Selective precipitation of ribonucleic acid from a mixture of total cellular nucleic acids extracted from cultured mammalian cells.
A simple and reproducible method is described for precipitating RNA selectively from total mammalian-cell nucleic acids extracted by the phenol-sodium dodecyl sulphate procedure at pH8.0. Under specified conditions bulk RNA is precipitated almost quantitatively whereas bulk DNA remains in solution. Minor components of RNA (detected by pulse-labelling and chromatography on methylated albumin-kieselguhr) and rapidly labelled components of DNA containing single-stranded regions are also precipitated. The usefulness of the method is discussed in the context of isolating separately both RNA and DNA from cultured cells that are difficult to obtain in quantity.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor; D002478:Cells, Cultured / Q000032:analysis*; D011232:Chemical Precipitation; D002845:Chromatography; D004247:DNA / Q000096:biosynthesis / Q000302:isolation & purification; D003902:Detergents; D004622:Embryo, Mammalian; D005347:Fibroblasts; D007739:L Cells; D008722:Methods; D051379:Mice; D009696:Nucleic Acids / Q000032:analysis*; D010636:Phenols; D012313:RNA / Q000302:isolation & purification*; D012996:Solutions
D003902:Detergents; D009696:Nucleic Acids; D010636:Phenols; D012996:Solutions; D012313:RNA; D004247:DNA