Synthesis of vaccinia viral proteins in cytoplasmic extracts. II. Identification of early and late viral proteins.
The synthesis of vaccinia viral proteins has been studied in a cell-free system prepared from vaccinia virus-infected HeLa cells. The radioactively labeled proteins were identified as viral proteins by immunodiffusion, disc gel electrophoresis, and disc gel-immunoelectrophoresis. The cytoplasmic extracts, obtained from infected cells at different times during viral replication, synthesized the corresponding "early" or "late" viral proteins.
D001345:Autoradiography; D002247:Carbon Isotopes; D002474:Cell-Free System; D003593:Cytoplasm; D004589:Electrophoresis, Disc; D006367:HeLa Cells; D007122:Immunoelectrophoresis; D012333:RNA, Messenger; D012367:RNA, Viral; D013997:Time Factors; D014316:Tritium; D014616:Vaccinia virus / Q000254:growth & development*; D014764:Viral Proteins / Q000032:analysis* / Q000096:biosynthesis*
D002247:Carbon Isotopes; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium