title: Cross-linked transfer RNA functions in all steps of the translation process.
abstract: A specific photochemical reaction between 4-thiouridine and cytosine cross-links two arms of transfer RNA. This cross-link, introduced into phenylalanine transfer RNA and arginine transfer RNA, limits the conformational freedom of the molecule. Both modified transfer RNA's are capable of functioning in all steps of protein synthesis with this restraint on allowable conformations.
mesh_terms: D001120:Arginine / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis; D002247:Carbon Isotopes; D004926:Escherichia coli; D005815:Genetic Code; D009705:Nucleosides; D010649:Phenylalanine / Q000378:metabolism; D010777:Photochemistry; D012343:RNA, Transfer
chemical_list: D001426:Bacterial Proteins; D002247:Carbon Isotopes; D009705:Nucleosides; D010649:Phenylalanine; D012343:RNA, Transfer; D001120:Arginine