title: An RNA that multiplies indefinitely with DNA-dependent RNA polymerase: selection from a random copolymer.
abstract: We have selected from a mixture of random polynucleotides an RNA that is able to replicate in the presence of the DNA-dependent RNA polymerase of Escherichia coli, provided the medium contains Mn(2+) and ITP, in addition to ATP, CTP, and UTP. The RNA consists of a chain of about 125 (+/-25) nucleotides and appears to have a defined, nonrepetitive sequence.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D002247:Carbon Isotopes; D003597:Cytosine Nucleotides / Q000378:metabolism; D004247:DNA; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D007292:Inosine Nucleotides / Q000378:metabolism; D008345:Manganese / Q000494:pharmacology; D009711:Nucleotides / Q000032:analysis; D010759:Phosphorus Isotopes; D012313:RNA / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism; D013698:Templates, Genetic; D014316:Tritium; D014500:Uracil Nucleotides / Q000378:metabolism
chemical_list: D002247:Carbon Isotopes; D003597:Cytosine Nucleotides; D007292:Inosine Nucleotides; D009711:Nucleotides; D010759:Phosphorus Isotopes; D014500:Uracil Nucleotides; D014316:Tritium; D008345:Manganese; D012313:RNA; D000255:Adenosine Triphosphate; D004247:DNA; D012321:DNA-Directed RNA Polymerases; D012260:Ribonucleases