Mechanism of polymerization of the Salmonella O-antigen: utilization of lipid-linked intermediates.
Cell envelope fractions from Salmonella can utilize exogenous lipid-linked intermediates for the synthesis of polymeric O-antigen. We describe a method for preparing aqueous suspensions of lipid intermediates and show that freezing and thawing of cell envelope-lipid intermediate mixtures is required for efficient synthesis. The lipid intermediates move freely in the hydrophobic environment of the membrane.
D000941:Antigens / Q000096:biosynthesis*; D000942:Antigens, Bacterial / Q000096:biosynthesis; D002247:Carbon Isotopes; D002462:Cell Membrane / Q000201:enzymology* / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D005615:Freezing; D005690:Galactose; D005964:Glucosyltransferases / Q000378:metabolism; D006602:Hexosyltransferases / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism*; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism; D009713:Nucleotidyltransferases / Q000378:metabolism; D012475:Salmonella / Q000201:enzymology* / Q000276:immunology / Q000378:metabolism; D014166:Transferases / Q000378:metabolism
D000941:Antigens; D000942:Antigens, Bacterial; D002247:Carbon Isotopes; D008070:Lipopolysaccharides; D009702:Nucleoside Diphosphate Sugars; D014166:Transferases; D005964:Glucosyltransferases; D006602:Hexosyltransferases; D009713:Nucleotidyltransferases; D005690:Galactose