Measurement of binding of chloramphenicol by intact cells.
The binding of chloramphenicol to intracellular components of intact cells was measured by procedures based on a silicone-wash technique. The number of stereospecifically bound molecules of chloramphenicol increased with external concentration to a saturation value equal to the number of ribosomes per cell. Chloramphenicol is therefore believed to be attached stereospecifically by a weak bond, most probably to a single site on the 50S ribosome. This bond was found to be temperature-dependent and appeared to be responsible for inhibition of protein synthesis.
D001426:Bacterial Proteins / Q000096:biosynthesis; D002247:Carbon Isotopes; D002701:Chloramphenicol / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D013696:Temperature; D014316:Tritium
D001426:Bacterial Proteins; D002247:Carbon Isotopes; D014316:Tritium; D002701:Chloramphenicol