Validation of a "clearing" assay for milk lipoprotein lipase in agarose gel.
We have developed a simplified method for the quantitative assay of lipoprotein lipase in cow's milk based on the hydrolysis of a glyceride emulsion in semisolid agarose gel. The area of clearing produced thereby is a function of enzyme concentration. Absolute molar rates for unknown samples may be calculated if standards of known activity are used concurrently. The precision of the simplified assay compared favorably with a method based on titrimetric determination of the rate of free fatty acid release. A modified assay has been used to assess the potency of lipoproteins in lipoprotein lipase activation.
D000818:Animals; D002417:Cattle; D005260:Female; D006868:Hydrolysis; D005779:Immunodiffusion; D008071:Lipoprotein Lipase / Q000302:isolation & purification*; D008722:Methods; D008892:Milk / Q000032:analysis / Q000201:enzymology*
D008071:Lipoprotein Lipase