Kinetics and immunoglobulin nature of the antibody response to Mycobacterium tuberculosis.
Antibody production after injection of Mycobacterium tuberculosis was studied by use of the single cell plaque assay technique. Maximal numbers of antibody-forming cells were found 9 days after a single injection of M. tuberculosis, about 1 day before the appearance of maximal circulating antibody. Immunoelectrophoretic studies and 2-ME studies on 19S and 7S globulin fractions obtained by gel filtration show that the hemagglutination and hemagglutination-lysis antibody activity is associated with 19S immunoglobulin.
D000818:Animals; D000917:Antibody Formation; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D007700:Kinetics; D008297:Male; D008623:Mercaptoethanol / Q000494:pharmacology; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011817:Rabbits
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008623:Mercaptoethanol