Metabolism of pyrimidine nucleotides in a microorganism. 3. Enzymatic production of ribose-5-phosphate from uridine-5'-monophosphate by Pseudomonas oleovorans.
A study was made to develop a new method for the production of ribose-5-phosphate (R-5-P) from uridine-5'-monophosphate (UMP) by the action of nucleotide-N-ribosidase of Pseudomonas oleovorans, and a suitable medium for the formation of nucleotide-N-ribosidase was established. For the enzymatic conversion of UMP to R-5-P, a cell suspension was employed as the enzyme source. Although degradation of R-5-P, the desired product, occurred during the course of the enzyme reaction, it was prevented by the addition of an appropriate amount of zinc ion and resulted in a stoichiometric conversion of UMP to R-5-P and uracil. Accumulated R-5-P was readily isolated by ion-exchange chromatography of the bacteria-free reaction mixture. Yield of isolated R-5-P was about 60% of the theoretical recovery.
D000042:Absorption; D000643:Ammonium Chloride / Q000494:pharmacology; D001464:Barium; D002474:Cell-Free System; D011232:Chemical Precipitation; D002854:Chromatography, Paper; D003470:Culture Media; D005374:Filtration; D005650:Fumarates / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007259:Infrared Rays; D010428:Pentosephosphates / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D010430:Pentosyltransferases / Q000096:biosynthesis / Q000378:metabolism*; D010461:Peptones / Q000494:pharmacology; D011549:Pseudomonas / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D012266:Ribose; D013057:Spectrum Analysis; D013696:Temperature; D013997:Time Factors; D014498:Uracil / Q000096:biosynthesis; D014500:Uracil Nucleotides / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology
D003470:Culture Media; D005650:Fumarates; D010428:Pentosephosphates; D010461:Peptones; D014500:Uracil Nucleotides; D000643:Ammonium Chloride; D001464:Barium; D014498:Uracil; D012266:Ribose; D010430:Pentosyltransferases; D015032:Zinc