Messenger ribonucleic acid stability in relaxed and stringent Escherichia coli starved for methionine.
During starvation for each of four amino acids, relaxed and stringent strains of Escherichia coli showed exponential decay of pulse-labeled unstable messenger ribonucleic acid (mRNA), with RNA degraded more slowly in the relaxed strain. An additional unique difference was observed during starvation for methionine: the relaxed strain showed non-exponential decay of mRNA, with a survival curve similar to that of an aging process.
D002499:Centrifugation, Density Gradient; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D007532:Isoleucine / Q000378:metabolism; D008715:Methionine / Q000378:metabolism*; D009154:Mutation; D009693:Nucleic Acid Hybridization; D012329:RNA, Bacterial / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012293:Rifampin / Q000494:pharmacology; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D014633:Valine / Q000378:metabolism
D012329:RNA, Bacterial; D012333:RNA, Messenger; D007532:Isoleucine; D014316:Tritium; D008715:Methionine; D014633:Valine; D012293:Rifampin; D014529:Uridine