Separation and detection of tetracyclines by high-speed liquid chromatography.
The high-speed liquid chromatographic behavior of 15 tetracyclines on anion- and cation-exchange columns is described and illustrated by typical separations of a number of tetracycline derivatives on several systems, as well as by the resolution of tetracycline from its degradation products epi-, epianhydro-, and anhydrotetracycline. These serve as a basis for a rapid, convenient, and precise method for the direct detection of the tetracycline antibiotics and their degradation products.
D002852:Chromatography, Ion Exchange; D008722:Methods; D013752:Tetracycline / Q000032:analysis* / Q000302:isolation & purification
D013752:Tetracycline