title: Applicability of aeration and delayed addition of selenite to the isolation of salmonellae.
abstract: The effect of aeration combined with the delayed addition of selenite on the lag period of several strains of salmonellae and other enterobacteria is reported. A procedure has been developed involving shaking of the sample in a basal medium for 4 hr at 37 C, adding selenite and cystine, and continuing shaking for 20 hr. Confirmation by selective plating, biochemical tests, and serology gave results comparable to the standard lactose pre-enrichment method with the saving of 24 hr and elimination of one set of media. Confirmation by fluorescent-antibody tests showed that fewer positive fluorescent stains were obtained from the aerated procedure than from the lactose pre-enrichment procedure. Precautions in the application of this procedure are discussed.
mesh_terms: D000388:Air; D001431:Bacteriological Techniques; D003470:Culture Media; D003553:Cystine; D004755:Enterobacteriaceae / Q000302:isolation & purification; D004926:Escherichia coli / Q000302:isolation & purification; D005516:Food Microbiology; D012475:Salmonella / Q000254:growth & development / Q000302:isolation & purification*; D012486:Salmonella typhimurium / Q000254:growth & development / Q000302:isolation & purification; D012643:Selenium
chemical_list: D003470:Culture Media; D003553:Cystine; D012643:Selenium