Analysis of enzyme variation in natural populations of the butterfly Colias eurytheme.
The use of internal standards in the separation of proteins by gel electrophoresis is shown to offer significant advantages over other control procedures heretofore used in electrophoretic surveys of enzyme variation in natural populations. By the use of such internal standards, a previously undetected class of micro-variability among the enzymes of wild insects has been demonstrated.
D000818:Animals; D002850:Chromatography, Gel; D004589:Electrophoresis, Disc; D014644:Genetic Variation; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D007313:Insecta / Q000378:metabolism*; D007527:Isoenzymes / Q000032:analysis*; D008291:Malate Dehydrogenase / Q000032:analysis; D008967:Molecular Biology; D010088:Oxidoreductases / Q000032:analysis; D011110:Polymorphism, Genetic; D013053:Spectrophotometry; D014969:Xanthine Oxidase
D007527:Isoenzymes; D010088:Oxidoreductases; D008291:Malate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D014969:Xanthine Oxidase