title: Initiation of deoxyribonucleic acid replication in Escherichia coli B-r: chronology of events and transcriptional control of initiation.
abstract: Three processes necessary for the initiation of deoxyribonucleic acid replication have been separated in time in synchronously growing Escherichia coli B/r. One can be inhibited with 2 mug of chloramphenicol per ml and occurs about 20 min prior to initiation. A second step, occurring 5 to 10 min prior to initiation, is sensitive to 4 to 30 mug of chloramphenicol per ml. A third process occurs at the time of initiation and can be inhibited with rifampin. The experiments suggest that transcription itself, or the synthesis of a relatively small ribonucleic acid, is required for initiation of replication.
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis; D002701:Chloramphenicol / Q000494:pharmacology; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D005827:Genetics, Microbial; D012293:Rifampin / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014158:Transcription, Genetic; D014316:Tritium
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D014316:Tritium; D002701:Chloramphenicol; D013936:Thymidine; D012293:Rifampin