Isolation of a new soluble antigen from the yeast phase of Histoplasma capsulatum.
A method is described by which a soluble antigen was prepared from the yeast phase of Histoplasma capsulatum. This soluble preparation had a specificity greater than that of whole-cell yeast-phase antigens. In complement fixation tests with sera from human cases of histoplasmosis, blastomycosis, and coccidioidomycosis, the soluble antigen reacted in 12.1% of 141 tests with heterologous sera, whereas conventional whole-cell yeast antigens reacted in 47.3% of 91 tests with heterologous sera. The reactivities of the two types of antigens with homologous sera were essentially the same.
D000946:Antigens, Fungal / Q000302:isolation & purification*; D001759:Blastomycosis / Q000097:blood; D003047:Coccidioidomycosis / Q000097:blood; D003168:Complement Fixation Tests; D003429:Cross Reactions; D003937:Diagnosis, Differential; D006658:Histoplasma / Q000254:growth & development / Q000276:immunology*; D006660:Histoplasmosis / Q000097:blood / Q000175:diagnosis; D006801:Humans; D007106:Immune Sera; D008722:Methods; D012995:Solubility
D000946:Antigens, Fungal; D007106:Immune Sera