title: Ligand-induced movement of lymphocyte membrane macromolecules. 3. Relationship between the formation and fate of anti-Ig-surface Ig complexes and cell metabolism.
abstract: Spleen lymphocytes were studied for the movement and interiorization of complexes of anti-Ig-surface Ig. The movement of the complex into a small, compact zone of the cell membrane (forming a cap) was inhibited by drugs that inhibited glycolysis and oxidative phosphorylation, but not by drugs that affected protein synthesis. Dead lymphocytes did not form caps. Freeze-etching techniques revealed that inhibited lymphocytes showed formation of multiple small complexes over the entire cell surface. Inhibitors of glycolysis and of oxidative phosphorylation also inhibited the interiorization and catabolism of radioiodinated anti-Ig. We hypothesize that cross-linking of all the surface Ig triggers the membrane movements that are required to pull the lattice into one zone of the cell.
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex; D001386:Azides / Q000494:pharmacology; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D002462:Cell Membrane / Q000187:drug effects / Q000276:immunology*; D002470:Cell Survival; D003078:Colchicine / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology; D005613:Freeze Etching; D005947:Glucose / Q000494:pharmacology; D006019:Glycolysis / Q000187:drug effects; D007136:Immunoglobulins; D007461:Iodoacetates / Q000494:pharmacology; D008024:Ligands / Q000494:pharmacology; D051379:Mice; D008854:Microscopy, Electron; D009840:Oligomycins / Q000494:pharmacology; D010085:Oxidative Phosphorylation / Q000187:drug effects; D011817:Rabbits / Q000276:immunology; D013154:Spleen
chemical_list: D000936:Antigen-Antibody Complex; D001386:Azides; D004140:Dinitrophenols; D007136:Immunoglobulins; D007461:Iodoacetates; D008024:Ligands; D009840:Oligomycins; D003571:Cytochalasin B; D003513:Cycloheximide; D005947:Glucose; D003078:Colchicine