Oxalate-silica gel thin-layer system for free 2-hydroxy fatty acids and for fatty acyl coenzyme A.
The 2-hydroxy fatty acids tend to yield streaks in thin-layer chromatography on silica gel plates. If potassium oxalate is included with binder-free silica gel, good spots are obtained. Similar difficulties are found in paper chromatography of the fatty acid derivatives of coenzyme A, especially with long-chain acids. The same thin-layer system gives good spots with these compounds.
D002855:Chromatography, Thin Layer; D003065:Coenzyme A / Q000302:isolation & purification; D005227:Fatty Acids / Q000302:isolation & purification*; D005233:Fatty Alcohols / Q000302:isolation & purification; D008722:Methods; D010070:Oxalates; D011188:Potassium
D005227:Fatty Acids; D005233:Fatty Alcohols; D010070:Oxalates; D011188:Potassium; D003065:Coenzyme A