title: Cell division of Escherichia coli BUG-6: effect of varying the temperature used as the nonpermissive growth condition.
abstract: When Escherichia coli BUG-6 is shifted from 30 C to 36 or 38 C, division does not stop, but the rate of division of the cell population is initially decreased followed by a period of increased rate of division before the rates characteristic of growth at 36 and 38 C are obtained. After a shift from 30 to 40 C, the rate of cell division gradually decreases over a 10-min period and then stops. The inhibition continues for 25 min, and then the cells divide rapidly before the division rate characteristic of 40 C is obtained. If filaments produced by 45 min of growth at 42 C are temporarily replaced at 30 C and then returned to 42 C, division occurs at 42 C. The amount of division is dependent on the length of the period at 30 C and can be decreased by a 3-min pulse of chloramphenicol immediately before the 42 to 30 C shift.
mesh_terms: D002455:Cell Division / Q000187:drug effects; D002701:Chloramphenicol / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development*; D007700:Kinetics; D008954:Models, Biological; D013696:Temperature; D013997:Time Factors
chemical_list: D002701:Chloramphenicol