title: Heat injury and recovery of vegetative cells of Clostridium botulinum type E.
abstract: Vegetative cells of Clostridium botulinum type E (Tenno) were heated at 40.5 C in a prereduced peptone-yeast extract broth (PY). During the heating period, cell numbers remained essentially constant for 3 h as indicated by roll tube counts in PY agar (PYA); however, injury and recovery from injury were observed when the cells were enumerated using PYA containing either 0.06 or 0.07% bile salts.
mesh_terms: D000693:Anaerobiosis; D001647:Bile Acids and Salts / Q000494:pharmacology; D002021:Buffers; D002452:Cell Count; D003014:Clostridium botulinum / Q000187:drug effects / Q000254:growth & development*; D003470:Culture Media; D004352:Drug Resistance, Microbial; D006358:Hot Temperature; D010461:Peptones; D010710:Phosphates; D012703:Serotyping; D012965:Sodium Chloride / Q000494:pharmacology; D013171:Spores, Bacterial / Q000254:growth & development; D013997:Time Factors
chemical_list: D001647:Bile Acids and Salts; D002021:Buffers; D003470:Culture Media; D010461:Peptones; D010710:Phosphates; D012965:Sodium Chloride