title: Temperate bacteriophage infectious for asporogenic variants of Bacillus pumilus.
abstract: Bacillus pumilus strain NRRL B-3275 is lysogenic for an inducible, nondefective temperate bacteriophage phi75. phi75 infects and lysogenizes several asporogenic mutants of B. pumilus strain NRS 576 but does not productively infect the spore(+) parent. phi75 DNA is a linear duplex with a mol wt of about 29 x 10(6) and a buoyant density of 1.701 g/cm(3). The location of the phi75 prophage attachment site on the chromosome of both host strains is adjacent to a lysine marker. The apparent order is phi75 att lys trp.
mesh_terms: D001407:Bacillus / Q000254:growth & development; D001435:Bacteriophages / Q000032:analysis / Q000254:growth & development* / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient; D002874:Chromosome Mapping; D004267:DNA Viruses; D004279:DNA, Viral / Q000032:analysis; D008242:Lysogeny; D008854:Microscopy, Electron; D008970:Molecular Weight; D009154:Mutation; D013171:Spores, Bacterial / Q000254:growth & development; D014161:Transduction, Genetic; D010948:Viral Plaque Assay; D014779:Virus Replication
chemical_list: D004279:DNA, Viral