Metabolism of a glutathione conjugate of 2-hydroxyoestradiol by rat liver and kidney preparations in vitro.
Adult male rat liver and kidney preparations were incubated with (2-hydroxyoestradiol-1-yl)[(35)S]glutathione. The glutamic acid and glycine residues were removed by enzymes present in the kidney microsomal fraction; the liver preparations had no effect. The resulting 2-hydroxyoestradiol-cysteine conjugate was acetylated at the alpha-amino group by both liver and kidney homogenates fortified with acetyl-coenzyme A, but not significantly in the absence of this coenzyme, or by liver or kidney slices. These results suggest that an oestrogen-glutathione conjugate, if formed in vivo, would be converted into the corresponding mercapturic acid before excretion.
D000818:Animals; D002854:Chromatography, Paper; D003545:Cysteine / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D005998:Glycine / Q000378:metabolism; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D011422:Propionates / Q000378:metabolism; D051381:Rats; D013057:Spectrum Analysis; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013460:Sulfur Isotopes
D005971:Glutamates; D007773:Lactates; D011422:Propionates; D013438:Sulfhydryl Compounds; D013460:Sulfur Isotopes; D004958:Estradiol; D005978:Glutathione; D003545:Cysteine; D005998:Glycine