A hyperglycemic peptide from pituitary growth hormone: preparation with pepsin and assay in ob-ob mice.
A fragment that induces glucose intolerance in hereditarily obese mice has been prepared from pituitary growth hormone(sheep and human) by controlled digestion with pepsin in 0.05 M sodium acetate buffer, pH 3.7. The digest was purified by column chromatography on Bio-Gel P-6, followed by chromatography on Bio-Rex 70 and Bio-Gel P-2; in each successive step peptide was quantitated with the fluorescamine procedure. The fragment from sheep growth hormone has these characteristics: (1) It induces glucose intolerance in fasted ob/ob female mice when injected subcutaneously in a divided dose 15 min before, and concurrently with, glucose. (2) It is a basie peptide, as judged from its elution behavior on Bio-Rex 70, H(+) form. (3) It does not crossreact with antiserum to human growth hormone in radioimmunoassay. Further purification and initial sequence determinations have been undertaken.
D000818:Animals; D001681:Biological Assay; D001786:Blood Glucose; D002845:Chromatography; D005260:Female; D005951:Glucose Tolerance Test; D013006:Growth Hormone / Q000032:analysis* / Q000494:pharmacology; D006801:Humans; D006868:Hydrolysis; D006943:Hyperglycemia / Q000139:chemically induced; D051379:Mice; D008815:Mice, Inbred Strains; D009765:Obesity / Q000097:blood; D010434:Pepsin A; D010446:Peptide Fragments / Q000032:analysis* / Q000494:pharmacology; D012756:Sheep
D001786:Blood Glucose; D010446:Peptide Fragments; D013006:Growth Hormone; D010434:Pepsin A