RNA metabolism in HeLa cells at reduced temperature. II. Steps in the processing of transfer RNA.
Incubation of HeLa cells at 24 degrees C results in the modification of the processing of pre-tRNA to tRNA. Both methylation and size reduction were shown to take place in vitro when purified pre-tRNA was subjected to processing in a cytoplasmic extract of HeLa cells The migration of pre-tRNA from the nucleus to the cytoplasm was not significantly altered at 24 degrees C
D002458:Cell Fractionation; D002467:Cell Nucleus / Q000378:metabolism; D002474:Cell-Free System; D003593:Cytoplasm / Q000378:metabolism; D004589:Electrophoresis, Disc; D006367:HeLa Cells / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D008745:Methylation; D010759:Phosphorus Isotopes; D012343:RNA, Transfer / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D012436:S-Adenosylmethionine / Q000378:metabolism; D013696:Temperature; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D010759:Phosphorus Isotopes; D014316:Tritium; D012436:S-Adenosylmethionine; D012343:RNA, Transfer; D008715:Methionine; D014529:Uridine