Enzymes of fructose metabolism in human liver.
The enzyme activities involved in fructose metabolism were measured in samples of human liver. On the basis of U/g of wet-weight the following results were found: ketohexokinase, 1.23; aldolase (substrate, fructose-1-phosphate), 2.08; aldolase (substrate, fructose-1,6-diphosphate), 3.46; triokinase, 2.07; aldehyde dehydrogenase (substrate, D-glyceraldehyde), 1.04; D-glycerate kinase, 0.13; alcohol dehydrogenase (nicotinamide adenine dinucleotide [NAD]) substrate, D-glyceraldehyde), 3.1; alcohol dehydrogenase (nicotinamide adenine dinucleotide phosphate [NADP]) (substrate, D-glyceraldehyde), 3.6; and glycerol kinase, 0.62. Sorbitol dehydrogenases (25.0 U/g), hexosediphosphatase (4.06 U/g), hexokinase (0.23 U/g), and glucokinase (0.08 U/g) were also measured. Comparing these results with those of the rat liver it becomes clear that the activities of alcohol dehydrogenases (NAD and NADP) in rat liver are higher than those in human liver, and that the values of ketohexokinase, sorbitol dehydrogenases, and hexosediphosphatase in human liver are lower than those values found in rat liver. Human liver contains only traces of glycerate kinase. The rate of fructose uptake from the blood, as described by other investigators, can be based on the activity of ketohexokinase reported in the present paper. In human liver, ketohexokinase is present in a four-fold activity of glucokinase and hexokinase. This result may explain the well-known fact that fructose is metabolized faster than glucose.
D000429:Alcohol Oxidoreductases / Q000032:analysis; D000818:Animals; D005260:Female; D005632:Fructose / Q000378:metabolism*; D006597:Fructose-Bisphosphatase / Q000032:analysis; D005941:Glucokinase / Q000032:analysis; D006593:Hexokinase / Q000032:analysis; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008297:Male; D009243:NAD / Q000032:analysis; D009249:NADP / Q000032:analysis; D010770:Phosphotransferases / Q000032:analysis; D051381:Rats
D009243:NAD; D005632:Fructose; D009249:NADP; D000429:Alcohol Oxidoreductases; D010770:Phosphotransferases; D006593:Hexokinase; D005941:Glucokinase; D006597:Fructose-Bisphosphatase