title: Relationship between sporulation-specific 20S ribonucleic acid and ribosomal ribonucleic acid processing in Saccharomyces cerevisiae.
abstract: The nature and properties of the 20S ribonucleic acid which accumulates only during the sporulation of Saccharomyces cerevisiae were examined. The 20S ribonucleic acid (RNA) has a base composition considerably different from ribosomal RNA species and is virtually unmethylated. The 20S RNA did, however, exhibit approximately 70% homology with 18S RNA by RNA-deoxyribonucleic acid filter hybridization competitions. The 20S RNA showed a hybridization saturation plateau level 30 to 40% higher than 18S, consistent with measurements of the size difference in polyacrylamide gels. Pulse-chase experiments in the presence and absence of cycloheximide indicate that the 20S RNA has a presumptive relationship to the 20S ribosomal RNA precursor normally observed only in short pulse-labeling in vegetative cells.
mesh_terms: D000085:Acetates; D000225:Adenine / Q000378:metabolism; D002247:Carbon Isotopes; D002854:Chromatography, Paper; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology; D004590:Electrophoresis, Paper; D004591:Electrophoresis, Polyacrylamide Gel; D008715:Methionine / Q000378:metabolism; D008745:Methylation; D009693:Nucleic Acid Hybridization; D010759:Phosphorus Isotopes; D011188:Potassium; D012313:RNA / Q000032:analysis / Q000096:biosynthesis; D012335:RNA, Ribosomal / Q000032:analysis / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D013170:Spores / Q000378:metabolism*; D013172:Spores, Fungal / Q000254:growth & development / Q000378:metabolism; D014316:Tritium
chemical_list: D000085:Acetates; D002247:Carbon Isotopes; D003470:Culture Media; D010759:Phosphorus Isotopes; D012335:RNA, Ribosomal; D014316:Tritium; D012313:RNA; D003513:Cycloheximide; D008715:Methionine; D000225:Adenine; D011188:Potassium