Repressor and antirepressor in the regulation of staphylococcal penicillinase synthesis.
5-methyltryptophan (5MT) induces penicillinase synthesis in Staphylococcus aureus. The analog is incorporated into protein by both wild-type and tryptophan-starved cells. Since normal penicillinase repressor appears to contain tryptophan even though penicillinase itself does not, it is concluded that 5MT induces penicillinase synthesis by becoming incorporated into the penicillinase repressor and thereby inactivating the repressor. Thus biochemical data support the existence of a penicillinase repressor and indicate that penicillinase synthesis is regulated by negative control and not by positive control.-In the absence of exogenous tryptophan, staphylococcal penicillinase induction can be inhibited by 7-azatryptophan (7azaT). Because 7azaT is incorporated into protein by tryptophan-starved cells, it is concluded that 7azaT blocks penicillinase induction by inactivating a penicillinase regulatory protein into which the analog has been incorporated. Incorporation of 7azaT does not appear to inactivate the operator binding site or the effector binding site on the penicillinase repressor. Therefore, it appears that 7azaT blocks penicillinase induction by inactivating the penicillinase antirepressor, a protein required for inactivation of the penicillinase repressor and, hence, required for penicillinase induction.
D001372:Aza Compounds / Q000378:metabolism / Q000494:pharmacology; D002250:Carbon Radioisotopes; D003720:Densitometry; D004790:Enzyme Induction; D005696:Galactosidases / Q000096:biosynthesis; D005809:Genes, Regulator; D007700:Kinetics; D008954:Models, Biological; D009154:Mutation; D010405:Penicillinase / Q000096:biosynthesis*; D013210:Staphylococcus / Q000187:drug effects / Q000201:enzymology*; D013329:Structure-Activity Relationship; D013997:Time Factors; D014316:Tritium; D014364:Tryptophan / Q000378:metabolism / Q000494:pharmacology; D065093:beta-Lactamase Inhibitors
D001372:Aza Compounds; D002250:Carbon Radioisotopes; D065093:beta-Lactamase Inhibitors; D014316:Tritium; D014364:Tryptophan; D005696:Galactosidases; D010405:Penicillinase