Isolation of Chlamydia trachomatis by use of 5-iodo-2-deoxyuridine-treated cells.
Irradiated McCoy cells have provided a useful technique for the isolation of Chlamydia trachomatis strains, among which are found the etiological agents of trachoma, inclusion conjunctivitis, and lymphogranuloma venereum. Because irradiation is not always readily available, 5-iodo-2-deoxyuridine (IUDR) treatment of cells was investigated as a substitute procedure. IUDR-treated cells were found to be as sensitive to C. trachomatis infection as were irradiated McCoy cells. Stock chlamydial strains gave similar titers of iodine-stained inclusions in either system. When cells treated with IUDR were compared with irradiated cells for the isolation of C. trachomatis from clinical specimens, 5 of 138 specimens yielded isolates in IUDR-treated cells not found in irradiated ones, and one isolate was obtained from irradiated but not from IUDR-treated cells. In those 56 cases where inclusions were seen in both systems, there were significantly more inclusions in IUDR-treated than in irradiated cells. Although this series of cultures is too small to determine whether IUDR-treated cells are superior to irradiated ones for the isolation of C. trachomatis, the data indicate that IUDR treatment is at least equally effective.
D000818:Animals; D001431:Bacteriological Techniques; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002478:Cells, Cultured / Q000187:drug effects* / Q000528:radiation effects; D002584:Cervix Uteri / Q000382:microbiology; D002689:Chlamydia / Q000302:isolation & purification*; D003037:Cobalt Radioisotopes; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007065:Idoxuridine / Q000494:pharmacology*; D008297:Male; D008722:Methods; D051379:Mice; D011830:Radiation Effects; D014521:Urethra / Q000382:microbiology
D003037:Cobalt Radioisotopes; D001973:Bromodeoxyuridine; D007065:Idoxuridine