Homologous and hybrid complexes of anthranilate synthase from Bacillus species.
The subunits of anthranilate synthase were separated and partially purified by Sephadex G-100 gel filtration from the following six species of Bacillus: Bacillus subtilis, Bacillus licheniformis, Bacillus alvei, Bacillus coagulans, Bacillus pumilus, and Bacillus mascerans. Our data suggest that the enzyme from B. alvei is unique among these species. First, the anthranilate synthase complexes are readily dissociated during gel filtration in the absence of glutamine into a large component (aminotransferase), subunit E, and a small component subunit X (glutamine-binding protein), whereas a higher salt concentration is required to dissociate the complex from B. alvei. Second, the aminotransferase activity from all six species is stimulated by glycerol and inhibited by tryptophan; however, only the large component from B. alvei is stimulated by 2-mercaptoethanol. Finally, the large component can be titrated with the small component to yield a complex which can utilize glutamine as a substrate (amidotransferase). The homologous complexes have an amidotransferase to aminotransferase ratio of 1.4 to 2.3, but the B. alvei complex has a ratio of 0.9. Except for complexes that involve the large component from B. alvei, hybrid complexes can be formed which have ratios as good as the homologous complexes. These data are consistent with the hypothesis that B. alvei is unique among the bacilli with respect to some enzymes in the aromatic amino acid biosynthetic pathway.
D000577:Amides; D000878:Anthranilate Synthase / Q000302:isolation & purification* / Q000378:metabolism; D001407:Bacillus / Q000201:enzymology*; D002474:Cell-Free System; D002850:Chromatography, Gel; D005816:Genetic Complementation Test; D005973:Glutamine / Q000378:metabolism; D005990:Glycerol / Q000494:pharmacology; D006824:Hybridization, Genetic; D008623:Mercaptoethanol / Q000494:pharmacology; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000302:isolation & purification / Q000378:metabolism; D009154:Mutation; D013045:Species Specificity; D000637:Transaminases / Q000302:isolation & purification / Q000378:metabolism; D014166:Transferases / Q000302:isolation & purification / Q000378:metabolism; D014364:Tryptophan / Q000494:pharmacology
D000577:Amides; D009097:Multienzyme Complexes; D005973:Glutamine; D008623:Mercaptoethanol; D014364:Tryptophan; D014166:Transferases; D000637:Transaminases; D000878:Anthranilate Synthase; D005990:Glycerol