Protection against gram-negative infections with antiserum to lipid A from Salmonella minnesota R595.
The ability of antisera to lipid A, induced in rabbits by immunization with lipid A complexed to various carriers, to protect mice against gram-negative infection and to inhibit the fluid loss caused by an enteropathogenic strain of Escherichia coli in the piglet ligated gut was investigated. No significant protection was obtained in either case, although passive hemolysis and quantitative precipitation tests showed the presence of antilipid A antibodies in the sera. Fluorescent antibody studies suggest that the lipid A is in a cryptic position on the surface of smooth strains of gram-negative bacteria.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D002417:Cattle / Q000276:immunology; D004927:Escherichia coli Infections / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006041:Goats / Q000276:immunology; D006386:Hemagglutination Tests; D006433:Hemocyanins; D006461:Hemolysis; D006657:Histones; D007106:Immune Sera / Q000008:administration & dosage; D007112:Immunity, Maternally-Acquired; D007115:Immunization Schedule; D007275:Injections, Intravenous; D007421:Intestine, Small / Q000276:immunology; D007422:Intestines / Q000276:immunology; D007709:Klebsiella / Q000276:immunology*; D007928:Lethal Dose 50; D008055:Lipids; D008297:Male; D008623:Mercaptoethanol; D051379:Mice; D011233:Precipitin Tests; D011817:Rabbits / Q000276:immunology; D012475:Salmonella / Q000276:immunology*; D012480:Salmonella Infections / Q000276:immunology*; D012710:Serum Albumin, Bovine; D013552:Swine
D000907:Antibodies, Bacterial; D006657:Histones; D007106:Immune Sera; D008055:Lipids; D012710:Serum Albumin, Bovine; D008623:Mercaptoethanol; D006433:Hemocyanins