New method for large-scale preparation of covalently closed lambda DNA molecules.
A combination of mutations in bacteriophage lambda and its host Escherichia coli K-12 provides a convenient system for the isolation of large quantities of covalently closed circular DNA molecules. We describe two procedures for the large scale preparation of lambda DNA in the duplex circular form.
D001433:Bacteriolysis; D002499:Centrifugation, Density Gradient / Q000379:methods; D003090:Coliphages / Q000032:analysis*; D004261:DNA Replication; D004267:DNA Viruses; D004270:DNA, Circular / Q000302:isolation & purification*; D004279:DNA, Viral / Q000096:biosynthesis / Q000302:isolation & purification*; D004926:Escherichia coli; D004996:Ethidium; D008242:Lysogeny; D009154:Mutation; D010761:Phosphorus Radioisotopes; D011995:Recombination, Genetic; D013941:Thymine; D014316:Tritium
D004270:DNA, Circular; D004279:DNA, Viral; D010761:Phosphorus Radioisotopes; D014316:Tritium; D004996:Ethidium; D013941:Thymine