title: Some cultural conditions that control biosynthesis of lipid and aflatoxin by Aspergillus parasiticus.
abstract: Synthesis of total lipid and aflatoxin by Aspergillus parasiticus as affected by various concentrations of glucose and nitrogen in a defined medium and by different incubation temperatures was studied. Maximal yields of lipid and aflatoxin were obtained with 30% glucose, whereas mold growth, expressed as dry weight, was maximal when the medium contained 10% glucose. Maximal mold growth occurred when the medium contained 3% (NH(4))(2)SO(4); however, 1% (NH(4))(2)SO(4) favored maximum accumulation of lipid and aflatoxin. Growth of mold and synthesis of lipid and toxin also varied with the incubation temperature. Maximal mold growth occurred at 35 C, whereas most toxin appeared at 25 C. Maximal production of lipid occurred at 25 and 35 C but production was more rapid at 35 C. Essentially all glucose in the medium (5% initially) was utilized in 3 days at 25 and 35 C but not in 7 days at 15 and 45 C. Patterns for formation of lipid and aflatoxin were similar at 15 and 25 C when a complete growth medium was used and at 28 C when the substrate contained various concentrations of glucose or (NH(4))(2)SO(4). They were dissimilar when the mold grew at 35 or 45 C. At these temperatures lipid was produced preferentially and only small amounts of aflatoxin appeared.
mesh_terms: D000348:Aflatoxins / Q000096:biosynthesis*; D000645:Ammonium Sulfate / Q000378:metabolism*; D001230:Aspergillus / Q000254:growth & development / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D003470:Culture Media; D005947:Glucose / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008055:Lipids / Q000096:biosynthesis*; D013696:Temperature
chemical_list: D000348:Aflatoxins; D003470:Culture Media; D007202:Indicators and Reagents; D008055:Lipids; D005947:Glucose; D000645:Ammonium Sulfate