Temporal and hormonal control of beta-1,3-glucanase in Phaseolus vulgaris L.
The endo-beta-1, 3-glucanase (beta-1, 3-glucan 3-glucanhydrolase, EC 3.2.1.6) extracted from Phaseolus vulgaris L. cv. Red Kidney had a pH optimum of 5 and a temperature optimum of 50 C. Excision of plant tissue resulted in an increase in beta-1, 3-glucanase activity after a 6-hour lag period. The increase could be prevented by indole-3-acetic acid, gibberellic acid, and cytokinins. Ethylene (half-maximal concentration = 0.1 microliter/liter) promoted the synthesis of beta-1, 3-glucanase, and 10% CO(2) overcame some of the ethylene effect. Cycloheximide prevented the induction of beta-1, 3-glucanase, but actinomycin D and chromomycin A(3) had only a partial effect. The amount of callose in sieve tube cells correlated with levels of beta-1, 3-glucanase, suggesting that this enzyme played a role in the degradation of beta-1, 3-glucans.
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D005030:Ethylenes / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000494:pharmacology; D010944:Plants / Q000201:enzymology*; D011134:Polysaccharides / Q000378:metabolism
D000903:Antibiotics, Antineoplastic; D005030:Ethylenes; D010937:Plant Growth Regulators; D011134:Polysaccharides; D003609:Dactinomycin; D003513:Cycloheximide; D006026:Glycoside Hydrolases