Synthesis and structure of Caulobacter crescentus flagella.
During the normal cell cycle of Caulobacter crescentus, flagella are released into the culture fluid as swarmer cells differentiate into stalked cells. The released flagellum is composed of a filament, hook, and rod. The molecular weight of purified flagellin (subunit of flagella filament) is 25,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The formation of a flagellum opposite the stalk has been observed by microscope during the differentiation of a stalked cell in preparation for cell division. By pulsing synchronized cultures with (14)C-amino acids it has been demonstrated that the synthesis of flagellin occurs approximately 30 to 40 min before cell division. Flagellin, therefore, is synthesized at a discrete time in the cell cycle and is assembled into flagella at a specific site on the cell. A mutant of C. crescentus that fails to synthesize flagellin has been isolated.
D001419:Bacteria / Q000032:analysis / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D002247:Carbon Isotopes; D002455:Cell Division; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D005407:Flagella / Q000032:analysis / Q000378:metabolism; D008854:Microscopy, Electron; D008970:Molecular Weight; D009024:Morphogenesis; D012967:Sodium Dodecyl Sulfate; D013997:Time Factors; D014316:Tritium
D001426:Bacterial Proteins; D002247:Carbon Isotopes; D014316:Tritium; D012967:Sodium Dodecyl Sulfate