Procedures for the assay of carbenicillin in body fluids.
The assay of carbenicillin in clinical specimens is complicated by the fact that carbenicillin also contains a small amount of benzylpenicillin, thereby precluding the use of conventional penicillin assay organisms. This report gives details of a microbiological assay method involving the use of a strain of Pseudomonas aeruginosa which is very sensitive to carbenicillin but insensitive to benzylpenicillin. The outline of a microassay method with this organism is presented, and a method for the assay of specimens containing mixtures of carbenicillin and other antibiotics is described.
D000362:Agar; D001412:Bacillus subtilis / Q000187:drug effects; D001681:Biological Assay; D055598:Chemical Phenomena; D002621:Chemistry; D003352:Corynebacterium / Q000187:drug effects; D008722:Methods; D008826:Microbial Sensitivity Tests; D010405:Penicillinase / Q000494:pharmacology; D010406:Penicillins / Q000032:analysis* / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D012505:Sarcina / Q000187:drug effects
D010406:Penicillins; D000362:Agar; D010405:Penicillinase