Mutants of group D1 Salmonella carrying the somatic antigen of group A organisms: evidence for the lack of cytidine diphosphate paratose-2-epimerase activity.
The mutant strains of Salmonella durban that possessed O antigen 2, 12 of group A Salmonella were defective in the cytidine diphosphate paratose-2-epimerase activity. The enzyme preparation of the mutant strains catalyzed the conversion of cytidine diphosphate glucose into cytidine diphosphate paratose but not into cytidine diphosphate tyvelose. The defect in the epimerase activity was also confirmed by the use of purified cytidine diphosphate paratose as a substrate. The specificity of dideoxyhexosyl transferase catalyzing the formation of the group-specific determinant is discussed.
D000372:Agglutination Tests; D000942:Antigens, Bacterial; D002238:Carbohydrate Epimerases / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002474:Cell-Free System; D002854:Chromatography, Paper; D003562:Cytidine; D005947:Glucose / Q000378:metabolism; D006602:Hexosyltransferases / Q000378:metabolism; D008070:Lipopolysaccharides / Q000096:biosynthesis; D009154:Mutation; D009702:Nucleoside Diphosphate Sugars / Q000096:biosynthesis; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D012475:Salmonella / Q000201:enzymology / Q000276:immunology*
D000942:Antigens, Bacterial; D002250:Carbon Radioisotopes; D008070:Lipopolysaccharides; D009702:Nucleoside Diphosphate Sugars; D011135:Polysaccharides, Bacterial; D003562:Cytidine; D006602:Hexosyltransferases; D002238:Carbohydrate Epimerases; D005947:Glucose