A novel olefinic rearrangement. The enzymic conversion of cholesta-7,9-dien-3 -ol into cholesta-8,14-dien-3 -ol.
1. [3alpha-(3)H]Cholesta-7,9-dien-3beta-ol is converted in high yield into cholesterol by a 10000g(av.) supernatant fraction of rat liver homogenate. 2. Incubation of cholesta-7,9-dien-3beta-ol with [4-(3)H]NADPH and rat liver microsomal fractions under anaerobic conditions resulted in (3)H being incorporated into the 14alpha-position of cholest-7-en-3beta-ol. 3. Under anaerobic conditions in the absence of NADPH cholesta-7,9-dien-3beta-ol was isomerized into cholesta-8,14-dien-3beta-ol by rat liver microsomal fractions.
D000693:Anaerobiosis; D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D003897:Desmosterol / Q000378:metabolism; D007535:Isomerases; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D051381:Rats; D013261:Sterols / Q000378:metabolism*; D014316:Tritium
D013261:Sterols; D014316:Tritium; D003897:Desmosterol; D009249:NADP; D002784:Cholesterol; D007535:Isomerases