An assessment of techniques suitable for the diagnosis of sickle-cell disease and haemoglobin C disease in cord blood samples.
Agar gel, cellulose acetate, and starch gel electrophoresis are all capable of diagnosing sickle-cell anaemia, sickle-cell haemoglobin C disease, and haemoglobin C disease in cord blood samples. Of these three electrophoretic techniques, agar gel is the easiest to interpret.Paper electrophoresis can reliably and rapidly detect sickle haemoglobin and haemoglobin C in cord blood samples. Being incapable of differentiating foetal and normal adult haemoglobin, the value of paper electrophoresis is limited to an initial screening procedure.
D000362:Agar; D000755:Anemia, Sickle Cell / Q000175:diagnosis*; D001797:Blood Protein Electrophoresis; D002482:Cellulose; D005319:Fetal Hemoglobin / Q000032:analysis; D005782:Gels; D006444:Hemoglobin C; D006445:Hemoglobin C Disease / Q000175:diagnosis; D006453:Hemoglobinopathies / Q000175:diagnosis*; D006801:Humans; D007231:Infant, Newborn; D007232:Infant, Newborn, Diseases / Q000175:diagnosis*; D010209:Paper; D013213:Starch; D014470:Umbilical Cord
D005782:Gels; D000362:Agar; D002482:Cellulose; D013213:Starch; D006444:Hemoglobin C; D005319:Fetal Hemoglobin