title: Preparation of gram quantities of a purified R-factor-mediated penicillinase from Escherichia coli strain W3310.
abstract: Purified penicillinase, in gram quantities, has been prepared from Escherichia coli strain W3310 by using methods developed to handle large amounts of material. The final product had a specific enzyme activity of 3.08 units/mug of protein, which was over twice as high as that reported previously (Datta & Richmond, 1966). The purified enzyme was similar to that from E. coli strain TEM, but different in molecular weight and some other respects. The differences observed may be a result of the greater purity obtained.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000645:Ammonium Sulfate; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003956:Dialysis; D004352:Drug Resistance, Microbial; D004591:Electrophoresis, Polyacrylamide Gel; D004592:Electrophoresis, Starch Gel; D004926:Escherichia coli / Q000201:enzymology*; D008722:Methods; D008970:Molecular Weight; D010405:Penicillinase / Q000302:isolation & purification*
chemical_list: D000596:Amino Acids; D010405:Penicillinase; D000645:Ammonium Sulfate