title: Biosynthesis of a mycobacterial lipopolysaccharide. Incorporation of (14C)acyl groups by whole cells in vivo.
abstract: 1. Mycobacterium phlei (A.T.C.C. 356) cells were incubated with (14)C-labelled short-chain fatty acids and the 6-O-methylglucose-containing lipopolysaccharides that became esterified with radioactive acyl groups were isolated. The pattern of labelling of these lipopolysaccharides with the different acyl groups, the effects of different conditions on labelling patterns, and the kinetics of the turnover of (14)C-labelled acyl groups were studied. 2. The labelling patterns are summarized as follows. [1-(14)C]Acetate was incorporated into all of the acyl groups. [1-(14)C]Propionate led to labelling of propionate and succinate, while [1-(14)C]isobutyrate was incorporated mostly as such, along with a trace amount in iso-octanoate. 3. Under the conditions of the experiments, [1-(14)C]acetate was rapidly incorporated into succinyl (3-carboxypropionyl) and octanoyl groups, whereas the acetyl groups themselves were labelled more slowly. Radioactivity in propionyl and succinyl groups, originating from [1-(14)C]propionate, attained maximum values and then gradually decreased in both. Incorporation of [1-(14)C]isobutyrate proceeded slowly but reached a plateau and remained constant. While n-butyrate is not a normal constituent of methyl-glucose-containing lipopolysaccharides, it was incorporated as such when n-[1-(14)C]-butyrate was supplied in the medium. 4. [1-(14)C]Acetyl groups were readily displaced by unlabelled acetate. On the other hand, the specific radioactivity of the succinyl group continued to increase during a 3h incubation with unlabelled succinate. Propionyl and succinyl groups, labelled by [1-(14)C]propionate, were displaced slowly by unlabelled propionate or succcinate. The isobutyryl group of the lipopolysaccharides did not turn over, in contrast to the results obtained with the other acyl substituents.
mesh_terms: D000085:Acetates / Q000378:metabolism; D002087:Butyrates / Q000378:metabolism; D002247:Carbon Isotopes; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D007553:Isotope Labeling; D007700:Kinetics; D008070:Lipopolysaccharides / Q000302:isolation & purification / Q000378:metabolism*; D008738:Methyl Ethers; D008954:Models, Biological; D008968:Molecular Conformation; D009161:Mycobacterium / Q000378:metabolism*; D011422:Propionates / Q000378:metabolism; D013329:Structure-Activity Relationship; D013386:Succinates / Q000378:metabolism; D013997:Time Factors
chemical_list: D000085:Acetates; D002087:Butyrates; D002247:Carbon Isotopes; D005230:Fatty Acids, Nonesterified; D008070:Lipopolysaccharides; D008738:Methyl Ethers; D011422:Propionates; D013386:Succinates