Basic studies for continuous production of L-aspartic acid by immobilized Escherichia coli cells.
By using a column packed with immobilized Escherichia coli cells entrapped in a polyacrylamide gel lattice, conditions for continuous production of L-aspartic acid from ammonium fumarate were investigated. When a solution of 1 M ammonium fumarate (pH 8.5) containing 1 mM Mg(2+) was passed through the immobilized cell column at a flow rate of space velocity (SV) = 0.8 at 37 C, the highest rate of reaction was attained. From the column effluents, L-aspartic acid was obtained in good yield. The immobilized cell column was very stable.
D000178:Acrylamides; D001224:Aspartic Acid / Q000032:analysis / Q000096:biosynthesis*; D001431:Bacteriological Techniques; D001681:Biological Assay; D002118:Calcium / Q000494:pharmacology; D004926:Escherichia coli / Q000302:isolation & purification / Q000378:metabolism*; D005650:Fumarates / Q000378:metabolism; D005782:Gels; D006863:Hydrogen-Ion Concentration; D007934:Leuconostoc; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D013237:Stereoisomerism; D013696:Temperature
D000178:Acrylamides; D005650:Fumarates; D005782:Gels; D000644:Quaternary Ammonium Compounds; D001224:Aspartic Acid; D008345:Manganese; D008274:Magnesium; D002118:Calcium