Improved assay of 3-hydroxy-3-methylglutaryl coenzyme A reductase.
Two improvements are described for the assay of HMG CoA reductase. These are a simple synthesis of the substrate precursor HMG-3-(14)C anhydride and a double-label ((14)C and (3)H) method for determining the amount of mevalonate-3-(14)C that is formed from the substrate.
D000429:Alcohol Oxidoreductases / Q000032:analysis*; D000812:Anhydrides; D000818:Animals; D002250:Carbon Radioisotopes; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D005069:Evaluation Studies as Topic; D005977:Glutarates; D007553:Isotope Labeling; D008722:Methods; D008798:Mevalonic Acid; D008862:Microsomes, Liver / Q000201:enzymology; D014316:Tritium; D014461:Ultracentrifugation
D000812:Anhydrides; D002250:Carbon Radioisotopes; D005977:Glutarates; D014316:Tritium; D000429:Alcohol Oxidoreductases; D008798:Mevalonic Acid