Selective replication of bacteriophage phi29 deoxyribonucleic acid in 6-(p-hydroxyphenylazo)-uracil-treated Bacillus subtilis.
Phage phi29 deoxyribonucleic acid (DNA) replicated under conditions where semiconservative DNA production in Bacillus subtilis host cells was blocked with 6-(p-hydroxyphenylazo)-uracil (HPUra). The time of initiation of phi29 DNA replication was not affected by HPUra, and normal quantities of viable phage were produced in the presence of the inhibitor. Studies with conditional lethal mutants of phage phi29 demonstrated the usefulness of HPUra for detection of viral-specific DNA production.
D001391:Azo Compounds / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects* / Q000378:metabolism; D001435:Bacteriophages / Q000254:growth & development / Q000378:metabolism*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis; D004279:DNA, Viral / Q000096:biosynthesis*; D005804:Genes, Lethal; D008937:Mitomycins / Q000494:pharmacology; D009154:Mutation; D009693:Nucleic Acid Hybridization; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium; D014498:Uracil; D014779:Virus Replication
D001391:Azo Compounds; D004269:DNA, Bacterial; D004279:DNA, Viral; D008937:Mitomycins; D014316:Tritium; D014498:Uracil; D013936:Thymidine