Metabolism of gentiobiose in Aerobacter aerogenes.
Cleavage of gentiobiose in cell extracts of gentiobiose-grown Aerobacter aerogenes was dependent on the presence of adenosine 5'-triphosphate (ATP). The enzymes that participate in the overall reaction were shown to be a beta-glucoside kinase, which catalyzes the phosphorylation of gentiobiose with ATP to form gentiobiose monophosphate [6-O-phosphoryl-beta-d-glucopyranosyl-(1 --> 6)-d-glucose], and a phospho-beta-glucosidase, which catalyzes the hydrolytic cleavage of gentiobiose monophosphate to form equimolar amounts of d-glucose and d-glucose 6-phosphate. Although the beta-glucoside kinase was previously shown to catalyze the phosphorylation of many beta-glucosides that serve as growth substrates (i.e., gentiobiose, cellobiose, cellobiitol, salicin, arbutin, methyl beta-d-glucoside, and phenyl beta-d-glucoside), mutant analysis and induction studies indicate that it functions only in the metabolism of gentiobiose, cellobiose, and cellobiitol.
D000255:Adenosine Triphosphate / Q000378:metabolism; D002474:Cell-Free System; D055598:Chemical Phenomena; D002621:Chemistry; D002854:Chromatography, Paper; D004187:Disaccharides / Q000378:metabolism*; D004754:Enterobacter / Q000201:enzymology / Q000378:metabolism*; D004790:Enzyme Induction; D005947:Glucose / Q000096:biosynthesis; D005958:Glucosephosphates / Q000096:biosynthesis; D005959:Glucosidases / Q000378:metabolism; D006027:Glycosides / Q000096:biosynthesis / Q000378:metabolism; D006868:Hydrolysis; D009154:Mutation; D010085:Oxidative Phosphorylation; D010770:Phosphotransferases / Q000378:metabolism; D013053:Spectrophotometry; D013237:Stereoisomerism
D004187:Disaccharides; D005958:Glucosephosphates; D006027:Glycosides; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D005959:Glucosidases; D005947:Glucose