Conversion of mesophilic to psychrophilic bacteria.
The minimum temperature of growth of the mesophilic bacterium Pseudomonas aeruginosa has been significantly lowered from approximately 11 degrees to 0 degrees C. This shift in the minimum temperature of growth was accompanied by a corresponding decrease in the maximum temperature of growth. Transfer of this genetic characteristic by a transducing phage grown on a psychrophile or by ultraviolet mutagenesis was used to accomplish these shifts in range of growth temperature.
D001435:Bacteriophages; D003080:Cold Temperature; D003470:Culture Media; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000254:growth & development*; D011831:Radiation Genetics; D013696:Temperature; D014161:Transduction, Genetic; D014364:Tryptophan / Q000378:metabolism; D014466:Ultraviolet Rays
D003470:Culture Media; D014364:Tryptophan