title: Localization of two functions of the phosphoribosyl anthranilate transferase of Escherichia coli to distinct regions of the polypeptide chain.
abstract: The trpD gene specifies a polypeptide which has both glutamine amidotransferase and phosphoribosyl anthranilate (PRA) transferase activities. Deletions fusing segments of trpD to the gene preceding it in the operon, trpE, were selected in strains carrying various trpD point mutations. The selection procedure required both that a deletion enter trpE and that it restore the PRA transferase activity which the parent trpD point mutant lacked. Deletion mutants were found which had PRA transferase activity although the first third of trpD was deleted. The existence of the mutants proves that a terminal segment of trpD is sufficient to specify a polypeptide having PRA transferase activity. The location of the deletion end points on the genetic map of trpD defines the extent of the trpD segment required for PRA transferase activity. This segment did not overlap the initial region of trpD required to specify the glutamine amidotransferase function of the trpD polypeptide. These results support the hypothesis (M. Grieshaber and R. Bauerle, 1972; H. Zalkin and L. H. Hwang, 1971) that the bifunctional trpD polypeptide might have evolved by fusion of a gene specifying a glutamine amidotransferase with a gene directing PRA transferase synthesis.
mesh_terms: D000641:Ammonia / Q000378:metabolism; D000878:Anthranilate Synthase / Q000378:metabolism; D002474:Cell-Free System; D002874:Chromosome Mapping; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D005796:Genes; D005973:Glutamine / Q000378:metabolism; D009097:Multienzyme Complexes / Q000378:metabolism; D009154:Mutation; D010430:Pentosyltransferases / Q000378:metabolism; D011995:Recombination, Genetic; D000637:Transaminases / Q000378:metabolism; D014161:Transduction, Genetic; D014364:Tryptophan / Q000096:biosynthesis; D014367:Tryptophan Synthase / Q000378:metabolism; D062367:ortho-Aminobenzoates / Q000378:metabolism
chemical_list: D009097:Multienzyme Complexes; D062367:ortho-Aminobenzoates; D005973:Glutamine; D000641:Ammonia; D014364:Tryptophan; D010430:Pentosyltransferases; D000637:Transaminases; D000878:Anthranilate Synthase; D014367:Tryptophan Synthase