title: Replication of T4 DNA in Escherichia coli treated with toluene.
abstract: Deoxyribonucleoside triphosphates are incorporated into T4 DNA in infected cells treated with toluene. Under the proper conditions the incorporation is controlled by the known T4 DNA polymerase and proceeds by a semiconservative mechanism. Both strands of the phage DNA are replicated into a high molecular weight progeny molecule. The replication system is accessible to extracellular pancreatic DNase added to the reaction mixture. At early times after infection a second replication system, not under control of the gene 43 polymerase, has been detected which synthesizes T4 DNA in toluenized cells.
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D001973:Bromodeoxyuridine / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D002845:Chromatography; D003090:Coliphages / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D003851:Deoxyribonucleases; D003854:Deoxyribonucleotides / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects*; D006882:Hydroxyapatites; D008274:Magnesium / Q000494:pharmacology; D008970:Molecular Weight; D010636:Phenols; D010761:Phosphorus Radioisotopes; D011402:Pronase; D014050:Toluene / Q000494:pharmacology*; D014316:Tritium; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D003854:Deoxyribonucleotides; D006882:Hydroxyapatites; D010636:Phenols; D010761:Phosphorus Radioisotopes; D014316:Tritium; D014050:Toluene; D000255:Adenosine Triphosphate; D004254:DNA Nucleotidyltransferases; D003851:Deoxyribonucleases; D011402:Pronase; D001973:Bromodeoxyuridine; D008274:Magnesium