Differentiation between intracellular and cell surface glycosyl transferases: galactosyl transferase activity in intact cells and in cell homogenate.
Intact BHK (baby hamster kidney) cells catalyze the hydrolysis of UDP-galactose to free galactose. The generation of galactose from UDP-galactose and its intracellular utilization impede the detection of possible galactosyl transferases on the cell surface of intact cells. Several independent procedures have been used to distinguish between intracellular and cell surface glycosyl transferases. With these procedures, no evidence was obtained for the presence of detectable amounts of galactosyl transferase activity on the surface of BHK cells. The data suggest that galactosyl transferases do not play a general role in the phenomena of cell adhesion and contact inhibition.
D000818:Animals; D002250:Carbon Radioisotopes; D002448:Cell Adhesion; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology*; D002854:Chromatography, Paper; D003260:Contact Inhibition; D006224:Cricetinae; D005690:Galactose; D005964:Glucosyltransferases / Q000378:metabolism; D006602:Hexosyltransferases / Q000378:metabolism*; D006868:Hydrolysis; D007668:Kidney; D008274:Magnesium / Q000378:metabolism; D014316:Tritium; D014539:Uridine Diphosphate Sugars / Q000378:metabolism
D002250:Carbon Radioisotopes; D014539:Uridine Diphosphate Sugars; D014316:Tritium; D005964:Glucosyltransferases; D006602:Hexosyltransferases; D008274:Magnesium; D005690:Galactose