Use of gas chromatography for determining catabolic products of arginine by bacteria.
A rapid and sensitive procedure for determining catabolic products of arginine metabolism by bacteria was developed. The method consists of inoculating a solution of L-arginine with a heavy cell suspension of the test organism. After a 2-hr incubation period, dissimilation products (citrulline, ornithine, agmatine, putrescine) are converted to volatile derivatives and analyzed by gas-liquid chromatography. Compared with conventional microbiological tests, the new procedure is rapid and can be used for sensitive quantitative measurements of specific metabolites from arginine.
D001119:Arginase / Q000378:metabolism; D001120:Arginine / Q000378:metabolism*; D001419:Bacteria / Q000201:enzymology / Q000378:metabolism*; D002172:Canavanine / Q000032:analysis / Q000096:biosynthesis; D002262:Carboxy-Lyases / Q000378:metabolism; D002849:Chromatography, Gas; D002956:Citrulline / Q000032:analysis / Q000096:biosynthesis; D006867:Hydrolases / Q000378:metabolism; D009952:Ornithine / Q000032:analysis / Q000096:biosynthesis; D011073:Polyamines / Q000032:analysis; D011700:Putrescine / Q000032:analysis / Q000096:biosynthesis; D013045:Species Specificity; D013237:Stereoisomerism
D011073:Polyamines; D002956:Citrulline; D002172:Canavanine; D001120:Arginine; D009952:Ornithine; D006867:Hydrolases; D001119:Arginase; D002262:Carboxy-Lyases; D011700:Putrescine