Analytical method for streptothricin-type antibiotics: structure of antibiotic LL-BL136.
An analytical procedure was devised which can distinguish members of the streptothricin family of antibiotics. It is based upon an analysis of the hydrolysis products of the antibiotics using a Technicon amino acid autoanalyzer under special conditions. The various fragments including the different streptolidine-amino sugar compounds were well resolved. A basic water-soluble antibiotic discovered in our laboratories and named LL-BL136 was compared to other members of this group by this technique. It was not differentiated from the antibiotic SF-701 reported by Tsuruoka. The autoanalyzer results along with other physicochemical data permitted a structure proposal for this antibiotic, which is the N-methyl-desformimino derivative of antibiotic LL-AC541.
D000617:Aminoglycosides / Q000032:analysis; D000900:Anti-Bacterial Agents / Q000032:analysis*; D001322:Autoanalysis; D055598:Chemical Phenomena; D002621:Chemistry; D002854:Chromatography, Paper; D005285:Fermentation; D008722:Methods; D010455:Peptides / Q000032:analysis
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D010455:Peptides