Protein and fatty acid composition of mesosomal vesicles and plasma membranes of Staphylococcus aureus.
Qualitatively, the protein and fatty acid composition of purified mesosomal vesicles and the plasma membrane isolated from Staphylococcus aureus ATCC 6538P are identical, the major difference between these two cellular components being only quantitative in nature. Mesosomal vesicles and plasma membranes, when subjected to acidic or neutral disk gel electrophoresis, exhibited more than 22 bands of protein. With urea-acetic acid gels, the plasma membrane had a higher concentration of "slower-migrating proteins" whereas "faster-migrating proteins" predominated in the mesosomal vesicles. With neutral disk gel electrophoresis, mesosomal vesicles exhibited one prominent protein band with an approximate molecular weight of 35,000 and which was four times greater than that found in the corresponding region on gels of the plasma membrane. Finally, fatty acid analyses by capillary column gas chromatography showed that although the fatty acid composition is the same, the fatty acid content in mesosomal vesicles is 48% greater than that of the plasma membrane. The dominant fatty acids in both of these cellular components are the iso and anteiso branched methyl C15, C17, and C19 fatty acids and comprise at least 85% of the total fatty acids extracted. These results show that distinct chemical differences exist between the mesosomal vesicles and the plasma membrane of Staphylococcus aureus.
D001426:Bacterial Proteins / Q000032:analysis* / Q000302:isolation & purification; D002462:Cell Membrane / Q000032:analysis*; D002849:Chromatography, Gas; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate; D013210:Staphylococcus / Q000166:cytology*
D001426:Bacterial Proteins; D005227:Fatty Acids; D012967:Sodium Dodecyl Sulfate