Studies on the subunit structure and amino acid sequence of trisoe phosphate isomerase from chicken breast muscle.
1. Triose phosphate isomerase was prepared by chromatography on DEAE-cellulose of an (NH(4))(2)SO(4) fraction of an extract of homogenized chicken breast muscle. The product is homogeneous on gel electrophoresis and is suitable for growing crystals for X-ray work. The specific activity is 10000 units/mg and the value for E(0.1%) (280) is 1.20. 2. Comparison between the sum of the amino acid compositions of the tryptic peptides of the protein and the amino acid composition obtained on total hydrolysis of the protein indicates that the relative subunit mass is about 27000. 3. These data, together with the results of the examination of the amino acid compositions of a number of minor peptides, the number of peptides in the tryptic digest and the complete amino acid sequences of the tryptic peptides (the determination of which is described here), give no indication that the subunits are dissimilar. 4. A tentative amino acid sequence is presented for the protein, in which the ordering of the tryptic peptides is derived by homology with the sequence of the rabbit muscle enzyme (Corran & Waley, 1973). 5. An appendix describes the use that was made of mass spectrometry in the determination of some of the sequences. Mass-spectrometric data have been obtained for 35 residues, that is about 15% of the total sequence of the protein. 6. An extended version of the present paper has been deposited as Supplementary Publication SUP 50025 at the British Library, Lending Division (formerly the National Lending Library for Science and Technology), Boston Spa, Yorks. LS23 7BQ, U.K., from whom copies may be obtained on the terms given in Biochem. J. (1973) 131, 5.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002238:Carbohydrate Epimerases; D002268:Carboxypeptidases; D002645:Chickens; D002848:Chromatography, DEAE-Cellulose; D002854:Chromatography, Paper; D003460:Crystallization; D004590:Electrophoresis, Paper; D004591:Electrophoresis, Polyacrylamide Gel; D007461:Iodoacetates; D046911:Macromolecular Substances; D008970:Molecular Weight; D009132:Muscles / Q000201:enzymology*; D010446:Peptide Fragments / Q000032:analysis; D011817:Rabbits; D013045:Species Specificity; D013820:Thermolysin; D014305:Triose-Phosphate Isomerase / Q000302:isolation & purification; D014357:Trypsin; D014961:X-Ray Diffraction
D000596:Amino Acids; D007461:Iodoacetates; D046911:Macromolecular Substances; D010446:Peptide Fragments; D002268:Carboxypeptidases; D014357:Trypsin; D013820:Thermolysin; D002238:Carbohydrate Epimerases; D014305:Triose-Phosphate Isomerase