Analysis for antigenic similarities among the 30S ribosomal proteins of Escherichia coli.
Antiserum was made against a single 30S protein, 30S-7. The amount of complement fixed by total 30S protein in the presence of this antiserum indicated that protein 30S-7 was the major antigen in the mixture of proteins. Each of the 30S ribosomal proteins was tested for cross-reactivity with anti-30S-7. This was done by determining if any of the other 30S proteins inhibited complement fixation by 30S-7. None of the other 30S proteins was found to inhibit complement fixation by 30S-7, indicating that 30S-7 is antigenically distinct from the other proteins.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D000942:Antigens, Bacterial / Q000032:analysis; D001426:Bacterial Proteins / Q000032:analysis* / Q000302:isolation & purification; D003168:Complement Fixation Tests; D003429:Cross Reactions; D004589:Electrophoresis, Disc; D004926:Escherichia coli / Q000032:analysis* / Q000276:immunology; D005827:Genetics, Microbial; D007106:Immune Sera; D011817:Rabbits; D012270:Ribosomes / Q000032:analysis*
D000941:Antigens; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007106:Immune Sera