title: Production of volatile nitrogenous compounds from the degradation of streptomycin by Pseudomonas maltophilia.
abstract: Ammonia, methylamine, and pyridine were detected in broth filtrates of a streptomycin-degrading strain of Pseudomonas maltophilia during growth on streptomycin as a sole carbon and nitrogen source. Ammonia and methylamine, quantitatively measured by conversion to chromophores with picryl sulfonic acid, were found to accumulate in broth, whereas pyridine concentration increased in the early stages of streptomycin degradation and then decreased as the degradation of the antibiotic neared completion. Exogenous pyridine was metabolized by washed-cell suspensions. Use of N-streptomycin-methyl-(14)C showed that the methylamine arose from the N-l-glucosamine-methyl moiety of streptomycin. Methylamine was an end product and was not further metabolized by cells.
mesh_terms: D000641:Ammonia / Q000096:biosynthesis*; D001322:Autoanalysis; D002250:Carbon Radioisotopes; D002849:Chromatography, Gas; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D003470:Culture Media; D008715:Methionine / Q000378:metabolism; D008744:Methylamines / Q000096:biosynthesis*; D011549:Pseudomonas / Q000254:growth & development / Q000378:metabolism*; D011725:Pyridines / Q000096:biosynthesis*; D013056:Spectrophotometry, Ultraviolet; D013307:Streptomycin / Q000378:metabolism*
chemical_list: D002250:Carbon Radioisotopes; D003470:Culture Media; D008744:Methylamines; D011725:Pyridines; D000641:Ammonia; D008715:Methionine; D013307:Streptomycin