Evidence of the requirement of a full complement of manganese and calcium ions for optimal binding of carbohydrates to electrophoretically homogeneous concanavalin A.
D001322:Autoanalysis; D002118:Calcium / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003208:Concanavalin A / Q000032:analysis* / Q000302:isolation & purification; D003911:Dextrans; D003956:Dialysis; D004589:Electrophoresis, Disc; D004591:Electrophoresis, Polyacrylamide Gel; D005782:Gels; D006863:Hydrogen-Ion Concentration; D008345:Manganese / Q000032:analysis*; D011485:Protein Binding; D013054:Spectrophotometry, Atomic; D013056:Spectrophotometry, Ultraviolet; D014461:Ultracentrifugation
D002241:Carbohydrates; D003911:Dextrans; D005782:Gels; D003208:Concanavalin A; D008345:Manganese; D002118:Calcium