Ultrastructure of deoxyribonucleic acid-membrane associations in Escherichia coli.
Areas of contact between deoxyribonucleic acid (DNA) and intracytoplasmic membrane are frequently seen in the "extra" membrane-forming strain Escherichia coli 0111a(1). By examination of serial sections, it has been estimated that these DNA-membrane associations occur in at least 60% of the extra membrane-containing cells. Most of the DNA masses contained only one contact area. Several cells in which the DNA had been stretched revealed individual fibers connecting to the membrane, suggesting a firm attachment of DNA to membrane. The areas of membrane associated with DNA fibers were usually between 100 and 500 nm in diameter, although some smaller areas were seen. Electron microscopic autoradiography of cells in which the replication forks were labeled showed grains over 24% of the profiles containing a contact area, whereas there were grains over only 16% of the profiles without a contact area. Data from autoradiographs of cells in which the label was "chased" away from the replication fork showed the reverse labeling pattern. These data indicate that the areas of contact between DNA and intracytoplasmic membranes seen in electron micrographs contain the DNA replication forks.
D001345:Autoradiography; D002462:Cell Membrane; D003593:Cytoplasm; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000166:cytology*; D008854:Microscopy, Electron; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D004269:DNA, Bacterial; D014316:Tritium; D013936:Thymidine