Production of chlorflavonin, an antifungal metabolite of Aspergillus candidus.
Production of chlorflavonin, a new antifungal antibiotic, by strains of Aspergillus candidus is described. Two wild strains of the fungus had distinctly different chlorflavonin-producing capabilities. One strain produced 25 mug of chlorflavonin per ml per 4 to 5 days in a pilot scale fermentor with stirring, using a medium containing corn steep liquor and glucose. Production of antibiotic was favored by high rates of agitation-aeration. Crude chlorflavonin was extracted from the whole brew with a hydrocarbon solvent and then purified by recrystallization from benzene and petroleum ether. The overall yield from fermentation brew to pure product was 50%.
D000935:Antifungal Agents / Q000096:biosynthesis* / Q000302:isolation & purification; D001230:Aspergillus / Q000254:growth & development / Q000378:metabolism*; D001554:Benzene; D001681:Biological Assay; D055598:Chemical Phenomena; D002621:Chemistry; D003470:Culture Media; D005285:Fermentation; D005419:Flavonoids / Q000096:biosynthesis* / Q000302:isolation & purification; D005947:Glucose; D006863:Hydrogen-Ion Concentration; D003904:Mitosporic Fungi; D010578:Petroleum
D000935:Antifungal Agents; D003470:Culture Media; D005419:Flavonoids; D010578:Petroleum; D005947:Glucose; D001554:Benzene