Isolation and function of the gene A initiator of bacteriophage phi-chi 174, a highly specific DNA endonuclease.
The gene A product of Escherichia coli bacteriophage varphiX 174, necessary for initiation of varphiX DNA synthesis, has been identified and purified to near homogeneity. The gene A protein is shown to be a highly specific DNA endonuclease which breaks one phosphodiester bond in the viral strand of either double-stranded, circular varphiX replicative form I (RFI), or in single-stranded, circular varphiX viral DNA; it is inactive against all other species of DNA tested.
D002250:Carbon Radioisotopes; D002848:Chromatography, DEAE-Cellulose; D003090:Coliphages / Q000032:analysis* / Q000201:enzymology / Q000378:metabolism; D004261:DNA Replication; D004267:DNA Viruses / Q000032:analysis / Q000201:enzymology; D004270:DNA, Circular / Q000032:analysis; D004277:DNA, Single-Stranded / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis*; D003851:Deoxyribonucleases / Q000096:biosynthesis / Q000302:isolation & purification* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D005796:Genes; D005815:Genetic Code; D014764:Viral Proteins / Q000096:biosynthesis / Q000302:isolation & purification* / Q000378:metabolism; D014779:Virus Replication
D002250:Carbon Radioisotopes; D004270:DNA, Circular; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D014764:Viral Proteins; D003851:Deoxyribonucleases