The cellulase of Fusarium solani. Purification and specificity of the  -(1-4)-glucanase and the  -D-glucosidase components.
1. Cell-free culture filtrates of the fungus Fusarium solani were examined for homogeneity with respect to beta-d-glucosidase and C(x) activities. 2. o-Nitrophenyl beta-d-glucoside and cellobiose were both used as substrates for beta-d-glucosidase activity. 3. No evidence for the non-identity of nitrophenyl beta-d-glucosidase and cellobiase activities could be found, either by heat treatment, gel filtration on Sephadex G-100 or by isoelectric focusing. 4. The beta-d-glucosidase component was also a feeble exo-beta-glucanase: it had a molecular weight of approx. 400000. 5. The fall in viscosity of a solution of CM-cellulose, the formation of reducing sugars in a solution of CM-cellulose and the solubilization of phosphoric acid-swollen cellulose (Walseth cellulose), were all used for the measurement of C(x) activity. 6. The ratio of the two types of CM-cellulase activity was not changed after gel filtration on Sephadex G-100 or after chromatography on DEAE-Sephadex. 7. Three peaks of C(x) activity were obtained after electrofocusing, but all three possessed the same ratio of the two types of CM-cellulase activity as well as the same CM-cellulase/Walseth activity ratio, as the unfractionated enzyme; all three isoenzymes (isoelectric points, 4.75, 4.80-4.85 and 5.15) acted in synergism with a mixture of the C(1) and the beta-d-glucosidase components to the same extent in the solubilization of cotton fibre. 8. The molecular weight of the C(x) component was approx. 37000.
D002482:Cellulose; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D003911:Dextrans; D005670:Fusarium / Q000201:enzymology*; D005959:Glucosidases / Q000302:isolation & purification*; D006026:Glycoside Hydrolases / Q000302:isolation & purification*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007783:Lactones / Q000037:antagonists & inhibitors; D008970:Molecular Weight; D009596:Nitrophenols / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D014783:Viscosity
D003911:Dextrans; D007783:Lactones; D009596:Nitrophenols; D011506:Proteins; D002482:Cellulose; D005959:Glucosidases; D006026:Glycoside Hydrolases