Antigen binding and capping by lymphocytes of genetic nonresponder mice.
Radioautographic study of the binding of GAT-(125)I to spleen cells of genetic responder and nonresponder mice demonstrates that among mice not injected with antigen all strains have approximately the same number of antigen-binding cells; after injection with antigen the number of antigen-binding cells increases in responders but not in nonresponders. Nonresponders are shown to make antibody after injection with GAT complexed with an immunogenic carrier, demonstrating the presence of potentially functional B cells in responders and nonresponders alike. When incubated in the warm, antigen-binding cells of both responders and nonresponders concentrate antigen at one pole of the cell, forming caps.
D000818:Animals; D000917:Antibody Formation; D000941:Antigens; D001345:Autoradiography; D001665:Binding Sites; D006433:Hemocyanins; D007125:Immunogenetics; D007456:Iodine Isotopes; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains / Q000276:immunology*; D012908:Snails; D013154:Spleen / Q000166:cytology / Q000276:immunology*
D000941:Antigens; D007456:Iodine Isotopes; D006433:Hemocyanins