Quantitative determination of neuraminidase-active foci in cell monolayer cultures infected with influenza or newcastle disease virus.
A synthetic neuraminidase substrate [2-(3'-methoxyphenyl)-N-acetyl-alpha-neuraminic acid] was used to detect myxo- and paramyxovirus replication in tissue culture. This method allowed estimation of viral growth even in the absence of cytopathogenic effects.
D000818:Animals; D002460:Cell Line; D002642:Chick Embryo