Mycoplasmacidal activity of peroxidase-H2O2-halide systems.
A mycoplasmacidal system consisting of myeloperoxidase (MPO)-containing granules, H(2)O(2), and a halide is described. In all parameters measured, it appears to be identical to the MPO-H(2)O(2)-halide bactericidal system previously reported. It has a pH optimum of approximately 5.5 and an optimal MPO:H(2)O(2) ratio of 1:25. The halide requirement can be satisfied by either chloride or iodide. Through the use of taurine or horseradish peroxidase substitution, chloride-mediated killing can be distinguished from iodide-mediated killing. The relationship of this mycoplasmacidal system to other mycoplasmacidal systems and to host surveillance of mycoplasma is discussed.
D000818:Animals; D002712:Chlorides / Q000494:pharmacology*; D004355:Drug Stability; D006139:Guaiacol / Q000494:pharmacology; D006168:Guinea Pigs; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007454:Iodides / Q000494:pharmacology*; D007962:Leukocytes / Q000201:enzymology; D009174:Mycoplasma / Q000187:drug effects*; D010544:Peroxidases / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D010944:Plants / Q000201:enzymology
D002712:Chlorides; D007454:Iodides; D006139:Guaiacol; D006861:Hydrogen Peroxide; D010544:Peroxidases