Structure of the DNA ligase-adenylate intermediate: lysine (epsilon-amino)-linked adenosine monophosphoramidate.
Proteolytic degradation of the Escherichia coli DNA ligase-adenylate intermediate releases adenosine 5'-monophosphate linked to the epsilon-amino group of lysine by a phosphoamide bond. Measurements of the rate of hydroxylaminolysis of the ligase-adenylate provide further support for a phosphoamide linkage in the native enzyme. Lysine (epsilon-amino)-linked adenosine monophosphoramidate has also been isolated from the T4 phage-induced ligase-adenylate intermediate. These results indicate that an initial step of the DNA ligase reaction consists of the nucleophilic attack of the epsilon-amino group of a lysine residue of the enzyme on the adenylyl phosphorus of DPN or ATP that leads to the formation of enzyme-bound lysine (epsilonamino)-linked adenosine monophosphoramidate.
D000227:Adenine Nucleotides / Q000378:metabolism*; D000249:Adenosine Monophosphate / Q000378:metabolism; D000577:Amides / Q000378:metabolism*; D000626:Aminopeptidases / Q000378:metabolism; D001345:Autoradiography; D002855:Chromatography, Thin Layer; D003090:Coliphages / Q000201:enzymology; D004247:DNA / Q000378:metabolism*; D004586:Electrophoresis; D004926:Escherichia coli / Q000201:enzymology; D006639:Histidine / Q000032:analysis; D006868:Hydrolysis; D006898:Hydroxylamines / Q000378:metabolism; D007292:Inosine Nucleotides / Q000032:analysis / Q000378:metabolism; D008025:Ligases / Q000378:metabolism*; D008239:Lysine / Q000032:analysis / Q000378:metabolism; D010759:Phosphorus Isotopes
D000227:Adenine Nucleotides; D000577:Amides; D006898:Hydroxylamines; D007292:Inosine Nucleotides; D010759:Phosphorus Isotopes; D000249:Adenosine Monophosphate; D006639:Histidine; D004247:DNA; D000626:Aminopeptidases; D008025:Ligases; D008239:Lysine