Identification of the toxic component of Brucella abortus endotoxin and its labeling with radioactive chromate.
The evidence obtained from immuno-gel diffusion, centrifugation, and toxicity studies employing the serum iron assay proved that most of the toxicity in an ether-water brucella endotoxin preparation lies in the slow-diffusing component identified as the biologically active endotoxin. Subsequent destruction of the slow-diffusing component by acid hydrolysis resulted in a corresponding loss of toxicity. Chromate-51 was found to attach almost entirely on the slow-diffusing biologically active component and, hence, is a valid label for endotoxin derived from smooth Brucella abortus by the ether-water method.
D000818:Animals; D002003:Brucella abortus / Q000032:analysis*; D002840:Chromates / Q000378:metabolism*; D002859:Chromium Isotopes; D004731:Endotoxins / Q000032:analysis* / Q000633:toxicity; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007501:Iron / Q000097:blood; D051379:Mice; D011817:Rabbits; D014357:Trypsin / Q000494:pharmacology; D014461:Ultracentrifugation
D002840:Chromates; D002859:Chromium Isotopes; D004731:Endotoxins; D007501:Iron; D014357:Trypsin