Purification and characterization of thermostable 5,10-methylenetetrahydrofolate dehydrogenase from Clostridium thermoaceticum.
D000596:Amino Acids / Q000032:analysis; D000645:Ammonium Sulfate; D001667:Binding, Competitive; D002474:Cell-Free System; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003013:Clostridium / Q000201:enzymology*; D004355:Drug Stability; D004589:Electrophoresis, Disc; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D046911:Macromolecular Substances; D008722:Methods; D008970:Molecular Weight; D009243:NAD; D009249:NADP; D010088:Oxidoreductases / Q000032:analysis / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate; D013696:Temperature; D013763:Tetrahydrofolates; D014461:Ultracentrifugation
D000596:Amino Acids; D046911:Macromolecular Substances; D013763:Tetrahydrofolates; D009243:NAD; D012967:Sodium Dodecyl Sulfate; D009249:NADP; D010088:Oxidoreductases; D000645:Ammonium Sulfate