The fractionation of adenosine-rich oligoribonucleotides on polyethyleneimine-cellulose.
After combined pancreatic and T(1) ribonuclease treatment of RNA, a characteristic series of products of the type A(n)N are obtained, where N can be any of the four ribonucleosides. Depending on whether phosphatase treatment is used before the addition of labelled phosphate at the 5'-terminus with bacteriophage phosphokinase, the labelled oligonucleotides obtained may or may not possess a phosphate group at the 3'- as well as the 5'-end. The behaviour of these characteristic products after electrophoresis on cellulose acetate strips followed by chromatography on polyethyleneimine-cellulose thin-layer plates was examined.
D000227:Adenine Nucleotides; D000469:Alkaline Phosphatase; D001483:Base Sequence; D002482:Cellulose; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D004586:Electrophoresis; D005069:Evaluation Studies as Topic; D008722:Methods; D009841:Oligonucleotides / Q000032:analysis / Q000302:isolation & purification*; D010179:Pancreas / Q000201:enzymology; D010761:Phosphorus Radioisotopes; D010770:Phosphotransferases; D012313:RNA; D012260:Ribonucleases; D012265:Ribonucleotides / Q000032:analysis / Q000302:isolation & purification*
D000227:Adenine Nucleotides; D009841:Oligonucleotides; D010761:Phosphorus Radioisotopes; D012265:Ribonucleotides; D012313:RNA; D002482:Cellulose; D010770:Phosphotransferases; D012260:Ribonucleases; D000469:Alkaline Phosphatase