Template properties of glucose-deficient T-even bacteriophage DNA.
The vegetative DNA isolated from T4-infected Escherichia coli W4597 (UDPG PPase(-)) was about two to six times more active in stimulating protein synthesis in cell-free extracts than that isolated from T4-infected E. coli B06. This suggested that nonglucosylated vegetative DNA may be a better template than the glucosylated form. This view was supported by experiments measuring RNA synthesis on mature T-even DNAs with a range of glucose contents. The extent of (14)C-GTP polymerization was inversely proportional to the glucose content of the DNA. Differences were also observed in both the kind and quantity of polypeptides produced in response to these DNAs.
D000596:Amino Acids / Q000378:metabolism; D001345:Autoradiography; D002250:Carbon Radioisotopes; D002474:Cell-Free System; D003090:Coliphages / Q000032:analysis / Q000254:growth & development*; D004279:DNA, Viral / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D005947:Glucose / Q000032:analysis*; D006160:Guanosine Triphosphate / Q000378:metabolism; D009154:Mutation; D012367:RNA, Viral / Q000096:biosynthesis; D012762:Shigella dysenteriae; D013698:Templates, Genetic; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
D000596:Amino Acids; D002250:Carbon Radioisotopes; D004279:DNA, Viral; D012367:RNA, Viral; D014764:Viral Proteins; D006160:Guanosine Triphosphate; D005947:Glucose