Release of DNA in circulating blood and induction of anti-DNA antibodies after injection of bacterial lipopolysaccharides.
The present data demonstrate the induction of antisingle-stranded (SS) DNA and antidouble-stranded DNA antibodies in various strains of mice, including athymic C57BL/6 nude mice, after the injection of bacterial lipopolysaccharide (LPS). This anti-DNA response is dose dependent and varies quantitatively according to the strain of the injected mice. It is not correlated to the H-2 histocompatibility locus nor to the immune response to LPS. The lipid A fraction appears to be the active part of the LPS molecule for this particular effect. In addition, it was found that DNA is released in circulating blood a few hours after the injection of LPS. Most of the DNA released has physicochemical and immunochemical characteristics of SS DNA. Therefore, the anti-DNA response induced by injections of LPS may be the result of a release of DNA in a particularly immunogenic form at a time when the immune system, in particular the B lymphocytes, is rendered capable by LPS of developing an immune response to such a soluble antigen. These effects of LPS may account for the triggering or the exacerbation of ante-DNA antibodies during infections with gram-negative bacteria, and a similar mechanism may be involved in the pathogenesis of systemic lupus erythematosus.
D000818:Animals; D000974:Antibodies, Antinuclear; D000917:Antibody Formation; D000918:Antibody Specificity; D000942:Antigens, Bacterial; D002850:Chromatography, Gel; D004247:DNA / Q000097:blood / Q000276:immunology*; D004277:DNA, Single-Stranded / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology; D006824:Hybridization, Genetic; D007114:Immunization; D008070:Lipopolysaccharides / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D011863:Radioimmunoassay; D012477:Salmonella enteritidis / Q000276:immunology; D012486:Salmonella typhimurium / Q000276:immunology
D000974:Antibodies, Antinuclear; D000942:Antigens, Bacterial; D004277:DNA, Single-Stranded; D008070:Lipopolysaccharides; D004247:DNA