Fragment maps of phiX-174 replicative DNA produced by restriction enzymes from haemophilus aphirophilus and haemophilus influenzae H-I.
phiX-174 replicative form (RF) DNA was cleaved by restriction enzymes from Haemophilus aphirophilus (Hap) and H. influenzae (H-I strain Osaka) (HinH). Five fragments were produced by Hap and eight by HinH. The positions of all of the Hap fragments and six of the HinH fragments were mapped on the genome by heteroduplex transfection and DNA-DNA hybridization methods. In addition, fragment sizes of phiX-174 RF DNA and S13 RF DNA by Hap and HinH were compared. Considerable differences of the size of the fragments produced from these closely related phage genomes were observed.
D002874:Chromosome Mapping; D003090:Coliphages / Q000032:analysis*; D003433:Crosses, Genetic; D004261:DNA Replication; D004267:DNA Viruses; D004270:DNA, Circular / Q000032:analysis / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases / Q000378:metabolism*; D004926:Escherichia coli; D005796:Genes; D006190:Haemophilus / Q000201:enzymology*; D006193:Haemophilus influenzae / Q000201:enzymology; D009154:Mutation; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D010761:Phosphorus Radioisotopes; D011995:Recombination, Genetic; D013104:Spheroplasts; D013936:Thymidine; D014170:Transformation, Genetic; D014316:Tritium
D004270:DNA, Circular; D004279:DNA, Viral; D010761:Phosphorus Radioisotopes; D014316:Tritium; D004720:Endonucleases; D013936:Thymidine