Filamentous bacterial viruses. VII. Inhibition of fd deoxyribonucleic acid synthesis after a temperature jump into protein-synthesis inhibitors.
Synthesis of fd deoxyribonucleic acid (DNA) was stopped by transferring infected bacteria from 32 C into chloramphenicol or serine hydroxamate at 42 C, but not by addition of these antibiotics at 32 C, and not by a temperature change in the absence of antibiotics. The inhibition of fd DNA synthesis by serine hydroxamate at 42 C was reversed by excess serine. The ability to synthesize fd DNA at 42 C in chloramphenicol was rescued by delaying the addition of chloramphenicol for a few minutes after the transfer from 32 to 42 C. The colony-forming ability of abortively infected bacteria was also rescued from "killing" by delaying the addition of chloramphenicol after a transfer from 32 to 42 C.
D002499:Centrifugation, Density Gradient; D002701:Chloramphenicol / Q000494:pharmacology*; D003090:Coliphages / Q000032:analysis / Q000254:growth & development / Q000378:metabolism*; D004261:DNA Replication / Q000187:drug effects; D004269:DNA, Bacterial / Q000032:analysis / Q000096:biosynthesis; D004279:DNA, Viral / Q000032:analysis / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D004926:Escherichia coli / Q000032:analysis / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D006877:Hydroxamic Acids / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism; D008937:Mitomycins / Q000494:pharmacology; D010756:Phosphoric Acids / Q000378:metabolism; D010759:Phosphorus Isotopes; D012694:Serine / Q000494:pharmacology*; D013395:Sucrose; D013696:Temperature; D013941:Thymine / Q000378:metabolism; D014316:Tritium
D004269:DNA, Bacterial; D004279:DNA, Viral; D006877:Hydroxamic Acids; D008937:Mitomycins; D010756:Phosphoric Acids; D010759:Phosphorus Isotopes; D014316:Tritium; D012694:Serine; D013395:Sucrose; D002701:Chloramphenicol; D007930:Leucine; D013941:Thymine