Ionization of a nitrophenol-containing reporter group at the active site of papain.
D000081:Acetamides; D000478:Alkylation; D000588:Amines; D000596:Amino Acids / Q000032:analysis; D001120:Arginine / Q000378:metabolism; D001565:Benzoates / Q000378:metabolism; D001665:Binding Sites; D001965:Bromides; D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D003545:Cysteine; D004147:Dioxins; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008433:Mathematics; D009596:Nitrophenols; D010206:Papain / Q000032:analysis / Q000378:metabolism*; D011522:Protons; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship; D013438:Sulfhydryl Compounds / Q000378:metabolism
D000081:Acetamides; D000588:Amines; D000596:Amino Acids; D001565:Benzoates; D001965:Bromides; D004147:Dioxins; D009596:Nitrophenols; D011522:Protons; D013438:Sulfhydryl Compounds; D001120:Arginine; D010206:Papain; D003545:Cysteine