title: Restoration of antibody-forming capacity in cultures of nonadherent spleen cells by mercaptoethanol.
abstract: Populations of mouse spleen cells, without those cells that adhere to glass or plastic, exhibit little or no capacity for formation of antibody to sheep erythrocytes in the Mishell-Dutton culture system. When 5 x 10(-5)M mercaptoethanol is added to the culture medium, the antibody-forming capacity of these nonadherent spleen cells is restored to that of unfractionated spleen cells.
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D000921:Antibody-Producing Cells / Q000187:drug effects*; D002478:Cells, Cultured; D006462:Hemolytic Plaque Technique; D008623:Mercaptoethanol / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000166:cytology / Q000276:immunology*
chemical_list: D008623:Mercaptoethanol