Amino acid sequences around the cysteine residues of rabbit muscle triose phosphate isomerase.
1. The nature of the subunits in rabbit muscle triose phosphate isomerase has been investigated. 2. Amino acid analyses show that there are five cysteine residues and two methionine residues/subunit. 3. The amino acid sequences around the cysteine residues have been determined; these account for about 75 residues. 4. Cleavage at the methionine residues with cyanogen bromide gave three fragments. 5. These results show that the subunits correspond to polypeptide chains, containing about 230 amino acid residues. The chains in triose phosphate isomerase seem to be shorter than those of other glycolytic enzymes.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002852:Chromatography, Ion Exchange; D002854:Chromatography, Paper; D003488:Cyanogen Bromide; D003545:Cysteine / Q000032:analysis; D004586:Electrophoresis; D005994:Glycerophosphates; D007535:Isomerases / Q000032:analysis*; D008715:Methionine / Q000032:analysis; D009132:Muscles / Q000201:enzymology*; D010447:Peptide Hydrolases; D010455:Peptides / Q000032:analysis; D011817:Rabbits
D000596:Amino Acids; D005994:Glycerophosphates; D010455:Peptides; D008715:Methionine; D010447:Peptide Hydrolases; D007535:Isomerases; D003545:Cysteine; D003488:Cyanogen Bromide