title: LPP-1 infection of the blue-green alga Plectonema boryanum. II. Viral deoxyribonucleic acid synthesis and host deoxyribonucleic acid breakdown.
abstract: Host and viral deoxyribonucleic acid (DNA) metabolism in LPP-1-infected Plectonema boryanum was studied by equilibrium centrifugation in CsCl gradients. Approximately 50% of the host DNA is degraded to acid-soluble material between 3 and 7 hr after infection. Most of the acid-soluble product is reincorporated into viral DNA. Incorporation of exogenous (3)H-adenine into viral DNA can be detected very early after infection (within the first 2 hr), but the bulk of viral DNA synthesis occurs between 6 and 8 hr. Both the breakdown of host DNA and the synthesis of viral DNA require protein synthesis during the first few hours of infection.
mesh_terms: D000225:Adenine / Q000378:metabolism; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002701:Chloramphenicol / Q000494:pharmacology; D002712:Chlorides; D000458:Cyanobacteria / Q000187:drug effects / Q000378:metabolism*; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism*; D004279:DNA, Viral / Q000096:biosynthesis* / Q000302:isolation & purification; D010942:Plant Viruses / Q000378:metabolism / Q000472:pathogenicity*; D014176:Protein Biosynthesis; D013997:Time Factors; D014316:Tritium
chemical_list: D002247:Carbon Isotopes; D002712:Chlorides; D004279:DNA, Viral; D014316:Tritium; D002586:Cesium; D002701:Chloramphenicol; D004247:DNA; D000225:Adenine