Isolation of covalently closed circular deoxyribonucleic acid from bacteria which produce exocellular nuclease.
Reproducible yields of covalently closed circular (plasmid) deoxyribonucleic acid were obtained from mutants defective for extracellular nuclease but not from the corresponding wild-type strain of Serratia marcescens
D002247:Carbon Isotopes; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D004261:DNA Replication; D004269:DNA, Bacterial / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification*; D004270:DNA, Circular / Q000302:isolation & purification; D004720:Endonucleases / Q000096:biosynthesis* / Q000378:metabolism; D005111:Extrachromosomal Inheritance; D009154:Mutation; D012706:Serratia marcescens / Q000032:analysis* / Q000201:enzymology / Q000378:metabolism; D014316:Tritium
D002247:Carbon Isotopes; D004269:DNA, Bacterial; D004270:DNA, Circular; D014316:Tritium; D004720:Endonucleases