Effects of selenium compounds on formate metabolism and coincidence of selenium-75 incorporation and formic dehydrogenase activity in cell-free preparations of Escherichia coli.
The effects of selenite, selenocystine, and selenomethionine in a defined growth medium on formic dehydrogenase biosynthesis in aerobically and anaerobically grown Escherichia coli have been studied. Sucrose gradient centrifugation of a partially purified enzyme demonstrated a coincidence of (75)Se incorporation and formic dehydrogenase activity.
D000332:Aerobiosis; D000693:Anaerobiosis; D002130:Calcium Phosphates; D002499:Centrifugation, Density Gradient; D003553:Cystine / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D005561:Formates / Q000378:metabolism*; D005782:Gels; D008715:Methionine / Q000494:pharmacology; D008751:Methylene Blue; D010088:Oxidoreductases / Q000096:biosynthesis* / Q000302:isolation & purification; D010269:Paraquat; D011868:Radioisotopes; D012643:Selenium / Q000378:metabolism / Q000494:pharmacology*
D002130:Calcium Phosphates; D005561:Formates; D005782:Gels; D011868:Radioisotopes; D003553:Cystine; D008715:Methionine; D010088:Oxidoreductases; D012643:Selenium; D010269:Paraquat; D008751:Methylene Blue