Spectrophotometric methods for monitoring the microbial transformation of steroids. I. Determination of 9-alpha-fluorohydrocortisone and 9-alpha-fluoro-16-alpha-hydroxyhydrocortisone in fermentation broths.
A method has been devised for the determination of 9alpha-fluorohydrocortisone and 9alpha-fluoro-16alpha-hydroxyhydrocortisone in fermentation broths. The method involves extraction of the two steroids from the broth with ethyl acetate, separation through the formation of the water-soluble borate complex of the vicinally hydroxylated steroid, and estimation of each steroid spectrophotometrically.
D000085:Acetates; D001419:Bacteria / Q000378:metabolism*; D001881:Borates; D003470:Culture Media; D005285:Fermentation; D005438:Fludrocortisone / Q000032:analysis* / Q000302:isolation & purification; D008722:Methods; D012964:Sodium; D012997:Solvents; D013053:Spectrophotometry; D013256:Steroids / Q000096:biosynthesis*; D014221:Triamcinolone / Q000032:analysis* / Q000302:isolation & purification
D000085:Acetates; D001881:Borates; D003470:Culture Media; D012997:Solvents; D013256:Steroids; D014221:Triamcinolone; D012964:Sodium; D005438:Fludrocortisone