title: Infection of tobacco mesophyll protoplasts by tobacco mosaic virus.
abstract: It was proved that the protoplasts prepared from mesophyll of Nicotiana tabacum are infected by tobacco mosaic virus. The infection occurred when purified tobacco mosaic virus particles were added to a protoplast suspension in the presence of poly-L-ornithine. The virus multiplied in these protoplasts to a level of 10(6) virus particles per infected protoplast during 24 hours of incubation. The efficiency of infection was remarkably high, exceeding that by mechanical inoculation of tobacco leaves.
mesh_terms: D002701:Chloramphenicol / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D003588:Cytopathogenic Effect, Viral; D003609:Dactinomycin / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D010455:Peptides / Q000494:pharmacology; D010947:Plants, Toxic; D011523:Protoplasts / Q000187:drug effects / Q000382:microbiology*; D013889:Thiouracil / Q000494:pharmacology; D014026:Nicotiana; D014027:Tobacco Mosaic Virus / Q000472:pathogenicity*
chemical_list: D010455:Peptides; D003609:Dactinomycin; D013889:Thiouracil; D002701:Chloramphenicol; D003513:Cycloheximide