A new procedure to prepare highly purified and crystallized yeast pyruvate decarboxylase.
D001051:Apoenzymes / Q000302:isolation & purification; D002262:Carboxy-Lyases / Q000302:isolation & purification* / Q000378:metabolism; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003460:Crystallization; D003600:Cytosol / Q000201:enzymology; D004586:Electrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D008722:Methods; D008858:Microscopy, Phase-Contrast; D011134:Polysaccharides; D011773:Pyruvates; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology*; D013056:Spectrophotometry, Ultraviolet
D001051:Apoenzymes; D007527:Isoenzymes; D011134:Polysaccharides; D011773:Pyruvates; D002262:Carboxy-Lyases