Immune response in vitro: independence of "activated" lymphoid cells.
Antibody formation against sheep erythrocytes by mouse spleen cells in vitro requires interactions among antigen-treated macrophages and lymphoid cells in cell culsters for only a finite time. During this critical period of interaction, lymphoid cells become "activated" and thereafter can develop into antibody-producing cells independently of native antigen, macrophages, and cell clusters.
D000818:Animals; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D046508:Culture Techniques; D004912:Erythrocytes / Q000276:immunology*; D008221:Lymphoid Tissue / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D051379:Mice; D012756:Sheep; D013154:Spleen / Q000276:immunology*; D013997:Time Factors