title: Replacement of ribosomal protein S1 by interference factor ialpha in ribosomal binding of phage Ms2 RNA.
abstract: The MS2 RNA binding capacity of 30S ribosomal subunits, which is lost when protein S1 is removed, can be restored following incubation with interference factor ialpha and repelleting. Polyacrylamide-agarose gel electrophoresis shows that, under these conditions, a faster moving, non-RNA binding 30S species, which contains no S1, is converted to a slower moving RNA-binding one, having the same mobility as the 30S species that contains protein S1. Factor ialpha binds to single-stranded RNAs in a pattern that closely resembles the RNA binding pattern of initiation factor IF-3.
mesh_terms: D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002250:Carbon Radioisotopes; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism* / Q000648:ultrastructure; D010442:Peptide Chain Initiation, Translational; D011072:Poly U / Q000378:metabolism; D011119:Polynucleotides / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D014316:Tritium; D014764:Viral Proteins / Q000096:biosynthesis
chemical_list: D001426:Bacterial Proteins; D002250:Carbon Radioisotopes; D011119:Polynucleotides; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium; D011072:Poly U