title: In vitro and in vivo studies of streptomycin-dependent cholera vibrios.
abstract: Streptomycin-dependent cholera vibrio strains were derived from Inaba, Ogawa, and NAG vibrios by the method of Mel. These phenotypes grew more slowly and attacked fermentable substances after a longer period of time than the streptomycin-sensitive parent strains. Rabbits injected with streptomycin-sensitive strains and their streptomycin-dependent forms showed homologous agglutinin production. Patas monkeys fed with 10(9) streptomycin-dependent strains shed them for 1 to 2 days without ill effect, whereas the same number of streptomycin-independent organisms caused disease. The possibility of the application of multiple doses of streptomycin-dependent organisms in oral immunization against cholera was considered.
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D000906:Antibodies; D001431:Bacteriological Techniques; D050260:Carbohydrate Metabolism; D002771:Cholera / Q000517:prevention & control; D003470:Culture Media; D004352:Drug Resistance, Microbial; D005285:Fermentation; D005827:Genetics, Microbial; D000882:Haplorhini; D006801:Humans; D007106:Immune Sera; D007114:Immunization; D008854:Microscopy, Electron; D009154:Mutation; D010461:Peptones; D010641:Phenotype; D011817:Rabbits; D013307:Streptomycin / Q000378:metabolism*; D014733:Vibrio / Q000032:analysis / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology / Q000378:metabolism / Q000472:pathogenicity
chemical_list: D000906:Antibodies; D003470:Culture Media; D007106:Immune Sera; D010461:Peptones; D013307:Streptomycin