[Cross-link in fibrin polymerized by factor 13: epsilon-(gamma-glutamyl)lysine].
(epsilon)-((gamma)-Glutamyl)lysine has been isolated from enzymatic hydrolyzates of cross-linked human fibrin. This compound was not detected in "non-cross-linked" fibrin prepared with ethylenediaminetetraacetic acid, which inhibits factor XIII; intermediate amounts were observed when the fibrin was prepared with glycine ethyl ester, which inhibits factor XIII competitively. These and ancillary experiments furnish conclusive evidence that epsilon-(gamma-glutamyl)lysine cross-links form in human fibrin during polymerization catalyzed by factor XIII.
D001322:Autoanalysis; D001639:Bicarbonates; D001777:Blood Coagulation; D001779:Blood Coagulation Factors; D002852:Chromatography, Ion Exchange; D003956:Dialysis; D004151:Dipeptides / Q000032:analysis*; D004492:Edetic Acid; D005337:Fibrin / Q000032:analysis*; D006801:Humans; D008239:Lysine / Q000032:analysis; D011108:Polymers / Q000032:analysis*
D001639:Bicarbonates; D001779:Blood Coagulation Factors; D004151:Dipeptides; D011108:Polymers; D005337:Fibrin; D004492:Edetic Acid; D008239:Lysine