title: Microtubule-macrotubule transitions: intermediates after exposure to the mitotic inhibitor vinblastine.
abstract: Vinblastine treatment of microtubule protein or intact microtubules assembled in vitro produced bifilar rings and bifilar helices. Suspentsions of rings and helices were demonstrated to bind [(3)H]colchicine, a diagnostic property of microtubule protein. Macrotubules are suggested to consist of tightly coiled helices formed by longitudinal compacting of loosely coiled protofilament pair intermediates.
mesh_terms: D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000648:ultrastructure; D003078:Colchicine / Q000378:metabolism; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D008870:Microtubules / Q000187:drug effects* / Q000648:ultrastructure; D008961:Models, Structural; D009419:Nerve Tissue Proteins / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D013552:Swine; D014316:Tritium; D014747:Vinblastine / Q000494:pharmacology*
chemical_list: D046911:Macromolecular Substances; D009419:Nerve Tissue Proteins; D014316:Tritium; D014747:Vinblastine; D003078:Colchicine