Incorporation of 5-methyl- and 5-hydroxy-tryptophan into the protein of Bacillus subtilis.
Addition of the tryptophan analogues 5-methyl-tryptophan, 5-hydroxy-tryptophan, and 7-aza-tryptophan, during tryptophan starvation of a tryptophan auxotroph of Bacillus subtilis, stimulated amino acid incorporation. Two of the analogues tested, 5-methyl-tryptophan and 5-hydroxy-tryptophan, competed with each other, but their addition did not prevent tryptophan incorporation into acid-insoluble material. The incorporation of tryptophan and 5-hydroxy-tryptophan was stimulated when a mixture of phenylalanine and tyrosine was present in the medium. The two analogues 5-methyl-tryptophan and 5-hydroxy-tryptophan were recovered in protein hydrolysates of analogue-grown cultures, and there is evidence suggesting that the analogues are not chain terminators. Neither analogue prevented the polysome degradation that occurs during tryptophan starvation.
D000596:Amino Acids / Q000096:biosynthesis* / Q000378:metabolism; D001412:Bacillus subtilis / Q000254:growth & development / Q000378:metabolism*; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D002855:Chromatography, Thin Layer; D003470:Culture Media; D008239:Lysine / Q000378:metabolism; D010649:Phenylalanine / Q000494:pharmacology; D011492:Protein Hydrolysates / Q000032:analysis; D012270:Ribosomes / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D013217:Starvation; D014364:Tryptophan / Q000378:metabolism*; D014443:Tyrosine / Q000494:pharmacology; D014498:Uracil / Q000378:metabolism
D000596:Amino Acids; D002247:Carbon Isotopes; D003470:Culture Media; D011492:Protein Hydrolysates; D012701:Serotonin; D014443:Tyrosine; D010649:Phenylalanine; D014498:Uracil; D014364:Tryptophan; D008239:Lysine