Relative affinity of vancomycin and ristocetin for cell walls and uridine diphosphate-N-acetylmuramyl pentapeptide.
A determination of the relative affinity of vancomycin and ristocetin for isolated cell walls and for a peptidoglycan precursor was made. These antibiotics had previously been shown to adsorb to cell walls and to complex with peptides containing a d-alanyl-d-alanine C-terminus. By using (14)C-uridine diphosphate (UDP)-N-acetylmuramyl pentapeptide, it was shown that the complex which is formed between this peptidoglycan precursor and either vancomycin or ristocetin does not preclude adsorption of the antibiotics to cell walls of Micrococcus lysodeikticus. Complex formation between ristocetin and UDP-N-acetylmuramyl pentapeptide was assured by differential absorption spectra. However, when the complex was mixed with cell walls, the antibiotic was sedimented with the walls, and the radioactivity remained in the supernatant solution. This indication that ristocetin and vancomycin have a greater affinity for walls than for UDP-N-acetylmuramyl pentapeptide and that the complex per se does not bind to cell walls suggests that adsorption of these antibiotics to cell walls is probably responsible for the inhibition of peptidoglycan synthesis. This proposal is strengthened by the observation that complexed antibiotic is no less inhibitory for growth of Bacillus subtilis than free vancomycin or ristocetin.
D000327:Adsorption; D001412:Bacillus subtilis / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D002247:Carbon Isotopes; D002473:Cell Wall / Q000378:metabolism; D002854:Chromatography, Paper; D003864:Depression, Chemical; D008837:Micrococcus / Q000187:drug effects / Q000378:metabolism; D010455:Peptides; D012310:Ristocetin / Q000494:pharmacology; D013053:Spectrophotometry; D013210:Staphylococcus / Q000187:drug effects / Q000378:metabolism; D014466:Ultraviolet Rays; D014640:Vancomycin / Q000494:pharmacology
D001426:Bacterial Proteins; D002247:Carbon Isotopes; D010455:Peptides; D012310:Ristocetin; D014640:Vancomycin