Base exchange reactions of the phospholipids in rat brain particles.
A particulate fraction from rat brain catalyzes the incorporation of [(14)C]choline, [(14)C]ethanolamine, and l-[(14)C]serine into phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine, respectively. The reaction appears to be energy-independent since Mg(2+), CTP, ATP, and NaF have no stimulatory action. The incorporation is inhibited by EDTA and activated by Ca(2+). The pH optimum for the incorporation of choline is 9.5, for ethanolamine it is 9.0, and for l-serine it is 8.5. Tris, bicine, and imidazole buffers are inhibitory. The incorporations are inhibited by a variety of structurally related alcohols and are stimulated by isoserine (alpha-hydroxy,beta-aminopropionic acid).
D000438:Alcohols / Q000494:pharmacology; D000605:Amino Alcohols / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D002247:Carbon Isotopes; D002794:Choline / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D010714:Phosphatidylethanolamines / Q000096:biosynthesis*; D010740:Phospholipases / Q000494:pharmacology; D011422:Propionates / Q000494:pharmacology; D051381:Rats; D012694:Serine / Q000378:metabolism; D013329:Structure-Activity Relationship
D000438:Alcohols; D000605:Amino Alcohols; D002247:Carbon Isotopes; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D011422:Propionates; D012694:Serine; D004492:Edetic Acid; D010740:Phospholipases; D002794:Choline; D002118:Calcium