Maintenance of fiable segments of human artery in vitro.
A system for maintaining viable segments of atherosclerotic human artery in vitro for periods of up to 32 days is described. Viability of the segment has been confirmed from study of lactate metabolism and vessel wall histology. Preliminary lipid studies using TLC on plasma perfusate indicate lipid may be removed from the vessel wall during perfusion.
D000818:Animals; D000900:Anti-Bacterial Agents; D001158:Arteries / Q000378:metabolism / Q000473:pathology; D001161:Arteriosclerosis / Q000473:pathology*; D002102:Cadaver; D002417:Cattle; D002855:Chromatography, Thin Layer; D003470:Culture Media; D006801:Humans; D066298:In Vitro Techniques; D007773:Lactates / Q000378:metabolism; D008055:Lipids / Q000032:analysis / Q000097:blood; D010100:Oxygen / Q000097:blood; D010106:Oxygenators; D010477:Perfusion; D013696:Temperature; D014021:Tissue Preservation / Q000295:instrumentation; D014280:Triglycerides / Q000032:analysis / Q000097:blood
D000900:Anti-Bacterial Agents; D003470:Culture Media; D007773:Lactates; D008055:Lipids; D014280:Triglycerides; D010100:Oxygen