The electron transport system in nitrogen fixation by Azotobacter. I. Azotoflavin as an electron carrier.
A flavoprotein, named azotoflavin, was isolated from an extract of Azotobacter vinelandii cells, which linked the reducing power generated by illuminated spinach chloroplasts to the Azotobacter nitrogen-fixing enzyme complex. The photoreduction of the yellow azotoflavin by chloroplasts produced a stable, free-radical semiquinone, blue in color, with properties similar to those described by other investigators for an Azotobacter flavoprotein of unknown biological function.
D001395:Azotobacter / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism / Q000528:radiation effects; D002848:Chromatography, DEAE-Cellulose; D004579:Electron Transport; D005415:Flavins / Q000302:isolation & purification / Q000378:metabolism*; D005609:Free Radicals / Q000378:metabolism; D008027:Light; D009586:Nitrogen Fixation; D010088:Oxidoreductases / Q000378:metabolism*; D011830:Radiation Effects; D013447:Sulfites / Q000378:metabolism
D005415:Flavins; D005609:Free Radicals; D013447:Sulfites; D010088:Oxidoreductases