Technique for the isolation of ribonucleic acid-rich mutants from Escherichia coli.
A technique for the isolation of ribonucleic acid (RNA)-rich mutants of Escherichia coli is described. Mutagenized cells were centrifuged to isopycnic equilibrium on potassium tartrate or cesium sulfate gradients. Samples from the region of the gradients slightly denser than the majority of the cells were spread on agar plates, and the resulting clones were tested for increased RNA to protein ratios.
D002498:Centrifugation; D002586:Cesium / Q000494:pharmacology; D002999:Clone Cells; D003470:Culture Media; D004926:Escherichia coli / Q000187:drug effects*; D005827:Genetics, Microbial; D008722:Methods; D009154:Mutation; D011188:Potassium / Q000494:pharmacology; D012329:RNA, Bacterial / Q000302:isolation & purification*; D013431:Sulfates / Q000494:pharmacology; D013644:Tartrates / Q000494:pharmacology
D003470:Culture Media; D012329:RNA, Bacterial; D013431:Sulfates; D013644:Tartrates; D002586:Cesium; D011188:Potassium