Production of alkaline phosphatase from Escherichia coli in continuous culture.
A 2-liter continuous culture for the production of Escherichia coli rich in alkaline phosphatase is described. The maximal output is greater than 250 mg of enzyme per day. The enzyme yield per unit of bacterial dry weight is only one-fifth of that obtained in earlier investigations. However, because of the increased bacterial density, the output per unit volume of culture is more than four times as great.
D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D001431:Bacteriological Techniques; D003470:Culture Media; D004926:Escherichia coli / Q000201:enzymology*
D003470:Culture Media; D000469:Alkaline Phosphatase