Comparison of purified alpha-toxins from various strains of Staphylococcus aureus.
Alpha-toxin from five strains of Staphylococcus aureus, including Wood 46, was purified by isoelectric focusing. The alpha-toxins obtained from different strains were similar. The isoelectric point of the purified toxins was 8.65 +/- 0.15. Sharp concentration peaks were not always obtained. In the ultracentrifuge the alpha-toxins migrated usually as three peaks which could be dissociated with propionic acid to yield one peak. A single line of identity was obtained in immunoelectrophoresis when a heterologous antiserum was reacted with the five purified toxins. It was concluded that the widespread use of the Wood 46 strain for the production of alpha-toxin is justified.
D000818:Animals; D004912:Erythrocytes / Q000276:immunology; D006460:Hemolysin Proteins / Q000032:analysis* / Q000302:isolation & purification; D006461:Hemolysis; D007122:Immunoelectrophoresis; D007525:Isoelectric Focusing; D011817:Rabbits; D013045:Species Specificity; D013210:Staphylococcus / Q000032:analysis*; D014461:Ultracentrifugation
D006460:Hemolysin Proteins