Stimulation of lysine decarboxylase production in Escherichia coli by amino acids and peptides.
A commercial hydrolysate of casein stimulated production of lysine decarboxylase (EC 4.1.1.18) by Escherichia coli B. Cellulose and gel chromatography of this hydrolysate yielded peptides which were variably effective in this stimulation. Replacement of individual, stimulatory peptides by equivalent amino acids duplicated the enzyme levels attained with those peptides. There was no indication of specific stimulation by any peptide. The peptides were probably taken up by the oligopeptide transport system of E. coli and hydrolyzed intracellularly by peptidases to their constituent amino acids for use in enzyme synthesis. Single omission of amino acids from mixtures was used to screen them for their relative lysine decarboxylase stimulating abilities. Over 100 different mixtures were evaluated in establishing the total amino acid requirements for maximal synthesis of lysine decarboxylase by E. coli B. A mixture containing all of the common amino acids except glutamic acid, aspartic acid, and alanine increased lysine decarboxylase threefold over an equivalent weight of casein hydrolysate. The nine most stimulatory amino acids were methionine, arginine, cystine, leucine, isoleucine, glutamine, threonine, tyrosine, and asparagine. Methionine and arginine quantitatively were the most important. A mixture of these nine was 87% as effective as the complete mixture. Several amino acids were inhibitory at moderate concentrations, and alanine (2.53 mM) was the most effective. Added pyridoxine increased lysine decarboxylase activity 30%, whereas other B vitamins and cyclic adenosine 5'-monophosphate had no effect.
D000596:Amino Acids / Q000378:metabolism*; D002262:Carboxy-Lyases / Q000096:biosynthesis* / Q000378:metabolism; D002364:Caseins / Q000378:metabolism; D002474:Cell-Free System; D002854:Chromatography, Paper; D004790:Enzyme Induction; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism; D008239:Lysine; D010455:Peptides / Q000032:analysis / Q000378:metabolism*; D011736:Pyridoxine / Q000378:metabolism; D013053:Spectrophotometry
D000596:Amino Acids; D002364:Caseins; D010455:Peptides; D002262:Carboxy-Lyases; D008239:Lysine; D011736:Pyridoxine