Surface structure of yeast protoplasts.
The fine structure of the yeast cell wall during protoplast formation was studied by means of phase-contrast microscopy and the freeze-etching technique. The freeze-etching results indicated that at least in some cases the entire wall substance was not removed from the surface of the protoplasts. After a treatment of 30 min to 3 hr with 2% snail enzymes, an innermost thin wall layer as well as remnants of the fibrillar middle layer sometimes could be demonstrated.
D002462:Cell Membrane; D002473:Cell Wall; D005615:Freezing; D006652:Histological Techniques; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D011523:Protoplasts / Q000166:cytology*; D012440:Saccharomyces / Q000166:cytology; D015003:Yeasts / Q000166:cytology*