Identification of prostaglandins in prevertebral venous blood after preganglionic stimulation of the cat superior cervical ganglion.
1. In four out of seven experiments, prostaglandin-like activity was detected in prevertebral venous blood from the superior cervical ganglion, collected during and after preganglionic nerve stimulation.2. Prostaglandins E(1), E(2), F(2alpha) and a prostaglandin A were identified using solvent partition, column and thin-layer chromatography and bioassay techniques.3. The release of PGF(2alpha) was confirmed by combined gas chromatography-mass spectrometry.
D000818:Animals; D001681:Biological Assay; D001794:Blood Pressure; D002415:Cats; D002845:Chromatography; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D003377:Countercurrent Distribution; D004558:Electric Stimulation; D005260:Female; D005725:Ganglia, Autonomic / Q000098:blood supply / Q000378:metabolism*; D007082:Ileum / Q000187:drug effects; D007583:Jejunum / Q000187:drug effects; D013058:Mass Spectrometry; D011453:Prostaglandins / Q000097:blood*; D011817:Rabbits; D051381:Rats; D013270:Stomach / Q000187:drug effects; D014132:Trachea / Q000187:drug effects; D014599:Uterus / Q000187:drug effects; D014680:Veins
D011453:Prostaglandins