Sedimentation properties of yeast chromosomal DNA.
Sedimentation analysis of nuclear DNA released from spheroplasts of the yeast Saccharomyces cerevisiae indicates that it has a number average molecular weight of 6.2 x 10(8). The chromosomal DNA molecules range in size from as small as 5 x 10(7) daltons to as large as 1.4 x 10(9) daltons. Based on these values and estimates of the total DNA content of the yeast nucleus, it is proposed that each yeast chromosome contains a single DNA duplex.
D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D002875:Chromosomes / Q000032:analysis*; D003090:Coliphages / Q000032:analysis; D004247:DNA / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis; D008970:Molecular Weight; D012440:Saccharomyces / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000032:analysis; D014316:Tritium; D014498:Uracil
D002247:Carbon Isotopes; D004279:DNA, Viral; D014316:Tritium; D014498:Uracil; D004247:DNA