Paper chromatographic techniques for the determination of cephalothin and desacetylcephalothin in body fluids.
Paper chromatographic procedures may be used to detect cephalothin and its metabolite desacetylcephalothin in urine, plasma, synovial fluid, and cerebrospinal fluid. Protein-bound antibiotics are released from plasma, cerebrospinal fluid, and synovial fluid by dilution with an equal volume of dimethylformamide. Data are presented on the sample preparation, paper chromatographic system, and other specific techniques.
D001412:Bacillus subtilis; D001826:Body Fluids / Q000032:analysis*; D002247:Carbon Isotopes; D002512:Cephalothin / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid / Q000652:urine; D002854:Chromatography, Paper; D004126:Dimethylformamide; D006801:Humans; D008722:Methods; D013170:Spores; D013582:Synovial Fluid / Q000032:analysis
D002247:Carbon Isotopes; D004126:Dimethylformamide; D002512:Cephalothin