Replication process of the parvovirus H-1. I. Kinetics in a parasynchronous cell system.
Parasynchronous cultures of hamster embryo cells were used to study some of the events in the replication process of the parvovirus H-1. Synthesis of viral DNA, viral hemagglutinating antigen, and infectious virus were examined. It was found that initiation of DNA synthesis, on which subsequent viral hemagglutinin synthesis was dependent, occurred at a specific time in late S-phase. Production of H-1 viral protein was shown to be sensitive to inhibition by alpha-amanitin. The implications of these findings are discussed.
D000818:Animals; D001345:Autoradiography; D002460:Cell Line; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D003561:Cytarabine / Q000494:pharmacology; D004267:DNA Viruses / Q000254:growth & development* / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis; D003609:Dactinomycin / Q000494:pharmacology; D006389:Hemagglutinins, Viral; D009183:Mycotoxins / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
D004279:DNA, Viral; D006389:Hemagglutinins, Viral; D009183:Mycotoxins; D014764:Viral Proteins; D003561:Cytarabine; D014316:Tritium; D003609:Dactinomycin; D013936:Thymidine