Effects of p-fluorophenylalanine and chloramphenicol on chemotaxis in Escherichia coli.
The effects of chloramphenicol and p-fluorophenylalanine (p-FPA) on growth, proportion of motile cells, average rate of motility, and the chemotactic response of a methionine auxotroph of Escherichia coli K-12 were studied. Kinetic studies revealed that the inhibition of chemotaxis by p-FPA can be explained by the effect on growth, proportion of motile cells, and average rate of motility rather than a selective inhibition of chemotaxis per se. The effect of chloramphenicol on chemotaxis could not be explained in terms of these characteristics. It is concluded that low concentrations of chloramphenicol, unlike p-FPA, selectively inhibit chemotaxis.
D003585:Cell Biology / Q000187:drug effects*; D002633:Chemotaxis / Q000187:drug effects; D002701:Chloramphenicol / Q000494:pharmacology*; D003470:Culture Media; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D005461:Fluorine / Q000494:pharmacology; D005690:Galactose / Q000378:metabolism; D007700:Kinetics; D008715:Methionine / Q000378:metabolism; D009068:Movement / Q000187:drug effects; D010649:Phenylalanine / Q000494:pharmacology*
D003470:Culture Media; D005461:Fluorine; D010649:Phenylalanine; D002701:Chloramphenicol; D008715:Methionine; D005690:Galactose