Effective protection of monkeys against death from street virus by post-exposure administration of tissue-culture rabies vaccine.
Three series of experiments on rabies vaccines were carried out on rhesus monkeys using suckling-mouse-brain vaccine, rabbit-brain vaccine, duck-embryo vaccine, and purified, concentrated tissue-culture vaccine. The latter was prepared in a human diploid cell strain and inactivated with beta-propiolactone, and consisted of tissue-culture fluid concentrated 200-fold with a final infectivity titre of 10(9.8) plaque-forming units per ml before inactivation. In the first two series of experiments, several vaccines were tested for relative immunogenicity on a pre-exposure basis. In the third series, a successful model was developed in which a single inoculation of the tissue-culture vaccine administered after exposure to rabies virus, with or without accompanying standard doses of antirabies serum, was evaluated as a method of prevention. A single dose of the tissue-culture vaccine protected 7 out of 8 monkeys from death by street virus. Homologous or heterologous antirabies serum alone gave poor results. The results indicate great promise for prophylaxis in man with one dose, or perhaps a few doses, of highly concentrated, purified tissue-culture vaccine.
D000818:Animals; D001921:Brain; D002460:Cell Line; D006224:Cricetinae; D046508:Culture Techniques; D004372:Ducks; D004622:Embryo, Mammalian; D004625:Embryo, Nonmammalian; D000882:Haplorhini; D006801:Humans; D007668:Kidney; D008251:Macaca; D051379:Mice; D008954:Models, Biological; D011818:Rabies / Q000276:immunology*; D011819:Rabies Vaccines; D013997:Time Factors; D014611:Vaccination; D014776:Virus Cultivation
D011819:Rabies Vaccines