A comparison of glucoside formation by liver preparations from the rabbit and the mouse.
Liver homogenates from mice and from rabbits transfer glucose from UDP-[6-(3)H]glucose, at pH7.0, to oestradiol-17alpha, oestradiol-17beta, oestradiol-17alpha 3-glucuronide, p-nitrophenol and diethylstilboestrol. In the rabbit the phenolic steroids were better substrates than p-nitrophenol for the glucosyltransferase, whereas the reverse was true in the mouse. At pH8.0, rabbit liver, but not mouse liver, transferred glucose to oestradiol-17alpha 3-glucuronide in better yield than that at pH7.0. Evidence is presented for the presence of two glucosyltransferases in rabbit liver. One of these has a pH optimum at about 8.0, and is highly specific for oestradiol-17alpha 3-glucuronide, whereas the other, which has a pH optimum at about 7.0, is similar in this respect to the transferase in mouse liver.
D000818:Animals; D002250:Carbon Radioisotopes; D004054:Diethylstilbestrol / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D004964:Estriol / Q000378:metabolism; D005260:Female; D005964:Glucosyltransferases / Q000378:metabolism; D005965:Glucuronates / Q000378:metabolism; D006027:Glycosides / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D051379:Mice; D009596:Nitrophenols / Q000378:metabolism; D011817:Rabbits; D013739:Testosterone / Q000378:metabolism; D014316:Tritium; D014539:Uridine Diphosphate Sugars / Q000378:metabolism
D002250:Carbon Radioisotopes; D005965:Glucuronates; D006027:Glycosides; D009596:Nitrophenols; D014539:Uridine Diphosphate Sugars; D014316:Tritium; D013739:Testosterone; D004958:Estradiol; D004054:Diethylstilbestrol; D005964:Glucosyltransferases; D004964:Estriol