Fluorimetric determination of sphingosine and its application to natural mixtures of glycosphingolipids.
A sensitive estimation of sphingosine, by measurement of the fluorescence of a complex formed with 1-naphthylamino-4-sulfonic acid, is described. The practical range is 5-35 nmoles sphingosine. The method is used to estimate, in terms of sphingosine, amounts of ceramide and glycosphingolipids. The isolation of microamounts (5-30 micro g) of individual glycosphingolipids from a mixture, and their quantitative estimation is described. The percentage composition of a glycosphingolipid mixture from the kidneys of adult C57/BL male mice is given.
D000605:Amino Alcohols / Q000032:analysis*; D000818:Animals; D002554:Cerebrosides / Q000032:analysis; D002855:Chromatography, Thin Layer; D005453:Fluorescence; D005470:Fluorometry; D006017:Glycolipids / Q000032:analysis*; D007668:Kidney / Q000032:analysis*; D008168:Lung / Q000032:analysis; D008297:Male; D008722:Methods; D051379:Mice; D009281:Naphthalenes; D013451:Sulfonic Acids; D013552:Swine
D000605:Amino Alcohols; D002554:Cerebrosides; D006017:Glycolipids; D009281:Naphthalenes; D013451:Sulfonic Acids