title: Loss of lysis inhibition in filamentous Escherichia coli infected with wild-type bacteriophage T4.
abstract: The plaque enlargement of wild-type T4 bacteriophage observed when assayed in the presence of low concentrations of mitomycin C or after exposure to very low doses of ultraviolet light was studied by using solid as well as liquid culture media. It was found that the filamentous cell formed by the treatment with the agents is responsible for the phenomenon. The filamentous cell was also shown to be characterized not only by the loss of capacity of lysis inhibition but also by a shortening of the latent period. No difference in cellular rigidity could be seen between the filamentous cell and normal cell as far as the analysis from the outside of the cell was concerned, whereas the former cell was shown to be more readily susceptible to phage-induced lysozyme from the inside of the cell. A possible change in the membrane of the filamentous cell and a possible mechanism for lysis inhibition are discussed.
mesh_terms: D001433:Bacteriolysis; D002473:Cell Wall / Q000187:drug effects; D003090:Coliphages / Q000201:enzymology; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D008937:Mitomycins / Q000494:pharmacology; D009113:Muramidase / Q000494:pharmacology
chemical_list: D008937:Mitomycins; D009113:Muramidase