Decreased ribonucleic acid synthesis in isolated rat liver nuclei during starvation.
1. Isolated nuclei from starved rats showed a lowered incorporation of [(14)C]UMP into RNA. 2. The Mg(2+)-dependent incorporation was decreased by 30% after 1 day of starvation, but incorporation in the presence of Mn(2+) and ammonium sulphate decreased only after longer periods of starvation. 3. RNA synthesis by nuclei in the presence of excess of added RNA polymerase was unchanged after 1 day of starvation and was inhibited by 20% after 4 days. 4. The capacity of nuclei to bind actinomycin D was unchanged after 1 day and was decreased by 20% after 4 days of starvation.
D000818:Animals; D002247:Carbon Isotopes; D002467:Cell Nucleus / Q000378:metabolism*; D003609:Dactinomycin / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism; D008297:Male; D008345:Manganese / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis*; D012316:RNA Nucleotidyltransferases / Q000378:metabolism; D051381:Rats; D013217:Starvation / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism; D014500:Uracil Nucleotides / Q000378:metabolism
D002247:Carbon Isotopes; D000644:Quaternary Ammonium Compounds; D013431:Sulfates; D014500:Uracil Nucleotides; D003609:Dactinomycin; D008345:Manganese; D012313:RNA; D012316:RNA Nucleotidyltransferases; D008274:Magnesium