Translation of bacteriophage Q  RNA by cytoplasmic extracts of mammalian cells.
Cytoplasmic extracts from Krebs II mouse ascites cells and from L cells translate messenger RNA from coliphage Qbeta with fidelity to produce products that migrate on polyacrylamide gels with those products directed by Qbeta RNA in an Escherichia coli cell-free system. The mammalian cell extracts correctly initiate and terminate Qbeta coat protein synthesis, as shown by: (i) [(3)H]lysine-and [(3)H]arginine-labeled tryptic peptides derived from the coat-sized product resemble these from authentic Qbeta coat protein, (ii) Qbeta coat (which contains methionine only at the N-terminal end) can be radioactively labeled with methionine only if the methionine is formylated, and (iii) L cell extracts directed by Qbeta am(-)11 (an amber mutant in the coat protein) RNA make no completed coat-sized material, but do make a peptide the size of the authentic amber coat fragment.
D000818:Animals; D002247:Carbon Isotopes; D002287:Carcinoma, Krebs 2 / Q000378:metabolism*; D002474:Cell-Free System; D002478:Cells, Cultured; D003090:Coliphages / Q000032:analysis; D003593:Cytoplasm; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D005260:Female; D007700:Kinetics; D007739:L Cells / Q000378:metabolism*; D051379:Mice; D010455:Peptides / Q000032:analysis; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000302:isolation & purification / Q000378:metabolism*; D013460:Sulfur Isotopes; D014316:Tritium; D014357:Trypsin / Q000378:metabolism; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism
D002247:Carbon Isotopes; D010455:Peptides; D012333:RNA, Messenger; D012367:RNA, Viral; D013460:Sulfur Isotopes; D014764:Viral Proteins; D014316:Tritium; D014357:Trypsin