Proteolytic reaction of mammalian spermatozoa on gelatin membranes.
The acrosomes of spermatozoa of several mammalian species show proteolytic activity when applied to fixed gelatin membranes. The technique permits continuous observation of the enzymatic reaction of an individual spermatozoon. Release of the enzyme occurs solely in the region of the acrosome, in a manner which is species-specific.
D000818:Animals; D002417:Cattle; D006224:Cricetinae; D005780:Gelatin / Q000378:metabolism*; D006168:Guinea Pigs; D006801:Humans; D008297:Male; D008566:Membranes; D051379:Mice; D008858:Microscopy, Phase-Contrast; D010447:Peptide Hydrolases / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D013094:Spermatozoa / Q000201:enzymology*
D005780:Gelatin; D010447:Peptide Hydrolases