Some factors influencing the release of 5-hydroxyindol-3-ylacetic acid in the forebrain.
1. Electrical stimulation of the mid-brain raphé in anaesthetized adrenalectomized rats produced a significant decrease in the forebrain content of 5-hydroxytryptamine (5-HT) and an increase in the concentration of 5-hydroxyindol-3-ylacetic acid (5-HIAA).2. Stimulation of peripheral sensory nerves did not influence either the forebrain content of 5-HIAA or the efflux of 5-HIAA from the cerebral cortex.3. Probenecid (200 mg/kg) caused a twofold increase in 5-HIAA content of the rat's forebrain, while the efflux of 5-HIAA from the cerebral cortex remained unchanged.4. Stimulation of the mid-brain raphé in animals pretreated with probenecid does not produce the rise in the forebrain levels of 5-HIAA seen in stimulated untreated controls and does not affect the efflux of 5-HIAA from the cerebral cortex.5. In preliminary experiments, lysergic acid diethylamide (LSD 25) substantially reduced and/or prevented the increase in the release of 5-HIAA in the forebrain observed in untreated animals with raphé stimulation.
D000315:Adrenalectomy; D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism; D004558:Electric Stimulation; D006897:Hydroxyindoleacetic Acid / Q000032:analysis / Q000378:metabolism*; D008238:Lysergic Acid Diethylamide / Q000494:pharmacology; D008297:Male; D008636:Mesencephalon / Q000502:physiology; D010525:Peripheral Nerves / Q000502:physiology; D011339:Probenecid / Q000494:pharmacology; D051381:Rats; D012701:Serotonin / Q000032:analysis; D013687:Telencephalon / Q000032:analysis / Q000378:metabolism*
D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D008238:Lysergic Acid Diethylamide; D011339:Probenecid