Macromolecular inhibitor of fertilization in rabbit seminal plasma.
A large (86S) glycoprotein isolated from rabbit seminal plasma acts on sperm cells to block fertilization. The proportion of fertilized eggs recovered from does 1 day after tubal insemination of sperm 5-6 hr before or 2-3 hr after ovulation was significantly depressed when uterine-capacitated spermatozoa were exposed to this substance at concentrations of 20-72 mug of protein/ml. Rabbit seminal plasma was estimated to have about 2 mg/ml of this factor. Another large glycoprotein ( approximately 138S) is also present in rabbit seminal plasma; the function of this molecule awaits elucidation.
D000085:Acetates; D000818:Animals; D002482:Cellulose; D003864:Depression, Chemical; D004586:Electrophoresis; D005306:Fertilization / Q000187:drug effects*; D006023:Glycoproteins / Q000032:analysis / Q000494:pharmacology*; D046911:Macromolecular Substances / Q000032:analysis; D008297:Male; D011817:Rabbits; D012661:Semen / Q000032:analysis*; D013094:Spermatozoa / Q000187:drug effects; D013245:Sterilization, Reproductive; D014461:Ultracentrifugation; D014649:Vas Deferens / Q000601:surgery
D000085:Acetates; D006023:Glycoproteins; D046911:Macromolecular Substances; D002482:Cellulose