Peptidoglycan biosynthesis by preparations from Bacillus licheniformis: cross-linking of newly synthesized chains to preformed cell wall.
A wall-plus-membrane preparation from a Bacillus licheniformis mutant incorporated radioactivity from a peptidoglycan precursor in which the free amino group of diaminopimelic acid was blocked by (14)C-labelled acetyl group. This incorporation was penicillin-sensitive. The enzymically degraded product contained cross-linked dimers, showing that newly synthesized peptidoglycan chains had been cross-linked to the pre-existing cell wall.
D000599:Amino Acids, Diamino / Q000378:metabolism; D001407:Bacillus / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002462:Cell Membrane; D002473:Cell Wall / Q000378:metabolism*; D002850:Chromatography, Gel; D004586:Electrophoresis; D009154:Mutation; D010406:Penicillins / Q000378:metabolism; D010457:Peptidoglycan / Q000096:biosynthesis*; D010867:Pimelic Acids / Q000378:metabolism
D000599:Amino Acids, Diamino; D002250:Carbon Radioisotopes; D010406:Penicillins; D010457:Peptidoglycan; D010867:Pimelic Acids