title: Enzyme alterations in tyrosine and phenylalanine auxotrophs of Salmonella typhimurium.
abstract: The enzyme activities specified by the tyrA and pheA genes were studied in wildtype strain Salmonella typhimurium and in phenylalanine and tyrosine auxotrophs. As in Aerobacter aerogenes and Escherichia coli, the wild-type enzymes of Salmonella catalyze two consecutive reactions: chorismate --> prephenate --> 4-hydroxy-phenylpyruvate (tyrA), and chorismate --> prephenate --> phenylpyruvate (pheA). A group of tyrA mutants capable of interallelic complementation had altered enzymes which retained chorismate mutase T activity but lacked prephenate dehydrogenase. Similarly, pheA mutants (in which interallelic complementation does not occur) had one group with altered enzymes which retained chorismate mutase P but lacked prephenate dehydratase. Tyrosine and phenylalanine auxotrophs outside of these categories showed loss of both activities of their respective bifunctional enzyme. TyrA mutants which had mutase T were considerably derepressed in this activity by tyrosine starvation and consequently excreted prephenate. A new and specific procedure was developed for assaying prephenate dehydrogenase activity.
mesh_terms: D002474:Cell-Free System; D055598:Chemical Phenomena; D002621:Chemistry; D002848:Chromatography, DEAE-Cellulose; D003470:Culture Media; D003509:Cyclohexanecarboxylic Acids / Q000378:metabolism; D003510:Cyclohexanes; D004794:Enzyme Repression; D005816:Genetic Complementation Test; D005827:Genetics, Microbial; D006836:Hydro-Lyases / Q000378:metabolism*; D009154:Mutation; D010088:Oxidoreductases / Q000378:metabolism*; D010649:Phenylalanine / Q000378:metabolism*; D010667:Phenylpyruvic Acids / Q000096:biosynthesis; D010770:Phosphotransferases / Q000378:metabolism*; D011773:Pyruvates; D012486:Salmonella typhimurium / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D003470:Culture Media; D003509:Cyclohexanecarboxylic Acids; D003510:Cyclohexanes; D010667:Phenylpyruvic Acids; D011773:Pyruvates; D014443:Tyrosine; D010649:Phenylalanine; D010088:Oxidoreductases; D010770:Phosphotransferases; D006836:Hydro-Lyases