title: Fixation of mature spores of Clostridium botulinum.
abstract: A triple fixation method using a sequential application of 15 or 30% formaldehyde, 6% glutaraldehyde, and 1% osmium tetroxide resulted in excellent fixation of mature spores of Clostridium botulinum.
mesh_terms: D000447:Aldehydes; D001431:Bacteriological Techniques; D002473:Cell Wall; D003014:Clostridium botulinum / Q000166:cytology*; D005557:Formaldehyde; D008854:Microscopy, Electron; D009992:Osmium; D010087:Oxides; D013170:Spores / Q000166:cytology*; D013171:Spores, Bacterial / Q000166:cytology
chemical_list: D000447:Aldehydes; D010087:Oxides; D005557:Formaldehyde; D009992:Osmium