Rabies antibody determination by immunofluorescence in tissue culture.
A tissue culture (TC)-fluorescent antibody (FA) technique for the measurement of rabies-neutralizing antibody was found to be reliable and comparable to the standard mouse serum neutralization test. This test was performed with BHK-21 cells infected with the ERA vaccine virus strain on Lab-Tek TC chamber slides. A Flury high egg passage (HEP) rabies virus strain grown on continuous line of African green monkey (Vero) and on BHK-21 cells was investigated to determine its utilization in a TC-FA neutralization procedure. Although both the HEP and ERA viruses infected Vero and BHK-21 cells, the amount of fluorescent antigen observed was most consistent with ERA virus and BHK-21 cells.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002460:Cell Line; D006224:Cricetinae; D046508:Culture Techniques; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D000882:Haplorhini; D007668:Kidney; D007928:Lethal Dose 50; D051379:Mice / Q000276:immunology; D009500:Neutralization Tests; D011818:Rabies / Q000175:diagnosis* / Q000276:immunology; D011820:Rabies virus / Q000276:immunology*; D012698:Serologic Tests
D000914:Antibodies, Viral