The active centre of rabbit muscle triose phosphate isomerase. The site that is labelled by glycidol phosphate.
1. Glycidol (2,3-epoxypropanol) phosphate is a specific irreversible inhibitor of rabbit muscle triose phosphate isomerase (EC 5.3.1.1); the site of attachment has now been studied. 2. The labelled enzyme was digested with pepsin and a modified peptide isolated. The sequence of the peptide is: Ala-Tyr-Glu-Pro-Val-Trp. 3. It is the glutamic acid residue in this peptide that is labelled: the peptide is thus a gamma-glutamyl ester derived from glycerol phosphoric acid. The same site is labelled by a mixture of glycidol and inorganic phosphate. 4. Kinetic and stereochemical features of these reactions are discussed.
D000433:1-Propanol; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D004586:Electrophoresis; D004952:Esters; D004988:Ethers, Cyclic; D005971:Glutamates; D007535:Isomerases / Q000032:analysis*; D008956:Models, Chemical; D010434:Pepsin A; D010455:Peptides / Q000032:analysis; D010759:Phosphorus Isotopes; D011817:Rabbits
D004952:Esters; D004988:Ethers, Cyclic; D005971:Glutamates; D010455:Peptides; D010759:Phosphorus Isotopes; D000433:1-Propanol; D010434:Pepsin A; D007535:Isomerases