Relationship between yield of protoplasts and growth phase in Saccharomyces.
A number of generations must occur in the presence of glucose before daughter cells of stationary or old cultures become amenable to protoplast formation by the action of snail enzyme.
D003470:Culture Media; D000431:Ethanol; D005947:Glucose; D008278:Magnesium Sulfate; D010461:Peptones; D011523:Protoplasts; D012440:Saccharomyces / Q000254:growth & development*; D012908:Snails / Q000201:enzymology; D015003:Yeasts
D003470:Culture Media; D010461:Peptones; D000431:Ethanol; D008278:Magnesium Sulfate; D005947:Glucose