Substitution of 4-(2-hydroxyethyl)-1-piperazineethane sulfonic acid (HEPES) for bicarbonate in protein-free animal cell culture medium: application to vaccinia virus quantitation and fluorogenic acetylesterase assay in living LM cells.
D000115:Acetylesterase / Q000378:metabolism*; D000818:Animals; D001639:Bicarbonates; D002021:Buffers; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology*; D003238:Connective Tissue; D003470:Culture Media; D046508:Culture Techniques / Q000592:standards; D005470:Fluorometry; D051379:Mice; D010100:Oxygen; D010879:Piperazines / Q000494:pharmacology; D013451:Sulfonic Acids / Q000494:pharmacology; D014616:Vaccinia virus / Q000254:growth & development* / Q000472:pathogenicity
D001639:Bicarbonates; D002021:Buffers; D003470:Culture Media; D010879:Piperazines; D013451:Sulfonic Acids; D000115:Acetylesterase; D010100:Oxygen