Detection of plasma membrane carbohydrates with lectin peroxidase conjugates.
With the use of the cytochemical stain for horseradish peroxidase of Graham and Karnovsky (1966. J. Histochem. Cytochem.14:291), conjugates of horseradish peroxidase with ricin, wheat germ agglutinin, and phytohemagglutinin were employed for the morphologic demonstration of d-galactose (ricin), N-acetylglucosamine (wheat germ), and N-acetylgalactosamine (phytohemagglutinin) containing moieties on the surface of unfixed, or paraformaldehyde-fixed rat lymphoid cells. D-Galactose, or d-galactose containing disaccharides inhibited the interaction between ricin peroxidase and lymphoid cell surface; also, N-acetylglucosamine inhibited the wheat germ peroxidase-lymphoid cell interaction, but N-acetylgalactosamine failed to inhibit the reaction between phytohemagglutinin peroxidase and the surface of lymphoid cells.
D000818:Animals; D001665:Binding Sites; D002241:Carbohydrates / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis* / Q000648:ultrastructure; D002850:Chromatography, Gel; D004187:Disaccharides / Q000032:analysis; D005688:Galactosamine / Q000032:analysis; D005690:Galactose / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D037102:Lectins; D008198:Lymph Nodes / Q000032:analysis; D008214:Lymphocytes / Q000032:analysis; D008854:Microscopy, Electron; D010544:Peroxidases; D037121:Plant Lectins; D010944:Plants / Q000201:enzymology; D011485:Protein Binding; D051381:Rats
D002241:Carbohydrates; D004187:Disaccharides; D037102:Lectins; D037121:Plant Lectins; D005688:Galactosamine; D010544:Peroxidases; D005944:Glucosamine; D005690:Galactose