Cell surface immunoglobulin. IV. Distribution among thymocytes, bone mrrow cells, and their derived populations.
Thymocytes, bone marrow cells, and their derived T and B cell populations were examined for the presence of Ig by the cell surface radioiodination technique. Both IgM and IgG were identified on bone marrow cells. Thymocytes and T cells had no detectable cell surface Ig. Radiolabeling of mixtures of B cells and thymocytes suggest that the method may detect as little as 250 molecules of Ig per cell. Based on these findings, we suggest that the T cell receptor for antigen is not a conventional tetrameric Ig.
D000178:Acrylamides; D000818:Animals; D000921:Antibody-Producing Cells; D001853:Bone Marrow / Q000276:immunology*; D001854:Bone Marrow Cells; D002462:Cell Membrane / Q000276:immunology*; D004586:Electrophoresis; D007111:Immunity, Cellular; D007074:Immunoglobulin G / Q000032:analysis; D007136:Immunoglobulins / Q000032:analysis*; D066298:In Vitro Techniques; D007456:Iodine Isotopes; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D012967:Sodium Dodecyl Sulfate; D013154:Spleen / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology*; D014316:Tritium; D014443:Tyrosine / Q000378:metabolism
D000178:Acrylamides; D007074:Immunoglobulin G; D007136:Immunoglobulins; D007456:Iodine Isotopes; D014316:Tritium; D012967:Sodium Dodecyl Sulfate; D014443:Tyrosine