Deletion map of the Escherichia coli K-12 sex factor F: the order of eleven transfer cistrons.
A series of Hfr deletion mutants was isolated. These mutants contain deletions which extend from a lambda prophage into an Flac which is integrated into the gal operon. Transfer-deficient deletion mutants were found to fall into four different phenotypic groups when tested for male- and female-specific phage resistance. Conjugational and transductional complementation tests with Flac point mutants deficient in transfer (tra(-)) were performed, and the order of 11 tra cistrons was determined. The tra genes are all located between an F gene for the inhibition of female-specific phages and the transposed lac operon originally carried by the Flac. The order of genes in the Hfr studied was established to be: proC... phi(II) (R)... traJ traA traE traK traB traC traF traH traG traD traI...lac...attlambda...bio.
D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003090:Coliphages; D003227:Conjugation, Genetic; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism; D005796:Genes; D005816:Genetic Complementation Test; D006358:Hot Temperature; D007785:Lactose / Q000378:metabolism; D008242:Lysogeny; D009154:Mutation; D009876:Operon; D010641:Phenotype; D012343:RNA, Transfer; D011995:Recombination, Genetic; D014161:Transduction, Genetic
D004269:DNA, Bacterial; D012343:RNA, Transfer; D007785:Lactose