Spermine synthesis in the uterus of the ovariectomized rat in response to oestradiol-17 .
We reported that spermidine and spermine pools in the uterus both doubled within 24h after oestradiol administration to castrated rats (Russell & Taylor, 1971). Now we have studied the enzymic synthesis of spermine (by spermidine-dependent S-adenosyl-l-methionine decarboxylase) and find that the activity of the enzyme(s) involved is elevated soon after hormone administration. Enzyme activity is increased within 4h and is five times that of controls within 24h. Cycloheximide or actinomycin D administered at the time of oestradiol injection completely blocked the increase in enzyme activity. The enzyme involved in spermine synthesis, S-adenosyl-l-methionine decarboxylase, with S-adenosyl-l-methionine and spermidine as required substrates, was partially purified on Sephadex and DEAE-cellulose columns. The decarboxylation of S-adenosyl-l-methionine could not be separated from the transfer of a propylamine moiety from the decarboxylated S-adenosyl-l-methionine to spermidine to form spermine. We were unable also to separate this system from the enzyme that formed spermidine when S-adenosyl-l-methionine and putrescine are used as substrates. Spermidine-stimulated S-adenosyl-l-methionine decarboxylase has an apparent half-life of 60min, identical with the half-life reported for putrescine-stimulated S-adenosyl-l-methionine decarboxylase. These results strongly suggest that the same enzyme(s) operate in the synthesis of both spermidine and spermine.
D000818:Animals; D002245:Carbon Dioxide; D002247:Carbon Isotopes; D002262:Carboxy-Lyases; D002369:Castration; D002848:Chromatography, DEAE-Cellulose; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology*; D004965:Estrogen Antagonists; D005260:Female; D011437:Propylamines; D011700:Putrescine / Q000494:pharmacology; D051381:Rats; D012436:S-Adenosylmethionine; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000096:biosynthesis*; D014599:Uterus / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*
D002247:Carbon Isotopes; D004965:Estrogen Antagonists; D011437:Propylamines; D002245:Carbon Dioxide; D003609:Dactinomycin; D013096:Spermine; D004958:Estradiol; D012436:S-Adenosylmethionine; D003513:Cycloheximide; D002262:Carboxy-Lyases; D013095:Spermidine; D011700:Putrescine