The copolymeric structure of pig skin dermatan suplhate. Characterization of D-glucuronic acid-containing oligosaccharides isolated after controlled degradation of oxydermatan sulphate.
Selective periodate oxidation of unsubstituted l-iduronic acid residues in copolymeric dermatan sulphate chains was followed by reduction-hydrolysis or alkaline elimination. By this procedure the glucuronic acid-containing periods were isolated in oligosaccharide form; general formula: [Formula: see text] Further degradation of these oligosaccharides with chondroitinase-AC yielded three types of products: (a) sulphated trisaccharide containing an unsaturated uronosyl moiety in the non-reducing terminal and a C(4) fragment in the reducing terminal, DeltaUA-GalNAc-(-SO(4))-R; (b) monosulphated, unsaturated disaccharide, DeltaUA-GalNAc-SO(4) when n is greater than or equal to 2; and (c) N-acetylgalactosamine with or without sulphate. Oligosaccharides containing a single glucuronic acid residue (n=1) comprised more than half of the glucuronic acid-containing oligosaccharides. The terminal N-acetylgalactosamine moiety of the shortest oligosaccharide was largely 4-sulphated, whereas higher oligosaccharides primarily contained 6-sulphated or unsulphated hexosamine moieties in the same position. Moreover, IdUA-SO(4)-containing oligosaccharides were encountered. These oligosaccharides were resistant to the action of chondroitinase-ABC.
D000818:Animals; D001173:Arthrobacter / Q000201:enzymology; D002807:Chondroitin / Q000031:analogs & derivatives*; D002852:Chromatography, Ion Exchange; D003871:Dermatan Sulfate / Q000032:analysis*; D005688:Galactosamine / Q000032:analysis; D005965:Glucuronates / Q000032:analysis*; D006595:Hexosamines / Q000032:analysis; D006603:Hexuronic Acids / Q000032:analysis; D006868:Hydrolysis; D008190:Lyases; D009844:Oligosaccharides / Q000302:isolation & purification*; D010084:Oxidation-Reduction; D011514:Proteus vulgaris / Q000201:enzymology; D013429:Sulfatases; D013552:Swine
D005965:Glucuronates; D006595:Hexosamines; D006603:Hexuronic Acids; D009844:Oligosaccharides; D003871:Dermatan Sulfate; D005688:Galactosamine; D002807:Chondroitin; D013429:Sulfatases; D008190:Lyases