title: The existence of an electrophilic component in the reaction catalysed by triose phosphate isomerase.
abstract: In the presence of triose phosphate isomerase, the substrate dihydroxyacetone phosphate is reduced stereoselectively by NaBH(4). The reduction of enzyme-bound substrate is almost completely or completely stereoselective and occurs about one order of magnitude faster than that in free solution. This acceleration implies a polarization of the carbonyl group when dihydroxyacetone phosphate is bound.
mesh_terms: D000096:Acetone; D000818:Animals; D001894:Borohydrides; D002238:Carbohydrate Epimerases; D002645:Chickens; D005994:Glycerophosphates; D009243:NAD; D009943:Organophosphorus Compounds; D014306:Trioses; D014316:Tritium
chemical_list: D001894:Borohydrides; D005994:Glycerophosphates; D009943:Organophosphorus Compounds; D014306:Trioses; D009243:NAD; D014316:Tritium; D000096:Acetone; D002238:Carbohydrate Epimerases