Plasma alkaline phosphatase isoenzymes in hepatobiliary disease.
BY CELLULOSE ACETATE OR ACRYLAMIDE GEL ELECTROPHORESIS IT IS POSSIBLE TO SEPARATE THESE ALKALINE PHOSPHATASE ISOENZYMES FROM SERUM: [anode] fast liver, slow liver, placenta/Regan, bone, intestine, bile [cathode]. Heat or chemical inhibition can confirm the differentiation. Normal adult serum always contains slow-liver isoenzyme, and sometimes bone isoenzyme: the latter is always present in serum of children. In hepatobiliary disease slow-liver isoenzyme was always increased: intestinal isoenzyme appeared in many cases of cirrhosis (of blood groups B and 0) but fast-liver and bile isoenzymes were occasionally seen in miscellaneous cases. The findings in other diseases included Regan isoenzyme in six out of 45 cases of malignant disease.
D000469:Alkaline Phosphatase / Q000097:blood / Q000302:isolation & purification*; D001660:Biliary Tract Diseases / Q000201:enzymology*; D001789:Blood Group Antigens; D001842:Bone and Bones / Q000201:enzymology; D002648:Child; D004796:Clinical Enzyme Tests; D004586:Electrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007422:Intestines / Q000201:enzymology; D007527:Isoenzymes / Q000097:blood / Q000302:isolation & purification*; D007674:Kidney Diseases / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008103:Liver Cirrhosis / Q000201:enzymology; D008107:Liver Diseases / Q000201:enzymology*; D009369:Neoplasms / Q000201:enzymology; D010920:Placenta / Q000201:enzymology
D001789:Blood Group Antigens; D007527:Isoenzymes; D000469:Alkaline Phosphatase