Electrophoretic characteristics of staphylococcal hyaluronate lyase.
Partially purified staphylococcal hyaluronidase was studied with respect to its electrophoretic properties by the use of starch slurry or semisolid Noble agar as a matrix. The polarity of enzyme activity was found to be cathodal on starch and anodal on agar gel. The electrophoretic migration of the partially purified enzyme on starch, as a function of pH, suggested that the enzyme has an isoelectric point of pH 9.5 to 10.
D004586:Electrophoresis; D006821:Hyaluronoglucosaminidase / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D005779:Immunodiffusion; D013210:Staphylococcus / Q000201:enzymology*
D006821:Hyaluronoglucosaminidase