An improved manual and semi-automatic assay for NADP-dependent isocitrate dehydrogenase activity, with a description of some kinetic properties of human liver and serum enzyme.
D002021:Buffers; D004789:Enzyme Activation; D006506:Hepatitis A / Q000201:enzymology; D006801:Humans; D007521:Isocitrate Dehydrogenase / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000097:blood; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008274:Magnesium; D008345:Manganese; D008722:Methods; D009249:NADP; D012965:Sodium Chloride; D013053:Spectrophotometry; D014466:Ultraviolet Rays
D002021:Buffers; D008345:Manganese; D012965:Sodium Chloride; D009249:NADP; D007521:Isocitrate Dehydrogenase; D008274:Magnesium