title: A genetic method for determining the order of events in a biological pathway.
abstract: We describe the application of a simple genetic test that indicates the order of events in a morphogenic or biochemical pathway. The test uses temperature-sensitive (ts) and cold-sensitive (cs) mutants, and it orders the times at which the ts and cs defects are expressed.
mesh_terms: D000483:Alleles; D002874:Chromosome Mapping; D005827:Genetics, Microbial; D007700:Kinetics; D008242:Lysogeny; D009024:Morphogenesis; D009154:Mutation; D011995:Recombination, Genetic; D012483:Salmonella Phages / Q000254:growth & development* / Q000378:metabolism; D012486:Salmonella typhimurium; D013696:Temperature