Isoelectric focusing of human liver alkaline phosphatase.
1. Isoelectric focusing of human liver alkaline phosphatase in a sucrose density gradient with LKB Ampholine as carrier ampholytes is described. 2. Problems due to the chelating properties of the ampholytes and the pH gradient were examined. 3. A reactivation procedure to counter these effects was devised that can probably be used for other alkaline phosphatases. 4. The isoelectric point of human liver alkaline phosphatase was found to be pH3.9.
D000469:Alkaline Phosphatase / Q000032:analysis*; D002021:Buffers; D004586:Electrophoresis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D008099:Liver / Q000201:enzymology*; D008722:Methods; D013395:Sucrose; D015032:Zinc
D002021:Buffers; D013395:Sucrose; D000469:Alkaline Phosphatase; D015032:Zinc