New medium for the isolation and enumeration of pseudomonads.
A new medium containing 200 ppb of 2-hydroxy-2',4,4'-trichlorodiphenyloxide (CH3565) and 10 ppm of cetyl-trimethyl-ammonium bromide (Cetrimide) in tryptic soy agar was developed and tested with 19 pure cultures of Pseudomonas, 20 microorganisms of other genera, commercially prepared ground beef, and laboratory-prepared inoculated ground beef. The new medium, CETCH agar, was compared with an antibiotic-containing medium. CETCH agar provided greater pseudomonad recoveries, a shorter incubation period prior to plate counting, and greater ease of preparation.
D000362:Agar; D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification; D001431:Bacteriological Techniques; D001965:Bromides; D003470:Culture Media; D005516:Food Microbiology; D005519:Food Preservation; D008460:Meat; D011549:Pseudomonas / Q000254:growth & development / Q000302:isolation & purification*; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents
D001965:Bromides; D003470:Culture Media; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents; D000362:Agar