Serine requirement in leukemic and normal blood cells.
In a serine-deficient medium, cells from humangt chronic granulocytic leukemia and normal human bone marrow exhibited a marked inhibition of incorporation of-radioactive precursors of DNA, RNA, and protein into an acid insoluble cell fraction. Normal diploid human fibroblasts did not exhibit inhibition without serine. Chronic granulocytic leukemia and normal marrow cells were essentially unable to synthesize C(14)-serine from C(14)-glucose, while human diploid fibroblasts were highly capable of this synthesis.
D000409:Alanine / Q000378:metabolism; D001216:Asparagine / Q000378:metabolism; D001224:Aspartic Acid / Q000378:metabolism; D001853:Bone Marrow / Q000378:metabolism*; D001854:Bone Marrow Cells; D002247:Carbon Isotopes; D003470:Culture Media; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D005347:Fibroblasts / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007951:Leukemia, Myeloid / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D011392:Proline / Q000378:metabolism; D012334:RNA, Neoplasm / Q000096:biosynthesis*; D012694:Serine / Q000096:biosynthesis / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D002247:Carbon Isotopes; D003470:Culture Media; D004273:DNA, Neoplasm; D005971:Glutamates; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; D014316:Tritium; D001224:Aspartic Acid; D012694:Serine; D001216:Asparagine; D011392:Proline; D005947:Glucose; D000409:Alanine; D005998:Glycine; D013936:Thymidine; D014529:Uridine