Estimation of the half-life of a secretory protein message.
Synthesis of a specific secretory protein was followed in the presence of actinomycin D and cordycepin (3'-deoxyadenosine). The apparent half-life of the messenger RNA for this protein is significantly longer in the presence of actinomycin D than in the presence of cordycepin. These results suggest that actinomycin D studies of half-life in specialized cells may be inaccurate.
D000818:Animals; D003609:Dactinomycin / Q000494:pharmacology*; D003839:Deoxyadenosines / Q000494:pharmacology*; D005998:Glycine / Q000378:metabolism; D007313:Insecta; D007700:Kinetics; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D014316:Tritium
D003839:Deoxyadenosines; D012333:RNA, Messenger; D014316:Tritium; D003609:Dactinomycin; D005998:Glycine