Isolation and characterization of a phosphonomycin-resistant mutant of Escherichia coli K-12.
A mutant was isolated from Escherichia coli K-12 which showed increased resistance towards phosphonomycin, a new bactericidal antibiotic recently isolated from strains of Streptomyces. Evidence is presented which suggests that this mutant is resistant to lysis by phosphonomycin because of a lower affinity of phosphoenolpyruvate: uridine diphospho-N-acetylglucosamine enolpyruvyl transferase for this antibiotic. This mutant was also found to be temperature-sensitive in growth. At 42 C mutant cells grew poorly, and the rate of incorporation of (3)H-diaminopimelic acid into trichloroacetic acid-insoluble material was also greatly reduced. Genetic studies indicate that the increased resistance toward phosphonomycin and temperature sensitivity in growth of this mutant are probably the consequences of a single mutation.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002247:Carbon Isotopes; D002473:Cell Wall / Q000378:metabolism; D002854:Chromatography, Paper; D003470:Culture Media; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D005827:Genetics, Microbial; D005947:Glucose / Q000378:metabolism; D005958:Glucosephosphates / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism; D008858:Microscopy, Phase-Contrast; D009154:Mutation; D010728:Phosphoenolpyruvate; D010770:Phosphotransferases / Q000378:metabolism; D010867:Pimelic Acids / Q000378:metabolism; D013696:Temperature; D014316:Tritium
D000900:Anti-Bacterial Agents; D002247:Carbon Isotopes; D003470:Culture Media; D005958:Glucosephosphates; D005994:Glycerophosphates; D010867:Pimelic Acids; D014316:Tritium; D010728:Phosphoenolpyruvate; D010770:Phosphotransferases; D005947:Glucose