Microtiter indirect hemagglutination procedure for identification of streptococcal M-protein antibodies.
A number of investigators have attempted to utilize the hemagglutination system for detection of streptococcal type-specific antibody in human sera. Cross-reactions have made the procedure unreliable without cumbersome and time-consuming manipulation of the test sera. A method is described in which a microtiter indirect hemagglutination technique, using sensitized sheep erythrocytes, is sensitive, specific, and reliable for titration of type-specific antibody after naturally acquired or induced streptococcal infection.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000937:Antigen-Antibody Reactions; D001426:Bacterial Proteins; D001770:Blood Bactericidal Activity; D004912:Erythrocytes; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D015186:Hominidae; D006801:Humans; D007106:Immune Sera; D008722:Methods; D011817:Rabbits; D012756:Sheep; D013290:Streptococcal Infections / Q000276:immunology; D013297:Streptococcus pyogenes / Q000276:immunology*
D000906:Antibodies; D001426:Bacterial Proteins; D007106:Immune Sera