title: Calcium hydroxyapatite fractionation as a second ion exchange step in protein purification: improvement of flow rate and resolution.
mesh_terms: D000429:Alcohol Oxidoreductases / Q000302:isolation & purification; D000619:Aminohydrolases / Q000302:isolation & purification; D001215:Asparaginase / Q000302:isolation & purification; D002118:Calcium; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D003562:Cytidine; D004925:Escherichia / Q000201:enzymology; D005069:Evaluation Studies as Topic; D006882:Hydroxyapatites; D007709:Klebsiella / Q000201:enzymology; D008722:Methods; D011506:Proteins / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
chemical_list: D006882:Hydroxyapatites; D011506:Proteins; D003562:Cytidine; D000429:Alcohol Oxidoreductases; D001215:Asparaginase; D000619:Aminohydrolases; D002118:Calcium