title: Uric acid. Characterization of its interaction with human serum albumin.
abstract: Binding of sodium urate to human serum albumin (HSA) was measured by continuous ultrafiltration at pH 7.4 and ionic strength 0.16 over the concentration range 1-13 mg/100 ml. The percent sodium urate bound to 5 g/100 ml HSA was constant over this concentration range: 30.3 (SE+/-0.6)% being bound at 4 degrees C, 22.6+/-0.3% at 22.5 degrees C, and 19.6+/-0.3% at 37 degrees C. Derived association constants, assuming one binding site were 6.0 x 10(2) M(-1) (4 degrees C), 4.47 x 10(2) M(-1) (22.5 degrees C), and 3.88 x 10(2) M(-1) (37 degrees C).
mesh_terms: D001665:Binding Sites; D002021:Buffers; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008722:Methods; D011485:Protein Binding; D012709:Serum Albumin; D013696:Temperature; D014462:Ultrafiltration; D014527:Uric Acid
chemical_list: D002021:Buffers; D012709:Serum Albumin; D014527:Uric Acid