Temperature variation of polyribonucleotide conformation by an interaction with basic globular proteins.
Chicken lysozyme interacts with polyribonucleotides to form large light-scattering centers. The size of these is critically dependent on conformation of the polynucleotides. Hence, the interaction provides a very sensitive method of determining temperature intervals within which the polynucleotides change from multistranded, stacked-base structures to single-stranded, random coils. This change can be observed with the unaided eye, especially when concentrations of polynucleotides are of the order of a few micrograms per milliliter.
D000227:Adenine Nucleotides; D000818:Animals; D002645:Chickens; D003597:Cytosine Nucleotides; D006150:Guanine Nucleotides; D007292:Inosine Nucleotides; D009113:Muramidase; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D010179:Pancreas / Q000201:enzymology; D011072:Poly U; D011119:Polynucleotides; D012343:RNA, Transfer; D012260:Ribonucleases; D012265:Ribonucleotides; D012542:Scattering, Radiation; D013696:Temperature; D015003:Yeasts
D000227:Adenine Nucleotides; D003597:Cytosine Nucleotides; D006150:Guanine Nucleotides; D007292:Inosine Nucleotides; D011119:Polynucleotides; D012265:Ribonucleotides; D011072:Poly U; D012343:RNA, Transfer; D012260:Ribonucleases; D009113:Muramidase