title: Occurrence of a complex form of staphylokinase in the course of cultivation of Staphylococcus aureus.
abstract: Proteins in the culture supernatant of Staphylococcus aureus PS 47 were subjected to Sephadex chromatography. In the early stage of the cultivation, staphylokinase appeared to have a molecular weight of 15,000 and in the later stage it appeared to have a molecular weight of 320,000. The staphylokinase having a lower molecular weight (type A) converted into one having a higher value (type B) during the course of cultivation. It was demonstrated that conversion of type A into type B took place in vitro (monitored by gel filtration after the two types of staphylokinases were mixed), and it was observed that type B reverted to type A when type B was treated with KCl or detergent. Type B seems to be a complex form of type A and some high-molecular-weight substance.
mesh_terms: D000942:Antigens, Bacterial; D002850:Chromatography, Gel; D010450:Endopeptidases / Q000096:biosynthesis* / Q000378:metabolism; D006358:Hot Temperature; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000096:biosynthesis* / Q000378:metabolism; D009500:Neutralization Tests; D011189:Potassium Chloride / Q000494:pharmacology; D013210:Staphylococcus / Q000201:enzymology* / Q000276:immunology; D013501:Surface-Active Agents / Q000494:pharmacology; D013997:Time Factors
chemical_list: D000942:Antigens, Bacterial; D009097:Multienzyme Complexes; D013501:Surface-Active Agents; D011189:Potassium Chloride; D010450:Endopeptidases