Stimulation of IgG antibody response in vitro by T cell-replacing factor.
A soluble factor (TRF) produced by mixtures of allogeneic mouse spleen, lymph node, and thymus cells functionally replaces T cells in a primary IgM antibody response to sheep blood cells in vitro. It is now shown that TRF can also reconstitute an IgG antibody response in T cell-deprived spleen cultures derived from preimmunized mice. The optimal time of addition and the amount of TRF required differ between primary and secondary in vitro systems.
D000818:Animals; D000917:Antibody Formation; D000941:Antigens; D004912:Erythrocytes / Q000276:immunology; D006462:Hemolytic Plaque Technique; D007106:Immune Sera; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis*; D051379:Mice; D012756:Sheep / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000941:Antigens; D007106:Immune Sera; D007074:Immunoglobulin G