title: Selective inhibition of the synthesis of Sindbis virion proteins by an inhibitor of chymotrypsin.
abstract: Treatment of chick embryo fibroblasts infected with Sindbis virus with TPCK, the choloromethyl ketone derivative of tosyl-phenylalanine and an inhibitor of chymotrypsin, resulted in reduced synthesis of viral structural proteins and the accumulation of a high-molecular-weight polypeptide, thought to be a precursor. The analogous inhibitor of trypsin, TLCK, the chloromethyl ketone derivative of tosyllysine, had no such effect.
mesh_terms: D000818:Animals; D001103:Arboviruses / Q000378:metabolism*; D002478:Cells, Cultured; D002642:Chick Embryo; D002918:Chymotrypsin / Q000037:antagonists & inhibitors*; D004589:Electrophoresis, Disc; D005347:Fibroblasts; D007930:Leucine / Q000378:metabolism; D008970:Molecular Weight; D012845:Sindbis Virus / Q000254:growth & development / Q000378:metabolism; D013450:Sulfones / Q000494:pharmacology; D014050:Toluene / Q000494:pharmacology; D014105:Tosyl Compounds / Q000494:pharmacology; D014316:Tritium; D014361:Trypsin Inhibitors / Q000494:pharmacology; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis*; D014776:Virus Cultivation
chemical_list: D013450:Sulfones; D014105:Tosyl Compounds; D014361:Trypsin Inhibitors; D014764:Viral Proteins; D014316:Tritium; D014050:Toluene; D002918:Chymotrypsin; D007930:Leucine