A central vasodepressor effect of Dyflos.
1 Cats were anaesthetized with pentobarbitone sodium and atropinized peripherally by intravenous injection of atropine methyl nitrate; the effect was examined of topical bilateral application of dyflos to the ventral surface of the medulla oblongata at a region lateral to the pyramids and caudal to the trapezoid bodies. Dyflos was applied by means of perspex rings; the volume of fluid placed in each ring was 10 mul.2 The topical application of dyflos (1-20 mg/ml) produced a fall in arterial blood pressure without changes in heart rate and, in experiments without artificial ventilation, tachypnoea with dissociation of thoracic and abdominal respiration.3 Atropine methyl nitrate (50 mg/ml) applied topically in the same way as dyflos, prevented or abolished its vasodepressor effect.4 The two reactivators of acetylcholinesterase, obidoxime (100-200 mg/ml) and pralidoxime mesylate (100-200 mg/ml), applied topically in the same way as dyflos, abolished its vasodepressor effect. The reactivator compound 30 (100 mg/ml), also a pyridinium aldoxime, did not have this effect.5 Obidoxime and pralidoxime mesylate also reversed the vasodepression produced by carbachol applied to the ventral surface of the brain stem but not the vasodepression produced by glycine similarly applied.6 The problem is discussed as to whether the reversal of the dyflos and carbachol-induced vasodepression by obidoxime and pralidoxime is due to acetylcholinesterase reactivation by dephosphorylation and decarbamylation respectively, to a central atropine-like action of these compounds or to a combination of both.
D000110:Acetylcholinesterase / Q000378:metabolism; D000287:Administration, Topical; D000818:Animals; D001285:Atropine / Q000008:administration & dosage / Q000494:pharmacology; D001794:Blood Pressure / Q000187:drug effects*; D002217:Carbachol / Q000037:antagonists & inhibitors / Q000494:pharmacology; D002415:Cats; D003864:Depression, Chemical; D004789:Enzyme Activation / Q000187:drug effects; D006339:Heart Rate / Q000187:drug effects; D007531:Isoflurophate / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D008526:Medulla Oblongata / Q000187:drug effects; D009768:Obidoxime Chloride / Q000494:pharmacology; D010091:Oximes / Q000494:pharmacology; D010862:Pilocarpine / Q000494:pharmacology; D011220:Pralidoxime Compounds / Q000494:pharmacology; D011437:Propylamines / Q000494:pharmacology; D011726:Pyridinium Compounds / Q000494:pharmacology; D012119:Respiration / Q000187:drug effects; D013997:Time Factors
D010091:Oximes; D011220:Pralidoxime Compounds; D011437:Propylamines; D011726:Pyridinium Compounds; D010862:Pilocarpine; D007531:Isoflurophate; D009768:Obidoxime Chloride; D001285:Atropine; D002217:Carbachol; D000110:Acetylcholinesterase