title: Inhibition of arginyl-transfer ribonucleic acid synthetase activity of Escherichia coli by arginine biosynthetic precursors.
abstract: The arginine biosynthetic precursors, ornithine, citrulline, and argininosuccinate, inhibit arginyl-transfer ribonucleic acid (tRNA) synthetase (EC 6.1.1.13, arginine: soluble RNA ligase, adenosine monophosphate) activity in the in vitro attachment assay system. Ornithine is the most potent, argininosuccinate is next, and citrulline is least effective. The implications of these results are discussed in relation to arginyl-tRNA synthetase activity and the level of the arginine biosynthetic enzymes during conditions of restricted and unrestricted supply of arginine to cells.
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D001120:Arginine / Q000096:biosynthesis / Q000378:metabolism / Q000494:pharmacology*; D002247:Carbon Isotopes; D002474:Cell-Free System; D002956:Citrulline / Q000494:pharmacology*; D003124:Colorimetry; D003470:Culture Media; D004794:Enzyme Repression; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D009952:Ornithine / Q000494:pharmacology*; D013053:Spectrophotometry; D013386:Succinates / Q000494:pharmacology
chemical_list: D002247:Carbon Isotopes; D003470:Culture Media; D013386:Succinates; D002956:Citrulline; D001120:Arginine; D009952:Ornithine; D000604:Amino Acyl-tRNA Synthetases