title: Isolation of catenated and replicating DNA molecules of colicin factor E1 from minicells.
abstract: Various catenated and replicating molecules of colicin E1 isolated from minicells have been identified in regions of neutral sucrose density gradients or cesium chloride-ethidium bromide density gradients. These colicin molecules have been found in those regions of the gradient where pulse-labeled DNA accumulates.
mesh_terms: D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D002586:Cesium; D003087:Colicins / Q000302:isolation & purification*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000302:isolation & purification* / Q000378:metabolism; D004926:Escherichia coli / Q000032:analysis / Q000166:cytology / Q000378:metabolism*; D008854:Microscopy, Electron; D010617:Phenanthridines; D010759:Phosphorus Isotopes; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
chemical_list: D003087:Colicins; D004269:DNA, Bacterial; D010617:Phenanthridines; D010759:Phosphorus Isotopes; D014316:Tritium; D002586:Cesium; D013936:Thymidine