Partial purification and properties of D-glucosamine 6'-phosphate N-acetyltransferase from zoospores of Blastocladiella emersonii.
A d-glucosamine 6-phosphate N-acetyltransferase from Blastocladiella emersonii zoospores was partially purified to a specific activity of 2.41 IU per mg of protein. Its pH optimum was 8.05 and its K(m) values were 2.4 x 10(-4) M d-glucosamine 6-phosphate and 0.38 x 10(-4) M Na(3)S-acetyl coenzyme A.
D000123:Acetyltransferases / Q000302:isolation & purification*; D001754:Blastocladiella / Q000201:enzymology*; D002848:Chromatography, DEAE-Cellulose; D003065:Coenzyme A / Q000378:metabolism; D005656:Fungal Proteins / Q000096:biosynthesis; D005658:Fungi / Q000201:enzymology*; D005944:Glucosamine / Q000096:biosynthesis; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010710:Phosphates / Q000378:metabolism; D013172:Spores, Fungal / Q000201:enzymology*
D005656:Fungal Proteins; D010710:Phosphates; D000123:Acetyltransferases; D005944:Glucosamine; D003065:Coenzyme A