Polynucleotide homologies of Brucella deoxyribonucleic acids.
Deoxyribonucleic acids (DNA's) extracted from organisms presently placed in the genus Brucella (B. abortus, B. melitensis, B. neotomae, and B. suis) possessed very similar polynucleotide sequences. Unlabeled, single-stranded DNA fragments from B. abortus, B. melitensis, B. neotomae, and B. suis were equally effective in competing with the interaction of corresponding radiolabeled, single-stranded DNA fragments with their homologous DNA-agars. Unlabeled fragments of B. ovis, however, did not compete as effectively as the homologous, unlabeled DNA's, and this organism, therefore, had a detectably different polynucleotide composition. The mole percentages of guanine plus cytosine in Brucella DNA's (56 to 58%) were also similar. DNA's from Francisella tularensis, Escherichia coli, and the slow loris did not compete.
D002002:Brucella / Q000032:analysis* / Q000145:classification; D002003:Brucella abortus / Q000032:analysis*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003596:Cytosine / Q000032:analysis; D004269:DNA, Bacterial / Q000032:analysis*; D004926:Escherichia coli / Q000032:analysis; D005604:Francisella tularensis / Q000032:analysis; D006147:Guanine / Q000032:analysis; D009691:Nucleic Acid Denaturation; D010759:Phosphorus Isotopes; D011119:Polynucleotides / Q000032:analysis
D004269:DNA, Bacterial; D010759:Phosphorus Isotopes; D011119:Polynucleotides; D006147:Guanine; D003596:Cytosine