Synthesis of inducible tyrosine aminotransferase in a cell-free extract from cultured hepatoma cells.
A cell-free extract from cultured rat hepatoma cells is described that incorporates amino acids into chains of tyrosine aminotransferase that are identical, by several criteria, with tyrosine aminotransferase from rat liver. Extracts from steroid-induced cells are 6-to 10-times more active for tyrosine aminotransferase synthesis than extracts from uninduced cells, although the total incorporation rates are identical.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000201:enzymology*; D002458:Cell Fractionation; D002460:Cell Line; D002474:Cell-Free System; D002848:Chromatography, DEAE-Cellulose; D003907:Dexamethasone / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction / Q000187:drug effects; D008113:Liver Neoplasms; D008970:Molecular Weight; D009374:Neoplasms, Experimental / Q000201:enzymology; D011132:Polyribosomes / Q000378:metabolism; D011233:Precipitin Tests; D011863:Radioimmunoassay; D051381:Rats; D014316:Tritium; D014444:Tyrosine Transaminase / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification
D000596:Amino Acids; D014316:Tritium; D003907:Dexamethasone; D014444:Tyrosine Transaminase