Cerebral hydroxylases: stimulation by a new factor.
Cerebral tryptophan 5-hydroxylase and tyrosine hydroxylase are stimulated by a solution of lyophilized, protein-free 30,000g supernatant of brain homogenates (concentrate 1). This preparation can be further purified by passage through Sephadex G-75 and phosphocellulose columns to yield concentrate 2. Unlike concentrate 1, concentrate 2 is unstable after several days of storage even at -27 degrees C. Neither preparation is active without the presence of a reduced pteridine cofactor. The stimulating factor appears to be thermostable, alkali-labile, dialyzable, and light-sensitive.
D000468:Alkalies; D000818:Animals; D001894:Borohydrides / Q000494:pharmacology; D001921:Brain / Q000201:enzymology*; D001923:Brain Chemistry; D002247:Carbon Isotopes; D002850:Chromatography, Gel; D003956:Dialysis; D004298:Dopamine / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D006358:Hot Temperature; D008623:Mercaptoethanol; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D010782:Photolysis; D011621:Pteridines; D011622:Pterins / Q000494:pharmacology; D051381:Rats; D014020:Tissue Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D014363:Tryptamines / Q000378:metabolism; D014365:Tryptophan Hydroxylase / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
D000468:Alkalies; D001894:Borohydrides; D002247:Carbon Isotopes; D011621:Pteridines; D011622:Pterins; D014020:Tissue Extracts; D014363:Tryptamines; D008623:Mercaptoethanol; D006899:Mixed Function Oxygenases; D014446:Tyrosine 3-Monooxygenase; D014365:Tryptophan Hydroxylase; D004298:Dopamine