Replication of bacteriophage phiX-174 in a mutant of Escherichia coli defective in the dnaE gene.
Strains of E. coli bearing a temperature-sensitive mutation at the dnaE locus were unable to support growth of bacteriophage varphiX-174 at 43 degrees while permitting normal growth at 30 degrees . The dnaE gene product was implicated in all stages of varphiX-174 DNA replication. Differences were observed between a double mutant (pol A(-), dnaE(ts)) and a single mutant (pol A(+), dnaE(ts)) in the ability to synthesize parental replicating form and its subsequent replication. The implications of these observations are discussed in terms of the relationship between DNA polymerases-I and -III.
D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D003090:Coliphages / Q000254:growth & development*; D004254:DNA Nucleotidyltransferases / Q000096:biosynthesis / Q000378:metabolism*; D004279:DNA, Viral / Q000032:analysis; D004926:Escherichia coli / Q000201:enzymology*; D005796:Genes; D008242:Lysogeny; D009154:Mutation; D010759:Phosphorus Isotopes; D013696:Temperature; D014316:Tritium; D014779:Virus Replication
D002247:Carbon Isotopes; D004279:DNA, Viral; D010759:Phosphorus Isotopes; D014316:Tritium; D004254:DNA Nucleotidyltransferases