Stability of neuronal microtubules to high pressure in vivo and in vitro.
Neuronal microtubules in a variety of nerve cell types are unaffected by high hydrostatic pressures over a range of 1400-10,000 pounds/inch(2) and periods of 10-45 min. Similarly, purified tubulin polymerized to form microtubules in vitro were not depolymerized by the same range of pressures. The depolymerization of microtubules in several types of non-neuronal cells, which has been reported, may have been over-generalized with regard to the direct action of pressure on microtubule stability.
D000818:Animals; D001369:Axons / Q000648:ultrastructure; D002531:Cerebellum / Q000648:ultrastructure; D003712:Dendrites / Q000648:ultrastructure; D006874:Hydrostatic Pressure; D008854:Microscopy, Electron; D008870:Microtubules / Q000502:physiology*; D009419:Nerve Tissue Proteins; D009474:Neurons / Q000648:ultrastructure*; D009832:Olfactory Nerve / Q000648:ultrastructure; D011108:Polymers; D011312:Pressure; D011689:Purkinje Cells / Q000648:ultrastructure; D011894:Rana pipiens; D013153:Splanchnic Nerves / Q000648:ultrastructure
D009419:Nerve Tissue Proteins; D011108:Polymers