title: R-factor-mediated nuclease activity involved in thymineless elimination.
abstract: R-factor 1818 (R-1818) had no effect on the efficiency of plating of ligase-deficient phage T4 mutants on strains of Escherichia coli containing excess, normal, or defective ligase. However, if the R(+) bacterial strain that overproduced ligase was first starved of thymine, its ability to propagate ligase-deficient phage was reduced by as much as fivefold compared with the burst size on the thymine-starved R(-) strain. In contrast, it was found that after ultraviolet irradiation of the host the phage burst size was higher on the R(+) ligase overproducing strain than the R(-) derivative. The maximal level of R-factor elimination produced by thymine starvation was inversely related to the ligase level of the host. Ultraviolet irradiation did not cure the R factor from strains containing wild-type levels of ligase, but did cause elimination from strains with excess or defective ligase. The results suggest that R-1818 codes for a nuclease that is induced by thymine starvation and which, possibly in conjunction with host-mediated nucleases, is responsible for its elimination under these conditions.
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003090:Coliphages / Q000201:enzymology / Q000254:growth & development; D004267:DNA Viruses; D003851:Deoxyribonucleases / Q000378:metabolism*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism / Q000528:radiation effects; D005111:Extrachromosomal Inheritance; D008025:Ligases / Q000378:metabolism; D009154:Mutation; D011830:Radiation Effects; D013941:Thymine / Q000378:metabolism*; D014466:Ultraviolet Rays; D010948:Viral Plaque Assay
chemical_list: D000900:Anti-Bacterial Agents; D003851:Deoxyribonucleases; D008025:Ligases; D013941:Thymine