Urea-inorganic phosphate mixtures as prebiotic phosphorylating agents.
Previous attempts to phosphorylate nucleosides by heating with inorganic phosphate succeeeded only when acid phosphates such as Ca(HPO(4))(2) were used. The addition of urea and ammonium chloride to the reaction mixture permits phosphorylation in high yield with neutral or basic phosphates at temperatures in the range of 65 degrees to 100 degrees C. Since the abundant mineral, hydroxylapatite, is a satisfactory substrate for this reaction, we believe that this procedure plausible model for prebiotic phosphorylation.
D000469:Alkaline Phosphatase; D000643:Ammonium Chloride; D000818:Animals; D001639:Bicarbonates; D002130:Calcium Phosphates; D002247:Carbon Isotopes; D055598:Chemical Phenomena; D002621:Chemistry; D002854:Chromatography, Paper; D006882:Hydroxyapatites; D009705:Nucleosides; D010710:Phosphates; D012260:Ribonucleases; D012911:Snakes; D013936:Thymidine; D014500:Uracil Nucleotides; D014508:Urea; D014529:Uridine; D014688:Venoms
D001639:Bicarbonates; D002130:Calcium Phosphates; D002247:Carbon Isotopes; D006882:Hydroxyapatites; D009705:Nucleosides; D010710:Phosphates; D014500:Uracil Nucleotides; D014688:Venoms; D000643:Ammonium Chloride; D014508:Urea; D012260:Ribonucleases; D000469:Alkaline Phosphatase; D013936:Thymidine; D014529:Uridine