Classification of thymus-derived and marrow-derived lymphocytes by demonstration of their antigen-binding characteristics.
Antigen-binding cells of T and B origin can readily be determined by quantitating the number of sheep erythrocytes per rosette after glutaraldehyde fixation. The T(1) and T(2) populations have low antigen-binding properties and are very unstable without fixation. The B(1) and B(2) populations are stable and correlate with precursor and secretory cells. Fixation of rosettes permits a sensitive test for studying differentiation of T and B cells.
D000818:Animals; D000921:Antibody-Producing Cells; D000936:Antigen-Antibody Complex; D001853:Bone Marrow / Q000276:immunology*; D001854:Bone Marrow Cells; D006462:Hemolytic Plaque Technique; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D012756:Sheep; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology*
D000936:Antigen-Antibody Complex