Regulation of the pool size of valine in Escherichia coli K-12.
Three mutations (ilvH611, ilvH612, and ilvH613) are described which make Escherichia coli K-12 resistant to valine inhibition and are located near leu. The expression of the ilv genes appears to be normal in these mutants since the isoleucine-valine biosynthetic enzymes are not derepressed relative to the wild type. The intracellular concentration of valine is, however, higher in the mutants than in the isogenic ilvH(+) strain. These mutants also excrete valine, probably because of the high intracellular concentration of this amino acid. The pool size of valine is regulated independently from that of isoleucine and leucine. The increased intracellular concentration of valine is due to a decreased feedback inhibition that valine exerts on its own biosynthetic pathway. In fact, acetolactate synthase activity assayed in extracts of ilvH612 and ilvH613 mutants is more resistant to valine inhibition than the activity assayed in the ilvH(+) isogenic strain. Two forms of acetolactate synthase activity can be separated from these extracts by adsorption and elution on hydroxylapatite. One of them is as sensitive to valine inhibition as that of the wild type, the other is more resistant to valine inhibition.
D002474:Cell-Free System; D002874:Chromosome Mapping; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D005796:Genes; D006836:Hydro-Lyases / Q000378:metabolism; D007532:Isoleucine / Q000096:biosynthesis; D007535:Isomerases / Q000378:metabolism; D007930:Leucine / Q000096:biosynthesis; D009153:Mutagens; D009154:Mutation; D009604:Nitrosoguanidines; D007652:Oxo-Acid-Lyases / Q000378:metabolism; D010641:Phenotype; D013912:Threonine; D014161:Transduction, Genetic; D014466:Ultraviolet Rays; D014633:Valine / Q000096:biosynthesis / Q000378:metabolism* / Q000494:pharmacology
D009153:Mutagens; D009604:Nitrosoguanidines; D007532:Isoleucine; D013912:Threonine; D007652:Oxo-Acid-Lyases; D006836:Hydro-Lyases; D007535:Isomerases; D007930:Leucine; D014633:Valine