title: Rapid preparation of hemagglutinins of togaviruses from infected cell culture fluids.
abstract: Antigens in infected cell culture fluids can be easily concentrated by polyethylene glycol precipitation to yield suitable hemagglutinating and complement-fixing antigens for several togaviruses.
mesh_terms: D000818:Animals; D001102:Arbovirus Infections / Q000276:immunology; D001103:Arboviruses / Q000276:immunology*; D001921:Brain / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured / Q000382:microbiology; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D003168:Complement Fixation Tests; D003470:Culture Media; D003588:Cytopathogenic Effect, Viral; D004586:Electrophoresis; D006018:Glycols; D000882:Haplorhini; D006385:Hemagglutination Inhibition Tests; D006389:Hemagglutinins, Viral / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007668:Kidney; D007930:Leucine; D008722:Methods; D051379:Mice; D010759:Phosphorus Isotopes; D013045:Species Specificity; D014316:Tritium; D014443:Tyrosine; D014776:Virus Cultivation
chemical_list: D003470:Culture Media; D006018:Glycols; D006389:Hemagglutinins, Viral; D010759:Phosphorus Isotopes; D014316:Tritium; D014443:Tyrosine; D007930:Leucine