title: Competent Diplococcus pneumoniae accept both single- and double-stranded deoxyribonucleic acid.
abstract: The transforming activity of fractionated complementary strands of Diplococcus pneumoniae deoxyribonucleic acid (DNA) bands at the position of fully denatured DNA in CsCl at pH 11.0, and is completely (> 99.8%) destroyed by digestion with exonuclease-I. These results prove that pure single strands transform the normally prepared competent cells of this species. Their efficiency is about 0.5% that of native DNA of comparable size.
mesh_terms: D002586:Cesium; D002712:Chlorides; D004269:DNA, Bacterial / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*
chemical_list: D002712:Chlorides; D004269:DNA, Bacterial; D002586:Cesium; D003851:Deoxyribonucleases