Simple method for rapid determination of antibiotic blood levels.
A simple method for the rapid determination of antibiotic blood levels is described. Results are readily obtainable within 4 to 6 hr. The reported procedure is based upon determination of the highest dilution of a patient's serum capable of inhibiting dextrose fermentation by a standardized inoculum of a staphylococcus strain or of an Escherichia coli strain, when colymycin or polymyxin are involved, as compared to previously determined endpoints in this regard of defined concentrations of the different antibiotics dissolved in normal human serum. Excellent correlation was observed with 36 serum specimens that contained different antimicrobial agents in varied concentrations when simultaneously assayed by the standard method and the subject procedure of this report.
D000900:Anti-Bacterial Agents / Q000097:blood*; D001431:Bacteriological Techniques; D004926:Escherichia coli / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D008722:Methods; D013210:Staphylococcus / Q000378:metabolism; D013997:Time Factors
D000900:Anti-Bacterial Agents; D005947:Glucose