Nucleic acid enzymology of extremely halophilic bacteria. Gel-filtration and density-gradient-centrifugation studies of the molecular weights of Halobacterium cutirubrum polynucleotide phosphorylase and deoxyribonucleic acid- and ribonucleic acid-dependent ribonucleic acid polymerases.
1. Conditions have been established for the estimation of molecular weights of proteins by analytical gel filtration and sucrose-density-gradient centrifugation in 2.5m-potassium chloride-1m-sodium chloride; Halobacterium cutirubrum polynucleotide phosphorylase, DNA-dependent RNA polymerase and RNA-dependent RNA polymerase have been studied by these methods. 2. The RNA-dependent polymerase has also been studied by density-gradient centrifugation in the absence of salt. 3. All three proteins are of unusually low molecular weight compared with similar enzymes from non-halophilic bacteria.
D000178:Acrylamides; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D003580:Cytochromes / Q000032:analysis; D004247:DNA; D003911:Dextrans; D006217:Halobacterium / Q000201:enzymology*; D008722:Methods; D008970:Molecular Weight; D011189:Potassium Chloride; D011506:Proteins / Q000032:analysis; D012313:RNA; D012316:RNA Nucleotidyltransferases; D012965:Sodium Chloride; D013053:Spectrophotometry; D014461:Ultracentrifugation
D000178:Acrylamides; D003580:Cytochromes; D003911:Dextrans; D011506:Proteins; D012965:Sodium Chloride; D012313:RNA; D011189:Potassium Chloride; D004247:DNA; D012316:RNA Nucleotidyltransferases