Initiation of DNA synthesis in cell cultures by colcemid.
Mitotic inhibitors (colcemid, colchicine, and vinblastine) initiate DNA synthesis in dense stationary cultures of mouse embryo fibroblast-like cells. This initiation is not due to any changes in local cell population density. Relationships between the activation of proliferation and other changes of interphase fibroblast-like cells produced by the mitotic inhibitors (disappearance of cytoplasmic microtubules and activation of movements of cell surface) are discussed.
D000818:Animals; D001567:Benzocycloheptenes / Q000494:pharmacology*; D003078:Colchicine / Q000494:pharmacology*; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis*; D004622:Embryo, Mammalian / Q000166:cytology; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008870:Microtubules / Q000187:drug effects; D013268:Stimulation, Chemical; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014747:Vinblastine / Q000494:pharmacology*
D001567:Benzocycloheptenes; D014316:Tritium; D014747:Vinblastine; D004247:DNA; D003078:Colchicine; D013936:Thymidine