Investigation into rabbit infusion media for the growth of Mycoplasma gallisepticum antigens for inoculation into rabbits.
Serum cholesterol in rabbits was elevated, by intravenous inoculation of 20% Tween 80, to amounts thought to have been sufficient to support growth of Mycoplasma gallisepticum when 20% of such serum was added to rabbit infusion broth.However, better growth of the organism was obtained with a supplement of 5% rabbit serum and commercial cholesterol. Investigation showed that normal rabbit serum may be inhibitory, which would explain these findings. The commercial cholesterol was not antigenic.
D000818:Animals; D000941:Antigens; D000942:Antigens, Bacterial; D002417:Cattle; D002784:Cholesterol / Q000097:blood; D003470:Culture Media; D006168:Guinea Pigs; D006736:Horses; D007106:Immune Sera; D009174:Mycoplasma / Q000254:growth & development / Q000276:immunology*; D011506:Proteins / Q000032:analysis; D011817:Rabbits; D013501:Surface-Active Agents; D013552:Swine
D000941:Antigens; D000942:Antigens, Bacterial; D003470:Culture Media; D007106:Immune Sera; D011506:Proteins; D013501:Surface-Active Agents; D002784:Cholesterol