Interaction of eukaryote elongation factor EF 1 with guanosine nucleotides and aminoacyl-tRNA.
Evidence for two species of elongation factor 1 (EF 1(A) and EF 1(B)) from calf brain has been obtained by molecular sieve chromatography on Sephadex G-150. A high molecular weight form, EF 1(A), interacts with GTP to form an EF 1(A)-GTP complex. GDP also reacts with EF 1, but unlike the reaction with GTP, an EF 1(B)-GDP complex is formed that contains a lower molecular weight and labile species of EF 1. The results also indicate that EF 1(A)-GTP reacts with aminoacyl-tRNA to form an aminoacyl-tRNA-EF 1(B)-GTP complex. These results are discussed with regard to the role of EF 1 in aminoacyl-tRNA binding to ribosomes.
D000818:Animals; D001923:Brain Chemistry; D002247:Carbon Isotopes; D002417:Cattle; D002850:Chromatography, Gel; D004926:Escherichia coli / Q000032:analysis; D006150:Guanine Nucleotides / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism; D008956:Models, Chemical; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010441:Peptide Chain Elongation, Translational; D010649:Phenylalanine / Q000378:metabolism; D010759:Phosphorus Isotopes; D011485:Protein Binding; D012343:RNA, Transfer / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D014166:Transferases; D014316:Tritium
D002247:Carbon Isotopes; D006150:Guanine Nucleotides; D009419:Nerve Tissue Proteins; D010759:Phosphorus Isotopes; D014316:Tritium; D010649:Phenylalanine; D006160:Guanosine Triphosphate; D012343:RNA, Transfer; D014166:Transferases