title: Virus-specific ribonucleic acid in the nucleus and cytoplasm of rat embryo cells transformed by adenovirus type 2.
abstract: Nuclei were isolated from rat embryo cells transformed by adenovirus type 2. Nuclear and cytoplasmic virus-specific ribonucleic acids (RNA) were characterized and quantitated by deoxyribonucleic acid (DNA)-RNA hybrid formation with adenovirus DNA. The results indicate that most, if not all, virus-specific RNA molecules are synthesized in the cell nucleus and subsequently transported into cytoplasm where they degrade with a half-life of 1 to 2 hr. No difference in base sequences between nuclear and cytoplasmic virus-specific RNA species can be detected by hybridization competition experiment with viral DNA.
mesh_terms: D000256:Adenoviridae / Q000032:analysis* / Q000254:growth & development / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002277:Carcinoma; D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis* / Q000378:metabolism; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D003593:Cytoplasm / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D007822:Laryngeal Neoplasms; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats / Q000196:embryology; D012703:Serotyping; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014529:Uridine / Q000378:metabolism
chemical_list: D012367:RNA, Viral; D014316:Tritium; D013936:Thymidine; D014529:Uridine