Improvement of microbial assays of vitamins.
A method for the improvement of microbial assays of vitamins, which involves the addition of a surfactant to the incubated test, was developed. This surfactant tends to eliminate bacterial clumping, giving a uniform suspension of single cells, thereby making the turbidity readings less erratic and the actual assay standard curves more closely related to the desired theoretical curve.
D001431:Bacteriological Techniques; D001681:Biological Assay; D013293:Enterococcus faecalis / Q000187:drug effects / Q000254:growth & development; D005492:Folic Acid / Q000032:analysis; D007294:Inositol / Q000032:analysis; D002955:Leucovorin / Q000032:analysis; D008239:Lysine / Q000032:analysis; D008722:Methods; D009539:Nicotinic Acids / Q000032:analysis; D010205:Pantothenic Acid / Q000032:analysis; D011736:Pyridoxine / Q000032:analysis; D013501:Surface-Active Agents / Q000494:pharmacology; D014805:Vitamin B 12 / Q000032:analysis; D014815:Vitamins / Q000032:analysis*
D009539:Nicotinic Acids; D013501:Surface-Active Agents; D014815:Vitamins; D010205:Pantothenic Acid; D007294:Inositol; D005492:Folic Acid; D008239:Lysine; D011736:Pyridoxine; D014805:Vitamin B 12; D002955:Leucovorin