Involvement of two protein factors and ATP in in vitro DNA synthesis catalyzed by DNA polymerase 3 of Escherichia coli.
The in vitro conversion of single-stranded DNA from bacteriophage fd to duplex structures depends on E. coli RNA polymerase, DNA polymerase III, riboand deoxyribonucleoside triphosphates, Mg(+2), spermidine or DNA-unwinding protein of E. coli, and two additional protein factors, referred to here as Factors I and II. These two factors are also essential for dTMP incorporation catalyzed by DNA polymerase III and dependent on poly(dA).oligo(dT) primer-template. In the latter reaction, there is an absolute dependency on ATP or dATP.
D000227:Adenine Nucleotides; D000255:Adenosine Triphosphate / Q000378:metabolism*; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D003090:Coliphages; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis* / Q000302:isolation & purification; D004926:Escherichia coli / Q000032:analysis / Q000201:enzymology*; D066298:In Vitro Techniques; D009693:Nucleic Acid Hybridization; D009711:Nucleotides / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D011119:Polynucleotides; D012367:RNA, Viral; D013698:Templates, Genetic; D013942:Thymine Nucleotides / Q000378:metabolism; D014316:Tritium
D000227:Adenine Nucleotides; D001426:Bacterial Proteins; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D009711:Nucleotides; D010761:Phosphorus Radioisotopes; D011119:Polynucleotides; D012367:RNA, Viral; D013942:Thymine Nucleotides; D014316:Tritium; D000255:Adenosine Triphosphate; D004254:DNA Nucleotidyltransferases