Inside-out red cell membrane vesicles: preparation and purification.
Plasma membranes purified from human red cells were Converted into small vesicleS by disruption in alkaline buffer of low ionic strength. Most of these vesicles were inside-out. The presence of divalent cations prevented this inversion. The inside-out vesicles were separatcd from right-side-out vesicles by centrifugration to equilibrium in dextran density gradients.
D002462:Cell Membrane / Q000033:anatomy & histology*; D002499:Centrifugation, Density Gradient; D004912:Erythrocytes / Q000166:cytology*; D005613:Freeze Etching; D006651:Histocytochemistry; D006801:Humans; D008722:Methods; D008854:Microscopy, Electron