Penicillium stoloniferum virus: large-scale concentration and purification by polyethylene glycol.
An experimental procedure developed to concentrate fungal viruses from large volumes of homogenized mycelia with water-soluble polymers, such as polyethylene glycol, possesses advantages over more conventional methods. Concentration of the Penicillium stoloniferum fast-moving virus from mycelial homogenates after addition of polyethylene glycol was rapid and produced large quantities of pure virus.
D000645:Ammonium Sulfate; D002458:Cell Fractionation; D011232:Chemical Precipitation; D004591:Electrophoresis, Polyacrylamide Gel; D010407:Penicillium; D010942:Plant Viruses / Q000302:isolation & purification*; D011092:Polyethylene Glycols; D012313:RNA; D012328:RNA Viruses; D012441:Saccharomyces cerevisiae; D012965:Sodium Chloride; D014461:Ultracentrifugation
D011092:Polyethylene Glycols; D012965:Sodium Chloride; D012313:RNA; D000645:Ammonium Sulfate