title: Negative control of protein synthesis after infection with bacteriophage T7.
abstract: T7 phage induces two negative control mechanisms of protein synthesis: (a) Host-gene expression is repressed by a "T7 repressor," and (b) early T7 protein synthesis is inhibited by a late phage protein.(a) The repressor for host enzyme synthesis is an early T7 protein. Its gene is none of the known early genes; it is located promotor-proximal to gene 1. The repressor function of this protein can be demonstrated by DNA-dependent enzyme synthesis in vitro.(b) Expression of early phage gene is depressed by a late phage protein or by T7 RNA polymerase. Control takes place on the level of transcription.
mesh_terms: D001426:Bacterial Proteins / Q000037:antagonists & inhibitors*; D003090:Coliphages / Q000378:metabolism*; D004279:DNA, Viral; D004790:Enzyme Induction; D004794:Enzyme Repression; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism; D005696:Galactosidases / Q000096:biosynthesis; D007700:Kinetics; D008025:Ligases / Q000096:biosynthesis; D008722:Methods; D011119:Polynucleotides; D012316:RNA Nucleotidyltransferases / Q000096:biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification; D012436:S-Adenosylmethionine; D013997:Time Factors; D014166:Transferases / Q000096:biosynthesis; D014764:Viral Proteins / Q000096:biosynthesis*
chemical_list: D001426:Bacterial Proteins; D004279:DNA, Viral; D011119:Polynucleotides; D012333:RNA, Messenger; D014764:Viral Proteins; D012436:S-Adenosylmethionine; D014166:Transferases; D012316:RNA Nucleotidyltransferases; D005696:Galactosidases; D008025:Ligases