Biosynthesis of C4 (fourth component of complement) by hybrids of C4-deficient guinea pig cells and HeLa cells.
Peritoneal exudate cells from guinea pigs homozygous for a genetic deficiency of the fourth component of complement (C4) were fused in vitro with a cell line of human origin (HeLa). The resulting hybrid cells, derived from cell lines each incapable of C4 synthesis by themselves, synthesized functionally active human C4.
D000818:Animals; D003165:Complement System Proteins / Q000032:analysis / Q000096:biosynthesis*; D003470:Culture Media; D006168:Guinea Pigs; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells / Q000378:metabolism*; D007153:Immunologic Deficiency Syndromes / Q000235:genetics*; D007621:Karyotyping; D008938:Mitosis; D011863:Radioimmunoassay
D003470:Culture Media; D003165:Complement System Proteins