Processing of adenovirus 2-induced proteins.
Analysis of (35)S-methionine-labeled extracts of adenovirus 2-infected KB cells revealed 22 virus-induced polypeptide components. Most proteins of the virion were easily detected in extracts of whole cells labeled for short periods between 15 and 30 h after infection; however, several virion components were conspicuously absent. Radioactivity appeared in two of these virion components during a chase in nonradioactive medium, and this appearance was paralleled by a decrease in the radioactivity associated with two nonvirion adenovirus-induced proteins, results which imply precursor-product relationships for these components. Comparison of one of the chasable adenovirus-induced components (designated P-VII; mass of 20,000 daltons) and the major core protein (VII; mass of 18,500 daltons) of the virion showed that they have four common methionine-containing tryptic peptides; P-VII has an additional methionine residue which is not found in the major core protein. We propose that at least two of the adenovirus 2 virion components are derived by the cleavage of higher molecular weight precursor polypeptides.
D000256:Adenoviridae / Q000032:analysis* / Q000254:growth & development / Q000378:metabolism; D001345:Autoradiography; D002277:Carcinoma; D002460:Cell Line; D002474:Cell-Free System; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008715:Methionine / Q000378:metabolism; D009062:Mouth Neoplasms; D010455:Peptides / Q000032:analysis; D011498:Protein Precursors / Q000096:biosynthesis; D013462:Sulfur Radioisotopes; D013997:Time Factors; D014357:Trypsin; D014764:Viral Proteins / Q000032:analysis* / Q000096:biosynthesis; D014779:Virus Replication
D010455:Peptides; D011498:Protein Precursors; D013462:Sulfur Radioisotopes; D014764:Viral Proteins; D008715:Methionine; D014357:Trypsin