Base composition of deoxyribonucleic acid of the temperature-sensitive kanamycin-resistant R factor, Rts1.
The buoyant density of deoxyribonucleic acid (DNA) from the temperature-sensitive R factor, Rts1, was determined by CsCl density-gradient centrifugation. Rts1 was found to consist of a single species of DNA of density 1.705 g/cm(3), which corresponds to a base composition of 45% guanine plus cytosine. This value is distinct from the densities previously reported for other R factors, suggesting that Rts1 represents a new molecular class of R factors.
D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D003596:Cytosine / Q000032:analysis; D004269:DNA, Bacterial / Q000032:analysis*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli; D006147:Guanine / Q000032:analysis; D007612:Kanamycin / Q000494:pharmacology*; D011511:Proteus / Q000032:analysis*; D012706:Serratia marcescens / Q000032:analysis*; D013696:Temperature
D002712:Chlorides; D004269:DNA, Bacterial; D002586:Cesium; D007612:Kanamycin; D006147:Guanine; D003596:Cytosine