title: Electron microscope histochemical evidence for a partial or total block of the tricarboxylic acid cycle in the mitochondria of presynaptic axon terminals.
abstract: Respiration-linked, massive accumulation of Sr(2+) is used to reveal the coupled oxidation of pyruvate, alpha-oxoglutarate, succinate, and malate by in situ mitochondria. All of these substrates were actively oxidized in the dendritic and perikaryal mitochondria, but no alpha-oxoglutarate or succinate utilization could be demonstrated in the mitochondria of the presynaptic axon terminals. A block at an early step of alpha-oxoglutarate and succinate oxidation is proposed to account for the negative histochemical results, since the positive reaction with pyruvate and malate proves that these mitochondria possess an intact respiratory chain and energy-coupling mechanism essential for Sr(2+) accumulation. This indicates that the mitochondria in the axon terminals would be able to generate energy for synaptic function with at least some of the respiratory substrates. With regard to the block in the tricarboxylic acid cycle, the oxaloacetate necessary for citrate formation is suggested to be provided by fixation of CO(2) into some of the pyruvate.
mesh_terms: D001369:Axons / Q000378:metabolism*; D002421:Caudate Nucleus / Q000166:cytology; D002525:Cerebellar Cortex / Q000166:cytology; D002540:Cerebral Cortex / Q000166:cytology; D002952:Citric Acid Cycle; D003712:Dendrites / Q000378:metabolism; D005977:Glutarates / Q000378:metabolism; D006651:Histocytochemistry; D008293:Malates / Q000378:metabolism; D008854:Microscopy, Electron; D008928:Mitochondria / Q000032:analysis / Q000378:metabolism*; D010084:Oxidation-Reduction; D011773:Pyruvates / Q000378:metabolism; D013116:Spinal Cord / Q000166:cytology; D013324:Strontium / Q000032:analysis / Q000378:metabolism; D013386:Succinates / Q000378:metabolism; D013788:Thalamus / Q000166:cytology
chemical_list: D005977:Glutarates; D008293:Malates; D011773:Pyruvates; D013386:Succinates; D013324:Strontium