Co-linearity of beta-galactosidase with its gene by immunological detection of incomplete polypeptide chains.
Many nonsense mutants that map in the beta-galactosidase structural gene produce material that forms precipitin lines when tested by double diffusion on agar against antiserum prepared from native beta-galactosidase. Relative sizes of the cross-reacting materia!s measured by sucrose density gradient centrifugation are the same as sizes calculated from genetic mapping of nonsense mutants. Orientation of the protein to its gene is also indicated.
D002874:Chromosome Mapping; D004926:Escherichia coli / Q000276:immunology*; D005696:Galactosidases; D005796:Genes; D005779:Immunodiffusion; D009154:Mutation; D014461:Ultracentrifugation
D005696:Galactosidases