Chromosome replication in Salmonella typhimurium.
The replication of the Salmonella typhimurium chromosome was studied. As with E. coli 15T(-), replication was sequential. After amino acid starvation, replication proceeded from a unique and heritable region of the chromosome. 5-Bromouracil, when substituted for thymine, did not disturb the sequence of replication nor did it initiate extra replication cycles. By labeling the origin and the terminus of the chromosome with (3)H- and (14)C-thymine, respectively, it was possible to determine that the rate of chain elongation decreases as the growth rate decreases. No gap in the replication cycle could be observed.
D000596:Amino Acids / Q000378:metabolism; D001345:Autoradiography; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D002876:Chromosomes, Bacterial; D003227:Conjugation, Genetic; D004261:DNA Replication; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli; D009584:Nitrogen / Q000378:metabolism; D011868:Radioisotopes; D012486:Salmonella typhimurium / Q000254:growth & development; D013941:Thymine / Q000378:metabolism; D014316:Tritium; D014498:Uracil / Q000378:metabolism / Q000494:pharmacology
D000596:Amino Acids; D002247:Carbon Isotopes; D004269:DNA, Bacterial; D011868:Radioisotopes; D014316:Tritium; D014498:Uracil; D009584:Nitrogen; D013941:Thymine