A new method for the rapid isolation of chromosomes, mitotic apparatus, or nuclei from mammalian fibroblasts at near neutral pH.
D000818:Animals; D002021:Buffers; D002467:Cell Nucleus; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D002875:Chromosomes; D003124:Colorimetry; D003582:Cytogenetics; D004247:DNA / Q000032:analysis; D005347:Fibroblasts / Q000166:cytology*; D006651:Histocytochemistry; D006863:Hydrogen-Ion Concentration; D008322:Mammals; D008722:Methods; D008854:Microscopy, Electron; D008938:Mitosis; D011506:Proteins / Q000032:analysis; D012313:RNA / Q000032:analysis; D013997:Time Factors
D002021:Buffers; D011506:Proteins; D012313:RNA; D004247:DNA