Activity of deoxyribonucleic acid fragments of defined size in Bacillus subtilis transformation.
The transforming activity of Bacillus subtilis deoxyribonucleic acid (DNA) that had been sheared and purified with respect to size by sucrose gradient sedimentation is given as a function of the DNA molecular weight. It is shown (i) that fragments of median molecular weight 1.2 x 10(6) have finite activity (10(-4)), (ii) that the shape of the activity-versus-molecular weight function is qualitatively similar to that observed previously for Diplococcus pneumoniae, and (iii) that this shape precludes interpretation in terms of critical size models.
D001412:Bacillus subtilis / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D004269:DNA, Bacterial / Q000302:isolation & purification / Q000378:metabolism*; D003851:Deoxyribonucleases; D008970:Molecular Weight; D013395:Sucrose; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D013395:Sucrose; D003851:Deoxyribonucleases