Ultrafiltration as a method for concentrating Rift Valley fever virus grown in tissue culture.
Filtration by means of a Diaflo ultrafilter was used to concentrate three 1,000-ml lots and one 3,000-ml lot of tissue culture-grown Rift Valley fever virus. Quantitation of both infectivity and total protein was achieved. Water treatment with continued ultrafiltration of the virus concentrate provided a final virus product approximately 99.25% free of low-molecular-weight materials originally present in the growth medium.
D000818:Animals; D001103:Arboviruses / Q000254:growth & development / Q000302:isolation & purification* / Q000472:pathogenicity; D046508:Culture Techniques; D003956:Dialysis; D005374:Filtration; D008722:Methods; D012295:Rift Valley Fever; D013696:Temperature; D014764:Viral Proteins / Q000302:isolation & purification; D014776:Virus Cultivation; D014867:Water
D014764:Viral Proteins; D014867:Water