Voltage clamp experiments of single muscle fibers of Rana pipiens.
A voltage clamp for single muscle fibers has been developed. Stability of the system was achieved when an artificial node was created by enclosing a single muscle fiber in a petroleum jelly seal which served as an analogue of the myelin sheath. Typical voltage clamp records were obtained with large inward transient currents followed by a delayed rectification of the outward currents. These currents looked qualitatively similar when the transverse tubular system was destroyed. Errors in current measurement, especially those due to anomalous rectification, are discussed.
D000200:Action Potentials; D000818:Animals; D001001:Anura; D004594:Electrophysiology / Q000295:instrumentation; D066298:In Vitro Techniques; D008433:Mathematics; D008564:Membrane Potentials; D009132:Muscles / Q000502:physiology*; D009186:Myelin Sheath / Q000502:physiology; D010577:Petrolatum; D011894:Rana pipiens / Q000502:physiology*; D012996:Solutions / Q000502:physiology
D012996:Solutions; D010577:Petrolatum