title: Biosynthesis of the O antigen from Citrobacter 139.
abstract: The biosynthesis of the O antigen of Citrobacter 139 (Escherichia coli 3 Zurich 4,5,12:z(20)) was shown to proceed through a series of lipid-linked intermediates, similar to those involved in O-antigen synthesis in Salmonella. Galactose was the first sugar incorporated, followed by rhamnose and mannose. Abequose was incorporated from cytidine diphosphate (CDP)-abequose only when all three of the other nucleotide sugars (uridine diphosphate galactose, guanosine diphosphate mannose, and thymidine diphosphate rhamnose) were present. Rhamnosyl-galactosyl 1-phosphate and mannosyl-rhamnosyl-galactosyl 1-phosphate were identified as the products of mild alkaline hydrolysis of the lipid-linked intermediates.
mesh_terms: D000941:Antigens / Q000096:biosynthesis*; D002247:Carbon Isotopes; D002850:Chromatography, Gel; D004586:Electrophoresis; D004926:Escherichia coli / Q000276:immunology* / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D008358:Mannose / Q000378:metabolism; D009005:Monosaccharides / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000032:analysis; D012210:Rhamnose / Q000378:metabolism
chemical_list: D000941:Antigens; D002247:Carbon Isotopes; D009005:Monosaccharides; D011135:Polysaccharides, Bacterial; D008358:Mannose; D012210:Rhamnose; D005690:Galactose