Cultural estimation of yeasts on seaweeds.
Five per cent suspensions of freshly harvested seaweeds were used as an inoculum to develop a selective medium for epiphytic yeasts. Conditions for satisfactory yeast growth and visualization as red colonies on membrane filters were obtained by supplementing a basal glucose-Trypticase-yeast extract-agar at pH 7.0 with 100 mg each of chloramphenicol and 2,3,5-triphenyl tetrazolium chloride per liter. Maximal counts were obtained by triturating the algae in prechilled (4 C) seawater with a blender for 2 to 5 min. Inhibitory phenolic materials released from phaeophytes during this process were removed with a modified Cholodny filtration. A preliminary survey indicated that yeasts were epiphytic on all nine species of seaweeds and that maximal populations occurred on the chlorophytes and rhodophytes especially during the periods of warmer water.
D000362:Agar; D002175:Candida / Q000254:growth & development / Q000302:isolation & purification; D002701:Chloramphenicol; D003470:Culture Media; D005947:Glucose; D006863:Hydrogen-Ion Concentration; D008722:Methods; D003904:Mitosporic Fungi / Q000254:growth & development / Q000302:isolation & purification; D012623:Seawater; D012624:Seaweed; D013778:Tetrazolium Salts; D014871:Water Microbiology; D015003:Yeasts / Q000254:growth & development / Q000302:isolation & purification*
D003470:Culture Media; D013778:Tetrazolium Salts; D002701:Chloramphenicol; D000362:Agar; D005947:Glucose