Polynucleotide ligase in bacteriophage T4D recombination.
Following infection of E. coli B with ligase-deficient rII bacteriophage T4D recombination between linked markers is increased 4.2 fold and heterozygote frequency increased 2.3 fold. In such infection recombination occurs at a rapid rate for an extended period. This is in contrast to the time course of recombination observed in wild-type, lysis-inhibited, or lysis-defective (gene t defective) infection. In all of these cases recombination under standard cross conditions occurs early in the vegetative cycle. The increased recombination in ligase-deficient rII infection is reduced in a bacterial strain which produces greater than normal levels of host ligase. These results indicate that ligase has a crucial role not only in the replication of DNA but also in recombination. The level of ligase may determine whether DNA replication occurs with or without concomitant recombination.
D001433:Bacteriolysis; D003090:Coliphages / Q000201:enzymology*; D004261:DNA Replication; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D005808:Genes, Recessive; D006579:Heterozygote; D007700:Kinetics; D009154:Mutation; D011118:Polynucleotide Ligases; D011995:Recombination, Genetic; D013696:Temperature; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D014316:Tritium; D011118:Polynucleotide Ligases; D013936:Thymidine