Hybridization of variants of chloramphenicol acetyltransferase specified by fi +  and fi -  R factors.
An fi(-) type of R factor for transferable chloramphenicol resistance carries the structural gene for a novel species of chloramphenicol acetyltransferase (EC 2.3.1.99). The enzyme associated with the fi(-) plasmid is distinct from that described for fi(+) R factors. The fi(+)- and fi(-)-related variants of chloramphenicol acetyltransferase were hybridized in vivo and in vitro. Both techniques yielded only a single symmetrical heteromeric species rather than the three (A(3)B(1), A(2)B(2), and A(1)B(3)) hybrids predicted from the tetrameric (identical monomers) structure of chloramphenicol acetyltransferase.
D000123:Acetyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D000918:Antibody Specificity; D002701:Chloramphenicol; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004352:Drug Resistance, Microbial; D004589:Electrophoresis, Disc; D004926:Escherichia coli / Q000201:enzymology*; D005111:Extrachromosomal Inheritance; D005796:Genes; D006041:Goats / Q000276:immunology; D006824:Hybridization, Genetic; D007106:Immune Sera; D005779:Immunodiffusion; D007525:Isoelectric Focusing; D011233:Precipitin Tests
D007106:Immune Sera; D002701:Chloramphenicol; D000123:Acetyltransferases