title: Genetic analysis of carbohydrate transport-deficient mutants of Salmonella typhimurium.
abstract: Mutants (car) isolated from Salmonella typhimurium were unable to utilize or ferment the following carbohydrates (all d-configuration): glucose, fructose, mannose, N-acetylglucosamine, sorbitol, mannitol, maltose, melibiose, and glycerol. The mutants did utilize galactose, glucose 6-phosphate, gluconic acid, glucuronic acid, pyruvate, and l-lactate. Biochemical analysis showed that there were two classes of mutants, each lacking one component of a phosphotransferase system. CarA mutants were deficient in enzyme I; carB lacked the phosphate carrier protein, HPr. Mapping experiments showed that the carA gene was located near pro; the carB gene mapped near purC.
mesh_terms: D001692:Biological Transport; D050260:Carbohydrate Metabolism; D002874:Chromosome Mapping; D004187:Disaccharides / Q000378:metabolism; D005285:Fermentation; D005632:Fructose / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D005827:Genetics, Microbial; D005942:Gluconates / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005965:Glucuronates / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006600:Hexosephosphates / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D008320:Maltose / Q000378:metabolism; D008353:Mannitol / Q000378:metabolism; D008358:Mannose / Q000378:metabolism; D009154:Mutation; D010770:Phosphotransferases / Q000096:biosynthesis; D011773:Pyruvates / Q000378:metabolism; D012486:Salmonella typhimurium / Q000378:metabolism*; D013012:Sorbitol / Q000378:metabolism
chemical_list: D004187:Disaccharides; D005942:Gluconates; D005965:Glucuronates; D006600:Hexosephosphates; D007773:Lactates; D011773:Pyruvates; D005632:Fructose; D008353:Mannitol; D013012:Sorbitol; D008320:Maltose; D010770:Phosphotransferases; D005947:Glucose; D005944:Glucosamine; D005990:Glycerol; D008358:Mannose; D005690:Galactose