Radioimmunoassay for human procollagen.
Rabbit antiserums were produced against the procollagen molecule secreted into the medium of cultured human skin fibroblasts. The isolated antigenic, amino terminal portion of the procollagen molecule was purified, labeled with iodine-125, and used in a radioimmunoassay which detected nanogram quantities of the same antigen. With the assay, immunologically identical molecules were detected in the culture mediumn of different strains of human fibroblasts and in normal human serums. Serumns from human cord blood contained a 12-fold higher concentration of the antigen than serums from adults, while serums other vertebrates gave reactions to incomplete cross-reactivity or non-reactivity.
D000818:Animals; D002417:Cattle; D002460:Cell Line; D002645:Chickens; D003094:Collagen / Q000032:analysis* / Q000097:blood; D003429:Cross Reactions; D006801:Humans; D051379:Mice; D011498:Protein Precursors / Q000032:analysis* / Q000097:blood; D011863:Radioimmunoassay; D012756:Sheep; D013045:Species Specificity
D011498:Protein Precursors; D003094:Collagen