L-glutaminase: suppression of lymphocyte blastogenic responses in vitro.
Continuous exposure to 0.1 international unit or more of Escherichia coli L-glutaminase inhibited responses of human lymphocytes to phytohemagglutinin, streptolysin O, and allogeneic leukocytes. Inhibition was completety reversed by removing the enzyme from the culture or adding L-glutamine but not L-asparagine. Cytoxicity did not occur. L-Glutaminase should be immunosuppressive in vivo.
D046508:Culture Techniques; D004926:Escherichia coli / Q000201:enzymology; D005972:Glutaminase / Q000494:pharmacology*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D037102:Lectins / Q000494:pharmacology; D007962:Leukocytes; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects*; D013301:Streptolysins / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D007166:Immunosuppressive Agents; D037102:Lectins; D013301:Streptolysins; D014316:Tritium; D005972:Glutaminase; D013936:Thymidine