Compatibility of trypsin-modified human erythrocytes in the rubella hemagglutination-inhibition test employing three serum treatment procedures.
Results of comparative tests using trypsin-modified human type O erythrocytes and cells from newly hatched chickens with three standard serum treatment methods for rubella hemagglutination-inhibition techniques are reported. The kaolin, heparin-manganous chloride and dextran sulfate-calcium chloride methods could all be used with both cell types. Reproducibility with heparin-manganous chloride and dextran sulfate-calcium chloride was excellent with both cell types. Both methods gave generally higher antibody titers than the kaolin procedure. However, the use of human cells resulted in a more sensitive system than chicken cells with all serum treatment methods.
D000818:Animals; D001789:Blood Group Antigens; D002122:Calcium Chloride; D002645:Chickens / Q000276:immunology; D002712:Chlorides; D003911:Dextrans; D004912:Erythrocytes / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests; D006493:Heparin; D006801:Humans; D007202:Indicators and Reagents; D007616:Kaolin; D008345:Manganese; D008722:Methods; D012409:Rubella / Q000276:immunology*; D012412:Rubella virus / Q000276:immunology*; D014357:Trypsin
D001789:Blood Group Antigens; D002712:Chlorides; D003911:Dextrans; D007202:Indicators and Reagents; D007616:Kaolin; D008345:Manganese; D006493:Heparin; D014357:Trypsin; D002122:Calcium Chloride