Transcription of the adenovirus genome by an  -amanitine-sensitive ribonucleic acid polymerase in HeLa cells.
The properties of the ribonucleic acid (RNA) polymerase activity which transcribes the major portion of the adenovirus genome were studied. Nuclei were prepared from infected cells and incubated in vitro. Virus-specific RNA was determined by hybridization to adenovirus deoxyribonucleic acid (DNA). Adenovirus DNA is transcribed principally by an activity which resembles closely polymerase II of the host cell. This activity is inhibited by alpha-amanitine and stimulated by (NH(4))(2)SO(4). Its product is high-molecular-weight heterogeneous RNA. The polymerase activity measured early in infection (3 to 5 hr) resembles that found late in infection (16 to 18 hr).
D000256:Adenoviridae / Q000254:growth & development / Q000378:metabolism*; D000645:Ammonium Sulfate / Q000494:pharmacology; D002247:Carbon Isotopes; D002467:Cell Nucleus / Q000201:enzymology / Q000378:metabolism; D004279:DNA, Viral; D005815:Genetic Code; D005827:Genetics, Microbial; D006367:HeLa Cells / Q000201:enzymology / Q000382:microbiology*; D006801:Humans; D008345:Manganese / Q000494:pharmacology; D008970:Molecular Weight; D009183:Mycotoxins / Q000494:pharmacology*; D009693:Nucleic Acid Hybridization; D012316:RNA Nucleotidyltransferases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012367:RNA, Viral / Q000096:biosynthesis*; D013997:Time Factors; D014316:Tritium; D014500:Uracil Nucleotides / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D002247:Carbon Isotopes; D004279:DNA, Viral; D009183:Mycotoxins; D012367:RNA, Viral; D014500:Uracil Nucleotides; D014316:Tritium; D008345:Manganese; D012316:RNA Nucleotidyltransferases; D000645:Ammonium Sulfate; D014529:Uridine