Rapid enhancement of chick intestinal DNA-dependent RNA polymerase II activity by 1 alpha, 25-dihydroxyvitamin D3, in vivo.
1alpha,25-dihydroxyvitamin D(3) was examined for its ability to affect the DNA-dependent RNA polymerases (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) of rachitic chick intestinal cell nuclei in vivo. Nucleoplasmic (form II) RNA polymerase activity was stimulated 2-fold (P < 0.05) within 2-3 hr after an oral dose of 0.27 mug (0.65 nmol) of 1alpha,25-dihydroxyvitamin D(3) to rachitic chicks. The form II polymerase activity returned to control values by 5-9 hr after dosing with the sterol. In contrast, the nucleolar (form I) RNA polymerase was not increased within this period. Solubilization of nuclear protein and resolution of the two RNA polymerases on DEAE-Sephadex also revealed that there was an increase in polymerase II activity when assayed on exogenous DNA template. This evidence suggests that 1alpha,25-dihydroxyvitamin D(3) acts at the level of the enzymology of intestinal cell transcription and that increased mRNA synthesis after administration of this hormone cannot be due solely to a change in chromatin template activity.
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002467:Cell Nucleus / Q000201:enzymology; D002645:Chickens; D002843:Chromatin; D002852:Chromatography, Ion Exchange; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004100:Dihydroxycholecalciferols / Q000378:metabolism / Q000494:pharmacology; D006887:Hydroxycholecalciferols / Q000494:pharmacology*; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000201:enzymology*; D007421:Intestine, Small / Q000201:enzymology; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis; D012279:Rickets / Q000201:enzymology; D013268:Stimulation, Chemical; D013698:Templates, Genetic; D014158:Transcription, Genetic / Q000187:drug effects; D014316:Tritium
D002843:Chromatin; D004100:Dihydroxycholecalciferols; D006887:Hydroxycholecalciferols; D012333:RNA, Messenger; D014316:Tritium; D012321:DNA-Directed RNA Polymerases; D002118:Calcium