Formation of an intermediate in prostaglandin biosynthesis and its association with the platelet release reaction.
A compound that could be converted to prostaglandin F(2alpha) by mild chemical reduction was formed by human platelets in response to arachidonic acid, collagen, or L-epinephrine. It was present in maximal amounts at about 1 min after addition of arachidonic acid or collagen to platelet-rich plasma. Its initial formation appeared to precede platelet aggregation by these agents and was closely correlated with the release of adenine nucleotides and radioactive 5-hydroxytryptamine from platelets. Moreover, the compound was itself found outside the platelets. This compound is probably an endoperoxide intermediate in prostaglandin biosynthesis and may be a trigger for the platelet release reaction.
D000227:Adenine Nucleotides / Q000378:metabolism; D001095:Arachidonic Acids / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002250:Carbon Radioisotopes; D003094:Collagen / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D006801:Humans; D010545:Peroxides / Q000096:biosynthesis; D010973:Platelet Adhesiveness / Q000187:drug effects; D011453:Prostaglandins / Q000032:analysis / Q000096:biosynthesis*; D011863:Radioimmunoassay; D012701:Serotonin / Q000378:metabolism; D013997:Time Factors
D000227:Adenine Nucleotides; D001095:Arachidonic Acids; D002250:Carbon Radioisotopes; D010545:Peroxides; D011453:Prostaglandins; D012701:Serotonin; D003094:Collagen; D004837:Epinephrine