title: Removal of Nonidet P40 absorbance for optical density monitoring of cytoplasmic ribonucleic acid.
abstract: Absorbance at 260 nm due to Nonidet P40 in cytoplasmic extracts can effectively be reduced by extraction with CHCl(3) without appreciable loss of ribonucleic acid.
mesh_terms: D000818:Animals; D002247:Carbon Isotopes; D002460:Cell Line; D002499:Centrifugation, Density Gradient / Q000592:standards*; D002725:Chloroform; D046508:Culture Techniques; D003593:Cytoplasm; D003902:Detergents; D000882:Haplorhini; D007668:Kidney; D012313:RNA / Q000032:analysis*; D012964:Sodium; D012997:Solvents; D013395:Sucrose; D013431:Sulfates; D014529:Uridine
chemical_list: D002247:Carbon Isotopes; D003902:Detergents; D012997:Solvents; D013431:Sulfates; D013395:Sucrose; D012313:RNA; D002725:Chloroform; D012964:Sodium; D014529:Uridine