Virus-specific proteins synthesized in cells infected with RNA+ temperature-sensitive mutants of Sindbis virus.
All Sindbis virus temperature-sensitive mutants defective in "late" functions were systematically surveyed by acrylamide-gel electrophoresis for similarities and differences in the intracellular pattern of virus-specific proteins synthesized at the permissive and nonpermissive temperatures. Only cells infected with mutants of complementation group C showed an altered pattern. At the nonpermissive temperature, these mutants failed to induce the synthesis of a polypeptide corresponding to the nucleocapsid protein and instead overproduced a protein of higher molecular weight than either viral structural protein. This defect was shown to be irreversible by the finding that (3)H-leucine incorporated at 41.5 C specifically failed to appear in the nucleocapsid of virions subsequently released at 29 C. Attempts to demonstrate a precursor protein in wild-type infections were inconclusive.
D000179:Acrylates; D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D001103:Arboviruses / Q000378:metabolism*; D002247:Carbon Isotopes; D002642:Chick Embryo; D046508:Culture Techniques; D004586:Electrophoresis; D005347:Fibroblasts; D005782:Gels; D005827:Genetics, Microbial; D006386:Hemagglutination Tests; D007106:Immune Sera; D007930:Leucine; D008970:Molecular Weight; D009154:Mutation; D014176:Protein Biosynthesis; D012333:RNA, Messenger; D012367:RNA, Viral / Q000378:metabolism*; D011817:Rabbits; D013696:Temperature; D014316:Tritium; D014764:Viral Proteins / Q000096:biosynthesis; D014776:Virus Cultivation
D000179:Acrylates; D000596:Amino Acids; D002247:Carbon Isotopes; D005782:Gels; D007106:Immune Sera; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium; D007930:Leucine