Isolation and characterization of a lysogenic strain of Nocardia erythropolis.
A stable phage-carrying strain of Nocardia erythropolis was isolated from an infection with the nocardiophage phiEC. Growth of the strain in phage-specific antiserum for 48 hr produced cured organisms at a frequency of about 0.5%. Spontaneous curing, determined by serial single-colony isolations, was less than 0.4%. The strain could not be infected by phage phiEC nor by a closely related phage, phiC, although the cells were able to adsorb these phages. In cell populations, a frequency of 2.5 x 10(-4) cells spontaneously induced. The growth rate of the strain was comparable to that of the uninfected wild-type N. erythropolis. Ultraviolet irradiation or treatment with mitomycin C induced the strain to produce larger numbers of phage. It was concluded that the isolated strain was lysogenic.
D000327:Adsorption; D000818:Animals; D001431:Bacteriological Techniques; D001435:Bacteriophages / Q000254:growth & development*; D007106:Immune Sera / Q000494:pharmacology; D008242:Lysogeny; D008937:Mitomycins / Q000494:pharmacology; D009615:Nocardia / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification / Q000528:radiation effects; D011817:Rabbits / Q000276:immunology; D011830:Radiation Effects; D014466:Ultraviolet Rays
D007106:Immune Sera; D008937:Mitomycins