Purification and properties of undecyl acetate esterase from Pseudomonas cepacia grown on 2-tridecanone.
Undecyl acetate esterase has been purified from Pseudomonas cepacia grown on the methyl ketone, 2-tridecanone. The K(m) for undecyl acetate was 2.3 x 10(-2) M. Polyacrylamide gel electrophoresis indicated that two esterase bands were being recovered during purification. These bands were separated by preparative polyacrylamide gel electrophoresis. Molecular weights were estimated to be approximately 34,500 by several methods. Molecular sieve polyacrylamide gel electrophoresis indicated that the two esterases had the same molecular weight but different charge, which is indicative of isoenzymes.
D000085:Acetates / Q000378:metabolism; D000645:Ammonium Sulfate; D002250:Carbon Radioisotopes; D011232:Chemical Precipitation; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D003124:Colorimetry; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D004950:Esterases / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007659:Ketones / Q000378:metabolism*; D008365:Manometry; D008970:Molecular Weight; D011549:Pseudomonas / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D013696:Temperature; D014316:Tritium
D000085:Acetates; D002250:Carbon Radioisotopes; D003470:Culture Media; D007659:Ketones; D014316:Tritium; D004950:Esterases; D000645:Ammonium Sulfate