Detection of arginine dihydrolase in nonfermentative gram-negative bacteria by use of thin-layer chromatography.
Thin-layer chromatography was used to detect the presence of the arginine dihydrolase system in nonfermentative gram-negative bacteria. The test was positive for the fluorescent pseudomonads as well as Pseudomonas maltophilia, whereas other Pseudomonas sp., Mima, and Herellea were negative. This procedure can be completed in a few hours and may be useful in the clinical laboratory.
D000150:Acinetobacter / Q000201:enzymology / Q000378:metabolism; D001120:Arginine / Q000378:metabolism; D001419:Bacteria / Q000201:enzymology*; D002855:Chromatography, Thin Layer; D002956:Citrulline / Q000096:biosynthesis; D006146:Guanidines / Q000096:biosynthesis; D006867:Hydrolases / Q000032:analysis* / Q000096:biosynthesis / Q000378:metabolism; D008722:Methods; D009952:Ornithine / Q000096:biosynthesis; D011549:Pseudomonas / Q000201:enzymology* / Q000378:metabolism; D013045:Species Specificity
D006146:Guanidines; D002956:Citrulline; D001120:Arginine; D009952:Ornithine; D006867:Hydrolases