title: Method for storing Toxoplasma gondii (RH strain) in liquid nitrogen.
abstract: A simple method for storing the trophozoites of Toxoplasma gondii (RH strain) is described. Viability of the parasite was maintained for 160 days.
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide; D005615:Freezing; D005990:Glycerol; D006801:Humans; D008168:Lung; D008722:Methods; D051379:Mice; D009584:Nitrogen; D011309:Preservation, Biological; D013997:Time Factors; D014122:Toxoplasma / Q000254:growth & development / Q000472:pathogenicity
chemical_list: D003451:Cryoprotective Agents; D009584:Nitrogen; D005990:Glycerol; D004121:Dimethyl Sulfoxide