Isolation of monosomics in yeast.
Tetraploid cultures of Saccharomyces cerevisiae triplex (A/A/A/a) at several loci were sporulated. All the diploid spores are expected to be homozygous (A/A) or heterozygous (A/a) and, hence, to have the dominant phenotype. Cells lacking one of the chromosomes (monosomics) may show the recessive phenotype (a/-). Therefore, spores that grew on complete medium but failed to grow on the relevant synthetic single-omission media were presumed to be monosomic. These isolates were further characterized by sporulation, and several stable monosomics were established.
D002869:Chromosome Aberrations; D002874:Chromosome Mapping; D003470:Culture Media; D004171:Diploidy; D005808:Genes, Recessive; D005816:Genetic Complementation Test; D005827:Genetics, Microbial; D005838:Genotype; D009154:Mutation; D010641:Phenotype; D011123:Polyploidy; D012440:Saccharomyces / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D013170:Spores / Q000254:growth & development
D003470:Culture Media