Determination of long-chain base in glycosphingolipids with fluorescamine.
A method is described for determining the long-chain base content of glycosphingolipids after acid hydrolysis, using the new reagent fluorescamine. The reaction is sensitive and can be used to characterize or measure glycosphingolipids in quantities routinely separated by thin-layer chromatography.
D000818:Animals; D001572:Benzofurans; D001921:Brain; D002417:Cattle; D002518:Ceramides / Q000032:analysis; D002855:Chromatography, Thin Layer; D005069:Evaluation Studies as Topic; D005690:Galactose / Q000032:analysis; D005732:Gangliosides / Q000032:analysis; D006801:Humans; D007202:Indicators and Reagents; D007668:Kidney; D008722:Methods; D008832:Microchemistry; D008970:Molecular Weight; D009439:Neuraminidase; D013050:Spectrometry, Fluorescence; D013107:Sphingolipids / Q000032:analysis*; D013141:Spiro Compounds; D014316:Tritium
D001572:Benzofurans; D002518:Ceramides; D005732:Gangliosides; D007202:Indicators and Reagents; D013107:Sphingolipids; D013141:Spiro Compounds; D014316:Tritium; D009439:Neuraminidase; D005690:Galactose