Purification and properties of 3-deoxy-d-arabinoheptulosonic acid-7-phosphate synthetase (phe) from a lambda aroG+ transductant of Escherichia coli.
By incorporating aroG, the structural gene for 3-deoxy-d-arabinoheptulosonic acid-7-phosphate (DAHP) synthetase (phe), into the genome of a heat-inducible susR60 mutant of phage lambda, it has been possible to increase the intracellular levels of DAHP synthetase (phe) in a lysogenized strain of Escherichia coli some 15-fold over levels found in the wild-type strain. By using this strain, the enzyme has been purified approximately 2,000-fold compared with wild type, and various kinetic parameters of the purified enzyme have been studied. In contrast to previous reports, the inhibition by phenylalanine was found to exhibit sigmoidal kinetics, suggestive of cooperative interactions between phenylalanine binding sites. Stimulation of enzyme activity by Co(2+) was minimal (14%).
D000446:Aldehyde-Lyases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism; D000645:Ammonium Sulfate; D002474:Cell-Free System; D011232:Chemical Precipitation; D002845:Chromatography; D002850:Chromatography, Gel; D003035:Cobalt / Q000494:pharmacology; D003090:Coliphages; D003470:Culture Media; D004589:Electrophoresis, Disc; D004790:Enzyme Induction; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development; D005796:Genes; D006539:Heptoses; D006358:Hot Temperature; D006882:Hydroxyapatites; D008242:Lysogeny; D008970:Molecular Weight; D009154:Mutation; D010649:Phenylalanine / Q000494:pharmacology; D010728:Phosphoenolpyruvate / Q000378:metabolism; D013780:Tetroses / Q000378:metabolism; D014161:Transduction, Genetic; D014465:Ultrasonics; D014466:Ultraviolet Rays; D014732:Vibration
D003470:Culture Media; D006539:Heptoses; D006882:Hydroxyapatites; D013780:Tetroses; D003035:Cobalt; D010649:Phenylalanine; D010728:Phosphoenolpyruvate; D000446:Aldehyde-Lyases; D000645:Ammonium Sulfate