title: Carbohydrate metabolism in Agrobacterium tumefaciens.
abstract: The activity of pentose cycling (PC) reactions in Agrobacterium tumefaciens is much greater than that normally found in bacteria, and in this regard the organism represents a unique category. Equations specifically derived from radiorespirometric data for bacteria with high PC activity in the presence of an alternate pathway are presented. A. tumefaciens utilizes d-glucose by strictly aerobic mechanisms involving the Entner-Doudoroff (ED) and PC pathways; relative participation by the ED pathway is 55% and by the PC cycle, 44%. The 3-ketoglycose-synthesizing system in the bacterium does not affect the relative participation of these two pathways. Radiorespirometric and enzymatic analyses clearly demonstrate that the Embden-Meyerhof-Parnas pathway does not function. Studies on the oxidation of pyruvic, acetic, succinic, and glutamic acids show that terminal respiration includes both the tricarboxylic acid and glyoxylic acid cycles.
mesh_terms: D000085:Acetates / Q000378:metabolism; D000332:Aerobiosis; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000446:Aldehyde-Lyases / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002245:Carbon Dioxide / Q000096:biosynthesis; D002250:Carbon Radioisotopes; D002474:Cell-Free System; D002952:Citric Acid Cycle; D005947:Glucose / Q000378:metabolism; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism; D012231:Rhizobium / Q000201:enzymology / Q000378:metabolism*; D013386:Succinates / Q000378:metabolism; D014166:Transferases / Q000378:metabolism
chemical_list: D000085:Acetates; D002250:Carbon Radioisotopes; D005971:Glutamates; D011773:Pyruvates; D013386:Succinates; D002245:Carbon Dioxide; D000429:Alcohol Oxidoreductases; D014166:Transferases; D010770:Phosphotransferases; D000446:Aldehyde-Lyases; D005956:Glucose-6-Phosphate Isomerase; D005947:Glucose; D005990:Glycerol