Regulation of the immune response. 3. Kinetic differences between thymus- and bone marrow- derived lymphocytes in the proliferative response to heterologous erythrocytes.
The kinetics of the in vivo response to SRBC was studied in mouse spleen at both the B cell and T cell levels. The B cell response was assayed by following the appearance of antibody-secreting cells in the spleen using the hemolytic plaque assay. The T cell response was monitored by following the increase in or "priming" of helper activity in the spleen using a quantitative in vitro assay. The role of cellular proliferation in both responses was established with the inhibitor of mitosis, vinblastine. The results show that, although the development of T cell activity precedes that of anti-SRBC PFC by as much as 1 day, T cells lag at least 1 day behind B cells in the onset of cellular proliferation. The evidence suggests either that the helper T cell which proliferates in response to SRBC does so after helping in the initiation of the primary B cell response or that the proliferative T cell response and the initiation of the primary B cell response involve two different subpopulations of T cells.
D000818:Animals; D000917:Antibody Formation; D000941:Antigens; D001853:Bone Marrow / Q000276:immunology*; D001854:Bone Marrow Cells; D002455:Cell Division; D002478:Cells, Cultured; D017707:Erythrocyte Transfusion; D004912:Erythrocytes / Q000276:immunology*; D007700:Kinetics; D051379:Mice; D012756:Sheep / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D014747:Vinblastine / Q000494:pharmacology; D010948:Viral Plaque Assay
D000941:Antigens; D014747:Vinblastine