Mellein and 4-hydroxymellein production by Aspergillus ochraceus Wilhelm.
Mellein and 4-hydroxymellein are isocoumarin compounds produced by Aspergillus ochraceus Wilhelm. They are structurally similar to the dihydroisocoumarin moiety of ochratoxin A, a toxic metabolite of the same fungus, and they possibly have similar biological properties. Production of mellein and 4-hydroxymellein on synthetic media and natural solid substrates was determined. Several carbon and nitrogen sources supported production of these metabolites in stationary culture. Additional zinc and molybdenum increased production of both metabolites in stationary culture, but were not required for maximum production in shaken culture. Mellein and 4-hydroxymellein were produced on yellow corn, but neither was produced on wheat, peanuts, or soybeans.
D000704:Analysis of Variance; D010367:Arachis; D001230:Aspergillus / Q000254:growth & development / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D002855:Chromatography, Thin Layer; D003374:Coumarins / Q000032:analysis / Q000096:biosynthesis*; D003470:Culture Media; D005374:Filtration; D005971:Glutamates / Q000378:metabolism; D008982:Molybdenum / Q000494:pharmacology; D009584:Nitrogen / Q000378:metabolism; D009793:Ochratoxins / Q000096:biosynthesis; D013025:Glycine max; D013053:Spectrophotometry; D013395:Sucrose / Q000378:metabolism; D013997:Time Factors; D014131:Trace Elements; D014908:Triticum; D014466:Ultraviolet Rays; D003313:Zea mays; D015032:Zinc / Q000494:pharmacology
D003374:Coumarins; D003470:Culture Media; D005971:Glutamates; D009793:Ochratoxins; D014131:Trace Elements; D013395:Sucrose; D008982:Molybdenum; D015032:Zinc; D009584:Nitrogen