title: The molecular weights of two forms of carbamoyl phosphate synthase from rat liver.
abstract: 1. N-Acetylglutamate-dependent carbamoyl phosphate synthase from rat liver was centrifuged in sucrose density gradients. The concentration-dependence of s was consistent with a chemical equilibrium existing between the 11S and 7.5S forms of the enzyme. 2. Under conditions favouring the 11S form, the properties of the enzyme in ultra-short-column equilibrium experiments suggest a molecular weight of 316000+/-42000 for the 11S form. 3. Under conditions favouring the 7.5S form, high-speed equilibrium-sedimentation measurements gave a value of 160000+/-10000 as the molecular weight of the 7.5S form of the enzyme.
mesh_terms: D000818:Animals; D002219:Carbamates; D002499:Centrifugation, Density Gradient; D002848:Chromatography, DEAE-Cellulose; D007527:Isoenzymes; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D010770:Phosphotransferases / Q000302:isolation & purification; D051381:Rats
chemical_list: D002219:Carbamates; D007527:Isoenzymes; D010770:Phosphotransferases