Reaction of pyridoxal 5'-sulfate with apoenzyme of aspartate aminotransferase. Covalent labeling of the protein with elimination of sulfate.
D000818:Animals; D001051:Apoenzymes / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000378:metabolism*; D001665:Binding Sites; D002942:Circular Dichroism; D003545:Cysteine / Q000378:metabolism; D003593:Cytoplasm / Q000201:enzymology; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D009206:Myocardium / Q000201:enzymology*; D009943:Organophosphorus Compounds / Q000378:metabolism; D010084:Oxidation-Reduction; D011485:Protein Binding; D011730:Pyridoxal / Q000378:metabolism*; D011732:Pyridoxal Phosphate / Q000378:metabolism; D011733:Pyridoxamine / Q000378:metabolism; D011736:Pyridoxine / Q000378:metabolism; D012545:Schiff Bases; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013464:Sulfuric Acids / Q000378:metabolism*; D013552:Swine
D001051:Apoenzymes; D009943:Organophosphorus Compounds; D012545:Schiff Bases; D013438:Sulfhydryl Compounds; D013464:Sulfuric Acids; D011730:Pyridoxal; D011732:Pyridoxal Phosphate; D011733:Pyridoxamine; D001219:Aspartate Aminotransferases; D003545:Cysteine; D011736:Pyridoxine