Mode of action of phleomycin on Bacillus subtilis.
Phleomycin appears to act on the cell wall and membrane to induce the release of membrane-associated deoxyribonucleic acid (DNA) and the degradation of the DNA. Degradation occurs in a series of energy-requiring endonuclease and exonuclease reactions. These produce, first, single-strand breaks, then double-strand breaks, and finally almost complete solubilization of the cellular DNA. The in vivo inhibition of DNA synthesis by phleomycin is probably a secondary effect caused by the destruction of template DNA.
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis; D002247:Carbon Isotopes; D002462:Cell Membrane / Q000187:drug effects; D002473:Cell Wall / Q000187:drug effects; D002499:Centrifugation, Density Gradient; D004269:DNA, Bacterial / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism; D003851:Deoxyribonucleases; D007930:Leucine / Q000378:metabolism; D009691:Nucleic Acid Denaturation; D010455:Peptides / Q000494:pharmacology; D012329:RNA, Bacterial / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D012997:Solvents; D013698:Templates, Genetic / Q000187:drug effects; D013936:Thymidine / Q000378:metabolism; D014050:Toluene; D014238:Trichloroacetic Acid; D014316:Tritium; D014498:Uracil / Q000378:metabolism
D000903:Antibiotics, Antineoplastic; D001426:Bacterial Proteins; D002247:Carbon Isotopes; D004269:DNA, Bacterial; D010455:Peptides; D012329:RNA, Bacterial; D012997:Solvents; D014316:Tritium; D014050:Toluene; D014498:Uracil; D014238:Trichloroacetic Acid; D003851:Deoxyribonucleases; D007930:Leucine; D013936:Thymidine