Studies on Escherichia coli enzymes involved in the synthesis of uridine diphosphate-N-acetyl-muramyl-pentapeptide.
The specific activities of l-alanine:d-alanine racemase, d-alanine:d-alanine ligase, and the l-alanine, d-glutamic acid, meso-diaminopimelic acid, and d-alanyl-d-alanine adding enzymes were followed during growth of Escherichia coli. The specific activities were nearly independent of the growth phase. d-Alanine:d-alanine ligase was inhibited by d-alanyl-d-alanine, d-cycloserine, glycine, and glycyl-glycine. l-Alanine:d-alanine racemase was found to be sensitive to d-cycloserine, glycine, and glycyl-glycine. The l-alanine adding enzyme was inhibited by glycine and glycyl-glycine.
D000085:Acetates; D000409:Alanine; D000596:Amino Acids; D002247:Carbon Isotopes; D002854:Chromatography, Paper; D003523:Cycloserine; D004151:Dipeptides; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development; D005827:Genetics, Microbial; D005944:Glucosamine; D005971:Glutamates; D005998:Glycine; D007535:Isomerases / Q000037:antagonists & inhibitors; D007700:Kinetics; D008025:Ligases / Q000037:antagonists & inhibitors; D009154:Mutation; D009702:Nucleoside Diphosphate Sugars; D010457:Peptidoglycan / Q000096:biosynthesis; D010867:Pimelic Acids; D013696:Temperature; D014500:Uracil Nucleotides
D000085:Acetates; D000596:Amino Acids; D002247:Carbon Isotopes; D004151:Dipeptides; D005971:Glutamates; D009702:Nucleoside Diphosphate Sugars; D010457:Peptidoglycan; D010867:Pimelic Acids; D014500:Uracil Nucleotides; D003523:Cycloserine; D007535:Isomerases; D008025:Ligases; D005944:Glucosamine; D000409:Alanine; D005998:Glycine