Epidermal growth factor: high and low molecular weight forms.
In crude homogenates of the submaxillary gland of the male mouse, epidermal growth factor (EGF) activity was found almost entirely in a high molecular weight complex (about 74,000 daltons). This high molecular weight form may be reversibly dissociated into EGF and an EGF-binding protein. EGF was found to have a molecular weight of approximately 6400. The EGF-binding protein has a molecular weight of 29,000, and shows arginyl-esterase activity.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001120:Arginine; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004589:Electrophoresis, Disc; D004950:Esterases; D006133:Growth Substances / Q000302:isolation & purification*; D007106:Immune Sera; D005779:Immunodiffusion; D007525:Isoelectric Focusing; D008297:Male; D051379:Mice; D008970:Molecular Weight; D010455:Peptides / Q000302:isolation & purification*; D011485:Protein Binding; D011817:Rabbits; D013363:Submandibular Gland; D014461:Ultracentrifugation
D000596:Amino Acids; D006133:Growth Substances; D007106:Immune Sera; D010455:Peptides; D001120:Arginine; D004950:Esterases