title: Inducible uptake system for  -carboxy-cis, cis-muconate in a permeability mutant of Pseudomonas putida.
abstract: A procedure for the large-scale enzymatic synthesis of beta-carboxymuconate is described. When used as a growth substrate, beta-carboxymuconate selected for mutant strains of Pseudomonas putida that were permeable to polycarboxylic acid intermediates of the beta-ketoadipate pathway. One mutant organism, strain PRS2110, was investigated in detail. It differed from the parental strain in that it possessed a beta-carboxymuconate uptake system that was formed when the compound was supplied exogenously to the cells. The uptake system was not induced by beta-carboxymuconate supplied endogenously during growth with p-hydroxybenzoate. These observations suggested that beta-carboxymuconate was contained within a physical compartment of enzymes during growth with p-hydroxybenzoate. Support for this hypothesis came from the demonstration that enzymes of the beta-ketoadipate pathway were held together by weak chemical interactions during the chromatography of crude extracts of benzoategrown P. putida on diethylaminoethyl-cellulose columns.
mesh_terms: D000272:Adipates / Q000378:metabolism*; D001565:Benzoates / Q000378:metabolism; D002262:Carboxy-Lyases / Q000378:metabolism; D002396:Catechols; D002463:Cell Membrane Permeability; D002474:Cell-Free System; D002701:Chloramphenicol / Q000494:pharmacology; D003470:Culture Media; D004790:Enzyme Induction; D005827:Genetics, Microbial; D006867:Hydrolases / Q000378:metabolism; D007535:Isomerases / Q000378:metabolism; D007651:Keto Acids / Q000378:metabolism; D007783:Lactones; D009154:Mutation; D010101:Oxygen Consumption; D010105:Oxygenases / Q000302:isolation & purification / Q000378:metabolism; D011549:Pseudomonas / Q000166:cytology / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D013386:Succinates / Q000378:metabolism
chemical_list: D000272:Adipates; D001565:Benzoates; D002396:Catechols; D003470:Culture Media; D007651:Keto Acids; D007783:Lactones; D013386:Succinates; D002701:Chloramphenicol; D010105:Oxygenases; D006867:Hydrolases; D002262:Carboxy-Lyases; D007535:Isomerases