Stereochemical analysis of the specificity of pancreatic RNAse with polyformycin as substrate: differentiation of the transphosphorylation and hydrolysis reactions.
A stereochemical analysis of the substrate and inhibitor specificities of bovine pancreatic ribonuclease A is presented. A scheme is proposed in which the binding specificity for this protein-nucleic acid interaction is rationalized in terms of a simple system of H-bonds. The functional groups that govern substrate binding for transphosphorylation and hydrolysis, respectively, are considered and differentiated, and predictions are offered concerning the interaction of presumptive substrates with RNase.
D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D006859:Hydrogen; D009711:Nucleotides; D010179:Pancreas / Q000201:enzymology; D010758:Phosphorus; D011743:Pyrimidines; D012260:Ribonucleases; D013237:Stereoisomerism
D009711:Nucleotides; D011743:Pyrimidines; D010758:Phosphorus; D006859:Hydrogen; D012260:Ribonucleases