Estrogenic induction of ornithine decarboxylase in vivo and in vitro.
Injection of estrogens (17beta-estradiol or diethylstilbestrol) into immature chicks results in a marked (30- to 50-fold) increase in the ornithine decarboxylase activity of oviductal homogenates within a 4-hour period. Similar stimulations were obtained when estrogen was injected into hypophysectomized or castrated rats and the uterus was examined for decarboxylase activity. An elevation of decarboxylase activity was obtained in vitro when oviducts from immature chicks were incubated in the presence of estrogen. These data indicate a direct action of estrogen on oviduct tissue to promote a rapid increase in the activity of a specific enzyme and represent the first example of a completely in vitro enzyme response to estrogen.
D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D002247:Carbon Isotopes; D002262:Carboxy-Lyases / Q000096:biosynthesis*; D002369:Castration; D002645:Chickens; D003513:Cycloheximide / Q000494:pharmacology; D004054:Diethylstilbestrol / Q000494:pharmacology*; D004790:Enzyme Induction; D004958:Estradiol / Q000494:pharmacology*; D007016:Hypophysectomy; D066298:In Vitro Techniques; D009952:Ornithine; D010057:Oviducts / Q000201:enzymology*; D011374:Progesterone / Q000494:pharmacology; D013268:Stimulation, Chemical
D002247:Carbon Isotopes; D002245:Carbon Dioxide; D011374:Progesterone; D004958:Estradiol; D004054:Diethylstilbestrol; D003513:Cycloheximide; D009952:Ornithine; D002262:Carboxy-Lyases