Cytokinin from soluble RNA of Escherichia coli: 6-(3-methyl-2-butenylamino)-2-methylthio-9-beta-D-ribofuranosylpurine.
We have isolated a compound responsible for the cytokinin activity of soluble RNA from Escherichia coli. The structure, indicated as 6-(3-methyl-2-butenylamino)-2-methylthio-9-beta-D-ribofuranosylpurine, C(16)H(23)N(5)0(4)S, on the basis of low-and high-reso!ution mass spectrometry, was established by unequivocal synthesis. The mass spectra, chromatographic behavior, and ultraviolet spectra of the compounds from natural and synthetic sources were identical.
D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D004926:Escherichia coli / Q000032:analysis*; D009705:Nucleosides / Q000032:analysis*; D010937:Plant Growth Regulators / Q000032:analysis*; D012329:RNA, Bacterial / Q000032:analysis; D012343:RNA, Transfer / Q000032:analysis*; D013057:Spectrum Analysis; D014466:Ultraviolet Rays
D009705:Nucleosides; D010937:Plant Growth Regulators; D012329:RNA, Bacterial; D012343:RNA, Transfer