title: The problems of large scale extraction and purification of microbial enzymes.
mesh_terms: D001431:Bacteriological Techniques; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004798:Enzymes / Q000302:isolation & purification*; D004926:Escherichia coli / Q000201:enzymology; D005374:Filtration; D008025:Ligases / Q000302:isolation & purification; D012343:RNA, Transfer; D012440:Saccharomyces / Q000201:enzymology; D013678:Technology, Pharmaceutical
chemical_list: D004798:Enzymes; D012343:RNA, Transfer; D008025:Ligases