title: Structural aberrations in T-even bacteriophage. V. Effects of canavanine on the maturation and utilization of specific gene products.
abstract: Previous results have shown that when a T-even bacteriophage-infected cell was exposed to l-canavanine followed by an exposure to l-arginine, a monster phage particle, termed a lollipop, was formed. l-Canavanine was necessary for the induction event but l-arginine was required for the maturation of the particle. We now describe the effects of canavanine on the maturation of certain T4 proteins and their role in the induction of lollipops. The cleavage reactions of the head proteins P22, P23, P24, and IPIII are prevented by l-canavanine as shown by the accumulation of the precursor proteins and the failure of the cleaved products to appear. l-Canavanine also prevents the appearance of P12 (tailplate protein) and P20 (head protein) indicating that these proteins may undergo a proteolytic cleavage during normal assembly. The formation of P10 (tailplate protein) and P18 (tail sheath protein) is also affected by l-canavanine. The data suggest that P23 in conjunction with P20 plays a major role in the determination of the length of the phage head.
mesh_terms: D001345:Autoradiography; D002172:Canavanine / Q000494:pharmacology*; D002250:Carbon Radioisotopes; D002501:Centrifugation, Zonal; D003090:Coliphages / Q000187:drug effects / Q000254:growth & development*; D004267:DNA Viruses / Q000187:drug effects / Q000254:growth & development; D004591:Electrophoresis, Polyacrylamide Gel; D007930:Leucine; D008854:Microscopy, Electron; D010649:Phenylalanine / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects*; D012967:Sodium Dodecyl Sulfate; D014764:Viral Proteins
chemical_list: D002250:Carbon Radioisotopes; D014764:Viral Proteins; D012967:Sodium Dodecyl Sulfate; D002172:Canavanine; D010649:Phenylalanine; D007930:Leucine