Hyocholic acid as internal standard for quantitation of human fecal bile acids.
After hyocholate is added to a fecal homogenate, the bile acids are extracted, purified by thin-layer chromatography, and quantitated by gas-liquid chomatography as methyl ester trifluoroacetates on QF-1 columns.
D001647:Bile Acids and Salts / Q000032:analysis*; D002250:Carbon Radioisotopes; D002635:Chenodeoxycholic Acid / Q000032:analysis; D002793:Cholic Acids; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D003840:Deoxycholic Acid / Q000032:analysis; D005243:Feces / Q000032:analysis*; D006801:Humans; D008722:Methods; D014316:Tritium
D001647:Bile Acids and Salts; D002250:Carbon Radioisotopes; D002793:Cholic Acids; D003840:Deoxycholic Acid; D002635:Chenodeoxycholic Acid; D014316:Tritium