title: Freeze-fractured Acholeplasma laidlawii membranes: nature of particles observed.
abstract: Freeze-fracture of Acholeplasma laidlawii membranes from cells incubated in the presence of puromycin or omission of amino acids reveals a decrease in the number of particles between 50 and 100 angstroms in the hydrophobic fracture plane, which strongly suggests that these particles are protein. Additional evidence indicates that they may be involved in substrate transport.
mesh_terms: D000128:Acholeplasma laidlawii / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis; D002247:Carbon Isotopes; D002462:Cell Membrane / Q000032:analysis; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D005613:Freeze Etching; D005944:Glucosamine / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis; D008854:Microscopy, Electron; D009174:Mycoplasma / Q000166:cytology*; D010169:Palmitic Acids / Q000378:metabolism; D014176:Protein Biosynthesis; D011691:Puromycin / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014529:Uridine / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002247:Carbon Isotopes; D008055:Lipids; D010169:Palmitic Acids; D005973:Glutamine; D014316:Tritium; D011691:Puromycin; D005947:Glucose; D005944:Glucosamine; D013936:Thymidine; D014529:Uridine