[Determination of bacteria count by enumeration of microcolonies with an electronic counter apparatus (author's transl)].
In this paper a method is described to determine the bacteria count of samples by enumerating the microcolonies with the aid of a "Coulter Counter" automatic apparatus. Samples of bacterial cultures were incubated in a suitable medium at 32 degrees C for 12 h, then passed through a Coulter Counter aperture of 200 mu diameter to count the microcolonies. Microcolonies from cultures grown in similar conditions were also enumerated by the classical plate method. Comparison showed a good correction between the two sets of results after logarithmic transformation. Some advantages and limitations of this automatic technique are discussed, in view of its routine employment in food analysis.
D001431:Bacteriological Techniques / Q000379:methods*; D004582:Electronics, Medical
Determinazione della carica microbica mediante numerazione delle microcolonie eseguita al contatore elettronico di particelle