Comparison of the virion polymerase of reovirus with the enzyme purified from reovirus-infected cells.
Reovirus has in its protein coat an enzyme which catalyzes the net synthesis of the three size classes of virus-specific, single-stranded ribonucleic acid (RNA). For synthesis of 24, 19, and 14S single-stranded RNA, Mn(++) was the preferred divalent cation, and ammonium sulfate at an optimal concentration of 4.2% of saturation was an absolute requirement. During synthesis, the parental double-stranded RNA was conserved in the viral core and the newly synthesized completed RNA chains were released as free RNA. The viral cores synthesizing RNA had properties consistent with the presence of nascent RNA on their outer surface. The enzyme-template complex from the infected cells described in an earlier paper was comprised of viral cores already active in the in vivo synthesis of single-stranded RNA. This pool of viral cores was newly made during infection, and exponential increase in the number of particles in this pool, as detected by the increase in enzymatic activity, occurred 2 hr earlier than that in mature virus.
D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D002848:Chromatography, DEAE-Cellulose; D002918:Chymotrypsin / Q000494:pharmacology; D003609:Dactinomycin; D007739:L Cells; D008345:Manganese / Q000494:pharmacology; D009711:Nucleotides / Q000378:metabolism; D010759:Phosphorus Isotopes; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D012316:RNA Nucleotidyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis; D012087:Reoviridae / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development / Q000472:pathogenicity; D013395:Sucrose; D013431:Sulfates / Q000494:pharmacology; D013698:Templates, Genetic; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D014779:Virus Replication
D002712:Chlorides; D009711:Nucleotides; D010759:Phosphorus Isotopes; D000644:Quaternary Ammonium Compounds; D012367:RNA, Viral; D013431:Sulfates; D014316:Tritium; D003609:Dactinomycin; D002586:Cesium; D008345:Manganese; D013395:Sucrose; D012316:RNA Nucleotidyltransferases; D002918:Chymotrypsin; D014529:Uridine