Polysomes extracted from Escherichia coli by freeze-thaw-lysozyme lysis.
Polysomes can be extracted from Escherichia coli by freezing and thawing in the presence of lysozyme, followed by treatment with sodium deoxycholate. The method is simple and convenient; the yields consistently high.
D001647:Bile Acids and Salts; D004926:Escherichia coli; D005615:Freezing; D009113:Muramidase; D012270:Ribosomes; D014461:Ultracentrifugation
D001647:Bile Acids and Salts; D009113:Muramidase