Purification and properties of L-asparaginase from Serratia marcescens.
The purification and properties of a tumor inhibitory l-asparaginase from Serratia marcescens are described. The following properties of the enzyme were examined: kinetics of the enzyme reaction, catalytic activity as a function of pH, boundary sedimentation velocity, electrophoresis on polyacrylamide gel, immuno-electrophoresis against homologous and heterologous antisera, immunodiffusion, blood clearance rate in mice, and inhibition of the 6C3HED lymphoma in C3H mice. Complete regression of this tumor was obtained with a smaller dose of the enzyme from S. marcescens than with enzyme from Escherichia coli. The reason for this difference was not evident from a comparison of several properties of the two enzymes.
D000179:Acrylates; D000641:Ammonia / Q000096:biosynthesis; D000818:Animals; D001215:Asparaginase / Q000032:analysis / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism / Q000627:therapeutic use; D001216:Asparagine / Q000378:metabolism; D002848:Chromatography, DEAE-Cellulose; D003429:Cross Reactions; D003470:Culture Media; D004589:Electrophoresis, Disc; D004926:Escherichia coli / Q000201:enzymology / Q000276:immunology; D005260:Female; D005782:Gels; D006863:Hydrogen-Ion Concentration; D006882:Hydroxyapatites; D007106:Immune Sera; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007274:Injections, Intraperitoneal; D008228:Lymphoma, Non-Hodgkin / Q000097:blood / Q000188:drug therapy; D051379:Mice; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000097:blood; D011817:Rabbits; D012513:Sarcoma, Experimental / Q000188:drug therapy; D012706:Serratia marcescens / Q000201:enzymology* / Q000254:growth & development / Q000276:immunology / Q000378:metabolism; D013045:Species Specificity; D013053:Spectrophotometry; D014461:Ultracentrifugation
D000179:Acrylates; D003470:Culture Media; D005782:Gels; D006882:Hydroxyapatites; D007106:Immune Sera; D001216:Asparagine; D000641:Ammonia; D001215:Asparaginase