New method for extraction of ribonucleic acid and polyribosomes from Schizosaccharomyces pombe.
A new method for extracting ribosomal ribonucleic acid (RNA) and polyribosomes from Schizosaccharomyces pombe, a fission yeast, has been developed. Cells grown in the presence of 2-deoxyglucose were found to be more sensitive to the action of Glusulase; a short period of treatment (10 to 20 min) at 0 C was sufficient to convert the cells to osmotically sensitive structures. From these, ribosomal RNA and polyribosomes were readily prepared.
D001203:Ascomycota / Q000032:analysis* / Q000254:growth & development; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D003080:Cold Temperature; D003470:Culture Media; D003902:Detergents; D005966:Glucuronidase; D006601:Hexoses; D008722:Methods; D008858:Microscopy, Phase-Contrast; D012335:RNA, Ribosomal / Q000302:isolation & purification*; D012270:Ribosomes / Q000032:analysis*; D012964:Sodium; D013395:Sucrose; D013429:Sulfatases; D013431:Sulfates
D003470:Culture Media; D003902:Detergents; D006601:Hexoses; D012335:RNA, Ribosomal; D013431:Sulfates; D013395:Sucrose; D012964:Sodium; D013429:Sulfatases; D005966:Glucuronidase