Separation of Treponema pallidum from tissue debris through continuous-particle electrophoresis.
Treponema pallidum can only be cultured in living animal tissue, such as rabbit testes. However, the extract of these organisms from the testicular material leaves the T. pallidum contaminated with tissue debris. This paper describes the separation of T. pallidum from the debris by continuous-particle electrophoresis. The importance of equilibration time before electrophoresis is discussed.
D000818:Animals; D004586:Electrophoresis; D008297:Male; D008722:Methods; D011817:Rabbits; D013737:Testis / Q000382:microbiology; D014210:Treponema pallidum / Q000302:isolation & purification*