A convenient solid-state substrate for assays of polynucleotide ligase: the poly(dA)-(poly(( 3 H)dT)-poly(dT))-cellulose.
D000227:Adenine Nucleotides; D000818:Animals; D002417:Cattle; D002460:Cell Line / Q000201:enzymology; D002482:Cellulose; D055598:Chemical Phenomena; D002621:Chemistry; D002712:Chlorides; D003035:Cobalt; D003090:Coliphages; D003854:Deoxyribonucleotides; D004789:Enzyme Activation; D004926:Escherichia coli / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D008025:Ligases / Q000032:analysis*; D008722:Methods; D008954:Models, Biological; D009693:Nucleic Acid Hybridization; D009713:Nucleotidyltransferases; D011119:Polynucleotides; D013942:Thymine Nucleotides; D013950:Thymus Gland / Q000201:enzymology; D014316:Tritium
D000227:Adenine Nucleotides; D002712:Chlorides; D003854:Deoxyribonucleotides; D011119:Polynucleotides; D013942:Thymine Nucleotides; D014316:Tritium; D003035:Cobalt; D002482:Cellulose; D009713:Nucleotidyltransferases; D008025:Ligases