A method for the simultaneous isolation of factor X and prothrombin from bovine plasma.
1. A method is described for the simultaneous isolation of both Factor X and prothrombin from bovine plasma. The proteins are adsorbed on and eluted from barium sulphate and chromatographed on DEAE-Sephadex and are finally purified by rechromatography on DEAE-Sephadex. 2. The proteins can be purified in 48h from the collection of the blood and the method can be used to process large volumes of plasma. 3. The prothrombin has a molecular weight of 70300; the Factor X, on the other hand, is polydisperse, with most of the protein (86%) having a molecular weight of 56000.
D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005170:Factor X / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D008722:Methods; D008970:Molecular Weight; D011516:Prothrombin / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors; D014461:Ultracentrifugation
D011516:Prothrombin; D005170:Factor X