Nucleic acid enzymology of extremely halophilic bacteria. Halobacterium cutirubrum deoxyribonucleic acid-dependent ribonucleic acid polymerase.
1. DNA-dependent RNA polymerase was purified 150-fold from crude extracts of the extreme halophile Halobacterium cutirubrum. 2. The enzyme requires the presence of native DNA and all four nucleoside triphosphates to incorporate (14)C-labelled nucleoside triphosphate into an acid-insoluble ribonuclease-sensitive product. 3. It has an absolute requirement for both Mn(2+) and Mg(2+). 4. The polymerase requires a high salt concentration for stability, but is markedly inhibited by univalent cations. 5. Its molecular weight is very low compared with that of Escherichia coli RNA polymerase.
D002247:Carbon Isotopes; D004247:DNA; D003956:Dialysis; D004926:Escherichia coli; D006217:Halobacterium / Q000201:enzymology*; D008274:Magnesium; D008345:Manganese; D008970:Molecular Weight; D010710:Phosphates / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D012316:RNA Nucleotidyltransferases / Q000302:isolation & purification*; D012329:RNA, Bacterial; D011874:Radiometry; D012965:Sodium Chloride; D013053:Spectrophotometry; D014316:Tritium; D014732:Vibration
D002247:Carbon Isotopes; D010710:Phosphates; D011506:Proteins; D012329:RNA, Bacterial; D014316:Tritium; D008345:Manganese; D012965:Sodium Chloride; D004247:DNA; D012316:RNA Nucleotidyltransferases; D008274:Magnesium