title: The formation of glucosides of isoflavones and of some other phenols by rabbit liver microsomal fractions.
abstract: 1. Rabbit liver microsomal fractions in vitro effected the transfer of glucuronic acid from UDP-glucuronic acid to biochanin A, formononetin, daidzein, genistein and equol. Only monoglucuronides were formed. 2. The same isoflavones were converted into monoglucosides when UDP-[6-(3)H]glucose was substituted for UDP-glucuronic acid in the incubation medium in vitro. The glucosides were formed in much lesser yield than were the glucuronides. 3. The glucoside of genistein was identified as genistin (genistein 7-glucoside) by Sephadex chromatography and reverse isotope dilution. 4. The specificity of the glucuronyl- and glucosyl-transfer mechanisms was compared for a series of steroids and other phenols in addition to the isoflavones. It was concluded that separate transferases were responsible for the formation of the two types of glycosides.
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D005260:Female; D005419:Flavonoids / Q000378:metabolism*; D005947:Glucose; D005965:Glucuronates / Q000378:metabolism; D006027:Glycosides / Q000378:metabolism*; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism*; D011817:Rabbits; D014166:Transferases / Q000378:metabolism; D014316:Tritium; D014500:Uracil Nucleotides
chemical_list: D005419:Flavonoids; D005965:Glucuronates; D006027:Glycosides; D010636:Phenols; D014500:Uracil Nucleotides; D014316:Tritium; D014166:Transferases; D005947:Glucose