Preparation of metabolically active Staphylococcus aureus protoplasts by use of the Aeromonas hydrophila lytic enzyme.
Stable, metabolically active protoplasts of Staphylococcus aureus have been prepared by the use of a staphylolytic enzyme produced by Aeromonas hydrophila. Respiratory and glycolytic rates and synthesis of nucleic acids, protein, and lipid in these protoplasts, stabilized variously in 1.1 M sucrose, 0.37 M sodium succinate, or 0.37 M sodium sulfate, have been shown to be comparable with the same parameters in intact cells under the same conditions.
D000085:Acetates / Q000378:metabolism; D000333:Aeromonas / Q000201:enzymology; D000596:Amino Acids / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis; D002247:Carbon Isotopes; D002473:Cell Wall / Q000378:metabolism; D004269:DNA, Bacterial / Q000096:biosynthesis; D004798:Enzymes / Q000378:metabolism*; D006019:Glycolysis; D008055:Lipids / Q000096:biosynthesis; D008854:Microscopy, Electron; D010101:Oxygen Consumption; D011523:Protoplasts / Q000166:cytology / Q000378:metabolism; D012329:RNA, Bacterial / Q000096:biosynthesis; D013210:Staphylococcus / Q000166:cytology / Q000302:isolation & purification* / Q000378:metabolism; D013386:Succinates / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014498:Uracil / Q000378:metabolism
D000085:Acetates; D000596:Amino Acids; D001426:Bacterial Proteins; D002247:Carbon Isotopes; D004269:DNA, Bacterial; D004798:Enzymes; D008055:Lipids; D012329:RNA, Bacterial; D013386:Succinates; D013431:Sulfates; D014316:Tritium; D014498:Uracil; D013395:Sucrose; D013936:Thymidine