Effect of cyclopentaneglycine on metabolism in Salmonella typhimurium.
Cyclopentaneglycine (CPG) inhibited the growth of wild-type Salmonella typhimurium. The inhibition was overcome by isoleucine or any isoleucine precursor formed after threonine. CPG appeared to mimic isoleucine as a strong inhibitor of the activity of l-threonine deaminase. The analogue was a poor inhibitor of isoleucyl-transfer ribonucleic acid synthetase. CPG did not appear to be incorporated into protein nor did it replace isoleucine in repression. Cells that had recovered from growth inhibition by CPG had derepressed levels of the isoleucine-valine biosynthetic enzymes.
D000596:Amino Acids / Q000494:pharmacology*; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis; D002247:Carbon Isotopes; D003470:Culture Media; D003517:Cyclopentanes; D004794:Enzyme Repression; D005827:Genetics, Microbial; D005998:Glycine; D006836:Hydro-Lyases / Q000378:metabolism; D007532:Isoleucine / Q000378:metabolism / Q000494:pharmacology; D009154:Mutation; D010649:Phenylalanine / Q000378:metabolism; D012329:RNA, Bacterial / Q000096:biosynthesis; D012486:Salmonella typhimurium / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D013237:Stereoisomerism; D013912:Threonine / Q000378:metabolism; D014498:Uracil / Q000378:metabolism
D000596:Amino Acids; D001426:Bacterial Proteins; D002247:Carbon Isotopes; D003470:Culture Media; D003517:Cyclopentanes; D012329:RNA, Bacterial; D007532:Isoleucine; D013912:Threonine; D010649:Phenylalanine; D014498:Uracil; D006836:Hydro-Lyases; D000604:Amino Acyl-tRNA Synthetases; D005998:Glycine