Differentiated rat glial cell strain in tissue culture.
Rat glial tumors, induced by injections of N-nitrosomethylurea, were plated and propagated in culture. Among a few cell strains obtained, one clone contains S-100 protein, which is unique to brain in vertebrates. Stationary-phase cultures contain approximately ten times more S-100 protein per cell than exponentially growing cells. When injected into newborn rats, cells producing S-100 grew as a glial tumor, which contained S-100 protein.
D000818:Animals; D001932:Brain Neoplasms / Q000139:chemically induced / Q000378:metabolism*; D002454:Cell Differentiation; D002999:Clone Cells; D046508:Culture Techniques; D005910:Glioma / Q000139:chemically induced / Q000378:metabolism*; D008858:Microscopy, Phase-Contrast; D009374:Neoplasms, Experimental / Q000139:chemically induced; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009603:Nitroso Compounds; D051381:Rats; D014508:Urea
D009419:Nerve Tissue Proteins; D009603:Nitroso Compounds; D014508:Urea