Production of a potent vaccine from the attenuated M-44 strain of Coxiella burneti.
The attenuated M-44 strain of Coxiella burneti was heated to free it of adventitious agents that may have been present. Parallel titrations of the strain before and after heating indicated that the virulence for guinea pigs was markedly decreased. Guinea pigs immunized with the strain derived after heat treatment were protected from the effects of challenge with C. burneti in either phase I or II.
D000818:Animals; D000917:Antibody Formation; D001428:Bacterial Vaccines; D001831:Body Temperature; D003168:Complement Fixation Tests; D003381:Coxiella / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity; D005069:Evaluation Studies as Topic; D006168:Guinea Pigs; D006358:Hot Temperature; D007114:Immunization; D011778:Q Fever / Q000517:prevention & control*; D014613:Vaccines, Attenuated; D014774:Virulence
D001428:Bacterial Vaccines; D014613:Vaccines, Attenuated