title: Autolysis and secondary growth of Mycobacterium tuberculosis in submerged culture.
abstract: Mycobacterium tuberculosis has been found to exhibit autolysis and spontaneous secondary growth in modified Sauton medium. The phenomenon is a function of high glycerol concentration of the medium and limitation of air in test tubes. It is not a function of depletion of nutrients and is probably not a manifestation of lysogeny or spheroplast formation. The cycle of growth, autolysis, and secondary growth is related to development of lethal conditions during a period of severe oxygen limitation followed by a metabolic change associated with slower depletion of glycerol.
mesh_terms: D001433:Bacteriolysis; D003470:Culture Media; D005990:Glycerol / Q000494:pharmacology*; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000254:growth & development*; D009584:Nitrogen / Q000378:metabolism; D010100:Oxygen / Q000494:pharmacology
chemical_list: D003470:Culture Media; D009584:Nitrogen; D005990:Glycerol; D010100:Oxygen