The catabolism of phosphatidylethanolamine by the rumen protozoon Entodinium caudatum and its conversion into the N-(1-carboxyethyl) derivative.
1. The N-(2-hydroxyethyl)alanine esterified to phosphatidic acid in anaerobic ciliate rumen protozoa has the l configuration. 2. Labelling experiments with Entodinium caudatum cultures using [(32)P]P(i) [2-(14)C]ethanolamine and (32)P- and (14)C-labelled phosphatidylethanolamine show that phosphatidylethanolamine is the direct lipid precursor of the N-(2-hydroxyethyl)alanine-containing phospholipid. 3. Labelling experiments with [(14)C]starch, [(14)C]lactate and [(14)C]pyruvate with E. caudatum cultures indicate that a three-carbon glycolytic intermediate is probably the precursor of the N-(1-carboxyethyl) grouping which substitutes on the amino group of phosphatidylethanolamine. 4. [(32)P]phosphatidylethanolamine is catabolized by E. caudatum forming initially glycerylphosphorylethanolamine and subsequently glycerophosphate and P(i). A little phosphorylethanolamine formed may possibly arise from bacterial enzymes ingested by the protozoa.
D000409:Alanine / Q000378:metabolism; D000605:Amino Alcohols / Q000378:metabolism; D000818:Animals; D002247:Carbon Isotopes; D016798:Ciliophora / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D010759:Phosphorus Isotopes; D011773:Pyruvates / Q000378:metabolism; D012417:Rumen / Q000382:microbiology*; D013213:Starch / Q000378:metabolism
D000605:Amino Alcohols; D002247:Carbon Isotopes; D007773:Lactates; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D010759:Phosphorus Isotopes; D011773:Pyruvates; D013213:Starch; D000409:Alanine