Pyrimidine starvation induced by adenosine in fibroblasts and lymphoid cells: role of adenosine deaminase.
In the presence of 10(-4) to 10(-5) molar adenosine, established cell lines of fibroblastic or lymphoid origin die of pyrimidine starvation. Less than lethal concentrations inhibit cell growth. Over a broad concentration range, the effects of adenosine are prevented by providing a suitable pyrimidine source. We suggest that the recently described immune deficiency disease associated with absence of adenosine deaminase may be the result of pyrimidine starvation induced by adenosine nucleotides in cells of the lymphoid system.
D000241:Adenosine / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000244:Adenosine Diphosphate / Q000096:biosynthesis; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000293:Adolescent; D000619:Aminohydrolases / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D003597:Cytosine Nucleotides / Q000096:biosynthesis; D005260:Female; D005347:Fibroblasts; D006150:Guanine Nucleotides / Q000096:biosynthesis; D006160:Guanosine Triphosphate / Q000096:biosynthesis; D006801:Humans; D007244:Infectious Mononucleosis; D008214:Lymphocytes; D008223:Lymphoma; D051379:Mice; D011742:Pyrimidine Nucleotides / Q000096:biosynthesis*; D014500:Uracil Nucleotides / Q000096:biosynthesis; D014529:Uridine / Q000494:pharmacology
D003597:Cytosine Nucleotides; D006150:Guanine Nucleotides; D011742:Pyrimidine Nucleotides; D014500:Uracil Nucleotides; D000244:Adenosine Diphosphate; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D000619:Aminohydrolases; D000241:Adenosine; D014529:Uridine