Limiting nutrients during mating as a means of increasing recombinant recovery in crosses of Nocardia erythropolis.
Minimal medium supplemented with sub-optimal levels of parental auxotrophic requirements increases recombinant recovery from matings of Nocardia erythropolis more than 500-fold when compared with nutrient agar matings.
D001976:Bromouracil / Q000494:pharmacology; D002470:Cell Survival; D003227:Conjugation, Genetic; D003433:Crosses, Genetic; D003470:Culture Media; D009154:Mutation; D009615:Nocardia / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D011830:Radiation Effects; D011995:Recombination, Genetic; D014466:Ultraviolet Rays
D003470:Culture Media; D001976:Bromouracil