The release of prostaglandins and rabbit aorta contracting substance (RCS) from rabbit spleen and its antagonism by anti-inflammatory drugs.
1. Slices of rabbit spleen generate rabbit aorta contracting substance (RCS) and a prostaglandin of the E series when they are vibrated or stirred.2. The release of both substances is increased by arachidonic acid and inhibited by aspirin-like drugs.3. When the spleen effluent containing RCS and prostaglandin E is incubated for a further 3 min, the prostaglandin concentration increases and the RCS concentration declines.4. RCS may therefore be an unstable intermediate in the biosynthesis of prostaglandins.
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001011:Aorta / Q000187:drug effects; D001095:Arachidonic Acids / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology; D001681:Biological Assay; D002855:Chromatography, Thin Layer; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D010113:Oxyphenbutazone / Q000494:pharmacology; D011453:Prostaglandins / Q000378:metabolism*; D011817:Rabbits; D012636:Secretory Rate / Q000187:drug effects; D013154:Spleen / Q000187:drug effects*; D014020:Tissue Extracts / Q000378:metabolism*; D014050:Toluene / Q000494:pharmacology; D062367:ortho-Aminobenzoates / Q000494:pharmacology
D000893:Anti-Inflammatory Agents; D001095:Arachidonic Acids; D011453:Prostaglandins; D014020:Tissue Extracts; D062367:ortho-Aminobenzoates; D014050:Toluene; D010113:Oxyphenbutazone; D001241:Aspirin