Occurrence of 19S and 7S anti-IgGs during hyperimmunization of rabbits with streptococci.
All 110 rabbits immunized with Group A, A-variant, and C streptococcal vaccines produced 19S anti-IgG in addition to antibodies to the streptococcal carbohydrates. 19S anti-IgG was detected by hemagglutination of rabbit red blood cells coated with rabbit anti-blood group F antibody. Antisera of 88 of these animals were also tested for 7S anti-IgG with a coprecipitation assay. This assay is based on the coprecipitation of 7S anti-IgG with complexes of streptococcal carbohydrate and anti-carbohydrate antibody. 50 of the 88 anti-Group C streptococcal antisera contained 7S anti-IgGs. In eight antisera the concentration was greater than 5 mg/ml. The data suggest a genetic influence on the occurrence of 7S anti-IgG. The eight rabbits which produced more than 5 mg/ml of 7S anti-IgG belonged to three related families. Moreover, there were families in which almost every member produced 7S anti-IgG and other families in which only 30% of the members manufactured 7S anti-IgG. The streptococcal vaccine was an especially efficient stimulus for the production of 19S anti-IgG, whereas the pneumococcal vaccine was much less effective in this respect. Furthermore, 7S anti-IgGs were not detected in antipneumococcal antisera, although the concentration of anti-capsular antibodies was similar to that of anti-carbohydrate antibodies in antistreptococcal antisera.
D000818:Animals; D000917:Antibody Formation; D000936:Antigen-Antibody Complex / Q000032:analysis; D000941:Antigens; D001428:Bacterial Vaccines; D006041:Goats / Q000276:immunology; D006386:Hemagglutination Tests; D007106:Immune Sera; D007117:Immunization, Secondary; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007075:Immunoglobulin M / Q000096:biosynthesis*; D011135:Polysaccharides, Bacterial; D011234:Precipitins / Q000032:analysis; D011506:Proteins / Q000032:analysis; D011817:Rabbits; D013291:Streptococcus / Q000276:immunology*; D013296:Streptococcus pneumoniae / Q000276:immunology; D014461:Ultracentrifugation
D000936:Antigen-Antibody Complex; D000941:Antigens; D001428:Bacterial Vaccines; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011135:Polysaccharides, Bacterial; D011234:Precipitins; D011506:Proteins