Giant nucleoplasmic RNA in the switch-on of compensatory renal growth.
Heterogeneous RNA (HnRNA) (ca. 10S-70S) labeled for ten minutes with (3)H-uridine was identified by polyacrylamide-gel electrophoresis in the nucleoplasm of kidney cells. In the region >28S several discretely migrating species were present. Ten minutes after the removal of one kidney, labeled HnRNA >28S ("giant" HnRNA) in the remaining kidney began to decrease, and by 60 minutes it had fallen drastically. The presence of labeled giant HnRNA during a pulse label after uninephrectomy and its disappearance after longer labeling suggest that the decrease is a consequence of faster processing. Accelerated processing of giant HnRNA is antecedent to the increased synthesis of renal cytoplasmic RNA that follows uninephrectomy.
D000818:Animals; D002467:Cell Nucleus; D003593:Cytoplasm; D004586:Electrophoresis; D005782:Gels; D007668:Kidney / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D008297:Male; D008722:Methods; D051379:Mice; D009392:Nephrectomy; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification*; D014316:Tritium; D014529:Uridine
D005782:Gels; D014316:Tritium; D012313:RNA; D014529:Uridine