Serotonin: radioimmunoassay.
The development of a radioimmunoassay for serotonin is described. Serotonin is made antigenic by coupling it to bovine serum albumin. Thrombocyte-free plasma instead of serum from the immunized animals is used for radioimmunoassay. Less than 1 ng serotonin can be measured by this procedure.
D000818:Animals; D000936:Antigen-Antibody Complex; D001792:Blood Platelets; D008722:Methods; D010949:Plasma; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D012701:Serotonin / Q000032:analysis*; D012710:Serum Albumin, Bovine; D014316:Tritium
D000936:Antigen-Antibody Complex; D014316:Tritium; D012710:Serum Albumin, Bovine; D012701:Serotonin