Freezing and melting of lipid bilayers and the mode of action of nonactin, valinomycin, and gramicidin.
An abrupt loss of effectiveness of the presumed carriers, nonactin and valinomycin, in mediating ion conductance occurred at the same temperature as the membrane fluidity, judged visually, was lost. By contrast, the effects of the presumed channel-former, gramicidin, were the same on solid and liquid membranes. Taken together, these findings imply that freezing the membrane primarily reduces the mobility of these antibiotics with little effect on their solubility.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001692:Biological Transport; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005615:Freezing; D006358:Hot Temperature; D008055:Lipids; D008567:Membranes, Artificial; D011189:Potassium Chloride / Q000494:pharmacology; D013997:Time Factors; D014449:Tyrothricin / Q000494:pharmacology*; D014634:Valinomycin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D008055:Lipids; D008567:Membranes, Artificial; D014449:Tyrothricin; D014634:Valinomycin; D011189:Potassium Chloride