High-yield method for dispersing simian kidneys for cell cultures.
A technique for dispersion of animal tissue cells is described. The proposed technique is based on the concomitant use of trypsin and disodium ethylenediamine tetraacetate (EDTA). The use of the two dispersing agents (trypsin and disodium EDTA) markedly enhances cell yield as compared with the standard cell dispersion methods. Moreover, significant reduction in the amount of time required for complete tissue dispersal, presence of a very low number of nonviable cells, less cell clumping, and more uniform monolayer formation upon cultivation compare favorably with the results usually obtained with the standard trypsinization technique.
D000818:Animals; D002452:Cell Count; D006224:Cricetinae; D003470:Culture Media; D046508:Culture Techniques; D004492:Edetic Acid; D000882:Haplorhini; D007668:Kidney; D008722:Methods; D051379:Mice; D011817:Rabbits; D051381:Rats; D014020:Tissue Extracts; D014357:Trypsin
D003470:Culture Media; D014020:Tissue Extracts; D004492:Edetic Acid; D014357:Trypsin