A modified procedure for fractionating histones.
A method is described, which is capable of fractionating histones obtained from any animal source into five major groups. Although the method is based on procedures initially developed by E. W. Johns (1964), involving differential solubility in solutions of acids and of ethanol, it gives a cleaner separation and possesses the considerable advantage that the starting material is whole histone rather than a nucleoprotein preparation.
D000818:Animals; D002417:Cattle; D002845:Chromatography; D004331:Drosophila melanogaster; D004586:Electrophoresis; D005399:Fishes; D006657:Histones / Q000032:analysis* / Q000302:isolation & purification; D006801:Humans; D008099:Liver / Q000032:analysis; D008722:Methods; D009698:Nucleoproteins / Q000032:analysis; D010084:Oxidation-Reduction; D010920:Placenta / Q000032:analysis; D012995:Solubility; D013950:Thymus Gland / Q000032:analysis
D006657:Histones; D009698:Nucleoproteins