Segregation into and replication of plasmid deoxyribonucleic acid in chromosomeless segregants of Escherichia coli.
The progeny cells of Escherichia coli strain P678-54, which normally do not contain deoxyribonucleic acid (DNA), were found to carry DNA when the parental cell carried colicin factor E1 (Col E1). The DNA found in the normally DNA-less segregants was shown to be Col E1 DNA, which is present primarily as a covalently closed circular molecule that can undergo more than one complete cycle of replication.
D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D002876:Chromosomes, Bacterial; D003087:Colicins / Q000096:biosynthesis; D003090:Coliphages; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D004926:Escherichia coli / Q000254:growth & development* / Q000378:metabolism; D005827:Genetics, Microbial; D009154:Mutation; D009691:Nucleic Acid Denaturation; D010179:Pancreas / Q000201:enzymology; D010759:Phosphorus Isotopes; D013395:Sucrose; D014316:Tritium
D002712:Chlorides; D003087:Colicins; D004269:DNA, Bacterial; D010759:Phosphorus Isotopes; D014316:Tritium; D002586:Cesium; D013395:Sucrose; D003851:Deoxyribonucleases