A thrombin-sensitive protein of human platelet membranes.
The action of thrombin on intact human platelets has been studied with the aid of polyacrylamide gel electrophoresis in sodium dodecylsulfate. A single major membrane protein band with a molecular weight of 190,000 disappears after thrombin treatment, while a new membrane protein with a molecular weight of 107,000 appears. This may represent hydrolysis of the thrombin-sensitive protein. When platelets are disrupted or when the thrombin-sensitive protein is solubilized from membranes prior to thrombin treatment, no hydrolysis occurs. The effect of thrombin on the platelet membrane protein is complete within 2 min which suggests that hydrolysis of this membrane protein may trigger the physiological effects of thrombin on platelets.
D001792:Blood Platelets / Q000187:drug effects*; D001798:Blood Proteins / Q000032:analysis*; D003902:Detergents; D004589:Electrophoresis, Disc; D006801:Humans; D008566:Membranes / Q000187:drug effects; D008970:Molecular Weight; D012995:Solubility; D013464:Sulfuric Acids; D013917:Thrombin / Q000494:pharmacology*
D001798:Blood Proteins; D003902:Detergents; D013464:Sulfuric Acids; D013917:Thrombin