The adverse effect of bromelin in anti-D quantitation using the AutoAnalyzer.
With eight of 32 sera significantly higher estimations of anti-D concentration were obtained when test cells premodified with bromelin were used instead of bromelin as a reagent added to the reaction manifold. With one serum the difference in estimation, using the two techniques, revealed a ten-fold discrepancy. The consequences of these findings are discussed in relation to the use of the AutoAnalyzer for the quantitation of anti-D.
D001322:Autoanalysis / Q000592:standards*; D001963:Bromelains; D006386:Hemagglutination Tests / Q000592:standards*; D006801:Humans; D007106:Immune Sera; D007202:Indicators and Reagents; D007518:Isoantibodies / Q000032:analysis*; D008722:Methods; D012204:Rh-Hr Blood-Group System
D007106:Immune Sera; D007202:Indicators and Reagents; D007518:Isoantibodies; D012204:Rh-Hr Blood-Group System; D001963:Bromelains