Analysis of high-molecular-weight ribonucleic acid associated with intracisternal A particles.
Intracisternal A particles, known primarily for their association with various tumors, have been shown to contain high-molecular-weight (HMW) ribonucleic acid (RNA) by velocity centrifugation, using linear glycerol gradients. This HMW RNA is sensitive to ribonuclease digestion and alkali treatment but is resistant to Pronase treatment. By a double-labeling experiment, HMW RNA was shown to be intrinsic to intracisternal A particles and not to have resulted from cytoplasmic polysomal RNA aggregation. By a reconstitution experiment, it was determined that the results were not due to C-type virus contamination. The synthesis of HMW RNA in intracisternal A particles is inhibited by actinomycin D and ethidium bromide. These observations emphasize that there are probably some taxonomic relationships between intracisternal A particles and oncogenic RNA viruses.
D000818:Animals; D001854:Bone Marrow Cells; D002247:Carbon Isotopes; D002460:Cell Line; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003168:Complement Fixation Tests; D003609:Dactinomycin / Q000494:pharmacology; D004996:Ethidium / Q000494:pharmacology; D005260:Female; D005347:Fibroblasts; D007181:Inclusion Bodies, Viral; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D008970:Molecular Weight; D009368:Neoplasm Transplantation; D009447:Neuroblastoma; D009858:Oncogenic Viruses / Q000145:classification; D010954:Plasmacytoma; D012328:RNA Viruses / Q000145:classification; D012367:RNA, Viral / Q000032:analysis* / Q000096:biosynthesis; D014316:Tritium; D010948:Viral Plaque Assay
D002247:Carbon Isotopes; D012367:RNA, Viral; D014316:Tritium; D003609:Dactinomycin; D004996:Ethidium