title: Utilization of  -aminobutyric acid as the sole carbon and nitrogen source by Escherichia coli K-12 mutants.
abstract: Wild-type strains of Escherichia coli K-12 cannot grow in media with gamma-aminobutyrate (GABA) as the sole source of carbon or nitrogen. Mutants were isolated which could utilize GABA as the sole source of nitrogen. These mutants were found to have six- to ninefold higher activities of gamma-aminobutyrate-alpha-ketoglutarate transaminase (EC 2.6.1.19) and succinate semialdehyde dehydrogenase (EC 1.2.1.16) than those of the wild-type parent strains. Secondary mutants derived from these GABA-nitrogen-utilizing strains were able to grow on GABA as the sole source of carbon and nitrogen. They also grew faster on a variety of other carbon and nitrogen sources, and their growth was more strongly inhibited by different metabolic inhibitors than was that of the parent strains. The nature of the two mutations and the possible genes involved are discussed. A scheme of the pathway for GABA breakdown in E. coli K-12 is presented.
mesh_terms: D000085:Acetates / Q000494:pharmacology; D000613:Aminobutyrates / Q000378:metabolism*; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001224:Aspartic Acid; D002244:Carbon / Q000378:metabolism*; D002247:Carbon Isotopes; D002474:Cell-Free System; D002701:Chloramphenicol / Q000494:pharmacology; D003470:Culture Media; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D005827:Genetics, Microbial; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D005971:Glutamates / Q000494:pharmacology; D008190:Lyases / Q000378:metabolism; D008956:Models, Chemical; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D011691:Puromycin / Q000494:pharmacology; D000637:Transaminases / Q000378:metabolism
chemical_list: D000085:Acetates; D000613:Aminobutyrates; D002247:Carbon Isotopes; D003470:Culture Media; D005971:Glutamates; D001224:Aspartic Acid; D011691:Puromycin; D002701:Chloramphenicol; D002244:Carbon; D010088:Oxidoreductases; D005969:Glutamate Dehydrogenase; D000637:Transaminases; D001219:Aspartate Aminotransferases; D008190:Lyases; D009584:Nitrogen; D004121:Dimethyl Sulfoxide