Release of trapped marker from liposomes by the action of purified complement components.
Liposomes containing trapped glucose marker were prepared from the chloroform-soluble fraction of sheep erythrocyte membranes. These liposomes release glucose when incubated with rabbit anti-sheep erythrocyte serum and a source of complement. Experiments with purified human complement components show that loss of marker is absolutely dependent on the presence of components 2 and 8. An absolute requirement for component 9 cannot be demonstrated, although it stimulates glucose release from the liposomes. These results establish a parallelism between the response of biological membranes and liposomal membranes to antibody and complement.
D000818:Animals; D002462:Cell Membrane; D003165:Complement System Proteins / Q000494:pharmacology*; D004912:Erythrocytes; D005947:Glucose / Q000378:metabolism; D008055:Lipids; D012756:Sheep
D008055:Lipids; D003165:Complement System Proteins; D005947:Glucose