Leucyl-transfer ribonucleic acid synthetase in senescing tobacco leaves.
1. The activity of leucyl-tRNA synthetase obtained from tobacco leaves declined by 50% over a period of 4 days senescence induced by detachment. In addition tRNA(Leu) from senescing leaves was charged to a lesser extent than tRNA(Leu) extracted from mature leaves immediately after detachment. 2. tRNA(Leu) was charged with a synthetase preparation from either mature or senescent leaves and chromatographed on an RPC 3 column. The elution profile showed that the marked decline in specific activity of leucyl-tRNA synthetase in senescent leaves was not associated with a loss of acylation of any isoacceptor of tRNA(Leu).
D000375:Aging; D000604:Amino Acyl-tRNA Synthetases / Q000032:analysis*; D002498:Centrifugation; D002845:Chromatography; D002848:Chromatography, DEAE-Cellulose; D003956:Dialysis; D007930:Leucine; D010944:Plants / Q000201:enzymology*; D010947:Plants, Toxic; D014026:Nicotiana / Q000201:enzymology; D014316:Tritium
D014316:Tritium; D000604:Amino Acyl-tRNA Synthetases; D007930:Leucine