title: Biosynthesis of riboflavine in Saccharomyces cerevisiae: the role of genes rib 1  and rib 7 .
abstract: Haploid strains of Saccharomyces cerevisiae with mutations in two different rib genes were constructed. These strains were studied by tetrad analysis and by quantitative determination of accumulation products. The genes rib(1), rib(7), and rib(2) are not linked to each other. rib(1)-rib(7) strains and rib(1)-rib(2) strains exhibit the phenotypic properties of rib(1) strains. rib(7)-rib(2) strains show the phenotypic properties of rib(7) strains. The results support the conclusion that the genes rib(1) and rib(7) code for the first and second enzyme of the riboflavine pathway, respectively.
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D002855:Chromatography, Thin Layer; D003470:Culture Media; D005285:Fermentation; D005796:Genes; D005815:Genetic Code; D005816:Genetic Complementation Test; D005827:Genetics, Microbial; D006238:Haploidy; D007202:Indicators and Reagents; D009153:Mutagens; D009154:Mutation; D010641:Phenotype; D011621:Pteridines / Q000032:analysis / Q000302:isolation & purification; D011743:Pyrimidines / Q000096:biosynthesis; D012256:Riboflavin / Q000096:biosynthesis*; D012440:Saccharomyces / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism
chemical_list: D003470:Culture Media; D007202:Indicators and Reagents; D009153:Mutagens; D011621:Pteridines; D011743:Pyrimidines; D012256:Riboflavin