An improved method for the isolation of yeast nuclei active in RNA synthesis in vitro.
D002458:Cell Fractionation; D002467:Cell Nucleus / Q000032:analysis / Q000201:enzymology*; D002499:Centrifugation, Density Gradient; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D005069:Evaluation Studies as Topic; D008722:Methods; D008858:Microscopy, Phase-Contrast; D011205:Povidone; D012313:RNA / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology*; D013012:Sorbitol; D013056:Spectrophotometry, Ultraviolet; D014316:Tritium; D014500:Uracil Nucleotides
D014500:Uracil Nucleotides; D014316:Tritium; D013012:Sorbitol; D012313:RNA; D012321:DNA-Directed RNA Polymerases; D011205:Povidone