Acceptor activity of hypermethylated E. coli tRNAf-Met.
The acceptor activity of normal E. coli tRNA(Met) (f) was compared with that of a preparation with surplus methyl groups introduced by a crude methylase preparation from rat hepatoma. No changes in charging were detected when the aminoacylation was carried out in a homologous system. The data indicate that neither the surplus methyl groups by themselves, nor the eventual changes in spatial arrangement are essential for charging of E. coli tRNA(Met) (f).
D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D001483:Base Sequence; D002250:Carbon Radioisotopes; D002855:Chromatography, Thin Layer; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D005561:Formates; D007553:Isotope Labeling; D008715:Methionine / Q000378:metabolism; D008745:Methylation; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000032:analysis; D012329:RNA, Bacterial / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D012265:Ribonucleotides / Q000032:analysis; D012436:S-Adenosylmethionine / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors; D014316:Tritium
D002250:Carbon Radioisotopes; D005561:Formates; D009841:Oligonucleotides; D012329:RNA, Bacterial; D012265:Ribonucleotides; D014316:Tritium; D012436:S-Adenosylmethionine; D012343:RNA, Transfer; D008715:Methionine; D000604:Amino Acyl-tRNA Synthetases