A human tissue culture cell line from a transitional cell tumour of the urinary bladder: growth, chromosone pattern and ultrastructure.
Cell cultures were made from 18 human bladder tumours. Three cell lines were maintained for seven transfer generations, but all had a "fibroblastic" morphology and a normal diploid karyotype. A fourth line has been maintained for over 80 transfer generations. This was derived from a well differentiated papillary tumour of bladder. Morphologically the light and electron microscopic structure of the cells resembled that of bladder tumours. The cells formed tumour nodules, with a similar structure, when transplanted into hamster cheek pouches. There is a stem line chromosome number of 48. Karyotypes of 60% of the stem line cells had one extra chromosome in Group C and one in Group D.
D000818:Animals; D002295:Carcinoma, Transitional Cell / Q000235:genetics* / Q000473:pathology; D002460:Cell Line; D002875:Chromosomes; D002901:Chromosomes, Human, 13-15; D002906:Chromosomes, Human, 6-12 and X; D006224:Cricetinae; D046508:Culture Techniques; D005347:Fibroblasts; D006801:Humans; D007621:Karyotyping; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous; D001743:Urinary Bladder / Q000166:cytology / Q000473:pathology*; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology