L-tyrosine-3,5-3H assay for tyrosinase development in skin of newborn hamsters.
Tyrosinase in hamster skin, measured by the production of (3)HOH from L-tyrosine-3,5-(3)H, increases about sevenfold in specific activity from the 14-day fetus to day 6 of life. Albino hamsters exhibit no activity, but enzyme is present in the ventral (white) areas of the Syrian hamster. 3,4-Dihydroxy-L-phenylalanine is required as a cosubstrate.
D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D004156:Catechol Oxidase / Q000032:analysis* / Q000378:metabolism; D006224:Cricetinae; D005333:Fetus; D012867:Skin / Q000201:enzymology*; D014316:Tritium; D014443:Tyrosine / Q000378:metabolism*
D014316:Tritium; D014443:Tyrosine; D004156:Catechol Oxidase