Somatic antigens of Streptococcus group E. I. Comparison of extraction techniques.
Eleven Streptococcus group E strains, representing serotypes I, II, III, IV, V, and "untypable" isolates, were extracted by formamide, trichloroacetic acid, and hydrochloric acid under various conditions in an effort to determine the best method for recovering maximum amounts of group and type antigens. The group antigen was found to be relatively stable, and adequate amounts for identification purposes were recovered by a wide spectrum of conditions. Type-specific antigens were relatively labile, and were destroyed at low pH in acid hydrolysis or by prolonged heating in formamide hydrolysis. The best single procedure for recovering both type and group antigens from Streptococcus group E was formamide hydrolysis for 30 min at 180 C.
D000818:Animals; D000941:Antigens / Q000302:isolation & purification*; D002498:Centrifugation; D005559:Formamides; D006358:Hot Temperature; D006851:Hydrochloric Acid; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera; D008199:Lymphadenitis / Q000276:immunology / Q000662:veterinary; D008722:Methods; D012703:Serotyping; D013290:Streptococcal Infections / Q000276:immunology / Q000662:veterinary; D013291:Streptococcus / Q000276:immunology*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology; D014238:Trichloroacetic Acid
D000941:Antigens; D005559:Formamides; D007106:Immune Sera; D014238:Trichloroacetic Acid; D006851:Hydrochloric Acid