Properties of the relaxation complexes of supercoiled deoxyribonucleic acid and protein of the R plasmids R64, R28K, and R6K.
The presence of supercoiled deoxyribonucleic acid in the form of a relaxation complex is described for the antibiotic resistance plasmids R64, R28K, and R6K. The properties of these relaxation complexes indicate that they consist of a covalently closed circular deoxyribonucleic acid molecule associated with an activable, single strand-specific endonuclease.
D000667:Ampicillin / Q000494:pharmacology; D001426:Bacterial Proteins / Q000032:analysis*; D002250:Carbon Radioisotopes; D002499:Centrifugation, Density Gradient; D004269:DNA, Bacterial / Q000032:analysis*; D004270:DNA, Circular / Q000032:analysis*; D004720:Endonucleases / Q000032:analysis; D004926:Escherichia coli / Q000032:analysis* / Q000187:drug effects; D004996:Ethidium; D005111:Extrachromosomal Inheritance; D006358:Hot Temperature; D010403:Penicillin Resistance; D011402:Pronase; D012967:Sodium Dodecyl Sulfate; D013307:Streptomycin / Q000494:pharmacology; D013752:Tetracycline / Q000494:pharmacology; D013941:Thymine; D014316:Tritium
D001426:Bacterial Proteins; D002250:Carbon Radioisotopes; D004269:DNA, Bacterial; D004270:DNA, Circular; D014316:Tritium; D012967:Sodium Dodecyl Sulfate; D000667:Ampicillin; D004720:Endonucleases; D011402:Pronase; D004996:Ethidium; D013752:Tetracycline; D013941:Thymine; D013307:Streptomycin