Pharmacology of bovine pulmonary vein anaphylaxis in vitro.
1. The bovine pulmonary vein contracts in response to acetylcholine, histamine, 5-hydroxytryptamine and bradykinin. The tissue is particularly sensitive to 5-hydroxytryptamine (>0.1 ng/ml). Specific Schultz-Dale reactions were elicited in the pulmonary vein in response to horse plasma.2. The Schultz-Dale reaction is inhibited both by antihistaminics and by the anti-5-hydroxytryptamine agent methysergide, but not by atropine.3. Sodium meclofenamate inhibited anaphylactic contraction but showed a strong tendency to antagonize many agonists indiscriminately.4. Disodium cromoglycate (DSCG: 100 mug/ml) which inhibits some immunological reactions of mast cells, and diethylcarbamazine citrate (DECC: 50 mug/ml) which inhibits slow-reacting substance of anaphylaxis (SRS-A) elaboration, each inhibited anaphylaxis incompletely (50% or less). However, a combination of DSCG and DECC virtually abolished the Schultz-Dale reaction in this preparation. It is tentatively suggested that the component of the anaphylactic contraction which is resistant to cromoglycate but sensitive to diethylcarbamazine could be due to SRS-A.5. The bovine pulmonary Schultz-Dale reaction appears to be a complex interaction of histamine, 5-hydroxytryptamine, SRS-A and possible other agents including kinins.
D000109:Acetylcholine / Q000494:pharmacology; D000707:Anaphylaxis / Q000188:drug therapy; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001920:Bradykinin / Q000494:pharmacology; D002417:Cattle; D004205:Cromolyn Sodium / Q000494:pharmacology; D004049:Diethylcarbamazine / Q000494:pharmacology; D006632:Histamine / Q000494:pharmacology; D006736:Horses; D066298:In Vitro Techniques; D008297:Male; D008407:Mast Cells / Q000276:immunology; D008784:Methysergide / Q000494:pharmacology; D009369:Neoplasms / Q000175:diagnosis; D011667:Pulmonary Veins / Q000187:drug effects* / Q000276:immunology; D012698:Serologic Tests; D012701:Serotonin / Q000494:pharmacology
D012701:Serotonin; D001285:Atropine; D006632:Histamine; D000109:Acetylcholine; D004205:Cromolyn Sodium; D001920:Bradykinin; D004049:Diethylcarbamazine; D008784:Methysergide