Isolation and partial characterization of methylated arginines from the encephalitogenic basic protein of myelin.
Two methylated derivatives of arginine were isolated from the encephalitogenic protein of myelin from the central nervous system. Evidence is presented for the proposed structures, omega-NN'-dimethylarginine and omega-N-monomethylarginine. In the encephalitogenic protein from human brain the proportion 1:6:10 for arginine:monomethylarginine:dimethylarginine residues was found to occur at position 107. Possible roles for the methylated arginine in conformational changes and altered ion-exchange behaviour are discussed.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001120:Arginine / Q000302:isolation & purification*; D001923:Brain Chemistry; D002417:Cattle; D002845:Chromatography; D004586:Electrophoresis; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000209:etiology*; D006801:Humans; D006868:Hydrolysis; D008745:Methylation; D009186:Myelin Sheath / Q000032:analysis*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D010455:Peptides / Q000032:analysis; D013116:Spinal Cord / Q000032:analysis
D000596:Amino Acids; D009419:Nerve Tissue Proteins; D010455:Peptides; D001120:Arginine