Incorporation of label from D- -hydroxy( 14 C)butyrate and (3- 14 C)acetoacetate into amino acids in rat brain in vivo.
The metabolism of ketone bodies by rat brain was studied in vivo. Rats starved for 48h were given either d-beta-hydroxy[3-(14)C]butyrate or [3-(14)C]acetoacetate by intravenous injection and killed after 3 or 10min. Total radioactivity in the acid-soluble material of the brain and the specific radioactivities of the brain amino acids glutamate, glutamine, aspartate and gamma-aminobutyrate were determined. A group of fed animals were also given d-beta-hydroxy[3-(14)C]butyrate. In the brains of all animals (14)C was present in the acid-soluble material and the specific radioactivity of glutamate was greater than that of glutamine.
D000090:Acetoacetates / Q000378:metabolism*; D000596:Amino Acids / Q000096:biosynthesis*; D000613:Aminobutyrates / Q000096:biosynthesis; D000818:Animals; D001224:Aspartic Acid / Q000096:biosynthesis; D001921:Brain / Q000378:metabolism*; D002247:Carbon Isotopes; D005971:Glutamates / Q000096:biosynthesis; D006885:Hydroxybutyrates / Q000378:metabolism*; D008297:Male; D051381:Rats; D013217:Starvation / Q000378:metabolism
D000090:Acetoacetates; D000596:Amino Acids; D000613:Aminobutyrates; D002247:Carbon Isotopes; D005971:Glutamates; D006885:Hydroxybutyrates; D001224:Aspartic Acid