Modified microbiological assay for rapid estimation of antibiotic concentrations in human sera.
Antibiotic concentrations in human sera were estimated in 5 to 6 hr by a modified microbiological assay. By using Staphylococcus aureus and Streptococcus pyogenes as the assay organisms, the seeded assay plates were preincubated for 2 to 6 hr and then were stored at 4 C until used for assay. Paper discs saturated with the specimen were placed on the preincubated assay plates with reference discs saturated with known concentrations of antibiotic. After 5 to 6 hr of incubation, zones of antibacterial activity were measured and compared with a standard curve for estimation of antibiotic concentration. Results from this rapid assay method compared favorably with those from the commonly used 24-hr assay.
D000900:Anti-Bacterial Agents / Q000097:blood*; D001681:Biological Assay; D002509:Cephaloridine / Q000008:administration & dosage / Q000097:blood; D005839:Gentamicins / Q000008:administration & dosage / Q000097:blood; D006801:Humans; D007612:Kanamycin / Q000008:administration & dosage / Q000097:blood; D008722:Methods; D013210:Staphylococcus / Q000187:drug effects; D013297:Streptococcus pyogenes / Q000187:drug effects; D013307:Streptomycin / Q000008:administration & dosage / Q000097:blood; D014640:Vancomycin / Q000008:administration & dosage / Q000097:blood
D000900:Anti-Bacterial Agents; D005839:Gentamicins; D007612:Kanamycin; D014640:Vancomycin; D002509:Cephaloridine; D013307:Streptomycin