Microbial metabolism of C 1  and C 2  compounds. The role of glyoxylate, glycollate and acetate in the growth of Pseudomonas AM1 on ethanol and on C 1  compounds.
Succinate (or a product of succinate metabolism) is a catabolite repressor of some enzymes of the serine pathway (hydroxypyruvate reductase, serine-glyoxylate aminotransferase and glycerate kinase) but not of methanol dehydrogenase nor methylamine dehydrogenase. A mutant (PCT64) of Pseudomonas AM1, which is unable to grow on C(1) compounds, lacks glycerate kinase, showing that this enzyme is essential for the operation of the serine pathway. Mutant PCT48, unable to convert acetate into glycollate, has lost the ability to grow both on C(1) compounds and on ethanol. The properties of a third mutant (PCT57) show that Pseudomonas AM1 contains enzymes catalysing the conversion of acetate into glyoxylate. Evidence is presented that hydroxypyruvate reductase is involved in the oxidation of glycollate to glyoxylate during growth on ethanol. A scheme is proposed for the conversion of ethanol and of C(1) compounds into glyoxylate in which acetate (or a derivative) and glycollate are intermediates.
D000085:Acetates / Q000378:metabolism*; D000429:Alcohol Oxidoreductases; D002247:Carbon Isotopes; D000431:Ethanol / Q000378:metabolism*; D006016:Glycolates / Q000378:metabolism*; D006038:Glyoxylates / Q000378:metabolism*; D009154:Mutation; D010101:Oxygen Consumption; D010770:Phosphotransferases; D011549:Pseudomonas / Q000254:growth & development*; D011773:Pyruvates; D012694:Serine; D013386:Succinates
D000085:Acetates; D002247:Carbon Isotopes; D006016:Glycolates; D006038:Glyoxylates; D011773:Pyruvates; D013386:Succinates; D000431:Ethanol; D012694:Serine; D000429:Alcohol Oxidoreductases; D010770:Phosphotransferases