Transfer ribonucleic acid in KB cells infected with adenovirus type 2.
Populations of transfer ribonucleic acid (tRNA) extracted from control and type 2 adenovirus (Ad2)-infected KB cells were compared. No consistent differences in acceptor activity for 11 amino acids were observed. Comparison of methylated albumin-kieselguhr (MAK) elution profiles of arginyl-tRNA from control and infected cells revealed a minor modification in that the proportion of arginyl-tRNA eluting at high salt concentration was somewhat greater in infected cells. No similar differences were observed in MAK elution profiles of aspartyl-, isoleucyl-, leucyl-, phenylalanyl-, seryl-, tyrosyl-, and valyl-tRNA. Hybridization of 4S RNA from infected cells labeled by incorporation of (3)H-uridine with Ad2 deoxyribonucleic acid revealed the presence of a complementary species of RNA in this preparation. Hybridization of (3)H-arginyl-tRNA and of (3)H-aminoacyl-tRNA labeled by charging with (3)H-arginine or a (3)H-mixture of amino acids, respectively, failed to detect the presence of virus-specific tRNA in Ad2-infected cells.
D000256:Adenoviridae / Q000254:growth & development / Q000472:pathogenicity*; D000596:Amino Acids / Q000378:metabolism; D001120:Arginine / Q000378:metabolism; D002247:Carbon Isotopes; D002277:Carcinoma; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D002845:Chromatography; D046508:Culture Techniques; D004279:DNA, Viral; D005559:Formamides; D005827:Genetics, Microbial; D006801:Humans; D006824:Hybridization, Genetic; D008025:Ligases / Q000378:metabolism; D009062:Mouth Neoplasms; D012343:RNA, Transfer / Q000378:metabolism*; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D000596:Amino Acids; D002247:Carbon Isotopes; D002712:Chlorides; D004279:DNA, Viral; D005559:Formamides; D014316:Tritium; D002586:Cesium; D012343:RNA, Transfer; D001120:Arginine; D008025:Ligases; D014529:Uridine