Restriction of the mobility of lymphocyte immunoglobulin receptors by concanavalin A.
The induction by anti-immunoglobulin of patch and cap formation on mouse lymphocytes was inhibited by the addition of concanavalin A. This effect was reversed by alpha-methyl-D-mannoside, a competitive inhibitor of the binding of concanavalin A. Concanavalin A prevented both patch and cap formation, in contrast to the metabolic inhibitor NaN(3), which prevented only cap formation. This suggests that binding of concanavalin A induces changes on or in the lymphocyte membrane that inhibit free diffusion of immunoglobulin receptors.
D000818:Animals; D000937:Antigen-Antibody Reactions / Q000187:drug effects*; D000963:Antimetabolites / Q000494:pharmacology; D001665:Binding Sites; D002462:Cell Membrane / Q000276:immunology; D003208:Concanavalin A / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D007136:Immunoglobulins; D007456:Iodine Isotopes; D037102:Lectins / Q000494:pharmacology*; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008856:Microscopy, Fluorescence; D009584:Nitrogen / Q000494:pharmacology; D011485:Protein Binding; D011955:Receptors, Drug / Q000187:drug effects*; D012964:Sodium / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013696:Temperature
D000963:Antimetabolites; D007136:Immunoglobulins; D007456:Iodine Isotopes; D037102:Lectins; D011955:Receptors, Drug; D003208:Concanavalin A; D012964:Sodium; D009584:Nitrogen