title: Occurrence of taurine:  -ketoglutarate aminotransferase in bacterial extracts.
abstract: High activity of taurine:alpha-ketoglutarate aminotransferase was found exclusively in cell-free extracts of Achromobacter superficialis and A. polymorph. The former was chosen for characterization of the enzymatic reaction. The enzyme activity was enhanced by addition of beta-alanine to the growth medium. The product from alpha-ketoglutarate was identified as l-glutamate. Another product has been isolated, purified, and identified as sulfoacetaldehyde (2-oxoethanesulfonate), a deamination product from taurine, by comparison between the 2,4-dinitrophenylhydrazones of the synthetic and enzymatic products on the basis of studies by paper chromatography, by visible, infrared, and nuclear magnetic resonance spectrophotometries, and by elemental analysis. This enzymatic transamination was found to proceed stoichiometrically and reversibly as follows: NH(2).CH(2).CH(2).SO(3)H + HOOC.CH(2).CH(2).CO.COOH right harpoon over left harpoon OHC.CH(2).SO(3)H + HOOC.CH(2).CH(2).CH(NH(2)).COOH.
mesh_terms: D000409:Alanine / Q000494:pharmacology; D000410:Alanine Transaminase / Q000378:metabolism; D000421:Alcaligenes / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis; D000645:Ammonium Sulfate; D001322:Autoanalysis; D055598:Chemical Phenomena; D002621:Chemistry; D002854:Chromatography, Paper; D003641:Deamination; D004140:Dinitrophenols / Q000096:biosynthesis; D005971:Glutamates / Q000096:biosynthesis; D006835:Hydrazones / Q000096:biosynthesis; D007259:Infrared Rays; D007656:Ketoglutaric Acids / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013045:Species Specificity; D013053:Spectrophotometry; D013451:Sulfonic Acids / Q000096:biosynthesis; D013654:Taurine / Q000378:metabolism* / Q000494:pharmacology; D000637:Transaminases / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D004140:Dinitrophenols; D005971:Glutamates; D006835:Hydrazones; D007656:Ketoglutaric Acids; D013451:Sulfonic Acids; D013654:Taurine; D000637:Transaminases; D000410:Alanine Transaminase; D000409:Alanine; D000645:Ammonium Sulfate