title: Development of bactericidal capacity and phagocytosis-associated metabolism of fetal pig leukocytes.
abstract: Evidence that the bactericidal ability and the stimulated oxidative metabolism of leukocytes appear in parallel during fetal development of the Minnesota Miniature pig has been obtained by application of the techniques applied to studies of human cells. It was demonstrated that leukocytes from 87- to 90-day fetuses were fully capable of ingesting Staphylococcus aureus but greatly diminished in bactericidal capacity as compared to leukocytes of older fetuses and adults. Although resting levels of oxygen consumption and hexose monophosphate pathway activity of leukocytes from the younger fetuses compared well with those of leukocytes from older animals, the phagocytosis-stimulated increments of metabolism were much less at 87 to 90 days of gestation than at later developmental stages. Both bactericidal capacity and increased metabolism of leukocytes reach adult levels by 100 days of gestation (normal gestation period of 115 to 120 days). Acrylamide gels stained for reduced nicotinamide adenine dinucleotide (NADH) and NADH phosphate (NADPH) diaphorase activity after disc electrophoresis of leukocyte extracts revealed normal mobility and intensity of NADH diaphorase bands. Three NADPH diaphorase bands were present in adult leukocyte extracts. Only the fast-migrating NADPH diaphorase band of 87- to 90-day cells stained with decreased intensity. This "deficiency" was no longer present at the later fetal period. The fast-migrating NADPH diaphorase band may represent an electron transfer protein which functions in cyanide-insensitive respiration of the leukocytes of the pig.
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000367:Age Factors; D000818:Animals; D001770:Blood Bactericidal Activity; D002247:Carbon Isotopes; D004591:Electrophoresis, Polyacrylamide Gel; D005314:Embryonic and Fetal Development; D005947:Glucose / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D006019:Glycolysis; D006600:Hexosephosphates / Q000378:metabolism; D007773:Lactates / Q000096:biosynthesis; D007962:Leukocytes / Q000201:enzymology / Q000276:immunology / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism; D009113:Muramidase / Q000032:analysis; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D010544:Peroxidases / Q000032:analysis; D010587:Phagocytosis; D013210:Staphylococcus; D013552:Swine / Q000276:immunology
chemical_list: D002247:Carbon Isotopes; D006600:Hexosephosphates; D007773:Lactates; D005954:Glucosephosphate Dehydrogenase; D010544:Peroxidases; D000135:Acid Phosphatase; D009113:Muramidase; D005947:Glucose