Filamentous bacterial viruses. VI. Role of fd gene 2 in deoxyribonucleic acid replication.
Functional gene 2 product was found to be necessary for fd deoxyribonucleic acid (DNA) synthesis throughout the life cycle of the virus. Bacteria which had been infected with a temperature-sensitive gene 2 mutant ceased to make virus-specific DNA when transferred to restrictive conditions at any time after infection, although current rounds of replication were completed.
D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D003090:Coliphages / Q000032:analysis / Q000254:growth & development / Q000378:metabolism*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000032:analysis / Q000096:biosynthesis; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis*; D004926:Escherichia coli / Q000032:analysis / Q000187:drug effects / Q000378:metabolism; D005809:Genes, Regulator; D005827:Genetics, Microbial; D008937:Mitomycins / Q000494:pharmacology; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010756:Phosphoric Acids / Q000378:metabolism; D010759:Phosphorus Isotopes; D013696:Temperature; D013936:Thymidine / Q000378:metabolism; D013941:Thymine / Q000378:metabolism; D014316:Tritium
D002247:Carbon Isotopes; D004269:DNA, Bacterial; D004279:DNA, Viral; D008937:Mitomycins; D010756:Phosphoric Acids; D010759:Phosphorus Isotopes; D014316:Tritium; D013941:Thymine; D013936:Thymidine