title: Roles of T and B lymphocytes in the termination of unresponsiveness to autologous thyroglobulin in mice.
abstract: The data presented in this paper support the hypothesis that unresponsiveness to autologous thyroglobulin (Tg) exists in the T cells and responsiveness exists in the B cells. Such a conclusion is based on the results of antigen-binding studies where few, if any, thymocytes recognized syngeneic Tg. Comparable numbers of antigen-binding lymphocytes for syngeneic Tg were found in the spleens of normal intact mice and of nude mice. The latter fact suggested that B cells exist which recognize self-constituents. From antigen-suicide experiments, a clearer picture of the susceptibility of B cells to iodinated self-antigen and of the obligatory role of antibody in the induction of lesions was developed. Only bone marrow cells (B cells) were affected by [(125)I]syngeneic Tg, in which case the incidence of lesions was diminished. From adoptive transfer experiments, the results demonstrate that unresponsiveness may be terminated by immunization with a mixture of heterologous (cross-reacting) Tg's. In this situation T cells are required since a B-cell reconstituted host failed to make antibody (plaque-forming cells) and to develop lesions. T cells in this form of an unresponsive state may recognize determinants on the heterologous Tg unrelated to autologous Tg and as such stimulate the normal complement of B cells to produce antibody that both reacts with autologous and heterologous Tg.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D001323:Autoantibodies / Q000096:biosynthesis*; D001345:Autoradiography; D001402:B-Lymphocytes / Q000276:immunology*; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D002417:Cattle; D005455:Fluorescent Antibody Technique; D006041:Goats / Q000276:immunology; D006462:Hemolytic Plaque Technique; D006736:Horses; D007106:Immune Sera / Q000302:isolation & purification; D007114:Immunization; D007140:Immunoglobulin Fab Fragments; D007274:Injections, Intraperitoneal; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D051379:Mice; D008815:Mice, Inbred Strains; D009584:Nitrogen / Q000032:analysis; D011817:Rabbits / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology; D013954:Thyroglobulin; D013961:Thyroid Gland / Q000473:pathology; D013966:Thyroiditis / Q000276:immunology* / Q000473:pathology
chemical_list: D001323:Autoantibodies; D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments; D007457:Iodine Radioisotopes; D013954:Thyroglobulin; D009584:Nitrogen