Replication of colicinogenic factor E 1 DNA: evidence for a discontinuous replication mechanism.
The mechanism of Col E 1 DNA replication was investigated in a plasmolysed cell system prepared from chloramphenicoltreated E. coli JC 411 (Col E 1). After pulse-labelling with (3)H-dTTP a considerable fraction of the newly synthesized DNA was recovered as single-stranded fragments. Upon alkali denaturation the pulse label was found in DNA chains sedimenting slower than unit length Col E 1 strands with a prominent peak at 5 S. During a chase with unlabeled precursors the label is transferred nearly completely into supercoiled Col E 1 DNA. DNA ligase appears to be required for the joining of the 5 S pieces since in the absence of NAD an accumulation of short fragments is observed.
D001426:Bacterial Proteins / Q000096:biosynthesis; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D003087:Colicins / Q000096:biosynthesis*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000302:isolation & purification; D004277:DNA, Single-Stranded / Q000096:biosynthesis / Q000302:isolation & purification; D004926:Escherichia coli / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D013941:Thymine / Q000378:metabolism; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D003087:Colicins; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D013941:Thymine