DNA- dependent RNA-directed protein synthesis in vitro. I. Extent of genome transcription.
We have described an in vitro system which couples UTP incorporation into RNA by DNA-dependent RNA polymerase with RNA-directed amino acid incorporation into polypeptides. Using the technique of DNA-RNA hybridization, we have demonstrated that the whole PhiX-174 genome is transcribed during concomitant protein synthesis.
D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D003090:Coliphages; D004279:DNA, Viral; D005815:Genetic Code; D006824:Hybridization, Genetic; D066298:In Vitro Techniques; D007700:Kinetics; D012316:RNA Nucleotidyltransferases / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*; D014316:Tritium; D014500:Uracil Nucleotides / Q000378:metabolism; D014633:Valine / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis*
D002247:Carbon Isotopes; D004279:DNA, Viral; D012367:RNA, Viral; D014500:Uracil Nucleotides; D014764:Viral Proteins; D014316:Tritium; D012316:RNA Nucleotidyltransferases; D014633:Valine