title: Preparation of unfixed undemineralized bone sections: the Bright bone cryostat.
abstract: A bone cryostat is described with which cold sections of unfixed, undemineralized calcified tissue can be prepared. The bone cryostat is suitable for the preparation of the sections required for the histochemical and immunofluorescent study of bone, for electron microprobe analysis, and for bone and joint autoradiography with soluble compounds.
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001345:Autoradiography; D001842:Bone and Bones / Q000201:enzymology / Q000473:pathology*; D002356:Cartilage / Q000201:enzymology / Q000473:pathology; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D008854:Microscopy, Electron; D008867:Microtomy / Q000295:instrumentation*; D051381:Rats; D012034:Refrigeration / Q000295:instrumentation
chemical_list: D000469:Alkaline Phosphatase