title: Phospholipid metabolism in the absence of net phospholipid synthesis in a glycerol-requiring mutant of Bacillus subtilis.
abstract: A glycerol-requiring auxotroph of Bacillus subtilis showed no net synthesis of phospholipid when deprived of glycerol. Although there was no net synthesis of phospholipid, we found that: (i) fatty acids and (32)P were slowly incorporated into phospholipid; (ii) in pulse-chase experiments, both (32)P and (14)C in the glycerol portion of the phospholipids were lost from phosphatidlyglycerol (PG) and lysylphosphatidylglycerol and accumulated in cardiolipin (CL); (iii) the proportions of the phospholipids in the membrane changed with a loss of PG and an accumulation of CL. The addition of glycerol to the glycerol-deprived cells resulted in a rapid incorporation of glycerol and restoration to the predeprivation metabolism and PG to CL ratio.
mesh_terms: D001345:Autoradiography; D001412:Bacillus subtilis / Q000032:analysis / Q000254:growth & development / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D003470:Culture Media; D005227:Fatty Acids / Q000378:metabolism; D005374:Filtration; D005827:Genetics, Microbial; D005990:Glycerol / Q000378:metabolism*; D008055:Lipids / Q000032:analysis / Q000302:isolation & purification; D009154:Mutation; D010743:Phospholipids / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism; D010759:Phosphorus Isotopes
chemical_list: D003470:Culture Media; D005227:Fatty Acids; D008055:Lipids; D010743:Phospholipids; D010759:Phosphorus Isotopes; D005990:Glycerol