Effect of sodium fluorescein and plating medium on recovery of irradiated Escherichia coli and Serratia marcescens from aerosols.
Irradiation of aerosols of either Escherichia coli or Serratia marcescens with simulated solar (xenon) radiation caused a significant decrease in viability. When sodium fluorescein was employed to determine the physical loss of organisms from the aerosol, an additional adverse effect upon survival was noted. The decay curves indicated that at least two mechanisms of inactivation were operative, one due to aerosolization, the other to irradiation. After collection from aerosols, both species of microorganisms grew better on blood agar base than on Casitone agar, but this finding did not appear to be related to the effect of irradiation.
D000362:Agar; D000391:Air Microbiology; D001431:Bacteriological Techniques; D001769:Blood; D003470:Culture Media; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification* / Q000528:radiation effects; D005452:Fluoresceins / Q000494:pharmacology*; D011830:Radiation Effects; D012706:Serratia marcescens / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification* / Q000528:radiation effects; D012964:Sodium; D013223:Statistics as Topic; D013472:Sunlight; D014978:Xenon
D003470:Culture Media; D005452:Fluoresceins; D014978:Xenon; D000362:Agar; D012964:Sodium