Sequential degradation of insulin by rat liver homogenates.
Insulin was incubated with rat liver homogenate in the presence of glutathione. The products formed were examined by chromatography on a Sephadex G-75 column, with 50% acetic acid as eluent. The results show that insulin is degraded by rat liver homogenates in sequential order: first, a splitting of insulin into A and B chains by glutathione-insulin transhydrogenase, followed by proteolysis of the resulting polypeptides to small molecular weight components.
D000818:Animals; D000937:Antigen-Antibody Reactions; D002850:Chromatography, Gel; D003619:Dansyl Compounds; D004492:Edetic Acid; D005033:Ethylmaleimide; D005978:Glutathione; D005998:Glycine / Q000032:analysis; D007328:Insulin / Q000032:analysis / Q000378:metabolism*; D007456:Iodine Isotopes; D008099:Liver / Q000201:enzymology*; D046911:Macromolecular Substances; D008297:Male; D010088:Oxidoreductases; D010447:Peptide Hydrolases; D011817:Rabbits / Q000276:immunology; D051381:Rats; D013447:Sulfites
D003619:Dansyl Compounds; D007328:Insulin; D007456:Iodine Isotopes; D046911:Macromolecular Substances; D013447:Sulfites; D004492:Edetic Acid; D010088:Oxidoreductases; D010447:Peptide Hydrolases; D005978:Glutathione; D005033:Ethylmaleimide; D005998:Glycine