Antibodies to a unique region in lysozyme provoked by a synthetic antigen conjugate.
A synthetic conjugate, prepared by covalent binding of a lysozyme fragment (sequence 64-83, denoted "loop" peptide) to a synthetic branched polypeptide, elicited in rabbits the formation of antibodies with specificity directed against a unique region in native lysozyme. These anti-"loop" antibodies were isolated immunospecifically on a lysozyme-cellulose immunoadsorbent. Antibodies with a similar specificity were isolated from antilysozyme sera with an immunoadsorbent prepared from the same "loop" peptide. The capacity of the anti-"loop" antibodies to distinguish between the "loop" peptide, containing a disulfide bridge, and the open-chain peptide derived from it suggests that they are directed against a conformation-dependent determinant.
D000818:Animals; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D009113:Muramidase / Q000037:antagonists & inhibitors*; D011817:Rabbits; D013440:Sulfides
D013440:Sulfides; D009113:Muramidase