Ultrasonic synergistic effects in liquid-phase chemical sterilization.
New methods of sterilization employing a chemical with moderate heat and ultrasonic energy have been devised. Inactivation of high-density bacterial spore suspensions is achieved by treatment with low concentration aqueous acid glutaraldehyde solutions at temperatures above or about 54 C. Low (20 kHz) or high (250 kHz) frequency ultrasonic energy is synergistic with glutaraldehyde. Rapid inactivation may also be achieved by using ultrasonic energy and aqueous alkalinized glutaraldehyde solutions at low (25 C) or moderate (55 C) temperatures. If compared to present room temperature techniques, "surface sterilization" time for contaminated objects can be reduced from hours to minutes.
D000447:Aldehydes / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001412:Bacillus subtilis; D001431:Bacteriological Techniques; D002470:Cell Survival; D003470:Culture Media; D004352:Drug Resistance, Microbial; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008722:Methods; D013170:Spores / Q000187:drug effects*; D013171:Spores, Bacterial / Q000187:drug effects / Q000254:growth & development; D013242:Sterilization; D013997:Time Factors; D014465:Ultrasonics / Q000295:instrumentation
D000447:Aldehydes; D000900:Anti-Bacterial Agents; D003470:Culture Media