Purification of ribonuclease T 1  by diethylaminoethylcellulose chromatography.
A procedure is described for isolating the enzyme ribonuclease T(1) from Takadiastase, an extract of the mould Aspergillus oryzae. It involves an initial concentration of the enzyme by adsorption on DEAE-cellulose followed by gradient elution. Later the enzyme is chromatographed on the same adsorbent with an eluent of constant composition. Yields of 350-380mg of ribonuclease T(1) from 500g of Takadiastase were obtained.
D000681:Amylases / Q000032:analysis; D001230:Aspergillus / Q000201:enzymology; D002848:Chromatography, DEAE-Cellulose; D004586:Electrophoresis; D006863:Hydrogen-Ion Concentration; D008722:Methods; D012260:Ribonucleases / Q000302:isolation & purification*
D012260:Ribonucleases; D000681:Amylases