Microbial aromatization of steroids into equilin.
This report describes the bioconversion of 19-hydroxy-androsta-4, 7-diene-3, 17-dione into equilin with Nocardia rubra. Through mutation and improvement of medium and of conditions, the bioconversion could be improved to yield 40% equilin for a substrate concentration of 1 g/liter. Aromatization of several other 19-hydroxy and 19-nor substrates of the androstene series is reported, and the influence of various substitutions of the substrate molecule on aromatization is discussed.
D015068:17-Ketosteroids / Q000032:analysis / Q000096:biosynthesis*; D000731:Androstanes / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism*; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D003470:Culture Media; D005827:Genetics, Microbial; D007202:Indicators and Reagents; D009154:Mutation; D009615:Nocardia / Q000254:growth & development / Q000378:metabolism*; D013053:Spectrophotometry
D015068:17-Ketosteroids; D000731:Androstanes; D003470:Culture Media; D007202:Indicators and Reagents