A genetic assay for mRNA's of phage T4.
A method has been developed by which many gene-specific mRNA's in T4-infected cells can be quantitatively assayed. The method involves separation of complementary strands of phage T4 DNA, hybridization of the strands with RNA, digestion of nonhybridized regions of DNA with an endonuclease specific for single-stranded DNA, and assay of protected genetic markers by transformation. It has been shown that the gene gammaIIB is transcribed early from the light strand and that the gene 21 is transcribed late from the heavy strand.
D003090:Coliphages / Q000032:analysis*; D005815:Genetic Code; D006824:Hybridization, Genetic; D008722:Methods; D012333:RNA, Messenger / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis; D014170:Transformation, Genetic
D012333:RNA, Messenger; D012367:RNA, Viral