The effect of delta1-tetrahydrocannabinol on the uptake of [3H]-(minus)-noradrenaline by the isolated perfused heart of the rat.
Forty-eight isolated rat hearts were perfused with Krebs solution and with [(3)H]-(-)-noradrenaline ([(3)H]-NA) containing 4.5 nCi/ml in 50 ng/ml, as described by Iversen (1963). To groups of six, cocaine was added to [(3)H]-NA in concentrations of 400 nM, 2 and 10 muM. To the rest Tween 80, 25 mug/ml was added to [(3)H]-NA and in three groups Delta(1)-THC additionally in concentrations of 560 nM and 2.8 or 14 muM. Cocaine caused a linear concentration-related inhibition of uptake; Tween caused a 38.7% inhibition; THC caused additionally a linear concentration-related inhibition.
D000818:Animals; D002188:Cannabis / Q000494:pharmacology*; D003042:Cocaine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D013759:Dronabinol / Q000494:pharmacology*; D005260:Female; D006321:Heart; D066298:In Vitro Techniques; D009206:Myocardium / Q000378:metabolism*; D009638:Norepinephrine / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010477:Perfusion; D011136:Polysorbates / Q000494:pharmacology; D051381:Rats; D014316:Tritium
D011136:Polysorbates; D014316:Tritium; D013759:Dronabinol; D003042:Cocaine; D009638:Norepinephrine