title: The effect of carbohydrates on the intestinal potentials of Cryptochiton stelleri.
abstract: 1. The effect of various monosaccharides on the potential difference across the intestine of the invertebrate, Cryptochiton stelleri, was studied using an everted sac technique.2. D-Glucose, when present in the mucosal solution, increased the transmural potential across the anterior intestine but had no effect on electrical potentials across the posterior intestine.3. D-Galactose, when present in the mucosal solution, increased the transmural potential across the posterior intestine but had no effect on electrical potentials across the anterior intestine.4. D-Fructose and D-mannose, metabolizable but non-transported sugars, increased anterior intestinal potentials but not posterior intestinal potentials.5. 3-O-methylglucose, an actively transported (across the anterior intestine) but non-metabolizable sugar, did not alter potentials across either the anterior or posterior intestine.6. The addition of D-glucose to the serosal solution increased anterior intestinal potentials, but the serosal addition of D-galactose did not affect posterior intestinal potentials.7. NaF, phlorizin and anoxia prevented the increase in potential across the anterior intestine and phlorizin (10(-4) but not 10(-6)M) and NaF but not anoxia prevented the increase in potential across the posterior intestine.8. It was suggested that the effect of glucose on anterior intestinal potentials was a metabolic effect, whereas the effect of galactose on posterior intestinal potentials was related to the active transport of that sugar from mucosa to serosa.
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D004068:Digestive System Physiological Phenomena; D005459:Fluorides / Q000494:pharmacology; D005632:Fructose / Q000494:pharmacology; D005690:Galactose / Q000378:metabolism / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism; D008358:Mannose / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects*; D008974:Mollusca / Q000502:physiology*; D009005:Monosaccharides / Q000494:pharmacology*; D010100:Oxygen; D010695:Phlorhizin / Q000494:pharmacology; D013997:Time Factors
chemical_list: D009005:Monosaccharides; D005632:Fructose; D010695:Phlorhizin; D005947:Glucose; D008358:Mannose; D005459:Fluorides; D010100:Oxygen; D005690:Galactose