title: Role of ATP in excision repair of ultraviolet radiation damage in Escherichia coli.
abstract: The effect of ATP on the first step of excision repair of ultraviolet damage in DNA has been studied using toluene-treated E. coli. During postirradiation incubation, five to six times more single-strand breaks are formed in DNA in the presence of exogenous ATP than in its absence. The ATP-dependent as well as the ATP-independent endonucleolytic activities appear to be catalyzed by the same enzyme since both activities are almost completely absent in uvrA and uvrB mutants. An ATP-dependent endonucleolytic activity has been detected in nonirradiated toluene-treated E. coli. It is concluded that ATP is required in vivo for either the incision step of repair or an enzymatic reaction preceding it.
mesh_terms: D000255:Adenosine Triphosphate / Q000502:physiology*; D002499:Centrifugation, Density Gradient; D004260:DNA Repair / Q000187:drug effects*; D004269:DNA, Bacterial / Q000528:radiation effects*; D004277:DNA, Single-Stranded / Q000528:radiation effects; D004720:Endonucleases / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008970:Molecular Weight; D009154:Mutation; D011829:Radiation Dosage; D011830:Radiation Effects; D014050:Toluene / Q000494:pharmacology; D014466:Ultraviolet Rays
chemical_list: D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D014050:Toluene; D000255:Adenosine Triphosphate; D004720:Endonucleases