Effects of N-hydroxyurethan on viability and metabolism of Escherichia coli.
Exposure of growing cells to low levels of N-hydroxyurethan (approximately 0.05 m) resulted in loss of colony-forming ability. This was accompanied by degradation of cellular deoxyribonucleic acid. When the concentration of N-hydroxyurethan was increased to 0.2 m, bacteriostasis was the primary manifestation. The lethal action of the drug was prevented by contreatment with chloramphenicol or azauracil or by uracil-deprivation of a bacterial strain requiring this pyrimidine for growth.
D000890:Anti-Infective Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000096:biosynthesis; D002219:Carbamates / Q000494:pharmacology; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002701:Chloramphenicol / Q000494:pharmacology; D002712:Chlorides; D002852:Chromatography, Ion Exchange; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis / Q000096:biosynthesis; D014498:Uracil / Q000031:analogs & derivatives / Q000494:pharmacology
D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D002219:Carbamates; D002247:Carbon Isotopes; D002712:Chlorides; D003470:Culture Media; D004269:DNA, Bacterial; D002586:Cesium; D014498:Uracil; D002701:Chloramphenicol