The phospholipid-dependence of uridine diphosphate glucuronyltransferase. Reactivation of phospholipase A-inactivated enzyme by phospholipids and detergents.
Specific degradation of the phospholipid membrane of guinea-pig liver microsomal fraction with phospholipase A inactivated glucuronyltransferase. The inactivation was reversed by phosphatidylcholine and mixed microsomal phospholipid micelles at concentrations similar to those present in intact microsomal preparations. The other commonly occurring phospholipids did not reactivate phospholipase A-treated enzyme. Since the mixed microsomal phospholipids consisted mainly of phosphatidylcholine, it is concluded that the reactivation by phospholipids is phosphatidylcholine-specific. Reactivation was also achieved by low concentrations of the cationic detergents cetylpyridinium chloride and cetyltrimethylammonium bromide. Higher concentrations of these detergents inactivated the glucuronyltransferase activity of intact and phospholipase A-treated microsomal fractions. Anionic detergents were potent inactivators of the glucuronyltransferase activity of untreated and phospholipase A-treated microsomal fractions, whereas non-ionic detergents had little effect on the activity of either preparation. Measurements of the zeta-potentials of the micellar species used in this study showed that no obvious relationship existed between the zeta-potentials and the ability to reactivate glucuronyltransferase. However, high positive or negative zeta-potentials were correlated with the ability of the amphipathic compound to inactivate glucuronyltransferase.
D000818:Animals; D001647:Bile Acids and Salts / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology*; D004586:Electrophoresis; D004789:Enzyme Activation; D006168:Guinea Pigs; D006602:Hexosyltransferases / Q000378:metabolism*; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000201:enzymology*; D010713:Phosphatidylcholines / Q000494:pharmacology; D010740:Phospholipases; D010743:Phospholipids / Q000494:pharmacology*; D011726:Pyridinium Compounds / Q000494:pharmacology; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology
D001647:Bile Acids and Salts; D003902:Detergents; D010713:Phosphatidylcholines; D010743:Phospholipids; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents; D006602:Hexosyltransferases; D010740:Phospholipases