title: Effect of nutrients on physiological properties of Clostridium botulinum type E.
abstract: Eight strains of Clostridium botulinum type E out of twelve tested showed good growth and normal cell morphology in a synthetic medium containing choline. Growth and toxin production by a representative strain was not influenced by repeated subculturing. In the chemically defined medium, acetylcholine, N,N-dimethylethanolamine, and lecithin could replace choline to get normal cell division and cell morphology of C. botulinum type E. Choline could not be replaced by ethanolamine, N-methylethanolamine, or betaine. A toxigenic strain of C. botulinum type E showed proteolytic, lipolytic, and lecithinase activity in complex media but not in a synthetic medium. On prolonged incubation in the high temperature range of growth, the toxicity of the culture filtrate decreased in a complex, but not in a synthetic medium. The implications of these findings are discussed.
mesh_terms: D000109:Acetylcholine / Q000378:metabolism; D001622:Betaine / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002794:Choline / Q000378:metabolism* / Q000494:pharmacology; D003013:Clostridium / Q000201:enzymology; D003014:Clostridium botulinum / Q000378:metabolism / Q000502:physiology*; D003470:Culture Media; D008049:Lipase / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism; D014118:Toxins, Biological / Q000096:biosynthesis
chemical_list: D003470:Culture Media; D010713:Phosphatidylcholines; D014118:Toxins, Biological; D001622:Betaine; D010740:Phospholipases; D008049:Lipase; D010447:Peptide Hydrolases; D002794:Choline; D000109:Acetylcholine