title: Regulation of the expression of the N gene of bacteriophage lambda.
abstract: A quantitative assay for the N protein of bacteriophage lambda has been used to study the in vivo regulation of N gene expression. The assay makes use of the observation that in a cell-free protein-synthesizing system from Escherichia coli programmed with lambdaN(-) DNA the lambda endolysin is made only if N protein is added to the reaction. The rate of synthesis of N protein in vivo is negatively controlled by the products of the CI and tof genes of the phage. Furthermore, N protein activity is extremely unstable in vivo. During normal cell growth at 35 degrees , the half-life of N protein is about 2 min.
mesh_terms: D002474:Cell-Free System; D002874:Chromosome Mapping; D003090:Coliphages / Q000201:enzymology / Q000378:metabolism*; D010450:Endopeptidases / Q000032:analysis / Q000096:biosynthesis; D004926:Escherichia coli; D005796:Genes; D005809:Genes, Regulator; D007700:Kinetics; D008242:Lysogeny; D013696:Temperature; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: D014764:Viral Proteins; D010450:Endopeptidases