title: The interaction of the polyene antibiotic lucensomycin with cholesterol in erythrocyte membranes and in model systems. II. Cooperative effects in erythrocyte membranes.
abstract: Fluorometric titration curves of erythrocyte membranes with increasing lucensomycin have a sigmoid shape. This behavior, which was not present when colloidal cholesterol suspensions were used, is, however, not peculiar to the membrane structure, being also present in cholesterol-containing phospholipid micelles. Addition of acetic acid induced or increased sigmoidicity. This behavior can either be due to a true cooperativity in binding or to different fluorescence yields of the various lucensomycin-membrane complexes. The latter hypothesis appears to be slightly favored.
mesh_terms: D000085:Acetates; D000818:Animals; D000935:Antifungal Agents / Q000378:metabolism; D001665:Binding Sites; D002417:Cattle; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D003102:Colloids; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006027:Glycosides / Q000378:metabolism; D046911:Macromolecular Substances; D008433:Mathematics; D010713:Phosphatidylcholines; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000085:Acetates; D000935:Antifungal Agents; D003102:Colloids; D006027:Glycosides; D046911:Macromolecular Substances; D010713:Phosphatidylcholines; D012967:Sodium Dodecyl Sulfate; D002784:Cholesterol