Purification of Clostridium botuliunum type F progenitor  toxin.
Clostridium botulinum type F progenitor toxin was purified to a homogeneous state as judged by gel filtration on Sephadex G-200, ultracentrifugation, and disc electrophoresis. The sedimentation constant, corrected to water at 20 C, of type F progenitor toxin was determined to be 10.3 and the molecular weight to be 235,000 by ultracentrifugation at pH 6.0. The purified toxin contained a toxicity of 1.2 x 10(8) 50% lethal doses/mg of N. In agar gel double diffusion, it formed two precipitin lines at pH 6.0. The progenitor toxin of type F differs from that of type A in that it contains no hemagglutinin and from that of type E in that it is not activable.
D000645:Ammonium Sulfate; D000818:Animals; D001905:Botulinum Toxins / Q000032:analysis / Q000302:isolation & purification* / Q000633:toxicity; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D003014:Clostridium botulinum / Q000032:analysis*; D004586:Electrophoresis; D007274:Injections, Intraperitoneal; D007928:Lethal Dose 50; D051379:Mice; D008970:Molecular Weight; D011479:Protamines; D012703:Serotyping; D012965:Sodium Chloride; D013056:Spectrophotometry, Ultraviolet; D014461:Ultracentrifugation
D011479:Protamines; D012965:Sodium Chloride; D001905:Botulinum Toxins; D000645:Ammonium Sulfate