In vivo formation of H2O2 in red cells during exposure to hyperoxia.
Chow-fed and tocopherol-deficient mice were given aminotriazole (AT), exposed to 100% O(2) at 60 pounds per square inch absolute for 1 hr (OHP), and red blood cells were assayed for catalase activity and lipid peroxide levels. A decrease of catalase activity (CA) in the presence of AT can be taken as evidence of excess formation or accumulation of H(2)O(2). No differences of CA were observed among chow-fed mice, with or without AT and/or OHP. Tocopherol-deficient mice with AT had lower CA (0.174+/-0.040) than chow-fed mice with AT (0.225+/-0.028) P < 0.01. Tocopherol-deficient mice with AT exposed to OHP had even lower CA, 0.137+/-0.024, P < 0.01.The data are consistent with the hypothesis that H(2)O(2) is formed or accumulated in excess in red cells of tocopherol-deficient mice, an effect that is enhanced in the presence of hyperoxia. They imply that tocopherol plays a role in the detoxification of H(2)O(2).
D000818:Animals; D002374:Catalase / Q000378:metabolism; D004912:Erythrocytes / Q000201:enzymology / Q000378:metabolism*; D005260:Female; D006861:Hydrogen Peroxide / Q000096:biosynthesis*; D006931:Hyperbaric Oxygenation; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010100:Oxygen / Q000506:poisoning*; D014230:Triazoles / Q000494:pharmacology; D014811:Vitamin E Deficiency / Q000097:blood / Q000201:enzymology / Q000378:metabolism*
D014230:Triazoles; D006861:Hydrogen Peroxide; D002374:Catalase; D010100:Oxygen