Hydrolysis of synthetic esters by the antibacterial agent in serum.
Yotis, William W. (Loyola University, Chicago, Ill.). Hydrolysis of synthetic esters by the antibacterial agent in serum. J. Bacteriol. 91:488-493. 1966.-An antistaphylococcal serum agent was assayed colorimetrically, manometrically, and titrimetrically for esterase activity. p-Nitrophenol acetate, triacetin, l-lysine methyl and ethyl ester, and norleucine methyl ester were hydrolyzed by the antistaphylococcal agent. Acetylcholine and benzoylcholine esters, triolein, tristearin, and p-tosylarginine methyl ester were not attacked by this agent. With p-nitrophenol acetate as substrate, optimal activity occurred at pH 7.4. Incubation at 60 C for 30 min reduced drastically the esterase activity of the antistaphylococcal agent, and incubation at 75 C for 30 min abolished the esterase activity of this agent. Almost complete inhibition of esterase activity was observed with 0.001 m HgCl(2), ZnSO(4), and ethylenediaminetetraacetic acid (EDTA). EDTA inhibition could be reversed by the addition of CaCl(2), but not MgCl(2). Cysteine reversed the inhibition of HgCl(2). NaF, atoxyl, diisopropyl fluorophosphate, quinine, and physostigmine did not influence the esterase activity of the antibacterial agent. The demonstration of esterase activity of both the antistaphylococcal agent and coagulase may shed further light on the reported ability of coagulase to neutralize the antistaphylococcal activity of this agent, or the prevention of absorption of the agent on the staphylococcal cell surface. In addition, the colorimetric procedure described in this report may be a convenient tool in assaying the potency of the antistaphylococcal agent.
D000085:Acetates; D000109:Acetylcholine; D001769:Blood; D001770:Blood Bactericidal Activity; D002122:Calcium Chloride / Q000494:pharmacology; D055598:Chemical Phenomena; D002621:Chemistry; D003545:Cysteine / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004950:Esterases; D005030:Ethylenes; D008239:Lysine; D008274:Magnesium / Q000494:pharmacology; D008628:Mercury / Q000494:pharmacology; D009596:Nitrophenols; D010830:Physostigmine / Q000494:pharmacology; D011803:Quinine / Q000494:pharmacology; D013210:Staphylococcus / Q000187:drug effects; D013229:Stearic Acids; D014215:Triacetin; D014304:Triolein; D015032:Zinc / Q000494:pharmacology
D000085:Acetates; D005030:Ethylenes; D009596:Nitrophenols; D013229:Stearic Acids; D014304:Triolein; D004492:Edetic Acid; D010830:Physostigmine; D011803:Quinine; D004950:Esterases; D008628:Mercury; D008274:Magnesium; D015032:Zinc; D008239:Lysine; D003545:Cysteine; D002122:Calcium Chloride; D000109:Acetylcholine; D014215:Triacetin