Reversal of the antimicrobial activity of trimethoprim by thymidine in commercially prepared media.
The ability of a potent dihydrofolate reductase inhibitor, trimethoprim, to inhibit the growth of Escherichia coli B in vitro is dependent on the composition of the medium in which the cells are grown. The inhibition observed in minimal broth could be partially reversed by the addition of thymidine, ribonucleosides, amino acids, and vitamins. No reversal occurred in the absence of thymidine. In a number of commercially prepared media, the inhibitory activity of trimethoprim correlated inversely with the amount of thymidine found to be present by microbiological assay. The significance of these findings for the routine testing of new, synthetic antibacterial agents is discussed.
D000596:Amino Acids / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001681:Biological Assay; D003470:Culture Media; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D005493:Folic Acid Antagonists / Q000494:pharmacology*; D007778:Lactobacillus; D011743:Pyrimidines / Q000494:pharmacology*; D012263:Ribonucleosides / Q000378:metabolism; D013936:Thymidine / Q000032:analysis / Q000378:metabolism*; D014815:Vitamins / Q000378:metabolism
D000596:Amino Acids; D000900:Anti-Bacterial Agents; D003470:Culture Media; D005493:Folic Acid Antagonists; D011743:Pyrimidines; D012263:Ribonucleosides; D014815:Vitamins; D013936:Thymidine