Transformation of germinated spores of Bacillus subtilis on agar plates.
l-Alanine-germinated spores of Bacillus subtilis developed a competence on agar plates after 10 h of incubation. Addition of marker amino acid to the plates was required for the transformation.
D000362:Agar; D000409:Alanine / Q000494:pharmacology; D001412:Bacillus subtilis; D002247:Carbon Isotopes; D003470:Culture Media; D004261:DNA Replication; D004269:DNA, Bacterial / Q000378:metabolism; D013171:Spores, Bacterial; D014170:Transformation, Genetic; D014364:Tryptophan / Q000494:pharmacology
D002247:Carbon Isotopes; D003470:Culture Media; D004269:DNA, Bacterial; D014364:Tryptophan; D000362:Agar; D000409:Alanine