Zymogen activation as a sensitive enzyme-amplifying assay for a protease with tryptic specificity.
A protease, ;trypsinogenase', secreted in small amounts by the sea-urchin blastula, is assayed in two steps as an example of enzyme-amplifying kinetics. In reaction 1 the trypsinogenase catalyses the activation of trypsinogen to trypsin. In reaction 2 the trypsin catalyses the hydrolysis of N-alpha-toluene-p-sulphonylarginine methyl ester, at a rate that is linear with trypsinogenase concentration over a 20-fold range. Results are reproducible within a batch of zymogen, but each batch requires a separate standard curve.
D000818:Animals; D004447:Echinodermata; D004625:Embryo, Nonmammalian / Q000201:enzymology; D004792:Enzyme Precursors; D004952:Esters; D010447:Peptide Hydrolases / Q000032:analysis*; D013268:Stimulation, Chemical; D014357:Trypsin; D014362:Trypsinogen
D004792:Enzyme Precursors; D004952:Esters; D014362:Trypsinogen; D010447:Peptide Hydrolases; D014357:Trypsin