The aminoethylphosphonate-containing lipids of rumen protozoa.
1. A method is presented for identifying and estimating the aminoethylphosphonate (ciliatine)-containing phospholipids in a complex mixture. 2. Evidence was obtained that the phospholipids of a pure culture of Entodinium caudatum and a mixed rumen protozoa sample contain diglyceride ciliatine, and a plasmalogen ciliatine was detected in the latter. 3. A ninhydrin-positive sphingolipid was isolated from rumen protozoa. Although chromatographically homogeneous on silica gel it contains two components, which were provisionally identified as ceramide ciliatine and ceramide phosphorylethanolamine. 4. A detailed phospholipid analysis of E. caudatum and rumen protozoa is presented. They contain no phosphatidylserine or cardiolipin, but an unidentified phosphoglyceride containing a zwitterionic amino acid is present.
D000818:Animals; D002855:Chromatography, Thin Layer; D016798:Ciliophora / Q000032:analysis*; D005989:Glycerides / Q000032:analysis; D063065:Organophosphonates / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis*; D010955:Plasmalogens / Q000032:analysis; D012417:Rumen / Q000382:microbiology*; D012756:Sheep
D005989:Glycerides; D063065:Organophosphonates; D010743:Phospholipids; D010955:Plasmalogens