Asymmetric transcription of bacteriophage Mu-1.
The deoxyribonucleic acid (DNA) of bacteriophage Mu-1 can be separated into its complementary strands by poly(U,G) binding and equilibrium centrifugation. DNA-ribonucleic acid (RNA) hybridizations in liquid show that more than 98% of "early" phage-specific RNA and over 96% of "late" messenger species bind to the heavy [poly(U,G)-binding] strand of Mu-1 DNA. A small (1.45%) but significant amount of late RNA binds to the light strand. The significance of this RNA fraction is discussed in connection with the peculiar structure of denatured and reannealed Mu-1 DNA.
D002499:Centrifugation, Density Gradient; D003090:Coliphages / Q000254:growth & development / Q000378:metabolism*; D004279:DNA, Viral / Q000302:isolation & purification; D004926:Escherichia coli; D008242:Lysogeny; D008854:Microscopy, Electron; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009695:Nucleic Acid Renaturation; D011119:Polynucleotides; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D013997:Time Factors; D014158:Transcription, Genetic
D004279:DNA, Viral; D011119:Polynucleotides; D012333:RNA, Messenger; D012367:RNA, Viral