Bovine liver glutamate dehydrogenase: tentative amino acid sequence; identification of a reactive lysine; nitration of a specific tyrosine and loss of allosteric inhibition by guanosine triphosphate.
A tentative but almost complete amino acid sequence for the subunit peptide chain of bovine liver glutamate dehydrogenase indicates a minimal size of 506 residues with a molecular weight of 56,100, in accord with the physical size of the subunit of 55,900. Inactivation with pyridoxal 5'-phosphate, followed by reduction with sodium borohydride, has permitted identification of the essential lysine as residue 97. Nitration of tyrosine-412 is accompanied by loss of the allosteric inhibitory effect of guanosine triphosphate. Comparison of the sequences of glutamate dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase has indicated that only two 12-residue sequences are similar in the two enzymes; this sequence includes reactive lysine-97 of the former enzyme.
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D005969:Glutamate Dehydrogenase / Q000032:analysis*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000032:analysis; D006160:Guanosine Triphosphate / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology*; D008239:Lysine / Q000032:analysis*; D008722:Methods; D008970:Molecular Weight; D009566:Nitrates; D013237:Stereoisomerism; D014443:Tyrosine
D009566:Nitrates; D014443:Tyrosine; D006160:Guanosine Triphosphate; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D005969:Glutamate Dehydrogenase; D008239:Lysine