title: Morphological, biochemical, and growth characteristics of pseudomonas cepacia from distilled water.
abstract: Studies were conducted on three strains of Pseudomonas cepacia isolated and maintained in distilled water and on a laboratory-subcultured strain transferred to distilled water. Optimum growth rates and maximum population yields of the four strains in distilled water were obtained at 37 C, although high population levels (10(6)-10(7)/ml) were reached and maintained over extended incubation periods at temperatures from 18 C to 42 C. Two strains were able to grow in distilled water at temperatures ranging from 12 C to 48 C and to survive 48 h and 21 days at 50 C and 10 C, respectively. Cells from distilled water cultures inoculated into Trypticase soy broth showed an immediate two- to three-log drop at upper and lower temperature limits; survivors were able to initiate logarithmic growth. Results obtained in morphological, biochemical, and antibiotic tests affirmed the strain differences noted in growth studies.
mesh_terms: D000332:Aerobiosis; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D050260:Carbohydrate Metabolism; D002262:Carboxy-Lyases / Q000096:biosynthesis; D002470:Cell Survival; D003470:Culture Media; D004352:Drug Resistance, Microbial; D005407:Flagella; D005780:Gelatin / Q000378:metabolism; D006761:Hospitals; D008239:Lysine; D008826:Microbial Sensitivity Tests; D008854:Microscopy, Electron; D009566:Nitrates / Q000378:metabolism; D011549:Pseudomonas / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D014510:Urease / Q000096:biosynthesis; D014871:Water Microbiology
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D009566:Nitrates; D005780:Gelatin; D014510:Urease; D002262:Carboxy-Lyases; D008239:Lysine