Evaluation of a solid phase radioimmunoassay (Ausria-125) for the detection of hepatitis B antigen (Australia antigen) in two clinical materials.
A solid-phase radioimmunoassay (Ausria-125) was compared to an immunoelectro-osmophoresis method in detecting hepatitis-B antigen (HBAg). The radioimmunoassay detected HBAg in approximately 230 times higher dilutions than the immunoelectroosmophoresis method. In cases of acute hepatitis HBAg could be detected for about 70% longer after the onset of jaundice using radioimmunoassay compared with immunoelectroosmophoresis. Of 138 patients with acute hepatitis, who were HBAg-negative by immunoelectroosmophoresis, 23 (17%) were positive by radioimmunoassay. The specificity of the positive results with this test was investigated by neutralization tests with human antiserum to HBAg.
D000818:Animals; D006041:Goats / Q000276:immunology; D006168:Guinea Pigs / Q000276:immunology; D006505:Hepatitis / Q000175:diagnosis; D006511:Hepatitis B Antigens / Q000032:analysis*; D006801:Humans; D007104:Immune Adherence Reaction; D007106:Immune Sera; D007122:Immunoelectrophoresis; D007457:Iodine Radioisotopes; D009995:Osmosis; D011233:Precipitin Tests; D011863:Radioimmunoassay
D006511:Hepatitis B Antigens; D007106:Immune Sera; D007457:Iodine Radioisotopes