Iron and aconitase activity.
Aconitase activated with Fe(2+), cysteine and ascorbate incorporates 1 g-atom of Fe(2+)/mol. Loss of this Fe(2+) by transfer to ferrozine, a Fe(2+) chelator, results in loss of activity. Ascorbate increases the rate of transfer of the essential Fe(2+) whereas citrate retards the rate of transfer. Transfer of Fe(2+) from inactive aconitase, 2 g-atoms of Fe/mol, can be accomplished in the presence of urea and ascorbate. The correlation of activity with the presence of an added g-atom of Fe(2+)/mol leads to the conclusion that active aconitase has only one active site per mol.
D000154:Aconitate Hydratase / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism; D001557:Benzenesulfonates; D002614:Chelating Agents; D002850:Chromatography, Gel; D002951:Citrates / Q000378:metabolism; D003545:Cysteine / Q000378:metabolism; D006836:Hydro-Lyases / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D011725:Pyridines; D013053:Spectrophotometry; D013696:Temperature; D013997:Time Factors; D014227:Triazines; D014508:Urea / Q000378:metabolism
D001557:Benzenesulfonates; D002614:Chelating Agents; D002951:Citrates; D011725:Pyridines; D014227:Triazines; D014508:Urea; D007501:Iron; D006836:Hydro-Lyases; D000154:Aconitate Hydratase; D003545:Cysteine; D001205:Ascorbic Acid