Enhancement of DNA synthesis in a mammalian cell-free system by trypsin treatment.
DNA synthesis in a broken cellular preparation of Chinese hamster cells was enhanced approximately 10-fold by a brief trypsin treatment. Alphachymotrypsin also enhanced the synthesis, whereas Pronase did not. The trypsin appears to be acting on a component of the nucleus. Evidence suggests that the trypsin is not removing protein from the DNA, but may be activating the system some other way.
D000818:Animals; D002460:Cell Line; D002474:Cell-Free System; D002918:Chymotrypsin / Q000494:pharmacology; D006224:Cricetinae; D004247:DNA / Q000096:biosynthesis*; D005347:Fibroblasts; D007700:Kinetics; D011402:Pronase / Q000494:pharmacology; D013268:Stimulation, Chemical; D014357:Trypsin / Q000494:pharmacology*
D004247:DNA; D002918:Chymotrypsin; D014357:Trypsin; D011402:Pronase