Morphological responses of prostatic carcinoma to testosterone in organ culture.
Slices of human prostatic adenocarcinoma obtained by transurethral resection were maintained in organ culture for 4 days. Preservation of histological appearance was good with little evidence of necrosis within the viable tissue. Slices of tumour cultured in the presence of testosterone showed a morphological change to a more differentiated type of neoplasm whereas explants cultured in the absence of steroid hormone, or with stilboestrol diphosphate, showed no change. In the case of a relatively anaplastic tumour, testosterone produced a significant increase in the number of mitotic figures seen.
D000230:Adenocarcinoma / Q000188:drug therapy; D000368:Aged; D002454:Cell Differentiation / Q000187:drug effects; D004054:Diethylstilbestrol / Q000627:therapeutic use; D006801:Humans; D008297:Male; D008875:Middle Aged; D008938:Mitosis / Q000187:drug effects; D009924:Organ Culture Techniques; D011471:Prostatic Neoplasms / Q000188:drug therapy*; D013739:Testosterone / Q000627:therapeutic use*
D013739:Testosterone; D004054:Diethylstilbestrol