Isolation of morphological mutants of Agrobacterium tumefaciens.
Morphological mutants were isolated from a wild strain of Agrobacterium tumefaciens at a high frequency by treatment with a nitrosoguanidine. Seventeen of the 20 mutants isolated were temperature-sensitive. At 27 C, the mutant cells were rod-shaped and at 37 C, spherical or branched, whereas the wild-type cells were rod-shaped at both temperatures.
D003470:Culture Media; D005827:Genetics, Microbial; D008851:Micropore Filters; D008858:Microscopy, Phase-Contrast; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation; D009604:Nitrosoguanidines / Q000494:pharmacology; D012231:Rhizobium / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D013696:Temperature
D003470:Culture Media; D009153:Mutagens; D009604:Nitrosoguanidines