Mutants of Escherichia coli defective in membrane phospholipid synthesis: mapping of the structural gene for L-glycerol 3-phosphate dehydrogenase.
The structural gene for the biosynthetic l-glycerol 3-phosphate dehydrogenase has been mapped at min 71.5 on the Escherichia coli chromosome. This gene (gpsA) is co-transduced with the xyl, mtl, and pyrE loci. Three-factor conjugational crosses and the transduction data indicate that the order of loci in this region of the chromosone is mtl, gltE, gpsA, gadR, gadS, pyrE. Study of a temperature-sensitive gpsA mutant possessing a dehydrogenase of increased thermolability indicated that gpsA is the structural gene for the dehydrogenase. All dehydrogenase-deficient strains tested were mapped very close to the gpsA locus. Attempts at genetic complementation analysis were unsuccessful.
D002462:Cell Membrane / Q000378:metabolism; D002874:Chromosome Mapping; D003227:Conjugation, Genetic; D003433:Crosses, Genetic; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D005796:Genes; D005816:Genetic Complementation Test; D005993:Glycerolphosphate Dehydrogenase / Q000096:biosynthesis*; D005994:Glycerophosphates / Q000378:metabolism; D009154:Mutation; D010743:Phospholipids / Q000096:biosynthesis*; D011995:Recombination, Genetic; D013053:Spectrophotometry; D013237:Stereoisomerism; D013696:Temperature; D014161:Transduction, Genetic
D005994:Glycerophosphates; D010743:Phospholipids; D005993:Glycerolphosphate Dehydrogenase