A specific complex between a restriction endonuclease and its DNA substrate.
In the presence of Mg(++), ATP, and S-adenosylmethionine, the DNA restriction endonuclease R.K forms a specific complex with its DNA substrate. The complex can be detected by its retention on nitrocellulose membranes.
D000255:Adenosine Triphosphate; D002482:Cellulose; D055598:Chemical Phenomena; D002621:Chemistry; D003090:Coliphages; D004279:DNA, Viral; D003851:Deoxyribonucleases; D004492:Edetic Acid; D004926:Escherichia coli / Q000201:enzymology; D007700:Kinetics; D008274:Magnesium; D008567:Membranes, Artificial; D008715:Methionine; D009566:Nitrates; D009705:Nucleosides
D004279:DNA, Viral; D008567:Membranes, Artificial; D009566:Nitrates; D009705:Nucleosides; D000255:Adenosine Triphosphate; D002482:Cellulose; D004492:Edetic Acid; D008715:Methionine; D003851:Deoxyribonucleases; D008274:Magnesium