Reuse of Salmonella and Shigella absorbing cells for preparing monospecific Salmonella O and Shigella antisera.
Salmonella and Shigella organisms used as absorbing cells for preparing group-or type-specific Salmonella O or Shigella antisera may be reused four or more times without qualitatively reducing their capacity to remove undesired antibodies. The cells may be reclaimed by heating in flowing steam or by treatment with N/5 HCl. The former method is preferred.
D000042:Absorption; D000372:Agglutination Tests; D000942:Antigens, Bacterial; D003429:Cross Reactions; D006358:Hot Temperature; D006851:Hydrochloric Acid; D007106:Immune Sera; D008722:Methods; D012475:Salmonella / Q000276:immunology*; D012703:Serotyping; D012763:Shigella flexneri / Q000276:immunology*
D000942:Antigens, Bacterial; D007106:Immune Sera; D006851:Hydrochloric Acid