Comparison of the activities of extracts of Escherichia coli and Salmonella typhimurium in amino acid incorporation.
We have compared the amino acid incorporating activities of extracts of Escherichia coli and Salmonella typhimurium in in vitro protein-synthesizing systems directed by bacterial messenger ribonucleic acid (mRNA) of both species and by the genomes of coliphages Qbeta and f2. E. coli and S. typhimurium extracts translate both homologous and heterologous bacterial mRNAs at comparable rates. S. typhimurium extracts translate phage RNAs only 10 to 15% as fast as E. coli extracts do. The presence of glucose in the growth medium increases the activity of S. typhimurium extracts three- to fourfold in the phage RNA-directed systems. Glucose has a much more limited effect on the activities of E. coli extracts. We show that similar amounts of phage RNA-ribosome complexes are formed in both the E. coli and the S. typhimurium systems, indicating that the different activities observed may be attributed to different rates of peptide elongation or to the formation of complexes at different sites on the RNA strand.
D000596:Amino Acids / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis; D002021:Buffers; D002250:Carbon Radioisotopes; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D003090:Coliphages / Q000378:metabolism; D003470:Culture Media; D004926:Escherichia coli / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D010441:Peptide Chain Elongation, Translational; D012329:RNA, Bacterial / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D012486:Salmonella typhimurium / Q000378:metabolism*; D013395:Sucrose; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D000596:Amino Acids; D001426:Bacterial Proteins; D002021:Buffers; D002250:Carbon Radioisotopes; D003470:Culture Media; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012367:RNA, Viral; D014316:Tritium; D013395:Sucrose; D007930:Leucine; D005947:Glucose; D014529:Uridine