The effect of preganglionic stimulation on the incorporation of L-[U-14C] valine into the protein of the superior cervical ganglion of the guinea pig.
1. Superior cervical ganglia from the guinea pig carry out an energy-dependent incorporation of l-[(14)C]valine into protein in vitro. 2. Stimulation of the preganglionic nerve at a physiological frequency for more than a few minutes decreases the ability of the ganglia to incorporate labelled valine into protein.
D000200:Action Potentials; D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D001339:Autonomic Fibers, Preganglionic / Q000502:physiology; D002247:Carbon Isotopes; D002701:Chloramphenicol / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D003864:Depression, Chemical; D004558:Electric Stimulation; D005725:Ganglia, Autonomic / Q000032:analysis / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D014176:Protein Biosynthesis; D011691:Puromycin / Q000494:pharmacology; D014633:Valine / Q000378:metabolism*
D002247:Carbon Isotopes; D003609:Dactinomycin; D011691:Puromycin; D002701:Chloramphenicol; D000255:Adenosine Triphosphate; D003513:Cycloheximide; D014633:Valine