Three-dimensional structure of yeast phenylalanine transfer RNA: folding of the polynucleotide chain.
At 4 A resolution the polynucleotides in yeast phenylalanine transfer RNA are seen in a series of electron dense masses about 5.8 A apart. These peaks are probably associated with the phosphate groups, while lower levels of electron density between segments of adjacent polynucleotide chains are interpreted as arising from hydrogen-bonded purine-pyrimidine base pairs. It is possible to trace the entire polynucleotide chain with only two minor regions of ambiguity. The polynucleotide chain has a secondary structure consistent with the cloverleaf conformation; however, its folding is different from that proposed in any model. The molecule is made of two double-stranded helical regions oriented at right angles to each other in the shape of an L. One end of the L has the CCA acceptor; the anticodon loop is at the other end, and the dihydrouridine and TpsiC loops form the corner.
D001483:Base Sequence; D008961:Models, Structural; D009690:Nucleic Acid Conformation; D010649:Phenylalanine; D011119:Polynucleotides / Q000032:analysis; D012343:RNA, Transfer; D012441:Saccharomyces cerevisiae / Q000032:analysis*; D014961:X-Ray Diffraction
D011119:Polynucleotides; D010649:Phenylalanine; D012343:RNA, Transfer