Cuticle deposition in imaginal disks: effects of juvenile hormone and fat body in vitro.
Wing disks from the last larval instar of the Indian meal moth, Plodia interpunctella (Hübner), were successfully cultured in modified Grace's medium. 20-Hydroxyecdysone induced cuticle deposition in these disks in vitro. This response was enhanced by treating the medium with larval fat body and was inhibited by application of juvenile hormone.
D000273:Adipose Tissue / Q000502:physiology*; D000818:Animals; D046508:Culture Techniques; D004440:Ecdysone / Q000494:pharmacology*; D007313:Insecta; D007447:Invertebrate Hormones / Q000494:pharmacology; D007605:Juvenile Hormones / Q000494:pharmacology*; D007814:Larva; D012867:Skin / Q000187:drug effects*; D014921:Wings, Animal
D007447:Invertebrate Hormones; D007605:Juvenile Hormones; D004440:Ecdysone