title: Cell surface immunoglobulin. V. Release from murine splenic lymphocytes.
abstract: Turnover and release of cell surface Ig and secretion of total intracellular Ig has been studied in small lymphocytes from normal mouse spleen. The major findings to emerge are: (a) small lymphocytes secrete 8S IgM and IgG. A small portion of the 8S IgM, but virtually none of the IgG appears to have a cell surface phase. (b) Cell surface IgM is actively turned over with a half-life of 6-8 hr, and turnover can be accounted for by release into the incubation medium. Release is temperature dependent. (c) Released cell surface Ig is noncovalently bound to a fragment of plasma membrane. (d) H-2 antigens are not released during short-term incubation. Based on the above findings, we propose a model for the transport and release of both cell surface and conventionally secreted Ig.
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000276:immunology*; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D002725:Chloroform / Q000494:pharmacology; D002845:Chromatography; D003513:Cycloheximide / Q000494:pharmacology; D007074:Immunoglobulin G / Q000378:metabolism; D007136:Immunoglobulins / Q000302:isolation & purification*; D007456:Iodine Isotopes; D007773:Lactates; D050356:Lipid Metabolism; D008074:Lipoproteins / Q000032:analysis; D008214:Lymphocytes / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D010544:Peroxidases / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D013156:Splenectomy; D014316:Tritium; D014443:Tyrosine / Q000378:metabolism
chemical_list: D007074:Immunoglobulin G; D007136:Immunoglobulins; D007456:Iodine Isotopes; D007773:Lactates; D008074:Lipoproteins; D014316:Tritium; D014443:Tyrosine; D002725:Chloroform; D003513:Cycloheximide; D010544:Peroxidases