Terminal cross-linking of DNA strands by an enzyme system from Escherichia coli infected with bacteriophage T4.
An enzyme system, purified 560-fold from Escherichia coli infected with bacteriophage T4, catalyzes the formation of a phosphodiester bond between the original 5'-phosphoryl end-group of a DNA strand and a 3'-hydroxyl group of the complementary strand. The product, a terminally cross-linked, spontaneously renaturable DNA duplex, has been characterized by chromatographic analysis, by sedimentation analysis, and by enzymatic digestion. Essential components of the enzyme system, which requires both ATP and Mg(++), include the T4-induced DNA ligase and a component found in extracts of uninfected E. coli, which is probably an exonuclease.
D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D002621:Chemistry; D002845:Chromatography; D003090:Coliphages; D004247:DNA / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D009691:Nucleic Acid Denaturation; D010759:Phosphorus Isotopes; D014316:Tritium
D010759:Phosphorus Isotopes; D014316:Tritium; D004247:DNA