Microbial growth on oxalate by a route not involving glyoxylate carboligase.
1. The metabolism of oxalate by the pink-pigmented organisms, Pseudomonas AM1, Pseudomonas AM2, Protaminobacter ruber and Pseudomonas extorquens has been compared with that of the non-pigmented Pseudomonas oxalaticus. 2. During growth on oxalate, all the organisms contain oxalyl-CoA decarboxylase, formate dehydrogenase and oxalyl-CoA reductase. This is consistent with oxidation of oxalate to carbon dioxide taking place via oxalyl-CoA, formyl-CoA and formate as intermediates, and also reduction of oxalate to glyoxylate taking place via oxalyl-CoA. 3. The pink-pigmented organisms, when grown on oxalate, contain l-serine-glyoxylate aminotransferase and hydroxypyruvate reductase but do not contain glyoxylate carboligase. The converse of this obtains in oxalate-grown Ps. oxalaticus. This indicates that, in contrast with Ps. oxalaticus, synthesis of C(3) compounds from oxalate by the pink-pigmented organisms occurs by a variant of the ;serine pathway' used by Pseudomonas AM1 during growth on C(1) compounds. 4. Evidence in favour of this scheme is provided by the finding that a mutant of Pseudomonas AM1 that lacks hydroxypyruvate reductase is not able to grow on oxalate.
D001419:Bacteria / Q000378:metabolism*; D002262:Carboxy-Lyases / Q000378:metabolism; D003065:Coenzyme A / Q000378:metabolism; D005561:Formates / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D010070:Oxalates / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D011549:Pseudomonas / Q000378:metabolism; D012694:Serine / Q000378:metabolism; D000637:Transaminases / Q000378:metabolism; D014733:Vibrio / Q000378:metabolism
D005561:Formates; D010070:Oxalates; D012694:Serine; D010088:Oxidoreductases; D000637:Transaminases; D002262:Carboxy-Lyases; D003065:Coenzyme A; D005998:Glycine