Alkaline inorganic pyrophosphatase activity of mammalian-cell alkaline phosphatase.
Alkaline phosphatase prepared from mammalian cell cultures was found to have alkaline inorganic pyrophosphatase activity. Both of these activities appear to be associated with a single protein, as demonstrated by: (1) concomitant purification of alkaline phosphatase and alkaline inorganic pyrophosphatase; (2) proportional precipitation of alkaline phosphatase and inorganic pyrophosphatase activities by titrating constant amounts of an enzyme preparation with increasing concentration of antibody; (3) immune electrophoresis, which showed that precipitin bands that have alkaline phosphatase activity also have pyrophosphatase activity; (4) inhibition of pyrophosphatase activity by cysteine, an inhibitor of alkaline phosphatase activity; (5) similar subcellular localization of the two enzyme activities as demonstrated by histochemical methods; (6) hormonal and substrate induction of alkaline phosphatase activity in mammalian cell cultures, which produced a nearly parallel rise in inorganic pyrophosphatase activity.
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D003470:Culture Media; D004790:Enzyme Induction; D000882:Haplorhini; D006367:HeLa Cells / Q000166:cytology / Q000201:enzymology*; D007122:Immunoelectrophoresis; D007668:Kidney / Q000166:cytology; D010710:Phosphates / Q000494:pharmacology; D011233:Precipitin Tests; D011239:Prednisolone / Q000494:pharmacology; D011755:Pyrophosphatases / Q000378:metabolism*
D003470:Culture Media; D010710:Phosphates; D011239:Prednisolone; D000469:Alkaline Phosphatase; D011755:Pyrophosphatases