Evidence for a single-stranded adenovirus-associated virus genome: isolation and separation of complementary single strands.
Single-stranded adenovirus-associated virus type 2 deoxyribonucleic acid (AAV-2 DNA) has been isolated from the virion after enzymatic pretreatment of the particles by heating at 53 C for 1 hr in 0.015 m NaCl plus 0.0015 m sodium citrate in the presence of 1% sodium dodecyl sulfate. Double-stranded AAV-2 DNA present as a marker is not denatured by this treatment. AAV-2 single-stranded DNA is composed of two complementary species which can be separated in neutral CsCl when 5-bromodeoxyuridine has been substituted for thymidine in the DNA. The present report is the first documented instance of the separation of complementary strands of an animal virus DNA.
D000256:Adenoviridae / Q000254:growth & development / Q000302:isolation & purification; D000468:Alkalies; D001973:Bromodeoxyuridine; D002247:Carbon Isotopes; D002277:Carcinoma; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D002951:Citrates; D004279:DNA, Viral / Q000302:isolation & purification*; D005827:Genetics, Microbial; D006358:Hot Temperature; D006801:Humans; D008967:Molecular Biology; D009062:Mouth Neoplasms; D009691:Nucleic Acid Denaturation; D012964:Sodium; D012965:Sodium Chloride; D013395:Sucrose; D013431:Sulfates; D013936:Thymidine; D014316:Tritium
D000468:Alkalies; D002247:Carbon Isotopes; D002712:Chlorides; D002951:Citrates; D004279:DNA, Viral; D013431:Sulfates; D014316:Tritium; D002586:Cesium; D012965:Sodium Chloride; D013395:Sucrose; D012964:Sodium; D001973:Bromodeoxyuridine; D013936:Thymidine