A method of isolating anucleated yeast protoplasts unable to synthesize the glucan fibrillar component of the wall.
D002467:Cell Nucleus; D002473:Cell Wall / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D003584:Cytological Techniques; D005613:Freeze Etching; D005947:Glucose; D008854:Microscopy, Electron; D011134:Polysaccharides / Q000096:biosynthesis*; D011523:Protoplasts / Q000187:drug effects / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000378:metabolism
D011134:Polysaccharides; D003513:Cycloheximide; D005947:Glucose