Isolation of substances that stimulate RNA synthesis from red kidney bean: their activities in lymphocytes and Escherichia coli.
An extract from red kidney beans (Phaseolus vulgaris) was fractionated with respect to RNA synthesis-stimulating activity. The activity of the fractions was tested in chicken-spleen lymphocytes cultured in serum-free medium and in plasmolyzed E. coli cells. Lymphocyte-stimulating activity was found in a fraction called F III. This fraction contained protein and orcinol-positive carbohydrate. The protein (called F III p) responsible for the observed activity was separated from the contaminating carbohydrate by acid precipitation. F III p appears to belong to the group of well-known mitogens of high molecular weight present in red kidney beans. A bacteria-stimulating factor was found in a fraction called F V. This factor strongly stimulated RNA synthesis in the bacterial system, and had a weak stimulating effect in the lymphocyte system. In plasmolyzed E. coli cells, F V counteracted the inhibiting effect of rifampicin on RNA synthesis.
D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis; D002645:Chickens; D002701:Chloramphenicol / Q000494:pharmacology; D002850:Chromatography, Gel; D004247:DNA / Q000096:biosynthesis; D004269:DNA, Bacterial / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D037102:Lectins / Q000032:analysis* / Q000302:isolation & purification* / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D009584:Nitrogen / Q000032:analysis; D010429:Pentoses / Q000032:analysis; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D012329:RNA, Bacterial / Q000096:biosynthesis; D012293:Rifampin / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D014316:Tritium
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D037102:Lectins; D010429:Pentoses; D012329:RNA, Bacterial; D014316:Tritium; D012313:RNA; D002701:Chloramphenicol; D004247:DNA; D009584:Nitrogen; D012293:Rifampin