LPP-1 infection of the blue-green alga Plectonema boryanum. 3. Protein synthesis.
The structural proteins of mature LPP-1 particles and the patterns of protein synthesis after LPP-1 infection have been examined by electrophoresis on sodium dodecyl sulfate polyacrylamide gels. Structural proteins account for 35% of the LPP-1 genome, and proteins that would require about 65% of the total coding capacity have been detected after infection. The major head proteins have molecular weights of 39,000 and 13,000, whereas the major tail protein is an 80,000-molecular-weight species. Host protein synthesis is depressed soon after infection and appears to be entirely shut off by 5 hr. Three classes of viral proteins are distinguished in infected cells, based on their time course of synthesis and their presence in mature virions.
D000179:Acrylates; D001345:Autoradiography; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002701:Chloramphenicol / Q000494:pharmacology; D002712:Chlorides; D000458:Cyanobacteria / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D003720:Densitometry; D004586:Electrophoresis; D005782:Gels; D008970:Molecular Weight; D010942:Plant Viruses / Q000032:analysis / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism / Q000472:pathogenicity* / Q000528:radiation effects; D014176:Protein Biosynthesis; D012964:Sodium; D013431:Sulfates; D013460:Sulfur Isotopes; D013997:Time Factors; D014466:Ultraviolet Rays; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis*
D000179:Acrylates; D002712:Chlorides; D005782:Gels; D013431:Sulfates; D013460:Sulfur Isotopes; D014764:Viral Proteins; D002586:Cesium; D002701:Chloramphenicol; D012964:Sodium