Biochemical and ultrastructural aspects of Ca2+ transport by mitochondria of the hepatopancreas of the blue crab Callinectes sapidus.
Mitochondria isolated from the hepatopancreas of the blue crab Callinectes sapidus show up to 12-fold stimulation of respiration on addition of Ca(2+), which is accompanied by Ca(2+) accumulation (Ca(2+):site = 1.9) and H(+) ejection (H(+):Ca(2+) = 0.85). Sr(2+) and Mn(2+) are also accumulated; Mg(2+) is not. A strongly hypertonic medium (383 mosM), Mg(2+), and phosphate are required for maximal Ca(2+) uptake. Ca(2+) uptake takes precedence over oxidative phosphorylation of ADP for respiratory energy. Once Ca(2+) is accumulated by the crab mitochondria, it is stable and only very slowly released, even by uncoupling agents. ATP hydrolysis also supports Ca(2+) uptake. Respiration-inhibited crab hepatopancreas mitochondria show both high-affinity and low-affinity Ca(2+)-binding sites, which are inactive in the presence of uncoupling agents. Crab hepatopancreas mitochondria have an enormous capacity for accumulation of Ca(2+), up to 5,500 ng-atoms Ca(2+) per mg protein, with an equivalent amount of phosphate. Freshly isolated mitochondria contain very large amounts of Ca(2+), Mg(2+), phosphate, K(+), and Na(+); their high Ca(2+) content is a reflection of the vary large amount of extra-mitochondrial Ca(2+) in the whole tissue. Electron microscopy of crab mitochondria loaded with Ca(2+) and phosphate showed large electron-dense deposits, presumably of precipitated calcium phosphate. They consisted of bundles of needle-like crystals, whereas Ca(2+)-loaded rat liver mitochondria show only amorphous deposits of calcium phosphate under similar conditions. The very pronounced capacity of crab hepatopancreas mitochondria for transport of Ca(2+) appears to be adapted to a role in the storage and release of Ca(2+) during the molting cycle of this crustacean.
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D003386:Brachyura / Q000166:cytology / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002130:Calcium Phosphates / Q000096:biosynthesis; D004579:Electron Transport; D006859:Hydrogen / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism; D008854:Microscopy, Electron; D008928:Mitochondria / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000166:cytology / Q000378:metabolism; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D010179:Pancreas / Q000166:cytology / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D014475:Uncoupling Agents / Q000494:pharmacology
D002130:Calcium Phosphates; D010710:Phosphates; D014475:Uncoupling Agents; D000244:Adenosine Diphosphate; D000968:Antimycin A; D006859:Hydrogen; D000255:Adenosine Triphosphate; D008274:Magnesium; D002118:Calcium