Mixed function oxidase and ethanol metabolism in perfused rat liver.
Oxidation-reduction changes of cytochrome P-450 and oxygen consumption were measured in isolated perfused livers from normal and phenobarbital-treated rats. Phenobarbital treatment markedly increased the aminopyrine-induced reduction of cytochrome P-450, but ethanol did not cause any redox changes of this cytochrome. It was concluded that the microsomal ethanol-oxidizing system has an insignificant role in the metabolism of ethanol in intact liver.
D000632:Aminopyrine / Q000494:pharmacology; D000818:Animals; D002248:Carbon Monoxide / Q000494:pharmacology; D003580:Cytochromes / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism* / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D010477:Perfusion; D010634:Phenobarbital / Q000008:administration & dosage / Q000494:pharmacology; D010860:Pigments, Biological / Q000378:metabolism; D051381:Rats
D003580:Cytochromes; D010860:Pigments, Biological; D000632:Aminopyrine; D000431:Ethanol; D002248:Carbon Monoxide; D010634:Phenobarbital