title: The development of the respiratory chain of Saccharomyces carlsbergensis during respiratory adaptation.
abstract: 1. Subcellular fractionation of sphaeroplasts produced at different stages during the first 4h of respiratory adaptation of anaerobically grown glucose-de-repressed Saccharomyces carlsbergensis gave mitochondrial fractions that contained all the detectable c- and a-type cytochromes. 2. The rates of cytochrome formation were studied; individual cytochromes were produced at different rates so as to give respiratory chains having widely differing cytochrome ratios. A CO-reacting haemoprotein other than cytochrome a(3) also increased throughout 8h of respiratory adaptation. 3. Even after short periods of aeration, organisms contained mitochondria in which cytochrome-cytochrome interactions and the reaction of cytochrome a(3) with O(2) proceeded at rates almost as fast as in organelles from aerobically grown cells. 4. The technique of flow-flash photolysis enabled kinetic resolution of the reoxidation of cytochromes a(3) and a to be achieved and their individual contributions to extinction changes in the Soret region were assessed. The ratio cytochrome a(3)/cytochrome a increased over the early stages of adaptation.
mesh_terms: D000222:Adaptation, Physiological; D000693:Anaerobiosis; D002458:Cell Fractionation; D003580:Cytochromes / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism; D009940:Organoids / Q000378:metabolism; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D010782:Photolysis; D012440:Saccharomyces / Q000378:metabolism*; D013997:Time Factors
chemical_list: D003580:Cytochromes; D005947:Glucose