title: Gene dosage and the expression of electrophoretic patterns in heteroploid mouse cell lines.
abstract: Spontaneously transformed mouse cell lines heterozygous for electrophoretic markers have been studied to determine the relationship between gene dosage and phenotype. It is shown that a clone with an electrophoretic pattern for glucosephosphate isomerase of three bands in a ratio of 4A:4AB:1B contains three copies of chromosome 7, which carries the gene for this enzyme. A clone from a different line with a pattern of three bands in a ratio of 1A:6AB:9B for NADP-isocitrate dehydrogenase has four copies of the chromosome carrying this gene or three copies plus a rearrangement which apparently involves this chromosome. These results show that all of the alleles for each enzyme are expressed to an equal extent in these cells.
mesh_terms: D000818:Animals; D002460:Cell Line; D002999:Clone Cells; D004592:Electrophoresis, Starch Gel; D004622:Embryo, Mammalian; D005796:Genes; D005815:Genetic Code; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis; D006579:Heterozygote; D007521:Isocitrate Dehydrogenase / Q000032:analysis; D007621:Karyotyping; D051379:Mice; D008815:Mice, Inbred Strains; D010641:Phenotype; D011123:Polyploidy; D014170:Transformation, Genetic
chemical_list: D007521:Isocitrate Dehydrogenase; D005956:Glucose-6-Phosphate Isomerase