Continuous production of Clostridium tetani toxin.
The continuous production of Clostridium tetani toxin has been carried out in a 1-liter stirred culture vessel for as long as 65 days. Toxin production of approximately 120 flocculating units per ml was maintained with a dilution rate of 0.125 hr(-1), a temperature of 34 C, a pH of 7.4, and the addition to the medium of 0.1 g of potassium chloride per liter. The average minimal lethal intraperitoneal dose of the toxin in mice was approximately 10(6) per ml.
D001431:Bacteriological Techniques; D002122:Calcium Chloride / Q000494:pharmacology; D003017:Clostridium tetani / Q000378:metabolism*; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D011189:Potassium Chloride / Q000494:pharmacology; D013696:Temperature; D013744:Tetanus Toxin / Q000096:biosynthesis*
D003470:Culture Media; D013744:Tetanus Toxin; D011189:Potassium Chloride; D002122:Calcium Chloride