Anaerobic transport in Escherichia coli membrane vesicles.
Anaerobic beta-galactoside transport in whole cells and membrane vesicles from E. coli ML 308-225 is coupled to the oxidation of alpha-glycerol-P or D-lactate with fumarate as an electron acceptor. Alternatively, anaerobic beta-galactoside transport may be coupled to the oxidation of formate utilizing nitrate as electron acceptor. Both anaerobic electron-transfer systems are induced by growth of the organisms under appropriate conditions. Components of both systems are loosely bound to the membrane, necessitating the use of a modified procedure for vesicle preparation in order to demonstrate anaerobic transport in vitro. Addition of ATP or an ATP-generating system to vesicles prepared from anaerobically-grown cells or inclusion of ATP or the ATP-generating system during preparation of vesicles does not stimulate transport. The results support the conclusion that active transport under anaerobic conditions is coupled primarily to electron flow.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000693:Anaerobiosis; D001692:Biological Transport / Q000187:drug effects; D002250:Carbon Radioisotopes; D002462:Cell Membrane / Q000378:metabolism; D003486:Cyanides / Q000494:pharmacology; D004579:Electron Transport; D004926:Escherichia coli / Q000166:cytology / Q000378:metabolism*; D005561:Formates / Q000494:pharmacology; D005650:Fumarates / Q000494:pharmacology; D005690:Galactose / Q000378:metabolism; D005697:Galactosides; D005990:Glycerol / Q000494:pharmacology; D006027:Glycosides / Q000378:metabolism; D007785:Lactose / Q000378:metabolism*; D008854:Microscopy, Electron; D009566:Nitrates / Q000494:pharmacology
D002250:Carbon Radioisotopes; D003486:Cyanides; D005561:Formates; D005650:Fumarates; D005697:Galactosides; D006027:Glycosides; D009566:Nitrates; C007816:beta-galactoside; D000255:Adenosine Triphosphate; D007785:Lactose; D005990:Glycerol; D005690:Galactose