Inactivation of kanamycin, neomycin, and streptomycin by enzymes obtained in cells of Pseudomonas aeruginoa.
Ten strains of Pseudomonas aeruginosa were disrupted and centrifuged. The supernatant fluids from centrifugation at 105,000 x g contained enzymes inactivating kanamycin, neomycin, and streptomycin in the presence of adenosine triphosphate. Kanamycin-inactivating enzyme was precipitated with ammonium sulfate at 66% of saturated concentration, and the inactivated kanamycin was shown to be kanamycin-3'-phosphate in which the C-3 hydroxyl group of 6-amino-6-deoxy-d-glucose moiety was phosphorylated. This is identical with kanamycin inactivated by Escherichia coli carrying R factor. Streptomycin-inactivating enzyme was precipitated with ammonium sulfate at 33% of saturated concentration.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D002474:Cell-Free System; D002850:Chromatography, Gel; D004352:Drug Resistance, Microbial; D004798:Enzymes / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007612:Kanamycin / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D009355:Neomycin / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000378:metabolism; D013307:Streptomycin / Q000378:metabolism*
D004798:Enzymes; D007612:Kanamycin; D000255:Adenosine Triphosphate; D009355:Neomycin; D008274:Magnesium; D013307:Streptomycin