Lambda phage DNA: joining of a chemically synthesized cohesive end.
The chemically synthesized dodecamer d(pA-G-G-T-C-G-C-C-G-C-C-C) was annealed, and was covalently joined to lambda phage DNA with bacterio-phage T(4) ligase. The 5'-end of the dodecamer was joined to a deoxyguanosine residue. Repair with DNA polymerase I established that the position of joining was the left-hand end of lambda DNA. This is the first time that a chemically synthesized oligonucleotide has been covalently joined to a naturally occurring DNA molecule.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D001483:Base Sequence; D002848:Chromatography, DEAE-Cellulose; D003090:Coliphages / Q000032:analysis* / Q000201:enzymology; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D004260:DNA Repair; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D008025:Ligases / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010759:Phosphorus Isotopes; D011119:Polynucleotides / Q000032:analysis / Q000138:chemical synthesis* / Q000378:metabolism
D004279:DNA, Viral; D010759:Phosphorus Isotopes; D011119:Polynucleotides; D000255:Adenosine Triphosphate; D004254:DNA Nucleotidyltransferases; D000469:Alkaline Phosphatase; D008025:Ligases