Mevalonate metabolism in supernatant fractions of lysates of Lactobacillus plantarum.
The supernatant fractions of lysates of Lactobacillus plantarum metabolize mevalonate into lipids. Adenosine triphosphate and uridine, as well as related compounds, and reduced nicotinamide adenine dinucleotide phosphate or reduced nicotinamide adenine dinucleotide stimulate this process. To obtain very active supernatant fractions, the method of lysis is modified to include polyamines during lysozyme treatment of cells and subsequent shocking with citrate buffer.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000588:Amines / Q000494:pharmacology; D002021:Buffers; D002247:Carbon Isotopes; D002474:Cell-Free System; D002855:Chromatography, Thin Layer; D007778:Lactobacillus / Q000378:metabolism*; D008798:Mevalonic Acid / Q000378:metabolism*; D009113:Muramidase / Q000378:metabolism; D009243:NAD / Q000494:pharmacology; D009249:NADP / Q000494:pharmacology; D013268:Stimulation, Chemical; D014529:Uridine / Q000494:pharmacology
D000588:Amines; D002021:Buffers; D002247:Carbon Isotopes; D009243:NAD; D009249:NADP; D000255:Adenosine Triphosphate; D009113:Muramidase; D008798:Mevalonic Acid; D014529:Uridine