Strand-specific nick in open circular R-factor deoxyribonucleic acid: attachment of the linear strand to a proteinaceous cellular component.
The fraction of covalently closed circular R-factor R12 deoxyribonucleic acid (DNA) isolated from Proteus mirabilis was found to depend upon the pH of the buffer used to suspend the cells at the time of lysis. Examination of the open circular DNA revealed that there is one strand-specific nick per R-factor molecule. This same DNA strand also appeared to be bound to a proteinaceous particulate fraction of the cell.
D001426:Bacterial Proteins / Q000032:analysis*; D002499:Centrifugation, Density Gradient; D004269:DNA, Bacterial / Q000032:analysis* / Q000302:isolation & purification; D004270:DNA, Circular / Q000032:analysis / Q000302:isolation & purification; D004277:DNA, Single-Stranded / Q000032:analysis / Q000302:isolation & purification; D004352:Drug Resistance, Microbial; D005111:Extrachromosomal Inheritance; D006863:Hydrogen-Ion Concentration; D008722:Methods; D011513:Proteus mirabilis / Q000032:analysis / Q000254:growth & development; D013395:Sucrose
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004270:DNA, Circular; D004277:DNA, Single-Stranded; D013395:Sucrose