Application of enzyme production properties in subtyping of group A streptococci according to T type.
The production of extracellular nicotinamide adenine dinucleotide glycohydrolase (NADG) and the cell-bound lipoproteinase (serum opacity reaction, SOR) by strains of different serological types of group A streptococci, in relation to the T typing, was studied. The production of both NADG and SOR, or only one of them, was found to be characteristic of serotypes, as determined by M and T antigen. No difference in the production of these enzymes was found in relation to M-positive and M-negative variants. Investigation into NADG and SOR production as related to the T type enabled the division of a single agglutination pattern into four main groups, each of which corresponds to one specific M type or more. Of the 370 strains belonging to 12 different T-agglutination patterns, 21% produced both enzymes and 42.5% failed to produce any of them, whereas the remaining 36.5% produced only one out of the two enzymes. Five streptococcal types which did not produce NADG and SOR also failed to synthesize streptolysin S at the early logarithmic phase of growth, indicating that streptolysin S production by young cultures may be also related to serotype. No correlation was found between the production of NADG-SOR as related to serotype and the production of streptolysin O, acid phosphotase, esterase, N-acetylglucosaminidase, hyaluronidase, streptokinase, and the cell-sensitizing factor. The practical and potential usefulness of NADG and SOR production in epidemiological studies is discussed.
D000372:Agglutination Tests; D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D007106:Immune Sera; D008071:Lipoprotein Lipase / Q000096:biosynthesis*; D008722:Methods; D009699:N-Glycosyl Hydrolases / Q000096:biosynthesis*; D011233:Precipitin Tests; D011817:Rabbits; D012703:Serotyping; D013291:Streptococcus / Q000145:classification* / Q000201:enzymology / Q000276:immunology / Q000378:metabolism; D013301:Streptolysins / Q000096:biosynthesis
D000942:Antigens, Bacterial; D007106:Immune Sera; D013301:Streptolysins; D008071:Lipoprotein Lipase; D009699:N-Glycosyl Hydrolases