Fermentation process for double-stranded ribonucleic acid, an interferon inducer.
Double-stranded ribonucleic acid (ds-RNA) isolated from Escherichia coli infected with bacteriophage MS2 is a potent interferon inducer. High levels of ds-RNA are formed in nonpermissive cells infected with MU9, an amber coat protein mutant of MS2. This mutant has been used to develop a process for large-scale ds-RNA production. Preparation of quantities of MU9 lysate sufficient for ds-RNA production in fermentors is described. Over 300 mug of ds-RNA/ml can be accumulated after MU9 infection of cultures grown to high density in corn steep liquor medium. This is approximately 300 times the amount of ds-RNA made by MS2 infection of cells grown in tryptone medium. Maximum ds-RNA formation requires only 3 hr. The ds-RNA is stable and remains inside nonaerated cells for at least 17 hr.
D000388:Air; D003090:Coliphages / Q000254:growth & development / Q000378:metabolism; D003470:Culture Media; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism; D005285:Fermentation; D007369:Interferon Inducers; D008242:Lysogeny; D008722:Methods; D009154:Mutation; D010461:Peptones; D012367:RNA, Viral / Q000096:biosynthesis*; D013696:Temperature; D014357:Trypsin; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication; D003313:Zea mays
D003470:Culture Media; D007369:Interferon Inducers; D010461:Peptones; D012367:RNA, Viral; D014764:Viral Proteins; D014357:Trypsin