Fermentation kinetics and continuous process of L-asparaginase production.
For the purpose of obtaining L-asparaginase in quantities from Erwinia aroideae, cell growth and enzyme formation were investigated in both batch and continuous fermentation. Using yeast extract as a growth-limiting substrate, the relationship between specific growth rate and substrate concentration was found to fit the Monod equation. The optimum temperature for enzyme production was 24 C, although cell growth was higher at 28 C. The enzyme yield reached its maximum of 4 IU/ml during the negative acceleration growth phase which occurs just prior to stationary growth. Compared to batch fermentations, the continuous fermentation process gave a lower enzyme yield except when the fermentation was conducted at a dilution rate of 0.1 hr(-1). The graphical method frequently used for prediction of continuous fermentation does not apply to L-asparaginase production by E. aroideae. The optimum temperature for enzyme production in continuous process was 24 C, which was the same as in batch process. Increasing the temperature from 24 to 28 C resulted in a 20% loss of enzyme yield.
D000641:Ammonia / Q000096:biosynthesis; D001215:Asparaginase / Q000096:biosynthesis* / Q000378:metabolism; D001431:Bacteriological Techniques; D002474:Cell-Free System; D003470:Culture Media; D004885:Erwinia / Q000201:enzymology* / Q000254:growth & development; D005285:Fermentation; D007700:Kinetics; D012440:Saccharomyces; D013053:Spectrophotometry; D013237:Stereoisomerism; D013696:Temperature; D013997:Time Factors
D003470:Culture Media; D000641:Ammonia; D001215:Asparaginase