Determination of alpha-tocopherol in erythrocytes by gas-liquid chromatography.
A method is described for the determination of submicrogram amounts of alpha-tocopherol in 0.5 ml of packed erythrocytes. The alpha-tocopherol in a lipid extract is oxidized to alpha-tocopherylquinone which is separated by thin-layer chromatography, eluted, and quantitated by gas-liquid chromatography. Calculation is based on the recovery of added alpha-tocopherol-(3)H. Erythrocytes from stock rats had an average alpha-tocopherol concentration of 344 micro g/100 ml of packed cells, while for human cells the average was 235 micro g/100 ml. The ratio of red cell to plasma alpha-tocopherol was 0.482 for rat blood, and 0.244 for human blood.
D000818:Animals; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D004912:Erythrocytes / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D008722:Methods; D010084:Oxidation-Reduction; D010949:Plasma / Q000032:analysis; D011809:Quinones / Q000032:analysis; D011854:Radiochemistry; D051381:Rats; D012737:Sex Factors; D014316:Tritium; D014810:Vitamin E / Q000097:blood*
D011809:Quinones; D014316:Tritium; D014810:Vitamin E