Isoleucine and valine metabolism of Escherichia coli. XIV. Effect of thiaisoleucine.
Thiaisoleucine (2-amino-3-methylthiobutyrate) completely inhibited the growth of strain K-12 of Escherichia coli at a concentration of 5 x 10(-3)m. The inhibition was antagonized by growth-factor amounts of l-isoleucine. Thiaisoleucine inhibited the deamination of threonine and the transfer of (14)C-isoleucine to soluble ribonucleic acid and underwent transamination with alpha-ketoglutarate as the amino acceptor. In each case, the analogue appeared to be less effective than isoleucine as either an inhibitor or substrate.
D002247:Carbon Isotopes; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D006836:Hydro-Lyases / Q000378:metabolism; D007532:Isoleucine / Q000378:metabolism* / Q000494:pharmacology; D013912:Threonine / Q000378:metabolism; D000637:Transaminases / Q000378:metabolism; D014633:Valine / Q000378:metabolism*
D002247:Carbon Isotopes; D007532:Isoleucine; D013912:Threonine; D000637:Transaminases; D006836:Hydro-Lyases; D014633:Valine