Compartmentalization of pseudorabies virus and subvirion components in BHK-21 cells and in the extracellular fluid.
The intracellular and extracellular localization of pseudorabies virions and subvirion components was determined at various stages in the replicative cycle. It was discovered that infectious pseudorabies virus appears first in the nucleus of the infected cell early in the infectious cycle but later accumulates in the cytoplasm. Subvirion components (nucleoids and nucleocapsids) are restricted to the nucleus, and only the complete virion is released from the infected cell.
D000818:Animals; D002247:Carbon Isotopes; D002460:Cell Line; D002467:Cell Nucleus; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D046508:Culture Techniques; D003593:Cytoplasm; D006462:Hemolytic Plaque Technique; D006564:Herpesviridae / Q000254:growth & development*; D007668:Kidney; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014779:Virus Replication
D002247:Carbon Isotopes; D014316:Tritium; D013936:Thymidine