The effects of two disulphides on cholinesterase activity in the spectrophotometric assay.
1. 5,5'-Dithiobis-(2-nitrobenzoate) did not influence serum cholinesterase activity, whereas 2,2'-dithiodipyridine had an inhibitory effect. 2. The lowering of the molar extinction coefficients observed in the presence of physostigmine may be a result of a reaction between thiolate ions with carbamate moieties. 3. The use of 5,5'-dithiobis-(2-nitrobenzoate) is still recommended in investigations, especially where the quantitative aspects are significant.
D000110:Acetylcholinesterase / Q000097:blood*; D000818:Animals; D002091:Butyrylcholinesterase / Q000097:blood; D002802:Cholinesterases / Q000097:blood*; D004220:Disulfides / Q000494:pharmacology*; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology*; D004912:Erythrocytes / Q000201:enzymology; D006736:Horses; D007700:Kinetics; D009579:Nitrobenzoates / Q000494:pharmacology*; D010830:Physostigmine / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology*; D013997:Time Factors
D004220:Disulfides; D009579:Nitrobenzoates; D011725:Pyridines; D004228:Dithionitrobenzoic Acid; D010830:Physostigmine; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D002802:Cholinesterases