Gas chromatographic assay of epoxide hydrase activity with 3-methylcholanthrene-11,12-oxide.
Epoxide hydrase has been measured in rat tissue with 3-methylcholanthrene-11,12-oxide as substrate; diol formation was assayed by gas chromatographic separation of the trimethylsilylated derivative of trans-11,12-dlhydro-11,- 12-dihydroxy-3-methylcholanthrene from the corresponding derivative of the 11 (or 12)-hydroxy-3-methylcholanthrene on 3 percent OV-17, which is formed from the 11,12-oxide during the derivatization. The polycyclic hydrocarbons were extracted initially from the incubation mixture with ethyl acetate. The assay is simple, inexpensive, and sensitive.
D000818:Animals; D002849:Chromatography, Gas; D004988:Ethers, Cyclic; D006836:Hydro-Lyases / Q000378:metabolism*; D007421:Intestine, Small / Q000166:cytology / Q000201:enzymology; D007668:Kidney / Q000166:cytology / Q000201:enzymology; D008168:Lung / Q000166:cytology / Q000201:enzymology; D008297:Male; D008722:Methods; D008748:Methylcholanthrene / Q000378:metabolism*; D008861:Microsomes / Q000201:enzymology; D008862:Microsomes, Liver / Q000201:enzymology; D010087:Oxides; D051381:Rats; D013154:Spleen / Q000166:cytology / Q000201:enzymology
D004988:Ethers, Cyclic; D010087:Oxides; D008748:Methylcholanthrene; D006836:Hydro-Lyases