Monoribosomal attachment to messenger ribonucleic acid in sodium fluoride-treated rabbit reticulocytes.
Mild proteolysis with Pronase selectively dissociates ribosomes not attached to mRNA into subunits; ribosomes attached to mRNA remain intact. A portion of monoribosomes from reticulocytes incubated with NaF resisted proteolytic dissociation. Recovery of mRNA from monoribosomes of NaF-treated reticulocytes therefore may be explained by persistent attachment of some monoribosomes to mRNA.
D000818:Animals; D001665:Binding Sites; D002499:Centrifugation, Density Gradient; D005459:Fluorides / Q000494:pharmacology*; D066298:In Vitro Techniques; D011402:Pronase; D011485:Protein Binding; D012333:RNA, Messenger / Q000097:blood* / Q000378:metabolism; D051381:Rats; D012156:Reticulocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*
D012333:RNA, Messenger; D011402:Pronase; D005459:Fluorides