Immunochemical measurement of double-stranded RNA of uninfected and arbovirus-infected mammalian cells.
A quantitative complement fixation assay which specifically measures double-stranded RNA has been used to study this RNA extracted from uninfected and arbovirus-infected cells. The double-straned RNA of the uninfected BHK-21 cells sedimented in the 12S region in sucrose gradients. The double-stranded RNA of Sinbis virus-infected cells, as measured immunochemically, included a predominant peak at 12S, a smaller 18S peak, and polydisperse material extending into the 26-30S region. All classes of this RNA detected immunochemically showed a sharp thermal denaturation curve, and increased in amount progressively during infection, with the 12S peak predominant at all times.
D000818:Animals; D002247:Carbon Isotopes; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D003168:Complement Fixation Tests; D006224:Cricetinae; D003588:Cytopathogenic Effect, Viral; D003716:Dengue Virus / Q000378:metabolism / Q000472:pathogenicity; D004668:Encephalitis Viruses / Q000378:metabolism* / Q000472:pathogenicity; D006358:Hot Temperature; D007668:Kidney; D009691:Nucleic Acid Denaturation; D012367:RNA, Viral / Q000032:analysis*; D014529:Uridine / Q000378:metabolism
D002247:Carbon Isotopes; D012367:RNA, Viral; D014529:Uridine