Automated cation-exchange chromatography of peptides at a high sensitivity. (0-0.1 Absorbance range).
A method is described where leaching of the column resin by pyridine-acetate buffers is minimal and excessive rise of the base-line of the chromatogram is avoided. The method can be used to fractionate peptide mixtures at a concentration of 2-20nmol/component.
D000085:Acetates; D001322:Autoanalysis; D002021:Buffers; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003956:Dialysis; D005340:Fibrinogen / Q000032:analysis; D006801:Humans; D066298:In Vitro Techniques; D008722:Methods; D010455:Peptides / Q000302:isolation & purification*; D011725:Pyridines
D000085:Acetates; D002021:Buffers; D010455:Peptides; D011725:Pyridines; D005340:Fibrinogen