Effect of monovalent cations on the malic enzyme from the extreme halophile, Halobacterium cutirubrum.
The malic enzyme from Halobacterium cutirubrum requires monovalent cations for both activation and stabilization. NaCl, the best stabilizer, is ineffective as activator; NH(4)Cl, the best activator, is a poor stabilizer. These results support the idea that the roles of salts in both processes are different.
D000643:Ammonium Chloride / Q000494:pharmacology*; D002474:Cell-Free System; D002712:Chlorides; D003470:Culture Media; D004789:Enzyme Activation; D006217:Halobacterium / Q000201:enzymology* / Q000254:growth & development; D008094:Lithium / Q000494:pharmacology*; D008291:Malate Dehydrogenase / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology*; D013053:Spectrophotometry
D002712:Chlorides; D003470:Culture Media; D000643:Ammonium Chloride; D012965:Sodium Chloride; D011189:Potassium Chloride; D008094:Lithium; D008291:Malate Dehydrogenase