Thiol-blocking reagents and phosphate acetyltransferase catalysis, and the assessment of protection by adsorbed molecules.
Phosphate acetyltransferase (EC 2.3.1.8) is shown to be unusually sensitive to thiol-blocking reagents, and one or more thiol groups probably catalyse the surface reaction. A novel method for assessing protection by adsorbed species is described. The adsorbed substrate molecules acetyl-CoA and phosphate are found to provide substantial protection against thiol-blocking reagents.
D000081:Acetamides / Q000494:pharmacology; D000105:Acetyl Coenzyme A; D000123:Acetyltransferases / Q000037:antagonists & inhibitors*; D000327:Adsorption; D001565:Benzoates / Q000494:pharmacology; D002384:Catalysis; D007461:Iodoacetates / Q000494:pharmacology; D008626:Mercuribenzoates / Q000494:pharmacology; D013439:Sulfhydryl Reagents / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D000081:Acetamides; D001565:Benzoates; D007461:Iodoacetates; D008626:Mercuribenzoates; D013439:Sulfhydryl Reagents; D000105:Acetyl Coenzyme A; D000123:Acetyltransferases