Isolation and preliminary characterization of the RNA-containing R-type, virus-like particle of BHK-21 cells.
An R-type virus-like particle (VLP) has been isolated from the medium of BHK-21-F cells by ultracentrifugation and polyethylene glycol precipitation. The R-type VLP contains RNA which sediments at 60 to 70S in sucrose density gradients and has a molecular weight of approximately 10(7), as estimated by gel electrophoresis. The R-type VLP can be labeled with (3)H-uridine in the presence of actinomycin D. On the basis of morphology, site of maturation, and preliminary biochemical characterization, the R-type VLP does not appear to fit into any of the major groups of animal viruses.
D000818:Animals; D002250:Carbon Radioisotopes; D002460:Cell Line; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D002999:Clone Cells; D006224:Cricetinae; D003609:Dactinomycin / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D007668:Kidney; D008854:Microscopy, Electron; D012328:RNA Viruses / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D012367:RNA, Viral / Q000302:isolation & purification; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D014779:Virus Replication
D002250:Carbon Radioisotopes; D012367:RNA, Viral; D014316:Tritium; D003609:Dactinomycin; D013936:Thymidine; D014529:Uridine