Rosette inhibition test in severely burned patients.
The rosette inhibition test has been advocated as a test of immune reactivity in patients given transplants. We have done the test in lymphocytes from patients with severe burns, non-burnt control patients, and normal subjects. There was no correlation with the period of immunosuppression which follows a severe burn, though both groups of patients showed a wider range of minimal inhibitory concentrations (M.I.C.) than the normal group. Wide day-to-day variation of M.I.C. in burn and control patients was noted; this seemed to correlate with the ratio of sodium to potassium excreted in the urine. In-vitro experiments suggest that the M.I.C. is correlated with a plasma constituent which may be similar in action to hydrocortisone and that the rosette inhibition test is not a direct measure of immune reactivity.
D000410:Alanine Transaminase / Q000652:urine; D000469:Alkaline Phosphatase / Q000652:urine; D000818:Animals; D001219:Aspartate Aminotransferases / Q000652:urine; D002056:Burns / Q000097:blood / Q000276:immunology* / Q000652:urine; D006801:Humans; D006854:Hydrocortisone; D007104:Immune Adherence Reaction; D007106:Immune Sera; D007165:Immunosuppression Therapy; D008214:Lymphocytes / Q000276:immunology*; D011188:Potassium / Q000652:urine; D012756:Sheep / Q000276:immunology; D012964:Sodium / Q000652:urine
D007106:Immune Sera; D012964:Sodium; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D011188:Potassium; D006854:Hydrocortisone