Control of cell growth in bacteria: experiments with thymine starvation.
When cells of Escherichia coli THU were starved for thymine, they continued to grow without division for at least two successive volume doublings at their initial rate. Within experimental error this average rate of volume increase, 0.21 mum(3) per hr, was identical with that observed in control cultures during two generations of growth in the presence of thymine. This growth rate was also independent of the age of the cells at the time of starvation. These results are consistent with the hypothesis, proposed earlier, that growth rates are controlled by uptake sites for binding, transport, or accumulation of compounds into the cell, that the number of these sites is constant throughout most of the cell cycle, and that this number doubles near or at cell division.
D000085:Acetates; D001431:Bacteriological Techniques; D002455:Cell Division; D002499:Centrifugation, Density Gradient; D003470:Culture Media
D000085:Acetates; D003470:Culture Media