Regulation of protein synthesis initiation in HeLa cells deprived of single essential amino acids.
In HeLa cells deprived of valine, histidine, or methionine initiation of protein synthesis decreases rapidly and disaggregation of polyribosomes also occurs. The mechanism of inhibition does not seem to involve the supply of RNA in the cell, and thus it differs from the initiation of inhibition at elevated temperatures. Polyribosomes rapidly form again if the missing amino acid is restored, even in the presence of actinomycin D.
D000596:Amino Acids / Q000378:metabolism*; D001345:Autoradiography; D002247:Carbon Isotopes; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D003470:Culture Media; D003609:Dactinomycin / Q000494:pharmacology; D006367:HeLa Cells / Q000378:metabolism*; D006639:Histidine / Q000378:metabolism; D006358:Hot Temperature; D006801:Humans; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D010441:Peptide Chain Elongation, Translational; D010442:Peptide Chain Initiation, Translational; D014176:Protein Biosynthesis; D012335:RNA, Ribosomal / Q000096:biosynthesis; D012270:Ribosomes / Q000378:metabolism*; D013997:Time Factors; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D014633:Valine / Q000378:metabolism
D000596:Amino Acids; D002247:Carbon Isotopes; D003470:Culture Media; D012335:RNA, Ribosomal; D014316:Tritium; D003609:Dactinomycin; D006639:Histidine; D008715:Methionine; D007930:Leucine; D014633:Valine; D014529:Uridine