title: Some improved methods in P22 transduction.
abstract: Recent refinements simplify methods for P22 transduction in Salmonella and allow improved recovery of phage-free transductional clones. The methods include use of: integration- and lysis-defective phage mutants, heat-killed bacteria to eliminate free phage, direct plating of phage and bacteria, replica-plating for detection of phage content of individual clones, improved broth for phage growth, and procurement of high titer phage from P22 lysogens.
mesh_terms: D002999:Clone Cells; D003470:Culture Media; D008242:Lysogeny; D008722:Methods; D009154:Mutation; D011831:Radiation Genetics; D011995:Recombination, Genetic; D012483:Salmonella Phages; D012486:Salmonella typhimurium / Q000528:radiation effects; D014161:Transduction, Genetic; D014466:Ultraviolet Rays
chemical_list: D003470:Culture Media