Preparation of radioactive Tay-Sachs ganglioside labeled in the sialic acid moiety.
A procedure is described for the preparation of Tay-Sachs ganglioside specifically labeled in the sialic acid portion of the molecule. Rat brain gangliosides were labeled biosynthetically by the intracranial injection of N-acetyl-(3)H-D-mannosamine. Radioactive gangliosides were isolated and selectively degraded with bacterial neuraminidase and rat liver beta-galactosidase to Tay-Sachs ganglioside-(3)H. Radioactivity in the labeled product was confined to the N-acetyl-neuraminic acid portion of the molecule.
D000818:Animals; D001921:Brain / Q000378:metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D002855:Chromatography, Thin Layer; D003956:Dialysis; D005696:Galactosidases; D005732:Gangliosides / Q000096:biosynthesis / Q000302:isolation & purification; D006595:Hexosamines / Q000378:metabolism; D006801:Humans; D008064:Lipidoses / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008358:Mannose; D008722:Methods; D009438:Neuraminic Acids; D009439:Neuraminidase; D051381:Rats; D014316:Tritium; D014733:Vibrio / Q000201:enzymology
D005732:Gangliosides; D006595:Hexosamines; D009438:Neuraminic Acids; D014316:Tritium; D005696:Galactosidases; D009439:Neuraminidase; D008358:Mannose