A rapid and efficient new method of purification of glutamate dehydrogenase by affinity chromatography on GTP-sepharose.
D000818:Animals; D001665:Binding Sites; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D005969:Glutamate Dehydrogenase / Q000302:isolation & purification*; D006160:Guanosine Triphosphate; D006882:Hydroxyapatites; D008722:Methods; D008930:Mitochondria, Liver / Q000201:enzymology; D008931:Mitochondria, Muscle / Q000201:enzymology; D009206:Myocardium; D009928:Organ Specificity; D011485:Protein Binding; D051381:Rats; D012685:Sepharose; D012967:Sodium Dodecyl Sulfate; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet; D013552:Swine; D013997:Time Factors; D014465:Ultrasonics
D006882:Hydroxyapatites; D012967:Sodium Dodecyl Sulfate; D006160:Guanosine Triphosphate; D012685:Sepharose; D005969:Glutamate Dehydrogenase