Persistent infection of cells in culture by measles virus. 3. Comparison of virus-specific RNA synthesized in primary persistent infection in HeLa cells.
The pattern of actinomycin D-resistant RNA synthesis was examined during primary infection of HeLa cells by virulent Edmonston measles virus and in two HeLa clones persistently infected by the same strain of virus. One of these clones, K11, produces infectious virus of low virulence for HeLa cells, and the other, K11A-HG-1, has thus far failed to yield infectious virus. The patterns of virus-specific RNA synthesized in these three types of infection are qualitatively similar to each other and to the patterns of virus-specific RNA synthesis in other paramyxovirus infections. There were, however, quantitative differences. In addition, virions of the virulent Edmonston strain of measles virus were found to contain high-molecular-weight RNA with a sedimentation constant identical to that of Newcastle disease virus.
D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D002999:Clone Cells; D003588:Cytopathogenic Effect, Viral; D003609:Dactinomycin / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D014644:Genetic Variation; D006367:HeLa Cells / Q000382:microbiology; D006801:Humans; D008459:Measles virus / Q000032:analysis / Q000254:growth & development* / Q000378:metabolism / Q000472:pathogenicity; D008970:Molecular Weight; D009522:Newcastle disease virus / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D014774:Virulence; D014779:Virus Replication
D002247:Carbon Isotopes; D012367:RNA, Viral; D014316:Tritium; D003609:Dactinomycin; D014529:Uridine