title: Growth rate of Escherichia coli at elevated temperatures: limitation by methionine.
abstract: When Escherichia coli growing in minimal medium is shifted from 37 C to any temperature in the range 40 to 45 C, the growth rate immediately assumes a new, lower value, characteristic of that temperature. The decrease is shown to be due, in several strains, to decreased activity of the first enzyme of the methionine pathway, homoserine trans-succinylase, which thus appears to be more heat-sensitive than any other essential enzyme in the cell. This sensitivity does not involve progressive denaturation of the enzyme; rather, the response to a shift of temperature, in either direction, is immediate and reversible.
mesh_terms: D000217:Acyltransferases / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis; D002247:Carbon Isotopes; D002470:Cell Survival; D003470:Culture Media; D003540:Cystathionine / Q000096:biosynthesis; D004269:DNA, Bacterial / Q000096:biosynthesis; D004926:Escherichia coli / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism; D005827:Genetics, Microbial; D006714:Homoserine / Q000378:metabolism; D006358:Hot Temperature; D012695:L-Serine Dehydratase / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D008715:Methionine / Q000096:biosynthesis / Q000378:metabolism*; D009154:Mutation; D012329:RNA, Bacterial / Q000096:biosynthesis; D013386:Succinates; D014498:Uracil / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002247:Carbon Isotopes; D003470:Culture Media; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D013386:Succinates; D003540:Cystathionine; D014498:Uracil; D006714:Homoserine; D008715:Methionine; D000217:Acyltransferases; D012695:L-Serine Dehydratase; D007930:Leucine