Ribonucleic acid polymerase in Allomyces arbuscula.
Three distinct species of ribonucleic acid (RNA) polymerase were resolved from Allomyces arbuscula by diethylaminoethyl-cellulose chromatography and characterized as to ionic strength and divalent cation preference. alpha-Amanitin specifically inhibited enzyme II; neither rifampin nor cycloheximide had any effect on the three enzymes. RNA polymerase was isolated from three stages of the diploid life cycle: the hyphal growth stage, mycelia in the process of forming sporangia, and the mitospores. The same three enzyme species could be resolved from each stage. Thus, there is no evidence from this work that RNA polymerase plays a major role in the control of development.
D002467:Cell Nucleus / Q000201:enzymology; D002474:Cell-Free System; D002848:Chromatography, DEAE-Cellulose; D003513:Cycloheximide / Q000494:pharmacology; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D005658:Fungi / Q000201:enzymology* / Q000254:growth & development; D009097:Multienzyme Complexes; D009183:Mycotoxins / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis; D012293:Rifampin / Q000494:pharmacology; D012995:Solubility; D013172:Spores, Fungal / Q000201:enzymology / Q000254:growth & development; D014316:Tritium; D014500:Uracil Nucleotides / Q000378:metabolism
D009097:Multienzyme Complexes; D009183:Mycotoxins; D014500:Uracil Nucleotides; D014316:Tritium; D003609:Dactinomycin; D012313:RNA; D003513:Cycloheximide; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin