Synthesis of RNA-polyadenylic acid by isolated brain nuclei.
Nuclei, isolated from mouse brain tissue at various stages of postnatal development and incubated under cell-free conditions, synthesized RNA molecules that were associated with polyadenylic acid [poly(A)]. The RNA synthesized by these nuclei was similar to the poly(A)-associated products described for intact eukaryotic cells. The brain nuclei synthesized a similar proportion of RNA-poly(A) in the presence either of Mg(2+) or of Mn(2+) with (NH(4))(2)So(4). The RNA from neonatal brain nuclei appeared to have a greater proportion of poly(A)-containing RNA than nuclear products obtained from more mature neural tissue.
D000227:Adenine Nucleotides / Q000096:biosynthesis; D000367:Age Factors; D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D002474:Cell-Free System; D002482:Cellulose; D002846:Chromatography, Affinity; D006160:Guanosine Triphosphate / Q000378:metabolism; D066298:In Vitro Techniques; D008274:Magnesium; D008345:Manganese; D051379:Mice; D011119:Polynucleotides / Q000096:biosynthesis*; D012313:RNA / Q000096:biosynthesis* / Q000302:isolation & purification; D014316:Tritium
D000227:Adenine Nucleotides; D011119:Polynucleotides; D014316:Tritium; D008345:Manganese; D012313:RNA; D006160:Guanosine Triphosphate; D002482:Cellulose; D008274:Magnesium