Preservation of lipid reesterifying enzymes in the microsomal fraction of the rat jejunum.
Studies were performed on methods of storage of rat jejunal tissue that would preserve activities of the lipid reesterifying enzymes, acyl CoA:monoglyceride acyltransferase and fatty acid CoA ligase. Storage at -80 degrees C of microsomes prepared from jejunal mucosa or storage of lyophilized microsomes at -20 degrees C was shown to preserve acyl CoA:monoglyceride acyltransferase very well for a matter of weeks. Preservation of fatty acid CoA ligase activity was adequate with either method, but results were not as good as for the transacylase enzyme.
D000217:Acyltransferases; D000818:Animals; D003065:Coenzyme A; D003080:Cold Temperature; D004355:Drug Stability; D005227:Fatty Acids; D005612:Freeze Drying; D005989:Glycerides; D007413:Intestinal Mucosa / Q000166:cytology / Q000201:enzymology; D007583:Jejunum / Q000166:cytology / Q000201:enzymology*; D008025:Ligases; D008297:Male; D008722:Methods; D008861:Microsomes / Q000201:enzymology; D051381:Rats; D013997:Time Factors
D005227:Fatty Acids; D005989:Glycerides; D000217:Acyltransferases; D008025:Ligases; D003065:Coenzyme A