Control of uridine diphosphate-glucose dehydrogenase synthesis and uridine diphosphate-glucuronic acid accumulation by a regulator gene mutation in Escherichia coli K-12.
Uridine diphosphate (UDP)-glucose dehydrogenase, the enzyme that converts UDP-glucose to UDP-glucuronic acid, was derepressed in a mucoid (capR9) strain of Escherichia coli K-12 and repressed in a nonmucoid (capR(+)) strain. A nonmucoid mutant (strain MC 152; capR9 non-2) derived from the mucoid strain accumulated large quantities of nucleotides. Among these nucleotides, UDP-glucuronic acid was identified as well as guanosine triphosphate and an adenosine diphosphate-sugar. UDP-glucose dehydrogenase was still derepressed in strain MC 152. When the nonmucoid mutant was transduced to the wild-type state for this regulator gene (capR(+)), the transductant was found to accumulate less total nucleotides, and the accumulation of UDP-glucuronic acid was abolished. UDP-glucose dehydrogenase was repressed in the capR(+)non-2 strain but not to the same extent that it was in the capR(+) strain.
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism; D002854:Chromatography, Paper; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D005809:Genes, Regulator; D005838:Genotype; D005947:Glucose / Q000378:metabolism; D005965:Glucuronates / Q000032:analysis / Q000378:metabolism*; D009154:Mutation; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D013053:Spectrophotometry; D014161:Transduction, Genetic; D014500:Uracil Nucleotides / Q000032:analysis / Q000378:metabolism*
D005965:Glucuronates; D011135:Polysaccharides, Bacterial; D014500:Uracil Nucleotides; D000429:Alcohol Oxidoreductases; D005947:Glucose