title: Deoxyribonucleic acid replication and expression of early and late bacteriophage functions in Bacillus subtilis.
abstract: The role of deoxyribonucleic acid (DNA) replication in the control of the synthesis of deoxycytidylate (dCMP) deaminase and lysozyme in Bacillus subtilis infected with bacteriophage 2C has been studied. These phage-induced enzymes are synthesized at different times during the latent period. It was shown by actinomycin inhibition that the formation of the late enzyme (lysozyme) required messenger ribonucleic acid (mRNA) synthesized de novo after the initiation of translation of mRNA which specifies the early function (dCMP deaminase). The inhibition of phage DNA synthesis by mitomycin C prevented the synthesis of lysozyme only when added before the onset of phage DNA replication, but it did not affect the synthesis or action of dCMP deaminase when added at any time during the latent period. Treatment of infected cells with mitomycin C after phage DNA synthesis had reached 8 to 10% of its maximal rate resulted in the production of normal amounts of lysozyme. These observations suggest that mRNA specifying early enzymes can be transcribed from parental (and probably also from progeny) DNA, whereas late functional messengers can be transcribed only after the formation of progeny DNA.
mesh_terms: D000619:Aminohydrolases / Q000096:biosynthesis*; D001412:Bacillus subtilis / Q000276:immunology*; D001435:Bacteriophages / Q000302:isolation & purification; D003597:Cytosine Nucleotides / Q000096:biosynthesis; D004261:DNA Replication; D004279:DNA, Viral; D003609:Dactinomycin / Q000494:pharmacology; D008937:Mitomycins / Q000494:pharmacology; D009113:Muramidase / Q000032:analysis*; D012367:RNA, Viral / Q000096:biosynthesis; D013941:Thymine; D013997:Time Factors; D014316:Tritium; D014529:Uridine
chemical_list: D003597:Cytosine Nucleotides; D004279:DNA, Viral; D008937:Mitomycins; D012367:RNA, Viral; D014316:Tritium; D003609:Dactinomycin; D009113:Muramidase; D000619:Aminohydrolases; D013941:Thymine; D014529:Uridine