Occurrence of branched-cahin hydroxy fatty acids in Pseudomonas maltophilia.
Three branched-chain hydroxy acids not previously reported in other bacteria were found in extracts from saponified whole cells of Pseudomonas maltophilia. On the basis of evidence from mass spectrometry, infrared spectroscopy, and gas chromatographic procedures, they were identified as 2-hydroxy-9-methyldecanoic acid, 3-hydroxy-9-methyldecanoic acid, and 3-hydroxy-11-methyldodecanoic acid. These acids appeared to be tightly bound to other cellular components since they were not extracted from lyophilized cells with a chloroform-methanol (3:1) mixture.
D001966:Bromine; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D003470:Culture Media; D005227:Fatty Acids / Q000032:analysis* / Q000302:isolation & purification; D005463:Fluoroacetates; D006865:Hydrogenation; D013058:Mass Spectrometry; D011549:Pseudomonas / Q000032:analysis; D013055:Spectrophotometry, Infrared; D013997:Time Factors
D003470:Culture Media; D005227:Fatty Acids; D005463:Fluoroacetates; D001966:Bromine