Characterization of ribonucleic acid from visna virus.
A single-stranded ribonucleic acid(s) has been isolated from purified virions of visna virus. It consists of two major components, namely 63S and "4S," under the conditions employed for ribonucleic acid (RNA) extraction. The 63S component can be converted to subunits by heat and dimethylsulfoxide treatments. Analyses by base composition indicate that the "4S" RNA isolated from visna virus is not a random breakdown product of the 63S component as a result of extraction, nor is it randomly derived from cellular RNA.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002831:Choroid Plexus; D046508:Culture Techniques; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D006358:Hot Temperature; D010756:Phosphoric Acids; D010759:Phosphorus Isotopes; D011188:Potassium; D011328:Prions / Q000032:analysis* / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D012756:Sheep; D013644:Tartrates; D014316:Tritium; D014529:Uridine; D014776:Virus Cultivation
D010756:Phosphoric Acids; D010759:Phosphorus Isotopes; D011328:Prions; D012367:RNA, Viral; D013644:Tartrates; D014316:Tritium; D011188:Potassium; D014529:Uridine; D004121:Dimethyl Sulfoxide