Micro method for determination of reactive carbonyl groups in proteins and peptides, using 2,4-dinitrophenylhydrazine.
A method is described for determining carbonyl groups that is especially suitable for use with proteins and peptides. It involves the determination of the extinction at 370nm of a sample solution after adding 2,4-dinitrophenylhydrazine. The reaction of 2,4-dinitrophenylhydrazine with pyruvoylglycine and with transaminated ribonuclease T(1) is presented; the isolation of protein hydrazones is discussed.
D000447:Aldehydes / Q000032:analysis*; D000586:Amination; D000596:Amino Acids / Q000032:analysis; D001322:Autoanalysis; D002848:Chromatography, DEAE-Cellulose; D005998:Glycine; D007659:Ketones / Q000032:analysis*; D008722:Methods; D008832:Microchemistry; D010455:Peptides / Q000032:analysis*; D010659:Phenylhydrazines; D011506:Proteins / Q000032:analysis*; D011773:Pyruvates; D012260:Ribonucleases; D013053:Spectrophotometry; D014466:Ultraviolet Rays
D000447:Aldehydes; D000596:Amino Acids; D007659:Ketones; D010455:Peptides; D010659:Phenylhydrazines; D011506:Proteins; D011773:Pyruvates; D012260:Ribonucleases; D005998:Glycine