title: Messenger ribonucleic acid stability in relaxed and stringent Escherichia coli starved for methionine.
abstract: During starvation for each of four amino acids, relaxed and stringent strains of Escherichia coli showed exponential decay of pulse-labeled unstable messenger ribonucleic acid (mRNA), with RNA degraded more slowly in the relaxed strain. An additional unique difference was observed during starvation for methionine: the relaxed strain showed non-exponential decay of mRNA, with a survival curve similar to that of an aging process.
mesh_terms: D002499:Centrifugation, Density Gradient; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D007532:Isoleucine / Q000378:metabolism; D008715:Methionine / Q000378:metabolism*; D009154:Mutation; D009693:Nucleic Acid Hybridization; D012329:RNA, Bacterial / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012293:Rifampin / Q000494:pharmacology; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D014633:Valine / Q000378:metabolism
chemical_list: D012329:RNA, Bacterial; D012333:RNA, Messenger; D007532:Isoleucine; D014316:Tritium; D008715:Methionine; D014633:Valine; D012293:Rifampin; D014529:Uridine