Antibody-mediated suppression of the immune response in vitro. II. A new approach to the phenomenon of immunological tolerance.
Immunological tolerance to H antigens of Salmonella adelaide may be induced in vitro by the exposure of mouse spleen cells for 6 hr to an immunogenic dose of polymerized flagellin in the presence of low concentrations of specific antibody. Such antibody-mediated tolerance requires an optimal antigen: antibody ratio for its induction. A shift in this ratio in favor of the antibody concentration results in failure of tolerance induction and leads to immune suppression commonly known as antibody-mediated feedback inhibition which is not analogous to immunological tolerance. Fragment A of flagellin fails to induce immunological tolerance in vitro. Tolerance to polymerized flagellin may however be induced in vitro, provided the spleen cells are exposed to fragment A in the presence of specific antibody for 6 hr. The results are discussed in the light of current theories of the mechanism of tolerance induction.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000917:Antibody Formation; D000941:Antigens; D046508:Culture Techniques; D005260:Female; D006462:Hemolytic Plaque Technique; D007108:Immune Tolerance; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D051379:Mice; D008954:Models, Biological; D011108:Polymers; D012475:Salmonella / Q000276:immunology; D013154:Spleen / Q000166:cytology; D013997:Time Factors
D000906:Antibodies; D000941:Antigens; D011108:Polymers