Heme requirement for reproduction of a free-living nematode.
The free-living hermaphroditic nematode Caernorhabditis briggsae has a nutritional requirement for heme. The organism can be subcultured repeatedly in a chemically defined axenic medium that contains autoclaved bacterial cells (Escherichia coli) and sterols if a hemeprotein-containing fraction from liver is present. Pure myoglobin, hemoglobin, cytochrome c, and hemin, respectively, can substitute effectively for the liver fraction.
D000818:Animals; D003470:Culture Media; D003580:Cytochromes / Q000494:pharmacology; D006418:Heme / Q000494:pharmacology*; D006454:Hemoglobins / Q000494:pharmacology; D008099:Liver; D009211:Myoglobin / Q000494:pharmacology; D009348:Nematoda / Q000254:growth & development*; D012098:Reproduction / Q000187:drug effects*; D014020:Tissue Extracts / Q000494:pharmacology
D003470:Culture Media; D003580:Cytochromes; D006454:Hemoglobins; D009211:Myoglobin; D014020:Tissue Extracts; D006418:Heme