Cellular localization of anti-DNP-PLL and anticonveyor albumin antibodies in genetic nonresponder guinea pigs immunized with DNP-PLL albumin complexes.
Genetic nonresponder guinea pigs incapable of an immune response to DNP-PLL alone were immunized with DNP-PLL complexed to ovalbumin or bovine serum albumin. Under these circumstances the animals produce both anti-DNP-PLL antibodies and antibodies directed against the conveyor albumin. Thus immune response to DNP-PLL complexed to conveyor albumin molecules can serve as a simple model of hapten-carrier relationships. To explore these relationships the question whether these two types of antibody are synthesized by the same or by different plasma cells was investigated by a combination of a double immunofluorescent technique and radioautographic localization of radioactive antigen. It was shown that the anti-DNP-PLL antibodies and the antibodies against the carrier albumin molecule were produced in separate cells. No cell-producing antibodies with both specificities were detected out of 526 cells studied.
D000818:Animals; D000917:Antibody Formation; D001345:Autoradiography; D004140:Dinitrophenols / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D006241:Haptens; D008239:Lysine / Q000494:pharmacology; D010047:Ovalbumin / Q000494:pharmacology; D010950:Plasma Cells / Q000378:metabolism*; D011108:Polymers
D004140:Dinitrophenols; D006241:Haptens; D011108:Polymers; D010047:Ovalbumin; D008239:Lysine