Alterations in the cytoplasmic membrane proteins of various chlorate-resistant mutants of Escherichia coli.
Chlorate-resistant mutants corresponding to each known genetic locus (chlA, chlB, chlC, chlD, chlE) were isolated from Escherichia coli K-12. All these mutants showed decreased amounts of membrane-bound nitrate reductase, cytochrome b, and formic dehydrogenase, but all had normal succinic dehydrogenase activity. Proteins from the cytoplasmic membranes of these mutants were compared to those of the wild type-on polyacrylamide gels. The addition of nitrate to wild-type anaerobic cultures caused increased formation of three membrane proteins. These same proteins, along with one other, were missing in varying patterns in mutants altered at the different genetic loci. One of the missing proteins was found to be the enzyme nitrate reductase, although this protein was present in some mutants lacking nitrate reductase activity. None of the others has been identified.
D000693:Anaerobiosis; D001426:Bacterial Proteins; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002704:Chlorates / Q000494:pharmacology; D002713:Chlorine / Q000494:pharmacology*; D002874:Chromosome Mapping; D003470:Culture Media; D003580:Cytochromes / Q000378:metabolism; D004352:Drug Resistance, Microbial; D004589:Electrophoresis, Disc; D004926:Escherichia coli / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D005561:Formates; D007930:Leucine / Q000378:metabolism; D009154:Mutation; D009566:Nitrates; D010088:Oxidoreductases / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism; D014161:Transduction, Genetic; D014316:Tritium; D014443:Tyrosine / Q000378:metabolism
D001426:Bacterial Proteins; D002704:Chlorates; D003470:Culture Media; D003580:Cytochromes; D005561:Formates; D009566:Nitrates; D014316:Tritium; D014443:Tyrosine; D002713:Chlorine; D010088:Oxidoreductases; D013385:Succinate Dehydrogenase; D007930:Leucine