Kinetics of the reversible reaction of papain with 5,5'-dithiobis-(2-nitrobenzoate) dianion: evidence for nucleophilic reactivity in the un-ionized thiol group of cysteine-25 and for general acid catalysis by histidine-159 of the reaction of the 5-mercapto-2-nitrobenzoate dianion with the papain-5-mercapto-2-nitrobenzoate mixed disulphide.
D001565:Benzoates; D002384:Catalysis; D003545:Cysteine; D004220:Disulfides; D006639:Histidine; D007700:Kinetics; D009574:Nitro Compounds; D010206:Papain; D013438:Sulfhydryl Compounds; D013440:Sulfides
D001565:Benzoates; D004220:Disulfides; D009574:Nitro Compounds; D013438:Sulfhydryl Compounds; D013440:Sulfides; D006639:Histidine; D010206:Papain; D003545:Cysteine