Equilibrium density-gradient procedure for selection of synchronous cells from asynchronous cultures.
A reproducible isopycnic technique, in which linear density gradients of Ficoll are used, was developed for selecting synchronous daughter cells from asynchronous cultures of Chlorella for biochemical studies of the cell cycle.
D002455:Cell Division; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D056890:Eukaryota / Q000254:growth & development*; D008722:Methods