title: Cell wall binding properties of the Bacillus subtilis autolysin(s).
abstract: Cell walls isolated from exponentially growing Bacillus subtilis have autolysin(s) attached to them. An autolysin can be released from the walls by incubation at 0 C with 3 m LiCl. The enzyme can reattach to walls when the salt concentration is reduced. The bound enzyme cannot be removed or destroyed by washing the walls with 8 m urea at 0 C. The binding of free enzyme to walls at 0 C can take place normally in the presence of 2 m urea.
mesh_terms: D001329:Autolysis; D001412:Bacillus subtilis / Q000187:drug effects / Q000201:enzymology*; D001433:Bacteriolysis; D002473:Cell Wall / Q000201:enzymology*; D002712:Chlorides / Q000494:pharmacology; D006867:Hydrolases / Q000302:isolation & purification*; D008094:Lithium / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology
chemical_list: D002712:Chlorides; D014508:Urea; D008094:Lithium; D006867:Hydrolases