Identification and quantitation of free ceramides in human platelets.
Free ceramides were isolated from human platelets. Their structures were unequivocally determined by gas-liquid chromatography-mass spectrometry of the trimethylsilyl ether derivatives. The major components were N-(palmitoyl) sphingosine, N-(stearoyl) sphingosine, N-(eicosanoyl) sphingosine, N-(docosanoyl) sphingosine, N-(tetracosanoyl) sphingosine, and N-(tetracosenoyl) sphingosine. Sphinganine-and sphingadienine-containing ceramides as well as ceramides containing other unsaturated acids were also present. The amount of ceramides was determined by quantitative gas-liquid chromatography, using radioactive ceramide as internal standard and synthetic crystalline ceramides for comparison of peak areas. The concentration of ceramides was found to be 1.31 micro g/10(9) platelets or 0.47 micro g/mg of platelet protein.
D001792:Blood Platelets / Q000032:analysis*; D001798:Blood Proteins / Q000032:analysis; D002247:Carbon Isotopes; D002518:Ceramides / Q000032:analysis / Q000097:blood / Q000302:isolation & purification*; D002845:Chromatography; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000032:analysis; D006801:Humans; D008055:Lipids / Q000032:analysis; D013058:Mass Spectrometry; D010169:Palmitic Acids / Q000032:analysis; D013110:Sphingosine / Q000032:analysis; D013229:Stearic Acids / Q000032:analysis
D001798:Blood Proteins; D002247:Carbon Isotopes; D002518:Ceramides; D005227:Fatty Acids; D008055:Lipids; D010169:Palmitic Acids; D013229:Stearic Acids; D013110:Sphingosine