Gas--liquid chromatography--mass spectrometry of synthetic ceramides containing 2-hydroxy acids.
Ceramides containing either sphingosine or sphinganine and one of the 2-hydroxy acids, 14h:0, 16h:0, 18h:0, 20h:0, 22h:0, 24h:0, and 26h:0 were prepared and separated by gas chromatography as the 1,3,2'-tri-O-tri-methylsilyl derivatives. Mass spectrometric analyses of these derivatives showed that the ions formed on electron impact can be used to determine unequivocally the structures of the long-chain base and the fatty acid residue in the ceramide. Proposed structures of ions and the mechanisms of reaction of their formation are supported by mass spectra of homologous derivatives, by deuterium labeling experiments, and by high-resolution on mass spectrometry.
D000605:Amino Alcohols / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D003903:Deuterium; D005227:Fatty Acids / Q000032:analysis*; D008722:Methods; D008961:Models, Structural; D012825:Silicon; D013057:Spectrum Analysis; D013237:Stereoisomerism
D000605:Amino Alcohols; D005227:Fatty Acids; D003903:Deuterium; D012825:Silicon