Production of D-alanine by Corynebacterium fascians.
A strain identified as Corynebacterium fascians was found to accumulate extracellular D-alanine from glycerol. Cultural conditions for the accumulation of D-alanine were investigated and, as a result, a yield of 7 g of D-alanine per liter was obtained after a 96-h incubation in a medium containing 5% glycerol, 4% (NH(4))(2)HPO(4), and 0.3% corn steep liquor. Optical purity of D-alanine was dependent upon the concentration of corn steep liquor. At the optimal condition, almost optically pure D-alanine was formed and readily isolated (5 g/liter) from the fermentation broth. The product was not contaminated with any detectable amount of other amino acids, except for glycine which was present at a concentration of less than 1 percent.
D000409:Alanine / Q000096:biosynthesis* / Q000302:isolation & purification; D000596:Amino Acids / Q000096:biosynthesis; D001419:Bacteria / Q000378:metabolism; D002854:Chromatography, Paper; D003352:Corynebacterium / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism; D010710:Phosphates; D013045:Species Specificity; D013237:Stereoisomerism; D003313:Zea mays
D000596:Amino Acids; D003470:Culture Media; D010710:Phosphates; D000409:Alanine; D005990:Glycerol