The de-repression of thiamine biosynthesis by adenosine a tool for investigating this biosynthetic pathway.
1. Growth of Salmonella typhimurium LT2 in the presence of adenosine was shown to cause enormous synthesis of thiamine in washed-cell suspensions. 2. Evidence that this was due to de-repression and not an accumulation of precursors was obtained by using a mutant blocked in the biosynthesis of the thiazole moiety, which showed a similarly large synthesis of the pyrimidine of thiamine. 3. The specific requirements for a source of energy, nitrogen and sulphur were investigated, and indicated new synthesis in this system.
D001681:Biological Assay; D004794:Enzyme Repression / Q000187:drug effects*; D007778:Lactobacillus / Q000254:growth & development; D009705:Nucleosides / Q000494:pharmacology*; D011743:Pyrimidines / Q000032:analysis; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000254:growth & development; D013831:Thiamine / Q000096:biosynthesis*
D009705:Nucleosides; D011743:Pyrimidines; D013831:Thiamine