Potentiation of the antibacterial effect of methenamine by acetohydroxamic acid.
In vitro testing shows nearly all strains of Proteus to be susceptible to methenamine. However, infection by urease-producing bacteria alkalinizes the urine in vivo and prevents generation of formaldehyde, the active metabolite, from methenamine. We have previously shown acetohydroxamic acid (AHA) to be an effective inhibitor of bacterial urease in vitro and in vivo. We now present data obtained by use of static and dynamic in vitro systems, which show that, by preventing urease-induced alkalinization of urine, AHA enables methenamine to exert its antibacterial effect against representative Proteus species.
D004357:Drug Synergism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006877:Hydroxamic Acids / Q000494:pharmacology*; D008709:Methenamine / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D011511:Proteus / Q000187:drug effects*; D014510:Urease / Q000037:antagonists & inhibitors; D014556:Urine / Q000382:microbiology
D006877:Hydroxamic Acids; D014510:Urease; D008709:Methenamine