Intracellular viral RNA species in mouse cells nonproductively transformed by the murine sarcoma virus.
The size and quantity of virus-specific RNA in five non-virus-producing mouse cells transformed by the Moloney isolate of murine sarcoma virus (MSV) was determined. Hybridization of RNA from transformed cells with the [(3)H]DNA product of the RNA-directed DNA polymerase of the murine sarcoma-leukemia virus was used to detect and quantitate virus-specific RNA. The amount of virus-specific RNA in non-virus-producing cells was less than one-sixth of that found in virus-producing cells. A striking correlation was found between the amount of intracellular virus-specific RNA and the degree of agglutination by conconavalin A previously reported for the four non-virus-producing NIH/3T3 cell lines (Salzberg and Green, 1974). A major RNA subunit sedimenting at 26 to 28S was detected in all five MSV-transformed non-virus-producing cells. This could represent the RNA genome of defective MSV.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002501:Centrifugation, Zonal; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts; D066298:In Vitro Techniques; D051379:Mice; D008815:Mice, Inbred Strains / Q000196:embryology; D008970:Molecular Weight; D008979:Moloney murine leukemia virus; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains / Q000196:embryology; D014316:Tritium
D012367:RNA, Viral; D014316:Tritium