Diffusion of weak acids across lipid bilayer membranes: effects of chemical reactions in the unstirred layers.
Chemical reactions in the aqueous unstirred layers of solution adjacent to a membrane can have dramatic effects on the diffusion of solutes across that membrane. This is demonstrated by the diffusion of labeled salicylate and salicylic acid across a phospholipid bilayer membrane. Two types of chemical reactions are considered. The first is an isotopic exchange reaction between the ionic and nonionic forms of a weak acid, HA + *A(-) [unknown] H*A + A(-). This reaction provides a way of estimating the true membrane permeability to a highly permeant weak acid and also a way of estimating the thickness of the unstirred layers. The second chemical reaction, the dissociation of a weak acid, HA [unknown] H(+) + A(-), can be used to show how the presence or absence of buffers in the unstirred layers controls the net transport of permeant weak acids across a membrane. In principle, the addition of appropriate "antacid" buffers to salicylates can increase their rate of absorption from the stomach.
D002250:Carbon Radioisotopes; D055598:Chemical Phenomena; D002621:Chemistry; D004058:Diffusion; D006863:Hydrogen-Ion Concentration; D008564:Membrane Potentials; D008567:Membranes, Artificial; D008956:Models, Chemical; D010477:Perfusion; D010539:Permeability; D012459:Salicylates
D002250:Carbon Radioisotopes; D008567:Membranes, Artificial; D012459:Salicylates