Evidence for vectorial phosphorylation of D-fructose by intact cells of Aerobacter aerogenes.
Extracellular d-fructose was metabolized preferentially over intracellular d-fructose in growing cells of a d-fructokinaseless mutant of Aerobacter aerogenes. This finding is interpreted to indicate that phosphorylation of d-fructose by the membrane-bound phosphoenolpyruvate: d-fructose 1-phosphotransferase system in intact cells is coupled to unidirectional transport.
D001693:Biological Transport, Active; D002247:Carbon Isotopes; D002462:Cell Membrane / Q000201:enzymology; D002854:Chromatography, Paper; D004754:Enterobacter / Q000201:enzymology / Q000378:metabolism*; D005632:Fructose / Q000378:metabolism*; D005636:Fructosephosphates / Q000096:biosynthesis; D008851:Micropore Filters; D009154:Mutation; D010085:Oxidative Phosphorylation; D010728:Phosphoenolpyruvate / Q000378:metabolism; D010770:Phosphotransferases / Q000096:biosynthesis / Q000378:metabolism; D013237:Stereoisomerism; D013395:Sucrose / Q000378:metabolism
D002247:Carbon Isotopes; D005636:Fructosephosphates; D005632:Fructose; D013395:Sucrose; D010728:Phosphoenolpyruvate; D010770:Phosphotransferases