Electron microscope study of surface immunoglobulin-bearing human tonsil cells.
Surface immunoglobulin-bearing cells were selected from suspensions of human tonsil cells by the reverse immune cytoadherence technique. The method employed a hybrid antibody directed against Ig on lymphoid cells and against ferritin bound to sheep red blood cells (SRBC). Only 6% of the cells formed rosettes. When subjected to electron microscopy they were shown to consist of a morphologically heterogeneous population of cells. However, most cells in the center of rosettes showed ribosome-associated endoplasmic reticulum (RER) and polyribosomes. Usually these organelles were located in close proximity to membrane sites where a 400-600 A bridge was resolved between the lymphocyte and the ferritin particle on the SRBC. The bridge is postulated to consist at least in part of Ig. Only 50% of the plasma cells formed rosettes and bridges could not be resolved. The surface of the plasma cells within rosettes differed from that of plasma cells which had not reacted with ferritin-coated sheep erythrocytes. The incidence of plasma cells and gamma-globulin-bearing lymphoid cells was corroborated with the help of fluorescent antibody techniques.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D002462:Cell Membrane / Q000276:immunology*; D004721:Endoplasmic Reticulum; D004912:Erythrocytes / Q000276:immunology; D005293:Ferritins; D006801:Humans; D006824:Hybridization, Genetic; D007104:Immune Adherence Reaction; D007111:Immunity, Cellular; D007074:Immunoglobulin G; D007136:Immunoglobulins; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000166:cytology* / Q000276:immunology; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D014066:Palatine Tonsil / Q000166:cytology* / Q000276:immunology; D010950:Plasma Cells / Q000166:cytology; D012756:Sheep
D000906:Antibodies; D007074:Immunoglobulin G; D007136:Immunoglobulins; D005293:Ferritins