title: Polyamine biogenesis in the rat mammary gland during pregnancy and lactation.
abstract: Polyamines and RNA accumulate in the rat mammary gland during pregnancy, but the major increases occur after parturition. Therefore the major increases occur after the gland has obtained its maximal complement of epithelial cells. During lactation, the spermidine concentration rises above 5mm and RNA content in the lactating mammary gland reaches a value 16 times that of the unstimulated mammary gland. The ratio of spermidine/spermine, an increase of which initially signals an elevation in biosynthetic activity, is near 1 in the normal mammary gland and is greater than 10 in the lactating mammary gland. Putrescine concentration is very low during the entire course of mammary-gland development, with the exception of early pregnancy. The low putrescine concentration probably reflects the very rapid conversion of putrescine into spermidine. Both ornithine decarboxylase, the enzyme that synthesizes putrescine, and putrescine-stimulated S-adenosyl-l-methionine decarboxylase, the enzyme that synthesizes spermidine, increase in activity during middle and late pregnancy; during lactation, both enzyme activities are elevated until the 21st day of lactation, and then decline. These declines are concomitant with involution. Also, it was found that the amount of ribonuclease activity in the mammary gland was very high during lactation, almost double that in the gland during pregnancy.
mesh_terms: D000818:Animals; D002247:Carbon Isotopes; D002262:Carboxy-Lyases / Q000032:analysis; D004847:Epithelial Cells; D004848:Epithelium / Q000032:analysis; D005260:Female; D007774:Lactation; D008321:Mammary Glands, Animal / Q000201:enzymology / Q000378:metabolism*; D009952:Ornithine / Q000032:analysis; D011073:Polyamines / Q000096:biosynthesis*; D011247:Pregnancy; D011270:Pregnancy, Animal; D011700:Putrescine / Q000032:analysis / Q000096:biosynthesis; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D012260:Ribonucleases / Q000032:analysis; D013095:Spermidine / Q000032:analysis / Q000096:biosynthesis; D013096:Spermine / Q000032:analysis
chemical_list: D002247:Carbon Isotopes; D011073:Polyamines; D013096:Spermine; D012313:RNA; D009952:Ornithine; D012260:Ribonucleases; D002262:Carboxy-Lyases; D013095:Spermidine; D011700:Putrescine