Partial purification and properties of the pyruvate-uridine diphospho-N-acetylglucosamine transferase from Staphylococcus epidermidis.
A method for the partial purification of the pyruvate-uridine-diphospho-N-acetylglucosamine transferase from Staphylococcus epidermidis is presented. Some properties of the enzyme are discussed.
D002130:Calcium Phosphates; D002247:Carbon Isotopes; D002474:Cell-Free System; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D004229:Dithiothreitol; D006595:Hexosamines / Q000096:biosynthesis; D006863:Hydrogen-Ion Concentration; D008722:Methods; D010728:Phosphoenolpyruvate / Q000378:metabolism; D011479:Protamines; D011773:Pyruvates; D013210:Staphylococcus / Q000201:enzymology*; D013431:Sulfates; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D013696:Temperature; D014166:Transferases / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D014461:Ultracentrifugation
D002130:Calcium Phosphates; D002247:Carbon Isotopes; D006595:Hexosamines; D011479:Protamines; D011773:Pyruvates; D013431:Sulfates; D013439:Sulfhydryl Reagents; D010728:Phosphoenolpyruvate; D014166:Transferases; D004229:Dithiothreitol