title: Extraction, characterization, and cellular localization of the lipids of Staphylococcus aureus.
abstract: Satisfactory extraction and assay procedures have been developed for the lipids of Staphylococcus aureus. The following lipids have been characterized in detail: the vitamin K(2), which is shown to exist as isoprenologues with side chains of 35, 40, and 45 carbon atoms; monoglucosyldiglyceride and diglucosyldiglyceride, which account for all the carbohydrate in the lipid extracts; the lysyl ester of phosphatidyl glycerol, phosphatidyl glycerol, and cardiolipin, which account for 98% of the phosphate in the lipid extract. The extraction procedure removes 98% of the total bacterial fatty acids. Acidification of the medium before harvest and refluxing in isopropanol are critical in the extraction procedure for the maximal recovery of lysyl-phosphatidyl glycerol and the glucolipids. The lipids have been shown to be a part of the same membrane as the respiratory pigments.
mesh_terms: D002256:Carbonic Anhydrases / Q000378:metabolism; D002462:Cell Membrane / Q000032:analysis; D002845:Chromatography; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D003580:Cytochromes / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006017:Glycolipids / Q000032:analysis; D008055:Lipids / Q000032:analysis*; D008722:Methods; D010101:Oxygen Consumption; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis; D011809:Quinones / Q000032:analysis; D013210:Staphylococcus / Q000032:analysis* / Q000378:metabolism; D014812:Vitamin K / Q000032:analysis
chemical_list: D003580:Cytochromes; D006017:Glycolipids; D008055:Lipids; D010743:Phospholipids; D011809:Quinones; D014812:Vitamin K; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D002256:Carbonic Anhydrases