title: In vivo production of an RNA-DNA copolymer after infection of Escherichia coli by bacteriophage T4.
abstract: An RNA-DNA copolymer was isolated from Escherichia coli infected with bacteriophage T4. The RNA and DNA are covalently linked, and in the same polynucleotide strand. The DNA of the copolymer hybridizes specifically to the left strand of phage T4 DNA. The copolymer is produced in cells infected with amber mutants of phage T4 deficient in DNA replication and is not inhibited by the addition of chloramphenicol.
mesh_terms: D001973:Bromodeoxyuridine / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D002701:Chloramphenicol / Q000494:pharmacology; D003090:Coliphages / Q000187:drug effects / Q000378:metabolism*; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D006868:Hydrolysis; D008242:Lysogeny; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010759:Phosphorus Isotopes; D012367:RNA, Viral / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
chemical_list: D004279:DNA, Viral; D010759:Phosphorus Isotopes; D012367:RNA, Viral; D014316:Tritium; D002701:Chloramphenicol; D003851:Deoxyribonucleases; D012260:Ribonucleases; D001973:Bromodeoxyuridine; D013936:Thymidine