Isolation of catenated and replicating DNA molecules of colicin factor E1 from minicells.
Various catenated and replicating molecules of colicin E1 isolated from minicells have been identified in regions of neutral sucrose density gradients or cesium chloride-ethidium bromide density gradients. These colicin molecules have been found in those regions of the gradient where pulse-labeled DNA accumulates.
D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D002586:Cesium; D003087:Colicins / Q000302:isolation & purification*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000302:isolation & purification* / Q000378:metabolism; D004926:Escherichia coli / Q000032:analysis / Q000166:cytology / Q000378:metabolism*; D008854:Microscopy, Electron; D010617:Phenanthridines; D010759:Phosphorus Isotopes; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D003087:Colicins; D004269:DNA, Bacterial; D010617:Phenanthridines; D010759:Phosphorus Isotopes; D014316:Tritium; D002586:Cesium; D013936:Thymidine