Comparison of two plating media for the isolation of Erysipelothrix rhusiopathiae from enrichment broth culture.
Sodium azide-crystal violet-agar and a modified blood-azide (MBA)-agar were used to isolate Erysipelothrix rhusiopathiae from swine tissues. There was no significant difference in the number of isolations. However, 78% of the isolants from modified blood-azide medium required only 24 hr of incubation, whereas all of the isolants obtained on sodium azide-crystal violet medium required 48 hr.
D000362:Agar; D000818:Animals; D001386:Azides; D001431:Bacteriological Techniques / Q000592:standards*; D001769:Blood; D002417:Cattle; D006691:Classical Swine Fever / Q000175:diagnosis; D003470:Culture Media; D003937:Diagnosis, Differential; D004888:Erysipelothrix / Q000302:isolation & purification*; D005840:Gentian Violet; D007668:Kidney / Q000382:microbiology; D008099:Liver / Q000382:microbiology; D014066:Palatine Tonsil / Q000382:microbiology; D012964:Sodium; D013154:Spleen / Q000382:microbiology; D013552:Swine; D013997:Time Factors
D001386:Azides; D003470:Culture Media; D000362:Agar; D012964:Sodium; D005840:Gentian Violet