Accelerated plaque formation by fowlpox virus in the presence of chymotrypsin.
Chymotrypsin enhanced fowlpox virus plaque formation in chick embryo cell cultures. A simplified plaque assay for fowlpox virus is described. Plaques were produced in 3 days when chymotrypsin was included in a serum-free fluid overlay. Plaques were also produced in 5 to 6 days under an agar overlay when a medium containing fetal calf serum was employed. Kinetics of plaque formation were also studied, and it was shown that fowlpox virus plaque diameters grow at a linear rate.
D000362:Agar; D000818:Animals; D000917:Antibody Formation; D002642:Chick Embryo; D002918:Chymotrypsin / Q000494:pharmacology*; D003470:Culture Media; D046508:Culture Techniques; D006462:Hemolytic Plaque Technique; D007700:Kinetics; D008722:Methods; D011212:Poxviridae / Q000254:growth & development / Q000276:immunology*; D014779:Virus Replication
D003470:Culture Media; D000362:Agar; D002918:Chymotrypsin