Use of mucoid bacterial mutants to circumvent clumping of cells and minicells containing R plasmids derepressed for pilus synthesis.
Clumping of bacteria containing R factors derepressed for pilus synthesis interfered with the separation of cells and minicells. Mucoid derivatives of these bacteria provided the means of preventing clumping and allowing a good separation. This method may be generally useful in dealing with autoagglutination of bacterial cultures.
D000371:Agglutination; D001426:Bacterial Proteins / Q000096:biosynthesis; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D003090:Coliphages; D004267:DNA Viruses; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism; D005111:Extrachromosomal Inheritance; D008242:Lysogeny; D009154:Mutation
D001426:Bacterial Proteins