5-methylcytidylic acid: absence from mitochondrial DNA of frogs and HeLa cells.
The content of 5-methylcytidylic acid in nuclear DNA and mito chondrial DNA of Xenopus laevis and HeLa cells has been determined. Both nuclear DNA's contain 5-methylcytidylic acid. The 5-methylcytidylic acid content of X. laevis DNA is 1.7 mole percent of total nucleotides, and that of HeLc cell DNA is 0.7 mole percent. In neither mitochondrial DNA could any 5-methylcytidylic acid be detected; the limit of sensitivity was judged at below 0.1 mole percent for X. laevis DNA and below 0.05 mole percent for HeLa cell DNA.
D000818:Animals; D001345:Autoradiography; D002467:Cell Nucleus / Q000032:analysis; D002855:Chromatography, Thin Layer; D003597:Cytosine Nucleotides / Q000032:analysis*; D004272:DNA, Mitochondrial / Q000032:analysis*; D005260:Female; D006367:HeLa Cells / Q000032:analysis*; D006801:Humans; D010053:Ovary / Q000032:analysis*; D010761:Phosphorus Radioisotopes; D014981:Xenopus
D003597:Cytosine Nucleotides; D004272:DNA, Mitochondrial; D010761:Phosphorus Radioisotopes