The inactivation of bradykinin in the pulmonary circulation of isolated lungs.
1. The isolated lungs of guinea-pigs, rats and dogs, perfused with Krebs bicarbonate solution via the pulmonary artery, inactivated 95, 98 and 99% respectively of the bradykinin infused through them.2. This inactivation process was not inhibited by 2-mercaptoethanol (1 mM) or EDTA (500 muM).3. The inactivation was inhibited by 2:3-dimercaptopropanol (100 muM), N-ethylmaleimide (100 muM), and several of the bradykinin potentiating peptides from Bothrops jararaca, in both natural and synthetic forms.4. The effect of these compounds, 2-mercaptoethanol, EDTA, 2:3-dimercaptopropanol, N-ethylmaleimide and Bothrops peptides on the pulmonary bradykininase activity is the same as their effect on pulmonary angiotensin I converting enzyme.
D000818:Animals; D001920:Bradykinin / Q000378:metabolism*; D002952:Citric Acid Cycle; D004112:Dimercaprol / Q000494:pharmacology; D004285:Dogs; D004357:Drug Synergism; D004492:Edetic Acid / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008168:Lung / Q000378:metabolism* / Q000502:physiology; D008623:Mercaptoethanol / Q000494:pharmacology; D011652:Pulmonary Circulation; D051381:Rats
D004112:Dimercaprol; D008623:Mercaptoethanol; D004492:Edetic Acid; D005033:Ethylmaleimide; D001920:Bradykinin