Active transport of glucose-1-phosphate in Agrobacterium tumefaciens.
The presence of an active transport system for glucose-1-phosphate in Agrobacterium tumefaciens was demonstrated from the following observations. (i) The bacterium could grow on a medium containing glucose-1-phosphate as carbon source; (ii) the entry of glucose-1-phosphate into the resting cells occurred against concentration gradient obeying Michaelis-Menten kinetics; and (iii) the entry reaction was energy-dependent. The transport system for glucose-1-phosphate was formed inducibly by growing the organism on a glucose-1-phosphate or sucrose medium. From the inhibition and kinetics studies it was found that the transport system had a high specificity for glucose-1-phosphate with a high affinity, K(m) value of 4.5 x 10(-6)m at pH 8.2. The existence of glucose-1-phosphate binding factor was proved in the shock fluid which was prepared from the cells grown on both glucose-1-phosphate and sucrose media by osmotic shock.
D001693:Biological Transport, Active; D002247:Carbon Isotopes; D002850:Chromatography, Gel; D003470:Culture Media; D004586:Electrophoresis; D006600:Hexosephosphates / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009995:Osmosis; D012231:Rhizobium / Q000254:growth & development / Q000378:metabolism*; D013395:Sucrose
D002247:Carbon Isotopes; D003470:Culture Media; D006600:Hexosephosphates; D013395:Sucrose