The essentiality of histidine in the catalytic action of subtilisin. Covalent modification by a specific reagent.
D000478:Alkylation; D001412:Bacillus subtilis / Q000201:enzymology*; D006639:Histidine; D007659:Ketones; D010447:Peptide Hydrolases; D010649:Phenylalanine
D007659:Ketones; D010649:Phenylalanine; D006639:Histidine; D010447:Peptide Hydrolases