Effect of cholesteryl 14-methylhexadecanoate on the activity of some amino acid-transfer ribonucleic acid ligases from mammalian tissues.
1. l-Tyrosine-, l-alanine-, l-tryptophan- and l-threonine-tRNA ligases (where tRNA is transfer RNA) were purified from mammalian tissues and the relative contents of cholesteryl 14-methylhexadecanoate were determined in fractions obtained during the isolation. Purified enzymes were extracted with various organic solvents. 2. Cholesteryl 14-methylhexadecanoate contents in purified ligases were up to 210-fold that in the starting material. Different enzymes showed different contents of this cholesteryl ester. 3. Extracted enzymes lost in most cases their ability to catalyse formation of the aminoacylhydroxamate and aminoacyl-tRNA complexes. Enzymes extracted with various solvents showed a variable decreased activity. 4. The original activity could be restored to 70-100% by the addition of cholesteryl 14-methylhexadecanoate. Cholesteryl palmitate, cholesteryl margarate and cholesteryl stearate were inactive in this respect. 5. Incubation mixtures of extracted enzymes with cholesteryl 14-methylhexadecanoate added showed an initial delay in the time-course of both reactions assayed. 6. It is concluded that the effect of cholesteryl 14-methylhexadecanoate on the activity of amino acid-tRNA ligases seems to be specific and that this compound may play some role in the function of these enzymes.
D000409:Alanine / Q000032:analysis; D000818:Animals; D002247:Carbon Isotopes; D008025:Ligases / Q000032:analysis*; D008099:Liver / Q000201:enzymology; D010179:Pancreas / Q000201:enzymology; D012343:RNA, Transfer; D051381:Rats; D013261:Sterols / Q000032:analysis*; D013912:Threonine / Q000032:analysis; D014316:Tritium; D014364:Tryptophan / Q000032:analysis; D014443:Tyrosine / Q000032:analysis
D002247:Carbon Isotopes; D013261:Sterols; D014316:Tritium; D013912:Threonine; D014443:Tyrosine; D014364:Tryptophan; D012343:RNA, Transfer; D008025:Ligases; D000409:Alanine