Plus and minus single-stranded DNA separately encapsidated in adeno-associated satellite virions.
Based on physical and chemical determinations, the mnolecular weight of the type 4 adeno-satellite virus is 5.4 X 10(6) daltons, and the virion contains 1.4 X 10(6) daltons of DNA. Denaturation and renaturation studies indicate that the viral genome is a single-stranded DNA molecule and that each virion contains either a minus or a plus strand. Upon extraction, the minus and plus strands unite to form double-stranded DNA molecules with no obvious excess of unpaired strands.
D000256:Adenoviridae / Q000032:analysis*; D002499:Centrifugation, Density Gradient; D004279:DNA, Viral / Q000302:isolation & purification*; D008854:Microscopy, Electron; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D012526:Satellite Viruses; D013057:Spectrum Analysis
D004279:DNA, Viral