title: Bacteriophage T7 DNA replication: a linear replicating intermediate (gradient centrifugation-electron microscopy-E. coli-DNA partial denaturation).
abstract: The T7 chromosome in the first round of replication is a Y-shaped DNA rod. Thus, it differs from previously observed bacterial and viral replicating chromosomes that are circular.
mesh_terms: D002499:Centrifugation, Density Gradient; D002869:Chromosome Aberrations; D002874:Chromosome Mapping; D002875:Chromosomes / Q000378:metabolism; D003090:Coliphages / Q000378:metabolism*; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D004926:Escherichia coli / Q000378:metabolism; D008854:Microscopy, Electron; D009587:Nitrogen Isotopes; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D011995:Recombination, Genetic
chemical_list: D004279:DNA, Viral; D009587:Nitrogen Isotopes