A simple and inexpensive membrane "lung" for small organ perfusion.
A disposable coil of thin-walled Silastic tubing, permeable to oxygen and carbon dioxide, functioned as a "lung" for perfusion of isolated rat liver. This "lung," which can be easily assembled from laboratory supplies, has a number of advantages over devices that utilize large air-liquid interfaces for gas exchange. Rates of hepatic lipoprotein triglyceride secretion, comparable to those obtained with more complex oxygenation systems, are achieved with this simple device.
D000818:Animals; D002245:Carbon Dioxide; D003365:Costs and Cost Analysis; D004912:Erythrocytes / Q000378:metabolism; D005069:Evaluation Studies as Topic; D006400:Hematocrit; D008074:Lipoproteins / Q000097:blood; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008722:Methods; D008854:Microscopy, Electron; D010100:Oxygen; D010101:Oxygen Consumption; D010477:Perfusion / Q000295:instrumentation*; D051381:Rats; D014280:Triglycerides / Q000378:metabolism
D008074:Lipoproteins; D014280:Triglycerides; D002245:Carbon Dioxide; D010100:Oxygen