title: Location of the origin of transfer of the sex factor F.
abstract: The location of the origin of transfer on the map of Flac has been determined by two different techniques. The first showed that no transfer cistrons were transferred early by a transposition Hfr strain with a known point of chromosomal integration into Flac, and the second used a series of transfer-deficient Hfr deletion strains to map a locus required in cis for chromosome transfer by an incoming Flac factor. Both techniques gave results consistent with a location for the origin of transfer between traJ and the phi(R) (11) locus.
mesh_terms: D000483:Alleles; D001483:Base Sequence; D002876:Chromosomes, Bacterial; D003227:Conjugation, Genetic; D003433:Crosses, Genetic; D004261:DNA Replication; D004269:DNA, Bacterial / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005111:Extrachromosomal Inheritance; D007785:Lactose / Q000378:metabolism; D009154:Mutation; D011995:Recombination, Genetic
chemical_list: D004269:DNA, Bacterial; D007785:Lactose