Determination of clofibrate in biological fluids by thin-layer and gas-liquid chromatography.
A specific and sensitive method is described for the detection of clofibrate in biological fluids. The drug is separated from associated fatty acids by thin-layer chromatography and the methyl ester is quantified by gas-liquid chromatography. Recovery is excellent, and any small losses are corrected with an internal recovery standard. Although more time-consuming than other available techniques, the method offers advantages for accurate studies of clofibrate metabolism.
D001647:Bile Acids and Salts / Q000032:analysis; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D002994:Clofibrate / Q000032:analysis* / Q000097:blood / Q000652:urine; D005243:Feces / Q000032:analysis; D006801:Humans; D008722:Methods; D014316:Tritium
D001647:Bile Acids and Salts; D014316:Tritium; D002994:Clofibrate