The intracellular localization of glycollate oxidoreductase in Euglena gracilis.
1. Lowering of the concentration of carbon dioxide in air available to phototrophically growing Euglena cultures from 5% to the normal value (0.03%) resulted in an increased specific activity of glycollate oxidoreductase. 2. The effects of chloramphenicol and cycloheximide suggested that this increase in activity was due to enzyme synthesis de novo on cytoplasmic ribosomes. 3. The K(m) for glycollate oxidation by the enzyme in crude cell extracts was 3.0x10(-3)m. 4. Differential centrifugation established that glycollate oxidoreductase present in phototrophically grown Euglena is a particulate enzyme. The enzyme was partially solubilized by the non-ionic detergent Triton X-100. 5. Sucrose-density-gradient centrifugation achieved the separation of the particulate glycollate oxidoreductase from chloroplasts and mitochondria. 6. Glutamate-glyoxylate aminotransferase was associated with particulate glycollate oxidoreductase.
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D002245:Carbon Dioxide; D002499:Centrifugation, Density Gradient; D002701:Chloramphenicol / Q000494:pharmacology; D002736:Chloroplasts / Q000201:enzymology; D003513:Cycloheximide / Q000494:pharmacology; D005056:Euglena gracilis / Q000201:enzymology*; D006016:Glycolates / Q000378:metabolism; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology; D012270:Ribosomes / Q000201:enzymology; D013395:Sucrose; D013501:Surface-Active Agents; D000637:Transaminases / Q000378:metabolism
D006016:Glycolates; D013501:Surface-Active Agents; D002245:Carbon Dioxide; D013395:Sucrose; D002701:Chloramphenicol; D003513:Cycloheximide; D000429:Alcohol Oxidoreductases; D000637:Transaminases