Isolation of specialized transducing bacteriophages for gluconate 6-phosphate dehydrogenase (gnd) of Escherichia coli.
Specialized transducing phages for gluconate 6-phosphate dehydrogenase (gnd), a constitutive enzyme in Escherichia coli, have been isolated using a method previously described for other genes. The gnd-his region, carried on an F' episome, was first transposed to tonB. Rare phages carrying gnd were selected, by transduction, from phi80 lysogens of these strains; one phage also carried his (phi80gndhis). From the transductants, high-frequency transducing lysates were obtained; low multiplicity of infection then yielded defective lysogens. tonB deletion analysis of the phi80dgndhis lysogen shows the order of genes in the prophage to be imm80...hisOGD...gnd; according to a marker rescue experiment most phage late genes have been replaced by bacterial deoxyribonucleic acid. A heat-inducible, lysis-defective lambda-phi80 hybrid derivative of phi80dgndhis has been prepared.
D002874:Chromosome Mapping; D003090:Coliphages / Q000302:isolation & purification* / Q000528:radiation effects; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism; D005796:Genes; D005942:Gluconates / Q000378:metabolism; D008242:Lysogeny; D009154:Mutation; D010734:Phosphogluconate Dehydrogenase / Q000096:biosynthesis*; D011830:Radiation Effects; D011995:Recombination, Genetic; D014161:Transduction, Genetic; D014466:Ultraviolet Rays
D005942:Gluconates; D010734:Phosphogluconate Dehydrogenase