Separation of replicative intermediate from single-stranded ribonucleic acid by sedimentation at low ionic strength.
By sedimentation in 2.5 x 10(-4)m sodium ethylenediaminetetraacetate at 25 C, replicative intermediate could be separated from single-stranded ribonucleic acid, both viral and ribosomal. All of the label in the replicative intermediate after a brief pulse of titrated uridine was resistant to ribonuclease.
D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D002621:Chemistry; D003090:Coliphages; D004492:Edetic Acid; D004926:Escherichia coli / Q000378:metabolism; D009691:Nucleic Acid Denaturation; D012329:RNA, Bacterial / Q000302:isolation & purification*; D012367:RNA, Viral / Q000302:isolation & purification*; D012260:Ribonucleases; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D012329:RNA, Bacterial; D012367:RNA, Viral; D014316:Tritium; D004492:Edetic Acid; D012260:Ribonucleases; D014529:Uridine