Characterization of temperate actinophage phi C31 isolated from Streptomyces coelicolor A3(2).
Actinophage phiC31 isolated from Streptomyces coelicolor A3(2), the only strain among actinomycetes for which a genetic map had been constructed, appears to be a typical temperate phage. After phiC31 infection, true lysogenic cultures arose which liberated phage and were immune to infection with homologous phage after repeated single-colony isolations and treatment with phage-specific antiserum. Clear-plaque (c) mutants were derived from phiC31 phage which failed to lysogenize sensitive cultures. Actinophage phiC31 has a temperature-sensitive stage of reproduction. A phage which reproduces with the same effectiveness at high (37 C) and low (28 C) temperatures has also been obtained. Heat-inducible (ct) mutants were isolated from this phage which were able to lysogenize sensitive cultures at 28 C but failed to do so at 37 C. Properties of ct mutants suggest that ct mutations involve a gene controlling maintenance of the lysogenic state in actinomycetes and synthesizing repressor, which may become heat-sensitive as a result of mutation.
D001434:Bacteriophage Typing; D001435:Bacteriophages / Q000302:isolation & purification*; D003433:Crosses, Genetic; D005809:Genes, Regulator; D005827:Genetics, Microbial; D006358:Hot Temperature; D007106:Immune Sera; D008242:Lysogeny; D009154:Mutation; D013171:Spores, Bacterial / Q000254:growth & development; D013302:Streptomyces / Q000254:growth & development*; D013696:Temperature; D013997:Time Factors; D014776:Virus Cultivation; D014779:Virus Replication
D007106:Immune Sera