3-phosphoglycerate kinase from Hydrogenomonas facilis.
Phosphoglycerate kinase levels in Hydrogenomonas facilis were reasonably constant whether cells were utilizing or synthesizing hexose during growth. Specific enzyme activities (micromoles of 3-phosphoglycerate disappearing per minute per milligram of protein) at 30 C were 0.234, 0.391, 0.300, and 0.229 in the "soluble" fraction derived from cells grown on fructose, lactate, succinate, and glutamate, respectively. The enzyme was purified 300-fold from succinate-grown cells. The final preparation, which was not homogenous but was free from glyceraldehyde-3-phosphate dehydrogenase and adenylate kinase, had a specific activity at 30 C of 90 mumoles of 3-phosphoglycerate per min per mg of protein. K(m) values for adenosine triphosphate (ATP), 3-phosphoglycerate, and Mg(++) were 0.16, 0.83, and 0.4 mm, respectively, at pH 7.4 and 30 C. Adenosine monophosphate (AMP) inhibited 23% at a ratio of AMP to ATP of 2.4, and the possible physiological implications of this inhibition are discussed. No evidence was found for an enzyme which catalyzes ATP-dependent conversion of 3-phosphoglycerate to 1,3-diphosphoglycerate, AMP, and phosphate.
D000249:Adenosine Monophosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism; D000645:Ammonium Sulfate; D002384:Catalysis; D002474:Cell-Free System; D011232:Chemical Precipitation; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003470:Culture Media; D005632:Fructose / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism; D006358:Hot Temperature; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D008274:Magnesium; D008345:Manganese; D010735:Phosphoglycerate Kinase / Q000032:analysis* / Q000302:isolation & purification; D011549:Pseudomonas / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D013053:Spectrophotometry; D013386:Succinates / Q000378:metabolism
D003470:Culture Media; D005971:Glutamates; D005994:Glycerophosphates; D007773:Lactates; D013386:Succinates; D005632:Fructose; D000249:Adenosine Monophosphate; D008345:Manganese; D000255:Adenosine Triphosphate; D010735:Phosphoglycerate Kinase; D008274:Magnesium; D000645:Ammonium Sulfate