NAD; D014316:Tritium; D000096:Acetone; D002238:Carbohydrate Epimerases