Gas-liquid chromatography-mass spectrometry of synthetic ceramides containing phytosphingosine.
Ceramides containing phytosphingosine as base and one of the fatty acids 16:0, 18:0, 20:0, 22:0, 23:0, and 24:0, were prepared by direct coupling in the presence of a mixed carbodiimide. The ceramides were analyzed as the 1,3,4-tri-O-trimethylsilyl ether derivatives by gas-liquid chromatography-mass spectrometry. Gas chromatographic data is presented, and structures of mass spectral ions are suggested. The structures are supported by mass spectra of the homologous ceramides, by deuterium-labeling experiments, and by high resolution mass spectrometry. Some ions, formed by cleavage between C-3 and C-4 in the long-chain base, indicate the phytosphingosine nature of the ceramide.
D000605:Amino Alcohols / Q000032:analysis / Q000138:chemical synthesis; D002849:Chromatography, Gas; D003903:Deuterium; D005227:Fatty Acids / Q000032:analysis; D006018:Glycols / Q000032:analysis / Q000138:chemical synthesis; D013057:Spectrum Analysis
D000605:Amino Alcohols; D005227:Fatty Acids; D006018:Glycols; D003903:Deuterium