Wheat-germ aspartate transcarbamoylase. The effects of ligands on the inactivation of the enzyme by trypsin and denaturing agents.
In the absence of added ligands aspartate transcarbamoylase (EC 2.1.3.2) from wheat germ is inactivated fairly rapidly by trypsin, by heat (60 degrees C), by highly alkaline conditions (pH11.3) and by sodium dodecyl sulphate. Addition of UMP alone, at low concentrations, decreases the rate of inactivation by each of these agents significantly. Carbamoyl phosphate alone does not alter the rate of inactivation by trypsin and by the detergent, but it antagonizes the effect of UMP in protecting the enzyme against these agents. These results have been interpreted to mean that two conformational states are reversibly accessible to the enzyme, namely an easily inactivated state favoured in the presence of carbamoyl phosphate and a more resistant state favoured in the presence of UMP. In the absence of ligands the enzyme is in the easily inactivated conformation. At very high concentrations l-aspartate also protects the enzyme but to a smaller extent than UMP. Some implications of these results are discussed.
D001221:Aspartate Carbamoyltransferase / Q000378:metabolism*; D001224:Aspartic Acid / Q000494:pharmacology; D002219:Carbamates; D002850:Chromatography, Gel; D003998:Dicarboxylic Acids / Q000494:pharmacology; D004355:Drug Stability; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008024:Ligands; D009943:Organophosphorus Compounds; D010944:Plants / Q000187:drug effects / Q000201:enzymology*; D011487:Protein Conformation; D011489:Protein Denaturation; D012967:Sodium Dodecyl Sulfate; D013997:Time Factors; D014908:Triticum; D014357:Trypsin / Q000378:metabolism / Q000494:pharmacology*; D014361:Trypsin Inhibitors / Q000494:pharmacology; D014500:Uracil Nucleotides / Q000494:pharmacology
D002219:Carbamates; D003998:Dicarboxylic Acids; D008024:Ligands; D009943:Organophosphorus Compounds; D014361:Trypsin Inhibitors; D014500:Uracil Nucleotides; D001224:Aspartic Acid; D012967:Sodium Dodecyl Sulfate; D001221:Aspartate Carbamoyltransferase; D014357:Trypsin