title: Biochemical characterization of the ctr mutants of Escherichia coli.
abstract: A test procedure based on complementation in mixed extracts is described for the assay of heat-stable protein and enzyme I of the phosphoenolpyruvate-dependent phosphotransferase system. The test was used to assay a collection of pleiotropic carbohydrate mutants of Escherichia coli (ctr mutants) and revertants of these mutants. All mutants were found to lack enzyme I of the phosphoenolpyruvate-dependent transferase system. Revertants of these mutants to complete wild phenotype regained enzyme I-forming ability. Reversion to partial wild type was not accompanied by restoration of enzyme I-forming ability.
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis; D002474:Cell-Free System; D003470:Culture Media; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D005809:Genes, Regulator; D005816:Genetic Complementation Test; D005827:Genetics, Microbial; D006027:Glycosides / Q000378:metabolism; D009154:Mutation; D009596:Nitrophenols / Q000096:biosynthesis; D010085:Oxidative Phosphorylation; D010641:Phenotype; D010728:Phosphoenolpyruvate; D010770:Phosphotransferases / Q000378:metabolism*; D013053:Spectrophotometry
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D006027:Glycosides; D009596:Nitrophenols; D010728:Phosphoenolpyruvate; D010770:Phosphotransferases