Accumulation of sulfite by a sulfate-using revertant of Salmonella pullorum.
Sulfate-utilizing revertants of a cysteine auxotroph of Salmonella pullorum have been found that cause an accumulation in growth medium of a sulfur compound more oxidized than sulfide. The sulfur compound was presumptively identified as sulfite by the formation of a colored complex in the presence of basic fuchsin and formaldehyde, oxidation to sulfate by peroxide, and acid-volatility. The acid-volatile material was identified as sulfite by formation of an S-sulfonyl derivative of 5,5'-dithio-bis(2-nitrobenzoic acid) which was chromatographically and electrophoretically identical to an authentic S-sulfonyl derivative. The presence of sulfate in minimal medium is required for sulfite accumulation, and both cysteine and selenate inhibit the accumulation. No evidence was obtained to indicate that a reduced sulfur compound was the precursor of the accumulated sulfite.
D002845:Chromatography; D003470:Culture Media; D003545:Cysteine / Q000378:metabolism / Q000494:pharmacology; D004586:Electrophoresis; D005827:Genetics, Microbial; D008967:Molecular Biology; D009154:Mutation; D010084:Oxidation-Reduction; D012475:Salmonella / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism*; D013447:Sulfites / Q000032:analysis / Q000378:metabolism*
D003470:Culture Media; D013431:Sulfates; D013447:Sulfites; D003545:Cysteine