Hybrid antibody-induced topographical redistribution of surface immunoglobulins, alloantigens, and concanavalin A receptors on mouse lymphoid cells.
Redistribution of surface immunoglobulins, H-2(b), Thy-1.2, and TL.1,2,3 alloantigens, and concanavalin A receptors on mouse lymphoid cells induced by hybrid rabbit F(ab')(2) antibody (anti-mouse immunoglobulin/anti-visual marker or anti-concanavalin A/anti-visual marker) was studied by immunofluorescence. When used directly to label surface immunoglobulin, and indirectly to label alloantigens and concanavalin A receptors, hybrid antibodies induced similar displacement of all surface components from a uniform distribution into "patches" and "caps" at 37 degrees . One hybrid antibody preparation, antimouse immunoglobulin/anti-ferritin, contained negligible amounts of bivalent anti-mouse immunoglobulin antibody, and was therefore "monovalent" for the antimouse immunoglobulin specificity. This observation suggests that factors other than multivalent crosslinking are responsible for hybrid antibody-induced redistribution of cell-surface components. Cap formation induced by hybrid antibody was enhanced markedly by attachment of the visual marker, either ferritin or southern bean mosaic virus, at 37 degrees . At -5 degrees , hybrid antibody does not displace uniformly distributed H-2(b) alloantigen-alloantibody complexes, but patches of label develop when ferritin attaches to the hybrid antibody. These results explain the patchy distribution of cell-surface components, which is a temperature-independent characteristic of labeling with hybrid antibodies and visual markers for electron microscopy.
D000818:Animals; D000906:Antibodies; D000914:Antibodies, Viral; D000937:Antigen-Antibody Reactions; D001666:Binding Sites, Antibody; D002462:Cell Membrane / Q000276:immunology*; D003208:Concanavalin A; D005293:Ferritins; D005455:Fluorescent Antibody Technique; D006041:Goats / Q000276:immunology; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007136:Immunoglobulins; D007519:Isoantigens; D008198:Lymph Nodes / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009029:Mosaic Viruses / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology
D000906:Antibodies; D000914:Antibodies, Viral; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007136:Immunoglobulins; D007519:Isoantigens; D003208:Concanavalin A; D005293:Ferritins