Labeling and other effects of actinomycin D on human chromosomes.
(3)H-actinomycin D, a guanine-binding agent, labels fixed human chromosomes nonrandomly. Actinomycin D added in G2 inhibits secondary constrictions and breaks chromosomes. There is some tendency for label to be concentrated at the ends of chromosomes and near the centromere. Labeling with (3)H-thymidine in the late stage of DNA synthesis shows a different pattern and in general lacks the telomeric concentrations. The sites of actinomycin D-induced breaks do not show good correspondence with the sites of actinomycin D label.
D002875:Chromosomes / Q000187:drug effects*; D002900:Chromosomes, Human, 1-3 / Q000187:drug effects; D002901:Chromosomes, Human, 13-15 / Q000187:drug effects; D002902:Chromosomes, Human, 16-18 / Q000187:drug effects; D002903:Chromosomes, Human, 19-20 / Q000187:drug effects; D002904:Chromosomes, Human, 21-22 and Y / Q000187:drug effects; D002905:Chromosomes, Human, 4-5 / Q000187:drug effects; D002906:Chromosomes, Human, 6-12 and X / Q000187:drug effects; D004261:DNA Replication; D003609:Dactinomycin / Q000494:pharmacology*; D005347:Fibroblasts; D006801:Humans; D008214:Lymphocytes; D012730:Sex Chromosomes / Q000187:drug effects; D013936:Thymidine; D014316:Tritium
D014316:Tritium; D003609:Dactinomycin; D013936:Thymidine