Isolation of group H streptococcal competence factor.
Competence factor (CF) was purified 600-fold from culture filtrates of competent group H streptococcus, strain Challis, by fractionation on a carboxymethyl-Sephadex column. The preparation did not contain orcinol- or diphenylamine-positive material, and CF activity was destroyed by trypsin. An examination of its chemical composition revealed 10 amino acids, muramic acid, glucosamine, and glucose.
D000596:Amino Acids / Q000032:analysis; D001426:Bacterial Proteins / Q000032:analysis*; D002849:Chromatography, Gas; D003470:Culture Media; D005612:Freeze Drying; D013291:Streptococcus / Q000032:analysis*
D000596:Amino Acids; D001426:Bacterial Proteins; D003470:Culture Media