Valinomycin-induced uptake of potassium in membrane vesicles from Escherichia coli.
Osmotically shocked Escherichia coli and membrane vesicle ghosts from E. coli cells have lost the ability to accumulate potassium by active transport. The addition of valinomycin to the membrane ghosts restores the capacity to accumulate radioactive (42)K and (86)Rb by a temperature- and energy-dependent process. Membrane vesicles prepared from mutants of E. coli altered in potassium transport show defects in the valinomycin-stimulated accumulation of (42)K that are related to the defects in the intact cells.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001693:Biological Transport, Active / Q000187:drug effects*; D002458:Cell Fractionation; D002463:Cell Membrane Permeability / Q000187:drug effects*; D002586:Cesium / Q000494:pharmacology; D002712:Chlorides / Q000378:metabolism; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011189:Potassium Chloride / Q000378:metabolism*; D011194:Potassium Isotopes; D012413:Rubidium / Q000378:metabolism; D014634:Valinomycin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D002712:Chlorides; D011194:Potassium Isotopes; D002586:Cesium; D014634:Valinomycin; D011189:Potassium Chloride; D012413:Rubidium