Quantitative analysis of cholesterol in 5 to 20 microliter of plasma.
A gas-liquid chromatographic micromethod for quantitation of cholesterol in 20 micro l of plasma was developed using 5alpha-cholestane as an internal standard, saponification with tetramethylammonium hydroxide-isopropanol, and extraction with tetrachloroethylene-methyl butyrate. Cholesterol levels in plasma samples were calculated by comparing cholesterol-cholestane peak height ratios with those of preassayed reference plasma. Over a plasma cholesterol range of 44 to 468 mg/100 ml, the gas-liquid chromatographic micromethod and the automated ferric chloride colorimetric method gave nearly identical results (r = 0.99) in duplicate aliquots of 131 plasma samples.
D000328:Adult; D000704:Analysis of Variance; D001322:Autoanalysis; D001769:Blood; D002648:Child; D002784:Cholesterol / Q000097:blood*; D002849:Chromatography, Gas; D003124:Colorimetry; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D008433:Mathematics; D008722:Methods; D008832:Microchemistry; D011247:Pregnancy; D013997:Time Factors; D014470:Umbilical Cord
D007202:Indicators and Reagents; D002784:Cholesterol