A mass-spectrometric sequence study of the enzyme ribitol dehydrogenase from Klebsiella aerogenes.
The first detailed results of the application of a low-resolution mixture analysis approach to the sequence analysis of an enzyme, ribitol dehydrogenase, are given. Examples of the interpretation of the spectra of peptide mixtures derived from this protein are described. Evidence for new fragmentation patterns observed is reported, together with an explanation of the generation of ambiguous sequences by use of a low-specificity enzyme, thermolysin. The overall sequencing strategy evolved is assessed.
D000429:Alcohol Oxidoreductases / Q000032:analysis*; D000595:Amino Acid Sequence; D002250:Carbon Radioisotopes; D003488:Cyanogen Bromide; D007709:Klebsiella / Q000201:enzymology*; D013058:Mass Spectrometry; D008970:Molecular Weight; D010434:Pepsin A; D011487:Protein Conformation; D012266:Ribose; D013820:Thermolysin; D014357:Trypsin
D002250:Carbon Radioisotopes; D012266:Ribose; D000429:Alcohol Oxidoreductases; D014357:Trypsin; D010434:Pepsin A; D013820:Thermolysin; D003488:Cyanogen Bromide