Density gradient centrifugation studies on lymphocytic choriomeningitis virus and on viral ribonucleic acid.
Lymphocytic choriomeningitis (LCM) virus, Traub strain, was purified from BHK-21 tissue culture medium. The virus was then analyzed by equilibrium centrifugation and rate zonal centrifugation in sucrose gradients. A buoyant density in sucrose of 1.18 g/ml was found and the S(20, w) value was estimated to be about 470 to 500S. Furthermore, the (3)H-uridine-labeled ribonucleic acid (RNA) from virus was extracted from LCM virus and analyzed by rate zonal centrifugation. Two major and one minor single-stranded RNA components were found with sedimentation coefficients of 28, 22, and 18S.
D000818:Animals; D002247:Carbon Isotopes; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002501:Centrifugation, Zonal; D006224:Cricetinae; D007668:Kidney; D008217:Lymphocytic choriomeningitis virus; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D012367:RNA, Viral / Q000096:biosynthesis / Q000302:isolation & purification*; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D014713:Viruses, Unclassified
D002247:Carbon Isotopes; D012367:RNA, Viral; D014316:Tritium; D012313:RNA; D014529:Uridine