Nucleic acid enzymology of extremely halophilic bacteria. Halobacterium cutirubrum ribonucleic acid-dependent ribonucleic acid polymerase.
1. Crude extracts of the extreme halophile Halobacterium cutirubrum contain separable DNA-dependent and RNA-dependent RNA polymerases. 2. The RNA-dependent enzyme has been purified about 2800-fold. 3. It requires RNA, preferably of high molecular weight, and all four ribonucleoside triphosphates to incorporate (14)C-labelled nucleoside triphosphate into an acid-insoluble, ribonuclease-sensitive product. 4. Both the stability and activity of the RNA polymerase are relatively insensitive to changes in potassium chloride or sodium chloride concentration, but incorporation is stimulated by both Mg(2+) and Mn(2+). 5. The molecular weight of the enzyme is about 17000-18000.
D000255:Adenosine Triphosphate / Q000378:metabolism; D002247:Carbon Isotopes; D002498:Centrifugation; D006217:Halobacterium / Q000201:enzymology*; D008274:Magnesium; D008345:Manganese; D008970:Molecular Weight; D010710:Phosphates; D011189:Potassium Chloride; D011506:Proteins / Q000032:analysis; D012313:RNA; D012316:RNA Nucleotidyltransferases / Q000302:isolation & purification*; D012965:Sodium Chloride; D013053:Spectrophotometry; D013698:Templates, Genetic
D002247:Carbon Isotopes; D010710:Phosphates; D011506:Proteins; D008345:Manganese; D012965:Sodium Chloride; D012313:RNA; D011189:Potassium Chloride; D000255:Adenosine Triphosphate; D012316:RNA Nucleotidyltransferases; D008274:Magnesium