title: Soluble antigens obtained from influenza virus by treatment with non-ionic detergent.
abstract: Highly purified influenza virus was degraded using anionic and non-ionic detergents. Best results were obtained using the non-ionic detergent Triton N 101. Tests showed that virus extracts contained neuraminidase and a substance that reacted specifically with rabbit antibody to virus haemagglutinin (specific serum blocking substance). Haemagglutination-inhibiting antibody was produced when virus extracts were inoculated into guinea-pigs. Immunodiffusion tests showed that extracts were complex. Host-specific material was regularly found. Under appropriate conditions S-antigen was detected as a single line pattern component. Two or more virus-specific materials were also present. One of these was probably neuraminidase and the other the specific serum blocking substance.
mesh_terms: D000818:Animals; D000941:Antigens / Q000032:analysis*; D002642:Chick Embryo; D006168:Guinea Pigs; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D007106:Immune Sera; D005779:Immunodiffusion; D009439:Neuraminidase / Q000032:analysis; D009975:Orthomyxoviridae / Q000032:analysis* / Q000201:enzymology; D011817:Rabbits; D013045:Species Specificity; D013053:Spectrophotometry; D013501:Surface-Active Agents
chemical_list: D000941:Antigens; D007106:Immune Sera; D013501:Surface-Active Agents; D009439:Neuraminidase