Lipid composition of Chlamydia psittaci grown in monkey kidney cells in defined medium.
The lipid compositions of (i) monkey kidney (MK-2) cells cultivated in Eagle's minimal essential medium (MEM) with 5% calf serum, (ii) MK-2 cells cultivated in Waymouth medium supplemented with 20 mug of sodium oleate and 2 mg of bovine albumin per ml, (iii) Chlamydia psittaci strain 6BC grown in the latter host system, and (iv) calf serum were compared. Strain 6BC contains 31% phosphatidyl ethanolamine (PE) and 15% phosphatidyl glycerol (PG), whereas the host cell contains almost the same amount of PE (27%) and no PG. A high concentration of total lipid was observed in strain 6BC (29 to 34%), whereas MK-2 cells contain only 9 to 15% and calf serum contains 4.5% total lipid. The fatty acids of the total lipid from strain 6BC contain branched-chain acids. These fatty acids were found mostly in PE (33.0%) and PG (37.0%). No branched-chain fatty acid was found in the MK-2 cells. There was an increase in triglyceride content when MK-2 cells cultivated in MEM (19.2%) were compared with cells cultivated in Waymouth medium (28.0%). A high concentration (62.0%) of octadecenoic acid (C18:1) was found in the triglyceride of MK-2 cells cultivated in Waymouth medium. The level of polyunsaturated fatty acids observed in MK-2 cells cultivated in Waymouth medium (10.8%) and in the chlamydiae grown in these cells (13.3%) was low compared with the level in MK-2 cells (28.8%) cultivated in MEM with 5% calf serum and the level in calf serum itself (50.8%). A higher ratio of sterol ester to free sterol was found in calf serum than in MK-2 cells or in chlamydiae. Host contribution to lipid composition of strain 6BC is discussed.
D000818:Animals; D002689:Chlamydia / Q000032:analysis*; D002725:Chloroform; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D003470:Culture Media; D046508:Culture Techniques; D005227:Fatty Acids / Q000032:analysis; D005228:Fatty Acids, Essential / Q000032:analysis; D006017:Glycolipids / Q000032:analysis; D000882:Haplorhini; D007668:Kidney / Q000032:analysis*; D008055:Lipids / Q000032:analysis*; D009829:Oleic Acids; D010714:Phosphatidylethanolamines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D012710:Serum Albumin, Bovine / Q000032:analysis*; D012964:Sodium; D013053:Spectrophotometry; D013261:Sterols / Q000032:analysis; D014280:Triglycerides / Q000032:analysis
D003470:Culture Media; D005227:Fatty Acids; D005228:Fatty Acids, Essential; D006017:Glycolipids; D008055:Lipids; D009829:Oleic Acids; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D013261:Sterols; D014280:Triglycerides; D012710:Serum Albumin, Bovine; D002725:Chloroform; D012964:Sodium