Estimation of platelet adhesiveness on whole blood and platelet-rich plasma.
Platelet adhesive tests were carried out in parallel on whole blood and platelet-rich plasma. A column of glass microspherules was used and adenosine diphosphate (ADP) was added to the platelet-rich plasma before passage through the column. Preliminary observations were made to show the effect of the containers and the anticoagulant used. The influence on platelets when stored at 4 degrees C and standing or mixing at room temperature was also assessed. The apparatus used to measure adhesiveness was designed to produce a constant rate of flow through a standard column of glass microspherules. Using both methods, adhesiveness was studied on 70 healthy blood donors. The result showed a significant correlation between the level of adhesiveness found with each technique.
D000227:Adenine Nucleotides / Q000494:pharmacology; D000328:Adult; D000368:Aged; D000925:Anticoagulants; D001772:Blood Cell Count; D001792:Blood Platelets; D001793:Blood Preservation; D002951:Citrates; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010949:Plasma; D010973:Platelet Adhesiveness; D013696:Temperature; D013997:Time Factors
D000227:Adenine Nucleotides; D000925:Anticoagulants; D002951:Citrates