Aging of Escherichia coli.
Clifton, C. E. (Stanford University, Stanford, Calif.). Aging of Escherichia coli. J. Bacteriol. 92:905-912. 1966.-The rates of endogenous and exogenous (glucose) respiration decreased much more rapidly than did the viable count during the first 24 hr of aging of washed, C(14)-labeled cells of Escherichia coli K-12 suspended in a basal salt medium devoid of ammonium salts. The rates of decrease of respiration and of death approached each other as the age of the cells increased, but death was not the only factor involved in decreased respiratory activity of the suspensions. The greatest decrease in cellular contents with aging was noted in the ribonucleic acid fraction, of which the ribose appeared to be oxidized, while uracil accumulated in the suspension medium. The viable count and respiratory activities remained higher in glucose-fed than in nonfed suspensions. Proline-labeled cells fed glucose tended to lose more of their proline and to convert more proline into C(14)O(2) than in unfed controls. On the other hand, uracil-labeled cells fed glucose retained more of the uracil than did nonfed cells, but glucose elicited some oxidation of uracil. An exogenous energy source such as glucose aided in the maintenance of a population, but it was not the only factor needed for such maintenance.
D002247:Carbon Isotopes; D003124:Colorimetry; D004926:Escherichia coli / Q000254:growth & development* / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D011392:Proline / Q000494:pharmacology; D012329:RNA, Bacterial / Q000378:metabolism; D012266:Ribose / Q000494:pharmacology; D014498:Uracil / Q000494:pharmacology
D002247:Carbon Isotopes; D012329:RNA, Bacterial; D014498:Uracil; D012266:Ribose; D011392:Proline; D005947:Glucose