title: Role for free isoleucine of glycyl-leucine in the repression of threonine deaminase in Escherichia coli.
abstract: In Escherichia coli, the three branched-chain amino acid activating enzymes appear to be essential for multivalent repression of the isoleucine- and valine-forming enzymes. The results of experiments with a mutant, strain CU18, having an altered threonine deaminase, indicate that free isoleucine and some form of threonine deaminase (the product of the ilvA gene) are also involved in multivalent repression. This strain exhibits abnormally high derepressibility but normal repressibility of its ilv gene products, and its threonine deaminase is inhibited only by high concentrations of isoleucine. In strain CU18, the isoleucine analogue, thiaisoleucine, is incapable of replacing isoleucine in the multivalent repression of the ilv genes, whereas the analogue can fully replace the natural amino acid in repression in other strains examined. The dipeptide, glycyl-leucine, which, like isoleucine, is a heterotropic negative effector of threonine deaminase but is not a substrate for isoleucyl-transfer ribonucleic acid synthetase, can completely prevent the accumulation of threonine deaminase-forming potential during isoleucine starvation in strains with normal threonine deaminases. It can not, however, prevent such accumulation in strain CU18 or in other strains in which threonine deaminase is insensitive to any concentration of isoleucine.
mesh_terms: D002474:Cell-Free System; D004151:Dipeptides / Q000494:pharmacology*; D004794:Enzyme Repression / Q000187:drug effects; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism; D005796:Genes; D005998:Glycine; D006836:Hydro-Lyases / Q000378:metabolism*; D007532:Isoleucine / Q000378:metabolism / Q000494:pharmacology*; D007930:Leucine; D009154:Mutation; D013912:Threonine; D014633:Valine / Q000378:metabolism
chemical_list: D004151:Dipeptides; D007532:Isoleucine; D013912:Threonine; D006836:Hydro-Lyases; D007930:Leucine; D014633:Valine; D005998:Glycine