Synthesis of bacteriophage M13-specific proteins in a DNA-dependent cell-free system. II. In vitro synthesis of biologically active gene 5 protein.
It is shown that gene 5 protein of bacteriophage M13 is one of the major proteins synthesized in vitro under the direction of M13 replicative-form DNA. By means of DNA-cellulose chromatography, this protein has been purified to homogeneity and its biological characteristics have been compared with those of its native counterpart. Like native gene 5 protein, the purified, in vitro-synthesized protein binds tightly and selectively to single-stranded, but not to double-stranded, DNAs. These results suggest that truly functional gene 5 protein is made in the cell-free system.
D001345:Autoradiography; D002250:Carbon Radioisotopes; D002474:Cell-Free System; D002848:Chromatography, DEAE-Cellulose; D003090:Coliphages / Q000032:analysis / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism; D004279:DNA, Viral / Q000302:isolation & purification / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D005796:Genes; D007930:Leucine / Q000378:metabolism; D011485:Protein Binding; D013462:Sulfur Radioisotopes; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism
D002250:Carbon Radioisotopes; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D013462:Sulfur Radioisotopes; D014764:Viral Proteins; D007930:Leucine