A new relaxed mutant of Escherichia coli with an altered 50S ribosomal subunit.
A new relaxed mutant called rel C has been isolated from a rel A(+)/rel A(+) partial diploid strain. The rel C mutant is unable to synthesize ppGpp or pppGpp in vivo in response to amino acid starvation or in vitro, but can synthesize these nucleotides in a shift-down. Rel C maps near rif. Studies in vitro demonstrate the lesion to be probably in one of the 50S ribosomal proteins that can be removed by 1.0 M LiCl.
D000596:Amino Acids / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D002874:Chromosome Mapping; D004926:Escherichia coli / Q000378:metabolism*; D005796:Genes; D006150:Guanine Nucleotides / Q000096:biosynthesis*; D008094:Lithium; D009154:Mutation; D010641:Phenotype; D012270:Ribosomes / Q000378:metabolism*; D013997:Time Factors
D000596:Amino Acids; D001426:Bacterial Proteins; D006150:Guanine Nucleotides; D008094:Lithium