Involvement of threonine deaminase in repression of the isoleucine-valine and leucine pathways in Saccharomyces cerevisiae.
l-Threonine deaminase (l-threonine dehydratase [deaminating], EC 4.2.2.16) has been shown to be involved in the regulation of three of the enzymes of isoleucine-valine biosynthesis in yeast. Mutations affecting the affinity of the enzyme for isoleucine also affected the repression of acetohydroxyacid synthase, dihydroxyacid dehydrase, and reductoisomerase. The data indicate that isoleucine must be bound for effective repression of these enzymes to take place. In a strain with a nonsense mutation midway in liv 1, the gene for threonine deaminase, starvation for isoleucine or valine did not lead to derepression of the three enzymes; starvation for leucine did. The effect of the nonsense mutation is recessive; it is tentatively concluded, therefore, that intact threonine deaminase is required for derepression by two of the effectors for multivalent repression, but not by the third. A model is presented which proposes that a regulatory species of leu tRNA(leu) is the key intermediate for repression and that threonine deaminase is a positive element, regulating the available pool of charged leu tRNA by binding it.
D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D004794:Enzyme Repression; D006836:Hydro-Lyases / Q000096:biosynthesis / Q000378:metabolism; D007532:Isoleucine / Q000096:biosynthesis* / Q000378:metabolism; D007535:Isomerases / Q000096:biosynthesis / Q000378:metabolism; D007930:Leucine / Q000096:biosynthesis* / Q000378:metabolism; D008190:Lyases / Q000096:biosynthesis / Q000378:metabolism; D008956:Models, Chemical; D009154:Mutation; D011773:Pyruvates; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D014633:Valine / Q000096:biosynthesis* / Q000378:metabolism
D003470:Culture Media; D011773:Pyruvates; D007532:Isoleucine; D008190:Lyases; D006836:Hydro-Lyases; D007535:Isomerases; D007930:Leucine; D014633:Valine