Interaction of eukaryote initiator methionyl-tRNA with the eukaryote equivalent of bacterial elongation factor T and guanosine triphosphate.
The initiator tRNA, methionyl-tRNA(i) (Met), of yeast and wheat germ forms relatively unstable ternary complexes with their corresponding elongation factors T and GTP. Such complexes can be demonstrated only with fast separation techniques such as Sephadex G-50 and Millipore filtration, but not with the slow Sephadex G-100 method, although both techniques yield stable ternary complexes with all other aminoacyl-tRNAs, including the internal Met-tRNA(m) (Met). To bind yeast-initiating Met-tRNA(i) (Met) to ribosomes, initiation factors present in a ribosomal wash fraction from yeast are needed.
D001665:Binding Sites; D002247:Carbon Isotopes; D002467:Cell Nucleus; D002482:Cellulose; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005374:Filtration; D006160:Guanosine Triphosphate / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D008851:Micropore Filters; D010452:Peptide Biosynthesis; D010441:Peptide Chain Elongation, Translational; D010442:Peptide Chain Initiation, Translational; D010649:Phenylalanine / Q000378:metabolism; D011485:Protein Binding; D012343:RNA, Transfer / Q000302:isolation & purification / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D013997:Time Factors; D014908:Triticum / Q000378:metabolism; D014316:Tritium; D015003:Yeasts / Q000378:metabolism
D002247:Carbon Isotopes; D014316:Tritium; D010649:Phenylalanine; D006160:Guanosine Triphosphate; D002482:Cellulose; D012343:RNA, Transfer; D008715:Methionine