Mevalonate kinase in green leaves and etiolated cotyledons of the french bean Phaseolus vulgaris.
1. Partially purified preparations of mevalonate kinase were obtained from green leaves and etiolated cotyledons of Phaseolus vulgaris. 2. After removal of interfering polyphenols both enzyme preparations behaved identically on gel filtration, ion-exchange chromatography and density-gradient centrifugation. 3. The kinetic parameters of the preparations from the two sources were indistinguishable. The preparation from etiolated cotyledons had a K(m) of 4.26x10(-5)m for RS-mevalonate and 1.54x10(-3)m for ATP. The preparation from green leaves had a K(m) of 4.55x10(-5)m for RS-mevalonate and 1.75x10(-3)m for ATP. The pH optimum of both enzyme preparations was pH7.0. 4. The effect of inhibitors on the two enzyme preparations was similar, both being inhibited by reagents known to react with thiol groups, and the two preparations had similar inhibitor constants for competitive inhibition by prenyl pyrophosphates. 5. The molecular weight of the enzyme in both preparations was estimated to be 100000; the enzymes from the two preparations had similar mobilities on polyacrylamide-gel electrophoresis.
D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D002736:Chloroplasts / Q000201:enzymology; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002854:Chromatography, Paper; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D008798:Mevalonic Acid; D008970:Molecular Weight; D010636:Phenols / Q000494:pharmacology; D010770:Phosphotransferases / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D063245:Plant Development; D010944:Plants / Q000187:drug effects / Q000201:enzymology*; D013056:Spectrophotometry, Ultraviolet; D013439:Sulfhydryl Reagents; D013997:Time Factors
D002247:Carbon Isotopes; D010636:Phenols; D013439:Sulfhydryl Reagents; D008345:Manganese; D010770:Phosphotransferases; D008274:Magnesium; D008798:Mevalonic Acid