title: Single-strand breaks in deoxyribonucleic acid and viability loss during deoxyribonucleic acid synthesis inhibition in Escherichia coli.
abstract: The effects of deoxyribonucleic acid (DNA) synthesis inhibition brought about in four different ways-thymidine starvation, nalidixic acid, hydroxyurea, and dnaB mutation-were examined in isogenic strains of Escherichia coli K-12. Three parameters were examined to determine whether there are strict correlations among them: (i) the extent of DNA synthesis inhibition; (ii) cell survival; and (iii) the rate of breakage of DNA molecules. There was no significant correlation between the extent of DNA synthesis inhibition and the rate of viability loss caused by the four DNA synthesis inhibitors, nor was there a strict correlation between the rate of occurrence of single-strand breaks in DNA and loss of viability. During treatment with hydroxyurea (0.1 M), no viability loss was observed and little, if any, single-strand breakage of DNA occurred. Both thymidine starvation and nalidixic-acid (20 mug/ml) treatment resulted in viability loss and breakage of DNA. For these latter two inhibitors, the two events appeared to be associated because greater rates of both viability loss and DNA breakage were observed for nalidixic acid compared with thymidine starvation. However, viability loss need not be associated with extensive breakage of DNA as demonstrated with a temperature-sensitive DNA synthesis mutant; at 39 C, viability loss occurred at a high rate without significant DNA breakage. With the other agents, the amount of DNA breakage accumulated when a cell population has sustained an average of one lethal hit was estimated to be about 30 single-strand breaks per genome. Differences in chromosomal and episomal breakage rates were observed.
mesh_terms: D000225:Adenine / Q000378:metabolism; D002250:Carbon Radioisotopes; D002470:Cell Survival; D002876:Chromosomes, Bacterial / Q000032:analysis; D004269:DNA, Bacterial / Q000032:analysis / Q000096:biosynthesis*; D004277:DNA, Single-Stranded / Q000032:analysis; D004926:Escherichia coli / Q000032:analysis / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D006918:Hydroxyurea / Q000494:pharmacology; D008970:Molecular Weight; D009154:Mutation; D009268:Nalidixic Acid / Q000494:pharmacology; D009691:Nucleic Acid Denaturation; D013696:Temperature; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
chemical_list: D002250:Carbon Radioisotopes; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D014316:Tritium; D009268:Nalidixic Acid; D000225:Adenine; D013936:Thymidine; D006918:Hydroxyurea