Staphylococcal enterotoxin B: solid-phase radioimmunoassay.
An immunoassay employing (125)I-labeled enterotoxin B and polystyrene tubes coated with specific antibody was used for assaying purified and crude enterotoxin. Antibody was adsorbed to untreated polystyrene tubes. Unlabeled enterotoxin competed with (125)I-labeled enterotoxin for antibody-combining sites. The uptake of (125)I-labeled toxin reflected the concentration of unlabeled toxin present. The test is sensitive to 1 to 5 ng of purified and crude enterotoxin B per ml, and cross-reactions with heterologous enterotoxins did not interfere with the specificity. This test possesses the combination of sensitivity and objectivity absent in current methods for assaying enterotoxin and provides a model for investigating other enterotoxin serotypes.
D000818:Animals; D000937:Antigen-Antibody Reactions; D003429:Cross Reactions; D004768:Enterotoxins / Q000032:analysis*; D005504:Food Analysis; D007106:Immune Sera; D005779:Immunodiffusion; D007456:Iodine Isotopes; D008433:Mathematics; D008722:Methods; D011137:Polystyrenes; D011485:Protein Binding; D011817:Rabbits; D011863:Radioimmunoassay; D013210:Staphylococcus
D004768:Enterotoxins; D007106:Immune Sera; D007456:Iodine Isotopes; D011137:Polystyrenes