Purification of the araC protein.
The araC gene product, a regulatory protein required for expression of the L-arabinose operon, has been purified by affinity chromatography on Sepharose 4B to which 4-aminophenyl-beta-D-6-deoxygalactopyranoside (an anti-inducer of the L-arabinose operon) had been covalently attached by means of a 4-aminophenylbutanamido side chain. Evidence is presented showing that the protein binds specifically to ara DNA.
D000814:Aniline Compounds / Q000494:pharmacology; D001089:Arabinose; D001345:Autoradiography; D001426:Bacterial Proteins / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D004269:DNA, Bacterial; D004589:Electrophoresis, Disc; D004926:Escherichia coli / Q000032:analysis; D005690:Galactose / Q000494:pharmacology; D005809:Genes, Regulator; D006027:Glycosides / Q000494:pharmacology; D009876:Operon; D010759:Phosphorus Isotopes; D011485:Protein Binding
D000814:Aniline Compounds; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D006027:Glycosides; D010759:Phosphorus Isotopes; D001089:Arabinose; D005690:Galactose