Gene-enzyme relationships in Neurospora invertase.
A spontaneous, single-gene mutation responsible for a total lack of invertase activity in Neurospora crassa is described. The mutation is believed to lie in the structural gene for invertase, since an immunologically cross-reacting protein is made by the mutant strain. In addition, there was no evidence for a defect in regulation of invertase activity or synthesis by the following criteria. (i) The invertaseless condition was recessive in heterokaryons; (ii) no invertase inhibitor was found in mutant extracts by mixing experiments; and (iii) none of the several sugars able to induce activity in wild-type strains was able to induce activity in the mutant strain. It was also discovered that most of the wild-type enzyme (55 to 75%) cannot be washed free from the rapidly sedimenting cell debris. This finding provided additional support for the hypothesis that Neurospora invertase is located within or about the cell wall.
D002473:Cell Wall / Q000201:enzymology; D004790:Enzyme Induction; D005796:Genes; D005827:Genetics, Microbial; D006026:Glycoside Hydrolases / Q000032:analysis; D007120:Immunochemistry; D005779:Immunodiffusion; D008967:Molecular Biology; D009154:Mutation; D009491:Neurospora / Q000032:analysis / Q000201:enzymology*
D006026:Glycoside Hydrolases