New deoxyribonuclease activity after bacteriophage P22 infection.
Extracts from P22-infected and uninfected cultures of Salmonella typhimurium were subjected to deoxyribonucleic acid (DNA)-cellulose and diethylaminoethyl-cellulose chromatography. Comparison of the elution patterns revealed that in infected cells there is a decrease in the amount of nuclease activity specific for denatured DNA and an increase in the amount of nuclease activity specific for native DNA. The latter activity was shown to differ from a similar host enzyme in Mg(2+), Mn(2+), and pH optima. This new activity is not found after infection of a lysogen with a nonvirulent phage or after infection under nonpermissive conditions with P22ts25.1 (a mutant in gene 25 that carries out no known functions other than adsorption and injection) and thus appears to be specified by the phage genome.
D000645:Ammonium Sulfate; D002458:Cell Fractionation; D002848:Chromatography, DEAE-Cellulose; D004254:DNA Nucleotidyltransferases / Q000302:isolation & purification; D003851:Deoxyribonucleases / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D008242:Lysogeny; D008274:Magnesium; D008345:Manganese; D008722:Methods; D009691:Nucleic Acid Denaturation; D010759:Phosphorus Isotopes; D012483:Salmonella Phages / Q000201:enzymology*; D012486:Salmonella typhimurium / Q000201:enzymology*
D010759:Phosphorus Isotopes; D008345:Manganese; D004254:DNA Nucleotidyltransferases; D003851:Deoxyribonucleases; D008274:Magnesium; D000645:Ammonium Sulfate