Microadaption of hemadsorption inhibition for neutralization tests with pig hemagglutinating encephalomyelitis virus.
A microneutralization test is described, in which secondary pig thyroid cells are used and end points are determined by hemadsorption with hamster erythrocytes. The method is evaluated and compared with a macromethod, in which the fluorescent-antibody staining technique is applied for reading titers. The micromethod was found to be useful because of its sensitivity and rapidity.
D000704:Analysis of Variance; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002460:Cell Line; D003332:Coronaviridae / Q000276:immunology*; D006224:Cricetinae / Q000276:immunology; D004679:Encephalomyelitis / Q000382:microbiology*; D004912:Erythrocytes / Q000276:immunology; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D006382:Hemadsorption; D006389:Hemagglutinins, Viral / Q000032:analysis*; D009500:Neutralization Tests / Q000379:methods*; D013552:Swine; D013961:Thyroid Gland; D014776:Virus Cultivation
D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral