Mitomycin C and temperature induction of colicin B in the absence of deoxyribonucleic acid synthesis.
An Escherichia coli K-12 strain and its mutant, temperature-sensitive in deoxyribonucleic acid (DNA) synthesis, were used as hosts for two different ColB factors (ColB3-K166 and ColB4-K98). Induction of either colicin occurred in both hosts in the presence of mitomycin C at 42 C. Induction of the temperature-sensitive colicinogenic hosts also occurred without mitomycin C at 42 C at which temperature no DNA synthesis was observed. Colicin synthesis was a nonlethal event in ColB4-K98(+) cultures, whereas in cultures of ColB3-K166(+) induction led to death of a large fraction of the population.
D003087:Colicins / Q000096:biosynthesis*; D004269:DNA, Bacterial / Q000096:biosynthesis*; D003839:Deoxyadenosines / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D005827:Genetics, Microbial; D008937:Mitomycins / Q000494:pharmacology*; D009154:Mutation; D013696:Temperature; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium
D003087:Colicins; D004269:DNA, Bacterial; D003839:Deoxyadenosines; D008937:Mitomycins; D014316:Tritium; D013936:Thymidine