Production of L-arginine by arginine hydroxamate-resistant mutants of Bacillus subtilis.
l-Arginine hydroxamate inhibited the growth of various bacteria, and the inhibition was readily reversed by arginine. l-Arginine hydroxamate (10(-3)m) completely inhibited the growth of Bacillus subtilis. This inhibitory effect was prevented by 2.5 x 10(-4)ml-arginine, which was the most effective of all the natural amino acids in reversing the inhibition. l-Arginine hydroxamate-resistant mutants of Bacillus subtilis were isolated and found to excrete l-arginine in relatively high yields. One of the mutants, strain AHr-5, produced 4.5 mg of l-arginine per ml in shaken culture in 3 days.
D001120:Arginine / Q000096:biosynthesis* / Q000378:metabolism / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D002854:Chromatography, Paper; D004352:Drug Resistance, Microbial; D005285:Fermentation; D006877:Hydroxamic Acids / Q000494:pharmacology; D009154:Mutation; D013237:Stereoisomerism
D006877:Hydroxamic Acids; D001120:Arginine