Isolation and characterization of specialized phi80 transducing phages carrying regions of the Salmonella typhimurium trp operon.
We have isolated a series of nondefective phi80 specialized transducing phage which carry segments of the Salmonella typhimurium trp operon. These phage were obtained from a lysogenic derivative of a merozygote constructed by transferring an S. typhimurium trp episome into an Escherichia coli strain which lacks the normal phi80 attachment site. The deoxyribonucleic acid (DNA) from one such phage was purified and employed in DNA-ribonucleic acid (RNA) hybridization studies. The results obtained show that, under our hybridization conditions, heterologous hybridization is less efficient than homologous hybridization. It was also observed that not all S. typhimurium trp messenger RNA can readily anneal to E. coli trp operon DNA. Heterologous hybrids consisting of S. typhimurium trp messenger RNA and E. coli trp operon DNA were estimated to have a dissociation constant 10-fold larger than that of homologous hybrids.
D002874:Chromosome Mapping; D003090:Coliphages / Q000254:growth & development / Q000302:isolation & purification*; D004267:DNA Viruses; D004279:DNA, Viral; D004926:Escherichia coli / Q000378:metabolism; D008242:Lysogeny; D008937:Mitomycins; D009154:Mutation; D009693:Nucleic Acid Hybridization; D009876:Operon; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012486:Salmonella typhimurium / Q000378:metabolism*; D014161:Transduction, Genetic; D014316:Tritium; D014364:Tryptophan / Q000096:biosynthesis*; D014466:Ultraviolet Rays
D004279:DNA, Viral; D008937:Mitomycins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D014316:Tritium; D014364:Tryptophan