Escherichia coli females defective in conjugation and in adsorption of a single-stranded deoxyribonucleic acid phage.
We predicted that, among mutants resistant to infection by single-stranded deoxyribonucleic acid viruses, there would be some also resistant to "infection" by single-stranded conjugal deoxyribonucleic acid. Approximately 5% of the Escherichia coli K-12 females selected for resistance to phage ST-1 were defective as recipients in conjugation. These spontaneous mutants fell into two classes. Type A accepted both plasmid and chromosomal markers at greatly reduced frequencies (<10(-6) of normal for at least one strain), formed "rough" colonies, and (unlike their parent) were nonflagellated. Type B strains accepted both chromosomal and plasmid markers at reduced frequencies (10(-2) to 10(-1) of normal), were temperature sensitive for growth, and showed increased susceptibility towards antibiotics and deoxycholate. Both classes of mutants also were resistant to certain female-specific viruses.
D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis; D002250:Carbon Radioisotopes; D003090:Coliphages / Q000254:growth & development*; D003227:Conjugation, Genetic; D004267:DNA Viruses; D004269:DNA, Bacterial / Q000096:biosynthesis; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D003840:Deoxycholic Acid / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D005111:Extrachromosomal Inheritance; D005407:Flagella; D007930:Leucine / Q000378:metabolism; D009154:Mutation; D013696:Temperature; D013936:Thymidine / Q000378:metabolism
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002250:Carbon Radioisotopes; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D003840:Deoxycholic Acid; D007930:Leucine; D013936:Thymidine