Action of dimeric hybrids of native and selectively alkylated ribonuclease A on double-stranded ribonucleic acid.
The hypothesis that both active sites of dimeric ribonuclease A have to be simultaneously available in order to make the enzyme able to degrade double-stranded RNA is tested with purified hybrid dimers of ribonuclease A and its carboxymethylated derivative. The results obtained support the hypothesis.
D002499:Centrifugation, Density Gradient; D002845:Chromatography; D002850:Chromatography, Gel; D004926:Escherichia coli; D008745:Methylation; D012313:RNA; D012260:Ribonucleases; D012709:Serum Albumin; D014316:Tritium
D012709:Serum Albumin; D014316:Tritium; D012313:RNA; D012260:Ribonucleases