Contribution of humoral and cellular factors to the resistance to experimental infection by Pseudomonas aeruginosa in mice. II. Opsonic, agglutinative, and protective capacities of immunoglobulin G anti-Pseudomonas antibodies.
The opsonic, agglutinative, and mouse protective capacities of anti-Pseudomonas antibodies in immune and normal human immunoglobulin G (IgG) preparations were investigated. Opsonic activities of the immune IgG preparations correlated well with their protective activities. Antibodies present in normal IgG showed a substantial agglutinative activity but were poorly protective. Anti-Pseudomonas antibodies of both normal and immune IgG preparations were directed against the same serotype antigen as demonstrated by absorption experiments. Immune anti-Pseudomonas IgG antibodies fixed complement very efficiently as demonstrated by opsonophagocytic and hemolytic tests. Natural anti-Pseudomonas IgG antibodies fixed complement very poorly although they promoted phagocytic killing of bacteria only in the presence of heat-labile serum factors. It was concluded that, although agglutination can be used for qualitative measurement of antibacterial antibodies, it fails to measure their functional capacities.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000918:Antibody Specificity; D001770:Blood Bactericidal Activity; D002470:Cell Survival; D003168:Complement Fixation Tests; D006382:Hemadsorption; D006386:Hemagglutination Tests; D006461:Hemolysis; D006358:Hot Temperature; D007109:Immunity; D007074:Immunoglobulin G / Q000032:analysis*; D007958:Leukocyte Count; D008264:Macrophages; D051379:Mice; D008815:Mice, Inbred Strains; D009895:Opsonin Proteins / Q000032:analysis*; D010587:Phagocytosis; D011552:Pseudomonas Infections / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000276:immunology*
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D009895:Opsonin Proteins