Changes in extrinsic fluorescence in squid axons during voltage-clamp.
Fluorescence changes in squid axons were examined after staining with rhodamine B, pyronin B, or 8-anilinonaphthalene-1-sulfonate by intracellular application. Gradual changes in fluorescence were detected during both hyperpolarizing and depolarizing voltage-clamp pulses. Abrupt changes were often observed at the onset and at the end of voltage-clamp. Possible sources of artifact in optical measurements of this type and some implications of the findings are discussed.
D000818:Animals; D001369:Axons / Q000502:physiology*; D005453:Fluorescence; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D008564:Membrane Potentials; D008974:Mollusca; D009281:Naphthalenes; D010477:Perfusion; D011189:Potassium Chloride; D000644:Quaternary Ammonium Compounds; D012623:Seawater; D013451:Sulfonic Acids; D014966:Xanthenes
D005456:Fluorescent Dyes; D009281:Naphthalenes; D000644:Quaternary Ammonium Compounds; D013451:Sulfonic Acids; D014966:Xanthenes; D011189:Potassium Chloride