Contamination of free-ribosome fractions by detached previously membrane-bound ribosomes.
A rough-membrane fraction isolated from rat liver by a procedure designed to prevent membrane denaturation was subjected to the gradient treatment normally used to isolate free ribosomes. Under these conditions, at most 20% of the ribosomes were detached from membrane with less than 5% sedimenting into the free-polyribosome pellet.
D000818:Animals; D002462:Cell Membrane; D002499:Centrifugation, Density Gradient; D002501:Centrifugation, Zonal; D005069:Evaluation Studies as Topic; D005260:Female; D008099:Liver / Q000032:analysis / Q000166:cytology; D008722:Methods; D012313:RNA / Q000032:analysis; D051381:Rats; D012270:Ribosomes / Q000032:analysis*; D013347:Subcellular Fractions / Q000032:analysis
D012313:RNA