Simple selection for end-product inhibitor-insensitive mutants in yeast.
Trifluoroleucine-resistant yeast mutants with an end-product-insensitive alpha-isopropylmalate synthetase are dominant in diploids. This property provides a simple screen for their detection.
D000217:Acyltransferases / Q000378:metabolism*; D003433:Crosses, Genetic; D003998:Dicarboxylic Acids / Q000378:metabolism; D004171:Diploidy; D004352:Drug Resistance, Microbial; D005827:Genetics, Microbial; D007930:Leucine / Q000494:pharmacology; D008025:Ligases / Q000378:metabolism*; D009154:Mutation; D012440:Saccharomyces / Q000187:drug effects / Q000201:enzymology; D014631:Valerates / Q000378:metabolism
D003998:Dicarboxylic Acids; D014631:Valerates; D000217:Acyltransferases; D008025:Ligases; D007930:Leucine