Production of specific fragments of phi X174 replicative form DNA by a restriction enzyme from Haemophilus parainfluenzae, endonuclease HP.
A restriction endonuclease from Haemophilus parainfluenzae degrades phiX174 replicative form DNA into eight specific fragments, ranging from 1,700 to 150 base pairs and terminated specifically by deoxycytidylic acid.
D000645:Ammonium Sulfate; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003090:Coliphages / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects*; D004267:DNA Viruses / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases / Q000494:pharmacology*; D006190:Haemophilus / Q000201:enzymology*; D009711:Nucleotides / Q000032:analysis; D014316:Tritium
D004279:DNA, Viral; D009711:Nucleotides; D014316:Tritium; D003851:Deoxyribonucleases; D004720:Endonucleases; D000645:Ammonium Sulfate