Studies on flavonoid metabolism. Biliary and urinary excretion of metabolites of (+)-(U- 14 C)catechin.
1. The biliary and urinary excretion of (+)-[U-(14)C]catechin was studied in normal male rats after a single injection of the flavonoid. 2. In rats large amounts of radioactivity (33.6-44.3% of the dose in 24h) were excreted in the bile as two glucuronide conjugates [one of which was a (+)-catechin conjugate] and three other unconjugated metabolites. 3. Excretion of radioactivity in the urine when the bile duct was not cannulated amounted to 44.5% of the dose. 4. In both the urine and bile the new metabolites showed maximum excretion in the (1/2)-1(1/2)h after intravenous injection of [(14)C]catechin. 5. The metabolites m-hydroxyphenylpropionic acid, p-hydroxyphenylpropionic acid, delta-(3-hydroxyphenyl)-gamma-valerolactone and delta-(3,4-dihydroxyphenyl)-gamma-valerolactione originate from the action of the intestinal micro-organisms on the biliary-excreted metabolites of (+)-catechin. These phenolic acid and lactone metabolites are then reabsorped and excreted in the urine. 6. It is proposed that, depending on the route of administration of (+)-catechin, there exists an alternative pathway, involving biliary excretion, for the metabolism of (+)-catechin.
D000818:Animals; D001646:Bile / Q000378:metabolism*; D002247:Carbon Isotopes; D002392:Catechin / Q000378:metabolism / Q000652:urine; D005419:Flavonoids / Q000378:metabolism* / Q000652:urine; D005965:Glucuronates / Q000378:metabolism; D007275:Injections, Intravenous; D007408:Intestinal Absorption; D007422:Intestines / Q000382:microbiology; D007783:Lactones / Q000096:biosynthesis / Q000378:metabolism; D008297:Male; D010636:Phenols / Q000096:biosynthesis / Q000378:metabolism; D011422:Propionates / Q000096:biosynthesis / Q000378:metabolism; D051381:Rats
D002247:Carbon Isotopes; D005419:Flavonoids; D005965:Glucuronates; D007783:Lactones; D010636:Phenols; D011422:Propionates; D002392:Catechin