Cell-free passive transfer of delayed hypersensitivity to chemicals in guinea pigs.
Delayed-type cutaneous reactivity to 2,4-dinitrochlorobenzene and 2,4-dinitrofluorobenzene in guinea pigs was transferred passively by fluids in which leukocytes from sensitive animals were incubated. Cells from peritoneal exudates, lymph nodes, and alveolar washings were employed. The cell-free transfer material was dialyzable, of small molecular size, and stable to 56 C for 30 min and -65 C for at least 9 weeks. It gave a ratio at 280 to 260 nm of 0.71. The relationships between temperature, pH, and cellular release of the transfer material were studied.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D002474:Cell-Free System; D002477:Cells; D002713:Chlorine / Q000009:adverse effects; D003429:Cross Reactions; D005122:Exudates and Transudates; D005260:Female; D005461:Fluorine / Q000009:adverse effects; D006168:Guinea Pigs; D006863:Hydrogen-Ion Concentration; D006968:Hypersensitivity, Delayed / Q000139:chemically induced*; D007116:Immunization, Passive; D007958:Leukocyte Count; D007962:Leukocytes / Q000187:drug effects; D008198:Lymph Nodes / Q000276:immunology; D008297:Male; D009578:Nitrobenzenes / Q000009:adverse effects*; D010537:Peritoneum / Q000276:immunology; D011233:Precipitin Tests; D012882:Skin Tests; D013057:Spectrum Analysis; D013696:Temperature
D000906:Antibodies; D009578:Nitrobenzenes; D005461:Fluorine; D002713:Chlorine