Modification of essential histidyl residues of the beta 2 subunit of tryptophan synthetase by photo-oxidation in the presence of pyridoxal 5'-phosphate and L-serine and by diethylpyrocarbonate.
D000596:Amino Acids / Q000032:analysis; D004789:Enzyme Activation; D004926:Escherichia coli / Q000201:enzymology*; D004952:Esters; D005561:Formates / Q000494:pharmacology*; D006639:Histidine / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006898:Hydroxylamines / Q000494:pharmacology; D007700:Kinetics; D046911:Macromolecular Substances; D008956:Models, Chemical; D010084:Oxidation-Reduction; D010777:Photochemistry; D011485:Protein Binding; D011522:Protons; D011732:Pyridoxal Phosphate / Q000494:pharmacology*; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D012545:Schiff Bases; D012694:Serine / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors; D014367:Tryptophan Synthase / Q000032:analysis / Q000037:antagonists & inhibitors / Q000378:metabolism*
D000596:Amino Acids; D004952:Esters; D005561:Formates; D006898:Hydroxylamines; D046911:Macromolecular Substances; D011522:Protons; D000644:Quaternary Ammonium Compounds; D012545:Schiff Bases; D012694:Serine; D006639:Histidine; D011732:Pyridoxal Phosphate; D014367:Tryptophan Synthase