IgM-producing tumors in the BALB-c mouse: a model for B-cell maturation.
Five adjuvant induced BALB/c tumors producing IgM-McPc 1748, W 3469, TEPC 183, McPc 774, and Y 5781-were characterized morphologically by electron microscopy, analysis of the distribution of surface-bound and intracytoplasmic IgM using immunofluorescence, and by biochemical study of IgM synthesis, turnover, and secretion. The cells of different tumors appear to represent different stages in B-cell maturation when compared to normal, lipopolysaccharide-stimulated B cells. Thus, McPc 1748 tumor cells resemble 10-25-h stimulated normal B cells, 3469 cells resemble 20-35-h stimulated B cells, TEPC 183 cells resemble 45-65-h stimulated B cells, Y 5781 cells resemble 80-110-h stimulated B cells, and McPc 774 cells resemble 100-130-h stimulated B cells.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D003593:Cytoplasm / Q000276:immunology / Q000648:ultrastructure; D003609:Dactinomycin / Q000494:pharmacology; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D005643:Fucose / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D006651:Histocytochemistry; D007075:Immunoglobulin M / Q000032:analysis / Q000096:biosynthesis*; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008228:Lymphoma, Non-Hodgkin / Q000276:immunology* / Q000473:pathology; D008358:Mannose / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens; D008954:Models, Biological; D009374:Neoplasms, Experimental / Q000276:immunology; D014316:Tritium
D007075:Immunoglobulin M; D008934:Mitogens; D014316:Tritium; D003609:Dactinomycin; D005643:Fucose; D007930:Leucine; D008358:Mannose; D005690:Galactose