title: 6-phosphogluconate dehydrogenase and the assay of uridine diphosphate glucose pyrophosphorylase in the cellular slime mould Dictyostelium discoideum.
abstract: 1. 6-Phosphogluconate dehydrogenase activity is present in all morphogenetic stages during cell differentiation in the cellular slime mould. 2. The different ratios of 6-phosphogluconate dehydrogenase/UDP-glucose pyrophosphorylase observed during this process can render spectrophotometric assays of UDP-glucose pyrophosphorylase inaccurate. 3. The disputed occurrence of increases in specific activity of UDP-glucose pyrophosphorylase during cell differentiation in the cellular slime mould is discussed in the light of these observations.
mesh_terms: D002454:Cell Differentiation; D002474:Cell-Free System; D009235:Myxomycetes / Q000201:enzymology*; D009713:Nucleotidyltransferases / Q000032:analysis*; D010734:Phosphogluconate Dehydrogenase / Q000032:analysis*; D013053:Spectrophotometry; D013172:Spores, Fungal / Q000201:enzymology
chemical_list: D010734:Phosphogluconate Dehydrogenase; D009713:Nucleotidyltransferases