Host-virus interaction in ribonucleic acid bacteriophage-infected Escherichia coli. I. Location of "late" MS2-specific ribonucleic acid synthesis.
When actinomycin-treated, MS2-infected Escherichia coli are labeled during a brief period later than 16 min after infection, the newly synthesized MS2 ribonucleic acid (RNA) appears first in the 30,000 x g sediment, probably bound to fragments of bacterial membranes, since the radioactivity can be released from the sediment with deoxycholate or urea. With longer labeling times, radioactivity also appears in the 30,000 x g supernatant fluid. While on the membrane, the RNA is organized into particles with sedimentation coefficients of 40, 32, and 27S in the presence of low Mg(2+). In the presence of high Mg(+), MS2-specific RNA is found in polyribosomes. These data are interpreted to mean that MS2-specific RNA is synthesized and organized into larger structures on membrane. More than 8 min of labeling is required before radioactivity is found in the 81S virion which appears in the supernatant fluid.
D001435:Bacteriophages / Q000378:metabolism*; D001647:Bile Acids and Salts; D001665:Binding Sites; D002247:Carbon Isotopes; D002462:Cell Membrane / Q000032:analysis*; D002498:Centrifugation; D003609:Dactinomycin / Q000494:pharmacology; D004492:Edetic Acid; D004926:Escherichia coli / Q000032:analysis* / Q000187:drug effects; D008274:Magnesium; D012367:RNA, Viral / Q000096:biosynthesis*; D012270:Ribosomes / Q000032:analysis; D013997:Time Factors; D014316:Tritium; D014461:Ultracentrifugation; D014498:Uracil
D001647:Bile Acids and Salts; D002247:Carbon Isotopes; D012367:RNA, Viral; D014316:Tritium; D003609:Dactinomycin; D014498:Uracil; D004492:Edetic Acid; D008274:Magnesium