Differential recovery of auxotrophs after penicillin enrichment in Escherichia coli.
Various auxotrophs are recovered from a penicillin enrichment cycle with differing efficiencies. Reconstruction experiments indicate that, under starvation conditions in the presence of penicillin, most auxotrophs undergo some death, whereas prolineless mutants are virtually immune to penicillin-induced killing.
D001431:Bacteriological Techniques; D003470:Culture Media; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D005796:Genes; D005827:Genetics, Microbial; D006982:Hypertonic Solutions; D007038:Hypotonic Solutions; D007930:Leucine / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D009154:Mutation; D010400:Penicillin G / Q000494:pharmacology*; D011392:Proline / Q000378:metabolism
D003470:Culture Media; D006982:Hypertonic Solutions; D007038:Hypotonic Solutions; D011392:Proline; D008715:Methionine; D007930:Leucine; D010400:Penicillin G