Regulation of one-carbon biosynthesis and utilization in Escherichia coli.
The regulation of several enzymes involved in one-carbon metabolism was studied in a mutant of Escherichia coli K-12 defective in S-adenosylmethionine synthetase. The mutant that was reported to have a low endogenous concentration of S-adenosylmethionine had elevated levels of N-5, 10-methylene tetrahydrofolate reductase and serine transhydroxymethylase, but the level of N-5, 10-methylene tetrahydrofolate dehydrogenase was not altered. These results suggest that S-adenosylmethionine plays a role in the regulation of one-carbon production and utilization. An enzyme system that cleaved glycine to one-carbon units was demonstrated. The enzymes responsible for the cleavage of glycine were induced by exogenous glycine but were independent of S-adenosylmethionine or purine levels in the cell.
D000225:Adenine / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D002250:Carbon Radioisotopes; D002474:Cell-Free System; D003470:Culture Media; D004794:Enzyme Repression; D004926:Escherichia coli / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D005557:Formaldehyde / Q000096:biosynthesis*; D005998:Glycine / Q000378:metabolism*; D008025:Ligases / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D009154:Mutation; D009705:Nucleosides; D010088:Oxidoreductases / Q000378:metabolism; D012436:S-Adenosylmethionine / Q000378:metabolism; D012694:Serine / Q000378:metabolism; D013095:Spermidine / Q000378:metabolism; D013763:Tetrahydrofolates; D014166:Transferases / Q000378:metabolism
D002250:Carbon Radioisotopes; D003470:Culture Media; D009705:Nucleosides; D013763:Tetrahydrofolates; D005557:Formaldehyde; D012694:Serine; D012436:S-Adenosylmethionine; D008715:Methionine; D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; D014166:Transferases; D008025:Ligases; D000225:Adenine; D005998:Glycine; D013095:Spermidine