Metabolism of exogenous purine bases and nucleosides by Salmonella typhimurium.
Purine-requiring mutants of Salmonella typhimurium LT2 containing additional mutations in either adenosine deaminase or purine nucleoside phosphorylase have been constructed. From studies of the ability of these mutants to utilize different purine compounds as the sole source of purines, the following conclusions may be drawn. (i) S. typhimurium does not contain physiologically significant amounts of adenine deaminase and adenosine kinase activities. (ii) The presence of inosine and guanosine kinase activities in vivo was established, although the former activity appears to be of minor significance for inosine metabolism. (iii) The utilization of exogenous purine deoxyribonucleosides is entirely dependent on a functional purine nucleoside phosphorylase. (iv) The pathway by which exogenous adenine is converted to guanine nucleotides in the presence of histidine requires a functional purine nucleoside phosphorylase. Evidence is presented that this pathway involves the conversion of adenine to adenosine, followed by deamination to inosine and subsequent phosphorolysis to hypoxanthine. Hypoxanthine is then converted to inosine monophosphate by inosine monophosphate pyrophosphorylase. The rate-limiting step in this pathway is the synthesis of adenosine from adenine due to lack of endogenous ribose-l-phosphate.
D000225:Adenine / Q000378:metabolism; D000619:Aminohydrolases / Q000378:metabolism; D002247:Carbon Isotopes; D003470:Culture Media; D006147:Guanine / Q000378:metabolism; D006639:Histidine / Q000378:metabolism; D007042:Hypoxanthines / Q000096:biosynthesis / Q000378:metabolism; D009154:Mutation; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D009705:Nucleosides / Q000378:metabolism*; D009711:Nucleotides / Q000096:biosynthesis; D010710:Phosphates / Q000378:metabolism; D010759:Phosphorus Isotopes; D010770:Phosphotransferases / Q000378:metabolism; D011687:Purines / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D013053:Spectrophotometry; D014161:Transduction, Genetic; D014166:Transferases / Q000378:metabolism
D002247:Carbon Isotopes; D003470:Culture Media; D007042:Hypoxanthines; D009705:Nucleosides; D009711:Nucleotides; D010710:Phosphates; D010759:Phosphorus Isotopes; D011687:Purines; D006639:Histidine; D006147:Guanine; D014166:Transferases; D010770:Phosphotransferases; D009699:N-Glycosyl Hydrolases; D000619:Aminohydrolases; D000225:Adenine