Calf lens crystallin messenger RNA's contain polynucleotide sequences rich in adenylic acid.
Calf lens messenger fractions have been purified on poly(dT)-cellulose. The purified RNA's have been analyzed as nucleosides by a method which can be used on a micro-scale with highly reproduceble results. Both the 10S and 14S lens mRNA contain poly A sequences. The poly A content found in 14S mRNA cannot account for the excess nucleotides in this message.
D000249:Adenosine Monophosphate; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002482:Cellulose; D002499:Centrifugation, Density Gradient; D002501:Centrifugation, Zonal; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003459:Crystallins; D007908:Lens, Crystalline / Q000032:analysis / Q000166:cytology; D011119:Polynucleotides; D011132:Polyribosomes / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis / Q000302:isolation & purification; D012265:Ribonucleotides / Q000032:analysis; D012967:Sodium Dodecyl Sulfate; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013936:Thymidine; D013997:Time Factors
D003459:Crystallins; D011119:Polynucleotides; D012333:RNA, Messenger; D012265:Ribonucleotides; D012967:Sodium Dodecyl Sulfate; D000249:Adenosine Monophosphate; D002482:Cellulose; D013936:Thymidine