The N-acetylhexosaminidase components of the ram testis and epididymis.
Three and four N-acetylhexosaminidase components, from ram testis and epididymis respectively, have been separated by ion-exchange chromatography on DEAE-cellulose. Although they all have the same molecular weight (approx. 140000) and very similar catalytic properties towards the synthetic substrates, 4-methylumbelliferyl N-acetyl-beta-glucosaminide and N-acetyl-beta-galactosaminide, isoelectric focusing of the individual components showed that each had a distinct pI value. Isoelectric focusing has also been used to demonstrate the occurrence of multiple forms in ejaculated ram semen.
D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004822:Epididymis / Q000201:enzymology*; D006596:Hexosaminidases / Q000302:isolation & purification*; D007525:Isoelectric Focusing; D008297:Male; D008722:Methods; D008970:Molecular Weight; D012661:Semen / Q000201:enzymology; D012756:Sheep; D013737:Testis / Q000201:enzymology*
D006596:Hexosaminidases