A scheme for the preliminary fractionation of E. coli extracts for the large-scale isolation of tryptophanyl- and lysyl-tRNA-synthetases and tRNA.
D000818:Animals; D002247:Carbon Isotopes; D002417:Cattle; D002848:Chromatography, DEAE-Cellulose; D003851:Deoxyribonucleases; D004926:Escherichia coli / Q000032:analysis* / Q000201:enzymology; D008239:Lysine; D008722:Methods; D010179:Pancreas / Q000201:enzymology; D010759:Phosphorus Isotopes; D012329:RNA, Bacterial / Q000302:isolation & purification*; D012343:RNA, Transfer / Q000302:isolation & purification*; D014364:Tryptophan
D002247:Carbon Isotopes; D010759:Phosphorus Isotopes; D012329:RNA, Bacterial; D014364:Tryptophan; D012343:RNA, Transfer; D003851:Deoxyribonucleases; D008239:Lysine