Selection of temperature-sensitive activating enzyme mutants in Escherichia coli.
A method based on temperature-conditional resistance to thymineless death has been designed to facilitate the isolation of amino acid-activating enzyme mutants. This method may be modified to obtain a greater or lesser proportion of activating enzyme mutants. In the latter instance, an increased proportion of temperature-sensitive macromolecule mutants of other types is obtained. Additional uses of this procedure are discussed.
D000596:Amino Acids / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis; D002247:Carbon Isotopes; D004926:Escherichia coli / Q000201:enzymology; D008025:Ligases / Q000378:metabolism*; D008967:Molecular Biology; D009154:Mutation; D011392:Proline / Q000378:metabolism; D012329:RNA, Bacterial / Q000096:biosynthesis; D013696:Temperature; D013941:Thymine / Q000378:metabolism; D014316:Tritium; D014498:Uracil / Q000378:metabolism
D000596:Amino Acids; D001426:Bacterial Proteins; D002247:Carbon Isotopes; D012329:RNA, Bacterial; D014316:Tritium; D014498:Uracil; D011392:Proline; D008025:Ligases; D013941:Thymine