Isolation of microtubule protein from cultured mouse neuroblastoma cells.
The addition of vinblastine to high-speed supernatants derived from homogenates of cultured mouse neuroblastoma cells results in the formation of a precipitate which has been characterized as microtubule protein by the following criteria: colchicine-binding activity, molecular weight, amino acid composition, and electrophoretic mobility. The method therefore permits the rapid isolation of microtubule protein from crude supernatants of neuroblastoma cells.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002498:Centrifugation; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D003078:Colchicine; D046508:Culture Techniques; D004589:Electrophoresis, Disc; D056890:Eukaryota; D005407:Flagella; D051379:Mice; D008938:Mitosis; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000032:analysis / Q000302:isolation & purification*; D009374:Neoplasms, Experimental; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000302:isolation & purification*; D009447:Neuroblastoma; D009940:Organoids / Q000032:analysis*; D011485:Protein Binding; D011506:Proteins; D014316:Tritium; D014747:Vinblastine
D000596:Amino Acids; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins; D014316:Tritium; D014747:Vinblastine; D003078:Colchicine