title: A simplified method for preparing sucrose gradients.
abstract: Numerous sucrose gradients can be prepared simultaneously by diffusion in horizontal centrifuge tubes.
mesh_terms: D002499:Centrifugation, Density Gradient; D004269:DNA, Bacterial; D004270:DNA, Circular; D004058:Diffusion; D004926:Escherichia coli; D005069:Evaluation Studies as Topic; D008722:Methods; D013395:Sucrose; D013696:Temperature; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial; D004270:DNA, Circular; D013395:Sucrose