Mechanism of inactivation of transforming deoxyribonucleic acid by X rays.
Transforming deoxyribonucleic acid (DNA) from Haemophilus influenzae was exposed to X rays either in phosphate buffer or in 10% yeast extract. Relations between determinations of biological inactivation, DNA uptake by competent H. influenzae, integration of DNA into the competent cell genome, and induced single-and double-strand breaks indicate that transforming DNA is inactivated by the direct and the indirect effect of X radiation primarily because integration of DNA is prevented as a result of the production of double-strand breaks.
D002021:Buffers; D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D002876:Chromosomes, Bacterial; D003470:Culture Media; D004261:DNA Replication; D004269:DNA, Bacterial / Q000378:metabolism / Q000528:radiation effects*; D006193:Haemophilus influenzae / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D008970:Molecular Weight; D010710:Phosphates; D011830:Radiation Effects; D012440:Saccharomyces; D013395:Sucrose; D013936:Thymidine / Q000378:metabolism; D014170:Transformation, Genetic; D014316:Tritium
D002021:Buffers; D002247:Carbon Isotopes; D003470:Culture Media; D004269:DNA, Bacterial; D010710:Phosphates; D014316:Tritium; D013395:Sucrose; D013936:Thymidine