Induction of B cell tolerance in vitro to 2,4-dinitrophenyl coupled to a copolymer of D-glutamic acid and D-lysine (DNP-D-GL).
Spleen cells from CBA or congenitally athymic ("nude") mice were pretreated with various concentrations of DNP coupled to a copolymer of D-glutamic acid and D-lysine (DNP(37)-D-GL), under various conditions of time and temperature. After washing, they were then cultured for 3 days with the direct B cell immunogen, DNP coupled to Salmonella adelaide flagella (DNP-FLA). Under all circumstances tried, exposure of cells to 1 microg/ml DNP-D-GL caused a 70-100% depression in the subsequent DNP-specific PFC response, and 100 ng/ml caused a lesser but still substantial effect. At the concentrations used, DNP-D-GL did not affect irrelevant antibody responses. Though cells from nude mice responded somewhat less well to DNP-FLA than those from CBA mice, no significant difference in the reaction of the two populations to the tolerogen was noted. This demonstrates that DNP-D-GL can, as previously suspected, directly cause unresponsiveness in B lymphocytes.
D000818:Animals; D000921:Antibody-Producing Cells; D000942:Antigens, Bacterial; D001402:B-Lymphocytes / Q000276:immunology*; D001666:Binding Sites, Antibody; D002478:Cells, Cultured; D004140:Dinitrophenols; D005407:Flagella / Q000276:immunology; D005971:Glutamates; D006241:Haptens; D006462:Hemolytic Plaque Technique; D007108:Immune Tolerance; D008239:Lysine; D008297:Male; D051379:Mice; D012475:Salmonella / Q000276:immunology; D013154:Spleen / Q000276:immunology
D000942:Antigens, Bacterial; D004140:Dinitrophenols; D005971:Glutamates; D006241:Haptens; D008239:Lysine