Regulation of the enzymes converting L-mandelate into benzoate in bacterium N.C.I.B. 8250.
l-Mandelate is oxidized to benzoate by the enzymes l-mandelate dehydrogenase, phenylglyoxylate carboxy-lyase and benzaldehyde dehydrogenase I. Conditions have been established for measuring these three enzymes as well as benzyl alcohol dehydrogenase, benzaldehyde dehydrogenase II and catechol 1,2-oxygenase in a single cell-free extract prepared from bacterium N.C.I.B. 8250. The kinetics of induction of all these enzymes have been measured under a variety of conditions. l-Mandelate dehydrogenase, phenylglyoxylate carboxy-lyase and benzaldehyde dehydrogenase I appear to be co-ordinately regulated because (a) their differential rates of synthesis are proportional to one another under various conditions of induction and repression, (b) they are specifically and gratuitously induced by thiophenoxyacetate and a number of other compounds, and (c) mutant strains have been isolated that lack all three enzymes. Phenylglyoxylate is the primary inducer of the regulon as mutant strains lacking phenylglyoxylate carboxy-lyase form the other two enzymes in the presence of l-mandelate or phenylglyoxylate, whereas in mutant strains devoid of l-mandelate dehydrogenase activity only phenylglyoxylate induces phenylglyoxylate carboxy-lyase and benzaldehyde dehydrogenase I.
D000421:Alcaligenes / Q000201:enzymology*; D000429:Alcohol Oxidoreductases / Q000096:biosynthesis / Q000378:metabolism; D000445:Aldehyde Oxidoreductases / Q000096:biosynthesis / Q000378:metabolism; D001565:Benzoates / Q000378:metabolism*; D001593:Benzyl Compounds; D002262:Carboxy-Lyases / Q000096:biosynthesis / Q000378:metabolism; D002396:Catechols; D002474:Cell-Free System; D004790:Enzyme Induction; D004794:Enzyme Repression; D006038:Glyoxylates / Q000378:metabolism; D007700:Kinetics; D008333:Mandelic Acids / Q000378:metabolism*; D009154:Mutation; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000096:biosynthesis / Q000378:metabolism; D010105:Oxygenases / Q000096:biosynthesis / Q000378:metabolism; D013864:Thioglycolates / Q000494:pharmacology
D001565:Benzoates; D001593:Benzyl Compounds; D002396:Catechols; D006038:Glyoxylates; D008333:Mandelic Acids; D013864:Thioglycolates; D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; D010105:Oxygenases; D000445:Aldehyde Oxidoreductases; D002262:Carboxy-Lyases