Comparative zone electrophoresis of enzymes of Pseudomonas solanacearum and Pseudomonas cepacia.
The technique of starch-gel electrophoresis with specific staining for a series of enzymes was used to compare 21 Pseudomonas strains representing both P. cepacia and P. solanacearum. These experiments produced no evidence for close similarity of the two species. Twelve strains of P. solanacearum were compared by means of data obtained from nine different enzymes, and the data indicate that these strains belong in two biotypes. Except for the assignment of two strains, these groups are the same as the two major groups previously derived from nutritional properties and from deoxyribonucleic acid hybridization experiments. Eleven enzymes were available for comparisons of the P. cepacia strains. Eight of these strains form a homogeneous group, but the last strain, number 249, differs considerably from the other representatives of the species.
D000429:Alcohol Oxidoreductases / Q000032:analysis*; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D003470:Culture Media; D003596:Cytosine / Q000032:analysis; D004269:DNA, Bacterial / Q000032:analysis; D004586:Electrophoresis; D004950:Esterases / Q000032:analysis*; D005650:Fumarates; D005782:Gels; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D005969:Glutamate Dehydrogenase / Q000032:analysis; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000032:analysis; D006147:Guanine / Q000032:analysis; D006836:Hydro-Lyases / Q000032:analysis*; D007521:Isocitrate Dehydrogenase / Q000032:analysis; D008291:Malate Dehydrogenase / Q000032:analysis; D010088:Oxidoreductases / Q000032:analysis*; D010734:Phosphogluconate Dehydrogenase / Q000032:analysis; D011549:Pseudomonas / Q000032:analysis / Q000145:classification* / Q000201:enzymology / Q000254:growth & development; D011809:Quinones; D013213:Starch; D013778:Tetrazolium Salts; D014969:Xanthine Oxidase / Q000032:analysis
D002712:Chlorides; D003470:Culture Media; D004269:DNA, Bacterial; D005650:Fumarates; D005782:Gels; D011809:Quinones; D013778:Tetrazolium Salts; D002586:Cesium; D006147:Guanine; D003596:Cytosine; D013213:Starch; D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D014969:Xanthine Oxidase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D005969:Glutamate Dehydrogenase; D004950:Esterases; D006836:Hydro-Lyases