Isolation and characterization of mitochondrial deoxyribonucleic acid of Acanthamoeba castellanii.
DNA was prepared from isolated mitochondria of Acanthamoeba castellanii and was shown to behave as a single component in density gradients, on ;melting' and on renaturation. From measurements of renaturation kinetics, sedimentation coefficient and electron micrographs the genome size of the mitochondrial DNA was calculated to be about 3.4x10(7) daltons. A small proportion of the preparations could be isolated as relaxed circular molecules of mean contour length 16.2mum.
D002498:Centrifugation; D002499:Centrifugation, Density Gradient; D002845:Chromatography; D004272:DNA, Mitochondrial / Q000302:isolation & purification*; D056890:Eukaryota / Q000032:analysis*; D005838:Genotype; D006882:Hydroxyapatites; D007700:Kinetics; D008854:Microscopy, Electron; D009691:Nucleic Acid Denaturation; D009695:Nucleic Acid Renaturation; D013696:Temperature
D004272:DNA, Mitochondrial; D006882:Hydroxyapatites