Characterization of nucleic acid of pichinde virus.
The nucleic acid of Pichinde virus was found to be single-stranded ribonucleic acid (RNA) as determined by sensitivity to ribonuclease, by alkaline degradation, by buoyant density in cesium sulfate, and by analysis of the base composition. The RNA of the virion could be separated into five components which had sedimentation coefficients corresponding to 31S, 28S, 22S, 18S and 4 to 6S. The 28S, 18S, and possibly the 4 to 6S RNAs appear to be derived from host cell components incorporated into the virion, whereas the 31S and 22S components appear to represent the genome of the virus.
D000818:Animals; D002247:Carbon Isotopes; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D003596:Cytosine / Q000032:analysis; D003609:Dactinomycin / Q000494:pharmacology; D004590:Electrophoresis, Paper; D004591:Electrophoresis, Polyacrylamide Gel; D006151:Guanosine / Q000032:analysis; D006367:HeLa Cells; D006801:Humans; D007668:Kidney; D008970:Molecular Weight; D010758:Phosphorus; D012328:RNA Viruses / Q000032:analysis* / Q000254:growth & development / Q000302:isolation & purification; D012335:RNA, Ribosomal / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D012260:Ribonucleases; D014316:Tritium
D002247:Carbon Isotopes; D012335:RNA, Ribosomal; D012367:RNA, Viral; D014316:Tritium; D006151:Guanosine; D003609:Dactinomycin; D010758:Phosphorus; D003596:Cytosine; D012260:Ribonucleases