The use of deoxyfluoro-D-glucopyranoses and related compounds in a study of yeast hexokinase specificity.
1. 2-Deoxy-2-fluoro-d-glucose, 2-deoxy-2-fluoro-d-mannose and 2-deoxy-2,2-difluoro-d-arabino-hexose are good substrates for yeast hexokinase. 2. 3-Deoxy-3-fluoro-d-glucose and 4-deoxy-4-fluoro-d-glucose are poor substrates and have very similar K(m) values (8x10(-2)m). 3. Neither alpha- nor beta-d-glucopyranosyl fluoride is a substrate or inhibitor. 4. Studies with 2-chloro-2-deoxy- and 2-O-methyl derivatives of d-glucose and d-mannose have revealed that little chemical modification is possible at position 2 without substantial loss in substrate binding. 5. The variation in the value of K(m) for the d-hexose derivatives was associated with a corresponding change in the value of K(m) for MgATP(2-) showing that the binding of MgATP(2-) is modified by the binding of the sugar.
D000255:Adenosine Triphosphate; D002712:Chlorides; D005459:Fluorides; D005947:Glucose; D006593:Hexokinase; D006601:Hexoses; D007700:Kinetics; D008274:Magnesium; D008358:Mannose; D012441:Saccharomyces cerevisiae / Q000201:enzymology
D002712:Chlorides; D006601:Hexoses; D000255:Adenosine Triphosphate; D006593:Hexokinase; D008274:Magnesium; D005947:Glucose; D008358:Mannose; D005459:Fluorides