Utilization of palmitic acid by Mycobacterium avium.
Mycobacterium avium accumulates (14)C-palmitic acid with saturation kinetics; the process is both temperature dependent and pH sensitive. The fatty acid is incorporated into triglyceride in vivo and the conversion is detectable within 5 min after exposure of the cells to (14)C-palmitic acid. The triglyceride is rapidly utilized because (14)CO(2) evolution from it begins within 30 min after (14)C-palmitic acid accumulation. Data from silicic acid column chromatography of extracts of cultures that have divided many times in medium containing (14)C-palmitic acid indicate that a large proportion of the cell lipid is triglyceride, but the radioactivity is widely dispersed among the other lipids. It is estimated that about 5% of the cell dry weight is triglyceride in a postexponential culture.
D002241:Carbohydrates / Q000032:analysis; D002245:Carbon Dioxide / Q000378:metabolism; D002247:Carbon Isotopes; D002845:Chromatography; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008055:Lipids / Q000032:analysis; D009161:Mycobacterium / Q000032:analysis / Q000378:metabolism*; D010169:Palmitic Acids / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D014280:Triglycerides / Q000032:analysis / Q000096:biosynthesis
D002241:Carbohydrates; D002247:Carbon Isotopes; D003470:Culture Media; D008055:Lipids; D010169:Palmitic Acids; D014280:Triglycerides; D002245:Carbon Dioxide