Close linkage of the genes serC (for phosphohydroxy pyruvate transaminase) and serS (for seryl-transfer ribonucleic acid synthetase) in Escherichia coli K-12.
Escherichia coli strain K28, isolated after nitrosoguanidine mutagenesis, was found to be auxotrophic for serine. It was also temperature sensitive for growth as a result of producing an altered seryl-transfer ribonucleic acid (tRNA) synthetase (EC 6.1.1.11, l-serine: tRNA ligase [AMP]). The auxotrophy was caused by a mutation in the structural gene for phosphohydroxy-pyruvate transaminase (serC), which was distinct from, but closely linked to, the structural gene for seryl-tRNA synthetase (serS). We conclude that the relevant genes are in the order gal-serS-serC-aroA.
D000604:Amino Acyl-tRNA Synthetases / Q000096:biosynthesis* / Q000378:metabolism; D002247:Carbon Isotopes; D002474:Cell-Free System; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D005796:Genes; D008040:Genetic Linkage; D009153:Mutagens; D009154:Mutation; D009604:Nitrosoguanidines; D011773:Pyruvates; D011995:Recombination, Genetic; D012694:Serine / Q000378:metabolism; D013696:Temperature; D000637:Transaminases / Q000096:biosynthesis* / Q000378:metabolism; D014161:Transduction, Genetic
D002247:Carbon Isotopes; D009153:Mutagens; D009604:Nitrosoguanidines; D011773:Pyruvates; D012694:Serine; D000637:Transaminases; D000604:Amino Acyl-tRNA Synthetases