Utilization of two distinct modes of replication by a hybrid plasmid constructed in vitro from separate replicons.
A hybrid plasmid, pSC134, that codes for two distinct sets of replication functions has been constructed in vitro by ligation of EcoRI endonuclease-cleaved pSC101 and Col E1 plasmid replicons, and has been introduced into Escherichia coli by transformation. The replication properties of the pSC134 plasmid in DNA polymerase I-defective mutants or in the presence of chloramphenicol indicate that this hybrid plasmid can utilize the functionally distinct modes of replication specified by both of its parent replicons.
D002250:Carbon Radioisotopes; D002499:Centrifugation, Density Gradient; D002701:Chloramphenicol; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects; D004269:DNA, Bacterial / Q000096:biosynthesis; D004270:DNA, Circular / Q000096:biosynthesis; D004592:Electrophoresis, Starch Gel; D004720:Endonucleases; D004926:Escherichia coli / Q000378:metabolism*; D005111:Extrachromosomal Inheritance; D009154:Mutation; D011995:Recombination, Genetic; D013936:Thymidine / Q000378:metabolism; D014170:Transformation, Genetic
D002250:Carbon Radioisotopes; D004269:DNA, Bacterial; D004270:DNA, Circular; D002701:Chloramphenicol; D004254:DNA Nucleotidyltransferases; D004720:Endonucleases; D013936:Thymidine