Studies with cholroplast and mitochondrial DNA. I. Evidence of sequence homology between chloroplast and nuclear DNA (Broad Bean) and between mitochondrial and nuclear DNA (rat liver).
A mixture of broad bean chloroplast and nuclear DNA or rat liver mitochondrial and nuclear DNA was taken through a heating and annealing cycle, and examined by CsCl density gradient centrifugation. The formation of intermediates between the two DNAs during joint annealing was used as a method of detecting sequence homology in different DNA samples. Homology was found between chloroplast and nuclear DNA from broad bean and between mitochondrial and nuclear DNA from rat liver. Since this method produces only qualitative data no implication for possible nucleocytoplasmic relationship can be assessed.
D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000032:analysis*; D002499:Centrifugation, Density Gradient; D002736:Chloroplasts / Q000032:analysis*; D003597:Cytosine Nucleotides / Q000032:analysis; D004247:DNA / Q000032:analysis*; D006150:Guanine Nucleotides / Q000032:analysis; D006358:Hot Temperature; D008099:Liver / Q000032:analysis* / Q000166:cytology; D008930:Mitochondria, Liver / Q000032:analysis*; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009695:Nucleic Acid Renaturation; D059828:Plant Cells; D010944:Plants / Q000032:analysis*; D051381:Rats; D013053:Spectrophotometry; D014466:Ultraviolet Rays
D003597:Cytosine Nucleotides; D006150:Guanine Nucleotides; D004247:DNA