Acetyl CoA carboxylase: the purified transcarboxylase component.
Acetyl CoA carboxylase of Escherichia coli has been resolved into three functionally dissimilar proteins: (1) biotin-carboxyl carrier protein (BCCP); (2) a biotin carboxylase component that catalyzes the Mn-ATP-dependent carboxylation of BCCP to form CO(2) (-)-BCCP; and (3) a transcarboxylase component that catalyzes the transfer of the carboxyl group from CO(2) (-)-BCCP to acetyl CoA to form malonyl CoA.The transcarboxylase has been purified 1700-fold. Evidence that this protein catalyzes the transcarboxylase step includes the demonstration that it (a) catalyzes the carboxylation of BCCP, (b) catalyzes the BCCP-dependent exchange between [(14)C]acetyl CoA and malonyl CoA, (c) binds labeled acetyl CoA and malonyl CoA, and (d) catalyzes the decarboxylation of CO(2) (-)-BCCP. On the basis of this evidence, it is concluded that the transcarboxylase component contains sites for the acyl CoA group and for biotin, the covalently bound prosthetic group of BCCP.
D001345:Autoradiography; D001710:Biotin / Q000032:analysis; D002247:Carbon Isotopes; D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D003065:Coenzyme A / Q000032:analysis*; D004926:Escherichia coli / Q000201:enzymology; D008025:Ligases / Q000494:pharmacology; D008314:Malonates; D011485:Protein Binding; D011506:Proteins / Q000032:analysis; D011773:Pyruvates; D014166:Transferases / Q000302:isolation & purification*; D014316:Tritium
D002247:Carbon Isotopes; D008314:Malonates; D011506:Proteins; D011773:Pyruvates; D014316:Tritium; D001710:Biotin; D014166:Transferases; D008025:Ligases; D003065:Coenzyme A