Oligodeoxyribonucleotides: chemical synthesis in anhydrous base.
The sugar hydroxyl of a methoxytrityldeoxyribonucleoside reacts with a methoxytrityldeoxyribonucleoside phosphorofluoridate in dimethylformamide and potassium tertiary butoxide to yield the protected dinucleoside monophosphate. The reaction is fast and specific, and is used in a stepwise synthesis to prepare trinucleoside diphosphate and tetranucleoside triphosphate.
D002852:Chromatography, Ion Exchange; D004126:Dimethylformamide; D009705:Nucleosides; D009711:Nucleotides / Q000138:chemical synthesis*; D010087:Oxides
D009705:Nucleosides; D009711:Nucleotides; D010087:Oxides; D004126:Dimethylformamide