Camphor plasmid-mediated chromosomal transfer in Pseudomonas putida.
Camphor-utilizing strains of Pseudomonas putida have been shown to carry the genetic information required for camphor degradation on a plasmid. The plasmid-carrying strains can serve as donors of both plasmid-borne and chromosomal genes. As recipients, plasmid-deleted strains are much superior to those carrying the camphor pathway genes. The transfer frequency of chromosomal, but not plasmid-borne, genes is markedly enhanced if the donor cells are irradiated with ultraviolet light followed by 3-h of growth on a rich medium in the dark. Recombinants selected for prototrophy are stable and most acquire the camphor (CAM) plasmid concomitantly; only a few of the Cam(+) recombinants inherit the donor's ability to transfer chromosomal genes at a high frequency. Transfer-defective mutations occur on the CAM plasmid, affecting both CAM and chromosomal gene transfer.
D002164:Camphor / Q000502:physiology*; D002876:Chromosomes, Bacterial / Q000502:physiology*; D003470:Culture Media; D005111:Extrachromosomal Inheritance; D005796:Genes / Q000528:radiation effects; D008937:Mitomycins / Q000494:pharmacology; D011549:Pseudomonas / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D011831:Radiation Genetics; D011995:Recombination, Genetic; D013997:Time Factors; D014466:Ultraviolet Rays
D003470:Culture Media; D008937:Mitomycins; D002164:Camphor