The metabolism of sodium cortisone 21-( 35 S)sulphate in the rat.
1. The metabolism of sodium cortisone 21-[(35)S]sulphate was investigated in rats. 2. Quantitative and qualitative experiments showed that substantial amounts of (35)SO(4) (2-) appeared in the urine of free-ranging rats receiving the ester. 3. Whole-body radioautograms indicated considerable biliary elimination of (35)S and also pointed to the liver as the site of metabolism. 4. When female rats with bile-duct cannulae received sodium cortisone 21-[(35)S]sulphate approx. 70% of the dose appeared in the bile as a doubly conjugated steroid (metabolite I). This metabolite was identified as 3alpha-(beta-d-glucopyranuronosido)- 17alpha-hydroxy-21-[(35)S]sulpho-oxy-5alpha-pregnane-11,20-dione. 5. When metabolite I was administered to a rat with a bile-duct cannula 90% of the dose appeared in the bile unchanged. After the administration (intraperitoneally or orally) of metabolite I to free-ranging rats considerable amounts of (35)SO(4) (2-) appeared in the urine. 6. The route by which (35)SO(4) (2-) might be produced from cortisone [(35)S]sulphate in free-ranging animals is discussed.
D000818:Animals; D001345:Autoradiography; D001646:Bile / Q000378:metabolism; D002854:Chromatography, Paper; D003348:Cortisone / Q000378:metabolism*; D004590:Electrophoresis, Paper; D005260:Female; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D013256:Steroids / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism* / Q000652:urine; D013460:Sulfur Isotopes
D013256:Steroids; D013431:Sulfates; D013460:Sulfur Isotopes; D003348:Cortisone