A method for the determination of lipoprotein lipase in postheparin plasma and body tissues utilizing a triolein-coated Celite substrate.
A simple and specific method for assaying lipoprotein lipase activity is described. Postheparin plasma, heart homogenates, or extracts of acetone powder of adipose tissue were incubated with a triolein-coated Celite substrate, and enzyme activity was determined from the rate of free fatty acid (FFA) release in the incubation system. FFA release was linear for 30 min, and was proportional to protein concentration in the incubation system. FFA release was decreased by addition of deoxycholate or Triton X-100. Increasing the concentration of heparin in the incubation system caused a gradual decrease in FFA release by postheparin plasma and increases in activity of heart homogenates and adipose tissue lipoprotein lipase. The Celite substrate was found to be satisfactory for assaying pancreatic lipase activity as well.
D000273:Adipose Tissue / Q000187:drug effects / Q000201:enzymology*; D000818:Animals; D001647:Bile Acids and Salts / Q000494:pharmacology; D002417:Cattle; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D006321:Heart / Q000187:drug effects; D006493:Heparin / Q000494:pharmacology; D008071:Lipoprotein Lipase / Q000032:analysis / Q000097:blood*; D008722:Methods; D009206:Myocardium / Q000201:enzymology*; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013501:Surface-Active Agents; D014304:Triolein
D001647:Bile Acids and Salts; D005230:Fatty Acids, Nonesterified; D013501:Surface-Active Agents; D014304:Triolein; D012710:Serum Albumin, Bovine; D006493:Heparin; D008071:Lipoprotein Lipase