Concentration and purification of influenza virus on insoluble polyelectrolytes.
A method for rapid concentration and purification of influenza virus by adsorption on and elution from an insoluble polyelectrolyte is described. To accomplish this task, influenza virus had to be rendered stable at pH 4 to 5, since viruses adsorb to the polyelectrolyte more efficiently at this pH range. A precipitate which forms in influenza harvests under acid conditions in the cold can be removed by ammonium sulfate at a concentration which traps the precipitate but not the virus. Thus, ammonium sulfate-treated influenza virus in allantoic fluid could be readily concentrated on the polyelectrolyte. Elution yielded a virus concentrate essentially free of nonviral proteins.
D000327:Adsorption; D000645:Ammonium Sulfate; D000818:Animals; D001431:Bacteriological Techniques; D011232:Chemical Precipitation; D002642:Chick Embryo; D003470:Culture Media; D004573:Electrolytes; D004912:Erythrocytes / Q000276:immunology; D006386:Hemagglutination Tests; D006863:Hydrogen-Ion Concentration; D007122:Immunoelectrophoresis; D008722:Methods; D009975:Orthomyxoviridae / Q000276:immunology / Q000302:isolation & purification*; D013552:Swine / Q000276:immunology
D003470:Culture Media; D004573:Electrolytes; D000645:Ammonium Sulfate