The control of sulphate reduction in Escherichia coli by O-acetyl-L-serine.
1. Extracts of Escherichia coli A.T.C.C. 9723 and K(12)703 contain serine transacetylase and O-acetylserine sulphhydrase. Synthesis of the latter enzyme is repressed by growth on l-cyst(e)ine and other sulphur compounds. 2. O-Acetyl-l-serine added to cells growing on glutathione or sulphate as source of sulphur induces the enzymes that catalyse (a) the activation of sulphate to adenosine 3'-phosphate 5'-sulphatophosphate (EC 2.7.7.4 and 2.7.1.25), (b) the reduction of adenosine 3'-phosphate 5'-sulphatophosphate to sulphite and (c) the reduction of sulphite to sulphide (EC 1.8.1.2). Hydrogen sulphide is liberated from cultures growing on sulphate as source of sulphur and in the presence of O-acetylserine. 3. The cysE mutants of E. coli K(12) lack serine transacetylase. Addition of O-acetylserine permits growth on sulphate as source of sulphur; at the same time the enzymes of sulphate reduction, previously absent, are synthesized. Such mutants have no detectable intracellular cyst(e)ine when starved of sulphur. 4. These results suggest that O-acetylserine is necessary for synthesizing the enzymes of sulphate reduction in E. coli. Its action does not appear to be by interference with the repressive control exerted over these enzymes by cyst(e)ine.
D000217:Acyltransferases / Q000378:metabolism; D003470:Culture Media; D004790:Enzyme Induction; D004794:Enzyme Repression; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D005827:Genetics, Microbial; D006836:Hydro-Lyases / Q000378:metabolism*; D009154:Mutation; D009713:Nucleotidyltransferases / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism*; D013460:Sulfur Isotopes; D014166:Transferases / Q000378:metabolism*
D003470:Culture Media; D013431:Sulfates; D013460:Sulfur Isotopes; D010088:Oxidoreductases; D014166:Transferases; D000217:Acyltransferases; D010770:Phosphotransferases; D009713:Nucleotidyltransferases; D006836:Hydro-Lyases