Applicability of the reverse-flow filter technique to marine microbial studies.
The use of the reverse-flow filtration technique to quantitatively concentrate marine bacteria was evaluated using both a pure culture and seawater samples. Our data indicate that the cells are altered during the filtration procedure and that a significant and inconsistent number of cells are lost on the membrane filter. The results obtained indicate that data on marine bacteria concentrated in this manner should be interpreted with caution.
D001431:Bacteriological Techniques; D002250:Carbon Radioisotopes; D002452:Cell Count; D003470:Culture Media; D005374:Filtration; D005971:Glutamates / Q000378:metabolism; D007700:Kinetics; D008722:Methods; D008851:Micropore Filters; D011392:Proline / Q000378:metabolism; D012623:Seawater; D014733:Vibrio / Q000254:growth & development / Q000378:metabolism; D014871:Water Microbiology
D002250:Carbon Radioisotopes; D003470:Culture Media; D005971:Glutamates; D011392:Proline