Stimulation of the production of unesterified fatty acids in nerve endings of guinea-pig brain in vitro by noradrenaline and 5-hydroxytryptamine.
1. Noradrenaline (1mm) and 5-hydroxytryptamine (1mm) stimulated the production of unesterified palmitate, oleate, stearate and arachidonate in nerve endings (synaptosomes) isolated from combined guinea-pig cerebral cortex and cerebellum. 2. Iproniazid phosphate (0.36mm) increased the concentrations of the same acids in osmotically ruptured synaptosomes. Further addition of 1mm-noradrenaline or 1mm-5-hydroxytryptamine reversed this increase. 3. Noradrenaline (0.01mm) stimulated the production of unesterified fatty acids in isolated synaptic membranes. 5-Hydroxytryptamine (0.01mm) stimulated the production of unesterified fatty acids in synaptic membranes and synaptic vesicles.
D000818:Animals; D001095:Arachidonic Acids / Q000096:biosynthesis; D001921:Brain / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000096:biosynthesis*; D006168:Guinea Pigs; D007490:Iproniazid / Q000494:pharmacology; D008297:Male; D009411:Nerve Endings / Q000187:drug effects*; D009638:Norepinephrine / Q000494:pharmacology*; D009829:Oleic Acids / Q000096:biosynthesis; D010169:Palmitic Acids / Q000096:biosynthesis; D012701:Serotonin / Q000494:pharmacology*; D013229:Stearic Acids / Q000096:biosynthesis; D013570:Synaptic Membranes / Q000378:metabolism; D013572:Synaptic Vesicles / Q000378:metabolism; D013574:Synaptosomes
D001095:Arachidonic Acids; D005230:Fatty Acids, Nonesterified; D009829:Oleic Acids; D010169:Palmitic Acids; D013229:Stearic Acids; D012701:Serotonin; D007490:Iproniazid; D009638:Norepinephrine