Large-scale isolation and fractionation of organs of Drosophila melanogaster larvae.
Methods for the mass isolation of diverse organs from small animals are described. They involve novel devices: a mechanical dissecting system, a centrifugal agitator for the separation of fibrillar from globular particles, and a settling chamber for the fractionation at unit gravity of particles with sedimentation velocities above the useful range for centrifugation. The application of these methods to the isolation of polytene and nonpolytene nuclei from Drosophila melanogaster larvae is described.
D000818:Animals; D003585:Cell Biology / Q000295:instrumentation*; D002458:Cell Fractionation / Q000295:instrumentation; D002467:Cell Nucleus; D002498:Centrifugation / Q000295:instrumentation; D002499:Centrifugation, Density Gradient; D004210:Dissection / Q000295:instrumentation; D004331:Drosophila melanogaster / Q000166:cytology*; D007814:Larva / Q000166:cytology; D008297:Male; D008317:Malpighian Tubules / Q000166:cytology; D008722:Methods; D008858:Microscopy, Phase-Contrast; D012469:Salivary Glands / Q000166:cytology; D013737:Testis / Q000166:cytology