Rapid methods for determining decarboxylase activity: arginine decarboxylase.
A rapid biochemical method for the determination of arginine decarboxylase (EC 4.1.1.19) activity has been developed for use in the routine clinical microbiology laboratory and correlated with similar procedures for ornithine and lysine decarboxylase (EC 4.1.1.18) systems. It is based on the detection of agmatine, the amine end product formed during growth on a synthetic medium containing arginine as the key amino acid. A modified diacetyl reagent is used to detect this amine after a differential butanol extraction of the cultures. This procedure can be used to detect this amine after a 1- to 4-hr incubation period (with the use of an initial concentrated inoculum) or with an overnight culture. Thus, both an indirect measurement based on the alkalinization of the medium and a lengthy incubation period were avoided. Parameters for optimal enzyme activity and the pertinent enzyme systems involved in arginine and agmatine catabolism are discussed in detail.
D000438:Alcohols; D000588:Amines / Q000032:analysis / Q000096:biosynthesis; D001119:Arginase / Q000378:metabolism; D001120:Arginine / Q000378:metabolism; D001419:Bacteria / Q000145:classification / Q000201:enzymology* / Q000378:metabolism; D002262:Carboxy-Lyases / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D003470:Culture Media; D003653:Decarboxylation; D004755:Enterobacteriaceae / Q000145:classification / Q000201:enzymology / Q000378:metabolism; D005069:Evaluation Studies as Topic; D006146:Guanidines / Q000032:analysis / Q000096:biosynthesis; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000378:metabolism; D007202:Indicators and Reagents; D008722:Methods; D010100:Oxygen; D013053:Spectrophotometry; D013696:Temperature
D000438:Alcohols; D000588:Amines; D003470:Culture Media; D006146:Guanidines; D007202:Indicators and Reagents; D001120:Arginine; D006867:Hydrolases; D001119:Arginase; D002262:Carboxy-Lyases; D010100:Oxygen