Protein and carbohydrate composition of the cell envelope of Halobacterium salinarium.
The isolated cell envelope of Halobacterium salinarium strain 1 contained 15 to 20 proteins that were resolved by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. All but one of these proteins had molecular weights of 130,000 or less and together accounted for 50 to 60% of the total envelope protein. The remaining 40 to 50% of the envelope protein was accounted for by a single protein with an apparent molecular weight of approximately 194,000 that stained for carbohydrate with periodate-Schiff reagent. The proteolytic enzymes trypsin and Pronase were used to show that the carbohydrate is covalently bound to the protein. Separation of amino sugar- and hexose-containing tryptic peptides by gel filtration indicated that all of the nonlipid carbohydrate of the cell envelope is covalently bound to protein. The results of partial purification by phenol extraction indicated that both the amino sugar and hexose are bound to the 194,000-molecular-weight protein. Exposure of isolated cell envelopes to low salt concentration resulted in solubilization of a majority of the envelope proteins. A relatively small number of proteins, including the high-molecular-weight, carbohydrate-containing protein, remained bound to the sedimentable cell membrane fraction.
D001426:Bacterial Proteins / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis; D002850:Chromatography, Gel; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D006217:Halobacterium / Q000032:analysis* / Q000166:cytology; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000032:analysis; D008623:Mercaptoethanol; D008970:Molecular Weight; D010447:Peptide Hydrolases; D010636:Phenols; D012967:Sodium Dodecyl Sulfate
D001426:Bacterial Proteins; D002241:Carbohydrates; D003470:Culture Media; D008070:Lipopolysaccharides; D010636:Phenols; D012967:Sodium Dodecyl Sulfate; D008623:Mercaptoethanol; D010447:Peptide Hydrolases