DNA modification methylase activity of Escherichia coli restriction endonucleases K and P.
The highly purified restriction endonucleases of E. coli K and coliphage P1 transfer methyl groups from S-adenosylmethionine to adenine residues of unmodified DNA. Incubation of unmodified DNA with endonucleases K or P and S-adenosylmethionine renders the DNA resistant to restriction. The enzymes, therefore, have both restriction endonuclease and modification methylase activities.
D002848:Chromatography, DEAE-Cellulose; D003090:Coliphages / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D008745:Methylation; D008780:Methyltransferases / Q000378:metabolism*; D010759:Phosphorus Isotopes; D012436:S-Adenosylmethionine / Q000378:metabolism; D014316:Tritium
D004279:DNA, Viral; D010759:Phosphorus Isotopes; D014316:Tritium; D012436:S-Adenosylmethionine; D008780:Methyltransferases; D004720:Endonucleases