Reagent strips and conventional tests for acid production from mannitol and coagulase activity of Staphylococcus: a comparative study.
A total of 244 Staphylococcus strains were tested simultaneously for acid production from mannitol and for coagulase activity with reagent-impregnated paper strips and with their conventional counterparts. Significant correlation was obtained with 97.9% of the strains for mannitol and with 95% for the coagulase test. The paper strip method is a combined test for both mannitol and coagulase tests, thus making it more convenient and simpler than conventional methods. The results are obtained rapidly within 6 hr by the paper strip method. However, as the paper strip method is designed for the aerobic system, the conventional tests were also carried out under aerobic conditions to compare the results.
D000143:Acids / Q000096:biosynthesis*; D001431:Bacteriological Techniques; D003030:Coagulase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008353:Mannitol / Q000378:metabolism*; D010100:Oxygen; D013210:Staphylococcus / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism*
D000143:Acids; D003030:Coagulase; D007202:Indicators and Reagents; D008353:Mannitol; D010100:Oxygen