The amino acid sequence of Phaseolus aureua L. (mung-bean) cytochrome c.
The amino acid sequence of Phaseolus aureus L. (mung-bean) cytochrome c has been determined. The molecule consists of a single polypeptide chain of 111 amino acid residues and is homologous with other mitochondrial cytochromes c. Comparison with the amino acid sequence of wheat-germ cytochrome c (Stevens, Glazer & Smith, 1967) shows 14 differences. On alignment with mammalian cytochromes c, mung-bean cytochrome c has an N-acetylated ;tail' of eight amino acid residues similar to that found in wheat-germ cytochrome c. Of the 22 positions in wheat-germ cytochrome c that contain amino acid residues unique to these positions, 20 were found to contain the same ones in mung-bean cytochrome c. The in-N-trimethyl-lysine residues reported for wheat-germ cytochrome c (Delange, Glazer & Smith, 1969) in positions 72 and 86 were also found in these positions in mung-bean cytochrome c. The sequence was determined from 3mumol, by using chymotryptic and tryptic peptides which were analysed by the ;dansyl'-Edman method (Gray & Hartley, 1963a), with confirmation by amino acid analysis.
D000595:Amino Acid Sequence; D002918:Chymotrypsin; D003580:Cytochromes / Q000032:analysis*; D008928:Mitochondria; D010455:Peptides; D010944:Plants / Q000032:analysis*; D013045:Species Specificity; D014908:Triticum; D014357:Trypsin
D003580:Cytochromes; D010455:Peptides; D002918:Chymotrypsin; D014357:Trypsin