title: Modification of the gelatin substrate procedure for demonstration of acrosomal proteolytic activity.
mesh_terms: D000818:Animals; D001345:Autoradiography; D002417:Cattle; D003670:Deer; D005557:Formaldehyde / Q000494:pharmacology; D005780:Gelatin / Q000378:metabolism*; D006168:Guinea Pigs; D006651:Histocytochemistry; D006736:Horses; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007531:Isoflurophate / Q000494:pharmacology; D008297:Male; D008567:Membranes, Artificial; D008722:Methods; D051379:Mice; D008858:Microscopy, Phase-Contrast; D010447:Peptide Hydrolases / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D013094:Spermatozoa / Q000201:enzymology*; D014105:Tosyl Compounds / Q000494:pharmacology; D014238:Trichloroacetic Acid / Q000494:pharmacology; D014316:Tritium; D014361:Trypsin Inhibitors; D014508:Urea / Q000494:pharmacology
chemical_list: D008567:Membranes, Artificial; D014105:Tosyl Compounds; D014361:Trypsin Inhibitors; D014316:Tritium; D007531:Isoflurophate; D005557:Formaldehyde; D014238:Trichloroacetic Acid; D014508:Urea; D005780:Gelatin; D010447:Peptide Hydrolases