title: The 5'-nucleotidases and cyclic phosphodiesterases (3'-nucleotidases) of the Enterobacteriaceae.
abstract: All members of the Enterobacteriaceae possess distinct 5'-nucleotidases and cyclic phosphodiesterases (3'-nucleotidases) that can be differentiated from the acid and alkaline phosphatases and the acid sugar hydrolases. The nucleotidases and cyclic phosphodiesterases of the various Enterobacteriaceae are remarkably similar in properties. All of the 5'-nucleotidases hydrolyze 5'-nucleotides, adenosine triphosphate, and uridine diphosphoglucose. Their pH optimum is from 5.7 to 6.1. The cyclic phosphodiesterases hydrolyze 3'-nucleotides, cyclic phosphonucleotides, bis-(p-nitrophenyl)phosphate, and p-nitrophenylphosphate. Their pH optimum is from 7.2 to 7.8. For both enzymes, cobalt showed optimal metal stimulation. An intracellular protein inhibitor for the 5'-nucleotidase is present in all of the Enterobacteriaceae. No inhibitor of cyclic phosphodiesterase activity exists, although hydrolysis of both cyclic phosphonucleotides and 3'-nucleotides is inhibited by ribonucleic acid. Neither of the enzymes is subject to control by phosphate level or by catabolite repression. Of the other bacteria studied, only Haemophilus and Bacillus subtilis contained significant 3'- or 5'-nucleotidase activity.
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology; D002118:Calcium / Q000494:pharmacology; D003035:Cobalt / Q000494:pharmacology; D004754:Enterobacter / Q000201:enzymology; D004755:Enterobacteriaceae / Q000201:enzymology*; D004925:Escherichia / Q000201:enzymology; D004926:Escherichia coli / Q000201:enzymology; D006193:Haemophilus influenzae / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D009708:Nucleotidases; D010710:Phosphates / Q000494:pharmacology; D011511:Proteus / Q000201:enzymology; D012475:Salmonella / Q000201:enzymology; D012706:Serratia marcescens / Q000201:enzymology; D012760:Shigella / Q000201:enzymology
chemical_list: D010710:Phosphates; D003035:Cobalt; D008345:Manganese; D009708:Nucleotidases; D008274:Magnesium; D002118:Calcium