Influenza A neuraminidase antibody assay with sensitized erythrocytes.
Erythrocytes sensitized with purified neuraminidase (Hong Kong) antigens were used for assay of influenza A neuraminidase antibodies. The neuraminidase indirect hemagglutination test was equal to the neuraminidase hemagglutination-inhibition (enhancement) test and appeared to be better than the neuraminidase inhibition test for detection of fourfold or greater antibody rises in paired sera from influenza patients or vaccinees. It was better than both tests for detection of neuraminidase antibody. The neuraminidase indirect hemagglutination test is simple to perform and has the advantage of direct antigen-antibody assay.
D000447:Aldehydes; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral; D003168:Complement Fixation Tests; D004912:Erythrocytes / Q000276:immunology*; D005069:Evaluation Studies as Topic; D006168:Guinea Pigs / Q000276:immunology; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006801:Humans; D007106:Immune Sera; D005779:Immunodiffusion; D007252:Influenza Vaccines; D007251:Influenza, Human / Q000276:immunology*; D008722:Methods; D009439:Neuraminidase / Q000302:isolation & purification / Q000378:metabolism; D009975:Orthomyxoviridae / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D012756:Sheep / Q000276:immunology; D013634:Tannins
D000447:Aldehydes; D000914:Antibodies, Viral; D000956:Antigens, Viral; D007106:Immune Sera; D007252:Influenza Vaccines; D013634:Tannins; D009439:Neuraminidase