Purification of Pseudomonas aeruginosa endotoxin by membrane partition chromatography.
A procedure is described for obtaining large quantities of purified endotoxin of Pseudomonas aeruginosa by using Diaflo ultrafiltration. This method allowed us to isolate from the protein-lipopolysaccharide complex two low-molecular-weight substances which do not play any antigenic role. It provides a useful tool for immunological purposes.
D000818:Animals; D002473:Cell Wall; D002845:Chromatography; D002850:Chromatography, Gel; D004492:Edetic Acid; D004731:Endotoxins / Q000302:isolation & purification*; D005374:Filtration; D007106:Immune Sera; D007122:Immunoelectrophoresis; D008567:Membranes, Artificial; D008722:Methods; D011550:Pseudomonas aeruginosa; D011817:Rabbits / Q000276:immunology; D013053:Spectrophotometry; D014461:Ultracentrifugation; D014466:Ultraviolet Rays
D004731:Endotoxins; D007106:Immune Sera; D008567:Membranes, Artificial; D004492:Edetic Acid