The transport of citrate and other tricarboxylic acids in two species of Pseudomonas.
When cells of Pseudomonas are grown on citrate as the sole carbon source they oxidize citrate and isocitrate rapidly. Fluorocitrate inhibits the oxidation of citrate. Fluorocitrate-treated cells accumulate [6-(14)C]citrate, as shown by a rapid Millipore-filtration technique. In the absence of fluorocitrate most of the [6-(14)C]-citrate is lost in the form of (14)CO(2). The isolation of a pseudomonad characterized by its ability to grow on tricarballylate as a sole carbon source has facilitated the study of the tricarboxylate-carrier specificity. Cells grown on citrate will exchange radioactive citrate for unlabelled citrate or isocitrate but not for cis-aconitate, trans-aconitate or tricarballylate. Cells grown on tricarballylate will exchange radioactive citrate for unlabelled citrate, cis-aconitate or tricarballylate, but not for isocitrate or trans-aconitate. The properties of the exchange system involved are compared with those of the related system in mitochondria.
D000498:Allyl Compounds / Q000378:metabolism; D002245:Carbon Dioxide / Q000096:biosynthesis; D002247:Carbon Isotopes; D002951:Citrates / Q000096:biosynthesis / Q000378:metabolism*; D005461:Fluorine; D007523:Isocitrates / Q000378:metabolism; D010084:Oxidation-Reduction; D011549:Pseudomonas / Q000254:growth & development / Q000378:metabolism*; D013045:Species Specificity; D014233:Tricarboxylic Acids / Q000378:metabolism*
D000498:Allyl Compounds; D002247:Carbon Isotopes; D002951:Citrates; D007523:Isocitrates; D014233:Tricarboxylic Acids; D002245:Carbon Dioxide; D005461:Fluorine