Transport of catecholamines by resealed chromaffin-grnaule "ghosts".
A method is described for the preparation of resealed chromaffin-granule ;ghosts'. The lysis and rapid purification procedures provide ;ghosts' in approximately 70% yield from crude granules; the preparation contains 0.1mumol of catecholamine/mg of protein (as compared with 2.8mumol/mg in unlysed granules), of which about one third is inside the ;ghosts'. The ;ghosts' retain their ability to accumulate catecholamines, a process dependent on Mg-ATP and inhibited by reserpine, and a simple assay for this transport is described.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000313:Adrenal Medulla / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002395:Catecholamines / Q000032:analysis / Q000378:metabolism*; D002417:Cattle; D002499:Centrifugation, Density Gradient; D002838:Chromaffin System / Q000378:metabolism* / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000378:metabolism* / Q000648:ultrastructure; D008274:Magnesium / Q000378:metabolism; D008722:Methods; D008854:Microscopy, Electron; D012110:Reserpine / Q000494:pharmacology
D002395:Catecholamines; D012110:Reserpine; D000255:Adenosine Triphosphate; D008274:Magnesium