Separation of specific initiation factors involved in the translation of myosin and myoglobin messenger RNAs and the isolation of a new RNA involved in translation.
Two messenger specific factors, phosphocellulose fractions 3 and 4, have been isolated from the initiation factor 3 fraction of red muscle initiation factors by chromatography on phosphocellulose. When added to a reticulocyte cell-free system containing both myoglobin and myosin mRNAs, phosphocellulose fraction 3 is found to specifically stimulate the synthesis of myoglobin while phosphocellulose fraction 4 is found to specifically stimulate the synthesis of myosin. In addition, a new RNA, isolated from the initiation factor 3 fraction, is shown to specifically inhibit the translation of heterologous mRNAs.
D000818:Animals; D002474:Cell-Free System; D002642:Chick Embryo; D002852:Chromatography, Ion Exchange; D005914:Globins / Q000096:biosynthesis; D009132:Muscles; D009211:Myoglobin / Q000096:biosynthesis*; D009218:Myosins / Q000096:biosynthesis*; D010448:Peptide Initiation Factors / Q000302:isolation & purification*; D014176:Protein Biosynthesis; D012313:RNA / Q000302:isolation & purification* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D012156:Reticulocytes
D009211:Myoglobin; D010448:Peptide Initiation Factors; D012333:RNA, Messenger; D012313:RNA; D005914:Globins; D009218:Myosins