Mescaline-induced changes of brain-cortex ribosomes. Role of sperimidine in counteracting the destabilizing effect of mescaline of brain-cortex ribosomes.
1. The effect of spermidine on the mescaline-induced changes of brain-cortex ribosomes was studied by adding spermidine during the treatment of goat brain-cortex slices with mescaline. 2. Mescaline treatment of brain-cortex slices removed a portion of the endogenous spermidine from ribosomes and this removal was significantly prevented when spermidine was present during mescaline treatment. 3. Spermidine present during mescaline treatment of brain-cortex slices counteracted, to some extent, the destabilizing effect of mescaline on ribosomes with respect to heat denaturation. 4. Mescaline treatment of brain-cortex slices made ribosomes more susceptible to breakdown, releasing protein and RNA, and resulting in loss of ribosomal enzymic activities. However, spermidine present during mescaline treatment counteracted moderately the mescaline-induced ribosomal susceptibility to breakdown and ribosomal loss of enzymic activities. 5. Ribosomes of mescaline-treated cortex slices were rapidly degraded by ribonuclease and trypsin. However, if spermidine was present during mescaline treatment of brain-cortex slices the rates of degradation diminished.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002540:Cerebral Cortex / Q000187:drug effects*; D006041:Goats; D066298:In Vitro Techniques; D008635:Mescaline / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D009691:Nucleic Acid Denaturation; D011073:Polyamines / Q000494:pharmacology*; D012335:RNA, Ribosomal / Q000378:metabolism; D012260:Ribonucleases / Q000494:pharmacology; D012270:Ribosomes / Q000187:drug effects*; D013053:Spectrophotometry; D013095:Spermidine / Q000494:pharmacology; D013696:Temperature; D014357:Trypsin / Q000494:pharmacology; D014466:Ultraviolet Rays
D011073:Polyamines; D012335:RNA, Ribosomal; D012260:Ribonucleases; D014357:Trypsin; D008635:Mescaline; D013095:Spermidine