title: Polysome turnover during amino acid starvation in Escherichia coli.
abstract: The experiments presented in this paper support earlier evidence that ribosomes are released from polysomes when they encounter a codon for which no charged transfer ribonucleic acid is available. However, it is further shown that these ribosomes then reinitiate and resume translation. The size and the level of polysomes during deprival of an amino acid is a function of the frequency with which that particular amino acid appears in cellular proteins. Polysomes from starved cells are more stable than those from growing cells, and, moreover, polysomes from starved relaxed strains are more stable than those from starved stringent strains.
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D003470:Culture Media; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D005827:Genetics, Microbial; D009154:Mutation; D010452:Peptide Biosynthesis; D012329:RNA, Bacterial / Q000096:biosynthesis; D012333:RNA, Messenger; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D012293:Rifampin / Q000494:pharmacology; D013053:Spectrophotometry; D013395:Sucrose; D014316:Tritium; D014465:Ultrasonics; D014732:Vibration
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D002247:Carbon Isotopes; D003470:Culture Media; D012329:RNA, Bacterial; D012333:RNA, Messenger; D014316:Tritium; D013395:Sucrose; D012293:Rifampin