Method for accelerated identification of arboviruses after inoculation of mice.
Sequential titration of infective virus and complement-fixing antigen in brain and liver of suckling mice infected with the following virus strains-Dugbe (a new arbovirus), Congo (related to Crimean hemorrhagic fever virus), yellow fever, dengue 1 and dengue 2-showed a progressive increase in titer after infection. High titers of both infective virus and complement-fixing antigen were demonstrated long before the mice showed clinical signs of infection. It is suggested that earlier isolation and identification of arboviruses from clinical and field specimens can be made if serological tests are done before mice are moribund.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001102:Arbovirus Infections / Q000276:immunology / Q000382:microbiology*; D001103:Arboviruses / Q000276:immunology* / Q000302:isolation & purification*; D001921:Brain / Q000276:immunology / Q000382:microbiology; D003168:Complement Fixation Tests; D003716:Dengue Virus / Q000276:immunology / Q000302:isolation & purification; D007928:Lethal Dose 50; D008099:Liver / Q000276:immunology / Q000382:microbiology; D008722:Methods; D051379:Mice; D013997:Time Factors; D015005:Yellow fever virus / Q000276:immunology / Q000302:isolation & purification
D000956:Antigens, Viral