Two benzaldehyde dehydrogenases in bacterium N.C.I.B. 8250. Distinguishing properties and regulation.
Evidence is presented for the existence in bacterium N.C.I.B. 8250 of two inducible NAD(+)-linked benzaldehyde dehydrogenases. They may be distinguished in crude extracts by their different thermal stabilities at high pH values, benzaldehyde dehydrogenase I being much more heat-stable than benzaldehyde dehydrogenase II. Only benzaldehyde dehydrogenase I is activated by K(+) and certain other univalent cations. Gel-filtration experiments indicate that both enzymes have molecular weights of about 180000. Both enzymes are induced by growth on l-mandelate or phenylglyoxylate; only benzaldehyde dehydrogenase I is gratuitously induced by thiophenoxyacetate and only benzaldehyde dehydrogenase II is induced by benzyl alcohol, by benzaldehyde, and by a number of heterocyclic compounds which do not support growth. Mutants have been isolated that lack either benzaldehyde dehydrogenase II or benzyl alcohol dehydrogenase, or both of the enzymes. Results obtained in induction experiments with the wild-type bacterium N.C.I.B. 8250 and with the mutants show that benzaldehyde dehydrogenase II and benzyl alcohol dehydrogenase are co-ordinately regulated. Overall, the results suggest that benzaldehyde dehydrogenase I is associated with the metabolism of l-mandelate whereas benzaldehyde dehydrogenase II is associated with the metabolism of benzyl alcohol.
D000421:Alcaligenes / Q000201:enzymology*; D000438:Alcohols / Q000378:metabolism / Q000494:pharmacology; D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D001547:Benzaldehydes / Q000494:pharmacology; D001593:Benzyl Compounds / Q000378:metabolism / Q000494:pharmacology; D002850:Chromatography, Gel; D004789:Enzyme Activation; D004790:Enzyme Induction; D006038:Glyoxylates / Q000494:pharmacology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008333:Mandelic Acids / Q000378:metabolism / Q000494:pharmacology; D008970:Molecular Weight; D009154:Mutation; D011188:Potassium; D013864:Thioglycolates / Q000494:pharmacology; D014465:Ultrasonics
D000438:Alcohols; D001547:Benzaldehydes; D001593:Benzyl Compounds; D006038:Glyoxylates; D008333:Mandelic Acids; D013864:Thioglycolates; D000445:Aldehyde Oxidoreductases; D011188:Potassium