title: The nucleotide sequence of the lac operator.
abstract: The lac repressor protects the lac operator against digestion with deoxyribonuclease. The protected fragment is double-stranded and about 27 base-pairs long. We determined the sequence of RNA transcription copies of this fragment and present a sequence for 24 base pairs. It is: 5'--T G G A A T T G T G A G C G G A T A A C A A T T 3' 3'--A C C T T A A C A C T C G C C T A T T G T T A A 5' The sequence has 2-fold symmetry regions; the two longest are separated by one turn of the DNA double helix.
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000032:analysis* / Q000378:metabolism; D006868:Hydrolysis; D007785:Lactose / Q000378:metabolism*; D009711:Nucleotides / Q000032:analysis*; D009876:Operon; D010761:Phosphorus Radioisotopes; D011119:Polynucleotides / Q000032:analysis; D012260:Ribonucleases
chemical_list: D004269:DNA, Bacterial; D009711:Nucleotides; D010761:Phosphorus Radioisotopes; D011119:Polynucleotides; D012260:Ribonucleases; D007785:Lactose