Viability and infectivity of measles virus-infected nervous tissue frozen in cryoprotective medium.
Measles virus-infected hamster brain tissue remained viable when frozen slowly in medium containing dimethyl sulfoxide. This procedure was essential for isolation of neurotropic measles virus from tissue specimens stored frozen.
D000818:Animals; D001921:Brain; D006224:Cricetinae; D003451:Cryoprotective Agents; D003470:Culture Media; D003937:Diagnosis, Differential; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004195:Disease Models, Animal; D004660:Encephalitis / Q000175:diagnosis; D005615:Freezing; D008457:Measles / Q000175:diagnosis; D008459:Measles virus / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity; D014021:Tissue Preservation; D014357:Trypsin
D003451:Cryoprotective Agents; D003470:Culture Media; D014357:Trypsin; D004121:Dimethyl Sulfoxide