Simple dye release assay for determining cellulolytic activity of fungi.
A colorimetric assay, based on the release of dye from a paper substrate, is described as a simple and rapid means of detecting cellulolytic activity. It is readily adaptable to rapidly screening in situ large numbers of fungal liquid cultures. Since it is a nondestructive assay, samples can be recovered for later use.
D002482:Cellulose / Q000378:metabolism*; D003124:Colorimetry; D004396:Coloring Agents; D005947:Glucose / Q000032:analysis / Q000096:biosynthesis; D006026:Glycoside Hydrolases / Q000032:analysis*; D008722:Methods; D003904:Mitosporic Fungi / Q000201:enzymology* / Q000378:metabolism; D010209:Paper; D012037:Refuse Disposal; D013053:Spectrophotometry; D013997:Time Factors
D004396:Coloring Agents; D002482:Cellulose; D006026:Glycoside Hydrolases; D005947:Glucose