Effects of some disinfectants on African swine fever virus.
Ten commercially available disinfectants were tested at high pH in 2% sodium hydroxide and low pH in 2% acetic acid as inactivants for African swine fever (ASF) in a protein-rich blood-spleen homogenate. As assayed in leukocyte cultures, sodium hydroxide and acetic acid, sodium meta silicate and Roccal did not inactivate ASF virus in 1 hr at 22 to 25 C. Some viricidal activity as assayed in leukocyte cultures was found with Weladol, Triton X-100 Amphyl, pHisoHex, sodium dodecyl sulfate, LpH, Environ, Environ D, and One-Stroke Environ. Of these, the last four appeared to be most promising. When assayed in pigs, only One-Stroke Environ (1/E) was viricidal. Concentrations of 1.0, 0.75, and 0.5 were effective, but, at 0.25%, virus was not inactivated. The minimal time to inactivate ASF virus by 1% 1/E is 60 min. A room contaminated with ASF virus was made safe for pigs after 1 hr by spraying with 1% 1/E. The most active component of 1/E is o-phenylphenol. Although another component of 1/E, i.e., o-benzyl-p-chlorophenol, also has some activity, the mixture of the active components of 1/E is most effective against ASF virus. One of the soluble antigens associated with ASF virus is destroyed by 1/E.
D000085:Acetates; D000357:African Swine Fever / Q000382:microbiology*; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D046508:Culture Techniques; D004267:DNA Viruses / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity; D004202:Disinfectants / Q000494:pharmacology*; D000882:Haplorhini; D006382:Hemadsorption; D006863:Hydrogen-Ion Concentration; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007668:Kidney; D007962:Leukocytes; D010636:Phenols / Q000494:pharmacology; D012972:Sodium Hydroxide; D012997:Solvents; D013552:Swine; D013997:Time Factors
D000085:Acetates; D000956:Antigens, Viral; D000998:Antiviral Agents; D004202:Disinfectants; D010636:Phenols; D012997:Solvents; D012972:Sodium Hydroxide