title: Edman degradation on in vitro biosynthesized peptidoglycans from Staphylococcus epidermidis.
abstract: Edman degradations were performed on the pentapeptide bridges of peptidoglycans which were biosynthesized in vitro by using six different species of seryl-transfer ribonucleic acid (tRNA). The ratio of glycine to serine in each of the five bridge positions varied with the concentration of crude glycyl-tRNA contained in the reaction mixtures, and serine appeared to be incorporated in a random manner. No single species of seryl-tRNA could be identified as the one active in in vivo pentapeptide bridge synthesis; however, it can be speculated that seryl-tRNA(s) from category I could be involved in the in vivo nonrandom incorporation of serine in the bridge.
mesh_terms: D000595:Amino Acid Sequence; D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification; D002247:Carbon Isotopes; D002473:Cell Wall / Q000032:analysis; D002474:Cell-Free System; D002854:Chromatography, Paper; D003500:Cyclization; D005998:Glycine / Q000378:metabolism; D008722:Methods; D010457:Peptidoglycan / Q000032:analysis* / Q000096:biosynthesis; D012329:RNA, Bacterial / Q000302:isolation & purification / Q000378:metabolism; D012343:RNA, Transfer / Q000302:isolation & purification / Q000378:metabolism; D012694:Serine / Q000378:metabolism; D013210:Staphylococcus / Q000032:analysis* / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D014316:Tritium
chemical_list: D002247:Carbon Isotopes; D010457:Peptidoglycan; D012329:RNA, Bacterial; D014316:Tritium; D012694:Serine; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases; D005998:Glycine