title: Production of the hepatotoxic chlorine-containing peptide by Penicillium islandicum Sopp.
abstract: Twenty-six strains of Penicillium islandicum Sopp were tested for production of a chlorine-containing peptide (Cl-peptide), which is a hepatotoxin. Highest levels of the mycotoxin were produced in a modification of Wickerham medium. The mycotoxin was isolated by adsorption on charcoal and, after washing the charcoal with acetone-water (1:1), was eluted into n-butanol. Further purification was accomplished by gel filtration. Maximum yields were 10 to 20 mg of toxin per liter of culture filtrate.
mesh_terms: D000327:Adsorption; D000818:Animals; D000440:Butanols; D002606:Charcoal; D002713:Chlorine; D002850:Chromatography, Gel; D003470:Culture Media; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D009183:Mycotoxins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000633:toxicity; D010407:Penicillium / Q000032:analysis / Q000378:metabolism*; D010452:Peptide Biosynthesis; D010455:Peptides / Q000302:isolation & purification / Q000633:toxicity; D013045:Species Specificity
chemical_list: D000440:Butanols; D003470:Culture Media; D009183:Mycotoxins; D010455:Peptides; D002606:Charcoal; D002713:Chlorine