Linkage analysis in Dictyostelium discoideum using temperature-sensitive growth mutants selected with bromodeoxyuridine.
Amoebae of the cellular slime mold Dictyostelium discoideum grown in the presence of bromodeoxyuridine are killed on exposure to near-ultraviolet light. By using this phenomenon, a method was devised by which mutants of D. discoideum that are temperature-sensitive for growth can be readily obtained. Three such mutants have been characterized genetically and each was found to be associated with a different linkage group. Two of these linkage groups have not previously been described.
D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002470:Cell Survival; D002874:Chromosome Mapping; D004171:Diploidy; D008040:Genetic Linkage; D009153:Mutagens; D009154:Mutation; D009235:Myxomycetes / Q000187:drug effects / Q000254:growth & development*; D009604:Nitrosoguanidines; D013696:Temperature; D014466:Ultraviolet Rays
D009153:Mutagens; D009604:Nitrosoguanidines; D001973:Bromodeoxyuridine