Ribonucleotides covalently linked to deoxyribonucleic acid in T4 bacteriophage.
Bacteriophage T4 was grown in the presence of labeled uridine. The deoxyribonucleic acid (DNA) of the phage was shown to contain covalently attached ribonucleotides. The label appears not to be internal in the DNA strands. Presumably, it is at the ends of the DNA strands and this may be related to DNA initiation.
D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D002855:Chromatography, Thin Layer; D003090:Coliphages / Q000032:analysis* / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D004261:DNA Replication; D004279:DNA, Viral / Q000032:analysis* / Q000096:biosynthesis; D003851:Deoxyribonucleases; D004926:Escherichia coli; D006358:Hot Temperature; D009691:Nucleic Acid Denaturation; D011402:Pronase; D012367:RNA, Viral / Q000032:analysis; D012260:Ribonucleases; D012265:Ribonucleotides / Q000032:analysis*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D002247:Carbon Isotopes; D004279:DNA, Viral; D012367:RNA, Viral; D012265:Ribonucleotides; D014316:Tritium; D003851:Deoxyribonucleases; D012260:Ribonucleases; D011402:Pronase; D013936:Thymidine; D014529:Uridine