Studies on the high-sulphur proteins of reduced merino wool. Amino acid sequence of protein SCMKB-3B 3 .
The complete amino acid sequence of wool protein SCMKB-IIIB3 was determined. The peptides used for the sequence work were obtained by peptic and thermolysin digestions and were fractionated by chromatography on DEAE-cellulose, paper chromatography and electrophoresis. The peptides were analysed by dansyl-Edman degradation, mass spectrometry and tritium-labelling of C-terminal residues. The protein consists of 98 residues and has acetylalanine as N-terminal residue and carboxymethylcysteine as C-terminus. It is homologous with protein SCMKB-IIIB2 (Haylett & Swart, 1969). A salient feature of the sequence of protein SCMKB-IIIB3 is three consecutive cysteine residues.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D003545:Cysteine / Q000032:analysis; D003619:Dansyl Compounds; D004586:Electrophoresis; D013058:Mass Spectrometry; D010206:Papain; D010434:Pepsin A; D010455:Peptides / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D013820:Thermolysin; D014316:Tritium; D014935:Wool / Q000032:analysis*
D000596:Amino Acids; D003619:Dansyl Compounds; D010455:Peptides; D011506:Proteins; D014316:Tritium; D010206:Papain; D010434:Pepsin A; D013820:Thermolysin; D003545:Cysteine