5S RNA synthesized by Escherichia coli in presence of chloramphenicol: different 5'-terminal sequences.
Escherichia coli cells, grown in the presence of chloramphenicol, synthesize a low molecular weight RNA (CM-5S RNA) not bound to ribosomes which is similar to ribosomal 5S RNA. Oligonucleotide patterns derived from ribonuclease digests of 5S RNA and of CM-5S RNA are indistinguishable except that the 5'-terminal oligonucleotides differ. Whereas the nucleotide sequence of the 5'-terminus of normal 5S RNA is (p)U(p)G-, there are three alternate sequences of the 5'-terminus of CM-5S RNA: (p)U(p)U(p)G-, (p)U(p)U(p)U(p)G-, and (p)A(p)U(p)U(p)U(p)G-.
D001345:Autoradiography; D002701:Chloramphenicol / Q000494:pharmacology*; D002854:Chromatography, Paper; D004586:Electrophoresis; D004926:Escherichia coli / Q000187:drug effects*; D008970:Molecular Weight; D009711:Nucleotides / Q000032:analysis*; D011119:Polynucleotides / Q000032:analysis; D012329:RNA, Bacterial / Q000096:biosynthesis*; D012270:Ribosomes
D009711:Nucleotides; D011119:Polynucleotides; D012329:RNA, Bacterial; D002701:Chloramphenicol