Isolation of temperature-sensitive mutants of L-cells.
Procedures are described for the isolation of conditional lethal mutants of mouse L-60T cells. The mutant lines were temperature sensitive by the following criteria: (a) colony-forming ability, (b) growth in suspension culture, and (c) rate of uptake of tritiated-thymidine.
D000818:Animals; D002999:Clone Cells / Q000254:growth & development; D046508:Culture Techniques; D007739:L Cells / Q000254:growth & development / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D051379:Mice; D009154:Mutation; D013696:Temperature; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D014316:Tritium; D008239:Lysine; D013936:Thymidine; D014529:Uridine