Effect of elevated temperatures on protein synthesis in Escherichia coli.
Upon a temperature shift from 30 C to 42 to 44 C, Escherichia coli experiences a transient slowdown in rate of optical density increase, uridine incorporation, and amino acid incorporation. The data show that the primary target for thermal inactivation is a molecule(s) required for the initiation of protein synthesis. The effect on ribonucleic acid synthesis is a secondary effect.
D001426:Bacterial Proteins / Q000096:biosynthesis*; D002247:Carbon Isotopes; D002474:Cell-Free System; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D006358:Hot Temperature; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D010649:Phenylalanine / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism; D011485:Protein Binding; D012329:RNA, Bacterial / Q000096:biosynthesis; D012967:Sodium Dodecyl Sulfate; D013347:Subcellular Fractions / Q000378:metabolism; D013460:Sulfur Isotopes; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D001426:Bacterial Proteins; D002247:Carbon Isotopes; D012329:RNA, Bacterial; D013460:Sulfur Isotopes; D014316:Tritium; D012967:Sodium Dodecyl Sulfate; D010649:Phenylalanine; D008715:Methionine; D012321:DNA-Directed RNA Polymerases; D007930:Leucine; D014529:Uridine