Temperature-sensitive mutants of Escherichia coli requiring saturated and unsaturated fatty acids for growth: isolation and properties.
A procedure is described for selection of temperature-sensitive mutants affecting fatty-acid synthesis based upon radiation suicide of wild-type organisms by tritiated acetate selectively incorporated into fatty acids. At 37 degrees , two of the mutants extensively incorporate fatty-acid supplements provided in the medium, and grow for extended periods only when a trans-unsaturated or a combination of saturated and cis-unsaturated fatty acids is available. In vivo fatty-acid synthesis, measured by [(14)C]acetate incorporation, is temperature-sensitive in these strains relative to protein synthesis and other non-lipid macromolecular syntheses using acetate. The biochemical nature of these mutations has not been identified.
D000085:Acetates / Q000378:metabolism; D002247:Carbon Isotopes; D004926:Escherichia coli / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D007553:Isotope Labeling; D009154:Mutation; D010714:Phosphatidylethanolamines / Q000032:analysis; D013696:Temperature; D014316:Tritium
D000085:Acetates; D002247:Carbon Isotopes; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D005971:Glutamates; D010714:Phosphatidylethanolamines; D014316:Tritium