title: Physiological and serological comparisons among strains of Mycoplasma granularum and Mycoplasma laidlawii.
abstract: Mycoplasma granularum strains grew on a medium devoid of animal serum or of serum fractions containing sterols; all strains possessed properties, including carotenoid biosynthesis, similar to those described for M. laidlawii. Some common antigenic components were noted among M. granularum and M. laidlawii strains by indirect fluorescent-antibody tests. The growth of M. granularum strains was slightly inhibited by antiserum to M. laidlawii PG-8, and the electrophoretic patterns of cell proteins of the M. granularum strains showed a close resemblance to that of M. laidlawii. However, direct fluorescent-antibody procedures performed on colonies grown on a serum-free medium clearly distinguished M. granularum from M. laidlawii. The occurrence of nonsterol-requiring mycoplasmas, in addition to M. laidlawii, raises questions as to the taxonomy of M. granularum and of the saprophytic mycoplasmas in general.
mesh_terms: D000818:Animals; D000941:Antigens / Q000032:analysis; D001426:Bacterial Proteins / Q000032:analysis; D002338:Carotenoids / Q000096:biosynthesis; D002784:Cholesterol / Q000378:metabolism; D003470:Culture Media; D046508:Culture Techniques; D004589:Electrophoresis, Disc; D005455:Fluorescent Antibody Technique; D009174:Mycoplasma / Q000145:classification / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D009666:Nose / Q000382:microbiology; D013552:Swine
chemical_list: D000941:Antigens; D001426:Bacterial Proteins; D003470:Culture Media; D002338:Carotenoids; D002784:Cholesterol