Chromosomal localization of the structural genes of the polypeptide chain elongation factors.
A survey of the polypeptide chain elongation factors in potentially sexually compatible genera was carried out. Factors from Escherichia coli and Proteus mirabilis were found to be clearly distinguishable by immunochemical and electrophoretic techniques. Mapping of the structural genes of these factors was undertaken by a study of the gene products in genetically defined E. coli-P. mirabilis hybrid diploid strains. It was found that the EF G factor mapped within 5 min of the streptomycin resistance locus, but the EF Ts factor did not map in this region.
D001426:Bacterial Proteins / Q000302:isolation & purification; D002874:Chromosome Mapping; D004171:Diploidy; D004589:Electrophoresis, Disc; D004926:Escherichia coli; D005796:Genes; D005827:Genetics, Microbial; D006238:Haploidy; D006824:Hybridization, Genetic; D007700:Kinetics; D009154:Mutation; D010452:Peptide Biosynthesis; D010441:Peptide Chain Elongation, Translational; D011233:Precipitin Tests; D011511:Proteus; D011513:Proteus mirabilis; D013045:Species Specificity
D001426:Bacterial Proteins