Crystallization and preliminary structural analysis of dihydrolipoyl transsuccinylase, the core of the 2-oxoglutarate dehydrogenase complex.
Dihydrolipoyl transsuccinylase, one of the three enzymes comprising the Escherichia coli 2-oxoglutarate dehydrogenase (EC 1.2.4.2) complex, has been crystallized. Studies by x-ray diffraction and electron microscopy establish that the transsuccinylase has octahedral (432) symmetry, i.e., it consists of 24 subunits that are structurally identical.
D003460:Crystallization; D004926:Escherichia coli / Q000201:enzymology; D005977:Glutarates / Q000378:metabolism; D008854:Microscopy, Electron; D010088:Oxidoreductases / Q000032:analysis*; D010781:Photography; D011773:Pyruvates / Q000378:metabolism; D013386:Succinates; D014166:Transferases / Q000032:analysis*; D014961:X-Ray Diffraction
D005977:Glutarates; D011773:Pyruvates; D013386:Succinates; D010088:Oxidoreductases; D014166:Transferases