The reconstitution of functional respiratory chains in membranes from electron-transport-deficient mutants of Escherichia coli as demonstrated by quenching of atebrin fluorescence.
Measurements were made of electron-transport-dependent quenching of atebrin fluorescence in particles prepared from mutants of Escherichia coli unable to synthesize either ubiquinone or haem. Such quenching was either absent (in haem-deficient particles) or decreased (in ubiquinone-deficient particles), but was restored under conditions used to reconstitute oxidase activities. It is concluded that the reconstitution of oxidase activity, in both cases, is associated with the formation of a functional, proton-translocating, respiratory chain.
D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D003486:Cyanides / Q000494:pharmacology; D004579:Electron Transport; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D005456:Fluorescent Dyes; D009154:Mutation; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D011796:Quinacrine; D013050:Spectrometry, Fluorescence
D003486:Cyanides; D005456:Fluorescent Dyes; D009243:NAD; D011796:Quinacrine