Cyanylation of sulfhydryl groups by 2-nitro-5-thiocyanobenzoic acid. High-yield modification and cleavage of peptides at cysteine residues.
D000409:Alanine; D000596:Amino Acids / Q000032:analysis; D001565:Benzoates; D002250:Carbon Radioisotopes; D055598:Chemical Phenomena; D002621:Chemistry; D003545:Cysteine; D003553:Cystine; D004220:Disulfides; D004590:Electrophoresis, Paper; D005978:Glutathione; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007700:Kinetics; D008722:Methods; D009574:Nitro Compounds; D010084:Oxidation-Reduction; D010455:Peptides / Q000032:analysis; D013053:Spectrophotometry; D013438:Sulfhydryl Compounds; D013861:Thiocyanates
D000596:Amino Acids; D001565:Benzoates; D002250:Carbon Radioisotopes; D004220:Disulfides; D007202:Indicators and Reagents; D009574:Nitro Compounds; D010455:Peptides; D013438:Sulfhydryl Compounds; D013861:Thiocyanates; D003553:Cystine; D005978:Glutathione; D003545:Cysteine; D000409:Alanine