Quantitative characteristics of glutamate transport in rat liver mitochondria.
1. The kinetics of glutamate transport into mitochondria were determined by using Bromocresol Purple to terminate the transport process. 2. Glutamate transport was found to have a V(max.) of 9.1nmol/min per mg of protein at pH6.9 and 20 degrees C; the K(m) for glutamate was 4mm. 3. The rate of glutamate deamination in intact mitochondria was tenfold slower than in disrupted mitochondria. 4. These results suggest that glutamate deamination may be controlled by the rate of glutamate transport. Possible consequences of these findings are discussed.
D000586:Amination; D000641:Ammonia / Q000032:analysis; D000818:Animals; D001224:Aspartic Acid / Q000032:analysis; D001692:Biological Transport; D001966:Bromine; D002250:Carbon Radioisotopes; D004396:Coloring Agents; D003408:Cresols; D005971:Glutamates / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008930:Mitochondria, Liver / Q000378:metabolism*; D051381:Rats; D013395:Sucrose / Q000378:metabolism; D014105:Tosyl Compounds; D014316:Tritium; D014867:Water / Q000378:metabolism
D002250:Carbon Radioisotopes; D004396:Coloring Agents; D003408:Cresols; D005971:Glutamates; D014105:Tosyl Compounds; D014867:Water; D014316:Tritium; D001224:Aspartic Acid; D013395:Sucrose; D000641:Ammonia; D001966:Bromine