The mechanism of action of methotrexate. I. Interaction with a low-affinity intracellular site required for maximum inhibition of deoxyribonucleic acid synthesis in L-cell mouse fibroblasts.
D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D004247:DNA / Q000037:antagonists & inhibitors* / Q000096:biosynthesis*; D003857:Deoxyuridine / Q000378:metabolism; D003864:Depression, Chemical; D004357:Drug Synergism; D005347:Fibroblasts / Q000378:metabolism; D007444:Inulin / Q000378:metabolism; D007700:Kinetics; D007739:L Cells / Q000378:metabolism*; D008727:Methotrexate / Q000378:metabolism / Q000494:pharmacology*; D051379:Mice; D013696:Temperature; D013763:Tetrahydrofolates / Q000494:pharmacology; D014316:Tritium; D014750:Vincristine / Q000494:pharmacology; D014867:Water / Q000378:metabolism
D002250:Carbon Radioisotopes; D013763:Tetrahydrofolates; D014867:Water; D014316:Tritium; D014750:Vincristine; D007444:Inulin; D004247:DNA; D003857:Deoxyuridine; D008727:Methotrexate