Variation in the N-terminal sequence of heavy chains of immunoglobulin G from rabbits of different allotype.
The sequences of the N-terminal peptides prepared by Pronase digestion of the heavy chain of rabbit immunoglobulin G of allotype Aa1, Aa2 and Aa3 were determined and were shown to be related to the allotype. An N-terminal fragment of about 34 residues was also prepared from the allotype heavy chains, by cleavage with cyanogen bromide; the yield varied with the allotype. The sequences of the cyanogen bromide fragments from the Aa1 and Aa3 heavy chains contain allotype-related variations similar to those found in the N-terminal Pronase peptides, and these sequences are thought to be representative of the whole heavy-chain populations. There is about 60% homology between the two sequences, and superimposed on the differences between them there are a number of positions within each sequence at which at least two amino acids are present.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001322:Autoanalysis; D001966:Bromine; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D002918:Chymotrypsin; D003486:Cyanides; D004586:Electrophoresis; D014644:Genetic Variation; D005838:Genotype; D007074:Immunoglobulin G / Q000032:analysis; D010209:Paper; D010434:Pepsin A; D010447:Peptide Hydrolases; D010455:Peptides / Q000032:analysis; D011817:Rabbits; D014357:Trypsin
D000596:Amino Acids; D003486:Cyanides; D007074:Immunoglobulin G; D010455:Peptides; D010447:Peptide Hydrolases; D002918:Chymotrypsin; D014357:Trypsin; D010434:Pepsin A; D001966:Bromine