title: New deoxyribonucleic acid polymerase induced by Bacillus subtilis bacteriophage PBS2.
abstract: The deoxyribonucleic acid (DNA) of Bacillus subtilis phage PBS2 has been confirmed to contain uracil instead of thymine. PBS2 phage infection of wild-type cells or DNA polymerase-deficient cells results in an increase in the specific activity of DNA polymerase. This induction of DNA polymerase activity is prevented by actinomycin D and chloramphenicol. In contrast to the major B. subtilis DNA polymerase, which prefers deoxythymidine triphosphate (dTTP) to deoxyuridine triphosphate (dUTP), the DNA polymerase in crude extracts of PBS2-infected cells is equally active whether dTTP or dUTP is employed. This phage-induced polymerase may be responsible for the synthesis of uracil-containing DNA during PBS2 phage infection.
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology*; D001435:Bacteriophages / Q000032:analysis / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D002474:Cell-Free System; D002701:Chloramphenicol / Q000494:pharmacology; D002854:Chromatography, Paper; D003124:Colorimetry; D004254:DNA Nucleotidyltransferases / Q000096:biosynthesis / Q000378:metabolism*; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis; D003609:Dactinomycin / Q000494:pharmacology; D003853:Deoxyribonucleosides / Q000378:metabolism; D004790:Enzyme Induction; D008274:Magnesium / Q000494:pharmacology; D013941:Thymine / Q000032:analysis; D013997:Time Factors; D014498:Uracil / Q000032:analysis; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D003853:Deoxyribonucleosides; D003609:Dactinomycin; D014498:Uracil; D002701:Chloramphenicol; D004254:DNA Nucleotidyltransferases; D008274:Magnesium; D013941:Thymine