title: Antigens of Neisseria gonorrhoeae: characterization by gel filtration, complement fixation, and agar-gel diffusion of antigens of gonococcal protoplasm.
abstract: With the use of the agar-gel-diffusion and complement-fixation techniques, it was shown that protoplasm from different gonococcal isolates reacted with sera from some humans with a history of gonorrhea but did not react with "normal" human sera. The reactive antigen(s) could be partially separated from the other antigens by passing the gonococcal protoplasm through Sephadex G-200. The antigen(s) reacting in the gel-diffusion and complement-fixation tests appeared in the same fraction. On the basis of Sephadex gel filtration, the molecular weight of this antigen(s) is probably greater than 200,000.
mesh_terms: D000818:Animals; D000941:Antigens / Q000032:analysis*; D002850:Chromatography, Gel; D003168:Complement Fixation Tests; D003593:Cytoplasm; D006069:Gonorrhea / Q000175:diagnosis / Q000276:immunology; D006801:Humans; D007106:Immune Sera; D005779:Immunodiffusion; D008970:Molecular Weight; D009344:Neisseria gonorrhoeae / Q000276:immunology*; D011817:Rabbits
chemical_list: D000941:Antigens; D007106:Immune Sera