Metabolic effects of the major component of bovine growth hormone.
Bovine growth hormone, subjected to DEAE-cellulose chromatography, yielded one major and several minor components. The various chromatographic fractions of bovine growth hormone were compared with the parent material for their ability to promote hormone effects in vivo and in vitro. The major component of bovine growth hormone was homogeneous by acrylamide-gel electrophoresis, rechromatography and sedimentation equilibrium. Its amino acid composition was similar to that of the parent hormone. The major component possessed all the qualitative activities present in the original heterogeneous material, including promotion of acute hypoglycaemia and hypolipaemia. In studies in vitro in adipose-tissue segments the major component of the hormone increased entry of glucose and its oxidation to CO(2), conversion of glucose into glyceride glycerol, release of glycerol and incorporation of histidine into adiposetissue protein. Other chromatographic fractions of bovine growth hormone were not homogeneous and possessed some but not all of the metabolic activities attributed to the hormone preparations or its major component. Thus, the metabolic effects obtained with bovine growth-hormone preparations in vivo and in vitro can be obtained with the major homogeneous component of the hormone. This observation precludes the possibility that the metabolic effects obtained with bovine growth-hormone preparations are due to the combined actions of a number of components found therein.
D000179:Acrylates; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001681:Biological Assay; D002245:Carbon Dioxide / Q000378:metabolism; D002417:Cattle; D002848:Chromatography, DEAE-Cellulose; D004589:Electrophoresis, Disc; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005782:Gels; D005947:Glucose / Q000378:metabolism; D005989:Glycerides / Q000378:metabolism; D013006:Growth Hormone / Q000032:analysis / Q000494:pharmacology*; D006639:Histidine / Q000378:metabolism; D006949:Hyperlipidemias; D007003:Hypoglycemia; D066298:In Vitro Techniques; D008660:Metabolism / Q000187:drug effects*; D008970:Molecular Weight; D051381:Rats; D014461:Ultracentrifugation
D000179:Acrylates; D000596:Amino Acids; D005230:Fatty Acids, Nonesterified; D005782:Gels; D005989:Glycerides; D002245:Carbon Dioxide; D006639:Histidine; D013006:Growth Hormone; D005947:Glucose