The nucleotide sequences of some large ribonuclease T 1  products from bacteriophage R17 ribonucleic acid.
A method of ;fingerprinting' high-molecular-weight (32)P-labelled RNA species, using a two-dimensional thin-layer-chromatographic separation of ribonuclease T(1) digestion products, has been applied to RNA from the Escherichia coli bacteriophage R17. The ;fingerprinting' technique, besides giving a unique pattern that can be used as a characterization of the RNA, has made it possible to isolate a number of the larger oligonucleotides and to determine their nucleotide sequences.
D001345:Autoradiography; D001483:Base Sequence; D002855:Chromatography, Thin Layer; D003090:Coliphages / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D009711:Nucleotides / Q000032:analysis*; D010759:Phosphorus Isotopes; D011119:Polynucleotides / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis*; D012260:Ribonucleases
D009711:Nucleotides; D010759:Phosphorus Isotopes; D011119:Polynucleotides; D012367:RNA, Viral; D012260:Ribonucleases