A rapid method for the purification of bovine thrombin and the inhibition of the purified enzyme wtih phenylmethylsulfonyl fluoride.
D000578:Amidines; D000818:Animals; D001120:Arginine; D002417:Cattle; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004355:Drug Stability; D004952:Esters; D005461:Fluorine; D006846:Hydrocarbons, Halogenated; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010710:Phosphates; D012964:Sodium; D013053:Spectrophotometry; D013451:Sulfonic Acids; D013917:Thrombin / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D013997:Time Factors; D014466:Ultraviolet Rays
D000578:Amidines; D004952:Esters; D006846:Hydrocarbons, Halogenated; D010710:Phosphates; D013451:Sulfonic Acids; D005461:Fluorine; D001120:Arginine; D012964:Sodium; D013917:Thrombin