Characterization of aminoacyl transfer ribonucleic acid formation stimulated by polyamines.
From a comparative study of aminoacyl transfer ribonucleic acid (tRNA) formation stimulated by polyamines or by Mg(2+), it is shown that both reactions have the same requirements for adenosine triphosphate, tRNA, and aminoacyl-tRNA synthetase. Reaction kinetics are similar in both reactions. The same tRNA is aminoacylated in the presence of either spermine of Mg(2+). Aminoacyl-tRNA formed in the presence of polyamines can be a donor of amino acids in polypeptide synthesis.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000596:Amino Acids; D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification / Q000378:metabolism; D002250:Carbon Radioisotopes; D002474:Cell-Free System; D002852:Chromatography, Ion Exchange; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D007532:Isoleucine / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D010452:Peptide Biosynthesis; D010649:Phenylalanine / Q000378:metabolism; D011485:Protein Binding; D011700:Putrescine / Q000494:pharmacology*; D012329:RNA, Bacterial / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D013095:Spermidine / Q000494:pharmacology*; D013096:Spermine / Q000494:pharmacology*
D000596:Amino Acids; D002250:Carbon Radioisotopes; D012329:RNA, Bacterial; D007532:Isoleucine; D013096:Spermine; D010649:Phenylalanine; D000255:Adenosine Triphosphate; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases; D008274:Magnesium; D013095:Spermidine; D011700:Putrescine