Stimulation of glucose transport in cultures of density-inhibited chick embryo cells.
The rate of glucose transport in sparse, rapidly growing chick-embryo fibroblasts is much greater than that in density-inhibited cells. The addition of fresh chicken serum or trypsin to the medium of density-inhibited cells causes a large increase in the rate of glucose transport that is detectable 15 min after addition. The increase in glucose transport precedes the increase in DNA synthesis by 5-6 hr. Only small changes in rates of transport are seen with nucleosides or a nonmetabolizable amino acid. The increase in glucose transport requires protein synthesis but not RNA or DNA synthesis.
D000621:Aminoisobutyric Acids / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006128:Growth / Q000187:drug effects; D006601:Hexoses / Q000378:metabolism; D007106:Immune Sera / Q000494:pharmacology; D007930:Leucine / Q000378:metabolism; D014176:Protein Biosynthesis; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014357:Trypsin / Q000494:pharmacology; D014529:Uridine / Q000378:metabolism
D000621:Aminoisobutyric Acids; D006601:Hexoses; D007106:Immune Sera; D014316:Tritium; D003609:Dactinomycin; D003513:Cycloheximide; D014357:Trypsin; D007930:Leucine; D005947:Glucose; D013936:Thymidine; D014529:Uridine