Effect of D-glucaric acid derivatives on stability of rat liver lysosomes and erythrocytes.
For studies on the lysosome-stabilizing effect of D-glucaric acid derivatives which have been found to have anti-inflammatory effect, the available and soluble enzyme activities of acid phosphatase of rat liver lysosomes were determined. Saliployed as standards. Lysosomes were incubated with drugs under specific conditions which allowed the data on the stabilizing activity of the drugs to be reproducible. The inhibitory effect of D-glucaro-1, 4-lactone, salicylic acid and phenylbutazone onover a wide range of concentrations. D-glucaro-1, 4-lactone as well as salicylic acid exhibited concentration-dependent lysosome-stabilizing effect whereas phenlybutazone had an optimum concentration for its lysosome-stabilizing effect. In addition, D-glucaro-1, 4-lactone,saliclicacid and phenylubtazone were also examined for their effects on heat-induced and saponin-induced hemolysis of rat erythrocyres. Both salicylic acid and phenylbutazone exhibited potent stabilziing and labilizing effects on heat-induced and saponin-induced hemolysis of eryhthrocytes, respectively. D-glucaro-1, 4-lactone, however, was incabale of affecting the hemolysis of erythrocytes. There appears to be a difference in the mechanism of the lysosome-stabilizing effects between D-glucaric acid derivatives and other anti-inflammatory drugs.
D000135:Acid Phosphatase / Q000378:metabolism; D000272:Adipates / Q000494:pharmacology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects*; D000431:Ethanol / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008247:Lysosomes / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D009005:Monosaccharides / Q000494:pharmacology; D010653:Phenylbutazone / Q000494:pharmacology; D051381:Rats; D012459:Salicylates / Q000494:pharmacology; D012503:Saponins / Q000494:pharmacology; D013395:Sucrose / Q000378:metabolism; D013400:Sugar Acids / Q000494:pharmacology
D000272:Adipates; D000893:Anti-Inflammatory Agents; D009005:Monosaccharides; D012459:Salicylates; D012503:Saponins; D013400:Sugar Acids; D000431:Ethanol; D013395:Sucrose; D000135:Acid Phosphatase; D010653:Phenylbutazone