Double-bond requirement in a fatty acid desaturase mutant of Saccharomyces cerevisiae.
A fatty acid Delta(9)-desaturase mutant of yeast was analyzed to establish the specificity of the fatty acid structural requirement. Several double-bond-containing and substituted fatty acids were tested. It was concluded that the presence of a Delta(9)cis double bond is necessary for growth. The need for a specific chain length or a specific number of double bonds, or for both, is more flexible. Tracer-containing 16:1Delta(9)cis, 18:1Delta(9)cis, 18:2Delta(9,12)cis,cis and 18:3Delta(9,12,15) all cis revealed that each of these growth-supporting components, once taken up, was not converted into other fatty acids. Concentration effects on doubling time were also considered.
D002247:Carbon Isotopes; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism*; D008190:Lyases / Q000378:metabolism*; D008314:Malonates / Q000096:biosynthesis; D008566:Membranes / Q000502:physiology; D009154:Mutation; D012440:Saccharomyces / Q000201:enzymology* / Q000254:growth & development; D013997:Time Factors; D014316:Tritium
D002247:Carbon Isotopes; D005227:Fatty Acids; D008314:Malonates; D014316:Tritium; D008190:Lyases