Environmental and nutritional factors affecting the production of rubratoxin B by Penicillium rubrum Stoll.
Rubratoxin B can be produced in a semisynthetic medium by Penicillium rubrum under varying environmental and nutritional conditions. Maximum production (552.0 mg/500 ml) was obtained with P. rubrum NRRL A-11785 grown in stationary cultures of Mosseray's simplified Raulin solution supplemented with 2.5% malt extract broth at ambient temperature. Zinc is required at levels of at least 0.4 mg per liter. In the absence of iron sulfate, there was a 50-fold reduction in rubratoxin B production but not in growth. No toxin was produced by this isolate in 5- or 7-liter fermentors.
D003470:Culture Media; D002523:Edible Grain; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D009183:Mycotoxins / Q000096:biosynthesis*; D010407:Penicillium / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology; D013696:Temperature; D015032:Zinc / Q000494:pharmacology
D003470:Culture Media; D009183:Mycotoxins; D013395:Sucrose; D007501:Iron; D008274:Magnesium; D015032:Zinc; D011188:Potassium