title: Molecular properties of lupin and serradella leghaemoglobins.
abstract: 1. Leghaemoglobins were extracted from the root nodules of lupin (Lupinus luteus L.) and serradella (Ornithopus sativus Brot.) plants and fractionated into different leghaemoglobin components on DEAE-cellulose-acetate columns. 2. The first two fractions eluted from columns loaded with either lupin or serradella leghaemoglobins were in the Fe(3+) oxidation state. 3. These components have protohaem IX as the prosthetic group and glycine as the N-terminal amino acid. 4. Other properties are: lupin component I, pI5.08, molecular weight 19000; lupin component II, pI5.13, molecular weight 20600; serradella component I, pI5.00, molecular weight 17500; serradella component II, pI5.05, molecular weight 19100. 5. Leghaemoglobins are thus heterogeneous with respect to size and charge.
mesh_terms: D000595:Amino Acid Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D002848:Chromatography, DEAE-Cellulose; D005998:Glycine; D006420:Hemeproteins; D007501:Iron; D007525:Isoelectric Focusing; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010940:Plant Proteins
chemical_list: D006420:Hemeproteins; D010940:Plant Proteins; D007501:Iron; D005998:Glycine