title: Effect of the method of agitation on the accumulation of protective antigen in cultures of Bacillus anthracis.
abstract: Accumulation of protective antigen was essentially independent of culture volume, provided the organisms were kept in suspension by stirring. Agitation by orbited shaking was unsatisfactory.
mesh_terms: D000693:Anaerobiosis; D000941:Antigens / Q000378:metabolism*; D000942:Antigens, Bacterial / Q000378:metabolism; D001408:Bacillus anthracis / Q000254:growth & development / Q000276:immunology*; D001431:Bacteriological Techniques; D003168:Complement Fixation Tests; D003470:Culture Media; D005779:Immunodiffusion
chemical_list: D000941:Antigens; D000942:Antigens, Bacterial; D003470:Culture Media