Rapid methods for biochemical testing of anaerobic bacteria.
Rapid biochemical tests for nitrate, indole, gelatin, starch, esculin, and o-nitrophenyl-beta-D-galactopyranoside were performed on 112 strains of anaerobic bacteria. All tests were incubated under aerobic conditions, and results were recorded within 4 h. The tests for nitrate, indole, and starch showed a 95% or greater correlation when compared to the standard biochemical tests. Tests for esculin and gelatin showed an agreement of 86 and 77%, respectively. PathoTec test strips for nitrate, indole, esculin, o-nitrophenyl-beta-D-galactopyranoside, Voges-Proskauer, and urease were also tested and showed encouraging results.
D000693:Anaerobiosis; D001419:Bacteria / Q000145:classification* / Q000378:metabolism; D001431:Bacteriological Techniques; D004929:Esculin / Q000378:metabolism; D005069:Evaluation Studies as Topic; D005690:Galactose; D005696:Galactosidases / Q000378:metabolism; D005780:Gelatin / Q000378:metabolism; D006027:Glycosides / Q000378:metabolism; D006868:Hydrolysis; D007211:Indoles / Q000096:biosynthesis; D009566:Nitrates / Q000378:metabolism; D009578:Nitrobenzenes; D010084:Oxidation-Reduction; D011934:Reagent Strips; D013213:Starch / Q000378:metabolism
D006027:Glycosides; D007211:Indoles; D009566:Nitrates; D009578:Nitrobenzenes; D011934:Reagent Strips; D004929:Esculin; D005780:Gelatin; D013213:Starch; D005696:Galactosidases; D005690:Galactose