Simple procedure for isolation of satellite DNA's from tobacco leaves in high yield and demonstration of minicircles.
D002467:Cell Nucleus / Q000032:analysis; D002499:Centrifugation, Density Gradient; D002736:Chloroplasts / Q000032:analysis / Q000187:drug effects; D003597:Cytosine Nucleotides / Q000032:analysis; D004247:DNA / Q000032:analysis / Q000302:isolation & purification*; D003851:Deoxyribonucleases / Q000494:pharmacology; D006150:Guanine Nucleotides / Q000032:analysis; D006358:Hot Temperature; D007038:Hypotonic Solutions; D007700:Kinetics; D008854:Microscopy, Electron; D008928:Mitochondria / Q000032:analysis / Q000187:drug effects; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009695:Nucleic Acid Renaturation; D010944:Plants / Q000032:analysis*; D010947:Plants, Toxic; D014026:Nicotiana / Q000032:analysis / Q000166:cytology / Q000187:drug effects
D003597:Cytosine Nucleotides; D006150:Guanine Nucleotides; D007038:Hypotonic Solutions; D004247:DNA; D003851:Deoxyribonucleases