title: Protein synthesis in a cell-free system prepared from human placenta. II. pH 5 enzyme inefficiency due to defects in tRNA charging with resulting loss of elongation factor 1.
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D002499:Centrifugation, Density Gradient; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004926:Escherichia coli; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007930:Leucine; D008099:Liver / Q000201:enzymology; D010445:Peptide Elongation Factors; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000378:metabolism; D012335:RNA, Ribosomal / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D051381:Rats; D012270:Ribosomes / Q000201:enzymology*; D013307:Streptomycin; D013997:Time Factors; D046249:Transfer RNA Aminoacylation; D014316:Tritium
chemical_list: D002250:Carbon Radioisotopes; D010445:Peptide Elongation Factors; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D014316:Tritium; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases; D007930:Leucine; D013307:Streptomycin