title: Induction and propagation of a Bacillus subtilis L form in natural and synthetic media.
abstract: The L form of Bacillus subtilis NRRL B-3275 was induced in a 7% NaCl broth medium and subsequently propagated in natural and synthetic media. The L form grew readily in tryptone broth supplemented with glucose, NaCl, and phosphate buffer, and in a synthetic medium containing only glucose and biotin, in addition to the required salts. Successive transfers from the bacillus inoculum and subsequent large bodies in the tryptone broth with 7% NaCl resulted in gradual selection or transition from the bacillary form to a stable L form without the addition of an antibiotic. The number of viable granules attained in the broth culture exceeded 9 x 10(7) per ml, and numerous large bodies were always present in rapidly growing cultures.
mesh_terms: D001412:Bacillus subtilis / Q000187:drug effects / Q000254:growth & development; D003470:Culture Media; D007740:L Forms / Q000254:growth & development; D008858:Microscopy, Phase-Contrast; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D003470:Culture Media; D012965:Sodium Chloride