Carbon dioxide fixation by mouse embryos prior to implantation.
Mouse embryos in the stage of development prior to implantation were cultured in vitro in a medium that contained radioactive bicarbonate. The radioactivity was incorporated into the proteins and nucleic acids that were acid soluble. Uptake of radioactivity occurred into protein in the unfertilized ovum and was highest in all fractions in the early blastocyst stage. No incorporation was detected in the lipid fraction.
D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism*; D002247:Carbon Isotopes; D002454:Cell Differentiation; D046508:Culture Techniques; D004622:Embryo, Mammalian / Q000378:metabolism*; D005260:Female; D005865:Gestational Age; D051379:Mice; D010063:Ovum / Q000378:metabolism
D002247:Carbon Isotopes; D002245:Carbon Dioxide