Preparation of purified suspensions of Coxiella burneti by Genetron extraction followed by continuous-flow ultracentrifugation.
A method for the preparation of purified suspensions of Coxiella burneti by Genetron extraction followed by continuous-flow density gradient ultracentrifugation is described. Both phases of the Henzerling strain of C. burneti were found in a zone between 53 and 65% (w/w) sucrose. Based on chemical assays, the Genetron zonal rickettsial suspensions were found to be as pure as the rickettsial suspensions which were prepared by the ether extraction method currently in use for producing Q fever vaccines for human use.
D000818:Animals; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000032:analysis; D001426:Bacterial Proteins / Q000032:analysis; D001428:Bacterial Vaccines / Q000032:analysis; D001431:Bacteriological Techniques; D002501:Centrifugation, Zonal; D002642:Chick Embryo; D002713:Chlorine; D003168:Complement Fixation Tests; D003381:Coxiella / Q000276:immunology / Q000302:isolation & purification*; D005019:Ethyl Ethers; D005321:Extraembryonic Membranes / Q000382:microbiology; D005461:Fluorine; D006168:Guinea Pigs; D006846:Hydrocarbons, Halogenated; D007120:Immunochemistry; D007274:Injections, Intraperitoneal; D008055:Lipids / Q000032:analysis; D009584:Nitrogen / Q000032:analysis; D011778:Q Fever / Q000517:prevention & control; D014461:Ultracentrifugation
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D005019:Ethyl Ethers; D006846:Hydrocarbons, Halogenated; D008055:Lipids; D005461:Fluorine; D002713:Chlorine; D009584:Nitrogen