DNA polymerase activities and nucleic acid components of virions isolated from a spontaneous mammary carcinoma from a rhesus monkey.
Virions isolated from a spontaneous mammary carcinoma of a rhesus monkey and propagated in human cells possess an RNA-instructed DNA polymerase. They also exhibit DNA polymerase activities that respond to either double-stranded DNA or synthetic RNA.DNA hybrid complexes as templates. The virion has been shown to have a density of 1.16 g/ml and to contain a nucleic acid species of high molecular weight (sedimentation coefficient, 60-70 S), which bands as RNA at 1.670 in a Cs(2)SO(4) equilibrium density gradient. In addition, the virions contain species of low molecular weight (4-6 S) that consist of RNA as well as components banding at densities characteristic of DNA.RNA complexes. The nucleoid of this virion has been isolated and shown to have a density of 1.23 g/ml; it also contains a 60-70S nucleic acid species.
D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D046508:Culture Techniques; D003597:Cytosine Nucleotides / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis / Q000494:pharmacology; D004254:DNA Nucleotidyltransferases / Q000032:analysis*; D004279:DNA, Viral; D003854:Deoxyribonucleotides / Q000494:pharmacology; D005347:Fibroblasts; D006801:Humans; D007962:Leukocytes; D008251:Macaca; D008325:Mammary Neoplasms, Experimental; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000032:analysis*; D009858:Oncogenic Viruses / Q000201:enzymology; D011119:Polynucleotides / Q000494:pharmacology; D012328:RNA Viruses / Q000032:analysis / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D012367:RNA, Viral / Q000032:analysis* / Q000494:pharmacology; D012260:Ribonucleases / Q000494:pharmacology; D013698:Templates, Genetic; D013942:Thymine Nucleotides / Q000378:metabolism; D014316:Tritium
D003597:Cytosine Nucleotides; D004279:DNA, Viral; D003854:Deoxyribonucleotides; D009696:Nucleic Acids; D011119:Polynucleotides; D012367:RNA, Viral; D013942:Thymine Nucleotides; D014316:Tritium; D004247:DNA; D004254:DNA Nucleotidyltransferases; D012260:Ribonucleases