Appearance of T-cell markers in bone marrow rosette-forming cells after incubation with thymosin, a thymic hormone.
After incubation with thymosin, a thymic hormone, normal bone marrow rosette-forming cells acquire T-cell characteristics, including increased sensitivity to azathioprine, anti-lymphocyte serum, and anti-theta serum. This activity of thymosin provides a new sensitive and reproducible bioassay for thymosin, and is well correlated with an in vivo graft-versus-host assay. In addition, incubation of spleen cells from adult thymectomized mice with thymosin in vitro restores to normal their diminished sensitivity to azathioprine and anti-lymphocte serum.
D000818:Animals; D000921:Antibody-Producing Cells; D000961:Antilymphocyte Serum / Q000494:pharmacology; D001379:Azathioprine / Q000494:pharmacology; D001681:Biological Assay; D001853:Bone Marrow / Q000187:drug effects* / Q000276:immunology; D001854:Bone Marrow Cells; D001921:Brain / Q000276:immunology; D002417:Cattle; D004731:Endotoxins; D006087:Graft vs Host Reaction; D006728:Hormones / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D011817:Rabbits; D012475:Salmonella / Q000276:immunology; D012756:Sheep; D013154:Spleen / Q000276:immunology; D013552:Swine; D013934:Thymectomy; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism
D000961:Antilymphocyte Serum; D004731:Endotoxins; D006728:Hormones; D007106:Immune Sera; D001379:Azathioprine