Puffing and histone acetylation in polytene chromosomes.
Fixation with picric acid or formaldehyde retains incorporated [(3)H]acetate which is lost after fixation with ethanol and acetic acid. Unlike [(3)H]uridine, [(3)H]acetate is diffusely incorporated into polytene chromosomes, and not preferentially into existing or newly induced puffs. It is suggested that puff formation does not include an acetylation of histones.
D000085:Acetates / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D002875:Chromosomes / Q000378:metabolism*; D004330:Drosophila; D005557:Formaldehyde; D006657:Histones / Q000378:metabolism*; D006851:Hydrochloric Acid; D012313:RNA / Q000096:biosynthesis; D012469:Salivary Glands / Q000166:cytology / Q000378:metabolism; D014316:Tritium
D000085:Acetates; D006657:Histones; D014316:Tritium; D005557:Formaldehyde; D012313:RNA; D006851:Hydrochloric Acid