Method for extracting viral hemagglutination-inhibiting antibodies from the nonspecific inhibitors of serum.
Various methods are used to remove nonspecific inhibitors from sera before titering viral hemagglutination-inhibiting antibodies. These methods have several undesirable features; some are tedious and time-consuming, some remove antibody along with nonspecific inhibitors, and different techniques are usually required to remove the nonspecific inhibitors for different viruses. This communication describes a single method that uses diethylaminoethyl-Sephadex to extract the immunoglobulin G antibodies for several viruses from nonspecific inhibitors. The procedure is fast, simple to perform, and removed the nonspecific inhibitors for influenza, Western equine encephalitis, dengue-2, and rubella viruses.
D000096:Acetone; D000818:Animals; D001102:Arbovirus Infections / Q000276:immunology*; D003168:Complement Fixation Tests; D003716:Dengue Virus / Q000276:immunology; D003937:Diagnosis, Differential; D004668:Encephalitis Viruses / Q000276:immunology; D004683:Encephalomyelitis, Equine / Q000276:immunology; D005021:Ethylamines; D005260:Female; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007106:Immune Sera; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis*; D007616:Kaolin; D008297:Male; D008722:Methods; D009500:Neutralization Tests; D009975:Orthomyxoviridae / Q000276:immunology; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000276:immunology*; D011817:Rabbits; D012409:Rubella / Q000276:immunology*; D012412:Rubella virus / Q000276:immunology; D013053:Spectrophotometry
D005021:Ethylamines; D007106:Immune Sera; D007074:Immunoglobulin G; D000096:Acetone; D007616:Kaolin