Latex agglutination test for detection of Australia antigen (HB-Ag) among blood donors and patients.
The application of the latex test for the detection of Australia antigen (Au-Ag) was investigated. Reagents from two commercial sources were compared with the electrophoresis method with regard to sensitivity and specificity using samples from blood donors, hospital patients, and plasma fractions. Discordant results were further investigated by electron microscopy and radioimmunoassay. Differences were noted in the results between these reagents and the significance of the findings together with suggestions for minimizing false positive results are discussed.
D000328:Adult; D000372:Agglutination Tests; D000818:Animals; D001172:Arthritis, Rheumatoid / Q000276:immunology; D001782:Blood Donors; D005188:False Negative Reactions; D005189:False Positive Reactions; D006168:Guinea Pigs / Q000276:immunology; D006511:Hepatitis B Antigens / Q000032:analysis*; D006358:Hot Temperature; D006801:Humans; D007122:Immunoelectrophoresis; D007136:Immunoglobulins / Q000032:analysis; D007457:Iodine Radioisotopes; D007841:Latex Fixation Tests; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D009995:Osmosis; D011233:Precipitin Tests; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay
D006511:Hepatitis B Antigens; D007136:Immunoglobulins; D007457:Iodine Radioisotopes