Indirect hemagglutination test for chlamydial antibodies.
An indirect hemagglutination (IHA) test is described for chlamydial antibodies in psittacosis diagnostic sera; for this test tanned sheep erythrocytes sensitized with a deoxycholate extract of Chlamydia psittaci grown in Vero cell monolayers were used. Adaptation of the IHA test to the Microtiter system decreased sensitivity; nevertheless, the Microtiter-IHA test was more sensitive than the complement fixation test. Lymphogranuloma venereum antibodies also were detected by using antigen extracted from C. psittaci.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000942:Antigens, Bacterial; D002460:Cell Line; D002689:Chlamydia / Q000254:growth & development / Q000276:immunology*; D003168:Complement Fixation Tests; D003937:Diagnosis, Differential; D004912:Erythrocytes / Q000276:immunology; D005069:Evaluation Studies as Topic; D006168:Guinea Pigs / Q000276:immunology; D000882:Haplorhini; D006386:Hemagglutination Tests; D006801:Humans; D007106:Immune Sera; D007668:Kidney; D008219:Lymphogranuloma Venereum / Q000175:diagnosis; D008722:Methods; D009956:Psittacosis / Q000175:diagnosis*; D011817:Rabbits / Q000276:immunology; D012756:Sheep / Q000276:immunology; D013634:Tannins
D000906:Antibodies; D000942:Antigens, Bacterial; D007106:Immune Sera; D013634:Tannins