Interactions between the lysine-rich histone F1 and deoxyribonucleic acid.
1. The interactions of the lysine-rich histone F1 with DNA have been studied at various histone to DNA ratios, in water and in the presence of uni- and bi-valent cations. In water only, histone F1, even in fourfold excess, is unable to precipitate all the DNA. In 0.14m-sodium chloride, 0.8mg. of histone F1 is required to precipitate 1mg. of DNA, whereas in 0.07m-magnesium chloride only 0.4mg. is required. 2. Bivalent cations are also shown to be more effective in dissociating the DNA-histone complex. Histone F1 can be selectively removed from deoxyribonucleoprotein with 0.1m-magnesium chloride. 3. The precipitation of DNA by histone F1 is a reversible process and the complex can be taken in and out of solution by changing the ionic environment. 4. The bearing of these results on the observed ability of various DNA-histone complexes to act as templates for RNA synthesis is discussed.
D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002621:Chemistry; D004247:DNA; D004492:Edetic Acid; D006657:Histones; D008239:Lysine; D008274:Magnesium; D012313:RNA / Q000096:biosynthesis; D012965:Sodium Chloride; D012996:Solutions
D006657:Histones; D012996:Solutions; D012965:Sodium Chloride; D012313:RNA; D004247:DNA; D004492:Edetic Acid; D008274:Magnesium; D008239:Lysine