The role of sodium pump in the inhibition of smooth muscle responsiveness to agonists during potassium restoration.
1. Isometric contractions of cat splenic capsular smooth muscle in response to noradrenaline and histamine were recorded.2. Removal of potassium from the bathing medium did not change the resting tension or the responsiveness to noradrenaline. Restoration of potassium inhibited responses to noradrenaline or histamine only if the muscles were stimulated with an agonist while in the K-free medium.3. This inhibition of responses to the agonists due to potassium was reversed rapidly by removing the ion or reversed slowly by prolonged exposure to the ion. The inhibition was also blocked by procedures or agents which block the sodium pump (ouabain, substitution of NaCl by LiCl), inhibit active processes (low ambient temperature) or prevent intracellular accumulation of sodium (substitution of choline for sodium).4. It is proposed that under special circumstances such as when there is an increase in internal sodium concentration, the sodium pump is probably electrogenic and causes relaxation when activated by external potassium. In the normal muscle the pump is probably electrically neutral.
D000818:Animals; D002415:Cats; D002794:Choline / Q000494:pharmacology; D005260:Female; D006632:Histamine / Q000494:pharmacology*; D066298:In Vitro Techniques; D008094:Lithium / Q000494:pharmacology; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects* / Q000378:metabolism*; D009638:Norepinephrine / Q000494:pharmacology*; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology*; D011189:Potassium Chloride / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D013154:Spleen / Q000187:drug effects; D013696:Temperature
D012965:Sodium Chloride; D010042:Ouabain; D011189:Potassium Chloride; D006632:Histamine; D008094:Lithium; D012964:Sodium; D002794:Choline; D011188:Potassium; D009638:Norepinephrine