Evaluation of the Minitek system for identification of Enterobacteriaceae.
Clinical isolates (869) and stock cultures (35) of Enterobacteriaceae were tested in parallel with the Minitek and conventional systems. The Minitek correctly identified 822 of 904 cultures. When a deoxyribonuclease plate was inoculated along with the Minitek, it was possible to speciate Enterobacteriaceae within 24 h. False-positive hydrogen sulfide reactions were the major fault with this system. Reactions were clear-cut and easy for technologists to read.
D001431:Bacteriological Techniques / Q000592:standards; D003851:Deoxyribonucleases / Q000096:biosynthesis; D004755:Enterobacteriaceae / Q000145:classification* / Q000201:enzymology / Q000378:metabolism; D005069:Evaluation Studies as Topic; D005188:False Negative Reactions; D005189:False Positive Reactions; D006862:Hydrogen Sulfide / Q000096:biosynthesis; D007211:Indoles / Q000096:biosynthesis; D014508:Urea / Q000378:metabolism
D007211:Indoles; D014508:Urea; D003851:Deoxyribonucleases; D006862:Hydrogen Sulfide