title: Genetic regulation of cell division initiation in Bacillus subtilis.
abstract: The growth and division properties of a temperature-sensitive mutant of Bacillus subtilis defective in the initiation of cell division have been studied. Log-phase cells transferred from 30 to 45 C continue to increase in length but fail to initiate new divisions. Deoxyribonucleic acid synthesis continues at 45 C, and genomes are segregated along the filament length. When filaments are returned to 30 C, division initiation resumes, and the long multinucleate clones are partitioned into normal-size cells. Occasionally, multiple cross walls initiate in close proximity, resulting in tiny cells, some of which are anucleate. Division resumption is sensitive to protein synthesis inhibitors, suggesting there is a new protein required for the initiation of division in filaments.
mesh_terms: D001345:Autoradiography; D001412:Bacillus subtilis / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis; D002247:Carbon Isotopes; D002455:Cell Division; D002473:Cell Wall; D002701:Chloramphenicol / Q000494:pharmacology; D004269:DNA, Bacterial / Q000096:biosynthesis; D005827:Genetics, Microbial; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D009154:Mutation; D011691:Puromycin / Q000494:pharmacology; D013696:Temperature; D013941:Thymine / Q000378:metabolism; D014316:Tritium
chemical_list: D001426:Bacterial Proteins; D002247:Carbon Isotopes; D004269:DNA, Bacterial; D014316:Tritium; D011691:Puromycin; D002701:Chloramphenicol; D013941:Thymine