T3 and T7 bacteriophage deoxyribonucleic acid-directed enzyme synthesis in vitro.
The T3 phage enzymes S-adenosyl methionine cleaving enzyme and lysozyme and the T7 lysozyme were synthesized in a deoxyribonucleic acid (DNA)-dependent, cell-free system derived from uninfected Escherichia coli. The data presented suggest that these enzymes are encoded in that portion of the DNA which is transcribed early after infection.
D002247:Carbon Isotopes; D002474:Cell-Free System; D002855:Chromatography, Thin Layer; D003090:Coliphages / Q000201:enzymology*; D004279:DNA, Viral; D004926:Escherichia coli; D005815:Genetic Code; D005827:Genetics, Microbial; D008715:Methionine / Q000378:metabolism; D009113:Muramidase / Q000096:biosynthesis*; D014166:Transferases / Q000378:metabolism*
D002247:Carbon Isotopes; D004279:DNA, Viral; D008715:Methionine; D014166:Transferases; D009113:Muramidase