title: Characterization of the antibodies detected by the microscopic agglutination test for bovine leptospirosis.
abstract: The nature of the antibodies detectable by the microscopic agglutination test for bovine leptospirosis was examined. Density gradient ultracentrifugation, gel filtration and disulphide-bond-reduction experiments indicated that antileptospiral agglutinating activity was present in both IgM and IgG immunoglobulin fractions. This was confirmed by selective precipitation of specific antibody classes and ion-exchange chromatography.
mesh_terms: D000372:Agglutination Tests / Q000379:methods; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000302:isolation & purification; D007922:Leptospirosis / Q000276:immunology* / Q000662:veterinary*; D011817:Rabbits / Q000276:immunology; D014895:Weil Disease / Q000276:immunology / Q000662:veterinary
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M