Release, identification, and isolation of messenger RNA from mammalian ribosomes.
The puromycin-induced dissociation, at high ionic strength, of active ribosomes from reticulocytes resulted in the release of protein-free, apparently undegraded, messenger RNA for globin, which was identified by centrifugation on a sucrose density gradient. This procedure should make messenger RNA from various cells available for isolation on a large scale, and has several advantages over procedures that use detergents or magnesium chelators.
D000818:Animals; D002499:Centrifugation, Density Gradient; D008722:Methods; D011691:Puromycin / Q000494:pharmacology; D012333:RNA, Messenger / Q000302:isolation & purification*; D012335:RNA, Ribosomal / Q000302:isolation & purification; D011817:Rabbits; D012156:Reticulocytes / Q000187:drug effects; D012270:Ribosomes / Q000187:drug effects
D012333:RNA, Messenger; D012335:RNA, Ribosomal; D011691:Puromycin