Monoclonal production of both IgM and IgG1 antihapten antibody.
The anti-DNP antibodies produced by primary and secondary splenic foci were analyzed for heavy chain class by a radioimmunoassay, using iodinated, purified goat antimouse micro-chain antibody and goat antimouse gamma1 chain antibody. The frequency of primary and secondary foci producing both IgM and IgG1 anti-DNP antibody (16% and 14%, respectively) was considerably higher than that which would be predicted by a random distribution. It would thus appear that IgM and IgG1 antibody can be made by the clonal progeny of a single precursor cell.
D000818:Animals; D000917:Antibody Formation; D000921:Antibody-Producing Cells; D002999:Clone Cells; D004140:Dinitrophenols; D006041:Goats / Q000276:immunology; D006241:Haptens; D006433:Hemocyanins; D007106:Immune Sera; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D051379:Mice; D008807:Mice, Inbred BALB C; D011863:Radioimmunoassay; D013154:Spleen / Q000276:immunology
D004140:Dinitrophenols; D006241:Haptens; D007106:Immune Sera; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D006433:Hemocyanins