Isolation of a chloramphenicol-resistant protein from lambda-infected cells.
During infection with bacteriophage varphiX174, a protein, made in the presence of 30 gamma chloramphenicol/ml but not made in the presence of concentrations greater than 100 gamma/ml, has been shown to be essential for viral DNA replication. A protein with similar chromatographic properties and "chloramphenicol resistance" has now been demonstrated in cells infected with lambda bacteriophage.
D002247:Carbon Isotopes; D002701:Chloramphenicol / Q000494:pharmacology*; D002845:Chromatography; D003090:Coliphages; D004261:DNA Replication; D007930:Leucine; D008722:Methods; D014316:Tritium; D014764:Viral Proteins / Q000096:biosynthesis / Q000302:isolation & purification*
D002247:Carbon Isotopes; D014764:Viral Proteins; D014316:Tritium; D002701:Chloramphenicol; D007930:Leucine