title: Replication and repair of DNA in cells of Escherichia coli treated with toluene.
abstract: DNA synthesis has been studied in Escherichia coli cells made permeable to nucleotides by treatment with toluene. Replicative synthesis, as distinguished from repair synthesis, occurs at a rate comparable to that observed in vivo; it is dependent on the presence of all four deoxyribonucleoside triphosphates, but does not require exogenous DNA; and it is stimulated by ATP. Furthermore, replicative synthesis can be abolished at the restrictive temperature in DNA temperature-sensitive mutants. N-ethylmaleimide completely inhibits this type of synthesis, whereas it does not inhibit repair synthesis. Repair synthesis further differs from replicative synthesis in the following points: it does not require ATP; it persists at the restrictive temperature in DNA temperature-sensitive mutants; it can be induced by endogenous or exogenous nuclease activity; and its demonstration requires a Pol(+) strain.
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D004260:DNA Repair; D004261:DNA Replication / Q000187:drug effects; D004269:DNA, Bacterial / Q000032:analysis / Q000096:biosynthesis*; D003851:Deoxyribonucleases / Q000494:pharmacology; D003864:Depression, Chemical; D004357:Drug Synergism; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D005033:Ethylmaleimide / Q000494:pharmacology; D009154:Mutation; D010759:Phosphorus Isotopes; D013268:Stimulation, Chemical; D013696:Temperature; D013997:Time Factors; D014050:Toluene / Q000494:pharmacology*; D014316:Tritium
chemical_list: D004269:DNA, Bacterial; D010759:Phosphorus Isotopes; D014316:Tritium; D014050:Toluene; D000255:Adenosine Triphosphate; D003851:Deoxyribonucleases; D005033:Ethylmaleimide