Nucleic acid homology in the genus Mycobacterium.
Immobilized deoxyribonucleic acid (DNA) preparations from eight species of mycobacteria were reacted with labeled reference DNA from Mycobacterium tuberculosis and M. kansasii. All mycobacteria showed some degree of homology. Immobilized DNA from Pseudomonas multivorans, which has a guanine plus cytosine content within the range established for the mycobacteria, showed no significant binding with either reference system. Relative per cent binding and thermal stability of bound DNA were used as parameters for determining degrees of relatedness among members of the genus. When these relationships were compared with those established by numberical taxonomic methods, a high correlation was found.
D002247:Carbon Isotopes; D004269:DNA, Bacterial / Q000302:isolation & purification; D005827:Genetics, Microbial; D006358:Hot Temperature; D006824:Hybridization, Genetic; D009161:Mycobacterium / Q000032:analysis / Q000145:classification*; D009169:Mycobacterium tuberculosis / Q000032:analysis; D009691:Nucleic Acid Denaturation; D011549:Pseudomonas / Q000032:analysis; D013045:Species Specificity; D013053:Spectrophotometry; D014498:Uracil
D002247:Carbon Isotopes; D004269:DNA, Bacterial; D014498:Uracil