Human leukemic cells: in vitro growth of colonies containing the Philadelphia (Ph) chromosome.
Human leukemic cells with a marker (Philadelphia; Ph(1)) chromosome gave rise to granulocytic and mononuclear cell colonies when grown in vitro. All metaphases from a single colony were either Ph(1) positive or Ph(1) negative. No colonies contained a mixed cell population. This suggests that leukemic and normal cells exist simultaneously and that in vitro colonies are clonal in origin.
D001853:Bone Marrow / Q000254:growth & development*; D001854:Bone Marrow Cells; D002869:Chromosome Aberrations; D002904:Chromosomes, Human, 21-22 and Y; D002999:Clone Cells / Q000254:growth & development*; D006801:Humans; D007621:Karyotyping; D007951:Leukemia, Myeloid / Q000235:genetics / Q000473:pathology*; D007962:Leukocytes / Q000254:growth & development*; D008938:Mitosis