Long pyrimidine tracts of L-cell DNA: localization to repeated DNA.
Like HeLa DNA, L-cell DNA contains a significant number of unexpectedly long pyrimidine tracts. 1.3% of the thymidine residues of L-cell DNA are found in these long polypyrimidine tracts. Analysis of L-cell DNA fractions with different rates of reassociation indicates that polypyrimidine tracts are associated with "repeated" DNA. Longer pyrimidine tracts appear to have a higher repetition frequency than shorter tracts, and some may be repeated as many as 5 x 10(4) times in L-cell DNA.
D000818:Animals; D001483:Base Sequence; D002845:Chromatography; D004273:DNA, Neoplasm / Q000032:analysis* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006367:HeLa Cells / Q000032:analysis; D006801:Humans; D006882:Hydroxyapatites; D007739:L Cells / Q000032:analysis*; D051379:Mice; D009691:Nucleic Acid Denaturation; D009695:Nucleic Acid Renaturation; D009841:Oligonucleotides / Q000302:isolation & purification; D011119:Polynucleotides / Q000032:analysis*; D011742:Pyrimidine Nucleotides / Q000032:analysis*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D004273:DNA, Neoplasm; D006882:Hydroxyapatites; D009841:Oligonucleotides; D011119:Polynucleotides; D011742:Pyrimidine Nucleotides; D014316:Tritium; D013936:Thymidine