In vitro induction of a primary response to the dinitrophenyl determinant.
Primary antibody response against the dinitrophenyl group has been elicited in vitro after the stimulation of normal mouse spleen explants with 2,4-dinitrophenyl (DNP)-hemocyanin or alpha-DNP-poly-L-lysine (PLL). Antibodies were detected in the culture medium by the inactivation of DNP-T4 phage. The specificity of the reaction was manifested by the lack of the capacity of the medium to inactivate the unmodified bacteriophage and by the inhibition of the inactivation of DNP-T4 with DNP-lysine.
D000818:Animals; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D000941:Antigens; D003090:Coliphages / Q000276:immunology; D046508:Culture Techniques; D004140:Dinitrophenols / Q000494:pharmacology*; D005260:Female; D006241:Haptens; D007106:Immune Sera; D051379:Mice; D009924:Organ Culture Techniques; D013154:Spleen / Q000276:immunology*
D000941:Antigens; D004140:Dinitrophenols; D006241:Haptens; D007106:Immune Sera