Mass purification of nucleopolyhedrosis virus inclusion bodies in the K-series centrifuge.
Nucleopolyhedrosis virus inclusion bodies specific for Hemerocampa pseudotsugata, Neodiprion sertifer, Porthetria dispar, and Heliothis zea have been purified by using a continuous-sample-flow-with-isopycnic-banding centrifuge in quantities up to 6 x 10(13) polyhedral inclusion bodies per day. Continuous-flow methods for S-rho type purification have been evolved to deal with mass isolation of bioparticles.
D000818:Animals; D002499:Centrifugation, Density Gradient; D007181:Inclusion Bodies, Viral; D007304:Insect Viruses / Q000302:isolation & purification*; D007313:Insecta; D007814:Larva; D008722:Methods; D013395:Sucrose
D013395:Sucrose