Development of competence in thymine-starved Bacillus subtilis with chromosomes arrested at the terminus.
Tryptophan- and thymine-requiring cells of Bacillus subtilis, emerging from an amino acid starvation treatment which causes arrest of the chromosomes at the terminus, were not transformable. During subsequent incubation in a thymineless medium supplemented with amino acids, the cultures developed competence while retaining chromosome arrest. The competent subpopulation apparently shares the synchronous chromosome arrest of the bulk population. This was shown by different methods. The principal method was marker frequency analysis of the deoxyribonucleic acid extracted from a population enriched for competent cells by a column-chromatographic method. It is concluded that development of the competent state can occur in nondividing cells, and that the presence of a replication fork actively engaged in synthesis of deoxyribonucleic acid is not required for the development of this state.
D001412:Bacillus subtilis; D002455:Cell Division; D002852:Chromatography, Ion Exchange; D002876:Chromosomes, Bacterial; D003470:Culture Media; D004261:DNA Replication; D005827:Genetics, Microbial; D009154:Mutation; D013941:Thymine; D013997:Time Factors; D014364:Tryptophan
D003470:Culture Media; D014364:Tryptophan; D013941:Thymine