title: Isolation and identification of trehalase from Pullularia pullulans.
abstract: Trehalase has been isolated from Pullularia pullulans. The enzyme, which is specific for trehalose, was purified approximately 800-fold. The optimal pH was found to be 4.0 and the Michaelis dissociation constant, K(m), was determined to be 3.2 x 10(-3)m.
mesh_terms: D050260:Carbohydrate Metabolism; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D002848:Chromatography, DEAE-Cellulose; D003470:Culture Media; D004187:Disaccharides / Q000096:biosynthesis; D006026:Glycoside Hydrolases / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D003904:Mitosporic Fungi / Q000032:analysis / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D000644:Quaternary Ammonium Compounds; D013431:Sulfates; D014461:Ultracentrifugation
chemical_list: D003470:Culture Media; D004187:Disaccharides; D011506:Proteins; D000644:Quaternary Ammonium Compounds; D013431:Sulfates; D006026:Glycoside Hydrolases