Application of paper chromatograms to the study of inducers of lambda bacteriophage in Escherichia coli.
A procedure has been developed whereby paper chromatograms of agents which induce lambda bacteriophage in Escherichia coli can be developed using bioautographs with a lysogenic test system. Well-defined plaque-forming zones are produced indicating the area on the paper chromatogram where the active inducing material can be located. A mixture of the bacteriophage-inducing antibiotic, mitomycin C, and the noninducing antibiotic, paromomycin, was resolved into its components on paper strips with an ethyl acetate-methanol solvent system. The location of both antibiotics could thus be readily observed. Antibacterial and inducing activities were found to be identical with a crude fermentation solid, NSC-B-158,791. The use of this procedure for resolution of multicomponent inducing activities in antibiotic beers and for characterization of active components which may be potential antitumor antibiotics is indicated.
D000970:Antineoplastic Agents / Q000032:analysis; D002854:Chromatography, Paper; D003090:Coliphages; D004926:Escherichia coli; D008937:Mitomycins / Q000032:analysis; D010303:Paromomycin / Q000032:analysis
D000970:Antineoplastic Agents; D008937:Mitomycins; D010303:Paromomycin