Stabilization of colicin E2 by bovine serum albumin.
Colicin E2 was partially purified from Escherichia coli W3110. This preparation was remarkably stabilized by bovine serum albumin in a solution at neutral pH, as shown by dilution experiments and tests on heat stability of colicin. One killing unit of colicin E2 was estimated to correspond to one molecule of colicin E2, on the assumption of a molecular weight of 60,000.
D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D003087:Colicins / Q000032:analysis; D004355:Drug Stability; D004926:Escherichia coli / Q000032:analysis; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D012710:Serum Albumin, Bovine
D002247:Carbon Isotopes; D003087:Colicins; D012710:Serum Albumin, Bovine