Methylation pattern of lambda deoxyribonucleic acid.
Deoxyribonucleic acid (DNA) extracted from phage lambda grown on Escherichia coli K-12 strain W4032 had 113 +/- 10 5-methylcytosine residues and 215 +/- 20 6-methyl adenine residues per genome, as determined by three independent methods. These methylated nucleotides were distributed equally among the two strands of lambda DNA. Shearing of double-stranded DNA to half-length fragments revealed a slight deficiency of 5-methyl cytosine in the 55% guanine plus cytosine half. Shearing the DNA to fragments of smaller length showed that the distribution of methylated nucleotides along the double helix was uniform with the exception of an undermethylated fragment arising from the center of the lambda DNA molecule. The implication of these results for the function of methylated nucleotides in the lambda DNA molecule is discussed.
D000225:Adenine / Q000032:analysis; D001345:Autoradiography; D001483:Base Sequence; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D002855:Chromatography, Thin Layer; D003090:Coliphages / Q000032:analysis* / Q000254:growth & development; D003596:Cytosine / Q000032:analysis; D004277:DNA, Single-Stranded / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D004926:Escherichia coli; D006147:Guanine / Q000032:analysis; D008715:Methionine; D008745:Methylation; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D009711:Nucleotides / Q000032:analysis; D010759:Phosphorus Isotopes; D014316:Tritium
D002247:Carbon Isotopes; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D009711:Nucleotides; D010759:Phosphorus Isotopes; D014316:Tritium; D006147:Guanine; D003596:Cytosine; D008715:Methionine; D000225:Adenine