Temperature sensitivity of maltose utilization and lambda resistance in Escherichia coli B.
Escherichia coli B strains that have acquired the malB region from E. coli K-12 are able to utilize maltose and to adsorb phage lambda when grown at 30 C, but when grown at 40 C they do not absorb phage lambda and are devoid of amylomaltase activity. These Mal(ts) Lam(ts) cells can be mutated or transduced to become able to grow on maltose at 40 C, but they still have no detectable amylomaltase activity nor functional lambda receptors at that temperature. This Mal(40) phenotype is governed by a gene located near or at malA. It is suggested that the temperature sensitivity of both characters results from a defect in malT. However, transduction of malA from E. coli B to E. coli K-12 results in a wild-type phenotype, whereas E. coli B cells that have acquired malA from E. coli K-12 donors are still temperature sensitive for both amylomaltase and lambda-receptor production.
D003090:Coliphages; D003470:Culture Media; D004926:Escherichia coli / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D005796:Genes; D005827:Genetics, Microbial; D005964:Glucosyltransferases / Q000378:metabolism; D008242:Lysogeny; D008320:Maltose / Q000378:metabolism*; D009154:Mutation; D010641:Phenotype; D013053:Spectrophotometry; D013696:Temperature; D014161:Transduction, Genetic
D003470:Culture Media; D008320:Maltose; D005964:Glucosyltransferases