title: Chemical characterization of a new surface antigenic polysaccharide from a mutant of Staphylococcus aureus.
abstract: A mutant of Staphylococcus aureus H was isolated by virtue of its inability to agglutinate with antibodies against teichoic acid of S. aureus. Immunological studies revealed that the mutant, S. aureus T, possessed a new surface antigen in addition to having the antigenic determinant of the wild-type strain, the ribitol teichoic acid. The presence of this additional surface component rendered strain T resistant to staphylococcal typing phages, presumably by masking the phage-receptor sites. The polymer was separated from teichoic acid by chromatography on diethylaminoethyl cellulose and was shown to be composed of two amino sugars, N-acetyl-d-fucosamine and N-acetyl-d-mannosamin uronic acid.
mesh_terms: D000372:Agglutination Tests; D000606:Amino Sugars / Q000032:analysis; D000942:Antigens, Bacterial / Q000032:analysis / Q000302:isolation & purification; D002473:Cell Wall / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002848:Chromatography, DEAE-Cellulose; D002854:Chromatography, Paper; D003090:Coliphages; D005827:Genetics, Microbial; D005944:Glucosamine / Q000032:analysis; D006595:Hexosamines / Q000032:analysis; D007106:Immune Sera; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007202:Indicators and Reagents; D008242:Lysogeny; D009154:Mutation; D010758:Phosphorus / Q000032:analysis; D011108:Polymers / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D013053:Spectrophotometry; D013210:Staphylococcus / Q000032:analysis* / Q000276:immunology; D013682:Teichoic Acids / Q000032:analysis; D014574:Uronic Acids / Q000032:analysis
chemical_list: D000606:Amino Sugars; D000942:Antigens, Bacterial; D006595:Hexosamines; D007106:Immune Sera; D007202:Indicators and Reagents; D011108:Polymers; D011135:Polysaccharides, Bacterial; D013682:Teichoic Acids; D014574:Uronic Acids; D010758:Phosphorus; D005944:Glucosamine