Induction of the ara operon of Escherichia coli B-r.
The inducer specificity and kinetics of induction of the ara operon were examined in Escherichia coli B/r. A difference in the kinetics of induction was found between our B/r strains and the K-12 strain previously described by Schleif. The roles of active transport and metabolism of inducer, and of cell density, in induction were studied. d-Fucose and beta-methyl-l-arabinoside were competitive inhibitors of induction. No inducer of the wild-type strain other than l-arabinose was found. However, a procedure for selecting mutants with altered inducer affinity or specificity was developed. The properties of one such mutant (inducible by d-fucose) are described.
D001089:Arabinose / Q000378:metabolism* / Q000494:pharmacology; D001693:Biological Transport, Active; D002247:Carbon Isotopes; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D004790:Enzyme Induction; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D005643:Fucose / Q000494:pharmacology; D005827:Genetics, Microbial; D005838:Genotype; D006027:Glycosides / Q000494:pharmacology; D007535:Isomerases / Q000096:biosynthesis*; D007700:Kinetics; D026901:Membrane Transport Proteins; D009154:Mutation; D009876:Operon; D010429:Pentoses; D010641:Phenotype; D010770:Phosphotransferases / Q000096:biosynthesis*; D013045:Species Specificity
D002247:Carbon Isotopes; D006027:Glycosides; D026901:Membrane Transport Proteins; D010429:Pentoses; D005643:Fucose; D001089:Arabinose; D010770:Phosphotransferases; D007535:Isomerases