Haem a, cytochrome c and total protein turnover in mitochondria from rat heart and liver.
Haem a and cytochrome c were isotopically labelled in mitochondria from rat heart and liver after injection of delta-amino[2,3-(3)H(2)]laevulate, a specific haem precursor. [guanido-(14)C]Arginine or l-[4,5-(3)H(2)]leucine were used to label mitochondrial proteins. Half-lives were measured from biological decay in vivo and were similar (5.5-6.2 days) for haem a, cytochrome c and [(14)C]arginine-labelled proteins. Labelling of hepatic mitochondrial proteins with [(3)H(2)]leucine resulted in a prolonged apparent half-life.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001120:Arginine / Q000378:metabolism; D002247:Carbon Isotopes; D003580:Cytochromes / Q000378:metabolism*; D005260:Female; D006418:Heme / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D007982:Levulinic Acids / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism*; D008931:Mitochondria, Muscle / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D014316:Tritium
D000596:Amino Acids; D002247:Carbon Isotopes; D003580:Cytochromes; D007982:Levulinic Acids; D011506:Proteins; D014316:Tritium; D006418:Heme; D001120:Arginine; D007930:Leucine