Labeling of antibodies with 3H-acetate.
Antibodies can be labeled with (3)H-iodoacetate at pH 9.8 without significant loss of biological activity, and the labeled antibody can be used to detect viral antigens.
D000085:Acetates; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000937:Antigen-Antibody Reactions; D002499:Centrifugation, Density Gradient; D007136:Immunoglobulins / Q000032:analysis; D007553:Isotope Labeling; D008722:Methods; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D013395:Sucrose; D014316:Tritium; D014780:Viruses / Q000302:isolation & purification
D000085:Acetates; D000914:Antibodies, Viral; D007136:Immunoglobulins; D014316:Tritium; D013395:Sucrose