Metabolism of some polyols by Rhizobium meliloti.
The utilization of d-mannitol, d-arabitol, and d-sorbitol by Rhizobium meliloti was studied in extracts from mannitol-grown cells. Two different polyol dehydrogenases were induced by any of these polyols: (i) a nicotinamide adenine dinucleotide (NAD)-arabitol dehydrogenase and (ii) a NAD-sorbitol dehydrogenase, whereas polyol phosphate dehydrogenases were absent. d-Arabitol dehydrogenase was observed to act on both d-arabitol and d-mannitol, but d-sorbitol dehydrogenase acted specifically on d-sorbitol. d-Arabitol was oxidized to d-xylulose, d-mannitol and d-sorbitol were oxidized to d-fructose. An adenosine triphosphate-linked hexokinase which acts on d-fructose and absence of hexose isomerase were also detected in this organism.
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002474:Cell-Free System; D002848:Chromatography, DEAE-Cellulose; D002854:Chromatography, Paper; D004790:Enzyme Induction; D005632:Fructose / Q000096:biosynthesis; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007535:Isomerases / Q000378:metabolism; D008353:Mannitol / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism; D012231:Rhizobium / Q000201:enzymology / Q000378:metabolism*; D013012:Sorbitol / Q000378:metabolism*; D013053:Spectrophotometry; D014994:Xylose / Q000096:biosynthesis
D005632:Fructose; D008353:Mannitol; D013012:Sorbitol; D014994:Xylose; D000429:Alcohol Oxidoreductases; D005954:Glucosephosphate Dehydrogenase; D010770:Phosphotransferases; D006593:Hexokinase; D007535:Isomerases