Genetic map of the lactose repressor gene (i) of Escherichia coli.
39 tonB i(-) deletions were used to map 57 independently isolated i gene mutants. Methods selective for i(+) recombinants from various types of i mutants are described. i(+) recombination frequencies of 6 x 10(-6) to 6 x 10(-7) can be detected in the different selective systems. Twenty i(s) mutations and 12 i(-d) mutations map in distinct but different regions of the gene. i(-) (sus) mutations are scattered over the gene.
D002869:Chromosome Aberrations; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D003470:Culture Media; D004926:Escherichia coli / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D005809:Genes, Regulator; D005816:Genetic Complementation Test; D006596:Hexosaminidases / Q000032:analysis; D007785:Lactose / Q000378:metabolism*; D008967:Molecular Biology; D009154:Mutation; D011995:Recombination, Genetic
D003470:Culture Media; D006596:Hexosaminidases; D007785:Lactose