Triton X-100-4 M urea as an extraction medium for membrane proteins. I. Purification of chloroplast cytochrome b559.
D001205:Ascorbic Acid; D001665:Binding Sites; D002462:Cell Membrane / Q000032:analysis*; D002736:Chloroplasts / Q000032:analysis* / Q000166:cytology; D002848:Chromatography, DEAE-Cellulose; D003580:Cytochromes / Q000302:isolation & purification*; D004229:Dithiothreitol; D004355:Drug Stability; D004589:Electrophoresis, Disc; D000431:Ethanol; D005990:Glycerol; D006863:Hydrogen-Ion Concentration; D046911:Macromolecular Substances; D009584:Nitrogen; D010084:Oxidation-Reduction; D010944:Plants; D011092:Polyethylene Glycols; D011485:Protein Binding; D011489:Protein Denaturation; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet; D013501:Surface-Active Agents; D013997:Time Factors; D014465:Ultrasonics; D014508:Urea
D003580:Cytochromes; D046911:Macromolecular Substances; D013501:Surface-Active Agents; D000431:Ethanol; D011092:Polyethylene Glycols; D014508:Urea; D009584:Nitrogen; D005990:Glycerol; D001205:Ascorbic Acid; D004229:Dithiothreitol