title: Characterization of anti-Aeromonas salmonicida antibodies from coho salmon.
abstract: Coho salmon (Oncorhynchus kisutch) anti-Aeromonas salmonicida agglutinins were excluded from Sephadex G-200 and their activity was lost after partial reduction and alkylation followed by exposure to 2 M urea. Immunoelectrophoretic analysis of coho antiserum revealed anodic and cathodic populations of immunoglobulin (Ig), and the anti-A. salmonicida antibodies present 12 weeks after parenteral immunization were primarily anodic Ig. Antibodies were specifically purified by dissociation of A. salmonicida cell wall agglutinates with 15% NaCl. The purified Ig was a 17S macroglobulin, and electron microscopy and sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that it was composed of four IgM-like subunits. The minimum coho antibody concentration required for visible agglutination of A. salmonicida cells was approximately 0.006 mug of Ig nitrogen/ml.
mesh_terms: D000333:Aeromonas / Q000276:immunology*; D000372:Agglutination Tests; D000478:Alkylation; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D007122:Immunoelectrophoresis; D007136:Immunoglobulins / Q000032:analysis; D008259:Macroglobulins / Q000032:analysis; D008854:Microscopy, Electron; D008970:Molecular Weight; D010084:Oxidation-Reduction; D011817:Rabbits / Q000276:immunology; D012474:Salmon / Q000276:immunology*; D012965:Sodium Chloride; D014461:Ultracentrifugation; D014508:Urea
chemical_list: D000907:Antibodies, Bacterial; D007136:Immunoglobulins; D008259:Macroglobulins; D012965:Sodium Chloride; D014508:Urea