title: Peroxidatic oxidation of tyrosine to melanin in supernatant of crude mouse melanoma homogenates.
abstract: Peroxide-dependent enzymic oxidation of tyrosine to dopachrome and melanin was demonstrated in cell-free melanoma homogenates. Histochemical methods for distinguishing peroxidase activity from aerobic dopa (3,4-dihydroxyphenylalanine) oxidase activity are not reliable with cell-free preparations. Therefore the presence of peroxidase activity in such preparations precludes assay of cresolase activity of mammalian ;tyrosinase'.
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D008543:Melanins / Q000096:biosynthesis*; D008545:Melanoma / Q000378:metabolism*; D051379:Mice; D010084:Oxidation-Reduction; D010545:Peroxides / Q000378:metabolism; D013053:Spectrophotometry; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D008543:Melanins; D010545:Peroxides; D014443:Tyrosine