The reaction of diethyl pyrocarbonate with pyruvate kinase.
Diethyl pyrocarbonate inactivates muscle pyruvate kinase with the substitution of 3-4 histidine residues per subunit. Phosphoenolpyruvate, ATP and ADP to a lesser extent, and Mg(2+) and pyruvate to a small extent, protect against inactivation.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001665:Binding Sites; D002254:Carbonates / Q000494:pharmacology*; D006639:Histidine; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D009132:Muscles / Q000201:enzymology; D010728:Phosphoenolpyruvate / Q000494:pharmacology; D011485:Protein Binding; D011770:Pyruvate Kinase / Q000037:antagonists & inhibitors*; D011773:Pyruvates / Q000494:pharmacology; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
D002254:Carbonates; D011773:Pyruvates; D006639:Histidine; D000244:Adenosine Diphosphate; D010728:Phosphoenolpyruvate; D000255:Adenosine Triphosphate; D011770:Pyruvate Kinase; D008274:Magnesium