The uptake and efflux of glycine from rat cerebral-cortex slices.
The kinetics of the influx and efflux of radioactive l-glycine was studied in slices of rat cerebral cortex. The influx showed saturation kinetics and was inhibited by l-alanine. Influx was dependent on the presence of Na(+) ions and a metabolizable substrate. The efflux of glycine was accelerated by alanine. It was concluded that carrier-mediated facilitated diffusion was the mechanism of glycine uptake by, and efflux from, cerebral slices.
D000255:Adenosine Triphosphate / Q000032:analysis; D000409:Alanine / Q000494:pharmacology; D000818:Animals; D002247:Carbon Isotopes; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D005947:Glucose; D005977:Glutarates; D005998:Glycine / Q000378:metabolism*; D066298:In Vitro Techniques; D007773:Lactates; D010101:Oxygen Consumption; D010725:Phosphocreatine / Q000032:analysis; D011773:Pyruvates; D051381:Rats; D013386:Succinates
D002247:Carbon Isotopes; D005977:Glutarates; D007773:Lactates; D011773:Pyruvates; D013386:Succinates; D010725:Phosphocreatine; D000255:Adenosine Triphosphate; D005947:Glucose; D000409:Alanine; D005998:Glycine