Simplified procedure for preparation of specific antibodies to gamma globulins.
Antibodies were prepared to rhesus monkey, rabbit, dog, and hamster gamma globulins which had been purified by agar-block electrophoresis. Immunoelectrophoretic analysis demonstrated that these antisera were specific for gamma globulin components in whole serum. The use of these antisera as secondary serological reagents was examined.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis / Q000302:isolation & purification*; D000918:Antibody Specificity; D011232:Chemical Precipitation; D006224:Cricetinae; D004285:Dogs; D004586:Electrophoresis; D005455:Fluorescent Antibody Technique; D000882:Haplorhini; D007120:Immunochemistry; D007122:Immunoelectrophoresis; D007202:Indicators and Reagents; D000644:Quaternary Ammonium Compounds; D011817:Rabbits; D013431:Sulfates
D000888:Antibodies, Anti-Idiotypic; D007202:Indicators and Reagents; D000644:Quaternary Ammonium Compounds; D013431:Sulfates