title: Metabolic requirements for microcycle sporogenesis of Bacillus megaterium.
abstract: Spores of Bacillus megaterium QM B1551 germinated, elongated, and resporulated (microcycle sporogenesis) in simple chemically defined media which permitted no cell division. The second-stage spores thus produced were heat-stable and required heat activation for germination. The original amount of spore deoxyribonucleic acid tripled before completion of the cycle. Acetate and a small amount of a tricarboxylic acid cycle intermediate were the minimal organic metabolic requirements for microcycle sporogenesis. During this cycle, germinated cells oxidized acetate only after a delay, whether or not glucose was initially present. Spores that were germinated in the absence of a carbon source first oxidized an endogenous substrate, and then developed the ability to oxidize acetate.
mesh_terms: D000641:Ammonia / Q000378:metabolism; D001410:Bacillus megaterium / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D003470:Culture Media; D004269:DNA, Bacterial / Q000378:metabolism; D005463:Fluoroacetates / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D006358:Hot Temperature; D006836:Hydro-Lyases / Q000378:metabolism; D011725:Pyridines / Q000378:metabolism; D013170:Spores
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D005463:Fluoroacetates; D011725:Pyridines; D000641:Ammonia; D006836:Hydro-Lyases; D005947:Glucose