Synthesis of Epstein-Barr virus after activation of the viral genome in a "virus-negative" human lymphoblastoid cell (Raji) made resistant to 5-bromodeoxyuridine (thymidine kinase-virus antigen-immunofluorescence-herpesvirus fingerprints).
The Raji line of human lymphoblastoid cells, which does not show expression of Epstein-Barr virus, was made resistant to 5-bromodeoxyuridine. Within several weeks after removal of the drug, Epstein-Barr virus particles were detected in the cells. The sequence of virus appearance in the 5-bromodeoxyuridine-resistant cells indicated drug-induced activation of a repressed Epstein-Barr virus genome.
D000818:Animals; D000956:Antigens, Viral; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003470:Culture Media; D004279:DNA, Viral / Q000096:biosynthesis; D004352:Drug Resistance, Microbial; D005455:Fluorescent Antibody Technique; D005838:Genotype; D006564:Herpesviridae / Q000378:metabolism*; D006801:Humans; D007106:Immune Sera; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008854:Microscopy, Electron; D011817:Rabbits; D013937:Thymidine Kinase / Q000378:metabolism; D013942:Thymine Nucleotides / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects*
D000956:Antigens, Viral; D003470:Culture Media; D004279:DNA, Viral; D007106:Immune Sera; D013942:Thymine Nucleotides; D013937:Thymidine Kinase; D001973:Bromodeoxyuridine