In vitro excision-repair of ultraviolet-irradiated transforming DNA from Bacillus subtilis.
The biological activity of UV-inactivated Bacillus subtilis DNA is partly restored after incubation with a UV-specific endonuclease from Micrococcus lutens in conjunction with DNA polymerase and DNA ligase, both isolated from Escherichia coli. The restored activity is not further increased by photoreactivation. Pyrimidine dimers are specifically liberated when irradiated DNA is exposed to the three enzymes. None of these effects is observed when pancreatic DNase is used instead of UV-specific endonuclease.
D001412:Bacillus subtilis / Q000201:enzymology / Q000378:metabolism*; D002478:Cells, Cultured; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D004260:DNA Repair; D004269:DNA, Bacterial / Q000528:radiation effects*; D003624:Darkness; D003851:Deoxyribonucleases / Q000378:metabolism; D004789:Enzyme Activation; D004926:Escherichia coli / Q000201:enzymology; D008025:Ligases / Q000378:metabolism; D008837:Micrococcus / Q000201:enzymology; D010179:Pancreas / Q000201:enzymology; D010777:Photochemistry; D011108:Polymers / Q000378:metabolism; D011743:Pyrimidines / Q000378:metabolism; D011830:Radiation Effects; D014170:Transformation, Genetic; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D011108:Polymers; D011743:Pyrimidines; D004254:DNA Nucleotidyltransferases; D003851:Deoxyribonucleases; D008025:Ligases