Effect of ribosomal wash factors on inhibition by chloramphenicol.
In vitro protein-synthesizing systems from Escherichia coli can be categorized as either chloramphenicol-sensitive or chloramphenicol-insensitive. The chloramphenicol-sensitive systems used in this study required the presence of factors removed from ribosomes with 1.0 m NH(4)Cl when chromatographically purified ribosomes were used for amino acid incorporation. These ribosomal wash factors inhibited but did not eliminate amino acid incorporation in chloramphenicol-insensitive systems. For both systems, addition of increasing amounts of the ribosomal wash factors increased the sensitivity to chloramphenicol inhibition.
D000596:Amino Acids / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis; D002701:Chloramphenicol / Q000494:pharmacology*; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D012270:Ribosomes
D000596:Amino Acids; D001426:Bacterial Proteins; D002701:Chloramphenicol