Relation of mitochondrial phospholipase A activity to mitochondrial swelling.
Assays of mitochondrial phospholipase A activity and mitochondrial swelling demonstrated that the phospholipase A activity is related to the swelling under the experimental conditions used. Both were stimulated by added free fatty acid and CaCl(2), not affected greatly by the addition of monoacyl phosphoglycerides, and inhibited by EDTA. The amount of fatty acid hydrolyzed from endogenous phosphatidyl ethanolamine and phosphatidyl choline during swelling was calculated to be 20-30 times less than the amount of added free fatty acid that gave comparable swelling. Under the experimental conditions about 4% of the phospholipid was hydrolyzed. Mitochondrial swelling was studied by electron microscopy and turbidity measurements. The results found were in agreement, whether oleic acid was present or not, except for those values obtained after very brief incubation (1 min) and after incubation for longer than 35 min. The lack of direct proportion between swelling and the concentration of lysosomes present indicated that the swelling is related mainly to mitochondrial phospholipase A, although swelling due to contaminating lysosomes cannot be excluded entirely. The temperature dependence of spontaneous, fatty acid-induced, or CaCl(2)-induced swelling suggested that enzymatic activities are responsible for swelling.
D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology; D002247:Carbon Isotopes; D002417:Cattle; D003720:Densitometry; D003864:Depression, Chemical; D004492:Edetic Acid / Q000494:pharmacology; D005994:Glycerophosphates / Q000494:pharmacology; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D008247:Lysosomes; D008854:Microscopy, Electron; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D009829:Oleic Acids / Q000494:pharmacology; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism*; D010743:Phospholipids / Q000096:biosynthesis; D051381:Rats; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013268:Stimulation, Chemical; D013696:Temperature; D013997:Time Factors
D002247:Carbon Isotopes; D005994:Glycerophosphates; D009829:Oleic Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D012710:Serum Albumin, Bovine; D004492:Edetic Acid; D010740:Phospholipases; D002122:Calcium Chloride