Active suppression of immunoglobulin allotype synthesis. 3. Identification of T cells as responsible for suppression by cells from spleen, thymus, lymph node, and bone marrow.
Thymus-derived cells (T cells) that actively suppress production of IgG2a immunoglobulins carrying the Ig-1b allotype have been found in adult (SJL x BALB/c)F(1) mice exposed to anti-Ig-1b early in life. The suppression is specific for Ig-1b. The allelic product, Ig-1a, is unaffected. Spleen, lymph node, bone marrow, or thymus cells from suppressed mice suppress production of Ig-1b by syngeneic spleen cells from normal F(1) mice. When a mixture of suppressed and normal cells is transferred into lethally irradiated BALB/c mice, there is a short burst of Ig-1b production after which Ig-1b levels in the recipient fall rapidly below detectability. Pretreatment of the cells from the suppressed mice with antiserum specific for T cells (anti-Thy-1b) plus complement before mixture destroys the suppressing activity. Similar results with suppressor cells were obtained in vitro using Mishell-Dutton cultures. Mixture of spleen cells from suppressed animals with sheep erythrocyte (SRBC)-primed syngeneic normal spleen before culture suppresses Ig-1b plaque-forming cell (PFC) formation while leaving Ig-1a PFC unaffected. Treatment of the suppressed spleen with anti-Thy-1b before transfer removes the suppressing activity.
D000818:Animals; D001853:Bone Marrow / Q000276:immunology*; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D006168:Guinea Pigs; D007106:Immune Sera; D007070:Immunoglobulin A / Q000032:analysis; D007136:Immunoglobulins / Q000096:biosynthesis*; D007165:Immunosuppression Therapy; D008198:Lymph Nodes / Q000276:immunology*; D051379:Mice; D011863:Radioimmunoassay; D013154:Spleen / Q000276:immunology* / Q000528:radiation effects / Q000637:transplantation; D013601:T-Lymphocytes / Q000276:immunology*
D007106:Immune Sera; D007070:Immunoglobulin A; D007136:Immunoglobulins