Lipoprotein inhibitor of Newcastle disease virus from chicken lung.
A lipoprotein inhibitor of Newcastle disease virus was obtained from chicken lung tissue by means of dilute alkaline extraction procedures. The inhibitor was further purified by ammonium sulfate fractionation, isoelectric precipitation, and density gradient centrifugation. The purified lipoprotein inhibited active Newcastle disease virus hemagglutination at a concentration of 2.0 mug/ml which represented a 30-fold purification over the original extract. Infection of chicken embryo fibroblasts by Newcastle disease virus was also inhibited by the purified lipoprotein, the degree of inhibition depending upon the inhibitor-to-virus ratio. Chemical analysis of the purified inhibitor provided a composition of 72% lipid, 26% protein, and 3% carbohydrate, although some compositional variation was observed from one preparation to another. The chloroform-soluble lipids were shown to contain 40 to 50% phospholipid and 10 to 20% cholesterol; of the fatty acids recovered from the saponified lipoprotein, 39% was palmitic, 22% oleic, and 17% stearic. Careful analyses of large quantities of the inhibitor revealed a small (0.84%) but significant content of sialic acid. Removal of sialic acid from the lipoprotein by means of digestion with neuraminidase produced a sharp diminution in inhibitory properties. A delipidized form of the inhibitor was obtained by ether extraction, and this material produced a single broad band of precipitate in gel immunodiffusion tests.
D000818:Animals; D001095:Arachidonic Acids / Q000302:isolation & purification; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D002645:Chickens; D002784:Cholesterol / Q000302:isolation & purification; D005347:Fibroblasts / Q000187:drug effects; D006387:Hemagglutination, Viral; D005779:Immunodiffusion; D008041:Linoleic Acids / Q000302:isolation & purification; D008074:Lipoproteins / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D008168:Lung / Q000382:microbiology; D009438:Neuraminic Acids / Q000302:isolation & purification; D009439:Neuraminidase; D009522:Newcastle disease virus / Q000187:drug effects*; D009829:Oleic Acids / Q000302:isolation & purification; D010169:Palmitic Acids / Q000302:isolation & purification; D010743:Phospholipids / Q000302:isolation & purification; D013229:Stearic Acids / Q000302:isolation & purification
D001095:Arachidonic Acids; D008041:Linoleic Acids; D008074:Lipoproteins; D009438:Neuraminic Acids; D009829:Oleic Acids; D010169:Palmitic Acids; D010743:Phospholipids; D013229:Stearic Acids; D002784:Cholesterol; D009439:Neuraminidase