Difference in kinetic properties between hexokinase type I isoenzymes from various rat tissues with reference to the effect of a thiol inhibitor.
Hexokinase isoenzyme type I was purified from various rat tissues, and was subjected to kinetic studies in the presence or the absence of p-chloromercuribenzenesulphonate. The mode of the inhibition by the thiol inhibitor was different for the type I isoenzymes obtained from different tissues, suggesting that the type I isoenzymes from different tissues were not identical proteins.
D000255:Adenosine Triphosphate; D000818:Animals; D001557:Benzenesulfonates / Q000494:pharmacology; D001665:Binding Sites; D001921:Brain / Q000201:enzymology; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006593:Hexokinase / Q000037:antagonists & inhibitors*; D007527:Isoenzymes / Q000037:antagonists & inhibitors*; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008274:Magnesium; D008297:Male; D008628:Mercury / Q000494:pharmacology*; D009132:Muscles / Q000201:enzymology; D009928:Organ Specificity; D009942:Organometallic Compounds / Q000494:pharmacology*; D011485:Protein Binding; D051381:Rats; D013056:Spectrophotometry, Ultraviolet; D013154:Spleen / Q000201:enzymology; D013439:Sulfhydryl Reagents / Q000494:pharmacology*
D001557:Benzenesulfonates; D007527:Isoenzymes; D009942:Organometallic Compounds; D013439:Sulfhydryl Reagents; D000255:Adenosine Triphosphate; D006593:Hexokinase; D008628:Mercury; D008274:Magnesium