title: Detection of viral DNA sequences in adenovirus-transformed cells by in situ hybridization.
abstract: Cytological preparations of cells transformed by members of three groups of human adenoviruses, adenovirus 12, 7, and 2, were annealed with radioactive complementary RNA (cRNA) (4 x 10(7) to 4.5 x 10(7) dpm/mug) prepared by copying viral DNA with the Escherichia coli DNA-directed RNA polymerase. These in situ hybridizations detected adenovirus-specific DNA sequences in interphase nuclei when transformed cells were annealed with homologous viral cRNA, but not with heterologous viral cRNA. The highest autoradiographic grain counts were found over adenovirus 7-transformed cell nuclei, next over adenovirus 12-, and the lowest over adenovirus 2-transformed cell nuclei. This is the same order as found by reassociation kinetic measurements (K. Fujinaga and M. Green, unpublished data).
mesh_terms: D000256:Adenoviridae / Q000032:analysis*; D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002474:Cell-Free System; D002999:Clone Cells; D006224:Cricetinae; D004279:DNA, Viral / Q000032:analysis*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology; D008722:Methods; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D013698:Templates, Genetic; D014158:Transcription, Genetic; D014316:Tritium; D014500:Uracil Nucleotides / Q000378:metabolism
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D014500:Uracil Nucleotides; D014316:Tritium; D012321:DNA-Directed RNA Polymerases