Quantitative analysis of unconjugated and conjugated bile acids in duodenal fluid by densitometry after paper electrophoresis.
A new paper electrophoretic method for the separation of bile acids into five groups, (1) unconjugated, (2) glycine conjugates and (3) taurine conjugates, and (4) and (5) the respective monosulfates, is described. Rapid and accurate qualitative and quantitative estimations of each group are obtained by densitometry after internal standardization and phosphomolybdate color development. The technique can be done in the routine clinical laboratory and is useful for the detection of diseases affecting the enterohepatic circulation of bile acids.
D001647:Bile Acids and Salts / Q000032:analysis*; D003720:Densitometry; D004386:Duodenum / Q000378:metabolism*; D004590:Electrophoresis, Paper; D005767:Gastrointestinal Diseases / Q000378:metabolism; D006000:Glycocholic Acid / Q000032:analysis; D013464:Sulfuric Acids / Q000032:analysis; D013656:Taurocholic Acid / Q000032:analysis
D001647:Bile Acids and Salts; D013464:Sulfuric Acids; D013656:Taurocholic Acid; D006000:Glycocholic Acid