Lytic effects of staphylococcal alpha-toxin and delta-hemolysin.
Several preparations of staphylococcal alpha-toxin and delta-lysin were studied in order to compare hemolytic activity with capacity to lyse bacterial protoplasts. delta-Lysin in relatively low concentration lysed protoplasts of Sarcina lutea, protoplasts of Streptococcus faecalis, and spheroplasts of Escherichia coli. Lysis of bacterial protoplasts by preparations of alpha-toxin appeared to be due to contamination of the preparations with delta-lysin. Data comparing the protoplast-lysing activity of various lytic agents are presented.
D001769:Blood; D002499:Centrifugation, Density Gradient; D013293:Enterococcus faecalis / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D006460:Hemolysin Proteins / Q000494:pharmacology*; D010713:Phosphatidylcholines / Q000494:pharmacology; D011523:Protoplasts / Q000187:drug effects*; D012505:Sarcina / Q000187:drug effects; D013210:Staphylococcus; D014118:Toxins, Biological / Q000032:analysis / Q000494:pharmacology*
D006460:Hemolysin Proteins; D010713:Phosphatidylcholines; D014118:Toxins, Biological