Evidence for a phosphoenolpyruvate-dependent sugar phosphotransferase in Mycoplasma strain Y.
The uptake of (14)C-alpha-methyl-d-glucoside (alphaMG) by washed cells of Mycoplasma strain Y was found to be dependent on the supply of metabolic energy. Glycerol or d-mannose, but not l-lactate, would serve as an energy source. Uptake was inhibited by fluoride, iodoacetate, and arsenate, but not by 2,4-dinitrophenol. d-Glucose was inhibitory, presumably by competing for the transport system. The initial product of accumulation had the properties of a phosphate ester of alphaMG. The proportion of free alphaMG in the cells increased with time, until a steady state was reached in which uptake was balanced by the efflux of free alphaMG from the cells. Broken-cell preparations catalyzed a phosphoenolpyruvate-dependent phosphorylation of alphaMG and of d-glucose.
D000255:Adenosine Triphosphate; D001151:Arsenic / Q000494:pharmacology; D001693:Biological Transport, Active; D002247:Carbon Isotopes; D002374:Catalase / Q000378:metabolism; D002384:Catalysis; D004140:Dinitrophenols / Q000494:pharmacology; D005459:Fluorides / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006027:Glycosides / Q000378:metabolism; D007461:Iodoacetates / Q000494:pharmacology; D007773:Lactates / Q000378:metabolism; D008358:Mannose / Q000378:metabolism; D008851:Micropore Filters; D009174:Mycoplasma / Q000201:enzymology* / Q000378:metabolism; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D010728:Phosphoenolpyruvate; D010770:Phosphotransferases / Q000378:metabolism*
D002247:Carbon Isotopes; D004140:Dinitrophenols; D006027:Glycosides; D007461:Iodoacetates; D007773:Lactates; D010728:Phosphoenolpyruvate; D000255:Adenosine Triphosphate; D002374:Catalase; D010770:Phosphotransferases; D005947:Glucose; D001151:Arsenic; D005990:Glycerol; D008358:Mannose; D005459:Fluorides