Self-renaturing fractions in the separated strands of mouse satellite deoxyribonucleic acid.
Pyrimidine- and purine-rich strands of Mus musculus satellite DNA prepared by alkaline CsCl-gradient centrifugation can self-renature to a variable extent to give partial duplexes with high thermal stability. These duplexes were purified by treatment with nuclease S(1) followed by hydroxylapatite chromatography, and have been shown by pyrimidine-tract analysis to be very similar in sequence to total reassociated satellite DNA. It is believed that the self-renaturing fractions result from variable contamination of each strand with fragments of the other, rather than from molecular inversions. The predominantly single-stranded properties of these fractions may be partly due to the ability of mouse satellite DNA strands to reassociate in non-stoicheiometric proportions.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002845:Chromatography; D004247:DNA / Q000032:analysis; D004277:DNA, Single-Stranded; D003851:Deoxyribonucleases; D051379:Mice; D009691:Nucleic Acid Denaturation; D010759:Phosphorus Isotopes; D011687:Purines / Q000032:analysis*; D011743:Pyrimidines / Q000032:analysis*
D004277:DNA, Single-Stranded; D010759:Phosphorus Isotopes; D011687:Purines; D011743:Pyrimidines; D004247:DNA; D003851:Deoxyribonucleases