Some unique properties of the deoxyribonucleic acid-bearing portion of the bacterial membrane.
By using the M-band technique we have shown that portions of the membranes of Bacillus megaterium and Escherichia coli vary in their affinity for magnesium-Sarkosyl crystals and in phospholipid composition. The portion to which deoxyribonucleic acid is attached comprises as little as 4% of the total cell membrane, has a particularly high degree of affinity for magnesium-Sarkosyl crystals, and is rich in phosphatidylethanolamine. The M-band fractionation does not depend on the use of lysozyme.
D001410:Bacillus megaterium / Q000032:analysis / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis; D002247:Carbon Isotopes; D002308:Cardiolipins / Q000032:analysis; D002458:Cell Fractionation; D002462:Cell Membrane / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D002845:Chromatography; D004269:DNA, Bacterial / Q000032:analysis / Q000378:metabolism*; D004926:Escherichia coli / Q000032:analysis / Q000378:metabolism; D005990:Glycerol; D008274:Magnesium / Q000494:pharmacology; D009113:Muramidase; D010710:Phosphates; D010714:Phosphatidylethanolamines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D010759:Phosphorus Isotopes; D011523:Protoplasts / Q000187:drug effects; D013501:Surface-Active Agents / Q000378:metabolism; D014316:Tritium
D001426:Bacterial Proteins; D002247:Carbon Isotopes; D002308:Cardiolipins; D004269:DNA, Bacterial; D010710:Phosphates; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D010759:Phosphorus Isotopes; D013501:Surface-Active Agents; D014316:Tritium; D009113:Muramidase; D008274:Magnesium; D005990:Glycerol