Location of the origin of transfer of the sex factor F.
The location of the origin of transfer on the map of Flac has been determined by two different techniques. The first showed that no transfer cistrons were transferred early by a transposition Hfr strain with a known point of chromosomal integration into Flac, and the second used a series of transfer-deficient Hfr deletion strains to map a locus required in cis for chromosome transfer by an incoming Flac factor. Both techniques gave results consistent with a location for the origin of transfer between traJ and the phi(R) (11) locus.
D000483:Alleles; D001483:Base Sequence; D002876:Chromosomes, Bacterial; D003227:Conjugation, Genetic; D003433:Crosses, Genetic; D004261:DNA Replication; D004269:DNA, Bacterial / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005111:Extrachromosomal Inheritance; D007785:Lactose / Q000378:metabolism; D009154:Mutation; D011995:Recombination, Genetic
D004269:DNA, Bacterial; D007785:Lactose