The distribution of blood-group antigens on butanol extraction of human erythrocyte 'ghosts'.
The distribution of protein and blood-group-antigen activity obtained after butanol extraction of erythrocyte ;ghosts' under various conditions is described. Butanol extraction under low-ionic strength conditions results in the recovery of membrane protein in high yield in the aqueous phase. Blood-group-A activity is found in both the aqueous and butanol phases, whereas blood-group-P activity is confined to the butanol phase and blood-group-I and blood-group-MN activity are restricted to the aqueous phase. Much lower yields of protein are obtained in the aqueous phase when high-ionic-strength conditions are used. An appreciable amount of material is precipitated at the interface. Under these conditions blood-group-P activity is found only in the butanol phase, blood group-A activity in the butanol phase and interface material and only blood-group-MN activity in the aqueous phase. In contrast with previous reports no correlation could be demonstrated between the secretor status of the donors and the presence of blood-group-A activity in the aqueous phase after butanol extraction under any of the extraction conditions used. By using butanol extraction under high-ionic-strength conditions it is possible to isolate the blood-group-MN-active sialoglycoprotein in high yield from erythrocyte ;ghosts' by a simple procedure.
D000017:ABO Blood-Group System / Q000032:analysis*; D000818:Animals; D000440:Butanols; D002462:Cell Membrane / Q000032:analysis / Q000276:immunology; D002498:Centrifugation; D002850:Chromatography, Gel; D003956:Dialysis; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000276:immunology*; D006023:Glycoproteins / Q000032:analysis; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D008858:Microscopy, Phase-Contrast; D009438:Neuraminic Acids / Q000032:analysis; D011817:Rabbits / Q000276:immunology
D000017:ABO Blood-Group System; D000440:Butanols; D006023:Glycoproteins; D009438:Neuraminic Acids