Initiation factor IF-3-dependent binding of Escherichia coli ribosomes and N-formylmethionine transfer-RNA to rabbit globin messenger.
An initiation complex has been formed in high yields from E. coli ribosomes, 9S messenger RNA for rabbit hemoglobin, and N-formylmethionine-tRNA. Initiation factor IF-3 is required for the binding and puromycin is required for the release of fMet. Valyl-tRNA fails to bind to the second codon, whereas a mixture of 15 aminoacyl-tRNAs promotes incorporation. Together with quantitative data, the findings suggest that IF-3 directs the ribosomes to an AUG codon on one of the two globin messengers, at a site that is different from the normal starting point for globin synthesis.
D000085:Acetates / Q000494:pharmacology; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001426:Bacterial Proteins / Q000494:pharmacology*; D001665:Binding Sites; D002499:Centrifugation, Density Gradient; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005561:Formates / Q000378:metabolism; D005815:Genetic Code; D005914:Globins; D008715:Methionine / Q000378:metabolism; D010442:Peptide Chain Initiation, Translational; D011691:Puromycin / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism* / Q000494:pharmacology; D012343:RNA, Transfer / Q000378:metabolism* / Q000494:pharmacology; D011817:Rabbits; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D014316:Tritium; D014633:Valine / Q000378:metabolism / Q000494:pharmacology
D000085:Acetates; D000596:Amino Acids; D001426:Bacterial Proteins; D005561:Formates; D012333:RNA, Messenger; D014316:Tritium; D011691:Puromycin; D005914:Globins; D012343:RNA, Transfer; D008715:Methionine; D014633:Valine