Improved thin-layer technique for detection of arginine dihydrolase among the Pseudomonas species.
The detection of arginine dihydrolase among Pseudomonas spp. by thin-layer chromatography is described. The method is based on the procedure of Williams and co-workers (1971) but was improved in sensitivity by selection of a better solvent system.
D001120:Arginine / Q000378:metabolism; D002262:Carboxy-Lyases / Q000032:analysis* / Q000378:metabolism; D002855:Chromatography, Thin Layer; D002956:Citrulline / Q000096:biosynthesis; D006146:Guanidines / Q000096:biosynthesis; D006867:Hydrolases / Q000032:analysis* / Q000378:metabolism; D008722:Methods; D008851:Micropore Filters; D009952:Ornithine / Q000096:biosynthesis; D011549:Pseudomonas / Q000145:classification / Q000201:enzymology* / Q000378:metabolism; D011700:Putrescine / Q000096:biosynthesis; D012997:Solvents; D013045:Species Specificity
D006146:Guanidines; D012997:Solvents; D002956:Citrulline; D001120:Arginine; D009952:Ornithine; D006867:Hydrolases; D002262:Carboxy-Lyases; D011700:Putrescine