Characterization of Bacillus licheniformis 6346 mutants which have altered lytic enzyme activities.
Two groups of mutants altered in lytic enzyme activities have been isolated from Bacillus licheniformis 6346 MH-1 by screening clones for halo production in agar plates containing cell wall conjugated with Procion brilliant red. In the first group which produced halos during colony formation, two were shown to contain three- and eightfold more muramyl-l-alanine amidase than the parent. These strains liberated amidase and intracellular alpha-glucosidase into the culture medium during exponential growth in liquid medium. Isolated walls had a normal qualitative composition and in autolysing liberated N-terminal amino acids and reducing groups. Wall preparations from the second group of mutants which did not produce halos lysed very poorly at pH 9.5, the optimal pH for amidase activity, and poorly at pH 5.5 even though they had similar endo-N-acetylglucosaminidase activities to the parent. Two of these strains that were also deficient in phosphoglucomutase had only 3 to 5% of the membrane-bound amidase activity compared with that in the parent. Cell walls of the phosphoglucomutase-deficient mutants treated with sodium dodecyl sulfate to inactivate endogenous lytic enzymes were dissolved at 10% of the rate of those from the parent by added amidase, but their sensitivities to lysozyme were similar. Those from one mutant had 10 to 20% of the amidase-binding capacity of parent walls, whereas its isolated mucopeptide was essentially inactive in this respect. The failure of these phosphoglucomutase-deficient mutants to autolyse is likely to be due to the combined effects of both low amidase activity and resistant walls. As a result, daughter cells are unable to separate and long chains are formed during exponential growth.
D000581:Amidohydrolases / Q000378:metabolism*; D000596:Amino Acids / Q000032:analysis; D000606:Amino Sugars / Q000032:analysis; D001407:Bacillus / Q000032:analysis / Q000201:enzymology* / Q000254:growth & development; D001433:Bacteriolysis; D002241:Carbohydrates / Q000032:analysis; D002462:Cell Membrane / Q000201:enzymology; D002473:Cell Wall / Q000032:analysis / Q000201:enzymology / Q000378:metabolism; D003593:Cytoplasm / Q000201:enzymology; D005947:Glucose / Q000032:analysis; D005959:Glucosidases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009088:Mucoproteins / Q000032:analysis; D009113:Muramidase / Q000378:metabolism; D009153:Mutagens; D009154:Mutation; D009604:Nitrosoguanidines; D010733:Phosphoglucomutase / Q000378:metabolism; D010758:Phosphorus / Q000032:analysis; D013385:Succinate Dehydrogenase / Q000378:metabolism
D000596:Amino Acids; D000606:Amino Sugars; D002241:Carbohydrates; D009088:Mucoproteins; D009153:Mutagens; D009604:Nitrosoguanidines; D010758:Phosphorus; D013385:Succinate Dehydrogenase; D005959:Glucosidases; D009113:Muramidase; D000581:Amidohydrolases; D010733:Phosphoglucomutase; D005947:Glucose