Cultivation of parasitic leptospires: effect of pyruvate.
Sodium pyruvate (100 mug/ml) is a useful addition to the Tween 80-albumin medium for the cultivation of parasitic serotypes. It is most effective in promoting growth from small inocula and growth of the nutritionally fastidious serotypes.
D000818:Animals; D001431:Bacteriological Techniques; D006224:Cricetinae; D003470:Culture Media; D007919:Leptospira / Q000187:drug effects / Q000254:growth & development* / Q000472:pathogenicity; D041341:Leptospira interrogans serovar canicola / Q000187:drug effects / Q000254:growth & development / Q000472:pathogenicity; D007928:Lethal Dose 50; D008297:Male; D011773:Pyruvates / Q000494:pharmacology*; D012703:Serotyping; D012710:Serum Albumin, Bovine; D012964:Sodium; D013045:Species Specificity; D013501:Surface-Active Agents; D014774:Virulence
D003470:Culture Media; D011773:Pyruvates; D013501:Surface-Active Agents; D012710:Serum Albumin, Bovine; D012964:Sodium