Different blocks in the differentiation of myeloid leukemic cells.
Some clones of mouse myeloid leukemic cells (D(+)) can be induced to undergo cell differentiation to mature macrophages and granulocytes, and other clones (D(-)) could not be induced to differentiate to mature cells. Normal mature macrophages and granulocytes have surface receptors that form rosettes with erythrocytes coated with specific immunoglobulin or immunoglobulin-complement. The D(+) clones were induced to form receptors by prednisolone, cytosine-arabinoside, 5-iododeoxyuridine, actinomycin D, or serum from mice injected with endotoxin. All these compounds thus induced a common change in the cell surface membrane. The induction of receptors required protein synthesis, and receptors were formed before the appearance of mature cells. There were two types of D(-) clones. One type was induced by these compounds to form receptors, although with a lower inducibility than D(+) clones; in the other type there was no induction of receptors. The results indicate that there are different blocks in the differentiation of myeloid leukemic cells. Some leukemic cells (IR(+)D(+)) can be induced to form receptors and to differentiate to mature cells; others (IR(+)D(-)) can form receptors but not mature cells; and a third type (IR(-)D(-)) could not be induced to form receptors or mature cells.
D000818:Animals; D001666:Binding Sites, Antibody; D001769:Blood; D002454:Cell Differentiation; D002462:Cell Membrane / Q000187:drug effects; D002478:Cells, Cultured; D003165:Complement System Proteins; D003561:Cytarabine / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004731:Endotoxins; D004912:Erythrocytes / Q000276:immunology; D006098:Granulocytes / Q000276:immunology*; D007104:Immune Adherence Reaction; D007136:Immunoglobulins; D007942:Leukemia, Experimental / Q000276:immunology; D007951:Leukemia, Myeloid / Q000276:immunology*; D007962:Leukocytes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D011239:Prednisolone / Q000494:pharmacology; D012756:Sheep / Q000276:immunology; D014498:Uracil / Q000031:analogs & derivatives / Q000494:pharmacology
D004731:Endotoxins; D007136:Immunoglobulins; D003561:Cytarabine; D003609:Dactinomycin; D014498:Uracil; D003165:Complement System Proteins; D011239:Prednisolone