title: Micellar properties of sodium fusidate, a steroid antibiotic structurally resembling the bile salts.
abstract: The properties of sodium fusidate micelles were determined by a spectral shift technique, surface tension measurements, and ultracentrifugal analysis. The critical micellar concentrations, mean molecular areas, and apparent aggregation numbers were estimated as a function of the concentration of counterion (0.001-1.0 m Na(+)) at 20 degrees C. The critical micellar concentrations were studied over a temperature range of 10 degrees C to 40 degrees C at one counterion concentration (0.001 m Na(+)), and from these data the standard thermo-dynamic functions of micellization were calculated. The ability of sodium fusidate solutions to solubilize the insoluble swelling amphiphiles, lecithin and monoolein, was investigated, and the results were compared with the solubilizing properties of sodium taurocholate. The critical micellar concentrations of sodium fusidate approximated those of sodium taurocholate. The values fell in the range of 1.44-4.56 mm, varying with the technique used, counterion concentration, and temperature. The percentage of counterions bound to fusidate micelles in water, calculated from the log critical micellar concentration-log Na(+) curve, was estimated to be negligible, which compares with sodium taurocholate micelles. The critical micellar concentration of sodium fusidate exhibited a minimum at 20 degrees C, a phenomenon observed with other ionic detergents and with bile salts. Micelle formation in sodium fusidate solutions was shown to be primarily entropy-driven at 10 degrees and 20 degrees C, whereas at 30 degrees and 40 degrees C the enthalpy factor predominated. From the surface tension measurements the molecular areas of sodium fusidate and sodium taurocholate were calculated. The mean molecular area of fusidate was 101 A(2), whereas sodium taurocholate possessed a molecular area of 88 A(2). It was demonstrated that the sodium fusidate molecule, like a bile salt molecule, lies with its longitudinal axis horizontal at an air-water interface. The apparent aggregation number of sodium fusidate micelles increased from 5 to 16 as the concentration of counterion increased from 0.01 to 0.60 m Na(+). These values are slightly larger than the corresponding aggregation numbers of sodium taurocholate micelles.
mesh_terms: D001646:Bile; D001647:Bile Acids and Salts; D055598:Chemical Phenomena; D002621:Chemistry; D002769:Cholelithiasis; D003102:Colloids; D005672:Fusidic Acid; D005989:Glycerides; D007536:Isomerism; D008055:Lipids; D046911:Macromolecular Substances; D008433:Mathematics; D008961:Models, Structural; D008970:Molecular Weight; D009994:Osmolar Concentration; D010713:Phosphatidylcholines; D012964:Sodium; D012995:Solubility; D013053:Spectrophotometry; D013500:Surface Tension; D013696:Temperature; D013816:Thermodynamics; D014461:Ultracentrifugation
chemical_list: D001647:Bile Acids and Salts; D003102:Colloids; D005989:Glycerides; D008055:Lipids; D046911:Macromolecular Substances; D010713:Phosphatidylcholines; D005672:Fusidic Acid; D012964:Sodium