Bacteriophages of Rhodopseudomonas spheroides: isolation and characterization of a Rhodopseudomonas spheroides bacteriophage.
A DNA-containing bacteriophage, designated RS1, infecting Rhodopseudomonas spheroides 2.4.1, has been isolated from sewage. The buoyant density of RS1 in CsCl equilibrium centrifugation is 1.50 g/cm(3), and the buoyant density of RS1 DNA is 1.706. The phage possesses a polyhedral head, approximately 65 nm in diameter, and a tail 60 nm long. When grown on aerobic cells, RS1 has a latent period of 120 min and an average burst size of 20. When grown on anaerobic cells, RS1 has a latent period of 150 min, and a burst size similar to that observed during aerobic infection. The adsorption rate constant of RS1 to aerobic cells is 1.2 x 10(-9) ml/min, and 0.58 x 10(-9) ml/min to anaerobic cells. Adsorption of RS1 to R. spheroides requires the presence of divalent cations.
D000327:Adsorption; D000332:Aerobiosis; D000693:Anaerobiosis; D001435:Bacteriophages / Q000254:growth & development / Q000302:isolation & purification; D002499:Centrifugation, Density Gradient; D004267:DNA Viruses; D004279:DNA, Viral / Q000032:analysis; D008854:Microscopy, Electron; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D009711:Nucleotides / Q000032:analysis; D012242:Rhodobacter sphaeroides; D012722:Sewage; D010948:Viral Plaque Assay
D004279:DNA, Viral; D009711:Nucleotides; D012722:Sewage