Monitoring of low-level virus in natural waters.
The insoluble polyelectrolyte technique for concentrating virus is extended to extremely low virus levels. The effectiveness of this method employing a coliphage T2 model is a constant 20% over a range of virus levels from 10(3) to 10(-4) plaque-forming units/ml. The efficiency of the method is dependent upon pH control during the concentration phase. Although the study was initiated to develop a method for quantitating the effectiveness of water and wastewater treatment methods for the removal of viruses from waters at low concentrations, the potential of the technique for efficient monitoring of natural waters is apparent.
D002499:Centrifugation, Density Gradient; D003090:Coliphages / Q000302:isolation & purification*; D004573:Electrolytes; D004926:Escherichia coli; D006863:Hydrogen-Ion Concentration; D008722:Methods; D008954:Models, Biological; D012722:Sewage; D013936:Thymidine; D014316:Tritium; D014871:Water Microbiology; D014876:Water Pollution; D014881:Water Supply
D004573:Electrolytes; D012722:Sewage; D014316:Tritium; D013936:Thymidine