Standardized viral hemagglutination and hemagglutination-inhibition tests. II. Description and statistical evaluation.
Standardized hemagglutination and hemagglutination-inhibition procedures are described and statistically evaluated for all animal viruses where applicable, except for rubella and the arbovirus group. The standardized tests employ a constant phosphate-buffered saline diluent and constant volumes of serum, antigen, and standardized erythrocyte suspension. The standardized hemagglutination test has a reproducibility of 84 to 96% with adenoviruses, rubeola, and the myxoviruses, and 78 to 93% with reoviruses; the standardized hemagglutination-inhibition test has a reproducibility of 95 to 100% with all viruses tested.
D000256:Adenoviridae / Q000276:immunology; D000941:Antigens; D002021:Buffers; D004912:Erythrocytes; D006385:Hemagglutination Inhibition Tests / Q000592:standards*; D006386:Hemagglutination Tests / Q000592:standards*; D007106:Immune Sera; D009975:Orthomyxoviridae / Q000276:immunology; D012412:Rubella virus / Q000276:immunology; D013223:Statistics as Topic; D014780:Viruses / Q000276:immunology*
D000941:Antigens; D002021:Buffers; D007106:Immune Sera