Effect of the purification of virus antigens on the production of specific complement-fixing antibodies.
The effect of viral purification procedures on the antibody response of guinea pigs to immunization with reovirus type 2 and echovirus type 19 was investigated. Three grades of antigens were employed: (i) infectious monkey kidney tissue culture fluid (TCF), (ii) virus sedimented in the ultracentrifuge and suspended in phosphate-buffered saline, and (iii) virus purified by centrifugation in CsCl density gradients. The antibody response of the guinea pigs was studied by the hemagglutination inhibition, complement fixation, and serum neutralization tests. Only sera produced from virus purified by CsCl density gradients reacted specifically with homologous antigen in the complement fixation test. Sera from animals receiving tissue culture fluid virus or sedimented virus cross-reacted with heterologous antigens such as tissue culture fluid from uninfected monkey kidney cells. All sera, however, reacted specifically in hemagglutination inhibition and serum neutralization tests. Sera from intranasally infected animals (reovirus type 2), even though reacting specifically in the complement fixation test, had much lower titers than sera from animals inoculated intramuscularly.
D000818:Animals; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000302:isolation & purification*; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D003168:Complement Fixation Tests; D003429:Cross Reactions; D046508:Culture Techniques; D003588:Cytopathogenic Effect, Viral; D029822:Enterovirus B, Human / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology; D006168:Guinea Pigs; D000882:Haplorhini; D006385:Hemagglutination Inhibition Tests; D006389:Hemagglutinins, Viral / Q000032:analysis; D006801:Humans; D007106:Immune Sera; D007114:Immunization; D007668:Kidney; D009500:Neutralization Tests; D012087:Reoviridae / Q000276:immunology*; D014461:Ultracentrifugation; D014776:Virus Cultivation
D000941:Antigens; D002712:Chlorides; D006389:Hemagglutinins, Viral; D007106:Immune Sera; D002586:Cesium