title: Precursor ribosomal ribonucleic acid and ribosome accumulation in vivo during the recovery of Salmonella typhimurium from thermal injury.
abstract: When cells of S. typhimurium were heated at 48 C for 30 min in phosphate buffer (pH 6.0), they became sensitive to Levine Eosin Methylene Blue Agar containing 2% NaCl (EMB-NaCl). The inoculation of injured cells into fresh growth medium supported the return of their normal tolerance to EMB-NaCl within 6 hr. The fractionation of ribosomal ribonucleic acid (rRNA) from unheated and heat-injured cells by polyacrylamide gel electrophoresis demonstrated that after injury the 16S RNA species was totally degraded and the 23S RNA was partially degraded. Sucrose gradient analysis demonstrated that after injury the 30S ribosomal subunit was totally destroyed and the sedimentation coefficient of the 50S particle was decreased to 47S. During the recovery of cells from thermal injury, four species of rRNA accumulated which were demonstrated to have the following sedimentation coefficients: 16, 17, 23, and 24S. Under identical recovery conditions, 22, 26, and 28S precursors of the 30S ribosomal subunit and 31 and 48S precursors of the 50S ribosomal subunit accumulated along with both the 30 and 50S mature particles. The addition of chloramphenicol to the recovery medium inhibited both the maturation of 17S RNA and the production of mature 30S ribosomal subunits, but permitted the accumulation of a single 22S precursor particle. Chloramphenicol did not affect either the maturation of 24S RNA or the mechanism of formation of 50S ribosomal subunits during recovery. Very little old ribosomal protein was associated with the new rRNA synthesized during recovery. New ribosomal proteins were synthesized during recovery and they were found associated with the new rRNA in ribosomal particles. The rate-limiting step in the recovery of S. typhimurium from thermal injury was in the maturation of the newly synthesized rRNA.
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D002701:Chloramphenicol / Q000494:pharmacology; D003470:Culture Media; D004352:Drug Resistance, Microbial; D004589:Electrophoresis, Disc; D005827:Genetics, Microbial; D006358:Hot Temperature; D007930:Leucine / Q000378:metabolism; D008751:Methylene Blue / Q000494:pharmacology; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D010759:Phosphorus Isotopes; D012329:RNA, Bacterial / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism; D012270:Ribosomes / Q000032:analysis / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000032:analysis / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D013053:Spectrophotometry; D013395:Sucrose; D013997:Time Factors; D014316:Tritium; D014498:Uracil / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002247:Carbon Isotopes; D003470:Culture Media; D010759:Phosphorus Isotopes; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D014316:Tritium; D012965:Sodium Chloride; D014498:Uracil; D013395:Sucrose; D002701:Chloramphenicol; D007930:Leucine; D008751:Methylene Blue