In vitro uptake of 14C-labeled amino acids by preparations of partially purified Anaplasma marginale bodies.
Studies on the incorporation of three (14)C-labeled amino acids into preparations of partially purified Anaplasma marginale were conducted. Relatively high levels of uptake of radioactive isoleucine, glycine, and methionine were detected in both trichloroacetic acid-soluble and -insoluble fractions of these preparations, indicating that the parasite is capable of protein synthesis outside its erythrocytic environment. When erythrocytes (red blood cells) from infected calves were incubated with [(14)C]methionine and Anaplasma bodies were subsequently purified, it was found that some of the label detected was associated with the marginal bodies.
D000596:Amino Acids / Q000378:metabolism*; D000709:Anaplasma / Q000302:isolation & purification / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis; D002250:Carbon Radioisotopes; D004912:Erythrocytes / Q000382:microbiology; D005998:Glycine / Q000378:metabolism; D007532:Isoleucine / Q000378:metabolism; D008715:Methionine / Q000378:metabolism
D000596:Amino Acids; D001426:Bacterial Proteins; D002250:Carbon Radioisotopes; D007532:Isoleucine; D008715:Methionine; D005998:Glycine