Ribosomal precursors and ribonucleic acid synthesis in Escherichia coli.
Ribosomes and immature ribonucleoprotein particles were isolated from extracts of log-phase cells grown under various conditions. Quantitative measurements were made to determine the relative amounts of immature particles present in the extracts. The results indicate that the steady-state level of ribosomal precursors accounted for essentially a constant fraction of the total ribonucleic acid (RNA) of the cells. For cells with RNA-protein ratios between 0.43 and 0.65, about 1.6% of the total RNA occurred as immature ribonucleoprotein particles. Further, increased levels of immature particles were shown to be correlated with a reduced rate of RNA synthesis in cells recovering from chloramphenicol inhibition. The reduction was found to vary directly with the duration of pretreatment in chloramphenicol and, consequently, with the level of immature particles present in the cells.
D002499:Centrifugation, Density Gradient; D002701:Chloramphenicol / Q000494:pharmacology; D003203:Computers, Hybrid; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D010710:Phosphates / Q000494:pharmacology; D012329:RNA, Bacterial / Q000032:analysis / Q000096:biosynthesis*; D012260:Ribonucleases; D012270:Ribosomes / Q000032:analysis / Q000378:metabolism*
D010710:Phosphates; D012329:RNA, Bacterial; D002701:Chloramphenicol; D012260:Ribonucleases