title: Dependence of ribonucleic acid synthesis on continuous protein synthesis in yeast.
abstract: Using an auxotrophic strain of Saccharomyces cerevisiae, we examined the kinetics of ribonucleic acid (RNA) synthesis following inhibition of protein synthesis caused by amino acid starvation or cycloheximide. Removal of a required amino acid immediately stopped net protein synthesis. After a brief lag, RNA synthesis also ceased. Cycloheximide, a ribosome-inhibiting drug, also immediately halted net protein synthesis. Again RNA synthesis stopped after a brief lag. Although cycloheximide and amino acid starvation affect different steps in protein biosynthesis, both inhibited RNA synthesis in identical fashion. This indicates that amino acids do not play a unique role in the control of RNA production in rapidly growing yeast; rather, it suggests that RNA synthesis is responsive to the overall rate of protein synthesis itself.
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D002247:Carbon Isotopes; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology; D008239:Lysine / Q000378:metabolism; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D012440:Saccharomyces / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D013053:Spectrophotometry; D014316:Tritium; D014443:Tyrosine / Q000378:metabolism; D014498:Uracil / Q000378:metabolism
chemical_list: D000596:Amino Acids; D002247:Carbon Isotopes; D003470:Culture Media; D014316:Tritium; D014443:Tyrosine; D014498:Uracil; D012313:RNA; D003513:Cycloheximide; D008239:Lysine