Purification and DNA synthesis in cell-free extracts: properties of DNA polymerase II.
The major DNA-synthesizing enzyme present in Pol A(1) (-)Escherichia coli (DNA polymerase II) has been purified to homogeneity as judged by polyacrylamide gel electrophoresis. The enzyme requires all four deoxynucleoside triphosphates, Mg(++), NH(4) (+), and native DNA for maximal activity. The enzyme activity is sensitive to sulfhydryl reagents and is insensitive to anti-DNA polymerase I antiserum. A second DNA-synthesizing enzyme, present in low amounts, has been identified in Pol A(1) (-) extracts. The relationship of this enzyme to DNA polymerases I and II is discussed.
D002474:Cell-Free System; D055598:Chemical Phenomena; D002621:Chemistry; D002845:Chromatography; D002848:Chromatography, DEAE-Cellulose; D004254:DNA Nucleotidyltransferases / Q000302:isolation & purification*; D004261:DNA Replication; D004586:Electrophoresis; D004926:Escherichia coli / Q000201:enzymology; D006651:Histocytochemistry; D066298:In Vitro Techniques; D009154:Mutation; D013698:Templates, Genetic
D004254:DNA Nucleotidyltransferases