title: Effect of iron and salt on prodigiosin synthesis in Serratia marcescens.
abstract: Serratia marcescens wild-types ATCC 264 and Nima grew but did not synthesize prodigiosin in a glycerol-alanine medium containing 10 ng of Fe per ml. Wild-type 264 required the addition of 0.2 mug of Fe per ml for maximal growth and prodigiosin synthesis; Nima required 0.5 mug of Fe per ml. Three percent, but not 0.1%, sea salts inhibited prodigiosin synthesis in a complex medium containing up to 10 mug of Fe per ml. NaCl was the inhibitory sea salt component. The inhibition was not specific for NaCl; equimolar concentrations of Na(2)SO(4), KCl, and K(2)SO(4) also inhibited prodigiosin synthesis. Experiments with strains 264 and Nima and with mutant WF which cannot synthesize 4-methoxy-2-2'-bipyrrole-5-carboxyaldehyde (MBC), the bipyrrole moiety of prodigiosin, and with mutant 9-3-3 which cannot synthesize the monopyrrole moiety 2-methyl-3-amylpyrrole (MAP) showed that both MBC synthesis and the reaction condensing MAP and MBC to form prodigiosin were relatively more sensitive to NaCl inhibition than the MAP-synthesizing step. The capacity of whole cells to condense MAP and MBC was present, but inactive, in cells grown in NaCl; removal of the NaCl from non-proliferating salt-grown cells restored the activity. Other evidence suggests the existence of a common precursor to the MAP- and MBC-synthesizing pathways.
mesh_terms: D000447:Aldehydes / Q000096:biosynthesis; D003470:Culture Media; D007501:Iron / Q000494:pharmacology*; D009154:Mutation; D010858:Pigmentation / Q000187:drug effects; D011189:Potassium Chloride / Q000494:pharmacology; D011353:Prodigiosin / Q000096:biosynthesis*; D011758:Pyrroles / Q000096:biosynthesis; D012706:Serratia marcescens / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology*; D013054:Spectrophotometry, Atomic; D013431:Sulfates / Q000494:pharmacology
chemical_list: D000447:Aldehydes; D003470:Culture Media; D011758:Pyrroles; D013431:Sulfates; D012965:Sodium Chloride; D011189:Potassium Chloride; D007501:Iron; D011353:Prodigiosin