Steady-state kinetics of rabbit-intestinal sucrase. Kinetic mechanism, Na+ activation, inhibition by tris(hydroxymethyl)aminomethane at the glucose subsite.
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002250:Carbon Radioisotopes; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D004186:Disaccharidases / Q000378:metabolism*; D004789:Enzyme Activation; D005947:Glucose; D007421:Intestine, Small / Q000187:drug effects / Q000201:enzymology*; D007536:Isomerism; D007700:Kinetics; D008094:Lithium; D008358:Mannose / Q000494:pharmacology; D008433:Mathematics; D009005:Monosaccharides / Q000494:pharmacology; D011485:Protein Binding; D011817:Rabbits; D012964:Sodium / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013393:Sucrase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013997:Time Factors; D014325:Tromethamine / Q000494:pharmacology*
D002250:Carbon Radioisotopes; D009005:Monosaccharides; D014325:Tromethamine; D008094:Lithium; D012964:Sodium; D004186:Disaccharidases; D013393:Sucrase; D005947:Glucose; D008358:Mannose