title: Mechanism of gonadotropin action in amphibia: involvement of mitochondria.
abstract: Luteinizing hormone (a pituitary gonadotropic hormone) stimulates Delta(5)-3beta- hydroxysteroid dehydrogenase activity in the microsomal fraction of frog testes when incubated together with the mitochondria; incubation together with the nuclei instead of the mitochondria does not result in increased, Delta(5)-3beta-hydroxysteroid dehydrogenase activity. The increase is not, induced by adenosine triphosphate, it appears to be hormone-specific, and it is sensitive to puromycin and actinomycin D. These data suggest that the mitochondrial DNA may be involved in mediating the action of luteinizing hormone in amphibian steroidogenesis.
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001001:Anura; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000201:enzymology; D002498:Centrifugation; D003609:Dactinomycin / Q000494:pharmacology; D006913:Hydroxysteroid Dehydrogenases / Q000032:analysis*; D066298:In Vitro Techniques; D007986:Luteinizing Hormone / Q000494:pharmacology*; D008297:Male; D008861:Microsomes / Q000201:enzymology*; D008928:Mitochondria / Q000201:enzymology*; D011691:Puromycin / Q000494:pharmacology; D011894:Rana pipiens; D013737:Testis / Q000166:cytology / Q000201:enzymology*; D013972:Thyrotropin / Q000494:pharmacology
chemical_list: D003609:Dactinomycin; D011691:Puromycin; D000255:Adenosine Triphosphate; D007986:Luteinizing Hormone; D013972:Thyrotropin; D006913:Hydroxysteroid Dehydrogenases