Production of 19S IgM antibodies with restricted heterogeneity from sharks.
Two of three nurse sharks, Ginglymostoma cirratum, immunized with streptococcal A-variant vaccine, produced over 7 mg/ml serum of 19S IgM antibody to the group-specific carbohydrate. The light chains of this specific antibody from one shark were separated into multiple bands by disc electrophoresis. The light chains from the other antibody-producing animal were much less heterogeneous and gave only one predominant band.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000917:Antibody Formation; D000918:Antibody Specificity; D001428:Bacterial Vaccines; D002850:Chromatography, Gel; D004589:Electrophoresis, Disc; D007075:Immunoglobulin M / Q000096:biosynthesis*; D007136:Immunoglobulins / Q000032:analysis / Q000096:biosynthesis; D011233:Precipitin Tests; D012754:Sharks / Q000276:immunology*; D013291:Streptococcus / Q000276:immunology; D014461:Ultracentrifugation
D000906:Antibodies; D001428:Bacterial Vaccines; D007075:Immunoglobulin M; D007136:Immunoglobulins