Purification and biophysical properties of acute haemorrhagic conjunctivitis virus.
The buoyant density of acute haemorrhagic conjunctivitis virions labeled with either [(3)H]uridine or [(3)H]leucine was 1.34 g/ml in CsCl and 1.25 g/ml in sucrose. RNA extracted from the virions gave a sedimentation coefficient of approximately 34S in sucrose, and was found to be sensitive to RNase. Molecular weight of RNA was calculated to be 2.5 x 10(6) using poliovirus RNA for reference.
D002499:Centrifugation, Density Gradient; D002501:Centrifugation, Zonal; D003231:Conjunctivitis / Q000382:microbiology*; D007930:Leucine; D008970:Molecular Weight; D012328:RNA Viruses / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*; D012367:RNA, Viral / Q000302:isolation & purification; D014316:Tritium; D014529:Uridine; D014779:Virus Replication
D012367:RNA, Viral; D014316:Tritium; D007930:Leucine; D014529:Uridine