Golgi beta-glucuronidase of androgen-stimulated mouse kidney.
Subcellular fractions were prepared from mouse kidney homogenates by differential and sucrose-gradient centrifugation. A fraction enriched in Golgi apparatus was obtained, which had considerably enriched galactosyltransferase and thiamin pyrophosphatase activities, and was morphologically typical of Golgi material. This preparation also had high beta-glucuronidase activity, which increased concomitantly with microsomal beta-glucuronidase activity during the specific stimulation of the enzyme in male mouse kidney after androgen administration. The degree of stimulation was much greater in the Golgi fraction. Gel-electrophoretic patterns of Golgi beta-glucuronidase resembled more closely those of the enzyme located within lysosomes, but contained minor bands similar to those described previously (Swank & Paigen, 1973) as characteristic of the microsomal enzyme. It was concluded that the Golgi complex is involved in the distribution of the enzyme after its synthesis to both lysosomal and microsomal fractions.
D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D005690:Galactose; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism*; D006056:Golgi Apparatus / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D006062:Gonadotropins / Q000494:pharmacology*; D006602:Hexosyltransferases / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology; D051379:Mice; D008854:Microscopy, Electron; D008861:Microsomes / Q000201:enzymology; D013834:Thiamine Pyrophosphatase / Q000378:metabolism; D013997:Time Factors
D000728:Androgens; D006062:Gonadotropins; D006602:Hexosyltransferases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D005952:Glucose-6-Phosphatase; D005966:Glucuronidase; D013834:Thiamine Pyrophosphatase; D005690:Galactose