Correlated changes of some enzyme activities and cofactor and substrate contents of pea cotyledon tissue during germination.
1. The activities of six enzymes (hexokinase, phosphoglucose isomerase, phosphofructokinase, aldolase, glucose 6-phosphate dehydrogenase and amylase) in extracts of pea cotyledons were determined. The activities during the first 10 days after germination showed individual and characteristic changes that indicate a specific control of both synthesis and destruction of enzymes. 2. Tissue contents of glucose, inorganic phosphate, glucose 6-phosphate, fructose 6-phosphate, ATP, ADP, AMP, NAD and NADP were also determined, and a correlation is reported between the substrate concentrations at day 1 and the subsequent enzymic activity. 3. The initial NAD(+)/NADH ratio value of 1 changed to about 3 by day 4; the NADP content was lower and changes in the oxidation state were less striking. The ratio of ATP to ADP and AMP remained virtually constant.
D000227:Adenine Nucleotides / Q000032:analysis; D000446:Aldehyde-Lyases; D000681:Amylases; D002245:Carbon Dioxide / Q000032:analysis; D002247:Carbon Isotopes; D003609:Dactinomycin / Q000494:pharmacology; D004798:Enzymes / Q000378:metabolism*; D005947:Glucose / Q000032:analysis; D005956:Glucose-6-Phosphate Isomerase; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase; D007700:Kinetics; D009243:NAD / Q000032:analysis; D009249:NADP / Q000032:analysis; D010710:Phosphates / Q000032:analysis; D010734:Phosphogluconate Dehydrogenase; D063245:Plant Development; D010936:Plant Extracts / Q000032:analysis; D010944:Plants / Q000201:enzymology*; D010945:Plants, Edible / Q000378:metabolism; D013997:Time Factors; D014166:Transferases
D000227:Adenine Nucleotides; D002247:Carbon Isotopes; D004798:Enzymes; D010710:Phosphates; D010936:Plant Extracts; D009243:NAD; D002245:Carbon Dioxide; D003609:Dactinomycin; D009249:NADP; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D014166:Transferases; D006593:Hexokinase; D000681:Amylases; D000446:Aldehyde-Lyases; D005956:Glucose-6-Phosphate Isomerase; D005947:Glucose