title: Gradient technique to test the effects of substances on fluorescent antibody reactions.
abstract: A simple technique is described for making gradients of substances, applying them to bacterial cells during fluorescent antibody reactions, and observing their effects.
mesh_terms: D000937:Antigen-Antibody Reactions / Q000187:drug effects*; D000942:Antigens, Bacterial; D001419:Bacteria / Q000276:immunology*; D002118:Calcium / Q000494:pharmacology; D002499:Centrifugation, Density Gradient / Q000295:instrumentation; D005455:Fluorescent Antibody Technique / Q000295:instrumentation*; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D008722:Methods
chemical_list: D000942:Antigens, Bacterial; D008274:Magnesium; D002118:Calcium