title: An evaluation of four methods for measuring cholesterol absorption by the intestine in man.
abstract: Critical comparisons have been made in 12 patients of four methods for measuring cholesterol absorption from the intestine. Methods I-III depend on the use of labeled cholesterol (intravenously or continuous labeling orally) in conjunction with sterol balance measurements; Method IV can be carried out with only a single test dose containing labeled cholesterol plus labeled beta-sitosterol. In the latter technique absorption is calculated as the loss of cholesterol relative to beta-sitosterol during intestinal transit. Method III (isotopic steady-state method) proved to be undependable because of uncertainties in determining the existence of an isotopic steady state. However, Method IV gave good agreement with Methods I and II, and it appears to have certain practical as well as theoretical advantages. Although Method IV requires collections of stools for up to 8 days, it is nevertheless the most rapid and the simplest of all the methods for estimating absorption. It can also be used in certain situations, such as in fur-licking animals, when Methods I and II are inadequate. Therefore, this method would seem to be a valuable addition to other isotopic techniques for estimating cholesterol absorption in man.
mesh_terms: D000012:Abetalipoproteinemia / Q000378:metabolism; D000284:Administration, Oral; D000328:Adult; D000368:Aged; D000818:Animals; D001827:Body Height; D001835:Body Weight; D002247:Carbon Isotopes; D002648:Child; D002784:Cholesterol / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D003369:Cottonseed Oil; D004041:Dietary Fats; D005243:Feces / Q000032:analysis; D005260:Female; D005989:Glycerides / Q000097:blood; D006120:Grooming; D006197:Hair; D006706:Homeostasis; D006801:Humans; D006937:Hypercholesterolemia / Q000378:metabolism; D006949:Hyperlipidemias / Q000378:metabolism; D007223:Infant; D007275:Injections, Intravenous; D007408:Intestinal Absorption; D007700:Kinetics; D008103:Liver Cirrhosis / Q000378:metabolism; D008297:Male; D008722:Methods; D008875:Middle Aged; D009821:Oils; D013256:Steroids / Q000378:metabolism; D013261:Sterols / Q000378:metabolism; D013997:Time Factors; D014316:Tritium; D014973:Xanthomatosis / Q000378:metabolism; D003313:Zea mays
chemical_list: D002247:Carbon Isotopes; D003369:Cottonseed Oil; D004041:Dietary Fats; D005989:Glycerides; D009821:Oils; D013256:Steroids; D013261:Sterols; D014316:Tritium; D002784:Cholesterol