Membrane association of conjugally transferred deoxyribonucleic acid in Escherichia coli minicells.
The conjugally acquired deoxyribonucleic acid (DNA) of small, anucleate cells ("minicells") of a mutant strain of Escherichia coli K-12 was found to be predominantly associated with the bacterial membrane. Evidence from X-irradiation studies in vivo shows that there is no decrease in DNA-membrane association under conditions which reduce the DNA to one-sixth its original size and suggests the possibility of multiple DNA-membrane association sites. Preliminary enzymatic studies indicate the involvement of protein, DNA, and lipids in the membrane association of the DNA.
D001665:Binding Sites; D002247:Carbon Isotopes; D002462:Cell Membrane; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D003090:Coliphages; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000528:radiation effects; D003851:Deoxyribonucleases / Q000494:pharmacology; D003902:Detergents; D004926:Escherichia coli / Q000254:growth & development / Q000528:radiation effects; D007930:Leucine / Q000378:metabolism; D008049:Lipase / Q000494:pharmacology; D008970:Molecular Weight; D009154:Mutation; D010447:Peptide Hydrolases / Q000494:pharmacology; D011830:Radiation Effects; D013395:Sucrose; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014364:Tryptophan / Q000378:metabolism
D002247:Carbon Isotopes; D002712:Chlorides; D004269:DNA, Bacterial; D003902:Detergents; D014316:Tritium; D002586:Cesium; D013395:Sucrose; D014364:Tryptophan; D003851:Deoxyribonucleases; D008049:Lipase; D010447:Peptide Hydrolases; D007930:Leucine; D013936:Thymidine