Changes in Microsporum gypseum mycelial wall and spore coat glycoproteins during sporulation and spore germination.
Ethylenediamine-soluble glycoproteins were extracted from isolated Microsporum gypseum hyphal walls during sporulation and from spore coats before and after germination. This study was carried out to identify a sporulation-specific cell wall protein that possibly served as a substrate for the alkaline protease which initiated the macroconidial germination of this fungus. Analyses revealed that water-insoluble glycoprotein accounted for 10% of the ungerminated spore coat but only for 4 to 5% of the mycelial wall dry weight. This fraction was modified in its amino acid composition during sporulation, and it decreased in protein content during spore germination. Water-soluble glycoprotein, which accounted for approximately 3 to 3.5% of either the spore coat or mycelial wall dry weight, was of similar amino acid composition from both sources and did not decrease in protein content upon spore germination. The water-insoluble glycoprotein was found to be rich in leucine, aspartic acid, glycine, glutamic acid, and phenylalanine residues. The water-soluble glycoprotein was rich in proline, threonine, glycine, serine, glutamic acid, and alanine.
D000596:Amino Acids / Q000378:metabolism; D000606:Amino Sugars / Q000378:metabolism; D002458:Cell Fractionation; D002473:Cell Wall / Q000201:enzymology / Q000378:metabolism*; D002474:Cell-Free System; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism; D005029:Ethylenediamines; D005656:Fungal Proteins / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D006601:Hexoses / Q000378:metabolism; D008864:Microsporum / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D009154:Mutation; D012997:Solvents; D013172:Spores, Fungal / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D013997:Time Factors; D014867:Water
D000596:Amino Acids; D000606:Amino Sugars; D005029:Ethylenediamines; D005656:Fungal Proteins; D006023:Glycoproteins; D006601:Hexoses; D012997:Solvents; D014867:Water; D010450:Endopeptidases