Recipient gene duplication during generalized transduction.
An Hfr13 Delta(proA-lac) deletion recipient, -Delta(proA-lac)-F-purE(+)-, has been utilized in a study of the origins of duplications formed during chromosome fragment integration. Among the Pro(-)Lac(+) transductants, some have duplications spanning the F locus. These transductants are, or segregate, strains with F' episomes carrying genes of the duplication. Some of the duplications include purE(+), a gene which is not coinherited with lac(+) during bacteriophage P1-mediated transduction. Thus recipient genes have been duplicated during recombinant formation. Crossing-over models including replication steps provide a basis for explaining the duplication process.
D002869:Chromosome Aberrations; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003090:Coliphages; D003227:Conjugation, Genetic; D003434:Crossing Over, Genetic; D004926:Escherichia coli; D005111:Extrachromosomal Inheritance; D005796:Genes; D012328:RNA Viruses; D011995:Recombination, Genetic; D012723:Sex; D014161:Transduction, Genetic