Experience with microbiological assay for folate using a chloramphenicol-resistant L. casei strain.
Experience with a semi-automated L. casei folate assay using a chloramphenicol-resistant strain of organism is described. Control of pH of medium and incubation time was required. The normal range for serum folate was 2 to 16 ng/ml and red cell folate 200 to 600 ng/ml.
D001681:Biological Assay; D002701:Chloramphenicol; D004352:Drug Resistance, Microbial; D004912:Erythrocytes / Q000032:analysis; D005492:Folic Acid / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus; D007780:Lacticaseibacillus casei; D008722:Methods; D013997:Time Factors
D002701:Chloramphenicol; D005492:Folic Acid