Studies on the high-sulphur proteins of reduced Merino wool. Amino acid sequence of protein SCMKB-3A3.
The complete amino acid sequences of wool protein SCMKB-IIIA3 (131 residues) and a minor component SCMKB-IIIA3A (130 residues) have been determined. The proteins are mutually homologous and have free threonine as the N-terminal residue and carboxymethylcysteine as the C-terminus. The peptides used for the sequence work were obtained by trypsin, thermolysin, pepsin and chymotrypsin digestions and were fractionated by chromatography on DEAE-cellulose, gel filtration on Sephadex G-25 and G-50, paper chromatography and electrophoresis. The Edman degradation method (employing both the Beckman Sequencer and a non-automatic procedure) was used to obtain the sequences of the peptides.
D000595:Amino Acid Sequence; D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D002918:Chymotrypsin; D004590:Electrophoresis, Paper; D066298:In Vitro Techniques; D010434:Pepsin A; D011506:Proteins / Q000032:analysis*; D012756:Sheep; D013820:Thermolysin; D013912:Threonine / Q000032:analysis; D014357:Trypsin; D014935:Wool / Q000032:analysis*
D011506:Proteins; D013912:Threonine; D002918:Chymotrypsin; D014357:Trypsin; D010434:Pepsin A; D013820:Thermolysin