Characteristics of Clostridium botulinum type F isolated from the Pacific Coast of the United States.
Some of the physiological and biochemical characteristics of a type F strain recently isolated from the United States were studied and compared with those of the prototype Langeland type F strain. The recent isolates were nonproteolytic, fermented sucrose and ribose, produced spores of low thermal resistance, produced a protoxin activated by trypsin, and grew and produced toxin at 38 F (3.3 C) from a spore inoculum. The prototype Langeland strain was proteolytic, did not ferment sucrose or ribose, and produced spores of relatively high thermal resistance, and the toxin of 3-day-old cultures was not activated by trypsin. Approximately two to three times the minimal lethal dose (MLD) of type F toxin from either Langeland or nonproteolytic strains was cross-neutralized by 1,000 anti-MLD of type E antitoxin. Antitoxin serums prepared by immunizing rabbits with the toxoid of the nonproteolytic type F isolate neutralized the toxin of the Langeland strain, but did not show cross-neutralization with the toxins of other types of Clostridium botulinum.
D000818:Animals; D000992:Antitoxins; D050260:Carbohydrate Metabolism; D003014:Clostridium botulinum / Q000166:cytology / Q000302:isolation & purification* / Q000378:metabolism; D003080:Cold Temperature; D003470:Culture Media; D007211:Indoles / Q000096:biosynthesis; D051379:Mice; D009500:Neutralization Tests; D009566:Nitrates / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D011817:Rabbits; D012266:Ribose / Q000378:metabolism; D013170:Spores / Q000254:growth & development; D013395:Sucrose / Q000378:metabolism; D013440:Sulfides / Q000096:biosynthesis; D013997:Time Factors; D014118:Toxins, Biological / Q000096:biosynthesis / Q000633:toxicity; D014357:Trypsin / Q000494:pharmacology; D014481:United States; D014510:Urease / Q000096:biosynthesis
D000992:Antitoxins; D003470:Culture Media; D007211:Indoles; D009566:Nitrates; D011506:Proteins; D013440:Sulfides; D014118:Toxins, Biological; D013395:Sucrose; D012266:Ribose; D014357:Trypsin; D014510:Urease