title: Negative cooperativity and half of the sites reactivity. Alkaline phosphatases of Escherichia coli with Zn2+, Co2+, Cd2+, Mn2+, and Cu2+ in the active sites.
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis / Q000378:metabolism*; D001059:Apoproteins; D001665:Binding Sites; D002104:Cadmium / Q000378:metabolism; D002384:Catalysis; D002413:Cations, Divalent; D003035:Cobalt / Q000378:metabolism; D003300:Copper / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology*; D005994:Glycerophosphates / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D046911:Macromolecular Substances; D008345:Manganese / Q000378:metabolism; D008667:Metalloproteins / Q000378:metabolism; D009578:Nitrobenzenes / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D009991:Oscillometry; D010710:Phosphates / Q000032:analysis / Q000378:metabolism; D013053:Spectrophotometry; D013329:Structure-Activity Relationship; D014131:Trace Elements / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism
chemical_list: D001059:Apoproteins; D002413:Cations, Divalent; D005994:Glycerophosphates; D046911:Macromolecular Substances; D008667:Metalloproteins; D009578:Nitrobenzenes; D009943:Organophosphorus Compounds; D010710:Phosphates; D014131:Trace Elements; D002104:Cadmium; D003035:Cobalt; D008345:Manganese; D003300:Copper; D000469:Alkaline Phosphatase; D015032:Zinc