The separation of multiple forms of housefly 1,1,1-trichloro-2,2-bis-(p-chlorophenyl)ethane (DDT) dehydrochlorinase from glutathione S-aryltransferase by electrofocusing and electrophoresis.
Electrophoresis in a sucrose gradient at pH values between 5 and 8 separated housefly DDT [1,1,1-trichloro-2,2-bis-(p-chlorophenyl)ethane] dehydrochlorinase into two major fractions. GSH S-aryltransferase under similar conditions migrated as a single peak of activity. Separation of housefly homogenates or partially purified enzyme preparations by electrofocusing in a natural pH gradient also showed the presence of multiple forms of DDT dehydrochlorinase.
D000818:Animals; D002850:Chromatography, Gel; D003634:DDT; D004586:Electrophoresis; D005978:Glutathione; D006793:Houseflies / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D008190:Lyases / Q000032:analysis*; D014166:Transferases / Q000032:analysis*
D003634:DDT; D014166:Transferases; D008190:Lyases; D005978:Glutathione