title: Implantation of exogenous enzymatic activity in isolated alveolar macrophages.
abstract: Alveolar macrophages acquired the capacity to oxidize uric acid after exposure to uricase. Presumably this resulted from intracellular incorporation of the enzyme by endocytosis. This approach may provide a general method for (i) testing regulatory biochemical mechanisms, (ii) therapeutic replacement of deficient regulatory macromolecules, and (iii) studying environmental factors that produce genetic alterations.
mesh_terms: D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D004705:Endocytosis; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism / Q000502:physiology; D011650:Pulmonary Alveoli / Q000166:cytology; D011817:Rabbits; D014503:Urate Oxidase / Q000378:metabolism*; D014527:Uric Acid / Q000378:metabolism
chemical_list: D014527:Uric Acid; D000968:Antimycin A; D014503:Urate Oxidase