title: Antigenic properties of murine sarcoma virus-transformed BALB-3T3 nonproducer cells.
abstract: The isolation of clonal lines of murine sarcoma virus-transformed, non-producer BALB/3T3 cells has provided a model system for determining whether RNA tumor virus-transformed cells possess virus-specific transplantation antigens. MSV nonproducer cells (K-234) were clonally derived from an inbred mouse cell line, BALB/3T3. A parallel virus-producing cell line was obtained by infection of the MSV nonproducer cells with Rauscher leukemia virus. K-234 was much more tumorigenic than K-234(R). Preimmunization of syngeneic mice with either K-234(R) or with UV-inactivated Rauscher leukemia virus induced transplantation resistance to subsequent challenge with K-234(R), but not with K-234. In contrast, mice preimmunized with nonproducer cells were not made resistant to subsequent challenge with the homologous cells. Antisera prepared from mice immunized with K-234(R) were specifically cytotoxic and positive by fluorescent antibody staining for K-234(R) target cells, but not to either BALB/3T3 or K-234. The results show that MSV nonproducer cells lack detectable transplantation antigens and suggest that the transplantation resistance to the producing cells is attributable to maturing virus at the cell surface.
mesh_terms: D000818:Animals; D000941:Antigens; D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D002460:Cell Line / Q000276:immunology*; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D005455:Fluorescent Antibody Technique; D051379:Mice; D008815:Mice, Inbred Strains; D008979:Moloney murine leukemia virus / Q000276:immunology* / Q000472:pathogenicity; D009500:Neutralization Tests; D012513:Sarcoma, Experimental / Q000276:immunology*; D014181:Transplantation Immunology
chemical_list: D000941:Antigens; D000951:Antigens, Neoplasm; D000956:Antigens, Viral