A method for measuring the content of a specific messenger ribonucleic acid in Escherichia coli.
A method is described for measuring the porportion of a specific messenger RNA in the total RNA extracted from pulse-labelled cells. A model system consisting of total ribosomal RNA and Escherichia coli DNA is used to validate the method and to define the conditions under which it can be used.
D002247:Carbon Isotopes; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000032:analysis*; D008722:Methods; D009693:Nucleic Acid Hybridization; D010759:Phosphorus Isotopes; D012329:RNA, Bacterial / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis*; D012335:RNA, Ribosomal
D002247:Carbon Isotopes; D004269:DNA, Bacterial; D010759:Phosphorus Isotopes; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012335:RNA, Ribosomal