Neutralizing antibody response of guinea pigs to lymphocytic choriomeningitis virus.
A 24-h neutralization test that is based on fluorescent cell counting was used for the detection and quantitative determination of serum-neutralizing antibody against lymphocytic choriomeningitis virus. The earliest manifestation of serum-neutralizing antibody in guinea pigs was shown to occur within 1 week after inoculation with lymphocytic choriomeningitis virus. Within 11 weeks, serum-neutralizing antibody increased from 20- to 300-fold. Neutralizing end points of serum samples, obtained from 3 through 11 weeks, were enhanced as much as sevenfold by complement. Anti-guinea pig immunoglobulin G or anti-whole guinea pig serum potentiated the neutralizing activity of serum as much as 20- and 40-fold, respectively.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral / Q000032:analysis*; D000917:Antibody Formation; D003165:Complement System Proteins; D006224:Cricetinae; D046508:Culture Techniques; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs / Q000276:immunology; D007106:Immune Sera; D007074:Immunoglobulin G; D007668:Kidney; D008216:Lymphocytic Choriomeningitis / Q000276:immunology*; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology*; D051379:Mice; D009500:Neutralization Tests; D014776:Virus Cultivation
D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral; D007106:Immune Sera; D007074:Immunoglobulin G; D003165:Complement System Proteins