title: The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
abstract: With a stepwise degradation and terminal labeling procedure the 3'-terminal sequence of E. coli 16S ribosomal RNA is shown to be Pyd-A-C-C-U-C-C-U-U-A(OH). It is suggested that this region of the RNA is able to interact with mRNA and that the 3'-terminal U-U-A(OH) is involved in the termination of protein synthesis through base-pairing with terminator codons. The sequence A-C-C-U-C-C could recognize a conserved sequence found in the ribosome binding sites of various coliphage mRNAs; it may thus be involved in the formation of the mRNA.30S subunit complex.
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis; D001483:Base Sequence; D001665:Binding Sites; D002852:Chromatography, Ion Exchange; D003090:Coliphages / Q000378:metabolism; D004590:Electrophoresis, Paper; D004926:Escherichia coli / Q000032:analysis*; D005815:Genetic Code; D006868:Hydrolysis; D009154:Mutation; D009841:Oligonucleotides / Q000032:analysis*; D010442:Peptide Chain Initiation, Translational; D010443:Peptide Chain Termination, Translational; D012329:RNA, Bacterial / Q000032:analysis* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012335:RNA, Ribosomal / Q000032:analysis* / Q000378:metabolism; D012260:Ribonucleases; D012270:Ribosomes / Q000378:metabolism; D014316:Tritium; D014764:Viral Proteins / Q000096:biosynthesis
chemical_list: D001426:Bacterial Proteins; D009841:Oligonucleotides; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D014764:Viral Proteins; D014316:Tritium; D012260:Ribonucleases