Identification and origin of some chloroplast aminoacyl-tRNA synthetases and tRNAs.
Light-grown, wild type Euglena gracilis contains two aminoacyl-tRNA synthetases for both phenylalanine and isoleucine. Only one of the two synthetases for each amino acid is found in isolated chloroplasts, as are the light-induced phenylalanine and isoleucine tRNAs. In each case the light-induced chloroplast tRNAs can only be acylated by the chloroplast synthetases. The chloroplast isoleucyl-tRNA synthetase is light-inducible and cannot be detected in dark-grown cells or in cells of the bleached mutant W(3)BUL. The presence of the chloroplast phenylalanyl-tRNA synthetase in W(3)BUL, which contains no chloroplast DNA or structure, indicates that this chloroplast enzyme is synthesized in the cytoplasm and is coded by nuclear genes.
D000596:Amino Acids; D002736:Chloroplasts / Q000201:enzymology*; D002848:Chromatography, DEAE-Cellulose; D004790:Enzyme Induction; D005055:Euglena; D005815:Genetic Code; D008027:Light; D012316:RNA Nucleotidyltransferases / Q000032:analysis*; D012343:RNA, Transfer / Q000032:analysis*
D000596:Amino Acids; D012343:RNA, Transfer; D012316:RNA Nucleotidyltransferases