Absence of adenine-rich ribonucleic acid from purified infectious reovirus 3.
The basic properties of released and cell-associated reovirus are the same. Both contained as their total nucleic acid complement only double-stranded ribonucleic acid (RNA) with an adenine content of 28%. Preparations of purified cell-associated virus, but not released virus, contained adenine-rich RNA which could be separated from the virus with little or no loss of infectivity. These adenine-rich ribonucleic acids were present in the virus preparations either as free RNA or associated with some structures of molecular weight less than 25 x 10(6) daltons. In contrast to our previous report, double-stranded reovirus RNA possessed little or no template activity for the Escherichia coli deoxyribonucleic acid and RNA polymerases.
D000225:Adenine / Q000032:analysis*; D000595:Amino Acid Sequence; D002247:Carbon Isotopes; D004269:DNA, Bacterial; D004926:Escherichia coli; D007739:L Cells / Q000032:analysis; D008967:Molecular Biology; D010759:Phosphorus Isotopes; D012316:RNA Nucleotidyltransferases; D012367:RNA, Viral / Q000032:analysis*; D012087:Reoviridae / Q000032:analysis* / Q000302:isolation & purification; D014316:Tritium; D014779:Virus Replication
D002247:Carbon Isotopes; D004269:DNA, Bacterial; D010759:Phosphorus Isotopes; D012367:RNA, Viral; D014316:Tritium; D012316:RNA Nucleotidyltransferases; D000225:Adenine