Surface antigens of immunocompetent cells. 3. In vitro studies of the role of B and T cells in immunological memory.
The effect of preincubation with anti-theta or anti-mouse immunoglobulin (Ig) and complement (C') on immune responsiveness of spleen cells from BALB/c mice immunized with sheep erythrocytes (SE) was investigated. Both treatments greatly depressed the remaining ability to produce a secondary response to SE in vitro. Normal BALB/c spleen cells were far less effective in reconstituting the responses of such depleted cell populations than were much smaller numbers of untreated immune spleen cells. Thymus-derived cell (T cell) memory appeared early after immunization and showed specificity for the immunizing antigens. Recombination of anti-Ig-treated with anti-theta-treated immune spleen cells resulted in virtually complete reconstitution of responsiveness. The presence of immunological memory in T cells and the nature of their surface receptors are discussed.
D000818:Animals; D000917:Antibody Formation; D000921:Antibody-Producing Cells; D000941:Antigens; D001853:Bone Marrow / Q000276:immunology*; D001854:Bone Marrow Cells; D002462:Cell Membrane / Q000276:immunology*; D002478:Cells, Cultured; D003165:Complement System Proteins; D004912:Erythrocytes / Q000276:immunology; D006462:Hemolytic Plaque Technique; D007106:Immune Sera; D007112:Immunity, Maternally-Acquired; D007074:Immunoglobulin G; D007156:Immunologic Memory; D007165:Immunosuppression Therapy; D007942:Leukemia, Experimental / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D012756:Sheep / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology*
D000941:Antigens; D007106:Immune Sera; D007074:Immunoglobulin G; D003165:Complement System Proteins