Simplification of urinary aldosterone measurement by radioimmunoassay.
An anti-aldosterone serum has been used to measure urinary aldosterone concentration. A single stage of paper chromatography provides an extract of sufficient purity. Further purification by additional chromatography in a different solvent system only improves the purity by 3% +/- 8 (SD). Blanks are negligible and recovery of added aldosterone is 104% +/- 10 (SD).A simplified rapid screening assay capable of revealing gross deviations from the normal is also suggested. The diagnostic value of the assay is indicated.
D000450:Aldosterone / Q000652:urine*; D002854:Chromatography, Paper; D006801:Humans; D008722:Methods; D011863:Radioimmunoassay; D014316:Tritium
D014316:Tritium; D000450:Aldosterone