Mercurated dextran column chromatography for fractionating mononucleotides.
Mercurated dextran (polysaccharide) was found to fractionate mononucleotides according to their affinity for organomercurial Hg(+). The nucleotides are released from the chromatographic column in the sequence CMP, AMP, GMP, and TMP when borate buffer of increasing pH is used as an eluent.
D000249:Adenosine Monophosphate / Q000032:analysis; D001881:Borates; D002021:Buffers; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D003597:Cytosine Nucleotides / Q000032:analysis; D003911:Dextrans; D006150:Guanine Nucleotides / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007475:Ion Exchange Resins; D008628:Mercury; D009711:Nucleotides / Q000032:analysis*; D013053:Spectrophotometry; D013942:Thymine Nucleotides / Q000032:analysis
D001881:Borates; D002021:Buffers; D003597:Cytosine Nucleotides; D003911:Dextrans; D006150:Guanine Nucleotides; D007475:Ion Exchange Resins; D009711:Nucleotides; D013942:Thymine Nucleotides; D000249:Adenosine Monophosphate; D008628:Mercury