Isolation and characterization of ultraviolet light-sensitive mutants of the blue-green alga Anacystis nidulans.
Three independently isolated ultraviolet light-sensitive (uvs) mutants of Anacystis nidulans were characterized. Strain uvs-1 was most sensitive to UV in the absence of photoreactivation. Pretreatment with caffeine suppressed the dark-survival curve of strain uvs-1, indicating the presence of excision enzymes involved in dark repair. Under "black" and "white" illumination, strain uvs-1 displays photoreactivation properties nearly comparable to wild-type culture. Mutants uvs-35 and uvs-88 appeared to have partial photorecovery capacities. Upon pretreatment with chloramphenicol, photoreactivation properties of strains uvs-1 and uvs-88 were not evident although the partial photoreactivation characteristics of strain uvs-35 remained the same. Data indicate that strains uvs-1, uvs-35, and uvs-88 are probably genetically distinct UV-sensitive mutants.
D002110:Caffeine / Q000494:pharmacology; D002470:Cell Survival; D002701:Chloramphenicol / Q000494:pharmacology; D003470:Culture Media; D000458:Cyanobacteria / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification / Q000528:radiation effects*; D004260:DNA Repair; D003624:Darkness; D008027:Light; D009154:Mutation; D010775:Photic Stimulation; D011830:Radiation Effects; D014466:Ultraviolet Rays
D003470:Culture Media; D002110:Caffeine; D002701:Chloramphenicol