title: Suppression of lex mutations affecting deoxyribonucleic acid repair in Escherichia coli K-12 by closely linked thermosensitive mutations.
abstract: A major class of ultraviolet (UV)-resistant derivatives of lex(-) strains of Escherichia coli K-12 grows normally at 30 C but at 42.5 C fails to produce colonies on complete or minimal agar. At 42.5 C these thermosensitive strains form filaments without septa, due to an apparent defect in cell division. Deoxyribonucleic acid degradation in UV-irradiated cultures of the thermosensitive strains is slow, in contrast to the rapid degradation in UV-irradiated cultures of the parental lex(-) strains. The thermosensitive mutations (tsl) are tightly linked (less than 0.04 min on the E. coli K-12 linkage map) to the site of the lex mutation in the parental strain and could lie within the same gene. The tsl(+)/tsl(-) heterozygotes grow at 42.5 C and are UV resistant when grown at 30 or 42.5 C. The tsl mutations are, therefore, recessive in contrast to lex mutations, which are dominant. It appears likely that the tsl mutations alter the diffusible product that gives rise to the Lex(-) mutant phenotype. This product appears to be necessary for deoxyribonucleic acid repair and cell division.
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis; D002455:Cell Division; D002874:Chromosome Mapping; D003470:Culture Media; D004260:DNA Repair; D004269:DNA, Bacterial / Q000378:metabolism*; D004171:Diploidy; D004926:Escherichia coli / Q000378:metabolism* / Q000528:radiation effects; D005796:Genes; D005808:Genes, Recessive; D008040:Genetic Linkage; D006579:Heterozygote; D006358:Hot Temperature; D007700:Kinetics; D009154:Mutation; D010641:Phenotype; D011831:Radiation Genetics; D014161:Transduction, Genetic; D014466:Ultraviolet Rays
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D004269:DNA, Bacterial