The  -chymotrypsin-catalyzed hydrolysis of N-acetyl-L-tryptophan p-nitrophenyl ester in dimethyl sulfoxide at subzero temperatures.
D000085:Acetates; D000215:Acylation; D002384:Catalysis; D002850:Chromatography, Gel; D002918:Chymotrypsin / Q000302:isolation & purification; D002942:Circular Dichroism; D003080:Cold Temperature; D004121:Dimethyl Sulfoxide; D004952:Esters; D006868:Hydrolysis; D009596:Nitrophenols; D009994:Osmolar Concentration; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014364:Tryptophan
D000085:Acetates; D004952:Esters; D009596:Nitrophenols; D014364:Tryptophan; D002918:Chymotrypsin; D004121:Dimethyl Sulfoxide