The preparation of an alkali-soluble collagen from demineralized bone.
Ossein was solubilized by the action of alkali and a resulting high-molecular-weight fraction isolated. The chemical and physical properties of this fraction were studied and compared with those of an acid-soluble collagen prepared from calf skin by conventional techniques. From the results it is concluded that the alkali-soluble protein exhibits only minor differences from acid-soluble collagen, and that these differences can be ascribed for the most part to a decrease in the inter- and intramolecular cross-linking.
D000143:Acids; D000468:Alkalies; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001842:Bone and Bones / Q000032:analysis*; D002417:Cattle; D003094:Collagen / Q000032:analysis / Q000302:isolation & purification*; D003648:Decalcification Technique; D004589:Electrophoresis, Disc; D008854:Microscopy, Electron; D008970:Molecular Weight; D009904:Optical Rotation; D012867:Skin / Q000032:analysis; D012995:Solubility; D013329:Structure-Activity Relationship; D014461:Ultracentrifugation; D014783:Viscosity
D000143:Acids; D000468:Alkalies; D000596:Amino Acids; D003094:Collagen