Aerobic reduction of cytochrome b 566 in pigeon-heart mitochondria (succinate-cytochrome C1 reductase-stopped-flow kinetics).
In anaerobic, uncoupled pigeon-heart mitochondria treated with oxidizable substrate, the cytochrome b(566) remains largely oxidized. In the presence of antimycin A, addition of oxygen induces a reduction of this cytochrome. The rate of cytochrome b(566) reduction is comparable to and dependent on the rate of cytochrome c(1) oxidation. Kinetic data suggest that either ubiquinone or another donor of similar potential provides electrons for the reduction of cytochrome b(566). It is postulated that the aerobic reduction of cytochrome b(566) is directly related to the energy conservation at site II.
D000332:Aerobiosis; D000693:Anaerobiosis; D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D002499:Centrifugation, Density Gradient; D010856:Columbidae; D003580:Cytochromes / Q000378:metabolism*; D007700:Kinetics; D008931:Mitochondria, Muscle / Q000187:drug effects / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000494:pharmacology; D010544:Peroxidases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013057:Spectrum Analysis; D014451:Ubiquinone / Q000494:pharmacology; D014466:Ultraviolet Rays
D003580:Cytochromes; D014451:Ubiquinone; D000968:Antimycin A; D010544:Peroxidases; D010100:Oxygen