Chymotryptic inhibitor I from potatoes. The amino acid sequence of subunit A.
The amino acid sequence of subunit A of the potato chymotryptic inhibitor I was determined. The sequence was deduced from analysis of fragments and peptides derived from the protein by cleavage with cyanogen bromide, N-bromosuccinimide and dilute acid, and by digestion with trypsin, thermolysin, pepsin and papain. The molecule consists of a single polypeptide chain of 84 residues, which contains two homologous regions each of 13 amino acids. The protein does not appear to be homologous with any other known proteinase inhibitors.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis*; D001966:Bromine; D002268:Carboxypeptidases; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D002918:Chymotrypsin / Q000037:antagonists & inhibitors*; D003488:Cyanogen Bromide; D003619:Dansyl Compounds; D004589:Electrophoresis, Disc; D006863:Hydrogen-Ion Concentration; D010206:Papain; D010434:Pepsin A; D010446:Peptide Fragments / Q000032:analysis; D010940:Plant Proteins / Q000032:analysis*; D013388:Succinimides; D013820:Thermolysin; D013997:Time Factors; D014357:Trypsin
D000596:Amino Acids; D003619:Dansyl Compounds; D010446:Peptide Fragments; D010940:Plant Proteins; D013388:Succinimides; D002268:Carboxypeptidases; D002918:Chymotrypsin; D014357:Trypsin; D010206:Papain; D010434:Pepsin A; D013820:Thermolysin; D003488:Cyanogen Bromide; D001966:Bromine