Caloric catastrophe.
Six solutes known to be actively transported by bacteria were studied with the cell in a "minimum energy" state to determine if sufficient energy were available from cellular stores of ATP to supply the energy necessary to run postulated membrane-situated "pumps." Steady-state cellular concentrations of potassium, calcium, magnesium, leucine, glycine, and alpha-methyl glucoside were determined together with tracer fluxes, oxygen consumption, ATP turnover, and the P:O ratio. From these measurements, it was calculated that the energy supply, 4.20 cal/340 min-g dry wt, fell far short of the energy necessary (28.28 cal/340 min-g dry wt), by classical membrane theory, to operate "pumps."
D000255:Adenosine Triphosphate / Q000378:metabolism; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism; D002463:Cell Membrane Permeability; D004926:Escherichia coli / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D006027:Glycosides / Q000378:metabolism; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D008433:Mathematics; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D010759:Phosphorus Isotopes; D011188:Potassium / Q000378:metabolism
D006027:Glycosides; D010759:Phosphorus Isotopes; D000255:Adenosine Triphosphate; D007930:Leucine; D008274:Magnesium; D005947:Glucose; D011188:Potassium; D002118:Calcium; D005998:Glycine