Studies on glucarate catabolism: the oxodeoxyglucarate aldolase activity of glucarate hydro-lyase from Pseudomonas acidovorans.
Glucarate hydro-lyase was isolated and purified to near homogeneity from cells of Pseudomonas acidovorans grown on glucarate. By using gel filtration and ion-exchange chromatography, it was shown that the oxodeoxyglucarate aldolase activity observed in such extracts is associated with the glucarate hydro-lyase protein.
D000272:Adipates / Q000031:analogs & derivatives*; D000446:Aldehyde-Lyases / Q000378:metabolism*; D002498:Centrifugation; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006836:Hydro-Lyases / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D011549:Pseudomonas / Q000201:enzymology*; D013400:Sugar Acids; D013646:Tartronates
D000272:Adipates; D013400:Sugar Acids; D013646:Tartronates; D000446:Aldehyde-Lyases; D006836:Hydro-Lyases