Host-controlled restriction of T-even bacteriophages: relation of endonuclease I and T-even-induced nucleases to restriction.
Restriction of nonglucosylated T2 phage (T(*)2) as a function of bacterial growth state was the same for endonuclease I-containing and endonuclease I-deficient strains of Escherichia coli B. Furthermore, E. coli strains with various levels of restriction for T2 had comparable endonuclease I activities. It was also found that a T4 mutant temperature-sensitive for gene 46 and 47 functions was fully restricted at 42 C. It therefore appears that neither endonuclease I nor the phage-induced nucleases whose activities are blocked by mutations in genes 46 and 47 catalyze the initial event in restriction of nonglucosylated T-even phages.
D003090:Coliphages / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects; D003470:Culture Media; D004269:DNA, Bacterial / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development*; D009154:Mutation; D012260:Ribonucleases / Q000378:metabolism*; D014466:Ultraviolet Rays
D003470:Culture Media; D004269:DNA, Bacterial; D004279:DNA, Viral; D012260:Ribonucleases