Accessibility of DNA in chromatin to DNA polymerase and RNA polymerase.
The accessibility of DNA in chromatin to both exogenous DNA polymerase and RNA polymerase is slight when compared to isolated DNA. DNA in extracted chromatin is somewhat more accessible to these enzymes than is DNA in the chromatin of isolated nuclei; and the DNA template of chromatin is more accessible to DNA polymerase than to RNA polymerase. In these experiments we have given much attention to the technique of scintillation counting, since artifacts arising in this procedure can lead to erroneous conclusions.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002122:Calcium Chloride; D002417:Cattle; D002467:Cell Nucleus / Q000032:analysis; D002499:Centrifugation, Density Gradient; D002843:Chromatin / Q000032:analysis / Q000378:metabolism*; D003597:Cytosine Nucleotides / Q000378:metabolism; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism*; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004261:DNA Replication; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D005374:Filtration; D006160:Guanosine Triphosphate / Q000378:metabolism; D008722:Methods; D013698:Templates, Genetic; D013942:Thymine Nucleotides / Q000378:metabolism; D013950:Thymus Gland / Q000032:analysis; D014316:Tritium; D014500:Uracil Nucleotides / Q000378:metabolism
D002843:Chromatin; D003597:Cytosine Nucleotides; D013942:Thymine Nucleotides; D014500:Uracil Nucleotides; D014316:Tritium; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D004247:DNA; D004254:DNA Nucleotidyltransferases; D012321:DNA-Directed RNA Polymerases; D002122:Calcium Chloride