Synthesis of branced-chain aminoacyl-transfer ribonucleid acid synthetases in a Salmonella typhimurium mutant with an altered biosynthetic L-threonine deaminase.
The differential rates of synthesis of the three branched-chain aminoacyl-transfer ribonucleic acid synthetases were measured in Salmonella typhimurium LT-2 and a mutant, ilvA504. The mutant produced an l-threonine deaminase with a decreased affinity for its cofactor, pyridoxal-5'-monophosphate. The addition of pyridoxal-5'-monophosphate to cultures of strain ilvA504 growing in excess isoleucine, valine, and leucine resulted in an increased rate of growth and repression of the synthesis of the isoleucine and valine biosynthetic enzymes. No differences in the rate of synthesis of the branched-chain aminoacyl-transfer ribonucleic acid synthetases were observed in cultures of ilvA504 growing with or without added pyridoxal-5'-monophosphate. The differential rates of synthesis of all three enzymes were similar to the rates measured in strain LT-2. These experiments suggest that different forms of the ilvA gene product are involved in the regulation of the branched-chain amino acid biosynthetic enzymes and the branched-chain aminoacyl-transfer ribonucleic acid synthetases.
D000604:Amino Acyl-tRNA Synthetases / Q000096:biosynthesis*; D002250:Carbon Radioisotopes; D003470:Culture Media; D006836:Hydro-Lyases / Q000378:metabolism*; D007532:Isoleucine / Q000378:metabolism; D007700:Kinetics; D009154:Mutation; D011732:Pyridoxal Phosphate / Q000378:metabolism; D012486:Salmonella typhimurium / Q000201:enzymology*; D013912:Threonine; D014316:Tritium; D014633:Valine / Q000378:metabolism
D002250:Carbon Radioisotopes; D003470:Culture Media; D007532:Isoleucine; D014316:Tritium; D013912:Threonine; D011732:Pyridoxal Phosphate; D006836:Hydro-Lyases; D000604:Amino Acyl-tRNA Synthetases; D014633:Valine