title: Mutants of Escherichia coli K-12 with an altered glutamyl-transfer ribonucleic acid synthetase.
abstract: Three streptomycin-suppressible lethal mutants of Escherichia coli K-12 have been shown to possess structurally altered glutamyl-transfer ribonucleic acid (tRNA) synthetases. Each mutant synthetase displays a K(m) value for glutamate which is 10-fold higher than the parental value, and the mutations reside in two widely separate loci on the genetic map. Mixing of the mutant extracts in pairs gave no indication of in vitro complementation. All three enzymes charge the minor tRNA(glu) fraction identically, but one (EM 120) charges the major fraction at a twofold lower rate than do the other two (EM 102 and EM 111). Possible explanations for the existence of the two synthetase loci are presented.
mesh_terms: D002247:Carbon Isotopes; D002482:Cellulose; D002874:Chromosome Mapping; D004926:Escherichia coli / Q000201:enzymology* / Q000302:isolation & purification / Q000378:metabolism; D005021:Ethylamines; D005816:Genetic Complementation Test; D005827:Genetics, Microbial; D005971:Glutamates / Q000378:metabolism*; D006358:Hot Temperature; D008025:Ligases / Q000378:metabolism*; D009154:Mutation; D012343:RNA, Transfer; D013307:Streptomycin / Q000378:metabolism
chemical_list: D002247:Carbon Isotopes; D005021:Ethylamines; D005971:Glutamates; D002482:Cellulose; D012343:RNA, Transfer; D008025:Ligases; D013307:Streptomycin