Liquid-gel partition chromatography of vitamin A compounds; formation of retinoic acid from retinyl acetate in vivo.
A clear separation of retinol, retinal, and retinoic acid has been achieved by liquid-gel partition chromatography on Sephadex LH-20 with solvent mixtures of chloroform, Skellysolve B, and methanol. A mixture of retinyl esters, retinol, retinal, and retinoic acid has been resolved on hydroxyalkoxypropyl Sephadex using Skellysolve B and acetone. There is no decomposition of any of the vitamin A compounds during chromatography, and recovery is complete. The combination of mildness and potential for resolution makes liquid-gel partition chromatography a superior tool for the separation of vitamin A compounds. This method has been applied to the study of vitamin A metabolism at physiological levels in the vitamin A-deficient rat. Retinyl palmitate, an ester of retinoic acid, retinal, retinol, retinoic acid, and a polar metabolite have been demonstrated in various tissues of the rat 12 hr after a dose of 2 micro g of [11-(14)C]retinyl acetate.
D000085:Acetates / Q000378:metabolism; D000818:Animals; D002250:Carbon Radioisotopes; D002338:Carotenoids / Q000096:biosynthesis*; D002850:Chromatography, Gel; D004987:Ethers; D005069:Evaluation Studies as Topic; D007421:Intestine, Small / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D008722:Methods; D010169:Palmitic Acids / Q000096:biosynthesis; D011134:Polysaccharides; D051381:Rats; D012867:Skin / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D014801:Vitamin A / Q000032:analysis* / Q000378:metabolism; D014802:Vitamin A Deficiency / Q000378:metabolism
D000085:Acetates; D002250:Carbon Radioisotopes; D004987:Ethers; D010169:Palmitic Acids; D011134:Polysaccharides; D014801:Vitamin A; D002338:Carotenoids