Chemical and physical properties of lipopolysaccharide of Yersinia pestis.
Lipopolysaccharide (LPS) prepared from Yersinia pestis 195/P contained d-glucose, d-glycero-d-mannoheptose, l-glycero-d-mannoheptose, glucosamine, 3-deoxyoctulosonic acid, lipid A, beta-hydroxymyristate, acetyl, phosphate, and protein. Traces of ethanolamine, mannose, and galactose were also detected. The lipid A moiety was composed of glucosamine substituted with phosphate, amide-linked beta-hydroxymyristate, and amide-bound acetate. The absence of significant amounts of additional fatty acids indicates a lipid A structure somewhat less complex than that of other gram-negative bacteria. The sugars identified are those generally found in the "core" region of LPS from the Enterobacteriaceae, with the exception of the d-glycero-d-mannoheptose. The molecular weight of the aggregated LPS was estimated to be 1.6 x 10(8).
D000596:Amino Acids / Q000032:analysis; D001426:Bacterial Proteins / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D005227:Fatty Acids / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006539:Heptoses / Q000032:analysis; D006595:Hexosamines / Q000032:analysis; D008055:Lipids / Q000032:analysis; D008070:Lipopolysaccharides / Q000032:analysis*; D008970:Molecular Weight; D010758:Phosphorus / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D013055:Spectrophotometry, Infrared; D014461:Ultracentrifugation; D014783:Viscosity; D015010:Yersinia pestis / Q000032:analysis*
D000596:Amino Acids; D001426:Bacterial Proteins; D002241:Carbohydrates; D005227:Fatty Acids; D006539:Heptoses; D006595:Hexosamines; D008055:Lipids; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D010758:Phosphorus; D005947:Glucose