Isolation of a major cell surface glycoprotein from fibroblasts.
A cell surface component has been isolated in partially purified form from cultured chick embryo and chick heart fibroblasts. This glycoprotein is similar to a protein recently reported to be present at the surface of normal cells, and missing after neoplastic transformation. It is a major cell surface glycoprotein that is synthesized by cultured fibroblasts, but is not collagen. It is shown to be markedly trypsin-sensitive, and its recovery from the cell surface is dependent on cell density. It is excluded from Sephadex G-200, but is not rapidly sedimented by ultracentrifugation, and has an apparent molecular weight of 220,000. The isolation of this cell surface glycoprotein may now provide a means of determining its function.
D000818:Animals; D002462:Cell Membrane / Q000032:analysis*; D002642:Chick Embryo; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000032:analysis*; D006023:Glycoproteins / Q000302:isolation & purification*; D007457:Iodine Radioisotopes; D008970:Molecular Weight; D009206:Myocardium / Q000166:cytology; D012967:Sodium Dodecyl Sulfate; D014357:Trypsin; D014461:Ultracentrifugation
D006023:Glycoproteins; D007457:Iodine Radioisotopes; D012967:Sodium Dodecyl Sulfate; D014357:Trypsin