title: Ferredoxin-dependent conversion of acetaldehyde to acetate and H 2  in extracts of S organism.
abstract: S organism, an anaerobic gram-negative rod, which is one of two bacterial species isolated from the culture known as "Methanobacillus omelianskii," ferments ethanol to acetate and H(2). The present study shows that extracts of this organism contain ferredoxin and produce acetate from acetaldehyde via aldehyde: ferredoxin oxidoreductase activity. Electrons generated in the reaction are given off as H(2) by a previously demonstrated ferredoxin-linked hydrogenase system. Extracts were shown to contain good phosphotransacetylase and acetokinase activities, but no mechanism of adenosine triphosphate generation during acetaldehyde conversion to acetate could be detected. No evidence could be obtained for coenzyme A (CoASH) or phosphate requirement or for formation of acetyl CoA or acetyl phosphate.
mesh_terms: D000079:Acetaldehyde / Q000378:metabolism*; D000085:Acetates / Q000096:biosynthesis*; D000217:Acyltransferases / Q000032:analysis; D001151:Arsenic; D001419:Bacteria / Q000201:enzymology* / Q000378:metabolism; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003013:Clostridium / Q000201:enzymology; D003065:Coenzyme A; D004926:Escherichia coli / Q000201:enzymology; D000431:Ethanol / Q000096:biosynthesis; D005288:Ferredoxins; D006859:Hydrogen / Q000378:metabolism*; D008956:Models, Chemical; D010088:Oxidoreductases / Q000032:analysis*; D010756:Phosphoric Acids; D010770:Phosphotransferases / Q000032:analysis; D011773:Pyruvates
chemical_list: D000085:Acetates; D005288:Ferredoxins; D010756:Phosphoric Acids; D011773:Pyruvates; D000431:Ethanol; D006859:Hydrogen; D010088:Oxidoreductases; D000217:Acyltransferases; D010770:Phosphotransferases; D000079:Acetaldehyde; D001151:Arsenic; D003065:Coenzyme A