Interaction of concanavalin A with an arabinogalactan from the cell wall of Mycobacterium bovis.
The arabinogalactan isolated from the cell wall of Mycobacterium bovis reacts to form a precipitate with concanavalin A, the phytohemagglutinin of the jack bean. A typical precipitin-like curve is obtained when increasing amounts of the polysaccharide are added to a constant quantity of concanavalin A. Inhibitor studies suggest that concanavalin A reacts with the arabinogalactan by interacting with the C-2, C-3, and C-5 hydroxyl groups of the alpha-d-arabinofuranosyl residues situated at the chain ends of this polysaccharide.
D002473:Cell Wall / Q000032:analysis*; D011232:Chemical Precipitation; D006027:Glycosides / Q000037:antagonists & inhibitors; D005779:Immunodiffusion; D037102:Lectins; D009163:Mycobacterium bovis / Q000032:analysis*; D011134:Polysaccharides / Q000302:isolation & purification*
D006027:Glycosides; D037102:Lectins; D011134:Polysaccharides