title: Isolation of germination mutants of Dictyostelium discoideum.
abstract: A simple method to separate spores from amoebae of Dictyostelium discoideum has been devized and used to isolate spore germination mutants. A subclass of these mutants is temperature sensitive for germination and growth.
mesh_terms: D002498:Centrifugation; D004754:Enterobacter / Q000254:growth & development; D005658:Fungi / Q000254:growth & development* / Q000302:isolation & purification; D008722:Methods; D009153:Mutagens; D009154:Mutation; D009604:Nitrosoguanidines; D011134:Polysaccharides; D013172:Spores, Fungal / Q000254:growth & development*; D013696:Temperature
chemical_list: D009153:Mutagens; D009604:Nitrosoguanidines; D011134:Polysaccharides