Identification of Salmonella with the O-1 bacteriophage.
The O-1 bacteriophage test of Cherry et al. (1954) for the presumptive identification of salmonellae in the diagnostic laboratory was investigated. A phage lysate with a titer of 10(12) plaque-forming units per ml was found to be optimal. This preparation lysed 98.2% of Salmonella strains tested, while maintaining its high specificity for salmonellae. Gram-negative organisms other than salmonellae were resistant to the O-1 phage; however, 5.9% of Escherichia coli strains tested were susceptible. The O-1 phage test is a simple, rapid, inexpensive, sensitive, and specific procedure for the identification of salmonellae in the diagnostic laboratory. A presumptive identification is obtained 1 day earlier than with conventional biochemical tests.
D001419:Bacteria; D001431:Bacteriological Techniques / Q000592:standards; D001433:Bacteriolysis; D001434:Bacteriophage Typing; D003470:Culture Media; D004926:Escherichia coli; D008242:Lysogeny; D012475:Salmonella / Q000302:isolation & purification*; D012483:Salmonella Phages / Q000254:growth & development; D013997:Time Factors
D003470:Culture Media