Cholesterol hydroperoxide formation in red cell membranes and photohemolysis in erythropoietic protoporphyria.
3beta-Hydroxy-5alpha-hydroperoxy-Delta(6)-cholestene is produced in protoporphyrin-containing red blood cell ghosts irradiated with approximately 400-nanometer light in the presence of oxygen. Incorporation of this cholesterol photooxidation product into normal red blood cells leads to increased osmotic fragility and eventual hemolysis. These results may be relevant to photohemolysis associated with erythropoietic protoporphyria.
D002247:Carbon Isotopes; D002462:Cell Membrane / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism / Q000494:pharmacology; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism*; D006461:Hemolysis; D006801:Humans; D009996:Osmotic Fragility / Q000187:drug effects; D010545:Peroxides / Q000096:biosynthesis* / Q000378:metabolism / Q000494:pharmacology; D010777:Photochemistry; D010782:Photolysis; D011164:Porphyrias / Q000097:blood* / Q000378:metabolism
D002247:Carbon Isotopes; D010545:Peroxides; D002784:Cholesterol