Evidence for the existence of two arginyl-transfer ribonucleic acid synthetase activities in Escherichia coli.
Two arginyl-transfer ribonucleic acid (tRNA) synthetase (EC 6.1.1.13, arginine: ribonucleic acid ligase adenosine monophosphate) activities were found in extracts of Escherichia coli strains AB1132 and NP2. The two arginyl-tRNA synthetase activities in extracts of strain AB1132 were found to be separable by diethylaminoethyl-cellulose column chromatography, Sephadex column fractionation, and by sucrose density gradient centrifugation. In addition, in the standard assay using extracts of strain AB1132 there were two pH optima for arginyl-tRNA synthetase activity. Furthermore, when arginyl-tRNA synthetase of strain NP2 was fractionated by hydroxylapatite column chromatography, two activities were observed which were similar to those of strain AB1132.
D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification / Q000378:metabolism*; D001120:Arginine / Q000378:metabolism; D002247:Carbon Isotopes; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D002848:Chromatography, DEAE-Cellulose; D002854:Chromatography, Paper; D004926:Escherichia coli / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D006882:Hydroxyapatites; D013395:Sucrose
D002247:Carbon Isotopes; D006882:Hydroxyapatites; D013395:Sucrose; D001120:Arginine; D000604:Amino Acyl-tRNA Synthetases