Improved assay method for phospholipase C.
A lecithin sol dispersed with deoxycholate was found to be attacked by phospholipase C in the presence of calcium ion more rapidly than were any other lecithin sols. The inorganic phosphate could be released quantitatively from the acid soluble phosphate liberated from lecithin by an excess amount of alkaline phosphatase present in phospholipase C reaction mixture. A simple and accurate assay method for phospholipase C was developed with the sol and the alkaline phosphatase.
D000469:Alkaline Phosphatase / Q000378:metabolism; D001409:Bacillus cereus / Q000201:enzymology; D003013:Clostridium / Q000201:enzymology; D003902:Detergents / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology; D010710:Phosphates / Q000032:analysis; D010713:Phosphatidylcholines / Q000378:metabolism; D010740:Phospholipases / Q000032:analysis*; D011550:Pseudomonas aeruginosa / Q000201:enzymology
D003902:Detergents; D010710:Phosphates; D010713:Phosphatidylcholines; D010740:Phospholipases; D000469:Alkaline Phosphatase