Regulation of protein synthesis in zoospores of Blastocladiella.
The factors responsible for the regulation of protein synthesis in the zoospores of Blastocladiella emersonii were studied by means of cell fractionation and in vitro assays. Charged transfer ribonucleic acid (tRNA) and aminoacyl-tRNA synthetases were found both inside the membrane-bound, ribosomal nuclear cap, and in the extracap cytoplasm. Ribosomes isolated from zoospore nuclear caps in low salt buffer failed to support polyuridylic acid-dependent phenylalanine incorporation. After washing with high salt buffer, the cap ribosomes were equivalent in activity to similarly prepared plant ribosomes. Both the high-salt wash from cap ribosomes and the extracap supernatant fraction contained an unidentified material which inhibited aminoacyl-tRNA binding and peptide bond formation by ribosomes. Ribosomal binding of polyuridylic acid was not inhibited. Washed cap ribosomes supported very low incorporation rates without added messenger RNA, and were highly dependent upon added poly U for phenylalanine incorporation, indicating a low level of messenger in nuclear caps. It is concluded that enclosure of the ribosomes in the nuclear cap does not in itself prevent protein synthesis, and that the lack of activity may be due to the presence of a ribosome inhibitor.
D002247:Carbon Isotopes; D002499:Centrifugation, Density Gradient; D005658:Fungi / Q000032:analysis / Q000378:metabolism*; D008025:Ligases / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D010649:Phenylalanine / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D011485:Protein Binding; D012313:RNA / Q000096:biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012343:RNA, Transfer / Q000302:isolation & purification / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*; D013170:Spores / Q000378:metabolism*; D014500:Uracil Nucleotides / Q000378:metabolism
D002247:Carbon Isotopes; D012333:RNA, Messenger; D014500:Uracil Nucleotides; D010649:Phenylalanine; D012313:RNA; D012343:RNA, Transfer; D008025:Ligases; D008274:Magnesium; D011188:Potassium