Automated continuous culture of mammalian cells in suspension.
A system has been developed for the continuous culture of mammalian cells in suspension. The system maintains constant cell concentrations (monitored as the degree of light scattering) over a wide range of previously selected values by automatic additions of known amounts of medium and simultaneous withdrawals of equal volumes of cell suspension.
D000818:Animals; D001331:Automation; D002452:Cell Count; D046508:Culture Techniques / Q000295:instrumentation*; D006367:HeLa Cells / Q000254:growth & development