Covalent coupling of pullulanase to an acrylic copolymer using a water soluble carbodi-imide.
D000178:Acrylamides; D000179:Acrylates; D001665:Binding Sites; D002234:Carbodiimides; D004754:Enterobacter / Q000201:enzymology*; D006026:Glycoside Hydrolases / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D008722:Methods; D011108:Polymers; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D011506:Proteins / Q000032:analysis
D000178:Acrylamides; D000179:Acrylates; D002234:Carbodiimides; D011108:Polymers; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; D011506:Proteins; D006026:Glycoside Hydrolases