Prekallikrein deficiency in man.
Blood plasma obtained from an individual with abnormal thromboplastin formation, due to deficiency of Fletcher factor, was fully corrected by 2% of normal, Hageman factor- or PTA-deficient plasma. It was also reconstituted by addition of highly purified human or rabbit prekallikrein. The plasma failed to generate kinin upon exposure to kaolin, a defect which was also corrected by addition of prekallikrein. Prekallikrein antigen was not detectable in this plasma. Fletcher factor-deficient plasma did not support the normal generation of PF/dil when dilute plasma was incubated in glass vessels and injected intracutaneously. Small quantities of Fletcher factor-deficient or Hageman factor-deficient plasma corrected the ability of the other to generate PF/dil. The formation of plasmin in dilute, acidified plasma incubated with kaolin was also abnormal in Fletcher factor-deficient plasma. Plasmin generation was normalized by addition of prekallikrein or small quantities of Hageman factor-deficient plasma. The data support the identity of Fletcher factor and prekallikrein.
D001778:Blood Coagulation Disorders / Q000097:blood* / Q000201:enzymology; D001779:Blood Coagulation Factors / Q000032:analysis; D004792:Enzyme Precursors / Q000097:blood*; D006801:Humans; D007610:Kallikreins / Q000097:blood*; D013925:Thromboplastin
D001779:Blood Coagulation Factors; D004792:Enzyme Precursors; D013925:Thromboplastin; D007610:Kallikreins