Endogenous encystment of Azotobacter vinelandii.
When young cells of Azotobacter vinelandii are impinged on membrane filters, washed free of carbon substrate, and placed on a mineral salts basal medium, the culture will proceed to encyst although at a slower rate than if n-butanol were supplied as a substrate. The endogenous cysts are depleted in polybeta-hydroxybutyrate and have a narrower intine but show an increased resistance to desiccation and are susceptible to lysis by chelating agents. Membrane-supported cells reveal details of the encystment process such as the formation of a zone within the capsule prior to exine formation and the early deposition of exine structures.
D001395:Azotobacter / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D001433:Bacteriolysis; D003470:Culture Media; D005374:Filtration; D005612:Freeze Drying; D008567:Membranes, Artificial; D008854:Microscopy, Electron
D003470:Culture Media; D008567:Membranes, Artificial