Comparison of selective media for isolation of presumptive group D streptococci from human feces.
Pfizer Selective Enterococcus (PSE) agar, a medium containing bile, sodium azide, and esculin, was evaluated for its sensitivity and selectivity for detection and enumeration of presumptive group D streptococci in human feces. SF broth and SF broth plus agar (1.5%), representing selective media in common use, were studied simultaneously. Presumptive group D streptococci were recovered on PSE agar from the feces of all 25 subjects. No growth was observed in 8% of specimens in SF broth. No gram-negative organisms were recovered in any medium. PSE agar has the advantages of selecting out Streptococcus bovis, earlier appearance of distinctive reactions, and lack of requirement for special incubation temperature.
D000362:Agar; D000818:Animals; D001386:Azides; D001431:Bacteriological Techniques; D001646:Bile; D050260:Carbohydrate Metabolism; D002374:Catalase / Q000378:metabolism; D003470:Culture Media; D004912:Erythrocytes / Q000276:immunology; D005243:Feces / Q000382:microbiology*; D005285:Fermentation; D005419:Flavonoids; D005780:Gelatin / Q000378:metabolism; D006461:Hemolysis; D006736:Horses; D006801:Humans; D006868:Hydrolysis; D012703:Serotyping; D013045:Species Specificity; D013291:Streptococcus / Q000145:classification* / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism
D001386:Azides; D003470:Culture Media; D005419:Flavonoids; D005780:Gelatin; D000362:Agar; D002374:Catalase