title: Subcellular localization of isoleucine-valine biosynthetic enzymes in yeast.
abstract: By using the method of stepwise homogenization of yeast spheroplast lysates employed previously with the leucine biosynthetic enzymes, it is shown that threonine deaminase, acetohydroxy acid synthase, Mg(2+)-dependent isomero-reductase, and dihydroxy acid dehydratase are particulate. Density gradient centrifugation and the behavior of marker enzymes suggest that all of the above enzymes of the isoleucine-valine biosynthetic pathway are associated with the mitochondria.
mesh_terms: D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D006836:Hydro-Lyases / Q000302:isolation & purification; D007532:Isoleucine / Q000096:biosynthesis*; D007535:Isomerases / Q000302:isolation & purification; D007651:Keto Acids; D008928:Mitochondria / Q000201:enzymology; D010088:Oxidoreductases / Q000302:isolation & purification; D007652:Oxo-Acid-Lyases / Q000302:isolation & purification; D011773:Pyruvates; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D013104:Spheroplasts; D013395:Sucrose; D013912:Threonine; D013997:Time Factors; D014050:Toluene / Q000494:pharmacology; D014631:Valerates; D014633:Valine / Q000096:biosynthesis*
chemical_list: D007651:Keto Acids; D011773:Pyruvates; D014631:Valerates; D007532:Isoleucine; D013912:Threonine; D014050:Toluene; D013395:Sucrose; D010088:Oxidoreductases; D007652:Oxo-Acid-Lyases; D006836:Hydro-Lyases; D007535:Isomerases; D014633:Valine