Methods for the separation and identification of mycobactins from various species of mycobacteria.
A single pure component was isolated from mycobactin P by countercurrent distribution; its side chain is n-cis-octadec-2-enoyl; its purity and molecular structure were confirmed by mass spectrometry of its aluminium complex. The separation of ferric and of aluminium complexes of mycobactins by thin-layer chromatography is described. Mycobacterium terrae, M. marinum and M. smegmatis produce mycobactins that differ among themselves and from mycobactins P and T. A nomenclature for the mycobactins and their derivatives is suggested.
D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D003377:Countercurrent Distribution; D003470:Culture Media; D005227:Fatty Acids / Q000032:analysis; D006133:Growth Substances / Q000032:analysis*; D009161:Mycobacterium / Q000032:analysis*; D009169:Mycobacterium tuberculosis / Q000032:analysis*; D013045:Species Specificity; D013053:Spectrophotometry; D013057:Spectrum Analysis; D009626:Terminology as Topic
D003470:Culture Media; D005227:Fatty Acids; D006133:Growth Substances