Base composition and hybridization studies of the three double-stranded RNA segments of bacteriophage phi 6.
The three double-stranded ribonucleic acid (dsRNA) segments of the bacteriophage phi6 were isolated and shown to have similar melting temperatures and base compositions. RNA:RNA hybridization experiments with the isolated segments eliminate the possibility that the two smaller dsRNA segments arise from a cleavage of the large dsRNA segment. The two smaller RNA segments reanneal rapidly even at low temperatures; in contrast, the large dsRNA reannealed only at higher temperatures. Evidence is also presented which suggests that the dsRNAs may contain a short single-stranded RNA tail.
D001435:Bacteriophages / Q000032:analysis*; D002501:Centrifugation, Zonal; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009711:Nucleotides / Q000032:analysis*; D010761:Phosphorus Radioisotopes; D011549:Pseudomonas; D012367:RNA, Viral / Q000302:isolation & purification*; D012260:Ribonucleases
D009711:Nucleotides; D010761:Phosphorus Radioisotopes; D012367:RNA, Viral; D012260:Ribonucleases