Further studies of the chemical composition of the lipopolysaccharide of Pseudomonas aeruginosa.
1. Qualitative and quantitative analytical results for the lipopolysaccharide from acetone-dried cells of Pseudomonas aeruginosa (N.C.T.C. 1999) are presented and possible contamination of the material with nucleic acid was further examined. 2. Additional sugars detected (only in large-scale hydrolysates) were mannose and arabinose; traces of spermidine and putrescine were also found. 3. The heptose component is l-glycero-d-mannoheptose. 4. The thiobarbituric acid-positive component is a 3-deoxy-2-octulonic acid, of which only 35-40% links lipid A to the polysaccharide. This linkage is not broken by hydrolysis with acetic acid up to 0.08m. 5. Liberation of lipid A required hydrolysis with 0.1m-hydrochloric acid, which substantially degraded the polysaccharide moiety. 6. Fractions obtained from the degraded polysaccharide by high-voltage electrophoresis were examined; in these, the alanine/galactosamine molar ratio is approx. 1. 7. Hydrazinolysis of whole lipopolysaccharide showed that at least 40% of the alanine is in amide linkage, possibly with galactosamine. 8. Lipid A, solubilized by alkaline methanolysis was fractionated; most of the phosphorus of the higher-molecular-weight fractions was released as P(i) by a phosphomonoesterase. 9. Hydrazinolysis of lipid A destroyed approx. 80% of the glucosamine, and glycosidically linked glucosamine oligosaccharides could not be isolated.
D000409:Alanine / Q000032:analysis; D001089:Arabinose / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D004590:Electrophoresis, Paper; D005643:Fucose / Q000032:analysis; D005688:Galactosamine / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D006539:Heptoses / Q000032:analysis; D006595:Hexosamines / Q000032:analysis; D006834:Hydrazines; D006868:Hydrolysis; D008055:Lipids / Q000302:isolation & purification; D008070:Lipopolysaccharides / Q000032:analysis*; D008358:Mannose / Q000032:analysis; D008956:Models, Chemical; D010744:Phosphoric Monoester Hydrolases; D011135:Polysaccharides, Bacterial / Q000032:analysis; D011550:Pseudomonas aeruginosa / Q000032:analysis*; D011700:Putrescine / Q000032:analysis; D013095:Spermidine / Q000032:analysis
D006539:Heptoses; D006595:Hexosamines; D006834:Hydrazines; D008055:Lipids; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D005643:Fucose; D005688:Galactosamine; D001089:Arabinose; D010744:Phosphoric Monoester Hydrolases; D005944:Glucosamine; D000409:Alanine; D008358:Mannose; D013095:Spermidine; D011700:Putrescine