Inhibition of carotenoid hydroxylation in Staphylococcus aureus by mixed-function oxidase inhibitors.
Compounds known to be inhibitors of mixed-function oxidase systems inhibited the aerobic synthesis of hydroxylated carotenoids in Staphylococcus aureus U-71. Growth of the cells in the presence of 2-diethylaminoethyl-2,2-diphenyl valerate, 2,4-dichloro-6-phenylphenoxyethylamine, 2,4-dichloro-6-phenylphenoxyethyldiethylamine, and piperonyl butoxide reduced the levels of the rubixanthins found in stationary-phase cells by 75 to 97%. In cells grown with mevalonate-2-(14)C, the turnover rate of phytoene was reduced and the turnover rate of phytofluenol was increased in the presence of these inhibitors. The zeta- and delta-carotenes, which turn over in the absence of the inhibitors, accumulated (14)C in the presence of the inhibitors. This suggested that a mixed-function oxidase was responsible for the aerobic hydroxylation of delta-carotene in S. aureus U-71.
D002247:Carbon Isotopes; D002338:Carotenoids / Q000096:biosynthesis / Q000378:metabolism*; D002854:Chromatography, Paper; D003470:Culture Media; D004149:Dioxoles / Q000494:pharmacology; D005021:Ethylamines / Q000494:pharmacology*; D008798:Mevalonic Acid / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000037:antagonists & inhibitors; D010636:Phenols / Q000494:pharmacology; D013053:Spectrophotometry; D013210:Staphylococcus / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D014631:Valerates / Q000494:pharmacology; D014970:Xanthines / Q000096:biosynthesis
D002247:Carbon Isotopes; D003470:Culture Media; D004149:Dioxoles; D005021:Ethylamines; D010636:Phenols; D014631:Valerates; D014970:Xanthines; D002338:Carotenoids; D006899:Mixed Function Oxygenases; D008798:Mevalonic Acid