Isolation of an organ-specific leucyl-tRNA synthetase from soybean seedling.
The leucyl-tRNA synthetase activity from cotyledons of 4-day-old soybean seedlings has been fractionated into three components. One of these exclusively acylates two of the six tRNA(Leu) species present in this tissue. The remaining two enzyme fractions charge the other four tRNA(Leu) species equally well. Soybean hypocotyls appear to contain only the last two enzyme fractions.
D002845:Chromatography; D006846:Hydrocarbons, Halogenated; D006882:Hydroxyapatites; D007930:Leucine / Q000378:metabolism; D008025:Ligases / Q000032:analysis / Q000302:isolation & purification*; D009928:Organ Specificity; D012343:RNA, Transfer / Q000378:metabolism; D012639:Seeds / Q000201:enzymology; D013025:Glycine max
D006846:Hydrocarbons, Halogenated; D006882:Hydroxyapatites; D012343:RNA, Transfer; D008025:Ligases; D007930:Leucine