Germination and outgrowth of single spores of Saccharomyces cerevisiae viewed by scanning electron and phase-contrast microscopy.
Single spores of Saccharomyces cerevisiae were examined during germination and outgrowth by scanning electron and phase-contrast microscopy. Also determined were changes in cell weight and light absorbance, trehalose utilization, and synthesis of protein and KOH-soluble carbohydrates. These studies reveal that development of the vegetative cell from a spore follows a definite sequence of events involving dramatic physical and chemical modifications. These changes are: initial rapid loss in cellular absorbance followed later by an abrupt gain in absorbance; reduction in cell weight and a subsequent progressive increase; modification of the spore surface with concomitant diminution in refractility; elongation of the cell and augmentation of surface irregularities; rapid decline in trehalose content of the cell accompanied by extensive formation of KOH-soluble carbohydrates; and bud formation.
D002241:Carbohydrates / Q000096:biosynthesis; D003470:Culture Media; D004187:Disaccharides / Q000378:metabolism; D006878:Hydroxides; D008855:Microscopy, Electron, Scanning; D008858:Microscopy, Phase-Contrast; D011188:Potassium; D014176:Protein Biosynthesis; D012440:Saccharomyces / Q000254:growth & development*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D012997:Solvents; D013053:Spectrophotometry; D013170:Spores / Q000254:growth & development*; D013172:Spores, Fungal / Q000166:cytology / Q000254:growth & development / Q000378:metabolism
D002241:Carbohydrates; D003470:Culture Media; D004187:Disaccharides; D006878:Hydroxides; D012997:Solvents; D011188:Potassium