title: Temperature-sensitive osmotic remedial mutants of Escherichia coli.
abstract: A collection of temperature-sensitive mutants of Escherichia coli K-12 was examined for ability to grow at the restrictive temperature when the osmotic pressure of the medium was increased. Five of the fourteen mutants were found to be osmotic remedial. Four strains containing temperature-sensitive, osmotic-remedial mutations affecting aminoacyl-transfer ribonucleic acid synthetases were found to have altered permeability characteristics which may be attributable to changes in the lipopolysaccharide layer of the cell envelope at restrictive temperatures.
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D001647:Bile Acids and Salts / Q000494:pharmacology; D002473:Cell Wall / Q000378:metabolism; D003470:Culture Media; D003609:Dactinomycin / Q000494:pharmacology; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism; D005827:Genetics, Microbial; D008070:Lipopolysaccharides / Q000378:metabolism; D009154:Mutation; D009995:Osmosis; D009996:Osmotic Fragility; D009997:Osmotic Pressure; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature
chemical_list: D001647:Bile Acids and Salts; D003470:Culture Media; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D003609:Dactinomycin; D012965:Sodium Chloride; D000604:Amino Acyl-tRNA Synthetases