Rapid complement fixation technique for estimating complement-fixing antigen elution profiles of viruses from gel filtration columns.
The complement fixation elution profiles of dengue-2 virus-infected suckling mouse brain supernatant fluids from Sephadex G-200 columns were compared by the antigen end-point titration procedure and by a complement dilution technique. The latter technique was found to be a quick method for approximating the complement-fixing antigen in eluates.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis / Q000302:isolation & purification; D001202:Ascitic Fluid / Q000276:immunology; D001921:Brain / Q000382:microbiology; D002850:Chromatography, Gel; D003168:Complement Fixation Tests; D003165:Complement System Proteins; D003716:Dengue Virus / Q000276:immunology*; D005069:Evaluation Studies as Topic; D006168:Guinea Pigs / Q000276:immunology; D051379:Mice / Q000276:immunology
D000941:Antigens; D000956:Antigens, Viral; D003165:Complement System Proteins