title: Binucleate classical mycoplasmas pathogenic for goats.
abstract: The growth of three pathogenic goat mycoplasmas, strains Y, KH1 and Mycoplasma mycoides var. capri (PG3), was studied. They formed classical colonies on agar containing 1/500 thallium acetate. They were inactivated during storage at 2 to 4 C and by freezing and thawing but not by shaking. Only KH1 was killed by sonic treatment. Ultraviolet inactivation curves showed that their colony-forming units were single binucleate cells. Details of their growth curves are given. Filtration through 0.45- or 0.3-mum membrane filters removed up to 97% of the cells. Less than 0.003% passed 0.22-mum membranes. In electron micrographs, the cells were seen replicating by budding and most were 0.6 to 0.9 mum in diameter; but cells between 0.1 and 0.2 mum reproduced. They usually multiplied by producing one bud, a form of binary fission. However, two buds were produced by some synchronized cells, indicating that both nuclei had divided simultaneously to form progeny, an alternate method of multiplication.
mesh_terms: D000818:Animals; D002417:Cattle; D002455:Cell Division; D002467:Cell Nucleus; D002470:Cell Survival; D002999:Clone Cells; D003470:Culture Media; D005374:Filtration; D005615:Freezing; D006041:Goats; D008851:Micropore Filters; D008854:Microscopy, Electron; D009174:Mycoplasma / Q000145:classification / Q000166:cytology / Q000254:growth & development* / Q000302:isolation & purification / Q000528:radiation effects; D009176:Mycoplasma mycoides / Q000166:cytology / Q000254:growth & development / Q000302:isolation & purification / Q000528:radiation effects; D011830:Radiation Effects; D014465:Ultrasonics; D014466:Ultraviolet Rays
chemical_list: D003470:Culture Media