title: Binding of bacteriophage P22 tail parts to cells.
abstract: Purified base-plate parts of bacteriophage P22 can bind to the host cell, Salmonella typhimurium. Although the reaction is reversible, a stable equilibrium is not formed between bound and unbound base-plate parts. This is because the binding sites on the cell, presumably the O antigens, are destroyed. The destruction of binding sites does not kill the cells, and, in fact, the binding sites are soon regenerated. The site-destroying activity reacts with P22 heads to make active phage and with antiserum made against purified phage. Therefore site-destroying activity is a characteristic of the base-plate parts and not some contaminant of the preparation.
mesh_terms: D000327:Adsorption; D000645:Ammonium Sulfate; D001665:Binding Sites / Q000187:drug effects; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D004926:Escherichia coli; D007106:Immune Sera / Q000494:pharmacology; D012483:Salmonella Phages; D012486:Salmonella typhimurium; D014732:Vibration; D014764:Viral Proteins / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D007106:Immune Sera; D014764:Viral Proteins; D000645:Ammonium Sulfate