Identification of polysomal RNA in BHK cells infected by sindbis virus.
Polysomes were prepared from Sindbis virus-infected BHK cells. The major species of RNA in these polysomes was identified as 26S RNA (interjacent RNA) by (i) disrupting the polysomes with EDTA; (ii) treating the infected cells with puromycin; and (iii) isolating polysomes from cells infected with a temperature-sensitive mutant that does not form nucleocapsids. Small amounts of 42S RNA and 33S RNA were also found in polysomes.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002247:Carbon Isotopes; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D004492:Edetic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D007668:Kidney; D009154:Mutation; D011132:Polyribosomes / Q000032:analysis*; D011691:Puromycin / Q000494:pharmacology; D012335:RNA, Ribosomal / Q000032:analysis* / Q000096:biosynthesis / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis* / Q000096:biosynthesis / Q000302:isolation & purification; D012845:Sindbis Virus / Q000032:analysis / Q000254:growth & development* / Q000378:metabolism; D013696:Temperature; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D000596:Amino Acids; D002247:Carbon Isotopes; D012335:RNA, Ribosomal; D012367:RNA, Viral; D014316:Tritium; D011691:Puromycin; D004492:Edetic Acid; D014529:Uridine