Chain initiation during polypeptide synthesis in cell-free bacterial systems programmed with a plant viral messenger. The formation of N-acetylphenylalanylisoleucyl-tRNA on the messenger-ribosome complex.
D000215:Acylation; D001665:Binding Sites; D002247:Carbon Isotopes; D002474:Cell-Free System; D002854:Chromatography, Paper; D004590:Electrophoresis, Paper; D004926:Escherichia coli; D005815:Genetic Code; D005827:Genetics, Microbial; D006160:Guanosine Triphosphate; D007532:Isoleucine / Q000378:metabolism; D008274:Magnesium; D000455:Medicago sativa; D010452:Peptide Biosynthesis; D010441:Peptide Chain Elongation, Translational; D010442:Peptide Chain Initiation, Translational; D010455:Peptides / Q000032:analysis; D010649:Phenylalanine / Q000378:metabolism; D010942:Plant Viruses; D011691:Puromycin / Q000494:pharmacology; D012333:RNA, Messenger; D012343:RNA, Transfer / Q000378:metabolism*; D012367:RNA, Viral; D012270:Ribosomes / Q000378:metabolism*; D014316:Tritium
D002247:Carbon Isotopes; D010455:Peptides; D012333:RNA, Messenger; D012367:RNA, Viral; D007532:Isoleucine; D014316:Tritium; D010649:Phenylalanine; D011691:Puromycin; D006160:Guanosine Triphosphate; D012343:RNA, Transfer; D008274:Magnesium