Translocation of messenger RNA and "accommodation" of fMet-tRNA.
Messenger RNA is moved a distance of approximately three nucleotides in the 5' direction relative to the ribosome during the translocation of peptidyl-tRNA from the A to the P site. This movement is catalyzed by G factor and is dependent on the hydrolysis of GTP. In contrast, mRNA is not moved during the f(2)-catalyzed hydrolysis of GTP that is involved in the activation of ribosome-bound fMet-tRNA. This second type of GTP-dependent reaction has been named "Accommodation".
D002247:Carbon Isotopes; D002854:Chromatography, Paper; D005561:Formates; D005815:Genetic Code; D006160:Guanosine Triphosphate / Q000378:metabolism; D006868:Hydrolysis; D008715:Methionine; D008956:Models, Chemical; D008961:Models, Structural; D009711:Nucleotides / Q000032:analysis; D010452:Peptide Biosynthesis; D010441:Peptide Chain Elongation, Translational; D010442:Peptide Chain Initiation, Translational; D011691:Puromycin / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism; D014316:Tritium
D002247:Carbon Isotopes; D005561:Formates; D009711:Nucleotides; D012333:RNA, Messenger; D014316:Tritium; D011691:Puromycin; D006160:Guanosine Triphosphate; D012343:RNA, Transfer; D008715:Methionine