Turbidimetric assay of staphylococcal nuclease.
A simplified turbidimetric procedure was developed to assay staphylococcal nuclease activity. The ease of performance and sensitivity to nanogram quantities enhance the utilization of the method for the quantitative or qualitative estimation of the enzyme. Unlike plating methods, the turbidimetric procedure affords the differentiation between heat-stable and heat-labile nuclease activity.
D000818:Animals; D001881:Borates; D002021:Buffers; D002474:Cell-Free System; D003461:Crystallography; D004247:DNA / Q000378:metabolism; D003720:Densitometry; D003851:Deoxyribonucleases / Q000032:analysis*; D005069:Evaluation Studies as Topic; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008297:Male; D008722:Methods; D008836:Micrococcal Nuclease / Q000378:metabolism; D009691:Nucleic Acid Denaturation; D012474:Salmon; D013053:Spectrophotometry; D013094:Spermatozoa; D013210:Staphylococcus / Q000201:enzymology* / Q000254:growth & development; D013997:Time Factors
D001881:Borates; D002021:Buffers; D004247:DNA; D003851:Deoxyribonucleases; D008836:Micrococcal Nuclease