Transport of aromatic amino acids by Pseudomonas aeruginosa.
Kinetic studies of the transport of aromatic amino acids by Pseudomonas aeruginosa revealed the existence of two high-affinity transport systems which recognized the three aromatic amino acids. From competition data and studies on the exchange of preformed aromatic amino acid pools, the first transport system was found to be functional with phenylalanine, tyrosine, and tryptophan (in order of decreasing activity), whereas the second system was active with tryptophan, phenylalanine, and tyrosine. The two systems also transported a number of aromatic amino acid analogues but not other amino acids. Mutants defective in each of the two and in both transport systems were isolated and described. When the amino acids were added at low external concentrations to cells growing logarithmically in glucose minimal medium, the tryptophan pool very quickly became saturated. Under identical conditions, phenylalanine and tyrosine each accumulated in the intracellular pool of P. aeruginosa at a concentration which was 10 times greater than that of tryptophan.
D000596:Amino Acids / Q000494:pharmacology; D001345:Autoradiography; D001692:Biological Transport / Q000187:drug effects; D002247:Carbon Isotopes; D002855:Chromatography, Thin Layer; D003470:Culture Media; D005827:Genetics, Microbial; D005947:Glucose; D009154:Mutation; D010649:Phenylalanine / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000378:metabolism*; D014364:Tryptophan / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism*
D000596:Amino Acids; D002247:Carbon Isotopes; D003470:Culture Media; D014443:Tyrosine; D010649:Phenylalanine; D014364:Tryptophan; D005947:Glucose