title: Studies on tryptophan synthetases from various strains of Bacillus subtilis.
abstract: 1. Tryptophan synthetase B of three strains of Bacillus subtilis was prepared from ;exo-protoplastic' and ;endo-protoplastic' fractions; the enzyme from ;exo-protoplastic' fraction was purified 30- to 120-fold by ammonium sulphate precipitation and DEAE-cellulose column chromatography; the latter step separated this enzyme from tryptophan synthetase A, tryptophanase and proteolytic enzymes, but the purified preparations were not stable. 2. The activity of tryptophan synthetase B did not depend on the presence of tryptophan synthetase A. 3. Tryptophan synthetases B of the strains tested differed in their utilization of 2- and 7-methylindole as compared with indole; this suggests that these tryptophan synthetases B are not identical.
mesh_terms: D001412:Bacillus subtilis / Q000166:cytology / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D002482:Cellulose; D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002621:Chemistry; D002852:Chromatography, Ion Exchange; D003864:Depression, Chemical; D004355:Drug Stability; D006836:Hydro-Lyases / Q000302:isolation & purification; D007211:Indoles / Q000494:pharmacology; D008190:Lyases; D008566:Membranes / Q000378:metabolism; D008722:Methods; D010447:Peptide Hydrolases; D011523:Protoplasts / Q000201:enzymology; D013045:Species Specificity; D013268:Stimulation, Chemical; D014170:Transformation, Genetic; D014364:Tryptophan
chemical_list: D007211:Indoles; D014364:Tryptophan; D002482:Cellulose; D010447:Peptide Hydrolases; D008190:Lyases; D006836:Hydro-Lyases