Primary structure of neocarzinostatin, an antitumor protein.
The antitumor protein neocarzinostatin is an acidic single-chain molecule, cross-linked by two disulfide bridges, and consists of 109 amino acid residues. Complete disulfide bond reduction and S-carboxymethylation was achieved in liquid ammonia. Sequence determination of five tryptic fragments led to the proposed primary structure.
D000595:Amino Acid Sequence; D000903:Antibiotics, Antineoplastic; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D002918:Chymotrypsin; D004590:Electrophoresis, Paper; D010434:Pepsin A; D010455:Peptides / Q000032:analysis; D011506:Proteins; D013820:Thermolysin
D000903:Antibiotics, Antineoplastic; D010455:Peptides; D011506:Proteins; D002918:Chymotrypsin; D010434:Pepsin A; D013820:Thermolysin