title: Messenger ribonucleic acid synthesis and degradation in Escherichia coli during inhibition of translation.
abstract: Various aspects of the coupling between the movement of ribosomes along messenger ribonucleic acids (mRNA) and the synthesis and degradation of mRNA have been investigated. Decreasing the rate of movement of ribosomes along an mRNA does not affect the rate of movement of some, and possibly most, of the RNA polymerases transcribing the gene coding for that mRNA. Inhibiting translation with antibiotics such as chloramphenicol, tetracycline, or fusidic acid protects extant mRNA from degradation, presumably by immobilizing ribosomes, whereas puromycin exposes mRNA to more rapid degradation than normal. The promoter distal (3') portion of mRNA, synthesized after ribosomes have been immobilized by chloramphenicol on the promoter proximal (5') portion of the mRNA, is subsequently degraded.
mesh_terms: D002250:Carbon Radioisotopes; D003470:Culture Media; D004926:Escherichia coli / Q000378:metabolism*; D005696:Galactosidases / Q000378:metabolism; D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism*; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D013997:Time Factors; D014316:Tritium
chemical_list: D002250:Carbon Radioisotopes; D003470:Culture Media; D012329:RNA, Bacterial; D012333:RNA, Messenger; D014316:Tritium; D005696:Galactosidases