Polyadenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA of HeLa cells: possible evidence for a precursor relationship.
Polyadenylate sequences have been found covalently linked in heterogeneous DNA-like nuclear RNA of HeLa cells. This poly(A) material seems homogeneous in size and accounts for about 0.5% of such RNA. Similar poly(A) sequences were found in rapidly-labeled polyribosomal RNA, thought to be messenger RNA. A possible model for mRNA synthesis from large heterogeneous nuclear RNA precursor molecules is discussed.
D000227:Adenine Nucleotides / Q000032:analysis* / Q000096:biosynthesis; D001345:Autoradiography; D001483:Base Sequence; D002499:Centrifugation, Density Gradient; D003593:Cytoplasm / Q000032:analysis; D003609:Dactinomycin / Q000494:pharmacology; D004589:Electrophoresis, Disc; D006367:HeLa Cells / Q000032:analysis*; D009691:Nucleic Acid Denaturation; D010759:Phosphorus Isotopes; D012313:RNA / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis; D012335:RNA, Ribosomal / Q000032:analysis*; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D000227:Adenine Nucleotides; D010759:Phosphorus Isotopes; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D014316:Tritium; D003609:Dactinomycin; D012313:RNA; D014529:Uridine