title: Purification and physical characterization of T7 RNA polymerase from T7-infected Escherichia coli B.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000645:Ammonium Sulfate; D001389:Azirines; D002499:Centrifugation, Density Gradient; D002845:Chromatography; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003090:Coliphages / Q000201:enzymology*; D004267:DNA Viruses / Q000201:enzymology; D004279:DNA, Viral / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000032:analysis / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000096:biosynthesis; D013698:Templates, Genetic; D014158:Transcription, Genetic; D014461:Ultracentrifugation; D014776:Virus Cultivation
chemical_list: D000596:Amino Acids; D001389:Azirines; D004279:DNA, Viral; D012367:RNA, Viral; D012321:DNA-Directed RNA Polymerases; D000645:Ammonium Sulfate