title: Subcellular distribution and properties of kynurenine transaminase in rat liver.
abstract: Kynurenine transaminase activity in rat liver was found in both the mitochondrial and supernatant fractions. The mitochondrial and supernatant fractions contained (a) kynurenine-pyruvate transaminase, which showed a preference for pyruvate as amino acceptor and a pH optimum between 8.0 and 8.5, and (b) kynurenine-alpha-oxoglutarate transaminase, with a preference for alpha-oxoglutarate and a pH optimum between 6.0 and 6.5. Possible roles of these enzymes in tryptophan metabolism in the liver are discussed.
mesh_terms: D000429:Alcohol Oxidoreductases / Q000302:isolation & purification; D000818:Animals; D002845:Chromatography; D002848:Chromatography, DEAE-Cellulose; D006863:Hydrogen-Ion Concentration; D006882:Hydroxyapatites; D007656:Ketoglutaric Acids / Q000378:metabolism; D007737:Kynurenine; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D008930:Mitochondria, Liver / Q000201:enzymology; D008995:Monoamine Oxidase / Q000302:isolation & purification; D011773:Pyruvates / Q000378:metabolism; D051381:Rats; D013347:Subcellular Fractions; D000637:Transaminases / Q000302:isolation & purification*; D014364:Tryptophan / Q000378:metabolism
chemical_list: D006882:Hydroxyapatites; D007656:Ketoglutaric Acids; D011773:Pyruvates; D007737:Kynurenine; D014364:Tryptophan; D000429:Alcohol Oxidoreductases; D008995:Monoamine Oxidase; D000637:Transaminases