Chemical modifications of transfer RNA species. Desulfurization with Raney nickel.
A single, modified nucleoside in Escherichia coli tRNA, 6-(3-methyl-2-butenylamino)-2-methyl-thio-9-beta-D-ribofuranosylpurine, has been desulfurized with Raney nickel to afford its probable biosynthetic precursor. The limitations of the reaction at the nucleoside and tRNA levels and its lack of inhibition of the amino acid acceptor activity of tRNA are described.
D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D002854:Chromatography, Paper; D004926:Escherichia coli / Q000378:metabolism*; D009532:Nickel; D009705:Nucleosides / Q000032:analysis; D010649:Phenylalanine / Q000378:metabolism; D010937:Plant Growth Regulators; D012329:RNA, Bacterial / Q000096:biosynthesis; D012343:RNA, Transfer / Q000096:biosynthesis; D012694:Serine / Q000378:metabolism; D013057:Spectrum Analysis; D013455:Sulfur; D014443:Tyrosine / Q000378:metabolism
D009705:Nucleosides; D010937:Plant Growth Regulators; D012329:RNA, Bacterial; D014443:Tyrosine; D012694:Serine; D010649:Phenylalanine; D013455:Sulfur; D009532:Nickel; D012343:RNA, Transfer