title: Genetic analysis of the active sites of lac repressor.
abstract: Selection methods for the isolation of i(-d) and i(s) mutants are described. Two hundred and forty-seven i(-d) and 98 i(s) mutations have been localized by deletion mapping. All i(-d) mutations map in the region of the i gene, which codes for the aminoterminal part of the lac repressor, whereas i(s) mutations map in the middle of the i gene and at the proximal end of the i(-d) cluster.
mesh_terms: D001665:Binding Sites; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000378:metabolism; D004790:Enzyme Induction; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism; D005696:Galactosidases / Q000378:metabolism; D005809:Genes, Regulator; D009154:Mutation; D009876:Operon; D011485:Protein Binding
chemical_list: D004269:DNA, Bacterial; D005696:Galactosidases