Studies on the developmental cycle of Chlamydia trachomatis: selective inhibition by hydroxyurea.
Hydroxyurea, a potent inhibitor of deoxyribonucleic acid synthesis, inhibits the development of trachoma agent when applied at a concentration of 5 x 10(-2) M. At a lower concentration, 5 x 10(-4) M, hydroxyurea permits the development of the trachoma inclusion bodies and initial bodies, but arrests the formation of elementary bodies, the infectious entity of the agent. The inhibitory effect of 5 x 10(-4) M hydroxyurea is reversible and can be used to synchronize the development of the agent. The synthesis of deoxyribonucleic acid, ribonucleic acid, and proteins takes place in the initial bodies after the removal of the inhibitor.
D000596:Amino Acids / Q000378:metabolism; D000650:Amnion; D001426:Bacterial Proteins / Q000096:biosynthesis; D002247:Carbon Isotopes; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002689:Chlamydia / Q000032:analysis / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D003562:Cytidine / Q000378:metabolism; D004269:DNA, Bacterial / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D006918:Hydroxyurea / Q000494:pharmacology*; D012329:RNA, Bacterial / Q000032:analysis / Q000096:biosynthesis; D013997:Time Factors; D014316:Tritium
D000596:Amino Acids; D001426:Bacterial Proteins; D002247:Carbon Isotopes; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D014316:Tritium; D003562:Cytidine; D006918:Hydroxyurea