title: Purification of intact flagella from Escherichia coli and Bacillus subtilis.
abstract: A procedure is described for the purification of bacterial flagella in the form of a filament-hook-basal body complex (intact flagella) free from detectable cell wall, membrane, or cytoplasmic material. Spheroplasts produced with lysozyme and ethylenediaminetetraacetic acid were lysed with Triton X-100, and the flagella were purified by (NH(4))(2)SO(4) precipitation, differential centrifugation, and CsCl gradient centrifugation. As much as 40% of the flagella were recovered, and they contained about one basal body per 4 to 6 mum of flagella. The same procedure developed for Escherichia coli was also successful for purifying intact flagella from Bacillus subtilis.
mesh_terms: D001412:Bacillus subtilis / Q000032:analysis / Q000166:cytology* / Q000254:growth & development; D001426:Bacterial Proteins / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D003902:Detergents; D003956:Dialysis; D000644:Quaternary Ammonium Compounds
chemical_list: D001426:Bacterial Proteins; D002241:Carbohydrates; D002712:Chlorides; D003902:Detergents; D000644:Quaternary Ammonium Compounds; D002586:Cesium