Effect of pH on adenine and amino acid uptake during sporulation in Saccharomyces cerevisiae.
During the early stages of sporulation in Saccharomyces cerevisiae, the pH of the acetate sporulation medium rises to values of 8.0 or higher. Associated with this rise in pH is a reduced cell permeability to certain precursors of ribonucleic acid (RNA), deoxyribonucleic acid or protein. Uptake of adenine, alanine, and leucine was optimal at pH 5.6 to 6.0, but sporulation was inhibited when the sporulation medium was buffered below pH 7.0. Cellular impermeability can be largely overcome by adjusting the acetate sporulation medium to pH 6.0 for optimal uptake of (14) C-adenine during short pulses without any apparent effect on sporulation. Sporulating cells pulse-labeled 20 min at pH 6.0 incorporated 40 times more (14)C-adenine into RNA than sporulating cells pulse-labeled at pH 8.0. This increased incorporation can be attributed to a 100-fold increase in labeled adenosine triphosphate in cells pulse-labeled at pH 6.0 where maximum uptake occurs.
D000085:Acetates / Q000378:metabolism; D000225:Adenine / Q000378:metabolism*; D000227:Adenine Nucleotides / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000409:Alanine / Q000378:metabolism*; D002247:Carbon Isotopes; D002463:Cell Membrane Permeability; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D007930:Leucine / Q000378:metabolism*; D008540:Meiosis; D010710:Phosphates / Q000378:metabolism; D010759:Phosphorus Isotopes; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D013172:Spores, Fungal / Q000254:growth & development; D013997:Time Factors
D000085:Acetates; D000227:Adenine Nucleotides; D002247:Carbon Isotopes; D003470:Culture Media; D010710:Phosphates; D010759:Phosphorus Isotopes; D012313:RNA; D000255:Adenosine Triphosphate; D007930:Leucine; D000225:Adenine; D000409:Alanine