title: Studies on in vitro DNA synthesis: purification of dna C gene product containing dna D activity from Escherichia coli.
abstract: The conversion of varphiX174 single-stranded DNA to duplex DNA by extracts of E. coli requires products of the E. coli DNA replication genes. By use of this complementation system, the dna C gene product has been purified from wild-type E. coli as well as from a dna C temperature-sensitive mutant. The latter preparations are temperature sensitive when compared to the wild-type gene product. The dna C and dna D gene products copurify, have similar characteristics, are both temperature sensitive in preparations from dna C temperature-sensitive cells, and are both undetectable in preparations from dna D temperature-sensitive cells.
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000302:isolation & purification* / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D002848:Chromatography, DEAE-Cellulose; D003090:Coliphages; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004279:DNA, Viral; D004926:Escherichia coli / Q000032:analysis / Q000378:metabolism*; D005796:Genes; D008970:Molecular Weight; D009711:Nucleotides / Q000378:metabolism; D013698:Templates, Genetic; D013942:Thymine Nucleotides / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004279:DNA, Viral; D009711:Nucleotides; D013942:Thymine Nucleotides