Deoxysubstitution in RNA by RNA polymerase in vitro: a new approach to nucleotide sequence determinations.
Deoxynucleotides have been incorporated into RNA synthesized in vitro by RNA polymerase with either double-stranded or single-stranded DNA as a template. By use of this technique to block or promote cleavage at a particular phosphodiester bond, a variety of specific cleavages may be obtained with the available ribonucleases and deoxyribonuclease I. These methods should greatly increase the ease and rapidity of nucleotide sequence determinations.
D001345:Autoradiography; D001483:Base Sequence; D004247:DNA; D004277:DNA, Single-Stranded; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism; D003854:Deoxyribonucleotides / Q000378:metabolism*; D004586:Electrophoresis; D004926:Escherichia coli / Q000201:enzymology; D009841:Oligonucleotides / Q000032:analysis; D010179:Pancreas / Q000201:enzymology; D010761:Phosphorus Radioisotopes; D012313:RNA / Q000378:metabolism*; D013698:Templates, Genetic
D004277:DNA, Single-Stranded; D003854:Deoxyribonucleotides; D009841:Oligonucleotides; D010761:Phosphorus Radioisotopes; D012313:RNA; D004247:DNA; D012321:DNA-Directed RNA Polymerases; D003851:Deoxyribonucleases