Partial purification of an extracellular leptospiral lipase.
Sequential procedures involving ethanol precipitation, ultracentrifugation, and molecular sieve chromatography in deoxycholate permitted 140-fold purification of a leptospiral lipase.
D011232:Chemical Precipitation; D002845:Chromatography; D002850:Chromatography, Gel; D000431:Ethanol; D005612:Freeze Drying; D007919:Leptospira / Q000201:enzymology*; D008049:Lipase / Q000302:isolation & purification*; D008722:Methods; D008970:Molecular Weight; D014461:Ultracentrifugation
D000431:Ethanol; D008049:Lipase