title: Genetic analysis of cold-sensitive ribosome maturation mutants of Escherichia coli.
abstract: Four cold-sensitive mutants of Escherichia coli, which have defects in the maturation of the 50S ribosomal subunit, were isolated. Each of the mutations was shown to map at a different locus. The loci were assigned the name rim (ribosome maturation) and were shown to map as follows: rimA is co-transduced with ilvD and with pyrE; rimB is co-transduced with aroD; conjugation experiments limited rimD to a region between ilv and malB, and conjugation experiments limited rimC to the 22 to 30 min region of the chromosome. In merodiploids heterozygous for rimA, rimB, or rimD, the wild-type allele was shown to be dominant to the mutant allele. The observation that the rim loci lie outside the strA region and separate from each other, as well as the recessive character of the rim loci, suggests that the mutants may be defective in ribosome maturation factors rather than being defective in ribosomal structural proteins.
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis; D002499:Centrifugation, Density Gradient; D002874:Chromosome Mapping; D003080:Cold Temperature; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000378:metabolism*; D005799:Genes, Dominant; D009153:Mutagens; D009154:Mutation; D009604:Nitrosoguanidines; D010761:Phosphorus Radioisotopes; D011995:Recombination, Genetic; D012270:Ribosomes / Q000378:metabolism*; D014161:Transduction, Genetic; D014316:Tritium; D014498:Uracil / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D009153:Mutagens; D009604:Nitrosoguanidines; D010761:Phosphorus Radioisotopes; D014316:Tritium; D014498:Uracil