Detection of Salmonella by a single-culture technique.
Dulcitol-selenite enrichment medium in a motility flask was used for the detection of Salmonella in food. A drop in pH of the dulcitol-selenite enrichment motility broth indicated the presence of Salmonella; this phenomenon was confirmed by fluorescent-antibody staining. A complete correlation was found between fluorescent-antibody staining and recovery on Brilliant Green agar. Testing of 332 samples of 8 different kinds of foods and feeds indicated no significant difference in sensitivity between the new technique and a conventional Salmonella detection technique. The new technique permitted detection of even small numbers of Salmonella in 1 to 2 days.
D000438:Alcohols; D000821:Animal Feed; D000818:Animals; D001431:Bacteriological Techniques; D003470:Culture Media; D003553:Cystine; D004531:Eggs; D005396:Fish Products; D005455:Fluorescent Antibody Technique; D005516:Food Microbiology; D008460:Meat; D008892:Milk; D011202:Poultry Products; D012475:Salmonella; D012643:Selenium
D000438:Alcohols; D003470:Culture Media; D003553:Cystine; D012643:Selenium