Effect of peptone on Azotobacter morphology.
Pleomorphism in cultures of Azotobacter vinelandii was induced by addition of Difco peptone to the growth medium. Under the conditions of the study, 5% peptone gave rise to transient forms described as "fungoid cells" which were osmotically fragile. After some 48 hr of culture, they became osmotically stable and resumed a more typical morphology. It was shown that the pleomorphism-inducing principle in peptone was glycine.
D000596:Amino Acids / Q000032:analysis; D001322:Autoanalysis; D001395:Azotobacter / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development; D002473:Cell Wall / Q000187:drug effects; D002845:Chromatography; D003470:Culture Media; D005998:Glycine / Q000494:pharmacology; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D008970:Molecular Weight; D009995:Osmosis; D009996:Osmotic Fragility; D010461:Peptones / Q000032:analysis / Q000494:pharmacology*; D014462:Ultrafiltration
D000596:Amino Acids; D003470:Culture Media; D010461:Peptones; D005998:Glycine