Deoxyribonucleic acid synthesis in ultraviolet-light-irradiated Chinese hamster cells.
Two mammalian cell lines, Chinese hamster ovary (CHO) which can recover colony-forming ability between fractionated doses of ultraviolet light (UV), and Chinese hamster B-14FAF28 which cannot recover, were tested for the ability to bypass UV-induced photoproducts in DNA during postirradiation DNA synthesis. The molecular weight distributions of newly synthesized DNA in UV-irradiated populations of both cell lines showed evidence for photoproduct bypass. Hence, the bypass mechanism does not correlate with recovery after UV.
D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D004247:DNA / Q000096:biosynthesis*; D005260:Female; D008970:Molecular Weight; D010053:Ovary / Q000378:metabolism / Q000528:radiation effects*; D011830:Radiation Effects; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium; D014466:Ultraviolet Rays
D014316:Tritium; D004247:DNA; D013936:Thymidine