Studies on the fine structural localization of zinc-iodide-osmium reaction in the brain. II. Some characteristics of localization in certain identified synapses.
Samples of the olfactory bulb and cerebellar cortex from rats were impregnated in zinc-iodide-osmium (ZIO) solution. The fine structural localization of ZIO impregnation product was studied and, in addition, the percentage distribution of ZIO-positive synaptic vesicles was ascertained in certain synapses of known function. Spherical vesicles, particularly in aldehyde-prefixed material, were found to be more resistant to ZIO impregnation than ovoid vesicles, both in dendro-dendritic and in axo-dendritic synapses. The different ZIO reactivity of synaptic vesicles in excitatory and inhibitory synapses, apart from reflecting an important difference in the chemical composition of synaptic vesicles, may offer a new method for characterizing synapses in relation to certain functions.
D000818:Animals; D001369:Axons / Q000648:ultrastructure; D002525:Cerebellar Cortex / Q000032:analysis* / Q000648:ultrastructure; D003712:Dendrites / Q000032:analysis / Q000648:ultrastructure; D005260:Female; D007454:Iodides / Q000032:analysis*; D008297:Male; D009431:Neural Conduction; D009433:Neural Inhibition; D009830:Olfactory Bulb / Q000032:analysis* / Q000648:ultrastructure; D009992:Osmium / Q000032:analysis*; D051381:Rats; D013569:Synapses / Q000032:analysis*; D013572:Synaptic Vesicles / Q000032:analysis / Q000378:metabolism / Q000648:ultrastructure; D015032:Zinc / Q000032:analysis*
D007454:Iodides; D009992:Osmium; D015032:Zinc