The purification and properties of hen oviduct form B DNA-dependent RNA polymerase.
Hen oviduct form B DNA-dependent RNA polymerase has been extensively purified and its properties analysed. It seems likely to consist of a mixture of two forms of the type observed in tissues from other species. Furthermore using S1 nuclease to digest single-stranded DNA, we show that although form B can transcribe double-stranded DNA template it has a very strong preference for single-stranded regions. Also the rate of elongation on native DNA in vitro was measured and is an order of magnitude slower than that reported to be operative in vivo.
D000818:Animals; D001487:Basidiomycota; D002499:Centrifugation, Density Gradient; D002645:Chickens; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D004247:DNA; D004277:DNA, Single-Stranded; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification* / Q000378:metabolism; D003851:Deoxyribonucleases; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007700:Kinetics; D009183:Mycotoxins / Q000494:pharmacology; D009842:Oligopeptides / Q000494:pharmacology; D010057:Oviducts / Q000201:enzymology*; D010456:Peptides, Cyclic / Q000494:pharmacology; D013997:Time Factors; D014158:Transcription, Genetic; D014465:Ultrasonics
D004277:DNA, Single-Stranded; D009183:Mycotoxins; D009842:Oligopeptides; D010456:Peptides, Cyclic; D004247:DNA; D012321:DNA-Directed RNA Polymerases; D003851:Deoxyribonucleases