A continuous spectrophotometric assay for the transacylase (E2) component of pyruvate and alpha-oxoglutarate dehydrogenase enzyme complexes.
D000150:Acinetobacter / Q000201:enzymology; D000217:Acyltransferases / Q000032:analysis*; D008058:Dihydrolipoamide Dehydrogenase; D004926:Escherichia coli / Q000201:enzymology; D007655:Ketoglutarate Dehydrogenase Complex / Q000032:analysis*; D007700:Kinetics; D008722:Methods; D009097:Multienzyme Complexes / Q000032:analysis*; D011768:Pyruvate Dehydrogenase Complex / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
D009097:Multienzyme Complexes; D011768:Pyruvate Dehydrogenase Complex; D007655:Ketoglutarate Dehydrogenase Complex; D008058:Dihydrolipoamide Dehydrogenase; D000217:Acyltransferases