Halophilic nuclease from a moderately halophilic Micrococcus varians.
The moderately halophilic bacterium Micrococcus varians, isolated from soy sauce mash, produced extracellular nuclease when cultivated aerobically in media containing 1 to 4 M NaCl or KCl. The enzyme, purified to an electrophoretically homogeneous state, had both ribonuclease and deoxyribonuclease activities. The nuclease had maximal activity in the presence of 2.9 M NaCl or 2.1 M KCl at 40 C. The enzymatic activity was lost by dialysis against low-salt buffer, whereas when the inactivated enzyme was dialyzed against 3.4 M NaCl buffer as much as 77% of the initial activity could be restored.
D002474:Cell-Free System; D003956:Dialysis; D004589:Electrophoresis, Disc; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000494:pharmacology; D008836:Micrococcal Nuclease / Q000302:isolation & purification / Q000378:metabolism*; D008837:Micrococcus / Q000201:enzymology*; D011189:Potassium Chloride / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology
D008670:Metals; D012965:Sodium Chloride; D011189:Potassium Chloride; D008836:Micrococcal Nuclease