Effect of bacteriophage R17 infection on host-directed synthesis of ribosomal ribonucleates.
Studies were performed on the synthesis of ribosomal ribonucleates in cells of Escherichia coli K-12 infected by the ribonucleic acid (RNA) bacteriophage R17. Host-specific RNA was measured in the presence of phage RNA by in vitro hybridization of the purified ribonucleates with E. coli deoxyribonucleic acid. The results showed that, although the overall rate of RNA synthesis was only slightly affected by phage infection, the level of host RNA synthesis was decreased by 70 to 80%. Fractionation of the purified ribonucleates by sucrose gradient sedimentation, followed by hybridization of fractions sedimenting in the 23S and 16S regions, revealed that the level of ribosomal RNA synthesis was also decreased by 70 to 80%, and that this inhibition occurred during the first 15 to 20 min after infection. These findings are discussed in light of what is known about the inhibition of host RNA synthesis by other virus systems.
D002499:Centrifugation, Density Gradient; D003090:Coliphages / Q000302:isolation & purification / Q000378:metabolism*; D004269:DNA, Bacterial / Q000302:isolation & purification; D004926:Escherichia coli / Q000378:metabolism*; D006824:Hybridization, Genetic; D007700:Kinetics; D010759:Phosphorus Isotopes; D012329:RNA, Bacterial / Q000096:biosynthesis*; D012367:RNA, Viral / Q000302:isolation & purification; D012270:Ribosomes / Q000378:metabolism*; D014316:Tritium
D004269:DNA, Bacterial; D010759:Phosphorus Isotopes; D012329:RNA, Bacterial; D012367:RNA, Viral; D014316:Tritium