An upper limit on beta-galactosidase transfer in bacterial conjugation.
An upper limit for beta-galactosidase transfer between mating F(+) and F(-)Escherichia coli has been determined by a new technique which relies on selective lysis of the donor strain by heat induction of a thermo-inducible strain of lambda, accompanied by chymotryptic digestion of the released beta-galactosidase. No significant transfer of beta-galactosidase during mating between F(+) and F(-) cells has been observed: 0.05 +/- 0.05% of the enzyme originally present in the male cells is found in the female cells after 1 hr of mating at 37 C.
D001433:Bacteriolysis; D002918:Chymotrypsin / Q000378:metabolism; D003090:Coliphages / Q000378:metabolism; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000201:enzymology*; D005696:Galactosidases / Q000378:metabolism; D006358:Hot Temperature; D014161:Transduction, Genetic
D005696:Galactosidases; D002918:Chymotrypsin