Bacterial metabolism of 4-chloro-2-methylphenoxyacetate. Formation of glyoxylate by side-chain cleavage.
Crude extracts of Pseudomonas sp. grown on 4-chloro-2-methylphenoxyacetate as sole source of carbon were shown to oxidize 4-chloro-2-methylphenoxyacetate to 5-chloro-o-cresol and glyoxylate. A labelled 2,4-dinitrophenylhydrazone was isolated from an incubation mixture in which 4-chloro-2-methylphenoxy[carboxy-(14)C]acetate was used. The hydrazone was shown to behave identically on thin-layer chromatograms with the authentic 2,4-dinitrophenylhydrazone of glyoxylate. Radioactivity assay showed that 82% of the side chain of 4-chloro-2-methylphenoxyacetate was recovered as glyoxylate.
D000085:Acetates / Q000378:metabolism; D001345:Autoradiography; D002247:Carbon Isotopes; D002855:Chromatography, Thin Layer; D003408:Cresols / Q000378:metabolism; D003470:Culture Media; D006016:Glycolates / Q000378:metabolism*; D006038:Glyoxylates / Q000378:metabolism; D006540:Herbicides / Q000378:metabolism*; D006835:Hydrazones; D006863:Hydrogen-Ion Concentration; D009578:Nitrobenzenes; D010084:Oxidation-Reduction; D011549:Pseudomonas / Q000378:metabolism*; D013053:Spectrophotometry; D014732:Vibration
C001072:2,4-dinitrophenylhydrazone; D000085:Acetates; D002247:Carbon Isotopes; D003408:Cresols; D003470:Culture Media; D006016:Glycolates; D006038:Glyoxylates; D006540:Herbicides; D006835:Hydrazones; D009578:Nitrobenzenes