title: Heterogeneity of phospholipid composition in the bacterial membrane.
abstract: Heterogeneity in the distribution or binding of the membrane phospholipids was demonstrated in the membrane fragments released from Haemophilus parainfluenzae by treatment with ethylenediaminetetraacetic acid (EDTA)-tris(hydroxymethyl)-aminomethane (Tris). The membrane fragments released early in the EDTA-Tris treatment contained two- to fivefold higher proportions of cardiolipin and phosphatidylglycerol and less phosphatidylethanolamine as well as phospholipids with threefold lower specific activity of the phospholipid phosphate after a short pulse of (32)P than were found in the residue. Heterogeneity was best demonstrated with shorter EDTA-Tris treatments and shorter periods of growth with (32)P. EDTA-Tris treatment appeared to progressively strip phospholipids from the cells that were synthesized at progressively later times.
mesh_terms: D001345:Autoradiography; D002021:Buffers; D002247:Carbon Isotopes; D002462:Cell Membrane / Q000032:analysis* / Q000378:metabolism; D002855:Chromatography, Thin Layer; D003470:Culture Media; D004492:Edetic Acid / Q000494:pharmacology; D005990:Glycerol / Q000378:metabolism; D006190:Haemophilus / Q000032:analysis* / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis* / Q000378:metabolism; D010759:Phosphorus Isotopes; D012694:Serine / Q000378:metabolism
chemical_list: D002021:Buffers; D002247:Carbon Isotopes; D003470:Culture Media; D010710:Phosphates; D010743:Phospholipids; D010759:Phosphorus Isotopes; D012694:Serine; D004492:Edetic Acid; D005990:Glycerol