Comparison of the immunofluorescent-cell counting and plaque methods for the assay of vaccinia virus.
The immunofluorescent-cell counting method was compared with the agarand liquid-overlay plaque techniques for the assay of vaccinia virus. In addition to being as quantitative, reproducible, and simple to use as the two plaque techniques, the immunofluorescent assay method was found to be more sensitive, rapid, and specific.
D000362:Agar; D000818:Animals; D000917:Antibody Formation; D000956:Antigens, Viral / Q000032:analysis; D002452:Cell Count; D002460:Cell Line; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique / Q000592:standards*; D000882:Haplorhini; D006367:HeLa Cells; D006462:Hemolytic Plaque Technique; D006801:Humans; D007106:Immune Sera; D007668:Kidney; D008722:Methods; D011817:Rabbits / Q000276:immunology; D014616:Vaccinia virus / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification; D014776:Virus Cultivation
D000956:Antigens, Viral; D007106:Immune Sera; D000362:Agar