Thiostrepton, an inhibitor of 5OS ribosome subunit function.
Thiostrepton inhibits (14)C-leucine incorporation by intact cells of Bacillus megaterium as well as (14)C-phenylalanine incorporation by a poly U-directed extract of Escherichia coli. Extracts of E. coli which are pretreated by incubation with thiostrepton cannot be reactivated by dialysis to more than 5% of their former activity. The 50S ribosome subunit appears to be the site of thiostrepton action, since protein-synthesizing activity can be restored to dialyzed pretreated extracts by supplementation with 50S ribosome subunits but not with 30S ribosome subunits. This technique also provides a simple sensitive method for detection of the biological activity of very small amounts of 50S ribosome subunits.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001410:Bacillus megaterium / Q000187:drug effects / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis; D002247:Carbon Isotopes; D003864:Depression, Chemical; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology; D010649:Phenylalanine / Q000378:metabolism; D012270:Ribosomes / Q000187:drug effects* / Q000502:physiology; D013936:Thymidine / Q000378:metabolism; D014498:Uracil / Q000378:metabolism
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002247:Carbon Isotopes; D010455:Peptides; D010649:Phenylalanine; D014498:Uracil; D007930:Leucine; D013936:Thymidine