Formation of lactyl-coenzyme A and pyruvyl-coenzyme A from lactic acid by Escherichia coli.
Megraw, Robert E. (Albert Einstein Medical Center, Philadelphia, Pa.), Henry C. Reeves, and Samuel J. Ajl. Formation of lactyl-coenzyme A and pyruvyl-coenzyme A from lactic acid by Escherichia coli. J. Bacteriol. 90:984-988. 1965.-Cell extracts of propionate-adapted Escherichia coli were found to contain a lactyl-coenzyme A (CoA) synthetase which catalyzes the formation of the CoA thiolester from lactate, CoA, and cofactors. The extracts also catalyzed the nicotinamide adenine dinucleotide-dependent oxidation of lactyl-CoA. The product of this oxidation was identified chromatographically as pyruvyl-CoA.
D002845:Chromatography; D003065:Coenzyme A / Q000096:biosynthesis*; D004926:Escherichia coli / Q000201:enzymology*; D066298:In Vitro Techniques; D007773:Lactates / Q000378:metabolism*; D008025:Ligases / Q000096:biosynthesis*; D011773:Pyruvates / Q000378:metabolism*
D007773:Lactates; D011773:Pyruvates; D008025:Ligases; D003065:Coenzyme A