title: The isolation of plant protoplasts.
mesh_terms: D002458:Cell Fractionation; D002478:Cells, Cultured; D002480:Cellulase; D002498:Centrifugation; D002523:Edible Grain / Q000166:cytology; D005069:Evaluation Studies as Topic; D001467:Hordeum / Q000166:cytology; D006863:Hydrogen-Ion Concentration; D008722:Methods; D008854:Microscopy, Electron; D059828:Plant Cells; D010947:Plants, Toxic; D011523:Protoplasts / Q000648:ultrastructure; D012434:Secale / Q000166:cytology; D013045:Species Specificity; D014026:Nicotiana / Q000166:cytology; D014908:Triticum / Q000166:cytology
chemical_list: D002480:Cellulase