Immunoglobulin synthesis and secretion, IV. Sites of incorporation of sugars as determined by subcellular fractionation.
P(3)K cells were uniformly labeled with [(14)C]leucine and also for 1 hr with [(3)H]leucine, [(3)]galactose, or [(3)H]glucosamine. Subcellular fractionation of labeled cells was performed and the amounts of radioactive immunoglobulin G in each fraction were determined. The results indicate that amino acids are incorporated in the rough endoplasmic reticulum, galactose primarily in the smooth endoplasmic reticulum, and glucosamine at both sites. A small portion of radioactivity was found in the microsomal supernatant regardless of the radioisotope employed.
D000818:Animals; D002247:Carbon Isotopes; D002460:Cell Line / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D004721:Endoplasmic Reticulum / Q000032:analysis / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism*; D005944:Glucosamine / Q000378:metabolism*; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007136:Immunoglobulins / Q000096:biosynthesis; D066298:In Vitro Techniques; D007930:Leucine / Q000378:metabolism*; D051379:Mice; D008861:Microsomes / Q000032:analysis / Q000378:metabolism*; D009101:Multiple Myeloma / Q000378:metabolism* / Q000662:veterinary; D009363:Neoplasm Proteins / Q000096:biosynthesis; D014316:Tritium; D014633:Valine / Q000378:metabolism
D002247:Carbon Isotopes; D007074:Immunoglobulin G; D007136:Immunoglobulins; D009363:Neoplasm Proteins; D014316:Tritium; D007930:Leucine; D014633:Valine; D005944:Glucosamine; D005690:Galactose