A ribosome dissociation factor from rabbit reticulocytes (fluoride-E. coli ribosomes-initiation factors).
A ribosome dissociation factor has been detected in an extract of ribosomal particles from rabbit reticulocytes. This factor dissociates free ribosomes from reticulocytes and also from Escherichia coli; it does not dissociate ribosomes complexed with peptidyl-tRNA and mRNA. The reaction appears to be stoichiometric rather than catalytic; it reaches completion in one minute at 37 degrees C, but is very slow at 0 degrees C, and it is antagonized and reversed by Mg(++). Reticulocyte dissociation factor thus closely resembles that from E. coli. However, the activity has been found primarily associated with the native large subunits rather than the small subunits in lysates.
D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D004926:Escherichia coli; D005459:Fluorides; D008274:Magnesium; D010442:Peptide Chain Initiation, Translational; D011691:Puromycin; D011817:Rabbits; D012156:Reticulocytes / Q000032:analysis*; D012270:Ribosomes / Q000187:drug effects*; D013696:Temperature
D001798:Blood Proteins; D011691:Puromycin; D008274:Magnesium; D005459:Fluorides