title: Regulation of the mandelate pathway in Pseudomonas aeruginosa.
abstract: The pathway of mandelate metabolism in Pseudomonas aeruginosa is composed of the following steps: l(+)-mandelate --> benzoylformate --> benzaldehyde --> benzoate. These three steps are unique to mandelate oxidation; the benzoate formed is further metabolized via the beta-ketoadipate pathway. The first enzyme, l(+)-mandelate dehydrogenase, is induced by its substrate. The second and third enzymes, benzoylformate decarboxylase and benzaldehyde dehydrogenase, are both induced by benzoylformate. The same benzaldehyde dehydrogenase, or one very similar to it, is also induced by beta-ketoadipate, an intermediate in the subsequent metabolism of benzoate. This dehydrogenase may also be induced by adipate or a metabolite of adipate. These conclusions have been drawn from the physiological and genetic properties of wild-type P. aeruginosa strains and from the study of mutants lacking the second and third enzyme activities.
mesh_terms: D000272:Adipates / Q000096:biosynthesis / Q000378:metabolism; D000693:Anaerobiosis; D001565:Benzoates / Q000096:biosynthesis / Q000378:metabolism; D002262:Carboxy-Lyases / Q000378:metabolism*; D002396:Catechols / Q000096:biosynthesis; D002474:Cell-Free System; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003470:Culture Media; D004589:Electrophoresis, Disc; D004790:Enzyme Induction; D005827:Genetics, Microbial; D005947:Glucose / Q000378:metabolism; D006358:Hot Temperature; D007651:Keto Acids / Q000096:biosynthesis; D007773:Lactates / Q000378:metabolism; D008333:Mandelic Acids / Q000378:metabolism*; D009153:Mutagens; D009154:Mutation; D009604:Nitrosoguanidines; D010088:Oxidoreductases / Q000378:metabolism*; D010105:Oxygenases / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D013053:Spectrophotometry; D014161:Transduction, Genetic
chemical_list: D000272:Adipates; D001565:Benzoates; D002396:Catechols; D003470:Culture Media; D007651:Keto Acids; D007773:Lactates; D008333:Mandelic Acids; D009153:Mutagens; D009604:Nitrosoguanidines; D010088:Oxidoreductases; D010105:Oxygenases; D002262:Carboxy-Lyases; D005947:Glucose