Microcycle sporogenesis of Bacillus cereus in a chemically defined medium.
A chemically defined medium which allowed germination, outgrowth, and subsequent resporulation of Bacillus cereus T spores, without intervening cell division (microcycle sporogenesis), is described. No medium replacement was required. The second-stage spores were heat-stable and had similar germination characteristics and dipicolinic acid content to primary spores. Deoxyribonucleic acid (DNA) replication began soon after germination and there was a doubling in the DNA content of the cells within 2 hr.
D001409:Bacillus cereus / Q000187:drug effects / Q000254:growth & development*; D003470:Culture Media; D004269:DNA, Bacterial / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D008937:Mitomycins / Q000494:pharmacology; D010710:Phosphates / Q000494:pharmacology; D010848:Picolinic Acids / Q000032:analysis; D013170:Spores / Q000254:growth & development; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014498:Uracil / Q000378:metabolism
D003470:Culture Media; D004269:DNA, Bacterial; D008937:Mitomycins; D010710:Phosphates; D010848:Picolinic Acids; D014316:Tritium; D003609:Dactinomycin; D014498:Uracil; D013936:Thymidine