Class of phi chi 174 mutants relatively deficient in synthesis of viral RNA.
Nonpermissive cells infected with phiX174 gene D amber mutants synthesized some sixfold less viral RNA than permissive cells. The decrease was unaffected by increasing the multiplicity of infection and was a consequence of an overall decrease in all viral RNA species. It is suggested that the gene D product may function in replicative form DNA unwinding to expose the template for transcription.
D003090:Coliphages / Q000254:growth & development; D004267:DNA Viruses / Q000254:growth & development; D003673:Defective Viruses; D004926:Escherichia coli; D009154:Mutation; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis*; D014779:Virus Replication
D012333:RNA, Messenger; D012367:RNA, Viral