Role of the polyadenylate segment in the translation of globin messenger RNA in Xenopus oocytes.
The translations of native messenger RNA for rabbit globin and that of poly(A)-free globin messenger RNA have been compared after injection into Xenopus oocytes. The initial rate of translation of poly(A)-free mRNA is close to that found with intact mRNA. However, at longer incubation periods, the rate of globin synthesis with poly(A)-free mRNA is considerably lower than with native mRNA. Similar differences in the template activity of the two mRNA preparations were found with a cell-free extract of Krebs II ascites tumor. It is concluded that the presence of the 3' poly(A)-rich sequence in mRNA is required to ensure high functional stability.
D000227:Adenine Nucleotides / Q000378:metabolism*; D000818:Animals; D001001:Anura; D002287:Carcinoma, Krebs 2 / Q000378:metabolism; D002474:Cell-Free System; D002850:Chromatography, Gel; D005260:Female; D005914:Globins / Q000096:biosynthesis*; D006207:Half-Life; D006454:Hemoglobins / Q000096:biosynthesis; D006639:Histidine / Q000378:metabolism; D010063:Ovum / Q000378:metabolism; D011119:Polynucleotides / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000378:metabolism; D011817:Rabbits; D013698:Templates, Genetic; D013997:Time Factors; D014316:Tritium; D014981:Xenopus
D000227:Adenine Nucleotides; D006454:Hemoglobins; D011119:Polynucleotides; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D014316:Tritium; D006639:Histidine; D005914:Globins