Recognition of nonsense codons in mammalian cells.
The tritiated trinucleotide UGA was used in a binding assay to detect transfer RNAs that recognize this nonsense codon from calf-liver cells. Acylation of transfer RNA with labeled amino acids and determination of codon responses of aminoacyl-tRNAs demonstrate that a species of seryl-tRNA and a species of arginyl-tRNA recognize the codon UGA. Slight responses of cysteinyl-tRNA and of trytophanyl-tRNA to the codon UGA were also observed.
D000215:Acylation; D000227:Adenine Nucleotides / Q000378:metabolism; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D000818:Animals; D001120:Arginine / Q000378:metabolism; D002417:Cattle; D003545:Cysteine / Q000378:metabolism; D004926:Escherichia coli; D005815:Genetic Code; D006150:Guanine Nucleotides / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D009841:Oligonucleotides / Q000378:metabolism; D011485:Protein Binding; D012343:RNA, Transfer / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D012694:Serine / Q000378:metabolism; D013489:Suppression, Genetic; D014316:Tritium; D014364:Tryptophan / Q000378:metabolism; D014500:Uracil Nucleotides / Q000378:metabolism
D000227:Adenine Nucleotides; D006150:Guanine Nucleotides; D009841:Oligonucleotides; D014500:Uracil Nucleotides; D014316:Tritium; D012694:Serine; D014364:Tryptophan; D012343:RNA, Transfer; D001120:Arginine; D000604:Amino Acyl-tRNA Synthetases; D003545:Cysteine