Lomofungine as an inhibitor of nucleic acid synthesis in Saccharomyces cerevisiae.
1. The antibiotic lomofungin was found to be a potent inhibitor of both DNA and RNA synthesis in Saccharomyces cerevisiae. Under selected growth conditions inhibition of DNA synthesis by the drug preceded inhibition of RNA synthesis. 2. Although in general lomofungin inhibited synthesis of ribosomal RNA and polydisperse RNA more effectively than that of low-molecular-weight RNA, under certain conditions the drug inhibited almost completely synthesis of both 4S and 5S RNA. 3. Inhibition of both RNA and DNA synthesis may be explained if RNA synthesis is required for DNA synthesis in yeast. Alternatively, lomofungin, in addition to interacting with DNA-dependent RNA polymerase, might interfere with a component(s) of the DNA-synthetic apparatus. The drug may thus prove to be of considerable value in studies of DNA synthesis in eukaryotes.
D000225:Adenine / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000096:biosynthesis*; D004261:DNA Replication / Q000187:drug effects*; D010619:Phenazines / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D013104:Spheroplasts / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects*; D014498:Uracil / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D000900:Anti-Bacterial Agents; D010619:Phenazines; D014498:Uracil; D012313:RNA; D004247:DNA; D000225:Adenine; D014529:Uridine