title: Biochemical and genetic characterization of  -glucosidase mutants in Saccharomyces lactis.
abstract: Mutants with reduced activity for beta-glucosidase (beta-d-glucoside glucohydrolase EC 3.2.1.21) were isolated from the haploid yeast Saccharomyces lactis. Tetrad analysis indicated that in each mutant a single genetic factor, closely linked or allelic to the structural gene for beta-glucosidase (B locus), is responsible for the decreased activity. beta-Glucosidases produced by wild-type and mutant strains are similar in molecular size and charge but differ in catalytic properties, thermal stability, and serological specificity, indicating that mutants are in the structural gene. All mutants retained their capacity to be induced by either methyl-beta-d-glucoside or glucose. In all cases, the mutant phenotype was dominant in heterozygous diploids.
mesh_terms: D000818:Animals; D000937:Antigen-Antibody Reactions; D002499:Centrifugation, Density Gradient; D002848:Chromatography, DEAE-Cellulose; D004355:Drug Stability; D004592:Electrophoresis, Starch Gel; D004790:Enzyme Induction; D005260:Female; D005796:Genes; D005799:Genes, Dominant; D005827:Genetics, Microbial; D005947:Glucose / Q000494:pharmacology; D005959:Glucosidases / Q000096:biosynthesis / Q000302:isolation & purification; D006358:Hot Temperature; D007700:Kinetics; D008540:Meiosis; D009154:Mutation; D010641:Phenotype; D011817:Rabbits / Q000276:immunology; D011830:Radiation Effects; D012440:Saccharomyces / Q000201:enzymology* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D005959:Glucosidases; D005947:Glucose