Nuclease activities of Mycoplasma gallisepticum as a function of culture age in different media.
Levels of deoxyribonuclease and ribonuclease activities in the supernatant (soluble plus ribosomes) fraction of Mycoplasma gallisepticum were assayed and found to be a function of strain, nutrient, and culture age. In yeast hydrolysate-enriched broth, maximal nuclease activities occurred during exponential growth.
D000818:Animals; D001431:Bacteriological Techniques; D002462:Cell Membrane / Q000201:enzymology; D002474:Cell-Free System; D003470:Culture Media; D003593:Cytoplasm / Q000201:enzymology; D004269:DNA, Bacterial / Q000032:analysis; D003851:Deoxyribonucleases / Q000378:metabolism*; D005947:Glucose; D006736:Horses; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera; D007773:Lactates; D009174:Mycoplasma / Q000032:analysis / Q000201:enzymology* / Q000254:growth & development; D010406:Penicillins; D010635:Phenolphthaleins; D012329:RNA, Bacterial / Q000032:analysis; D012260:Ribonucleases / Q000378:metabolism*; D012270:Ribosomes / Q000201:enzymology; D012440:Saccharomyces; D013045:Species Specificity; D013997:Time Factors
D003470:Culture Media; D004269:DNA, Bacterial; D007106:Immune Sera; D007773:Lactates; D010406:Penicillins; D010635:Phenolphthaleins; D012329:RNA, Bacterial; D003851:Deoxyribonucleases; D012260:Ribonucleases; D005947:Glucose