Studies of membrane formation in Tetrahymena pyriformis. II. Isolation and lipid analysis of cell fractions.
A method has been devised to fractionate cells of Tetrahymena pyriformis, yielding pure or highly enriched preparations of cilia, cilia-associated soluble material, pellicles, mitochondria, microsomes, and postmicrosomal supernatant. The method prevents the destructive action of lipolytic enzymes commonly associated with this organism. Analysis of the membrane lipids of these fractions reveals significant differences in lipid composition. Most noteworthy are the high concentrations of phosphonolipid and tetrahymanol in the surface membranes.
D000818:Animals; D002462:Cell Membrane / Q000032:analysis*; D002855:Chromatography, Thin Layer; D002923:Cilia / Q000032:analysis; D003470:Culture Media; D006651:Histocytochemistry; D006652:Histological Techniques; D008055:Lipids / Q000032:analysis*; D008722:Methods; D008854:Microscopy, Electron; D008861:Microsomes / Q000032:analysis; D008928:Mitochondria / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D013768:Tetrahymena / Q000166:cytology*
D003470:Culture Media; D008055:Lipids; D010743:Phospholipids