Prophage induction by high temperature in thermosensitive dna mutants lysogenic for bacteriophage lambda.
High-temperature treatment of thermosensitive dna mutants lysogenic for phage lambda leads to prophage induction and release of phage (at the permissive temperature) in elongation-defective mutants of the genotypes dnaB, dnaE, and dnaG. In initiation-defective mutants no prophage induction occurs at 42 C in mutants of the genotype dnaA, whereas with a dnaC mutant as well as with strain HfrH 252 (map position not yet known) phages are released at 42 C. DNA degradation at the replication fork at 42 C is observed in all dnaB(lambda) mutants tested, but not in mutants of the genotypes dnaE(lambda) and dnaG(lambda). Therefore, degradation of replication fork DNA is not a prerequisite for prophage induction.
D003090:Coliphages / Q000254:growth & development*; D004261:DNA Replication; D004267:DNA Viruses / Q000254:growth & development*; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004926:Escherichia coli / Q000378:metabolism*; D006358:Hot Temperature; D008242:Lysogeny; D009154:Mutation; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014779:Virus Replication
D004269:DNA, Bacterial; D014316:Tritium; D013936:Thymidine