Control of fatty acid synthesis in bacteria.
When glycerol-requiring auxotrophs of Bacillus subtilis are deprived of glycerol, the synthesis of fatty acids continues at an apparent rate of 20 to 50% that of supplemented cultures. The newly synthesized fatty acids are not incorporated into phospholipid and accumulate as free fatty acids. These molecules undergo a much more rapid turnover than phospholipid fatty acids, and the rate of turnover is sufficient to indicate that the rate of fatty acid synthesis in glycerol-deprived cultures is similar to that in supplemented ones. The average chain length of the free fatty acids is greater than that of the phospholipid fatty acids. Cells deprived of required amino acids also show a diminution in the apparent rate of fatty acid synthesis; however, in this case, the fatty acids accumulate in phospholipid, and no increase of the free fatty acid fraction is observed. It is argued on the basis of these findings that the control of lipid synthesis does not operate at the level of transacylation but must act on one or more of the reactions of the fatty acid synthetase.
D000085:Acetates / Q000378:metabolism; D000596:Amino Acids; D001345:Autoradiography; D001412:Bacillus subtilis / Q000254:growth & development / Q000378:metabolism*; D002247:Carbon Isotopes; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D003470:Culture Media; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D064429:Fatty Acid Synthases / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis* / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000096:biosynthesis; D005827:Genetics, Microbial; D005990:Glycerol; D007700:Kinetics; D009154:Mutation; D010743:Phospholipids / Q000096:biosynthesis
D000085:Acetates; D000596:Amino Acids; D002247:Carbon Isotopes; D003470:Culture Media; D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified; D010743:Phospholipids; D064429:Fatty Acid Synthases; D005990:Glycerol