Effect of actinomycin D on the transfer of ribonucleic acid from nucleus to cytoplasm in Lactobacillus acidophilus.
After starvation for deoxyribosides, the deoxyribonucleic acid (DNA) of Lactobacillus acidophilus is restricted to a localized region of the cell. (3)H-uracil is first incorporated into such a restricted region but subsequently is found throughout the cell. This spread occurs despite the absence of protein synthesis and a major reduction in the rate of ribonucleic acid (RNA) synthesis. However, blocking RNA synthesis with actinomycin D restricts incorporation to a localized region of the cell. It is concluded that uracil is first incorporated into RNA in the bacterial nucleus from which it subsequently spreads through the cell. Actinomycin D could prevent this spread by preventing the completion of RNA molecules, which therefore do not dissociate from the DNA template.
D001345:Autoradiography; D001692:Biological Transport / Q000187:drug effects; D002247:Carbon Isotopes; D002467:Cell Nucleus / Q000378:metabolism; D003470:Culture Media; D003593:Cytoplasm / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology*; D007779:Lactobacillus acidophilus / Q000187:drug effects / Q000378:metabolism*; D008854:Microscopy, Electron; D012329:RNA, Bacterial / Q000096:biosynthesis / Q000378:metabolism*; D013941:Thymine / Q000378:metabolism; D014316:Tritium; D014498:Uracil / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D002247:Carbon Isotopes; D003470:Culture Media; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D014316:Tritium; D003609:Dactinomycin; D014498:Uracil; D013941:Thymine; D014529:Uridine