title: Interaction of Mycoplasma arthritidis and other mycoplasmas with murine peritoneal macrophages.
abstract: Neither mouse nor rat peritoneal macrophages were able to kill Mycoplasma arthritidis to any observable degree in the absence of specific hyperimmune rabbit antiserum. Although convalescent mouse and rat serum were somewhat inhibitory to M. arthritidis in the absence of macrophages, these sera did not promote active phagocytosis by peritoneal macrophages. In fact, the macrophages appeared to protect the mycoplasmas against the inhibitory effects of the antisera by stimulating their growth. Hyperimmune rabbit antiserum against M. arthritidis initiated phagocytic action and resulted in a 50-fold decrease in numbers of viable mycoplasmas by 6 h. In contrast with M. arthritidis, M. pulmonis rapidly adsorbed to the surface of peritoneal macrophages. Upon addition of specific rabbit antiserum, a rapid decrease in viable organisms occurred, and a more complete destruction of organisms ensued in comparison with M. arthritidis. M. gallinarum, as with M. arthritidis, did not adsorb to the macrophages to any great extent. Phagocytic action was observed only in the presence of homologous rabbit antiserum and was not marked until after 6 h of incubation.
mesh_terms: D000327:Adsorption; D000818:Animals; D001431:Bacteriological Techniques; D003168:Complement Fixation Tests; D003470:Culture Media; D007106:Immune Sera / Q000494:pharmacology; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D009174:Mycoplasma / Q000276:immunology*; D010529:Peritoneal Cavity / Q000166:cytology; D010587:Phagocytosis; D051381:Rats; D014357:Trypsin
chemical_list: D003470:Culture Media; D007106:Immune Sera; D014357:Trypsin