title: Purification of thiamine-binding protein from Escherichia coli.
mesh_terms: D000645:Ammonium Sulfate; D001426:Bacterial Proteins / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D003488:Cyanogen Bromide / Q000494:pharmacology; D003956:Dialysis; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D008970:Molecular Weight; D009154:Mutation; D011485:Protein Binding; D013831:Thiamine / Q000378:metabolism*; D013835:Thiamine Pyrophosphate / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D003488:Cyanogen Bromide; D013835:Thiamine Pyrophosphate; D000645:Ammonium Sulfate; D013831:Thiamine