Tyrosine transaminase induction by dexamethasone in a new rat liver cell line.
A cell line derived from normal, adult rat liver has been established; the cells are similar to hepatocytes, as shown by electron microscopy. The addition of dexamethasone to the culture medium induced a three- to sixfold increase in the specific activity of tyrosine alpha-ketoglutarate transaminase; this increase was inhibited by the simultaneous addition of cycloheximide or actinomycin D. The latter, when added to cells given prior treatment with dexamethasone, further enhanced the transaminase activity. Contact-inhibited cells showed a lower response to dexamethasone than exponentially growing cells.
D000818:Animals; D002460:Cell Line / Q000201:enzymology; D003260:Contact Inhibition; D046508:Culture Techniques; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects*; D008099:Liver / Q000201:enzymology*; D008854:Microscopy, Electron; D051381:Rats; D014444:Tyrosine Transaminase / Q000096:biosynthesis*
D003609:Dactinomycin; D003907:Dexamethasone; D003513:Cycloheximide; D014444:Tyrosine Transaminase