title: Polynucleotide homologies of Brucella deoxyribonucleic acids.
abstract: Deoxyribonucleic acids (DNA's) extracted from organisms presently placed in the genus Brucella (B. abortus, B. melitensis, B. neotomae, and B. suis) possessed very similar polynucleotide sequences. Unlabeled, single-stranded DNA fragments from B. abortus, B. melitensis, B. neotomae, and B. suis were equally effective in competing with the interaction of corresponding radiolabeled, single-stranded DNA fragments with their homologous DNA-agars. Unlabeled fragments of B. ovis, however, did not compete as effectively as the homologous, unlabeled DNA's, and this organism, therefore, had a detectably different polynucleotide composition. The mole percentages of guanine plus cytosine in Brucella DNA's (56 to 58%) were also similar. DNA's from Francisella tularensis, Escherichia coli, and the slow loris did not compete.
mesh_terms: D002002:Brucella / Q000032:analysis* / Q000145:classification; D002003:Brucella abortus / Q000032:analysis*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003596:Cytosine / Q000032:analysis; D004269:DNA, Bacterial / Q000032:analysis*; D004926:Escherichia coli / Q000032:analysis; D005604:Francisella tularensis / Q000032:analysis; D006147:Guanine / Q000032:analysis; D009691:Nucleic Acid Denaturation; D010759:Phosphorus Isotopes; D011119:Polynucleotides / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D010759:Phosphorus Isotopes; D011119:Polynucleotides; D006147:Guanine; D003596:Cytosine