Isolation and zonal fractionation of metaphase chromosomes from human diploid cells.
A simple method for isolating large quantities of human metaphase chromosomes has been developed. Fractionation of chromosomes from peripheral lymphocytes on a large scale is accomplished by velocity sedimentation in an A-XII zonal rotor.
D002499:Centrifugation, Density Gradient; D002501:Centrifugation, Zonal; D002875:Chromosomes / Q000032:analysis*; D004171:Diploidy; D006801:Humans; D008214:Lymphocytes / Q000032:analysis; D008722:Methods; D008938:Mitosis