title: Fate of thymine-containing dimers in the deoxyribonucleic acid of ultravioletirradiated Bacillus subtilis.
abstract: The fate of ultraviolet-induced, thymine-containing dimers in the deoxyribonucleic acid (DNA) of Bacillus subtilis was investigated in both the wild type (UV(R)) and an ultraviolet light-sensitive (UV(S)) mutant. During incubation in the dark, dimers were excised from the DNA of the UV(R)B. subtilis, but remained in the DNA of the UV(S) mutant. About 40% of the excised dimers recovered in the wild type were in the acid-soluble fraction; the remainder were in the incubation medium. A UV(S) mutant of Escherichia coli K-12, shown previously to be defective in dimer excision, was irradiated with ultraviolet light and incubated under visible light for 3 hr. About 65% of thymine-containing photoproducts were removed from the DNA. These photoproducts were not recovered in the acid-soluble fraction. In comparison, the UV(S) mutant of B. subtilis lost only 13% of such photoproducts from DNA when exposed to light under the same conditions.
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism* / Q000528:radiation effects*; D004269:DNA, Bacterial / Q000378:metabolism*; D008027:Light; D011830:Radiation Effects; D013941:Thymine / Q000378:metabolism; D014316:Tritium; D014466:Ultraviolet Rays
chemical_list: D004269:DNA, Bacterial; D014316:Tritium; D013941:Thymine