title: Loss of the penicillinase plasmid after treatment of Staphylococcus aureus with sodium dodecyl sulfate.
abstract: Two strains of Staphylococcus aureus (PC1 and 196E), when grown in medium containing 0.002% sodium dodecyl sulfate (SDS), showed from 96.1 to 100% loss of the ability to produce penicillinase. Resistance to cadmium and zinc was lost concomitantly with the ability to produce penicillinase. A comparison of the rate of curing by SDS with the curing effects exerted by elevated temperature and ethidium bromide suggested that SDS is a more effective plasmid curing agent for susceptible strains of S. aureus than methods in use at present.
mesh_terms: D002104:Cadmium / Q000494:pharmacology; D003470:Culture Media; D004352:Drug Resistance, Microbial; D005827:Genetics, Microbial; D006358:Hot Temperature; D010405:Penicillinase / Q000096:biosynthesis*; D010617:Phenanthridines / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology*; D013210:Staphylococcus / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D013431:Sulfates / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology
chemical_list: D003470:Culture Media; D010617:Phenanthridines; D013431:Sulfates; D002104:Cadmium; D012964:Sodium; D010405:Penicillinase; D015032:Zinc