Immunochemical detection of minor bases in nucleic acids.
Rabbits immunized with bovine serum albumin conjugates of 5-bromouracil, 5-iodouracil, and 6-methyladenosine produced antibodies specific for the bases. These antibodies were used to detect immunochemically 5-bromouracil and 6-methyladenosine in denatured DNA.
D000241:Adenosine / Q000032:analysis; D000818:Animals; D000906:Antibodies; D001976:Bromouracil / Q000032:analysis; D003168:Complement Fixation Tests; D004247:DNA / Q000032:analysis*; D004269:DNA, Bacterial / Q000032:analysis; D007114:Immunization; D009691:Nucleic Acid Denaturation; D009711:Nucleotides / Q000032:analysis*; D011817:Rabbits; D014498:Uracil / Q000031:analogs & derivatives / Q000032:analysis
D000906:Antibodies; D004269:DNA, Bacterial; D009711:Nucleotides; D001976:Bromouracil; D014498:Uracil; D004247:DNA; D000241:Adenosine