Isolation and chromosomal localization of highly repeated DNA sequences in Drosophila melanogaster.
The nuclear DNA of D. melanogaster contains DNA sequences that are repeated between ten and a hundred times more often than the next class of redundant DNA. This DNA, as a renatured duplex isolated on the basis of its renaturation kinetics, has a buoyant density of 1.691 (g/ml). In its native state it bands within the unique nuclear DNA peak (rho = 1.701). These sequences have been localized by "in situ" hybridization in the chromocenter of the chromosomes of the salivary gland. The properties of centromeres are discussed in terms of the occurrence of repeated sequences at this locus.
D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D002499:Centrifugation, Density Gradient; D002623:Chemistry Techniques, Analytical; D002875:Chromosomes / Q000032:analysis*; D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D004331:Drosophila melanogaster; D006570:Heterochromatin / Q000032:analysis; D006358:Hot Temperature; D007700:Kinetics; D008961:Models, Structural; D008970:Molecular Weight; D009587:Nitrogen Isotopes; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009695:Nucleic Acid Renaturation; D012469:Salivary Glands / Q000032:analysis
D006570:Heterochromatin; D009587:Nitrogen Isotopes; D004247:DNA