title: Purification of ficin by affinity chromatography.
mesh_terms: D002846:Chromatography, Affinity; D004589:Electrophoresis, Disc; D010450:Endopeptidases / Q000302:isolation & purification* / Q000378:metabolism; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D008628:Mercury; D008722:Methods; D010206:Papain / Q000302:isolation & purification; D011134:Polysaccharides; D013056:Spectrophotometry, Ultraviolet; D013438:Sulfhydryl Compounds
chemical_list: D011134:Polysaccharides; D013438:Sulfhydryl Compounds; D010450:Endopeptidases; D010206:Papain; D008628:Mercury