Composition and size of Shope fibroma virus deoxyribonucleic acid.
Deoxyribonucleic acid (DNA) extracted from purified virions of Shope fibroma virus (SFV) (by using DNA from Microccocus lysodeikticus as marker) had a buoyant density of 1.6996 +/- 0.0003 g/ml), hence a guanine plus cytosine (G + C) content of 40.4 +/- 0.3%, which is close to the G + C content of the DNA of susceptible rabbit cells (40.9 +/- 0.4%) and different from that of vaccinia virus DNA (35.5 +/- 0.4%). For the determination of the molecular weight of DNA, SFV and vaccinia purified virions, treated with Pronase and detergent, were cosedimented in sucrose density gradients. Results showed that SFV-DNA has a molecular weight of about 153 x 10(6) daltons. By electron microscopy, only one molecule corresponding to this value was observed (its length was 80.3 mum). The others had a median size of 49.8 mum +/- 0.9.
D000818:Animals; D002247:Carbon Isotopes; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002501:Centrifugation, Zonal; D003596:Cytosine / Q000032:analysis; D004247:DNA / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D003902:Detergents; D006147:Guanine / Q000032:analysis; D007668:Kidney; D008837:Micrococcus / Q000032:analysis; D008854:Microscopy, Electron; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D009695:Nucleic Acid Renaturation; D011212:Poxviridae / Q000032:analysis*; D011402:Pronase; D011817:Rabbits; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014412:Tumor Virus Infections / Q000382:microbiology*; D014616:Vaccinia virus / Q000032:analysis
D002247:Carbon Isotopes; D004279:DNA, Viral; D003902:Detergents; D014316:Tritium; D006147:Guanine; D003596:Cytosine; D004247:DNA; D011402:Pronase; D013936:Thymidine