Immunization of mice with components of Pasteurella multocida.
Log-phase cells of Pasteurella multocida strain P-1059 were used to prepare isolated culture filtrate, cell wall, and cytoplasmic components. Culture filtrate was further separated by column chromatography. A portion of cytoplasm and culture filtrate was conjugated to ferritin by means of metaxylylene diisocyanate. Cell walls induced more protection in mice than the conjugated or unconjugated cytoplasm or culture filtrate. The cell walls caused edema and erythema when given intradermally in rabbits, whereas cytoplasm and culture filtrate produced dermal necrosis. The first of four chromatographically separated fractions of culture filtrate was possibly more immunogenic in mice than cell walls. This fraction was less reactive intradermally in rabbits than cell walls but more reactive than the other fractions.
D000179:Acrylates; D000818:Animals; D002473:Cell Wall / Q000276:immunology*; D002845:Chromatography; D003593:Cytoplasm / Q000276:immunology*; D004586:Electrophoresis; D005293:Ferritins; D005374:Filtration; D005620:Freund's Adjuvant; D005782:Gels; D007114:Immunization; D007120:Immunochemistry; D007279:Injections, Subcutaneous; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D010325:Pasteurella / Q000276:immunology*; D010326:Pasteurella Infections / Q000517:prevention & control*; D011817:Rabbits; D012882:Skin Tests; D014461:Ultracentrifugation; D014732:Vibration; D014867:Water
D000179:Acrylates; D005782:Gels; D014867:Water; D005293:Ferritins; D005620:Freund's Adjuvant