5-Bromouridylyl-(3-5)-adenosine isolated from 5-bromouracil-induced filaments of Escherichia coli.
A dinucleoside monophosphate was isolated from 5-bromouracil-induced filaments of a thymine auxotroph of Escherichia coli K-12. The dinucleoside monophosphate was fractioned from a [(14)C]5-bromouracil-labeled perchloric acid extract using Dowex-1-formate ion-exchange chromatography. Sephadex chromatography revealed its molecular weight to be 710. Snake venom phosphodiesterase digest of the dinucleoside monophosphate yielded [(14)C]5-bromouridine and adenosine 5'-monophosphate. The presence of [(14)C]5-bromouracil in bacterial ribonucleic acid indicates that ribonucleic acid, which had incorporated 5-bromouracil, was the probable source of this dinucleoside monophosphate, 5-bromouridylyl-(3' --> 5')-adenosine.
D000241:Adenosine / Q000302:isolation & purification*; D000818:Animals; D001966:Bromine / Q000302:isolation & purification; D001976:Bromouracil / Q000494:pharmacology*; D002250:Carbon Radioisotopes; D002848:Chromatography, DEAE-Cellulose; D002854:Chromatography, Paper; D003470:Culture Media; D004926:Escherichia coli / Q000187:drug effects*; D006868:Hydrolysis; D008970:Molecular Weight; D009711:Nucleotides / Q000302:isolation & purification; D010727:Phosphoric Diester Hydrolases; D012329:RNA, Bacterial / Q000302:isolation & purification; D012260:Ribonucleases; D012911:Snakes; D014500:Uracil Nucleotides / Q000302:isolation & purification*; D014688:Venoms
D002250:Carbon Radioisotopes; D003470:Culture Media; D009711:Nucleotides; D012329:RNA, Bacterial; D014500:Uracil Nucleotides; D014688:Venoms; D001976:Bromouracil; D012260:Ribonucleases; D010727:Phosphoric Diester Hydrolases; D000241:Adenosine; D001966:Bromine