Purification and properties of rabbit muscle L-glycerol 3-phosphate dehydrogenase.
A modified procedure has been developed for the purification of rabbit muscle l-glycerol 3-phosphate dehydrogenase. The product of the preparation satisfies all criteria of homogeneity. Some physical properties of the enzyme have been re-investigated. The results suggest that previous preparations may have been contaminated with significant amounts of heavy-molecular-weight protein.
D000818:Animals; D001665:Binding Sites; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003460:Crystallization; D003956:Dialysis; D005993:Glycerolphosphate Dehydrogenase / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008970:Molecular Weight; D009132:Muscles / Q000201:enzymology*; D011817:Rabbits; D013053:Spectrophotometry; D015032:Zinc / Q000032:analysis
D005993:Glycerolphosphate Dehydrogenase; D015032:Zinc