Initiation factor 2-dependent ribosomal binding of N-formylmethionyl-transfer RNA without added guanosine triphosphate.
Evidence is presented that suggests that GTP (or 5'-guanylylmethylene diphosphonate) is not essential for either formation of an AUG-directed, initiation factor IF-2-dependent initiation complex on the 30S subunit or for positioning of fMet-tRNA on the peptidyl site. However, recycling of limiting amounts of IF-2 requires both GTP and the 50S subunit. The formation of the complex IF-2-30S as an intermediate in polypeptidechain initiation is suggested.
D001665:Binding Sites; D002247:Carbon Isotopes; D004926:Escherichia coli / Q000378:metabolism; D005561:Formates / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000494:pharmacology*; D008715:Methionine / Q000378:metabolism; D009994:Osmolar Concentration; D010442:Peptide Chain Initiation, Translational / Q000187:drug effects; D010448:Peptide Initiation Factors / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D011691:Puromycin / Q000494:pharmacology; D012329:RNA, Bacterial / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D013268:Stimulation, Chemical
D002247:Carbon Isotopes; D005561:Formates; D010448:Peptide Initiation Factors; D012329:RNA, Bacterial; D011691:Puromycin; D006160:Guanosine Triphosphate; D012343:RNA, Transfer; D008715:Methionine