Precipitation of proteins by vinblastine and calcium ions.
We have found that vinblastine sulfate can precipitate a number of proteins in addition to microtubule protein. Those proteins which precipitate with vinblastine sulfate, all of which were derived from structure, also precipitate with Ca(2+) ions. Our results suggest that vinblastine sulfate, presumably acting as a cation, precipitates proteins by combining with sites which can also combine with Ca(2+) ions.
D000818:Animals; D001798:Blood Proteins; D001923:Brain Chemistry; D002118:Calcium; D002127:Calcium Isotopes; D011232:Chemical Precipitation; D002642:Chick Embryo; D002645:Chickens; D003078:Colchicine; D004912:Erythrocytes; D007202:Indicators and Reagents; D007782:Lactoglobulins / Q000302:isolation & purification; D008870:Microtubules / Q000032:analysis; D009113:Muramidase / Q000302:isolation & purification; D009124:Muscle Proteins / Q000302:isolation & purification; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D009940:Organoids / Q000032:analysis*; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000302:isolation & purification; D014316:Tritium; D014747:Vinblastine
D001798:Blood Proteins; D002127:Calcium Isotopes; D007202:Indicators and Reagents; D007782:Lactoglobulins; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D014316:Tritium; D012710:Serum Albumin, Bovine; D014747:Vinblastine; D009113:Muramidase; D003078:Colchicine; D002118:Calcium