title: Yeast inorganic pyrophosphatase. IV. Purification, quaternary structure, and evidence for strongly bound Mg 2+1 .
mesh_terms: D001665:Binding Sites; D002614:Chelating Agents; D002848:Chromatography, DEAE-Cellulose; D003460:Crystallization; D004229:Dithiothreitol; D004591:Electrophoresis, Polyacrylamide Gel; D008274:Magnesium; D008970:Molecular Weight; D010710:Phosphates; D011485:Protein Binding; D011487:Protein Conformation; D011489:Protein Denaturation; D011755:Pyrophosphatases / Q000037:antagonists & inhibitors / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013054:Spectrophotometry, Atomic; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013997:Time Factors; D015032:Zinc
chemical_list: D002614:Chelating Agents; D010710:Phosphates; D011755:Pyrophosphatases; D008274:Magnesium; D015032:Zinc; D004229:Dithiothreitol