title: Ligand-induced movement of lymphocyte membrane macromolecules. I. Analysis by immunofluorescence and ultrastructural radioautography.
abstract: The fate of different complexes on the membrane of thymocytes and spleen lymphocytes was studied with the use of both immunofluorescence and ultrastructural radioautography. The complexes of anti-immunoglobulin (Ig) with the surface Ig of B lymphocytes were present all around the membrane at 4 degrees C; an increase in temperature produced a rapid aggregation of the complex into a cap which was readily interiorized in vesicles. Ultrastructural details of this process were given. The movement of the complexes depended upon the amount of anti-Ig and the temperature. The complexes of anti-lymphocyte antibody with surface antigen(s) did not result in formation of a single large aggregate (or cap) unless an anti-antibody was brought into the reaction. The caps formed by this trilayered complex were not interiorized. Concanavalin A (Con A) bound to cell surface carbohydrate moieties and the complexes of Con A readily formed a cap and were interiorized. Finally, antibodies to H-2 determinants did not form in most instances a single cap aggregate even when anti-antibodies were used. With time the H-2 complexes tended to form several large aggregates with some endocytosis.
mesh_terms: D000818:Animals; D000961:Antilymphocyte Serum; D001345:Autoradiography; D002462:Cell Membrane / Q000276:immunology; D002848:Chromatography, DEAE-Cellulose; D003208:Concanavalin A; D005455:Fluorescent Antibody Technique; D006648:Histocompatibility; D007106:Immune Sera; D005779:Immunodiffusion; D007074:Immunoglobulin G; D007456:Iodine Isotopes; D007519:Isoantigens; D008214:Lymphocytes / Q000276:immunology*; D046911:Macromolecular Substances; D051379:Mice; D008854:Microscopy, Electron; D010206:Papain / Q000494:pharmacology; D011817:Rabbits / Q000276:immunology; D051381:Rats; D012756:Sheep / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology
chemical_list: D000961:Antilymphocyte Serum; D007106:Immune Sera; D007074:Immunoglobulin G; D007456:Iodine Isotopes; D007519:Isoantigens; D046911:Macromolecular Substances; D003208:Concanavalin A; D010206:Papain