title: Transcription of yeast DNA in vitro. Preparation of yeast DNA which is used as template by the purified DNA-dependent RNA polymerases A and B from yeast.
mesh_terms: D000255:Adenosine Triphosphate; D000645:Ammonium Sulfate; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000302:isolation & purification*; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors*; D006160:Guanosine Triphosphate; D008274:Magnesium; D008345:Manganese; D010710:Phosphates; D012313:RNA / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae; D013698:Templates, Genetic; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D010710:Phosphates; D008345:Manganese; D012313:RNA; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D004247:DNA; D012321:DNA-Directed RNA Polymerases; D008274:Magnesium; D000645:Ammonium Sulfate