Postreplication repair of ultraviolet damage in Haemophilus influenzae.
The deoxyribonucleic acid (DNA) synthesized following ultraviolet (UV) irradiation of wild-type (Rd) and recombination-defective strains of Haemophilus influenzae has been analyzed by alkaline sucrose gradient sedimentation. Strain Rd and a UV-resistant, recombination-defective strain Rd(DB117) (rec-) are able to carry out postreplication repair, i.e., close the single-strand gaps in the newly synthesized DNA; in the UV-sensitive, recombination-defective strain DB117, the gaps remain open. The lack of postreplication repair in this strain may be the result of degradation of the newly synthesized DNA.
D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D004260:DNA Repair; D004261:DNA Replication; D004269:DNA, Bacterial / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D004277:DNA, Single-Stranded / Q000032:analysis; D005827:Genetics, Microbial; D006193:Haemophilus influenzae / Q000032:analysis / Q000378:metabolism* / Q000528:radiation effects; D009154:Mutation; D009691:Nucleic Acid Denaturation; D011830:Radiation Effects; D011995:Recombination, Genetic; D013395:Sucrose; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014466:Ultraviolet Rays
D002247:Carbon Isotopes; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D014316:Tritium; D013395:Sucrose; D013936:Thymidine