Improved hemagglutination test for identifying type A strains of Pasteurella multocida.
A simple, improved, indirect hemagglutination test is described for the recognition of Type A strains of Pasteurella multocida. It involves the treatment of mucoid cultures with testicular hyaluronidase. Hydrolysis of the capsular hyaluronic acid presumably releases the specific antigen for adsorption to erythrocytes.
D000942:Antigens, Bacterial / Q000032:analysis; D001431:Bacteriological Techniques; D004912:Erythrocytes / Q000276:immunology; D005069:Evaluation Studies as Topic; D006386:Hemagglutination Tests; D006358:Hot Temperature; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis; D006821:Hyaluronoglucosaminidase; D008297:Male; D010325:Pasteurella / Q000145:classification* / Q000378:metabolism; D012703:Serotyping; D013737:Testis / Q000201:enzymology
D000942:Antigens, Bacterial; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase