Slow joining of newly replicated DNA chains in DNA polymerase I-deficient Escherichia coli mutants.
In Escherichia coli mutants deficient in DNA polymerase I, newly replicated short DNA is joined at about 10% of the rate in the wild-type strains. It is postulated that DNA polymerase I normally functions in filling gaps between the nascent short segments synthesized by the replication complex. Possible implications of the finding are discussed in relation to other abnormal properties of these mutants.
D001665:Binding Sites; D002499:Centrifugation, Density Gradient; D003470:Culture Media; D004254:DNA Nucleotidyltransferases / Q000494:pharmacology*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis*; D003903:Deuterium; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008025:Ligases / Q000378:metabolism; D009154:Mutation; D009587:Nitrogen Isotopes; D011485:Protein Binding; D013936:Thymidine; D013941:Thymine; D014316:Tritium
D003470:Culture Media; D004269:DNA, Bacterial; D009587:Nitrogen Isotopes; D014316:Tritium; D003903:Deuterium; D004254:DNA Nucleotidyltransferases; D008025:Ligases; D013941:Thymine; D013936:Thymidine