Structural proteins of adenovirus-associated viruses.
The structural proteins of adenovirus-associated virus (AAV) types 1, 2, and 3 were analyzed by acrylamide gel electrophoresis. In each case, one major protein (C) and two minor proteins (A and B) were identified. Component C had an estimated molecular weight of 62,000 daltons, and the molecular weights of components A and B were found to be 87,000 and 73,000 daltons, respectively. Coelectrophoresis of adenovirus and AAV proteins revealed an overlap only between the adenovirus fiber-penton component and the AAV C polypeptide. Among AAV serotypes, homologous components were electrophoretically identical, except that the C component of AAV-2 was of slightly lower molecular weight than the C components of AAV-1 and AAV-3. The relative incorporation of (14)C-arginine and (14)C-mixed amino acids into the three polypeptides of AAV-2 was similar, indicating an absence of an arginine-rich component. In addition, AAV-2 was found to have a substantially lower arginine content than helper adenoviruses.
D000256:Adenoviridae / Q000032:analysis*; D000596:Amino Acids / Q000032:analysis; D001120:Arginine / Q000032:analysis; D001322:Autoanalysis; D002247:Carbon Isotopes; D002277:Carcinoma; D002460:Cell Line; D004589:Electrophoresis, Disc; D006801:Humans; D008970:Molecular Weight; D009062:Mouth Neoplasms; D010455:Peptides / Q000032:analysis; D012526:Satellite Viruses / Q000032:analysis; D012703:Serotyping; D013045:Species Specificity; D014316:Tritium; D014764:Viral Proteins / Q000032:analysis*; D014776:Virus Cultivation
D000596:Amino Acids; D002247:Carbon Isotopes; D010455:Peptides; D014764:Viral Proteins; D014316:Tritium; D001120:Arginine