Comparison of the metabolism of cholesterol, cholestanol, and  -sitosterol in L-cell mouse fibroblasts.
The following data have been obtained from comparative studies on the metabolism of cholesterol, cholestanol, and beta-sitosterol by L-cell mouse fibroblasts. (1) When the sterols are added to the growth medium under similar conditions, cellular incorporation of cholesterol > cholestanol > beta-sitosterol; (2) only limited cellular esterification of these compounds occurs; (3) no metabolic products arising from the sterols could be detected; (4) influx of all sterols is dependent upon the concentration; and (5) exogenous cholesterol reduces mevalonate incorporation into cellular sterol to a lesser extent than acetate or glucose. The metabolism of these sterols is discussed in relation to their ability to influence de novo sterol biosynthesis.
D000818:Animals; D002247:Carbon Isotopes; D002458:Cell Fractionation; D002776:Cholestanes / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism* / Q000494:pharmacology; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D046508:Culture Techniques; D004952:Esters; D005347:Fibroblasts / Q000378:metabolism*; D007739:L Cells / Q000378:metabolism*; D051379:Mice; D013261:Sterols / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism*; D013997:Time Factors; D014316:Tritium
D002247:Carbon Isotopes; D002776:Cholestanes; D004952:Esters; D013261:Sterols; D014316:Tritium; D002784:Cholesterol