Bile acids. XXXVII. Identification of the 3 beta isomers of allocholic and allochenodeoxycholic acids as metabolites of 5 -cholestanol in the rat.
Bile was collected for 18-24 days from adult male rats with cannulated bile ducts that had received intraperitoneally 0.8 mg of 5alpha-[4-(14)C, 3alpha-(3)H]cholestan-3beta-ol. Bile from the first 2 days containing 14.2% of the administered (14)C and 3.3% of the (3)H was hydrolyzed, and the bile acids were separated by acetic acid partition chromatography. The previously unidentified metabolite more polar than cholic and allocholic acids was identified by isotopic dilution as 3beta,7alpha,12alpha-trihydroxy-5alpha-cholanic acid and represented 3% of the biliary (14)C and 15% of the (3)H. Similarly, 3beta,7alpha-dihydroxy-5alpha-cholanic acid was identified in fractions more polar than allochenodeoxycholic acid and represented 0.6% of the biliary (14)C and 8% of the (3)H. More polar fractions contained 4% of the (14)C and 31% of the (3)H in unidentified metabolites.
D000818:Animals; D001646:Bile / Q000378:metabolism*; D001647:Bile Acids and Salts / Q000096:biosynthesis* / Q000378:metabolism; D002247:Carbon Isotopes; D002635:Chenodeoxycholic Acid / Q000096:biosynthesis; D004083:Cholestanol / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism*; D002793:Cholic Acids / Q000096:biosynthesis; D002845:Chromatography; D007536:Isomerism; D008297:Male; D051381:Rats; D014316:Tritium
D001647:Bile Acids and Salts; D002247:Carbon Isotopes; D002793:Cholic Acids; D002635:Chenodeoxycholic Acid; D014316:Tritium; D004083:Cholestanol; D002784:Cholesterol