Microsomal palmitoyl coenzyme A synthetase from rat liver. Partial and exchange reactions.
The partial and exchange reactions of long-chain fatty acid activation were determined by using purified microsomal long-chain fatty acyl-CoA synthetase (EC 6.2.1.3). No significant ATP formation from palmitoyl-AMP and PP(i), nor palmitoyl-AMP-dependent CoA disappearance could be demonstrated. Similarly, no palmitate-dependent [(32)P(2)]PP(i)-ATP exchange was catalysed by the pure enzyme. The above partial and exchange reactions were, however, catalysed by the parent microsomal fraction at a rate similar to that of the overall reaction. The implications of these results are discussed.
D000249:Adenosine Monophosphate; D000255:Adenosine Triphosphate; D000818:Animals; D002247:Carbon Isotopes; D002850:Chromatography, Gel; D003066:Coenzyme A Ligases / Q000302:isolation & purification; D003902:Detergents / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology; D007700:Kinetics; D008862:Microsomes, Liver / Q000201:enzymology*; D010169:Palmitic Acids / Q000494:pharmacology; D010759:Phosphorus Isotopes; D051381:Rats; D013696:Temperature; D014316:Tritium
D002247:Carbon Isotopes; D003902:Detergents; D010169:Palmitic Acids; D010759:Phosphorus Isotopes; D014316:Tritium; D000249:Adenosine Monophosphate; D000255:Adenosine Triphosphate; D003066:Coenzyme A Ligases; D005978:Glutathione