Electrophoretically homogeneous anti-DNP antibodies with restricted isoelectric points elicited in mice by immunization with the antigen papain-S-DNPL.
The primary immunization of outbred mice with the antigen Pap-S-DNPL results in the generation of low titers of anti-DNP antibody which in about one-third of the responding animals is as homogeneous as a myeloma protein by the combined criteria of (a) isoelectric focusing in gels and (b) gel electrophoresis of the antibody light chains. The electrophoretically homogeneous antibodies show a marked restriction of isoelectric points near pH 5.0. Such marked selectivity of the antihapten antibodies appears to result from the chemical and structural homogeneity of the Pap-S-DNPL antigen.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D004140:Dinitrophenols / Q000494:pharmacology*; D004589:Electrophoresis, Disc; D006241:Haptens / Q000032:analysis; D007114:Immunization; D051379:Mice; D010206:Papain; D012510:Sarcoma 180 / Q000276:immunology
D000906:Antibodies; D004140:Dinitrophenols; D006241:Haptens; D010206:Papain