Comparison of the antigenicity of phenol and ethylenediaminetetraacetate complexes isolated from cell walls of Salmonella enteritidis.
Cell walls of Salmonella enteritidis were extracted with phenol and with ethylenediaminetetraacetate (EDTA). Phenol extracts were consistently more antigenic in chickens than EDTA extracts. Differences in the action of EDTA and phenol upon bacterial cell walls were demonstrated by the use of radioisotopes.
D000372:Agglutination Tests; D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000302:isolation & purification*; D001224:Aspartic Acid / Q000302:isolation & purification; D002247:Carbon Isotopes; D002473:Cell Wall / Q000187:drug effects / Q000276:immunology*; D002645:Chickens; D004492:Edetic Acid / Q000494:pharmacology; D010636:Phenols / Q000494:pharmacology; D012475:Salmonella / Q000276:immunology*; D012477:Salmonella enteritidis / Q000166:cytology / Q000276:immunology; D012995:Solubility; D015032:Zinc / Q000302:isolation & purification; D015033:Zinc Isotopes
D000941:Antigens; D002247:Carbon Isotopes; D010636:Phenols; D015033:Zinc Isotopes; D001224:Aspartic Acid; D004492:Edetic Acid; D015032:Zinc