Studies on isolated membranes of azurophil and specific granules from rabbit polymorphonuclear leukocytes.
Membranes were prepared from rabbit polymorphonuclear leukocyte azurophil and specific granules separated by zonal differential centrifugation. The two types of granule membranes were quite similar in ultrastructural appearance, but they showed distinct differences in cholesterol-phospholipid ratios and in protein components demonstrable in polyacrylamide gels.
D000818:Animals; D001202:Ascitic Fluid / Q000032:analysis / Q000166:cytology; D002458:Cell Fractionation; D002462:Cell Membrane / Q000032:analysis*; D002501:Centrifugation, Zonal; D002784:Cholesterol / Q000032:analysis; D002849:Chromatography, Gas; D003594:Cytoplasmic Granules / Q000032:analysis*; D004589:Electrophoresis, Disc; D007962:Leukocytes / Q000032:analysis* / Q000166:cytology; D008854:Microscopy, Electron; D008970:Molecular Weight; D010743:Phospholipids / Q000032:analysis; D011506:Proteins / Q000032:analysis; D011817:Rabbits
D010743:Phospholipids; D011506:Proteins; D002784:Cholesterol