Attempted isolation of a heparin proteoglycan from bovine liver capsule.
(1) Polysaccharides were isolated from bovine liver capsule by extraction with 2m-potassium chloride followed by precipitation from 0.8m-potassium chloride with cetylpyridinium chloride. Chondroitin sulphate was eliminated by digestion with hyaluronidase. The yield of heparin was approx. 40% of that obtained after extraction of the papain-digested tissue. (2) The macromolecular properties of the hyaluronidase-digested polysaccharide were studied by gel chromatography on Sephadex G-200 of the intact, as well as of the alkali-degraded, material. The results suggested the presence of single heparin chains in addition to a dermatan sulphate proteoglycan. (3) A purified heparin preparation was analysed for amino acids and neutral sugars. Xylose, galactose and serine were found in amounts corresponding to 0.1, 0.2, and 0.4 residue/polysaccharide chain (mol.wt. 7400), respectively. It is suggested that the isolated material had been degraded by a polysaccharidase with endo-enzyme properties.
D000818:Animals; D002417:Cattle; D002845:Chromatography; D005690:Galactose / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis*; D006493:Heparin / Q000032:analysis*; D006821:Hyaluronoglucosaminidase / Q000378:metabolism; D008099:Liver / Q000032:analysis*; D008970:Molecular Weight; D011134:Polysaccharides / Q000032:analysis* / Q000378:metabolism; D012694:Serine / Q000032:analysis; D014994:Xylose / Q000032:analysis
D006023:Glycoproteins; D011134:Polysaccharides; D012694:Serine; D006493:Heparin; D014994:Xylose; D006821:Hyaluronoglucosaminidase; D005690:Galactose