General method for the isolation of plasmid deoxyribonucleic acid.
Plasmid deoxyribonucleic acid (DNA) ranging from 5 x 10(6) to 65 x 10(6) daltons may be isolated from chromosomal DNA by the preferential precipitation of the higher-molecular-weight chromosomal DNA in the presence of sodium lauryl sulfate and a high concentration of NaCl.
D002498:Centrifugation; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004926:Escherichia coli; D005111:Extrachromosomal Inheritance; D008722:Methods; D012965:Sodium Chloride; D014316:Tritium
D004269:DNA, Bacterial; D014316:Tritium; D012965:Sodium Chloride