The presence of N-terminal methionine on nascent protein of rat liver and rabbit reticulocytes and its cleavage during polypeptide-chain elongation.
1. The size of nascent globin peptides from which the N-terminal methionine residue is cleaved has been investigated by comparing the proportion of N-terminal methionine and valine in short and long chains. Nascent chains were labelled in rabbit reticulocyte lysates, fractionated according to length by chromatography on Sephadex G-50, and analysed by the Edman degradation of selected pooled fractions. It was found that different peptide fractions contained either methionine or valine, but not both, as the N-terminal residue. Methionine was present at the N-terminus of globin chains containing up to approx. 50 amino acids whereas valine was found to be the N-terminal amino acid of longer peptides. 2. In similar experiments with nascent proteins of rat liver, labelled either in vivo or in a cell-free system containing microsomal material and cell sap, evidence was obtained for the presence of methionine at the N-terminus of nascent chains up to approx. 65 amino acid residues long. Thus protein synthesis in liver appears to be initiated also by methionine, but in this case cleavage takes place somewhat later during peptide elongation than in globin synthesis.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002250:Carbon Radioisotopes; D002845:Chromatography; D003513:Cycloheximide; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism*; D008862:Microsomes, Liver / Q000032:analysis; D010441:Peptide Chain Elongation, Translational; D010443:Peptide Chain Termination, Translational; D010454:Peptide Termination Factors; D014176:Protein Biosynthesis; D011817:Rabbits; D051381:Rats; D012156:Reticulocytes / Q000378:metabolism*; D014316:Tritium; D014443:Tyrosine / Q000378:metabolism
D000596:Amino Acids; D002250:Carbon Radioisotopes; D010454:Peptide Termination Factors; D014316:Tritium; D014443:Tyrosine; D003513:Cycloheximide; D008715:Methionine