Use of dimethyl suberimidate, a cross-linking reagent, in studying the subunit structure of oligomeric proteins.
Amidination of aldolase, glyceraldehyde-3-phosphate dehydrogenase, tryptophan synthetase B protein, L-arabinose isomerase, and the catalytic subunit of E. coli aspartate transcarbamylase with the bifunctional reagent dimethyl suberimidate produces cross-linked proteins, with reaction predominating within oligomers. Disc electrophoresis of a modified protein on polyacrylamide gel in the presence of sodium dodecyl sulfate resolves a set of species with molecular weights equal to integral multiples of the protomer molecular weight. For oligomers composed of identical protomers, the number of principal species observed is identical to the number of protomers in the oligomer. Application of the method to two proteins composed of dissimilar protomers, native aspartate transcarbamylase and tryptophan synthetase alpha(2)beta(2) complex of E. coli, revealed differences in the reactivities of the different kinds of protomer within each oligomer.
D000578:Amidines; D001089:Arabinose; D001224:Aspartic Acid; D004589:Electrophoresis, Disc; D004926:Escherichia coli / Q000201:enzymology; D004952:Esters; D005634:Fructose-Bisphosphate Aldolase / Q000032:analysis; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000032:analysis; D007094:Imides; D007202:Indicators and Reagents; D007535:Isomerases / Q000032:analysis; D008025:Ligases / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D013501:Surface-Active Agents; D014166:Transferases / Q000032:analysis; D014364:Tryptophan; D014461:Ultracentrifugation
D000578:Amidines; D004952:Esters; D007094:Imides; D007202:Indicators and Reagents; D011506:Proteins; D013501:Surface-Active Agents; D001224:Aspartic Acid; D014364:Tryptophan; D001089:Arabinose; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D014166:Transferases; D005634:Fructose-Bisphosphate Aldolase; D007535:Isomerases; D008025:Ligases