Studies on gastric mucoproteins. The isolation and characterization of the mucoprotein of the water-soluble mucus from pig cardiac gastric mucosa.
1. Gel filtration of the water-soluble radioactive mucus produced three radioactive fractions, fraction A excluded on Sepharose 4B, fraction B included on Sepharose 4B but excluded on Sephadex G-200, and fraction C included on Sephadex G-200. 2. The specific radioactivities of fractions A and B were the same, with fraction C a little lower, whether the material was labelled with (14)C-labelled carbohydrate or with (3)H-labelled protein prepared by incubation of mucosal scrapings in vitro with [U-(14)C]glucose or [G-(3)H]threonine respectively. 3. Fractions A and B had an analysis of protein 22%, hexose 28%, hexosamine 28%, fucose 10% and sialic acid 1%; fraction C had an analysis closely similar to this, except that it contained about 10% of a protein contaminant. 4. All three fractions had closely similar A and H blood-group activities. 5. Ultracentrifuge studies showed fractions A, B and C were polydisperse with s(0) (25,w) values of 18.7S, 4.9S and 3.9S respectively. 6. The unfractionated water-soluble mucus contained only two peaks, fraction A 18.7S and a peak of 4.4S, which was a combination of fractions B and C. 7. The radioactive mucoprotein accounted for 85% by weight of the soluble mucus and the results show that it consisted of two distinct fractions A and B-C, which were chemically, biosynthetically and immunologically very similar.
D000017:ABO Blood-Group System / Q000032:analysis; D000818:Animals; D001789:Blood Group Antigens; D002247:Carbon Isotopes; D002299:Cardia / Q000032:analysis; D002850:Chromatography, Gel; D005643:Fucose / Q000032:analysis; D005753:Gastric Mucosa / Q000032:analysis* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006385:Hemagglutination Inhibition Tests; D006595:Hexosamines / Q000032:analysis; D006601:Hexoses / Q000032:analysis; D009077:Mucins / Q000302:isolation & purification; D009088:Mucoproteins / Q000032:analysis / Q000302:isolation & purification*; D009093:Mucus / Q000032:analysis*; D009438:Neuraminic Acids / Q000032:analysis; D013552:Swine; D013912:Threonine / Q000378:metabolism; D014316:Tritium; D014461:Ultracentrifugation
D000017:ABO Blood-Group System; D001789:Blood Group Antigens; D002247:Carbon Isotopes; D006595:Hexosamines; D006601:Hexoses; D009077:Mucins; D009088:Mucoproteins; D009438:Neuraminic Acids; D014316:Tritium; D005643:Fucose; D013912:Threonine; D005947:Glucose