Microbial esterase detection with ultraviolet fluorescence.
A method is presented to identify esterase-synthesizing microbial colonies within mixed culture plates by utilizing induced esterase hydrolysis of nonfluorescent butyryl ester of 7-hydroxy-4-methylcoumarin to the highly fluorescent 7-hydroxy-4 methyl umbelliferone. Microscopy procedures for making esterase loci of fungal mycelia visible with this reaction are described.
D001230:Aspergillus / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism; D003374:Coumarins / Q000378:metabolism; D004950:Esterases / Q000096:biosynthesis / Q000302:isolation & purification* / Q000378:metabolism; D005658:Fungi / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism; D006868:Hydrolysis; D008722:Methods; D008856:Microscopy, Fluorescence; D012988:Soil Microbiology; D014466:Ultraviolet Rays
D003374:Coumarins; D004950:Esterases