Selective broth medium for isolation of group B streptococci.
A selective medium containing Todd-Hewitt broth, sheep blood, nalidixic acid, and gentamicin sulfate was found to enhance significantly the isolation of group B streptococci from vaginal cultures. Preparation of the medium, which is stable for up to 4 weeks at 4 C, is simple and inexpensive. Use of such a medium should facilitate identification of vaginal colonization with group B streptococci.
D000818:Animals; D001431:Bacteriological Techniques; D001769:Blood; D003470:Culture Media; D004926:Escherichia coli / Q000254:growth & development; D005069:Evaluation Studies as Topic; D005260:Female; D005839:Gentamicins; D006801:Humans; D007711:Klebsiella pneumoniae / Q000254:growth & development; D009268:Nalidixic Acid; D011247:Pregnancy; D012756:Sheep; D013210:Staphylococcus / Q000254:growth & development; D013291:Streptococcus / Q000254:growth & development / Q000302:isolation & purification*; D013292:Streptococcus agalactiae / Q000254:growth & development; D014621:Vagina / Q000382:microbiology*
D003470:Culture Media; D005839:Gentamicins; D009268:Nalidixic Acid