Analytical study of the degradation of nucleohistone during calf thymus chromatin autolysis.
The mechanism of nucleohistone degradation in calf thymus chromatin during autolysis of the latter involves a release of lysine-rich histone fractions, followed by degradation of the thus-exposed DNA portions; the latter portions are richest in adenine-thymine sequences. After this degradation process, residual nucleohistone fragments of low molecular weight (10(5)) remain. Their chemical composition is special, in that the DNA contains a larger proportion of guanine-cytosine sequences than does the initial DNA and it is combined with arginine-rich histones.
D000225:Adenine / Q000032:analysis; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001329:Autolysis; D002417:Cattle; D003596:Cytosine / Q000032:analysis; D004247:DNA / Q000032:analysis; D004586:Electrophoresis; D006147:Guanine / Q000032:analysis; D006657:Histones / Q000032:analysis*; D008970:Molecular Weight; D009698:Nucleoproteins / Q000032:analysis*; D013941:Thymine / Q000032:analysis; D013950:Thymus Gland / Q000032:analysis*
D000596:Amino Acids; D006657:Histones; D009698:Nucleoproteins; D006147:Guanine; D003596:Cytosine; D004247:DNA; D000225:Adenine; D013941:Thymine