Cell culture of mammalian endometrium and synthesis of blastokinin in vitro.
Endometrial cells obtained from mature female estrous rabbits can be grown in cell culture with the aid of insulin. These cells, after several days in culture, are capable of synthesizing blastokinin by induction with progesterone for 48 hours.
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism*; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis* / Q000302:isolation & purification; D007328:Insulin; D007705:Kinins / Q000096:biosynthesis* / Q000302:isolation & purification; D011374:Progesterone / Q000494:pharmacology; D011817:Rabbits
D003470:Culture Media; D004967:Estrogens; D006023:Glycoproteins; D007328:Insulin; D007705:Kinins; D011374:Progesterone