title: Interaction of bretylium and guanethidine on the relaxations of the rat isolated fundal strip preparation, evoked by indirect stimulation.
abstract: 1. Isolated rat stomach fundal strip bathed in Krebs solution containing atropine (1 mug/ml), responded to indirect stimulation by a relaxation which was frequency dependent. These responses were blocked by phenoxybenzamine (6 mug/ml) or phentolamine (8 mug/ml).2. Strips obtained from rats previously treated with reserpine did not show relaxation to indirect stimulation. These responses were therefore adrenergic in nature.3. Bretylium (0.1-100 mug/ml) failed to block the relaxations produced by indirect stimulation, in fact relaxations were potentiated by the drug.4. Guanethidine (10 mug/ml) blocked the relaxations induced by indirect stimulation.5. Guanethidine may be taken up by adrenergic nerves actively since its action is not seen at 12 degrees C.6. Bretylium (10 mug/ml) prevented the actions of guanethidine at 37 degrees C.
mesh_terms: D000818:Animals; D001949:Bretylium Compounds / Q000494:pharmacology*; D004347:Drug Interactions; D004558:Electric Stimulation; D006145:Guanethidine / Q000494:pharmacology*; D066298:In Vitro Techniques; D009638:Norepinephrine / Q000494:pharmacology; D010643:Phenoxybenzamine / Q000494:pharmacology; D010646:Phentolamine / Q000494:pharmacology; D051381:Rats; D013270:Stomach / Q000187:drug effects*
chemical_list: D001949:Bretylium Compounds; D010643:Phenoxybenzamine; D009638:Norepinephrine; D010646:Phentolamine; D006145:Guanethidine