Scanning density gradient isoelectric focusing of Staphylococcus aureus enterotoxins B and C.
Purified Staphylococcus aureus enterotoxins B and C(1), the causative agents of staphylococcal food poisoning, were resolved into several components by scanning isoelectric focusing in sucrose density gradients in the absence and presence of 6 M urea and 5 mM dithiothreitol. The observed heterogeneity persisted in the denaturing medium, which suggests that the primary structure of protein enterotoxins B and C(1) may be partly responsible for the isoelectric point differences of the various forms. To our knowledge, the scanning isoelectric focusing patterns of staphylococcal enterotoxins B and C(1) are reported for the first time.
D002499:Centrifugation, Density Gradient; D004229:Dithiothreitol / Q000494:pharmacology; D004768:Enterotoxins / Q000032:analysis* / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D013210:Staphylococcus / Q000276:immunology; D013395:Sucrose; D014508:Urea / Q000494:pharmacology
D004768:Enterotoxins; D013395:Sucrose; D014508:Urea; D004229:Dithiothreitol