Template specificity of ribonucleic acid polymerase in asporogenous mutants of Bacillus subtilis.
Asporogenous mutants of Bacillus subtilis were examined for the change in template specificity of ribonucleic acid (RNA) polymerase characteristic of wild-type cells undergoing sporulation. Mutants blocked at stages II, III, and IV showed a changed specificity of the enzyme after the end of growth and were in this respect indistinguishable from the wild type. The RNA polymerase of eight stage-zero mutants (out of nine tested) which possess mutations that map at six distinct loci retained the template specificity of vegetative cells.
D000255:Adenosine Triphosphate / Q000378:metabolism; D001412:Bacillus subtilis / Q000201:enzymology* / Q000254:growth & development; D002474:Cell-Free System; D002874:Chromosome Mapping; D003090:Coliphages; D004279:DNA, Viral; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005827:Genetics, Microbial; D009154:Mutation; D011119:Polynucleotides; D013171:Spores, Bacterial / Q000254:growth & development; D013698:Templates, Genetic; D013997:Time Factors; D014158:Transcription, Genetic; D014316:Tritium; D014500:Uracil Nucleotides / Q000378:metabolism
D004279:DNA, Viral; D011119:Polynucleotides; D014500:Uracil Nucleotides; D014316:Tritium; D000255:Adenosine Triphosphate; D012321:DNA-Directed RNA Polymerases