The development of the dorsal and ventral mammalian pancreas in vivo and in vitro.
The origin, morphogenesis, and biochemical differentiation of the dorsal and ventral pancreas of the rat embryo have been investigated in order to ascertain the similarities and dissimilarities between the two lobes. We have utilized a culture system in which the primitive gut gives rise to a number of differentiated organs, including the dorsal and ventral pancreas. The two pancreases do not undergo fusion in these cultures, thus allowing independent analyses of the two lobes for comparison with in vivo results. The dorsal pancreas first appeared at the 23-25 somite stage while the ventral pancreas appeared approximately 12 hr later at the 29-30 somite stage. Guts from embryos as young as 12 somites were capable of developing both pancreases in vitro. In spite of the 12 hr difference between the times of their appearance, the dorsal and ventral pancreases exhibited identical patterns of morphological and biochemical differentiation. The two lobes contained the same exocrine enzymes and hormones, at similar levels, differing only in their glucagon content, the dorsal pancreas possessing a fivefold higher glucagon specific activity. The implications of these results are discussed.
D000681:Amylases / Q000032:analysis; D000818:Animals; D002268:Carboxypeptidases / Q000032:analysis; D002454:Cell Differentiation; D002918:Chymotrypsin / Q000032:analysis; D003470:Culture Media; D046508:Culture Techniques; D004707:Endoderm / Q000166:cytology; D004792:Enzyme Precursors / Q000032:analysis; D005260:Female; D005934:Glucagon / Q000032:analysis; D006651:Histocytochemistry; D007328:Insulin / Q000032:analysis; D008297:Male; D008854:Microscopy, Electron; D009024:Morphogenesis; D010179:Pancreas / Q000032:analysis / Q000166:cytology / Q000196:embryology* / Q000201:enzymology; D051381:Rats; D012260:Ribonucleases / Q000032:analysis; D013997:Time Factors
D003470:Culture Media; D004792:Enzyme Precursors; D007328:Insulin; D005934:Glucagon; D012260:Ribonucleases; D000681:Amylases; D002268:Carboxypeptidases; D002918:Chymotrypsin