Protein synthesis by synaptosomes from rat brain. Contribution by the intraterminal mitochondria.
(1) The characteristics of protein synthesis in microsomal and synaptosomal fractions from rat brain were examined. A high sensitivity to ribonuclease and to cycloheximide, and the need for the presence of pH5 enzymes distinguished protein synthesis in microsomal fractions from protein synthesis in synaptosomes. (2) Under various conditions of incubation synaptosomal fractions prepared in sucrose showed limited protein synthesis compared with synaptosomal fractions prepared by using Ficoll. Such discrepancies could not be attributed to: (i) animal age, (ii) the metabolic state of the synaptosomal fraction, (iii) the absence of bivalent cations in the incubation medium or (iv) the temperature. (3) Protein synthesis in synaptosomal fractions was inhibited 50-65% by cycloheximide, 38-50% by chloramphenicol, 95% by puromycin, 70% by azide and 40% by deoxyglucose; ribonuclease had only a negligible inhibitory effect. (4) As a first approximation to the localization of the protein-synthetic machinery present in the synaptosomal fraction, the distribution of enzymes and radioactivity in subfractions of prelabelled synaptosomes was determined after osmotic shock with water. Approximately 60% of the total protein synthesis in the synaptosomal fraction occurred in the intraterminal mitochondria. (5) Protein synthesis in the intraterminal mitochondria did not show any fundamental difference from synthesis in somatic mitochondria, with respect to inhibition by cycloheximide and chloramphenicol. (6) It was concluded that if extramitochondrial protein synthesis occurs in synaptosomes, it must be very low.
D000367:Age Factors; D000818:Animals; D001386:Azides / Q000494:pharmacology; D001921:Brain / Q000378:metabolism*; D002118:Calcium; D002250:Carbon Radioisotopes; D002498:Centrifugation; D002701:Chloramphenicol / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D003837:Deoxy Sugars; D005947:Glucose; D066298:In Vitro Techniques; D008274:Magnesium; D008297:Male; D008345:Manganese; D008861:Microsomes / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D009995:Osmosis; D014176:Protein Biosynthesis; D011691:Puromycin / Q000494:pharmacology; D051381:Rats; D012260:Ribonucleases; D013395:Sucrose; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*; D013696:Temperature; D014316:Tritium
D001386:Azides; D002250:Carbon Radioisotopes; D003837:Deoxy Sugars; D014316:Tritium; D008345:Manganese; D011691:Puromycin; D013395:Sucrose; D002701:Chloramphenicol; D003513:Cycloheximide; D012260:Ribonucleases; D008274:Magnesium; D005947:Glucose; D002118:Calcium