Radio-immunoassay of gastrin in human plasma.
1. A radio-immunoassay for gastrin has been developed using partially purified porcine gastrin to raise antibodies and highly purified natural porcine gastrin I for radio-iodination with (125)I. The separation of antibody-bound from free hormone was performed by a double-antibody method.2. In this assay highly purified natural porcine gastrin I, synthetic human gastrin I, radio-iodinated porcine gastrin I, gastrin in the plasma of a healthy volunteer, a patient with pernicious anaemia and another patient with the Zollinger-Ellison syndrome were immunologically identical.3. The fasting plasma gastrin concentration of fourteen gastric ulcer patients was significantly higher than that of the 113 hospital controls with no history of gastro-intestinal disease, while twenty-seven duodenal ulcer patients had gastrin levels within the normal range.4. Plasma gastrin concentration was significantly elevated in pernicious anaemia (fifty-one patients), achlorhydria (thirty-three patients), hypochlorhydria (eleven patients) and in nine patients with histologically proven Zollinger-Ellison syndrome.5. In human volunteers a protein meal stimulated endogenous gastrin release while a carbohydrate meal did not. Atropine sulphate I.M., and hydrochloric acid orally, produced a significant fall in the level of circulating gastrin.
D000126:Achlorhydria / Q000097:blood; D000752:Anemia, Pernicious / Q000097:blood; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D004044:Dietary Proteins; D004381:Duodenal Ulcer / Q000097:blood; D005755:Gastrins / Q000097:blood* / Q000378:metabolism; D006168:Guinea Pigs; D006801:Humans; D006851:Hydrochloric Acid / Q000494:pharmacology; D007456:Iodine Isotopes; D008722:Methods; D011817:Rabbits; D011863:Radioimmunoassay; D013268:Stimulation, Chemical; D013276:Stomach Ulcer / Q000097:blood; D013395:Sucrose / Q000494:pharmacology; D013552:Swine; D015043:Zollinger-Ellison Syndrome / Q000097:blood
D004044:Dietary Proteins; D005755:Gastrins; D007456:Iodine Isotopes; D013395:Sucrose; D001285:Atropine; D006851:Hydrochloric Acid