title: The cell walls of Pseudomonas aeruginosa. General composition.
abstract: 1. Cell walls of Pseudomonas aeruginosa were prepared and analysed. 2. Separate preparations were found to be reproducible, e.g. the phosphorus contents of all batches lay in the range 2.0-2.1%. 3. Ninhydrin-positive compounds in hydrolysates accounted for 43-46% of the cell wall and 79-87% of the nitrogen of the cell wall. Examination of the results for individual ninhydrin-positive compounds showed that 5-15% of the cell wall was murein and about 30% protein. 4. Trypsin treatment of crude cell-wall preparations preferentially liberated arginine, lysine and leucine, but it was not clear whether these arose from cell-wall or cytoplasmic proteins.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000641:Ammonia / Q000032:analysis; D001322:Autoanalysis; D001426:Bacterial Proteins / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D002473:Cell Wall / Q000032:analysis*; D002854:Chromatography, Paper; D005227:Fatty Acids / Q000032:analysis; D006595:Hexosamines / Q000032:analysis; D007202:Indicators and Reagents; D009584:Nitrogen / Q000032:analysis; D010758:Phosphorus / Q000032:analysis; D011550:Pseudomonas aeruginosa / Q000032:analysis*; D013053:Spectrophotometry
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D002241:Carbohydrates; D005227:Fatty Acids; D006595:Hexosamines; D007202:Indicators and Reagents; D010758:Phosphorus; D000641:Ammonia; D009584:Nitrogen