title: Positive and negative control of R-factor replication in Proteus mirabilis.
abstract: Replication of the 50 and 58 moles per cent guanine plus cytosine (%GC) components of R factor 222 in Proteus mirabilis during growth in the presence and absence of chloramphenicol and after shifting exponential- and stationary-phase cells to conditions which inhibit host protein or deoxyribonucleic acid (DNA) synthesis was examined. Chloramphenicol reduced the growth rate but increased the amount of both R-factor components; the 58% GC component showed a larger proportionate increase. This was inferred to indicate reduced synthesis of an inhibitor that acts on both R-factor components and an initiator for replication of the 50% GC component. Replicative patterns observed after shifting exponential- and stationary-phase cells grown with or without chloramphenicol to minimal medium or chloramphenicol for one generation, or puromycin for 3 hr, corroborated this interpretation. After shifts of exponential cells from either medium, replication of the 50% GC components paralleled host replication, thus indicating a requirement for protein synthesis; replication of the 58% GC component increased due to reduced inhibitor synthesis. R-factor DNA remained constant after shifting stationary cells from drug-free medium, thus indicating that the cells contained effective concentrations of the regulatory inhibitor, whereas increased replication of the 58% GC component occurred after identical shifts of chloramphenicol-grown cells of the same chronological age. Similar responses were observed after shifts to 5 C or to medium containing streptomycin or tetracycline. Absence of replication of the 50% GC component after shifting to medium containing nalidixic acid or phenethanol and its hereditary persistence during growth indicated that the 50% GC replicon was attached to the membrane. Thus, in P. mirabilis the three replicons of R factor 222 are regulated as follows: The composite and transfer portion (RTF) replicons represented by the 50% GC component require protein synthesis and membrane attachment and are negatively regulated by an inhibitor; the 58% GC or resistance-determinants replicon exists cytoplasmically and is subject only to negative control.
mesh_terms: D000438:Alcohols / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002701:Chloramphenicol / Q000494:pharmacology; D002712:Chlorides; D003470:Culture Media; D003596:Cytosine / Q000032:analysis; D004261:DNA Replication; D004269:DNA, Bacterial / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification; D004352:Drug Resistance, Microbial; D005374:Filtration; D005827:Genetics, Microbial; D006147:Guanine / Q000032:analysis; D008956:Models, Chemical; D008970:Molecular Weight; D009268:Nalidixic Acid / Q000494:pharmacology; D011511:Proteus / Q000187:drug effects*; D011513:Proteus mirabilis / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D011691:Puromycin / Q000494:pharmacology; D013053:Spectrophotometry; D013307:Streptomycin / Q000494:pharmacology; D013696:Temperature; D013752:Tetracycline / Q000494:pharmacology
chemical_list: D000438:Alcohols; D001426:Bacterial Proteins; D002712:Chlorides; D003470:Culture Media; D004269:DNA, Bacterial; D002586:Cesium; D009268:Nalidixic Acid; D011691:Puromycin; D006147:Guanine; D002701:Chloramphenicol; D003596:Cytosine; D013752:Tetracycline; D013307:Streptomycin