Production and reactivity of immune sera specific for HADEN virus polypeptide antigens.
Antisera were prepared against the three structural polypeptides of HADEN virus dissociated with sodium dodecyl sulfate. These immunological reagents were used in immunofluorescence tests to study the kinetics and location of polypeptide antigen appearance in infected cells. These sera did not neutralize virus infectivity, did not cross-react with adenovirus-associated virus-infected cells, and reacted in complement fixation tests with sodium dodecyl sulfate-dissociated virus, but not with complete virus antigen. The polypeptide antigens were heat labile, and all appeared in infected cells at least 2 h prior to whole-virus antigen.
D000256:Adenoviridae / Q000276:immunology; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000917:Antibody Formation; D000956:Antigens, Viral; D002417:Cattle; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology; D003168:Complement Fixation Tests; D003429:Cross Reactions; D003593:Cytoplasm / Q000276:immunology; D004267:DNA Viruses / Q000032:analysis / Q000254:growth & development / Q000276:immunology*; D003720:Densitometry; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs / Q000276:immunology; D007106:Immune Sera; D007668:Kidney; D009500:Neutralization Tests; D010455:Peptides / Q000302:isolation & purification; D012526:Satellite Viruses / Q000276:immunology; D012967:Sodium Dodecyl Sulfate; D014764:Viral Proteins / Q000302:isolation & purification
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007106:Immune Sera; D010455:Peptides; D014764:Viral Proteins; D012967:Sodium Dodecyl Sulfate