title: The cooperative binding of two calcium ions to the double site of apothermolysin.
mesh_terms: D001059:Apoproteins; D001665:Binding Sites; D002118:Calcium / Q000032:analysis; D002850:Chromatography, Gel; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008433:Mathematics; D009994:Osmolar Concentration; D011485:Protein Binding; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013820:Thermolysin; D015032:Zinc / Q000032:analysis
chemical_list: D001059:Apoproteins; D013820:Thermolysin; D015032:Zinc; D002118:Calcium