Autoradiographic studies on the proliferation of antibody-producing cells in vitro.
A rapid and reliable autoradiographic technique for plaque-forming cells (PFCs) using (14)C rather than tritiated thymidine is described. Its application to PFCs developing in vitro shows that (a) practically all PFCs derive from precursors dividing steadily during the culture period, (b) PFC precursors divide in the absence of T-cell helper function, and (c) at least some PFCs may continue to divide.
D000818:Animals; D000917:Antibody Formation; D000921:Antibody-Producing Cells / Q000502:physiology*; D001345:Autoradiography; D002250:Carbon Radioisotopes; D002455:Cell Division; D004247:DNA / Q000096:biosynthesis; D007111:Immunity, Cellular; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D002250:Carbon Radioisotopes; D014316:Tritium; D004247:DNA; D013936:Thymidine