Lymphocyte transformation induced by autologous cells. II. Stimulation by mitogen-induced lymphoblasts.
Lymphocytes incubated with phytohemagglutinin or concanavalin A develop the capacity to stimulate autologous lymphocyte transformation. This is not attributable to residual mitogen contaminating the lymphoblastic cell preparation as: (a) the dissociation of mitogen from the lymphoblastic cell preparation increases the degree of stimulation observed and (b) the kinetics of lymphocyte transformation stimulated by phytohemagglutinin-induced lymphoblasts is different from that stimulated by phytohemagglutinin. The appearance of the stimulatory determinants on lymphocytes exposed to phytohemagglutinin precedes morphological transformation.
D002478:Cells, Cultured; D002587:Cesium Isotopes; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media; D006801:Humans; D007700:Kinetics; D037102:Lectins / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects* / Q000528:radiation effects; D011830:Radiation Effects; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D002587:Cesium Isotopes; D003470:Culture Media; D037102:Lectins; D014316:Tritium; D003208:Concanavalin A; D013936:Thymidine