Acyl carrier protein: effects of acetylation and tryptic hydrolysis on function in fatty acid synthesis.
Acetylation of the four lysine residues and the amino group of the terminal serine residue of Escherichia coli acyl carrier protein has no effect on the ability of this protein to function in fatty acid synthesis. Subsequent trypsin hydrolysis resulting in complete inactivation cleaves a single arginyl peptide bond, releasing the amino terminal hexapeptide from the molecule.
D000085:Acetates / Q000378:metabolism*; D000596:Amino Acids / Q000032:analysis; D001426:Bacterial Proteins / Q000378:metabolism*; D002247:Carbon Isotopes; D002268:Carboxypeptidases; D002850:Chromatography, Gel; D004926:Escherichia coli / Q000378:metabolism*; D005227:Fatty Acids / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D008239:Lysine / Q000378:metabolism; D010455:Peptides / Q000032:analysis / Q000378:metabolism; D012694:Serine / Q000378:metabolism; D014316:Tritium; D014357:Trypsin
D000085:Acetates; D000596:Amino Acids; D001426:Bacterial Proteins; D002247:Carbon Isotopes; D005227:Fatty Acids; D010455:Peptides; D014316:Tritium; D012694:Serine; D002268:Carboxypeptidases; D014357:Trypsin; D008239:Lysine