Soluble antigens for immunofluorescence detection of Histoplasma capsulatum antibodies.
Soluble antigens from Histoplasma capsulatum in the mycelial and yeast phase were purified by gel filtration, fixed onto paper discs, and employed in an indirect immunofluorescence procedure to detect antibody in sera from individuals infected with H. capsulatum. The elution patterns of crude histoplasmin passed through Sephadex G-200 revealed two minor peaks of protein showing immunofluorescence, complement fixing, and precipitating-antigen activity. A large peak containing the pigment and other low molecular weight materials showed no serological activity. A polysaccharide antigen obtained from fragmented, deproteinized yeast-phase cells was reactive in the fluorescent-antibody test but showed no antigen activity in complement fixation or precipitin tests. Although certain sera from culturally proven cases of blastomycosis, coccidioidomycosis, and cryptococcosis reacted with the purified Histoplasma antigens, preliminary evaluation indicated that the immunofluorescence technique may be of value as a screening procedure for the serodiagnosis of histoplasmosis.
D000906:Antibodies / Q000032:analysis; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000032:analysis; D003168:Complement Fixation Tests; D005455:Fluorescent Antibody Technique; D006658:Histoplasma / Q000276:immunology*; D006660:Histoplasmosis / Q000175:diagnosis; D011134:Polysaccharides / Q000032:analysis
D000906:Antibodies; D000941:Antigens; D011134:Polysaccharides