title: Relation between protein synthesis and phospholipid synthesis and turnover in Escherichia coli.
abstract: Turnover of phospholipids occurred continuously in a fatty acid auxotroph of Escherichia coli, but in the wild-type parent strain significant turnover occurred only under conditions where protein synthesis was inhibited but lipid synthesis continued. Both strains gave a stringent response of ribonucleic acid accumulation to amino acid starvation, but only in the wild type was lipid synthesis also inhibited. A revertant strain of the auxotroph resembled the wild type in this respect. The phospholipid that accumulates in the culture medium as a result of the lipid turnover appears to be part of a loosely bound low-density complex arising from the cell envelope.
mesh_terms: D000085:Acetates / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis*; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D002701:Chloramphenicol / Q000494:pharmacology; D002751:Chlortetracycline / Q000494:pharmacology; D002850:Chromatography, Gel; D004926:Escherichia coli / Q000032:analysis / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005809:Genes, Regulator; D007930:Leucine / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis; D009154:Mutation; D009829:Oleic Acids / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D012329:RNA, Bacterial / Q000096:biosynthesis; D014316:Tritium; D014461:Ultracentrifugation; D014498:Uracil / Q000378:metabolism
chemical_list: D000085:Acetates; D001426:Bacterial Proteins; D002247:Carbon Isotopes; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D008055:Lipids; D009829:Oleic Acids; D010743:Phospholipids; D012329:RNA, Bacterial; D014316:Tritium; D014498:Uracil; D002701:Chloramphenicol; D007930:Leucine; D002751:Chlortetracycline