Studies on the binding and distribution of radioactively labelled 3'-methylcholanthrene in subcellular fractions of rat liver.
The subcellular distribution of either [(14)C] or [(3)H]3'-methylcholanthrene was studied in rat liver following a single intraperitoneal injection of the labelled hydrocarbon 10 hours previously.Adsorbed or non-covalently bound methylcholanthrene and its metabolic derivatives occurred in all cell fractions studied with the exception of purified cell walls. The highest specific activities (d.p.m./mg. protein) were found in washed mitochondria, microsomes and ribosome-free microsomal membranes.Covalent binding of methylcholanthrene and its metabolic derivatives to different cell fractions of rat liver occurs to a small extent and is considered not to be significant. The highest degree of binding occurs in washed mitochondria, microsomes, ribosome-free microsomal membranes and their constituent core proteins.Cell sap which contains non-covalently bound 3'-methylcholanthrene was fractionated into pH 5 enzyme and pH 5 supernatant fractions. The pH 5 enzyme fraction which possesses a high specific activity (d.p.m./mg. protein) was further fractionated with ammonium sulphate into three fractions. The 0-30% ammonium sulphate fraction had the highest specific activity.
D000327:Adsorption; D000645:Ammonium Sulfate; D000818:Animals; D002247:Carbon Isotopes; D002458:Cell Fractionation; D002462:Cell Membrane / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007274:Injections, Intraperitoneal; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D008566:Membranes / Q000378:metabolism; D008748:Methylcholanthrene / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism; D011485:Protein Binding; D012313:RNA / Q000378:metabolism; D051381:Rats; D012270:Ribosomes / Q000378:metabolism; D014316:Tritium
D002247:Carbon Isotopes; D014316:Tritium; D008748:Methylcholanthrene; D012313:RNA; D000645:Ammonium Sulfate