title: Salmonella abony-Salmonella typhimurium recombinant nonvirulent for the mouse.
abstract: A previous genetic investigation involving a mouse-nonvirulent Salmonella abony donor (high frequency of recombination) and a virulent S. typhimurium recipient indicated that two unlinked "low-virulence" loci determined nonvirulence. A nonvirulent recombinant was analyzed to determine the basis for its nonvirulence. The recombinant was smooth (like the parental strains) and prototrophic. The doubling time in mouse serum of the recombinant and the S. abony parent (both streptomycin-resistant) was longer than that of the wild-type streptomycin-sensitive ancestor of the S. typhimurium recipient. The virulent recipient also grew poorly in serum. However, the nonvirulence of the recombinant was probably not due to its inheritance of the streptomycin-resistance allele from the donor, because other recombinants were streptomycin-resistant but still virulent. Unlike the nonvirulent S. abony (but like the S. typhimurium), the recombinant was insusceptible to rapid intravenous clearance in normal mice. It therefore appears that neither of the "low-virulence" loci determine diminished virulence by enhancing phagocytosis. Clearance of the recombinant was enhanced by opsonization with immune serum. Counts of viable bacteria in the blood, liver, and spleen of normal mice after intravenous challenge showed that the recombinant, like the S. abony donor, failed to proliferate in the tissues, whereas the virulent S. typhimurium did so markedly. It is concluded that the nonproliferation of the recombinant was determined by one or both of the "low-virulence" loci from the nonvirulent S. abony donor.
mesh_terms: D000818:Animals; D000906:Antibodies; D001769:Blood / Q000382:microbiology; D001770:Blood Bactericidal Activity; D004352:Drug Resistance, Microbial; D005827:Genetics, Microbial; D008099:Liver / Q000382:microbiology; D051379:Mice; D010587:Phagocytosis; D011995:Recombination, Genetic; D012475:Salmonella / Q000187:drug effects / Q000472:pathogenicity; D012486:Salmonella typhimurium; D013154:Spleen / Q000382:microbiology; D013307:Streptomycin / Q000494:pharmacology; D014774:Virulence
chemical_list: D000906:Antibodies; D013307:Streptomycin