Isolation and identification of minor components of commercial kanamycin.
A fraction enriched in the minor components of the kanamycin complex was prepared from a commercial sample. The enriched fraction was subjected to Amberlite CG-50 and Dowex 1-X2 column chromatography. Two minor components were isolated in addition to the known A, B, and C components. These congeners were identified as paromamine and 6-o-(3-amino-3-deoxy-alpha-d-glucopyranosyl)- deoxystreptamine. The identification was based on degradation and mass spectral studies.
D002845:Chromatography; D002855:Chromatography, Thin Layer; D003511:Cyclohexanols / Q000302:isolation & purification; D003514:Cyclohexylamines / Q000302:isolation & purification; D006595:Hexosamines / Q000302:isolation & purification; D007612:Kanamycin / Q000032:analysis*; D013058:Mass Spectrometry
D003511:Cyclohexanols; D003514:Cyclohexylamines; D006595:Hexosamines; D007612:Kanamycin