Metabolism of biphenyl. 2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoate: the meta-cleavage product from 2,3-dihydroxybiphenyl by Pseudomonas putida.
1. 2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid was isolated and identified from washed suspensions of Pseudomonas putida incubated in the presence of 2,3-dihydroxybiphenyl. 2. Benzoic acid was isolated from reaction mixtures of crude cell-free extracts incubated with 2,3-dihydroxybiphenyl. 3. The presence in the same reaction mixtures of either 4-hydroxy-2-oxovalerate or 2-hydroxypenta-2,4-dienoate was suggested by mass spectrometry. 4. The degradative pathway of biphenyl is discussed.
D001565:Benzoates / Q000302:isolation & purification; D001713:Biphenyl Compounds / Q000378:metabolism*; D002474:Cell-Free System; D002855:Chromatography, Thin Layer; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis*; D007651:Keto Acids; D013058:Mass Spectrometry; D011549:Pseudomonas / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D001565:Benzoates; D001713:Biphenyl Compounds; D005231:Fatty Acids, Unsaturated; D007651:Keto Acids; C018971:2-hydroxy-6-oxo-6-phenyl-2,4-hexadienoic acid