A mutant of Escherichia coli with a new, highly efficient promoter for the lactose operon.
We have isolated a mutant of E. coli whose maximal rate of synthesis of lac mRNA is 25-fold greater than that of its parental wild-type strain. The mutant also shows alterations in the kinetics of beta-galactosidase synthesis, the functional half-life of beta-galactosidase mRNA, and the properties of lac repressor.
D000123:Acetyltransferases / Q000096:biosynthesis; D004269:DNA, Bacterial; D004790:Enzyme Induction; D004926:Escherichia coli / Q000378:metabolism*; D005690:Galactose; D005696:Galactosidases / Q000096:biosynthesis; D005796:Genes; D007700:Kinetics; D007785:Lactose / Q000378:metabolism*; D009154:Mutation; D009693:Nucleic Acid Hybridization; D009876:Operon; D010761:Phosphorus Radioisotopes; D012329:RNA, Bacterial / Q000032:analysis / Q000096:biosynthesis; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D012293:Rifampin / Q000494:pharmacology; D013865:Thioglycosides; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D010761:Phosphorus Radioisotopes; D012329:RNA, Bacterial; D012333:RNA, Messenger; D013865:Thioglycosides; D000123:Acetyltransferases; D005696:Galactosidases; D007785:Lactose; D012293:Rifampin; D005690:Galactose