title: Template specificity of ribonucleic acid polymerase in asporogenous mutants of Bacillus subtilis.
abstract: Asporogenous mutants of Bacillus subtilis were examined for the change in template specificity of ribonucleic acid (RNA) polymerase characteristic of wild-type cells undergoing sporulation. Mutants blocked at stages II, III, and IV showed a changed specificity of the enzyme after the end of growth and were in this respect indistinguishable from the wild type. The RNA polymerase of eight stage-zero mutants (out of nine tested) which possess mutations that map at six distinct loci retained the template specificity of vegetative cells.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001412:Bacillus subtilis / Q000201:enzymology* / Q000254:growth & development; D002474:Cell-Free System; D002874:Chromosome Mapping; D003090:Coliphages; D004279:DNA, Viral; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005827:Genetics, Microbial; D009154:Mutation; D011119:Polynucleotides; D013171:Spores, Bacterial / Q000254:growth & development; D013698:Templates, Genetic; D013997:Time Factors; D014158:Transcription, Genetic; D014316:Tritium; D014500:Uracil Nucleotides / Q000378:metabolism
chemical_list: D004279:DNA, Viral; D011119:Polynucleotides; D014500:Uracil Nucleotides; D014316:Tritium; D000255:Adenosine Triphosphate; D012321:DNA-Directed RNA Polymerases