The sequence of amino acids in insulin isolated from islet tissue of the cod (Gadus callarias).
1. S-Aminoethylcysteinyl derivatives of the A and B chains of cod insulin were prepared from the individual S-sulpho chains. 2. Studies on small peptides derived from the S-aminoethylated peptide chains by treatment with trypsin allowed the amino acid sequences in the region of the cysteinyl residues of the A and B peptide chains to be defined. 3. The six amide groups in cod insulin were located by complete digestion of small peptides from the A and B chains with aminopeptidase followed by amino acid analyses. 4. The results, together with previous studies on the oxidized A and B chains, define the sequences of the 51 amino acids that constitute cod insulin.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000626:Aminopeptidases; D000818:Animals; D005399:Fishes; D007328:Insulin / Q000032:analysis*; D007515:Islets of Langerhans / Q000032:analysis; D010455:Peptides / Q000378:metabolism
D000596:Amino Acids; D007328:Insulin; D010455:Peptides; D000626:Aminopeptidases