Steroid metabolism in the rabbit. Biliary and urinary excretion of metabolites of [4-14C] cortisone.
1. [4-(14)C]Cortisone was administered to anaesthetized male and female New Zealand White rabbits as a single injection or as a 45-60min infusion. 2. The method of administration of the steroid did not significantly affect the total excretion of radioactivity in bile and urine [83.8+/-10.8%(s.d.)]. 3. The mean ratio of metabolites in urine to those in bile was 0.97+/-0.23% (range 0.64-1.3). 4. When bile and urine samples were hydrolysed successively by beta-glucuronidase, cold acid and hot acid, neutral metabolites extracted by ethyl acetate-ether were found mainly after hydrolysis by beta-glucuronidase. 5. An approximately equal proportion of the dose was converted into substances not extractable from alkaline aqueous solution after hydrolysis.
D000818:Animals; D001646:Bile / Q000032:analysis*; D002247:Carbon Isotopes; D003348:Cortisone / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism* / Q000652:urine*; D005260:Female; D005966:Glucuronidase / Q000378:metabolism; D008297:Male; D011817:Rabbits
D002247:Carbon Isotopes; D005966:Glucuronidase; D003348:Cortisone