title: Viable, avirulent Leptospira interrogans serotype pomona vaccine: preservation in liquid nitrogen.
abstract: By the addition of glycerol (10%, v/v), viability titers of serotype pomona vaccine were maintained within 1 to 2 logs (base 10) during controlled-rate freezing and storage in liquid nitrogen.
mesh_terms: D000418:Albumins; D001205:Ascorbic Acid; D001428:Bacterial Vaccines / Q000592:standards*; D004121:Dimethyl Sulfoxide; D004356:Drug Storage; D005615:Freezing; D005990:Glycerol; D007919:Leptospira / Q000472:pathogenicity; D009584:Nitrogen; D011134:Polysaccharides; D012703:Serotyping; D014774:Virulence
chemical_list: D000418:Albumins; D001428:Bacterial Vaccines; D011134:Polysaccharides; D009584:Nitrogen; D005990:Glycerol; D001205:Ascorbic Acid; D004121:Dimethyl Sulfoxide