Dinucleotides as products of an exonucleolytic activity association with the Micrococcus luteus DNA polymerase.
The partially purified DNA polymerase from Micrococcus luteus contains a low level of exonucleolytic activity. The enzyme preparation (1200-fold purified) contains approximately 100 times more polymerase than exonuclease activity. Both single- and double-stranded DNA are degraded at the same rate. The predominant products are dinucleoside diphosphates (d-pXpY); mononucleotides and a trace of trinucleotides are also produced. Each of these products is formed at a constant rate throughout the course of the reaction. The nuclease degrades a DNA chain from the 5'-end. The enzyme preparation contains no detectable endonuclease activity.
D002247:Carbon Isotopes; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004269:DNA, Bacterial; D003851:Deoxyribonucleases / Q000378:metabolism*; D007700:Kinetics; D008837:Micrococcus / Q000201:enzymology*; D009711:Nucleotides / Q000032:analysis*; D010759:Phosphorus Isotopes; D011119:Polynucleotides; D014316:Tritium
D002247:Carbon Isotopes; D004269:DNA, Bacterial; D009711:Nucleotides; D010759:Phosphorus Isotopes; D011119:Polynucleotides; D014316:Tritium; D004254:DNA Nucleotidyltransferases; D003851:Deoxyribonucleases