Tamm-Horsfall urinary glycoprotein. The subunit structure.
1. Subunit molecular weights of 76000-82000 were obtained for native and alkylated Tamm-Horsfall glycoprotein by gel filtration on Sephadex G-200 in the presence of sodium dodecyl sulphate. 2. A further estimate of the subunit molecular weight of 79000+/-4000 was obtained by disc gel electrophoresis in sodium dodecyl sulphate. 3. A minimum value of the chemical molecular weight of 79000+/-6000 was obtained from the number of N-terminal amino acids released by cyanogen bromide cleavage of the glycoprotein. 4. Similar values were obtained for the subunit molecular weight of Tamm-Horsfall glycoprotein from patients with cystic fibrosis. 5. On ultracentrifugation both in 1.0% sodium dodecyl sulphate and in 70% formic acid, Tamm-Horsfall glycoprotein sedimented as a single component, slightly faster than serum albumin. 6. On reduction of the disulphide bonds the same subunit molecular weight was obtained, which suggested that these bonds are intrachain.
D000328:Adult; D000596:Amino Acids / Q000032:analysis; D002850:Chromatography, Gel; D003486:Cyanides; D003550:Cystic Fibrosis / Q000652:urine; D003902:Detergents; D003911:Dextrans; D004589:Electrophoresis, Disc; D005561:Formates; D006023:Glycoproteins / Q000032:analysis / Q000652:urine*; D006801:Humans; D008297:Male; D008970:Molecular Weight; D010084:Oxidation-Reduction; D012709:Serum Albumin; D013440:Sulfides; D014461:Ultracentrifugation
D000596:Amino Acids; D003486:Cyanides; D003902:Detergents; D003911:Dextrans; D005561:Formates; D006023:Glycoproteins; D012709:Serum Albumin; D013440:Sulfides