The absorption, distribution and excretion of  3 H-chlorambucil in rats bearing the Yoshida ascites sarcoma.
The distribution of (3)H-chlorambucil following its administration by subcutaneous injection to Yoshida ascites tumour-bearing rats has been examined, in an attempt to elucidate the metabolic fate and mode of action of this drug.Drug uptake into the body tissue was rapid, with a high level of radioactivity being associated with the plasma and ascitic fluid during the initial 6-hour period after treatment. Previous studies in vitro had shown that chlorambucil-resistant cells accumulated less drug than their sensitive counterparts: this discrepancy was also observed after in vivo drug treatment and was reflected in the two-fold difference in extent of binding of tritium to DNA, RNA and protein isolated from the 2 cell strains. These results might in part explain the observed difference in metabolism of chlorambucil by the resistant and sensitive strain of Yoshida ascites sarcoma cells.
D000818:Animals; D001202:Ascitic Fluid / Q000378:metabolism; D002699:Chlorambucil / Q000097:blood / Q000378:metabolism* / Q000652:urine; D004273:DNA, Neoplasm / Q000378:metabolism; D007279:Injections, Subcutaneous; D009363:Neoplasm Proteins / Q000378:metabolism; D012334:RNA, Neoplasm / Q000378:metabolism; D051381:Rats; D012517:Sarcoma, Yoshida / Q000378:metabolism* / Q000652:urine; D014316:Tritium
D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; D014316:Tritium; D002699:Chlorambucil