Morphine-3-succinyl--bovine serum albumin: an immunogenic hapten-protein conjugate.
Morphine-3-hemisuccinate was synthesized by reaction of morphine with succinic anhydride. This compound was conjugated to bovine serum albumin by the mixed anhydride method, and the degree of conjugation was determined by base hydrolysis of the conjugate, extraction, and measurement of free morphine. An average of 6.5 molecules of morphine were conjugated to each molecule of protein. Eleven rabbits immunized with varying doses of the conjugate were producing antibody 8 weeks later, as determined by a modification of the ammonium sulfate method, which measures primary binding of antigen by antibody.
D000818:Animals; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D002247:Carbon Isotopes; D002852:Chromatography, Ion Exchange; D006241:Haptens; D006868:Hydrolysis; D007114:Immunization; D009020:Morphine / Q000032:analysis; D011485:Protein Binding; D011817:Rabbits / Q000276:immunology; D012710:Serum Albumin, Bovine; D013057:Spectrum Analysis; D013386:Succinates
D002247:Carbon Isotopes; D006241:Haptens; D013386:Succinates; D012710:Serum Albumin, Bovine; D009020:Morphine