Fractionation of transformable bacteria from ocompetent cultures of Bacillus subtilis on renografin gradients.
A population of Bacillus subtilis can be fractionated into at least two components on the basis of their buoyant densities on a Renografin gradient. In competent cultures, most, and perhaps all, of the competent bacteria appear in the lighter fraction which composes 2 to 10% of the bulk population. These "lighter" bacteria are the only bacteria capable of incorporating transforming deoxyribonucleic acid.
D001412:Bacillus subtilis; D002499:Centrifugation, Density Gradient; D004269:DNA, Bacterial / Q000378:metabolism; D003973:Diatrizoate; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D003973:Diatrizoate