Deletion mutants of bacteriophage phiX174.
Mutants of bacteriophage varphiX174 have been isolated that are less dense than wild-type varphiX particles in CsCl. When mutant viral (+) strand DNA and wild-type complementary (-) strand DNA are hybridized, the resulting duplex molecules have single-stranded loops characteristic of wild-type-deletion heteroduplexes. The mutant bacteriophages fail to complement varphiX amber mutants in cistron E. We conclude that the mutant viruses have deleted approximately 7% of the varphiX genome in the region of cistron E.
D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D003090:Coliphages; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004589:Electrophoresis, Disc; D004926:Escherichia coli / Q000187:drug effects; D005827:Genetics, Microbial; D006378:Helper Viruses; D008433:Mathematics; D008854:Microscopy, Electron; D008937:Mitomycins / Q000494:pharmacology; D009154:Mutation; D009693:Nucleic Acid Hybridization; D014316:Tritium; D014779:Virus Replication
D002247:Carbon Isotopes; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D008937:Mitomycins; D014316:Tritium