Replication of bacteriophage M 13: inhibition of single-strand DNA synthesis by rifampicin.
Synthesis of single-stranded DNA of bacteriophage M 13 is blocked by rifampicin. This inhibition is not observed in an Escherichia coli mutant possessing an RNA polymerase resistant to rifampicin. Since rifampicin stops single-strand synthesis faster than chloramphenicol, inhibition by rifampicin does not seem to result from a transcriptional block of protein synthesis, particularly in view of the long half-life of the messenger RNA for M 13. It is, therefore, concluded that the interaction of rifampicin with RNA polymerase directly affects single-strand DNA synthesis. Possible mechanisms are discussed.
D002499:Centrifugation, Density Gradient; D002701:Chloramphenicol / Q000494:pharmacology; D003090:Coliphages / Q000378:metabolism*; D004261:DNA Replication / Q000187:drug effects*; D004277:DNA, Single-Stranded / Q000096:biosynthesis; D004279:DNA, Viral / Q000096:biosynthesis*; D003864:Depression, Chemical; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism; D010759:Phosphorus Isotopes; D012316:RNA Nucleotidyltransferases / Q000378:metabolism; D012293:Rifampin / Q000494:pharmacology*; D014316:Tritium; D014779:Virus Replication / Q000187:drug effects*
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D010759:Phosphorus Isotopes; D014316:Tritium; D002701:Chloramphenicol; D012316:RNA Nucleotidyltransferases; D012293:Rifampin