Identification of Mycoplasmatales: procedures for both characterization and purification.
Procedures with potential value for identification and purification of Mycoplasma were applied to over 240 cultures representing 11 of the 12 known avian serotypes. Growth was tested at pH 5.5 and 9.5, at 25 and 42 C, in serum-free media, and in the presence of (i) 1% bile salts, (ii) 3% NaCl, and (iii) 0.02% methylene blue. One avian Mycoplasma serotype grew in broth containing 1% bile salts. Two of 11 avian serotypes were resistant to 0.02% methylene blue. A number of Mycoplasma strains grew at 42 C or in a pH 9.5 medium. Usefulness of these procedures for purifying cultures of Mycoplasma is discussed.
D000362:Agar; D000438:Alcohols; D000818:Animals; D001386:Azides / Q000494:pharmacology; D001431:Bacteriological Techniques; D001647:Bile Acids and Salts / Q000494:pharmacology; D002122:Calcium Chloride / Q000494:pharmacology; D003470:Culture Media; D004352:Drug Resistance, Microbial; D004917:Erythromycin / Q000494:pharmacology; D005840:Gentian Violet / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera; D007612:Kanamycin / Q000494:pharmacology; D008460:Meat; D008722:Methods; D008751:Methylene Blue / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D009174:Mycoplasma / Q000145:classification* / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D011817:Rabbits; D012703:Serotyping; D012965:Sodium Chloride / Q000494:pharmacology; D013552:Swine; D013696:Temperature
D000438:Alcohols; D001386:Azides; D001647:Bile Acids and Salts; D003470:Culture Media; D007106:Immune Sera; D012965:Sodium Chloride; D007612:Kanamycin; D004917:Erythromycin; D000362:Agar; D005840:Gentian Violet; D002122:Calcium Chloride; D008751:Methylene Blue