Isolation and characterization of an Escherichia coli K-12 mutant with a temperature-sensitive recA- phenotype.
A mutation that causes a temperature-sensitive RecA(-) phenotype was identified in a derivative of a PolA(-) strain that failed to grow at high temperature. The mutant allele (recA200) was shown to be linked to cysC, conferred a sharply temperature-sensitive, ultraviolet-sensitive Rec(-) phenotype in the range 35 to 42 C, and in crosses failed to show complementation at 42 C with Hfr's that transferred recA(-). Double mutants that carried both recA200 and polA were examined for ability to grow and synthesize DNA at restrictive temperatures.
D000483:Alleles; D002470:Cell Survival; D002874:Chromosome Mapping; D003227:Conjugation, Genetic; D004254:DNA Nucleotidyltransferases / Q000096:biosynthesis; D004269:DNA, Bacterial / Q000096:biosynthesis; D004926:Escherichia coli / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism / Q000528:radiation effects; D005796:Genes; D005816:Genetic Complementation Test; D008040:Genetic Linkage; D009153:Mutagens; D009154:Mutation; D009604:Nitrosoguanidines; D010641:Phenotype; D011830:Radiation Effects; D011995:Recombination, Genetic; D013696:Temperature; D013936:Thymidine / Q000378:metabolism; D014161:Transduction, Genetic; D014316:Tritium; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D009153:Mutagens; D009604:Nitrosoguanidines; D014316:Tritium; D004254:DNA Nucleotidyltransferases; D013936:Thymidine