Studies on nuclear structure and function in Tetrahymena pyriformis. II. Isolation of macro- and micronuclei.
This report describes a rapid, efficient method for isolating macronuclei from Tetrahymena. The macronuclear fraction contains only small amounts of micronuclear material and little detectable whole cell or cytoplasmic contamination. A method is also described for preparing a "micronuclear fraction" which contains 20-40 micronuclei for every macronucleus present. Electron microscope observations indicate that the ultrastructure of the nuclei in the macronuclear fraction closely resembles that of nuclei in situ. The presence of ribosomes on the outer membrane of micronuclei and of pores in the micronuclear envelope is also described.
D000818:Animals; D002466:Cell Nucleolus; D002467:Cell Nucleus / Q000032:analysis / Q000502:physiology; D002498:Centrifugation