Bacteriolytic enzymes produced by Myxococcus xanthus.
The bacteriolytic activities in the culture fluid of Myxococcus xanthus were purified and separated into six active fractions by the use of Bio-Gel CM-2 and Bio-Gel P-60. These fractions were identified as: (i) an amidase, (ii) a glucosaminidase, (iii) a glucosaminidase and an amidase, (iv) a protease with probable amidase activity, (v) another protease with probable amidase activity, and (vi) a peptidase active on both d-alanyl-diaminopimelate and d-alanyl-lysine peptide bonds. On one occasion, another amidase was eluted from Bio-Gel CM. Preliminary studies on some characteristics of the enzymes and their production during growth are reported.
D000409:Alanine / Q000378:metabolism; D000581:Amidohydrolases / Q000302:isolation & purification*; D000596:Amino Acids; D001412:Bacillus subtilis / Q000166:cytology / Q000378:metabolism; D001419:Bacteria / Q000201:enzymology* / Q000254:growth & development; D001433:Bacteriolysis; D002247:Carbon Isotopes; D002473:Cell Wall / Q000187:drug effects; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D010450:Endopeptidases / Q000302:isolation & purification*; D006026:Glycoside Hydrolases / Q000302:isolation & purification*; D006596:Hexosaminidases / Q000302:isolation & purification; D007553:Isotope Labeling; D008239:Lysine; D008837:Micrococcus / Q000166:cytology / Q000187:drug effects; D010447:Peptide Hydrolases / Q000302:isolation & purification*; D010457:Peptidoglycan; D010867:Pimelic Acids; D013210:Staphylococcus / Q000166:cytology
D000596:Amino Acids; D002247:Carbon Isotopes; D010457:Peptidoglycan; D010867:Pimelic Acids; D006026:Glycoside Hydrolases; D006596:Hexosaminidases; D010450:Endopeptidases; D010447:Peptide Hydrolases; D000581:Amidohydrolases; D008239:Lysine; D000409:Alanine