Fatty acid synthesis in intestinal mucosa of guinea pig.
1. Acetate-CoA ligase, acetyl-CoA-carbon dioxide ligase and fatty acid synthetase were shown to be present in particle-free fractions of guinea-pig intestinal mucosa. 2. Each of these enzymes was partially purified by ammonium sulphate precipitation from the particle-free supernatant. 3. The incorporation of acetate and citrate into fatty acid was measured. 4. Gas-liquid radiochromatography was used to investigate the pattern of fatty acids synthesized. 5. The rate-limiting step in fatty acid synthesis was shown to be acetyl-CoA-carbon dioxide ligase.
D000085:Acetates; D000818:Animals; D002247:Carbon Isotopes; D011232:Chemical Precipitation; D002849:Chromatography, Gas; D002951:Citrates; D004529:Egg White; D005227:Fatty Acids; D006168:Guinea Pigs; D007413:Intestinal Mucosa / Q000201:enzymology; D008025:Ligases; D009243:NAD; D009249:NADP; D011506:Proteins
D000085:Acetates; D002247:Carbon Isotopes; D002951:Citrates; D005227:Fatty Acids; D011506:Proteins; D009243:NAD; D009249:NADP; D008025:Ligases