Structural changes associated with extraction of group E spore antigen of Clostridium botulinum.
Spores of type E Beluga strain of Clostridium botulinum, which were hydrolyzed with HCl to remove the spore-specific antigen, showed a spore coat which was thinner and projected into the exosporium with multiple protuberances. The changes observed suggest that the antigen is a component of the spore coat connected with the maintenance of rigidity.
D000942:Antigens, Bacterial / Q000302:isolation & purification*; D002473:Cell Wall; D003013:Clostridium / Q000166:cytology*; D003014:Clostridium botulinum / Q000276:immunology; D006851:Hydrochloric Acid; D006868:Hydrolysis; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D009113:Muramidase; D012703:Serotyping; D013170:Spores / Q000166:cytology*; D013171:Spores, Bacterial / Q000166:cytology / Q000276:immunology; D014357:Trypsin
D000942:Antigens, Bacterial; D009113:Muramidase; D014357:Trypsin; D006851:Hydrochloric Acid