Enzymatic formation of (20R, 22R)-20,22-dihydroxycholesterol from cholesterol and a mixture of 16O2 and 18O2: random incorporation of oxygen atoms.
D000302:Adrenal Cortex / Q000378:metabolism; D000818:Animals; D002250:Carbon Radioisotopes; D002417:Cattle; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000378:metabolism*; D002849:Chromatography, Gas; D006888:Hydroxycholesterols / Q000096:biosynthesis*; D007553:Isotope Labeling; D013058:Mass Spectrometry; D008928:Mitochondria / Q000378:metabolism; D010103:Oxygen Isotopes
D002250:Carbon Radioisotopes; D006888:Hydroxycholesterols; D010103:Oxygen Isotopes; D002784:Cholesterol