title: R factor-controlled restriction and modification of deoxyribonucleic acid: restriction mutants.
abstract: Restriction mutants of two different R factor-controlled host specificities (RI and RII) were isolated. All of the restriction mutants examined had a normal modification phenotype. No complementation was observed between the RI and RII host specificities. It is concluded that for each host specificity no protein subunit is shared by the restriction endonuclease and modification methylase.
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003090:Coliphages; D003227:Conjugation, Genetic; D004269:DNA, Bacterial; D004352:Drug Resistance, Microbial; D004720:Endonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology; D005111:Extrachromosomal Inheritance; D005796:Genes; D005816:Genetic Complementation Test; D008780:Methyltransferases / Q000378:metabolism*; D009154:Mutation; D010641:Phenotype; D014161:Transduction, Genetic
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D008780:Methyltransferases; D004720:Endonucleases