Tamm-Horsfall urinary glycoprotein. The chemical composition.
1. A revised amino acid and carbohydrate composition of human Tamm-Horsfall glycoprotein is presented. 2. No significant differences were obtained in the amino acid composition of Tamm-Horsfall glycoprotein isolated from patients with cystic fibrosis. 3. The glycoprotein was shown to possess a high half-cystine content of 1 per 11-12 amino acid residues, which has been confirmed by performic acid oxidation and S-alkylation with iodoacetate and iodoacetamide. No thiol groups were detected in the glycoprotein. 4. Treatment of the glycoprotein with 0.5m-sodium hydroxide at 4 degrees C for 2 days did not release heterosaccharide material, which suggests that the predominant carbohydrate-protein linkages present are not of the O-glycosidic type. 5. No N-terminal amino acid was detected in the glycoprotein.
D000478:Alkylation; D000596:Amino Acids / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D003550:Cystic Fibrosis / Q000652:urine; D003553:Cystine / Q000032:analysis; D005561:Formates; D006023:Glycoproteins / Q000032:analysis / Q000652:urine*; D006027:Glycosides; D006801:Humans; D007461:Iodoacetates; D008297:Male; D010084:Oxidation-Reduction; D012972:Sodium Hydroxide; D013438:Sulfhydryl Compounds / Q000032:analysis
D000596:Amino Acids; D002241:Carbohydrates; D005561:Formates; D006023:Glycoproteins; D006027:Glycosides; D007461:Iodoacetates; D013438:Sulfhydryl Compounds; D003553:Cystine; D012972:Sodium Hydroxide