A plasma protein fractionation procedure for use in studies of protein metabolism.
A two-step method for the separation of five different plasma proteins on a preparative scale, which is capable of being extended to allow the separation of other plasma proteins, is described. The proteins separated were fibrinogen, two alpha(1)-glycoproteins, albumin and transferrin. The alpha(1)-glycoproteins were characterized in terms of electrophoretic mobility, ultracentrifugal and immunological characteristics. By using this method, it was shown that a single sample of plasma could be fractionated to yield purified proteins in sufficient quantity to simultaneously measure the synthesis of the two alpha(1)-glycoproteins, albumin and transferrin in the rat with McFarlane's technique (McFarlane, 1963; Reeve et al., 1963; McFarlane et al., 1965).
D000818:Animals; D001120:Arginine / Q000097:blood; D001798:Blood Proteins / Q000302:isolation & purification*; D002254:Carbonates / Q000378:metabolism; D005591:Chemical Fractionation / Q000379:methods; D002850:Chromatography, Gel; D005227:Fatty Acids / Q000032:analysis; D005340:Fibrinogen / Q000302:isolation & purification; D005590:Fractional Precipitation; D006023:Glycoproteins / Q000032:analysis / Q000096:biosynthesis / Q000097:blood*; D006595:Hexosamines / Q000032:analysis; D006601:Hexoses / Q000032:analysis; D008259:Macroglobulins / Q000032:analysis; D008970:Molecular Weight; D010758:Phosphorus / Q000032:analysis; D011506:Proteins / Q000032:analysis; D051381:Rats; D012709:Serum Albumin / Q000302:isolation & purification; D014168:Transferrin / Q000302:isolation & purification; D014508:Urea / Q000097:blood
D001798:Blood Proteins; D002254:Carbonates; D005227:Fatty Acids; D006023:Glycoproteins; D006595:Hexosamines; D006601:Hexoses; D008259:Macroglobulins; D011506:Proteins; D012709:Serum Albumin; D014168:Transferrin; D010758:Phosphorus; D014508:Urea; D005340:Fibrinogen; D001120:Arginine