Rapid preparation of hemagglutinins of togaviruses from infected cell culture fluids.
Antigens in infected cell culture fluids can be easily concentrated by polyethylene glycol precipitation to yield suitable hemagglutinating and complement-fixing antigens for several togaviruses.
D000818:Animals; D001102:Arbovirus Infections / Q000276:immunology; D001103:Arboviruses / Q000276:immunology*; D001921:Brain / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured / Q000382:microbiology; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D003168:Complement Fixation Tests; D003470:Culture Media; D003588:Cytopathogenic Effect, Viral; D004586:Electrophoresis; D006018:Glycols; D000882:Haplorhini; D006385:Hemagglutination Inhibition Tests; D006389:Hemagglutinins, Viral / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007668:Kidney; D007930:Leucine; D008722:Methods; D051379:Mice; D010759:Phosphorus Isotopes; D013045:Species Specificity; D014316:Tritium; D014443:Tyrosine; D014776:Virus Cultivation
D003470:Culture Media; D006018:Glycols; D006389:Hemagglutinins, Viral; D010759:Phosphorus Isotopes; D014316:Tritium; D014443:Tyrosine; D007930:Leucine