Purification and assays for the presence and biological functions of two eukaryotic initiation factors from rabbit reticulocytes that are required for initiation complex formation with rabbit reticulocyte ribosomes.
D000818:Animals; D001665:Binding Sites; D001798:Blood Proteins / Q000096:biosynthesis; D002499:Centrifugation, Density Gradient; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D005069:Evaluation Studies as Topic; D006160:Guanosine Triphosphate; D008099:Liver / Q000378:metabolism; D008715:Methionine; D008722:Methods; D008970:Molecular Weight; D010442:Peptide Chain Initiation, Translational; D010448:Peptide Initiation Factors / Q000032:analysis*; D011132:Polyribosomes / Q000187:drug effects / Q000378:metabolism; D011485:Protein Binding; D014176:Protein Biosynthesis; D011691:Puromycin / Q000494:pharmacology; D012343:RNA, Transfer / Q000378:metabolism; D011817:Rabbits; D011955:Receptors, Drug; D012156:Reticulocytes / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013462:Sulfur Radioisotopes; D014316:Tritium
D001798:Blood Proteins; D010448:Peptide Initiation Factors; D011955:Receptors, Drug; D013462:Sulfur Radioisotopes; D014316:Tritium; D011691:Puromycin; D006160:Guanosine Triphosphate; D012343:RNA, Transfer; D008715:Methionine