Transmissible plasmid coding early enzymes of naphthalene oxidation in Pseudomonas putida.
The capacity of Pseudomonas putida PpG7 (ATCC 17,485) to grow on naphthalene, phenotype Nah(+), is lost spontaneously, and the frequency is increased by treatment with mitomycin C. The Nah(+) growth character can be transferred to cured or heterologous fluorescent pseudomonads lacking this capacity by conjugation, or between phage pf16-sensitive strains by transduction. After mutagenesis, strains can be selected with increased donor capacity in conjugation. Clones which use naphthalene grow on salicylate and carry catechol 2,3-oxygenase, the initial enzyme of the aromatic alpha-keto acid pathway, whereas cured strains grow neither on salicylate nor naphthalene and lack catechol 2,3-oxygenase, but retain catechol 1,2-oxygenase and the aromatic beta-keto adipate pathway enzymes.
D001435:Bacteriophages; D002396:Catechols; D004790:Enzyme Induction; D005827:Genetics, Microbial; D005838:Genotype; D008937:Mitomycins / Q000494:pharmacology; D009154:Mutation; D009281:Naphthalenes / Q000378:metabolism*; D009604:Nitrosoguanidines / Q000494:pharmacology; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000378:metabolism; D010641:Phenotype; D011549:Pseudomonas / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D014161:Transduction, Genetic
D002396:Catechols; D008937:Mitomycins; D009281:Naphthalenes; D009604:Nitrosoguanidines; D010105:Oxygenases