An endonuclease activity from Escherichia coli absent from certain rec- strains.
A new endonuclease activity from Escherichia coli which cleaves circular, single-stranded DNA in the presence of added nucleoside triphosphate has been purified. The activity has not been detected in extracts from certain rec(-) strains and is therefore implicated in genetic recombination.
D000255:Adenosine Triphosphate; D002499:Centrifugation, Density Gradient; D003090:Coliphages; D004269:DNA, Bacterial; D004279:DNA, Viral; D003851:Deoxyribonucleases / Q000302:isolation & purification*; D004151:Dipeptides; D004926:Escherichia coli / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D008274:Magnesium; D009154:Mutation; D009711:Nucleotides; D011995:Recombination, Genetic
D004269:DNA, Bacterial; D004279:DNA, Viral; D004151:Dipeptides; D009711:Nucleotides; D000255:Adenosine Triphosphate; D003851:Deoxyribonucleases; D008274:Magnesium