title: Comparative studies on Salmonella isolations from feeds in ten laboratories.
abstract: Pig feed samples, artificially and naturally contaminated with S. typhimurium, were distributed to ten laboratories. The isolation rates obtained by using a standardized pre-enrichment method and the laboratories' "own" methods were compared. In nearly all laboratories the standardized pre-enrichment method gave better results. Extending the duration of incubation of the enrichment medium to 48 h followed by a second subculture improved the isolation rates. In general, a pre-enrichment method should be used for the isolation of salmonellae, especially when they are injured or small numbers of them are expected in the material to be investigated.
mesh_terms: D000821:Animal Feed; D001431:Bacteriological Techniques / Q000592:standards; D002452:Cell Count; D005516:Food Microbiology; D012475:Salmonella / Q000302:isolation & purification*; D012486:Salmonella typhimurium / Q000302:isolation & purification; D012703:Serotyping