Synchronously dividing bacterial cultures. I. Synchrony following depletion and resupplementation of a required amino acid in Escerichia coli.
Matney, Thomas S. (The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston, Texas), and Joan C. Suit. Synchronously dividing bacterial cultures. I. Synchrony following depletion and resupplementation of a required amino acid in Escherichia coli. J. Bacteriol. 92:960-966. 1966.-A procedure was developed for phasing large-volume cultures of Escherichia coli K-12 with regard to cell division. The method consists of permitting the bacteria to exhaust a growth-limiting supply of a required amino acid, starving the culture, resupplementing with an excess of the amino acid, and following the ensuing growth by usual counting procedures.
D000596:Amino Acids / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D004926:Escherichia coli / Q000254:growth & development* / Q000378:metabolism*; D012329:RNA, Bacterial / Q000378:metabolism; D013053:Spectrophotometry
D000596:Amino Acids; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012329:RNA, Bacterial