The synthesis of a self-propagating and infectious nucleic acid with a purified enzyme.
D003090:Coliphages / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D009713:Nucleotidyltransferases / Q000378:metabolism*; D011523:Protoplasts; D012367:RNA, Viral / Q000096:biosynthesis*