Rabbit Tamm-Horsfall urinary glycoprotein. Chemical composition and subunit structure.
1. Tamm-Horsfall glycoprotein from rabbit urine has been isolated and characterized. The homogeneity of the preparation has been established by a variety of procedures including disc gel electrophoresis and ultracentrifugation in aqueous solution, sodium dodecyl sulphate and formic acid. 2. The chemical composition has been determined and a carbohydrate content of approx. 31% was obtained. The relative contents of the amino acids were shown to be very similar to those in human Tamm-Horsfall glycoprotein. A trace of lipid was also detected. 3. Leucine was identified as the only N-terminal amino acid. 4. The subunit structure was investigated in the presence of sodium dodecyl sulphate by gel filtration and disc gel electrophoresis. These studies indicated that the subunit possessed a molecular weight of approx. 84000+/-6000. A similar value was obtained after reduction and S-alkylation of the glycoprotein indicating that the disulphide bonds were all intrachain. 5. A minimum value for the chemical molecular weight of 85000+/-6000 was obtained from the number of N-terminal amino acids released by cyanogen bromide cleavage of the glycoprotein. 6. The immunological properties of the glycoprotein were studied. Cross reactivity was demonstrated between human Tamm-Horsfall glycoprotein and a guinea-pig anti-rabbit Tamm-Horsfall antiserum.
D000478:Alkylation; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002850:Chromatography, Gel; D003429:Cross Reactions; D004589:Electrophoresis, Disc; D005782:Gels; D006023:Glycoproteins / Q000032:analysis / Q000652:urine*; D006168:Guinea Pigs; D006801:Humans; D007106:Immune Sera; D008055:Lipids / Q000032:analysis; D008970:Molecular Weight; D010084:Oxidation-Reduction; D011817:Rabbits; D013501:Surface-Active Agents; D014461:Ultracentrifugation
D000596:Amino Acids; D002241:Carbohydrates; D005782:Gels; D006023:Glycoproteins; D007106:Immune Sera; D008055:Lipids; D013501:Surface-Active Agents