Synthesis of ceramides using N-hydroxysuccinimide esters.
Fatty acyl esters of N-hydroxysuccinimide have been used to directly N-acylate sphingenine or sphinganine, forming the corresponding ceramides. The reaction proceeds in excellent yield (84-96%) from small amounts of starting material (10-20 mg). The product ceramides are pure after one recrystallization.
D002247:Carbon Isotopes; D002518:Ceramides / Q000138:chemical synthesis*; D055598:Chemical Phenomena; D002621:Chemistry; D002855:Chromatography, Thin Layer; D004952:Esters; D005227:Fatty Acids; D006900:Hydroxylation; D008722:Methods; D010169:Palmitic Acids; D011760:Pyrrolidinones; D013055:Spectrophotometry, Infrared; D013229:Stearic Acids; D013388:Succinimides
D002247:Carbon Isotopes; D002518:Ceramides; D004952:Esters; D005227:Fatty Acids; D010169:Palmitic Acids; D011760:Pyrrolidinones; D013229:Stearic Acids; D013388:Succinimides