Immunofluorescent identification of mycoplasma colonies grown on agar-covered glass slides.
Mycoplasma colonies were grown on microscope slides overlaid with solid medium. After the slides were dried, washed, acetone fixed, and stained with fluorescein-conjugated antisera, specific immunofluorescence was easily observed against a dark background.
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D008722:Methods; D009174:Mycoplasma / Q000276:immunology / Q000302:isolation & purification*; D011817:Rabbits / Q000276:immunology
D000942:Antigens, Bacterial; D007106:Immune Sera