The presence of glycopeptide material in cultured mouse mast-cell tumours.
1. Mouse mast-cell tumours P815 Y and HC were shown to contain glycoprotein material composed of glucosamine, galactosamine, sialic acid, galactose and mannose. 2. The major amino acids released after acid hydrolysis of Pronase-treated digests of the glycoprotein are aspartic acid, glutamic acid, serine, threonine, proline, glycine and alanine. The Pronase-digested material is not degraded in alkaline solution. 3. On incubation of mast cells with [(35)S]sulphate, heparin is the major radioactive product. However, [1-(14)C]glucosamine and d-[(14)C]glucose are incorporated largely into the glycoprotein. 4. The fate of [(35)S]sulphate-labelled and [1-(14)C]glucosamine-labelled material was studied. In each case high-molecular-weight radioactive material is released from the cells into the culture medium. The t((1/2)) of [(35)S]sulphate-labelled material in cells is 70hr. and that of [1-(14)C]-glucosamine-labelled material in cells is 40hr. 5. About 60% of the [(35)S]sulphate-labelled material is present in the mitochondrial and granular fraction. [1-(14)C]-Glucosamine-labelled material is present in both microsomal and mitochondrial and granular fractions, [(14)C]sialic acid being concentrated in the microsomal fraction.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002247:Carbon Isotopes; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D046508:Culture Techniques; D005690:Galactose / Q000032:analysis; D005944:Glucosamine / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis / Q000378:metabolism*; D006595:Hexosamines / Q000032:analysis; D008358:Mannose / Q000032:analysis; D012515:Mast-Cell Sarcoma / Q000378:metabolism*; D051379:Mice; D009363:Neoplasm Proteins / Q000032:analysis / Q000378:metabolism*; D009438:Neuraminic Acids / Q000032:analysis; D010447:Peptide Hydrolases; D010455:Peptides / Q000378:metabolism*; D012513:Sarcoma, Experimental / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism; D013460:Sulfur Isotopes
D000596:Amino Acids; D002247:Carbon Isotopes; D006023:Glycoproteins; D006595:Hexosamines; D009363:Neoplasm Proteins; D009438:Neuraminic Acids; D010455:Peptides; D013431:Sulfates; D013460:Sulfur Isotopes; D010447:Peptide Hydrolases; D005944:Glucosamine; D008358:Mannose; D005690:Galactose