Early energy-dependent step in the entry of transforming deoxyribonucleic acid.
It was found that cyanide can partially reverse as well as arrest the transformation of a newly transformed deoxyribonuclease-treated culture for 6 to 8 min after the addition of the enzyme. These findings strongly suggest that deoxyribonucleic acid which attained deoxyribonuclease-insensitivity is not necessarily in the cell, but stored in some region peripheral to the membrane, and that a rate-limiting, energy-dependent step is required to transport this deoxyribonucleic acid into the cell.
D001412:Bacillus subtilis / Q000187:drug effects*; D002463:Cell Membrane Permeability; D003080:Cold Temperature; D003470:Culture Media; D003486:Cyanides / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000378:metabolism* / Q000494:pharmacology; D003851:Deoxyribonucleases / Q000494:pharmacology; D004735:Energy Transfer; D013997:Time Factors
D003470:Culture Media; D003486:Cyanides; D004269:DNA, Bacterial; D003851:Deoxyribonucleases