Humoral immunostimulation. I. Increased uptake of (125I)iododeoxyuridine and (3H)thymidine into TNP-cells treated with anti-TNP antibody.
Interaction of microgram quantities of highly purified rabbit anti-TNP antibodies with TNP-substituted HeLa, HEp-2, and L cells caused an intense stimulation of radioactive nucleoside ([(125)I]UdR and [(3)H]TdR) uptake which was maximal 24-72 h after exposure of cells to antibody. The stimulation of nucleoside uptake and presumaly DNA synthesis was shown to be immuno logically mediated because unsubstituted cells were not stimulated by anti-TNP antibody, normal rabbit gamma globulin did not stimulate TNP-cells, and a hapten inhibitor, epsilon-DNP-lysine, prevented the stimulation of TNP-cells by anti-TNP antibody. These findings demonstrate that interaction of antibody with cell surface antigen can alter cell membrane transport, and possibly can enhance cell growth.
D000818:Animals; D000906:Antibodies; D002277:Carcinoma; D002460:Cell Line; D005260:Female; D005949:Glucose Oxidase; D006367:HeLa Cells; D006801:Humans; D007065:Idoxuridine / Q000378:metabolism*; D007454:Iodides; D007457:Iodine Radioisotopes; D007700:Kinetics; D007739:L Cells; D007822:Laryngeal Neoplasms; D051379:Mice; D009369:Neoplasms / Q000276:immunology* / Q000378:metabolism; D009596:Nitrophenols; D010544:Peroxidases; D013936:Thymidine / Q000378:metabolism*; D014316:Tritium; D014357:Trypsin / Q000494:pharmacology
D000906:Antibodies; D007454:Iodides; D007457:Iodine Radioisotopes; D009596:Nitrophenols; D014316:Tritium; D005949:Glucose Oxidase; D010544:Peroxidases; D014357:Trypsin; D007065:Idoxuridine; D013936:Thymidine