Relationship between methionyl transfer ribonucleic acid cellular content and synthesis of methionine enzymes in Saccharomyces cerevisiae.
Derepression of some methionine biosynthetic enzymes (methionine group I enzymes) obtained in methionine limitation has been found to be accompanied by a significant lack of in vivo charging of bulk methionine transfer ribonucleic acid (tRNA(Met)) and in addition by a decreased rate of synthesis of all tRNAs. Under the same conditions, methionyl-tRNA synthetase (MTS) was derepressed rather than repressed. These results are in agreement with those previously published based on studies of a mutant with an impaired MTS (5) and reinforce the idea that the rate of synthesis of methionine group I enzymes can be related to the total content of methionyl (Met)-tRNA (Met) per cell. They also render unlikely that MTS could be a constituent of the regulatory signal.
D000255:Adenosine Triphosphate; D000604:Amino Acyl-tRNA Synthetases / Q000096:biosynthesis* / Q000378:metabolism; D002247:Carbon Isotopes; D002474:Cell-Free System; D006710:Homocysteine; D007532:Isoleucine / Q000378:metabolism; D008025:Ligases / Q000096:biosynthesis* / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D009154:Mutation; D009713:Nucleotidyltransferases / Q000096:biosynthesis* / Q000378:metabolism; D012343:RNA, Transfer / Q000032:analysis / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000032:analysis / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D013237:Stereoisomerism; D014316:Tritium
D002247:Carbon Isotopes; D007532:Isoleucine; D006710:Homocysteine; D014316:Tritium; D000255:Adenosine Triphosphate; D012343:RNA, Transfer; D008715:Methionine; D009713:Nucleotidyltransferases; D008025:Ligases; D000604:Amino Acyl-tRNA Synthetases