Oxidation of nicotinic acid by a Bacillus species: source of oxygen atoms for the hydroxylation of nicotinic acid and 6-hydroxynicotinic acid.
Three types of evidence are presented to show that the enzymes that hydroxylate nicotinic acid to 2,6-dihydroxynicotinic acid use water as a source of oxygen atoms. (18)O is incorporated into the products from H(2) (18)O. Molecular oxygen acts as a terminal electron acceptor, one-half molecule being consumed per molecule of hydroxyl groups incorporated. An external electron acceptor is required for activity in purified preparations.
D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D002474:Cell-Free System; D002625:Chemistry, Organic; D002848:Chromatography, DEAE-Cellulose; D003470:Culture Media; D004579:Electron Transport; D006900:Hydroxylation; D013058:Mass Spectrometry; D006899:Mixed Function Oxygenases / Q000302:isolation & purification / Q000378:metabolism*; D009539:Nicotinic Acids / Q000378:metabolism*; D055601:Organic Chemistry Phenomena; D010100:Oxygen; D010101:Oxygen Consumption; D010103:Oxygen Isotopes; D014867:Water / Q000378:metabolism*
D003470:Culture Media; D009539:Nicotinic Acids; D010103:Oxygen Isotopes; D014867:Water; D006899:Mixed Function Oxygenases; D010100:Oxygen