Ascorbic acid: a culture requirement for colony formation by mouse plasmacytoma cells.
Plasmacytoma stem cells explanted from mice formed colonies in culture only in the presence of L-ascorbic acid; this vitamin was not needed for the formation of granulocytic colonies. Isomers of L-ascorbic acid with less antiscorbutic activity also promoted plasmacytoma colony formation, but less effectively. Other redox compounds without antiscorbutic activity and an antioxidant were ineffective.
D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism*; D001854:Bone Marrow Cells; D002478:Cells, Cultured / Q000378:metabolism*; D003470:Culture Media; D007536:Isomerism; D051379:Mice; D010954:Plasmacytoma; D013154:Spleen
D003470:Culture Media; D001205:Ascorbic Acid