Immunoglobulin G and free kapa-chain synthesis in different clones of a hybrid cell line.
13 clonal cell lines were isolated from a hybrid cell population established by cell fusion between cloned BALB/c myeloma cells that were resistant to 8-azaguanine and produced immunoglobulin G and free kappa chain and C(67)BL/6n lymphoma cells that were resistant to bromodeoxyuridine and did not produce immunoglobulins. Some of the histocompatibility-2 antigens of both parental cell lines could be demonstrated on all of the hybrid clones. 11 clones synthesized only free kappa chain. Two clones synthesized IgG of BALB/c type, as well as free kappa chain. These clones had higher chromosome number than did the clones that synthesized only kappa chains.
D000818:Animals; D000941:Antigens; D001375:Azaguanine; D001973:Bromodeoxyuridine; D002459:Cell Fusion; D002460:Cell Line / Q000378:metabolism*; D002999:Clone Cells / Q000378:metabolism*; D003165:Complement System Proteins; D003470:Culture Media; D006648:Histocompatibility; D006822:Hybrid Cells / Q000378:metabolism*; D007106:Immune Sera; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000096:biosynthesis; D007136:Immunoglobulins / Q000096:biosynthesis*; D007621:Karyotyping; D008223:Lymphoma; D051379:Mice; D009101:Multiple Myeloma; D011817:Rabbits; D013045:Species Specificity
D000941:Antigens; D003470:Culture Media; D007106:Immune Sera; D007074:Immunoglobulin G; D007136:Immunoglobulins; D003165:Complement System Proteins; D001973:Bromodeoxyuridine; D001375:Azaguanine