The mechanism of action of methionyl-tRNA synthetase. 3. Ion requirements and kinetic parameters of the ATP-PPi exchange and methionine-transfer reactions catalyzed by the native and trypsin-modified enzymes.
D000215:Acylation; D000255:Adenosine Triphosphate; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D000643:Ammonium Chloride; D002384:Catalysis; D004926:Escherichia coli / Q000201:enzymology*; D007700:Kinetics; D008274:Magnesium; D008715:Methionine; D011189:Potassium Chloride; D011485:Protein Binding; D011487:Protein Conformation; D012343:RNA, Transfer; D013050:Spectrometry, Fluorescence; D013095:Spermidine; D013096:Spermine; D013329:Structure-Activity Relationship; D014357:Trypsin
D000643:Ammonium Chloride; D013096:Spermine; D011189:Potassium Chloride; D000255:Adenosine Triphosphate; D012343:RNA, Transfer; D008715:Methionine; D014357:Trypsin; D000604:Amino Acyl-tRNA Synthetases; D008274:Magnesium; D013095:Spermidine