title: Phospholipase A-1 of human blood platelets.
abstract: Substantial phospholipase A(1) activity has been demonstrated in human blood platelets, and a rapid method for its measurement is described. The enzyme requires taurocholate for full activity and in these conditions the pH optimum is 4.8. The phospholipase activity is released from platelets by incubation with thrombin.
mesh_terms: D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D002247:Carbon Isotopes; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008247:Lysosomes / Q000201:enzymology; D008722:Methods; D009994:Osmolar Concentration; D010713:Phosphatidylcholines; D010740:Phospholipases / Q000097:blood*; D012964:Sodium / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013656:Taurocholic Acid / Q000494:pharmacology; D013917:Thrombin / Q000494:pharmacology; D014316:Tritium
chemical_list: D002247:Carbon Isotopes; D010713:Phosphatidylcholines; D014316:Tritium; D013656:Taurocholic Acid; D012964:Sodium; D010740:Phospholipases; D013917:Thrombin