The thermosensitive lesion in the replication of the drug resistance factor, Rts1.
The DNA of the thermosensitive R factor, Rts1, has been examined by the technique of sedimentation in alkaline sucrose density gradients. Rts1 DNA was found as closed covalent circles in only a few copies per cell in an Escherichia coli host at the permissive temperature. Rts1 DNA appears to be synthesized at the nonpermissive temperature, but was not found as closed covalent circles. However, circular DNA could be recovered upon shift down to the permissive temperature. The large number of plasmid-negative cells which accumulate after prolonged culture at non-permissive temperature may be due to a strong selective pressure favoring the growth of rare R(-) segregants.
D002499:Centrifugation, Density Gradient; D004261:DNA Replication; D004269:DNA, Bacterial / Q000302:isolation & purification; D004270:DNA, Circular / Q000302:isolation & purification; D004926:Escherichia coli / Q000032:analysis* / Q000378:metabolism; D005111:Extrachromosomal Inheritance; D007612:Kanamycin; D008970:Molecular Weight; D010403:Penicillin Resistance; D010406:Penicillins; D013696:Temperature
D004269:DNA, Bacterial; D004270:DNA, Circular; D010406:Penicillins; D007612:Kanamycin