Template activity of liver chromatin increased by in vivo administration of insulin.
The hypothesis that insulin may serve as a derepressor of genetic information in the liver of diabetic rats has been tested by comparing the template activity for RNA synthesis of chromatin from liver of insulin-treated diabetic rats to that of chromatin from liver of insulin-deficient diabetic rats. The template activity of the chromatins of insulin-treated diabetic rats is found to be 28 per cent greater than that of chromatin from liver of diabetic rats not treated with insulin. Time course studies show that the template activity of liver chromatin of rats injected with a single dose of insulin reaches a peak at two hours, which is some hours before the appearance of a typical insulin-induced liver enzyme, glucokinase. We conclude that insulin derepresses genetic material of the diabetic liver genome that is repressed in the absence of insulin.
D000818:Animals; D002875:Chromosomes / Q000187:drug effects / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004794:Enzyme Repression; D004926:Escherichia coli / Q000201:enzymology; D005941:Glucokinase / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D012313:RNA / Q000096:biosynthesis; D012316:RNA Nucleotidyltransferases / Q000378:metabolism; D051381:Rats; D013268:Stimulation, Chemical; D013698:Templates, Genetic
D007328:Insulin; D012313:RNA; D005941:Glucokinase; D012316:RNA Nucleotidyltransferases