Infected surface wound: an experimental model and a method for the quantitation of bacteria in infected tissues.
Methods for the quantitation of bacteria in infected tissues must be rigidly standardized to insure uniformity of results. In this communication we report on a laboratory animal model for the study of surface wound infection and the development of a standardized method for the quantitative estimation of bacteria in infected surface wound tissue by mechanical tissue homogenization and serial dilution. Parallel comparative studies demonstrated that a moist-swab sampling procedure detected only 10% of the bacteria recoverable by a surface-wash procedure. Either tissue homogenization or surface-wash procedures recovered significantly more bacteria from contaminated surface wounds than were obtained by surface-swab sampling techniques.
D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000295:instrumentation; D002452:Cell Count; D003470:Culture Media; D004195:Disease Models, Animal; D005260:Female; D009132:Muscles; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains; D016038:Skin Transplantation; D013997:Time Factors; D014020:Tissue Extracts; D014182:Transplantation, Autologous; D014946:Wound Infection / Q000382:microbiology*
D003470:Culture Media; D014020:Tissue Extracts