Conjugational complementation analysis of transfer-deficient mutants of Flac in Escherichia coli.
A series of 102 transfer-deficient (tra(-)) mutants of Flac (84 of which have been previously described) were classified as carrying frameshift, amber, ochre, UGA, and nonsuppressible mutations. Techniques were evolved for using cultures of F prime strains as efficient recipients in matings, for measuring the number of (F'/F') heterozygote cells in transient populations, and for measuring complementation between different Flac tra(-) mutants. These techniques were used to define nine tra cistrons and to assign most of the tra(-) mutations to one or other of these.
D001431:Bacteriological Techniques; D003227:Conjugation, Genetic; D003433:Crosses, Genetic; D003470:Culture Media; D004926:Escherichia coli / Q000145:classification* / Q000254:growth & development / Q000378:metabolism; D005816:Genetic Complementation Test; D005827:Genetics, Microbial; D006579:Heterozygote; D007785:Lactose / Q000378:metabolism; D009153:Mutagens; D009154:Mutation; D010641:Phenotype; D014161:Transduction, Genetic
D003470:Culture Media; D009153:Mutagens; D007785:Lactose