The use of radiolabelled T1 ribonuclease to monitor the efficiency of Edman degradations.
A simple radiolabel tracer method is presented for monitoring the efficiency of the first ten cycles of an Edman degradation. This method consists of adding a very small amount of radiolabelled S-carboxymethylated T(1) ribonuclease to the protein to be sequenced, and counting the radioactivity of a small sample from each of the degradative cycles.
D000596:Amino Acids / Q000032:analysis; D002850:Chromatography, Gel; D007461:Iodoacetates; D008722:Methods; D008745:Methylation; D009211:Myoglobin / Q000032:analysis; D012260:Ribonucleases / Q000032:analysis*; D014316:Tritium
D000596:Amino Acids; D007461:Iodoacetates; D009211:Myoglobin; D014316:Tritium; D012260:Ribonucleases