title: Preparation of immunologlobulin light-chain mRNA from microsomes without the use of detergent.
mesh_terms: D000818:Animals; D001345:Autoradiography; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003593:Cytoplasm / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007074:Immunoglobulin G / Q000096:biosynthesis; D066298:In Vitro Techniques; D008566:Membranes / Q000378:metabolism; D008722:Methods; D051379:Mice; D008861:Microsomes / Q000378:metabolism*; D008928:Mitochondria / Q000032:analysis; D009698:Nucleoproteins / Q000032:analysis; D010761:Phosphorus Radioisotopes; D010954:Plasmacytoma / Q000378:metabolism; D011691:Puromycin / Q000494:pharmacology; D012313:RNA / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D012260:Ribonucleases; D012270:Ribosomes / Q000378:metabolism; D014461:Ultracentrifugation
chemical_list: D007074:Immunoglobulin G; D009698:Nucleoproteins; D010761:Phosphorus Radioisotopes; D012333:RNA, Messenger; D011691:Puromycin; D012313:RNA; D012260:Ribonucleases