Decay of normal and 5-fluorouracil-substituted messenger ribonucleic acid of alkaline phosphatase in Escherichia coli.
The decay of alkaline phosphatase messenger ribonucleic acid (mRNA) in Escherichia coli was studied in cells sensitized to actinomycin D by ethylenediaminetetra-acetate treatment. It was found that in the wild-type strain (K-10) as well as in a nonsense mutant (S26C200), which is phenotypically reversible by treatment with 5-fluorouracil, mRNA decays with a half-life of 2.0 to 2.5 min. Similarly, in a 3-min pulse of labeled 5-fluorouracil, 70% of the incorporated analogue is contained in the labile RNA fraction decomposing with a half-life of 1.8 min.
D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D003609:Dactinomycin / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D005472:Fluorouracil / Q000378:metabolism; D009154:Mutation; D012329:RNA, Bacterial / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D014316:Tritium
D012329:RNA, Bacterial; D012333:RNA, Messenger; D014316:Tritium; D003609:Dactinomycin; D004492:Edetic Acid; D000469:Alkaline Phosphatase; D005472:Fluorouracil