Genetic control of the immune response: in vitro stimulation of lymphocytes by (T,G)-A--L, (H,G)-A--L, and (Phe,G)-A--L.
In vitro antigen-induced tritiated thymidine uptake has been used to study the response of sensitized lymphocytes to (T,G)-A--L, (H,G)-A--L, and (Phe,G)-A--L in responder and nonresponder strains of mice. The reaction is T-cell and macrophage dependent. Highly purified T cells (91% Thy 1.2 positive) are also responsive, suggesting that this in vitro lymphocyte transformation system is not B-cell dependent. Lymphocytes from high and low responder mice stimulated in vitro react as responders and nonresponders in a pattern identical to that seen with in vivo immunization. Stimulation occurs only if soluble antigen is added at physiological temperatures; antigen exposure at 4 degrees C followed by washing and incubation at 37 degrees C fails to induce lymphocyte transformation. Stimulation is specific for the immunizing antigen and does not exhibit the serologic cross-reactivity which is characteristic of these three antigens and their respective antisera. The reaction can be inhibited by anti-H-2 sera but not by anti-immunoglobulin sera. The anti-immunoglobulin sera did, however, inhibit lipopolysaccharide or pokeweed mitogen stimulation. These results suggest that the Ir-1A gene(s) are expressed in T cells, and that there are fundamental physiologic differences between T- and B-cell antigen recognition.
D000818:Animals; D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000008:administration & dosage; D001666:Binding Sites, Antibody; D003429:Cross Reactions; D008040:Genetic Linkage; D005838:Genotype; D006649:Histocompatibility Antigens; D007114:Immunization; D007700:Kinetics; D008213:Lymphocyte Activation; D051379:Mice; D008815:Mice, Inbred Strains; D010455:Peptides / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D013696:Temperature; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D000941:Antigens; D006649:Histocompatibility Antigens; D010455:Peptides; D014316:Tritium; D013936:Thymidine