Studies of laryngotracheitis virus in avian tissue cultures. 3. Enhancement of infectivity by diethylaminoethyl-dextran.
Diethylaminoethyl-dextran (DEAE-D) enhanced the infectivity of laryngotracheitis virus (LTV) for chicken kidney (CK) cells when cultures were treated before inoculation with virus and when DEAE-D was present in the inoculum. Infectivity was not increased when cultures were treated after virus had adsorbed to cells; since infection was not synchronized, most of the virus had probably already penetrated the plasma membrane by the time DEAE-D was added. Maximal enhancement occurred when DEAE-D was present in the inoculum. Enhancement of a lesser degree occurred when virus and DEAE-D were mixed, diluted, and inoculated onto cultures. Adsorption of LTV at 37 C as compared to that at 5 C usually yields about a threefold greater number of plaques after a 2-hr adsorption period. However, when DEAE-D was incorporated in the inoculum, greater enhancement occurred at 5 C than at 37 C, and the number of plaques produced at both adsorption temperatures was about equal. Results are compatible with the hypothesis that increased adsorption is a factor in enhancement of infectivity of LTV by DEAE-D.
D000327:Adsorption; D000818:Animals; D002642:Chick Embryo; D046508:Culture Techniques; D003911:Dextrans / Q000494:pharmacology*; D005021:Ethylamines / Q000494:pharmacology; D006564:Herpesviridae / Q000187:drug effects / Q000472:pathogenicity*; D007668:Kidney; D013696:Temperature
D003911:Dextrans; D005021:Ethylamines