Partial purification of a membrane-associated deoxyribonucleic acid complex from Mycoplasma gallisepticum.
A Mycoplasma gallisepticum subcellular fraction (P2), which contains the deoxyribonucleic acid replication complex, can be isolated by differential centrifugation of freeze-thaw-lysed cells. The nascent deoxyribonucleic acid is released from P2 by Lubrol-WX, sodium dodecyl sulfate, Pronase, and deoxyribonuclease, but not by saponin, ribonuclease, phospholipase C, or high-frequency sonic treatment. Sonic treatment further fractionates the cell ghost and allows partial purification, on sucrose density gradients, of a deoxyribonucleic acid replication complex attached to the cells' polar membrane-bleb-infrableb structures.
D002250:Carbon Radioisotopes; D002462:Cell Membrane / Q000032:analysis; D002499:Centrifugation, Density Gradient; D004261:DNA Replication; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D003851:Deoxyribonucleases; D008854:Microscopy, Electron; D009174:Mycoplasma / Q000032:analysis*; D009829:Oleic Acids; D011402:Pronase; D012260:Ribonucleases; D013010:Sonication; D013347:Subcellular Fractions / Q000032:analysis; D013501:Surface-Active Agents; D013936:Thymidine; D014316:Tritium; D014529:Uridine
D002250:Carbon Radioisotopes; D004269:DNA, Bacterial; D009829:Oleic Acids; D013501:Surface-Active Agents; D014316:Tritium; D003851:Deoxyribonucleases; D012260:Ribonucleases; D011402:Pronase; D013936:Thymidine; D014529:Uridine