Bacterial metabolism of 3-hydroxy-3-methylglutaric acid.
An organism belonging to Pseudomonadaceae and capable of utilizing 3-hydroxy-3-methylglutarate as sole carbon source has been isolated from soil. Whole-cell preparations catalyze the oxidation of acetoacetate, acetate, glyoxylate, and citric acid cycle intermediates. Cell-free extracts of 3-hydroxy-3-methylglutarate-grown cells show an adenosine triphosphate, coenzyme A (CoA), and Mg(2+)-dependent conversion of 3-hydroxy-3-methylglutarate to 3-hydroxy-3-methylglutaryl-CoA. Succinyl-CoA-generating system has no effect on the activation and catabolism of 3-hydroxy-3-methylglutarate.
D000085:Acetates / Q000378:metabolism; D000090:Acetoacetates / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000494:pharmacology; D001419:Bacteria / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D002245:Carbon Dioxide / Q000096:biosynthesis; D002474:Cell-Free System; D002854:Chromatography, Paper; D002951:Citrates / Q000378:metabolism; D003065:Coenzyme A / Q000494:pharmacology; D003470:Culture Media; D005977:Glutarates / Q000378:metabolism*; D006038:Glyoxylates / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D008365:Manometry; D008745:Methylation; D008798:Mevalonic Acid / Q000378:metabolism; D010084:Oxidation-Reduction; D007652:Oxo-Acid-Lyases / Q000378:metabolism; D010101:Oxygen Consumption; D012988:Soil Microbiology; D013386:Succinates / Q000378:metabolism; D013869:Thiolester Hydrolases / Q000378:metabolism
D000085:Acetates; D000090:Acetoacetates; D002951:Citrates; D003470:Culture Media; D005977:Glutarates; D006038:Glyoxylates; D013386:Succinates; D002245:Carbon Dioxide; D000255:Adenosine Triphosphate; D013869:Thiolester Hydrolases; D007652:Oxo-Acid-Lyases; D008274:Magnesium; D008798:Mevalonic Acid; D003065:Coenzyme A