Colorimetric assay for lysine decarboxylase in Escherichia coli.
A new assay is described for lysine decarboxylase. It is rapid and reproducible in assaying large numbers of samples, a situation in which earlier methods were less convenient. The new method is valuable in the study of peptide fractions and amino acid mixtures which stimulate induction of lysine decarboxylase. It may be useful for work on enzyme structure and modification, genetics, and kinetics.
D000596:Amino Acids / Q000032:analysis; D002262:Carboxy-Lyases / Q000032:analysis*; D004796:Clinical Enzyme Tests; D003124:Colorimetry; D004790:Enzyme Induction; D004926:Escherichia coli / Q000201:enzymology*; D007700:Kinetics; D008365:Manometry; D008722:Methods; D008967:Molecular Biology; D010455:Peptides / Q000032:analysis
D000596:Amino Acids; D010455:Peptides; D002262:Carboxy-Lyases