title: Control of 5-aminolaevulinate synthetase activity in Rhodopseudomonas spheroides. The involvement of sulphur metabolism.
abstract: 1. The ;initial' 5-aminolaevulinate synthetase activity, that is the activity observed immediately after cell disruption, in extracts prepared from unharvested semianaerobically grown Rhodopseudomonas spheroides, was twice that observed under the same assay conditions in extracts prepared from harvested cells. 2. The effect of oxygenation of a culture on the ;maximum' aminolaevulinate synthetase activity, that is the activity observed 1h after disruption of harvested cells, is markedly influenced by the contents of the growth medium. Oxygenation of organisms for 1h in the medium in which they have grown produces an 80-90% decrease in maximum activity, whereas similar treatment of organisms resuspended in fresh medium produces less than a 40% decrease. 3. This protective effect of fresh medium is absolutely dependent on the presence of sulphate. When cells are suspended in sulphate-deficient fresh medium, the maximum activity falls by 65-75% even without oxygenation. A high maximum activity is regenerated when sulphate is resupplied. 4. When organisms are oxygenated in the medium in which they have grown, the cellular contents of GSH+GSSG and cysteine+cystine fall very markedly and homolanthionine is formed. Both the fall in aminolaevulinate synthetase activity and the changes in sulphur metabolism are largely prevented by the addition of compounds which stimulate synthesis of cysteine de novo or inhibit the conversion of cysteine S into homocysteine S. 5. The maximum aminolaevulinate synthetase activity was directly proportional to the GSH+GSSG content of all cell preparations. In glutathione-depleted extracts the ;low'-activity enzyme could be re-activated in vitro by the addition of GSH, GSSG, cysteine or cystine, whereas in extracts with a high glutathione content the ;high'-activity enzyme was unaffected by these sulphur compounds. 6. The activation of low-activity enzyme with exogenous sulphur compounds was prevented by excluding air or by adding NADH. Studies with purified enzyme indicate that sulphur compounds do not interact directly with the enzyme, but that their effect is mediated by a number of other endogenous factors.
mesh_terms: D000624:5-Aminolevulinate Synthetase / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism; D002855:Chromatography, Thin Layer; D003066:Coenzyme A Ligases / Q000032:analysis; D003470:Culture Media; D003545:Cysteine / Q000378:metabolism; D004590:Electrophoresis, Paper; D005978:Glutathione / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000494:pharmacology; D012242:Rhodobacter sphaeroides / Q000187:drug effects / Q000201:enzymology*; D013386:Succinates; D013455:Sulfur / Q000378:metabolism*; D013462:Sulfur Radioisotopes; D014462:Ultrafiltration
chemical_list: D000596:Amino Acids; D003470:Culture Media; D013386:Succinates; D013462:Sulfur Radioisotopes; D013455:Sulfur; D000624:5-Aminolevulinate Synthetase; D003066:Coenzyme A Ligases; D005978:Glutathione; D003545:Cysteine; D010100:Oxygen