Membrane modifications in nutritionally induced filamentous Escherichia coli B.
Nutritionally induced filamentous cell forms of Escherichia coli B were examined for their morphological and biochemical lesions. The filamentous forms showed no significant alteration in total DNA concentration, RNA synthesis, ability to form beta-galactosidase in response to isopropylthiogalactoside, or insensitivity to actinomycin D as compared to the normal cell form. The filamentous cells showed a marked decrease in the ability to incorporate N-acetylglucosamine-UL-(14)C into a phenol-soluble glycoprotein fraction relative to the normal cell form or relative to strain E-26 of E. coli grown in the filament-inducing medium. The filaments yielded an envelope-specific phenol-soluble protein fraction markedly reduced in or lacking three proteins as determined by acrylamide gel electrophoresis. Amino acid analysis, and chemical and enzymatic treatments of the envelope-specific phenol-soluble proteins showed striking differences between the fractions obtained from normal and filamentous cells. Electron microscope studies of divalent cation-induced aggregates of the envelope proteins showed different aggregation patterns dependent upon the cell form yielding the protein fraction.
D000596:Amino Acids / Q000032:analysis; D050260:Carbohydrate Metabolism; D002247:Carbon Isotopes; D002462:Cell Membrane; D003470:Culture Media; D004269:DNA, Bacterial / Q000096:biosynthesis; D004589:Electrophoresis, Disc; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D005696:Galactosidases / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis*; D008854:Microscopy, Electron; D010743:Phospholipids / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D012329:RNA, Bacterial / Q000096:biosynthesis; D014529:Uridine / Q000378:metabolism
D000596:Amino Acids; D002247:Carbon Isotopes; D003470:Culture Media; D004269:DNA, Bacterial; D006023:Glycoproteins; D010743:Phospholipids; D011506:Proteins; D012329:RNA, Bacterial; D005696:Galactosidases; D005944:Glucosamine; D014529:Uridine