Growth in high osmotic medium of an unsaturated fatty acid auxotroph of Escherichia coli K-12.
A temperature-sensitive mutant of Escherichia coli K-12, which is impaired in the biosynthesis of the unsaturated fatty acids at 42 C, continues to grow and remains viable in a medium with a high osmotic pressure. Under these growth conditions, 50% of the fatty acids of the parental strain and 80% of the fatty acids of the mutant strain are saturated.
D000085:Acetates / Q000378:metabolism; D002250:Carbon Radioisotopes; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D003470:Culture Media; D004926:Escherichia coli / Q000032:analysis / Q000254:growth & development* / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis / Q000096:biosynthesis; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis / Q000378:metabolism*; D008055:Lipids / Q000302:isolation & purification; D009154:Mutation; D009997:Osmotic Pressure; D012965:Sodium Chloride; D013696:Temperature
D000085:Acetates; D002250:Carbon Radioisotopes; D003470:Culture Media; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D008055:Lipids; D012965:Sodium Chloride