Alkaline phosphatase from pig kidney. Microheterogeneity and the role of neuraminic acid.
Several alkaline phosphatases (EC 3.1.3.1) could be obtained from pig kidney brush-border membrane on extraction with butan-1-ol. Three of the multiple forms were separated by DEAE-cellulose chromatography and further purified. They form a regular series with different degrees of glycosylation (mainly owing to N-acetylneuraminic acid), of charge, of molecular weight, of stability to temperature, to pH and to urea, of minimal requirement for Mg(2+) and of extractability by butan-1-ol. In contrast, the detectable antigenic sites, the inhibition by amino acids and the pH-dependency of K(m) and V(max.) were identical for these multiple forms. On treatment with neuraminidase, the multiple forms became identical in all their properties. It was therefore concluded that the microheterogeneity of alkaline phosphatase is due to different degrees of glycosylation at polypeptide chains which appear to be otherwise identical.
D000469:Alkaline Phosphatase / Q000032:analysis*; D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004592:Electrophoresis, Starch Gel; D004789:Enzyme Activation; D006041:Goats / Q000276:immunology; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera; D007527:Isoenzymes / Q000032:analysis; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D008274:Magnesium; D008566:Membranes / Q000032:analysis; D008970:Molecular Weight; D009438:Neuraminic Acids / Q000032:analysis*; D009439:Neuraminidase; D013552:Swine; D013696:Temperature
D007106:Immune Sera; D007527:Isoenzymes; D009438:Neuraminic Acids; D000469:Alkaline Phosphatase; D009439:Neuraminidase; D008274:Magnesium