Nucleic acid hybridization with RNA immobilized on filter paper.
RNA has been immobilized in a manner suitable for use in molecular hybridization experiments with dissolved RNA or DNA by a nonaqueous solid-phase reaction with carbonyldiimidazole and RNA "dry coated" on cellulose or, preferably, on previously activated phosphocellulose filters. Immobilization of RNA does not appear to alter its chemical character or cause it to acquire affinity for unspecific RNA or DNA. The versatility and efficiency of this method make it potentially attractive for use in routine analytical or preparative hybridization experiments, among other applications.
D002482:Cellulose; D055598:Chemical Phenomena; D002621:Chemistry; D004926:Escherichia coli; D005374:Filtration; D007093:Imidazoles; D007202:Indicators and Reagents; D007659:Ketones; D008722:Methods; D009693:Nucleic Acid Hybridization; D011485:Protein Binding; D012329:RNA, Bacterial
D007093:Imidazoles; D007202:Indicators and Reagents; D007659:Ketones; D012329:RNA, Bacterial; D002482:Cellulose