Uptake of synthetic polynucleotides by competent cells of Bacillus subtilis.
A survey was made of the capacity of competent cells of Bacillus subtilis to take up synthetic polynucleotides. Polydeoxyribonucleotides but not polyribonucleotides are taken up by the cells. Both types of polynucleotide failed to compete with transforming deoxyribonucleic acid in the transformation assay whereas both stimulated amino acid incorporation. The latter phenomenon appears to be nonspecific as there is no correlation between the codon composition of the polynucleotides employed and the amino acids whose incorporation was stimulated.
D000596:Amino Acids / Q000378:metabolism; D001412:Bacillus subtilis / Q000254:growth & development / Q000378:metabolism*; D001431:Bacteriological Techniques; D002247:Carbon Isotopes; D003470:Culture Media; D004269:DNA, Bacterial / Q000378:metabolism; D005827:Genetics, Microbial; D011119:Polynucleotides / Q000378:metabolism* / Q000494:pharmacology; D013997:Time Factors; D014170:Transformation, Genetic / Q000187:drug effects; D014316:Tritium
D000596:Amino Acids; D002247:Carbon Isotopes; D003470:Culture Media; D004269:DNA, Bacterial; D011119:Polynucleotides; D014316:Tritium