A remeasurement of the molecular weights of T-even bacteriophage substructural proteins.
T-even bacteriophage substructural proteins were studied by using discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis. It was found that tail fibers are composed of two major proteins of 155,000 and 120,000 daltons molecular weight and four minor proteins of 51,000, 38,000, 27,000, and 23,000 daltons. Tail tubes were composed of one predominant protein of 18,500 daltons and one minor protein of 35,000 daltons molecular weight. Tubular polyheads obtained from a T4D amber mutant and by treatment of T4B-infected cells with L-canavanine were also examined, and no significant differences were noted in the molecular weight of the P23 protein.
D002172:Canavanine / Q000494:pharmacology; D002845:Chromatography; D003090:Coliphages / Q000032:analysis*; D004589:Electrophoresis, Disc; D005827:Genetics, Microbial; D008854:Microscopy, Electron; D008970:Molecular Weight; D009154:Mutation; D012967:Sodium Dodecyl Sulfate; D014764:Viral Proteins / Q000032:analysis*
D014764:Viral Proteins; D012967:Sodium Dodecyl Sulfate; D002172:Canavanine