Alkaline sucrose gradient sedimentation of chromosomal deoxyribonucleic acid from Escherichia coli PolA +  and PolA -  strains during thymine starvation.
Single-strand breaks were not detected in the deoxyribonucleic acid of Escherichia coli after thymine starvation for up to 180 min, even in a sensitive PolA(-) strain.
D001431:Bacteriological Techniques; D002499:Centrifugation, Density Gradient; D002876:Chromosomes, Bacterial / Q000032:analysis*; D003470:Culture Media; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004260:DNA Repair; D004269:DNA, Bacterial / Q000032:analysis*; D004926:Escherichia coli / Q000032:analysis* / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D009154:Mutation; D013395:Sucrose; D013936:Thymidine / Q000378:metabolism; D013941:Thymine / Q000378:metabolism*; D014161:Transduction, Genetic; D014316:Tritium
D003470:Culture Media; D004269:DNA, Bacterial; D014316:Tritium; D013395:Sucrose; D004254:DNA Nucleotidyltransferases; D013941:Thymine; D013936:Thymidine