Deoxyribonucleic acid-envelope complexes isolated from Escherichia coli by free-flow electrophoresis: biochemical and electron microscope characterization.
A procedure for the preparative isolation of Escherichia coli cell wall, membrane, and deoxyribonucleic acid (DNA)-envelope complex fragments has been developed. The envelope fragments were produced by controlled mechanical cell breakage and isolated by density gradient centrifugation and subsequent preparative free-flow electrophoresis. The DNA-envelope complex fragments were shown to contain biochemical markers of both the cell wall and the membrane and by electron microscopy to be cell envelope fragments containing wall/membrane adhesion zones.
D000596:Amino Acids; D001426:Bacterial Proteins / Q000032:analysis; D001665:Binding Sites; D002250:Carbon Radioisotopes; D002458:Cell Fractionation; D002462:Cell Membrane / Q000032:analysis* / Q000201:enzymology; D002473:Cell Wall / Q000032:analysis* / Q000201:enzymology; D002499:Centrifugation, Density Gradient; D004269:DNA, Bacterial / Q000032:analysis*; D004586:Electrophoresis; D004926:Escherichia coli / Q000032:analysis / Q000166:cytology* / Q000201:enzymology; D008070:Lipopolysaccharides / Q000032:analysis; D008722:Methods; D008854:Microscopy, Electron; D011135:Polysaccharides, Bacterial / Q000032:analysis; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013936:Thymidine; D014316:Tritium
D000596:Amino Acids; D001426:Bacterial Proteins; D002250:Carbon Radioisotopes; D004269:DNA, Bacterial; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D014316:Tritium; D013385:Succinate Dehydrogenase; D013936:Thymidine