Structural organization of murine intracisternal A particles.
Isolated murine intracisternal A particles have a distinctive set of structural properties as compared to the recognized oncogenic ribonucleic acid tumor viruses. A particles consist of two closely approximated concentric shells. The outer shell is largely, but not entirely, susceptible to treatment with detergents such as deoxycholate and Triton X-100. The inner shell is resistant to these agents and is stabilized against solubilization by sodium dodecyl sulfate as a result of disulfide bonding between its constituent proteins. The major A particle structural protein (molecular weight approximately 70,000) is contained in the inner shell. A protein component with a molecular weight of about 80,000 may be preferentially concentrated in sites of firm attachment between the inner and outer shells.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured / Q000166:cytology; D002499:Centrifugation, Density Gradient; D003840:Deoxycholic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007181:Inclusion Bodies, Viral / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D009368:Neoplasm Transplantation; D009858:Oncogenic Viruses / Q000032:analysis / Q000187:drug effects / Q000302:isolation & purification; D010954:Plasmacytoma; D012328:RNA Viruses / Q000032:analysis / Q000187:drug effects / Q000302:isolation & purification; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology; D014764:Viral Proteins / Q000032:analysis
D013501:Surface-Active Agents; D014764:Viral Proteins; D003840:Deoxycholic Acid; D012967:Sodium Dodecyl Sulfate