Galactose transport in Saccharomyces cerevisiae. II. Characteristics of galactose uptake and exchange in galactokinaseless cells.
The characteristics of the inducible galactose system in Saccharomyces cerevisiae were studied by using the nonmetabolized galactose analogues, l-arabinose and d-fucose, and galactokinaseless and transportless mutants. Induced wild-type cells transport l-arabinose by facilitated diffusion. Transportless cells transport neither galactose nor l-arabinose above the noninduced rate, whereas galactokinaseless cells transport galactose l-arabinose and d-fucose by facilitated diffusion. Determination of unidirectional rate of (14)C-labeled galactose uptake by preloaded galactokinaseless cells, containing a large unlabeled free-galactose pool, showed that the rate of galactose uptake by facilitated diffusion is greater than the rate of galactose metabolism at similar external galactose concentrations.
D001089:Arabinose / Q000378:metabolism; D001692:Biological Transport; D002247:Carbon Isotopes; D002845:Chromatography; D003470:Culture Media; D004058:Diffusion; D005374:Filtration; D005643:Fucose / Q000378:metabolism; D005690:Galactose / Q000378:metabolism*; D005827:Genetics, Microbial; D007461:Iodoacetates / Q000494:pharmacology; D009154:Mutation; D010770:Phosphotransferases / Q000378:metabolism*; D012440:Saccharomyces / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D013237:Stereoisomerism; D014501:Uranium / Q000494:pharmacology
D002247:Carbon Isotopes; D003470:Culture Media; D007461:Iodoacetates; D005643:Fucose; D014501:Uranium; D001089:Arabinose; D010770:Phosphotransferases; D005690:Galactose