title: Benzoate metabolism in Pseudomonas putida(arvilla) mt-2: demonstration of two benzoate pathways.
abstract: Benzoate-grown cells of Pseudomonas putida(arvilla) mt-2 contain both metapyrocatechase and pyrocatechase activities, although the former activity is much higher than that of the latter. A spontaneous mutant deficient in metapyrocatechase and 2-hydroxymuconic semialdehyde hydrolyase, the first two enzymes in the meta-cleavage pathway of the ring of catechol, has been isolated from this strain. This mutant grows well on a minimal medium containing benzoate as a sole carbon source and has the high activity of pyrocatechase. These findings indicate that the strain mt-2 possesses the genetic capacity for enzymes of both the meta- and ortho-cleavage pathways of benzoate degradation, but its phenotypic expression is the meta pathway.
mesh_terms: D001565:Benzoates / Q000378:metabolism*; D002396:Catechols; D002474:Cell-Free System; D006836:Hydro-Lyases / Q000096:biosynthesis / Q000378:metabolism; D006861:Hydrogen Peroxide; D009153:Mutagens; D009154:Mutation; D009604:Nitrosoguanidines; D010105:Oxygenases / Q000096:biosynthesis / Q000378:metabolism; D011549:Pseudomonas / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D013053:Spectrophotometry
chemical_list: D001565:Benzoates; D002396:Catechols; D009153:Mutagens; D009604:Nitrosoguanidines; D006861:Hydrogen Peroxide; D010105:Oxygenases; D006836:Hydro-Lyases