A complex between initiation factor IF2, guanosine triphosphate, and fMet-tRNA: an intermediate in initiation complex formation.
Evidence is presented that suggests the formation of a complex between polypeptide chain-initiation factor IF 2, GTP, and fMet-tRNA(f). This complex transfers both fMet-tRNA(f) and GTP to 30S ribosomal subunits in the presence of ApUpG and initiation factor IF 1. The resultant 30S initiation complex reacts with 50S subunits to form a 70S initiation complex. fMet-tRNA(f) in this 70S complex reacts with puromycin to form fMet-puromycin. These results suggest that [IF 2, GTP, fMet-tRNA(f)] is an intermediate in the initiation of protein synthesis in Escherichia coli.
D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis*; D001665:Binding Sites; D002850:Chromatography, Gel; D003597:Cytosine Nucleotides / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D006150:Guanine Nucleotides / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism*; D010442:Peptide Chain Initiation, Translational; D010649:Phenylalanine / Q000378:metabolism; D010759:Phosphorus Isotopes; D011119:Polynucleotides / Q000378:metabolism; D011485:Protein Binding; D011691:Puromycin / Q000494:pharmacology; D012329:RNA, Bacterial / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism; D014316:Tritium; D014500:Uracil Nucleotides / Q000378:metabolism
D001426:Bacterial Proteins; D003597:Cytosine Nucleotides; D006150:Guanine Nucleotides; D010759:Phosphorus Isotopes; D011119:Polynucleotides; D012329:RNA, Bacterial; D014500:Uracil Nucleotides; D014316:Tritium; D000249:Adenosine Monophosphate; D010649:Phenylalanine; D011691:Puromycin; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D012343:RNA, Transfer; D008715:Methionine