title: Quantitative aspects of exchangeable calcium in spores of Bacillus megaterium.
abstract: Rode, L. J. (The University of Texas, Austin), and J. W. Foster. Quantitative aspects of exchangeable calcium in spores of Bacillus megaterium. J. Bacteriol. 91:1589-1593. 1966.-More than 90% of the calcium in Ca(45)-labeled native spores was released from the cells during germination. Some 95% of the spore calcium was not exchangeable when ungerminated native spores were titrated to pH 4 with HNO(3). Ca, Mg, Na, Si, and Fe were displaced from the spores by H(+). The adsorption of Ca(45) by H-spores and its subsequent release were studied under a variety of conditions. The isolated "coat fraction" of spores adsorbed substantial amounts of Ca(45). Release of the adsorbed Ca was achieved with various reagents.
mesh_terms: D000327:Adsorption; D001410:Bacillus megaterium / Q000254:growth & development*; D002118:Calcium / Q000378:metabolism*; D002127:Calcium Isotopes; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007501:Iron; D008274:Magnesium; D012825:Silicon; D012964:Sodium; D013170:Spores; D013696:Temperature
chemical_list: D002127:Calcium Isotopes; D012964:Sodium; D007501:Iron; D008274:Magnesium; D002118:Calcium; D012825:Silicon