Deuterium micromapping of biological samples by using the D(T,n)4He reaction and plastic track detectors.
A technique has been developed to micromap deuterium by using the D(T,n)(4)He reaction and plastic track detectors. Labeling of cells with subpicogram quantities of deuterium was demonstrated. The technique was used to localize human lymphocytes transformed in vitro.
D000512:Alpha Particles; D002708:Chlorella / Q000528:radiation effects; D002855:Chromatography, Thin Layer; D003903:Deuterium; D004912:Erythrocytes / Q000528:radiation effects; D006371:Helium; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D037102:Lectins / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects / Q000528:radiation effects; D013058:Mass Spectrometry; D009686:Nuclear Physics; D010784:Photomicrography; D011830:Radiation Effects; D011874:Radiometry; D013056:Spectrophotometry, Ultraviolet; D013936:Thymidine; D014316:Tritium
D037102:Lectins; D014316:Tritium; D006371:Helium; D003903:Deuterium; D013936:Thymidine