Preparation of metabolites of spironolactone by microbial oxygenation.
It has been postulated that 3-(3-oxo-7alpha-methylthio-4-androsten-17alpha-yl)propionic acid gamma-lactone (I) is a key intermediate in the metabolism of the anti-aldosterone agent, spironolactone (3-[3-oxo-7alpha-acetylthio-17beta-hydroxy-4-androsten-17alpha-yl] propionic acid gamma-lactone). In the present study it was found that microbial oxygenation of I by Chaetomium cochloides QM 624 gave three metabolites which retained sulfur in their molecules and were found to be identical to the human metabolites of spironolactone.
D001203:Ascomycota / Q000254:growth & development / Q000378:metabolism*; D001711:Biotransformation; D002855:Chromatography, Thin Layer; D003470:Culture Media; D005285:Fermentation; D006900:Hydroxylation; D013053:Spectrophotometry; D013148:Spironolactone / Q000378:metabolism*; D013454:Sulfoxides / Q000032:analysis*
D003470:Culture Media; D013454:Sulfoxides; D013148:Spironolactone