Zinc requirement for DNA replication in stimulated human lymphocytes.
The requirement for Zn(++) in DNA replication by phytohemagglutinin-stimulated human lymphocytes was studied. When 6 microM o-phenanthroline, a chelator with a high affinity for Zn(++), is added to cultures of stimulated lymphocytes a nearly complete inhibition of thymidine incorporation results within a few hours. In contrast, the incorporation of uridine is only slightly reduced and the incorporation of leucine is unaffected. m-Phenanthroline, a nonchelating analogue, does not alter the rate of thymidine incorporation even when present in 10-fold greater amounts than o-phenanthroline. The inhibition of thymidine incorporation by o-phenanthroline could be entirely reversed by the addition of Zn(++) to the cultures, or could be prevented by the prior addition of either Zn(++) or Ni(++). All other divalent cations tested were incapable of reversing the o-phenanthroline inhibition of thymidine incorporation.
D001798:Blood Proteins / Q000096:biosynthesis; D002413:Cations, Divalent; D002478:Cells, Cultured; D004261:DNA Replication; D003841:Deoxycytidine / Q000378:metabolism; D006801:Humans; D037102:Lectins; D007930:Leucine / Q000378:metabolism; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D009532:Nickel / Q000494:pharmacology; D010618:Phenanthrolines / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D015032:Zinc / Q000494:pharmacology / Q000502:physiology*
D001798:Blood Proteins; D002413:Cations, Divalent; D037102:Lectins; D010618:Phenanthrolines; D003841:Deoxycytidine; D014316:Tritium; D012313:RNA; D009532:Nickel; D007930:Leucine; D015032:Zinc; D013936:Thymidine; D014529:Uridine