title: A serum bottle modification of the Hungate technique for cultivating obligate anaerobes.
abstract: The modification described utilizes a serum bottle closed with a butyl rubber stopper with a crimped metal seal for the growth of obligately anaerobic bacteria.
mesh_terms: D000693:Anaerobiosis; D001419:Bacteria / Q000254:growth & development*; D001431:Bacteriological Techniques / Q000295:instrumentation*; D001439:Bacteroides / Q000254:growth & development; D008722:Methods