Studies on resistance transfer factor deoxyribonucleic acid in Escherichia coli.
A variant of the derepressed R factor, R1, which does not contain any of the drug resistance markers, and represents, in large part, the resistance transfer factor (RTF) was studied in Escherichia coli. RTF deoxyribonucleic acid (DNA) was specifically labeled in a female cell after conjugation. Physical characterization of the molecule showed that RTF possessed an average molecular weight of 50 x 10(6) daltons and a buoyant density of 1.709 g/cm(3). By comparison to R1, we calculate that the region of DNA carrying the drug resistance genes is therefore about 20% of the R1 molecule and has a buoyant density of approximately 1.716 g/cm(3). These results support the hypothesis that the single species of R-factor DNA observed in E. coli represents a composite of the 1.709 and 1.716 g/cm(3) replicons seen in Proteus.
D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D004269:DNA, Bacterial / Q000378:metabolism*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000378:metabolism* / Q000528:radiation effects; D005827:Genetics, Microbial; D006824:Hybridization, Genetic; D008970:Molecular Weight; D011830:Radiation Effects; D013941:Thymine / Q000378:metabolism; D014316:Tritium
D002247:Carbon Isotopes; D004269:DNA, Bacterial; D014316:Tritium; D013941:Thymine