A rapid procedure for derivatizing agarose with a variety of carbohydrates: its use for affinity chromatography of lectins.
D000596:Amino Acids / Q000032:analysis; D001665:Binding Sites; D002241:Carbohydrates; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D005688:Galactosamine / Q000031:analogs & derivatives; D005690:Galactose; D005944:Glucosamine / Q000031:analogs & derivatives; D005947:Glucose / Q000031:analogs & derivatives; D006027:Glycosides; D007700:Kinetics; D007785:Lactose / Q000031:analogs & derivatives; D037102:Lectins / Q000302:isolation & purification*; D008358:Mannose; D008722:Methods; D009596:Nitrophenols; D037121:Plant Lectins; D011134:Polysaccharides; D011485:Protein Binding; D013025:Glycine max; D013056:Spectrophotometry, Ultraviolet
D000596:Amino Acids; D002241:Carbohydrates; D006027:Glycosides; D037102:Lectins; D009596:Nitrophenols; D037121:Plant Lectins; D011134:Polysaccharides; D005688:Galactosamine; D005947:Glucose; D007785:Lactose; D005944:Glucosamine; D008358:Mannose; D005690:Galactose