title: The effects of oestradiol on the acid-soluble nucleotides of rat uterus.
abstract: 1. Techniques have been developed to measure the concentrations of the ribonucleotides of the immature rat uterus in vivo. Tissue was frozen rapidly in liquid nitrogen, ground to a fine powder, dispersed in frozen perchloric acid and thawed slowly. Nucleotides were separated from other acid-soluble constituents on short columns of polyethyleneimine-cellulose and the mixture was resolved into individual nucleotides by two-dimensional thin-layer chromatography on polyethyl-eneimine-cellulose plates. 2. The nucleotides of immature rat uterus consisted of approximately 75% of ATP-ADP, 10-12% each of GTP-GDP and UTP-UDP and less than 2% of CTP. 3. Injection of oestradiol (5mug) promoted a linear decrease in the amounts of purine nucleotides to approximately 60% of control values in 4-5h, followed by a return to greater than control values in 8-10h. Concentrations of the pyrimidine nucleotides remained constant for 4-6h and then increased to 200% of control at 12h after hormone treatment.
mesh_terms: D000227:Adenine Nucleotides / Q000032:analysis; D000818:Animals; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D003597:Cytosine Nucleotides / Q000032:analysis; D003864:Depression, Chemical; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006150:Guanine Nucleotides / Q000032:analysis; D009711:Nucleotides / Q000032:analysis*; D051381:Rats; D013268:Stimulation, Chemical; D014500:Uracil Nucleotides / Q000032:analysis; D014599:Uterus / Q000032:analysis / Q000187:drug effects*
chemical_list: D000227:Adenine Nucleotides; D003597:Cytosine Nucleotides; D006150:Guanine Nucleotides; D009711:Nucleotides; D014500:Uracil Nucleotides; D004958:Estradiol