title: Enriched selection of dominant mutations: histidine operator mutations.
abstract: In the course of selection of bacteria with derepressed levels of histidine biosynthetic enzymes, it was found that when mutagen-treated cells were spread on a selective medium without allowing intervening growth to occur, the frequency of operator mutants obtained was dramatically increased. This may be useful as a general enrichment for operator or other dominant mutations.
mesh_terms: D000409:Alanine; D001431:Bacteriological Techniques; D003470:Culture Media; D005799:Genes, Dominant; D005809:Genes, Regulator; D005827:Genetics, Microbial; D005947:Glucose; D006639:Histidine / Q000096:biosynthesis*; D009153:Mutagens; D009154:Mutation; D012486:Salmonella typhimurium / Q000166:cytology / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D014230:Triazoles
chemical_list: D003470:Culture Media; D009153:Mutagens; D014230:Triazoles; D006639:Histidine; D005947:Glucose; D000409:Alanine