Compartmentalization of spermine and spermidine in the herpes simplex virion.
Enveloped particles of herpes simplex virus produced in human cells in culture contained spermidine and spermine, in a molar ratio of 1.6 +/- 0.2. The spermine present within the nucleocapsid is sufficient to neutralize at least 40% of the viral DNA. Disruption of the envelope with nonionic detergent and urea resulted in the selective loss of spermidine. Exogenous ornithine can function as a precursor to host and viral polyamines only before infection.
D002277:Carcinoma; D002460:Cell Line; D004279:DNA, Viral / Q000096:biosynthesis; D003902:Detergents / Q000494:pharmacology; D006561:Herpes Simplex / Q000378:metabolism; D006564:Herpesviridae / Q000032:analysis* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D009952:Ornithine / Q000378:metabolism; D011073:Polyamines / Q000032:analysis* / Q000096:biosynthesis; D011485:Protein Binding; D012867:Skin; D013095:Spermidine / Q000032:analysis / Q000378:metabolism; D013096:Spermine / Q000032:analysis* / Q000378:metabolism; D013997:Time Factors; D014316:Tritium; D014508:Urea / Q000494:pharmacology
D004279:DNA, Viral; D003902:Detergents; D011073:Polyamines; D014316:Tritium; D013096:Spermine; D014508:Urea; D009952:Ornithine; D013095:Spermidine