title: Oxidation of nicotinic acid by a Bacillus species: purification and properties of nicotinic acid and 6-hydroxynicotinic acid hydroxylases.
abstract: The enzymes of a Bacillus species that hydroxylate nicotinic acid to 6-hydroxynicotinic acid and 6-hydroxynicotinic acid to 2,6-dihydroxynicotinic acid were purified and characterized. The purified enzymes contained approximately two molecules of flavine and eight molecules of iron per molecule of enzyme. The enzymes were large (molecular weight, 400,000 to 450,000) and appeared to consist of subunits.
mesh_terms: D000645:Ammonium Sulfate; D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D002474:Cell-Free System; D011232:Chemical Precipitation; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003470:Culture Media; D004586:Electrophoresis; D005415:Flavins / Q000032:analysis; D005782:Gels; D006900:Hydroxylation; D007501:Iron / Q000032:analysis; D006899:Mixed Function Oxygenases / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D008970:Molecular Weight; D009539:Nicotinic Acids / Q000378:metabolism*; D013053:Spectrophotometry; D014461:Ultracentrifugation
chemical_list: D003470:Culture Media; D005415:Flavins; D005782:Gels; D009539:Nicotinic Acids; D007501:Iron; D006899:Mixed Function Oxygenases; D000645:Ammonium Sulfate