Intracellular protein breakdown in growing cells of Escherichia coli.
1. When Escherichia coli was grown exponentially in a defined medium at 35 degrees , the rate of protein breakdown was initially rapid, but decreased to 0.6%/hr. after about 30min. The latter rate was maintained for at least 3.5hr. 2. The initial rapid rate may have been due to the presence of a small protein fraction (about 1%) that was degraded with a half-life of 13min. 3. The rate of protein degradation was the same during balanced growth at low rates imposed in a bactogen. However, it increased during the period immediately after a decrease of the growth rate.
D001426:Bacterial Proteins / Q000378:metabolism*; D001431:Bacteriological Techniques; D002247:Carbon Isotopes; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D014633:Valine / Q000032:analysis / Q000378:metabolism
D001426:Bacterial Proteins; D002247:Carbon Isotopes; D000644:Quaternary Ammonium Compounds; D007930:Leucine; D014633:Valine