Dissociation and interaction of individual components of a degradative plasmid aggregate in Pseudomonas.
The transfer of the OCT plasmid from Pseudomonas oleovorans to Pseudomonas putida strain PpGl results in the acquisition of three independent replicons: OCT, factor K, and the MER plasmid. OCT is a nontransmissible plasmid harboring genes that code for the enzymes responsible for the degradation of n-octane. Factor K is a transfer plasmid capable of mobilizing OCT as well as chromosomal genes but incapable of enhancing transfer frequencies of other transmissible plasmids such as CAM, SAL, or RP-1. MER is a self-transmissible plasmid which can confer resistance to high concentrations of mercury salts. While OCT and MER are incompatible with CAM, factor K is compatible with it. Transmissible plasmids such as SAL, CAM, MER, or RP-1 cannot mobilize OCT to any significant extent, and exert strong repression on factor K-mediated transfer of chromosomal genes.
D000473:Alkanes / Q000378:metabolism; D001711:Biotransformation; D003227:Conjugation, Genetic; D004352:Drug Resistance, Microbial; D005111:Extrachromosomal Inheritance; D005796:Genes; D008628:Mercury / Q000494:pharmacology*; D010641:Phenotype; D011549:Pseudomonas / Q000378:metabolism*; D014161:Transduction, Genetic
D000473:Alkanes; D008628:Mercury