Cell wall peptidoglycan mutants of Escherichia coli K-12: existence of two clusters of genes, mra and mrb, for cell wall peptidoglycan biosynthesis.
Temperature-sensitive mutants of Escherichia coli K-12 which cannot form cell wall peptidoglycan at 42 C were isolated. The thermosensitive steps were characterized biochemically, and the genes coding the enzymes of peptidoglycan synthesis were mapped. These genes were in two clusters: one group, located at about 1.5 min between leu and azi, was designated as mra (murein a); the second group, located at about 77.5 min close to argH and metB, was designated as mrb (murein b, with the order metB-argH-mrb). No simple relations were found between the gene location and the order or localization of enzymes involved in the sequence of reactions of cell wall peptidoglycan biosynthesis.
D000409:Alanine / Q000378:metabolism; D002247:Carbon Isotopes; D002268:Carboxypeptidases / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism*; D002474:Cell-Free System; D002854:Chromatography, Paper; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004926:Escherichia coli / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism*; D005944:Glucosamine / Q000378:metabolism; D008025:Ligases / Q000378:metabolism; D009154:Mutation; D010088:Oxidoreductases / Q000378:metabolism; D010453:Peptide Synthases / Q000378:metabolism; D010457:Peptidoglycan / Q000096:biosynthesis*; D010728:Phosphoenolpyruvate; D013237:Stereoisomerism; D013696:Temperature; D014161:Transduction, Genetic; D014166:Transferases / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D002247:Carbon Isotopes; D010457:Peptidoglycan; D010728:Phosphoenolpyruvate; D010088:Oxidoreductases; D014166:Transferases; D002268:Carboxypeptidases; D008025:Ligases; D010453:Peptide Synthases; D005944:Glucosamine; D000409:Alanine; D014529:Uridine