Improved method for virus structural polypeptide analysis on dissociating acylamide gel.
A method is described in which polypeptides can be separated, with a high band resolution, by electrophoresis through "pore gradient" acrylamide gel (15 cm in length) in a sodium dodecyl sulfate-urea dissociating system. The applicability of this technique to the analysis of virus structural components was examined with the adenovirus type 2 model system.
D000256:Adenoviridae / Q000032:analysis* / Q000302:isolation & purification; D002277:Carcinoma; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D004589:Electrophoresis, Disc / Q000592:standards; D006801:Humans; D008722:Methods; D008970:Molecular Weight; D009062:Mouth Neoplasms; D010455:Peptides / Q000032:analysis*; D012964:Sodium; D013431:Sulfates; D014508:Urea; D014776:Virus Cultivation
D002712:Chlorides; D010455:Peptides; D013431:Sulfates; D002586:Cesium; D014508:Urea; D012964:Sodium