Inactivation of ribosomes in vitro by colicin E 3 .
Cell-free protein synthesizing extracts incubated with purified preparations of colicin E(3) showed a marked decrease of their protein synthesizing activity. Incubation of ribosomes prepared from S-30 extracts with E(3) resulted in their inactivation. Their 16S RNA was found to lose a terminal fragment, similar in size to the fragment released upon in vivo treatment with E(3). Extracts derived from colicinogenic strains are resistant to the action of E(3)in vitro.
D001345:Autoradiography; D001426:Bacterial Proteins / Q000096:biosynthesis*; D002247:Carbon Isotopes; D002474:Cell-Free System; D003087:Colicins / Q000494:pharmacology*; D004926:Escherichia coli / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D009154:Mutation; D010649:Phenylalanine / Q000378:metabolism; D011119:Polynucleotides / Q000378:metabolism; D012335:RNA, Ribosomal / Q000032:analysis / Q000096:biosynthesis*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D013696:Temperature; D014316:Tritium; D014498:Uracil / Q000378:metabolism
D001426:Bacterial Proteins; D002247:Carbon Isotopes; D003087:Colicins; D011119:Polynucleotides; D012335:RNA, Ribosomal; D014316:Tritium; D010649:Phenylalanine; D014498:Uracil; D008239:Lysine