Isozyme patterns and sexual morphogenesis in Schizophyllum.
Isozymes of several different classes of enzymes in partially purified protein extracts of five strains of Schizophyllum commune, isogenic except for genes controlling sexual morphogenesis, were separated on polyacrylamide gel by disc electrophoresis. After staining, isozyme patterns were compared on the bases of the presence or absence, electrophoretic mobility (R(f) values), and relative activities of specific isozymes. Differences in isozyme patterns in 14 enzymes, i.e., NADH-dehydrogenase, NADPH-dehydrogenase, a number of NAD and NADP-dependent dehydrogenases, acid phosphatases, leucine aminopeptidase, and esterases, were correlated with the operation or inactivity of the A-and B-sequences of sexual morphogenesis. In only a single instance, i.e., phenolases, no marked differences could be correlated with sexual morphogenesis.
D000135:Acid Phosphatase / Q000032:analysis; D001487:Basidiomycota / Q000201:enzymology*; D002467:Cell Nucleus; D004589:Electrophoresis, Disc; D004950:Esterases / Q000032:analysis; D005827:Genetics, Microbial; D007527:Isoenzymes / Q000032:analysis*; D007931:Leucyl Aminopeptidase / Q000032:analysis; D009243:NAD; D009249:NADP; D010088:Oxidoreductases / Q000032:analysis*; D012737:Sex Factors
D007527:Isoenzymes; D009243:NAD; D009249:NADP; D010088:Oxidoreductases; D004950:Esterases; D000135:Acid Phosphatase; D007931:Leucyl Aminopeptidase