title: Specificity and kinetics of triose phosphate isomerase from chicken muscle.
abstract: The isolation of crystalline triose phosphate isomerase from chicken breast muscle is described. The values of k(cat.) and K(m) for the reaction in each direction were determined from experiments over wide substrate-concentration ranges, and the reactions were shown to obey simple Michaelis-Menten kinetics. With d-glyceraldehyde 3-phosphate as substrate, k(cat.) is 2.56x10(5)min(-1) and K(m) is 0.47mm; with dihydroxyacetone phosphate as substrate, k(cat.) is 2.59x10(4)min(-1) and K(m) is 0.97mm. The enzyme-catalysed exchange of the methyl hydrogen atoms of the ;virtual substrate' monohydroxyacetone phosphate with solvent (2)H(2)O or (3)H(2)O was shown. This exchange is about 10(4)-fold slower than the corresponding exchange of the C-3 hydrogen of dihydroxyacetone phosphate. The other deoxy substrate, 3-hydroxypropionaldehyde phosphate, was synthesized, but is too unstable in aqueous solution for analogous proton-exchange reactions to be studied.
mesh_terms: D000096:Acetone; D000447:Aldehydes; D000818:Animals; D002238:Carbohydrate Epimerases / Q000302:isolation & purification / Q000378:metabolism*; D002645:Chickens; D002848:Chromatography, DEAE-Cellulose; D003460:Crystallization; D003903:Deuterium; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008970:Molecular Weight; D009132:Muscles / Q000201:enzymology*; D009943:Organophosphorus Compounds; D011522:Protons; D013438:Sulfhydryl Compounds; D014306:Trioses; D014316:Tritium
chemical_list: D000447:Aldehydes; D009943:Organophosphorus Compounds; D011522:Protons; D013438:Sulfhydryl Compounds; D014306:Trioses; D014316:Tritium; D000096:Acetone; D003903:Deuterium; D002238:Carbohydrate Epimerases