Further studies on the anti-immunoglobulin G hemagglutination-inhibition test for influenza.
Incorporating species-specific anti-immunoglobulin G (IgG) serum into the hemagglutination-inhibition (HI) test for influenza markedly increased sensitivity without loss of specificity. The effectiveness of anti-IgG serum for augmenting antibody titers may be influenced by the variable potency of commercial anti-IgG preparations. Maximal enhancement of HI titers was achieved when anti-IgG serum and virus-antiserum mixtures were incubated at 23 C for 10 min. Precision and reproducibility of the test were within acceptable limits. Other conditions likely to affect the test were investigated.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D002021:Buffers; D002642:Chick Embryo; D002645:Chickens / Q000276:immunology; D003429:Cross Reactions; D004912:Erythrocytes / Q000276:immunology; D006041:Goats / Q000276:immunology; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007251:Influenza, Human / Q000175:diagnosis / Q000276:immunology*; D008722:Methods; D009975:Orthomyxoviridae / Q000254:growth & development / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D012698:Serologic Tests; D013997:Time Factors
D000888:Antibodies, Anti-Idiotypic; D002021:Buffers; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M