The fractionation of calf thymus DNA by multiple formation of sedimentable complexes with homologous lysine-rich histone KAP. Derivative melting curves and ultracentrifugation in CsCl concentration gradients.
The process of fractionation of total calf thymus DNA using a step precipitation of DNA by means of increasing concentrations of the homologous histone KAP was investigated. In addition to the known fractions three so far undescribed ones/in thymus/,characterized by buoyant densities in CsCl equal 1.692, 1.706 and 1.728 g/ccm, were identified. Considerable amounts of preparations seriously enriched in individual satellite fractions were obtained. The ability of GC-rich satellite DNAs to form more soluble complexes with histone KAP is suggested as reason for the observed fractionation.
D000818:Animals; D002417:Cattle; D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004247:DNA / Q000302:isolation & purification; D006657:Histones; D006358:Hot Temperature; D007700:Kinetics; D008239:Lysine; D046911:Macromolecular Substances; D008722:Methods; D009691:Nucleic Acid Denaturation; D011485:Protein Binding; D013056:Spectrophotometry, Ultraviolet; D013950:Thymus Gland / Q000032:analysis*
D006657:Histones; D046911:Macromolecular Substances; D004247:DNA; D008239:Lysine