Synthesis of   and   chains of rabbit hemoglobin in a cell-free extract from Krebs II ascites cells.
An RNA sedimenting at 10 S, with a molecular weight of 2.3 x 10(5), was isolated from rabbit reticylocyte polyribosomes. When this RNA is added to a cell-free extract from Krebs II ascites cells, both alpha and beta chains of rabbit hemoglobin are synthesized; beta chains are made in about 50% excess over alpha chains.
D000818:Animals; D002247:Carbon Isotopes; D002287:Carcinoma, Krebs 2 / Q000378:metabolism*; D002474:Cell-Free System; D002845:Chromatography; D004589:Electrophoresis, Disc; D004590:Electrophoresis, Paper; D005815:Genetic Code; D005914:Globins / Q000096:biosynthesis; D006454:Hemoglobins / Q000032:analysis / Q000096:biosynthesis*; D007930:Leucine / Q000378:metabolism; D051379:Mice; D010455:Peptides / Q000032:analysis; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D014316:Tritium; D014443:Tyrosine / Q000378:metabolism
D002247:Carbon Isotopes; D006454:Hemoglobins; D010455:Peptides; D012333:RNA, Messenger; D014316:Tritium; D014443:Tyrosine; D005914:Globins; D007930:Leucine