Studies on the fractionation of total transfer ribonucleic acid and purification of an alanine transfer ribonucleic acid from chick embryo.
Chick embryo tRNA, prepared by a simple large-scale method, was fractionated on three different ion-exchange columns. In all cases simple chromatographic patterns for various tRNA species were observed, indicating the presence of only a few major species of tRNA for each amino acid. By repeated chromatography one species of alanine tRNA was purified to approx. 80% purity. T(1) ribonuclease digest of this purified tRNA gave a simple chromatographic pattern. Because of the simplicity of the method of preparation of tRNA from this readily available source and the presence of only a few species of tRNA for each amino acid, chick embryo is suited for the study of tRNA and its various functions in higher systems.
D000409:Alanine / Q000378:metabolism; D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D005591:Chemical Fractionation; D002642:Chick Embryo; D002852:Chromatography, Ion Exchange; D006639:Histidine / Q000378:metabolism; D007532:Isoleucine / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D012343:RNA, Transfer / Q000302:isolation & purification*; D012260:Ribonucleases / Q000378:metabolism; D012694:Serine / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism; D014633:Valine / Q000378:metabolism
D007532:Isoleucine; D001224:Aspartic Acid; D014443:Tyrosine; D012694:Serine; D006639:Histidine; D012343:RNA, Transfer; D011392:Proline; D012260:Ribonucleases; D014633:Valine; D008239:Lysine; D000409:Alanine