Limitations of the use of 5-fluorouracil as a selective agent for the isolation of leptospirae.
It has been shown that some component of Fletcher medium is able to annul the inhibitory action of 5-fluorouracil (400 mug/ml) on bacteria other than leptospirae. The most likely ingredient which can be implicated in this context appears to be beef extract.
D000332:Aerobiosis; D000818:Animals; D001419:Bacteria / Q000254:growth & development; D001431:Bacteriological Techniques; D002417:Cattle; D003470:Culture Media; D004352:Drug Resistance, Microbial; D004925:Escherichia / Q000254:growth & development; D005472:Fluorouracil / Q000494:pharmacology*; D007106:Immune Sera; D007919:Leptospira / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D008460:Meat; D008837:Micrococcus / Q000254:growth & development; D010461:Peptones; D012756:Sheep / Q000276:immunology; D013210:Staphylococcus / Q000254:growth & development; D013696:Temperature; D014020:Tissue Extracts
D003470:Culture Media; D007106:Immune Sera; D010461:Peptones; D014020:Tissue Extracts; D005472:Fluorouracil