Pseudomonas putida mutants defective in the metabolism of the products of meta fission of catechol and its methyl analogues.
A selection procedure is described which was used to isolate mutants of Pseudomonas putida strain U in the following enzymes of the meta-fission pathway of phenol and cresols: hydrolase, tautomerase, and decarboxylase. Strains deficient in the hydrolase are unable to use either o- or m-cresol as a sole carbon source and were shown to accumulate 2-hydroxy-6-keto-2,4-heptadienoate when incubated in the presence of o- or m-cresol. When 2-hydroxymuconic semialdehyde (plus nicotinamide adenine dinucleotide, oxidized form) was metabolized by phenol-induced extracts of tautomerase-deficient strains, the enol tautomer of 4-oxalocrotonate accumulated and was then converted slowly to the keto tautomer by a nonenzymatic reaction. Phenol-induced extracts of decarboxylase-deficient strains accumulated the keto tautomer of 4-oxalocrotonate from 2-hydroxymuconic semialdehyde (plus nicotinamide adenine dinucleotide, oxidized form). Strains with an inactive decarboxylase are unable to completely metabolize either phenol or p-cresol. Tautomerase-defective strains are unable to grow with p-cresol as the sole carbon source and grow only very slowly on phenol.
D000447:Aldehydes / Q000096:biosynthesis; D002262:Carboxy-Lyases / Q000378:metabolism; D003408:Cresols / Q000378:metabolism*; D003437:Crotonates / Q000096:biosynthesis; D003470:Culture Media; D003523:Cycloserine; D005650:Fumarates / Q000378:metabolism; D006867:Hydrolases / Q000378:metabolism; D007535:Isomerases / Q000378:metabolism; D009154:Mutation; D010400:Penicillin G; D010636:Phenols / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D013237:Stereoisomerism
D000447:Aldehydes; D003408:Cresols; D003437:Crotonates; D003470:Culture Media; D005650:Fumarates; D010636:Phenols; D003523:Cycloserine; D006867:Hydrolases; D002262:Carboxy-Lyases; D007535:Isomerases; D010400:Penicillin G