title: The pepsins of normal human gastric juice.
abstract: 1. The frequency of occurrence, under defined conditions, of the different human pepsins in the gastric juices of 50 normal subjects was investigated by agar-gel electrophoresis. 2. From a total of eight proteolytic zones located in the zymograms, no significant differences of occurrence existed between the sexes, or between subjects with or without gastric symptoms. 3. Two zones, numbered 3 and 5, occurred in all normal gastric juices. Zone 3 always exhibited the greatest proteolytic activity, then zone 5. The remaining enzymic zones were less well-marked and occurred less frequently. 4. A minor zone, 3a, was demonstrated within zone 3. The corresponding pepsin, 3a, has a mobility towards the anode 6-7% greater than has pepsin 3. 5. Of the eight zones, 1,2,3,3a and 5, at least, represent unique pepsins.
mesh_terms: D000362:Agar; D002852:Chromatography, Ion Exchange; D004586:Electrophoresis; D005260:Female; D005750:Gastric Juice / Q000201:enzymology*; D005782:Gels; D006801:Humans; D008297:Male; D010434:Pepsin A / Q000032:analysis*; D012737:Sex Factors; D013272:Stomach Diseases / Q000201:enzymology
chemical_list: D005782:Gels; D000362:Agar; D010434:Pepsin A