Insertion of the F factor into the cluster of rfa (rough A) genes of Salmonella typhimurium.
From F(+) strains of Salmonella typhimurium, isolates were obtained representing two new classes of Hfr strains, HfrK1 and HfrK2, in which the insertion of the F factor into the rfa genes results in chromosome mobilization either clockwise or anticlockwise from rfa, and in the Rfa phenotype. The point of insertion of the F factor into the cluster of rfa genes, revealed by studies of the early transfer of their normal alleles, is as follows: xyl-cysE-rfa-657 (HfrK2-1, SA540 -->)-(<-- HfrK1-1, SA458)-rfaG-(<-- HfrK1-2, SA464)-pyrE-metA
D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003227:Conjugation, Genetic; D003433:Crosses, Genetic; D004269:DNA, Bacterial; D005796:Genes; D008040:Genetic Linkage; D008070:Lipopolysaccharides / Q000096:biosynthesis*; D008242:Lysogeny; D009154:Mutation; D010641:Phenotype; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D012343:RNA, Transfer; D012483:Salmonella Phages; D012486:Salmonella typhimurium / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*
D004269:DNA, Bacterial; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D012343:RNA, Transfer