Density invariance of cultured Chinese hamster cells with stage of the mitotic cycle.
Isopycnic banding of Chinese hamster line CHO cells in Ficoll gradients shows that a population in balanced, exponential growth is very homogeneous with respect to density, the coefficient of variation of the density distribution spectrum being less than 5% of the mean reduced density (i.e. density minus one). Similar measurements on synchronized cultures indicate that reduced density varies by less than 2% around the life cycle. The mean density of CHO cells in F-10 growth medium is calculated to be 1.051 after correction for osmotic effects of the Ficoll gradient.
D000818:Animals; D003585:Cell Biology; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D003080:Cold Temperature; D006224:Cricetinae; D046508:Culture Techniques; D008938:Mitosis; D009994:Osmolar Concentration; D009995:Osmosis; D012965:Sodium Chloride; D013997:Time Factors
D012965:Sodium Chloride