Association of lysozyme with ribosomes and ribosome precursor particles.
Lysozyme, used to prepare osmotically fragile cells, combines with ribosomal subunits unless salt is present in excess of 50 mM. The binding of lysozyme to precursor ribonucleoprotein particles can result in their conversion to particles which sediment as mature ribosomes.
D002499:Centrifugation, Density Gradient; D004926:Escherichia coli / Q000166:cytology / Q000201:enzymology; D007457:Iodine Radioisotopes; D009113:Muramidase / Q000378:metabolism*; D009698:Nucleoproteins / Q000378:metabolism; D009996:Osmotic Fragility; D012270:Ribosomes / Q000201:enzymology*
D007457:Iodine Radioisotopes; D009698:Nucleoproteins; D009113:Muramidase