Lysine biosynthesis in Saccharomyces. Conversion of  -aminoadipate into  -aminoadipic  -semialdehyde.
The reduction of alpha-aminoadipate to alpha-aminoadipic delta-semialdehyde by a cell-free extract of Saccharomyces is shown to be a three-step process. First the amino acid reacts with ATP to form an adenylyl derivative. Then the adenylyl derivative of alpha-aminoadipate is reduced in the presence of NADPH. In the third step the reduced adenylyl derivative of the amino acid is cleaved to form alpha-aminoadipic delta-semialdehyde. The presence of Mg(2+) is necessary for the first and second steps. The third step does not need any cofactors. The product of the first step was isolated by chromatography after incubating the cell-free extract of Saccharomyces with alpha-aminoadipate, ATP and Mg(2+). The isolated product was identified as an adenylyl derivative of alpha-aminoadipate and could be converted into alpha-aminoadipic delta-semialdehyde under the stated experimental conditions. The product of the second step was too unstable to be identified.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000272:Adipates / Q000378:metabolism*; D000447:Aldehydes / Q000096:biosynthesis; D000588:Amines / Q000378:metabolism; D002247:Carbon Isotopes; D002474:Cell-Free System; D002854:Chromatography, Paper; D008239:Lysine / Q000096:biosynthesis*; D008274:Magnesium / Q000494:pharmacology; D009249:NADP / Q000378:metabolism; D010759:Phosphorus Isotopes; D012440:Saccharomyces / Q000378:metabolism*; D013053:Spectrophotometry
D000272:Adipates; D000447:Aldehydes; D000588:Amines; D002247:Carbon Isotopes; D010759:Phosphorus Isotopes; D009249:NADP; D000255:Adenosine Triphosphate; D008274:Magnesium; D008239:Lysine