Studies on the conversion of 60s yeast ribosomes into a 50S component.
The process of conversion of the larger (60s) subunit of yeast ribosomes into a 50s component was studied. The release of any RNA or protein material during conversion was assayed by using (32)P- or (35)S-labelled ribosomes; ribosomal RNA distributions of the particles were examined and protein/RNA ratios of subunits were determined. The change in sedimentation coefficients was found to be due, not to loss of material, but to a structural change. The change in structure was shown by electron microscopy.
D008274:Magnesium; D008854:Microscopy, Electron; D010759:Phosphorus Isotopes; D011506:Proteins / Q000032:analysis; D012313:RNA / Q000032:analysis; D012270:Ribosomes / Q000032:analysis / Q000378:metabolism*; D012440:Saccharomyces / Q000166:cytology / Q000378:metabolism*; D013460:Sulfur Isotopes; D014461:Ultracentrifugation
D010759:Phosphorus Isotopes; D011506:Proteins; D013460:Sulfur Isotopes; D012313:RNA; D008274:Magnesium