Chemical and biological properties of an extracellular lipopolysaccharide from Escherichia coli grown under lysine-limiting conditions.
Lipopolysaccharide was prepared from the extracellular lipoglycopeptide produced by the lysine-requiring mutant Escherichia coli A.T.C.C. 12408 grown under lysine-limiting conditions. The lipid moiety, containing glucosamine phosphate and four fatty acids (lauric acid, myristic acid, beta-hydroxymyristic acid and palmitic acid) corresponded in composition to lipid A of known bacterial lipopolysaccharides. The components of the polysaccharide moiety were d-glucose, d-galactose, l-glycero-d-manno-heptose, 3-deoxy-2-oxo-octonic acid, ethanolamine and phosphate. These are the constituents of the polysaccharide of the cell-wall antigens from rough strains of E. coli. Lipopolysaccharides were also prepared from whole cells of E. coli 12408 grown with excess or limited amounts of lysine; they were identical in carbohydrate composition with the extracellular lipopolysaccharide. The biological properties of this material also resembled those of known lipopolysaccharides; it was antigenic, pyrogenic, toxic and had adjuvant activity.
D000818:Animals; D000941:Antigens / Q000032:analysis; D001681:Biological Assay; D002849:Chromatography, Gas; D002854:Chromatography, Paper; D004586:Electrophoresis; D004926:Escherichia coli / Q000378:metabolism*; D006386:Hemagglutination Tests; D008070:Lipopolysaccharides / Q000032:analysis*; D008239:Lysine / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D011749:Pyrogens / Q000032:analysis; D011817:Rabbits
D000941:Antigens; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D011749:Pyrogens; D010088:Oxidoreductases; D008239:Lysine