Isopycnic centrifugation for the isolation of DNA strands coding for ribosomal RNA.
Denatured DNA preparations from Escherichia coli were centrifuged to equilibrium in cesium chloride solutions. Hybridizing experiments with radioactively labeled ribosomal RNA showed that the DNA strands complementary to ribosomal RNA were distributed on the heavy side of the DNA band. By fractionating this band the DNA strands coding for ribosomal RNA may be enriched 5- to 20- fold.
D001410:Bacillus megaterium; D002498:Centrifugation; D002586:Cesium; D011232:Chemical Precipitation; D002623:Chemistry Techniques, Analytical; D004269:DNA, Bacterial; D004926:Escherichia coli; D066298:In Vitro Techniques; D010759:Phosphorus Isotopes; D012329:RNA, Bacterial; D011874:Radiometry; D012270:Ribosomes
D004269:DNA, Bacterial; D010759:Phosphorus Isotopes; D012329:RNA, Bacterial; D002586:Cesium