Identification of prenol intermediates of wall biosynthesis in growing cells of Lactobacillus plantarum.
The incorporation of (14)C-mevalonic acid by Lactobacillus plantarum predominantly into C(55) prenol made it possible to determine the distribution of (14)C-prenol between all its derivatives. In logarithmic-phase cells, 25% of the prenol was free, 31% was as monophosphate, 4% as pyrophosphate, 12% as peptidoglycan precursor, and 28% as glyco-phospho-prenol. The glyco-phospho-prenol contained rhamnose, and probably glucose, galactose, and ribitol phosphate, and it may, therefore, be involved in polysaccharide and teichoic acid biosynthesis. The proportion of free prenol increased, up to 73%, as the cell culture aged. Free prenol was also formed when cells were incubated in buffer. The free prenol was readily reutilized when cells were returned to growth medium.
D002241:Carbohydrates / Q000032:analysis; D002250:Carbon Radioisotopes; D002473:Cell Wall / Q000032:analysis / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D002848:Chromatography, DEAE-Cellulose; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D006868:Hydrolysis; D007778:Lactobacillus / Q000032:analysis* / Q000254:growth & development / Q000378:metabolism; D008055:Lipids / Q000032:analysis* / Q000096:biosynthesis / Q000302:isolation & purification; D008798:Mevalonic Acid / Q000378:metabolism; D010758:Phosphorus / Q000032:analysis; D010761:Phosphorus Radioisotopes; D010867:Pimelic Acids / Q000032:analysis; D013729:Terpenes / Q000032:analysis
D002241:Carbohydrates; D002250:Carbon Radioisotopes; D008055:Lipids; D010761:Phosphorus Radioisotopes; D010867:Pimelic Acids; D013729:Terpenes; D010758:Phosphorus; D008798:Mevalonic Acid