Preparation and characterization of antigen and antibody adsorbents covalently coupled to an inorganic carrier.
Immunoadsorbents were prepared by the use of an inorganic carrier. The proteins were covalently coupled to the carrier through a silane coupling agent. An immunoadsorbent prepared with human gamma-globulin was found to complex with specific antibodies. These antibodies could be recovered in high yield and purity from the adsorbent. Similarly, insolubilized antibodies to l-asparaginase were used to isolate and purify the enzyme from crude extracts. These insolubilized antibodies are extremely stable with time and may be reused many times.
D000327:Adsorption; D000818:Animals; D000906:Antibodies; D000941:Antigens; D001215:Asparaginase / Q000302:isolation & purification; D002417:Cattle; D005898:Glass; D006801:Humans; D007120:Immunochemistry; D007074:Immunoglobulin G; D008722:Methods; D011485:Protein Binding; D012710:Serum Albumin, Bovine; D012995:Solubility; D014020:Tissue Extracts
D000906:Antibodies; D000941:Antigens; D007074:Immunoglobulin G; D014020:Tissue Extracts; D012710:Serum Albumin, Bovine; D001215:Asparaginase