Transducing bacteriophage for Bacillus cereus.
A phage, designated CP-51, that carries out generalized transduction in Bacillus cereus 569 was isolated from soil. All auxotrophic mutants tested, those requiring tryptophan, histidine, leucine, isoleucine, methionine, or phenylalanine, were transduced to prototrophy. The phage was extremely unstable when stored at 2 to 4 C, but stability was enhanced by storage at higher temperatures. The optimal temperature of those tested for maintenance of plaque-forming units was 15 C.
D000596:Amino Acids; D001409:Bacillus cereus; D001435:Bacteriophages / Q000302:isolation & purification* / Q000528:radiation effects; D003588:Cytopathogenic Effect, Viral; D006358:Hot Temperature; D007106:Immune Sera / Q000494:pharmacology; D009154:Mutation; D011830:Radiation Effects; D012988:Soil Microbiology; D013696:Temperature; D014161:Transduction, Genetic; D014466:Ultraviolet Rays
D000596:Amino Acids; D007106:Immune Sera