Sensitivity of intracellular bacteriophage lambda to colicin CA42-E2.
Treatment of Escherichia coli K-12 infected by lambda CIts857 with colicin CA42-E2 resulted in partial inhibition of the infectious process. Uninfected bacteria were killed by colicin with a probability of about five times that with which similarly treated lambda-infected bacteria lose plaque-forming ability. The lambda deoxyribonucleic acid (DNA), when present in a bacterial cell either as the replicating DNA of infectious phage or as the nonreplicating DNA of superinfecting phage, was degraded to acid-soluble material after colicin treatment. Analysis of the intermediates of DNA breakdown has revealed that degradation of the DNA to acid-soluble material is preceded by endonucleolytic fragmentation of the chromosome at a limited number of sites. This is the same mechanism of degradation previously observed for E. coli DNA after colicin treatment.
D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D003087:Colicins / Q000494:pharmacology*; D003090:Coliphages / Q000187:drug effects* / Q000254:growth & development; D004269:DNA, Bacterial / Q000302:isolation & purification; D004279:DNA, Viral / Q000302:isolation & purification; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D008242:Lysogeny; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D010710:Phosphates; D013395:Sucrose; D013936:Thymidine; D013941:Thymine; D014316:Tritium; D014779:Virus Replication / Q000187:drug effects*
D002247:Carbon Isotopes; D003087:Colicins; D004269:DNA, Bacterial; D004279:DNA, Viral; D010710:Phosphates; D014316:Tritium; D013395:Sucrose; D013941:Thymine; D013936:Thymidine