title: Single crystals of transfer RNA from formylmethionine and phenylalanine transfer RNA's.
abstract: Reproducible conditions have been developed for crystallization of transfer RNA. The conditions may be applicable to many pure transfer RNA species since identical procedures (except for initial transfer-RNA concentration) yielded good crystals from both yeast and Escherichia coli transfer RNA. These crystals, which must be kept at temperatures below about 10 degrees C and handled in vapor of controlled alcohol concentration, have been studied by x-ray crystallography. The availability of crystals of a nucleic acid opens a route for extending knowledge of the tertiary structure of transfer RNA and its relation to important biological functions.
mesh_terms: D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003035:Cobalt; D003460:Crystallization; D003461:Crystallography; D004926:Escherichia coli; D005561:Formates; D008274:Magnesium; D008715:Methionine; D010649:Phenylalanine; D011189:Potassium Chloride; D000644:Quaternary Ammonium Compounds; D012329:RNA, Bacterial; D012343:RNA, Transfer / Q000032:analysis; D013431:Sulfates; D014961:X-Ray Diffraction; D015003:Yeasts
chemical_list: D005561:Formates; D000644:Quaternary Ammonium Compounds; D012329:RNA, Bacterial; D013431:Sulfates; D003035:Cobalt; D010649:Phenylalanine; D011189:Potassium Chloride; D012343:RNA, Transfer; D008715:Methionine; D008274:Magnesium