The disulphide bonds of the heavy chain of rabbit immunoglobulin G.
Six peptides containing eight half-cystine residues were isolated in good yield, after either oxidation or reduction and carboxymethylation of fragment C-1, which contains the N-terminal half of the heavy chain of rabbit immunoglobulin G. The sequences of five of these peptides had been reported previously (Cebra, Steiner & Porter, 1968b; Wilkinson, 1969) and that of the sixth was established. Other peptides containing half-cystine residues were isolated in much lower yield and are presumed to be derived from minor sequence variants. The cystine-containing peptides from enzymic digests of whole immunoglobulin G and Fc fraction were studied by several techniques and the results obtained enable us to put forward a scheme of the arrangement of the inter- and intra-chain disulphide bonds.
D000595:Amino Acid Sequence; D000818:Animals; D001797:Blood Protein Electrophoresis; D002845:Chromatography; D003553:Cystine / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis*; D010455:Peptides / Q000032:analysis; D011817:Rabbits; D013440:Sulfides / Q000032:analysis*; D014357:Trypsin
D007074:Immunoglobulin G; D010455:Peptides; D013440:Sulfides; D003553:Cystine; D014357:Trypsin