title: Equine herpesviruses: antigenic relationships and deoxyribonucleic acid densities.
abstract: Equine herpesviruses with a deoxyribonucleic acid density of 1.716 to 1.717 g/cm(3) were compared with one another by the plaque-reduction test and by the rate of development of cytopathic effect as indicated by plaque size in rabbit kidney cultures. Of the 19 isolates studied, the 9 which had already been tentatively labeled equine abortion viruses were serologically similar to one another; each of them grew more quickly than did any of the other 10 isolates although the mean plaque sizes formed a series of gradations with no clear hiatus which would permit the unequivocal delineation of the abortion viruses from the slowly growing strains. The 10 slowly growing isolates showed antigenic heterogeneity even though complement was present; the neutralizing capacity of an antiserum against the heterologous strains was, in most instances, markedly less than against the homologous strains, the range of the 50% endpoints being much greater than that observed among the equine abortion viruses, or among isolates of herpes simplex type 1. There was no cross neutralization between the equine abortion viruses and any of the 10 slowly growing isolates. An extra band of deoxyribonucleic acid, at 1.723 to 1.725 g/cm(3), was present in two of the slowly growing strains when originally grown in rabbit cells, but was no longer present after passage in cat cells. This band occupied the same position as one reported in the hamster-passaged strain of equine abortion virus, and had a density similar to that of the equine genital herpesvirus. Although the taxonomic demarcation of the equine abortion viruses and the slowly growing herpesviruses from one another is still open to question, they can be conveniently labeled equine herpesviruses 1 and 2, respectively; the genital virus would be termed equine herpesvirus 3.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000032:analysis*; D003720:Densitometry; D006564:Herpesviridae / Q000276:immunology*; D006736:Horses / Q000276:immunology*; D007106:Immune Sera; D007668:Kidney / Q000382:microbiology; D009500:Neutralization Tests; D011817:Rabbits / Q000276:immunology; D010948:Viral Plaque Assay; D014776:Virus Cultivation
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral; D007106:Immune Sera