Microtubule formation in vitro in solutions containing low calcium concentrations.
Isolated rat brain tubulin can be repolymerized in vitro in solutions containing adenosine triphosphate or guanosine triphosphate, magnesium ions, and a good calcium chelator. The extreme sensitivity of tubulin to calcium ions explains the failure of previous efforts to obtain polymerization and suggests a possible mechanism for regulation of microtubule polymerization in vivo.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000378:metabolism; D002118:Calcium / Q000494:pharmacology*; D002122:Calcium Chloride / Q000494:pharmacology; D002614:Chelating Agents / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D006160:Guanosine Triphosphate / Q000494:pharmacology; D066298:In Vitro Techniques; D008278:Magnesium Sulfate / Q000494:pharmacology; D008870:Microtubules / Q000378:metabolism*; D011108:Polymers; D051381:Rats; D014020:Tissue Extracts / Q000378:metabolism
D002614:Chelating Agents; D011108:Polymers; D014020:Tissue Extracts; D008278:Magnesium Sulfate; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D004492:Edetic Acid; D002122:Calcium Chloride; D002118:Calcium