Accelerated immunofluorescence procedure for the detection of Salmonella in foods and animal by-products.
An accelerated, direct immunofluorescent-antibody procedure was developed for the detection of Salmonella in food products. This method includes pre-enrichment and selective enrichment but eliminates many of the washing and smear treatments present in existing methods. Commercially available fluorescein-conjugated somatic antiserum was used in comparing this method with conventional culture, biochemical, and serological procedures. The 894 samples tested represented 39 different products. The fluorescent-antibody procedure detected Salmonella in 216 test samples as compared to 205 positives recovered by using the standard culture procedures. In no instance did the fluorescent-antibody procedure fail to detect a Salmonella positive which had been detected by the standard procedure. With a three-tube, most-probable-number procedure, the fluorescent-antibody method was able to detect Salmonella at a level of 0.036 organism per g. In addition to being a more rapid method for the detection of Salmonella, it has proven to be comparable to conventional culture procedures.
D001431:Bacteriological Techniques; D003470:Culture Media; D005396:Fish Products; D005455:Fluorescent Antibody Technique; D005516:Food Microbiology; D008460:Meat; D008722:Methods; D012475:Salmonella / Q000276:immunology / Q000302:isolation & purification*; D013997:Time Factors
D003470:Culture Media