P1-mediated transduction of a gene that controls radiation sensitivity and capsular polysaccharide synthesis from Shigella dysenteriae to Escherichia coli.
When Shigella dysenteriae strain 60 is used as a donor and Escherichia coli K-12 strains that are ultraviolet (UV)-sensitive, mucoid, and proline-requiring (Pro(-)) are employed as recipients, selection for Pro(+) yields 2 to 6% nonmucoid clones. All of the nonmucoid clones examined are UV-resistant. Most of the nonmucoid UV-resistant transductants are partial diploids for the genes being studied. When these Shigella-Escherichia hybrids are used as donors with the same E. coli recipients, the cotransduction of Pro(+) and nonmucoidness is greatly increased (59 to 94% cotransduction). All of these nonmucoid transductants examined were also UV-resistant. The results indicate that Shigella contains an allele (designated ShproC(+)) homologous to proC of E. coli and a second linked allele (designated ShcapR(+)) homologous to the capR allele of E. coli. The ShcapR(+) allele changes the phenotype of certain E. coli strains from mucoid UV-sensitive (capR6) or very sensitive (capR9) to nonmucoid and UV-resistant. Unanticipated capR allele interactions in the partial diploid hybrids are described.
D000483:Alleles; D004171:Diploidy; D004926:Escherichia coli / Q000528:radiation effects; D005796:Genes; D006824:Hybridization, Genetic; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D011392:Proline / Q000378:metabolism; D011831:Radiation Genetics; D011995:Recombination, Genetic; D012641:Selection, Genetic; D012762:Shigella dysenteriae; D014161:Transduction, Genetic; D014466:Ultraviolet Rays
D011135:Polysaccharides, Bacterial; D011392:Proline