Synthesis of replicative form deoxyribonucleic acid and messenger ribonucleic acid by gene IV mutants of bacteriophage S13.
Gene IV mutants of bacteriophage S13 are known to be blocked in infectious replicative form (RF) DNA synthesis, producing only a small fraction of the RF formed by wild-type phage. This investigation shows that gene IV mutants form only parental RF and are blocked in the synthesis of any progeny RF, either infectious or noninfectious. This was determined by density labeling of RF in cells treated with mitomycin C to suppress host deoxyribonucleic acid (DNA) synthesis. RF synthesis was also studied in untreated cells, using methylated albumin columns to separate RF from host DNA. In this case it was also found that synthesis of progeny RF by gene IV mutants is negligible. It has been found by DNA-ribonucleic acid (RNA) hybridization experiments that gene IV mutants form at least as much or more messenger RNA than wild-type phage. Therefore, parental RF alone can form messenger RNA in appreciable amounts.
D001435:Bacteriophages / Q000378:metabolism*; D002247:Carbon Isotopes; D002845:Chromatography; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D004926:Escherichia coli / Q000187:drug effects; D006824:Hybridization, Genetic; D008937:Mitomycins / Q000494:pharmacology; D009154:Mutation; D012333:RNA, Messenger / Q000096:biosynthesis*; D012367:RNA, Viral / Q000096:biosynthesis*; D013936:Thymidine; D014316:Tritium; D014461:Ultracentrifugation; D014776:Virus Cultivation
D002247:Carbon Isotopes; D004279:DNA, Viral; D008937:Mitomycins; D012333:RNA, Messenger; D012367:RNA, Viral; D014316:Tritium; D013936:Thymidine