Fate of donor deoxyribonucleic acid in a highly transformation-deficient strain of Haemophilus influenzae.
A transformation-deficient strain of Haemophilus influenzae (efficiency of transformation 10(4)-fold less than that of the wild type), designated TD24, was isolated by selection for sensitivity to mitomycin C. In its properties the mutant was equivalent to recA type mutants of Escherichia coli. The TD24 mutation was linked with the str-r marker (about 30%) and only weakly linked with the nov-r2.5 marker. The uptake of donor deoxyribonucleic acid (DNA) was normal in the TD24 strain, but no molecules with recombinant-type activity (molecules carrying both the donor and the resident marker) were formed. In the mutant the intracellular presynaptic fate of the donor DNA was the same as that in the transformation-proficient (wild-type) strain, and the radioactive label of the donor DNA associated covalently with the recipient chromosome in about the same quantity as in the wild type. However, many fewer donor atoms were associated with segments of the mutant's recipient chromosome as compared with segments of the wild-type chromosome. In the mutant the association was accompanied by complete loss of donor marker activity. The lack of donor marker activity of the donor-recipient complex of DNA isolated from the mutant was not due to lack of uptake of the complex by the second recipient and its inability to associate with the second recipient's chromosome. Because the number of donor-atom-carrying resident molecules was higher than could be accounted for by the lengths of presynaptic donor molecules, we favor the idea that the association of donor DNA atoms with the mutant chromosome results from local DNA synthesis rather than from dispersive integration of donor DNA by recombination.
D002499:Centrifugation, Density Gradient; D004269:DNA, Bacterial / Q000032:analysis / Q000378:metabolism*; D004352:Drug Resistance, Microbial; D005092:Exonucleases / Q000378:metabolism; D006193:Haemophilus influenzae / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008937:Mitomycins / Q000494:pharmacology; D009154:Mutation; D009587:Nitrogen Isotopes; D010761:Phosphorus Radioisotopes; D011995:Recombination, Genetic; D013941:Thymine / Q000378:metabolism; D014170:Transformation, Genetic; D014316:Tritium
D004269:DNA, Bacterial; D008937:Mitomycins; D009587:Nitrogen Isotopes; D010761:Phosphorus Radioisotopes; D014316:Tritium; D005092:Exonucleases; D013941:Thymine