Inhibition of Sindbis virus release by media of low ionic strength: an electron microscope study.
Release of Sindbis virus from infected cells is inhibited by lowering the ionic strength of the medium. To determine the nature of the inhibited step, we examined, by electron microscopy, both freeze-etched and thin-sectioned preparations which had been fixed with either glutaraldehyde or formaldehyde. Inhibitory medium had two different effects on Sindbis virus release: virus budding was partially inhibited, and those virions which did mature were precipitated on the surface of the cell. Freeze-etched, inhibited cells showed very few viral buds. After shift to normal medium, the number of budding virions increased dramatically, far exceeding the quantity found in normal controls. Thus, low ionic strength medium clearly inhibited an early stage of virus maturation. The results were the same regardless of the fixative. Thin sections of glutaraldehyde-fixed, inhibited cells contained large extracellular aggregates of mature virus which were not present in similar, formaldehyde-fixed preparations. Fixation of radioactively-labeled, inhibited cultures revealed that approximately half of the virus that could be released from inhibited cells by raising the ionic strength of the medium could also be released by formaldehyde, but not by glutaraldehyde. This fraction probably represents mature virus attached to the cell surface by the ionic conditions.
D000447:Aldehydes; D000818:Animals; D002462:Cell Membrane / Q000382:microbiology; D002478:Cells, Cultured / Q000382:microbiology; D002642:Chick Embryo; D003470:Culture Media; D005069:Evaluation Studies as Topic; D005347:Fibroblasts; D005557:Formaldehyde; D005613:Freeze Etching; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D008867:Microtomy; D012845:Sindbis Virus / Q000254:growth & development* / Q000302:isolation & purification; D014316:Tritium; D014529:Uridine
D000447:Aldehydes; D003470:Culture Media; D014316:Tritium; D005557:Formaldehyde; D014529:Uridine