Physiological advantage of the mechanism of the tryptophan synthetase reaction.
Indole-accumulating and indole-utilizing tryptophan synthetase mutants of Saccharomyces cerevisiae were found to form complementing diploids with unusual growth properties. These strains grew at nearly the wild-type exponential rate, but only after an abnormally prolonged lag period of a duration that depended on the initial cell density. The exaggerated lag was reduced by adding low concentrations of indole in the medium. The growth properties were interpreted to be a consequence of the inability of these strains to synthesize tryptophan by the normal mechanism.
D002247:Carbon Isotopes; D003124:Colorimetry; D003470:Culture Media; D004171:Diploidy; D005827:Genetics, Microbial; D006836:Hydro-Lyases / Q000378:metabolism*; D007211:Indoles / Q000378:metabolism; D009154:Mutation; D012440:Saccharomyces / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D014364:Tryptophan / Q000096:biosynthesis
D002247:Carbon Isotopes; D003470:Culture Media; D007211:Indoles; D014364:Tryptophan; D006836:Hydro-Lyases