Selection of cells transformed to prototrophy for sporulation markers.
A method is presented for reproducible quantitative selection of recombinants for spore markers in transformation analysis.
D000085:Acetates; D001412:Bacillus subtilis / Q000254:growth & development / Q000302:isolation & purification*; D002364:Caseins; D002725:Chloroform; D002951:Citrates; D003433:Crosses, Genetic; D003470:Culture Media; D004269:DNA, Bacterial; D005971:Glutamates; D007773:Lactates; D008722:Methods; D009154:Mutation; D011492:Protein Hydrolysates; D011995:Recombination, Genetic; D013170:Spores / Q000254:growth & development*; D014170:Transformation, Genetic
D000085:Acetates; D002364:Caseins; D002951:Citrates; D003470:Culture Media; D004269:DNA, Bacterial; D005971:Glutamates; D007773:Lactates; D011492:Protein Hydrolysates; D002725:Chloroform