Cellular control of the synthesis and activity of the bacterial luminescent system.
In bioluminescent bacteria growing in shake flasks, the enzyme luciferase has been shown to be synthesized in a relatively short burst during the period of exponential growth. The luciferase gene appears to be completely inactive in a freshly inoculated culture; the pulse of preferential luciferase synthesis which occurs later is the consequence of its activation at the level of deoxyribonucleic acid transcription which is attributed to an effect of a "conditioning" of the medium by the growing of cells. Although cells grown in a minimal medium also exhibit a similar burst of synthesis of the luminescent system, the amount of synthesis is quantitatively less, relative to cell mass. Under such conditions, added arginine results in a striking stimulation of bioluminescence. This is attributed to a stimulation of existing patterns of synthesis and not to induction or derepression per se.
D000447:Aldehydes / Q000494:pharmacology; D000818:Animals; D001120:Arginine / Q000494:pharmacology; D002701:Chloramphenicol / Q000494:pharmacology; D003470:Culture Media; D003486:Cyanides / Q000494:pharmacology; D004790:Enzyme Induction; D008027:Light; D008156:Luciferases / Q000096:biosynthesis*; D008163:Luminescent Measurements; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation; D010776:Photobacterium / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D011817:Rabbits; D012293:Rifampin / Q000494:pharmacology
D000447:Aldehydes; D003470:Culture Media; D003486:Cyanides; D009153:Mutagens; D002701:Chloramphenicol; D001120:Arginine; D008156:Luciferases; D012293:Rifampin