Thermal inactivation as a means of inhibiting the serum-associated deamination of 9-beta-D Arabinofuranosyladenine in tissue culture media.
9-beta-d-Arabinofuranosyladenine (ara-A) was deaminated to 9-beta-d-arabinofuranosylhypoxanthine by adenosine deaminase present in fetal bovine serum, newborn calf serum, and calf serum used to supplement tissue culture media. Heating newborn calf serum or calf serum for 12 h at 56 C completely eliminated the enzymatic deamination of ara-A. The deaminase activity associated with fetal bovine serum was more refractory to heating, requiring 24 h for complete inactivation. The nutritive value of heat-inactivated calf serum did not differ significantly from that of unheated serum based on considerations of population doubling times, deoxyribonucleic acid synthesis, and relative cloning efficiencies of KB cells.
D000225:Adenine / Q000378:metabolism; D000619:Aminohydrolases / Q000378:metabolism; D001089:Arabinose / Q000378:metabolism; D002277:Carcinoma; D002460:Cell Line; D002478:Cells, Cultured; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D003641:Deamination; D006358:Hot Temperature; D009062:Mouth Neoplasms; D009705:Nucleosides / Q000378:metabolism*
D003470:Culture Media; D009705:Nucleosides; D004247:DNA; D001089:Arabinose; D000619:Aminohydrolases; D000225:Adenine