Beta-galactosidase from termination and deletion mutant strains.
beta-Galactosidase fragments were isolated from strains of Escherichia coli with mutations in the lacZ gene. The polypeptide obtained from a termination mutant (lacZNG125) appeared to be the intact gene product, containing the first half of the beta-galactosidase amino acid sequence. From an internal deletion mutant strain (lacZU163), an aggregate was obtained of several partially degraded polypeptides. Each of these was smaller than predicted from genetic data for the fragment. Introduction of the lacZU163 mutation into a protein degradation-deficient strain (Deg(-)) resulted in the protection of the amino-terminal region of the protein. Some of the BrCN peptides from the U163 polypeptides were separated and identified. From such experiments it was shown that in both Deg(-) and Deg(+) strains the COOH-terminal region is rapidly degraded. This indicates that the complete gene product of lacZU163 has not been detected. The use of genetically defined enzyme fragments in studying structure-function relationships and in determination of primary structure is discussed.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D011232:Chemical Precipitation; D002846:Chromatography, Affinity; D002874:Chromosome Mapping; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D005696:Galactosidases / Q000032:analysis* / Q000302:isolation & purification; D005796:Genes; D005816:Genetic Complementation Test; D007202:Indicators and Reagents; D009154:Mutation; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification
D000596:Amino Acids; D007202:Indicators and Reagents; D010455:Peptides; D005696:Galactosidases; D003488:Cyanogen Bromide