Peroxidase-mediated mammalian cell cytotoxicity.
Lactoperoxidase, in the presence of hydrogen peroxide and iodide is cytotoxic for human and mouse lymphoid cells, and human erythrocytes. Myeloperoxidase, in amounts equivalent to 1.5 x 10(6) neutrophils, readily replaces lactoperoxidase, and allows the substitution of the iodide ion by chloride. The myeloperoxidase-mediated reaction is rapid, and highly efficient, leading to 85-90% cell death in 90 min, as measured by (51)chromium release and dye exclusion. The mixture of granulocytes. monocytes, and lymphocytes present in an inflammatory exudate, and the intimate cell-to-cell association characteristic of cytotoxic phenomena may provide the in vivo requirements for such a system.
D000818:Animals; D002478:Cells, Cultured; D002859:Chromium Isotopes; D003601:Cytotoxicity Tests, Immunologic; D004912:Erythrocytes / Q000187:drug effects / Q000276:immunology; D005949:Glucose Oxidase / Q000494:pharmacology; D007454:Iodides / Q000494:pharmacology; D007553:Isotope Labeling; D007939:Leukemia L1210; D007962:Leukocytes / Q000187:drug effects / Q000276:immunology; D008722:Methods; D051379:Mice; D010544:Peroxidases / Q000494:pharmacology*; D014343:Trypan Blue
D002859:Chromium Isotopes; D007454:Iodides; D005949:Glucose Oxidase; D010544:Peroxidases; D014343:Trypan Blue