Indirect fluorescent-antibody and quantitative agar-gel immunodiffusion tests for the serological diagnosis of paracoccidioidomycosis.
The value of various serological tests in the diagnosis of paracoccidioidomycosis was studied. Quantitative agar-gel immunodiffusion and indirect immunofluorescent tests were performed, and the results were compared with those of complement fixation and qualitative agar-gel procedures. The quantitative immunodiffusion procedure was found to serve as the simplest and safest quantitative test that could be performed for evaluation purposes, whereas the indirect fluorescent-antibody test gave nonspecific reactions and, as such, proved unsuitable.
D000362:Agar; D000818:Animals; D000941:Antigens; D001759:Blastomycosis / Q000175:diagnosis* / Q000276:immunology; D003047:Coccidioidomycosis / Q000276:immunology; D003168:Complement Fixation Tests; D003429:Cross Reactions; D003937:Diagnosis, Differential; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D005658:Fungi / Q000276:immunology*; D006660:Histoplasmosis / Q000276:immunology; D006801:Humans; D007106:Immune Sera; D005779:Immunodiffusion; D008722:Methods; D010228:Paracoccidioides / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D012698:Serologic Tests; D014376:Tuberculosis / Q000276:immunology
D000941:Antigens; D007106:Immune Sera; D000362:Agar