Immunoglobulin synthesis and secretion. 3. Incorporation of glucosamine into immunoglobulin on polyribosomes.
Mouse myeloma cells were pulse-labeled in vitro with (3)H-D-glucosamine and (14)C-L-leucine. Analysis on sucrose gradients revealed incorporation of both isotopes into polyribosomes and release of most of such radioactivity after treatment of labeled cells in vitro with puromycin. A mixing experiment excluded the in vitro binding to unlabeled polyribosomes of (3)H-glucosamine in labeled post-ribosomal material. Polyribosomes labeled with (3)H-glucosamine were precipitated with antiserum specific for mouse immunoglobulin. By chromatography and gel filtration, the precipitable radioactivity was shown to be glucosamine covalently bound to heavy and light chains.
D000818:Animals; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D005944:Glucosamine / Q000378:metabolism*; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007930:Leucine / Q000378:metabolism; D051379:Mice; D009101:Multiple Myeloma / Q000378:metabolism*; D009374:Neoplasms, Experimental / Q000378:metabolism; D011485:Protein Binding; D012270:Ribosomes / Q000378:metabolism*; D014316:Tritium
D002247:Carbon Isotopes; D007074:Immunoglobulin G; D014316:Tritium; D007930:Leucine; D005944:Glucosamine