The purification and properties of factor X from pig serum and its role in hypercoagulability in vivo.
The molecular weights or shapes of Factor X preparations determined by gel filtration were dependent on the density of the BaSO(4) used for the initial adsorption from serum. One form obtained with BaSO(4) of density 2g/ml behaved as if it had a molecular weight of 39000 and possessed preformed clotting activity (Factor Xa), whereas that of the form adsorbed with BaSO(4) of density 1g/ml had a molecular weight of 69000 and consisted of inactive Factor X precursor. Thus degradation accompanied by activation seems to occur as a result of surface adsorption on high-density BaSO(4) and is associated with an interchange of protein between the two bands observed electrophoretically. The clotting and esterase activities measurable in vitro after complete activation were not matched by a corresponding ability to induce thrombus formation and ;lethality' in vivo. The most effective preparations of Factor X in this respect possessed preformed activity, which was enhanced in the presence of phospholipid. Factor X lost activity more rapidly in dilute solution, and its concentration at the surface of phospholipid micelles probably decreases loss by dilution in circulating blood.
D000327:Adsorption; D000818:Animals; D001466:Barium Sulfate; D001777:Blood Coagulation; D001780:Blood Coagulation Tests; D002241:Carbohydrates / Q000032:analysis; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004355:Drug Stability; D004586:Electrophoresis; D005170:Factor X / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D006358:Hot Temperature; D007700:Kinetics; D008970:Molecular Weight; D010743:Phospholipids; D011134:Polysaccharides; D011489:Protein Denaturation; D012911:Snakes; D013056:Spectrophotometry, Ultraviolet; D013552:Swine; D013997:Time Factors; D014462:Ultrafiltration; D014688:Venoms / Q000378:metabolism
D002241:Carbohydrates; D010743:Phospholipids; D011134:Polysaccharides; D014688:Venoms; D001466:Barium Sulfate; D005170:Factor X