Antigen-specific T-cell factor in cell cooperation: physical properties and mapping in the left-hand (K) half of H-2.
Mouse thymus cells, educated to poly(tyrosyl,glutamyl)-polyDLalanyl--polylysyl [(T,G)-A--L], release an antigen-specific factor on brief culture in vitro. The factor cooperates with bone marrow cells in the antibody response to (T,G)-A--L in irradiated recipients. Its mol wt determined from Sephadex G100 chromatography is in the region of 50,000. The factor is removed by specific antigen-coated columns, but not by anti-immunoglobulin (anti-Fab, anti-micro, anti-Fv) adsorbents. The factor is removed by alloantisera directed against the H-2 haplotype of the strain in which it is produced. Moreover, only antisera with specificity for the K side of H-2 were successful in removing the factor activity.
D000327:Adsorption; D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000917:Antibody Formation; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology; D002874:Chromosome Mapping; D005796:Genes; D008040:Genetic Linkage; D005838:Genotype; D006462:Hemolytic Plaque Technique; D006649:Histocompatibility Antigens; D007128:Immunoglobulin Fragments; D007075:Immunoglobulin M; D007518:Isoantibodies; D051379:Mice; D008815:Mice, Inbred Strains; D010455:Peptides / Q000276:immunology; D011995:Recombination, Genetic; D013601:T-Lymphocytes / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D006649:Histocompatibility Antigens; D007128:Immunoglobulin Fragments; D007075:Immunoglobulin M; D007518:Isoantibodies; D010455:Peptides