Hapten carrier relationships in the DNP-PLL foreign albumin complex system: induction of tolerance and stimulation of cells in vitro.
Genetic nonresponder guinea pigs made tolerant to BSA and then immunized with DNP-PLL.BSA failed to make anti-DNP-PLL antibodies. Thus, tolerance to a carrier protein renders animals unresponsive to the hapten which it bears. The addition in vitro of DNP-PLL or DNP-GL to lymph node cell cultures derived from genetic responder animals immunized with these materials led to a significant stimulation of (3)H-thymidine incorporation into DNA. However, the addition of DNP-PLL or DNP-GL to lymph node cell cultures from nonresponder animals immunized with these materials failed to produce any stimulation of DNA synthesis. Furthermore, the addition of DNP-PLL to lymph node cell cultures from nonresponder animals immunized with DNP-PLL.BSA or DNP-PLL.OVA also failed to stimulate cell proliferation in spite of the fact that the lymph node cells of these animals were producing anti-DNP-PLL antibodies. The above facts suggest that the function of the PLL gene product is to act at an early crucial step in the immune mechanism to form an antigen-inducer complex. The specificity of this early step may be of a simple order and different than that of the antibody which is later produced in the immune response.
D000818:Animals; D000917:Antibody Formation; D000941:Antigens; D004247:DNA / Q000096:biosynthesis; D005823:Genetics; D005971:Glutamates; D006168:Guinea Pigs; D006241:Haptens; D006386:Hemagglutination Tests; D006968:Hypersensitivity, Delayed; D007108:Immune Tolerance; D005779:Immunodiffusion; D066298:In Vitro Techniques; D008198:Lymph Nodes / Q000276:immunology; D008239:Lysine; D010047:Ovalbumin; D012710:Serum Albumin, Bovine; D013936:Thymidine; D014316:Tritium
D000941:Antigens; D005971:Glutamates; D006241:Haptens; D014316:Tritium; D012710:Serum Albumin, Bovine; D010047:Ovalbumin; D004247:DNA; D008239:Lysine; D013936:Thymidine