Spore pool glutamic acid as a metabolite in germination.
Spore glutamic acid pools were examined in dormant and germinating spores using colorimetric and (14)C analytical procedures. Germination of spores of Bacillus megaterium (parent strain), initiated by d-glucose, was accompanied by a rapid drop in the level of spore pool glutamate, from 12.0 mug/mg of dry spores to 7.7 mug/mg of dry spores after 30 sec of germination. Similar decreases in extractable spore pool glutamate were observed with l-alanine-initiated germination of B. licheniformis spores. On the other hand, glutamate pools of mutant spores of B. megaterium, with a requirement of gamma-aminobutyric acid for spore germination, remained unchanged for 9 min of germination, at which time more than 50% of the spore population had germinated. Evidence for conversion of spore pool glutamate to gamma-aminobutyric acid during germination of spores of B. megaterium (parent strain) was obtained.
D000409:Alanine / Q000378:metabolism; D000613:Aminobutyrates / Q000378:metabolism; D001407:Bacillus / Q000254:growth & development* / Q000378:metabolism; D001410:Bacillus megaterium / Q000254:growth & development* / Q000378:metabolism; D002247:Carbon Isotopes; D002845:Chromatography; D003124:Colorimetry; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism*; D013053:Spectrophotometry; D013170:Spores / Q000254:growth & development*; D013171:Spores, Bacterial / Q000254:growth & development / Q000378:metabolism; D013237:Stereoisomerism; D013997:Time Factors
D000613:Aminobutyrates; D002247:Carbon Isotopes; D003470:Culture Media; D005971:Glutamates; D005947:Glucose; D000409:Alanine