Replication of the parvovirus MVM. I. Dependence of virus multiplication and plaque formation on cell growth.
A plaque assay has been developed for the minute virus of mice. The infectious unit is a single particle. Plaque size is determined by the extent of cell division in the infected monolayer. Infection of quiescent and serum-stimulated cells suggests that virus multiplication depends on host function(s) that are not normally expressed in resting cells.
D000818:Animals; D002417:Cattle / Q000276:immunology; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D004247:DNA / Q000096:biosynthesis; D004267:DNA Viruses / Q000254:growth & development* / Q000302:isolation & purification; D005347:Fibroblasts; D007106:Immune Sera / Q000494:pharmacology; D007668:Kidney; D051379:Mice; D051381:Rats; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D010948:Viral Plaque Assay; D014776:Virus Cultivation; D014779:Virus Replication
D007106:Immune Sera; D014316:Tritium; D004247:DNA; D013936:Thymidine