The eighth component of human complement (C8): isolation, characterization, and hemolytic efficiency.
A method was described allowing the isolation of the eighth component of complement, C8, from human serum. C8 is an immunochemically definable, heretofore unrecognized serum protein with a molecular weight of approximately 150,000 and an electrophoretic mobility of a gamma(1)-globulin. It may be obtained with 5% yield as a 5000-fold purified, physicochemically and immunochemically homogeneous protein. Under optimal experimental conditions, a few molecules of C8 were found sufficient for lysis of a cell.
D000818:Animals; D001797:Blood Protein Electrophoresis; D002845:Chromatography; D002850:Chromatography, Gel; D003165:Complement System Proteins / Q000302:isolation & purification*; D006461:Hemolysis; D006801:Humans; D007120:Immunochemistry; D007122:Immunoelectrophoresis; D008722:Methods; D011817:Rabbits; D014461:Ultracentrifugation
D003165:Complement System Proteins