RNA-linked DNA fragments in vitro.
RNA-linked DNA fragments are intermediates in DNA replication in Escherichia coli cells made permeable to nucleoside triphosphates by treatment with toluene. Covalent linkage of a short RNA stretch to the 5' end of the DNA is proved by transfer of (32)P from [alpha-(32)P]dNTP to ribonucleotides upon digestion with alkali or pancreatic RNase, and by a small decrease in the molecular size upon alkaline hydrolysis. The (32)P transfer experiments reveal a unique structure...p(rPy)p(rA)p(rU or rC)p(dC)p... at the RNA-DNA junction.
D000255:Adenosine Triphosphate / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D002499:Centrifugation, Density Gradient; D002848:Chromatography, DEAE-Cellulose; D003597:Cytosine Nucleotides / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects; D004269:DNA, Bacterial / Q000032:analysis / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010759:Phosphorus Isotopes; D012329:RNA, Bacterial / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism; D013942:Thymine Nucleotides / Q000378:metabolism; D014050:Toluene / Q000494:pharmacology; D014316:Tritium
D003597:Cytosine Nucleotides; D004269:DNA, Bacterial; D010759:Phosphorus Isotopes; D012329:RNA, Bacterial; D013942:Thymine Nucleotides; D014316:Tritium; D014050:Toluene; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D012260:Ribonucleases