A pyrrolidonecarboxylate peptidase from the particulate fraction of Klebsiella cloacae. Purification of the stable enzyme and its use in releasing the NH2 terminus from pyrrolidonecarboxylyl peptides and proteins.
D000626:Aminopeptidases / Q000302:isolation & purification* / Q000378:metabolism; D002413:Cations, Divalent; D002729:Chloromercuribenzoates / Q000494:pharmacology; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003080:Cold Temperature; D003460:Crystallization; D004355:Drug Stability; D004589:Electrophoresis, Disc; D004590:Electrophoresis, Paper; D006863:Hydrogen-Ion Concentration; D007461:Iodoacetates / Q000494:pharmacology; D007525:Isoelectric Focusing; D007700:Kinetics; D007709:Klebsiella / Q000201:enzymology*; D008970:Molecular Weight; D009904:Optical Rotation; D010618:Phenanthrolines / Q000494:pharmacology; D011092:Polyethylene Glycols; D011392:Proline; D011761:Pyrrolidonecarboxylic Acid; D011804:Quinolines / Q000494:pharmacology; D013329:Structure-Activity Relationship
D002413:Cations, Divalent; D002729:Chloromercuribenzoates; D007461:Iodoacetates; D010618:Phenanthrolines; D011804:Quinolines; D011092:Polyethylene Glycols; D011392:Proline; D000626:Aminopeptidases; D011761:Pyrrolidonecarboxylic Acid