Simple method for the preparation of streptococcal nucleases.
A simple method for preparation and fractionation of the streptococcal nucleases by polyacrylamide gel electrophoresis is presented. The procedure is carried out with ammonium sulfate-precipitated supernatant fluids from cultures of beta-hemolytic streptococci grown to stationary phase. Electrophoresis on polyacrylamide gel and subsequent elution of the fractionated enzymes allows the preparation of sufficient homogeneous nuclease B for a large number of anti-nuclease B titrations.
D000645:Ammonium Sulfate; D011232:Chemical Precipitation; D004247:DNA / Q000378:metabolism; D003851:Deoxyribonucleases / Q000302:isolation & purification* / Q000378:metabolism; D004589:Electrophoresis, Disc; D007202:Indicators and Reagents; D008722:Methods; D008851:Micropore Filters; D013291:Streptococcus / Q000201:enzymology*; D013297:Streptococcus pyogenes / Q000201:enzymology / Q000254:growth & development
D007202:Indicators and Reagents; D004247:DNA; D003851:Deoxyribonucleases; D000645:Ammonium Sulfate