title: Effect of different nutritional conditions on the synthesis of tricarboxylic acid cycle enzymes.
abstract: The effect of various nutritional conditions on the levels of Krebs cycle enzymes in Bacillus subtilis, B. licheniformis, and Escherichia coli was determined. The addition of glutamate, alpha-ketoglutarate, or compounds capable of being catabolized to glutamate, to a minimal glucose medium resulted in complete repression of aconitase in B. subtilis and B. licheniformis. The synthesis of fumarase, succinic dehydrogenase, malic dehydrogenase, and isocitric dehydrogenase was not repressed by these compounds. It is postulated that glutamate or alpha-ketoglutarate is the true corepressor for the repression of aconitase. A rapidly catabolizable carbon source and alpha-ketoglutarate or glutamate must be simultaneously present for complete repression of the formation of aconitase. Conditions which repress the synthesis of aconitase in B. subtilis restrict the flow of carbon in the sequence of reactions leading to alpha-ketoglutarate but do not prevent glutamate oxidation in vivo. The data indicate that separate and independent mechanisms regulate the activity of the anabolic and catabolic reactions of the Krebs cycle in B. subtilis and B. licheniformis. The addition of glutamate to the minimal glucose medium results in the repression of aconitase, isocitric dehydrogenase, and fumarase, but not malic dehydrogenase in E. coli K-38.
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D001412:Bacillus subtilis / Q000201:enzymology*; D002247:Carbon Isotopes; D002952:Citric Acid Cycle / Q000187:drug effects*; D003470:Culture Media / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology*; D005947:Glucose / Q000378:metabolism; D005971:Glutamates / Q000494:pharmacology*; D006836:Hydro-Lyases / Q000378:metabolism*; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D013170:Spores
chemical_list: D002247:Carbon Isotopes; D003470:Culture Media; D005971:Glutamates; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D006836:Hydro-Lyases; D005947:Glucose