Esterolytic activity of elastase.
The esters of L-alanine were found to be excellent substrates for elastase. The pH-dependence study using two methyl esters of this substrate showed the participation in the mechanism of an ionizing group with an apparent pK of 6.85 and a decrease of the activity at pH's greater than 10 (that could correspond to an apparent pK of 11.4).
D000409:Alanine / Q000378:metabolism*; D000818:Animals; D004952:Esters / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D010196:Pancreatic Elastase / Q000378:metabolism*; D012995:Solubility; D013552:Swine
D004952:Esters; D010196:Pancreatic Elastase; D000409:Alanine