Competitive inhibition and substrate activity of uridine diphosphate 6-deoxygalactose for Escherichia coli uridine diphosphate galactose 4-epimerase.
UDP-6-deoxygalactose inhibits the UDP-galactose 4-epimerase (EC 5.1.3.2) from Escherichia coli in a competitive manner with respect to the substrate UDP-galactose, giving K(i) 1.3x10(-3)m. As a substrate for the enzyme, it is transformed into UDP-6-deoxyglucose, although the reaction stops before equilibrium is attained. Possible causes of this behaviour are discussed.
D001667:Binding, Competitive; D002238:Carbohydrate Epimerases / Q000037:antagonists & inhibitors*; D002855:Chromatography, Thin Layer; D003903:Deuterium; D004926:Escherichia coli / Q000201:enzymology*; D005690:Galactose; D005947:Glucose; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D013997:Time Factors; D014539:Uridine Diphosphate Sugars
D014539:Uridine Diphosphate Sugars; D003903:Deuterium; D002238:Carbohydrate Epimerases; D005947:Glucose; D005690:Galactose