Hormonal control of messenger ribonucleic acid metabolism in barley aleurone layers.
Ribonucleic acid containing poly(adenylic acid) [poly(A)-RNA] is present in barley aleurone layers. This poly(A)-RNA becomes labeled with radioactive precursors of RNA during the incubation of isolated aleurone layers with or without gibberellic acid. However, the rate of synthesis of poly(A)-RNA is enhanced by gibberellic acid. This enhancement begins within 3-4 hr of addition of the hormone and reaches a maximum, which is about 50-60% over the control, 10-12 hr after addition of the hormone. Cordycepin inhibits total RNA as well as poly(A)-RNA synthesis in barley aleurone layers. However, cordycepin inhibits the hormone-controlled synthesis of alpha-amylase (EC 3.2.1.1) only if it is added 12 hr or less after gibberellic acid. The insensitivity of alpha-amylase production to cordycepin after 12 hr of gibberellic acid treatment suggests that alpha-amylase is translated from stable messenger RNA.
D000227:Adenine Nucleotides / Q000032:analysis; D000241:Adenosine / Q000378:metabolism; D000681:Amylases / Q000096:biosynthesis; D003839:Deoxyadenosines / Q000494:pharmacology; D003864:Depression, Chemical; D005875:Gibberellins / Q000494:pharmacology*; D001467:Hordeum; D010940:Plant Proteins / Q000096:biosynthesis; D010944:Plants / Q000378:metabolism*; D011119:Polynucleotides / Q000032:analysis; D012333:RNA, Messenger / Q000096:biosynthesis*; D014316:Tritium
D000227:Adenine Nucleotides; D003839:Deoxyadenosines; D005875:Gibberellins; D010940:Plant Proteins; D011119:Polynucleotides; D012333:RNA, Messenger; D014316:Tritium; D000681:Amylases; D000241:Adenosine