title: Evidence for a multiplicity of capsular types among Staphylococcus aureus strains.
abstract: The Smith diffuse variant and the wound mucoid strain of Staphylococcus aureus were shown to exhibit serologically distinct capsules. The Welwood and K-6 strains of S. aureus were tested to determine their capsular types. Both Welwood and K-6 were found to be representative of the Smith capsular type. An additional 13 isolates of S. aureus from mice were tested. Gel double-diffusion tests and immunoelectrophoresis of staphylococcal antigens disclosed the possible existence of at least two additional capsular types. Passive hemagglutination tests carried out with cells sensitized with 1 mg of antigen per ml showed a multiplicity of cross-reacting antigens. However, cells sensitized either with 0.1 or 0.05 mg of antigen per ml and reacted with antisera absorbed with 10 or 1 mug/ml showed the presence of a specific antigen in each strain of S. aureus. Corroborative evidence for a multiplicity of capsular types was obtained by the specific capsular reaction. At least four capsular types of S. aureus were found. The prototypic strains for these antigens are the RLM or wound strain, the Smith diffuse strain, and mouse strains designated 36T and 43R. We propose to designate these types 1, 2, 3, and 4, respectively.
mesh_terms: D000042:Absorption; D000941:Antigens / Q000302:isolation & purification; D006386:Hemagglutination Tests; D007106:Immune Sera; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D013210:Staphylococcus / Q000145:classification* / Q000166:cytology / Q000276:immunology*
chemical_list: D000941:Antigens; D007106:Immune Sera