Synthesis of a large precursor to ribosomal RNA in a mutant of Escherichia coli.
A mutant of E. coli, isolated by Kindler and Hofschneider as a strain defective in RNase III activity, forms a 30S precursor of ribosomal RNA ("30S pre-rRNA"). The half-life of the 30S pre-rRNA in growing cells at 30 degrees , estimated by the rate of specific (3)[H]uridine incorporation, is about 1 min. In rifampicin-treated cells, the RNA is metabolized to mature rRNA with a half-life of about 2 min. The 30S pre-rRNA has been highly purified. DNA-RNA hybridization tests demonstrate that it contains both 16S and 23S rRNA sequences. Also, in cultures treated with rifampicin, the cleavage products of radioactive 30S pre-rRNA include 25S and 17.5S RNA species, destined to becomes 23S and 16S rRNA. Thus, each 30S chain probably contains one 16S and one 23S RNA sequence, as well as additional sequences. Two independent techniques indicate that the additional portions account for about 27% of the total lenght: (1) By comparison to the sedimentation rate and electrophoretic mobility of marker RNAs, the 30S pre-RNA has an apparent molecular weight of 2.3 x 10(6) +/- 5%, or 28% more than the sum of 16S and 23S rRNA; (2) 27% of the 30S pre-rRNA is not competed away from hybridization by mature 16S and 23S rRNA.Thus, bacteria appear to make a pre-rRNA similar in some respects to that observed in eukaryotes; though in normal E. coli cells, the pre-rRNA is ordinarily cleaved endonucleolytically during its formation.
D002247:Carbon Isotopes; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D012329:RNA, Bacterial / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D012335:RNA, Ribosomal / Q000096:biosynthesis*; D012265:Ribonucleotides / Q000096:biosynthesis*; D012293:Rifampin / Q000494:pharmacology; D014316:Tritium; D014498:Uracil / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D002247:Carbon Isotopes; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012265:Ribonucleotides; D014316:Tritium; D014498:Uracil; D012293:Rifampin; D014529:Uridine