Minimal requirements for commitment to sporulation in Bacillus megaterium.
Commitment to sporulation was examined by means of both endotrophic sporulation and rejuvenation experiments. In both cases, a point of commitment to the completion of sporulation occurred at stage II. With 2 x 10(8) cells/ml, the process by which commitment occurred required 0.5 mm Mg(2+), 10.0 mm phosphate, and an energy source (minimum of 5.0 mm acetate). For completion of sporulation and formation of normal, heat-resistant refractile spores, 0.3 mm Ca(2+) was required. Except for this Ca(2+) requirement, the completion of sporulation after commitment was independent of the nature of the surrounding medium. Some aspects of the utilization of these required substances were examined.
D000085:Acetates / Q000378:metabolism; D001410:Bacillus megaterium / Q000254:growth & development* / Q000378:metabolism; D002021:Buffers; D002118:Calcium / Q000378:metabolism; D002247:Carbon Isotopes; D003470:Culture Media; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D013053:Spectrophotometry; D013170:Spores / Q000254:growth & development*; D013171:Spores, Bacterial / Q000254:growth & development / Q000378:metabolism
D000085:Acetates; D002021:Buffers; D002247:Carbon Isotopes; D003470:Culture Media; D010710:Phosphates; D008274:Magnesium; D002118:Calcium