Requirement for alpha-L-fucose on the macrophage membrane receptor for MIF.
alpha-L-fucose abolishes the activity of guinea pig migration inhibitory factor (MIF) on the macrophages. Other sugars such as alpha-D-glucose, beta-D-galactose, alpha-L-rhamnose, methyl-alpha-D-mannoside, and N-acetyl-beta-D-glucosamine had no effect. Theabolition of MIF activity by alpha-L-fucose was reversible. When macrophages were incubated with alpha-L-fucosidase, a glycosidase which splits terminal alpha-L-fucose from oligosaccharides, the macrophages no longer responded to MIF. On the other hand, MIF incubated with alpha-L-fucosidase was still active. These experiments strongly suggest that alpha-L-fucose comprises an essential part of a macrophage membrane receptor for MIF.
D000818:Animals; D001665:Binding Sites; D002462:Cell Membrane / Q000276:immunology*; D005643:Fucose / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000494:pharmacology; D006168:Guinea Pigs; D008263:Macrophage Migration-Inhibitory Factors / Q000032:analysis / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D009005:Monosaccharides / Q000494:pharmacology
D008263:Macrophage Migration-Inhibitory Factors; D009005:Monosaccharides; D005643:Fucose; D006026:Glycoside Hydrolases