Intracellular inactivation of bacteriophage T4 early deoxyribonucleic acid.
As previously shown, a small amount of polynucleotide material is added to parental T4 deoxyribonucleic acid (DNA) molecules within the first 5 min of infection. I have asked whether this process is essential for phage replication. Two approaches-one involving decay of (32)P incorporated into this "early DNA" and the other involving photoinactivation of bromodeoxyuridine-containing early DNA-indicate that it is.
D001973:Bromodeoxyuridine; D002499:Centrifugation, Density Gradient; D003090:Coliphages / Q000254:growth & development* / Q000378:metabolism; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D004926:Escherichia coli / Q000378:metabolism; D005827:Genetics, Microbial; D010710:Phosphates; D010759:Phosphorus Isotopes; D013395:Sucrose; D013941:Thymine / Q000378:metabolism; D014466:Ultraviolet Rays; D014779:Virus Replication
D004279:DNA, Viral; D010710:Phosphates; D010759:Phosphorus Isotopes; D013395:Sucrose; D001973:Bromodeoxyuridine; D013941:Thymine