Identification of the block in the intracellular replication of single-stranded DNA of photodynamically inactivated bacteriophage phi X174.
(32)P-labeled single-stranded DNA phage phiX174 was photodynamically inactivated by irradiation in air with visible light in the presence of the acridine dye, proflavine sulfate. The inactivated phages could adsorb to the host cells but failed to lyse them. Formation of intracellular mature phages was almost completely inhibited. Photodynamic lesions in phiX174 DNA caused intracellular formation of defective double-stranded replicative form molecules which ultimately reverted to the single-stranded configuration.
D000166:Acridines; D000327:Adsorption; D002845:Chromatography; D003090:Coliphages / Q000032:analysis / Q000254:growth & development / Q000378:metabolism*; D004261:DNA Replication; D004267:DNA Viruses / Q000032:analysis / Q000254:growth & development / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000032:analysis / Q000096:biosynthesis; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis*; D004926:Escherichia coli; D006882:Hydroxyapatites; D008027:Light; D008242:Lysogeny; D010759:Phosphorus Isotopes
D000166:Acridines; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D006882:Hydroxyapatites; D010759:Phosphorus Isotopes