An improved micro-method for avidin assay.
A sensitive method for avidin assay was devised. Tritiated biotin is bound to avidin and this complex is then bound to bentonite. Radioactivity is converted into a gas form by combustion and counted in an automatic proportional counter with 55% efficiency and background of 3.7c.p.m. The sensitivity is 1-2ng of avidin.
D000818:Animals; D001360:Avidin / Q000032:analysis*; D001665:Binding Sites; D001710:Biotin; D002645:Chickens; D005260:Female; D008722:Methods; D008832:Microchemistry; D010047:Ovalbumin / Q000031:analogs & derivatives*; D010057:Oviducts / Q000032:analysis; D014316:Tritium
D014316:Tritium; D001360:Avidin; D001710:Biotin; D010047:Ovalbumin