An automated passive haemagglutination technique suitable for the detection of hepatitis B virus antigen and antibody in blood donors.
A method is described for preparing purified hepatitis B antigen (HB Ag) viral antigen without density gradient centrifugation. A method for sensitizing human group O red cells with this preparation is given, together with the technical details of an automated passive haemagglutination technique suitable for the mass screening of blood donors for HB virus and anti-HB antibody.
D000327:Adsorption; D000914:Antibodies, Viral / Q000032:analysis*; D000941:Antigens; D001322:Autoanalysis; D001331:Automation; D001782:Blood Donors; D001789:Blood Group Antigens; D004912:Erythrocytes / Q000276:immunology; D006386:Hemagglutination Tests; D006511:Hepatitis B Antigens / Q000302:isolation & purification*; D006801:Humans; D007106:Immune Sera; D007122:Immunoelectrophoresis; D008403:Mass Screening; D008722:Methods
D000914:Antibodies, Viral; D000941:Antigens; D001789:Blood Group Antigens; D006511:Hepatitis B Antigens; D007106:Immune Sera