DNA replication in Escherichia coli: location of recently incorporated thymidine within molecules of high molecular weight DNA.
More than 50% of the [(3)H]thymidine incorporated during short pulses into the DNA of Escherichia coli 15T(-) can be extracted by alkali as high molecular weight DNA. Density gradient centrifugation and digestion with exonuclease I suggest that these large pieces of DNA are composed of newly synthesized DNA attached to pre-existing material at the 3' end of the molecule.
D001976:Bromouracil / Q000378:metabolism; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000302:isolation & purification; D004261:DNA Replication; D003851:Deoxyribonucleases / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D006868:Hydrolysis; D008970:Molecular Weight; D013395:Sucrose; D013936:Thymidine / Q000378:metabolism*; D013941:Thymine / Q000378:metabolism; D014316:Tritium
D002247:Carbon Isotopes; D014316:Tritium; D001976:Bromouracil; D013395:Sucrose; D004247:DNA; D003851:Deoxyribonucleases; D013941:Thymine; D013936:Thymidine