Cell sorting: automated separation of mammalian cells as a function of intracellular fluorescence.
A system for high-speed sorting of fluorescent cells was able to sort mouse spleen cells from Chinese hamster ovarian cells after development of fluorochromasia. Highly fluorescent fractions separated after similar treatment from mouse spleen cells immunized to sheep erythrocytes were enriched in antibody-producing cells by factors of 4 to 10.
D000818:Animals; D001331:Automation; D003585:Cell Biology; D006224:Cricetinae; D046508:Culture Techniques; D005260:Female; D005452:Fluoresceins / Q000494:pharmacology; D005453:Fluorescence; D005455:Fluorescent Antibody Technique; D008722:Methods; D051379:Mice; D009374:Neoplasms, Experimental; D010051:Ovarian Neoplasms; D010053:Ovary / Q000187:drug effects; D013154:Spleen / Q000187:drug effects
D005452:Fluoresceins