title: Kinetics of genetic recombination in Escheria coli K-12: competition between genetic integration and degradation.
abstract: The effect of shifting zygotes from a rich medium into a poor medium (shift-down) on the frequency of recombination has been examined; our results suggest that protein syntheses other than those required for growth may be involved. Experiments with chloramphenicol support this notion, and further suggest that there is a competition between genetic integration and degradation in the recipient cells. By inhibiting protein synthesis one can shift the competition in favor of either integration or segregation, depending on the physiological state of the zygotes. By calculating the time required for segregation as one generation, we conclude that the recombination chromosome is homozygous at the time of integration.
mesh_terms: D000596:Amino Acids; D002452:Cell Count; D002455:Cell Division; D002701:Chloramphenicol / Q000494:pharmacology; D002876:Chromosomes, Bacterial; D003470:Culture Media; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D005947:Glucose; D006720:Homozygote; D007785:Lactose / Q000378:metabolism; D009154:Mutation; D011995:Recombination, Genetic; D013307:Streptomycin; D013386:Succinates; D013997:Time Factors
chemical_list: D000596:Amino Acids; D003470:Culture Media; D013386:Succinates; D002701:Chloramphenicol; D005947:Glucose; D007785:Lactose; D013307:Streptomycin