Isolation and characterization of a glycoprotein from human colostrum.
A glycoprotein was isolated from the M-1 acid glycoprotein fraction of human colostrum. It had a molecular weight of 31200 and contained 27% galactose, 21.7% hexosamine, 8.0% fucose and 10.8% sialic acid by weight. The glycoprotein had no absorption maxima in the 240-300nm region, and was virtually free of ABH(O) and M and N blood-group activity. Alkaline borohydride cleavage of the glycoprotein resulted predominantly in the destruction of threonine and galactosamine.
D001894:Borohydrides; D002845:Chromatography; D002848:Chromatography, DEAE-Cellulose; D003126:Colostrum / Q000032:analysis*; D004586:Electrophoresis; D005260:Female; D005643:Fucose / Q000032:analysis; D005688:Galactosamine; D005690:Galactose / Q000032:analysis; D006023:Glycoproteins / Q000302:isolation & purification*; D006595:Hexosamines / Q000032:analysis; D006801:Humans; D008970:Molecular Weight; D009438:Neuraminic Acids / Q000032:analysis; D011247:Pregnancy; D013053:Spectrophotometry; D013912:Threonine; D014461:Ultracentrifugation
D001894:Borohydrides; D006023:Glycoproteins; D006595:Hexosamines; D009438:Neuraminic Acids; D005643:Fucose; D013912:Threonine; D005688:Galactosamine; D005690:Galactose