Effects of glycerol on human adipose tissue triglyceride lipase activity.
Glycerol fully protects the human adipose tissue triglyceride lipase against the denaturing effects of high and low temperatures. Under such protection, storage of crude preparations at -10 degrees C or incubation at 50 degrees C resulted in a 1.5-3-fold increase of the measured lipase activity. This increase was shown to be related to enzyme newly released from tissular microparticles present in the samples. Advantage may be taken of these observations to improve greatly the conditions of extraction and storage of this lipase activity.
D000273:Adipose Tissue / Q000187:drug effects / Q000201:enzymology*; D002247:Carbon Isotopes; D002845:Chromatography; D002855:Chromatography, Thin Layer; D003080:Cold Temperature; D004355:Drug Stability; D005990:Glycerol / Q000494:pharmacology*; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D008722:Methods; D011489:Protein Denaturation; D013997:Time Factors; D014280:Triglycerides; D014304:Triolein / Q000378:metabolism; D014316:Tritium
D002247:Carbon Isotopes; D014280:Triglycerides; D014316:Tritium; D014304:Triolein; D008049:Lipase; D005990:Glycerol