Chloral hydrate: a solvent for biological membranes.
A buffer system conitainin chloral hydrate, taurine, and bromopyridinium lactate was used to dissolve several biological membranes and separate their protein components by polyacrylamide gel electrophoresis. This solvent system was capable of separating molecules of similar size on the hasis of their charge and allows easy recovery of the proteins Thus. aqueous chloral hydrate is an effective solvent for biological membranes.
D000818:Animals; D002021:Buffers; D002417:Cattle; D002462:Cell Membrane; D002697:Chloral Hydrate; D004591:Electrophoresis, Polyacrylamide Gel; D008566:Membranes / Q000032:analysis; D051379:Mice; D011506:Proteins; D011726:Pyridinium Compounds; D012967:Sodium Dodecyl Sulfate; D012995:Solubility; D012997:Solvents; D013654:Taurine
D002021:Buffers; D011506:Proteins; D011726:Pyridinium Compounds; D012997:Solvents; D013654:Taurine; D012967:Sodium Dodecyl Sulfate; D002697:Chloral Hydrate