Rapid method for the isolation of Listeria monocytogenes from experimentally infected mice.
Listeria monocytogenes was successfully isolated from experimentally infected mice by placing homogenized tissues in phosphate-buffered saline (PBS), trypsin, peptone, or pepsin followed by incubation at 37 C for 24 hr. A larger number of Listeria isolates were recovered from the trypsin or PBS splenic homogenate suspensions incubated at 37 C for 2 hr than from the other diluent suspensions. Holding infected tissues at 4 C for at least 3 months did not increase the efficiency of Listeria isolation. Listeria L-forms were not isolated from mice injected with the bacterial form. The in vitro viability of Listeria L-forms suspended in PBS or PBS splenic homogenate was greatly reduced when held at 4 C.
D000362:Agar; D000818:Animals; D001431:Bacteriological Techniques; D002021:Buffers; D003080:Cold Temperature; D003470:Culture Media; D046508:Culture Techniques; D005704:Gallbladder / Q000382:microbiology; D006863:Hydrogen-Ion Concentration; D007421:Intestine, Small / Q000382:microbiology; D007668:Kidney / Q000382:microbiology; D007740:L Forms; D008089:Listeria monocytogenes / Q000302:isolation & purification*; D008088:Listeriosis / Q000382:microbiology* / Q000473:pathology; D008099:Liver / Q000382:microbiology; D051379:Mice; D010434:Pepsin A; D010461:Peptones; D010710:Phosphates; D012965:Sodium Chloride; D013154:Spleen / Q000382:microbiology; D013997:Time Factors; D014357:Trypsin
D002021:Buffers; D003470:Culture Media; D010461:Peptones; D010710:Phosphates; D012965:Sodium Chloride; D000362:Agar; D014357:Trypsin; D010434:Pepsin A