title: RNA-DNA hybridization: demonstration in a mammalian system of competition by preincubation.
abstract: Nonspecific intermolecular interactions obscure the interpretation of competition experiments involving hybridization of DNA and RNA in mammalian systems when the labeled RNA/DNA ratio is low. The effect of these interactions can be minimized by choosing a sufficiently high ratio of labeled RNA/DNA. No serious effect of nonspecific interactions is observed in bacteriophage systems, even at very low labeled RNA/DNA ratios.
mesh_terms: D000818:Animals; D001665:Binding Sites; D055598:Chemical Phenomena; D002621:Chemistry; D003090:Coliphages; D004247:DNA; D004269:DNA, Bacterial; D004279:DNA, Viral; D004926:Escherichia coli; D006824:Hybridization, Genetic; D007739:L Cells; D051379:Mice; D012313:RNA; D012367:RNA, Viral; D014316:Tritium
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral; D012367:RNA, Viral; D014316:Tritium; D012313:RNA; D004247:DNA