title: Reductive pentose phosphate cycle in Nitrosocystis oceanus.
abstract: Campbell, Ann E. (Woods Hole Oceanographic Institution, Woods Hole, Mass.), Johan A. Hellebust, and Stanley W. Watson. Reductive pentose phosphate cycle in Nitrosocystis oceanus. J. Bacteriol. 91:1178-1185. 1966.-Assays in cell-free extracts of Nitrosocystis oceanus, a marine chemoautotrophic bacterium, have demonstrated the presence of all of the enzymes of the reductive pentose phosphate cycle, with activities high enough to account for the normal growth rate of the cells. Studies on ribulosediphosphate carboxylase activity in these extracts showed that it is inhibited by MgCl(2) (30% at 0.01 m), MnCl(2) (70% at 0.01 m), NaCl and KCl (100% at 0.5 m, 63% at 0.2 m), and by sulfate (35% at 0.01 m); phosphate, glutathione, and ethylenediaminetetraacetic acid had no effect. The bacterial enzyme differs from the spinach enzyme with respect to its affinity for bicarbonate and its pH optimum. Whole cells were incubated with C(14)O(2), and the acid-soluble fraction was analyzed by paper chromatography and autoradiography. Phosphoglyceric acid and the sugar phosphates were the earliest labeled compounds; several amino acids and organic acids were also labeled. It is concluded that N. oceanus incorporates CO(2) primarily via the reductive pentose phosphate cycle.
mesh_terms: D001419:Bacteria / Q000201:enzymology* / Q000378:metabolism*; D002247:Carbon Isotopes / Q000378:metabolism; D002262:Carboxy-Lyases / Q000378:metabolism; D002712:Chlorides / Q000494:pharmacology; D002854:Chromatography, Paper; D004492:Edetic Acid / Q000494:pharmacology; D004798:Enzymes; D005978:Glutathione / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D009586:Nitrogen Fixation; D010428:Pentosephosphates / Q000378:metabolism*; D010710:Phosphates / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013431:Sulfates / Q000494:pharmacology
chemical_list: D002247:Carbon Isotopes; D002712:Chlorides; D004798:Enzymes; D010428:Pentosephosphates; D010710:Phosphates; D013431:Sulfates; D008345:Manganese; D012965:Sodium Chloride; D011189:Potassium Chloride; D004492:Edetic Acid; D002262:Carboxy-Lyases; D005978:Glutathione; D008274:Magnesium