title: Equine abortion (herpes) virus: evaluation of markers in a field vaccination trial.
abstract: Twelve mares were vaccinated with attenuated equine abortion virus (EAV) strain RAC-H. Two nonvaccinated mares served as controls. In at least three mares the vaccination appeared to coincide with a natural infection. This was indicated by characterization of the EAV isolated from nasal secretions of six vaccinated mares, a nonvaccinated control, and also from the lung, spleen, and liver of a fetus aborted by a vaccinated mare. The relative sensitivity of the isolated EAV to dithiothreitol was used to distinguish the RAC-H strain and wild-type virus. Of the 10 EAV isolates, four were recognized as being the vaccine strain while six were recognized as being wild-type strains. Three of the latter originated from two vaccinated mares and a nonvaccinated control, and three were recovered from the fetus. The ability of the EAV strains to form plaques in a cloned line of L cells proved to be unsuitable for use as a marker in this study.
mesh_terms: D000034:Abortion, Veterinary / Q000382:microbiology*; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D002478:Cells, Cultured; D004229:Dithiothreitol / Q000494:pharmacology; D005260:Female; D006564:Herpesviridae / Q000187:drug effects / Q000276:immunology*; D006734:Horse Diseases / Q000517:prevention & control*; D006736:Horses; D009500:Neutralization Tests; D011247:Pregnancy; D011817:Rabbits / Q000276:immunology; D014611:Vaccination; D010948:Viral Plaque Assay
chemical_list: D000914:Antibodies, Viral; D004229:Dithiothreitol