Absorption of proteins and peptides in the far ultraviolet.
An absorption peak for the peptide bond at 187 nanometers has been confirmed; a protein assay at this wavelength allows quantitation of proteins in aqueous solution at concentrations between 0.1 and 25 micrograms per milliliter. Assays are conducted at 6 degrees C to take advantage of the reduction of end-absorption of water with temperature.
D005998:Glycine / Q000032:analysis*; D008722:Methods; D010455:Peptides / Q000032:analysis*; D012709:Serum Albumin / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis; D013053:Spectrophotometry; D013696:Temperature; D014466:Ultraviolet Rays
D010455:Peptides; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D005998:Glycine