DNA polymerase activities in varions of visna virus, a causative agent of a "slow" neurological disease.
The presence is reported of an RNA-instructed DNA polymerase in visna virus, the causative agent of a "slow" neurological disease in sheep. The product synthesized by the RNA-directed reaction has been shown to be a DNA heteropolymer by the following criteria: synthesis requires the presence of all four deoxyriboside triphosphates; the product is resistant to ribonuclease and alkali but is degraded by DNase; and the product has a density of 1.420 in Cs(2)SO(4) solution, characteristic of DNA.Visna virions, like those of the oncogenic RNA viruses, contain DNA polymerase activities that respond to a variety of double-stranded DNAs and to synthetic DNA.RNA hybrids.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002460:Cell Line / Q000382:microbiology; D002499:Centrifugation, Density Gradient; D002831:Choroid Plexus / Q000382:microbiology; D003588:Cytopathogenic Effect, Viral; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004279:DNA, Viral / Q000032:analysis; D006824:Hybridization, Genetic; D007700:Kinetics; D009711:Nucleotides / Q000378:metabolism; D009858:Oncogenic Viruses / Q000201:enzymology; D011328:Prions / Q000201:enzymology*; D012367:RNA, Viral / Q000378:metabolism; D012608:Scrapie / Q000382:microbiology; D012756:Sheep; D012897:Slow Virus Diseases / Q000382:microbiology; D013698:Templates, Genetic; D014316:Tritium
D004279:DNA, Viral; D009711:Nucleotides; D011328:Prions; D012367:RNA, Viral; D014316:Tritium; D000255:Adenosine Triphosphate; D004254:DNA Nucleotidyltransferases