Removal of Nonidet P40 absorbance for optical density monitoring of cytoplasmic ribonucleic acid.
Absorbance at 260 nm due to Nonidet P40 in cytoplasmic extracts can effectively be reduced by extraction with CHCl(3) without appreciable loss of ribonucleic acid.
D000818:Animals; D002247:Carbon Isotopes; D002460:Cell Line; D002499:Centrifugation, Density Gradient / Q000592:standards*; D002725:Chloroform; D046508:Culture Techniques; D003593:Cytoplasm; D003902:Detergents; D000882:Haplorhini; D007668:Kidney; D012313:RNA / Q000032:analysis*; D012964:Sodium; D012997:Solvents; D013395:Sucrose; D013431:Sulfates; D014529:Uridine
D002247:Carbon Isotopes; D003902:Detergents; D012997:Solvents; D013431:Sulfates; D013395:Sucrose; D012313:RNA; D002725:Chloroform; D012964:Sodium; D014529:Uridine