title: Characterization of mutants with single and multiple defects in the tryptophan biosynthetic pathway in Bacillus subtilis.
abstract: Sixty-five tryptophan auxotrophs which map in a cluster on the genome of Bacillus subtilis were characterized on the basis of (i) growth response, (ii) accumulation of intermediate compounds, and (iii) determination of enzymatic defects. They could be placed into six phenotypic classes. Certain of the mutants exhibited pleiotropic effects on more than one enzymatic activity in a manner different from those effects reported for the tryptophan pathway in other organisms. Invariably, mutations in the second gene, that coding for phosphoribosyl transferase activity, were found to lack the indoleglycerol phosphate synthase activity specified by the third gene in the cluster; however, this polarity did not extend to genes more distal in the cluster. Furthermore, mutations in the gene which codes for phosphoribosyl-anthranilate isomerase not only led to a loss of this enzyme but also to a loss of phosphoribosyl transferase and indoleglycerol phosphate synthase. In contrast, mutations in either of the loci coding for these latter functions had no apparent effect on isomerase activity. No polarity of the conventional type was found, e.g., none of the mutations in any gene led to polarized effects on the levels of the enzymes specified by the other genes of the cluster. These observations indicated a possible in vivo aggregation involving the transferase, isomerase, and synthase enzymes, with the isomerase acting as the "key" enzyme in the aggregate.
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology* / Q000378:metabolism; D006836:Hydro-Lyases / Q000378:metabolism; D007211:Indoles / Q000378:metabolism / Q000494:pharmacology; D007535:Isomerases / Q000378:metabolism; D008967:Molecular Biology; D009154:Mutation; D014166:Transferases / Q000378:metabolism; D014364:Tryptophan / Q000096:biosynthesis* / Q000494:pharmacology; D062367:ortho-Aminobenzoates / Q000378:metabolism / Q000494:pharmacology
chemical_list: D007211:Indoles; D062367:ortho-Aminobenzoates; D014364:Tryptophan; D014166:Transferases; D006836:Hydro-Lyases; D007535:Isomerases