Purification of naked intranuclear particles from human liver infected by hepatitis B virus.
The isolation and purification of the naked intranuclear particles of a human liver infected with hepatitis B virus is described. The particles had a diameter of 27-28 nm, were hexagonal, and showed the presence of capsomeres and spikes with lengths of 7-10 nm projecting from the surfaces. The particles formed cubic arrays with center-to-center distances of 40-42 nm. The overall particle symmetry was icosahedral. The ultraviolet absorption spectrum of the particles showed a peak at 264 nm with a shoulder at 280 nm. Endogenous DNA polymerase activity was not detected in fractions containing particles. DNA polymerase activity was present when activated DNA or d(A-T)(n) was used as exogenous template.
D000042:Absorption; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000201:enzymology / Q000382:microbiology*; D002499:Centrifugation, Density Gradient; D004254:DNA Nucleotidyltransferases / Q000032:analysis; D012321:DNA-Directed RNA Polymerases / Q000032:analysis; D006506:Hepatitis A / Q000382:microbiology*; D006511:Hepatitis B Antigens; D006518:Hepatitis Viruses / Q000302:isolation & purification*; D006801:Humans; D008099:Liver / Q000382:microbiology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D011119:Polynucleotides; D013056:Spectrophotometry, Ultraviolet; D013698:Templates, Genetic
D006511:Hepatitis B Antigens; D011119:Polynucleotides; D004254:DNA Nucleotidyltransferases; D012321:DNA-Directed RNA Polymerases