Studies on inhibition of intestinal absorption of radioactive strontium. IX. Relationship between biological activity and electron microscopic appearance of alginic acid components.
The inhibitory action of alginate on intestinal absorption of radioactive strontium was investigated in order to correlate the biological activity with the chemical composition. Alginate from Laminaria hyperborea was partially hydrolyzed with oxalic acid and the degradation products were fractionated into polymannuronic and polyguluronic acid. The activity of these products was assessed biologically in rats and morphologically by electron microscopy. Sodium polymannuronate was found to be less effective than sodium polyguluronate in preventing absorption of radiostrontium. The inhibition of absorption of radio-calcium was low and not affected by hydrolysis or fractionation. When dried from dilute aqueous solutions, the polymannuronate retained the original helical structure of alginate, while the polyguluronate showed a strong tendency to coagulate, forming granules. The variation in the biological activity was attributed to the morphological differences between these alginic acid components and it is suggested that the degree of uncoiling of the polyguluronate chain in water is greater than that of the polymannuronate chain, thus making the carboxylate ions more accessible to strontium.
D000464:Alginates / Q000494:pharmacology*; D000818:Animals; D001665:Binding Sites; D001681:Biological Assay; D002118:Calcium / Q000378:metabolism; D002127:Calcium Isotopes; D055598:Chemical Phenomena; D002621:Chemistry; D003864:Depression, Chemical; D006601:Hexoses / Q000494:pharmacology; D007408:Intestinal Absorption / Q000187:drug effects*; D008358:Mannose / Q000494:pharmacology; D008854:Microscopy, Electron; D051381:Rats; D012964:Sodium; D013324:Strontium / Q000378:metabolism*; D013325:Strontium Isotopes; D014574:Uronic Acids / Q000494:pharmacology*
D000464:Alginates; D002127:Calcium Isotopes; D006601:Hexoses; D013325:Strontium Isotopes; D014574:Uronic Acids; D012964:Sodium; D008358:Mannose; D002118:Calcium; D013324:Strontium