The K-chains of the immunoglobulin from a continuous culture of human lymphocytes (Daudi) have an unusual molecular size.
Membrane-associated immunoglobulin (M-Ig) was isolated from Daudi cells which had been radioiodinated using lactoperoxidase. This M-Ig was found to have a molecular size on SDS-PAGE of 330,000 daltons. The component protein chains (micro and K) have molecular sizes of 75,000 and 33,000 daltons, respectively. Further studies showed that [(3)H]galactose can be incorporated into K-chains, but that extra protein and not carbohydrate was responsible for the increased molecular size of this molecule.
D000596:Amino Acids / Q000378:metabolism; D002051:Burkitt Lymphoma; D002250:Carbon Radioisotopes; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D005690:Galactose / Q000378:metabolism; D006801:Humans; D007128:Immunoglobulin Fragments / Q000032:analysis*; D007457:Iodine Radioisotopes; D008214:Lymphocytes / Q000276:immunology*; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D014316:Tritium
D000596:Amino Acids; D002250:Carbon Radioisotopes; D007128:Immunoglobulin Fragments; D007457:Iodine Radioisotopes; D010455:Peptides; D014316:Tritium; D005690:Galactose