A new bacterial sphingophospholipid containing 3-aminopropane-1,2-diol.
1. A new sphingophospholipid has been isolated from the bacterial fraction of sheep rumen contents. 2. This new lipid has been characterized as a ceramide phosphate moiety esterified to 3-aminopropane-1,2-diol through the primary alcohol group. 3. Mass spectrometry has shown the intact lipid to contain a vicinal hydroxyl amino grouping, and oxidation with periodate converts it quantitatively into a new phospholipid which is probably ceramide phosphorylglycolaldehyde. 4. The sphingophospholipid contains a mixture of saturated long-chain bases mainly with branched-chain alkyl groups, which are typical of a microbial origin. 5. A Gram-negative bacterium isolated from rumen contents contains about 30% of the new lipid in its phospholipids when grown in pure culture.
D000433:1-Propanol / Q000032:analysis*; D000596:Amino Acids / Q000032:analysis; D000605:Amino Alcohols / Q000032:analysis*; D000818:Animals; D001419:Bacteria / Q000032:analysis*; D001483:Base Sequence; D002518:Ceramides / Q000032:analysis; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis; D013058:Mass Spectrometry; D012417:Rumen / Q000382:microbiology; D012756:Sheep; D013107:Sphingolipids / Q000032:analysis*
D000596:Amino Acids; D000605:Amino Alcohols; D002518:Ceramides; D005227:Fatty Acids; D013107:Sphingolipids; D000433:1-Propanol