Molecular hybridization of radioactive DNA to the DNA of cytological preparations.
A method is presented for detecting the cellular location of specific DNA fractions. The technique involves the hybridization of a radioactive test DNA in solution to the stationary DNA of a cytological preparation. Sites of DNA binding are then detected by autoradiography. Experiments with DNA of the toad Xenopus are described.
D000818:Animals; D001345:Autoradiography; D004247:DNA; D005260:Female; D006824:Hybridization, Genetic; D008722:Methods; D051379:Mice; D010063:Ovum; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D014316:Tritium; D004247:DNA; D013936:Thymidine