title: Cell growth and division. II. Experimental studies of cell volume distributions in mammalian suspension cultures.
abstract: Experimental proof is given that the volume distribution spectrum of mammalian cells in suspension culture can be determined accurately with a Coulter spectrometer. Stable spectra corresponding to the predictions of a mathematical model are observed under favorable conditions of growth. Cell volume spectrometry appears to be a useful method for diagnosing the state of the culture with respect to past uniformity of growth rate and present population age distribution. In addition, it offers a method for quantitative study of the laws governing cell growth and division.
mesh_terms: D000818:Animals; D002455:Cell Division; D006224:Cricetinae; D046508:Culture Techniques; D005347:Fibroblasts / Q000166:cytology; D008223:Lymphoma / Q000473:pathology; D051379:Mice; D008858:Microscopy, Phase-Contrast; D011058:Pollen / Q000166:cytology; D011158:Population Growth; D013057:Spectrum Analysis