The alk-1-enyl group content of mammalian myelin phosphoglycerides by quantitative two-dimensional thin-layer chromatography.
Myelin phospholipids have been examined by a separation-reaction-separation procedure for two-dimensional thin-layer chromatography on silica gel. After separation in one dimension, alk-1-enyl groups are cleaved by exposure of the plates to HCl fumes. Development in the second dimension quantitatively separates acid-labile and acid-stable phosphoglycerides as well as the aldehydes released from the acid-labile phosphoglycerides. Myelin phospholipids from the central nervous systems of the rhesus monkey, squirrel monkey, ox, and mouse contain 32-36% acid-labile ethanolamine phosphoglycerides (ethanolamine plasmalogens) and 8-14% acid-stable ethanolamine phosphoglycerides. Acid-labile choline and serine phosphoglycerides account for less than 1% of the myelin phospholipids.
D000475:Alkenes / Q000032:analysis*; D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002490:Central Nervous System / Q000032:analysis*; D002855:Chromatography, Thin Layer; D003337:Corpus Callosum / Q000032:analysis; D004355:Drug Stability; D005989:Glycerides; D000882:Haplorhini; D006863:Hydrogen-Ion Concentration; D008526:Medulla Oblongata / Q000032:analysis; D051379:Mice; D008861:Microsomes / Q000032:analysis; D009186:Myelin Sheath / Q000032:analysis*; D009900:Optic Nerve / Q000032:analysis; D010714:Phosphatidylethanolamines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*; D010955:Plasmalogens; D013116:Spinal Cord / Q000032:analysis
D000475:Alkenes; D005989:Glycerides; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D010955:Plasmalogens