title: Purification on renografin density gradients of Chlamydia trachomatis grown in the yolk sac of eggs.
abstract: Chlamydia trachomatis grown in the yolk sac of embryonated eggs was purified by centrifugation on continuous isopycnic Renografin density gradients. A band of chlamydial particles with a buoyant density of 1.20 contained 70% of the starting particles, and electron microscopy revealed the virtual absence of contaminating egg material. Centrifugation on Renografin gradients caused only a moderate decrease in infectivity. For large-scale purification, infected yolk sac was centrifuged through Renografin solutions, resulting in greater than 60% recovery of starting chlamydial particles, but less than 1% recovery of the dry weight and protein.
mesh_terms: D000818:Animals; D002452:Cell Count; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D002689:Chlamydia / Q000254:growth & development / Q000302:isolation & purification* / Q000472:pathogenicity; D003973:Diatrizoate; D005260:Female; D008722:Methods; D008854:Microscopy, Electron; D014817:Vitelline Membrane / Q000382:microbiology
chemical_list: D003973:Diatrizoate