title: Nuclear magnetic resonance studies of the structure and binding sites of enzymes. XI. Characterization of selectively deuterated analogs of staphylococcal nuclease.
abstract: The preparation of five different selectively deuterated analogs of the enzyme staphylococcal nuclease has been previously reported.(1-3) The five selectively deuterated enzymes have full enzymatic activity with DNA and RNA substrates and identical amino acid compositions; and they all appear to have similar conformations in solution, despite their very different hydrogen/deuterium contents.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001665:Binding Sites; D003851:Deoxyribonucleases / Q000032:analysis*; D003903:Deuterium; D004492:Edetic Acid; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D012260:Ribonucleases / Q000032:analysis*; D012996:Solutions; D013210:Staphylococcus / Q000201:enzymology
chemical_list: D000596:Amino Acids; D012996:Solutions; D004492:Edetic Acid; D003903:Deuterium; D003851:Deoxyribonucleases; D012260:Ribonucleases