New device for preparing thin slices of adipose tissue for metabolic studies in vitro.
A new microtome is described which allows the rapid preparation of equal slices of well-defined thickness of fresh human tissue, especially adipose tissue. Presetting the microtome for a section thickness of 500 micro m, we found a variation of about 5% with human adipose tissue. Slices of human adipose tissue sliced by the microtome showed a higher sensitivity to insulin and a better reproducibility of results than slices prepared freehand.
D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D002247:Carbon Isotopes; D003584:Cytological Techniques; D005947:Glucose / Q000378:metabolism; D006801:Humans; D008053:Lipid Mobilization; D008055:Lipids / Q000096:biosynthesis; D008867:Microtomy / Q000295:instrumentation*
D002247:Carbon Isotopes; D008055:Lipids; D005947:Glucose