Penetration of somatic mammalian cells by sperm.
Penetration of somatic mammalian cells by spermatozoa occurred after simple admixture in culture. With sperm labeled in vivo, autoradiography revealed incorporation of DNA into nuclei of recipient cells, indicating release of DNA after entrance by sperm. This system provides a new approach to study the molecular biology of information transfer and of haploid gene expression.
D000818:Animals; D001345:Autoradiography; D002460:Cell Line; D002478:Cells, Cultured; D006224:Cricetinae; D004247:DNA / Q000096:biosynthesis / Q000378:metabolism*; D005347:Fibroblasts; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D014176:Protein Biosynthesis; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D013936:Thymidine / Q000378:metabolism; D014158:Transcription, Genetic; D014316:Tritium
D014316:Tritium; D004247:DNA; D013936:Thymidine