Yeast chitin synthetase. Separation of the zymogen from its activating factor and recovery of the latter in the vacuole fraction.
D001391:Azo Compounds; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D002686:Chitin; D003593:Cytoplasm / Q000032:analysis / Q000201:enzymology*; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000302:isolation & purification; D005944:Glucosamine; D005964:Glucosyltransferases / Q000302:isolation & purification*; D006454:Hemoglobins; D008566:Membranes / Q000032:analysis / Q000201:enzymology; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D008928:Mitochondria; D009994:Osmolar Concentration; D009995:Osmosis; D010447:Peptide Hydrolases / Q000302:isolation & purification; D011523:Protoplasts / Q000201:enzymology / Q000378:metabolism; D012260:Ribonucleases / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000032:analysis / Q000166:cytology / Q000201:enzymology*
D001391:Azo Compounds; D004792:Enzyme Precursors; D006454:Hemoglobins; D002686:Chitin; D005964:Glucosyltransferases; D012260:Ribonucleases; D010447:Peptide Hydrolases; D005944:Glucosamine