title: Mutations affecting iron transport in Escherichia coli.
abstract: A mutant of Escherichia coli K-12 unable to form an essential component of the enterochelin-dependent iron transport system has been isolated. This strain carries a mutation in a gene designated fep, mapping close to two genes, entA and entD, concerned with enterochelin synthesis. Strain AN102, which carries the fep(-) allele, accumulates large quantities of enterochelin and gives a growth response to sodium citrate. The cytochrome b(1) and total iron content, and the measurement of the uptake of (55)Fe(3+), indicate an impairment of the enterochelin-dependent iron transport system. The growth response to sodium citrate is related to the presence, in strain AN102, of an inducible citrate-dependent iron transport system.
mesh_terms: D001692:Biological Transport; D002874:Chromosome Mapping; D002951:Citrates / Q000378:metabolism; D003580:Cytochromes / Q000378:metabolism; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D005796:Genes; D005827:Genetics, Microbial; D007501:Iron / Q000378:metabolism*; D007503:Iron Isotopes; D009154:Mutation; D014161:Transduction, Genetic
chemical_list: D002951:Citrates; D003580:Cytochromes; D007503:Iron Isotopes; D007501:Iron