Isolation of temperature-sensitive membrane mutants of Escherichia coli K-12.
Mutants with impaired biosynthesis of unsaturated fatty acids or altered metabolism of the phospholipids were isolated at a rather high frequency from a set of temperature-sensitive lysis mutants. It is suggested that preselection for the lysis phenotype makes it possible to isolate several kinds of mutants affected in the integrity of the cytoplasmic membrane.
D000085:Acetates / Q000378:metabolism; D002250:Carbon Radioisotopes; D002462:Cell Membrane / Q000502:physiology*; D002463:Cell Membrane Permeability; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D003470:Culture Media; D003593:Cytoplasm / Q000502:physiology; D004926:Escherichia coli / Q000302:isolation & purification*; D005231:Fatty Acids, Unsaturated; D007700:Kinetics; D050356:Lipid Metabolism; D009154:Mutation; D010641:Phenotype; D010710:Phosphates / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D013696:Temperature; D013997:Time Factors
D000085:Acetates; D002250:Carbon Radioisotopes; D003470:Culture Media; D005231:Fatty Acids, Unsaturated; D010710:Phosphates; D010743:Phospholipids; D010761:Phosphorus Radioisotopes