A simple freeze-substitution method for electron microscopy.
D002467:Cell Nucleus; D003593:Cytoplasm; D004330:Drosophila / Q000166:cytology; D005615:Freezing; D006652:Histological Techniques; D008854:Microscopy, Electron; D008928:Mitochondria; D009491:Neurospora / Q000166:cytology; D012270:Ribosomes; D012469:Salivary Glands / Q000166:cytology