Chromatography of Venezuelan equine encephalomyelitis virus strains on calcium phosphate.
Column chromatography of selected Venezuelan equine encephalomyelitis (VEE) viruses on calcium phosphate gel offered a simple and reproducible method for examination of biochemical characteristics and relatedness of strains within the VEE complex. Members of antigenic subgroup I demonstrated a series of elution profiles within a narrow range of 0.22 to 0.25 M phosphate buffer. Members of antigenic subgroups II, III, and IV differed substantially among themselves and viruses of antigenic subgroup I. These differences in elution behavior may contribute to understanding of observed differences in biological behavior and antigenic variation among VEE viruses.
D002130:Calcium Phosphates; D002845:Chromatography; D002852:Chromatography, Ion Exchange; D004666:Encephalitis Virus, Venezuelan Equine / Q000145:classification / Q000276:immunology / Q000302:isolation & purification; D004668:Encephalitis Viruses / Q000145:classification*; D005782:Gels; D006386:Hemagglutination Tests; D006389:Hemagglutinins, Viral / Q000032:analysis; D008722:Methods; D012703:Serotyping; D014776:Virus Cultivation
D002130:Calcium Phosphates; D005782:Gels; D006389:Hemagglutinins, Viral