The application of the indirect fluorescent antibody test to samples of sera and dried blood from cattle in the Lambwe Valley, South Nyanza, Kenya.
The indirect fluorescent antibody test (IFT) has been used on a number of occasions to determine the level of trypanosomal antibodies in herds of cattle. In the study reported here, comparisons were made between IFTs applied to diluted cattle sera and to dried whole blood on filter paper, and between these studies and the results of a parasitological survey. The results showed that sera diluted 1: 160 gave IFT responses similar to those obtained from dried blood spots. However, neither test could be used for the diagnosis of individual trypanosome infections, although the IFT was clearly of value in indicating whether or not a herd of cattle had a history of exposure to infection.
D000367:Age Factors; D000818:Animals; D000829:Animals, Domestic; D002417:Cattle; D005260:Female; D005455:Fluorescent Antibody Technique; D007630:Kenya; D008297:Male; D012737:Sex Factors; D014346:Trypanosoma brucei brucei / Q000302:isolation & purification; D014354:Trypanosomiasis, Bovine / Q000097:blood* / Q000175:diagnosis / Q000276:immunology