Aryl hydrocarbon hydroxylase induction in human leukocytes.
A method for determining aryl hydrocarbon hydroxylase induction in human leukocytes is described. Leukocytes from healthy volunteers were cultured in the presence of phytohemagglutinin, a mitogen. Addition of 3-methylcholanthrene to 72-hour cultures induced a fourfold increase in aryl hydroxylase activity. In the absence of a mitogenic agent, 3-methylcholanthrene stimulation of increased enzymatic activity did not occur.
D002478:Cells, Cultured; D004790:Enzyme Induction; D006801:Humans; D006838:Hydrocarbons; D037102:Lectins; D007962:Leukocytes / Q000201:enzymology*; D008748:Methylcholanthrene / Q000494:pharmacology*; D006899:Mixed Function Oxygenases / Q000032:analysis*; D013997:Time Factors
D006838:Hydrocarbons; D037102:Lectins; D008748:Methylcholanthrene; D006899:Mixed Function Oxygenases