title: Sporulation mutations induced by heat in Bacillus subtilis.
abstract: When spores of Bacillus subtilis strain Marburg are heated (90 degrees to 100 degrees C) in a vacuum for 9 to 12 hours and then plated, numerous mutants are obtained, and very few spores are killed. Disproportionately large numbers of these mutants exhibit abnormal sporulation.
mesh_terms: D001412:Bacillus subtilis; D003470:Culture Media; D006358:Hot Temperature; D009154:Mutation; D013170:Spores
chemical_list: D003470:Culture Media