Mechanism of action of thyroid hormones on erythrocyte 2,3-diphosphoglyceric acid synthesis.
Normal erythrocytes, when incubated with thyroid hormone, were found to have increased levels of 2,3-diphosphoglyceric acid. In addition, a partially purified enzyme preparation, when incubated with either a 1,3-diphosphoglyceric acid generating system or 1,3-diphosphoglyceric acid directly, showed increased levels of 2,3-diphosphoglyceric acid when exposed to thyroid hormone. The hormonal effect was biphasic and was witnessed after 5 min of incubation. Substitution on the 3 and 5 positions of the basic thyronine molecule was essential for hormonal effect. It appears that thyroid hormone acts by directly stimulating the diphosphoglycerate mutase enzyme. The hormonal effect on 2,3-DPG synthesis may offer a biochemical explanation for the shift in the oxyhemoglobin dissociation curve observed in thyroid disorders.
D000255:Adenosine Triphosphate / Q000378:metabolism; D001665:Binding Sites; D004912:Erythrocytes / Q000378:metabolism*; D005988:Glyceric Acids / Q000096:biosynthesis*; D006019:Glycolysis; D006454:Hemoglobins; D006801:Humans; D008722:Methods; D010100:Oxygen / Q000097:blood; D010101:Oxygen Consumption; D010759:Phosphorus Isotopes; D010770:Phosphotransferases / Q000378:metabolism*; D013974:Thyroxine / Q000494:pharmacology*; D013997:Time Factors; D014284:Triiodothyronine / Q000494:pharmacology*
D005988:Glyceric Acids; D006454:Hemoglobins; D010759:Phosphorus Isotopes; D014284:Triiodothyronine; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D013974:Thyroxine; D010100:Oxygen