Structural proteins of adenovirus-associated virus type 3.
Three major structural proteins were found in adenovirus-associated virus (AAV) type 3H virions which were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The molecular weights of the polypeptides were determined to be approximately 66,000 (VP1), 80,000 (VP2), and 92,000 (VP3). The component having a molecular weight of 66,000 comprised about 80% of the total virion protein in the major AAV-3H particle, and the other two components comprised about 10% each. Proteins of the same molecular weight were found in the minor dense AAV-3H virion, but the 80,000- and 92,000-molecular-weight components were present at about one-half the concentration. The AAV-3H virion contains about 72 molecules of VP1 and 8 and 7 molecules of VP2 and VP3, respectively.
D000256:Adenoviridae / Q000032:analysis* / Q000254:growth & development; D002277:Carcinoma; D002460:Cell Line; D004229:Dithiothreitol; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008970:Molecular Weight; D009062:Mouth Neoplasms; D010455:Peptides / Q000032:analysis; D012526:Satellite Viruses / Q000032:analysis* / Q000254:growth & development; D012703:Serotyping; D012967:Sodium Dodecyl Sulfate; D014764:Viral Proteins / Q000032:analysis*
D010455:Peptides; D014764:Viral Proteins; D012967:Sodium Dodecyl Sulfate; D004229:Dithiothreitol