Defective deoxyribonucleic acid replication of T4rII bacteriophage in lambda-lysogenic host cells.
Sedimentation of the replicative deoxyribonucleic acid through alkaline sucrose gradients showed that rII single chains reached the half-mature size at a time when wild-type molecules formed long chains (dimers and trimers of genome size). Long rII single chains could be observed on substitution of tris(hydroxymethyl)aminomethane buffer for Na(+)K(+) phosphate in the growth medium.
D002021:Buffers; D002499:Centrifugation, Density Gradient; D003090:Coliphages / Q000378:metabolism*; D003470:Culture Media; D004261:DNA Replication; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D008242:Lysogeny; D009154:Mutation; D010710:Phosphates; D011188:Potassium / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014325:Tromethamine
D002021:Buffers; D003470:Culture Media; D004279:DNA, Viral; D010710:Phosphates; D014325:Tromethamine; D014316:Tritium; D012964:Sodium; D011188:Potassium; D013936:Thymidine