Nonspecific staining: its control in immunofluorescence examination of soil.
Gelatin preparations were used to treat soil slides prior to addition of fluorescent antibody. Nonspecific staining was avoided, with no detectable interference to specific staining. Gelatin-rhodamine conjugates served to counterstain as well as to prevent nonspecific staining.
D000818:Animals; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D005604:Francisella tularensis / Q000276:immunology; D005780:Gelatin; D008099:Liver / Q000473:pathology; D051379:Mice; D010326:Pasteurella Infections / Q000473:pathology; D012231:Rhizobium / Q000276:immunology*; D012988:Soil Microbiology; D013154:Spleen / Q000473:pathology; D013861:Thiocyanates
D005456:Fluorescent Dyes; D013861:Thiocyanates; D005780:Gelatin