title: Temperature-sensitive mutation in regulation of ribonucleic acid synthesis in Escherichia coli.
abstract: An Escherichia coli mutant dependent on exogenous transfer ribonucleic acid (RNA) for bulk RNA formation at 42 C has been isolated, starting from a parental strain permeable to RNA. In the absence of added transfer RNA at the high temperature, protein synthesis stopped, and the strain formed little if any ribosomal RNA.
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis; D002247:Carbon Isotopes; D002701:Chloramphenicol / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D005696:Galactosidases / Q000378:metabolism; D007553:Isotope Labeling; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D009154:Mutation; D009693:Nucleic Acid Hybridization; D012329:RNA, Bacterial / Q000096:biosynthesis*; D012335:RNA, Ribosomal / Q000096:biosynthesis; D012343:RNA, Transfer / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D013696:Temperature; D014316:Tritium; D014498:Uracil / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002247:Carbon Isotopes; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D014316:Tritium; D014498:Uracil; D002701:Chloramphenicol; D012343:RNA, Transfer; D005696:Galactosidases; D007930:Leucine; D014529:Uridine