title: Con--mutants: class of mutants in Escherichia coli K-12 lacking a major cell wall protein and defective in conjugation and adsorption of a bacteriophage.
abstract: We describe a new class of mutants of Escherichia coli K-12 defective in conjugation (Con(-) mutants). They lack a major protein of the outer membrane and are defective as recipients with Hfr and F' donors and as recipients for several F-like R factors and Col factors. In the case of crosses with an Hfr donor, we have shown that the Con(-) recipient is defective in pair formation. The mutants are resistant to certain phages due to loss of receptor activity.
mesh_terms: D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000032:analysis; D002473:Cell Wall / Q000032:analysis; D003090:Coliphages / Q000254:growth & development; D003227:Conjugation, Genetic; D004352:Drug Resistance, Microbial; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000032:analysis / Q000187:drug effects; D008242:Lysogeny; D009154:Mutation; D012328:RNA Viruses; D011995:Recombination, Genetic
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins