Studies on the biosynthesis of alpha-putrescinylthymine in bacteriophage phi W-14-infected Pseudomonas acidovorans.
The alpha-putrescinylthymine (putThy) in bacteriophage phiW-14 DNA is synthesized at the mononucleotide level: it is labeled by uracil or deoxyuridine but not by thymidine, and it appears in the acid-soluble pool of infected cells before the onset of phage DNA synthesis. The methylene group at the C-5 position of the pyrimidine moiety of putThy is derived in vivo from a C(1) unit. Extracts of a phage infected thymidine auxotroph of the host, Pseudomonas acidovorans, apparently contain a phage-specific thymidylate synthetase and a phage-specific activity which forms 5-hydroxymethyl dUMP from N(5), N(10)-methylene-tetrahydrofolate and dUMP.
D001435:Bacteriophages / Q000254:growth & development / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002474:Cell-Free System; D002854:Chromatography, Paper; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D004926:Escherichia coli / Q000201:enzymology; D005557:Formaldehyde / Q000378:metabolism; D011549:Pseudomonas / Q000378:metabolism; D011700:Putrescine / Q000096:biosynthesis; D011743:Pyrimidines / Q000096:biosynthesis*; D012694:Serine / Q000378:metabolism; D013053:Spectrophotometry; D013763:Tetrahydrofolates / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D013940:Thymidylate Synthase / Q000378:metabolism; D013941:Thymine / Q000096:biosynthesis; D014166:Transferases / Q000378:metabolism; D014316:Tritium; D014498:Uracil / Q000378:metabolism
D002250:Carbon Radioisotopes; D004279:DNA, Viral; D011743:Pyrimidines; D013763:Tetrahydrofolates; D014316:Tritium; D005557:Formaldehyde; D012694:Serine; D014498:Uracil; D014166:Transferases; D013940:Thymidylate Synthase; D013941:Thymine; D011700:Putrescine; D013936:Thymidine