title: Studies on plasmid replication. IV. Complementation of replication-defective mutants by an incompatibility-deficient plasmid.
abstract: This paper described a complementation test system for replication-defective S. aureus pencillinase plasmids in which the incompatibility barrier has been overcome by the isolation of an incompatibility-defective (Inc-) plasmid. This plasmid appears to be stably and irreversibly integrated into the host chromosome as attempts to restore it to its original independent state have been unsuccessful. The Inc- plasmid was able to complement the thermosensitive replication defects  of Seg- plasmids belonging to the same original incompatibility class but was unable to complement onels belonging to a different incompatibility class. Positive and negative phenotypic complementation tests were confirmed at the molecular level by isotopec labeling of plasmid-specific DNA molecules.
mesh_terms: D004261:DNA Replication; D004269:DNA, Bacterial; D005111:Extrachromosomal Inheritance; D005827:Genetics, Microbial; D009154:Mutation; D010405:Penicillinase / Q000494:pharmacology; D010641:Phenotype; D013210:Staphylococcus / Q000187:drug effects / Q000254:growth & development*
chemical_list: D004269:DNA, Bacterial; D010405:Penicillinase