Lipids of a sterol-nonrequiring Mycoplasma.
The lipids of the sterol nonrequiring Mycoplasma strain S743 were found to include both ester glycerophosphatides (phosphatidylglycerol, acylphosphatidylglycerol, and diphosphatidylglycerol) and ceramide glycerophosphate compounds containing N-hydroxyacyl groups. The major phosphosphingolipid was tentatively identified as a hydroxyceramidephosphorylglycerol containing an O-acyl group. These compounds became labeled during growth in the presence of (32)P-orthophosphate, (14)C-glycerol, or (14)C-palmitate. The lipid fraction also contained free long-chain base. (14)C-palmitate was converted to labeled sphinganine. The long-chain base composition of the lipids was modified by growing the organisms in media containing different fatty acids, which were converted to bases containing two more C atoms per molecule. Ninety per cent of the long-chain base from cells grown in medium supplemented with elaidate consisted of monounsaturated C(20) base.
D001345:Autoradiography; D002247:Carbon Isotopes; D055598:Chemical Phenomena; D002621:Chemistry; D002848:Chromatography, DEAE-Cellulose; D002849:Chromatography, Gas; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D003470:Culture Media; D005227:Fatty Acids / Q000032:analysis; D005990:Glycerol / Q000378:metabolism; D006017:Glycolipids / Q000032:analysis; D007259:Infrared Rays; D008055:Lipids / Q000032:analysis*; D009174:Mycoplasma / Q000032:analysis* / Q000254:growth & development / Q000378:metabolism; D009829:Oleic Acids / Q000378:metabolism; D010169:Palmitic Acids / Q000378:metabolism; D010710:Phosphates; D010759:Phosphorus Isotopes; D012997:Solvents; D013045:Species Specificity; D013057:Spectrum Analysis; D013261:Sterols / Q000378:metabolism; D014867:Water
D002247:Carbon Isotopes; D003470:Culture Media; D005227:Fatty Acids; D006017:Glycolipids; D008055:Lipids; D009829:Oleic Acids; D010169:Palmitic Acids; D010710:Phosphates; D010759:Phosphorus Isotopes; D012997:Solvents; D013261:Sterols; D014867:Water; D005990:Glycerol