Cell-free synthesis of a specific lipoprotein of the Escherichia coli outer membrane directed by purified messenger RNA.
A specific lipoprotein of the E. coli outer membrane has been synthesized in a cell-free system directed by purified messenger RNA. The mRNA for the lipoprotein was purified as 7S RNA about 250-fold from exponentially growing cells. Protein synthesis of the cell-free system was totally dependent upon the addition of the purified mRNA. The product of the cell-free system was identified as the specific lipoprotein by immunoprecipitation and by peptide mapping.
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis*; D002462:Cell Membrane / Q000378:metabolism; D002474:Cell-Free System; D002850:Chromatography, Gel; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000648:ultrastructure*; D006868:Hydrolysis; D008074:Lipoproteins / Q000096:biosynthesis* / Q000276:immunology; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D011233:Precipitin Tests; D012329:RNA, Bacterial / Q000302:isolation & purification / Q000378:metabolism*; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*; D013462:Sulfur Radioisotopes
D001426:Bacterial Proteins; D008074:Lipoproteins; D010446:Peptide Fragments; D012329:RNA, Bacterial; D012333:RNA, Messenger; D013462:Sulfur Radioisotopes; D008715:Methionine; D003488:Cyanogen Bromide