Virus-specific ribonucleic acid in the nucleus and cytoplasm of rat embryo cells transformed by adenovirus type 2.
Nuclei were isolated from rat embryo cells transformed by adenovirus type 2. Nuclear and cytoplasmic virus-specific ribonucleic acids (RNA) were characterized and quantitated by deoxyribonucleic acid (DNA)-RNA hybrid formation with adenovirus DNA. The results indicate that most, if not all, virus-specific RNA molecules are synthesized in the cell nucleus and subsequently transported into cytoplasm where they degrade with a half-life of 1 to 2 hr. No difference in base sequences between nuclear and cytoplasmic virus-specific RNA species can be detected by hybridization competition experiment with viral DNA.
D000256:Adenoviridae / Q000032:analysis* / Q000254:growth & development / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002277:Carcinoma; D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis* / Q000378:metabolism; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D003593:Cytoplasm / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D007822:Laryngeal Neoplasms; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats / Q000196:embryology; D012703:Serotyping; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D012367:RNA, Viral; D014316:Tritium; D013936:Thymidine; D014529:Uridine