Temperature-sensitive virus from Aedes albopictus cells chronically infected with Sindbis virus.
Cultures of Aedes albopictus cells persistently infected with wild-type Sindbis virus (SV-W) give rise to small plaque-forming mutants which are also temperature sensitive. These mutants, designated SV-C, are neutralized by antiserum produced against SV-W. Mutant ts clones were isolated from SV-C by plaque purification. After serial undiluted passage in BHK or mosquito cells, each of the clones gave rise to ts(+) revertants which, however, remained mutant with respect to plaque morphology. Nineteen of 20 clones derived from SV-C were RNA(+), and one was RNA(-) (SV-C-2). The RNA synthesizing activity, once induced in infected cells by SV-C-2, was stable at the nonpermissive temperature (39.5 C). All clones derived from SV-C were inactivated at 60 C much more quickly than was SV-W. It was not possible to demonstrate complementation between any of the SV-C clones.
D000330:Aedes; D000818:Animals; D000956:Antigens, Viral; D002460:Cell Line; D002642:Chick Embryo; D006224:Cricetinae; D046508:Culture Techniques; D003609:Dactinomycin / Q000494:pharmacology; D005816:Genetic Complementation Test; D014644:Genetic Variation; D006386:Hemagglutination Tests; D007668:Kidney; D009154:Mutation; D009500:Neutralization Tests; D010641:Phenotype; D012367:RNA, Viral / Q000096:biosynthesis; D012845:Sindbis Virus / Q000254:growth & development* / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D013696:Temperature; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D010948:Viral Plaque Assay; D014776:Virus Cultivation
D000956:Antigens, Viral; D012367:RNA, Viral; D014316:Tritium; D003609:Dactinomycin; D014529:Uridine