title: "Transcriptional mapping". I. Introduction to the method and the use of actinomycin D as a transcriptional mapping agent.
abstract: The transcription process can in theory be used for genetic mapping on the "intraoperonal" level. We have shown that such an approach, utilizing actinomycin D to create blocks to the travel of the transcription enzyme, is indeed feasible, and the effects of actinomycin D on the synthesis of general pulse-labeled RNA are reported herein.
mesh_terms: D001412:Bacillus subtilis; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003609:Dactinomycin / Q000494:pharmacology*; D004586:Electrophoresis; D005815:Genetic Code; D008722:Methods; D008954:Models, Biological; D008970:Molecular Weight; D009876:Operon; D012329:RNA, Bacterial
chemical_list: D012329:RNA, Bacterial; D003609:Dactinomycin