title: Ionic permeability changes as the basis of the thermal dependence of the resting potential in barnacle muscle fibres.
abstract: 1. The thermal dependence of the resting potential of isolated barnacle muscle fibres was larger (1-2 mV/ degrees C) than predicted by Nernst's equation (about 0.2 mV/ degrees C). A comparative study was made of the influence on thermal dependence of parameters related to (a) passive permeability and to (b) Na extrusion.2. High [K](o) decreased the thermal dependence reversibly. [K(i)], [Na](i) and [Cl](i) were determined by chemical analysis, and Goldman's equation was fitted to data relating V to [K](o) at different temperatures, in the presence and absence of ouabain 5 x 10(-5)M. In both cases the behaviour of V when T was lowered from 20 to 4 degrees C was accounted for by increases in the calculated P(Na/PK) and P(Cl/PK) (from 0.006 to 0.043 and from 0.17 to 0.34 on the average, respectively.)3. Other parameters related to passive permeability (and which caused reversible depolarization): decreased [Cl](o) (methanesulphonate or gluconate substituted), and decreased pH(o) (below 5.0), also decreased the thermal dependence reversibly.4. Inhibitors (ouabain 5 x 10(-5)M, cyanide 2-10 x 10(-3)M, 2,4-dinitrophenol 2 x 10(-4)M) externally applied did not affect either resting potential or its thermal dependence for several hours.5. Increasing [Na](i) three- to fourfold by intracellular injection decreased both resting potential and its thermal dependence.6. Although a small effect by a Na electrogenic pump cannot be excluded, the largest part of the thermal effect on the resting potential is concluded to depend on temperature-induced variations in relative ionic permeabilities to cations and anions. A model is proposed which can account for the data assuming that (a) each permeant ion associates to a separate site in the membrane, and (b) the ion-site equilibrium is temperature-dependent.
mesh_terms: D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects; D002712:Chlorides / Q000378:metabolism; D003486:Cyanides / Q000494:pharmacology; D004594:Electrophysiology; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D009132:Muscles / Q000502:physiology*; D009210:Myofibrils / Q000378:metabolism; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D013696:Temperature; D001468:Thoracica / Q000502:physiology
chemical_list: D002712:Chlorides; D003486:Cyanides; D010042:Ouabain; D012964:Sodium; D011188:Potassium