Normal serum cytotoxicity for P32-labeled smooth Enterobacteriaceae. II. Fate of macromolecular and lipid phosphorus of damaged cells.
Spitznagel, John K. (University of North Carolina, Chapel Hill). Normal serum cytotoxicity for P(32)-labeled smooth Enterobacteriaceae. II. Fate of macromolecular and lipid phosphorus of damaged cells. J. Bacteriol. 91:148-152. 1966.-Fresh, normal adult guinea pig serum released micro- and macromolecular forms of metabolically incorporated P(32) from smooth Escherichia coli. A significant amount of P(32)-labeled phospholipid was also released. The overall acid solubility distribution of the P(32) label in bacterial suspensions was very little affected in fresh serum, although P(32)-labeled cytoplasmic contents and cell walls of E. coli lost some P(32) when they were suspended in fresh or heat-inactivated normal serum. These findings confirmed and extended earlier evidence that the peripheral wall and membrane structures of smooth E. coli are extensively damaged by factors in fresh normal serum.
D001769:Blood; D002462:Cell Membrane / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D046911:Macromolecular Substances; D010743:Phospholipids / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism*; D010759:Phosphorus Isotopes; D012329:RNA, Bacterial / Q000378:metabolism
D046911:Macromolecular Substances; D010743:Phospholipids; D010759:Phosphorus Isotopes; D012329:RNA, Bacterial; D010758:Phosphorus