Twenty-four-hour immunofluorescence technique for the detection of salmonellae in nonfat dry milk.
A detection procedure was developed in which a newly devised lysine-iron medium was used as a one-step selective and enrichment medium for detection of salmonellae by the fluorescent-antibody technique. Incubation was conducted in two steps: initially at 30 C for 5 hr to resuscitate sublethally stressed cells, followed by incubation at 39 C for 17 hr. Twenty-seven strains of salmonellae from groups A-I were utilized in the development of this procedure which was sensitive enough to detect one Salmonella bacterium in 100 g of nonfat dry milk.
D000818:Animals; D004396:Coloring Agents; D003470:Culture Media; D004352:Drug Resistance, Microbial; D004755:Enterobacteriaceae / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D005516:Food Microbiology; D007106:Immune Sera; D007501:Iron; D008239:Lysine; D008722:Methods; D008892:Milk; D009675:Novobiocin / Q000494:pharmacology; D012475:Salmonella / Q000187:drug effects / Q000276:immunology / Q000302:isolation & purification*; D012703:Serotyping; D013045:Species Specificity; D013997:Time Factors
D004396:Coloring Agents; D003470:Culture Media; D007106:Immune Sera; D009675:Novobiocin; D007501:Iron; D008239:Lysine