Properties of an antigenic glycoprotein isolated from influenza virus hemagglutinin.
A purified antigen, HABA protein, has been derived from influenza virus concentrates by extraction with denaturing solvents. The protein lacks hemagglutinating activity but binds completely strain-specific, hemagglutination-inhibiting antibodies and induces neutralizing antibodies in experimental animals. Physicochemical characterization of HABA protein identifies it as a single homogeneous glycoprotein with a molecular weight of 78,000. On dissociation with guanidine or sodium dodecyl sulfate, in the presence of reducing agents, only one size of polypeptide with a molecular weight of the order of 40,000 is characteristic of the preparations. The data indicate that HABA protein is a dimer of HA(1) polypeptide of the influenza virus hemagglutinin substructure, and that only trace amounts of other polypeptides are present.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D000956:Antigens, Viral / Q000032:analysis* / Q000302:isolation & purification; D001666:Binding Sites, Antibody; D002845:Chromatography; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis* / Q000302:isolation & purification; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006389:Hemagglutinins, Viral / Q000032:analysis*; D006601:Hexoses / Q000032:analysis; D051379:Mice; D008968:Molecular Conformation; D008970:Molecular Weight; D009975:Orthomyxoviridae / Q000276:immunology*; D010455:Peptides / Q000032:analysis; D011485:Protein Binding; D011489:Protein Denaturation; D014764:Viral Proteins / Q000032:analysis* / Q000302:isolation & purification
D000596:Amino Acids; D000956:Antigens, Viral; D006023:Glycoproteins; D006389:Hemagglutinins, Viral; D006601:Hexoses; D010455:Peptides; D014764:Viral Proteins