Release of IF2 from native ribosomes by dilution.
D002499:Centrifugation, Density Gradient; D004926:Escherichia coli / Q000166:cytology / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D010442:Peptide Chain Initiation, Translational; D010448:Peptide Initiation Factors; D012270:Ribosomes / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D014461:Ultracentrifugation
D010448:Peptide Initiation Factors