A new element in the control of DNA initiation in Escherichia coli.
When a thermosensitive mutant of E. coli affected in DNA initiation is heated to 41 degrees , a protein needed for DNA initiation is irreversibly denatured. When these bacteria are incubated at 30 degrees in the presence of several concentrations of chloramphenicol, DNA synthesis is greatly stimulated at a chloramphenicol, DNA synthesis is greatly stimulated at a chloramphenicol concentration of 2.5 mug/ml, and then sharply decreases. This stimulation of DNA synthesis appears to be caused by an increased frequency of initiation. The existence of an "anti-initiator" protein controlling the frequency of chromosomal initiation is proposed.
D001426:Bacterial Proteins / Q000096:biosynthesis; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D002701:Chloramphenicol / Q000494:pharmacology; D002876:Chromosomes, Bacterial / Q000187:drug effects / Q000378:metabolism; D003080:Cold Temperature; D003470:Culture Media; D004261:DNA Replication / Q000187:drug effects; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D005815:Genetic Code; D006358:Hot Temperature; D007930:Leucine / Q000378:metabolism; D009154:Mutation; D010442:Peptide Chain Initiation, Translational / Q000187:drug effects*; D010448:Peptide Initiation Factors / Q000494:pharmacology; D011489:Protein Denaturation / Q000187:drug effects; D013941:Thymine / Q000378:metabolism
D001426:Bacterial Proteins; D002247:Carbon Isotopes; D003470:Culture Media; D004269:DNA, Bacterial; D010448:Peptide Initiation Factors; D002701:Chloramphenicol; D007930:Leucine; D013941:Thymine