Inhibition of cytotoxicity of lymphocytes by concanavalin A in vitro.
Human lymphocytes treated with the plant protein concanavalin A are stimulated to transform into blasts, without developing cytotoxicity for chicken erythrocytes. Prior treatment of lymphocytes with concanavalin A potentiated phytohemagglutinin-induced blast transformation and DNA synthesis but completely inhibited phytohemagglutinin-induced cytotoxicity. Inhibiton was not due to suppression of the mixed lymphocyte-erythrocyte aggregation normally caused by phytohemagglutinin. Inhibition of cytotoxicity was reversible when concanavalin A was removed from the lymphocytes by treatment with methyl-alpha-D-manno-pyranoside after 1 hour but not after 20 hours. The results indicate that blast transformation and cytotoxicity are separate expressions of lymphocyte stimulation.
D000818:Animals; D002471:Cell Transformation, Neoplastic; D002645:Chickens; D002859:Chromium Isotopes; D046508:Culture Techniques; D004912:Erythrocytes / Q000187:drug effects; D037102:Lectins / Q000494:pharmacology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects*; D010940:Plant Proteins / Q000494:pharmacology*
D002859:Chromium Isotopes; D037102:Lectins; D010940:Plant Proteins