Ribosomal vaccines. I. Immunogenicity of ribosomal fractions isolated from Salmonella typhimurium and Yersinia pestis.
The immunogenicity of ribosomes and ribosomal subfractions isolated from Yersina pestis and Salmonella typhimurium has been studied. Ribosomes and ribosomal protein isolated from S. typhimurium protected mice against lethal challenge. Ribosomal ribonucleic acid isolated by phenol extraction failed to induce any significant level of protection in mice. None of the ribosomes or ribosomal subfractions isolated from Y. pestis were effective in inducing immunity to lethal challenge. These results suggest that the immunogen of the ribosomal vaccine is protein.
D000818:Animals; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000302:isolation & purification; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D002499:Centrifugation, Density Gradient; D002845:Chromatography; D007114:Immunization; D008297:Male; D051379:Mice; D011402:Pronase; D012329:RNA, Bacterial / Q000302:isolation & purification; D012335:RNA, Ribosomal / Q000302:isolation & purification; D012260:Ribonucleases; D012270:Ribosomes / Q000276:immunology*; D012486:Salmonella typhimurium / Q000166:cytology / Q000276:immunology*; D012967:Sodium Dodecyl Sulfate; D013053:Spectrophotometry; D013395:Sucrose; D013997:Time Factors; D014357:Trypsin; D014461:Ultracentrifugation; D015010:Yersinia pestis / Q000166:cytology / Q000276:immunology*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012967:Sodium Dodecyl Sulfate; D013395:Sucrose; D012260:Ribonucleases; D014357:Trypsin; D011402:Pronase