Appearance of defective virions in clones of reovirus.
Virus obtained from five plaques of reovirus was serially passaged in L cells. Defective virions arose in each clone by the seventh passage. Such defective virions lacked the largest of the 10 segments of the double-stranded ribonucleic acid genome.
D000179:Acrylates; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D002918:Chymotrypsin; D003609:Dactinomycin; D004586:Electrophoresis; D005782:Gels; D005827:Genetics, Microbial; D007739:L Cells; D012367:RNA, Viral / Q000032:analysis; D012087:Reoviridae / Q000254:growth & development* / Q000302:isolation & purification; D014316:Tritium; D014529:Uridine; D014779:Virus Replication
D000179:Acrylates; D002712:Chlorides; D005782:Gels; D012367:RNA, Viral; D014316:Tritium; D003609:Dactinomycin; D002586:Cesium; D002918:Chymotrypsin; D014529:Uridine