A novel closed-circular mitochondrial DNA with properties of a replicating intermediate.
A fraction of the covalently closed mitochondrial DNA in mouse L cells contains a replicated heavy-strand segment that is hydrogen bonded to the circular light strand. The inserted single strand is dissociable from the circular duplex at an elevated temperature.
D000818:Animals; D002247:Carbon Isotopes; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D006358:Hot Temperature; D007739:L Cells; D051379:Mice; D008854:Microscopy, Electron; D008928:Mitochondria / Q000032:analysis*; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D011487:Protein Conformation; D013936:Thymidine
D002247:Carbon Isotopes; D004247:DNA; D013936:Thymidine