Axenic culture of myxamoebae of the myxomycete Physarum polycephalum.
Myxamoebae of the acellular slime mold Physarum polycephalum have been cultured axenically in a soluble medium. The growth medium contains bovine serum albumin, embryo extracts, liver infusion broth, peptone, and glucose. Cell densities ranging from 3 x 10(6) to 5 x 10(6) cells/ml have been obtained with this medium. To date, myxamoebae have been serially transferred more than 100 times without deleterious effect.
D000362:Agar; D000818:Animals; D002250:Carbon Radioisotopes; D002452:Cell Count; D002642:Chick Embryo; D003470:Culture Media; D004926:Escherichia coli; D005947:Glucose; D008858:Microscopy, Phase-Contrast; D009235:Myxomycetes / Q000254:growth & development* / Q000378:metabolism; D010461:Peptones; D011492:Protein Hydrolysates / Q000378:metabolism; D012710:Serum Albumin, Bovine; D014020:Tissue Extracts
D002250:Carbon Radioisotopes; D003470:Culture Media; D010461:Peptones; D011492:Protein Hydrolysates; D014020:Tissue Extracts; D012710:Serum Albumin, Bovine; D000362:Agar; D005947:Glucose