Replication of bacteriophage phiX174 in a temperature-sensitive deoxyribonucleic acid host cell.
Bacteriophage phiX174 is unable to replicate in Escherichia coli t3 at the restrictive temperature. However, if progeny phage synthesis is initiated at the permissive temperature, it will continue after a shift to the restrictive temperature.
D003090:Coliphages / Q000254:growth & development* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D005827:Genetics, Microbial; D009154:Mutation; D013696:Temperature; D013941:Thymine / Q000378:metabolism; D014316:Tritium; D014779:Virus Replication
D004269:DNA, Bacterial; D014316:Tritium; D013941:Thymine