The molecular weight of the major glycoprotein from the human erythrocyte membrane.
The molecular weight of the major glycoprotein from the human erythrocyte membrane is 29,000, of which 55% is carbohydrate and 45% is protein. The binding of sodium dodecyl sulfate to this glycoprotein is anomalous when compared to water soluble proteins and leads to migration rates in sodium dodecyl sulfate-polyacrylamide gels that cannot be interpreted in terms of molecular weight. Anomalous sodium dodecyl sulfate binding may be a general characteristic of many intrinsic membrane proteins even if they are not glycoproteins, and such proteins are likely to have mobilities in sodium dodecyl sulfate-gel electrophoresis that do not correspond to the mobilities of water soluble proteins of identical molecular weight.
D000596:Amino Acids / Q000032:analysis; D002462:Cell Membrane / Q000032:analysis; D004912:Erythrocytes / Q000648:ultrastructure*; D005643:Fucose / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis* / Q000378:metabolism; D006595:Hexosamines / Q000032:analysis; D006601:Hexoses / Q000032:analysis; D006801:Humans; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D012794:Sialic Acids / Q000032:analysis; D012967:Sodium Dodecyl Sulfate
D000596:Amino Acids; D006023:Glycoproteins; D006595:Hexosamines; D006601:Hexoses; D010455:Peptides; D012794:Sialic Acids; D005643:Fucose; D012967:Sodium Dodecyl Sulfate