title: Macromolecular synthesis during the germanation of Saccharomyces cerevisiae spores.
abstract: After the dormancy of Saccharomyces cerevisiae ascospores had been broken, the synthesis of proteins was observed first, followed rapidly by synthesis of ribonucleic acid (RNA) and much later by deoxyribonucleic acid (DNA) synthesis. Phosphoglucomutase activity increased in a periodic (step) fashion, whereas the activity of five other enzymes increased linearly during germination and outgrowth. The rate of synthesis of these enzymes was highest at about the period of DNA replication. The amino acid pools of dormant spores contained high levels of proline, glutamic acid, and histidine. At 2 h after onset of germination, the pools of phenylalanine and methionine had disappeared and the other components had decreased significantly. By 3.5 h, with the exception of proline and cystine, most amino acid pool components had significantly increased.
mesh_terms: D000225:Adenine / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D001224:Aspartic Acid; D002247:Carbon Isotopes; D002474:Cell-Free System; D003596:Cytosine / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis*; D005656:Fungal Proteins / Q000096:biosynthesis*; D005956:Glucose-6-Phosphate Isomerase / Q000096:biosynthesis; D005954:Glucosephosphate Dehydrogenase / Q000096:biosynthesis; D006593:Hexokinase / Q000096:biosynthesis; D010733:Phosphoglucomutase / Q000096:biosynthesis; D010734:Phosphogluconate Dehydrogenase / Q000096:biosynthesis; D010770:Phosphotransferases / Q000096:biosynthesis; D012313:RNA / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D013170:Spores / Q000378:metabolism*; D013172:Spores, Fungal / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D013997:Time Factors; D014316:Tritium; D014498:Uracil / Q000378:metabolism
chemical_list: D000596:Amino Acids; D002247:Carbon Isotopes; D005656:Fungal Proteins; D014316:Tritium; D001224:Aspartic Acid; D014498:Uracil; D012313:RNA; D003596:Cytosine; D004247:DNA; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D010770:Phosphotransferases; D006593:Hexokinase; D005956:Glucose-6-Phosphate Isomerase; D010733:Phosphoglucomutase; D000225:Adenine