title: Specific inactivation of 16S ribosomal RNA induced by colicin E3 in vivo.
abstract: Treatment of sensitive Escherichia coli cells with colicin E3 leads to inactivation of 30S ribosomal subunits. In vitro reconstitution of 30S subunits indicates that the E3-induced defect lies solely in the 16S RNA. 16S RNA from E3-treated cells lacks several T(1) RNase oligonucleotides of normal 16S RNA, including the one from the 3'-end of the 16S RNA, as analyzed by the fingerprint technique of Sanger. An RNA fragment about 50 nucleotides long has been isolated from E3-treated cells. This RNA contains the original 3'-terminal oligonucleotide and other oligonucleotides missing in the E3-16S RNA. The results show that colicin E3 treatment causes the cleavage of 16S RNA at a specific position near the 3'-terminus.
mesh_terms: D000469:Alkaline Phosphatase; D003087:Colicins / Q000494:pharmacology*; D004589:Electrophoresis, Disc; D004926:Escherichia coli / Q000166:cytology / Q000378:metabolism*; D009711:Nucleotides / Q000032:analysis; D010179:Pancreas / Q000201:enzymology; D012329:RNA, Bacterial / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism; D012260:Ribonucleases; D012270:Ribosomes / Q000032:analysis / Q000187:drug effects*
chemical_list: D003087:Colicins; D009711:Nucleotides; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012260:Ribonucleases; D000469:Alkaline Phosphatase