title: The demonstration of the succinic dehydrogenase system in Bacillus subtilis using tetranitro--blue tetrazolium combined with techniques of electron microscopy.
abstract: Activity of the succinic dehydrogenase system was studied in Bacillus subtilis utilizing combined techniques of cytochemistry and electron microscopy. Organisms were incubated in a medium containing tetranitro-blue tetrazolium (TNBT) which served as an electron acceptor. Enzymatic activity, as evidenced by deposition of TNBT-formazan, was found on membranous organelles associated with the cytoplasmic membrane and septal plasma membrane, the nuclear area, and the plasma membrane. Flagella, approximately 190 A in diameter, with thorn-like projections protruded through the cell wall. Tangential-oblique sections of the cell wall showed many pores approximately 220 A in diameter with a center-to-center spacing of approximately 450 A.
mesh_terms: D001412:Bacillus subtilis / Q000166:cytology* / Q000201:enzymology*; D004396:Coloring Agents; D006651:Histocytochemistry; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D013385:Succinate Dehydrogenase / Q000378:metabolism*
chemical_list: D004396:Coloring Agents; D013385:Succinate Dehydrogenase