Mast cells from human respiratory tissue and their in vitro reactivity.
Human respiratory mast cells, which were obtained coincidentally with diagnostic bronchial brush biopsy, were maintained under conditions for short-term tissue culture. Most mast cells were viable and degranulated on exposure to antibody to immunoglobulin E or to the mast cell degranulating agent compound 48-80. The degranulation of human mast cells is characteristically an intracellular process with no extracellular extrusion of granules.
D000818:Animals; D001706:Biopsy; D001769:Blood; D001980:Bronchi / Q000166:cytology*; D002478:Cells, Cultured; D003470:Culture Media; D003594:Cytoplasmic Granules; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007073:Immunoglobulin E / Q000037:antagonists & inhibitors; D066298:In Vitro Techniques; D008171:Lung Diseases / Q000473:pathology; D008175:Lung Neoplasms / Q000473:pathology; D008407:Mast Cells / Q000166:cytology*; D011817:Rabbits; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D003470:Culture Media; D007106:Immune Sera; D007073:Immunoglobulin E; D003189:p-Methoxy-N-methylphenethylamine