Selection of sucrose-dependent Escherichia coli to obtain envelope mutants and fragile cultures.
Mutants isolated as sucrose-dependent include many with apparent envelope defects, which fact often leads to filamentous growth or lysis in the absence of sucrose. One mutant can grow exponentially in 4 percent sucrose, but is very fragile: it releases all of its RNA and soluble protein when treated with 0.5 percent sodium deoxycholate. These characteristics permit the study of unstable structures and rapid processes in actively growing cells.
D001647:Bile Acids and Salts / Q000494:pharmacology*; D004926:Escherichia coli / Q000254:growth & development*; D009154:Mutation; D012313:RNA / Q000378:metabolism*; D013395:Sucrose; D014885:Waxes / Q000494:pharmacology
D001647:Bile Acids and Salts; D014885:Waxes; D013395:Sucrose; D012313:RNA