Effects of acridine orange on the growth of Escherichia coli.
Exposure of Escherichia coli to critical acridine orange (AO) concentrations did not result in loss of viability. However, the deoxyribonucleic acid (DNA) of cells exposed to such agents was rapidly degraded and repolymerized. On the other hand, a bacterium deficient in DNA repair (pol A(1) (-), lacking DNA polymerase) was sensitive to the action of AO. The DNA of such cells was also degraded but it was not repaired.
D000166:Acridines / Q000494:pharmacology*; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D004254:DNA Nucleotidyltransferases; D004260:DNA Repair; D004269:DNA, Bacterial / Q000302:isolation & purification / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D005827:Genetics, Microbial; D007700:Kinetics; D009154:Mutation; D013936:Thymidine / Q000378:metabolism
D000166:Acridines; D002247:Carbon Isotopes; D004269:DNA, Bacterial; D004254:DNA Nucleotidyltransferases; D013936:Thymidine