Bacteriophage T7 DNA synthesis in isolated DNA-membrane complexes.
A DNA-membrane complex isolated from Escherichia coli infected with bacteriophage T7 contains newly synthesized T7 DNA and the T7 DNA polymerase (gene 5 product). The DNA present in the complex appears to exist as a concatemer which contains single-strand breaks and possibly internal single-stranded regions (gaps). The complex is capable of synthesizing T7 DNA by using endogenous template, and part of the DNA is made by a semiconservative mechanism. A portion of the in vitro synthesized DNA sediments in alkaline sucrose as 10-11S material. This DNA is converted to a larger-molecular-weight material after treatment with T4 polynucleotide ligase and E. coli DNA polymerase I.
D002250:Carbon Radioisotopes; D002462:Cell Membrane / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002623:Chemistry Techniques, Analytical; D002712:Chlorides; D003090:Coliphages / Q000378:metabolism*; D004254:DNA Nucleotidyltransferases; D004261:DNA Replication; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D004926:Escherichia coli; D007553:Isotope Labeling; D009693:Nucleic Acid Hybridization; D010761:Phosphorus Radioisotopes; D011118:Polynucleotide Ligases; D013395:Sucrose; D013698:Templates, Genetic; D014316:Tritium
D002250:Carbon Radioisotopes; D002712:Chlorides; D004279:DNA, Viral; D010761:Phosphorus Radioisotopes; D014316:Tritium; D002586:Cesium; D013395:Sucrose; D004254:DNA Nucleotidyltransferases; D011118:Polynucleotide Ligases