Incorporation of [3H] uridine into the ribonucleic acid of rat uterus during pseudopregnancy and in the presence of I.C.I. 46474 [trans-1-(p-beta-dimethylaminoethoxyphenyl)-1,2-diphenylbut-1-ene].
1. The incorporation of [(3)H]uridine into RNA of rat uterine tissue has been measured during pseudopregnancy and in rats receiving different doses of an anti-implantation compound [trans-1-(p-beta-dimethylaminoethoxyphenyl)-1,2-diphenylbut- 1-ene, I.C.I. 46474]. 2. In the uterus of the pseudopregnant rat uridine incorporation into RNA increased markedly on day 3 of pseudopregnancy, remained high until day 5 and decreased sharply by day 6, rising again by day 9. 3. This pattern of change was similar to, but not identical with, that previously found in the pregnant rat. 4. Rats receiving I.C.I. 46474 at a dose concentration below that preventing implantation showed a pattern of RNA synthesis similar to that found in untreated control rats. 5. Rats treated with doses of I.C.I. 46474 sufficient to inhibit implantation revealed a totally different pattern of incorporation of [(3)H]uridine into uterine RNA. 6. The results are discussed in terms of previous findings with the normally pregnant rat. It is concluded that the increasing uterine RNA synthesis found on day 3 in the pregnant rat is an important requirement for the occurrence of the subsequent implantation.
D000475:Alkenes / Q000494:pharmacology; D000818:Animals; D003270:Contraceptive Agents / Q000494:pharmacology*; D004247:DNA / Q000032:analysis; D010064:Embryo Implantation / Q000187:drug effects; D005021:Ethylamines / Q000494:pharmacology*; D005260:Female; D009929:Organ Size; D011247:Pregnancy; D011555:Pseudopregnancy; D012313:RNA / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D014316:Tritium; D014529:Uridine / Q000378:metabolism*; D014599:Uterus / Q000032:analysis / Q000378:metabolism*
D000475:Alkenes; D003270:Contraceptive Agents; D005021:Ethylamines; D014316:Tritium; D012313:RNA; D004247:DNA; D014529:Uridine