An in vitro system that simulates plant cell extension growth.
A new technique is described for studying controlled in vitro extension of plant cell walls. This technique uses frozen-thawed Avena coleoptile segments in which turgor is replaced by a constant applied force. In this system rapid extension is induced by low pH (3.0-3.6) but not by auxins or CO(2). Extensions of over 30% have been achieved. Our results indicate that cell wall synthesis is not required for rapid cell extension, and suggest that wall extension may be controlled by acid-labile, alkali-stable cell wall bonds.
D002473:Cell Wall / Q000254:growth & development*; D002523:Edible Grain; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008954:Models, Biological; D059828:Plant Cells; D063245:Plant Development; D010937:Plant Growth Regulators / Q000494:pharmacology
D010937:Plant Growth Regulators