Lymphoid cells mediating tumor-specific cytotoxicity to carcinoma of the urinary bladder. Separation of the effector population using a surface marker.
Peripheral lymphocytes from patients with urinary bladder carcinoma and controls have been separated on the basis of rosette formation with sheep erythrocytes. The fractions were tested for tumor-specific cytotoxicity. The E rosette-forming cells of purity 90% respond well in PHA-induced cytotoxicity but are totally inactive in the tumor assay. The non-E rosette-forming cells (purity 91%) give enhanced activity in the tumor-specific cytotoxicity as well as in antibody-mediated target cell lysis in a model system. These data support the notion that the effector cells in cell-mediated immunity to carcinoma of the urinary bladder are members of the nonthymus-derived population of peripheral lymphocytes.
D001402:B-Lymphocytes / Q000276:immunology; D002295:Carcinoma, Transitional Cell / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D003165:Complement System Proteins; D003470:Culture Media; D003584:Cytological Techniques; D003601:Cytotoxicity Tests, Immunologic; D006801:Humans; D007104:Immune Adherence Reaction; D007111:Immunity, Cellular; D037102:Lectins; D008214:Lymphocytes / Q000276:immunology; D008545:Melanoma; D001743:Urinary Bladder; D001749:Urinary Bladder Neoplasms / Q000276:immunology*
D003470:Culture Media; D037102:Lectins; D003165:Complement System Proteins