Ribonucleic acid synthesis by spermatozoa from the rat and hamster.
Spermatozoa from the cauda of the epididymis of the hamster and rat were incubated with [5-(3)H]uridine and glucose. By using a procedure avoiding bacterial and other cellular contamination, sonic extracts were prepared and digested with deoxyribonuclease and Pronase. Radioactive RNA of high molecular weight was isolated by two methods: (a) gel filtration on Sephadex G-75 columns and (b) polyacrylamide-gel electrophoresis in which it migrated in the region of 28S and 23S RNA markers. The macromolecules were alkali-labile and hydrolysed by ribonuclease. From (3)H radioactivity and E(260) of the isolated RNA the rate of incorporation of uridine into RNA of spermatozoa was calculated to be 0.1-0.5nmol/h per mg of RNA.
D000818:Animals; D002850:Chromatography, Gel; D006224:Cricetinae; D003851:Deoxyribonucleases / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D066298:In Vitro Techniques; D008297:Male; D008970:Molecular Weight; D010710:Phosphates / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D011402:Pronase / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis*; D051381:Rats; D012260:Ribonucleases / Q000378:metabolism; D013094:Spermatozoa / Q000378:metabolism*; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D010710:Phosphates; D010761:Phosphorus Radioisotopes; D014316:Tritium; D012313:RNA; D003851:Deoxyribonucleases; D012260:Ribonucleases; D011402:Pronase; D005947:Glucose; D014529:Uridine