Peptide chain termination with mammalian release factor.
We report a method for the in vitro study of peptide chain termination in mammals. A proteinaceous release factor has been isolated from rabbit reticulocyte extracts. This factor and a polyribonucleotide template containing the bases U and A stimulate the release of N-formylmethionine from mammalian [N-formylmethionyl-tRNA(f).ribosome] intermediates. Our studies suggest that UAA is a terminator codon for mammalian cells.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004926:Escherichia coli; D005815:Genetic Code; D066298:In Vitro Techniques; D008715:Methionine / Q000378:metabolism*; D010452:Peptide Biosynthesis; D011119:Polynucleotides; D011506:Proteins / Q000302:isolation & purification; D012343:RNA, Transfer; D011817:Rabbits; D012156:Reticulocytes; D012270:Ribosomes; D013698:Templates, Genetic; D014316:Tritium
D000900:Anti-Bacterial Agents; D011119:Polynucleotides; D011506:Proteins; D014316:Tritium; D012343:RNA, Transfer; D008715:Methionine