Ferrochelatase and  -aminolaevulate synthetase in brain, heart, kidney and liver of normal and porphyric rats. The induction of  -aminolaevulate synthetase in kidney cytosol and mitochondria by allylisopropylacetamide.
1. delta-Aminolaevulate synthetase was detected in liver and kidney mitochondria prepared from normal rats. 2. The administration of allylisopropylacetamide induced an increase in delta-aminolaevulate synthetase in both liver and kidney mitochondria and the enzyme also appeared in the cytosol fraction of both tissues. Comparison with the distribution of glutamate dehydrogenase indicated that this soluble kidney delta-aminolaevulate synthetase was truly of cytosol origin and did not arise from disrupted mitochondria. The kidney cytosol enzyme was inhibited by 50% by 50mum-protohaem. 3. delta-Aminolaevulate synthetase could not be detected in mitochondria or cytosol from heart or brain from normal or porphyric rats. 4. The administration of allylisopropylacetamide caused little or no increase in ferrochelatase or cytochrome content of liver, kidney, heart or brain mitochondria.
D000081:Acetamides / Q000494:pharmacology; D000498:Allyl Compounds / Q000494:pharmacology; D000577:Amides / Q000494:pharmacology*; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000201:enzymology; D004790:Enzyme Induction; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D007668:Kidney / Q000201:enzymology; D007982:Levulinic Acids / Q000378:metabolism; D008025:Ligases / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D008190:Lyases / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D011164:Porphyrias / Q000139:chemically induced / Q000201:enzymology*; D011166:Porphyrins / Q000096:biosynthesis; D051381:Rats
D000081:Acetamides; D000498:Allyl Compounds; D000577:Amides; D000596:Amino Acids; D007982:Levulinic Acids; D011166:Porphyrins; D007501:Iron; D005969:Glutamate Dehydrogenase; D008190:Lyases; D008025:Ligases