Partial purification of an opiate receptor from mouse brain.
A proteolipid isolated from a lipid extract of mouse brain demonstrates stereospecific binding properties for levorphanol. It is present only in neuronal tissue and most abundant in the rhombencephalon. One component saturates at a concentration corresponding to maximum pharmacologic effect in vivo. The estimated mass is 60,000 daltons per bound opiate molecule.
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D001933:Brain Stem / Q000378:metabolism; D002250:Carbon Radioisotopes; D002531:Cerebellum / Q000378:metabolism; D002725:Chloroform; D002850:Chromatography, Gel; D007668:Kidney / Q000378:metabolism; D007700:Kinetics; D007981:Levorphanol / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008074:Lipoproteins / Q000302:isolation & purification* / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D000432:Methanol; D051379:Mice; D008970:Molecular Weight; D009019:Morphinans / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D009270:Naloxone / Q000494:pharmacology; D011955:Receptors, Drug; D014020:Tissue Extracts; D014316:Tritium
D002250:Carbon Radioisotopes; D008074:Lipoproteins; D009019:Morphinans; D011955:Receptors, Drug; D014020:Tissue Extracts; D014316:Tritium; D007981:Levorphanol; D009270:Naloxone; D002725:Chloroform; D000432:Methanol