Halophilic amylase from a moderately halophilic Micrococcus.
A moderately halophilic Micrococcus sp., isolated from unrefined solar salt, produced a considerable amount of extracellular dextrinogenic amylase when cultivated aerobically in media containing 1 to 3 m NaCl. The Micrococcus amylase had maximal activity at pH 6 to 7 in 1.4 to 2 m NaCl or KCl at 50 C. Calcium ion and a high concentration of NaCl or KCl were essential for activity and stability of the amylase. The salt response of the amylase depended greatly on the pH and temperature of the enzyme assay.
D000332:Aerobiosis; D000681:Amylases / Q000096:biosynthesis* / Q000378:metabolism; D002118:Calcium / Q000494:pharmacology; D003470:Culture Media; D003956:Dialysis; D005947:Glucose / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008837:Micrococcus / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D011189:Potassium Chloride / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013053:Spectrophotometry; D013696:Temperature
D003470:Culture Media; D012965:Sodium Chloride; D011189:Potassium Chloride; D000681:Amylases; D005947:Glucose; D002118:Calcium