Acyl carrier protein in mycoplasmas.
Acyl carrier protein (ACP) activity was determined by the malonyl-coenzyme A-CO(2) exchange reaction. It was highest in Acholeplasma laidlawii, lower in A. granularum, and lowest in A. axanthum. The sterol-requiring Mycoplasma species examined showed little or negligible ACP activity. A. laidlawii was capable of utilizing pantetheine or coenzyme A but not beta-alanine as precursor for ACP synthesis. Its ACP could thus be labeled by growing the organisms with radioactive coenzyme A. The ACP of A. laidlawii appears to be a soluble cytoplasmic protein, which could be purified about 40-fold by treatment of the cytoplasmic fluid with streptomycin sulfate and chromatography of the supernatant fluid on a Biogel P-10 column. Its molecular weight, determined by polyacrylamide gel electrophoresis, is low (about 10,900) resembling that of Escherichia coli, but it is much more sensitive to heat.
D000085:Acetates / Q000378:metabolism; D000128:Acholeplasma laidlawii / Q000032:analysis / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D000215:Acylation; D000409:Alanine / Q000378:metabolism; D002247:Carbon Isotopes; D002352:Carrier Proteins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D002845:Chromatography; D003065:Coenzyme A / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000032:analysis; D005227:Fatty Acids / Q000096:biosynthesis; D008970:Molecular Weight; D009174:Mycoplasma / Q000032:analysis* / Q000378:metabolism; D009176:Mycoplasma mycoides / Q000032:analysis / Q000378:metabolism; D010205:Pantothenic Acid / Q000378:metabolism; D011498:Protein Precursors / Q000494:pharmacology; D013045:Species Specificity; D013696:Temperature
D000085:Acetates; D002247:Carbon Isotopes; D002352:Carrier Proteins; D005227:Fatty Acids; D011498:Protein Precursors; D010205:Pantothenic Acid; D000409:Alanine; D003065:Coenzyme A