Amelogenins. Purification and partial characterization of proteins from developing bovine dental enamel.
1. Procedures are described for the purification of amelogenin electrophoretic components and their analysis for homogeneity by polyacrylamide-gel electrophoresis at both acidic and alkaline pH values. 2. Most of these components belonged to two main groups, termed the J group and the C group after their major electrophoretic components. Sodium dodecyl sulphate-polyacrylamide-gel electrophoresis indicated that, within each group, proteins were of similar size, but the C-group proteins were larger than those of the J group. 3. By sedimentation-equilibrium ultracentrifugation and amino acid analysis, the four J-group components were found to be very small proteins (mol. wt. 5500-3000) and, except for one, similar in amino acid composition. The components of the C group were found to be proteins of moderate size (mol. wt. 16800-16100) with very similar amino acid compositions. A third minor amelogenin group of intermediate size was also found, but not further analysed. Details of the results of the ultracentrifuge studies are given in a supplementary paper that has been deposited as Supplementary Publication SUP 50014 at the National Lending Library for Science and Technology, Boston Spa, Yorks. LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1973) 131, 5. 4. Two of the J-group components were similar to amelogenins isolated by other workers. 5. All amelogenins analysed were rich in proline, glutamic acid, histidine and methionine, and contained no half-cystine. Their amino acid compositions, combined with their molecular weights, serve to distinguish the amelogenins from both collagens and keratins.
D000566:Amelogenesis; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003094:Collagen / Q000032:analysis; D003545:Cysteine / Q000032:analysis; D003743:Dental Enamel / Q000032:analysis* / Q000254:growth & development; D003746:Dental Enamel Proteins / Q000032:analysis / Q000302:isolation & purification*; D003956:Dialysis; D004591:Electrophoresis, Polyacrylamide Gel; D005971:Glutamates / Q000032:analysis; D006639:Histidine / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007633:Keratins / Q000032:analysis; D008715:Methionine / Q000032:analysis; D008963:Molar / Q000032:analysis; D008970:Molecular Weight; D011392:Proline / Q000032:analysis; D012967:Sodium Dodecyl Sulfate; D014461:Ultracentrifugation
D000596:Amino Acids; D003746:Dental Enamel Proteins; D005971:Glutamates; D012967:Sodium Dodecyl Sulfate; D006639:Histidine; D007633:Keratins; D003094:Collagen; D011392:Proline; D008715:Methionine; D003545:Cysteine