Effects of essential amino acid starvation in Streptococcus faecalis: structural change in the 50S ribosomal subunit.
The 50S ribosomal subunits obtained from exponential-phase and valine-limited cultures of Streptococcus faecalis 9790 differed in protein content. Approximately 35% of the valine-limited subunits showed protein losses when analyzed by CsCl density centrifugation after formaldehyde fixation. Results of density gradient analysis and of acrylamide gel electrophoresis localized the protein to a "split" subunit fraction. No differences could be detected in the protein contents of 30S subunits from exponential-phase and valine-limited cultures.
D000179:Acrylates; D001120:Arginine / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis / Q000096:biosynthesis; D001431:Bacteriological Techniques; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D003470:Culture Media
D000179:Acrylates; D001426:Bacterial Proteins; D002247:Carbon Isotopes; D002712:Chlorides; D003470:Culture Media; D002586:Cesium; D001120:Arginine