title: Use of Japanese quail embryo fibroblast cells for propagation and assay of turkey herpesvirus FC-126.
abstract: Japanese quail (Coturnix coturnix japonica) fibroblast cell culture monolayers were found to provide a very satisfactory system in which to propagate and assay turkey herpesvirus FC-126, which is used for production of Marek's disease vaccine. Japanese quail cells were more sensitive than duck cells and of approximately equal sensitivity to chicken cells. Foci of infection developed rapidly and uniformly, were of larger size, and were more easily discernible in quail cells than in chicken cells.
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000382:microbiology; D002478:Cells, Cultured; D002642:Chick Embryo; D003370:Coturnix; D046508:Culture Techniques; D003588:Cytopathogenic Effect, Viral; D003593:Cytoplasm / Q000382:microbiology; D004372:Ducks; D005347:Fibroblasts; D006564:Herpesviridae / Q000254:growth & development* / Q000302:isolation & purification; D007181:Inclusion Bodies, Viral; D008380:Marek Disease / Q000382:microbiology*; D008854:Microscopy, Electron; D014765:Viral Vaccines; D014776:Virus Cultivation
chemical_list: D014765:Viral Vaccines