Isolation of heterogeneous circular DNA from induced lysogens of bacteriophage Mu-1.
Covalently-closed circular DNA molecules are formed after induction of phage Mu cts4 and after infection with phage Mu cts4. The circular molecules obtained after induction have a molecular length range from 36.5 to 156.7 kilobases as measured by electron microscopic techniques. These heterogeneous molecules have no consistent correlation to exact multiples of a Mu genome equivalent (37.3 +/- 1.2 kilobases). Direct evidence is given that these molecules contain phage Mu DNA that is covalently linked to other DNA sequences.
D002499:Centrifugation, Density Gradient; D003090:Coliphages / Q000032:analysis*; D004269:DNA, Bacterial / Q000096:biosynthesis; D004270:DNA, Circular / Q000302:isolation & purification*; D004279:DNA, Viral / Q000096:biosynthesis / Q000302:isolation & purification*; D004926:Escherichia coli / Q000378:metabolism; D005838:Genotype; D006358:Hot Temperature; D008242:Lysogeny; D008854:Microscopy, Electron; D008937:Mitomycins / Q000494:pharmacology; D008970:Molecular Weight; D009154:Mutation; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009695:Nucleic Acid Renaturation
D004269:DNA, Bacterial; D004270:DNA, Circular; D004279:DNA, Viral; D008937:Mitomycins