Protein synthesis in vitro in a system from plant mitochondria.
A mitochondrial system from 48h-germinating seeds of Vigna sinensis (Linn.) Savi is capable of incorporating l-[U-(14)C]valine into proteins and is practically insensitve to cycloheximide, but highly sensitive to chloramphenicol and fusidic acid, a potent inhibitor of peptide-chain elongation factor. A system consisting of mitochondrial S-100 fraction and ribosomes from the same source and other cofactors is capable of polyphenylalanine synthesis and behaves similarly with respect to these inhibitors.
D002250:Carbon Radioisotopes; D002701:Chloramphenicol / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D005672:Fusidic Acid / Q000494:pharmacology; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D010649:Phenylalanine / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis*; D012270:Ribosomes / Q000378:metabolism; D012639:Seeds / Q000378:metabolism*; D014633:Valine / Q000378:metabolism
D002250:Carbon Radioisotopes; D010940:Plant Proteins; D010649:Phenylalanine; D005672:Fusidic Acid; D002701:Chloramphenicol; D003513:Cycloheximide; D014633:Valine