title: A new transfer RNA fragment reaction: Tp psi pCpGp bound to a ribosome-messenger RNA complex induces the synthesis of guanosine tetra- and pentaphosphates.
abstract: The tetranucleotide TpPsipCpGp, when bound to Escherichia coli 50S ribosomal subunits, can replace intact tRNA in the stringent factor-directed synthesis of guanosine tetra- and pentaphosphates. The TpPsipCpGp-dependent fragment reaction has a strict requirement for the 50S and 30S ribosomal subunits and synthetic or natural messenger RNA.
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis; D001665:Binding Sites; D002850:Chromatography, Gel; D003864:Depression, Chemical; D004926:Escherichia coli / Q000378:metabolism; D006150:Guanine Nucleotides / Q000096:biosynthesis*; D009841:Oligonucleotides / Q000378:metabolism*; D011485:Protein Binding; D011691:Puromycin / Q000494:pharmacology; D012329:RNA, Bacterial / Q000096:biosynthesis / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D006150:Guanine Nucleotides; D009841:Oligonucleotides; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D011691:Puromycin; D012343:RNA, Transfer