Ligand-induced movement of lymphocyte surface macromolecules. IV. Stimulation of cell motility by anti-Ig and lack of relationship to capping.
Microscopic examination of spleen lymphocytes discloses a small number moving at random at a given time. The majority of lymphocytes with this spontaneous movement are thymic derived. Addition of anti-Ig antibodies stimulates random movement of B lymphocytes. This movement depends upon a bivalent antibody and a metabolically active cell. The movement is inhibited by DFP, suggesting the involvement of a serine esterase. Also the anti-Ig stimulated movement of the lymphocyte is inhibited by cytochalasin B or by not allowing the cells to settle onto a surface. Lymphocytes treated with DFP or cytochalasin B, or untreated lymphocytes in suspension, capped the anti-Ig-Ig complexes. Hence, one can dissociate the surface capping of anti-Ig-Ig complexes from cell movement. We postulate that capping may result from superficial movements of the surface and/or from membrane flow, both of which are not related to actual translation of the cell on a surface. Four effects have now been observed following combination of a ligand with the antigen receptor on the B lymphocytes: redistribution on the surface of the complexes; pinocytosis and catabolism; shedding into the extracellular environment; and stimulation of translational movement.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000936:Antigen-Antibody Complex; D001402:B-Lymphocytes / Q000276:immunology; D001666:Binding Sites, Antibody; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007461:Iodoacetates / Q000494:pharmacology; D007531:Isoflurophate / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D009840:Oligomycins / Q000494:pharmacology; D011817:Rabbits / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D013696:Temperature
D000888:Antibodies, Anti-Idiotypic; D000936:Antigen-Antibody Complex; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007461:Iodoacetates; D009840:Oligomycins; D007531:Isoflurophate; D003571:Cytochalasin B; D005947:Glucose; D003078:Colchicine