Antiserum inactivation of electrophoretically purified M13 diploid virions: model for the F-specific filamentous bacteriophages.
Antiserum inactivation experiments were carried out on electrophoretically purified diploid virions from a cross between two complementing amber mutants of phage M13. The total (homozygous plus heterozygous) diploid population, assayed on a permissive host where only one genome is needed for plaque formation, was inactivated at the same rate as haploids. Heterozygous diploids, assayed on a nonpermissive host, where both genomes are needed for plaque formation, were twice as sensitive as haploids and the total diploid population. These results have led us to propose a model for serum inactivation of the F-specific filamentous phages. According to this model, phage-neutralizing antibodies attach anywhere along the length of the phage and allow the phage to penetrate only up to the first bound antibody molecule.
D000327:Adsorption; D000914:Antibodies, Viral; D001666:Binding Sites, Antibody; D003090:Coliphages / Q000254:growth & development* / Q000276:immunology / Q000302:isolation & purification; D003433:Crosses, Genetic; D004279:DNA, Viral; D004171:Diploidy; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D006238:Haploidy; D006579:Heterozygote; D007106:Immune Sera / Q000494:pharmacology*; D008954:Models, Biological; D009154:Mutation
D000914:Antibodies, Viral; D004279:DNA, Viral; D007106:Immune Sera