Molecular characteristics of chicken liver arginase.
A purification procedure for the preparation of chicken liver arginase in a homogeneous form is presented. The enzyme hydrolyses both arginine and argininic acid. Kinetic analysis reveals that the enzyme binds arginine when the amino group is protonated or unprotonated; however, the unprotonated form seems to be hydrolysed more rapidly. The enzyme exchanges with the medium approx. 1.6Mn(2+) ions per molecule.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001119:Arginase / Q000302:isolation & purification*; D001120:Arginine / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D002645:Chickens; D004589:Electrophoresis, Disc; D006868:Hydrolysis; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008345:Manganese; D008722:Methods; D011506:Proteins / Q000032:analysis; D011868:Radioisotopes
D000596:Amino Acids; D011506:Proteins; D011868:Radioisotopes; D008345:Manganese; D001120:Arginine; D001119:Arginase