Residual thymidine incorporation in the presence of chloramphenicol in synchronous cultures of Escherichia coli B-r.
Escherichia coli B/r ATCC 12407 which grew synchronously with interdivision times of 45 and 85 min were exposed at intervals to chloramphenicol (200 mug/ml) and [(14)C]thymidine for short or long (residual) periods. The results suggested that the pattern of residual thymidine incorporation in 85-min cells yielded an estimate of the length of the D period rather than the time of initiation of chromosome replication.
D002250:Carbon Radioisotopes; D002455:Cell Division; D002701:Chloramphenicol / Q000494:pharmacology*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D013386:Succinates / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism*; D013997:Time Factors
D002250:Carbon Radioisotopes; D004269:DNA, Bacterial; D013386:Succinates; D002701:Chloramphenicol; D005947:Glucose; D013936:Thymidine