2,4-Dichlorophenoxyacetate metabolism by Arthrobacter sp.: accumulation of a chlorobutenolide.
An enzyme preparation from 2,4-dichlorophenoxyacetate-grown Arthrobacter sp. converted cis,cis-2,4-dichloromuconate to chloromaleylacetate. The enzyme lactonizing the dichloromuconate to yield 2-chloro-4-carboxymethylene but-2-enolide was separated from the butenolide-delactonizing enzyme.
D015084:2,4-Dichlorophenoxyacetic Acid / Q000378:metabolism*; D000272:Adipates; D000645:Ammonium Sulfate; D001173:Arthrobacter / Q000201:enzymology / Q000378:metabolism*; D002458:Cell Fractionation; D002474:Cell-Free System; D002848:Chromatography, DEAE-Cellulose; D004798:Enzymes / Q000378:metabolism; D007535:Isomerases / Q000032:analysis; D007783:Lactones / Q000096:biosynthesis*; D013057:Spectrum Analysis
D000272:Adipates; D004798:Enzymes; D007783:Lactones; D015084:2,4-Dichlorophenoxyacetic Acid; D007535:Isomerases; D000645:Ammonium Sulfate