Effect of the exotoxin of Bacillus thuringiensis on normal and ecdysone-stimulated ribonucleic acid polymerase activity in intact nuclei from the fat-body of Sarcophaga bullata larvae.
A nuclear preparation from the fat-body of Sarcophaga bullata was obtained which incorporates nucleotides at a steady rate. Two activities, differing in their response to alpha-amanitin, are present. The activities are not separated by changes in the concentration of (NH(4))(2)SO(4) as effectively as in mammalian nuclei. The activity resistant to alpha-amanitin is stimulated by ecdysone, and both normal and the ecdysone-stimulated activities are inhibited by the exotoxin. The amanitin-sensitive enzyme is also inhibited by exotoxin, but higher concentrations are required.
D000273:Adipose Tissue / Q000201:enzymology; D000645:Ammonium Sulfate / Q000494:pharmacology; D000818:Animals; D001407:Bacillus; D001487:Basidiomycota; D002467:Cell Nucleus / Q000201:enzymology*; D002848:Chromatography, DEAE-Cellulose; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004175:Diptera / Q000201:enzymology*; D004440:Ecdysone / Q000037:antagonists & inhibitors / Q000494:pharmacology; D007814:Larva / Q000201:enzymology; D009183:Mycotoxins / Q000494:pharmacology; D009711:Nucleotides / Q000378:metabolism; D009842:Oligopeptides / Q000494:pharmacology; D010456:Peptides, Cyclic / Q000494:pharmacology; D013997:Time Factors; D014118:Toxins, Biological / Q000494:pharmacology*
D009183:Mycotoxins; D009711:Nucleotides; D009842:Oligopeptides; D010456:Peptides, Cyclic; D014118:Toxins, Biological; D004440:Ecdysone; D012321:DNA-Directed RNA Polymerases; D000645:Ammonium Sulfate