[The distribution of nuclear and cytoplasmic proteins in the blastoderm of the loach, Misgurnus fossilis L].
The concentration of dry substance (protein) and the dry weight of nuclei, cytoplasm and cells from different blastoderm regions at the early blastula and midgastrula stages were determined by interferentional microscopy. It was shown that at the early blastula stage the dry weight of cells in the basal layer is higher than that in the outer layer. Although the protein concentration in the basal layer cells appears to be somewhat higher, differences in their dry weight are due primarily to the big volume of cytoplasm of the basal layer cells. By the midgastrula stage, the total (nucleus + cytoplasm) protein concentration increases (by 17% in the basal layer cells and by 9% in the outer layer cells) due to the increase of nuclear protein concentration. At the same time dry weight of these cells markedly decreases due to the decrease of their volumes in the process of cell divisions. At the midgastrula stage the epiblast cells have the highest dry weight due to the highest protein concentration in the cytoplasm and the biggest cell volume. The results obtained are discussed with respect to the data on the pattern of accumulation of newly synthesized protein in nuclei and cytoplasm with special reference to the duration of individual cell cycle phases.
D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D001756:Blastoderm / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D005399:Fishes / Q000378:metabolism*; D006651:Histocytochemistry; D008857:Microscopy, Interference; D009698:Nucleoproteins / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*
D009698:Nucleoproteins; D011506:Proteins
Raspredelenie iadernykh i tsitoplazmaticheskikh belkov v blastoderme v'iuna Misgurnus fossilis L