Chemical properties of guinea-pig immunoglobulins gamma-1 G, gamma-2 G and gamma M.
The isolation of guinea-pig immunoglobulins gamma(1)G, gamma(2)G and gammaM are described and methods for separating the polypeptide chains of each examined. The molecular weights, extinction coefficients and carbohydrate and amino acid compositions of the immunoglobulins and their constituent chains have been analysed. The findings provide a basis for further studies attempting to relate structural differences to distinct biological properties of guinea-pig immunoglobulins.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D011232:Chemical Precipitation; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003545:Cysteine; D005643:Fucose / Q000032:analysis; D006168:Guinea Pigs; D006595:Hexosamines / Q000032:analysis; D006601:Hexoses / Q000032:analysis; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis* / Q000302:isolation & purification; D007136:Immunoglobulins / Q000032:analysis / Q000302:isolation & purification; D007461:Iodoacetates; D008623:Mercaptoethanol; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010455:Peptides / Q000032:analysis; D000644:Quaternary Ammonium Compounds; D011817:Rabbits; D014461:Ultracentrifugation
D000596:Amino Acids; D006595:Hexosamines; D006601:Hexoses; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D007461:Iodoacetates; D010455:Peptides; D000644:Quaternary Ammonium Compounds; D005643:Fucose; D008623:Mercaptoethanol; D003545:Cysteine