New chromatographic form of phenylalanine transfer ribonucleic acid from Escherichia coli growing exponentially in a low-phosphate medium.
Reversed-phase chromatography has been used to detect the presence of a new form of phenylalanyl-transfer ribonucleic acid (Phe-tRNA) from Escherichia coli growing exponentially in media containing low but nonlimiting levels of inorganic phosphate. The amount of this extra Phe-tRNA form is greatest in slowly growing cells (0.8 generations/h), and becomes negligible in media supporting a rapid growth rate (2.14 generations/h).
D002250:Carbon Radioisotopes; D002845:Chromatography; D003470:Culture Media; D004926:Escherichia coli / Q000032:analysis* / Q000254:growth & development / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D010649:Phenylalanine / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism*; D012329:RNA, Bacterial / Q000032:analysis*; D012343:RNA, Transfer / Q000032:analysis*; D013053:Spectrophotometry; D013386:Succinates / Q000378:metabolism; D014316:Tritium
D002250:Carbon Radioisotopes; D003470:Culture Media; D010710:Phosphates; D012329:RNA, Bacterial; D013386:Succinates; D014316:Tritium; D010649:Phenylalanine; D012343:RNA, Transfer; D005947:Glucose; D005990:Glycerol