Structural features of ring C of 20-oxo steroids and the interaction with cortisone reductase.
Kinetic measurements were made with cortisone reductase (20-dihydrocortisone-NAD(+) oxidoreductase, EC 1.1.1.53) and a series of substrates which differed in shape, size and electronic character in the region adjacent to C-11, C-14 and C-18. Structural changes at C-11 in these substrates resulted in up to 660-fold changes in the apparent K(m) value, up to 200-fold changes in the apparent V(max.) value and up to 800-fold changes in the ratio of these kinetic constants. It is suggested that interactions important for substrate function normally occur between the enzyme and the C ring in the region of C-11, that these interactions arise from so-called hydrophobic forces between the generally hydrophobic C ring portion of the substrate and a hydrophobic region of the enzyme, but that when the substrate contains a polar substituent in this portion of the molecule, then polar interactions with polar moieties of the enzyme can also be important. It is further suggested that the part of the enzyme that interacts with the region of C-11 in the substrate is flexible, and that substrate binding involves at least some degree of induced fit.
D001665:Binding Sites; D002855:Chromatography, Thin Layer; D003348:Cortisone; D006913:Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D007700:Kinetics; D008433:Mathematics; D008968:Molecular Conformation; D011485:Protein Binding; D013329:Structure-Activity Relationship
D006913:Hydroxysteroid Dehydrogenases; D003348:Cortisone