Enzymic iodination. A probe for accessible surface proteins of normal and neoplastic lymphocytes.
1. Radioactive iodide was covalently bound to living cells from normal mouse spleen and a variety of lymphoid tumours by a system consisting of lactoperoxidase, hydrogen peroxide and iodide. 2. About 3x10(5)-6x10(5) molecules of [(125)I]iodide/cell could be incorporated without affecting cell viability. 3. Electron-micrographic radioautography showed that the radioactive label was associated with the outer surfaces of the cells. 4. Radioiodinated proteins were solubilized in 9m-urea-0.2m-mercaptoethanol and analysed by gel-filtration and disc electrophoresis. 5. Comparison of distinct tumour lines by disc electrophoresis showed qualitative and quantitative differences in protein distribution patterns.
D000818:Animals; D001345:Autoradiography; D002460:Cell Line; D002462:Cell Membrane / Q000032:analysis*; D002850:Chromatography, Gel; D004589:Electrophoresis, Disc; D006861:Hydrogen Peroxide; D007454:Iodides; D007456:Iodine Isotopes; D008214:Lymphocytes / Q000032:analysis*; D008223:Lymphoma / Q000378:metabolism*; D008623:Mercaptoethanol; D051379:Mice; D008854:Microscopy, Electron; D009101:Multiple Myeloma / Q000378:metabolism; D009363:Neoplasm Proteins / Q000032:analysis*; D010544:Peroxidases; D011506:Proteins / Q000032:analysis*; D013154:Spleen / Q000166:cytology; D013945:Thymoma / Q000378:metabolism; D014508:Urea
D007454:Iodides; D007456:Iodine Isotopes; D009363:Neoplasm Proteins; D011506:Proteins; D008623:Mercaptoethanol; D014508:Urea; D006861:Hydrogen Peroxide; D010544:Peroxidases