title: Replication and recombination in ligase-deficient rII bacteriophage T4D.
abstract: Deoxyribonucleic acid replication and genetic recombination were investigated after infection of Escherichia coli with ligase-deficient rII bacteriophage T4D. The major observations are: (i) deoxyribonucleic acid synthesis is discontinuous, (ii) the discontinuities are more slowly repaired than in wild-type infection, (iii) host ligase is required for viability, and (iv) genetic recombination is increased.
mesh_terms: D002499:Centrifugation, Density Gradient; D002701:Chloramphenicol / Q000494:pharmacology; D002874:Chromosome Mapping; D003090:Coliphages / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D003433:Crosses, Genetic; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis; D004926:Escherichia coli / Q000201:enzymology; D005827:Genetics, Microbial; D008025:Ligases / Q000378:metabolism*; D009154:Mutation; D010759:Phosphorus Isotopes; D011119:Polynucleotides; D011995:Recombination, Genetic; D013395:Sucrose; D013489:Suppression, Genetic; D013696:Temperature; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D010759:Phosphorus Isotopes; D011119:Polynucleotides; D014316:Tritium; D013395:Sucrose; D002701:Chloramphenicol; D008025:Ligases; D013936:Thymidine