Formation of 1-O-2'-hydroxyalkyl glycerophosphatides from 1,2-heptadecanediol in myelinating brain.
1,2-Heptadecanediol-2-(14)C was administered intracerebrally to 18-day-old rats, and its incorporation, after 8 hr, into the individual aliphatic moieties of the ethanolamine glycerophosphatides was determined. Much of the radioactivity was found in a lipid fraction identified as 1-O-2'-hydroxyheptadecyl glycerol. Evidence is presented that a major portion of the precursor was incorporated into 1-O-2'-hydroxyheptadecyl-2-acyl ethanolamine phosphatides. Some of the diol administered was degraded to palmitic acid. The palmitic acid-1-(14)C derived from 1,2-heptadecanediol-2-(14)C apparently served as precursor for stearic and oleic acids, which were found as acyl groups, and for the biosynthesis of the corresponding O-alkyl and O-alk-1-enyl glycerols. The data presented prove that biological dehydration of 1-O-2'-hydroxyalkyl glycerophosphatides to the corresponding plasmalogens does not occur in myelinating brain.
D000215:Acylation; D000438:Alcohols / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D001923:Brain Chemistry; D002247:Carbon Isotopes; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D004988:Ethers, Cyclic / Q000032:analysis; D005990:Glycerol / Q000032:analysis; D006868:Hydrolysis; D007259:Infrared Rays; D008055:Lipids / Q000032:analysis; D009186:Myelin Sheath / Q000378:metabolism; D010169:Palmitic Acids; D010714:Phosphatidylethanolamines / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D013057:Spectrum Analysis
D000438:Alcohols; D002247:Carbon Isotopes; D004988:Ethers, Cyclic; D008055:Lipids; D010169:Palmitic Acids; D010714:Phosphatidylethanolamines; D005990:Glycerol