[Activation of mitochondrial DNA synthesis during loach embryogenesis].
Mitochondria isolated from unfertilized loach (Misgurnus fossilis) eggs incorporate 3H-dTTP at a low rate (0,01 pmoles 3H-dTTP-mg of mitochondrial protein/1 hr incubation). After fertilization the rate of 3H-dTTP incorporation into DNA of mitochondria isolated from embryos of different developmental stages increases exponentially, doubling each 7 hours, and attains the maximum to 35 hour of development. This stage corresponds to the beginning of movement. DNA synthesis in mitochondria from unfertilized eggs resembles repair; as early as 6 hours after fertilization the labeling pattern of mt-DNA is of replicative type. This replicative type of labeling is observed throughout all early development. Activation of mt-DNA biosynthesis in the course of early development is not accompanied by any changes of DNA polymerase activity in mitochondrial extracts or in mitochondrial lysates.
D000818:Animals; D004272:DNA, Mitochondrial / Q000096:biosynthesis*; D005260:Female; D005399:Fishes / Q000196:embryology* / Q000378:metabolism; D010063:Ovum / Q000378:metabolism*
D004272:DNA, Mitochondrial
Aktivatsiia biosinteza mitokhondrial'noĭ DNK v émbriogeneze v'iuna