Properties of the enterotoxic component in Escherichia coli enteropathogenic for swine.
The enterotoxic component in sterile syncase broth filtrates of Escherichia coli strains 340 (O9:K.:NM) and P307 (O8:K87,88a,b:H19) was studied. The enterotoxic activity in both strains was retained by an ultrafiltration membrane with a molecular weight retention of 100,000 (XM-100A) and eluted from a Sephadex G-200 column in the void volume. The enterotoxic activity in strain 340 was resistant to heating at 75 C for 30 min, but the activity in strain P307 was destroyed by heating at 60 C for 30 min. The P307 Sephadex G-200 column eluate possessing the enterotoxic activity, when desalted, contained 45.8% carbohydrate and 9.3% protein, and had an ED(50) of 2.2 mg/rabbit ileal loop. Immunodiffusion studies showed that this material contained both endotoxin and acid-polysaccharide capsular material. The enterotoxic activity was acidlabile and was destroyed by Pronase, but was resistant to trypsin and eluted as a single peak in the void volume of a 4% agarose column. The enterotoxic component could not be separated from the endotoxin; in fact, the data indicated that the two components are closely associated and that the enterotoxic activity resides in material of a protein nature.
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D002845:Chromatography; D002852:Chromatography, Ion Exchange; D004768:Enterotoxins / Q000633:toxicity; D004926:Escherichia coli; D005374:Filtration; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D005779:Immunodiffusion; D011135:Polysaccharides, Bacterial / Q000032:analysis; D011402:Pronase / Q000494:pharmacology; D011817:Rabbits; D013552:Swine; D014357:Trypsin / Q000494:pharmacology; D014462:Ultrafiltration
D001426:Bacterial Proteins; D004768:Enterotoxins; D011135:Polysaccharides, Bacterial; D014357:Trypsin; D011402:Pronase