Enzymatic cleabage and repair of transforming DNA.
Single-strand nicks caused by DNase I are capable of inactivating B. subtilis transforming DNA. Under suitable conditions, the polynucleotide joining enzyme from E. coli and a similar DPN-requiring activity from B. subtilis can completely repair this damage, restore biological activity, and increase the single-strand molecular weight. The rates of inactivation of a single genetic marker and of a four-marker linkage group suggest that a single-strand nick is inactivating even when far from the site of genetic damage.
D001412:Bacillus subtilis; D004269:DNA, Bacterial; D003851:Deoxyribonucleases; D004926:Escherichia coli; D008025:Ligases; D014170:Transformation, Genetic; D014316:Tritium
D004269:DNA, Bacterial; D014316:Tritium; D003851:Deoxyribonucleases; D008025:Ligases