title: Involvement of protein and ribonucleic acid in the association of deoxyribonucleic acid with other cell components of Escherichia coli.
abstract: Cells of Escherichia coli were labeled with precursors of ribonucleic acid (RNA), deoxyribonucleic acid (DNA), and protein, lysed with detergent, and examined by starch-block electrophoresis and CsCl density gradient centrifugation. A large amount of the DNA was seen to remain at positions of low electrophoretic mobility and light density along with tryptophan and arginine-containing proteins and some RNA. Addition of labeled, phenol-extracted DNA to unlabeled cells prior to lysis and electrophoresis showed that only a small amount of the DNA became associated during or after lysis. Sonic treatment of a lysate removed most of the DNA to a position of electrophoretic mobility and density similar to that of free DNA, whereas pronase and ribonuclease released only a part of the DNA. We concluded that binding of DNA to cell membranes or other cell components occurs in the cell prior to lysis and involves protein and probably a specific type of RNA.
mesh_terms: D001120:Arginine / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D001433:Bacteriolysis; D002247:Carbon Isotopes; D002462:Cell Membrane / Q000378:metabolism; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D004269:DNA, Bacterial; D003902:Detergents / Q000494:pharmacology; D004589:Electrophoresis, Disc; D004926:Escherichia coli / Q000378:metabolism; D012329:RNA, Bacterial / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D013941:Thymine / Q000378:metabolism; D014316:Tritium; D014364:Tryptophan / Q000378:metabolism; D014465:Ultrasonics; D014498:Uracil / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002247:Carbon Isotopes; D004269:DNA, Bacterial; D003902:Detergents; D012329:RNA, Bacterial; D014316:Tritium; D014498:Uracil; D014364:Tryptophan; D001120:Arginine; D013941:Thymine; D013936:Thymidine