Requirement for coenzyme A in the phosphoroclastic reaction of anaerobic bacteria.
Various bacteria which degrade pyruvate by the phosphoroclastic reaction were examined with respect to the role of coenzyme A (CoA) in this reaction. The strictly anaerobic bacteria, which cleave pyruvate by the phosphoroclastic reaction characteristic of Clostridia, required catalytic levels of CoA for the CO(2)-pyruvate exchange and acetoin-forming portions of the phosphoroclastic reaction. These reactions were reversibly inhibited by the CoA analogue, desulfo-CoA. In contrast, using cell-free extracts of bacteria which degrade pyruvate by the coliform phosphoroclastic reaction (pyruvate formate-lyase), no requirement for CoA could be observed for the formate-pyruvate exchange reaction. It is suggested that CoA serves a regulatory function in the early portion of the clostridal type of phosphoroclastic reaction.
D000388:Air; D001419:Bacteria / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D003013:Clostridium / Q000378:metabolism*; D003065:Coenzyme A / Q000378:metabolism* / Q000494:pharmacology; D003901:Desulfovibrio / Q000378:metabolism; D013293:Enterococcus faecalis / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D008837:Micrococcus / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism*; D012505:Sarcina / Q000378:metabolism
D010710:Phosphates; D011773:Pyruvates; D002245:Carbon Dioxide; D003065:Coenzyme A