Preparation and testing of polyvalent conjugates for fluorescent-antibody detection of salmonellae.
A polyvalent OH conjugate for Salmonella O groups A through I, K, L, and O was prepared and tested against pure cultures of salmonellae, nonsalmonellae, and a variety of food, fecal, and environmental specimens. Examination of pure cultures revealed that the conjugate gave negligible staining with representative strains of Shigella, Proteus, Providence, Serratia, and Pseudomonas. However, it stained 12% of the Escherichia coli and Citrobacter freundii strains and 36% of the Arizona strains. Over 1,200 specimens of various types were examined by both fluorescent-antibody (FA) and cultural procedures. Results indicate that, when used with discretion, FA screening can be a useful tool for rapid presumptive indication of the presence of salmonellae. The need for careful selection of strains used for preparing antisera and the importance of adequate evaluation of Salmonella FA reagents are discussed.
D000645:Ammonium Sulfate; D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D001431:Bacteriological Techniques; D003470:Culture Media; D004755:Enterobacteriaceae / Q000276:immunology; D004925:Escherichia / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D005516:Food Microbiology; D005990:Glycerol; D006801:Humans; D007106:Immune Sera; D007120:Immunochemistry; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D008722:Methods; D008854:Microscopy, Electron; D011511:Proteus / Q000276:immunology; D011549:Pseudomonas / Q000276:immunology; D011817:Rabbits; D012475:Salmonella / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification*; D012703:Serotyping; D012705:Serratia / Q000276:immunology; D012760:Shigella / Q000276:immunology; D013045:Species Specificity
D000942:Antigens, Bacterial; D003470:Culture Media; D005452:Fluoresceins; D007106:Immune Sera; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D005990:Glycerol; D000645:Ammonium Sulfate