Replication and transcription of eukaryotic DNA in Escherichia coli.
Fragments of amplified Xenopus laevis DNA, coding for 18S and 28S ribosomal RNA and generated by EcoRI restriction endonuclease, have been linked in vitro to the bacterial plasmid pSC101; and the recombinant molecular species have been introduced into E. coli by transformation. These recombinant plasmids, containing both eukaryotic and prokaryotic DNA, replicate stably in E. coli. RNA isolated from E. coli minicells harboring the plasmids hybridizes to amplified X. laevis rDNA.
D000818:Animals; D004247:DNA / Q000032:analysis; D004261:DNA Replication; D004352:Drug Resistance, Microbial; D004586:Electrophoresis; D004926:Escherichia coli / Q000378:metabolism*; D005111:Extrachromosomal Inheritance; D008854:Microscopy, Electron; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010761:Phosphorus Radioisotopes; D012329:RNA, Bacterial; D011995:Recombination, Genetic; D013752:Tetracycline; D014158:Transcription, Genetic; D014170:Transformation, Genetic; D014316:Tritium; D014461:Ultracentrifugation; D014981:Xenopus
D010761:Phosphorus Radioisotopes; D012329:RNA, Bacterial; D014316:Tritium; D004247:DNA; D013752:Tetracycline