Evidence for heterogeneity in populations of T5 bacteriophage.
Each T5 stock contains a population of particular phages that, just after adsorption onto the host bacteria. release their entire chromosome outside the bacterial membrane in a place where it is sensitive to bacterial enzymes. This release takes place before the sensitization step to deprivation of calcium and before the transfer of the first-step DNA fragment. Secondarily, this released DNA is degraded by bacterial enzymes, mainly by the endonuclease I; the products of degradation are spontaneously released in the surrounding medium. Thus, in each T5 phage stock it seems that there is a minor population that is deficient for the mechanism of controlled DNA injection into the bacteria.
D000327:Adsorption; D001665:Binding Sites; D002250:Carbon Radioisotopes; D002499:Centrifugation, Density Gradient; D003090:Coliphages; D004267:DNA Viruses; D004279:DNA, Viral; D004926:Escherichia coli; D014316:Tritium
D002250:Carbon Radioisotopes; D004279:DNA, Viral; D014316:Tritium