Determination of actin messenger RNA in cultures of differentiating embryonic chick skeletal muscle.
Cytoplasmic polyadenylylated messenger RNA from differentiated muscle cultures, when incubated in a wheat germ cell-free system, directed the synthesis of a polypeptide indistinguishable from authentic chicken skeletal muscle actin, as judged by mobility on sodium dodecyl sulfate-polyacrylamide gels, tryptic peptide analyses, and biological activity. The synthesis of actin in the cell-free system was used to assay levels of translatable actin mRNA in cultures of fibroblasts, pre- and post-fusion myoblasts, and myoblasts grown under conditions that prevent fusion. In all cases the amount of actin polypeptide synthesized in the cell-free system was proportional to the rate of actin synthesis in the cultures from which the RNA was extracted. It is suggested that actin synthesis is regulated by the actin mRNA content of the cell and that an increase in the cytoplasmic level of translatable actin messenger RNA is mediated by cell fusion rather than by the terminal round of DNA synthesis.
D000199:Actins / Q000096:biosynthesis*; D000227:Adenine Nucleotides; D000818:Animals; D002454:Cell Differentiation; D002474:Cell-Free System; D002478:Cells, Cultured; D002642:Chick Embryo; D002852:Chromatography, Ion Exchange; D003593:Cytoplasm / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts; D007700:Kinetics; D008715:Methionine / Q000378:metabolism; D009132:Muscles / Q000166:cytology / Q000196:embryology; D010452:Peptide Biosynthesis; D010936:Plant Extracts; D011119:Polynucleotides; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D013462:Sulfur Radioisotopes; D014908:Triticum; D014316:Tritium
D000199:Actins; D000227:Adenine Nucleotides; D010936:Plant Extracts; D011119:Polynucleotides; D012333:RNA, Messenger; D013462:Sulfur Radioisotopes; D014316:Tritium; D008715:Methionine