A DNA-protein complex involved in bacteriophage phi chi 174 particle formation.
A varphiX-specific DNA-protein complex has been isolated from varphiX-infected cells. This complex contains infective circular single-stranded DNA, the proteins of the genes F, G, H, and J in the same proportions as in the phage particle, and, in addition, the gene D-protein. The D-protein makes up 12-22% of the protein part of the complex. The sedimentation value of the complex is about 140 S. In vitro, the complex can be converted to the normal 114S phage particle (with a concomitant loss of its D-protein) or to an uninfective 70S particle and a small amount of free single-stranded DNA. The fast sedimenting particle is not associated with membranes.
D002250:Carbon Radioisotopes; D002499:Centrifugation, Density Gradient; D003090:Coliphages / Q000032:analysis*; D004267:DNA Viruses / Q000032:analysis; D004270:DNA, Circular / Q000302:isolation & purification*; D004277:DNA, Single-Stranded / Q000302:isolation & purification*; D004279:DNA, Viral / Q000302:isolation & purification* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007930:Leucine / Q000378:metabolism; D008970:Molecular Weight; D011485:Protein Binding; D012967:Sodium Dodecyl Sulfate; D014316:Tritium; D014764:Viral Proteins / Q000302:isolation & purification* / Q000378:metabolism; D014779:Virus Replication
D002250:Carbon Radioisotopes; D004270:DNA, Circular; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D014764:Viral Proteins; D014316:Tritium; D012967:Sodium Dodecyl Sulfate; D007930:Leucine