A comparison of media used in vitro to isolate non-sporing Gram-negative anaerobes from blood.
Five anaerobic media were compared in a model blood culture system for their ability to recover small inocula of Gram-negative non-sporing anaerobes. Dehydrated cooked meat medium was the least effective; USP thioglycollate medium was the most effective isolation medium and is recommended for routine use. Freshly prepared cooked meat medium has the advantage of allowing prolonged survival of strains.
D000693:Anaerobiosis; D001419:Bacteria / Q000302:isolation & purification*; D001439:Bacteroides / Q000302:isolation & purification; D001769:Blood / Q000382:microbiology*; D003470:Culture Media; D008460:Meat; D008722:Methods; D013864:Thioglycolates
D003470:Culture Media; D013864:Thioglycolates