Antibodies to deoxyribonucleic acid irradiated with ultraviolet light: detection by precipitins and immunofluorescence.
Two rabbits were immunized with a complex consisting of ultravioietirradiated DNA and methylated bovine serum albumin. Serum antibodies reacted only with irradiated DNA, and the serological reaction was shown by immuneprecipitation and immunofluorescence.
D000818:Animals; D000906:Antibodies; D011232:Chemical Precipitation; D004247:DNA / Q000528:radiation effects*; D005455:Fluorescent Antibody Technique; D006358:Hot Temperature; D007106:Immune Sera; D005779:Immunodiffusion; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D008745:Methylation; D009691:Nucleic Acid Denaturation; D011233:Precipitin Tests; D011817:Rabbits; D011830:Radiation Effects; D011854:Radiochemistry; D012710:Serum Albumin, Bovine; D013950:Thymus Gland; D014466:Ultraviolet Rays
D000906:Antibodies; D007106:Immune Sera; D012710:Serum Albumin, Bovine; D004247:DNA