Replication of coliphage M-13. II. Intracellular deoxyribonucleic acid forms associated with M-13 infection of mitomycin C-treated cells.
Intracellular deoxyribonucleic acid (DNA) forms associated with bacteriophage M-13 infection have been isolated and characterized. Escherichia coli HF4704 (F(+), hcr(-), thy(-)) cells were treated with mitomycin C to inhibit host-cell DNA synthesis and were then infected with phage M-13. This treatment permitted radioactive labeling of phage-specific DNA forms with (3)H-thymine. These labeled DNA components were characterized by sucrose density sedimentation and equilibrium density gradient centrifugation in neutral and ethidium bromide CsCl gradient. Two double-stranded circular forms were found with properties analogous to the replicative form I and replicative form II of phiX174. A third component, identified as single-stranded DNA, was isolated in some samples removed 45 min after phage synthesis was initiated.
D001205:Ascorbic Acid / Q000494:pharmacology; D002499:Centrifugation, Density Gradient; D003090:Coliphages / Q000254:growth & development*; D004279:DNA, Viral / Q000302:isolation & purification*; D004926:Escherichia coli / Q000187:drug effects*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008937:Mitomycins / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014779:Virus Replication
D004279:DNA, Viral; D008937:Mitomycins; D014316:Tritium; D001205:Ascorbic Acid; D013936:Thymidine