Palmitoyl-coenzyme A synthetase. Mechanism of reaction.
The mechanism of long-chain fatty acid activation catalysed by highly purified microsomal palmitoyl-CoA synthetase was investigated. The kinetics of the overall reaction were found to conform to the Bi Uni Uni Bi Ping Pong mechanism. (18)O was transferred from [(18)O]palmitate to AMP and palmitoyl-CoA exclusively. The enzyme intermediate formed appeared to consist of enzyme-bound palmitate; this formation occurred only in the presence of ATP. However, the involvement of palmitoyl-AMP in the reaction catalysed by the purified enzyme has proved difficult to establish.
D000255:Adenosine Triphosphate; D001665:Binding Sites; D002247:Carbon Isotopes; D055598:Chemical Phenomena; D002621:Chemistry; D003066:Coenzyme A Ligases / Q000378:metabolism*; D005978:Glutathione / Q000494:pharmacology; D007553:Isotope Labeling; D007700:Kinetics; D008954:Models, Biological; D010100:Oxygen; D010103:Oxygen Isotopes; D010169:Palmitic Acids; D010759:Phosphorus Isotopes; D011485:Protein Binding; D013997:Time Factors; D014316:Tritium
D002247:Carbon Isotopes; D010103:Oxygen Isotopes; D010169:Palmitic Acids; D010759:Phosphorus Isotopes; D014316:Tritium; D000255:Adenosine Triphosphate; D003066:Coenzyme A Ligases; D005978:Glutathione; D010100:Oxygen