title: Host effect on arbovirus replication: appearance of defective interfering particles in murine cells.
abstract: Serial passage of Semliki Forest virus (SFV) in chicken embryo cells had little effect on SFV yield; however, high multiplicity infection of murine cells with one of the late passage pools (passage 9 SFV) resulted in a virus yield 10- to 20-fold lower than that obtained with earlier passage virus and 80-fold lower than the corresponding yield in chicken cells. This effect was accompanied by a striking decrease in the levels of 42S and 26S RNA and by increased proportions of a small single-stranded viral RNA (molecular weight, 9 x 10(5)) and of a low-molecular-weight replicative form. There was also a reduction in the number of specific membranous structures previously associated with the group A arbovirus replication complex. These results suggested that passage 9 SFV contained defective interfering particles which were detected more readily after one passage in a murine indicator host cell. Identical results were obtained with two different murine cell lines: one a leukemia virus-free clone of AKR cells and the other JLS-V9 cells chronically infected with Rauscher leukemia virus. Host production of RNA tumor virus particles apparently did not affect arbovirus replication.
mesh_terms: D000241:Adenosine / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002642:Chick Embryo; D003609:Dactinomycin / Q000494:pharmacology; D003673:Defective Viruses / Q000254:growth & development*; D004591:Electrophoresis, Polyacrylamide Gel; D007942:Leukemia, Experimental; D051379:Mice; D008970:Molecular Weight; D010761:Phosphorus Radioisotopes; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis; D011925:Rauscher Virus / Q000254:growth & development; D012672:Semliki forest virus / Q000032:analysis / Q000254:growth & development* / Q000378:metabolism; D013045:Species Specificity; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D014762:Viral Interference; D014776:Virus Cultivation; D014779:Virus Replication
chemical_list: D010761:Phosphorus Radioisotopes; D012367:RNA, Viral; D014316:Tritium; D003609:Dactinomycin; D000241:Adenosine; D014529:Uridine