Doubls-helical polynucleotides: immunochemical recognition of differing conformations.
Rabbit antibodies to double-helical RNA react by complement fixation with synthetic or natural double-strand RNA but not with native DNA. In turn, human (from systemic lupus erythematosus patients) antibodies to native DNA do not react with double-strand RNA. Both types of antibodies show cross-reactions (from 1 percent to 50 percent) with RNA-DNA hybrids, but antibodies to the hybrids do not react at all with double-strand RNA or with native DNA. Antibodies to polydeoxyguanylate.polydeoxycytidylate also failed to react with native DNA.
D000227:Adenine Nucleotides; D000818:Animals; D000937:Antigen-Antibody Reactions; D003168:Complement Fixation Tests; D003429:Cross Reactions; D003597:Cytosine Nucleotides; D004247:DNA; D006150:Guanine Nucleotides; D006824:Hybridization, Genetic; D009691:Nucleic Acid Denaturation; D011119:Polynucleotides; D012313:RNA; D012367:RNA, Viral; D011817:Rabbits; D012087:Reoviridae; D014500:Uracil Nucleotides
D000227:Adenine Nucleotides; D003597:Cytosine Nucleotides; D006150:Guanine Nucleotides; D011119:Polynucleotides; D012367:RNA, Viral; D014500:Uracil Nucleotides; D012313:RNA; D004247:DNA