The specific assay of arylsulphatase C, a rat liver microsomal marker enzyme.
Conditions based on previous assays with potassium p-acetylphenyl sulphate have been established for the specific assay of arylsulphatase C in rat tissues. The enzyme has optimum activity with 40mm substrate at pH8.0 in the presence of 0.1m-phosphate buffer. Under these conditions arylsulphatase C can be assayed without interference from the other arylsulphatase enzymes present and is useful as a marker for the endoplasmic reticulum in cell-fractionation studies.
D000818:Animals; D002021:Buffers; D002458:Cell Fractionation; D004721:Endoplasmic Reticulum / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D010710:Phosphates; D051381:Rats; D013429:Sulfatases / Q000032:analysis*; D013431:Sulfates
D002021:Buffers; D010710:Phosphates; D013431:Sulfates; D013429:Sulfatases