Use of yeast populations fractionated by zonal centrifugation to study the cell cycle.
Zonal centrifugation in a sucrose density gradient was used to separate yeast cells primarily by size and thus by age in the cell cycle. This approach provides an alternative to synchronous growth for examining the properties of cells at different stages in the cell cycle.
D002455:Cell Division; D002499:Centrifugation, Density Gradient; D002501:Centrifugation, Zonal; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D005696:Galactosidases / Q000096:biosynthesis; D005959:Glucosidases / Q000096:biosynthesis; D009952:Ornithine; D012313:RNA / Q000096:biosynthesis; D012440:Saccharomyces / Q000166:cytology / Q000201:enzymology / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D013395:Sucrose; D000637:Transaminases / Q000096:biosynthesis
D003470:Culture Media; D013395:Sucrose; D012313:RNA; D004247:DNA; D009952:Ornithine; D000637:Transaminases; D005696:Galactosidases; D005959:Glucosidases