A simple method of preparing large amounts of phiX174 RF 1 supercoiled DNA.
D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D003090:Coliphages; D004279:DNA, Viral / Q000096:biosynthesis / Q000302:isolation & purification*; D004926:Escherichia coli / Q000378:metabolism; D000431:Ethanol; D007553:Isotope Labeling; D008722:Methods; D010710:Phosphates / Q000378:metabolism; D010759:Phosphorus Isotopes; D012965:Sodium Chloride; D012967:Sodium Dodecyl Sulfate; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium
D004279:DNA, Viral; D010710:Phosphates; D010759:Phosphorus Isotopes; D014316:Tritium; D012967:Sodium Dodecyl Sulfate; D000431:Ethanol; D012965:Sodium Chloride; D013936:Thymidine