Non-continuous translation of coat protein and ribonucleic acid polymerase cistrons in MS2 bacteriophage ribonucleic acid.
In an MS2 phage ribonucleic acid (RNA)-directed in vitro protein-synthesizing system, the coat protein cistron and the adjacent RNA polymerase cistron are translated non-continuously. The ribosomes which have completed the synthesis of coat protein dissociate from the MS2 RNA and do not read through the intercistronic gap. Translation of the adjacent RNA polymerase cistron requires ribosomes other than those translating the coat protein cistron.
D000596:Amino Acids / Q000378:metabolism; D002474:Cell-Free System; D003090:Coliphages / Q000201:enzymology / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D005796:Genes; D010448:Peptide Initiation Factors; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D014316:Tritium; D014764:Viral Proteins / Q000096:biosynthesis*
D000596:Amino Acids; D010448:Peptide Initiation Factors; D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium; D012321:DNA-Directed RNA Polymerases