Differential effect of respiratory inhibitors on ergosterol synthesis by Saccharomyces cerevisiae during adaptation to oxygen.
The effect of different respiratory inhibitors on the ergosterol content of microaerobically grown non-proliferating yeast cultures was monitored during adaptation to oxygen. It was found that dinitrophenol, azide, and cyanide, which act on the mechanism of the respiratory chain, cause a marked stimulation of sterol production. Acriflavine and chloramphenicol, which affect the synthesis of the respiratory apparatus, caused a delay in the onset of ergosterol synthesis or a marked decrease in sterol content. The data obtained provide presumptive evidence that a component of sterol formation is synthesized on the 70S ribosomal system of the mitochondrion and induced in the presence of oxygen.
D000166:Acridines / Q000494:pharmacology; D000222:Adaptation, Physiological; D001386:Azides / Q000494:pharmacology; D002701:Chloramphenicol / Q000494:pharmacology; D003486:Cyanides / Q000494:pharmacology; D003864:Depression, Chemical; D004140:Dinitrophenols / Q000494:pharmacology; D008928:Mitochondria / Q000378:metabolism; D010100:Oxygen; D012270:Ribosomes / Q000378:metabolism; D012440:Saccharomyces / Q000032:analysis / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D013268:Stimulation, Chemical; D013997:Time Factors; D014807:Vitamin D / Q000032:analysis / Q000096:biosynthesis*
D000166:Acridines; D001386:Azides; D003486:Cyanides; D004140:Dinitrophenols; D014807:Vitamin D; D002701:Chloramphenicol; D010100:Oxygen