Structure-disrupting ions: detection of qualitative change in an enzyme.
Preparations of erythrocyte glucose-6-phosphate dehydrogenase from normal individuals show similar degrees of inhibition by neutral salts. Preparations from Caucasian subjects with different hereditary deficiency syndromes differ from each other and from normal subjects, indicating a qualitative difference in these specific "deficient" enzymes. Sensitivity to neutral salts may be a means of detecting an amino acid substitution.
D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002621:Chemistry; D003956:Dialysis; D004492:Edetic Acid; D005954:Glucosephosphate Dehydrogenase; D005955:Glucosephosphate Dehydrogenase Deficiency; D008623:Mercaptoethanol; D009249:NADP; D012492:Salts; D012964:Sodium; D012965:Sodium Chloride; D013053:Spectrophotometry
D012492:Salts; D012965:Sodium Chloride; D009249:NADP; D008623:Mercaptoethanol; D004492:Edetic Acid; D012964:Sodium; D005954:Glucosephosphate Dehydrogenase