Simultaneous use of contrasting fluorochromes to separate measles and canine distemper viruses in a common system.
Measles and canine distemper viruses were grown together in a Vero monkey kidney cell line. Each virus could be identified and individually titrated by using the color contrast produced by the reddish tetra-methyl rhodamine isothiocyanate-tagged antimeasles conjugate and the green fluorescein isothiocyanate-tagged antidistemper conjugate. Both blue light and green light were used for the excitation of the fluorochromes. Incident light was transmitted to the specimen by a vertical illuminator of the Ploem type.
D000818:Animals; D002460:Cell Line; D004217:Distemper Virus, Canine / Q000254:growth & development / Q000302:isolation & purification*; D004285:Dogs; D005456:Fluorescent Dyes; D000882:Haplorhini; D006736:Horses; D007106:Immune Sera; D007668:Kidney; D008027:Light; D008459:Measles virus / Q000254:growth & development / Q000302:isolation & purification*; D008722:Methods; D008856:Microscopy, Fluorescence; D010784:Photomicrography; D014765:Viral Vaccines; D014776:Virus Cultivation
D005456:Fluorescent Dyes; D007106:Immune Sera; D014765:Viral Vaccines