Nuclear deoxyribonucleic acid-dependent ribonucleic acid polymerases from Saccharomyces cerevisiae.
Two deoxyribonucleic acid (DNA)-dependent ribonucleic acid (RNA) polymerases (I, II) have been solubilized from isolated Saccharomyces cerevisiae nuclei. The enzymes can be separated by chromatography on O-diethylaminoethyl Sephadex. Both enzymes are active with high-molecular-weight nuclear yeast DNA, although RNA polymerase I has a higher affinity for polydeoxy-adenylic-thymidylic acid and RNA polymerase II for denatured DNA. RNA polymerase I is active only with manganese. alpha-Amanitin inhibits only the activity of RNA polymerase II.
D002467:Cell Nucleus / Q000032:analysis / Q000201:enzymology; D002845:Chromatography; D002850:Chromatography, Gel; D004247:DNA / Q000302:isolation & purification; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D009183:Mycotoxins / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D012995:Solubility; D013698:Templates, Genetic
D009183:Mycotoxins; D008345:Manganese; D011189:Potassium Chloride; D004247:DNA; D012321:DNA-Directed RNA Polymerases; D008274:Magnesium