L-Asparaginase from Proteus vulgaris.
To produce an immunologically and enzymologically new type of l-asparaginase, 108 strains of bacteria were screened for enzyme production. As a result, 13 bacteria belonging to the genera Alcaligenes, Bacterium, and Proteus were found to produce l-asparaginases in high levels. Among these l-asparaginases, partially purified l-asparaginases from B. cadaveris and P. vulgaris showed antitumor activity. A partially purified l-asparaginase preparation of P. vulgaris did not react with the antibody of Escherichia colil-asparaginase on the Ouchterlony agar plate. Culture conditions for the production of l-asparaginase by P. vulgaris were investigated in detail. The enzyme was produced in high yields when cells were grown aerobically in a medium containing sodium fumarate and corn steep liquor. The addition of glucose or ammonium ion to the medium, however, resulted in depressed production of l-asparaginase. Under the optimum conditions, 3,700 international units of l-asparaginase was obtained from 1 liter of culture medium.
D000332:Aerobiosis; D000421:Alcaligenes / Q000201:enzymology / Q000276:immunology; D000645:Ammonium Sulfate; D000818:Animals; D001215:Asparaginase / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism / Q000627:therapeutic use; D002474:Cell-Free System; D011232:Chemical Precipitation; D003013:Clostridium / Q000201:enzymology / Q000276:immunology; D003470:Culture Media; D004926:Escherichia coli / Q000201:enzymology / Q000276:immunology; D005260:Female; D005650:Fumarates; D007106:Immune Sera; D005779:Immunodiffusion; D007274:Injections, Intraperitoneal; D051379:Mice; D008815:Mice, Inbred Strains; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000188:drug therapy / Q000401:mortality; D011511:Proteus / Q000201:enzymology* / Q000276:immunology; D011817:Rabbits; D013997:Time Factors; D003313:Zea mays
D003470:Culture Media; D005650:Fumarates; D007106:Immune Sera; D001215:Asparaginase; D000645:Ammonium Sulfate