Expression and localization of Escherichia coli alkaline phosphatase synthesized in Salmonella typhimurium cytoplasm.
The Escherichia coli structural gene for alkaline phosphatase was inserted into Salmonella typhimurium by episomal transfer in order to determine whether this enzyme would continue to be localized to the periplasmic space of the bacterium even though it was formed in a cell that does not synthesize alkaline phosphatase. The S. typhimurium heterogenote synthesized alkaline phosphatase under conditions identical to that observed with E. coli. This enzyme appeared to be identical to that synthesized by E. coli, and was quantitatively released from the bacterial cell by spheroplast formation with lysozyme. These results showed that localization is not a property unique to the E. coli cell and suggested that, in E. coli, enzyme location is related to the structure of the protein. Formation of alkaline phosphatase in the S. typhimurium heterogenote was repressed in cells growing in a medium with excess inorganic phosphate, even though only one of the three regulatory genes for this enzyme is on the episome. Thus, S. typhimurium can supply the products of the other two regulatory genes essential for repression even though this bacterium seems to lack the structural gene for alkaline phosphatase.
D000469:Alkaline Phosphatase / Q000096:biosynthesis* / Q000378:metabolism; D003227:Conjugation, Genetic; D003593:Cytoplasm / Q000201:enzymology; D004794:Enzyme Repression; D004926:Escherichia coli / Q000201:enzymology*; D005796:Genes; D005809:Genes, Regulator; D005827:Genetics, Microbial; D008967:Molecular Biology; D009113:Muramidase / Q000494:pharmacology; D010710:Phosphates / Q000494:pharmacology; D011523:Protoplasts; D012486:Salmonella typhimurium / Q000187:drug effects / Q000201:enzymology*
D010710:Phosphates; D000469:Alkaline Phosphatase; D009113:Muramidase