Enzyme measurements on single cells.
A simple and sensitive procedure has been developed for enzymatic assays in single cells and applied to the measurement of beta-glucuronidase in single parenchymal cells of liver. Cells deposited in microdroplets under oil were ruptured by freezing and thawing and allowed to react with a fluorogenic substrate. The fluorescence is developed by diffusing an organic base into the droplets and measured in a fluorescence microscope equipped with a photomultiplier.
D000818:Animals; D002469:Cell Separation; D002477:Cells / Q000201:enzymology*; D003374:Coumarins; D005470:Fluorometry; D005615:Freezing; D005966:Glucuronidase / Q000032:analysis*; D008099:Liver / Q000166:cytology / Q000201:enzymology; D008722:Methods; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D010276:Parasympatholytics
D003374:Coumarins; D010276:Parasympatholytics; D005966:Glucuronidase