Comparison of immunoadsorbents prepared by coupling sperm-whale myoglobin to a variety of insoluble polymers.
Immunoadsorbents that were potentially suitable for the fractionation of antibodies to myoglobin were prepared by coupling myoglobin to aminoethylcellulose, bromoacetylcellulose, CM-cellulose, an ethylene-maleic anhydride co-polymer and Sepharose 4B. A comparison of the properties of the preparations indicated that Sepharose-myoglobin was superior, as an immunoadsorbent, to the other conjugates. Elution of adsorbed antibodies with m-propionic acid, pH2.5, gave yields of precipitable antibody higher than those achieved with other dissociating solvents. Propionic acid eluted 60-100% of the antibody adsorbed by Sepharose-myoglobin, and 60-90% of the eluted protein was precipitable antibody.
D000327:Adsorption; D000812:Anhydrides; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D002482:Cellulose; D002591:Cetacea; D005030:Ethylenes; D006863:Hydrogen-Ion Concentration; D008747:Methylcellulose; D009211:Myoglobin; D011108:Polymers; D011134:Polysaccharides; D011233:Precipitin Tests; D011422:Propionates; D011817:Rabbits
D000812:Anhydrides; D000906:Antibodies; D005030:Ethylenes; D009211:Myoglobin; D011108:Polymers; D011134:Polysaccharides; D011422:Propionates; D002482:Cellulose; D008747:Methylcellulose