Lipid composition of the zoospores of Blastocladiella amersonii.
The zoospores of Blastocladiella emersonii, when derived from cultures grown on solid media, contain about 11% total lipid. This lipid was separated chromatographically on silicic acid into neutral lipid (46.6%), glycolipid (15.8%), and phospholipid (37.6%). Each class was fractionated further on columns of silicic acid, Florisil, or diethylaminoethyl-cellulose, and monitored by thin-layer chromatography. Triglycerides were the major neutral lipids, mono- and diglycosyldiglycerides were the major glycolipids, and phosphatidylcholine and phosphatidylethanolamine were the major phospholipids. Other neutral lipids and phospholipids detected were: hydrocarbons, free fatty acids, free sterols, sterol esters, diglycerides, monoglycerides, lysophosphatidylcholine, lysophosphatidylethanolamine, phosphatidic acid, phosphatidylserine, and phosphatidylinositol. Palmitic, palmitoleic, stearic, oleic, gamma-linolenic, and arachidonic acids were the most frequently occurring fatty acids. When B. emersonii was grown in (14)C-labeled liquid media, lipid again accounted for 11% of both mature plants and zoospores released from them. The composition of the lipid extracted from such plants and spores was also the same; however, it differed markedly from that of the lipid in spores harvested from solid media, consisting of 28.3% neutral lipid, 12.0% glycolipid, and 59.7% phospholipid. The major lipids were again triglycerides for neutral lipids, mono- and diglycosyldiglycerides for glycolipids, and phosphatidyl choline and phosphatidylethanolamine for phospholipids.
D002250:Carbon Radioisotopes; D002845:Chromatography; D002849:Chromatography, Gas; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D003470:Culture Media; D005227:Fatty Acids / Q000032:analysis; D005658:Fungi / Q000032:analysis* / Q000254:growth & development; D005989:Glycerides / Q000032:analysis; D006017:Glycolipids / Q000032:analysis; D006868:Hydrolysis; D008055:Lipids / Q000032:analysis* / Q000302:isolation & purification; D010743:Phospholipids / Q000032:analysis; D013172:Spores, Fungal / Q000032:analysis* / Q000254:growth & development; D013261:Sterols / Q000032:analysis; D014280:Triglycerides / Q000032:analysis
D002250:Carbon Radioisotopes; D003470:Culture Media; D005227:Fatty Acids; D005989:Glycerides; D006017:Glycolipids; D008055:Lipids; D010743:Phospholipids; D013261:Sterols; D014280:Triglycerides