Segregational respiratory-deficient mutants of a "petite negative" yeast Schizosaccharomyces pombe 972h-.
No viable respiratory-deficient mutants of Schizosaccharomyces pombe 972h(-) could be obtained by acriflavine and ethidium bromide treatments. These mutagens induce 15 to 70% of microcolonies which, after a growth-lag of a few days, further develop into normal, respiratory-competent colonies. These results suggest that unstable petites were induced. Segregational respiratory-deficient mutants resistant to cobalt sulfate inhibition were isolated. Some of these strains are deficient in cytochrome a + a(3) and respire at low rates. The morphology of their mitochondrial membranes is modified: either the cristae are absent or they show aberrant concentric or tubular structures. Segregational mutants resistant to the respiratory inhibitors, 2,4-dinitrophenol or decamethylene diguanidine, were obtained. Neither mitochondrial structure nor function seems to be modified in these mutants. A segregational mutant resistant to benzimidazole inhibition does not grow on glycerol, although neither growth on glucose nor respiration appear to be affected.
D000166:Acridines; D001562:Benzimidazoles / Q000494:pharmacology; D003035:Cobalt / Q000494:pharmacology; D003580:Cytochromes / Q000378:metabolism; D004140:Dinitrophenols / Q000494:pharmacology; D005827:Genetics, Microbial; D006146:Guanidines / Q000494:pharmacology; D008854:Microscopy, Electron; D008928:Mitochondria / Q000378:metabolism; D009154:Mutation; D010101:Oxygen Consumption; D010617:Phenanthridines; D015003:Yeasts / Q000166:cytology / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism
D000166:Acridines; D001562:Benzimidazoles; D003580:Cytochromes; D004140:Dinitrophenols; D006146:Guanidines; D010617:Phenanthridines; D003035:Cobalt