title: Altered fatty acid distribution in mutants of Neurospora crassa.
abstract: Morphological mutants of Neurospora with decreased levels of reduced nicotinamide adenine dinucleotide phosphate (NADPH) and reduced nicotinamide ad enine dinucleotide (NADH) contained only 20% as much of a polyunsaturated fatty acid (linolenic acid) as the wild type in both the phospholipid and neutral lipid fractions. There was an excellent correlation between linolenic acid levels and morphological appearance as a function of total NADPH content, but no correlation with NADH content. The linolenic acid deficiency was balanced by a relative increase in the amounts of the less unsaturated fatty acids (oleic and linoleic acids), but the level of three other fatty acids did not appear to be changed. This accumulation of these two precursors suggests that the NADPH deficiency preferentially affected the final desaturation step, i.e., the conversion of linoleic to linolenic acid. The NADPH needed for this reaction in vivo was probably generated by the pentose phosphate shunt, since mutations affecting the shunt lead to the decreased levels of linolenic acid. It is not clear whether the changes in fatty acid distribution affect the morphogenesis of Neurospora, or if these changes are just part of the NADPH-deficiency syndrome.
mesh_terms: D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis*; D005228:Fatty Acids, Essential / Q000032:analysis; D005612:Freeze Drying; D005827:Genetics, Microbial; D008041:Linoleic Acids / Q000032:analysis; D008055:Lipids / Q000032:analysis; D009154:Mutation; D009243:NAD / Q000032:analysis; D009249:NADP / Q000032:analysis; D009491:Neurospora / Q000032:analysis* / Q000254:growth & development; D009829:Oleic Acids / Q000032:analysis; D010169:Palmitic Acids / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D013229:Stearic Acids / Q000032:analysis
chemical_list: D005227:Fatty Acids; D005228:Fatty Acids, Essential; D008041:Linoleic Acids; D008055:Lipids; D009829:Oleic Acids; D010169:Palmitic Acids; D010743:Phospholipids; D013229:Stearic Acids; D009243:NAD; D009249:NADP