DNA polymerase required for rapid repair of x-ray--induced DNA strand breaks in vivo.
A much higher yield of DNA single-strand breaks was obtained in the DNA polymerase-deficient mutant Escherichia coli K-12 pol A1 after a given dose of x-rays than had been found before in Escherichia coli. The increased yield of single-strand breaks was due to the absence of a rapid repair system, which had not been described in Escherichia coli K-12. This absence probably accounts for the x-ray sensitivity of the pol A1 mutant. The rapid repair system can be reversibly inhibited in pol+ cells.
D002499:Centrifugation, Density Gradient; D002876:Chromosomes, Bacterial; D004254:DNA Nucleotidyltransferases; D004269:DNA, Bacterial / Q000528:radiation effects*; D004926:Escherichia coli; D005827:Genetics, Microbial; D009154:Mutation; D011830:Radiation Effects
D004269:DNA, Bacterial; D004254:DNA Nucleotidyltransferases