Replacement of ribosomal protein S1 by interference factor ialpha in ribosomal binding of phage Ms2 RNA.
The MS2 RNA binding capacity of 30S ribosomal subunits, which is lost when protein S1 is removed, can be restored following incubation with interference factor ialpha and repelleting. Polyacrylamide-agarose gel electrophoresis shows that, under these conditions, a faster moving, non-RNA binding 30S species, which contains no S1, is converted to a slower moving RNA-binding one, having the same mobility as the 30S species that contains protein S1. Factor ialpha binds to single-stranded RNAs in a pattern that closely resembles the RNA binding pattern of initiation factor IF-3.
D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002250:Carbon Radioisotopes; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism* / Q000648:ultrastructure; D010442:Peptide Chain Initiation, Translational; D011072:Poly U / Q000378:metabolism; D011119:Polynucleotides / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D014316:Tritium; D014764:Viral Proteins / Q000096:biosynthesis
D001426:Bacterial Proteins; D002250:Carbon Radioisotopes; D011119:Polynucleotides; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium; D011072:Poly U