Determination of individual bile acids in biological fluids by thin-layer chromatography and fluorimetry.
A method is described for the separation and determination of individual bile acids in serum and in intestinal contents, employing thin-layer chromatography and fluorimetry. The mean recovery from intestinal juice was 94% and from serum 82%. Normal values for the individual bile acids of serum and examples of clinical applications of the method are given.
D001647:Bile Acids and Salts / Q000032:analysis* / Q000097:blood*; D002779:Cholestasis / Q000097:blood; D002855:Chromatography, Thin Layer; D005470:Fluorometry; D006505:Hepatitis / Q000097:blood; D006801:Humans; D007422:Intestines / Q000032:analysis*; D008105:Liver Cirrhosis, Biliary / Q000097:blood; D008286:Malabsorption Syndromes / Q000097:blood
D001647:Bile Acids and Salts