title: Stabilities of suspensions of influenza virus dried by sublimation of ice in vacuo to different contents of residual moisture and sealed under different gases.
abstract: Suspensions of influenza virus were dried by sublimation of ice in vacuo to contents of residual moisture of 2.8, 1.6, or 0.6%. After drying, the preparations were sealed under helium, argon, nitrogen, or a mixture of oxygen and nitrogen (30% O(2), 70% N(2)). Stabilities of the several preparations were determined by an accelerated storage test. Based on the times predicted for the dried preparations stored at preselected temperatures to lose 1 log of infectivity titer, the order of stabilities in relation to residual moisture, gas was as follows: 1.6%, He > 0.6%, He > 2.8%, He > 1.6%, Ar > 2.8%, N(2) > 2.8%, Ar > 1.6%, N(2) > 2.8%, O(2) > 0.6%, Ar > 0.6%, N(2) > 1.6%, O(2) > 0.6%, O(2). The stability of the preparation sealed under helium and dried to the content of residual moisture found best for this gas (1.6%) resulted in an increased stability of the order of years as compared to the other preparations tested.
mesh_terms: D001128:Argon; D005612:Freeze Drying; D005740:Gases; D006371:Helium; D007053:Ice; D008722:Methods; D009584:Nitrogen; D009975:Orthomyxoviridae / Q000032:analysis / Q000472:pathogenicity; D010100:Oxygen; D011309:Preservation, Biological; D013997:Time Factors; D014867:Water / Q000032:analysis
chemical_list: D005740:Gases; D007053:Ice; D014867:Water; D006371:Helium; D001128:Argon; D009584:Nitrogen; D010100:Oxygen