Physicochemical and light scattering studies on ribosome particles.
The light scattering technique has been used to measure the molecular weight of Escherichia coli ribosomes. The 30S, 50S, and 70S components have been isolated and purified. The refractive index increment dn/dc was found to have the same value, (0.20 +/-0.01) cm(3)/g, for the three species. The molecular weights are (1.0 +/-0.1).10(6), (1.7 +/-0.1).10(6), and (2.9 +/-0.3).10(6) daltons respectively. Some information about the dimensions in solution (radius of gyration) and the interaction constant (second virial coefficient) have been obtained, and their significance is discussed.
D002021:Buffers; D004926:Escherichia coli / Q000166:cytology; D008027:Light; D008433:Mathematics; D008722:Methods; D008970:Molecular Weight; D010179:Pancreas / Q000201:enzymology; D011506:Proteins / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D012260:Ribonucleases; D012270:Ribosomes / Q000032:analysis; D012542:Scattering, Radiation; D013395:Sucrose; D014461:Ultracentrifugation
D002021:Buffers; D011506:Proteins; D012367:RNA, Viral; D013395:Sucrose; D012260:Ribonucleases