Inhibition of growth of leukemia cells by enzymic folate depletion.
A new bacterial enzyme, designated as carboxypeptidase G(1), inhibited growth of the L5178Y and L1210 murine leukemias, as well as the Walker carcinoma and the human lymphoblastoid line (RPMI 4265), propogated in vitro. This enzyme hydrolyzes the glutamate moiety from both oxidized and reduced folate forms, and thus it may prove to be of value in creating rapid folate depletion in vivo.
D000818:Animals; D002268:Carboxypeptidases / Q000494:pharmacology*; D002279:Carcinoma 256, Walker / Q000378:metabolism; D002460:Cell Line / Q000378:metabolism; D003470:Culture Media; D003864:Depression, Chemical; D005492:Folic Acid / Q000378:metabolism*; D006801:Humans; D007042:Hypoxanthines / Q000494:pharmacology; D066298:In Vitro Techniques; D002955:Leucovorin / Q000378:metabolism; D007939:Leukemia L1210 / Q000378:metabolism*; D007942:Leukemia, Experimental / Q000378:metabolism*; D008727:Methotrexate / Q000494:pharmacology; D011549:Pseudomonas / Q000201:enzymology; D012694:Serine / Q000494:pharmacology; D013936:Thymidine / Q000494:pharmacology
D003470:Culture Media; D007042:Hypoxanthines; D012694:Serine; D005492:Folic Acid; D002268:Carboxypeptidases; D002955:Leucovorin; D013936:Thymidine; D008727:Methotrexate