Transient stimulation of deoxyribonucleic acid-dependent ribonucleic acid polymerase and histone acetylation in human embryonic kidney cultures infected with adenovirus 2 or 12: apparent induction of host ribonucleic acid synthesis.
The synthesis of cell-specific ribonucleic acid (RNA) appeared to be stimulated in human embryonic kidney (HEK) cultures infected with adenovirus 2 or 12. Deoxyribonucleic acid (DNA)-RNA hybridization experiments revealed that by 44 to 70 hr after infection with either virus, the relative amount of pulse-labeled RNA capable of hybridizing with HEK cell DNA increased considerably; such RNA was detected in both nuclear and cytoplasmic fractions. The main increase in apparent host RNA synthesis was preceded by (i) a relatively early transient stimulation of the DNA-dependent RNA polymerase activity in isolated nuclei, and (ii) a small but consistently observed increase in the rate of acetylation of lysine-rich and arginine-rich histone fractions. The Mn(2+)-(NH(4))(2)SO(4) and Mg(2+)-activated RNA polymerase reactions measured in nuclei isolated from cells infected with adenovirus 2 or 12 were stimulated at about the same time; a rapid loss of polymerase activity followed. The augmentation of the two RNA polymerase reactions found in adenovirus 12-infected cells was independent of protein synthesis. After the initial increase, the acetylation rate of histones of cells infected with adenovirus 2 or 12 declined, until late in infection it was approximately 40 to 70% of the control cell rate.
D000085:Acetates / Q000378:metabolism; D000256:Adenoviridae / Q000254:growth & development / Q000472:pathogenicity*; D002247:Carbon Isotopes; D046508:Culture Techniques; D004247:DNA; D004789:Enzyme Activation; D005827:Genetics, Microbial; D006657:Histones / Q000378:metabolism*; D006801:Humans; D006824:Hybridization, Genetic; D007477:Ions; D007668:Kidney; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D011691:Puromycin / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis*; D012316:RNA Nucleotidyltransferases / Q000378:metabolism*; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D000085:Acetates; D002247:Carbon Isotopes; D006657:Histones; D007477:Ions; D014316:Tritium; D008345:Manganese; D011691:Puromycin; D012313:RNA; D004247:DNA; D012316:RNA Nucleotidyltransferases; D008274:Magnesium; D014529:Uridine