Serum gentamicin assay: a comparison and assessment of different methods.
We have compared, in a clinical laboratory, three methods for estimating the concentration of gentamicin in serum. The adenylase method is most accurate, but requires considerable skilled technical time and expensive apparatus. The urease method requires an accurate pH meter but is otherwise inexpensive, but in our hands, although it produces results most rapidly, it also requires considerable technician time and is least accurate. The agar diffusion method requires no expensive apparatus, least technician time, and produces results of acceptable accuracy. It does, however, take longer than the other two methods to produce results.
D000704:Analysis of Variance; D001681:Biological Assay / Q000379:methods; D002250:Carbon Radioisotopes; D005839:Gentamicins / Q000097:blood*; D006801:Humans; D009713:Nucleotidyltransferases; D011513:Proteus mirabilis / Q000187:drug effects; D012706:Serratia marcescens / Q000187:drug effects; D013997:Time Factors; D014510:Urease
D002250:Carbon Radioisotopes; D005839:Gentamicins; D009713:Nucleotidyltransferases; D014510:Urease