Protein synthesis and deoxyribonucleic acid-membrane attachment during thymineless death in Escherichia coli.
The proteins synthesized during thymineless death in Escherichia coli B and B/r were analyzed by polyacrylamide gel elctrophoresis. It was found that the amount of a protein of molecular weight 80,000 to 88,000 is greatly increased during thymineless death compared to the amounts of other cell proteins. A technique for the isolation of cell membrane-deoxyribonucleic acid (DNA)-nascent ribonucleic acid (RNA) complex on detergent crystals was used to determine whether DNA might be detached from the cell membrane as a result of thymineless death. It was found that under no conditions of thymineless death or immunity to thymineless death was there any change in the attachment of DNA or pulse-labeled RNA to cell membrane.
D001426:Bacterial Proteins / Q000032:analysis / Q000096:biosynthesis*; D001665:Binding Sites; D002247:Carbon Isotopes; D002462:Cell Membrane / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000032:analysis / Q000378:metabolism* / Q000528:radiation effects; D007930:Leucine / Q000378:metabolism; D008274:Magnesium; D008970:Molecular Weight; D009113:Muramidase; D009154:Mutation; D009829:Oleic Acids / Q000378:metabolism; D012329:RNA, Bacterial / Q000378:metabolism; D011830:Radiation Effects; D013501:Surface-Active Agents; D013936:Thymidine / Q000378:metabolism; D013941:Thymine / Q000378:metabolism*; D014316:Tritium; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D002247:Carbon Isotopes; D004269:DNA, Bacterial; D009829:Oleic Acids; D012329:RNA, Bacterial; D013501:Surface-Active Agents; D014316:Tritium; D009113:Muramidase; D007930:Leucine; D008274:Magnesium; D013941:Thymine; D013936:Thymidine