Further characterization of staphylococcal gamma-hemolysin.
It was confirmed that staphylococcal gamma-hemolysin is composed of two separate proteins (gamma-lysin components I and II) which act synergistically. The molecular weights of the two components, determined by gel filtration, are 29,000 and 26,000, respectively, and their isoelectric points, determined by isoelectric focusing, are at pH 9.8 and 9.9. Both components are susceptible to the action of Pronase and subtilisin. A wide range of lipids, some in minute amounts, are capable of inhibiting the hemolytic activity of gamma-lysin.
D001431:Bacteriological Techniques; D003911:Dextrans / Q000494:pharmacology; D005227:Fatty Acids / Q000494:pharmacology; D006460:Hemolysin Proteins / Q000032:analysis* / Q000302:isolation & purification; D006461:Hemolysis / Q000187:drug effects; D006493:Heparin / Q000494:pharmacology; D007525:Isoelectric Focusing; D008055:Lipids / Q000494:pharmacology; D008970:Molecular Weight; D010743:Phospholipids / Q000494:pharmacology; D011402:Pronase / Q000378:metabolism; D013210:Staphylococcus / Q000378:metabolism*; D013381:Subtilisins / Q000378:metabolism; D014343:Trypan Blue / Q000494:pharmacology
D003911:Dextrans; D005227:Fatty Acids; D006460:Hemolysin Proteins; D008055:Lipids; D010743:Phospholipids; D006493:Heparin; D013381:Subtilisins; D011402:Pronase; D014343:Trypan Blue