Evidence for the binding of the carcinogen 3-methylcholanthrene to both the purine and the pyrimidine bases of hamster fibroblast deoxyribonucleic acid.
The binding of [(3)H]3-methylcholanthrene to the DNA of hamster fibroblasts was studied by using chemical methods for DNA degradation. DNA depurinated by mild acid hydrolysis released approximately half of the radioactivity at the same rate as the purine bases, but the resulting apurinic acid still contained radioactive carcinogen.
D000818:Animals; D001665:Binding Sites; D002845:Chromatography; D006224:Cricetinae; D046508:Culture Techniques; D004247:DNA; D005347:Fibroblasts; D006868:Hydrolysis; D008748:Methylcholanthrene; D011685:Purine Nucleotides; D011742:Pyrimidine Nucleotides; D014316:Tritium
D011685:Purine Nucleotides; D011742:Pyrimidine Nucleotides; D014316:Tritium; D008748:Methylcholanthrene; D004247:DNA