Studies relating to the mode of action of methotrexate. 3. Inhibition of thymidylate synthetase in tissue culture cells and in cell-free systems.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology; D002474:Cell-Free System / Q000187:drug effects; D002852:Chromatography, Ion Exchange; D006224:Cricetinae; D046508:Culture Techniques; D004247:DNA / Q000378:metabolism; D003857:Deoxyuridine / Q000378:metabolism; D004622:Embryo, Mammalian / Q000187:drug effects; D004926:Escherichia coli / Q000201:enzymology; D005492:Folic Acid / Q000378:metabolism; D006651:Histocytochemistry; D007700:Kinetics; D007739:L Cells; D002955:Leucovorin / Q000494:pharmacology; D008727:Methotrexate / Q000494:pharmacology*; D051379:Mice; D009711:Nucleotides / Q000096:biosynthesis / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014166:Transferases / Q000037:antagonists & inhibitors*; D014316:Tritium
D009711:Nucleotides; D014316:Tritium; D012313:RNA; D004247:DNA; D005492:Folic Acid; D014166:Transferases; D002955:Leucovorin; D013936:Thymidine; D003857:Deoxyuridine; D008727:Methotrexate