Effect of the method of agitation on the accumulation of protective antigen in cultures of Bacillus anthracis.
Accumulation of protective antigen was essentially independent of culture volume, provided the organisms were kept in suspension by stirring. Agitation by orbited shaking was unsatisfactory.
D000693:Anaerobiosis; D000941:Antigens / Q000378:metabolism*; D000942:Antigens, Bacterial / Q000378:metabolism; D001408:Bacillus anthracis / Q000254:growth & development / Q000276:immunology*; D001431:Bacteriological Techniques; D003168:Complement Fixation Tests; D003470:Culture Media; D005779:Immunodiffusion
D000941:Antigens; D000942:Antigens, Bacterial; D003470:Culture Media