Single chain alkali resistance in hemoglobin Rainier: beta 145 tyrosine-histidine.
The mechanism of alkali resistance in hemoglobin Rainier, an adult human hemoglobin variant (beta 145 tyrosinie --> histidinie), has been investigated. Alkali denaturation kinetics, electrophoretic and hybridization study. and ultracentrifuge analysis provided evidence for a monomeric beta Rainiier chain resisting denaturation at alkaline pH. These data provide evidence for a previously unrecognized effect of a single amino acid substitution, that is, the change of the alkali denaturation properties of monomneric chains.
D000468:Alkalies; D000595:Amino Acid Sequence; D002499:Centrifugation, Density Gradient; D004586:Electrophoresis; D006455:Hemoglobins, Abnormal; D006639:Histidine; D008970:Molecular Weight; D011489:Protein Denaturation; D014443:Tyrosine
D000468:Alkalies; D006455:Hemoglobins, Abnormal; D014443:Tyrosine; D006639:Histidine