title: Dynamics of insulin release and microtubular-microfilamentous system. I. Effect of cytochalasin B.
abstract: In order to assess the participation of the microfilamentous cell web in the multiphasic response of the pancreatic beta cell, the effect of cytochalasin B upon both glucose- and sulfonylurea-induced insulin release was investigated in the perfused isolated pancreas. Cytochalasin B failed to affect the basal rate of insulin release, but enhanced the initial and later phases of insulin secretion in response to either glucose or gliclazide. In addition, cytochalasin B lowered the threshold concentration for the stimulant action of glucose upon insulin release. Ultrastructural studies supported the concept of a specific interaction of cytochalasin B with the microfilamentous cell web of the beta cell. It is concluded that the integrity of such a structure is equally important for both the initial and later secretory responses of the beta cell to various insulinotropic agents.
mesh_terms: D000818:Animals; D003571:Cytochalasin B / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D007211:Indoles / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D008870:Microtubules / Q000187:drug effects*; D003904:Mitosporic Fungi; D010477:Perfusion; D051381:Rats; D013453:Sulfonylurea Compounds / Q000494:pharmacology
chemical_list: D007211:Indoles; D007328:Insulin; D013453:Sulfonylurea Compounds; D003571:Cytochalasin B; D005947:Glucose