Increased stability and reproducibility of Escherichia coli spheroplasts in the transfection assay of phage lambda DNA with polyethylene glycol instead of sucrose.
D001431:Bacteriological Techniques; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D003090:Coliphages / Q000302:isolation & purification; D003470:Culture Media; D004269:DNA, Bacterial; D004279:DNA, Viral / Q000302:isolation & purification / Q000494:pharmacology*; D004492:Edetic Acid; D004926:Escherichia coli; D006018:Glycols; D007740:L Forms; D009113:Muramidase; D011095:Polyethylenes; D011479:Protamines; D013104:Spheroplasts / Q000254:growth & development; D013395:Sucrose; D014170:Transformation, Genetic
D002712:Chlorides; D003470:Culture Media; D004269:DNA, Bacterial; D004279:DNA, Viral; D006018:Glycols; D011095:Polyethylenes; D011479:Protamines; D002586:Cesium; D013395:Sucrose; D004492:Edetic Acid; D009113:Muramidase