title: Methylated messenger RNA synthesis in vitro by purified reovirus.
abstract: Purified human reovirus contains RNA methylase activity in addition to an RNA polymerase. Virions incubated under appropriate conditions in the presence of S-adenosyl-L-methionine synthesize mRNA that is specifically methylated in the 5'-terminal guanosine. Alkaline digestion of the methylated RNA released a 5'-terminal dinucleotide, ppG'pCp, indicating that the guanosine contains 2'-O-methylribose. The possible roles of methylation in viral and cellular mRNA function is discussed.
mesh_terms: D002848:Chromatography, DEAE-Cellulose; D003470:Culture Media; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D007700:Kinetics; D007739:L Cells / Q000378:metabolism; D008745:Methylation; D009841:Oligonucleotides / Q000302:isolation & purification; D010761:Phosphorus Radioisotopes; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000378:metabolism; D012087:Reoviridae / Q000201:enzymology / Q000378:metabolism*; D012436:S-Adenosylmethionine / Q000378:metabolism; D013268:Stimulation, Chemical; D014316:Tritium; D014633:Valine / Q000378:metabolism; D012359:tRNA Methyltransferases / Q000378:metabolism*
chemical_list: D003470:Culture Media; D009841:Oligonucleotides; D010761:Phosphorus Radioisotopes; D012333:RNA, Messenger; D014316:Tritium; D012436:S-Adenosylmethionine; D012359:tRNA Methyltransferases; D012321:DNA-Directed RNA Polymerases; D014633:Valine