Deoxycytidylate deaminase evidence for a new enzyme in cells infected by the virus of herpes simplex.
The activities of dCMP deaminase and DNA polymerase I increased twofold and fivefold in BHK-21/C13 cells after infection by the virus of herpes simplex. The increases were greatly diminished, and under certain conditions prevented, by inclusion of actinomycin D or cycloheximide in the cell-virus system during the infective cycle. The dCMP deaminase purified from infected cells harvested 8h after infection differed from the deaminase purified from non-infected cells inasmuch as (a) it was more resistant to heating at 37 degrees C; (b) the substrate (dCMP) concentration at half-maximum velocity was lower; (c) maximum activation was achieved by a lower concentration of dCTP; (d) it was more resistant to inhibition by dTTP; and (e) it behaved differently when assayed in the presence of a herpes-virus-specific antiserum. The DNA polymerase activity in the infected cells was markedly decreased in the presence of the herpes-virus-specific antiserum.
D000619:Aminohydrolases / Q000302:isolation & purification*; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D006224:Cricetinae; D003513:Cycloheximide / Q000494:pharmacology; D003597:Cytosine Nucleotides; D004254:DNA Nucleotidyltransferases; D003609:Dactinomycin / Q000494:pharmacology; D003843:Deoxycytidine Monophosphate; D004790:Enzyme Induction; D006358:Hot Temperature; D007106:Immune Sera; D007668:Kidney; D007700:Kinetics; D018139:Simplexvirus / Q000201:enzymology* / Q000276:immunology
D003597:Cytosine Nucleotides; D007106:Immune Sera; D003843:Deoxycytidine Monophosphate; D003609:Dactinomycin; D003513:Cycloheximide; D004254:DNA Nucleotidyltransferases; D000619:Aminohydrolases