title: Evidence for an extrakaryotic mutation affecting the maintenance of the rho factor in yeast.
abstract: A newly isolated, temperature-sensitive mutant of a haploid strain of Saccharomyces cerevisiae is described. Its shift to nonpermissive temperature (35 C) resulted in an irreversible change to rho(-), causing, within four to six generations, more than 90% of the cells to form petite colonies. Genetic analysis revealed extrakaryotic inheritance of this temperature-sensitive mutation. Data presented indicate mutation of a gene in the mitochondrial deoxyribonucleic acid affecting the maintenance of the rho factor.
mesh_terms: D003433:Crosses, Genetic; D003470:Culture Media; D004272:DNA, Mitochondrial; D005111:Extrachromosomal Inheritance; D005796:Genes; D005947:Glucose; D005990:Glycerol; D006238:Haploidy; D008938:Mitosis; D009153:Mutagens; D009154:Mutation; D009604:Nitrosoguanidines; D012441:Saccharomyces cerevisiae / Q000254:growth & development* / Q000302:isolation & purification; D013172:Spores, Fungal / Q000254:growth & development; D013696:Temperature
chemical_list: D003470:Culture Media; D004272:DNA, Mitochondrial; D009153:Mutagens; D009604:Nitrosoguanidines; D005947:Glucose; D005990:Glycerol