Morphology of a virus of blue-green algae and properties of its deoxyribonucleic acid.
The morphology of Safferman's virus of blue-green algae (phycovirus LPP-1) has been studied by electron microscopy and physicochemical methods. The virion has a short (100 to 200 A long, 150 A in diameter) forked tail, with an outer sheath, an inner core, and a capital attached to one of the vertices of a polyhedral head. The head capsid edge-to-edge distance is 600 A, based upon internal calibration of the magnification in electron micrographs by use of the line-line spacing of catalase crystals. Measurements of absorbancy and infectivity, and electron microscopy across the band of virus after zone centrifugation on a sucrose gradient, indicated that infectivity was correlated with the short-tailed particles described. The viral deoxyribonucleic acid (DNA) is linear, with a contour length of 13.2 +/- 0.5 mu, measured by the Kleinschmidt method. Its sedimentation coefficient, S(0) (20, w), is 33.4 +/- 0.7 S. These values are consistent with a molecular weight of 27 x 10(6) for the viral DNA. Based upon buoyant density in CsCl and thermal denaturation, the guanine-cytosine content of the DNA is 53%. The viral DNA was used as template for in vitro ribonucleic acid (RNA) synthesis by Escherichia coli RNA polymerase. This RNA annealed to 18% of the sequences in the viral DNA, 0.5% of the sequences in bacteriophage T7 DNA, and 0.25% of the sequences in Plectonema boryanum DNA, at saturating levels of RNA in the Hall-Nygaard hybridization assay. The lack of homology with T7 DNA is of interest because the two viruses are very similar morphologically. The lack of homology with host DNA suggests that this algal virus is a poor candidate for transduction.
D001435:Bacteriophages / Q000254:growth & development / Q000302:isolation & purification; D002374:Catalase; D002499:Centrifugation, Density Gradient; D000458:Cyanobacteria; D003596:Cytosine; D004279:DNA, Viral; D006147:Guanine; D006462:Hemolytic Plaque Technique; D006824:Hybridization, Genetic; D008854:Microscopy, Electron; D008970:Molecular Weight; D010942:Plant Viruses / Q000254:growth & development / Q000302:isolation & purification
D004279:DNA, Viral; D006147:Guanine; D003596:Cytosine; D002374:Catalase