Diisopropylfluorophosphate: suppression of ionic conductance of the cholinergic receptor.
When frog sartorius muscles were exposed to diisopropylfluorophosphate, the amplitude and half-decay time of the end-plate current decreased; the half-decay time became almost potential-independent and the equilibrium potential for the end-plate current was more negative than during control conditions. When the excess reagent was removed by washing so that only the phosphorylated acetylcholinesterase remained, the amplitude of the end-plate current was restored, while its half-decay time was markedly increased. These findings reveal that this organophosphate significantly affects the receptor-ionic conductance modulator complex in addition to its well-known anticholinesterase activity.
D000818:Animals; D001001:Anura; D001692:Biological Transport / Q000187:drug effects; D004558:Electric Stimulation; D007531:Isoflurophate / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D009119:Muscle Contraction; D009469:Neuromuscular Junction / Q000187:drug effects* / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D011950:Receptors, Cholinergic / Q000187:drug effects; D011955:Receptors, Drug / Q000187:drug effects*; D012964:Sodium / Q000378:metabolism
D011950:Receptors, Cholinergic; D011955:Receptors, Drug; D007531:Isoflurophate; D012964:Sodium; D011188:Potassium