The amino acid sequence of the B-protein of bacteriophage ZJ-2.
1. Bacteriophage ZJ-2 is a filamentous male-specific coliphage. Methods are given for its preparation and separation into nucleic acid and the major coat-protein component (B-protein). 2. The determination of the amino acid sequence of the B-protein of bacteriophage ZJ-2 is described. The principal features of this aspect of the work were the special procedures dictated by the insolubility of the protein and the extreme insolubility of its fragments. The sequence is compared with that obtained by other workers for the coat protein of the related bacteriophage fd.
D000215:Acylation; D000595:Amino Acid Sequence; D002918:Chymotrypsin; D003090:Coliphages / Q000032:analysis*; D003488:Cyanogen Bromide; D004586:Electrophoresis; D009696:Nucleic Acids / Q000032:analysis; D010455:Peptides / Q000302:isolation & purification; D012967:Sodium Dodecyl Sulfate; D013386:Succinates; D013820:Thermolysin; D014357:Trypsin; D014764:Viral Proteins / Q000032:analysis*
D009696:Nucleic Acids; D010455:Peptides; D013386:Succinates; D014764:Viral Proteins; D012967:Sodium Dodecyl Sulfate; D002918:Chymotrypsin; D014357:Trypsin; D013820:Thermolysin; D003488:Cyanogen Bromide