Elution of antibodies from biopsy tissue.
Cryostat sections proved to be sufficient material for acid elution of bound antibodies in vitro or in vivo. By single reconstitution of the pH the eluate may be studied for antibodies with the immunofluorescence technique. This micro-elution method may be of value for the study of kidney biopsy tissues.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000941:Antigens / Q000378:metabolism; D001706:Biopsy; D005455:Fluorescent Antibody Technique; D005753:Gastric Mucosa / Q000276:immunology; D006736:Horses; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000276:immunology*; D008722:Methods; D051379:Mice; D008832:Microchemistry; D008928:Mitochondria / Q000276:immunology; D009130:Muscle, Smooth / Q000276:immunology; D051381:Rats
D000906:Antibodies; D000941:Antigens