Polyamine metabolism in potassium-deficient bacteria.
The metabolism of polyamines was studied in K(+)-dependent strains of Escherichia coli. When these stringent organisms were in a medium containing Na(+) instead of K(+), protein synthesis was arrested, but synthesis of ribonucleic acid continued as it would in a relaxed organism. The Na(+) medium inhibited synthesis of spermidine and S-adenosylmethionine. However, the synthesis of putrescine was accelerated at least five- to eightfold. Exogenous ornithine doubled even this rate of putrescine synthesis but did not increase the low level of putrescine synthesis in the K(+) medium. In K(+) or Na(+) media, with or without 0.3 mm arginine, putrescine was derived almost entirely from ornithine via ornithine decarboxylase. Addition of spermidine (5 mm) to a Na(+) culture markedly inhibited putrescine synthesis. The ornithine decarboxylase of an extract of a K(-)-dependent strain prepared at low ionic strength was separated from ribosomes, deoxyribonucleic acid, and associated polyamines by centrifugation, and from many ions by ultrafiltration and fractionation on Sephadex G-100. Addition of Na(+) and K(+) salts to 200 mm was markedly inhibitory. The combined reductions both in synthesis of the inhibitor spermidine and in intracellular ionic strength may explain the in vivo activation of this enzyme.
D001120:Arginine / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis; D002247:Carbon Isotopes; D002262:Carboxy-Lyases / Q000378:metabolism; D002474:Cell-Free System; D003470:Culture Media; D004926:Escherichia coli / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D009154:Mutation; D009952:Ornithine / Q000378:metabolism; D011188:Potassium / Q000378:metabolism*; D011700:Putrescine / Q000096:biosynthesis*; D012329:RNA, Bacterial / Q000096:biosynthesis; D012436:S-Adenosylmethionine / Q000096:biosynthesis; D012964:Sodium / Q000378:metabolism; D013095:Spermidine / Q000096:biosynthesis* / Q000378:metabolism; D014508:Urea / Q000096:biosynthesis
D001426:Bacterial Proteins; D002247:Carbon Isotopes; D003470:Culture Media; D012329:RNA, Bacterial; D012436:S-Adenosylmethionine; D014508:Urea; D001120:Arginine; D012964:Sodium; D008715:Methionine; D009952:Ornithine; D002262:Carboxy-Lyases; D011188:Potassium; D013095:Spermidine; D011700:Putrescine