Estimating the number of organisms in quantal assays.
It has been demonstrated by virologists that, when procedural difficulties can be overcome and host variation can be eliminated, dosage-response data from virus assays conform with the one-particle theory of infection for both plaque counts and tissue cultures. Based on this theory, the number of virus particles from quantal virus assays can be estimated. Here a set of tables is presented from which the number of estimated particles can be obtained directly for several dilution factors and a number of dilution levels. Maximum likelihood estimation of particle counts is also illustrated using a computer program that we have prepared.
D000704:Analysis of Variance; D002452:Cell Count; D007928:Lethal Dose 50; D008433:Mathematics; D008954:Models, Biological; D014776:Virus Cultivation; D014780:Viruses