Thiol groups of normal human immunoglobulin G.
Normal human immunoglobulin G (IgG) was treated with radioactive N-ethylmaleimide in the absence of a reducing agent but in the presence of a denaturing solvent. The sites of reaction were determined by isolation of the radioactive peptides from thermolytic digests of the heavy and light chains. Six radioactive peptides were purified from the digest of the light chain and ten from that of the heavy chain. When sequenced, all the peptides were identical with peptides that would be predicted for the half-cystine residues involved in disulphide bonds. The specific radioactivity of the peptides indicated that the proportion of the half-cystine residues in the reduced form varied from 0.57 to 2.54%. These results indicate that the disulphide bonds of IgG are not completely oxidized. The estimate of 0.2mol of SH group/mol of IgG (Cecil & Stevenson, 1965; Luks & Connell, 1968) can be accounted for if 0.8% of every half-cystine residue of the intrachain bonds was in the reduced form. Variable cysteine residues as have been described in several myeloma proteins must occur extremely infrequently among the immunoglobulins.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002250:Carbon Radioisotopes; D002852:Chromatography, Ion Exchange; D002854:Chromatography, Paper; D003553:Cystine / Q000032:analysis; D004220:Disulfides; D004590:Electrophoresis, Paper; D005033:Ethylmaleimide; D006801:Humans; D007074:Immunoglobulin G; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000032:analysis; D011487:Protein Conformation; D011489:Protein Denaturation; D013053:Spectrophotometry; D013820:Thermolysin
D000596:Amino Acids; D002250:Carbon Radioisotopes; D004220:Disulfides; D007074:Immunoglobulin G; D010446:Peptide Fragments; D003553:Cystine; D013820:Thermolysin; D005033:Ethylmaleimide