Temperature-sensitive mutants of respiratory syncytial virus.
Four conditional-lethal temperature-sensitive mutants of RS virus were detected among the progeny of 454 plaques derived from virus grown in the presence of 10(-4)m 5-fluorouridine. These mutants were stable (reversion frequency, 10(-5.0) or less and failed to produce plaques at 38 or 39 C. Plaquing efficiency was depressed 100-fold or more at 37 C. Variable suppression of growth at the restrictive temperature of 39 C was observed, ranging from 16-fold to complete suppression. The temperature-sensitive defect of three of the mutants appeared to affect functions which were expressed late in the replicative cycle. One of the mutants produced atypical nonsyncytial plaques.
D000941:Antigens / Q000032:analysis; D003168:Complement Fixation Tests; D046508:Culture Techniques; D003588:Cytopathogenic Effect, Viral; D005455:Fluorescent Antibody Technique; D005827:Genetics, Microbial; D006367:HeLa Cells; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation; D009603:Nitroso Compounds / Q000494:pharmacology; D012136:Respiratory Syncytial Viruses / Q000187:drug effects; D013696:Temperature; D014529:Uridine / Q000494:pharmacology
D000941:Antigens; D009153:Mutagens; D009603:Nitroso Compounds; D014529:Uridine