A template-selective inhibitor of in vitro transcription.
A macromolecular factor, TF1, has been isolated from bacteriophage SP01-infected B. subtilis. TF1 selectively inhibits in vitro transcription of SP01 and related viral DNA. Evidence is presented regarding these properties of the repressor-like TF1: (1) template and conformational specificity; (2) interaction with DNA rather than RNA polymerase; (3) trypsin sensitivity; (4) reversibility of action; and (5) ability to block initiation, but not propagation, of RNA synthesis.
D001412:Bacillus subtilis; D001435:Bacteriophages / Q000032:analysis; D004279:DNA, Viral / Q000378:metabolism*; D066298:In Vitro Techniques; D046911:Macromolecular Substances / Q000302:isolation & purification* / Q000494:pharmacology; D012316:RNA Nucleotidyltransferases / Q000378:metabolism*; D012367:RNA, Viral / Q000096:biosynthesis*; D013698:Templates, Genetic; D014357:Trypsin
D004279:DNA, Viral; D046911:Macromolecular Substances; D012367:RNA, Viral; D012316:RNA Nucleotidyltransferases; D014357:Trypsin