Chloroplast RNA populations in dark-grown, light-grown, and greening Euglena gracilis.
RNA preparations from dark-grown, light-grown, and greening Euglena gracilis have been compared by polyacrylamide gel electrophoresis and by hybridization to Euglena chloroplast DNA. Chloroplast ribosomal RNA is not detected in dark-grown cells; its abundance increases in greening cells over a 72 hr period until the concentration characteristic of light-grown cells is reached. Other RNA species complementary to chloroplast DNA are present in comparable abundance in light-grown, dark-grown, and greening cells.
D001345:Autoradiography; D002736:Chloroplasts / Q000032:analysis*; D003593:Cytoplasm / Q000032:analysis; D004247:DNA / Q000032:analysis; D003624:Darkness; D004589:Electrophoresis, Disc; D005056:Euglena gracilis / Q000032:analysis* / Q000254:growth & development; D008027:Light; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010759:Phosphorus Isotopes; D012313:RNA / Q000032:analysis*; D012335:RNA, Ribosomal / Q000032:analysis; D013053:Spectrophotometry
D010759:Phosphorus Isotopes; D012335:RNA, Ribosomal; D012313:RNA; D004247:DNA