Rapid, direct fluorescent-antibody method for the detection of salmonellae in food and feeds.
An improved immunofluorescent-antibody (FA) method for the detection of salmonellae in foods and feeds was developed. This FA method combines a rapid cultural phase and a serological phase that allow for propagation of salmonellae in a minimum time, employing the industrial 8-hr work day as a guide. Two hundred fifty naturally contaminated human food and animal feed samples, representing 647 trials, were tested by the FA method. A total of 18 different food and feed samples was used. The method used by the Association of Official Analytical Chemists (AOAC) for the detection of salmonellae was the control method. The percent agreement when comparing the FA slide method to the AOAC method ranged from 87.1 to 95.3%, depending upon the conjugated antisera used in comparative studies.
D000821:Animal Feed; D000942:Antigens, Bacterial / Q000032:analysis; D003470:Culture Media; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D005506:Food Contamination; D005516:Food Microbiology; D007106:Immune Sera; D008722:Methods; D008856:Microscopy, Fluorescence; D012475:Salmonella / Q000276:immunology / Q000302:isolation & purification*; D012486:Salmonella typhimurium / Q000276:immunology / Q000302:isolation & purification; D013997:Time Factors
D000942:Antigens, Bacterial; D003470:Culture Media; D007106:Immune Sera