Countercurrent chromatography: liquid-liquid partition chromatography without solid support.
The liquid-liquid partition chromatographic system reported here involves a long helix of narrow-bore tubing. When the coiled tube is filled with one phase of a two-phase system and fed with the other phase, phase-interchange takes place in each turn of the coil, leaving a segment of the former phase as the stationary phase. Consequently, solutes present in either phase are subjected to a multistep partition process. The column efficiency, estimated on a separation of dinitrophenyl amino acids, is comparable to that of gas chromatography.
D000596:Amino Acids / Q000032:analysis; D002845:Chromatography / Q000295:instrumentation*; D003377:Countercurrent Distribution; D004140:Dinitrophenols / Q000032:analysis; D008722:Methods
D000596:Amino Acids; D004140:Dinitrophenols