Endonuclease II of E. coli. I. Isolation and purification.
The isolation and purification of a new endonuclease of E. coli is described. This enzyme degrades alkylated DNA as assayed by a technique that requires double-strand scission. The enzyme also makes a limited number of single-strand breaks in native nonalkylated DNA.
D000478:Alkylation; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D004269:DNA, Bacterial; D003851:Deoxyribonucleases / Q000302:isolation & purification*; D004589:Electrophoresis, Disc; D004926:Escherichia coli / Q000201:enzymology*; D008722:Methods
D004269:DNA, Bacterial; D003851:Deoxyribonucleases