title: p-Toluenethiol as an initiator of autolysis in bakers' yeast.
abstract: Cysteine or dithiothreitol enhances the rate of autolysis in toluene-treated yeast. p-Toluenethiol alone is even more effective and is recommended for the isolation of beta-fructofuranosidase. This suggests a more general application of p-toluenethiol in the isolation of enzymes from yeast.
mesh_terms: D001329:Autolysis; D002473:Cell Wall / Q000187:drug effects / Q000201:enzymology; D003408:Cresols / Q000494:pharmacology*; D003545:Cysteine / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008722:Methods; D012440:Saccharomyces / Q000187:drug effects* / Q000201:enzymology; D013393:Sucrase / Q000302:isolation & purification / Q000378:metabolism; D013997:Time Factors; D014050:Toluene / Q000494:pharmacology
chemical_list: D003408:Cresols; D014050:Toluene; D013393:Sucrase; D003545:Cysteine; D004229:Dithiothreitol