Determination of the optimal priming dose for achieving an isotopic steady state in a two-pool system: application to the study of cholesterol metabolism.
The daily administration of labeled cholesterol to humans or animals leads to an isotopic steady state. The specific activity of plasma cholesterol in the isotopic steady state gives information about the fraction of plasma cholesterol derived from endogenous and exogenous sources. A method, based on a two-pool model, is presented which allows the estimation of an optimal priming dose of labeled cholesterol whereby the time to reach the isotopic steady state is reduced to a minimum. A graphic procedure is presented which allows the estimation of an optimal priming dose for two-compartment systems with widely differing characteristics.
D000284:Administration, Oral; D002784:Cholesterol / Q000008:administration & dosage / Q000097:blood* / Q000378:metabolism; D006801:Humans; D007275:Injections, Intravenous; D007700:Kinetics; D008433:Mathematics; D008722:Methods; D008954:Models, Biological; D011868:Radioisotopes
D011868:Radioisotopes; D002784:Cholesterol