title: Creatine phosphokinase activity in rabbit alveolar macrophages.
abstract: An enzyme is present in extracts of rabbit alveolar macrophage which can catalyze the reaction of creatine phosphate with adenosine diphosphate to form adenosine triphosphate and creatine. The enzyme is moderately activated by reduced glutathione, has a pH optimum between pH 6.5 and 7.0, and shows an absolute requirement for Mg(2+). The K(m) for creatine phosphate is approximately 3.6 mm while the K(m) for adenosine diphosphate is about 1.1 mm. The enzyme may play a role in the energy balance of the cell by creating a reserve of energy in the form of creatine phosphate.
mesh_terms: D000818:Animals; D003401:Creatine; D003402:Creatine Kinase / Q000378:metabolism*; D005978:Glutathione; D008264:Macrophages / Q000201:enzymology*; D008626:Mercuribenzoates; D010710:Phosphates; D011650:Pulmonary Alveoli / Q000166:cytology; D011817:Rabbits; D013053:Spectrophotometry
chemical_list: D008626:Mercuribenzoates; D010710:Phosphates; D003402:Creatine Kinase; D005978:Glutathione; D003401:Creatine