title: Nitrogenase of Klebsiella pneumoniae: evidence for an adenosine triphosphate-induced association of the iron-sulphur protein.
abstract: The effect of various nucleotides on the Fe-containing component of nitrogenase of Klebsiella pneumoniae was investigated by ultracentrifugation and thiol-group reactivity towards 5,5'-dithiobis-(2-nitrobenzoate). In the absence of Na(2)S(2)O(4), ATP and ADP produced changes in sedimentation behaviour and thiol-group reactivity consistent with association of the protein.
mesh_terms: D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D000693:Anaerobiosis; D001565:Benzoates; D001665:Binding Sites; D002850:Chromatography, Gel; D004220:Disulfides; D007501:Iron; D007709:Klebsiella / Q000201:enzymology*; D007711:Klebsiella pneumoniae / Q000201:enzymology; D008667:Metalloproteins; D009574:Nitro Compounds; D009586:Nitrogen Fixation; D010088:Oxidoreductases / Q000032:analysis* / Q000378:metabolism; D011485:Protein Binding; D013438:Sulfhydryl Compounds / Q000032:analysis; D013455:Sulfur; D013997:Time Factors; D014461:Ultracentrifugation
chemical_list: D001565:Benzoates; D004220:Disulfides; D008667:Metalloproteins; D009574:Nitro Compounds; D013438:Sulfhydryl Compounds; D000244:Adenosine Diphosphate; D013455:Sulfur; D000255:Adenosine Triphosphate; D007501:Iron; D010088:Oxidoreductases