Bone marrow-derived lymphoid cells (B cells): functional depletion with cobra factor and fresh serum.
Treatment of rat spleen cells with cobra factor and fresh rat serum provided a simple, rapid means of functionally eliminating complement receptor lymphocytes. Cells able to differentiate into plaque-forming cells in a syngeneic, irradiated host were diminished, but cells able to induce a graft-versus-host reaction were not diminished. There was no effect on plaque-forming cells from an immune spleen.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D000921:Antibody-Producing Cells / Q000187:drug effects; D000937:Antigen-Antibody Reactions / Q000187:drug effects; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D001665:Binding Sites; D001769:Blood; D003165:Complement System Proteins; D006087:Graft vs Host Reaction / Q000187:drug effects; D006462:Hemolytic Plaque Technique; D011828:Radiation Chimera; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000166:cytology; D014688:Venoms / Q000494:pharmacology*
D014688:Venoms; D003165:Complement System Proteins