NH 2 -terminal residues of Neurospora crassa proteins.
The NH(2)-terminal amino acid composition of the soluble and ribosomal proteins from Neurospora crassa mycelia and conidia was determined by the dinitrophenyl method. A nonrandom distribution of NH(2)-terminal amino acids was observed in the complex protein mixtures. Glycine, alanine, and serine accounted for 75% of the NH(2)-terminal amino acids, and glycine appeared most frequently in mature proteins of mycelia. The appearance of phenylalanine as one of the major NH(2)-termini in crude conidial fraction suggests that the composition of proteins may vary in different developmental stages.
D000409:Alanine / Q000032:analysis; D000596:Amino Acids / Q000032:analysis*; D001322:Autoanalysis; D002474:Cell-Free System; D002854:Chromatography, Paper; D003470:Culture Media; D004140:Dinitrophenols; D005998:Glycine / Q000032:analysis; D006868:Hydrolysis; D009491:Neurospora / Q000032:analysis* / Q000145:classification / Q000254:growth & development; D010649:Phenylalanine / Q000032:analysis; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification; D012694:Serine / Q000032:analysis; D013053:Spectrophotometry
D000596:Amino Acids; D003470:Culture Media; D004140:Dinitrophenols; D011506:Proteins; D012694:Serine; D010649:Phenylalanine; D000409:Alanine; D005998:Glycine