Preparation of 14C-labeled tuberculin purified protein derivative.
(14)C-labeled tuberculin purified protein derivative ((14)C-tuberculin PPD) has been prepared from culture filtrates of Mycobacterium tuberculosis var. hominis grown in a culture medium containing uniformly labeled (14)C-amino acids. With a mixture of (14)C-amino acids (an acid hydrolysate of (14)C-Chlorella protein) in the medium, the recoveries of (14)C in the final product were higher than with (14)C-labeled-l-glutamic acid. (14)C-tuberculin PPD was separated into tuberculoprotein and nucleic acid by paper electrophoresis. The specific radioactivity of tuberculoprotein was substantially greater than that of the nucleic acid. (14)C-tuberculin PPD is advocated as a means to measure the adsorption of tuberculin to glass or other surfaces. It could also prove useful as a means to study the structure and mode of action of tuberculin.
D002247:Carbon Isotopes; D004586:Electrophoresis; D005971:Glutamates / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D014373:Tuberculin / Q000032:analysis / Q000096:biosynthesis*
D002247:Carbon Isotopes; D005971:Glutamates; D011506:Proteins; D014373:Tuberculin