R-B enteric differential system for identification of Enterobacteriaceae.
The R/B Enteric Differential System for identifying enteric bacteria has been evaluated with 451 "unknown" cultures from the stock culture collection of the Center for Disease Control. An average of 89.6% of these cultures were correctly identified by the R/B system, when used as recommended by the manufacturer but without the assistance of serology. This percentage ranged, however, from 47% for Klebsiella to 100% for Serratia and Providencia. Of 11 groups or genera of Enterobacteriaceae tested, only three (Enterobacter, Serratia, and Providencia) were identified with 95% or better accuracy. Four groups (Arizona, Citrobacter, Escherichia, and Salmonella) attained 90 to 95% accuracy of identification, and three groups (Edwardsiella, Proteus, and Shigella) scored between 85 and 90% accuracy. We recommend the R/B system as a screening device which is reasonably successful in grouping bacteria but not as a substitute for more exacting conventional procedures.
D001431:Bacteriological Techniques; D002262:Carboxy-Lyases / Q000378:metabolism; D002465:Cell Movement; D003470:Culture Media; D004755:Enterobacteriaceae / Q000145:classification* / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism; D005069:Evaluation Studies as Topic; D005740:Gases / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism; D006859:Hydrogen / Q000096:biosynthesis; D007211:Indoles / Q000096:biosynthesis; D008190:Lyases / Q000378:metabolism; D008239:Lysine; D009952:Ornithine; D010649:Phenylalanine; D013440:Sulfides / Q000096:biosynthesis
D003470:Culture Media; D005740:Gases; D007211:Indoles; D013440:Sulfides; D010649:Phenylalanine; D006859:Hydrogen; D009952:Ornithine; D008190:Lyases; D002262:Carboxy-Lyases; D005947:Glucose; D008239:Lysine