Glucose and gluconate metabolism in an Escherichia coli mutant lacking phosphoglucose isomerase.
A single gene mutant lacking phosphoglucose isomerase (pgi) was selected after ethyl methane sulfonate mutagenesis of Escherichia coli strain K-10. Enzyme assays revealed no pgi activity in the mutant, whereas levels of glucokinase, glucose-6-phosphate dehydrogenase, and gluconate-6-phosphate dehydrogenase were similar in parent and mutant. The amount of glucose released by acid hydrolysis of the mutant cells after growth on gluconate was less than 2% that released from parent cells; when grown in the presence of glucose, mutant and parent cells contained the same amount of glucose residues. The mutant grew on glucose one-third as fast as the parent; it also grew much slower than the parent on galactose, maltose, and lactose. On fructose, gluconate, and other carbon sources, growth was almost normal. In both parent and mutant, gluconokinase and gluconate-6-phosphate dehydrase were present during growth on gluconate but not during growth on glucose. Assay and degradation of alanine from protein hydrolysates after growth on glucose-1-(14)C and gluconate-1-(14)C showed that in the parent strain glucose was metabolized by the glycolytic path and the hexose monophosphate shunt. Gluconate was metabolized by the Entner-Doudoroff path and the hexose monophosphate shunt. The mutant used glucose chiefly by the shunt, but may also have used the Entner-Doudoroff path to a limited extent.
D000409:Alanine / Q000378:metabolism; D002247:Carbon Isotopes; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism*; D005941:Glucokinase / Q000378:metabolism; D005942:Gluconates / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D008967:Molecular Biology; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation; D010088:Oxidoreductases / Q000378:metabolism
D002247:Carbon Isotopes; D005942:Gluconates; D009153:Mutagens; D010088:Oxidoreductases; D005954:Glucosephosphate Dehydrogenase; D005941:Glucokinase; D005956:Glucose-6-Phosphate Isomerase; D005947:Glucose; D000409:Alanine