Role of phospholipids in the multiple forms of mammalian elongation factor 1.
Evidence is presented that high-molecular-weight aggregates of elongation factor 1 from calf brain and liver contain phospholipids. Sucrose gradient analysis has shown that these high-molecular-weight species can be converted to a low-molecular-weight form after incubation with different phospholipase preparations. In addition, a partially purified elongation factor 1 from rabbit reticulocytes is also converted to a low-molecular-weight species by phospholipase treatment. These data indicate that phospholipids are involved in maintaining the integrity of the high-molecular-weight species and support the view that this species is an aggregate of the low-molecular-weight elongation factor 1.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002499:Centrifugation, Density Gradient; D002784:Cholesterol / Q000378:metabolism; D002850:Chromatography, Gel; D008099:Liver / Q000201:enzymology; D051379:Mice; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000032:analysis; D010445:Peptide Elongation Factors / Q000032:analysis*; D010740:Phospholipases / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis*; D012156:Reticulocytes / Q000032:analysis
D009419:Nerve Tissue Proteins; D010445:Peptide Elongation Factors; D010743:Phospholipids; D002784:Cholesterol; D010740:Phospholipases