Extraction and isolation of individual ribosomal proteins from Escherichia coli.
We have described a new method for the quantitative separation of ribosomal proteins and ribosomal ribonucleic acid. A procedure for the preparation of individual ribosomal proteins by polyacrylamide gel electrophoresis is also described. By the use of gels with smaller pores, at least four of the electrophoretic components from the 30S ribosome can be split into additional protein fractions. By the methods described here, it is possible to isolate in high purity at least 15 different proteins from the 30S ribosome of Escherichia coli.
D001426:Bacterial Proteins / Q000032:analysis*; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D003720:Densitometry; D004589:Electrophoresis, Disc / Q000295:instrumentation; D004926:Escherichia coli / Q000032:analysis*; D008722:Methods; D012329:RNA, Bacterial / Q000032:analysis; D012270:Ribosomes / Q000032:analysis*
D001426:Bacterial Proteins; D012329:RNA, Bacterial