title: A factor that stimulates RNA synthesis by purified RNA polymerase.
abstract: A protein factor, partially purified from E. coli, can stimulate some 30- to 50-fold the transcription of DNA by purified RNA polymerase. Evidence is presented suggesting that this stimulatory factor (designated M factor) exerts its effect before the RNA synthesis is initiated. The M factor is prepared from a crude ribosome fraction, but it does not require the presence of ribosomes for full activity.
mesh_terms: D002499:Centrifugation, Density Gradient; D003090:Coliphages; D004926:Escherichia coli; D007700:Kinetics; D008722:Methods; D012313:RNA / Q000096:biosynthesis*; D012316:RNA Nucleotidyltransferases; D012270:Ribosomes; D013698:Templates, Genetic
chemical_list: D012313:RNA; D012316:RNA Nucleotidyltransferases