Localization of 5S RNA genes on Drosophila chromosomes by RNA-DNA hybridization.
[(3)H]RNA with a high specific activity was prepared from larvae of Drosophila melanogaster grown 4 days in contact with [(3)H]uridine. Purified tritiated 5S RNA was annealed to the DNA of polytene chromosomes, which had been denatured in formamide. The 5S RNA genes are placed within the region 56E-F of the right arm of chromosome 2. This localization was determined from autoradiographs, where the radioactivity from hybrids of [(3)H]RNA and DNA was confined to the 56E-F segment.
D000818:Animals; D001345:Autoradiography; D002875:Chromosomes / Q000032:analysis*; D004247:DNA; D004330:Drosophila / Q000378:metabolism*; D004589:Electrophoresis, Disc; D005559:Formamides; D005796:Genes; D006824:Hybridization, Genetic; D007814:Larva; D009691:Nucleic Acid Denaturation; D012313:RNA / Q000032:analysis* / Q000302:isolation & purification; D012469:Salivary Glands / Q000166:cytology; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D005559:Formamides; D014316:Tritium; D012313:RNA; D004247:DNA; D014529:Uridine