Isolation of Acholeplasma laidlawii from commercial, serum-free tissue culture medium and studies on its survival and detection.
This report documents the first isolation of Acholeplasma laidlawii from a commercial lot of serum-free Dulbecco basal medium. Experimental studies demonstrated survival of the organism for at least 1 year depending on the concentration of the contaminating organism as well as pH and temperature of storage of the serum-free medium. A comparison of isolation methods showed that concentration by filtration through 220-nm membrane filters and testing the filters for mycoplasma recovery, especially when using phenol red-diphasic Hayflick medium, was both sensitive and practical for the average laboratory.
D000128:Acholeplasma laidlawii / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D002460:Cell Line; D002470:Cell Survival; D003470:Culture Media; D006863:Hydrogen-Ion Concentration
D003470:Culture Media