Chemical selection of alcohol dehydrogenase negative mutants in drosophila.
We describe a selection procedure which utilizes the vapor from an unsaturated alcohol, 1-pentene-3-ol, for the detection and isolation of mutant flies with little or no alcohol dehydrogenase activity. ADH-negative flies are unaffected by exposure to the unsaturated alcohol, but ADH positives (wild-types) die after short exposure. The technique can be used to select rare ADH-negative individuals from large populations of wild-type flies.
D000367:Age Factors; D000429:Alcohol Oxidoreductases / Q000032:analysis / Q000378:metabolism*; D000475:Alkenes; D000818:Animals; D004330:Drosophila / Q000201:enzymology* / Q000378:metabolism; D008722:Methods; D009154:Mutation; D000439:Pentanols
D000475:Alkenes; D000439:Pentanols; D000429:Alcohol Oxidoreductases