Modulation of cell-surface antigens of a murine neuroblastoma.
Antisera were produced in rabbits to morphologically differentiated cells from the C1300 murine neuroblastoma (i.e., cells in which process formation was induced by maintenance on serum-free medium for 5 days). These antisera reacted more strongly in the complement fixation reaction with such "differentiated" cells than with "undifferentiated" (nonprocess-bearing) neuroblastoma cells. Adsorption of the antisera with undifferentiated cells removed the reactivity to cells without processes, while the reactivity with serum-free cells which possess processes was retained. Indirect immunofluorescence studies confirmed the results obtained by complement fixation and demonstrated that antibodies to the surface antigens of process-bearing cells could be adsorbed by particulate preparations from brain but not liver, spleen, or kidney. This is the first description of neural-associated cell-surface changes that correlate with the morphological differentiation in culture of neuroblastoma cells.
D000327:Adsorption; D000818:Animals; D000912:Antibodies, Neoplasm / Q000032:analysis; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D000951:Antigens, Neoplasm / Q000145:classification*; D001921:Brain / Q000276:immunology; D002454:Cell Differentiation; D002455:Cell Division; D002462:Cell Membrane / Q000276:immunology; D002999:Clone Cells; D003168:Complement Fixation Tests; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D007114:Immunization; D051379:Mice; D008858:Microscopy, Phase-Contrast; D009374:Neoplasms, Experimental / Q000276:immunology; D009447:Neuroblastoma / Q000276:immunology*; D011817:Rabbits / Q000276:immunology
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm