Structural proteins of Pichinde virus.
Pichinde virus, a member of the arenovirus group, was found to have four polypeptides by polyacrylamide gel electrophoresis. Two components, V(I) and V(II), had molecular weights of about 72,000, whereas V(III) had a molecular weight of 34,000. A minor component, V(IV), had a molecular weight of about 12,000. Glucosamine was incorporated into V(II) and V(III), suggesting that these components were glycopeptides whereas V(I) and V(IV) were polypeptides. Treatment of the virus with Nonidet P-40 removed V(III), but V(I) and V(II) remained associated with the virus nucleic acid. This suggests a functional role of a ribonucleoprotein for V(I) and an envelope glycoprotein for V(III). V(II), the major glycopeptide, could function both as a membrane component and as a nucleoprotein.
D000596:Amino Acids; D000818:Animals; D002247:Carbon Isotopes; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D005944:Glucosamine; D006018:Glycols; D006020:Glycopeptides / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis; D007525:Isoelectric Focusing; D007668:Kidney; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D012328:RNA Viruses / Q000032:analysis* / Q000254:growth & development / Q000302:isolation & purification; D013501:Surface-Active Agents; D014316:Tritium; D014529:Uridine; D014764:Viral Proteins / Q000032:analysis*
D000596:Amino Acids; D002247:Carbon Isotopes; D006018:Glycols; D006020:Glycopeptides; D006023:Glycoproteins; D010455:Peptides; D013501:Surface-Active Agents; D014764:Viral Proteins; D014316:Tritium; D005944:Glucosamine; D014529:Uridine