Defect in DNA synthesis in skin of patients with xeroderma pigmentosum demonstrated in vivo.
Exposure of normal human skin in vivo to ultraviolet irradiation at wavelengths shorter than 320 nanometers stimtulated an unscheduled DNA synthesis in all of the cell layers of the epidermis and in the upper dermnial fibrocytes. The skin of patients with xeroderma pigmentosum did not show this response. correlation of these findings with previous tissue culture studies suggests that the defect in repair of the damaged DNA in xeroderma cells occurs in vivo as well as in vitro.
D001345:Autoradiography; D004247:DNA / Q000096:biosynthesis*; D006801:Humans; D011831:Radiation Genetics; D012867:Skin / Q000378:metabolism* / Q000528:radiation effects; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014466:Ultraviolet Rays; D014983:Xeroderma Pigmentosum / Q000378:metabolism*
D014316:Tritium; D004247:DNA; D013936:Thymidine