Rapid solid-phase radioimmunoassay for staphylococcal enterotoxin A.
A rapid solid-phase radioimmunoassay for staphylococcal enterotoxin A is described. The assay procedure requires 3 to 4 h for completion by using a competitive inhibition system in which the antibody is attached to bromacetyl cellulose particles. It is accurate to a level of 0.01 mug of enterotoxin A/ml in a variety of media such as ham, milk products, crab meat, custard, etc. No significant interference was found with any media or food product tested.
D000907:Antibodies, Bacterial / Q000302:isolation & purification; D000936:Antigen-Antibody Complex; D002482:Cellulose; D004768:Enterotoxins / Q000032:analysis*; D005189:False Positive Reactions; D005516:Food Microbiology; D007074:Immunoglobulin G / Q000302:isolation & purification; D007456:Iodine Isotopes; D011863:Radioimmunoassay; D013210:Staphylococcus
D000907:Antibodies, Bacterial; D000936:Antigen-Antibody Complex; D004768:Enterotoxins; D007074:Immunoglobulin G; D007456:Iodine Isotopes; D002482:Cellulose