Serological comparison of five arginine-utilizing Mycoplasma species by two-dimensional immunoelectrophoresis.
Five arginine-utilizing, nonglycolytic Mycoplasma species were compared serologically by two dimensional immunoelectrophoresis. The survey included: Mycoplasma hominis, Mycoplasma arthritidis, Mycoplasma gateae, Mycoplasma gallinarum, and three strains of Mycoplasma arginini. Although the M. arginini strains showed strong cross-reactions, each strain produced a different antigenic profile which was distinct and which indicated significant antigenic differences between strains. The M. arginini strains showed strong relationships with M. gateae; lesser cross-reactions were observed with other strains. Common antigens were demonstrable in the entire group. When all these species were compared with a glycolytic species, Mycoplasma gallisepticum, no cross-reactions were observed. Two-dimensional immunoelectrophoresis was a far more sensitive technique than double-immunodiffusion since as many as 20 components could be resolved.
D000818:Animals; D000942:Antigens, Bacterial; D001120:Arginine / Q000378:metabolism*; D003429:Cross Reactions; D007106:Immune Sera; D007122:Immunoelectrophoresis; D009174:Mycoplasma / Q000145:classification / Q000276:immunology* / Q000378:metabolism; D011817:Rabbits / Q000276:immunology; D013045:Species Specificity
D000942:Antigens, Bacterial; D007106:Immune Sera; D001120:Arginine