title: Role of pyruvate metabolism in the growth of Streptococcus faecalis in the presence of propionate.
abstract: The growth of Streptococcus faecalis is inhibited by propionate, and the inhibition is reversed by lipoic acid or acetate. A study of the role of pyruvate oxidation in S. faecalis showed that propionate inhibited the lipoic acid-dependent aerobic oxidation of pyruvate in resting cells. Pyruvate dehydrogenation with neotetrazolium as a hydrogen acceptor in cell-free extracts also required lipoic acid and was markedly inhibited by propionyl phosphate as well as sodium propionate. Some lipid substances, such as palmitate, oleate, behenate, and lecithin, had a lipoic acid-replacing effect on growth of the organism. Biotin or bicarbonate promoted the lipoic acid-dependent growth. Acetate-2-(14)C added to the medium was mainly incorporated into the lipid fraction of the cells. Evolution of (14)CO(2) from pyruvate-2-(14)C was not observed in resting cells of the organism, even under aerobic conditions. From the above findings, it is concluded that lipid synthesis through pyruvate oxidation plays a very important role in bacterial growth in medium containing propionate.
mesh_terms: D000085:Acetates / Q000494:pharmacology; D001639:Bicarbonates / Q000494:pharmacology; D001710:Biotin / Q000494:pharmacology; D002245:Carbon Dioxide / Q000378:metabolism; D002247:Carbon Isotopes; D013293:Enterococcus faecalis / Q000254:growth & development*; D008055:Lipids / Q000494:pharmacology; D009829:Oleic Acids / Q000494:pharmacology; D010100:Oxygen; D010169:Palmitic Acids / Q000494:pharmacology; D010713:Phosphatidylcholines / Q000494:pharmacology; D011422:Propionates / Q000494:pharmacology*; D011773:Pyruvates / Q000378:metabolism*; D013778:Tetrazolium Salts / Q000494:pharmacology; D008063:Thioctic Acid / Q000494:pharmacology
chemical_list: D000085:Acetates; D001639:Bicarbonates; D002247:Carbon Isotopes; D008055:Lipids; D009829:Oleic Acids; D010169:Palmitic Acids; D010713:Phosphatidylcholines; D011422:Propionates; D011773:Pyruvates; D013778:Tetrazolium Salts; D002245:Carbon Dioxide; D001710:Biotin; D008063:Thioctic Acid; D010100:Oxygen