title: Mycoplasma contamination of cultured amniotic fluid cells: potential hazard to prenatal chromosomal diagnosis.
abstract: Amniotic fluid cell cultures were screened for mycoplasma contamination. Mycoplasma RNA's were observed in more than half the cultures examined. Karyotypic analyses of these contaminated cell cultures revealed a significant increase in chromosomal aberrations. These studies emphasize the need for screening for mycoplasma in cultured amniotic cells.
mesh_terms: D000649:Amniocentesis; D000653:Amniotic Fluid / Q000166:cytology / Q000382:microbiology*; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007621:Karyotyping; D009030:Mosaicism; D009174:Mycoplasma / Q000302:isolation & purification* / Q000378:metabolism; D011247:Pregnancy; D012329:RNA, Bacterial / Q000302:isolation & purification*; D014316:Tritium; D014498:Uracil / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
chemical_list: D012329:RNA, Bacterial; D014316:Tritium; D014498:Uracil; D014529:Uridine