title: Practical method for isolation of anerobic bacteria in the clinical laboratory.
abstract: An inexpensive but practical and simple method is described for providing an environment suitable for the maintenance and survival of clinically significant anaerobic bacteria, consistent with the needs of a large-volume laboratory. This modification of the GasPak procedure is based on two concepts. One involved the storage of "freshly prepared" plating medium under a constant stream of carbon dioxide. The other concept was to use an anaerobe jar, continuously flushed with oxygen-free carbon dioxide, as a "holding receptacle" for the inoculated media Experience with this modification has shown that the number of isolations of anaerobic bacteria from clinical material can be increased significantly.
mesh_terms: D000693:Anaerobiosis; D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis*; D001431:Bacteriological Techniques / Q000295:instrumentation; D002245:Carbon Dioxide; D003470:Culture Media; D003937:Diagnosis, Differential; D006801:Humans; D012621:Seasons
chemical_list: D003470:Culture Media; D002245:Carbon Dioxide