Active transport of alcohol in Corynebacterium acetophilum.
The transport of alcohols was studied in Corynebacterium acetophilum, which was isolated as a strain growing well on acetate and ethanol. The transport of ethanol was found to be inducible by ethanol, n-propanol, n-butanol, and acetate, whereas transport of methanol occurred by noninducible passive diffusion. The entry of ethanol into the cells occurred against a concentration gradient and showed saturation kinetics with two K(m) values of 2.4 x 10(-5) M and 6.0 x 10(-5) M. Uptake of ethanol was inhibited by sodium azide, sodium cyanide, 2,4-dinitrophenol, and p-chloromercuribenzoate. The transport of ethanol was competitively inhibited by normal alcohols, but not by iso- or tert-alcohols. From these studies, we concluded that an inducible active alcohol transport system mediates the entry of ethanol, n-propanol, or n-butanol into the cells of C. acetophilum.
D000433:1-Propanol / Q000378:metabolism; D000085:Acetates / Q000378:metabolism; D000438:Alcohols / Q000378:metabolism*; D001345:Autoradiography; D001386:Azides / Q000494:pharmacology; D001693:Biological Transport, Active; D000440:Butanols / Q000378:metabolism; D002250:Carbon Radioisotopes; D002729:Chloromercuribenzoates / Q000494:pharmacology; D002855:Chromatography, Thin Layer; D003352:Corynebacterium / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media; D003486:Cyanides / Q000494:pharmacology; D004058:Diffusion; D004140:Dinitrophenols / Q000494:pharmacology; D000431:Ethanol / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D000432:Methanol / Q000378:metabolism; D013997:Time Factors
D000085:Acetates; D000438:Alcohols; D001386:Azides; D000440:Butanols; D002250:Carbon Radioisotopes; D002729:Chloromercuribenzoates; D003470:Culture Media; D003486:Cyanides; D004140:Dinitrophenols; D000431:Ethanol; D000433:1-Propanol; D005947:Glucose; D000432:Methanol