Proteins from human cerebrospinal fluid: binding with nucleic acids.
Cerebrospinal fluid (CSF) contains two groups of proteins that bind tightly to DNA and to polyriboguanylic acid, respectively. In certain diseases the amounts of a given nucleic acid bound by a constant volume of CSF may increase, while in others the amount of such proteins may be reduced. Binding of polyriboguanylic acid increased in CSF samples from patients with brain tumors, stroke, multiple sclerosis, and communicating hydrocephalus, but it significantly decreased in CSF samples from patients with obstructive hydrocephalus. These increases may or may not be proportional to the rise in total CSF proteins characteristic for these diseases. Elevated binding of DNA was observed in samples from patients with hydrocephalus, epilepsy, and cortical atrophy. The technique described may be applicable to the diagnosis of a variety of diseases of the central nervous system.
D000437:Alcoholism / Q000134:cerebrospinal fluid; D001254:Astrocytoma / Q000134:cerebrospinal fluid; D001927:Brain Diseases / Q000134:cerebrospinal fluid*; D001930:Brain Injuries / Q000134:cerebrospinal fluid; D001932:Brain Neoplasms / Q000134:cerebrospinal fluid*; D002277:Carcinoma / Q000134:cerebrospinal fluid; D002556:Cerebrospinal Fluid Proteins / Q000378:metabolism*; D002561:Cerebrovascular Disorders / Q000134:cerebrospinal fluid; D002675:Child, Preschool; D004247:DNA / Q000378:metabolism*; D004827:Epilepsy / Q000134:cerebrospinal fluid; D005260:Female; D006150:Guanine Nucleotides / Q000378:metabolism*; D006261:Headache / Q000134:cerebrospinal fluid; D006801:Humans; D006849:Hydrocephalus / Q000134:cerebrospinal fluid; D008579:Meningioma / Q000134:cerebrospinal fluid; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid; D009442:Neurilemmoma / Q000134:cerebrospinal fluid; D010761:Phosphorus Radioisotopes; D011119:Polynucleotides / Q000378:metabolism*; D011485:Protein Binding; D012559:Schizophrenia / Q000134:cerebrospinal fluid; D014316:Tritium
D002556:Cerebrospinal Fluid Proteins; D006150:Guanine Nucleotides; D010761:Phosphorus Radioisotopes; D011119:Polynucleotides; D014316:Tritium; D004247:DNA