In vitro repair of apurinic sites in DNA.
Apurinic sites disappear from DNA during an incubation of the DNA with the Escherichia coli endonuclease specific for apurinic sites, DNA polymerase I (EC 2.7.7.7.), and T4 ligase (EC 6.5.1.1). Omission of any one of these three enzymes and, in particular, omission of the endonuclease specific for apurinic sites prevents this in vitro repair.
D001665:Binding Sites; D003090:Coliphages / Q000378:metabolism*; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D004260:DNA Repair; D004267:DNA Viruses / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism; D004926:Escherichia coli; D009691:Nucleic Acid Denaturation; D011118:Polynucleotide Ligases / Q000378:metabolism
D004279:DNA, Viral; D004254:DNA Nucleotidyltransferases; D004720:Endonucleases; D011118:Polynucleotide Ligases