Composition of fatty acids and carbohydrates in Leptospira.
The fatty acid and monosaccharide composition of four pathogenic and two saprophytic strains of Leptospira was analyzed by gas chromatography (GC) and GC-mass spectrometry. Among the fatty acids, palmitic acid was most abundant and constituted 30 to 50% of the total fatty acids. Even-numbered unsaturated acids including octadecenoic, hexadecenoic, octadecadienoic, and tetradecadienoic acids comprised 40 to 60% of the total fatty acids. Tetradecanoic acid was about 5% in saprophytic strains, but 1% or less in pathogenic strains. The amount of chloroform-methanol extract of L. biflexa strain Ancona was 14 to 20% of the dry weight of the cell. Tetradecadienoic acid was found in the chloroform-methanol insoluble fraction, suggesting the presence of the acid in a bound form. GC analysis of monosaccharides revealed the existence of arabinose, xylose, rhamnose, mannose, galactose, glucose, glucosamine, and muramic acid in the cells. Among the neutral sugars, glucose was a minor component and was especially low in pathogenic strains. Total pentose content was about two to three times greater than total hexose.
D000606:Amino Sugars / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis*; D002725:Chloroform; D002849:Chromatography, Gas; D003470:Culture Media; D004952:Esters / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis*; D005228:Fatty Acids, Essential / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D006601:Hexoses / Q000032:analysis; D007919:Leptospira / Q000737:chemistry* / Q000254:growth & development / Q000472:pathogenicity; D007921:Leptospira interrogans / Q000737:chemistry; D041341:Leptospira interrogans serovar canicola / Q000737:chemistry; D008055:Lipids / Q000032:analysis; D013058:Mass Spectrometry; D000432:Methanol; D009005:Monosaccharides / Q000032:analysis; D009696:Nucleic Acids / Q000032:analysis; D010169:Palmitic Acids / Q000032:analysis; D010429:Pentoses / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000032:analysis; D012997:Solvents; D014574:Uronic Acids / Q000032:analysis
D000606:Amino Sugars; D002241:Carbohydrates; D003470:Culture Media; D004952:Esters; D005227:Fatty Acids; D005228:Fatty Acids, Essential; D006601:Hexoses; D008055:Lipids; D009005:Monosaccharides; D009696:Nucleic Acids; D010169:Palmitic Acids; D010429:Pentoses; D011135:Polysaccharides, Bacterial; D012997:Solvents; D014574:Uronic Acids; D002725:Chloroform; D005944:Glucosamine; D000432:Methanol