Pathways of glyceride glycerol synthesis.
Isolated rat liver parenchymal cells were incubated for various periods with [U-(14)C,2-(3)H]glycerol and the radioisotopic yields in the major products were determined, as well as the (3)H/(14)C ratios in glyceride glycerol and intracellular glycerol phosphate. Under the conditions used (0.1mm-glycerol+10mm-l-lactate or 10mm-glycerol as substrates), only small differences were found between these (3)H/(14)C ratios. The results suggest a minor role for a pathway of glyceride glycerol synthesis involving reduction of acylated dihydroxyacetone phosphate, under these experimental conditions.
D000096:Acetone / Q000378:metabolism; D000135:Acid Phosphatase; D000818:Animals; D002250:Carbon Radioisotopes; D002498:Centrifugation; D005989:Glycerides / Q000096:biosynthesis*; D005990:Glycerol / Q000096:biosynthesis* / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D008297:Male; D009943:Organophosphorus Compounds / Q000378:metabolism; D051381:Rats; D014306:Trioses / Q000378:metabolism; D014316:Tritium
D002250:Carbon Radioisotopes; D005989:Glycerides; D009943:Organophosphorus Compounds; D014306:Trioses; D014316:Tritium; D000096:Acetone; D000135:Acid Phosphatase; D005990:Glycerol