Production of spore spheroplasts of Clostridium botulinum and DNA extraction for density gradient centrifugation.
D002462:Cell Membrane; D002467:Cell Nucleus; D002473:Cell Wall / Q000187:drug effects; D002499:Centrifugation, Density Gradient; D003014:Clostridium botulinum / Q000166:cytology / Q000187:drug effects*; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D008722:Methods; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D008970:Molecular Weight; D009113:Muramidase / Q000494:pharmacology; D009995:Osmosis; D013056:Spectrophotometry, Ultraviolet; D013104:Spheroplasts / Q000302:isolation & purification*; D013171:Spores, Bacterial / Q000166:cytology / Q000187:drug effects*; D013395:Sucrose; D013936:Thymidine; D014316:Tritium
D004269:DNA, Bacterial; D014316:Tritium; D013395:Sucrose; D009113:Muramidase; D013936:Thymidine