Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.
A modified transformation procedure that is effective for the introduction of plasmid deoxyribonucleic acid at high frequency into Salmonella typhimurium, as well as into Escherichia coli, is described. Transformed bacteria acquire a circular deoxyribonucleic acid species having the genetic and molecular characteristics of the transforming plasmid.
D002499:Centrifugation, Density Gradient; D004269:DNA, Bacterial; D004270:DNA, Circular; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects; D005111:Extrachromosomal Inheritance; D008722:Methods; D009268:Nalidixic Acid / Q000494:pharmacology; D012486:Salmonella typhimurium / Q000187:drug effects*; D013752:Tetracycline / Q000494:pharmacology; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D004270:DNA, Circular; D009268:Nalidixic Acid; D013752:Tetracycline