Production of the monamycins, novel depsipeptide antibiotics.
Methods are described for the production of the monamycins by Streptomyces jamaicensis in shake flasks and jar fermentors. The effects on the fermentation of variations in pH, temperature, medium composition, volume of inoculum, and strain of the organism are discussed. The methods employed for the extraction and for the microbiological assay of the antibiotics are outlined.
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001681:Biological Assay; D002855:Chromatography, Thin Layer; D003470:Culture Media; D015643:Erythromycin Ethylsuccinate / Q000096:biosynthesis / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D008722:Methods; D009154:Mutation; D013302:Streptomyces / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D014466:Ultraviolet Rays
D000900:Anti-Bacterial Agents; D003470:Culture Media; D015643:Erythromycin Ethylsuccinate