Studies on the particulate lactose synthetase of mouse mammary gland and the role of  -lactalbumin in the initiation of lactose synthesis.
1. Some of the kinetic properties of the particulate lactose synthetase of mouse mammary gland were investigated and shown to resemble those that have been reported for the soluble enzyme. Typical values for intact preparations were 2.3mum for the apparent K(m) for alpha-lactalbumin at 40mm-glucose and 1.7mm for the apparent K(m) for glucose at the endogenous concentration of alpha-lactalbumin. 2. Digitonin treatment increased total assayable activity approximately twofold but almost eliminated the endogenous activity found in the absence of alpha-lactalbumin, these findings being consistent with the location of endogenous activity within Golgi vesicles. 3. From the properties of the particulate fraction from lactating mice it was deduced that the effective endogenous alpha-lactalbumin concentrations were in the range 1-10mum. 4. The concentration of alpha-lactalbumin was not significantly different in particles isolated at various stages of pregnancy and early lactation. 5. The implications of these results for the control of lactose synthetase in vivo are discussed.
D000818:Animals; D005260:Female; D005947:Glucose; D006056:Golgi Apparatus / Q000201:enzymology; D006602:Hexosyltransferases; D007700:Kinetics; D007743:Labor, Obstetric; D007768:Lactalbumin; D007774:Lactation; D007785:Lactose / Q000096:biosynthesis*; D007788:Lactose Synthase; D008321:Mammary Glands, Animal / Q000201:enzymology*; D051379:Mice; D009097:Multienzyme Complexes; D011247:Pregnancy; D012503:Saponins; D013385:Succinate Dehydrogenase / Q000032:analysis
D009097:Multienzyme Complexes; D012503:Saponins; D007768:Lactalbumin; D013385:Succinate Dehydrogenase; D006602:Hexosyltransferases; D007788:Lactose Synthase; D005947:Glucose; D007785:Lactose