Transduction of Gal+ by coliphage T1. 3. Requirement for transcription and translation in recipient cells.
A 10- to 15-min derepression of a lambda prophage in a Gal(-) recipient during early infection with a transducing lysate of coliphage T1am will cause an increase in the efficiency of transduction of Gal(+). An increase in the efficiency of transduction occurs when the donor is either nonlysogenic or lysogenic for lambda; the increase is blocked by rifampin or chloramphenicol. With strain R901 it has been shown that efficient transduction can be blocked by treatment with rifampin after all chloramphenicol-sensitive steps have occurred.
D002701:Chloramphenicol / Q000494:pharmacology; D002876:Chromosomes, Bacterial; D003090:Coliphages / Q000187:drug effects / Q000378:metabolism*; D003470:Culture Media; D004926:Escherichia coli / Q000378:metabolism; D005690:Galactose / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000187:drug effects*; D012293:Rifampin / Q000494:pharmacology; D013386:Succinates / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects*; D014161:Transduction, Genetic / Q000187:drug effects
D003470:Culture Media; D013386:Succinates; D002701:Chloramphenicol; D012293:Rifampin; D005690:Galactose