title: Biosynthesis of thiamine pyrophosphate in Escherichia coli.
abstract: In Escherichia coli, thiamine pyrophosphate is synthesized from thiamine monophosphate. Free thiamine is not involved as an intermediate in de novo synthesis of thiamine pyrophosphate.
mesh_terms: D000332:Aerobiosis; D002247:Carbon Isotopes; D002474:Cell-Free System; D003470:Culture Media; D004926:Escherichia coli / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D005827:Genetics, Microbial; D005947:Glucose / Q000378:metabolism; D009154:Mutation; D010710:Phosphates / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D013831:Thiamine / Q000378:metabolism; D013835:Thiamine Pyrophosphate / Q000096:biosynthesis*
chemical_list: D002247:Carbon Isotopes; D003470:Culture Media; D010710:Phosphates; D010770:Phosphotransferases; D005947:Glucose; D013835:Thiamine Pyrophosphate; D013831:Thiamine