In vivo interaction of endotoxin with a plasma lipoprotein having esterase activity.
Circulating endotoxin was specifically precipitated from plasma samples withdrawn from three different animal species subsequent to parenteral injection of the toxin. Lipoprotein-positive staining and esterase activity were demonstrated on the precipitation lines formed in immunodiffusion, thus establishing the in vivo interaction of endotoxin with a plasma lipoprotein having esterase activity. Evidence was given to show that the intensity of this interaction in circulating plasma increased gradually with time. The concordance of this in vivo inter-action with the in vitro degradation and inactivation of endotoxin by plasma esterases is discussed.
D000818:Animals; D004731:Endotoxins / Q000378:metabolism*; D004950:Esterases / Q000097:blood* / Q000378:metabolism; D006168:Guinea Pigs; D005779:Immunodiffusion; D008074:Lipoproteins / Q000097:blood* / Q000378:metabolism; D051379:Mice; D011817:Rabbits; D012475:Salmonella
D004731:Endotoxins; D008074:Lipoproteins; D004950:Esterases