Evidence for one functional phenylalanyl-tRNA binding site on the 30S ribosomal subunit.
NH(2) terminal analysis of polyphenylalanine formed from 30S subunit-bound (14)C-phenylalanyl-tRNA suggests that there is only one site in a 30S subunit for specific binding of phenylalanyl-tRNA. Assuming that no movement of the bound phenylalanyl-tRNA takes place during association of 30S subunits with 50S ribosomal subunits, the binding site of the 30S ribosomal subunit corresponds to site 2 (-NH(2) side with respect to the growing polypeptide chain) of the two binding sites of 70S ribosomes.
D001665:Binding Sites; D002247:Carbon Isotopes; D008274:Magnesium / Q000494:pharmacology; D010649:Phenylalanine; D011188:Potassium / Q000494:pharmacology; D012343:RNA, Transfer / Q000378:metabolism*; D012270:Ribosomes
D002247:Carbon Isotopes; D010649:Phenylalanine; D012343:RNA, Transfer; D008274:Magnesium; D011188:Potassium