title: Isolation of microvillus plasma membranes from suckling-rat intestine. The influence of premature induction of digestive enzymes by injection of cortisol acetate.
abstract: 1. Cortisone administration to suckling rats leads prematurely to induction of enzymes of the intestinal microvillus plasma membrane and lengthening of the intestinal microvilli. To investigate the membrane changes that might be involved, a method for the isolation of a fraction enriched with microvillus plasma membrane was developed in suckling rats. Plasma-membrane fractions were compared from 13-day-old control rats and from 13-day-old rats given cortisol acetate by subcutaneous injection for 3 days. 2. After cortisol injection, the activity of maltase, trehalase, sucrase and leucyl beta-naphthylamidase increased markedly, and to the same extent, in intestinal homogenates and plasma-membrane preparations. Purification, and recovery of five marker enzymes with respect to homogenate activity, and recovery of protein, were similar for both membrane preparations, particularly after correction for non-membrane activity, which was high in suckling rats and affected by cortisol. 3. In material released from the plasma membrane by digestion with papain, maltase protein was increased after cortisol injection at least as much as maltase activity. Sucrase activity increased at least 200-fold, and this increase was associated with the appearance of a new sucrase band on polyacrylamide-gel electrophoresis. 4. Sodium dodecyl sulphate electrophoresis of plasma-membrane proteins revealed at least four additional macromolecules after cortisol injection. Concurrently several proteins disappeared from the plasma membrane. The added proteins appeared in the main to be removed from the plasma membrane by papain, whereas the deleted proteins were in the papain-resistant fraction. 5. Enzymic stimulation induced by cortisol acetate in the suckling-rat plasma membrane therefore appears to involve the addition of new proteins, rather than activation of proteins in situ. Deletion of proteins from the membrane during induction of hydrolytic enzymes may reflect other phenomena such as protein reorganization associated with the change in microvillus shape.
mesh_terms: D000626:Aminopeptidases / Q000378:metabolism; D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000032:analysis / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction; D005959:Glucosidases / Q000378:metabolism; D006854:Hydrocortisone / Q000008:administration & dosage / Q000494:pharmacology*; D006868:Hydrolysis; D007279:Injections, Subcutaneous; D007422:Intestines / Q000201:enzymology*; D010206:Papain; D011506:Proteins / Q000032:analysis; D051381:Rats; D013393:Sucrase / Q000378:metabolism; D014198:Trehalase / Q000378:metabolism
chemical_list: D011506:Proteins; D005959:Glucosidases; D014198:Trehalase; D013393:Sucrase; D000626:Aminopeptidases; D010206:Papain; D006854:Hydrocortisone