Selective associations of hormonal steroids with aminoacyl transfer RNAs and control of protein synthesis.
The hormonal steroids progesterone, estradiol, testosterone, and 5alpha-dihydrotestosterone bind to aminoacyl-tRNA, but not to deacylated tRNA, implying that a change in conformation of tRNA occurs on aminoacylation. Binding is restricted to a few tRNA species and depends on the structure of both tRNA and steroid. There is one binding site per aminoacyl-tRNA molecule, the specificity of which appears to depend on a restricted, single-stranded loop sequence and on the tRNA conformation. By binding to an aminoacyl-tRNA, a steroid can control polypeptide synthesis in a model in vitro system by inhibiting chain elongation under conditions where aminoacyl-tRNA concentration is rate-limiting.
D000215:Acylation; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D001665:Binding Sites; D002247:Carbon Isotopes; D004926:Escherichia coli / Q000032:analysis; D004958:Estradiol / Q000378:metabolism*; D008099:Liver / Q000032:analysis; D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000032:analysis; D010452:Peptide Biosynthesis; D010441:Peptide Chain Elongation, Translational; D010649:Phenylalanine / Q000378:metabolism; D011374:Progesterone / Q000378:metabolism* / Q000494:pharmacology; D011485:Protein Binding; D012343:RNA, Transfer / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D013739:Testosterone / Q000378:metabolism*; D014316:Tritium; D015003:Yeasts / Q000032:analysis
D000596:Amino Acids; D002247:Carbon Isotopes; D009711:Nucleotides; D014316:Tritium; D013739:Testosterone; D010649:Phenylalanine; D011374:Progesterone; D004958:Estradiol; D012343:RNA, Transfer