title: Glucuronic acid pathway in alloxan diabetic rabbits. (I). Urinary excretion of metabolites related to the glucuronic acid pathway.
abstract: Studies on the activity of the glucuronic acid pathway in alloxan diabetic rabbits were carried out. Amount of D-glucaric acid, L-ascorbic acid, and D-glucuronic acid in urine increased in the case of the alloxan diabetic rabbits. The transformation from D-glucuronolactone to D-glucaric acid was higher than normal in the diabetic animals. The expired 14-CO2 decreased and urinary excretion of labeled L-gulonic acid increased after administration of 6-14-C-glucuronolactone in the diabetic rabbits. L-Gulonic acid dehydrogenase, lactonase II, and beta-glucuronidase activities were reduced, and UDPGA-pyrophosphatase, D-glucuronic acid-1-phosphatase, and UDPGA-transferase activities increased in the diabetic rabbit liver. From these results, it may be concluded that an increase of endogenous D-glucuronic acid in the diabetic states could be attributed to a metabolid defect in the step of L-gulonic acid dehydrogenation and to the enhancement of UDPGA-pyrophosphatase and D-glucuronic acid-1-phosphate phosphatase activities.
mesh_terms: D000272:Adipates / Q000652:urine; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000652:urine; D002250:Carbon Radioisotopes; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000652:urine; D005965:Glucuronates / Q000378:metabolism* / Q000652:urine; D007783:Lactones / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008297:Male; D011817:Rabbits; D013400:Sugar Acids / Q000652:urine
chemical_list: D000272:Adipates; D002250:Carbon Radioisotopes; D005965:Glucuronates; D007783:Lactones; D013400:Sugar Acids; D000429:Alcohol Oxidoreductases; D001205:Ascorbic Acid