Cell wall glucans of the yeast and mycelial forms of Paracoccidioides brasiliensis.
Glucans were isolated from the cell wall of the yeast (Y) and mycelial (M) forms of Paracoccidioides brasiliensis. The alkali-soluble glucan of the Y form had properties of alpha-1,3-glucan. The alkali-insoluble glucan of the M form was identified as a beta-glucan which contains a beta-(1 --> 3)-glycosidic linkage by infrared absorption spectrum, by effect of beta-1,3-glucanase, and by partial acid hydrolysis. The alkali-soluble glucans of the M form were a mixture of alpha- and beta-glucans and the ratio of alpha- to beta-glucan was variable, depending on the preparations.
D000042:Absorption; D001758:Blastomyces / Q000201:enzymology; D002473:Cell Wall / Q000032:analysis*; D002482:Cellulose / Q000032:analysis*; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D003124:Colorimetry; D005658:Fungi / Q000032:analysis* / Q000201:enzymology; D006026:Glycoside Hydrolases / Q000378:metabolism; D007259:Infrared Rays; D011110:Polymorphism, Genetic; D012995:Solubility; D013053:Spectrophotometry; D013696:Temperature
D002482:Cellulose; D006026:Glycoside Hydrolases