title: The interaction of 3-deoxy-3-fluoro-D-glucose with the hexose-transport system of the human erythrocyte.
abstract: 1. By using an optical method the kinetic parameters of hexose transport across the human erythrocyte membrane were determined for several sugars. The series of half-saturated constants is as follows: 3-deoxy-3-fluoro-d-glucose = 3-O-methyl-d-glucose <d-glucose<d-mannose <3-deoxy-d-glucose<d-galactose<l-arabinose. 2. Estimations of the dissociation energy of the 3-deoxy-3-fluoro-d-glucose-carrier and d-glucose-carrier complexes suggest that the binding of the two sugars to the transport system is equivalent. 3. Incubation of the erythrocytes with 3-deoxy-3-fluoro-d-glucose results in a small but significant release of F(-) anion. Cells treated in this way lose their ability to transport glucose.
mesh_terms: D001089:Arabinose / Q000097:blood; D001693:Biological Transport, Active; D001786:Blood Glucose / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003837:Deoxy Sugars / Q000097:blood; D003847:Deoxyglucose / Q000031:analogs & derivatives; D004912:Erythrocytes / Q000378:metabolism*; D005461:Fluorine / Q000097:blood; D005690:Galactose / Q000097:blood; D006019:Glycolysis; D006454:Hemoglobins / Q000378:metabolism; D006601:Hexoses / Q000097:blood*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008358:Mannose / Q000097:blood; D008759:Methylglycosides / Q000097:blood; D013053:Spectrophotometry; D013329:Structure-Activity Relationship; D013696:Temperature
chemical_list: D001786:Blood Glucose; D003837:Deoxy Sugars; D006454:Hemoglobins; D006601:Hexoses; D008759:Methylglycosides; C007253:3-deoxy-3-fluoro-D-glucose; D005461:Fluorine; D003847:Deoxyglucose; D001089:Arabinose; D008358:Mannose; D005690:Galactose