The role of the phosphoenolpyruvate phosphotransferase system in the transport of N-acetyl-D-glucosamine by Escherichia coli.
The properties of an N-acetyl-d-glucosamine-transport system have been studied by following the intracellular accumulation of methyl 2-acetamido-2-deoxy-alpha-d-[1-(14)C]glucoside by Escherichia coli. The same analogue was used to assay phosphoenolpyruvate phosphotransferase activity of toluene-treated cells. Transport and phosphorylation are induced by growth on d-glucosamine or N-acetyl-d-glucosamine. Mutants resistant to N-iodoacetyl-d-glucosamine are defective in the uptake and phosphorylation of the labelled glycoside.
D001693:Biological Transport, Active; D002247:Carbon Isotopes; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D005944:Glucosamine / Q000378:metabolism*; D005947:Glucose; D006027:Glycosides / Q000378:metabolism; D007461:Iodoacetates / Q000494:pharmacology; D009154:Mutation; D010756:Phosphoric Acids; D010770:Phosphotransferases / Q000378:metabolism*; D011773:Pyruvates; D014050:Toluene
D002247:Carbon Isotopes; D006027:Glycosides; D007461:Iodoacetates; D010756:Phosphoric Acids; D011773:Pyruvates; D014050:Toluene; D010770:Phosphotransferases; D005947:Glucose; D005944:Glucosamine