title: Effect of monovalent cations on the malic enzyme from the extreme halophile, Halobacterium cutirubrum.
abstract: The malic enzyme from Halobacterium cutirubrum requires monovalent cations for both activation and stabilization. NaCl, the best stabilizer, is ineffective as activator; NH(4)Cl, the best activator, is a poor stabilizer. These results support the idea that the roles of salts in both processes are different.
mesh_terms: D000643:Ammonium Chloride / Q000494:pharmacology*; D002474:Cell-Free System; D002712:Chlorides; D003470:Culture Media; D004789:Enzyme Activation; D006217:Halobacterium / Q000201:enzymology* / Q000254:growth & development; D008094:Lithium / Q000494:pharmacology*; D008291:Malate Dehydrogenase / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology*; D013053:Spectrophotometry
chemical_list: D002712:Chlorides; D003470:Culture Media; D000643:Ammonium Chloride; D012965:Sodium Chloride; D011189:Potassium Chloride; D008094:Lithium; D008291:Malate Dehydrogenase