Fate of transforming deoxyribonucleic acid after uptake by competent Bacillus subtilis: size and distribution of the integrated donor segments.
A series of sheared donor-recipient complex samples were prepared from a competent Bacillus subtilis culture which had been transformed by using (3)H, (2)H-labeled deoxyribonucleic acid. Measurement of the molecular weights and buoyant densities of these samples led to the following conclusions. (i) The average size of the single-stranded donor segment which is integrated is 2.8 x 10(6). (ii) These donor segments are not randomly distributed in the recipient genome, but are clustered in groups, each group containing several discrete donor segments.
D001412:Bacillus subtilis / Q000378:metabolism; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D003090:Coliphages; D004269:DNA, Bacterial / Q000032:analysis / Q000378:metabolism; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D005827:Genetics, Microbial; D008970:Molecular Weight; D013941:Thymine / Q000378:metabolism; D014170:Transformation, Genetic; D014316:Tritium
D002247:Carbon Isotopes; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D014316:Tritium; D013941:Thymine