Inhibition of nucleic acid and protein synthesis mouse spleen cells in vitro by azathioprine.
The effect of azathioprine on macromolecular biosynthesis was studied in mouse spleen cells cultured in vitro. The rate of incorporation of (3)H-thymidine, (3)H-uridine, and (14)C-leucine into acid-insoluble material was used to measure deoxyribonucleic acid, ribonucleic acid, and protein synthesis. Results indicate that azathioprine inhibited nucleic acid and protein synthesis at levels which did not decrease cell viability.
D000818:Animals; D001379:Azathioprine / Q000494:pharmacology*; D002247:Carbon Isotopes; D002452:Cell Count; D004247:DNA / Q000096:biosynthesis*; D003864:Depression, Chemical; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D066298:In Vitro Techniques; D007930:Leucine / Q000378:metabolism; D051379:Mice; D011506:Proteins / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis*; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D002247:Carbon Isotopes; D011506:Proteins; D014316:Tritium; D012313:RNA; D004247:DNA; D007930:Leucine; D001379:Azathioprine; D013936:Thymidine; D014529:Uridine; D004121:Dimethyl Sulfoxide