Kinetic evidence for a (4-amino-2-methyl-5-pyrimidinyl)methyl-enzyme intermediate in the thiaminase I reaction.
D000814:Aniline Compounds; D001407:Bacillus / Q000201:enzymology; D055598:Chemical Phenomena; D002621:Chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010636:Phenols; D010847:Picolines; D011725:Pyridines; D011726:Pyridinium Compounds / Q000138:chemical synthesis; D011806:Quinolinium Compounds / Q000138:chemical synthesis; D013053:Spectrophotometry; D013438:Sulfhydryl Compounds; D013831:Thiamine; D014166:Transferases; D014316:Tritium; D014466:Ultraviolet Rays
D000814:Aniline Compounds; D010636:Phenols; D010847:Picolines; D011725:Pyridines; D011726:Pyridinium Compounds; D011806:Quinolinium Compounds; D013438:Sulfhydryl Compounds; D014316:Tritium; D014166:Transferases; D013831:Thiamine