Glutathione synthesis in human erythrocytes. II. Purification and properties of the enzymes of glutathione biosynthesis.
The two enzymes required to synthesize glutathione de novo have been purified from human erythrocytes. Glutamylcysteine synthetase was purified 4300-fold and was approximately 80% pure based on polyacrylamide gel electrophoresis. The purified enzyme catalyzes the formation of 30.5 mumoles of gamma-glutamyl-cysteine per mg of protein per hr and is inhibited by sulfhydryl inhibitors. Glutathione synthetase was purified 6000-fold from erythrocytes to homogeneity as determined by polyacrylamide gel electrophoresis. The erythrocyte enzyme has a molecular weight of 150,000 and catalyzes the formation of 35.9 mumoles of glutathione per mg of protein per hr. Comparison of the amino acid composition and some kinetic parameters of yeast glutathione synthetase and the erythrocyte enzyme demonstrate similarities between these enzymes.
D000081:Acetamides / Q000494:pharmacology; D000596:Amino Acids / Q000032:analysis; D000963:Antimetabolites / Q000494:pharmacology; D001681:Biological Assay; D002247:Carbon Isotopes; D002384:Catalysis; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002845:Chromatography; D002848:Chromatography, DEAE-Cellulose; D003545:Cysteine / Q000378:metabolism; D004589:Electrophoresis, Disc; D004912:Erythrocytes / Q000378:metabolism*; D005033:Ethylmaleimide / Q000494:pharmacology; D005971:Glutamates / Q000378:metabolism; D005978:Glutathione / Q000096:biosynthesis*; D005998:Glycine / Q000378:metabolism; D006801:Humans; D008025:Ligases / Q000032:analysis / Q000302:isolation & purification*; D013438:Sulfhydryl Compounds / Q000037:antagonists & inhibitors; D014316:Tritium; D014461:Ultracentrifugation
D000081:Acetamides; D000596:Amino Acids; D000963:Antimetabolites; D002247:Carbon Isotopes; D005971:Glutamates; D013438:Sulfhydryl Compounds; D014316:Tritium; D008025:Ligases; D005978:Glutathione; D003545:Cysteine; D005033:Ethylmaleimide; D005998:Glycine