Ribonucleic acid synthesis in porphyric rat liver induced by 3,5-dicarbethoxy-1,4-dihydrocollidine.
1. Administration of 3,5-dicarbethoxy-1,4-dihydrocollidine to rats caused a marked increase in the activity of delta-aminolaevulinic acid synthetase as well as a slight net increase in RNA in the livers. 2. 3,5-Dicarbethoxy-1,4-dihydrocollidine primarily stimulated the synthesis of RNA in the nucleus of the liver cell. 3. The decrease in RNA synthesis after administration of this drug resulted in a rapid decrease in the activity of this induced enzyme. This was also confirmed by treatment with actinomycin D. 4. From kinetic experiments on synthesis of RNA and delta-aminolaevulinic acid synthetase in vivo the induction mechanism of this enzyme was discussed.
D000624:5-Aminolevulinate Synthetase / Q000096:biosynthesis; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D003998:Dicarboxylic Acids; D004790:Enzyme Induction; D007700:Kinetics; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D009963:Orotic Acid / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D011132:Polyribosomes / Q000378:metabolism; D011164:Porphyrias / Q000139:chemically induced / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D011725:Pyridines; D012313:RNA / Q000096:biosynthesis*; D051381:Rats; D013997:Time Factors
D003998:Dicarboxylic Acids; D010761:Phosphorus Radioisotopes; D011506:Proteins; D011725:Pyridines; D003609:Dactinomycin; D009963:Orotic Acid; D012313:RNA; D004247:DNA; D000624:5-Aminolevulinate Synthetase