title: Evidence for two waves of induction of DNA enzymes in stimulated human lymphocytes.
abstract: The stimulation of human lymphocytes with phytohaemoagglutinin induces the appearance or increase of several enzymes of DNA metabolism [Pedrini etal., Biochem. Biophys. Res. Comm., 47:1221(1972)]. With long times of stimulation, two phenomena are observed; an increase in the levels of DNA polymerase, of a DNase acting on single-stranded DNA, and of an endonuclease, occurring between the third and fourth day, in parallel with a wave of DNA synthesis;a second wave of increase of the same enzymes and of DNA ligase,occurring between the fifth and eight day when the DNA replication rate, as measured by thymidine-pulses, has decreased to values close to the background.
mesh_terms: D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism; D006801:Humans; D037102:Lectins / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D011118:Polynucleotide Ligases / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
chemical_list: D037102:Lectins; D004247:DNA; D004254:DNA Nucleotidyltransferases; D003851:Deoxyribonucleases; D004720:Endonucleases; D011118:Polynucleotide Ligases; D013936:Thymidine