Alkyl isocyanates as active-site-specific reagents for serine proteases. Identification of the active-site serine as the site of reaction.
D000596:Amino Acids / Q000032:analysis; D001665:Binding Sites; D002219:Carbamates; D002247:Carbon Isotopes; D002852:Chromatography, Ion Exchange; D002918:Chymotrypsin / Q000032:analysis / Q000037:antagonists & inhibitors; D003485:Cyanates; D010450:Endopeptidases; D005561:Formates; D006868:Hydrolysis; D010084:Oxidation-Reduction; D010196:Pancreatic Elastase / Q000032:analysis / Q000037:antagonists & inhibitors; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification; D012694:Serine / Q000032:analysis; D013381:Subtilisins; D014357:Trypsin
D000596:Amino Acids; D002219:Carbamates; D002247:Carbon Isotopes; D003485:Cyanates; D005561:Formates; D010455:Peptides; D012694:Serine; D010450:Endopeptidases; D013381:Subtilisins; D002918:Chymotrypsin; D010196:Pancreatic Elastase; D014357:Trypsin