title: Comparative immunological studies of two Pseudomonas enzymes.
abstract: Crystalline preparations of muconate lactonizing enzyme and muconolactone isomerase, two inducible enzymes that catalyze successive steps in the catechol branch of the beta-ketoadipate pathway, were used to prepare antisera. Both enzymes were isolated from a strain of Pseudomonas putida biotype A. The antisera did not cross-react with enzymes of the same bacterial strain that catalyze the chemically analogous steps in the protocatechuate branch of the beta-ketoadipate pathway, carboxymuconate lactonizing enzyme and carboxymuconolactone decarboxylase. The antisera gave heterologous cross-reactions of varying intensities with the muconate lactonizing enzymes and muconolactone isomerases of P. putida biotype B, P. aeruginosa, P. stutzeri, and all biotypes of P. fluorescens, but did not cross-react with the isofunctional enzymes of P. acidovorans, of P. multivorans, and of two bacterial species that belong to other genera. The evolutionary and taxonomic implications of the findings are discussed.
mesh_terms: D000818:Animals; D001565:Benzoates; D002262:Carboxy-Lyases; D002396:Catechols / Q000378:metabolism; D003168:Complement Fixation Tests; D003470:Culture Media; D004586:Electrophoresis; D004790:Enzyme Induction; D005782:Gels; D007106:Immune Sera; D005779:Immunodiffusion; D007535:Isomerases; D008297:Male; D011549:Pseudomonas / Q000145:classification / Q000201:enzymology* / Q000276:immunology / Q000378:metabolism; D011817:Rabbits; D013045:Species Specificity; D013053:Spectrophotometry; D013213:Starch; D013386:Succinates
chemical_list: D001565:Benzoates; D002396:Catechols; D003470:Culture Media; D005782:Gels; D007106:Immune Sera; D013386:Succinates; D013213:Starch; D002262:Carboxy-Lyases; D007535:Isomerases