title: Variation in the in vitro migration of sensitized guinea pig peritoneal exudate cells in the presence of coccidioidin.
abstract: Guinea pigs were immunized by intramuscular injection of arthrospores from the M11 strain of Coccidioides immitis, and the peritoneal exudate cells were harvested 4 to 6 weeks later. After incubation with various concentrations of coccidioidin in tissue culture, the area of all migration was measured. Results of this study indicate that at a critical level, a variance of 0.1 mug of antigen per ml, determined the difference between approximately 88% migration and 5% migration as compared with control cells incubated in the absence of antigen. The concentration of antigen (ASU-9 stock coccidioidin concentrate) required to produce essentially complete inhibition of migration was determined to be 12.5 mug/ml.
mesh_terms: D000818:Animals; D000946:Antigens, Fungal; D002464:Cell Migration Inhibition; D003045:Coccidioides / Q000276:immunology*; D046508:Culture Techniques; D005122:Exudates and Transudates / Q000166:cytology / Q000276:immunology*; D006168:Guinea Pigs; D007962:Leukocytes / Q000276:immunology*; D010537:Peritoneum
chemical_list: D000946:Antigens, Fungal