Incorporation of deoxyribonucleic acid precursors by T4 deoxyribonucleic acid-protein complexes retained on glass fiber filters.
Bacteriophage T4 deoxyribonucleic acid (DNA)-protein complexes were retained preferentially on glass fiber filters. DNA polymerase activity in the complex was detected through the incorporation of (3)H-labeled DNA precursors. The primer-product DNA hybridized with both phage and Escherichia coli DNA. Density labeling experiments showed that about 30% of incorporated (3)H-deoxyadenosine triphosphate was found in DNA which hybridized with phage DNA; this DNA was found to be covalently attached to the primer DNA.
D002499:Centrifugation, Density Gradient; D003090:Coliphages / Q000201:enzymology / Q000378:metabolism*; D004254:DNA Nucleotidyltransferases / Q000032:analysis; D004269:DNA, Bacterial / Q000302:isolation & purification
D004269:DNA, Bacterial; D004254:DNA Nucleotidyltransferases