A new method using 'proteinase K' to prevent mRNA degradation during isolation from HeLa cells.
D002254:Carbonates; D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D002621:Chemistry; D000431:Ethanol; D006367:HeLa Cells / Q000032:analysis*; D006868:Hydrolysis; D007700:Kinetics; D008722:Methods; D010447:Peptide Hydrolases; D012313:RNA; D012333:RNA, Messenger / Q000096:biosynthesis / Q000302:isolation & purification*; D012335:RNA, Ribosomal / Q000302:isolation & purification; D012260:Ribonucleases / Q000037:antagonists & inhibitors; D013696:Temperature; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D015003:Yeasts / Q000201:enzymology
D002254:Carbonates; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D014316:Tritium; D000431:Ethanol; D012313:RNA; D012260:Ribonucleases; D010447:Peptide Hydrolases; D014529:Uridine