Role of gene 46 in bacteriophage T4 deoxyribonucleic acid synthesis.
In an attempt to establish whether Escherichia coli B infected with N130 (an amber mutant defective in gene 46) is recombination-deficient, the postinfection fate of (14)C-labeled N130 parental deoxyribonucleic acid (DNA) was followed, its amount in complex with the host cell membrane being determined in sucrose gradients after mild lysis of the infected cells. The parental DNA was found to undergo gradual detachment from the membrane during infection. Pulse-chase experiments similarly showed that newly synthesized DNA is normally attached to the host cell membrane and is detached by endonucleolytic breakage at a late stage of infection. The conclusion is that only attached DNA molecules are replicated by membrane-bound replicase, whereas those detached by endonucleolytic breakage are not. It thus seems that the gene 46 product controls the activity of a nuclease whose main function is recombination of DNA nicked by endonuclease, thereby attaching it to the host cell membrane. The rate of T4 DNA synthesis is apparently governed by the efficiency of recombination. Supporting evidence was found in experiments with the double mutant N130 x N134 (genes 46, 33).
D002247:Carbon Isotopes; D002462:Cell Membrane / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002701:Chloramphenicol / Q000494:pharmacology; D002712:Chlorides; D003090:Coliphages / Q000378:metabolism*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis; D004279:DNA, Viral / Q000096:biosynthesis*; D003851:Deoxyribonucleases / Q000096:biosynthesis* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism; D005796:Genes; D005827:Genetics, Microbial; D005990:Glycerol / Q000378:metabolism; D009154:Mutation; D011995:Recombination, Genetic; D013395:Sucrose; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D002247:Carbon Isotopes; D002712:Chlorides; D004269:DNA, Bacterial; D004279:DNA, Viral; D014316:Tritium; D002586:Cesium; D013395:Sucrose; D002701:Chloramphenicol; D003851:Deoxyribonucleases; D005990:Glycerol; D013936:Thymidine