Separation of N-acetyl-alpha-glucosaminidase and N-acetyl-alpha-galactosaminidase from ox spleen. Cleavage of the O-glycosidic linkage between carbohydrate and polypeptide in ovine and bovine submaxillary glycoprotein by N-acetyl-alpha-galactosaminidase.
D000818:Animals; D001555:Benzene Derivatives; D002241:Carbohydrates; D002417:Cattle; D002474:Cell-Free System; D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002621:Chemistry; D002850:Chromatography, Gel; D004789:Enzyme Activation; D005690:Galactose; D005944:Glucosamine; D006023:Glycoproteins; D006026:Glycoside Hydrolases / Q000302:isolation & purification / Q000378:metabolism; D006595:Hexosamines; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008722:Methods; D010455:Peptides; D000644:Quaternary Ammonium Compounds; D012756:Sheep; D013154:Spleen / Q000201:enzymology*; D013363:Submandibular Gland; D013696:Temperature; D013816:Thermodynamics; D013997:Time Factors; D014500:Uracil Nucleotides
D001555:Benzene Derivatives; D002241:Carbohydrates; D006023:Glycoproteins; D006595:Hexosamines; D010455:Peptides; D000644:Quaternary Ammonium Compounds; D014500:Uracil Nucleotides; D006026:Glycoside Hydrolases; D005944:Glucosamine; D005690:Galactose