Evidence for intergenic complementation in hybrid cells derived from two human diploid strains each carrying an X-linked mutation.
Two male diploid fibroblast strains, each carrying deficiency mutations at different X-linked loci (glucose-6-phosphate dehydrogenase and hypoxanthine-guanine-phosphoribosyltransferase) have been successfully hybridized. The resulting mononucleated hybrid cells have been shown to synthesize both normal gene products, indicating that both X chromosomes are functionally active in the hybrid cells. We believe this is the first reported example of intergenic complementation in fused human diploid cells.
D001345:Autoradiography; D002875:Chromosomes; D046508:Culture Techniques; D003582:Cytogenetics; D004171:Diploidy; D005260:Female; D005347:Fibroblasts; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000235:genetics*; D006801:Humans; D006824:Hybridization, Genetic; D008607:Intellectual Disability / Q000235:genetics; D008297:Male; D008722:Methods; D010428:Pentosephosphates; D010641:Phenotype; D011687:Purines; D012728:Sex Chromatin; D012730:Sex Chromosomes; D012867:Skin / Q000166:cytology; D013936:Thymidine; D014166:Transferases; D014316:Tritium
D010428:Pentosephosphates; D011687:Purines; D014316:Tritium; D014166:Transferases; D013936:Thymidine