Microculture system for detection of Newcastle disease virus antibodies.
A microculture system utilizing cytopathic effect (CPE) and hemadsorption (HAd) end points was effective in determining the level of Newcastle disease virus (NDV) antibodies. The microculture system was of comparable sensitivity to the plaque reduction test for the detection of NDV antibodies. The standards by which the CPE and HAd microculture tests would be considered reproducible were defined. The results indicate that the CPE and HAd microculture tests are reproducible within one twofold dilution.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002645:Chickens; D046508:Culture Techniques; D003588:Cytopathogenic Effect, Viral; D005069:Evaluation Studies as Topic; D006382:Hemadsorption; D007668:Kidney; D008722:Methods; D009500:Neutralization Tests; D009522:Newcastle disease virus / Q000254:growth & development / Q000276:immunology*; D013223:Statistics as Topic; D014776:Virus Cultivation
D000914:Antibodies, Viral