Nature of ribonucleic acid synthesis during early sporulation in Saccharomyces cerevisiae.
Phosphate uptake in sporulating cultures of Saccharomyces cerevisiae has been found to occur approximately 2 h after the transfer to sporulation medium. Early ribonucleic acid synthesis begins at approximately 4 h and continues to 8 h. Incorporation of phosphate into acid-extractable precursor pools parallels phosphate uptake. In triple-labeling experiments it was observed that the breakdown of vegetatively synthesized ribonucleic acid is not a significant source of precursors for ribonucleic acid synthesis during sporulation. The majority of the ribonucleic acid made in a 10-min period during sporulation does not migrate on gels with precursor or mature ribosomal ribonucleic acid.
D000225:Adenine / Q000378:metabolism; D002250:Carbon Radioisotopes; D004591:Electrophoresis, Polyacrylamide Gel; D007553:Isotope Labeling; D010710:Phosphates / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D012313:RNA / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D012335:RNA, Ribosomal / Q000378:metabolism; D012343:RNA, Transfer / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013172:Spores, Fungal / Q000378:metabolism*; D013997:Time Factors; D014316:Tritium
D002250:Carbon Radioisotopes; D010710:Phosphates; D010761:Phosphorus Radioisotopes; D012335:RNA, Ribosomal; D014316:Tritium; D012313:RNA; D012343:RNA, Transfer; D000225:Adenine