Sequential translation of trinucleotide codons for peptide bond formation, translocation, and termination.
The addition of GUA, valyl-tRNA, T factor, GTP, and ethanol to reactions containing fMet-tRNA.AUG.ribosome intermediate results in the synthesis of fMet-valyl-tRNA which can be translocated if G factor is also present. The translocated peptidyl-tRNA can interact with puromycin to form fMet-Val-puromycin. Release of fMet-valine can also take place after translocation if a terminator trinucleotide and its specific release factor are present.
D002247:Carbon Isotopes; D004586:Electrophoresis; D004926:Escherichia coli / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D011691:Puromycin / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
D002247:Carbon Isotopes; D010455:Peptides; D012333:RNA, Messenger; D011691:Puromycin; D012343:RNA, Transfer