title: Deoxyribonucleic acid degradation in Bacillus subtilis during exposure to actinomycin D.
abstract: At high concentrations (10 mug/ml), actinomycin D inhibited deoxyribonucleic acid (DNA) synthesis in Bacillus subtilis. Inhibition occurred quickly (in less than 1 min) and was complete. In strain 23 thy his, inhibition of DNA synthesis by actinomycin D was followed by partial degradation of one of the two daughter strands to acid-soluble products. Degradation began at the replication point and proceeded over a distance equal to about 12% of a chromosome in length. Actinomycin D played some essential part in degradation, since exposure of the cells to other treatments or agents which inhibit growth did not lead to the above result.
mesh_terms: D000166:Acridines / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects* / Q000378:metabolism; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D002876:Chromosomes, Bacterial / Q000187:drug effects*; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000378:metabolism*; D003609:Dactinomycin / Q000494:pharmacology*; D008962:Models, Theoretical; D009584:Nitrogen / Q000494:pharmacology; D012329:RNA, Bacterial / Q000096:biosynthesis; D011874:Radiometry; D013941:Thymine / Q000378:metabolism; D014316:Tritium
chemical_list: D000166:Acridines; D000900:Anti-Bacterial Agents; D002247:Carbon Isotopes; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D014316:Tritium; D003609:Dactinomycin; D009584:Nitrogen; D013941:Thymine