Regulation of bacteriophage T5 development by ColI factors.
The I-type colicinogenic factor ColIb transforms Escherichia coli from a permissive to a nonpermissive host for bacteriophage T5 reproduction by preventing complete expression of the phage genome. T5-infected ColIb(+) cells synthesize only class I (early) phage protein and ribonucleic acid (RNA). Neither phage-specific class II proteins [associated with viral deoxyribonucleic acid (DNA) replication] nor class III proteins (phage structural components) are formed due to the failure of the infected ColIb(+) cells to synthesize class II or class III phage-specific messenger RNA. Comparable studies with T5-infected cells colicinogenic for the related ColIa factor revealed no decrease in the yield of progeny phage although the presence of the ColIa factor leads to a significant reduction in the amount of phage-directed class III protein synthesis.
D000225:Adenine / Q000378:metabolism; D000327:Adsorption; D001345:Autoradiography; D002247:Carbon Isotopes; D003087:Colicins / Q000378:metabolism* / Q000494:pharmacology; D003090:Coliphages / Q000201:enzymology / Q000254:growth & development*; D004254:DNA Nucleotidyltransferases / Q000032:analysis; D004279:DNA, Viral / Q000096:biosynthesis*; D004589:Electrophoresis, Disc; D004926:Escherichia coli; D005111:Extrachromosomal Inheritance; D007930:Leucine / Q000378:metabolism; D010759:Phosphorus Isotopes; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis* / Q000302:isolation & purification; D013936:Thymidine / Q000378:metabolism; D013941:Thymine / Q000378:metabolism; D014316:Tritium; D014498:Uracil / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D014779:Virus Replication / Q000187:drug effects
D002247:Carbon Isotopes; D003087:Colicins; D004279:DNA, Viral; D010759:Phosphorus Isotopes; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium; D014498:Uracil; D004254:DNA Nucleotidyltransferases; D007930:Leucine; D000225:Adenine; D013941:Thymine; D013936:Thymidine