Isolation of Aleutian mink disease virus by affinity chromatography.
Affinity chromatography was used to isolate the Aleutian disease virus of mink. Dissociation of the immunoadsorbent-virus complex with 0.75 molar sodium chloride and then with a glycine-hydrochloride gradient released infective particles resembling picornaviruses. The elution profile suggests that two different types of virus-antibody complexes are formed, one dissociated by sodium chloride and another that requires glycine-hydrochloride in addition to sodium chloride for release of virus.
D000327:Adsorption; D000453:Aleutian Mink Disease / Q000201:enzymology / Q000276:immunology / Q000382:microbiology*; D000818:Animals; D000936:Antigen-Antibody Complex; D000937:Antigen-Antibody Reactions; D000956:Antigens, Viral / Q000032:analysis; D002845:Chromatography; D002846:Chromatography, Affinity; D005998:Glycine; D007074:Immunoglobulin G / Q000032:analysis; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008213:Lymphocyte Activation; D008854:Microscopy, Electron; D009954:Ornithine Carbamoyltransferase / Q000032:analysis; D010759:Phosphorus Isotopes; D012965:Sodium Chloride; D014020:Tissue Extracts / Q000032:analysis; D014713:Viruses, Unclassified / Q000276:immunology / Q000302:isolation & purification*
D000936:Antigen-Antibody Complex; D000956:Antigens, Viral; D007074:Immunoglobulin G; D010759:Phosphorus Isotopes; D014020:Tissue Extracts; D012965:Sodium Chloride; D007770:L-Lactate Dehydrogenase; D009954:Ornithine Carbamoyltransferase; D005998:Glycine