Deoxyribonucleic acid polymerase activity associated with a plasma particulate fraction from patients with chronic lymphocytic leukemia.
Plasma from two cases of chronic lymphocytic leukemia have been examined for deoxyribonucleic acid (DNA) polymerase activity. In both cases, detectable enzyme activity was present. In the plasma from a patient known to have chronic lymphocytic leukemia for 10 years, the enzyme activity was sufficiently high to study product formation, buoyant density of the enzyme activity, deoxyribonucleoside triphosphate requirements, and kinetics of DNA synthesis. These studies are presented in this report.
D000368:Aged; D001803:Blood Transfusion; D002499:Centrifugation, Density Gradient; D003036:Cobalt Isotopes / Q000627:therapeutic use; D004254:DNA Nucleotidyltransferases / Q000097:blood*; D004269:DNA, Bacterial; D004273:DNA, Neoplasm / Q000096:biosynthesis; D003854:Deoxyribonucleotides / Q000378:metabolism; D004492:Edetic Acid; D005260:Female; D005990:Glycerol; D006801:Humans; D007945:Leukemia, Lymphoid / Q000201:enzymology* / Q000378:metabolism / Q000532:radiotherapy / Q000628:therapy; D008297:Male; D008837:Micrococcus; D010949:Plasma / Q000201:enzymology; D013501:Surface-Active Agents; D013698:Templates, Genetic; D014316:Tritium
D003036:Cobalt Isotopes; D004269:DNA, Bacterial; D004273:DNA, Neoplasm; D003854:Deoxyribonucleotides; D013501:Surface-Active Agents; D014316:Tritium; D004492:Edetic Acid; D004254:DNA Nucleotidyltransferases; D005990:Glycerol