title: The long-term storage of blood for transfusion using an improved container for freezing the red cells in liquid nitrogen.
abstract: Considerable experience has been gained in the operation of a bank of blood frozen in liquid nitrogen. The procedure for freezing and recovering the red cells is, in principle, that described by Krijnen, Kuivenhoven, and de Wit (1970). An improved metal freezing container offers greater freedom from liquid nitrogen leaks and hence, bacterial contamination. Over 500 units of blood have been preserved and used for transfusions without mishap, and many advantages are seen in this relatively economical method for the long-term storage of blood.
mesh_terms: D000535:Aluminum; D001792:Blood Platelets; D001793:Blood Preservation; D001798:Blood Proteins / Q000032:analysis; D004912:Erythrocytes; D005615:Freezing; D005990:Glycerol; D006452:Hemoglobinometry; D006801:Humans; D007958:Leukocyte Count; D008722:Methods; D009584:Nitrogen; D009996:Osmotic Fragility; D011188:Potassium / Q000097:blood; D012964:Sodium / Q000097:blood; D013057:Spectrum Analysis; D014867:Water
chemical_list: D001798:Blood Proteins; D014867:Water; D012964:Sodium; D000535:Aluminum; D009584:Nitrogen; D005990:Glycerol; D011188:Potassium