Factors affecting the glycosphingolipid composition of murine tissues.
The effects of genetic strain, sex, age, and pathological state on the distribution and concentration of glycosphingolipids in mouse kidneys and livers were studied. The concentrations of glycosphingolipids and phospholipids in the kidneys and livers of different strains were compared. The major glycosphingolipid in the kidneys of male and female BALB/c, C3H/He, C57/BL, A, and C57xA (F(1) hybrid) mice was a sulfatide, monoglycosyl-(3-sulfate) ceramide; monoglycosyl ceramide was the major component in livers. The kidneys of males of all strains contained significant amounts of diglycosyl ceramide, but those of females contained, at most, only traces. Glycosphingolipid concentration in the male kidneys of C57/BL and C57xA (F(1) hybrid) was much higher than in the female and was also much higher than in the male kidneys of C3H/He, BALB/c, and A strains. The kidneys of "old" (36 wk) male and female C3H/He mice contained much higher proportions of monoglycosyl ceramide than 10-12-wk-old adults. The distributions of glycosphingolipids in kidneys of female C3H/He mice with BP8 ascites tumors and male C57xA (F(1) hybrid) mice with EL4 ascites tumors differed from those in the normal mice. An unknown lipid, present in all glycosphingolipid extracts from kidney and liver, was tentatively identified as cholesterol sulfate.
D000367:Age Factors; D000818:Animals; D002554:Cerebrosides / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D002855:Chromatography, Thin Layer; D005260:Female; D005823:Genetics; D006017:Glycolipids / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D007942:Leukemia, Experimental / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D012513:Sarcoma, Experimental / Q000378:metabolism; D012737:Sex Factors; D013045:Species Specificity; D013464:Sulfuric Acids / Q000378:metabolism
D002554:Cerebrosides; D006017:Glycolipids; D010743:Phospholipids; D013464:Sulfuric Acids; D002784:Cholesterol