Fluorescent-antibody reagent for the identification of Cryptococcus neoformans.
A sensitive and diagnostically applicable conjugate for the rapid and accurate detection and identification of Cryptococcus neoformans has been developed. C. neoformans rabbit antisera were produced by 14 daily intravenous injections of 36 million cells for a total dosage of approximately 500 million cells. Cross-staining reactions with species of Cryptococcus other than C. neoformans, as well as with Candida species, were eliminated by adsorption of the C. neoformans conjugate with cells of C. diffluens and C. krusei.
D000327:Adsorption; D000818:Animals; D000906:Antibodies; D003454:Cryptococcus / Q000276:immunology / Q000302:isolation & purification*; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D008722:Methods; D011817:Rabbits
D000906:Antibodies; D007106:Immune Sera