title: Role of exonuclease and beta protein of phage lambda in genetic recombination. V. Recombination of lambda DNA in vitro.
abstract: The sequential action of lambda exonuclease and polynucleotide ligase upon redundant joint molecules is sufficient to produce intact polynucleotide chains and heat-stable, biologically active molecules of lambda DNA, whereas the action of ligase alone is insufficient. These results (a) confirm the previously described mechanism of single-strand assimilation, including a subsidiary mechanism by which the further action of lambda exonuclease is arrested when a redundant strand is completely assimilated, and (b) represent a simulation of the steps in genetic recombination that follow the formation of biparental complexes (synapsis). lambda exonuclease is postulated to catalyze a concerted reaction that includes exposure of complementary sequences, formation of heteroduplex regions, and elimination of redundant branches.
mesh_terms: D001681:Biological Assay; D002848:Chromatography, DEAE-Cellulose; D003090:Coliphages / Q000201:enzymology; D004279:DNA, Viral; D003851:Deoxyribonucleases; D004926:Escherichia coli; D005827:Genetics, Microbial; D008025:Ligases; D010759:Phosphorus Isotopes; D011119:Polynucleotides; D012343:RNA, Transfer; D011995:Recombination, Genetic; D014316:Tritium
chemical_list: D004279:DNA, Viral; D010759:Phosphorus Isotopes; D011119:Polynucleotides; D014316:Tritium; D012343:RNA, Transfer; D003851:Deoxyribonucleases; D008025:Ligases