title: Receptors on immunocompetent cells. 3. Specificity and nature of receptors on dinitrophenylated guinea pig albumin- 125 I-binding cells of immunized guinea pigs.
abstract: Guinea pigs immunized with 2,4-dinitrophenyl-guinea pig albumin (DNP-GPA) possess lymphocytes which specifically bind sufficient DNP-GPA-(125)I to their surface to be detected by radioautography. These lymphocytes are present in the draining lymph nodes in a frequency of approximately 50/1000 lymphocytes in animals immunized 2-4 wk earlier with DNP-GPA in complete Freund's adjuvant. Nonimmunized animals have approximately 0.4 DNP-GPA antigen-binding cells (ABC) per 1000 lymphocytes. An increase in the frequency of DNP-GPA ABC in peripheral blood is detectable by 5 days after immunization, which is before the time that serum anti-DNP antibody is measurable. The receptors of these ABC are hapten specific in that free epsilon-DNP-L-lysine, at low concentration, inhibits the binding of DNP-GPA-(125)I; DNP bovine serum alumbin (DNP-BSA) is equivalent to DNP-GPA in the inhibition of binding of DNP-GPA-(125)I to ABC; and both DNP-GPA agarose beads and DNP-BSA agarose beads specifically adsorb DNP-GPA-(125)I ABC. Anti-immunoglobulin antisera, particularly anti-gamma(2) sera, inhibit the binding of DNP-GPA-(125)I to these cells implying that the receptors are immunoglobulin, primarily of the gamma(2) heavy chain class. DNP-GPA-(125)I ABC appear to represent precursors of antibody-secreting cells and have specificity characteristics which are very different from cells, of similarly immunized guinea pigs, which mediate a cellular immune response to DNP-GPA.
mesh_terms: D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D000921:Antibody-Producing Cells; D000941:Antigens; D001345:Autoradiography; D001665:Binding Sites; D004140:Dinitrophenols; D006041:Goats; D006168:Guinea Pigs; D006241:Haptens; D007111:Immunity, Cellular; D007114:Immunization; D008214:Lymphocytes / Q000276:immunology*; D011817:Rabbits; D012711:Serum Albumin, Radio-Iodinated
chemical_list: D000906:Antibodies; D000941:Antigens; D004140:Dinitrophenols; D006241:Haptens; D012711:Serum Albumin, Radio-Iodinated