title: The persistence of hemopoietic stem cells in vitro.
abstract: Cells capable of forming colonies in spleens of irradiated mice (CFU) are lost temporarily when bone marrow cells from rats or mice are maintained in culture. Rat marrow CFU go through a minimum at about 3 days after which there is a slow increase in the number of CFU in culture, reaching a maximum at 9 days. Mouse marrow CFU reach a minimum at 3 days and a maximum at 7 days. Some rat marrow CFU persist in culture for as long as 28 days.
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D002999:Clone Cells; D046508:Culture Techniques; D006412:Hematopoietic Stem Cells; D007501:Iron / Q000378:metabolism; D007503:Iron Isotopes; D008297:Male; D051379:Mice; D013154:Spleen / Q000166:cytology; D013997:Time Factors
chemical_list: D007503:Iron Isotopes; D007501:Iron