Identification of the forms of vitamin B 6  present in the culture media of "vitamin B 6  control" mutants.
An Escherichia coli mutant resistant to isoniazid (WG497) contained 0.6 mumole of extracellular pyridoxamine and pyridoxamine phosphate in the early stationary phase. A suppressed lysine mutant (AT1024) contained 1.4 mumoles of pyridoxal phosphate under the same conditions. The internal concentration of vitamin B(6) was one-half of normal for AT1024 and increased fivefold for WG497.
D001681:Biological Assay; D002463:Cell Membrane Permeability; D002474:Cell-Free System; D003470:Culture Media / Q000032:analysis*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000032:analysis* / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D005827:Genetics, Microbial; D007538:Isoniazid / Q000494:pharmacology; D009154:Mutation; D010847:Picolines / Q000378:metabolism*; D011732:Pyridoxal Phosphate / Q000032:analysis*; D011736:Pyridoxine / Q000032:analysis* / Q000096:biosynthesis; D014050:Toluene; D015003:Yeasts
D003470:Culture Media; D010847:Picolines; D014050:Toluene; D011732:Pyridoxal Phosphate; D011736:Pyridoxine; D007538:Isoniazid