Genes for neuronal properties expressed in neuroblastoma x L cell hybrids.
Neuroblastoma cells with electrically excitable membranes were fused with electrically passive L cells having a hitherto undescribed electrical marker. Hybrid cells, examined 10-40 generations after fusion, were found to be electrically excitable. The results show that at least a part of the genetic information for neuron differentiation can be functionally expressed in N x L hybrid cells. Evidence for the regulation of action potential components was also found.
D000200:Action Potentials; D000630:Aminopterin / Q000494:pharmacology; D000818:Animals; D002454:Cell Differentiation; D002462:Cell Membrane / Q000502:physiology; D004558:Electric Stimulation; D005796:Genes; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis; D006824:Hybridization, Genetic; D066298:In Vitro Techniques; D007739:L Cells / Q000502:physiology*; D008564:Membrane Potentials; D051379:Mice; D009154:Mutation; D009447:Neuroblastoma / Q000503:physiopathology*; D009474:Neurons / Q000166:cytology*; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism
D012964:Sodium; D005956:Glucose-6-Phosphate Isomerase; D000630:Aminopterin; D011188:Potassium