Localization of parental deoxyribonucleic acid from superinfecting T4 bacteriophage in Escherichia coli.
High-resolution autoradiography has been employed to localize the nonsolubilized but genetically excluded deoxyribonucleic acid (DNA) of T4 bacteriophage superinfecting endonuclease I-deficient Escherichia coli. This DNA was found to be associated with the cell envelope (this term is used here to include all cellular components peripheral to and including the cytoplasmic membrane); in contrast, T4 DNA in primary infected cells, like host DNA in uninfected E. coli, was found to be near the cell center. The envelope-associated DNA from super-infecting phage was not located on the outermost surface of the cell since it was insensitive to deoxyribonuclease added to the medium. These results suggest that DNA from superinfecting T-even phage is trapped within the cell envelope.
D001345:Autoradiography; D002473:Cell Wall / Q000378:metabolism; D002701:Chloramphenicol / Q000494:pharmacology; D003090:Coliphages / Q000032:analysis / Q000254:growth & development* / Q000378:metabolism; D004279:DNA, Viral / Q000302:isolation & purification* / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D004926:Escherichia coli / Q000032:analysis* / Q000201:enzymology / Q000378:metabolism; D008854:Microscopy, Electron; D009154:Mutation; D013104:Spheroplasts; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014762:Viral Interference
D004279:DNA, Viral; D014316:Tritium; D002701:Chloramphenicol; D003851:Deoxyribonucleases; D013936:Thymidine