Oxidative phosphorylation. The effect of anions on the inhibition by triethyltin of various mitochondrial functions, and the relationship between this inhibition and binding of triethyltin.
1. The binding of triethyltin to rat liver mitochondria is unaffected by the nature of the predominant anion in the incubation medium. 2. With chloride, bromide or iodide as the predominant anion, ATP synthesis linked to the oxidation of pyruvate or succinate and ATP hydrolysis stimulated by 2,4-dinitrophenol are much more sensitive to triethyltin than they are when nitrate or isethionate is the predominant anion. 3. When nitrate or isethionate is the predominant anion, oxygen uptake stimulated by 2,4-dinitrophenol is not inhibited by triethyltin. 4. In the presence of nitrate or isethionate anions, inhibition of ATP synthesis is directly related to the binding of triethyltin to mitochondria. 5. The relationship of the above effects to the anion-hydroxide ion exchange mediated by triethyltin and the relevance of this to published arrangements for coupling of electron transport to ATP synthesis are discussed.
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D001665:Binding Sites; D001965:Bromides / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology; D004579:Electron Transport; D066298:In Vitro Techniques; D007454:Iodides / Q000494:pharmacology; D007477:Ions / Q000494:pharmacology*; D008928:Mitochondria / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects*; D009566:Nitrates / Q000494:pharmacology; D009942:Organometallic Compounds / Q000037:antagonists & inhibitors / Q000378:metabolism; D010084:Oxidation-Reduction; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D011773:Pyruvates / Q000378:metabolism; D051381:Rats; D013451:Sulfonic Acids / Q000494:pharmacology; D014001:Tin / Q000037:antagonists & inhibitors / Q000378:metabolism
D001965:Bromides; D002712:Chlorides; D004140:Dinitrophenols; D007454:Iodides; D007477:Ions; D009566:Nitrates; D009942:Organometallic Compounds; D011773:Pyruvates; D013451:Sulfonic Acids; D014001:Tin; D000255:Adenosine Triphosphate