Genetic control of thymus-derived cell function. I. In vitro DNA synthetic response of normal mouse spleen cells stimulated by the mitogens concanavalin A and phytohemagglutinin.
Concanavalin A- or phytohemagglutinin-stimulated DNA synthetic responses of 1 million normal mouse spleen cells in vitro were significantly different among various inbred strains. BALB/cJ (H-2(d)) responded better than C57BL/6J (H-2(b)) spleen cells, and the responses of C3H/HeJ or AKR/J (both H-2(k)) cells were intermediate. These responses, measured as the increment in thymidine-(3)H incorporation of mitogen-stimulated compared with unstimulated cultures, varied according to the number of cells cultured or the mitogen concentration. BALB/c spleens had the highest proportion of theta-positive cells, but no direct relationship between the proportion of theta-positive cells and the DNA synthetic response was observed. (BALB/cJ x C57BL/6)F(1) spleen cells responsed as well as BALB/c cells. Responses of spleen cells from (F(1) x C57BL/6) backcross littermates varied over a range equal to, or greater than, that of BALB/c and C57BL/6 cells. There was no correlation between H-2 specificity (H-2(bd) or H-2(bb)) or sex and the mitogen-stimulated DNA synthetic response of backcross animals. Con A- and PHA-stimulated responses of individual backcross animals were positively correlated with the level of thymidine-(8)H incorporation by unstimulated spleen cells. These results are consistent with autosomal dominant, non-H-2-linked, polygenic control of the mitogen-stimulated in vitro DNA synthetic response of mouse spleen cells.
D000818:Animals; D000921:Antibody-Producing Cells; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003433:Crosses, Genetic; D003601:Cytotoxicity Tests, Immunologic; D004247:DNA / Q000096:biosynthesis*; D005260:Female; D005799:Genes, Dominant; D007125:Immunogenetics; D066298:In Vitro Techniques; D037102:Lectins / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains / Q000276:immunology / Q000378:metabolism*; D013154:Spleen / Q000166:cytology / Q000378:metabolism*; D013268:Stimulation, Chemical; D013936:Thymidine / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology; D014316:Tritium
D037102:Lectins; D014316:Tritium; D003208:Concanavalin A; D004247:DNA; D013936:Thymidine