The effect of nickel on secretory systems. Studies on the release of amylase, insulin and growth hormone.
The effects of Ni(2+) on the release of amylase from rat parotids, insulin from mouse pancreatic islets and growth hormone from bovine pituitary slices were investigated. In all these secretory systems, Ni(2+) was shown to inhibit release evoked by a variety of stimuli both physiological and pharmacological. Measurements of rates of substrate oxidation and tissue concentrations of ATP and 3':5'-cyclic AMP suggest that this inhibitory action of Ni(2+) does not arise through an effect on energy metabolism or cyclic AMP metabolism. It is concluded that although some effects of Ni(2+) may involve antagonism between Ni(2+) and Ca(2+) in stimulus-secretion coupling, others appear to be independent of Ca(2+). It is suggested that Ni(2+) may block exocytosis by interfering with either secretory-granule migration or membrane fusion and microvillus formation. The possible mode of action of Ni(2+) and its potential use as a tool in the study of exocytosis are discussed.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000681:Amylases / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002250:Carbon Radioisotopes; D002417:Cattle; D000242:Cyclic AMP / Q000378:metabolism; D005089:Exocytosis; D005260:Female; D013006:Growth Hormone / Q000378:metabolism*; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism; D008297:Male; D051379:Mice; D009532:Nickel / Q000494:pharmacology*; D010306:Parotid Gland / Q000378:metabolism; D010902:Pituitary Gland / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D051381:Rats; D012636:Secretory Rate / Q000187:drug effects
D002250:Carbon Radioisotopes; D007328:Insulin; D009532:Nickel; D000255:Adenosine Triphosphate; D013006:Growth Hormone; D000242:Cyclic AMP; D000681:Amylases; D011188:Potassium; D002118:Calcium