Tissue-specific transcription of the globin gene in isolated chromatin.
RNA was transcribed from chrmation from mouse fetal liver or brain, by use of DNA-dependent RNA polymerase of Escherichia coli. Globin messenger RNA sequences in the transcript were measured with complementary DNA copied from globin messenger RNA with RNA-dependent DNA polymerase. Globin messenger RNA sequences were found in RNA newly transcribed from chromatin from erythropoietic tissue but not in that transcribed from brain chromatin.
D000818:Animals; D001923:Brain Chemistry; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D002843:Chromatin / Q000302:isolation & purification / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D005333:Fetus; D005914:Globins; D008099:Liver / Q000032:analysis / Q000378:metabolism; D051379:Mice; D009693:Nucleic Acid Hybridization; D010761:Phosphorus Radioisotopes; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D013698:Templates, Genetic; D014158:Transcription, Genetic; D014316:Tritium
D002843:Chromatin; D010761:Phosphorus Radioisotopes; D012333:RNA, Messenger; D014316:Tritium; D005914:Globins; D004247:DNA; D012321:DNA-Directed RNA Polymerases