Some properties of mitochondria isolated from the flight muscle of the periodical cicada, Magicicada septendecim.
Mitochondria from the flight muscle of the periodical cicada oxidize pyruvate and d-glycerol 1-phosphate at rates comparable with those obtained with flight-muscle mitochondria from other insects. The oxidation of d-glycerol 1-phosphate is greatly stimulated by low concentrations of Ca(2+). However, oxidative phosphorylation with this substrate is optimum over only a narrow range of Ca(2+) concentration, because of the ability of these mitochondria actively to accumulate Ca(2+) present at micromolar concentrations. The oxidation of pyruvate via the complete tricarboxylic acid cycle is enhanced by high concentrations of phosphate. When both pyruvate and d-glycerol 1-phosphate are present simultaneously, there is no simple summation of the rates obtained with the substrates singly. Acetyl-l-carnitine, palmitoyl-l-carnitine, glutamate and 2-oxoglutarate are oxidized at rates similar to those obtained with mammalian mitochondria, though lower than those obtained with the two prime substrates. However, no other tricarboxylic acid-cycle intermediates added to the medium were oxidized. From these and other observations it has been concluded that these mitochondria possess a previously undescribed combination of substrate-anion permeases.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002331:Carnitine / Q000378:metabolism; D002952:Citric Acid Cycle; D005260:Female; D005971:Glutamates / Q000378:metabolism; D005977:Glutarates / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism; D066298:In Vitro Techniques; D007313:Insecta / Q000378:metabolism*; D008297:Male; D026901:Membrane Transport Proteins / Q000378:metabolism; D008931:Mitochondria, Muscle / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D010539:Permeability; D010710:Phosphates / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism
D005971:Glutamates; D005977:Glutarates; D005994:Glycerophosphates; D026901:Membrane Transport Proteins; D010710:Phosphates; D011773:Pyruvates; D002331:Carnitine; D002118:Calcium