title: Purification of tetrahydrofolate dehydrogenase by affinity chromatography.
mesh_terms: D000478:Alkylation; D000645:Ammonium Sulfate; D000818:Animals; D002021:Buffers; D002460:Cell Line; D011232:Chemical Precipitation; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D005492:Folic Acid; D005493:Folic Acid Antagonists; D006863:Hydrogen-Ion Concentration; D007939:Leukemia L1210 / Q000201:enzymology*; D008722:Methods; D008727:Methotrexate; D009249:NADP; D009994:Osmolar Concentration; D011134:Polysaccharides; D011485:Protein Binding; D013053:Spectrophotometry; D013762:Tetrahydrofolate Dehydrogenase / Q000302:isolation & purification*; D014466:Ultraviolet Rays
chemical_list: D002021:Buffers; D005493:Folic Acid Antagonists; D011134:Polysaccharides; D009249:NADP; D005492:Folic Acid; D013762:Tetrahydrofolate Dehydrogenase; D000645:Ammonium Sulfate; D008727:Methotrexate