Electron microscope analysis of partial denaturation of F factor deoxyribonucleic acid.
Partial denaturation pattern of sex factor deoxyribonucleic acid of Escherichia coli was studied by electron microscopy. Clustering of the adenine-plusthymine-rich regions in one part of the molecule was revealed. The positions of these regions were located on the physical map of F by analyzing the partial denaturation pattern of heteroduplexes between F and F-prime factors with various parts of F sequences deleted.
D000225:Adenine / Q000032:analysis; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis*; D004270:DNA, Circular / Q000032:analysis*; D004277:DNA, Single-Stranded / Q000032:analysis; D004926:Escherichia coli / Q000032:analysis*; D005111:Extrachromosomal Inheritance; D005559:Formamides; D008854:Microscopy, Electron; D008956:Models, Chemical; D009691:Nucleic Acid Denaturation; D012723:Sex; D013941:Thymine / Q000032:analysis
D004269:DNA, Bacterial; D004270:DNA, Circular; D004277:DNA, Single-Stranded; D005559:Formamides; D000225:Adenine; D013941:Thymine