Human cartilage lysozyme.
The lysozyme content of human cartilage was measured by incubation of lyophilized, powdered cartilage in a variety of buffers and salt solutions, and the factors controlling the binding of lysozyme within cartilage were studied. Lysozyme was extracted from hyaline cartilage by buffers of pH greater than 9.0 by solutions 1 M in monovalent cations, and by solutions 0.12-0.40 M in divalent cations. The ability of cations to extract lysozyme from cartilage agreed with their known affinities for binding to chondroitin sulfate. The total extractable lysozyme content of five samples of human costal cartilage ranged from 1.45 to 3.36 mug lysozyme per mg of cartilage; for five samples of hyaline cartilage from peripheral joints the range was 0.80-3.03 mug lysozyme per mg of cartilage. Cartilage incubated in excess exogenous lysozyme could bind 0.053 equivalents of lysozyme per equivalent of chondroitin sulfate. Fibrocartilage and synovium from knee joints yielded no detectable lysozyme, despite the fact that synovium, a tissue rich in lysosomes, contained measurable quantities of beta-glucuronidase. Lysozyme extraction from cartilage was not augmented by incubation with streptolysin S. When incubation was carried out with mild extraction techniques, lysozyme extraction from cartilage tended to parallel uronic acid release, both as a function of time and from one specimen to another. The active material as lysozyme. Lysozyme occurs in human hyaline cartilage as a counterion to polyanionic glycosaminoglycans. Carextracted from cartilage met five criteria for identification tilage lysozyme appears to be extracellular and nonlysosomal. Degradation of cartilage may contribute to the increased serum and synovial fluid lysozyme levels often present in patients with rheumatoid arthritis.
D000328:Adult; D000368:Aged; D001665:Binding Sites; D002021:Buffers; D002356:Cartilage / Q000032:analysis / Q000201:enzymology*; D002358:Cartilage, Articular / Q000032:analysis / Q000201:enzymology; D002807:Chondroitin / Q000378:metabolism; D003470:Culture Media; D004573:Electrolytes / Q000494:pharmacology; D005260:Female; D005612:Freeze Drying; D005966:Glucuronidase / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008722:Methods; D008837:Micrococcus; D008875:Middle Aged; D009113:Muramidase / Q000032:analysis* / Q000378:metabolism; D013583:Synovial Membrane / Q000032:analysis / Q000201:enzymology; D013997:Time Factors; D014574:Uronic Acids / Q000032:analysis
D002021:Buffers; D003470:Culture Media; D004573:Electrolytes; D014574:Uronic Acids; D002807:Chondroitin; D009113:Muramidase; D005966:Glucuronidase