Isolation of adenyl cyclase from Escherichia coli.
We have found a soluble cyclase, using for assay radioactively marked ATP as precursor. The reaction product was isolated by thin-layer chromatography and identified by specific degradation. After homogenization, part of the activity remained in the particulate fraction but could be easily extracted. The cyclase was concentrated 100-fold by conventional methods. The enzyme has a Mg(++) requirement and is inhibited by fluoride and inorganic pyrophosphate.
D000227:Adenine Nucleotides; D000262:Adenylyl Cyclases / Q000302:isolation & purification; D002855:Chromatography, Thin Layer; D004798:Enzymes / Q000302:isolation & purification*; D004926:Escherichia coli / Q000201:enzymology*; D008274:Magnesium; D008722:Methods
D000227:Adenine Nucleotides; D004798:Enzymes; D000262:Adenylyl Cyclases; D008274:Magnesium