Transcription of the influenza ribonucleic acid genome by a virion polymerase. 3. Completeness of the transcription process.
The virion ribonucleic acid (RNA) polymerase of influenza strain A(0)/WS transcribed at least 81% of the viral genome in vitro. The polymerase is shown to be associated with each of the major size classes of the virion RNA-ribonucleoprotein complexes. Under optimal in vitro conditions, at least 45% of the RNA contained in a population of influenza virions was involved in a repetitive transcription process. The detectable proteins associated with enzymatically active complexes containing RNA, ribonucleoprotein, and polymerase have been identified by polyacrylamide gel electrophoresis.
D002499:Centrifugation, Density Gradient; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D009698:Nucleoproteins / Q000378:metabolism; D009975:Orthomyxoviridae / Q000201:enzymology* / Q000378:metabolism; D010759:Phosphorus Isotopes; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism; D012260:Ribonucleases; D014158:Transcription, Genetic; D014316:Tritium; D014500:Uracil Nucleotides / Q000378:metabolism; D014529:Uridine / Q000378:metabolism; D014764:Viral Proteins / Q000032:analysis / Q000378:metabolism
D009698:Nucleoproteins; D010759:Phosphorus Isotopes; D012367:RNA, Viral; D014500:Uracil Nucleotides; D014764:Viral Proteins; D014316:Tritium; D012321:DNA-Directed RNA Polymerases; D012260:Ribonucleases; D014529:Uridine