Absence of circular plasmid deoxyribonucleic acid attributable to a genetic determinant for methicillin resistance in Staphylococcus aureus.
Plasmid deoxyribonucleic acid was not detected by centrifugal analysis of lysates of penicillinase-negative strains of Staphylococcus aureus harboring a determinant of methicillin resistance derived from strain Villaluz. When these strains contained a penicillinase plasmid, the plasmid deoxyribonucleic acid of methicillin-resistant and methicillin-susceptible strains was indistinguishable by the methods employed. The results indicate that the genetic determinant for methicillin resistance in the strains examined was not associated with a circular plasmid comparable to those that have been shown to determine resistance to benzylpenicillin, tetracycline, and chloramphenicol in S. aureus.
D002499:Centrifugation, Density Gradient; D002586:Cesium; D004269:DNA, Bacterial / Q000032:analysis*; D004270:DNA, Circular / Q000032:analysis*; D003956:Dialysis; D004996:Ethidium; D005111:Extrachromosomal Inheritance; D005815:Genetic Code; D008712:Methicillin / Q000494:pharmacology*; D008970:Molecular Weight; D010403:Penicillin Resistance; D013210:Staphylococcus / Q000032:analysis* / Q000187:drug effects*; D013395:Sucrose; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D004269:DNA, Bacterial; D004270:DNA, Circular; D014316:Tritium; D002586:Cesium; D013395:Sucrose; D004996:Ethidium; D008712:Methicillin; D013936:Thymidine