title: Interctions between potassium ions and glycine transport in the yeast Saccharomyces carlsbergensis.
abstract: A study has been made of the effects of both varying the pH and extracellular [K(+)] on the initial rate of uptake of glycine (v) by a strain of Saccharomyces carlsbergensis that concentrated the amino acid, with respect to the extracellular phase, by up to 1400 times. When no other substrate than glycine was provided and [glycine] was relatively small (</=0.2mm) (1) v increased fivefold when the pH was lowered from 7 to 4.5; (2) v fell by up to about 80% as [K(+)] rose, K(+) behaving as a non-competitive inhibitor of the system, with K(i) 0.33mequiv./l at pH7; (3) the absorption of glycine caused up to about 2 or 3 equiv. of K(+) to leave the yeast cells. These three phenomena were each less evident when glucose was present. An analogy is drawn between the respective interactions of H(+) and K(+) with the yeast system and the well recognized effects of Na(+) and K(+) on amino acid transport in certain mammalian systems.
mesh_terms: D001224:Aspartic Acid / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects*; D002247:Carbon Isotopes; D003864:Depression, Chemical; D005947:Glucose / Q000378:metabolism; D005998:Glycine / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008239:Lysine / Q000378:metabolism; D011188:Potassium / Q000378:metabolism / Q000494:pharmacology*; D012440:Saccharomyces / Q000378:metabolism*; D012964:Sodium / Q000494:pharmacology
chemical_list: D002247:Carbon Isotopes; D001224:Aspartic Acid; D006859:Hydrogen; D012964:Sodium; D005947:Glucose; D008239:Lysine; D011188:Potassium; D005998:Glycine