Accumulation of methylglyoxal in a mutant of Escherichia coli constitutive for gluconate catabolism.
A culture of a mutant of Escherichia coli, derepressed for gluconate catabolism, is killed by the addition of gluconate to the culture. The product responsible for this bactericidal effect was identified as methylglyoxal. Two types of mutants resistant to gluconate were isolated. One of them showed increased activity of glyoxalase I.
D002474:Cell-Free System; D003470:Culture Media; D004794:Enzyme Repression; D004926:Escherichia coli / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D005942:Gluconates / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006038:Glyoxylates / Q000096:biosynthesis*; D008190:Lyases / Q000378:metabolism; D008745:Methylation; D008851:Micropore Filters; D009154:Mutation; D010770:Phosphotransferases / Q000378:metabolism; D011773:Pyruvates / Q000096:biosynthesis; D013053:Spectrophotometry
D003470:Culture Media; D005942:Gluconates; D006038:Glyoxylates; D011773:Pyruvates; D010770:Phosphotransferases; D008190:Lyases; D005947:Glucose