Efficiency of thymidine incorporation in Escherichia coli B-r as a function of growth rate.
Short-term labeling with radioactive thymidine led to inaccurate estimates of deoxyribonucleic acid synthesis at some growth rates in steady-state cultures of Escherichia coli B/r. Estimates were corrected in chemostat cultures by adding adenosine, a known inhibitor of thymidine phosphorylase.
D000241:Adenosine / Q000494:pharmacology; D002247:Carbon Isotopes; D003124:Colorimetry; D003470:Culture Media; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis; D004926:Escherichia coli / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D008851:Micropore Filters; D010430:Pentosyltransferases / Q000037:antagonists & inhibitors; D013053:Spectrophotometry; D013936:Thymidine / Q000378:metabolism*; D014316:Tritium
D002247:Carbon Isotopes; D003470:Culture Media; D004269:DNA, Bacterial; D014316:Tritium; D010430:Pentosyltransferases; D000241:Adenosine; D013936:Thymidine