Peptide chain termination. VI. Purification and site of action of S.
PEPTIDE CHAIN TERMINATION IS A RESULT OF AT LEAST TWO EVENTS: terminator codon recognition and hydrolysis of peptidyl tRNA. A protein factor S, isolated from the supernatant of Escherichia coli B, stimulates fMet release. Factor S lowers the K(m) for terminator trinucleotides without altering the V(max) of release and therefore acts at terminator codon recognition. The S protein differs from initiation factors, elongation factor G, several forms of elongation factor T and release factors. The importance of the 2% Tu content in purified S is unresolved.
D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D005561:Formates / Q000378:metabolism; D005815:Genetic Code; D007700:Kinetics; D008715:Methionine; D009711:Nucleotides / Q000378:metabolism; D010452:Peptide Biosynthesis; D012343:RNA, Transfer / Q000378:metabolism; D014500:Uracil Nucleotides
D001426:Bacterial Proteins; D005561:Formates; D009711:Nucleotides; D014500:Uracil Nucleotides; D012343:RNA, Transfer; D008715:Methionine