Modification of ribosomes in cryptopleurine-resistant mutants of yeast.
Cryptopleurine-resistant mutants of Saccharomyces cerevisiae were isolated. A single, recessive nuclear gene, very closely linked to the mating locus (2.1 centimorgans), is responsible for resistance. Ribosomes from the mutants were found to be resistant to cryptopleurine when analyzed by poly(U)-directed polyphenylalanine synthesis. Analysis of the distribution of ribosomes between monosomes and polysomes in sensitive cells exposed to cryptopleurine suggests that some step is inhibited during the elongation phase of protein synthesis.
D000470:Alkaloids / Q000494:pharmacology*; D002874:Chromosome Mapping; D003470:Culture Media; D004352:Drug Resistance, Microbial; D005656:Fungal Proteins / Q000096:biosynthesis; D005808:Genes, Recessive; D005827:Genetics, Microbial; D009154:Mutation; D010452:Peptide Biosynthesis; D010445:Peptide Elongation Factors / Q000378:metabolism; D010616:Phenanthrenes / Q000494:pharmacology; D010649:Phenylalanine / Q000378:metabolism; D011807:Quinolizines / Q000494:pharmacology*; D012343:RNA, Transfer / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D013997:Time Factors; D014316:Tritium
D000470:Alkaloids; D003470:Culture Media; D005656:Fungal Proteins; D010445:Peptide Elongation Factors; D010616:Phenanthrenes; D011807:Quinolizines; D014316:Tritium; D010649:Phenylalanine; D012343:RNA, Transfer