Cell division during inhibition of deoxyribonucleic acid synthesis in Escherichia coli.
When cultures of Escherichia coli B/r growing at various rates were exposed to ultraviolet light, mitomycin C, or nalidixic acid, deoxyribonucleic acid (DNA) synthesis stopped but cell division continued for at least 20 min. The chromosome configurations in the cells which divided were estimated by determining the rate of DNA synthesis during the division cycle. The cultures were pulse-labeled with (14)C-thymidine, and the amount of label incorporated into cells of different ages was found by measuring the radioactivity in cells born subsequent to the labeling period. The cells which divided in the absence of DNA synthesis were those which had completed a round of chromosome replication prior to the treatments. It was concluded that completion of a round of replication is a necessary and sufficient condition of DNA synthesis for cell division.
D002247:Carbon Isotopes; D002455:Cell Division / Q000187:drug effects*; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D008937:Mitomycins / Q000494:pharmacology; D009268:Nalidixic Acid / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014466:Ultraviolet Rays
D002247:Carbon Isotopes; D004269:DNA, Bacterial; D008937:Mitomycins; D014316:Tritium; D009268:Nalidixic Acid; D013936:Thymidine