Reovirus-specific ribonucleic acid from polysomes of infected L cells.
Polysomes from reovirus-infected L cells were analyzed. Although all 10 transcription products of the viral genome were represented in polysomes and presumably can be translated, fewer than 10 reovirus-specific polypeptides were detected in infected cells. The 5'-terminal sequence of all species of reovirus ribonucleic acid (RNA) from polysomes was ppGpYp, as previously found for the genome double-stranded RNA and the in vitro single-stranded products of the virion-associated RNA polymerase. RNA isolated from both heavy (>30 ribosomes) and light (5 to 8 ribosomes) polysomes includes similar amounts of large, medium, and small classes of reovirus single-stranded RNA, suggesting that linkage of the genome segments observed in virions may occur at the single-strand stage of RNA replication.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002848:Chromatography, DEAE-Cellulose; D004589:Electrophoresis, Disc; D005815:Genetic Code; D008040:Genetic Linkage; D007739:L Cells / Q000032:analysis* / Q000378:metabolism; D051379:Mice; D009693:Nucleic Acid Hybridization; D010443:Peptide Chain Termination, Translational; D010455:Peptides / Q000032:analysis; D010759:Phosphorus Isotopes; D012316:RNA Nucleotidyltransferases / Q000378:metabolism; D012335:RNA, Ribosomal / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis* / Q000096:biosynthesis; D012087:Reoviridae / Q000032:analysis* / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D012270:Ribosomes / Q000032:analysis*; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
D010455:Peptides; D010759:Phosphorus Isotopes; D012335:RNA, Ribosomal; D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium; D012316:RNA Nucleotidyltransferases; D014529:Uridine