Carbonic acid production and the role of carbonic anhydrase in decarboxylation in brain.
Sudden oxygenation of a thin film of rat brain homogenate, suspended between the surface of a glass pH-sensitive electrode and a gas-permeable membrane, is accompanied by a fall in pH, which is greater when carbonic anhydrase is inhibited. The result suggests that oxidative decarboxylation yields carbonic acid (HCO(3)- and H(+)), which dissociates to form molecular carbon dioxide. Brain carbonic anhydrase facilitates the formation of carbon dioxide from the decarboxylation products.
D000086:Acetazolamide / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism*; D002256:Carbonic Anhydrases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D010084:Oxidation-Reduction; D010100:Oxygen; D051381:Rats
D002245:Carbon Dioxide; D002256:Carbonic Anhydrases; D000086:Acetazolamide; D010100:Oxygen