The role of cholesteryl 14-methylhexadecanoate in peptide elongation reactions.
1. Peptide-elongation factors were purified from rat liver and human tonsils and the contents of cholesteryl 14-methylhexadecanoate were determined in fractions obtained during enzyme purification. The relative contents of this compound in purified enzyme preparations was several times higher than that in the crude starting material. Elongation factors from human tonsils contained a significantly larger quantity of the cholesteryl ester than enzyme from rat liver. 2. Transfer enzymes extracted with various organic solvents showed variable decreased activities in both binding and peptidization assay. The decrease of enzymic activity was proportional to the amount of cholesteryl 14-methylhexadecanoate extracted from a given enzymic preparation. In systems containing both extracted elongation factors the polyphenylalanine synthesis was limited by the residual activity of the less active transfer factor. 3. The original enzymic activity of extracted transferases was fully recovered by the addition of pure cholesteryl 14-methylhexadecanoate in quantities corresponding to those extracted. 4. Increase of the relative contents of this cholesteryl ester during enzyme purification, decrease of the enzymic activity after the extraction and its recovery by the addition of this compound indicates that the presence of this ester in elongation factors is essential for the normal function of these enzymes.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002848:Chromatography, DEAE-Cellulose; D006801:Humans; D008099:Liver / Q000378:metabolism; D014066:Palatine Tonsil / Q000378:metabolism; D010452:Peptide Biosynthesis; D010441:Peptide Chain Elongation, Translational; D012343:RNA, Transfer / Q000378:metabolism; D051381:Rats; D012997:Solvents; D013261:Sterols / Q000302:isolation & purification / Q000378:metabolism*; D014166:Transferases / Q000302:isolation & purification
D000596:Amino Acids; D012997:Solvents; D013261:Sterols; D012343:RNA, Transfer; D014166:Transferases