title: The transport of citrate and other tricarboxylic acids in two species of Pseudomonas.
abstract: When cells of Pseudomonas are grown on citrate as the sole carbon source they oxidize citrate and isocitrate rapidly. Fluorocitrate inhibits the oxidation of citrate. Fluorocitrate-treated cells accumulate [6-(14)C]citrate, as shown by a rapid Millipore-filtration technique. In the absence of fluorocitrate most of the [6-(14)C]-citrate is lost in the form of (14)CO(2). The isolation of a pseudomonad characterized by its ability to grow on tricarballylate as a sole carbon source has facilitated the study of the tricarboxylate-carrier specificity. Cells grown on citrate will exchange radioactive citrate for unlabelled citrate or isocitrate but not for cis-aconitate, trans-aconitate or tricarballylate. Cells grown on tricarballylate will exchange radioactive citrate for unlabelled citrate, cis-aconitate or tricarballylate, but not for isocitrate or trans-aconitate. The properties of the exchange system involved are compared with those of the related system in mitochondria.
mesh_terms: D000498:Allyl Compounds / Q000378:metabolism; D002245:Carbon Dioxide / Q000096:biosynthesis; D002247:Carbon Isotopes; D002951:Citrates / Q000096:biosynthesis / Q000378:metabolism*; D005461:Fluorine; D007523:Isocitrates / Q000378:metabolism; D010084:Oxidation-Reduction; D011549:Pseudomonas / Q000254:growth & development / Q000378:metabolism*; D013045:Species Specificity; D014233:Tricarboxylic Acids / Q000378:metabolism*
chemical_list: D000498:Allyl Compounds; D002247:Carbon Isotopes; D002951:Citrates; D007523:Isocitrates; D014233:Tricarboxylic Acids; D002245:Carbon Dioxide; D005461:Fluorine