Selective inhibition of the synthesis of Sindbis virion proteins by an inhibitor of chymotrypsin.
Treatment of chick embryo fibroblasts infected with Sindbis virus with TPCK, the choloromethyl ketone derivative of tosyl-phenylalanine and an inhibitor of chymotrypsin, resulted in reduced synthesis of viral structural proteins and the accumulation of a high-molecular-weight polypeptide, thought to be a precursor. The analogous inhibitor of trypsin, TLCK, the chloromethyl ketone derivative of tosyllysine, had no such effect.
D000818:Animals; D001103:Arboviruses / Q000378:metabolism*; D002478:Cells, Cultured; D002642:Chick Embryo; D002918:Chymotrypsin / Q000037:antagonists & inhibitors*; D004589:Electrophoresis, Disc; D005347:Fibroblasts; D007930:Leucine / Q000378:metabolism; D008970:Molecular Weight; D012845:Sindbis Virus / Q000254:growth & development / Q000378:metabolism; D013450:Sulfones / Q000494:pharmacology; D014050:Toluene / Q000494:pharmacology; D014105:Tosyl Compounds / Q000494:pharmacology; D014316:Tritium; D014361:Trypsin Inhibitors / Q000494:pharmacology; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis*; D014776:Virus Cultivation
D013450:Sulfones; D014105:Tosyl Compounds; D014361:Trypsin Inhibitors; D014764:Viral Proteins; D014316:Tritium; D014050:Toluene; D002918:Chymotrypsin; D007930:Leucine