In vitro attachment of radioactive endotoxins to lysosomes.
The experiments reported here demonstrate that under certain conditions endotoxin can interact with lysosomes in vitro. After incubation of large granular fraction with (51)Cr-labeled antigen under the conditions required for acid hydrolytic activity, radioactivity was associated with the pellet after centrifugation. This effect can be inhibited by preincubation of the large granular fraction with unlabeled homologous or heterologous endotoxins. High resolution autoradiography showed that (14)C-labeled endotoxin was predominantly attached to the lysosomes contained in the large granular fraction. The mechanism of this interaction and its relationship to the toxic effect of endotoxins on lysosomes are discussed.
D000818:Animals; D000942:Antigens, Bacterial / Q000302:isolation & purification; D001345:Autoradiography; D001426:Bacterial Proteins / Q000032:analysis; D002247:Carbon Isotopes; D002859:Chromium Isotopes; D004731:Endotoxins / Q000378:metabolism*; D004926:Escherichia coli / Q000032:analysis; D006596:Hexosaminidases / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology; D008247:Lysosomes / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D012475:Salmonella / Q000276:immunology; D012477:Salmonella enteritidis / Q000276:immunology; D013154:Spleen / Q000166:cytology; D013347:Subcellular Fractions; D013997:Time Factors
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D002247:Carbon Isotopes; D002859:Chromium Isotopes; D004731:Endotoxins; D006596:Hexosaminidases