Purified rabies vaccine (suckling rat brain origin).
A 10% suckling rat brain rabies vaccine free from encephalitogenic activity was prepared and inactivated with 1:8,000 beta-propiolactone (BPL), or ultraviolet light, or a combination of ultraviolet light and BPL, or 1% phenol. Potency was excellent in all samples, with the exception of the phenolized product which was marginal. A purified suckling rat brain (SRB) vaccine prepared by zonal centrifugation and inactivated with 1:8,000 BPL contained about 0.01 the amount of protein nitrogen of the unpurified 10% SRB vaccine. This purified product passed the National Institutes of Health potency test for rabies vaccine after administration of a quantity equivalent to a standard 10% brain suspension.
D000818:Animals; D001921:Brain; D002499:Centrifugation, Density Gradient; D002501:Centrifugation, Zonal; D006168:Guinea Pigs; D006571:Heterocyclic Compounds; D007783:Lactones; D051379:Mice; D009584:Nitrogen / Q000032:analysis; D010636:Phenols; D011422:Propionates; D011819:Rabies Vaccines / Q000032:analysis / Q000302:isolation & purification* / Q000592:standards; D011820:Rabies virus; D051381:Rats; D013395:Sucrose; D014466:Ultraviolet Rays
D006571:Heterocyclic Compounds; D007783:Lactones; D010636:Phenols; D011422:Propionates; D011819:Rabies Vaccines; D013395:Sucrose; D009584:Nitrogen