The metabolism of [14C] nicotine in the cat.
The metabolism of [2'-(14)C]nicotine given as an intravenous injection in small doses to anaesthetized and unanaesthetized cats has been studied. A method is described for the quantitative determination of [(14)C]nicotine and [(14)C]cotinine in tissues and body fluids. Nanogram amounts of these compounds have been detected. After a single dose of 40mug. of [(14)C]nicotine/kg., 55% of the injected radioactivity was excreted in the urine within 24hr., but only 1% of this radioactivity was unchanged nicotine. [(14)C]Nicotine is metabolized extremely rapidly, [(14)C]cotinine appearing in the blood within 2.5min. of intravenous injection. [(14)C]Nicotine accumulates rapidly in the brain and 15min. after injection 90% of the radioactivity still represents [(14)C]nicotine. Metabolites of [(14)C]nicotine have been identified in liver and urine extracts. [(14)C]Nicotine-1'-oxide has been detected in both liver and urine.
D000818:Animals; D001345:Autoradiography; D001646:Bile / Q000032:analysis; D001923:Brain Chemistry; D002247:Carbon Isotopes; D002415:Cats; D002855:Chromatography, Thin Layer; D005260:Female; D005750:Gastric Juice / Q000032:analysis; D007275:Injections, Intravenous; D008099:Liver / Q000032:analysis; D008297:Male; D008722:Methods; D009538:Nicotine / Q000032:analysis / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism*; D011760:Pyrrolidinones / Q000032:analysis / Q000097:blood
D002247:Carbon Isotopes; D011760:Pyrrolidinones; D009538:Nicotine