The effects of arginine deficiency on lymphoma cells.
When L5178Y and L1210 mouse lymphosarcoma cells were incubated with rat or beef liver arginase there was up to 100% cell destruction in 24 hours. This was reversed specifically with arginine and partially with arginino-succinic acid, citrulline and ornithine. The concentration of arginine was critical; at 8 μmol/l the cells remained viable and reversible inhibition could be shown; below this level cells died. L5178Y cells were grown in medium containing from 0 to 80 μmol/l arginine for 24 hours then transferred to fresh medium for 24 hours. Viable cell counts and mitotic indices were determined, and cells were pulsed with (3)H-thymidine, (3)H-uridine, (14)C-leucine and (14)C-arginine at various times. Thymidine uptake was affected most and preceded parallel changes in viable cell numbers. It was concluded that arginine is required by these cells even in a "resting" state and despite some evidence for their capacity to utilize precursors, the tumour cells underwent rapid and extensive destruction when available arginine was severely depleted.
D000818:Animals; D001119:Arginase / Q000633:toxicity; D001120:Arginine / Q000378:metabolism; D002250:Carbon Radioisotopes; D002452:Cell Count; D002460:Cell Line; D002470:Cell Survival; D002478:Cells, Cultured; D002956:Citrulline; D007930:Leucine / Q000378:metabolism; D007939:Leukemia L1210 / Q000378:metabolism; D007945:Leukemia, Lymphoid / Q000378:metabolism; D008228:Lymphoma, Non-Hodgkin; D051379:Mice; D009374:Neoplasms, Experimental; D009952:Ornithine; D013386:Succinates; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D002250:Carbon Radioisotopes; D013386:Succinates; D014316:Tritium; D002956:Citrulline; D001120:Arginine; D009952:Ornithine; D001119:Arginase; D007930:Leucine; D013936:Thymidine; D014529:Uridine