Rat liver microsomal palmitoyl-coenzyme A synthetase. Structural properties.
The structural properties of purified palmitoyl-CoA synthetase from rat liver microsomal material were investigated. The active enzyme has a molecular weight of 168000 and contains about 8.2mol of phospholipid bound/mol of enzyme protein, as well as bound fatty acids. Association of the active enzyme was shown to occur, without impairment of catalytic activity. The lowest-molecular-weight species obtained under denaturing conditions was 27000 daltons.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D003066:Coenzyme A Ligases / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D008055:Lipids / Q000032:analysis; D046911:Macromolecular Substances; D008862:Microsomes, Liver / Q000201:enzymology*; D008970:Molecular Weight; D010169:Palmitic Acids; D010743:Phospholipids / Q000032:analysis; D011485:Protein Binding; D011489:Protein Denaturation; D051381:Rats; D012967:Sodium Dodecyl Sulfate; D014316:Tritium; D014461:Ultracentrifugation
D000596:Amino Acids; D008055:Lipids; D046911:Macromolecular Substances; D010169:Palmitic Acids; D010743:Phospholipids; D014316:Tritium; D012967:Sodium Dodecyl Sulfate; D003066:Coenzyme A Ligases