Complement-fixing antigen from BHK-21 cell cultures infected with lymphocytic choriomeningitis virus.
Infection of BHK-21 cells with lymphocytic choriomeningitis (LCM) virus resulted in the production of significant titers of complement-fixing (CF) antigen. The antigen was spontaneously released from the cells, but the highest titer of 1:16 was recovered by disruption of the infected cells by freeze-thawing in tryptose phosphate broth. The antigen could be partially separated from infectious virus by centrifugation. Furthermore, it was possible to detect LCM virus infection of cell cultures by the production of the CF antigen, but this method proved less sensitive than titration by intracerebral inoculation of mice. The CF antigen from cell cultures was at least as sensitive and specific as the reference antigen prepared from infected guinea pig spleen.
D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000032:analysis; D001681:Biological Assay; D002460:Cell Line; D002498:Centrifugation; D003168:Complement Fixation Tests; D006224:Cricetinae; D046508:Culture Techniques; D005615:Freezing; D006168:Guinea Pigs; D006358:Hot Temperature; D006801:Humans; D007668:Kidney; D008216:Lymphocytic Choriomeningitis / Q000382:microbiology*; D008217:Lymphocytic choriomeningitis virus; D051379:Mice; D013154:Spleen / Q000276:immunology; D014713:Viruses, Unclassified
D000941:Antigens