Intracellular immunoglobulins. A comparative study on three standard tissue processing methods using horseradish peroxidase and fluorochrome conjugates.
Formalin fixation followed by routine paraffin embedding allows the demonstration of intracellular immunoglobulin by a sandwich technique using either peroxidase or fluorescein isothyocyanate labelled antibody conjugates. The results compared favourably with those obtained using either the fresh frozen or the Sainte-Marie method for preserving tissue immunoglobulins. The formalin-paraffin method with peroxidase-labelled antibody has advantages for routine use. Morphology is excellent, preparations are permanent, and retrospective studies of stored paraffin-embedded tissue are possible. Some of the problems of labelled antibody studies are discussed.
D000818:Animals; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique / Q000379:methods; D005557:Formaldehyde; D006652:Histological Techniques; D006689:Hodgkin Disease / Q000473:pathology; D006801:Humans; D007106:Immune Sera; D007075:Immunoglobulin M; D007136:Immunoglobulins / Q000032:analysis*; D008198:Lymph Nodes / Q000276:immunology; D010232:Paraffin; D010544:Peroxidases; D011817:Rabbits / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013552:Swine / Q000276:immunology; D013861:Thiocyanates
D005452:Fluoresceins; D007106:Immune Sera; D007075:Immunoglobulin M; D007136:Immunoglobulins; D013861:Thiocyanates; D005557:Formaldehyde; D010232:Paraffin; D010544:Peroxidases