Screening procedure for Escherichia coli mutants deficient in ribonuclease I.
Strains of Escherichia coli that possess ribonucleic acid accumulated under relaxed growth conditions show a considerable increase in time before the onset of beta-galactosidase inducibility. This time dependency can be related to the presence or absence of ribonuclease I.
D000596:Amino Acids / Q000378:metabolism; D003470:Culture Media; D004790:Enzyme Induction; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D005696:Galactosidases / Q000096:biosynthesis; D005947:Glucose; D008722:Methods; D009154:Mutation; D012329:RNA, Bacterial; D012260:Ribonucleases / Q000096:biosynthesis*; D013997:Time Factors
D000596:Amino Acids; D003470:Culture Media; D012329:RNA, Bacterial; D012260:Ribonucleases; D005696:Galactosidases; D005947:Glucose