Enrichment of mutants lacking the phosphoenolpyruvate-dependent phosphotransferase system of Vibrio parahaemolyticus by screening with methyl-alpha-D-glucoside.
Screening with methyl-alpha-d-glucoside was an efficient procedure for enrichment of mutants lacking the glucose transport system and of the pleiotropic mutants lacking the phosphoenolpyruvate-dependent phosphotransferase system in Vibrio parahaemolyticus.
D002250:Carbon Radioisotopes; D004352:Drug Resistance, Microbial; D005947:Glucose / Q000378:metabolism*; D008759:Methylglycosides / Q000378:metabolism* / Q000494:pharmacology; D009154:Mutation; D010728:Phosphoenolpyruvate; D010770:Phosphotransferases / Q000096:biosynthesis*; D014733:Vibrio / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism*
D002250:Carbon Radioisotopes; D008759:Methylglycosides; D010728:Phosphoenolpyruvate; D010770:Phosphotransferases; D005947:Glucose