Malaria resistance: artificial induction with a partially purified plasmodial fraction.
Plasmodium berghei were released from mouse erythrocytes by passage through a French pressure cell. The released organisms were washed and disintegrated; the soluble portion was chromatographed on a Sephadex (G-200) column. The void-volume eluate contained an erythrocyte-free plasmodial fraction which behaved as a vaccine, preventing parasitemia, anemia, and death in mice subsequently challenged with living Plasmodium berghei.
D000818:Animals; D002852:Chromatography, Ion Exchange; D008288:Malaria / Q000276:immunology* / Q000517:prevention & control; D051379:Mice; D010961:Plasmodium / Q000276:immunology / Q000302:isolation & purification*; D014612:Vaccines / Q000627:therapeutic use*
D014612:Vaccines