Studies on insulin biosynthesis. Subcellular distribution of leucine-H3 radioactivity during incubation of goosefish islet tissue.
Islet tissue from the goosefish (Lophius piscatorius) was incubated in the presence of leucine-H(3). The tissue was then separated into subcellular fractions and the radioactivity determined in total acid alcohol-soluble proteins, insoluble proteins, and insulin. At any time, microsomal protein had a higher activity than secretion granule protein. Pulse-chase experiments further suggest the microsomes as primary sites of protein synthesis. The data are evidence for microsomal synthesis of insulin and for its subsequent transfer into the secretion granules.
D000818:Animals; D004586:Electrophoresis; D005399:Fishes; D066298:In Vitro Techniques; D007328:Insulin / Q000096:biosynthesis*; D007515:Islets of Langerhans / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism; D009113:Muramidase / Q000494:pharmacology; D014176:Protein Biosynthesis; D011874:Radiometry; D014316:Tritium
D007328:Insulin; D014316:Tritium; D009113:Muramidase; D007930:Leucine