title: Genetic and biochemical studies on mannose-negative mutants that are deficient in phosphomannose isomerase in Escherichia coli K-12.
abstract: Two mannose-negative mutants of Escherichia coli K-12 have been isolated. These mutants are deficient in the ability to synthesize phosphomannose isomerase and capsular polysaccharide when grown on glucose-containing media. Interrupted mating experiments to determine the kinetics of genetic transfer show that the two mannose-negative mutations map together between the histidine and tryptophan regions of the E. coli chromosome.
mesh_terms: D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development; D005827:Genetics, Microbial; D005947:Glucose / Q000378:metabolism; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006639:Histidine; D007535:Isomerases / Q000096:biosynthesis / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism; D009154:Mutation; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D011995:Recombination, Genetic; D013307:Streptomycin / Q000494:pharmacology; D014364:Tryptophan
chemical_list: D011135:Polysaccharides, Bacterial; D006639:Histidine; D014364:Tryptophan; D005954:Glucosephosphate Dehydrogenase; D007535:Isomerases; D005956:Glucose-6-Phosphate Isomerase; D005947:Glucose; D008358:Mannose; D013307:Streptomycin