Rapid gentamicin bioassay using a multiple-antibiotic-resistant strain of Klebsiella pneumoniae.
A rapid bioassay for gentamicin levels in serum was developed by using a strain of Klebsiella pneumoniae that has multiple resistance to antibiotics. Assays were comparable when performed on either small or large petri plates, and results were available in 2 to 4 h. Studies showed an overall recovery of 97.6% for gentamicin alone or 104.5% in the presence of commonly used antibiotics. The procedure can be performed without the need to inactivate other antibiotics that may be present in the serum sample.
D001681:Biological Assay; D004352:Drug Resistance, Microbial; D005839:Gentamicins / Q000097:blood* / Q000494:pharmacology; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects*; D008722:Methods
D005839:Gentamicins