Studies on the form and synthesis of messenger ribonucleic acid in the rat ventral prostate gland, including its tissue-specific stimulation by androgens.
1. When prostate polyribosomes are labelled with radioactive precursors in vivo and subsequently dissociated with sodium dodecyl sulphate, a heterogeneous 6-15S RNA species may be identified that possesses all of the distinctive properties of mRNA. 2. Apart from the selective incorporation of 5'-fluoro-orotic acid into this 6-15S RNA component, it is bound by nitrocellulose filters under experimental conditions where only poly(A)-rich species of RNA are specifically retained. Most importantly, however, only the 6-15S RNA fraction is capable of promoting the incorporation of amino acids into peptide linkage in an mRNA-depleted cell-free system derived from ascites-tumour cells. 3. With the development of a simpler method for labelling the total RNA fraction of the prostate gland in vitro, the poly(A)-enriched RNA fraction may be readily isolated by adsorption and elution from oligo(dT)-cellulose. The synthesis of the poly(A)-enriched 6-15S RNA fraction is stringently controlled by androgens in a highly tissue- and steroid-specific manner. 4. From an analysis of the proteins synthesized in the ascites cell-free system in the presence of the poly(A)-rich RNA fraction, it appears that protein synthesis in the prostate gland is stimulated in a rather general way, even during the earliest phases of the androgenic response. This conclusion may require modification when more specific means of analysis are available than those used in the present investigation. 5. The implications of these findings to the mechanism of action of androgens are discussed.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002250:Carbon Radioisotopes; D002287:Carcinoma, Krebs 2; D002369:Castration; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D007274:Injections, Intraperitoneal; D008297:Male; D009928:Organ Specificity; D009963:Orotic Acid / Q000378:metabolism; D011132:Polyribosomes / Q000032:analysis; D011467:Prostate / Q000187:drug effects / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000302:isolation & purification; D012335:RNA, Ribosomal; D012343:RNA, Transfer / Q000302:isolation & purification; D051381:Rats; D012967:Sodium Dodecyl Sulfate; D013462:Sulfur Radioisotopes; D013739:Testosterone / Q000494:pharmacology*; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D000596:Amino Acids; D002250:Carbon Radioisotopes; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D013462:Sulfur Radioisotopes; D014316:Tritium; D012967:Sodium Dodecyl Sulfate; D013739:Testosterone; D009963:Orotic Acid; D012343:RNA, Transfer; D014529:Uridine