title: Cell-mediated hypersensitivity in rabbits infected with Trypanosoma brucei and Trypanosoma rhodesiense.
abstract: Animals infected with strains of Trypanosoma brucei and T. rhodesiense exhibited cutaneous hypersensitivity to intradermal administration of antigen. This reactivity was of two types, an Arthus-type, antibody-mediated reaction which reached maximum intensity 4 hr after injection and a delayed-type, cell-mediated reaction which reached maximum intensity 24 hr after injection. This delayed-type hypersensitivity appeared in rabbits not earlier than 3 weeks after onset of infection. It did not occur in animals which received dead organisms. There was a cross-reaction in both types of reactivity between antigens prepared from T. brucei and T. rhodesiense. The delayed-type hypersensitivity was transferred to normal rabbits by intravenous inoculation of washed living cells from the spleen of a rabbit which showed delayed hypersensitivity. Dead cells failed to transfer hypersensitivity. The histological picture of the indurated area was typical of a delayed-type reaction.
mesh_terms: D000818:Animals; D000941:Antigens / Q000008:administration & dosage / Q000302:isolation & purification; D003429:Cross Reactions; D006968:Hypersensitivity, Delayed / Q000209:etiology* / Q000473:pathology; D007271:Injections, Intradermal; D011817:Rabbits / Q000276:immunology; D012867:Skin / Q000473:pathology; D012882:Skin Tests; D013997:Time Factors; D014345:Trypanosoma / Q000276:immunology*; D014346:Trypanosoma brucei brucei / Q000276:immunology*; D014352:Trypanosomiasis / Q000150:complications*
chemical_list: D000941:Antigens