Effect of tetanolysin on platelets and lysosomes.
Tetanolysin, partially purified, caused the lysis of human and rabbit platelets, as determined by a decrease in the optical density of platelet suspensions and the release of serotonin, enzymes, and protein. This lytic activity was neutralized by antitoxin. In addition, a suspension of the lysosome-containing large granule fraction of rabbit liver released hydrolytic enzymes when exposed to tetanolysin. Thus, tetanolysin can be added to the list of bacterial toxins that are lytic for a variety of cellular or subcellular membranes. These findings provide additional data that suggest that tetanolysin may contribute to the pathogenesis of some of the unusual manifestations observed in clinical tetanus.
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D001792:Blood Platelets / Q000187:drug effects*; D002845:Chromatography; D003017:Clostridium tetani / Q000276:immunology; D003911:Dextrans; D005470:Fluorometry; D005966:Glucuronidase / Q000378:metabolism; D006460:Hemolysin Proteins / Q000032:analysis / Q000494:pharmacology*; D006801:Humans; D008099:Liver / Q000166:cytology; D008247:Lysosomes / Q000187:drug effects*; D008297:Male; D011817:Rabbits; D012701:Serotonin / Q000032:analysis; D013053:Spectrophotometry; D013743:Tetanus Antitoxin / Q000494:pharmacology
D001426:Bacterial Proteins; D003911:Dextrans; D006460:Hemolysin Proteins; D013743:Tetanus Antitoxin; D012701:Serotonin; D000135:Acid Phosphatase; D005966:Glucuronidase