A technique for isolating pure, viable parenchymal cells and their nuclei from rat mammary gland.
D000818:Animals; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000032:analysis*; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D003600:Cytosol / Q000032:analysis; D004247:DNA / Q000032:analysis; D005260:Female; D008239:Lysine / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000032:analysis / Q000166:cytology*; D008722:Methods; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis; D012313:RNA / Q000032:analysis; D051381:Rats; D013997:Time Factors; D014316:Tritium; D014357:Trypsin
D011506:Proteins; D014316:Tritium; D012313:RNA; D004247:DNA; D014357:Trypsin; D008239:Lysine