Ultrastructural studies on mitochondrial swelling.
Mitochondrial swelling induced by valinomycin, calcium chloride and P(i) was studied after potassium permanganate fixation in suspension. Valinomycin induces a rapid K(+) influx, increase of the matrix space and out-folding of the cristae, with good preservation of the matrix material. This swelling is reversible but the cristae do not completely re-form and have a blebbed appearance. On repeated swelling and contraction cycles there is a gradual loss of matrix material. Calcium chloride and P(i) produce a slow swelling of the matrix space. Shrinkage induced by ATP was partial and not associated with return to the original structure.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002122:Calcium Chloride / Q000494:pharmacology; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D008928:Mitochondria / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism; D008933:Mitochondrial Swelling / Q000187:drug effects; D010758:Phosphorus / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D051381:Rats
D000900:Anti-Bacterial Agents; D010758:Phosphorus; D000255:Adenosine Triphosphate; D002122:Calcium Chloride; D011188:Potassium