title: Behavior of the NO-beta-D-glucosiduronic acid of N-acetyl-N-phenylhydroxylamine as a substrate for beta-glucuronidase.
abstract: 1. Biosynthetic sodium (N-acetyl-N-phenylhydroxylamine NO-beta-d-glucosid)-uronate is hydrolysed completely by purified mouse urinary beta-glucuronidase into the products N-acetyl-N-phenylhydroxylamine and glucuronic acid. The hydrolysis is inhibited by saccharo-(1-->4)-lactone. These results not only confirm the identity and purity of the substrate but also establish it as a substrate for beta-glucuronidase. 2. Mammalian and bacterial beta-glucuronidase preparations hydrolysed the substrate at a rate one-fifth of that for (phenolphthalein beta-d-glucosid)uronic acid under the optimum conditions of hydrolysis for each source. 3. The pH optimum is 4.1 and the Michaelis constant, K(m), is 3.3x10(-4)m with purified mouse urinary beta-glucuronidase as the enzyme source acting on the NO-beta-d-glucosiduronic acid. The aglycone after extraction into chloroform was quantitatively determined spectrophotometrically at its absorption maximum (256mmu). 4. The hydrolysis was studied as a function of time and temperature. 5. From a consideration of the chemical and enzymic properties of this NO-beta-d-glucosiduronic acid it is possible to suggest its catabolism in vivo.
mesh_terms: D000818:Animals; D004926:Escherichia coli / Q000201:enzymology; D005966:Glucuronidase / Q000652:urine; D006027:Glycosides; D006863:Hydrogen-Ion Concentration; D006898:Hydroxylamines; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D007783:Lactones; D007865:Leeches / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D051379:Mice; D012908:Snails / Q000201:enzymology; D013053:Spectrophotometry; D013696:Temperature; D014574:Uronic Acids
chemical_list: D006027:Glycosides; D006898:Hydroxylamines; D007783:Lactones; D014574:Uronic Acids; D005966:Glucuronidase