Detection of a rapidly metabolizing portion of the membrane cardiolipin in Haemophilus parainfluenzae.
Heterogeneity in the metabolism of cardiolipin (CL) has been detected in Haemophilus parainfluenzae. Pulse-chase experiments showed that a portion of the total CL incorporated and then lost (32)P much more rapidly than the rest of the CL in the cells. The metabolism of each phosphate of the CL differed. The phosphate of the phosphatidyl glycerol (PG) portion of the CL had a more active metabolism than the phosphate of the phosphatidic acid portion of the molecule. Only a portion of the PG pool contributed to the formation of CL. Ethylenediaminetetraacetic acid inhibited the CL-specific phospholipase D in vitro and, when added to growing cells, resulted in more rapid PG metabolism, suggesting that CL hydrolysis contributed to the PG pool.
D001345:Autoradiography; D002473:Cell Wall / Q000032:analysis* / Q000378:metabolism; D002854:Chromatography, Paper; D004492:Edetic Acid / Q000494:pharmacology; D004579:Electron Transport; D006190:Haemophilus / Q000032:analysis / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D006868:Hydrolysis; D008055:Lipids / Q000032:analysis; D010710:Phosphates / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010740:Phospholipases / Q000037:antagonists & inhibitors / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis / Q000378:metabolism*; D010759:Phosphorus Isotopes; D013053:Spectrophotometry
D008055:Lipids; D010710:Phosphates; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D010759:Phosphorus Isotopes; D004492:Edetic Acid; D010740:Phospholipases