Influence of thymine starvation on the integrity of episomal and chromosomal deoxyribonucleic acids in Escherichia coli CR34 (lambda ind-).
The effect of thymine deprivation on the integrity of phage lambda, sex factor, and chromosomal deoxyribonucleic acid (DNA) in Escherichia coli CR34 (lambda ind(-)) was examined by sedimenting cell lysates through alkaline sucrose gradients. Both sex factor and chromosomal DNAs showed evidence of being degraded during the starvation period. In contrast, no loss of closed circular lambda DNA was observed.
D002250:Carbon Radioisotopes; D002499:Centrifugation, Density Gradient; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D005111:Extrachromosomal Inheritance; D012737:Sex Factors; D013395:Sucrose; D013941:Thymine / Q000378:metabolism*; D014316:Tritium
D002250:Carbon Radioisotopes; D004269:DNA, Bacterial; D014316:Tritium; D013395:Sucrose; D013941:Thymine