title: Preparation of guanosine tetraphosphate (ppGpp) and guanosine pentaphosphate (pppGpp) from Escherichia coli ribosomes.
mesh_terms: D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D004355:Drug Stability; D004926:Escherichia coli / Q000166:cytology / Q000378:metabolism*; D005069:Evaluation Studies as Topic; D006150:Guanine Nucleotides / Q000096:biosynthesis* / Q000302:isolation & purification; D006160:Guanosine Triphosphate / Q000378:metabolism; D007553:Isotope Labeling; D008722:Methods; D010761:Phosphorus Radioisotopes; D011770:Pyruvate Kinase; D012270:Ribosomes / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D014316:Tritium
chemical_list: D006150:Guanine Nucleotides; D010761:Phosphorus Radioisotopes; D014316:Tritium; D006160:Guanosine Triphosphate; D011770:Pyruvate Kinase