title: Primary structure of the ovine hypothalamic luteinizing hormone-releasing factor (LRF) (LH-hypothalamus-LRF-gas chromatography-mass spectrometry-decapeptide-Edman degradation).
abstract: The primary structure of ovine hypothalamic hypophysiotropic luteinizing hormone-releasing factor, LRF, has been established as pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH(2) by hydrolysis of the peptide with chymotrypsin or pyrrolidone-carboxylylpeptidase and by analysis of the products by an Edman-dansylation sequencing technique, as well as by mass spectrometry of the derived phenylthiohydantoins. A decapeptide with the proposed primary structure, prepared by total synthesis, gave the same result on sequencing. The synthetic decapeptide possesses the same biological activities as the native ovine LRF. The amino-acid sequence of ovine LRF is identical to that already published for porcine LRF.
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002247:Carbon Isotopes; D002268:Carboxypeptidases; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D002918:Chymotrypsin; D003619:Dansyl Compounds; D006868:Hydrolysis; D007031:Hypothalamus / Q000032:analysis*; D007986:Luteinizing Hormone / Q000032:analysis*; D013058:Mass Spectrometry; D010443:Peptide Chain Termination, Translational; D010455:Peptides / Q000032:analysis; D010906:Pituitary Hormone-Releasing Hormones / Q000032:analysis*; D012756:Sheep
chemical_list: D000596:Amino Acids; D002247:Carbon Isotopes; D003619:Dansyl Compounds; D010455:Peptides; D010906:Pituitary Hormone-Releasing Hormones; D007986:Luteinizing Hormone; D002268:Carboxypeptidases; D002918:Chymotrypsin