title: Promotion of replication in lymphoid cells by specific thiols and disulfides in vitro. Effects on mouse lymphoma cells in comparison with splenic lymphocytes.
abstract: Numerous lines of mouse lymphoid tumors (13 of 22 tested) showed, with increased sensitivity, a property of normal mouse splenic lymphocytes, the potential for growth promotion in vitro by specific thiols added to standard culture media. For lymphoma L1210 (V), structure activity relationships were examined; 9 of 30 thiols promoted growth; the most active was alpha-thioglycerol, effective at 0.2 microM. Thiols became oxidized under conditions of tissue culture and had half-lives of less than 8 h. Disulfides of active thiols promoted growth of lymphoma cells. The mitogenic response of splenic lymphocytes to lectins was increased by thiols-disulfides which promoted the growth of lymphoma cells, but the response varied with the mitogen preparation used and under some conditions thiols-disulfides were inhibitory.
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D003545:Cysteine / Q000494:pharmacology; D004220:Disulfides / Q000494:pharmacology*; D006207:Half-Life; D007939:Leukemia L1210; D008214:Lymphocytes / Q000187:drug effects*; D008223:Lymphoma; D008623:Mercaptoethanol / Q000494:pharmacology; D051379:Mice; D008934:Mitogens / Q000494:pharmacology*; D008938:Mitosis / Q000187:drug effects*; D013154:Spleen / Q000166:cytology; D013329:Structure-Activity Relationship; D013438:Sulfhydryl Compounds / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism
chemical_list: D004220:Disulfides; D008934:Mitogens; D013438:Sulfhydryl Compounds; D008623:Mercaptoethanol; D003545:Cysteine; D013936:Thymidine