title: Pigment production of Cryptococcus neoformans grown with extracts of Guizotia abyssinica.
abstract: Brown pigment(s) formed in Cryptococcus neoformans when grown on media containing extracts of the seeds of Guizotia abyssinica cannot be extracted by common organic solvents or by 6 n HCl or 2 n NaOH. A similar pigmentation was observed in C. neoformans when grown on a medium containing caffeic acid isolated from the hydrolyzed methanol extract of G. abyssinica seeds. Its methyl ester and the diacetate thereof, as well as the following structurally related compounds, 3-hydroxytyramine, 3,4-dihydroxybenzoic acid, 3,4-dihydroxyphenylethanolamine, and 4-hydroxy-3,5-dimethoxycinnamic acid, brought about similar pigmentation. However, 2,4-, 2,5-, 2,6-, and 3,5-dihydroxybenzoic acids, tyrosine, phenylalanine, cinnamic acid, 4-hydroxycinnamic acid, and 4-hydroxy-3-methoxycinnamic acid did not cause coloration in C. neoformans.
mesh_terms: D002934:Cinnamates / Q000032:analysis; D003454:Cryptococcus / Q000378:metabolism*; D003470:Culture Media; D007259:Infrared Rays; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010860:Pigments, Biological / Q000096:biosynthesis*; D010936:Plant Extracts / Q000032:analysis; D010944:Plants; D012639:Seeds; D012997:Solvents; D013045:Species Specificity; D013057:Spectrum Analysis
chemical_list: D002934:Cinnamates; D003470:Culture Media; D010860:Pigments, Biological; D010936:Plant Extracts; D012997:Solvents