The life-span and recirculation of marrow-derived small lymphocytes from the rat thoracic duct.
These experiments describe the preparation of pure marrow-derived lymphocyte suspensions from the thoracic duct of thymectomized, irradiated rats reconstituted with bone marrow cells. The majority of marrow-derived cells were small lymphocytes morphologically indistinguishable from small lymphocytes in thoracic duct lymph of normal donors. Marrow-derived small lymphocytes (B lymphocytes) were a predominantly long-lived population; the frequency of short-lived B lymphocytes in the thoracic duct was not significantly higher than the frequency of short-lived small lymphocytes in normal lymph. B lymphocytes transferred to normal recipients recirculated from blood to lymph. The first appearance of intravenously injected B lymphocytes in the thoracic duct was delayed relative to lymphocytes from normal donors and there was no clear cut modal recirculation time. Nevertheless their recirculation over a 48 hr period after transfusion was of the same order of magnitude as that of lymphocytes from normal donors.
D000818:Animals; D000921:Antibody-Producing Cells / Q000378:metabolism; D001345:Autoradiography; D001853:Bone Marrow / Q000378:metabolism; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D005260:Female; D008208:Lymphatic System / Q000502:physiology; D008214:Lymphocytes / Q000254:growth & development / Q000502:physiology*; D008221:Lymphoid Tissue / Q000166:cytology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013897:Thoracic Duct / Q000166:cytology; D013934:Thymectomy; D013997:Time Factors; D014316:Tritium; D014529:Uridine
D014316:Tritium; D014529:Uridine