Direct determination of the lamellar structure of peripheral nerve myelin at moderate resolution (7A).
Low-angle X-ray diffraction patterns have been recorded from normal nerve and nerve swollen in glycerol solutions. The new X-ray data have a resolution of 7 A. Direct methods of structure analysis which include deconvolution of the auto-correlation function and sampling theorem reconstructions have been used in the interpretation of the X-ray data. Phases have been assigned to the first 12 orders of diffraction from normal nerve. Fourier syntheses at a resolution of 7 A are described and an absolute electron density scale is derived. A possible molecular interpretation of the electron density profile is given.
D000818:Animals; D003720:Densitometry; D005583:Fourier Analysis; D005990:Glycerol; D008433:Mathematics; D008954:Models, Biological; D008968:Molecular Conformation; D009186:Myelin Sheath / Q000032:analysis*; D010525:Peripheral Nerves / Q000032:analysis*; D011817:Rabbits; D011894:Rana pipiens; D012584:Sciatic Nerve; D013045:Species Specificity; D014961:X-Ray Diffraction
D005990:Glycerol