title: Consequences of the inhibition of cardiolipin metabolism in Haemophilus parainfluenzae.
abstract: Examination of phospholipid metabolism in Haemophilus parainfluenzae with inhibitors of various cellular functions indicated that macromolecular synthesis and lipid metabolism can be dissociated at least for a short time. Two classes of inhibitors have relatively specific effects on cardiolipin (CL) metabolism. Pentachlorophenol and p-hydroxymercuribenzoate blocked CL synthesis but allowed CL hydrolysis to phosphatidic acid and phosphatidyl glycerol (PG); 3,3',4,5'-tetrachlorosalicylanilide (TCS) and carbonyl cyanide m-chlorophenylhydrazone (m-CCCP) blocked CL hydrolysis with the stoichiometric accumulation of CL. It appeared as if TCS and m-CCCP inhibited a vital activity coupled with the hydrolysis of CL by the highly active, CL-specific phospholipase D found in this organism. Because TCS and m-CCCP are thought to act by destroying the proton gradient thereby interrupting energy-dependent transport, it is possible that a highly active portion of the cellular CL could be coupled to some phase of this process.
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000963:Antimetabolites / Q000494:pharmacology; D001345:Autoradiography; D002247:Carbon Isotopes; D002854:Chromatography, Paper; D003124:Colorimetry; D003545:Cysteine / Q000494:pharmacology; D006190:Haemophilus / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D006868:Hydrolysis; D010710:Phosphates / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D010759:Phosphorus Isotopes; D013501:Surface-Active Agents
chemical_list: D000900:Anti-Bacterial Agents; D000963:Antimetabolites; D002247:Carbon Isotopes; D010710:Phosphates; D010743:Phospholipids; D010759:Phosphorus Isotopes; D013501:Surface-Active Agents; D010740:Phospholipases; D003545:Cysteine