Escherichia coli RNA-polymerase binding sites on DNA are only 14 base pairs long and are located between sequences that are very rich in AplusT.
E. coli DNA-dependent RNA-polymerase binding sites on DNAs of T5, T7, and lambda coliphages have been isolated according to three different methods in order to analyze the binding sites themselves as well as the nearest neighboring regions. It is shown that the binding sites are regions that are rather rich in G+C, are about 14 base pairs long and are located between DNA sequences highly enriched in A+T. The biological implications of this result are discussed.
D000227:Adenine Nucleotides / Q000302:isolation & purification; D001483:Base Sequence; D001665:Binding Sites; D003090:Coliphages; D004267:DNA Viruses / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004996:Ethidium / Q000378:metabolism; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009711:Nucleotides / Q000302:isolation & purification* / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D013050:Spectrometry, Fluorescence; D013942:Thymine Nucleotides / Q000302:isolation & purification; D014158:Transcription, Genetic
D000227:Adenine Nucleotides; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D009711:Nucleotides; D010761:Phosphorus Radioisotopes; D013942:Thymine Nucleotides; D012321:DNA-Directed RNA Polymerases; D004996:Ethidium