Shear and the melting of DNA: an especially sensitive portion of the E. coli genome.
The melting point of DNA is shown to be a function of shear stress. The higher the molecular weight of the DNA, the further its melting point is lowered by a given shear rate. During lysis of E. coli, a part of the DNA is especially shear sensitive, so that its melting curve in the presence of shear shows a low-melting region prior to the main transition. Lysis and dilution of the cell contents destroys the extra shear sensitivity, perhaps because the DNA dissociates from the cell membrane or from some other large subcellular structure. Such a structure would impart increased shear sensitivity to the associated region of the genome.
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004269:DNA, Bacterial; D004926:Escherichia coli; D008970:Molecular Weight; D011489:Protein Denaturation; D013696:Temperature
D004269:DNA, Bacterial