Evidence for a single-stranded adenovirus-associated virus genome: formation of a DNA density hybrid on release of viral DNA.
Extracted adenovirus-associated virus DNA is known to be double-stranded, and, therefore, it has been assumed that these virus particles contain a double-stranded genome. Recent findings, however, have suggested that the DNA in virus particles is equivalent to only half the molecular weight of extracted molecules. A density analysis of DNA extracted from a mixture of virus particles containing either bromodeoxyuridine-substituted or unsubstituted DNA shows that virions contain single-stranded DNA which, when released, forms duplex structures. A similar circumstance is as yet unknown among other viruses.
D000256:Adenoviridae; D001973:Bromodeoxyuridine; D002499:Centrifugation, Density Gradient; D002501:Centrifugation, Zonal; D004279:DNA, Viral / Q000032:analysis; D006824:Hybridization, Genetic; D012526:Satellite Viruses; D013046:Specific Gravity
D004279:DNA, Viral; D001973:Bromodeoxyuridine