Expression of two X-linked genes in human hair follicles of double heterozygotes.
Expression of the two X-linked loci glucose-6-phosphate dehydrogenase (G6PD; EC 1.1.1.49) and hypoxanthine:guanine phosphoribosyltransferase (HGPRT; EC 2.4.2.8) was studied in single hair follicles of two females who were heterozygous for both of these genes (double heterozygotes). The coupling phase for these two loci was known to be g6pd A and hyprt(-) on the maternal X chromosome and g6pd B and hgprt(+) on the paternal X. Three phenotypic classes of hair follicles were observed in both double heterozygotes: G6pd A follicles with deficient HGPRT activity, G6pd B follicles with normal HGPRT activity, and G6pd AB follicles with intermediate HGPRT activity. These data directly demonstrate one of the predictions of the Lyon hypothesis that for two X-linked loci, those genes in cis position are turned on or off in a cell and its clone, while in trans only one gene or the other is expressed.
D000225:Adenine / Q000378:metabolism; D000227:Adenine Nucleotides; D000293:Adolescent; D000328:Adult; D002247:Carbon Isotopes; D002648:Child; D004586:Electrophoresis; D005260:Female; D005796:Genes; D005826:Genetics, Medical; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D006150:Guanine Nucleotides; D006197:Hair / Q000201:enzymology*; D006579:Heterozygote; D006801:Humans; D007042:Hypoxanthines; D007527:Isoenzymes / Q000032:analysis; D007926:Lesch-Nyhan Syndrome / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D009154:Mutation; D010428:Pentosephosphates; D011868:Radioisotopes; D012730:Sex Chromosomes; D014166:Transferases / Q000032:analysis; D014316:Tritium
D000227:Adenine Nucleotides; D002247:Carbon Isotopes; D006150:Guanine Nucleotides; D007042:Hypoxanthines; D007527:Isoenzymes; D010428:Pentosephosphates; D011868:Radioisotopes; D014316:Tritium; D005954:Glucosephosphate Dehydrogenase; D014166:Transferases; D000225:Adenine