Purification and partial characterization of a bacteriocin from Serratia marcescens.
Bacteriocin JF246 has been purified from mitomycin C-induced Serratia marcescens cells by salt extraction, ammonium sulfate fractionation, and chromatography on QAE-Sephadex and SP-Sephadex. The purified material is homogeneous on polyacrylamide gel electrophoresis in the presence of 2% sodium dodecyl sulfate or 6 m urea. In the absence of these agents, the bacteriocin associates into aggregates which can be dissociated with 0.4 m NaCl. The bacteriocin is probably composed of a single subunit with a molecular weight of 64,000 daltons. Analytical studies show the bacteriocin to be essentially protein in nature containing less than one residue of glucose or phosphorus per 64,000 daltons.
D000596:Amino Acids / Q000032:analysis; D000645:Ammonium Sulfate; D001430:Bacteriocins / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification* / Q000494:pharmacology; D002849:Chromatography, Gas; D002852:Chromatography, Ion Exchange; D004589:Electrophoresis, Disc; D005947:Glucose / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008937:Mitomycins / Q000494:pharmacology; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000096:biosynthesis; D010758:Phosphorus / Q000032:analysis; D012706:Serratia marcescens / Q000032:analysis* / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D012965:Sodium Chloride; D012967:Sodium Dodecyl Sulfate; D014508:Urea
D000596:Amino Acids; D001430:Bacteriocins; D008937:Mitomycins; D010758:Phosphorus; D012967:Sodium Dodecyl Sulfate; D012965:Sodium Chloride; D014508:Urea; D010447:Peptide Hydrolases; D005947:Glucose; D000645:Ammonium Sulfate