Mannitol sensitivity.
The addition of mannitol to cultures of Salmonella typhimurium mutants missing mannitol-1-phosphate dehydrogenase causes stasis or lysis. Mannitol-1-phosphate accumulates intracellularly to concentrations of 20 mm. The incorporation of acetate into lipid is inhibited before cell wall, protein, or nucleic acid synthesis.
D000085:Acetates / Q000037:antagonists & inhibitors; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis; D001433:Bacteriolysis; D002247:Carbon Isotopes; D002473:Cell Wall / Q000378:metabolism; D002854:Chromatography, Paper; D005827:Genetics, Microbial; D008055:Lipids / Q000096:biosynthesis; D008353:Mannitol / Q000378:metabolism / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009154:Mutation; D009696:Nucleic Acids / Q000096:biosynthesis; D010649:Phenylalanine / Q000378:metabolism; D010867:Pimelic Acids / Q000378:metabolism; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D013237:Stereoisomerism; D013997:Time Factors; D014316:Tritium; D014498:Uracil / Q000378:metabolism
D000085:Acetates; D001426:Bacterial Proteins; D002247:Carbon Isotopes; D008055:Lipids; D009696:Nucleic Acids; D010867:Pimelic Acids; D014316:Tritium; D008353:Mannitol; D010649:Phenylalanine; D014498:Uracil; D000429:Alcohol Oxidoreductases