The identification of a lipid-mobilizing factor from sheep midbrain.
The mass spectrum of a lipolytic substance from purified sheep midbrain extract indicated that it was isoprenaline, not N-n-propylnoradrenaline, noradrenaline or adrenaline. The identification of the lipid-mobilizing factor as isoprenaline was confirmed qualitatively by t.l.c. and g.l.c. and quantitatively by determination of its lipolytic activity and u.v. absorption and by fluorimetric determination of its catecholamine content.
D000273:Adipose Tissue / Q000187:drug effects; D000818:Animals; D001681:Biological Assay; D001794:Blood Pressure / Q000187:drug effects; D002395:Catecholamines / Q000032:analysis; D002845:Chromatography; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D005470:Fluorometry; D006339:Heart Rate / Q000187:drug effects; D007545:Isoproterenol / Q000032:analysis* / Q000494:pharmacology; D008053:Lipid Mobilization; D008297:Male; D013058:Mass Spectrometry; D008636:Mesencephalon / Q000032:analysis*; D051381:Rats; D012756:Sheep; D013053:Spectrophotometry
D002395:Catecholamines; D007545:Isoproterenol