Deletion mapping of the genes coding for HPr and enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system in Salmonella typhimurium.
Sugars transported by a bacterial phosphoenolpyruvate:sugar phosphotransferase system (PTS) require two soluble proteins: HPr, a low-molecular-weight phosphate-carrier protein, and enzyme I. The structural genes coding for HPr (ptsH) and Enzyme I (ptsI) are shown to be cotransducible in Salmonella typhimurium. The gene order of this region of the Salmonella chromosome is cysA-trzA-ptsH-ptsI...(crr). A method for the isolation of trzA-pts deletion is described. One class of pts deletions extends through ptsH and into ptsI; a second class includes both ptsH and ptsI and extends into or through the crr gene. The crr gene either codes for or regulates the synthesis of a third PTS protein (factor III) which is sugar-specific. A hypothesis is presented for a mechanism of deletion formation.
D001426:Bacterial Proteins / Q000096:biosynthesis*; D050260:Carbohydrate Metabolism; D002247:Carbon Isotopes; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004352:Drug Resistance, Microbial; D005796:Genes; D005815:Genetic Code; D005838:Genotype; D009154:Mutation; D010085:Oxidative Phosphorylation; D010641:Phenotype; D010728:Phosphoenolpyruvate / Q000378:metabolism; D010770:Phosphotransferases / Q000096:biosynthesis* / Q000378:metabolism; D011995:Recombination, Genetic; D012483:Salmonella Phages; D012486:Salmonella typhimurium / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D014161:Transduction, Genetic; D014230:Triazoles / Q000494:pharmacology
D001426:Bacterial Proteins; D002247:Carbon Isotopes; D014230:Triazoles; D010728:Phosphoenolpyruvate; D010770:Phosphotransferases