Biosynthesis of glutamic acid in Saccharomyces: accumulation of tricarboxylic acid cycle intermediates in a glutamate auxotroph.
Aconitaseless glutamic acid auxotroph MO-1-9B of Saccharomyces grew in glutamic acid-supplemented minimal medium, but failed to grow when glutamic acid was substituted by proline, arginine, ornithine, or glutamine. This mutant was also unable to utilize lactate or glycerol as a carbon source. Under a glutamic acid-limiting condition, by using acetate-1-(14)C as tracer, the mutant accumulated rather large amounts of (14)C-citric acid and (14)C-succinic acid when compared with the wild-type strain. Under excess glutamic acid supplementation, accumulation of citric acid and succinic acid was considerably reduced. When (14)C-glutamic acid-(U) was used as tracer, (14)C-alpha-ketoglutaric acid, (14)C-citric acid, and (14)C-succinic acid were accumulated in the mutant. The citric acid peak was the largest, followed by alpha-ketoglutaric acid and succinic acid. In the wild-type strain under similar conditions, only small amounts of (14)C-citric acid and (14)C-succinic acid and no (14)C-alpha-ketoglutaric acid were accumulated.
D000085:Acetates / Q000378:metabolism; D031841:Aconitum; D001120:Arginine / Q000378:metabolism; D002250:Carbon Radioisotopes; D002855:Chromatography, Thin Layer; D002951:Citrates / Q000096:biosynthesis*; D002952:Citric Acid Cycle; D003470:Culture Media; D005971:Glutamates / Q000096:biosynthesis*; D005973:Glutamine / Q000378:metabolism; D005977:Glutarates / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006836:Hydro-Lyases / Q000096:biosynthesis; D007656:Ketoglutaric Acids / Q000096:biosynthesis*; D007773:Lactates / Q000378:metabolism; D009154:Mutation; D009952:Ornithine / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D012440:Saccharomyces / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D013053:Spectrophotometry; D013386:Succinates / Q000096:biosynthesis*
D000085:Acetates; D002250:Carbon Radioisotopes; D002951:Citrates; D003470:Culture Media; D005971:Glutamates; D005977:Glutarates; D007656:Ketoglutaric Acids; D007773:Lactates; D013386:Succinates; D005973:Glutamine; D001120:Arginine; D011392:Proline; D009952:Ornithine; D006836:Hydro-Lyases; D005990:Glycerol