Phycobilisomes in Anacystis nidulans.
Phycobilisomes were demonstrated in Anacystis nidulans by chemical and morphological studies on cells grown in red light. These cells showed a marked reduction in the chlorophyll-phycocyanin ratio owing to a decreased chlorophyll content. Granular structures of approximately 35 nm were observed throughout red light-grown cells, but were most distinct in the peripheral region. The presence of phycobilisomes in cells grown in red light as well as in cells grown in white light is supported by experiments in which glutaraldehyde was used to stabilize the attachment between the phycobiliprotein and the thylakoids, allowing the isolation of both in the same fraction by sucrose density gradient centrifugation.
D000447:Aldehydes; D002499:Centrifugation, Density Gradient; D002734:Chlorophyll / Q000032:analysis; D000458:Cyanobacteria / Q000032:analysis / Q000166:cytology* / Q000254:growth & development; D008027:Light; D008854:Microscopy, Electron; D009940:Organoids; D045524:Phycobilisomes; D010860:Pigments, Biological / Q000032:analysis; D010940:Plant Proteins / Q000032:analysis; D013053:Spectrophotometry
D000447:Aldehydes; D045524:Phycobilisomes; D010860:Pigments, Biological; D010940:Plant Proteins; D002734:Chlorophyll