Characterization of the A component of Streptococcus zymogenes lysin.
By utilizing conventional techniques of pressure ultrafiltration, gel filtration chromatography, diethylaminoethyl cellulose chromatography, and preparative polyacrylamide electrophoresis, the A component of the group D lysin produced by Streptococcus zymogenes has been purified to a state of apparent homogeneity when determined by the techniques of anionic and cationic disc gel electrophoresis. The A component was found to be a protein possessing a molecular weight of 27,000, a sedimentation coefficient approximating 3.2S, and a net negative charge at physiological pH.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001322:Autoanalysis; D001426:Bacterial Proteins / Q000032:analysis* / Q000302:isolation & purification; D002474:Cell-Free System; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004589:Electrophoresis, Disc; D004912:Erythrocytes / Q000276:immunology; D005374:Filtration; D006461:Hemolysis; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D011817:Rabbits; D013291:Streptococcus / Q000032:analysis*; D013301:Streptolysins / Q000032:analysis*
D000596:Amino Acids; D001426:Bacterial Proteins; D013301:Streptolysins