Inhibition of the thrombin-fibrinogen reaction by a macromolecular factor from rat liver.
1. The supernatant obtained by centrifugation of a rat liver homogenate at 100000g for 1h contained a heat-labile macromolecular inhibitor of the thrombin-fibrinogen reaction. 2. The inhibitor was purified to electrophoretic homogeneity by repeated preparative polyacrylamide disc electrophoresis. Inhibition was observed with purified inhibitor equivalent to about 1mug of protein/ml. 3. The inhibitor had a pI of 3.50-3.75, a molecular weight (from sodium dodecyl sulphate-polyacrylamide-gel electrophoresis) of 72000+/-3000 and was inactivated by p-hydroxymercuribenzoate or 5,5'-dithiobis-(2-nitrobenzoic acid). 4. Kinetic studies revealed a non-competitive inhibition, with the inhibitor probably acting on the thrombin-fibrinogen complex.
D000818:Animals; D001565:Benzoates; D001780:Blood Coagulation Tests; D002850:Chromatography, Gel; D004229:Dithiothreitol; D004589:Electrophoresis, Disc; D004591:Electrophoresis, Polyacrylamide Gel; D005340:Fibrinogen; D006493:Heparin; D006902:Hydroxymercuribenzoates; D007700:Kinetics; D008099:Liver / Q000032:analysis*; D008297:Male; D008970:Molecular Weight; D051381:Rats; D013440:Sulfides; D013917:Thrombin / Q000037:antagonists & inhibitors*; D014020:Tissue Extracts / Q000494:pharmacology*; D014361:Trypsin Inhibitors
D001565:Benzoates; D006902:Hydroxymercuribenzoates; D013440:Sulfides; D014020:Tissue Extracts; D014361:Trypsin Inhibitors; D005340:Fibrinogen; D006493:Heparin; D013917:Thrombin; D004229:Dithiothreitol