Virus-associated nucleases: evidence for endonuclease and exonuclease activity in rabbitpox and vaccinia viruses.
The endonucleolytic action of a deoxyribonuclease activity in rabbitpox and vaccinia virus was established by change in sedimentation rate of denatured (3)H-lambda deoxyribonucleic acid substrate. The presence of two deoxyribonuclease activities in pox-virus is confirmed. Exo- and endonuclease activities are unmasked by treatment of purified virus with the detergent Nonidet P-40 and further enhanced by treatment of viral "cores" with trypsin.
D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D004279:DNA, Viral; D003851:Deoxyribonucleases / Q000378:metabolism*; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D009691:Nucleic Acid Denaturation; D011212:Poxviridae / Q000201:enzymology*; D013395:Sucrose; D013501:Surface-Active Agents; D014316:Tritium; D014357:Trypsin; D014616:Vaccinia virus / Q000201:enzymology*
D004279:DNA, Viral; D013501:Surface-Active Agents; D014316:Tritium; D013395:Sucrose; D003851:Deoxyribonucleases; D014357:Trypsin