Use of a clonal line of porcine kidney cell cultures for primary isolation and vaccine studies with adenoviruses.
The clonal line (Y15) of porcine kidney stable cells provided a recovery system for adenovirus T4 from specimens from adults with respiratory illnesses that was as sensitive as human embryo kidney cultures. Adenoviruses T7 from adults, and T1, 2, 3, and 5 from children could be readily isolated in porcine kidney cell cultures. The latter were useful for adenovirus vaccine studies in that infectivity titers of live virus vaccine and neutralization antibody responses after vaccination were equal to those obtained in human embryo kidney cultures.
D000256:Adenoviridae / Q000276:immunology / Q000302:isolation & purification*; D000328:Adult; D000818:Animals; D002277:Carcinoma; D002460:Cell Line; D002648:Child; D002999:Clone Cells; D000882:Haplorhini; D006801:Humans; D007668:Kidney / Q000196:embryology; D007822:Laryngeal Neoplasms; D008168:Lung; D009062:Mouth Neoplasms; D009500:Neutralization Tests; D010614:Pharynx / Q000382:microbiology; D012007:Rectum / Q000382:microbiology; D012703:Serotyping; D013552:Swine; D014765:Viral Vaccines
D014765:Viral Vaccines