Quantitation of A2 hemoglobin by polyacrylamide gel disc electrophoresis: a method with individual specimen standardization.
A polyacrylamide gel electrophoresis technic for the determination of hemoglobin A2 is presented. Rapid separation is an advantage. The use of a diluted hemolysate as a 4 percent standard avoids overestimation of the A2 fraction by densitometry and also provides a reference for visual comparison. Normal range of A2 hemoglobin by this method is 1.12 to 4.13 per dkg. Coefficient of variation was 5.84 percent.
D004591:Electrophoresis, Polyacrylamide Gel; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D013789:Thalassemia / Q000097:blood* / Q000175:diagnosis
D006454:Hemoglobins