Induction of guinea pig B-cell lymphokine synthesis by mitogenic and nonmitogenic signals to Fc, Ig, and C3 receptors.
This study shows that bone marrow-derived lymphocytes of guinea pigs if appropriately activated produce a monocyte chemotactic factor (MNL CTX). Activation of B lymphocytes to produce a chemotactic lymphokine occurs subsequent to interactions with a variety of membrane-associated receptors. Polymeric B-cell mitogens with multiple binding sites, polymerized flagellin and lipopolysaccharide, initiated mediator synthesis. Furthermore, interaction of antigen-antibody complexes or aggregated gamma globulin with the Fc receptor and binding of antigen-antibody-complement complexes at the C3 receptor can effectively facilitate mediator production in the absence of a significant proliferative response. Additionally, intact anti-immunoglobulin but not its Fab fragments activated the B cells. An anti-Fab effectively converted the inactive Fab-bound B cells into producers of MNL CTX, suggesting that the basic mechanism of activation depended upon cross-linking of receptors. Thus, interaction of B-cell surface receptors such as Fc, Ig, and C3 sites with mitogenic as well as nonmitogenic molecules capable of bridging the receptors appears to trigger B-cell mediator production.
D000818:Animals; D000941:Antigens; D000942:Antigens, Bacterial; D001402:B-Lymphocytes / Q000276:immunology*; D001666:Binding Sites, Antibody; D002469:Cell Separation; D003165:Complement System Proteins; D003208:Concanavalin A; D005407:Flagella / Q000276:immunology; D006168:Guinea Pigs; D007141:Immunoglobulin Fc Fragments; D007136:Immunoglobulins; D037102:Lectins; D008070:Lipopolysaccharides / Q000276:immunology; D008213:Lymphocyte Activation; D008263:Macrophage Migration-Inhibitory Factors / Q000096:biosynthesis*; D008934:Mitogens; D009578:Nitrobenzenes; D010047:Ovalbumin / Q000276:immunology; D011135:Polysaccharides, Bacterial; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D000941:Antigens; D000942:Antigens, Bacterial; D007141:Immunoglobulin Fc Fragments; D007136:Immunoglobulins; D037102:Lectins; D008070:Lipopolysaccharides; D008263:Macrophage Migration-Inhibitory Factors; D008934:Mitogens; D009578:Nitrobenzenes; D011135:Polysaccharides, Bacterial; D014316:Tritium; D003208:Concanavalin A; D010047:Ovalbumin; D003165:Complement System Proteins; D013936:Thymidine