The reaction pathway of membrane-bound rat liver mitochondrial monoamine oxidase.
1. A preparation of a partly purified mitochondrial outer-membrane fraction suitable for kinetic investigations of monoamine oxidase is described. 2. An apparatus suitable for varying the O(2) concentration in a spectrophotometer cuvette is described. 3. The reaction catalysed by the membrane-bound enzyme is shown to proceed by a double-displacement (Ping Pong) mechanism, and a formal mechanism is proposed. 4. KCN, NaN(3), benzyl cyanide and 4-cyanophenol are shown to be reversible inhibitors of the enzyme. 5. The non-linear reciprocal plot obtained with impure preparations of benzylamine, which is typical of high substrate inhibition, is shown to be due to aldehyde contamination of the substrate.
D000588:Amines / Q000378:metabolism; D000641:Ammonia; D000818:Animals; D001547:Benzaldehydes / Q000494:pharmacology; D001593:Benzyl Compounds / Q000378:metabolism; D001667:Binding, Competitive; D002499:Centrifugation, Density Gradient; D003486:Cyanides / Q000494:pharmacology; D005260:Female; D066298:In Vitro Techniques; D007700:Kinetics; D008566:Membranes / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000201:enzymology*; D008956:Models, Chemical; D008995:Monoamine Oxidase / Q000378:metabolism*; D008996:Monoamine Oxidase Inhibitors / Q000378:metabolism; D010101:Oxygen Consumption; D051381:Rats; D013053:Spectrophotometry
D000588:Amines; D001547:Benzaldehydes; D001593:Benzyl Compounds; D003486:Cyanides; D008996:Monoamine Oxidase Inhibitors; D000641:Ammonia; D008995:Monoamine Oxidase