Effect of phenethyl alcohol on deoxyribonucleic acid-membrane association in Escherichia coli.
The effect of phenethyl alcohol (PEA) on deoxyribonucleic acid (DNA)-membrane attachment was examined by means of the "M-band" technique of Tremblay, Daniels, and Schaechter. The results of this work indicate that no significant release of DNA from the membrane could be detected after treatment with 0.25% PEA, a concentration previously shown to prevent reinitiation of DNA replication. However, concentrations of 0.5% PEA and greater, which stop DNA replication immediately, appear to release 25 to 80% of the DNA from the membrane.
D000438:Alcohols / Q000494:pharmacology*; D001555:Benzene Derivatives / Q000494:pharmacology; D002247:Carbon Isotopes; D002462:Cell Membrane / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D003470:Culture Media; D004261:DNA Replication / Q000187:drug effects; D004269:DNA, Bacterial / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D005374:Filtration; D013395:Sucrose; D013941:Thymine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium
D000438:Alcohols; D001555:Benzene Derivatives; D002247:Carbon Isotopes; D003470:Culture Media; D004269:DNA, Bacterial; D014316:Tritium; D013395:Sucrose; D013941:Thymine