The investigation of reactive sulphydryls in enzymes and their variants by starch gel electrophoresis. Studies on the human red cell peptidase variant Pep A 5-1.
D003538:Cystamine; D003545:Cysteine; D004586:Electrophoresis; D004912:Erythrocytes / Q000201:enzymology*; D005782:Gels; D005796:Genes; D005826:Genetics, Medical; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis; D005978:Glutathione; D006801:Humans; D007461:Iodoacetates; D007527:Isoenzymes / Q000032:analysis*; D008298:Maleates; D008623:Mercaptoethanol; D010447:Peptide Hydrolases / Q000032:analysis*; D013213:Starch; D013438:Sulfhydryl Compounds / Q000032:analysis*; D013451:Sulfonic Acids
D005782:Gels; D007461:Iodoacetates; D007527:Isoenzymes; D008298:Maleates; D013438:Sulfhydryl Compounds; D013451:Sulfonic Acids; D008623:Mercaptoethanol; D013213:Starch; D010447:Peptide Hydrolases; D005956:Glucose-6-Phosphate Isomerase; D005978:Glutathione; D003545:Cysteine; D003538:Cystamine