title: Biosynthesis of glycogen and starch in Cryptococcus laurentii.
abstract: Cells of Cryptococcus laurentii, when grown in liquid culture on 2% glucose close to neutral pH, showed glycogen granules throughout the cytoplasm. Glycogen levels of C. laurentii cells reached maximal levels just before onset of stationary phase. Concomitantly, a sharp rise in total and specific activity of glycogen synthetase was observed. Conversely, glycogen phosphorylase reached its highest specific activity approximately 3 hr after the glycogen peaked and remained high until most of the endogenous glycogen was utilized. Uridine diphosphoglucose pyrophosphorylase activity was always an order of magnitude higher than glycogen synthetase during log phase, but fell off rapidly after the cells reached stationary growth. Kinetic properties of the glycogen synthetase showed that the enzyme is always activated by glucose-6-phosphate, although the degree of activation by glucose-6-phosphate was found to be somewhat variable. The accelerated uptake of glucose commencing with the onset of stationary phase is explained by the rapid formation of extracellular acidic polysaccharide, which continues as long as there is glucose in the medium. In cells grown at pH 3.4, where no detectable extracellular acidic polysaccharide was formed, glucose uptake drastically declined when the cells reached stationary phase. These cells also contained glycogen-like granules in the cytoplasm. The evidence presented indicates that these granules are in fact glycogen, and that its structure does not resemble that of the starch excreted by cells grown at acidic pH.
mesh_terms: D002247:Carbon Isotopes; D002474:Cell-Free System; D002854:Chromatography, Paper; D003454:Cryptococcus / Q000166:cytology / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media; D003594:Cytoplasmic Granules; D005947:Glucose / Q000378:metabolism; D005958:Glucosephosphates / Q000494:pharmacology; D006003:Glycogen / Q000096:biosynthesis* / Q000378:metabolism; D006006:Glycogen Synthase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D006005:Phosphorylases / Q000378:metabolism; D011117:Polyribonucleotide Nucleotidyltransferase / Q000378:metabolism; D011134:Polysaccharides / Q000096:biosynthesis; D013053:Spectrophotometry; D013213:Starch / Q000096:biosynthesis*
chemical_list: D002247:Carbon Isotopes; D003470:Culture Media; D005958:Glucosephosphates; D011134:Polysaccharides; D013213:Starch; D006003:Glycogen; D006005:Phosphorylases; D006006:Glycogen Synthase; D011117:Polyribonucleotide Nucleotidyltransferase; D005947:Glucose