title: Competitive inhibition for amino acid uptake by the indigenous microflora of Upper Klamath Lake.
abstract: The uptake of a specific (14)C-amino acid by the heterotrophic microorganisms in the epilimnion of an eutrophic lake was influenced by the presence of other amino acids. The effect of unlabeled serine on (14)C-glycine uptake was shown to be caused by competitive inhibition, which changed the interpretation of the kinetic parameters, the turnover time, T(t), and the sum of a transport constant, (K(t) + (S(n)), and the natural substrate concentration. The maximum velocity of uptake, V(max), is unaffected by the competitive inhibition.
mesh_terms: D000409:Alanine / Q000494:pharmacology; D000596:Amino Acids / Q000378:metabolism*; D001224:Aspartic Acid / Q000378:metabolism / Q000494:pharmacology; D001419:Bacteria / Q000378:metabolism*; D002170:Canada; D002245:Carbon Dioxide / Q000096:biosynthesis; D002247:Carbon Isotopes; D003470:Culture Media; D005618:Fresh Water; D005971:Glutamates / Q000378:metabolism; D005998:Glycine / Q000378:metabolism / Q000494:pharmacology; D007532:Isoleucine / Q000494:pharmacology; D007930:Leucine / Q000378:metabolism / Q000494:pharmacology; D008851:Micropore Filters; D010933:Plankton / Q000378:metabolism*; D012694:Serine / Q000494:pharmacology; D014633:Valine / Q000494:pharmacology; D014871:Water Microbiology
chemical_list: D000596:Amino Acids; D002247:Carbon Isotopes; D003470:Culture Media; D005971:Glutamates; D007532:Isoleucine; D002245:Carbon Dioxide; D001224:Aspartic Acid; D012694:Serine; D007930:Leucine; D014633:Valine; D000409:Alanine; D005998:Glycine