Immunoreactive gastrin components in human serum.
The apparent molecular size and charge of immunoreactive gastrin components were studied in sera from patients with pernicious anaemia or gastrinomas (the Zollinger-Ellison syndrome) by Sephadex gel filtration and aminoethylcellulose chromatography. The following serum components were distinguished: (1) a monophasic component I similar in size to proinsulin which was converted into ;little' gastrin I by trypsin digestion; (2) a biphasic component II, corresponding to ;big' gastrins I and II (Gregory and Tracy); (3) a biphasic component III corresponding to ;little' gastrins I and II (Gregory and Tracy); and (4) a biphasic component IV, corresponding to ;minigastrins' I and II (Gregory and Tracy). ;Big, big' gastrin, a plasma component found in the void volume of the Sephadex G-50 column by Yalow and Berson (1972) was undetectable in the sera investigated. A component in gastrinoma and antral mucosa extracts corresponding in size to ;big big' gastrin was detectable by the assay; the ;big big' gastrin fraction from gastrinoma tissue was heterogenous, with components of apparent MW 30 000-100 000. It is concluded that serum gastrin circulates in the form of at least four components, of which the three smaller ones are in pairs.
D000752:Anemia, Pernicious / Q000276:immunology*; D002845:Chromatography; D002850:Chromatography, Gel; D005755:Gastrins / Q000097:blood* / Q000302:isolation & purification; D006801:Humans; D007106:Immune Sera; D007328:Insulin; D007457:Iodine Radioisotopes; D008970:Molecular Weight; D011863:Radioimmunoassay; D011868:Radioisotopes; D012965:Sodium Chloride; D012975:Sodium Isotopes; D014357:Trypsin / Q000378:metabolism; D015043:Zollinger-Ellison Syndrome / Q000276:immunology*
D005755:Gastrins; D007106:Immune Sera; D007328:Insulin; D007457:Iodine Radioisotopes; D011868:Radioisotopes; D012975:Sodium Isotopes; D012965:Sodium Chloride; D014357:Trypsin