Effect of polycations, polyanions and neuraminidase on the infectivity of trachoma-inclusin conjunctivitis and lymphogranuloma venereum organisms HeLa cells: sialic acid residues as possible receptors for trachoma-inclusion conjunction.
The infectivity of trachoma-inclusion conjunctivitis (TRIC) organisms (TW-5) was enhanced by pretreatment of HeLa cell monolayers before inoculation with diethylaminoethyl (DEAE)-dextran (30 mug/ml) and poly-l-lysine (10 mug/ml) and inhibited by dextran sulphate (250 mug/ml), fetuin (4%), ovomucoid (5%), N-acetyl neuraminic acid (0.5%), and Cholera vibrio neuraminidase (100 U/ml). The infectivity of lymphogranuloma venereum organisms (434) was not affected by DEAE-dextran, fetuin, and neuraminidase, was slightly inhibited by poly-l-lysine, and was inhibited by dextran-sulphate, ovomucoid, and N-acetyl neuraminic acid. The study suggested that sialic acid residues on the cell surface may be specific receptors for TRIC organisms. The receptors for TRIC organisms (TW-5 and TW-3) could be specifically blocked with inactivated (56 C for 30 min) TRIC organisms at the ratio of one live to 100 inactivated TRIC organisms, but not by inactivated lymphogranuloma venereum (434) or influenza virus (A(2)/Jap 305).
D001665:Binding Sites; D002413:Cations, Divalent / Q000494:pharmacology*; D002689:Chlamydia / Q000187:drug effects / Q000472:pathogenicity*; D003235:Conjunctivitis, Inclusion / Q000382:microbiology*; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D006367:HeLa Cells / Q000187:drug effects*; D006801:Humans; D007475:Ion Exchange Resins / Q000494:pharmacology*; D008219:Lymphogranuloma Venereum / Q000382:microbiology; D009438:Neuraminic Acids; D009439:Neuraminidase / Q000494:pharmacology*; D014141:Trachoma / Q000382:microbiology; D014774:Virulence
D002413:Cations, Divalent; D007475:Ion Exchange Resins; D009438:Neuraminic Acids; D009439:Neuraminidase