Effects of rose bengal on bile secretion in the rabbit: inhibition of a bile salt-independent fraction.
The authors have studied the influence of rose bengal, and, by comparison, of uranin, on choleresis in the rabbit. Uranin induced an increase in bile flow and a decrease in bile salt concentration in bile. These results are consistent with an osmotic mechanism, as proposed by Sperber (1959). By contrast, rose bengal induced a marked decrease in bile flow and an increase in bile salt concentration. In rose bengal-treated animals (30 mumoles per kilogram body weight intravenously), bile flow was 65% lower and bile salt concentration 95% higher than in control animals studied during the same periods. The relationship between bile flow and bile salt excretion is consistent in the rabbit, with the existence of a bile salt-independent secretion of 60 mg per minute per kilogram body weight, that is, 60% of bile flow. In rose bengal-treated animals, this fraction was only 15 mg per minute per kilogram body weight. It is concluded that rose bengal inhibits the bile salt-independent fraction. Rose bengal, as other phtalein dyes, is excreted by the liver cells. Its effect on the bile salt-independent fraction suggests that this fraction is secreted by the hepatocytes.
D000818:Animals; D001646:Bile / Q000378:metabolism*; D001647:Bile Acids and Salts / Q000032:analysis / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D004396:Coloring Agents / Q000494:pharmacology; D003864:Depression, Chemical; D005704:Gallbladder / Q000187:drug effects / Q000378:metabolism; D011817:Rabbits; D012395:Rose Bengal / Q000494:pharmacology*; D012636:Secretory Rate; D013053:Spectrophotometry
D001647:Bile Acids and Salts; D004396:Coloring Agents; D012395:Rose Bengal