A simple lipase assay using trichloroacetic acid.
An extremely rapid and sensitive assay for lipoprotein lipase activity, suitable for routine determinations, is described. The substrate for the assay is emulsified [2-(3)H]glyceryl trioleate, activated by serum. The method is based on trichloroacetic acid precipitation of unreacted substrate and measurement of (3)H-labeled glycerol.
D000273:Adipose Tissue / Q000201:enzymology; D000818:Animals; D011232:Chemical Precipitation; D005990:Glycerol; D006651:Histocytochemistry; D066298:In Vitro Techniques; D008071:Lipoprotein Lipase / Q000032:analysis* / Q000097:blood; D008722:Methods; D009829:Oleic Acids; D051381:Rats; D014238:Trichloroacetic Acid; D014316:Tritium
D009829:Oleic Acids; D014316:Tritium; D014238:Trichloroacetic Acid; D008071:Lipoprotein Lipase; D005990:Glycerol