title: Nonspecific staining: its control in immunofluorescence examination of soil.
abstract: Gelatin preparations were used to treat soil slides prior to addition of fluorescent antibody. Nonspecific staining was avoided, with no detectable interference to specific staining. Gelatin-rhodamine conjugates served to counterstain as well as to prevent nonspecific staining.
mesh_terms: D000818:Animals; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D005604:Francisella tularensis / Q000276:immunology; D005780:Gelatin; D008099:Liver / Q000473:pathology; D051379:Mice; D010326:Pasteurella Infections / Q000473:pathology; D012231:Rhizobium / Q000276:immunology*; D012988:Soil Microbiology; D013154:Spleen / Q000473:pathology; D013861:Thiocyanates
chemical_list: D005456:Fluorescent Dyes; D013861:Thiocyanates; D005780:Gelatin