Evaluation of frozen fixed smears for use in fluorescent antibody studies of salmonellae.
Smears of broth cultures of 28 Salmonella serotypes were fixed with Kirkpatrick fixative and stored at -20 C. Results indicate that organisms retain the ability to stain at maximal fluorescence intensity for as long as 2 years.
D000907:Antibodies, Bacterial / Q000302:isolation & purification*; D000942:Antigens, Bacterial; D005069:Evaluation Studies as Topic; D005189:False Positive Reactions; D005455:Fluorescent Antibody Technique; D005615:Freezing; D012475:Salmonella / Q000276:immunology*; D012703:Serotyping; D013997:Time Factors
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial