Mutants of Escherichia coli sensitive to antibiotics.
Mutants of Escherichia coli sensitive to the antibiotic synergistin A, an inhibitor of protein synthesis, were isolated. These mutants were pleiotropic, being also sensitive to a large number of unrelated antibiotics and to lysis by detergents. These pleiotropic responses indicated that the mutations affected cell wall or membrane synthesis. Consequently, selection for antibiotic-sensitive mutants constitutes a useful means for isolating cell wall or membrane mutants.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002247:Carbon Isotopes; D002462:Cell Membrane; D002473:Cell Wall; D003470:Culture Media; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D005827:Genetics, Microbial; D007930:Leucine / Q000378:metabolism; D008242:Lysogeny; D008826:Microbial Sensitivity Tests; D009154:Mutation; D012329:RNA, Bacterial / Q000096:biosynthesis; D013501:Surface-Active Agents / Q000494:pharmacology; D014498:Uracil / Q000378:metabolism
D000900:Anti-Bacterial Agents; D002247:Carbon Isotopes; D003470:Culture Media; D012329:RNA, Bacterial; D013501:Surface-Active Agents; D014498:Uracil; D007930:Leucine