The conjugation of glutathione with unsaturated acyl thiol esters and the metabolic formation of S-carboxyalkylcysteines.
The partial purification and properties of an enzyme from the soluble fraction of rat liver that catalyses the reaction of glutathione with 2,3-unsaturated acyl thiol esters is described, and its possible role in the formation of S-carboxyalkylcysteines is discussed. The synthesis of S-(3-methylcrotonyl)- and S-(2-methylcrotonyl)-N-acetylcysteamine and of S-crotonyl-NN-dimethylcysteamine hydrochloride and dicyclohexylammonium S-crotonyl-N-acetyl-l-cysteine is described.
D000475:Alkenes; D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D003545:Cysteine / Q000096:biosynthesis* / Q000138:chemical synthesis; D004952:Esters; D005978:Glutathione / Q000032:analysis; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D051381:Rats; D013438:Sulfhydryl Compounds; D014166:Transferases / Q000302:isolation & purification
D000475:Alkenes; D004952:Esters; D013438:Sulfhydryl Compounds; D014166:Transferases; D005978:Glutathione; D003545:Cysteine