An improved spray reagent for detection of bile acids on thin-layer chromatoplates.
A spray reagent containing 8-hydroxy-1,3,6-pyrenetrisulfonic acid sodium salt is described, which allows detection of as little as 1 micro g of bile acids under long-wave UV light. The bile acids can be eluted directly from the sprayed plates with acetone without eluting the spray reagent.
D001647:Bile Acids and Salts; D002855:Chromatography, Thin Layer; D005453:Fluorescence; D007202:Indicators and Reagents; D013451:Sulfonic Acids
D001647:Bile Acids and Salts; D007202:Indicators and Reagents; D013451:Sulfonic Acids