Sugar substrates for L-lysine fermentation by Ustilago maydis.
The extracellular production of l-lysine in media with cane sugar, blackstrap molasses, or clarified sugar-cane juice by a previously obtained mutant of Ustilago maydis was studied. Enzymatically inverted clarified juice (medium J-3) gave 2.9 g of lysine per liter under the following conditions: inoculum, 5%; pH 5.8; temperature, 30 C; K(La) in the fermentors, 0.41 mmoles of O(2) per liter per min; fermentation time, 72 hr. The concentrate, obtained by direct evaporation and drying of the fermentation broth, could be used as a possible feed supplement because of its amino-acid and vitamin content.
D001322:Autoanalysis; D001487:Basidiomycota / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D002854:Chromatography, Paper; D003470:Culture Media; D005285:Fermentation; D005827:Genetics, Microbial; D006863:Hydrogen-Ion Concentration; D008239:Lysine / Q000032:analysis / Q000096:biosynthesis*; D009154:Mutation; D010376:Pediococcus / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D013237:Stereoisomerism; D013696:Temperature
D003470:Culture Media; D000644:Quaternary Ammonium Compounds; D008239:Lysine