title: Evaluation of the Minitek system for identification of Enterobacteriaceae.
abstract: Clinical isolates (869) and stock cultures (35) of Enterobacteriaceae were tested in parallel with the Minitek and conventional systems. The Minitek correctly identified 822 of 904 cultures. When a deoxyribonuclease plate was inoculated along with the Minitek, it was possible to speciate Enterobacteriaceae within 24 h. False-positive hydrogen sulfide reactions were the major fault with this system. Reactions were clear-cut and easy for technologists to read.
mesh_terms: D001431:Bacteriological Techniques / Q000592:standards; D003851:Deoxyribonucleases / Q000096:biosynthesis; D004755:Enterobacteriaceae / Q000145:classification* / Q000201:enzymology / Q000378:metabolism; D005069:Evaluation Studies as Topic; D005188:False Negative Reactions; D005189:False Positive Reactions; D006862:Hydrogen Sulfide / Q000096:biosynthesis; D007211:Indoles / Q000096:biosynthesis; D014508:Urea / Q000378:metabolism
chemical_list: D007211:Indoles; D014508:Urea; D003851:Deoxyribonucleases; D006862:Hydrogen Sulfide