T-even bacteriophage-tolerant mutants of Escherichia coli B. I. Isolation and preliminary characterization.
A general procedure is described for isolation of T-even phage-tolerant mutants of Escherichia coli. Two such mutants of E. coli B have been examined in some detail. These mutants adsorb T-even phages but are unable to release viable progeny. Under certain conditions, viability of the cells is completely unaffected by phage infection in one mutant, and there is but a slight decrease in colony-forming ability in the other. Phage deoxyribonucleic acid (DNA) is injected into these cells, as shown by the formation of phage-specific enzymes, but it is not degraded to acid-soluble material. Some phage DNA replication occurs in both strains. The mutants are both more resistant to ultraviolet light than is the parent strain.
D000327:Adsorption; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D003090:Coliphages / Q000254:growth & development* / Q000378:metabolism; D003470:Culture Media; D004261:DNA Replication; D004279:DNA, Viral / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification* / Q000528:radiation effects; D005827:Genetics, Microbial; D006146:Guanidines / Q000494:pharmacology; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation; D009603:Nitroso Compounds / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014466:Ultraviolet Rays; D014498:Uracil / Q000378:metabolism; D014779:Virus Replication
D002247:Carbon Isotopes; D002712:Chlorides; D003470:Culture Media; D004279:DNA, Viral; D006146:Guanidines; D009153:Mutagens; D009603:Nitroso Compounds; D014316:Tritium; D002586:Cesium; D014498:Uracil; D013936:Thymidine