Desmosterol as the major sterol in L-cell mouse fibroblasts grown in sterol-free culture medium.
The principal sterol synthesized by L-cell mouse fibroblasts is desmosterol. Cholesterol was not detected in these cells when they were grown in a sterol-free culture medium. These findings indicate that, in cells, cholesterol can be replaced by desmosterol. Sterol analyses of six other tissue culture cell lines revealed cholesterol synthesis.
D000085:Acetates / Q000378:metabolism; D000818:Animals; D002247:Carbon Isotopes; D002277:Carcinoma; D002776:Cholestanes / Q000032:analysis / Q000096:biosynthesis*; D002784:Cholesterol / Q000096:biosynthesis; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D003470:Culture Media; D066298:In Vitro Techniques; D007739:L Cells / Q000378:metabolism*; D007822:Laryngeal Neoplasms; D008168:Lung; D051379:Mice; D013261:Sterols / Q000032:analysis / Q000096:biosynthesis*
D000085:Acetates; D002247:Carbon Isotopes; D002776:Cholestanes; D003470:Culture Media; D013261:Sterols; D002784:Cholesterol