title: Tracer studies to locate the site of platinum ions within filamentous and inhibited cells of Escherichia coli.
abstract: The distribution of platinum ions within Escherichia coli after the induction of filaments with cis-Pt(NH(3))(2)Cl(4), and after growth inhibition by PtCl(6) (2-), has been determined with radioactive metal compounds ((191)Pt, with a half-life of approximately 3 days) by the simple chemical procedure of Roberts et al. In the filamentous cells, the platinum metal is associated with metabolic intermediates, nucleic acids, and cytoplasmic proteins; whereas, in inhibited cells, the platinum is combined only with the cytoplasmic protein. Similar experiments with gram-positive cells of Bacillus cereus and Staphyloccus aureus, which show no filamentous growth in the presence of cis-Pt(NH(3))(2)Cl(4), reveal that the metal complex does penetrate the cell wall and subsequently becomes bound predominantly by metabolic intermediates.
mesh_terms: D001409:Bacillus cereus / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004926:Escherichia coli / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D010984:Platinum / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology; D011868:Radioisotopes; D013210:Staphylococcus / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D011868:Radioisotopes; D010984:Platinum