A simple and precise assay of the enzymatic conversion of cholesterol into pregnenolone.
D000096:Acetone; D000311:Adrenal Glands / Q000201:enzymology*; D000818:Animals; D002208:Caproates / Q000032:analysis / Q000302:isolation & purification; D002250:Carbon Radioisotopes; D002417:Cattle; D002784:Cholesterol / Q000378:metabolism*; D002845:Chromatography; D003460:Crystallization; D005069:Evaluation Studies as Topic; D007700:Kinetics; D008722:Methods; D008928:Mitochondria / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000302:isolation & purification; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D011284:Pregnenolone / Q000096:biosynthesis*; D013329:Structure-Activity Relationship; D013464:Sulfuric Acids; D014316:Tritium
D002208:Caproates; D002250:Carbon Radioisotopes; D009097:Multienzyme Complexes; D013464:Sulfuric Acids; D014316:Tritium; D000096:Acetone; D011284:Pregnenolone; D002784:Cholesterol; D006899:Mixed Function Oxygenases; D010088:Oxidoreductases