Direct demonstration of theta-positive antigen-binding cells, with antigen-induced movement of thymus-dependent cell receptors.
Anti-thetaAKR antibody conjugated to fluorescein has been used in direct immunofluorescence tests to identify spleen theta(+) (T) sheep erythrocyte rosette-forming cells in AKR mice. Specificity studies involving A and cogenic A/thetaAKR mice clearly demonstrated that the cell surface fluorescence and cytotoxicity produced by the antiserum is directed solely toward the thetaAKR alloantigen. Approximately (3/8) of rosette-forming and non-rosette-forming spleen cells were found to be theta(+). The tendency for T cells to bind less antigen and the tendency for antigen-binding T cells to bear less theta than other spleen T cells, first suggested by other studies involving rosette-elimination by anti-thetaC3H plus complement, were confirmed by direct immunofluorescence. All AKR rosettes are specifically inhibitable by anti-immunoglobulin, including T rosettes. Antigen-induced redistribution of T cell receptors, analogous to that previously described for B cell receptors (16), occurs as readily in theta(+)RFC as in theta(-) RFC, without altering the symmetrical ring distribution of thetaAKR antigen.
D000818:Animals; D000906:Antibodies; D000961:Antilymphocyte Serum; D001666:Binding Sites, Antibody; D002462:Cell Membrane / Q000276:immunology; D002859:Chromium Isotopes; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D007104:Immune Adherence Reaction; D007106:Immune Sera; D007136:Immunoglobulins; D007519:Isoantigens; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008806:Mice, Inbred AKR; D008808:Mice, Inbred CBA; D012756:Sheep / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology
D000906:Antibodies; D000961:Antilymphocyte Serum; D002859:Chromium Isotopes; D007106:Immune Sera; D007136:Immunoglobulins; D007519:Isoantigens