Homogeneous antibody elicited with dinitrophenyl-gramicidin-S (rabbit-isoelectric focusing).
Rabbit antibody to dinitrophenyl hapten has been elicited by hyperimmunization with a bifunctional cyclic decapeptide antigen, di-dinitrophenyl-gramicidin-S. Examination of the induced antibody by isoelectric focusing, and Sipsian analysis of the haptenbinding data, demonstrated that di-dinitrophenyl-gramicidin-S was capable of stimulating and maintaining the production of a biosynthetically homogeneous antibody in the adult animal.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000917:Antibody Formation; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D006241:Haptens; D007114:Immunization; D007456:Iodine Isotopes; D007525:Isoelectric Focusing; D009596:Nitrophenols / Q000494:pharmacology*; D011485:Protein Binding; D011817:Rabbits; D014449:Tyrothricin / Q000494:pharmacology*
D000888:Antibodies, Anti-Idiotypic; D006241:Haptens; D007456:Iodine Isotopes; D009596:Nitrophenols; D014449:Tyrothricin