Ehrlich ascites tumor preservation for fifteen years--a simple method.
The Ehrlich ascites tumor was preserved for 15 years by adding to it an equal volume of 40% glycerol and placing the preparation directly into a freezer at -60 C.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor; D002452:Cell Count; D046508:Culture Techniques; D005615:Freezing; D005990:Glycerol; D011309:Preservation, Biological / Q000379:methods*; D013997:Time Factors
D005990:Glycerol