Inhibition of herpes virus-induced cell fusion by concanavalin A, antisera, and 2-deoxy-D-glucose.
Pseudorabies virus-induced cell fusion in rabbit kidney cells can be prevented by Concanavalin A added early after infection. The infected cells are not agglutinated and the infectivity of cell-free virus is not reduced. Sera from productively infected animals also inhibit polykaryocytosis, whereas a hyperimmune serum directed against virus structural components has no effect. 2-Deoxy-d-glucose reversibly disturbs virus-induced fusion and reduces significantly the virus infectivity.
D000372:Agglutination Tests; D000818:Animals; D002459:Cell Fusion / Q000187:drug effects*; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology*; D046508:Culture Techniques; D004279:DNA, Viral / Q000096:biosynthesis; D005947:Glucose / Q000494:pharmacology*; D006564:Herpesviridae / Q000254:growth & development* / Q000378:metabolism; D007106:Immune Sera / Q000494:pharmacology*; D007668:Kidney; D008854:Microscopy, Electron; D011557:Pseudorabies / Q000382:microbiology*; D011817:Rabbits; D013552:Swine / Q000276:immunology
D004279:DNA, Viral; D007106:Immune Sera; D003208:Concanavalin A; D005947:Glucose