title: Acetyl CoA carboxylase, II. Deomonstration of biotin-protein and biotin carboxylase subunits.
abstract: Previous work has shown that Escherichia coli acetyl CoA carboxylase is composed of two dissimilar protein components, E(a) which contains covalently bound biotin and forms E(a)-CO(2)-from HCO(3)- and ATP, and E(b) which is involved in the transfer of the carboxyl group from E(a)-CO(2)- to acetyl CoA, forming malonyl CoA. E(a) has been dissociated into two subunits at pH 9. One subunit, designated biotin carboxylase, catalyzes a model reaction, the ATP-dependent carboxylation of free (+)-biotin. The other subunit contains covalently bound which is carboxylated by the biotin carboxylase in the course of acetyl CoA carboxylation.
mesh_terms: D001426:Bacterial Proteins; D001710:Biotin; D002262:Carboxy-Lyases; D003065:Coenzyme A; D004926:Escherichia coli / Q000201:enzymology; D008970:Molecular Weight; D010047:Ovalbumin; D011485:Protein Binding
chemical_list: D001426:Bacterial Proteins; D001710:Biotin; D010047:Ovalbumin; D002262:Carboxy-Lyases; D003065:Coenzyme A