Analysis of free amino acid pools in fungal mycelia.
Free amino acid pools derived from three different types of fungal mycelia have been analyzed by the method of Heathcote and Haworth by using thinlayer chromatography. The preliminary extraction was carried out with boiling water and interfering proteins; peptides and salts were first removed by means of an ion-retardation resin. As far as determined, the results obtained represent the first quantitative analysis of fungal amino acid pools.
D000596:Amino Acids / Q000032:analysis* / Q000302:isolation & purification; D001230:Aspergillus / Q000032:analysis; D001231:Aspergillus flavus / Q000032:analysis / Q000254:growth & development; D002855:Chromatography, Thin Layer; D003470:Culture Media; D003720:Densitometry; D005658:Fungi / Q000032:analysis*; D006358:Hot Temperature; D007475:Ion Exchange Resins; D008722:Methods; D003904:Mitosporic Fungi / Q000032:analysis / Q000254:growth & development; D014867:Water
D000596:Amino Acids; D003470:Culture Media; D007475:Ion Exchange Resins; D014867:Water