Terminal sequence studies of high-molecular-weight ribonucleic acid. The 3' termini of rabbit globin messenger ribonucleic acid.
Haemoglobin mRNA isolated from EDTA-treated polyribosomes has an apparent molecular weight of 120000-180000 estimated by condensation with (3)H-labelled isoniazid after periodate oxidation. Analysis of the ribonuclease digests of isoniazid-labelled RNA by paper electrophoresis and column chromatography enables the amount of contaminating 18S, 7S, 5S and 4S RNA to be estimated, and a corrected molecular weight of globin mRNA as the acid is 161000 or 500 nucleotides in length. This molecule contains two groups of 3'-terminal sequences in equal yield; G-Y-A(6) and G-Y-A(7) in the ratio 3:2, and G-N(9-16)-Y-A(2) and G-N(9-16)-Y-N(3) in the ratio 3:2. The significance of these sequences is discussed in relation to the poly(A) content of globin mRNA, the specificity of the sequences, and possible function in processing and biosynthesis of mRNA.
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D004590:Electrophoresis, Paper; D004591:Electrophoresis, Polyacrylamide Gel; D005914:Globins; D007538:Isoniazid; D008970:Molecular Weight; D009841:Oligonucleotides / Q000032:analysis; D010179:Pancreas; D012333:RNA, Messenger / Q000032:analysis* / Q000302:isolation & purification; D012335:RNA, Ribosomal / Q000032:analysis* / Q000302:isolation & purification; D011817:Rabbits; D012156:Reticulocytes / Q000032:analysis* / Q000166:cytology; D012260:Ribonucleases; D012265:Ribonucleotides / Q000032:analysis; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet; D014316:Tritium
D002247:Carbon Isotopes; D009841:Oligonucleotides; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012265:Ribonucleotides; D014316:Tritium; D005914:Globins; D012260:Ribonucleases; D007538:Isoniazid