Inhibitory effect of penicillin on proline synthesis in Escherichia coli.
The production of glutamic gamma-semialdehyde, an intermediate in the synthesis of proline, was inhibited in Escherichia coli by physiological concentrations of penicillin. Sucrose (0.6 m) and sodium chloride (0.1 m) prevented penicillin inhibition of glutamic gamma-semialdehyde synthesis. Cells which were in the stationary phase, or which had been permitted to metabolize without growth, were insensitive to the effects of penicillin on glutamic gamma-semialdehyde synthesis.
D000447:Aldehydes / Q000096:biosynthesis*; D001665:Binding Sites; D002247:Carbon Isotopes; D002498:Centrifugation; D003470:Culture Media; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D005971:Glutamates / Q000096:biosynthesis*; D010400:Penicillin G / Q000494:pharmacology; D011392:Proline / Q000096:biosynthesis*; D012965:Sodium Chloride / Q000037:antagonists & inhibitors; D013395:Sucrose / Q000037:antagonists & inhibitors; D013997:Time Factors
D000447:Aldehydes; D002247:Carbon Isotopes; D003470:Culture Media; D005971:Glutamates; D012965:Sodium Chloride; D013395:Sucrose; D011392:Proline; D010400:Penicillin G