Evidence for the presence of a pool of glycerides with a rapid rate of turnover in brown fat from newborn rabbits.
1. The specific radioactivity of [(14)C]glycerol released during the incubation of brown fat with [(14)C]glucose is much greater than that of the tissue lipid glycerol. 2. From a study of the release of [(14)C]glycerol from pre-labelled brown fat, it is concluded that the tissue contains a pool of glycerides with a higher rate of turnover than those in the main lipid store. 3. This pool contains newly synthesized glycerides, has a half-life of 25-30min and supplies about 25% of the glycerol liberated by brown fat. 4. Thus, a significant fraction of the total (14)C incorporated from glucose into brown-fat lipids is released as [(14)C]glycerol during an incubation.
D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D002247:Carbon Isotopes; D005947:Glucose / Q000378:metabolism; D005989:Glycerides / Q000096:biosynthesis / Q000378:metabolism*; D005990:Glycerol / Q000096:biosynthesis; D006207:Half-Life; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D011817:Rabbits; D014280:Triglycerides / Q000096:biosynthesis; D014316:Tritium
D002247:Carbon Isotopes; D005989:Glycerides; D014280:Triglycerides; D014316:Tritium; D005947:Glucose; D005990:Glycerol