Conversion of Dexon (p-dimethylaminobenzenediazo sodium sulfonate) to N, N-dimethyl-p-phenylenediamine by Pseudomonas fragi Bk9.
The metabolism of the fungicide Dexon (p-dimethylaminobenzenediazo sodium sulfonate) by a soil bacterium is reported for the first time. The organism which is capable of using Dexon only by a co-metabolic process was obtained by enrichment culture and was identified as Pseudomonas fragi. The first metabolic product of Dexon was identified as N,N-dimethyl-p-phenylenediamine. The presence of an enzyme, p-dimethylaminobenzenediazo sodium sulfonate reductase, capable of reducing Dexon to N,N-dimethyl-p-phenylenediamine has been demonstrated in the cell-free extracts of the organism. The enzyme is found to be in the soluble fraction and requires dithiothreitol as a reductant.
D001391:Azo Compounds / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002474:Cell-Free System; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D003470:Culture Media; D004229:Dithiothreitol / Q000378:metabolism; D005659:Fungicides, Industrial / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D010655:Phenylenediamines / Q000096:biosynthesis*; D011549:Pseudomonas / Q000201:enzymology / Q000378:metabolism*; D012988:Soil Microbiology; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D014461:Ultracentrifugation
D001391:Azo Compounds; D003470:Culture Media; D005659:Fungicides, Industrial; D010655:Phenylenediamines; D010088:Oxidoreductases; D004229:Dithiothreitol