A study of the subunit structure and the thiol reactivity of bovine liver uridine diphosphate glucose dehydrogenase.
1. The amino acid analysis of UDP-glucose dehydrogenase is reported. 2. N-Terminal-group analysis indicates only one type of N-terminal amino acid, methionine, to be present. 3. Peptide ;mapping' in conjunction with the amino acid analysis indicates that the subunits of the enzyme are similar if not identical. 4. The various kinetic classes of thiol group were investigated by reaction with 5,5'-dithiobis-(2-nitrobenzoate). 5. NAD(+), UDP-glucose and UDP-xylose protect the two rapidly reacting thiol groups of the hexameric enzyme. 6. Inactivation of the enzyme with 5,5'-dithiobis-(2-nitrobenzoate) indicates the involvement of six thiol groups in the maintenance of enzymic activity. 7. The pH-dependence of UDP-xylose inhibition of the enzyme was investigated. 8. The group involved in the binding of UDP-xylose to the protein has a heat of ionization of about 33kJ/mol and a pK of 8.4-8.6. 9. It is suggested that UDP-xylose has a cooperative homotropic effect on the enzyme.
D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001120:Arginine / Q000032:analysis; D001565:Benzoates; D002417:Cattle; D002854:Chromatography, Paper; D004586:Electrophoresis; D005947:Glucose; D008099:Liver / Q000201:enzymology*; D008715:Methionine / Q000032:analysis; D011487:Protein Conformation; D013057:Spectrum Analysis; D013439:Sulfhydryl Reagents; D013440:Sulfides; D014364:Tryptophan / Q000032:analysis; D014539:Uridine Diphosphate Sugars
D000596:Amino Acids; D001565:Benzoates; D013439:Sulfhydryl Reagents; D013440:Sulfides; D014539:Uridine Diphosphate Sugars; D014364:Tryptophan; D001120:Arginine; D008715:Methionine; D000429:Alcohol Oxidoreductases; D005947:Glucose