Incorporation of labeled precursors into the RNA and proteins of lactic dehydrogenase virus.
Lactic dehydrogenase virus was grown in primary mouse embryo cells and labeled with (3)H-uridine and (3)H-amino acids. Concentrated and purified virus was banded by isopycnic centrifugation in sucrose gradients, and infectivity and radioactivity were found to correspond at a density of 1.17 g/cm(3). The extracted viral RNA was resolved by electrophoresis in polyacrylamide-agarose mixed gels, and the mol wt was estimated to be 6.0 x 10(6).
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D002499:Centrifugation, Density Gradient; D046508:Culture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D007770:L-Lactate Dehydrogenase; D051379:Mice; D008970:Molecular Weight; D012328:RNA Viruses / Q000032:analysis / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis*; D012260:Ribonucleases; D014316:Tritium; D014529:Uridine / Q000378:metabolism*; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis*; D014779:Virus Replication
D000596:Amino Acids; D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium; D007770:L-Lactate Dehydrogenase; D012260:Ribonucleases; D014529:Uridine