The kinetics of coupled enzyme reactions. Applications to the assay of glucokinase, with glucose 6-phosphate dehydrogenase as coupling enzyme.
1. A theory is developed to account for the kinetics of coupled-enzyme reactions without assuming that the second reaction follows first-order kinetics. 2. A simple procedure is described for applying the theory to the practical design of enzyme assays. 3. The validity of the theory is confirmed for the assay of glucokinase with glucose 6-phosphate dehydrogenase as coupling enzyme. 4. The possibility of extending the theory to three or more coupled reactions is discussed.
D000818:Animals; D002846:Chromatography, Affinity; D004798:Enzymes; D005941:Glucokinase / Q000032:analysis*; D005954:Glucosephosphate Dehydrogenase; D007700:Kinetics; D008956:Models, Chemical; D009243:NAD; D051381:Rats; D013053:Spectrophotometry
D004798:Enzymes; D009243:NAD; D005954:Glucosephosphate Dehydrogenase; D005941:Glucokinase