A lipid-soluble antioxidant from polyallomer centrifuge tubes.
Santowhite, a commercial antioxidant used in the manufacture of polypropylene, contaminates 12-ml polyallomer tubes to the extent of 0.2-0.3 mg/tube. It is distributed through the plastic and appears as a microscopic dust on the tubes' surfaces. In the preparation of polysomes from rat liver by standard methods, approximately 15% of the antioxidant in previously water-washed tubes was removed by suspension of the polysome pellet with stirring in 1-2 ml of Tris buffer, pH 7.8. The polyallomer impurity has been shown to be identical with Santowhite, which is 4,4'-butylidene-bis(6-tert-butyl-m-cresol), by UV, mass, and NMR spectra. It is not uniformly removed from polyallomer tubes by common detergents but is removed by washing with acetone, to which the plastic is resistant.
D000096:Acetone; D000818:Animals; D000975:Antioxidants; D003902:Detergents; D005069:Evaluation Studies as Topic; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000032:analysis*; D008099:Liver / Q000166:cytology; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008722:Methods; D010969:Plastics; D011108:Polymers; D011132:Polyribosomes; D051381:Rats; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet; D014461:Ultracentrifugation; D014867:Water
D000975:Antioxidants; D003902:Detergents; D008055:Lipids; D010969:Plastics; D011108:Polymers; D014867:Water; D000096:Acetone