title: Catechol oxygenase induction in Pseudomonas aeruginosa.
abstract: 1. The transfer from benzenesulphonate to benzoate as a growth substrate for Pseudomonas aeruginosa strain A resulted in a change in the enzymic route by which catechol was degraded; at intermediate stages it was possible to obtain cells containing the enzymes of both the ;ortho' and ;meta' metabolic pathways. 2. A similar result was effected by the reverse transfer, benzoate to benzenesulphonate. 3. Catechol itself always elicited a catechol 2,3-oxygenase in uninduced cells, but the product of this reaction, 2-hydroxymuconic semialdehyde, and biochemically related compounds such as 4-hydroxy-2-oxovalerate, unexpectedly induced a catechol 1,2-oxygenase. 4. Both types of catechol oxygenase are strongly repressed by the metabolic end products of both the ;ortho' and ;meta' pathways, but there was no inhibition of enzymic activity by these end products.
mesh_terms: D001565:Benzoates / Q000378:metabolism; D002396:Catechols / Q000378:metabolism*; D004790:Enzyme Induction; D004794:Enzyme Repression; D005650:Fumarates / Q000378:metabolism; D006128:Growth; D010105:Oxygenases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D013451:Sulfonic Acids / Q000378:metabolism; D013997:Time Factors
chemical_list: D001565:Benzoates; D002396:Catechols; D005650:Fumarates; D013451:Sulfonic Acids; D010105:Oxygenases