title: a simple device for obtaining complementary fracutre planes at liquid helium temperature in the freeze-etching technique.
mesh_terms: D002462:Cell Membrane / Q000648:ultrastructure; D002473:Cell Wall / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D005613:Freeze Etching / Q000295:instrumentation* / Q000379:methods; D006371:Helium; D008854:Microscopy, Electron; D012441:Saccharomyces cerevisiae / Q000648:ultrastructure*; D014617:Vacuoles / Q000648:ultrastructure
chemical_list: D006371:Helium