Protein composition of the outer membrane of Salmonella typhimurium: effect of lipopolysaccharide mutations.
The protein composition of the outer membrane of Salmonella typhimurium has been analyzed by electrophoresis on slabs of sodium dodecyl sulfate-acrylamide gel. This powerful technique allows very high resolution of protein mixtures and has permitted the identification of multiple major protein components of the outer membrane; no evidence for a single major component of molecular weight 44,000 was obtained. These proteins were shown to be decreased in amount in mutants which have defective lipopolysaccharides. Mutants of an apparently new type were also found which contain decreased amounts of the proteins and the parent-like lipopolysaccharide, yet are resistant to a lipopolysaccharide-specific phage, C21. Several outer membrane proteins are insoluble in sodium dodecyl sulfate unless heated at high temperature (above 70 C). A purification procedure based on this property is tentatively suggested.
D001426:Bacterial Proteins / Q000032:analysis* / Q000302:isolation & purification; D002473:Cell Wall / Q000032:analysis*; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005944:Glucosamine / Q000032:analysis; D006539:Heptoses / Q000032:analysis; D006358:Hot Temperature; D007651:Keto Acids / Q000032:analysis; D008070:Lipopolysaccharides / Q000032:analysis*; D008970:Molecular Weight; D009154:Mutation; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D012486:Salmonella typhimurium / Q000032:analysis / Q000166:cytology*; D012995:Solubility
D001426:Bacterial Proteins; D006539:Heptoses; D007651:Keto Acids; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D005944:Glucosamine