title: Cellulases of Thermomonospora fusca and Streptomyces thermodiastaticus.
abstract: The cellulases of Streptomyces thermodiastaticus (strain 2Sts) and thermomonospora fusca (strain 190Th) were produced with carboxymethyl-cellulose (CMC) serving as the carbon source during growth. Both cellulases act by random internal hydrolysis of the CMC chain, producing cellobiose, glucose, and intermediate length oligosaccharides. Cellobiase was not detected in culture filtrates produced under these conditions.
mesh_terms: D000192:Actinomycetales / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism; D002854:Chromatography, Paper; D004187:Disaccharides / Q000096:biosynthesis; D005947:Glucose / Q000096:biosynthesis; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D003368:Gossypium; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008747:Methylcellulose / Q000378:metabolism; D009844:Oligosaccharides / Q000096:biosynthesis; D010209:Paper; D013302:Streptomyces / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D013696:Temperature
chemical_list: D004187:Disaccharides; D009844:Oligosaccharides; D002482:Cellulose; D008747:Methylcellulose; D006026:Glycoside Hydrolases; D005947:Glucose