Determination of amino sugars in mixtures containing glucosamine, galactosamine and muramic acid.
A colorimetric method is described whereby the direct quantitative determination of glucosamine, galactosamine and muramic acid can be achieved without previous treatment of the cell-wall hydrolysate, for example by column chromatography. Molar ratios of hexosamines in cell-wall preparations, from a number of bacterial species, determined by this method were found to be in general agreement with previously published results.
D000606:Amino Sugars / Q000032:analysis*; D001419:Bacteria / Q000032:analysis*; D002473:Cell Wall / Q000032:analysis*; D002845:Chromatography; D003124:Colorimetry; D013293:Enterococcus faecalis / Q000166:cytology; D004926:Escherichia coli / Q000166:cytology; D005690:Galactose; D005944:Glucosamine / Q000032:analysis*; D006595:Hexosamines / Q000032:analysis*; D008837:Micrococcus / Q000166:cytology; D009161:Mycobacterium / Q000166:cytology; D011511:Proteus / Q000166:cytology; D011550:Pseudomonas aeruginosa / Q000166:cytology; D013210:Staphylococcus / Q000166:cytology
D000606:Amino Sugars; D006595:Hexosamines; D005944:Glucosamine; D005690:Galactose