title: Physiological role of pyruvate carboxylase in a thermophilic bacillus.
abstract: A prototrophic, thermophilic bacillus is in a state of biotin insufficiency when grown in medium consisting of inorganic salts and a carbon source. The effect of this biotin deficiency on the growth rate is severe only if the functioning of pyruvate carboxylase is essential for the utilization of the particular growth substrate. A mutant, PC2, of the thermophile devoid of active pyruvate carboxylase has been isolated. The properties of this mutant confirm the anaplerotic role of this enzyme in the utilization for growth of compounds like glucose and lactate which are catabolized via pyruvate. This conclusion is supported by the finding that revertants isolated from strain PC2 have regained simultaneously the ability to synthesize active pyruvate carboxylase and the ability to utilize glucose or lactate for growth. The growth of mutant PC2 on acetate, unlike that of the parent wild type, is inhibited when glucose or lactate is added to the medium. Secondary mutants obtained from PC2, which are resistant to such inhibition, still carry the original pyruvate carboxylase lesion but are derepressed for isocitrate lyase. This suggests that the inhibition of the growth of mutant PC2 is due to a block in the functioning of the glyoxylate cycle, produced by the glucose or lactate supplement.
mesh_terms: D000085:Acetates / Q000378:metabolism; D001224:Aspartic Acid / Q000378:metabolism; D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D001710:Biotin / Q000378:metabolism / Q000494:pharmacology; D002474:Cell-Free System; D003470:Culture Media; D004794:Enzyme Repression; D005947:Glucose / Q000378:metabolism; D006358:Hot Temperature; D007523:Isocitrates; D007773:Lactates / Q000378:metabolism; D008025:Ligases / Q000096:biosynthesis / Q000378:metabolism*; D009154:Mutation; D007652:Oxo-Acid-Lyases / Q000378:metabolism; D011773:Pyruvates; D013237:Stereoisomerism; D013386:Succinates / Q000378:metabolism
chemical_list: D000085:Acetates; D003470:Culture Media; D007523:Isocitrates; D007773:Lactates; D011773:Pyruvates; D013386:Succinates; D001224:Aspartic Acid; D001710:Biotin; D007652:Oxo-Acid-Lyases; D008025:Ligases; D005947:Glucose