title: Abortive infection of Shigella dysenteriae P2 by T2 bacteriophage.
abstract: We have investigated some of the biochemical events that accompany the abortive infection by T2 of Shigella dysenteriae lysogenized with the temperate phage P2. After infection with T2, protein and RNA synthesis continued for 3 to 5 min. The virus-induced enzyme, deoxycytidylate hydroxymethylase was produced in reduced amounts (15% of normal), and the extent of deoxyribonucleic acid (DNA) synthesis was 0.1% of that found with a nonlysogenic strain. Measurements of the production of acid-soluble fragments and sedimentation analyses failed to detect enzymatic degradation of the infecting viral DNA which could be specifically related to the presence of the prophage P2. Each interaction between T2 and a bacterium resulted in the death of the cell. This observation is consistent with results obtained with other types of bacteria which show that only when a nucleolytic attack occurs on T2 DNA does the cell have an increased capacity to survive after adsorption of T2.
mesh_terms: D001435:Bacteriophages; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D003090:Coliphages; D004279:DNA, Viral / Q000096:biosynthesis / Q000378:metabolism; D004790:Enzyme Induction; D007532:Isoleucine / Q000378:metabolism; D008242:Lysogeny; D012762:Shigella dysenteriae; D014166:Transferases / Q000378:metabolism; D014316:Tritium; D014498:Uracil / Q000378:metabolism; D014762:Viral Interference; D014764:Viral Proteins / Q000096:biosynthesis
chemical_list: D002247:Carbon Isotopes; D004279:DNA, Viral; D014764:Viral Proteins; D007532:Isoleucine; D014316:Tritium; D014498:Uracil; D014166:Transferases