title: Codon recognition by enzymatically mischarged valine transfer ribonucleic acid.
abstract: Direct evidence for the adaptor hypothesis has been obtained by examining the codon recognition of a purified Escherichia coli valine transfer ribonucleic acid which was enzymatically mischarged with phenylalanine labeled with carbn-14 by reaction with purified phenylalanyl-transfer ribonucleic acid synthetase from Neurospora crassa. The mischarged transfer ribonucleic acid recognized the valine codons but failed to recognize the phenylalanine codon when tested in trinucleotide-directed ribosomal binding assay.
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis; D002247:Carbon Isotopes; D004926:Escherichia coli / Q000378:metabolism; D005815:Genetic Code; D009491:Neurospora / Q000201:enzymology; D010649:Phenylalanine; D012329:RNA, Bacterial; D012343:RNA, Transfer; D014633:Valine
chemical_list: D001426:Bacterial Proteins; D002247:Carbon Isotopes; D012329:RNA, Bacterial; D010649:Phenylalanine; D012343:RNA, Transfer; D014633:Valine