Purification of alpha-mannosidase from jack-bean meal suitable for use as reagent.
A simple method of purification of alpha-mannosidase from jack-bean meal is described which yields a product free of beta-N-acetylglucosaminidase activity.
D002498:Centrifugation; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003956:Dialysis; D006026:Glycoside Hydrolases / Q000302:isolation & purification*; D007202:Indicators and Reagents; D008358:Mannose; D008722:Methods; D010944:Plants / Q000201:enzymology*
D007202:Indicators and Reagents; D006026:Glycoside Hydrolases; D008358:Mannose