Rapid fluorescent-antibody stain technique with group A streptococci.
A rapid fluorescent-antibody stain technique used with young broth cultures of trypsinized group A streptococci is described. Results from 1,214 trypsinized cultures of group A streptococci employing the rapid stain method were equivalent, both in specificity and sensitivity, when compared to results obtained from identical non-trypsinized cultures stained with the longer standard technique of Cherry, Goldman, and Carski. Moreover, the rapid method requires less than 2 min to complete.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D008856:Microscopy, Fluorescence; D011817:Rabbits; D012703:Serotyping; D013291:Streptococcus / Q000276:immunology*; D014357:Trypsin
D000941:Antigens; D007106:Immune Sera; D014357:Trypsin