title: 8(9),22 -Ergostadiene-3 -ol, an ergosterol precursor accumulated in wild-type and mutants of yeast.
abstract: Whereas wild-type strains of Saccharomyces cerevisiae can synthesize up to 7% dry weight of ergosterol, a polyene-resistant mutant has been obtained which produces no ergosterol. Instead, a C-28 methyl sterol is produced, and it has been identified as Delta(8(9),22)-ergostadiene-3beta-ol. This sterol is converted to ergosterol by wild-type yeasts and is observed transiently in cells during aerobic adaption of anaerobically grown wild-type yeasts. The new sterol is proposed as an intermediate in ergosterol biosynthesis.
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D002247:Carbon Isotopes; D002855:Chromatography, Thin Layer; D004872:Ergocalciferols / Q000096:biosynthesis*; D004875:Ergosterol / Q000096:biosynthesis; D005827:Genetics, Microbial; D008715:Methionine / Q000378:metabolism; D009154:Mutation; D010429:Pentoses; D012440:Saccharomyces / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013053:Spectrophotometry; D013261:Sterols / Q000378:metabolism*; D013402:Sugar Alcohols; D014466:Ultraviolet Rays
chemical_list: D002247:Carbon Isotopes; D004872:Ergocalciferols; D010429:Pentoses; D013261:Sterols; D013402:Sugar Alcohols; D008715:Methionine; D004875:Ergosterol