title: Purification and properties of an enzyme duet, phosphoribosylaminoimidazole carboxylase and phosphoribosylaminoimidazolesuccinocarboxamide synthetase, involved in the biosynthesis of purine nucleotides de novo.
abstract: Purification and some properties of an adjacent pair of enzymes in the biosynthetic pathway to AMP, phosphoribosylaminoimidazole carboxylase (EC 4.1.1.21) and phosphoribosylaminoimidazolesuccinocarboxamide synthetase (EC 6.3.2.6), are described. The enzymes form a duet, which may be regarded as a single molecule with a dual function, or as two very similar substances.
mesh_terms: D000249:Adenosine Monophosphate / Q000096:biosynthesis*; D000818:Animals; D001224:Aspartic Acid; D002262:Carboxy-Lyases / Q000302:isolation & purification*; D002645:Chickens; D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D007093:Imidazoles; D008099:Liver / Q000201:enzymology; D010428:Pentosephosphates; D010453:Peptide Synthases / Q000302:isolation & purification*
chemical_list: D007093:Imidazoles; D010428:Pentosephosphates; D001224:Aspartic Acid; D000249:Adenosine Monophosphate; D002262:Carboxy-Lyases; C022378:phosphoribosylaminoimidazole carboxylase; D010453:Peptide Synthases