title: Mutants of Escherichia coli sensitive to antibiotics.
abstract: Mutants of Escherichia coli sensitive to the antibiotic synergistin A, an inhibitor of protein synthesis, were isolated. These mutants were pleiotropic, being also sensitive to a large number of unrelated antibiotics and to lysis by detergents. These pleiotropic responses indicated that the mutations affected cell wall or membrane synthesis. Consequently, selection for antibiotic-sensitive mutants constitutes a useful means for isolating cell wall or membrane mutants.
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002247:Carbon Isotopes; D002462:Cell Membrane; D002473:Cell Wall; D003470:Culture Media; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D005827:Genetics, Microbial; D007930:Leucine / Q000378:metabolism; D008242:Lysogeny; D008826:Microbial Sensitivity Tests; D009154:Mutation; D012329:RNA, Bacterial / Q000096:biosynthesis; D013501:Surface-Active Agents / Q000494:pharmacology; D014498:Uracil / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D002247:Carbon Isotopes; D003470:Culture Media; D012329:RNA, Bacterial; D013501:Surface-Active Agents; D014498:Uracil; D007930:Leucine