title: Immunochemistry and end-group analyses of group A streptococcal M proteins.
abstract: Type-specific M proteins were examined to determine whether their immunological specificities were also reflected by major chemical differences. Sixteen different type-specific protein preparations were employed, including two from non-M-typable strains. These M proteins, acid-extracted from whole cells, were purified by ammonium sulfate fractionation and column chromatography and were compared by immunodiffusion, electrophoretic, amino acid, and N-terminal amino acid analyses. Although the data did not reflect major chemical distinctiveness in the types examined, some interesting results evolved. Four important factors were observed to be shared by all M protein types examined: (i) glutamic acid was the most prevalent amino acid, (ii) amino acid molar ratios were similar, (iii) each had l-alanine as a single N-terminus, and (iv) purified peaks from the column chromatograms still showed heterogeneity while giving type-specific reactivity for multiple bands. Thus, whereas chemical typing is apparently unfeasible, the data indicate that these may be unique proteins, reflected especially in the finding of the same N-terminus amino acid in all strains investigated.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000645:Ammonium Sulfate; D001426:Bacterial Proteins / Q000032:analysis* / Q000302:isolation & purification; D002855:Chromatography, Thin Layer; D004140:Dinitrophenols; D004589:Electrophoresis, Disc; D007120:Immunochemistry; D005779:Immunodiffusion; D010443:Peptide Chain Termination, Translational; D013056:Spectrophotometry, Ultraviolet; D013291:Streptococcus / Q000032:analysis*
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D004140:Dinitrophenols; D000645:Ammonium Sulfate