Bovine pepsinogens and pepsins. The sequence around a reactive aspartyl residue.
The specific inhibitor, N-diazoacetylnorleucine methyl ester reacts stoicheiometrically with bovine pepsin resulting in a simultaneous loss of all enzymic activity. A peptide containing a modified aspartyl group was isolated from bovine pepsin labelled with (14)C-labelled inhibitor. The aspartic acid residue is presumed to be part of the active centre and is in the same heptapeptide sequence as in porcine pepsin: Ile-Val-Asp-Thr-Gly-Thr-Ser.
D000085:Acetates; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000614:Aminocaproates; D000818:Animals; D001224:Aspartic Acid / Q000032:analysis*; D001391:Azo Compounds; D001665:Binding Sites; D002247:Carbon Isotopes; D002268:Carboxypeptidases; D002417:Cattle; D009646:Norleucine / Q000031:analogs & derivatives; D010434:Pepsin A / Q000032:analysis* / Q000037:antagonists & inhibitors; D010455:Peptides / Q000032:analysis; D013552:Swine
D000085:Acetates; D000596:Amino Acids; D000614:Aminocaproates; D001391:Azo Compounds; D002247:Carbon Isotopes; D010455:Peptides; D001224:Aspartic Acid; C000909:N-diazoacetylnorleucine methyl ester; D009646:Norleucine; D002268:Carboxypeptidases; D010434:Pepsin A