In vitro repair of x-irradiated DNA extracted from Bacillus subtilis deficient in polymerase I.
DNA extracted from x-irradiated cells of a mutant of Bacillus subtilis deficient in DNA polymerase I has greatly reduced biological activity. This DNA, which has many single-strand nicks, can be repaired in vitro with purified DNA polymerase I and DNA ligase or with lysates of wild-type B. subtilis cells, which restore biological activity and increase the single-strand molecular weight. Lysates of polymerase I-deficient B. subtilis cells cannot repair such irradiated DNA until purified DNA polymerase I or lysate from wild-type cells is added to the deficient lysate.
D001412:Bacillus subtilis / Q000201:enzymology* / Q000528:radiation effects; D002499:Centrifugation, Density Gradient; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004260:DNA Repair; D004269:DNA, Bacterial / Q000032:analysis / Q000096:biosynthesis* / Q000528:radiation effects; D004926:Escherichia coli / Q000201:enzymology; D008970:Molecular Weight; D009154:Mutation; D010759:Phosphorus Isotopes; D011118:Polynucleotide Ligases / Q000378:metabolism; D014316:Tritium
D004269:DNA, Bacterial; D010759:Phosphorus Isotopes; D014316:Tritium; D004254:DNA Nucleotidyltransferases; D011118:Polynucleotide Ligases