title: Assembly of chick brain tubulin onto isolated basal bodies of Chlamydomonas reinhardi.
abstract: Basal bodies isolated from Chlamydomonas reinhardi will serve as initiation centers for the assembly of chick brain microtubule protein subunits (tubulin) into microtubules. The rate of microtubule assembly is tubulin-concentration dependent; this assembly occurs onto both distal and proximal ends of the basal body mnicrotubules, with distal assembly greatly favored. In vitro assembly of brain tubulin also occurs onto the mid-lateral aspects of the basal bodies, presumably onto the fiber connecting the two basal bodies.
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology*; D001923:Brain Chemistry; D002458:Cell Fractionation; D002696:Chlamydomonas / Q000166:cytology / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005407:Flagella; D008870:Microtubules / Q000032:analysis; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000302:isolation & purification
chemical_list: D009419:Nerve Tissue Proteins