Determination of the length of the V colicinogenic factor and a derivative fertility factor.
Plasmid deoxyribonucleic acid from Escherichia coli harboring either the V colicinogenic factor (Col V) or the derivative fertility factor (Fv), (a non-colicin-producing fertility factor derived from Col V) were separated by cesium chloride-ethidium bromide centrifugation and examined by electron microscopy. Results indicate a deletion of 11 mum of deoxyribonucleic acid in the transition from Col V to Fv.
D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D002876:Chromosomes, Bacterial; D003087:Colicins / Q000096:biosynthesis*; D004269:DNA, Bacterial / Q000032:analysis* / Q000302:isolation & purification; D004926:Escherichia coli / Q000032:analysis* / Q000378:metabolism; D004996:Ethidium; D005111:Extrachromosomal Inheritance; D008854:Microscopy, Electron; D008970:Molecular Weight; D013941:Thymine; D014316:Tritium
D002712:Chlorides; D003087:Colicins; D004269:DNA, Bacterial; D014316:Tritium; D002586:Cesium; D004996:Ethidium; D013941:Thymine