Effect of host cell on distribution of a lysosomal enzyme during virus infection.
The time of appearance of a lysosomal enzyme, beta-glucuronidase, in the medium of cells infected with either measles virus or echovirus 6 varied with the host cell system. Replication and release of virus preceded leakage of beta-glucuronidase from green monkey kidney cells. In contrast, extracellular enzyme appeared before replication and release of virus in human amnion cells. Hydrocortisone depressed enzyme leakage but did not retard replication of measles virus or viral-induced cytopathology. The intracellular distribution of beta-glucuronidase in uninfected and measles virus-infected cells was also studied. Measles virus infection altered the position of particulate-bound beta-glucuronidase in linear sucrose gradients prior to substantial release of this enzyme intra- and extracellularly. At early stages in infection, most of the cell-associated virus banded with particulate-bound enzyme in the middle of the gradient. As infection progressed, separation of measles virus infectivity from enzyme activity occurred, and intracellular virus was recovered near the meniscus of sucrose gradients.
D000650:Amnion; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured / Q000201:enzymology; D002499:Centrifugation, Density Gradient; D003470:Culture Media; D029822:Enterovirus B, Human / Q000254:growth & development / Q000472:pathogenicity*; D005966:Glucuronidase / Q000378:metabolism*; D000882:Haplorhini; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D007668:Kidney; D008247:Lysosomes / Q000201:enzymology*; D008459:Measles virus / Q000254:growth & development / Q000472:pathogenicity*; D013395:Sucrose; D013997:Time Factors; D014779:Virus Replication
D003470:Culture Media; D013395:Sucrose; D005966:Glucuronidase; D006854:Hydrocortisone