The release of prostaglandin E1 from the rat phrenic nerve-diaphragm preparation.
1. The release of prostaglandin E(1) from the rat phrenic nerve-diaphragm preparation during tetanic contraction of the muscle in response to nerve stimulation is reported. Tentative identification of the prostaglandin depended on solvent extraction column and thin-layer chromatography and parallel biological assay.2. Polar lipid substances were released from the preparation in the absence of nerve stimulation by (+)-tubocurarine and noradrenaline.3. The output on nerve stimulation was not abolished by (+)-tubocurarine, hemicholinium, bretylium or phenoxybenzamine added to the bath.4. The possible origin of these prostaglandins is discussed.
D000818:Animals; D001681:Biological Assay; D001949:Bretylium Compounds / Q000494:pharmacology; D002855:Chromatography, Thin Layer; D003964:Diaphragm / Q000378:metabolism*; D004558:Electric Stimulation; D005260:Female; D006426:Hemicholinium 3 / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D010643:Phenoxybenzamine / Q000494:pharmacology; D010791:Phrenic Nerve / Q000502:physiology*; D011453:Prostaglandins / Q000378:metabolism*; D051381:Rats; D014403:Tubocurarine / Q000494:pharmacology
D001949:Bretylium Compounds; D011453:Prostaglandins; D010643:Phenoxybenzamine; D006426:Hemicholinium 3; D014403:Tubocurarine; D009638:Norepinephrine