Purification of Escherichia coli alkaline phosphatase. Improved growth conditions for the bacteria, modified methods of preparation of the enzyme.
D000042:Absorption; D000469:Alkaline Phosphatase / Q000302:isolation & purification*; D000596:Amino Acids / Q000032:analysis; D001426:Bacterial Proteins / Q000032:analysis; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004589:Electrophoresis, Disc; D004789:Enzyme Activation; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development; D008722:Methods; D013997:Time Factors; D014461:Ultracentrifugation; D015032:Zinc / Q000032:analysis
D000596:Amino Acids; D001426:Bacterial Proteins; D000469:Alkaline Phosphatase; D015032:Zinc