title: Dihydrofolate reductase. Purification and characterization of the enzyme from an amethopterin-resistant mutant of Escherichia coli.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003488:Cyanogen Bromide; D003619:Dansyl Compounds; D004352:Drug Resistance, Microbial; D004586:Electrophoresis; D004589:Electrophoresis, Disc; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development; D005492:Folic Acid; D005493:Folic Acid Antagonists; D006863:Hydrogen-Ion Concentration; D008727:Methotrexate; D009243:NAD; D009249:NADP; D009994:Osmolar Concentration; D011189:Potassium Chloride; D012965:Sodium Chloride; D013762:Tetrahydrofolate Dehydrogenase / Q000032:analysis / Q000302:isolation & purification*; D014461:Ultracentrifugation
chemical_list: D000596:Amino Acids; D003619:Dansyl Compounds; D005493:Folic Acid Antagonists; D009243:NAD; D012965:Sodium Chloride; D009249:NADP; D011189:Potassium Chloride; D005492:Folic Acid; D013762:Tetrahydrofolate Dehydrogenase; D003488:Cyanogen Bromide; D008727:Methotrexate