Recovery of dengue viruses from tissues of experimentally infected rhesus monkeys.
A tissue explant culture technique for the recovery of dengue virus from experimentally infected monkey tissue is described and compared with tissue culture assay of tissue triturates and co-cultivation of trypsinized cells in cell cultures. The most efficient technique was one in which minced tissue was explanted in co-culture with dengue virus-susceptible LLC-MK2 monkey kidney cells. This technique shows promise of being useful for detection of virus in autopsy material from fatal dengue hemorrhagic fever cases.
D000818:Animals; D002460:Cell Line; D046508:Culture Techniques; D003715:Dengue / Q000382:microbiology*; D003716:Dengue Virus / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D005260:Female; D000882:Haplorhini; D007668:Kidney; D008251:Macaca; D008722:Methods; D009928:Organ Specificity; D014357:Trypsin; D010948:Viral Plaque Assay; D014776:Virus Cultivation
D014357:Trypsin