Isolation of normal immunoglobulin E by means of an immunoadsorbent.
An improved method for the isolation of normal immunoglobulin E is described. The method is based on the use of an immunoadsorbent formed by the mechanical entrapment of antibodies against a myeloma immunoglobulin E into a lattice of a highly cross-linked macroporous polyacrylamide gel. Normal immunoglobulin E was isolated from an immunoglobulin E-rich serum pool as well as from an individual healthy donor. The isolated immunoglobulin E was contaminated with about 20% of immunoglobulin G. An antiserum against normal immunoglobulin E was prepared by immunizing rabbits with the isolated immunoglobulin E.
D000178:Acrylamides; D000179:Acrylates; D000327:Adsorption; D000818:Animals; D001536:Bence Jones Protein / Q000302:isolation & purification; D044383:Black People; D002852:Chromatography, Ion Exchange; D003911:Dextrans; D004586:Electrophoresis; D006801:Humans; D007106:Immune Sera; D005779:Immunodiffusion; D007073:Immunoglobulin E / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000302:isolation & purification; D007136:Immunoglobulins / Q000302:isolation & purification*; D009101:Multiple Myeloma / Q000276:immunology; D011817:Rabbits; D012756:Sheep; D013552:Swine; D044465:White People
D000178:Acrylamides; D000179:Acrylates; D003911:Dextrans; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D007073:Immunoglobulin E; D001536:Bence Jones Protein