Analysis of the in vitro product of an RNA-dependent RNA polymerase isolated from influenza virus-infected cells.
The products synthesized in vitro by an RNA-dependent RNA polymerase isolated from influenza virus-infected BHK21-F cells were analyzed by velocity sedimentation, annealing techniques, and acrylamide-agarose gel electrophoresis. Approximately 50% of the RNA synthesized in vitro remains associated with the 50 to 70S ribonucleoprotein complex containing polymerase activity; the remainder of the RNA polymerase product sediments heterogeneously with a peak at 13S. At least 90% of the in vitro product hybridizes with virion RNA. If polypeptides are labeled early in the growth cycle, both the P and NP polypeptides are detected in the ribonucleoprotein complex by acrylamide gel electrophoresis. The results suggest that the polypeptide composition and the products of the cell-associated RNA polymerase are similar to those of the RNA transcriptase associated with influenza virus particles.
D000818:Animals; D002250:Carbon Radioisotopes; D002460:Cell Line; D002474:Cell-Free System; D006224:Cricetinae; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007668:Kidney; D009693:Nucleic Acid Hybridization; D009698:Nucleoproteins / Q000032:analysis; D009975:Orthomyxoviridae / Q000032:analysis* / Q000254:growth & development; D010455:Peptides / Q000032:analysis; D010761:Phosphorus Radioisotopes; D012367:RNA, Viral / Q000032:analysis* / Q000096:biosynthesis; D014500:Uracil Nucleotides / Q000378:metabolism; D014764:Viral Proteins / Q000032:analysis
D002250:Carbon Radioisotopes; D009698:Nucleoproteins; D010455:Peptides; D010761:Phosphorus Radioisotopes; D012367:RNA, Viral; D014500:Uracil Nucleotides; D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases