Removal of nonspecific antiproteinases from serum of rabbits hyperimmunized against Micrococcus and Staphylococcus proteinases.
The naturally occurring (nonspecific) proteinase inhibitor which occurs in the alpha globulin fraction of immune rabbit serum was separated from specific staphylococcal and micrococcal antiproteinases by treatment of the antiserum with 2-ethoxy-6-9-diaminacridine. The 2-ethoxy-6-9-diaminacridine-treated serum could then be used to classify these bacterial proteinases by testing directly on casein agar without prior electrophoresis of the serum.
D000166:Acridines; D000362:Agar; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D002364:Caseins; D004586:Electrophoresis; D005612:Freeze Drying; D007106:Immune Sera; D008722:Methods; D008837:Micrococcus / Q000145:classification / Q000201:enzymology / Q000276:immunology*; D011480:Protease Inhibitors; D011817:Rabbits / Q000276:immunology; D013210:Staphylococcus / Q000145:classification / Q000201:enzymology / Q000276:immunology*
D000166:Acridines; D000906:Antibodies; D002364:Caseins; D007106:Immune Sera; D011480:Protease Inhibitors; D000362:Agar