Comparative characteristics of human and porcine staphylococci and their differentiation in burn xenografting procedures.
Staphylococcus epidermidis from porcine skin differed from human cutaneous S. epidermidis in that the former strains were principally of the Baird-Parker biotype III group. The porcine-type strains were more proteolytic on casein and gelatin than were human strains, which were primarily of biotype II. Porcine strains were also elastolytic. Using supernatant fluids of broth cultures, the biotype II strains, but not the type III strains, were lipolytic in action on triolein. Both types of staphylococci were similar in enzymatic activities on Tween 80, egg yolk, and tributyrin. Elastase activity was not found in broth supernatant fluid of these bacteria. The porcine strains were retarded or inhibited from growing in media at pH 5.5. Action on casein agar followed by demonstration of elastase activity were used as markers to detect the porcine S. epidermidis strains in xenografts and on human burn wound grafting sites.
D000328:Adult; D000818:Animals; D001431:Bacteriological Techniques; D002056:Burns / Q000382:microbiology* / Q000601:surgery; D002364:Caseins / Q000378:metabolism; D002648:Child; D003470:Culture Media; D005780:Gelatin / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010196:Pancreatic Elastase / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification; D012703:Serotyping; D012867:Skin / Q000382:microbiology; D016038:Skin Transplantation; D013045:Species Specificity; D013210:Staphylococcus / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D013501:Surface-Active Agents / Q000378:metabolism; D013552:Swine; D014183:Transplantation, Heterologous; D014304:Triolein / Q000378:metabolism
D002364:Caseins; D003470:Culture Media; D013501:Surface-Active Agents; D014304:Triolein; D005780:Gelatin; D010196:Pancreatic Elastase