Treponeme outer cell envelope: solubilization and reaggregation.
Exposure of the avirulent Kazan 5 treponeme to low concentrations of sodium dodecyl sulfate (1.4 mm) results in the solubilization of the outer envelope. Maximal reaggregation of the outer envelope preparation requires cations, and cation concentration affects both the yield and structure of the aggregates. The reaggregated outer envelope preparation can be resolubilized with the chelating agent, ethylenediaminetetraacetic acid, or with sodium dodecyl sulfate.
D001431:Bacteriological Techniques; D002473:Cell Wall / Q000187:drug effects; D003956:Dialysis; D006018:Glycols / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D008854:Microscopy, Electron; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D012995:Solubility; D014208:Treponema / Q000166:cytology*
D006018:Glycols; D012967:Sodium Dodecyl Sulfate; D008274:Magnesium