Comparative studies on functions of human adenovirus type 12 and its low oncogenic mutant virions.
Physical and biological properties of highly oncogenic human adenovirus type 12 were compared with a low oncogenic mutant (cyt mutant). Parental and cyt mutant virions had very similar density and DNA size. However, the parental strain virion preparations contained a much higher proportion of defective virions (capable of cell killing, but not able to induce T- or V-antigen) than cyt mutant stock. It was also found that cyt mutant had a reduced virus yield in several human cell lines compared with the parental strain.
D000256:Adenoviridae / Q000032:analysis / Q000254:growth & development / Q000276:immunology; D000327:Adsorption; D000956:Antigens, Viral / Q000032:analysis; D002250:Carbon Radioisotopes; D002277:Carcinoma; D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D004279:DNA, Viral / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006649:Histocompatibility Antigens / Q000032:analysis; D006801:Humans; D007668:Kidney / Q000196:embryology; D007822:Laryngeal Neoplasms; D009062:Mouth Neoplasms; D009154:Mutation; D009858:Oncogenic Viruses / Q000032:analysis / Q000254:growth & development / Q000276:immunology; D013936:Thymidine; D014316:Tritium; D014779:Virus Replication
D000956:Antigens, Viral; D002250:Carbon Radioisotopes; D004279:DNA, Viral; D006649:Histocompatibility Antigens; D014316:Tritium; D013936:Thymidine