title: Characteristics of a new sterol-nonrequiring Mycoplasma.
abstract: Two Mycoplasma strains recovered from tissue culture environments were found to grow in complex media devoid of serum or serum fractions containing cholesterol and in a cholesterol-free synthetic medium. Neither strain was capable of synthesizing pigmented carotenoids, although these compounds are present in, and characteristic of, other sterol-nonrequiring mycoplasmas. Serological tests and an analysis of their cell protein patterns obtained by gel electrophoresis indicated that the isolates were similar to each other but distinct from other sterol-nonrequiring serotypes, Mycoplasma laidlawii and M. granularum, as well as from sterol-requiring species. The existence of Mycoplasma other than M. laidlawii and M. granularum without sterol requirements suggested the need for some taxonomic changes in this group of organisms.
mesh_terms: D000085:Acetates / Q000378:metabolism; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D002247:Carbon Isotopes; D002338:Carotenoids / Q000096:biosynthesis; D002784:Cholesterol / Q000378:metabolism* / Q000494:pharmacology; D003470:Culture Media; D046508:Culture Techniques; D004589:Electrophoresis, Disc; D005455:Fluorescent Antibody Technique; D007106:Immune Sera / Q000494:pharmacology; D007942:Leukemia, Experimental; D050356:Lipid Metabolism; D051379:Mice; D009174:Mycoplasma / Q000032:analysis / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism; D009995:Osmosis; D012703:Serotyping; D013501:Surface-Active Agents / Q000494:pharmacology
chemical_list: D000085:Acetates; D001426:Bacterial Proteins; D002247:Carbon Isotopes; D003470:Culture Media; D007106:Immune Sera; D013501:Surface-Active Agents; D002338:Carotenoids; D002784:Cholesterol