Ion metabolism in a Halobacterium. I. Influence of age of culture on intracellular concentrations.
Work is described on the changes in cell ions during growth of cultures of a species of Halobacterium isolated from the Dead Sea. Cell K concentration fell from 5.5 to 3.8 moles per kg cell water during the logarithmic phase of growth and maintained the latter value during the stationary phase (initial medium concentration, 7 mM). Cell Na and Cl followed a complex series of roughly parallel changes. The logarithmic phase ion concentrations were: Na, 1.0-2.3 moles/kg cell water; Cl, 2.3-3.7 moles/kg cell water. The final stationary phase values were: Na, 0.5 moles/kg cell water; Cl, 2.3-2.9 moles/kg cell water (medium NaCl concentration, 3.9 Molal). It is suggested that most of the K(+) is bound within the cytoplasm.
D002498:Centrifugation; D002712:Chlorides / Q000032:analysis / Q000378:metabolism*; D006217:Halobacterium / Q000254:growth & development / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010795:Phthalic Acids / Q000494:pharmacology; D011188:Potassium / Q000032:analysis / Q000378:metabolism*; D012964:Sodium / Q000032:analysis / Q000378:metabolism*
D002712:Chlorides; D010795:Phthalic Acids; D012964:Sodium; D011188:Potassium