Ribonuclease sensitivity of Semliki Forest virus nucleocapsids.
Treatment of Semliki Forest virus nucleocapsids with pancreatic ribonuclease (1 mug/ml, 37 C) digests the ribonucleic acid to acid-soluble fragments; the nucleocapsid protein forms a rapidly sedimenting aggregate.
D000596:Amino Acids; D000818:Animals; D002417:Cattle; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D046508:Culture Techniques; D008854:Microscopy, Electron; D010179:Pancreas / Q000201:enzymology; D012367:RNA, Viral; D012260:Ribonucleases / Q000378:metabolism / Q000494:pharmacology*; D012672:Semliki forest virus / Q000187:drug effects*; D013395:Sucrose; D014316:Tritium; D014529:Uridine; D014764:Viral Proteins / Q000302:isolation & purification
D000596:Amino Acids; D012367:RNA, Viral; D014764:Viral Proteins; D014316:Tritium; D013395:Sucrose; D012260:Ribonucleases; D014529:Uridine