Cataract produced by tyrosinase and tyrosine systems in rabbitens in vitro.
1. Cataracts were developed by incubating rabbit lenses for 22hr. at 37 degrees in a culture medium containing tyrosine and tyrosinase (EC 1.10.3.1). 2. A 45% diminution in the content of GSH and significant inhibition of glucose 6-phosphate dehydrogenase (EC 1.1.1.49) activity were observed in the cataractous lenses compared with controls. 3. GSSG accumulated in both cataractous and control lenses. Significant amounts of GSSG were transported outward from the cataractous lenses and small amounts from control lenses. 4. Transport of GSSG from rabbit lens incubated in a diffusate of plasma from a naphthalene-fed rabbit was also observed. 5. GSSG was found in the aqueous humour obtained between 2 and 24hr. after feeding of naphthalene to rabbits; subsequently the GSSG in the aqueous humour decreased to almost undetectable amounts in 48hr.; in controls, GSSG was not detectable. 6. A possible mechanism of formation of experimental and senile cataract is briefly discussed.
D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism; D001692:Biological Transport; D002386:Cataract / Q000139:chemically induced / Q000209:etiology*; D004156:Catechol Oxidase / Q000494:pharmacology*; D003459:Crystallins / Q000378:metabolism; D003864:Depression, Chemical; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007908:Lens, Crystalline / Q000033:anatomy & histology / Q000187:drug effects*; D009281:Naphthalenes / Q000378:metabolism; D010084:Oxidation-Reduction; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism; D011817:Rabbits; D013438:Sulfhydryl Compounds / Q000032:analysis; D013997:Time Factors; D014443:Tyrosine / Q000494:pharmacology*
D003459:Crystallins; D009281:Naphthalenes; D013438:Sulfhydryl Compounds; D014443:Tyrosine; D007770:L-Lactate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D004156:Catechol Oxidase; D005978:Glutathione