title: Ribosomal ribonucleic acid and ribosomal precursor ribonucleic acid in Anacystis nidulans.
abstract: The RNA of the blue-green alga Anacystis nidulans contains three ribosomal RNA species with molecular weights of 0.56x10(6), 0.9x10(6), and 1.1x10(6) if the RNA is extracted in the absence of Mg(2+). The 0.9x10(6)mol.wt. rRNA is extremely slowly labelled in (32)P-incorporation experiments. This rRNA may be a cleavage product of the 1.1x10(6)mol.wt. rRNA from the ribosomes of cells in certain physiological states (e.g. light-deficiency during growth). The cleavage of the 1.1x10(6)mol.wt. rRNA during the extraction procedure can be prevented by the addition of 10mm-MgCl(2). (32)P-pulse-labelling studies demonstrate the rapid synthesis of two ribosomal precursor RNA species. One precursor RNA migrating slightly slower than the 1.1x10(6)mol.wt. rRNA appears much less stable than the other precursor RNA, which shows the electrophoretic behaviour of the 0.7x10(6)mol.wt. rRNA. Our observations support the close relationship between bacteria and blue-green algae also with respect to rRNA maturation. The conversion of the ribosomal precursor RNA species into 0.56x10(6)- and 1.1x10(6)-mol.wt. rRNA species requires Mg(2+) in the incubation medium.
mesh_terms: D000458:Cyanobacteria / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D008274:Magnesium; D008970:Molecular Weight; D010759:Phosphorus Isotopes; D012313:RNA / Q000032:analysis*; D012335:RNA, Ribosomal / Q000032:analysis*
chemical_list: D010759:Phosphorus Isotopes; D012335:RNA, Ribosomal; D012313:RNA; D008274:Magnesium