Mutant of yeast sensitive to 2,6-diaminopurine.
A mutant of yeast sensitive to growth inhibition by 2,6-diaminopurine (2,6-DAP) was analyzed genetically and found to be a double mutant. One gene, dap, conferred approximately 30% sensitivity to the analogue. The other, slw, potentiated the inhibition such that the double mutant dap slw was inhibited 90%. The mutation dap conferred concomitant sensitivity to a number of other purine analogues. The activity of a purine phosphoribosyltransferase with 2,6-DAP in a strain carrying dap was found to be three times higher than in the wild type. It is inferred that the mutation alters the properties of a purine phosphoribosyltransferase. A possible mechanism for the effect of slw is also discussed.
D000225:Adenine / Q000494:pharmacology; D002498:Centrifugation; D005827:Genetics, Microbial; D005838:Genotype; D007042:Hypoxanthines / Q000494:pharmacology; D009154:Mutation; D010641:Phenotype; D011687:Purines / Q000494:pharmacology*; D014166:Transferases / Q000494:pharmacology; D014970:Xanthines / Q000494:pharmacology; D015003:Yeasts / Q000254:growth & development* / Q000302:isolation & purification
D007042:Hypoxanthines; D011687:Purines; D014970:Xanthines; D014166:Transferases; D000225:Adenine