A method for the rapid qualitative and quantitative analysis of 4,4-dimethyl sterols.
A simple and relatively rapid technique has been developed for the separation of several 4,4-dimethyl steryl acetates, some of which contain sterically hindered nuclear double bonds. The method involves thin-layer chromatography on silver nitrate-impregnated silica gel and silver nitrate-impregnated alumina. The separated steryl acetates may then be analyzed quantitatively by gas-liquid chromatography.
D000085:Acetates / Q000032:analysis; D002247:Carbon Isotopes; D002776:Cholestanes / Q000032:analysis; D002782:Cholestenes / Q000032:analysis; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D007810:Lanosterol / Q000032:analysis; D008722:Methods; D008970:Molecular Weight; D012835:Silver Nitrate; D013261:Sterols / Q000032:analysis*
D000085:Acetates; D002247:Carbon Isotopes; D002776:Cholestanes; D002782:Cholestenes; D013261:Sterols; D007810:Lanosterol; D012835:Silver Nitrate