Dye-sensitized photooxidation of the Escherichia coli ribosome.
Chemical modification of ribosomes from Escherichia coli by oxidation in the presence of selected photosensitizing dyes causes a rapid loss of their amino acid-incorporating ability. By comparing the efficiency of dyes that sensitize the photooxidation of either guanine in ribonucleic acid or amino acid residues in proteins, inactivation of one or more functional ribosomal proteins is inferred.
D000166:Acridines / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000096:biosynthesis*; D002247:Carbon Isotopes; D004396:Coloring Agents / Q000494:pharmacology*; D004247:DNA / Q000528:radiation effects; D004926:Escherichia coli / Q000166:cytology / Q000378:metabolism*; D008027:Light; D008239:Lysine / Q000378:metabolism; D008751:Methylene Blue / Q000494:pharmacology*; D012367:RNA, Viral / Q000528:radiation effects; D011830:Radiation Effects; D012270:Ribosomes / Q000187:drug effects* / Q000378:metabolism* / Q000528:radiation effects; D012395:Rose Bengal / Q000494:pharmacology*
D000166:Acridines; D001426:Bacterial Proteins; D002247:Carbon Isotopes; D004396:Coloring Agents; D012367:RNA, Viral; D012395:Rose Bengal; D004247:DNA; D008239:Lysine; D008751:Methylene Blue