Effect of compounds of the urea-guanidinium class on renaturation and thermal stability of acid-soluble collagen.
The effects of guanidinium salts in decreasing the renaturation rate and lowering the thermal stability of acid-soluble calf-skin collagen have been compared with those of formamide and urea. With the exception of guanidinium sulphate at higher concentrations, no qualitative differences were apparent in the effects of these perturbants, which thus differed only in molar activity. Activity variation in the guanidinium salts reflected a net effect resulting from additivity of cation and anion contributions. As observed in other protein systems, lyotropic activity increased in the series formamide<urea<guanidinium ion, and in the guanidinium salts in the anion order fluoride<sulphate<chloride<bromide<nitrate<iodide. Low activities of guanidinium fluoride and sulphate were attributable to counter-effects of the anions, which acted as structural stabilizers. Changes in renaturation kinetics induced by either temperature or added perturbants appeared to conform with the Flory-Weaver model for the collagen transition. Additivity and non-specificity of the observed effects are discussed with particular reference to a common mechanism involving weak, non-saturated binding of perturbants at protein peptide groups.
D000818:Animals; D001965:Bromides; D002417:Cattle; D002712:Chlorides; D003094:Collagen; D004355:Drug Stability; D005459:Fluorides; D005559:Formamides; D006146:Guanidines; D006358:Hot Temperature; D007454:Iodides; D007700:Kinetics; D009566:Nitrates; D009904:Optical Rotation; D011489:Protein Denaturation; D013431:Sulfates; D014508:Urea; D014783:Viscosity
D001965:Bromides; D002712:Chlorides; D005559:Formamides; D006146:Guanidines; D007454:Iodides; D009566:Nitrates; D013431:Sulfates; D014508:Urea; D003094:Collagen; D005459:Fluorides