Ultrahigh-temperature activation of a low-temperature Bacillus subtilis spore germination system.
Exposure of Bacillus subtilis A spores to 115 to 125 C for several seconds activated a low-temperature germination system that remained dormant after a heat treatment that activated the normal l-alanine- and glucose-stimulated germination systems. The low-temperature germination system was characterized by an optimum temperature lower than that of the l-alanine or glucose germination systems (30 C versus 45 C), germination in the absence of exogenous germination stimulants, and the capacity for heat-induced deactivation and subsequent reactivation. The rates of activation at 115 to 125 C were exponential and were not influenced by a previous heat treatment that activated the l-alanine- or glucose-stimulated germination systems. Although activation of the low-temperature germination system was accompanied by suppression of l-alanine-stimulated germination, it did not appear to be a modification of the l-alanine germination system.
D000409:Alanine; D001412:Bacillus subtilis / Q000254:growth & development*; D002470:Cell Survival; D003470:Culture Media; D005947:Glucose; D006358:Hot Temperature; D010710:Phosphates; D010848:Picolinic Acids; D012964:Sodium; D012965:Sodium Chloride; D013171:Spores, Bacterial / Q000254:growth & development; D013237:Stereoisomerism; D013696:Temperature; D013997:Time Factors
D003470:Culture Media; D010710:Phosphates; D010848:Picolinic Acids; D012965:Sodium Chloride; D012964:Sodium; D005947:Glucose; D000409:Alanine