Biosynthesis of noradrenaline in organ cultured hearts.
In hearts of newborn mice, maintained in organ culture in an in vitro environment, noradrenaline levels were reduced after 24 h but subsequently recovered, reaching at three days values significantly greater than those found at birth. The increased synthesis of noradrenaline was blocked by the inhibitor of tyrosine hydroxylase, alpha-methyl tyrosine methyl ester. The site and control of noradrenaline biosynthesis in cultured newborn hearts are discussed.
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D002854:Chromatography, Paper; D003864:Depression, Chemical; D008781:Methyltyrosines / Q000494:pharmacology; D051379:Mice; D009206:Myocardium / Q000378:metabolism*; D009638:Norepinephrine / Q000096:biosynthesis*; D009924:Organ Culture Techniques; D013997:Time Factors; D014316:Tritium
D008781:Methyltyrosines; D014316:Tritium; D009638:Norepinephrine