Mapping of a human genetic regulator element by somatic cell genetic analysis.
The ES-2 esterase is a kidney-associated esterase and is expressed in a cloned mouse renal adenocarcinoma cell line. Somatic cell hybridization of the mouse renal adenocarcinoma with either mouse or human fibroblasts leads to the marked reduction of ES-2 esterase titer. The extinction of ES-2 esterase activity in mousexhuman hybrids is reversible. Extinction correlates with the presence of the human C(10) chromosome in the somatic cell hybrids; whereas reexpression of the ES-2 esterase is observed in hybrids which have lost C(10). Thus, the proposed regulator element involved is probably structurally linked to C(10); however, linkage to a member of the D or G groups cannot be completely excluded.
D000230:Adenocarcinoma; D000818:Animals; D002460:Cell Line; D002855:Chromatography, Thin Layer; D002906:Chromosomes, Human, 6-12 and X; D004950:Esterases / Q000378:metabolism*; D005347:Fibroblasts; D005809:Genes, Regulator; D006801:Humans; D006824:Hybridization, Genetic; D066298:In Vitro Techniques; D007621:Karyotyping; D007668:Kidney / Q000201:enzymology*; D008168:Lung; D051379:Mice; D008858:Microscopy, Phase-Contrast
D004950:Esterases