Purification and properties of the adenosine diphosphoglucose: glycogen transglucosylase of Pasteurella pseudotuberculosis.
The enzyme which catalyzes the transfer of glucosyl residues from adenosine diphospho(ADP)-glucose to glycogen has been partially purified from extracts of Pasteurella pseudotuberculosis. In contrast to other glycogen synthetases of this type, guanosine diphospho-glucose had about 5% of the activity of ADP-glucose as a glucosyl donor. Some other properties of the enzyme are described and compared to other bacterial glycogen synthetases.
D000244:Adenosine Diphosphate; D000645:Ammonium Sulfate; D002247:Carbon Isotopes; D002474:Cell-Free System; D002614:Chelating Agents / Q000494:pharmacology; D011232:Chemical Precipitation; D002845:Chromatography; D002850:Chromatography, Gel; D004579:Electron Transport; D005947:Glucose / Q000378:metabolism; D005964:Glucosyltransferases; D006003:Glycogen / Q000096:biosynthesis; D006006:Glycogen Synthase / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000494:pharmacology; D009711:Nucleotides / Q000378:metabolism / Q000494:pharmacology; D010325:Pasteurella / Q000201:enzymology*; D011134:Polysaccharides / Q000378:metabolism; D013439:Sulfhydryl Reagents / Q000494:pharmacology
D002247:Carbon Isotopes; D002614:Chelating Agents; D008670:Metals; D009711:Nucleotides; D011134:Polysaccharides; D013439:Sulfhydryl Reagents; D000244:Adenosine Diphosphate; D006003:Glycogen; D005964:Glucosyltransferases; D006006:Glycogen Synthase; D005947:Glucose; D000645:Ammonium Sulfate