Solid-pse radioimmunoassay of protein biosynthesis.
Solid-phase radioimmunoassay was used for measuring the rate of radioisotope incorporation into a single protein species. Radioactive antigen was measured by its binding to specific antiserum covalently linked to insoluble bromoacetylcellulose. The insoluble antigen-antibody complex was collected on filters for counting. The assay is performed in about 4 hours as compared with several days for methods based on the precipitin reaction.
D000818:Animals; D000936:Antigen-Antibody Complex; D002482:Cellulose; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007136:Immunoglobulins / Q000096:biosynthesis; D007456:Iodine Isotopes; D007700:Kinetics; D008722:Methods; D011485:Protein Binding; D011817:Rabbits; D011863:Radioimmunoassay
D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D007136:Immunoglobulins; D007456:Iodine Isotopes; D002482:Cellulose