Protein synthesis in sonically damaged Escherichia coli.
By gentle sonic treatment, Escherichia coli cells were modified to permit penetration of actinomycin D, adenosine triphosphate, trypsin, ribonuclease, and polyuridylic acid. The behavior of these "soniplasts" as protein-synthesizing particles was investigated.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000596:Amino Acids / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis*; D002247:Carbon Isotopes; D002463:Cell Membrane Permeability; D003609:Dactinomycin / Q000494:pharmacology; D004926:Escherichia coli / Q000032:analysis / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D008623:Mercaptoethanol / Q000032:analysis; D008854:Microscopy, Electron; D011691:Puromycin / Q000494:pharmacology; D012329:RNA, Bacterial / Q000032:analysis; D012260:Ribonucleases / Q000494:pharmacology; D013386:Succinates / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology; D014500:Uracil Nucleotides / Q000494:pharmacology; D014732:Vibration
D000596:Amino Acids; D001426:Bacterial Proteins; D002247:Carbon Isotopes; D012329:RNA, Bacterial; D013386:Succinates; D014500:Uracil Nucleotides; D003609:Dactinomycin; D011691:Puromycin; D008623:Mercaptoethanol; D000255:Adenosine Triphosphate; D012260:Ribonucleases; D014357:Trypsin; D005947:Glucose