Affinity labelling of isoelectrofocused fractions from a DNP antibody preparation with the photoactive labels 2,4-dinitrophenyl-1-azide and 2,4-dinitrophenyl-epsilon-aminocaproyldiazoketone.
D000478:Alkylation; D000614:Aminocaproates; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D001386:Azides; D001666:Binding Sites, Antibody; D002845:Chromatography; D004140:Dinitrophenols; D004229:Dithiothreitol; D007128:Immunoglobulin Fragments; D007525:Isoelectric Focusing; D007659:Ketones; D013058:Mass Spectrometry; D010084:Oxidation-Reduction; D010777:Photochemistry; D011817:Rabbits / Q000276:immunology; D013381:Subtilisins; D014316:Tritium
D000614:Aminocaproates; D000906:Antibodies; D001386:Azides; D004140:Dinitrophenols; D007128:Immunoglobulin Fragments; D007659:Ketones; D014316:Tritium; D013381:Subtilisins; D004229:Dithiothreitol